SYNTHESIS OF A SCA-TM PROTEIN WITH ENZYMATIC ACTIVITY

Information

• Research Project
• 3498448

• ApplicationId

  3498448

• Core Project Number

  R43GM043678

• Full Project Number

  1R43GM043678-01

• Serial Number

  43678

• FOA Number

• Sub Project Id

• Project Start Date

  1/1/1990 - 35 years ago

• Project End Date

  9/30/1990 - 34 years ago

• Program Officer Name

• Budget Start Date

  1/1/1990 - 35 years ago

• Budget End Date

  9/30/1990 - 34 years ago

• Fiscal Year

  1990

• Support Year

  1

• Suffix

• Award Notice Date

  12/29/1989 - 35 years ago

Organizations

• Enzon Labs Inc.

A Single-Chain Antibody (SCA) protein with catalytic activity will be prepared. The SCA protein will be designed form the variable region sequences of one of two antibodies found to have lipase activity, either 2H6 ro 21H3. A gene to synthesize this protein will be constructed and the protein will be expressed in E. coli. The protein will be renatured, purified, and assayed for its enzymatic activity. This SCA protein will be engineered to have increased stability and increased catalytic activity during Phase II of the project. The SCA protein with catalytic activity provides the means to accomplish genetic changes and determine the effect of catalytic activity and stability.

IC Name

• Activity

  R43

• Administering IC

  GM

• Application Type

  1

• Direct Cost Amount

• Indirect Cost Amount

• Total Cost

• Sub Project Total Cost

• ARRA Funded

• CFDA Code

  821

• Ed Inst. Type

• Funding ICs

• Funding Mechanism

• Study Section

  SSS

• Study Section Name

• Organization Name

  ENZON LABS

• Organization Department

• Organization DUNS

• Organization City

  GAITHERSBURG

• Organization State

  MD

• Organization Country

  UNITED STATES

• Organization Zip Code

  20877

• Organization District

  UNITED STATES