Patent Grant
9475757
This application is a 35 USC §371 National Stage application of International Application No. PCT/IN2014/000294 filed May 2, 2014, now pending; which claims the benefit under 35 USC §119(a) to India Application Serial No. 1307/DEL/2013 filed May 3, 2013, now expired. The disclosure of each of the prior applications is considered part of and is incorporated by reference in the disclosure of this application.
The present invention relates to an improved process for synthesis of an anti-Parkinson compound of formula I from commercially available (R)-benzyl glycidyl ether. The compound produced by the process have high enantiopurity of greater than 98% (>98% ee).
Parkinson's disease (PD) is a progressive neurodegenerative disease characterized by bradykinesia, rigidity, resting tremor, and ataxia. These symptoms are caused by decreased dopamine release in the striatum. Clinically, PD is defined by presence of Lewy bodies, intracellular neuronal inclusions in the substantia nigra and at other sites in the brain. Estimated prevalence of this disease is 100 to 200 per 100,000 population including males and females across the entire age group. Current treatment for PD comprises dopaminergic medications that include levodopa, dopamine agonists (DAs), monoamine oxidase-B (MAO-B) inhibitors.
WO1998003472 discloses serinamide, glycinamide, alaninamide and phenylalaninamide derivatives of a compound (I). These compounds (I) are useful for the treatment of neurological diseases.
EP2474521 discloses high purity degree (S)-2-[4-(3-fluorobenzyloxy)-benzylamino]propanamide (safinamide) or (S)-2-[4-(2-fluorobenzyloxy)-benzylamino]propanamide (ralfinamide) or a salt thereof with a pharmaceutically acceptable acid with a content of the respective impurity (S)-2-[3-(3-fluorobenzyl)-4-(3-fluorobenzyloxy)-benzylamino]propanamide or (S)-2-[3-(2-fluorobenzyl)-4-(2-fluorobenzyloxy)-benzylamino]propanamide.
US2009149544 relates to novel alpha-aminoamide derivatives, their pharmaceutically acceptable salts, solvates, and hydrates thereof. The application also provides compositions comprising a compound and the use of such compositions in methods of treating diseases and conditions that are beneficially treated by administering an inhibitor of monoamine oxidase type B (MAO-B) and/or a sodium (Na.sup.+) channel blocker, and/or a calcium (Ca.sup.2+) channel modulator.
The strategy employed in the art to prepare benzyloxy-benzylamine derivatives including safinamide or its analogue ralfinamide is chiral pool approach starting from L-alaninamide and reductively aminating with 4-(3-fluorobenzyloxy)benzaldehyde. Although this method is very simple and straightforward, it suffers from several serious drawbacks, such as need to use toxic reagents such as sodium cyanoborohydride and further formation of toxic by-products such as hydrogen cyanide and sodium cyanide and other toxic impurities in large-scale production Importantly, the possibility of generating a range of safinamide analogues by means of the chiral-pool approach is limited in terms of the structure and stereochemistry of the products because of inadequacies in the availability of D-alaninamide and its analogues
Hence, the developments of newer methods for the preparation of compounds of formula (I) comprising safinamide and related analogues are highly desirable.
The main object of the present invention is to provide an improved and efficient process for synthesis of an anti-Parkinson compound of formula (I) using commercially available substrate with enantiopurity greater than 98%.
Another object of the present invention is to provide an improved and efficient process for synthesis of safinamide.
Yet another object of the present invention is to provide an improved and efficient process for synthesis of ralfinamide.
The present invention relates to an efficient a process for preparation of an enantiomer of compound of formula I
wherein R1 and R2 are each independently selected from hydrogen or halogen, said process comprising;
The present invention further relates to an enantiomer of compound of formula I prepared by the process for use in treatment of Parkinson's disease.
The present invention discloses a process for preparation of an enantiomer of compound of formula I
wherein R1 and R2 are each independently selected from hydrogen or halogen, said process comprising;
In an embodiment of the present invention, there is provided a process for preparation of an enantiomer of compound of formula I, wherein the enantiomer of compound of Formula I is salified with methane sulphonic acid to obtain a salt of said compound.
In yet another embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein the reducing agent is a hydride reducing agent selected from the group consisting of LiAlH4, NaBH4, Zn(BH4)2 and borane.
In still another embodiment of the present invention, there is provided a process for preparation of an enantiomer of compound of formula I, wherein the base used in step (d) and step (g) is an organic base selected from the group consisting of ethylamine, diethylamine and pyridine; or an inorganic base selected from the group consisting of alkali or alkaline metal carbonates or bicarbonates.
In another embodiment of the present invention, there is provided a process for preparation of an enantiomer of compound of formula I, wherein the base used in step (d) and step (g) is an organic base selected from the group consisting of ethylamine, diethylamine and pyridine.
In yet another embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein the base used in step (d) and step (g) is an inorganic base selected from the group consisting of alkali or alkaline metal carbonates or bicarbonates.
In still another embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein the solvent used in step (d), step (f) and step (g) is a polar aprotic solvent selected from the group consisting of acetonitrile, THF, DCM, ethyl acetate and DMF.
In another embodiment of the present invention, there is provided a process for preparation of alkyl iodide (compound 9), said process comprising;
In an embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein the compound of formula (I) is safinamide.
In another embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein the compound of formula (I) is ralfinamide.
In yet another embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein said compound obtained has an enantiopurity greater than 98%.
In still another embodiment of the present invention there is provided a process for preparation of an enantiomer of compound of formula I, wherein the yield of the enantiomer of compound of formula I is in the range of 85% to 90%.
An embodiment of the present invention provides an enantiomer of compound of formula I prepared by the process of the present invention for use in treatment of Parkinson's disease.
The present invention provides Safinamide with >98% ee. The structure of safinamide was confirmed by its IR, 1H NMR, 13C NMR and mass spectroscopic analysis. The enantiomeric purity of safinamide was determined by chiral HPLC analysis.
The invention will now be illustrated with help of examples. The aforementioned embodiments and below mentioned examples are for illustrative purpose and are not meant to limit the scope of the invention. Various modifications of aforementioned embodiments and below mentioned examples are readily apparent to a person skilled in the art.
Solvents were purified and dried by standard procedures prior to use. 1H NMR and 13C NMR spectra were recorded on a Bruker AC-200 NMR spectrometer. Spectra were obtained in CDCl3. The reactions were monitored by using TLC plates Merck Silica Gel 60 F254 and visualization with UV light (254 and 365 nm), KMnO4 and anisaldehyde in ethanol as development reagents. Optical rotations were measured with a JASCO P 1020 digital polarimeter. Enantiomeric excesses were determined by chiral HPLC, performed on ‘SHIMADZU SCL-10A unit’ system controller and UV monitor as detector.
To a solution of (R)-benzyl glycidyl ether [(R)-compound 2] (4 g, 24.4 mmol) in dry THF (10 mL) at 0° C., a pre-cooled solution of lithium aluminium hydride (1.4 g, 36.6 mmol) in anhydrous THF (10 mL) was added slowly with stirring under nitrogen. After 60 min, the reaction mixture was quenched with 1 ml of water and 1 ml of 15% NaOH solution and the content was stirred for 15 min. The inorganic precipitate was filtered, washed with ethyl acetate and the solvent evaporated under reduced pressure. The residue was purified by a short filtration column to afford (R)-compound 3 as a colorless oil (3.8 g, 95%); [α]22D=−14.5 (c 2, CHCl3); IR (CHCl3): νmax 3418, 3087, 3063, 3030, 2963, 2924, 1952, 1873, 1600, 1495, 1454, 1363, 1244, 1099, 1028, 918, 808, 698 cm−1; 1H NMR (200 MHz, CDCl3): δH 1.13 (d, J=6.3 Hz, 3H), 2.5 (bs, 1H), 3.23-3.32 (dd, J=9.8, 1.3 Hz, 1H), 3.43-3.49 (dd, J=9.45, 3.2 Hz, 1H), 3.91-4.03 (m, 1H), 4.55 (s, 2H), 7.25-7.37 (m, 5H); 13C NMR (50 MHz, CDCl3): δC 137.8 (C), 128.3 (CH, 2 carbons), 127.7 (CH, 3 carbons), 75.7 (CH2), 73.2 (CH2), 66.4 (CH), 18.6 (CH3); MS: m/z 189 [M+Na]+.
To a stirred solution of secondary alcohol (R)-compound 3 (3 g, 18.1 mmol) in dry dichloromethane (25 mL), Et3N (3.1 mL, 21.7 mmol) at 0° C. was added, followed by drop wise addition of mesyl chloride (1.8 mL, 21.7 mmol). The reaction mixture was stirred at 0° C. for 2 hours, subsequently at room temperature for 3 hours under a nitrogen atmosphere. After completion of the reaction (indicated by TLC), the reaction mixture was diluted with dichloromethane and washed with a saturated solution of sodium bicarbonate (30 mL) and water (2×10 mL). The organic layer was separated, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure to give the O-mesyl compound (4.3 g; crude).
To a solution of the crude O-mesyl compound (4 g, 16.37 mmol) in dry DMF (10 mL), sodium azide (1.6 g, 24.55 mmol) was added and the reaction mixture was heated at 60° C. for 6 hours under nitrogen atmosphere. After completion of the reaction (indicated by TLC), water (10 mL) was added to the reaction mixture, then extracted with ethyl acetate (2×15 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 95:5) to yield (S)-compound 4 as a colorless oil. (2.8 g; 89%); [α]22D=+6.1 (c 1.3, CHCl3); IR (CHCl3): νmax 3394, 3032, 2977, 2864, 2500, 2104, 1724, 1641, 1496, 1454, 1363, 1269, 1101, 913, 698 cm−1, 1H NMR (200 MHz, CDCl3): δH 1.20 (d, J=6.7 Hz, 3H), 3.39-3.54 (m, 2H), 3.61-3.77 (m, 1H), 4.57 (s, 2H), 7.25-7.39 (m, 5H); 13C NMR (50 MHz, CDCl3): δC 137.8 (C), 128.4 (CH, 2 carbons), 127.7 (CH), 127.5 (CH, 2 carbons), 73.7 (CH2), 73.2 (CH2), 56.9 (CH), 16.1 (CH3); MS: m/z 214 [M+Na]+.
To a solution of (S)-compound 4 (2.5 g, 13.1 mmol) in methanol (15 mL), trifluoroacetic acid (2 mL) and palladium hydroxide on activated carbon (0.05 g, 10-20 wt %) were added and the reaction mixture was stirred under hydrogen (60 psi) for 8 hours. After completion of the reaction (indicated by TLC), the catalyst was filtered over a plug of celite and the solvent was evaporated under reduced pressure to half of its volume which was basified with 2.5 M methanolic NaOH. Evaporation of the remaining solvent under reduced pressure was done followed by filtration of the residue through a short bed of basic alumina (eluent; MeOH) to obtain the amino alcohol as a pale brown oil (0.94 g, crude) which was subjected to the next reaction without further purification.
To a solution of amino alcohol (0.9 g, 11.98 mmol) in dry dichloromethane (5 mL), 2-nitrobenzenesulfonylchloride (3.2 g, 14.37 mmol) in dichloromethane (8 mL) and triethylamine (2.6 mL, 17.97 mmol) at 0° C. were slowly added under nitrogen atmosphere. The solution was stirred for 2 hours. After completion of the reaction (indicated by TLC), water (10 mL) was added to the reaction mixture, then extracted with dichloromethane (2×15 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 60:40) to yield (S)-compound 5 as a pale yellow oil (2.33 g, 75%); [α]22D=+80.2 (c 2.1, CHCl3); IR (CHCl3): νmax 3546, 3367, 3022, 2883, 2401, 1594, 1542, 1412, 1362, 1216, 1170, 1125, 1059, 971, 854, 668 cm−1; 1H NMR (200 MHz, CDCl3): δH 1.13 (d, J=6.5 Hz, 3H), 2.16 (bs, 1H), 3.45-3.70 (m, 3H), 5.61 (d, J=6.6 Hz, 1H), 7.73-7.80 (m, 2H), 7.86-7.91 (m, 1H), 8.13-8.22 (m, 1H); 13C NMR (50 MHz, CDCl3): δC 147.8 (C), 134.4 (C), 133.7 (CH), 133.0 (CH), 130.9 (CH), 125.5 (CH), 66.2 (CH2), 52.5 (CH), 17.8 (CH3); MS: m/z 283 [M+Na]+.
To a stirred solution of triphenyl phosphine (4.15 g, 15.85 mmol), imidazole (1.1 g, 15.85 mmol) in dry dichloromethane (20 mL), iodine (4.8 g, 19.02 mmol) at 0° C. was added and the solution was stirred for 5 min. To this, 3-fluoro benzyl alcohol (compound 6) (2 g, 15.85 mmol) dissolved in dichloromethane (5 mL) was added drop wise over 10 min and the stirring was continued for 1 hour with exclusion of light. After completion of the reaction (indicated by TLC), the reaction mixture was quenched by addition of an aqueous Na2S2O3 solution (15 mL), then extracted with dichloromethane (2×20 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 95:5) to yield compound 7 as a colorless oil (3.5 g, 95%); (IR (CHCl3): νmax 3460, 3060, 2965, 1695, 1613, 1593, 1482, 1446, 1259, 1156, 1068, 944, 871, 782, 736, 686 cm−1; 1H NMR (200 MHz, CDCl3): δH 4.42 (s, 2H), 6.89-6.99 (m, 1H), 7.05-7.17 (m, 2H), 7.21-7.29 (m, 1H); 13C NMR (50 MHz, CDCl3): δC 165.0 (C), 141.6 (C), 130.2 (CH), 124.4 (CH), 115.9 (CH), 114.7 (CH), 3.9 (CH2).
To a stirred solution of 4-(hydroxymethyl)phenol (1.57 g, 12.7 mmol) and K2CO3 (8.8 g, 63.55 mmol) in dry acetonitrile (25 mL), compound 7 (3 g, 12.7 mmol) in acetonitrile was slowly added and the reaction mixture was heated at 70° C. for 6 hours. After completion of the reaction (indicated by TLC), water (20 mL) was added to the reaction mixture, then extracted with ethylacetate (3×20 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 70:30) to yield compound 8 as a colorless solid (2.7 g, 91%); mp 63-65° C.; IR (CHCl3): νmax 3422, 3017, 1612, 1512, 1489, 1381, 1216, 1174, 1020, 829, 668 cm−1; 1H NMR (200 MHz, CDCl3): δH 4.61 (s, 2H), 5.06 (s, 2H), 6.91-6.98 (m, 2H), 7.00-7.06 (m, 1H), 7.12-7.20 (m, 2H), 7.25-7.37 (m, 3H); 13C NMR (50 MHz, CDCl3): δC 165.4 (C), 160.5 (C), 158.0 (C), 139.6 (C), 133.5 (CH), 130.2 (CH), 128.7 (CH, 2 carbons), 122.7 (CH), 114.8 (CH, 2 carbons), 113.9 (CH), 69.1 (CH2), 64.9 (CH2); MS: m/z 255 [M+Na]+.
To a stirred solution of triphenyl phosphine (2.82 g, 10.8 mmol), imidazole (0.73 g, 10.76 mmol) in dry dichloromethane (20 mL), iodine (3.27 g, 12.9 mmol) at 0° C. was added and the solution was stirred for 5 min. To this, compound 8 (2.5 g, 10.8 mmol) dissolved in dichloromethane (5 mL) was added drop wise over 10 min and the stirring was continued for 1 hour with exclusion of light. After completion of the reaction (indicated by TLC), the reaction mixture was quenched by addition of an aqueous Na2S2O3 solution (15 mL), then extracted with dichloromethane (2×20 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 95:5) to yield compound 9 as a colorless oil (3.4 g, 93%); IR (CHCl3): νmax 3503, 3033, 2925, 2089, 1607, 1509, 1488, 1381, 1301, 1250, 1155, 1079, 944, 869, 776, 684 cm−1; 1H NMR (200 MHz, CDCl3): δH 4.47 (s, 2H), 5.04 (s, 2H), 6.85-6.91 (m, 2H), 6.96-7.02 (m, 1H), 7.05-7.12 (m, 1H), 7.16-7.20 (m, 1H), 7.29-7.40 (m, 3H).
13C NMR (50 MHz, CDCl3): δC 165.4 (C), 160.5 (C), 158.1 (C), 131.9 (C), 130.2 (CH), 130.1 (CH, 2 carbons), 122.7 (CH), 115.1 (CH, 2 carbons), 114.7 (CH), 113.9 (CH), 69.2 (CH2), 6.33 (CH2).
To a stirred solution of (S)-compound 5 (1 g, 3.8 mmol) and K2CO3 (2.65 g, 19.2 mmol) in dry acetonitrile (25 mL), compound 9 (1.84 g, 5.4 mmol) in acetonitrile was slowly added and the reaction mixture was heated at 70° C. for 72 hours. After completion of the reaction (indicated by TLC), water (20 mL) was added to the reaction mixture, then extracted with ethylacetate (3×15 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 80:20) to yield (S)-compound 10 as a colorless oil (1.46 g, 80%); [α]22D=+5.4 (c 1.5, CHCl3); IR (CHCl3): νmax 3445, 3020, 2928, 2400, 1613, 1544, 1512, 1453, 1371, 1216, 1162, 1029, 852, 668 cm−1; NMR (200 MHz, CDCl3): δH 1.07 (d, J=6.9 Hz, 3H), 1.91 (t, J=5.2 Hz, 1H), 3.41-3.53 (m, 2H), 4.05-4.22 (m, 1H), 4.37-4.57 (m, 2H), 5.02 (m, 2H), 6.87 (d, J=8.53 Hz, 2H), 6.97-7.12 (m, 2H), 7.20 (d, J=7.2 Hz, 2H), 7.32 (d, J=8.7 Hz, 2H), 7.47-7.67 (m, 3H), 7.89 (d, J=8.09 Hz, 1H); 13C NMR (50 MHz, CDCl3): δC 165.5 (C), 160.6 (C), 158.4 (C), 147.7 (C), 139.6 (C), 134.1 (C), 133.4 (CH), 131.6 (CH), 131.4 (CH), 1303 (CH), 129.7 (CH, 2 carbons), 124.1 (CH), 122.8 (CH), 115.1 (CH), 114.9 (CH, 2 carbons), 114.0 (CH), 69.2 (CH2), 64.3 (CH2), 56.2 (CH), 46.9 (CH2), 15.4 (CH3); MS: m/z 497 [M+Na]+.
A mixture of (S)-compound 10 (1.25 g, 2.6 mmol), TEMPO (0.028 g, 0.18 mmol), acetonitrile (20 mL), and sodium phosphate buffer (16 mL, 0.67 M, pH 6.7) was heated to 35° C. Next, sodium chlorite (0.47 g dissolved in 2 mL water, 7.9 mmol) and diluted bleach (4-6%, 0.09 mL diluted in 1 mL water) were added simultaneously over 1 hour. The reaction mixture was stirred at 35° C. until the reaction was complete (3 hours, TLC), then cooled to room temperature. Water (30 mL) was added and the pH adjusted to 8 with 2 M NaOH. The reaction was quenched by pouring it into ice cold Na2SO3 solution maintained at <20° C. After stirring for 30 min at room temperature, ethyl acetate (20 mL) was added and the stirring was continued for an additional 15 min. The organic layer was separated and discarded. More ethyl acetate (20 mL) was added, and the aqueous layer was acidified with 1 M HCl to pH 3-4. The organic layer was separated, washed with water (2×15 mL), brine and concentrated under reduced pressure to afford the carboxylic acid (S)-compound 11 (1.1 g, 85%); [α]22D=−20.4 (c 1.1, CHCl3); IR (CHCl3): νmax 3398, 3095, 1718, 1612, 1591, 1543, 1512, 1489, 1457, 1371, 1303, 1251, 1163, 1059, 900, 852, 831, 778, 684 cm−1; 1H NMR (200 MHz, CDCl3): δH 1.44 (d, J=7.3 Hz, 3H), 4.23 (d, J=15.6 Hz, 1H), 4.64 (d, J=15.6 Hz, 1H), 4.82-4.90 (q, J=7.4 Hz, 1H), 4.92 (s, 2H), 6.68 (d, J=8.6 Hz, 2H), 6.89-7.01 (m, 2H), 7.07-7.13 (m, 3H), 7.18-7.33 (m, 2H), 7.43-7.55 (m, 3H), 8.81 (bs, 1H); 13C NMR (50 MHz, CDCl3): δC 176.5 (CO), 165.0 (C), 158.0 (C), 147.4 (C), 139.4 (C), 134.1 (C), 133.2 (CH), 131.4 (CH), 130.3 (CH), 129.9 (CH, 2 carbons), 128.4 (C), 124.1 (CH), 122.6 (CH), 115.0 (CH), 114.6 (CH, 2 carbons), 114.3 (CH), 113.8 (CH) 69.1 (CH2), 56.1 (CH), 49.0 (CH2), 16.8 (CH3); MS: m/z 511 [M+Na]+.
To a solution of carboxylic acid (S)-compound 11 (1 g, 2.04 mmol) and triethyl amine (0.34 mL, 2.4 mmol) in dry THF (20 mL), ethyl chloroformate (0.21 mL, 2.2 mmol) at 0° C. was added under nitrogen atmosphere. After 1 hour, ammonium hydroxide (25% w/v aqueous solution, 1.4 mL, 10.2 mmol) was added and the resulting reaction mixture was stirred at room temperature for 16 hours. After completion of the reaction, potassium carbonate (0.29 g, 2.1 mmol) was added and the reaction mixture was filtered, and washed with ethylacetate. The solvent was removed under reduced pressure and the crude product was subjected to column chromatography (silica gel, petroleum ether/EtOAc, 50:50) to obtain sulfonamide (S)-compound 12 as a colorless oil (0.9 g, 91%); [α]22D=−32.1 (c 1.2, CHCl3); IR (CHCl3): νmax 3472, 1961, 1611, 1592, 1542, 1511, 1449, 1371, 1304, 1243, 1163, 1060, 1029, 895, 852, 684 cm−1; 1H NMR (200 MHz, CDCl3): δH 1.43 (d, J=7.1 Hz, 3H), 4.44 (d, J=15.4 Hz, 1H), 4.59 (d, J=15.5 Hz, 1H), 4.60-4.71 (q, J=7.0 Hz, 1H), 5.01 (s, 2H), 5.50 (bs, 1H), 6.31 (bs, 1H), 6.78 (d, J=8.71 Hz, 2H), 6.98-7.11 (m, 2H), 7.15-7.22 (m, 3H), 7.31-7.45 (m, 2H), 7.59-7.64 (m, 3H); 13C NMR (50 MHz, CDCl3): δC 172.3 (CO), 165.5 (C), 158.2 (C), 147.5 (C), 139.6 (C), 139.4 (C), 133.6 (CH), 131.7 (CH), 130.5 (CH, 2 carbons), 130.3 (CH), 128.1 (C), 124.2 (CH), 122.7 (CH), 115.1 (CH), 114.7 (CH, 2 carbons), 114.4 (CH), 113.9 (CH), 69.0 (CH2), 55.7 (CH), 48.3 (CH2), 14.9 (CH3); MS: m/z 510 [M+Na]+.
To a solution of sulfonamide (S)-compound 12 (0.8 g, 1.64 mmol), potassium carbonate (0.56 g, 4.9 mmol) in dry DMF (10 mL), thiophenol (0.2 mL, 1.9 mmol) was added. The reaction mixture was vigorously stirred for 6 hours. After completion of the reaction (indicated by TLC), water (10 mL) was added to the reaction mixture, then extracted with ethylacetate (2×20 mL). The combined organic layers were washed with brine solution, dried over anhydrous Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude residue was done by column chromatography (silica gel, petroleum ether/EtOAc, 60:40) to yield (S)-compound of formula I as a colorless solid (0.43 g, 86%); mp 207-09° C.; [α]22D=+3.89 (c 1.55, CHCl3); IR (CHCl3): νmax 3341, 2970, 2927, 2853, 1648, 1592, 1512, 1489, 1445, 1406, 1384, 1254, 1176, 1137, 1030, 953, 928, 829, 680 cm−1; 1H NMR (200 MHz, CDCl3): δH 1.34 (d, J=6.9 Hz, 3H), 2.49 (bs, 2H), 3.19-3.30 (q, J=6.8 Hz, 1H), 3.63-3.78 (dd, J=19.4, 3.9 Hz, 2H), 5.05 (s, 2H), 5.85 (bs, 1H), 6.95 (d, J=8.7 Hz, 2H), 7.00-7.06 (m, 1H), 7.13-7.24 (m, 4H), 7.29-7.40 (m, 1H). 13C NMR (50 MHz, CDCl3): δC 178.3 (CO), 165.4 (C), 157.7 (C), 139.6 (C), 132.1 (C), 130.2 (CH), 129.3 (CH, 2 carbons), 122.7 (CH), 114.9 (CH, 2 carbons), 114.6 (CH), 113.9 (CH), 69.2 (CH2), 57.5 (CH), 51.9 (CH2), 19.6 (CH3); MS: m/z 302 [M]+, 325 [M+Na]+.
To a stirred solution of (S)-compound of formula I (0.1 g, 0.33 mmol) in ethylacetate (3 mL) at 70° C., methanesulfonic acid (0.02 mL, 0.33 mmol) was added and the reaction mixture was stirred for 2 hours. Subsequently, the temperature was lowered to 35° C. and the stirring was continued for additional 1 hour. The solvent was evaporated under reduced pressure and the residue was filtered through a short bed of basic alumina [eluent: EtOAc/MeOH; (95:5)] to obtain safinamide mesylate as a white solid (0.11 g, 90%); mp 209-10° C. [lit.7 mp 210]; [α]22D=+9.6 (c 1.1, AcOH); {lit.7 [α]22D=+12.9 (c 1.1, AcOH)} ee>98% [The ee of safinamide mesylate was determined by chiral HPLC analysis; Chiralcel OD-RH (150×4.6 mm) column; eluent: Methanol/Acetonitrile/Buffer-TEAP, pH 3 (20:10:70); flow rate 0.5 mL/min (780 psi); detector: 224 nm] [(R)-isomer tR=11.55 min, (S)-isomer tR=12.94 min].
The process of the present invention does not use any toxic reagents. Further, the process does not lead to formation of any toxic byproducts.
The compound of formula I obtained by the process of the present invention has enantiopurity greater than 98% (ee>98%).
The compound of formula I produced by the process of the present invention is used for treatment of Parkinson's disease, restless legs syndrome and epilepsy.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1307/DEL/2013 | May 2013 | IN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IN2014/000294 | 5/2/2014 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2014/178083 | 11/6/2014 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 20090149544 | Liu | Jun 2009 | A1 |
| 20130204042 | Sriram | Aug 2013 | A1 |
| Number | Date | Country |
|---|---|---|
| 2 474 521 | Jul 2012 | EP |
| WO 9803472 | Jan 1998 | WO |
| WO 2011047767 | Apr 2011 | WO |
| Entry |
|---|
| Muthukrishnan and Mujahid, “A New Enantioselective Synthesis of the Anti-Parkinson Agent Safinamide,” Synthesis (2014), 46:1751-1756, Georg Thieme Verlag, Stuttgart, New York. |
| Number | Date | Country | |
|---|---|---|---|
| 20160060211 A1 | Mar 2016 | US |
