Patent Grant
10100150
The present disclosure concerns embodiments of sequence-defined polymers and methods of making and using such sequence-defined polymers.
Sequence-defined polymers are epitomized in nature by polypeptides and poly(nucleic acids). In nature, these sequence-defined polymers can create biomaterials, encode information, perform biocatalysis, participate in molecular recognition, and shuttle species across membranes. Artificial sequence-defined polymers prepared by chemical synthesis likewise have the potential to create tremendous structural diversity and functionality based on monomer sequence, if such synthetic polymers can be developed.
To date the vast majority of synthetic sequence-defined polymers are polypeptides, pseudopeptides, and/or peptoids made in laboratories using in vitro application of macromolecular machines, solution chemical synthesis, or solid phase synthesis. Conventional sequence-defined polymers typically include amino acid structures with non-natural side chains and peptide bonds to achieve the reactivity associated with naturally-occurring polypeptides. A need exists in the art, however, for polymers that overcome shortcomings associated with conventional sequence-defined polymers, which are discussed herein. Methods for readily synthesizing such polymers also are needed, such as methods implementing readily available starting materials.
A need exists in the art for polymers that overcome the structural, and hence functional, limitations associated with current synthetically-accessible sequence-defined polymers. There exists a need in the art for more diverse polymer structures that can be readily synthesized, where a diversity of side chain structures can be incorporated onto the polymer chain, where the length of the polymer is defined, and where the sequence of monomers can be defined in a predetermined fashion. There is a need for such polymers such that the backbone and side chain structures may provide for intramolecular and/or intermolecular interactions that determine macromolecular conformation and/or self-assembly into materials. Discussed herein are novel polymer structures different from peptides, peptoids, poly(nucleic acids), or other conventional sequence-defined polymers, methods for readily synthesizing such polymers, and methods that can be implemented from readily available starting materials.
Disclosed herein are embodiments of polymer compounds. In some embodiments, the compounds can have a formula
wherein the variables of the formula are as recited herein. In some embodiments, when p is 1, the following can apply: each T and Tf is a terminal group independently selected from hydrogen, heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; R1, each Rn, and Rf independently is selected from hydrogen, heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; Y1, each Yp, and Yf independently is selected from a bond, —C(R4)2—, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl and wherein R4 of NR4 and at least one of R1 or Rn are different; W1 and each Wa independently is selected from —C(R4)2, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; Z1 and each Zb independently is selected from heteroaryl, oxygen, sulfur, or NR4, wherein R4 is selected from hydrogen, aliphatic, cyclic heteroaliphatic comprising one or more heteroatoms selected from oxygen, sulfur, selenium, phosphorous, or oxidized forms thereof, acyclic heteroaliphatic, heteroaryl, or aryl; L1 and each Lq independently is selected from aliphatic, heteroaliphatic, aryl, heteroaryl, carbosilane, silane, siloxane, polycarbosilane, polysilane, or polysiloxane; m ranges from 2 to 1000.
In other embodiments when p is 0, the following can apply: each T and Tf is a terminal group independently selected from hydrogen, heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; at least one of R1, Rn, and Rf is hydrogen, and remaining R1, Rn, and Rf independently are selected from heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; at least one of Y1, Yp, and Yf independently is selected from a bond, oxygen, sulfur, or NR4, wherein R4 is hydrogen, and remaining Y1, Yp, and Yf are selected from a bond, —C(R4)2—, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; W1 and each Wa independently is selected from —C(R4)2, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; Z1 and each Zb independently is selected from heteroaryl, oxygen, sulfur, or NR4, wherein R4 is selected from hydrogen, aliphatic, heteroaliphatic, heteroaryl, or aryl; L1 and each Lq independently is selected from aliphatic, heteroaliphatic, aryl, heteroaryl, carbosilane, silane, siloxane, polycarbosilane, polysilane, or polysiloxane.
In yet additional embodiments, such as when p is 0, any one or more of the following can apply: any one or more of R1 or Rn are not hydrogen if Y1 or Yp is NR4 where R4 is hydrogen and any one or more of L1 or Lq is phenyl; and any one or more of R1 or Rn are not hydrogen if Y1 or Yp is NR4 where R4 is hydrogen; any one or more of W1 or Wa is 0; any one or more of L1 or Lq is —CH2—C(4-decyloxybenzyl)2-CH2—; and any one of Z1 or Zb is NH; and at least one of R1, Y1, W1, Z1, or L1 is different from at least one Rn, Yp, Wa, Zb, or Lq, respectively.
In some embodiments, the polymer is assembled in the form of a membrane, an amorphous particle, a crystal, or a nanorod wherein a backbone of the polymer interacts to create a columnar structure and side chains of the polymer project outward from the column. In yet additional embodiments, the polymers are assembled in the form of a nanorod wherein side chains of a polymer interacts with side chains of another polymer to form the nanorod. In yet additional embodiments, the plurality of polymers is monodisperse, sequence-defined, or a combination thereof.
Also disclosed herein are methods of making the polymers and co-polymers comprising the disclosed polymers and non-triazine based polymers.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
Disclosed herein are novel synthetic polymers with diverse side chain functional groups whose unique, defined structures lead to properties and functionality lending to their use in materials science, nanotechnology, separation science, membranes, molecular recognition, assays, chemical or biomolecular sensors, biomedical therapy, pharmaceuticals, catalysis, and information storage.
In some embodiments the polymers exhibit high thermostability, resistance to hydrolytic degradation, and/or resistance to enzymatic or biological degradation, in comparison to natural or conventional sequence-defined polymers. Deficiencies in these areas can limit the usefulness of such natural or conventional sequence-defined polymers, which have peptide or other bonds subject to hydrolytic cleavage with or without enzymatic catalysis. Conventional sequence-defined polymers include polypeptides, pseudopeptides, and/or peptoids, which typically include linkers and/or peptide bonds that can be cleaved by under high temperatures and/or by enzymatic action thereby resulting in polymer degradation.
In some embodiments the polymers comprise hydrogen bond accepting atoms (such as nitrogens in the polymer backbones), hydrogen bond donating groups (such as N—H groups in the polymer backbones), or aromatic rings in the polymer backbones. In some embodiments, the polymers comprise interactive functional groups in the side chains that can participate in intramolecular or intermolecular interactions between monomer units, such that the polymers can self-organize into particular conformations or self-assemble to form materials. These conformations and materials may include artificial alpha helices, artificial beta sheets, and/or particular spatial arrangements of pendant side chains. Polymers that self-organize into particular conformations may participate in molecular recognition, have therapeutic or other pharmacologic biomedical effects, be useful in assays, sensors or separations, target bacteria, kill pathogens, or perform catalysis. Polymers that self-assemble through intermolecular interactions may form membranes, nanosheets, crystals, nanocapsules, microcapsules, nanotubules, microtubules, liquid crystals, fibers, nanofibers, or composite materials that contain additional components such as ions, minerals, or molecules and macromolecules.
Also disclosed herein are embodiments of defined polymer structures, and methods of synthesizing them, wherein molecular precursors, submonomers, and/or molecular monomers are assembled together using inexpensive starting materials that can produce diverse polymer structures comprising monomer units having various pendant side chains or linker sections by adaptive, scalable synthesis methods. In contrast to the polymerization methods disclosed herein, conventional methods of making sequence-defined peptide polymers typically require one-by-one addition of natural or non-natural amino acid groups to supports using repetitive deprotection and addition steps, which requires using rigid reaction conditions and a pre-assembled library of amino acids, thereby inhibiting large-scale adaptations of these methods. As disclosed herein, the inventors have developed molecular precursors, submonomers, and molecular monomers, and methods of constructing polymers using these components that eliminate the need to use protection/deprotection steps, expensive reagents, and/or pre-made libraries of monomers.
Embodiments of the presently disclosed methods utilize the starting materials to provide a molecular architecture with multiple reactive sites that can be sequentially acted upon in a pre-determined, well-controlled manner to create molecular precursors or submonomers disclosed herein. In certain embodiments, one and only one of the original or non-functionalized reactive sites is reactive at a given temperature. Accordingly, the starting material is only functionalized at a given position and not at additional positions in the same reaction; these additional positions can then be altered or functionalized under different conditions with different reagents. This sequential reactivity provides an advancement over prior methods used to form conventional sequence-defined peptide polymers as it provides completely new polymeric architectures, as compared to conventional peptide, peptoid, or nucleic acid polymers.
At the same time, molecular precursors, submonomers, and monomers with diverse pendant side chains, or even different linker sections, can be prepared in a straightforward manner from readily available or synthesized starting materials. A new molecular precursor, submonomer, or monomer prepared by this chemistry is more easily synthesized than, for example, unnatural alpha amino acids, which are required in conventional peptide-like polymers.
In certain disclosed embodiments, polymer structures comprising at least three monomer units are made. In such embodiments, the polymer is of a certain defined length, and has a defined, predetermined sequence of structurally different monomer units, where the sequence of monomer units arises from a predetermined plan, rather than as a result of a random copolymerization reaction.
Different types of predetermined sequences of monomers are contemplated by the present disclosure. In some embodiments, a predetermined sequence may be free of, or have no repeating patterns that exist consistently throughout the entire polymer. Solely by way of example, a hexamer of the sequence ABCADAC or ABCDEF is an example of a predetermined sequence without a repeating pattern, wherein A, B, C, D, E, and F represent monomer units disclosed herein.
In other disclosed embodiments, the predetermined sequence of monomers may have monomers that are present in repeating patterns, such as (AB)n, or (ABC)n, or (ABCBA)n, but that are monodisperse, or substantially monodisperse. The repeating patterns (AB)n, or (ABC)n, or (ABCBA)n are solely presented as examples and are not intended to be limiting. Predetermined sequences with repeating patterns can be made using the methods disclosed herein, and in some embodiments the methods of making can be used to produce polymers having a defined length, thereby producing a monodisperse or substantially monodisperse polymer. Such sequence-defined polymers are distinguished from those produced using conventional methods of making, such as batch polymerizations. These conventional methods can be used to produce polymers having repeating patterns; however, polymers produced using such methods do not have a defined length, and thereby are polydisperse.
In some other embodiments, the predetermined sequence of monomers can comprise monomers in blocks, such as (A)n(B)n, or (A)n(B)n(A)n, or (A)n(B)n(C)n, which are solely presented as exemplary patterns and are not intended to limit the present disclosure. Such embodiments are referred to herein as block copolymers. These block copolymers have a defined length and therefore are monodisperse or substantially monodisperse. These sequence-defined block copolymers are distinguished from those in the art that are produced from batch polymerizations, as polymers produced from batch polymerizations do not produce block copolymers having a defined length, and thereby are polydisperse.
The structurally different monomer units of any of the above-mentioned polymers may be different, or “defined,” as a result of having different pendant side chains, different linker sections, or a combination thereof. The linker section itself may have substituents on it. In some embodiments, the pendant side chain may simply be a hydrogen atom.
In yet other embodiments, polymers having a certain defined length, and a defined sequence of identical monomer units are disclosed. Such polymers are referred to herein as homopolymers. The homopolymers disclosed herein are monodisperse or substantially monodisperse in contrast to polydisperse homopolymers produced from conventional batch polymerization reactions.
Also disclosed herein are methods for determining the ability of the novel polymers to conform and/or assemble into different configurations and materials. These methods can be used to facilitate particular desired monomer and/or polymer designs. For example, computer simulations can be used to determine the forces and/or interactions that govern folding and assembly of the polymers. These simulations demonstrate that the polymers may fold into conformations in solution that resemble protein three dimensional structure, such as alpha-helices or beta sheets. These simulations can be used to show how backbone-backbone interactions by hydrogen bonding and pi-pi interactions may contribute to folding into conformations or self-assembly into materials. The simulations also may be used as a preliminary step in determining the appropriate molecular fragments to combine to form the desired polymers as well as methods to identify particular polymer structures useful for obtaining a particular desired configuration and/or polymer material. Also disclosed are experiments in which polymers assemble from solution into solid phase materials, demonstrating the potential for self-assembly into materials.
The following explanations of terms are provided to better describe the present disclosure and to guide those of ordinary skill in the art in the practice of the present disclosure. As used herein, “comprising” means “including” and the singular forms “a” or “an” or “the” include plural references unless the context clearly dictates otherwise. The term “or” refers to a single element of stated alternative elements or a combination of two or more elements, unless the context clearly indicates otherwise.
Unless explained otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting, unless otherwise indicated. Other features of the disclosure are apparent from the following detailed description and the claims.
Unless otherwise indicated, all numbers expressing quantities of components, molecular weights, percentages, temperatures, times, and so forth, as used in the specification or claims are to be understood as being modified by the term “about.” Accordingly, unless otherwise indicated, implicitly or explicitly, the numerical parameters set forth are approximations that can depend on the desired properties sought and/or limits of detection under standard test conditions/methods. When directly and explicitly distinguishing embodiments from discussed prior art, the embodiment numbers are not approximates unless the word “about” is recited. Furthermore, not all alternatives recited herein are equivalents.
To facilitate review of the various embodiments of the disclosure, the following explanations of specific terms are provided:
Aliphatic: A hydrocarbon, or a radical thereof, having at least one carbon atom to 50 carbon atoms, such as one to 25 carbon atoms, or one to ten carbon atoms, and which includes alkanes (or alkyl), alkenes (or alkenyl), alkynes (or alkynyl), including cyclic versions thereof, and further including straight- and branched-chain arrangements, and all stereo and position isomers as well.
Aldehyde: RaC(O)H, wherein Ra is the atom of the formulas disclosed herein to which the aldehyde group is attached.
Alkyl: A saturated monovalent hydrocarbon having at least one carbon atom to 50 carbon atoms, such as one to 25 carbon atoms, or one to ten carbon atoms, wherein the saturated monovalent hydrocarbon can be derived from removing one hydrogen atom from one carbon atom of a parent compound (e.g., alkane). An alkyl group can be branched, straight-chain, or cyclic (e.g., cycloalkyl).
Alkenyl: An unsaturated monovalent hydrocarbon having at least two carbon atoms 50 carbon atoms, such as two to 25 carbon atoms, or two to ten carbon atoms, and at least one carbon-carbon double bond, wherein the unsaturated monovalent hydrocarbon can be derived from removing one hydrogen atom from one carbon atom of a parent alkene. An alkenyl group can be branched, straight-chain, cyclic (e.g., cylcoalkenyl), cis, or trans (e.g., E or Z).
Alkynyl: An unsaturated monovalent hydrocarbon having at least two carbon atoms 50 carbon atoms, such as two to 25 carbon atoms, or two to ten carbon atoms and at least one carbon-carbon triple bond, wherein the unsaturated monovalent hydrocarbon can be derived from removing one hydrogen atom from one carbon atom of a parent alkyne. An alkynyl group can be branched, straight-chain, or cyclic (e.g., cycloalkynyl).
Aliphatic-aryl: An aryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the aryl group is or becomes coupled through an aliphatic group.
Alkylaryl/Alkenylaryl/Alkynylaryl: An aryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the aryl group is or becomes coupled through an alkyl, alkenyl, or alkynyl group, respectively.
Aliphatic-heteroaryl: A heteroaryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the heteroaryl group is or becomes coupled through an aliphatic group.
Alkylheteroaryl/Alkenylheteroaryl/Alkynylheteroaryl: A heteroaryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the heteroaryl group is or becomes coupled through an alkyl, alkenyl, or alkynyl group, respectively.
Alkoxy: —O-alkyl, with exemplary embodiments including, but not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, t-butoxy, sec-butoxy, n-pentoxy.
Amide: RaC(O)NRbRc wherein Ra is the atom of the formulas disclosed herein to which the amide is attached, and each of Rb and Rc independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, heteroaryl, or any combination thereof.
Amine: RaNRbRc, wherein Ra is the atom of the formulas disclosed herein to which the amine is attached, and each of Rb and Rc independently is selected from hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl, heteroalkynyl, heteroaryl, and any combination thereof.
Aryl: An aromatic carbocyclic group comprising at least five carbon atoms to 15 carbon atoms, such as five to ten carbon atoms, having a single ring or multiple condensed rings, which condensed rings can or may not be aromatic provided that the point of attachment is through an atom of the aromatic carbocyclic group.
Carboxyl: RaC(O)ORb, wherein Ra is the atom of the formulas disclosed herein to which the carboxyl group is attached and wherein Rb is alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl, heteroalkynyl, heteroaryl, hydrogen, and any combination thereof.
Dye: A molecule or molecular substituent with an absorption in the UV, visible or NIR spectrum, and may have an emission in the UV, visible or NIR. A dye is typically an aliphatic, aromatic, heteroaliphatic, or heteroaromatic molecule or molecular substituent.
Haloaliphatic: An aliphatic group wherein one or more hydrogen atoms, such as one to 10 hydrogen atoms, independently is replaced with a halogen atom, such as fluoro, bromo, chloro, or iodo.
Haloalkyl: An alkyl group wherein one or more hydrogen atoms, such as one to 10 hydrogen atoms, independently is replaced with a halogen atom, such as fluoro, bromo, chloro, or iodo. In an independent embodiment, haloalkyl can be a CX3 group, wherein each X independently can be selected from fluoro, bromo, chloro, or iodo.
Heteroaliphatic: An aliphatic group comprising at least one heteroatom to 20 heteroatoms, such as one to 15 heteroatoms, or one to 5 heteroatoms, which can be selected from, but not limited to oxygen, nitrogen, sulfur, selenium, phosphorous, and oxidized forms thereof within the group.
Heteroalkyl/Heteroalkenyl/Heteroalkynyl: An alkyl, alkenyl, or alkynyl group (which can be branched, straight-chain, or cyclic) comprising at least one heteroatom to 20 heteroatoms, such as one to 15 heteroatoms, or one to 5 heteroatoms, which can be selected from, but not limited to oxygen, nitrogen, sulfur, selenium, phosphorous, and oxidized forms thereof within the group.
Heteraliphatic-aryl: An aryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the aryl group is or becomes coupled through a heteroaliphatic group.
Heteroalkylaryl/Heteroalkenylaryl/Heteroalkynylaryl: An aryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the aryl group is or becomes coupled through a heteroalkyl, heteroalkenyl, or heteroalkynyl group, respectively.
Heteroalkylheteroaryl/Heteroalkenylheteroaryl/Heteroalkynylheteroaryl: A heteroaryl group that is or can be coupled to a starting material, molecular precursor, submonomer, molecular monomer, or polymer embodiment disclosed herein, wherein the aryl group is or becomes coupled through a heteroalkyl, heteroalkenyl, or heteroalkynyl group, respectively.
Heteroaryl: An aryl group comprising at least one heteroatom to six heteroatoms, such as one to four heteroatoms, which can be selected from, but not limited to oxygen, nitrogen, sulfur, selenium, phosphorous, and oxidized forms thereof within the ring. Such heteroaryl groups can have a single ring or multiple condensed rings, wherein the condensed rings may or may not be aromatic and/or contain a heteroatom, provided that the point of attachment is through an atom of the aromatic heteroaryl group.
Heteropolymer: A polymer comprising multiple different monomer units, wherein the multiple different monomer units are be distinguished by a side chain, a linker group, a terminal group, or a combination thereof and at least two monomer units are not the same. In particular disclosed embodiments, a heteropolymer is linear and is not, and does not constitute, a dendrimer, such as a triazine-containing dendrimer. Such dendrimers consist of multiple branches, rather than just one branching point.
Homopolymer: A polymer comprising identical monomer units and that is monodisperse or substantially monodisperse. In independent embodiments, the homopolymer is linear and is not, and does not constitute, a dendrimer, such as a triazine-containing dendrimer. Such dendrimers consist of multiple branches, rather than just one branching point.
Ketone: RaC(O)Rb, wherein Ra is the atom of the formulas disclosed herein to which the ketone is attached, and Rb is selected from aliphatic, heteroaliphatic, aryl, heteroaryl, and any combination thereof.
Molecular Precursor: In some embodiments, this term encompasses a functionalized starting material wherein at least one of the reactive positions of the starting material has been functionalized with a side chain, linker group, or a terminal group and a third reactive position comprises a reactive group. In particular disclosed embodiments, a molecular precursor can be a functionalized starting material wherein at least two of the reactive positions of the starting material have each been functionalized with a side chain, linker group, or a terminal group and a third reactive position comprises a reactive group.
Monodisperse: A monodisperse polymer is a polymer as contemplated by the present disclosure wherein the length (or number of monomer units) obtained in a final polymer product is defined by a specific number of starting materials, molecular precursors, or submonomers attached during synthesis of the polymer and substantially all the polymer chains (e.g., at least 80% to 99% of the polymer chains, such as at least 80%, 85%, 90%, 95%, or 99% of the polymer chains) are of the same length defined by the synthesis. As an example, in a method embodiment wherein 6 molecular precursors (or 6 submonomers, or 6 starting material units) are combined to form a polymer, at least 80% of the polymer chains formed in the method will consist of 6 monomer units, with particular embodiments having at least 90% of the polymer chains consisting of exactly 6 monomer units.
Monomer Unit: A moiety present in a polymer that comprises a starting material that has been functionalized at all three reactive positions. Some monomer units can be external monomer units, which comprise (a) a side chain group, (b) a terminal group, and (a) a linker group, wherein the terminal group constitutes one end of the polymer and the linker group couples the monomer unit to another monomer unit. Some monomer units can be internal monomer units, which comprise (a) a side chain group and (b) at least one linker group, wherein the linker group couples the monomer unit to at least one other monomer unit (a terminal monomer unit, an internal monomer unit, two terminal monomer units, two internal monomer units, or a combination of a terminal monomer unit and an internal monomer unit).
Non-triazine based polymer: A polymer comprised of monomers units, where the monomer units are not comprised of triazine rings in the backbone of the polymer, including such polymers as are known in the art. Examples include, but are not limited to, a polynucleic acid; a peptide; a peptoid; a pseudopeptide polymer; a polyalkylene oxide, such as a poly(ethylene glycol) (e.g., PEG); a polyester (e.g., poly(ethylene terephthalate, a polylactide, a polyglycolide, or copolymers thereof); a polyamide (e.g., nylon, polyphthalamides formed from hexamethylenediamine and terephthalic acid, or aramides formed from paraphenylenediamine and terephthalic acid); a poly(alkene) (e.g., polyethylene, polypropylene, polymethylpentene, or polybutene-1, polybutadiene, polystryrene); a polysiloxane; or a polycarbosilane.
Peptide: A peptide is a polymer consisting of amino acid monomers linked together by amide bonds, where the amino acids are glycine or substituted glycine monomers, where substituents are attached to the alpha-carbon of the substituted glycine. Peptide includes oligopeptides and polypeptides.
Peptoid: A peptoid is a polymer consisting of N-substituted glycine monomers, e.g., poly-N-substituted glycines, where the substituents on the glycine monomers are side-chains. These are contrasted with typical peptides where substituent side chains are attached to the alpha-carbon of the amino acid monomers, which are substituted glycine monomers.
Pseudopeptide polymer: A pseudopepide polymer is a polymer comprising nonnatural amino acids, linked together by peptide bonds.
Sequence-Defined Polymer: A heteropolymer comprising at least three monomers, wherein each monomer can have a different side chain, linker group, and/or terminal group, and wherein the monomers are arranged in a pre-determined specific sequence, with the pre-determined specific sequence being determined prior to synthesis of the heteropolymer.
Solid Support: A solid, inorganic or organic material that is appended to one end of a molecular precursor, monomer, or polymer disclosed herein and that typically is inert to the reaction conditions used to form a polymer chain thereby allowing the solid support to remain attached to the growing polymer chain. The solid support can be selectively cleaved to free the polymer.
Submonomer: This term refers to a functionalized starting material functionalized with a side chain, a linker group, or a terminal group, and further comprising at least two reactive groups that can each be replaced with a linker, a terminal group, or a side chain. This term also can be used to refer to the linker, terminal group, or side chain that is used to replace one or more of the two reactive groups of the functionalized starting material.
Sulfonyl/Sulfonate: A functional group having a formula RaSO2Rb, wherein Ra is the atom of the formulas disclosed herein to which the sulfonyl or sulfonate is attached, and Rb is selected from hydrogen, aliphatic, heteroaliphatic, aryl, heteroaryl, and any combination thereof.
Transition metal complex. A transition metal complex is a coordination compound comprising a metal atom or ion with one or more ligands.
A person of ordinary skill in the art would recognize that the definitions provided above are not intended to include impermissible substitution patterns (e.g., methyl substituted with 5 different groups, and the like). Such impermissible substitution patterns are easily recognized by a person of ordinary skill in the art. Any functional group disclosed herein and/or defined above can be substituted or unsubstituted, unless otherwise indicated herein. Also, in particular embodiments, the use of the terms “(hetero)alkyl,” “(hetero)alkenyl,” “(hetero)alkynyl,” “(hetero)aryl,” “(hetero)alkyl(hetero)aryl,” “(hetero)alkenyl(hetero)aryl,” and “(hetero)alkynyl(hetero)aryl” can be used to indicate that the functional group can be a heteroatom-containing or a non-heteroatom-containing version of the particular designated alkyl, alkenyl, alkynyl, or aryl group. For example, “(hetero)alkyl” can encompass “heteroalkyl” or “alkyl” unless otherwise specifically indicated.
Disclosed herein are embodiments of molecular precursors and submonomers that can be used to make polymers, such as heteropolymers and homopolymers. The diverse selection of molecular precursors and/or submonomers that can comprise various different types of functionalized groups provides the ability to obtain defined polymers with monomer units having specific pendant side chains, linker groups, and/or terminal groups. Such polymers can be “defined,” wherein the polymer is defined by a pre-determined sequence of different monomer units. In some embodiments, the pendant side chain group or the linker groups can be selected and used to define the polymer sequence. The polymers described herein exhibit superior stability in comparison to sequence-defined peptide polymers, which are defined by the side chains of amino acid monomers. Without being bound to a particular theory, it is currently believed that the lack of hydrolysable peptide bonds in the polymer chain can contribute the stability of the polymers disclosed herein.
The disclosed polymers also have substantially different structures from sequence peptide polymers, thereby providing the ability to produce functional macromolecules and materials for the applications described herein. In some embodiments, the polymers can be assembled to have a specific pre-determined number of monomer units, and hence a defined length, thereby contributing to the monodispersity of particular polymers disclosed herein. Such monodispersity confers to the polymers the ability to assemble into materials with ordered structures.
The polymers disclosed herein can have the type formula illustrated below in Formula 1.
With respect to Formula 1, the polymer comprises terminal groups, which can be the same or different and attached to each terminus of the polymer. Terminal groups can be an atom or group of atoms (such as a functional group), or they can include atoms that tether the polymer to a solid phase support. In some embodiments, one or more terminal groups can be a solid phase support.
The polymers also can comprise monomer units having side chains, which can be the same or different. A side chain can be an atom or a group of atoms, which can comprise one or more functional groups. As indicated previously, the side chains can be used to define the polymeric sequence.
The polymers also comprise linkers, which may be the same or different. A linker can be an atom or group of atoms (such as a functional group) that joins the consecutive monomers having a core ring A in the polymer. The linker can be derived from any suitable molecule or reagent that can be used to functionalize a starting material having a core ring A, and can subsequently bond to an additional submonomer or molecular precursor in polymer synthesis.
Core ring A can be a 5-membered or 6-membered ring, such as a 5-membered aromatic or 6-membered aromatic ring system. Core ring A can comprise one or more heteroatoms, with particular embodiments comprising three nitrogen atoms.
In some embodiments, the terminal group can be a submonomer that is structurally related to the monomer units in the polymer chain, for example, a submonomer comprising a core ring A without a linker. In such embodiments, the core ring A in the final submonomer may have a side chain and may have an additional group serving as a terminal group to the submonomer.
In some embodiments, a terminal group may be another type of polymer, or a linker section may be another type of polymer. In some embodiments, a terminal group may be a dye, or a linker section may be a dye.
In particular disclosed embodiments, the polymers can have any one of the formulas illustrated below.
In some embodiments, the polymer of Formula 2B can be substantially similar to or identical to Formula 2B, wherein the Te group of Formula 2A is a triazinyl group shown explicitly in Formula 2C, and the triazinyl group is a submonomer. In all formulas disclosed herein, it is understood that Te can be submonomers terminated with a final Tf group as illustrated in, for example, Formulas 2A and 2C.
In some embodiments, the monomer units of the polymers satisfying Formulas 2A-2C can have any of the types of predetermined sequences disclosed herein, such as a non-repeating unit sequence, a repeating unit sequence, a block copolymer sequence, or a homopolymer sequence. In particular disclosed embodiments, polymers satisfying Formulas 2A-2C are sequence-defined polymers that are heteropolymers having a defined length and comprising multiple different monomer units arranged in a specific predetermined sequence. Each monomer of the polymer unit can be distinguished by having different side chains pendant to the polymer chain. In other embodiments, each monomer unit of the polymer can be distinguished by having different sequences of atoms in the linker section between the triazine rings in the polymer backbone. In some other embodiments, monomer units of the polymer can be distinguished by a terminal group. Sequence-defined polymers comprise at least three monomer units, wherein each monomer unit can have a different side chain group, linker section, and/or terminal group and wherein the monomer units are arranged in a pre-determined sequence, and are not arranged randomly. These polymers are distinguished from a polymer where the monomers are arranged randomly resulting from a batch polymerization of a mixture of monomers.
Other exemplary polymers disclosed herein can have a formula according to any of those illustrated below. The polymers illustrated in Formulas 3A, 3B, 4A, and 4B have repeating patterns of the form (AB)n and (ABC)n respectively. These polymers are provided solely as illustrative examples as other repeating patterns can be used. The methods of making polymers, disclosed herein, can make sequences that repeat in this way while also defining the length of the polymer, in contrast to sequence controlled polymers that have repeating sequences of this type but whose lengths are not defined.
In some embodiments, the polymers can satisfy Formulas 5A, 5B, 6A and 6B. Such polymers are block copolymers have repeating patterns, such as in the form of (A)n(B)m and (A)n(B)m(C)p, respectively. Again, these examples are provided for illustrative purposes. The methods of making polymers, disclosed herein, can make sequences that repeat in this way while also defining the length of the polymer, in contrast to block copolymers that have repeating sequences of this type but whose lengths are not defined.
In some embodiments, the polymers disclosed herein can be homopolymers that satisfy Formulas 7A and/or 7B illustrated below. The methods of making polymers, disclosed herein, can be used to make these homopolymers while also defining the length of the polymer, so that it is monodisperse or substantially monodisperse, in contrast to polydisperse homopolymers, which are obtained from conventional batch polymerizations.
With reference to any one of the formulas provided above, the “R—Y” groups are side chains that are pendant to the overall polymer backbone. The polymer backbone includes a core with an architecture around which the other components are assembled. In some embodiments, this core, as illustrated in the above formulas, can be a triazine ring. In an independent embodiment, the core can be a pyridine or diazine ring system. The core is part of the starting material, which is described in more detail below. The side chains are attached to this core through covalent linkages. Also illustrated in the above formulas are “W-L-Z” sections that represent linker sections between the cores of sequential monomers or repeat units. The “T” group illustrated in the above formulas is a terminal group. Superscripts on the variables of the above formulas indicate that the atoms and structures of these variables can vary from one monomer to another, such as in a heteropolymer (e.g., a sequence-defined polymer).
In particular embodiments, the variables of the above formulas can be selected from any combination of the following:
T can be a terminal group as disclosed above which may or may not be bound to a solid support;
Te can be a terminal group as disclosed above, a submonomer, a linker group, or any other atomic, molecular or macromolecular structure that can be substituted onto the core of the final core that becomes the final monomer unit;
Tf can be a terminal group as disclosed above, comprising a submonomer, or comprising any other atomic, molecular or macromolecular structure that can be substituted onto the core of the final core that becomes the final monomer unit;
each of R1, R2, R3, Rn, and Rf independently can be selected from hydrogen, aliphatic, heteroaliphatic, aryl, heteroaryl, aliphatic-aryl, haloaliphatic, and heteroaliphatic-aryl;
each of Y1, Y2, Y3, Yp, and Yf independently can be selected from a bond, aliphatic, oxygen, sulfur, a bond, and NR4, wherein R4 is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl;
each of W1, W2, W3, and Wa independently can be selected from aliphatic, oxygen, sulfur, and NR4;
each of Z1, Z2, Z3, and Zb independently can be selected from oxygen, aliphatic, sulfur, NR4, and heteroaryl;
each of L1, L2, L3, or Lq can be selected from aliphatic, heteroaliphatic, aryl, heteroaryl, carbosilane (or polycarbosilane), silane (or polysilane), siloxane (or polysiloxane), an amino acid, a peptide, a pseudopeptide polymer, a peptoid, transition metal complex (e.g., a complex comprising a transition metal and a porphyrin or a phthalocyanine compound), or a polynucleic acid, a polyalkylene oxide (such as a poly(ethylene glycol) (e.g., PEG)), a polyester (e.g., poly(ethylene terephthalate, a polylactide, a polyglycolide, or copolymers thereof), a polyamide (e.g., nylon, polyphthalamides formed from hexamethylenediamine and terephthalic acid, or aramides formed from paraphenylenediamine and terephthalic acid), or a poly(alkene) (e.g., polyethylene, polypropylene, polymethylpentene, polybutene-1, polybutadiene, or polystyrene);
each n′ and p′ independently can range from 1 to 1000;
p can be 0 or 1; and
each m can be 2 to 1000.
With reference to Formula 2A, in some embodiments p is 1 and the following apply: each T and Tf is a terminal group independently selected from hydrogen, heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; R1, each Rn, and Rf independently is selected from hydrogen, heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; Y1, each Yp, and Yf independently is selected from a bond, —C(R4)2—, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl and wherein R4 of NR4 and at least one of R1 or Rn are different; W1 and each Wa independently is selected from —C(R4)2, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; Z1 and each Zb independently is selected from heteroaryl, oxygen, sulfur, or NR4, wherein R4 is selected from hydrogen, aliphatic, cyclic heteroaliphatic comprising one or more heteroatoms selected from oxygen, sulfur, selenium, phosphorous, or oxidized forms thereof, acyclic heteroaliphatic, heteroaryl, or aryl; L1 and each Lq independently is selected from aliphatic, heteroaliphatic, aryl, heteroaryl, carbosilane, silane, siloxane, polycarbosilane, polysilane, or polysiloxane; and m ranges from 2 to 1000.
With reference to Formula 2A, in some embodiments, p is 0 and the following apply: each T and Tf is a terminal group independently selected from hydrogen, heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; at least one of R1, Rn, and Rf is hydrogen, and remaining R1, Rn, and Rf independently are selected from heteroaliphatic, aliphatic, aryl, heteroaryl, aliphatic-aryl, aliphatic-heteroaryl, heteroaliphatic-aryl, or heteroaliphatic-heteroaryl; at least one of Y1, Yp, and Yf independently is selected from a bond, oxygen, sulfur, or NR4, wherein R4 is hydrogen, and remaining Y1, Yp, and Yf are selected from a bond, —C(R4)2—, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; W1 and each Wa independently is selected from —C(R4)2, oxygen, sulfur, or NR4, wherein each R4 independently is selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; Z1 and each Zb independently is selected from heteroaryl, oxygen, sulfur, or NR4, wherein R4 is selected from hydrogen, aliphatic, heteroaliphatic, heteroaryl, or aryl; L1 and each Lq independently is selected from aliphatic, heteroaliphatic, aryl, heteroaryl, carbosilane, silane, siloxane, polycarbosilane, polysilane, or polysiloxane; and provided that, when p is 0, any one or more of R1 or Rn are not hydrogen if Y1 or Yp is NR4 where R4 is hydrogen and any one or more of L1 or Lq is phenyl; and any one or more of R1 or Rn are not hydrogen if Y1 or Yp is NR4 where R4 is hydrogen; any one or more of W1 or Wa is 0; any one or more of L1 or Lq is —CH2—C(4-decyloxybenzyl)2-CH2—; and any one of Z1 or Zb is NH; and at least one of R1, Y1, W1, Z1, or L1 is different from at least one Rn, Yp, Wa, Zb, or Lq, respectively.
In particular disclosed embodiments of the formula illustrated above any combination of the following can be used:
each of R1, R2, R3, Rn, and Rf independently can be selected from hydrogen, (hetero)alkyl, (hetero)alkenyl, (hetero)alkynyl, (hetero)aryl, (hetero)alkyl(hetero)aryl, (hetero)alkenyl(hetero)aryl, haloalkyl, and (hetero)alkynyl(hetero)aryl);
each of Z1, Z2, Z3, and Zb independently can be a triazole;
each of L1, L2, or Lq can have a formula of —[C(R5)3]1-10—(X)0-1—[C(R5)3]1-10—, wherein each R5 independently can be selected from hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl, and X is selected from oxygen, sulfur, or NR4; or each of L1, L2, or Lq can be selected from a polymer known in the art such as a polynucleic acid, a peptide, a peptoid, a pseudopeptide polymer, a polyalkylene oxide (such as a poly(ethylene glycol) (e.g., PEG)), a polyester (e.g., poly(ethylene terephthalate, a polylactide, a polyglycolide, or copolymers thereof), a polyamide (e.g., nylon, polyphthalamides formed from hexamethylenediamine and terephthalic acid, or aramides formed from paraphenylenediamine and terephthalic acid), or a poly(alkene) (e.g., polyethylene, polypropylene, polymethylpentene, polybutene-1, polybutadiene, or polystyrene), a polysiloxane, a polycarbosilane; or each of L1, L2, or Lq can be selected from a transition metal complex comprising a transition metal and a porphyrin or a phthalocyanine;
each n′ and p′ independently can range from 2 to 100, or 2 to 10;
each m can range from 2 to 100, or 2 to 10;
T can be selected from (hetero)alkyl, (hetero)alkenyl, (hetero)alkynyl, (hetero)aryl, (hetero)alkyl(hetero)aryl, (hetero)alkenyl(hetero)aryl, and (hetero)alkynyl(hetero)aryl);
Tf can be selected from hydrogen, —NH2, or any structure equivalent to Rn—Yp; and
Rf—Yf is selected from any structure equivalent to Rn—Yp.
In exemplary embodiments of the above formulas, any combination of the following can be used:
each of R1, R2, R3, Rn, and Rf independently can be selected from C1-25alkyl, C2-25alkenyl, C2-25alkynyl, C1-25heteroalkyl (e.g., C2-25alkoxy, C1-25hydroxyl, C2-25thioether, C1-25thiol, C1-25amine, C1-25haloalkyl), C2-25heteroalkenyl (e.g., C3-25alkoxy, C2-25hydroxyl, C3-25thioether, C2-25thiol, C2-25amine, C2-25haloalkenyl), C2-25heteroalkynyl (e.g., C3-25alkoxy, C2-25hydroxyl, C3-25thioether, C2-25thiol, C2-25amine, C2-25haloalkynyl), C6-15aryl (e.g., phenyl or naphthyl, wherein either may be meta-, ortho-, and/or para-substituted with one or more hydroxyl, amine, carboxyl, halogen, alkyl, alkenyl, alkynyl, alkoxy, thioether, ketone, or ester), or C2-14heteroaryl (e.g., pyridinyl, pyrimidinyl, pyrrolyl, furanyl, or thiopheneyl); CX3, wherein each X independently can be selected from Cl, F, Br, or I;
each L1, L2, L3, or Lq can be selected from C1-10alkyl, such as C1-5alkyl; and
T or Te can have a formula (R4)p″—[Z(CH2)n″Z]m′—, wherein n″ and m′ independently can range from 1 to 20, R4 is as recited above, p″ is 1 or 2, and each Z independently can be O, NR4, or S). In some embodiments, the terminal group can be selected from an alkylene glycol (e.g., R4—[O(CH2)n″O]m′—, wherein n″ and m′ independently can range from 1 to 20 and R4 is as recited above), alkylene amino (e.g., (R4)2—[N(CH2)n″NR4]m′—, wherein n″ and m′ independently can range from 1 to 20 and each R4 independently can be as recited above), and alkylene thio (e.g., R4—[S(CH2)n″S]m′—, wherein n″ and m′ independently can range from 1 to 20 and each R4 is as recited above). In yet additional embodiments, T or Te can be selected from carboxyl, aldehyde, ester, amide, or amine. In some embodiments, T or Te may further comprise a solid support, such as a bead, a resin, or a combination thereof. For example, T or Te can be a solid support directly coupled to the monomer, or it can be a group as described above that is coupled to a solid support;
Yet other exemplary embodiments of the polymers can have a Formula 8, illustrated below.
With reference to Formulas 8A and 8B, each of T, Tf, R1, Rn, Rf, p, and m can be as recited above, and each R5 (when present) can be selected from aliphatic, heteroaliphatic, heteroaryl, or aryl, with particular embodiments being methyl and naphthyl. In exemplary embodiments, T can be an alkylene oxide group (e.g., ethylene glycol), and alkylene amino group (e.g., ethylene diamine, 1-methyl ethylene diamine), or an alkylene thio group (e.g., ethylene dithiol). In exemplary embodiments, each R5 (when present) independently can be selected from methyl, ethyl, propyl, butyl, or the like.
In particular disclosed embodiments, and with respect to any one of the formulas above, any one or more of the following can apply:
any one or more of R1, R2, R3, Rn, or Rf are not hydrogen if Y1, Y2, Y3, Yp, or Yf is NR4 where R4 is hydrogen and any one or more of L1, L2, L3, or Lq is phenyl;
any one or more of R1, R2, R3, Rn, or Rf are not hydrogen if Y1, Y2, Y3, Yp, or Yf is NR4 where R4 is hydrogen; any one or more of W1, W2, W3, or Wa is 0; any one or more of L1, L2, L3, or Lq is —CH2—C(4-decyloxybenzyl)2-CH2—; and any one of Z1, Z2, Z3, or Zb is NH; or
any one or more of R1, R2, R3, Rn, or Rf is not butyl if any one or more of Y1, Y2, Y3, Yp, or Yf is NR4 where R4 is butyl and any one or more of L1, L2, L3, or Lq is phenyl or -Ph-O-Ph-;
any one or more of L1, L2, L3, or Lq does not form a piperazine ring with W1, W2, W3, Wa, Z1, Z2, Z3, or Zb;
any one or more of R1, R2, R3, Rn, or Rf is not —(CH2)2[O(CH2)]3NH2 if Y1, Y2, Y3, Yp, or Yf is NR4 where R4 is hydrogen,
any one or more of L1, L2, L3, or Lq is not —C(O)— if Y1, Y2, Y3, Yp, or Yf is a bond and R1, R2, R3, Rn, or Rf is phenyl; or
the polymer is not a dendrimer.
Also disclosed herein are co-polymers comprising a first polymer of the co-polymer having a structure meeting a formula
and a second polymer coupled to the first polymer, wherein the second polymer is a polymer known in the art such as a polynucleic acid, a peptide, a peptoid, a pseudopeptide polymer, a polyalkylene oxide (such as a poly(ethylene glycol) (e.g., PEG)), a polyester (e.g., poly(ethylene terephthalate, a polylactide, a polyglycolide, or copolymers thereof), a polyamide (e.g., nylon, polyphthalamides formed from hexamethylenediamine and terephthalic acid, or aramides formed from paraphenylenediamine and terephthalic acid), a poly(alkene) (e.g., polyethylene, polypropylene, polymethylpentene, or polybutene-1, polybutadiene, or polystyrene), a polysiloxane, or a polycarbosilane. Such polymers known in the art, not containing a triazine ring in the backbone, are non-triazine based polymers. In particular disclosed embodiments, the co-polymers can have structures satisfying the formula:
wherein each A independently is a non-triazine based polymer and each p″ and s independently is 0 or 1; the remaining variables are as recited herein. Some exemplary co-polymers are illustrated below.
Exemplary polymer embodiments are illustrated below in Table 1. In some embodiments, the polymer is an exemplary sequence-defined polymer that is a heteropolymer of defined length comprising multiple different monomers arranged in a specific predetermined sequence. The monomers are distinguished in this case by having different side chains pendant to the polymer chain. The monomers are not present in any repeating pattern that exists within the entire sequence of the polymer.
In particular disclosed embodiments, starting materials can be used to make molecular precursors and/or submonomers that are used to make the monomer units of the polymers disclosed herein.
In particular disclosed embodiments, starting materials comprise a core with multiple reactive groups, which can be the same or different. Suitable starting materials have the property that reaction of one reactive group renders the remaining group or groups substantially less reactive. In this way, the starting material can be selectively be monosubstituted in a first reaction and then subsequently monosubstituted in a second reaction, and so on. The reaction can constitute using more rigorous conditions, such as a rise in temperature or use of a stronger reagent. More rigorous conditions are then required to act on the remaining reactive group or groups. Solely by way of example, a starting material comprising three reactive groups, such as multiple halogen atoms, can be selectively functionalized by first reacting one of the halogen atoms at a temperature ranging from −25° C. to 5° C. Upon raising the temperature to 18° C. to 40° C., a second halogen atom can be displaced. Temperatures greater than 70° C. (e.g., 70° C. to 140° C., 70° C. to 120° C., or 70° C. to 100° C.) can then be used to substitute a third halogen atom with a nucleophile. These temperatures ranges are exemplary and can vary with the particular starting material used. The reactivity of the starting material also can be conducted under nucleophilic substitution-type conditions or electrophilic substitution-type conditions. For example, nucleophilic substitution-type conditions involve using a nucleophile, such as a sulfur-containing nucleophile (e.g., —S-(hetero)alkyl, —S-(hetero)alkenyl, —S-(hetero)alkynyl, —S-(hetero)aryl, —S-(hetero)alkyl(hetero)aryl, —S-(hetero)alkenyl(hetero)aryl, —S-(hetero)alkynyl(hetero)aryl), an amine-containing nucleophile (e.g., —NR3-(hetero)alkyl, —NR3-(hetero)alkenyl, —NR3-(hetero)alkynyl, —NR3-(hetero)aryl, —NR3-(hetero)alkyl(hetero)aryl, —NR3-(hetero)alkenyl(hetero)aryl, —NR3-(hetero)alkynyl(hetero)aryl), a hydroxyl-containing nucleophile (e.g., —O-(hetero)alkyl, —O-(hetero)alkenyl, —O-(hetero)alkynyl, —O-(hetero)aryl, —O-(hetero)alkyl(hetero)aryl, —O-(hetero)alkenyl(hetero)aryl, —O-(hetero)alkynyl(hetero)aryl), or a carbon-based nucleophile (e.g., alkyl-MgBr, alkylaryl-MgBr, alkenyl-MgBr, or alkynyl-MgBr) to displace a reactive group of the starting material. In electrophilic substitution-type conditions, the starting material reacts with a suitable electrophile to replace the reactive groups on the starting material.
In particular disclosed embodiments, the starting material satisfies Formula 9, illustrated below.
With reference to Formula 9, each of X1, X2, and X3 is a reactive group that can be substituted using nucleophilic substitution-type conditions or electrophilic substitution-type conditions. For example, under nucleophilic substitution-type conditions, each reactive group X1, X2, and X3 independently is a leaving group selected from a halogen, a perfluoroalkylsulfonate, a sulfonate, or a carboxylate. In some embodiments, each of X1, X2, and X3 independently can be selected from bromo, fluoro, chloro, iodo, triflate, tosylate, mesylate, besylate, or —OCOalkyl (e.g., —OCOMe, —OCOEt, —OCOiPr). These reactive groups are then sequentially displaced with a nucleophile selected from a thiol, an amine, an alcohol, or a carbon-based nucleophile (e.g., a Grignard reagent). In some embodiments, an increase in temperature or addition of base can be used to facilitate nucleophilic displacement of the reactive groups. Under electrophilic substitution-type conditions, an electrophile is reacted with the starting material and a Lewis acid to facilitate addition of the electrophile to the core of the starting material. Removal of a reactive group, typically a hydrogen atom, is then facilitated with a base, thereby restoring aromaticity to the starting material core. Exemplary electrophiles include alkene-containing or alkyne-containing compounds, sulfuric acid, or an acyl-containing compound.
In some embodiments, starting materials can be used wherein the core of the starting material has two reactive groups, with the third available carbon atom of the triazine ring being substituted with a hydrogen atom. In such embodiments, these starting materials can serve as molecular precursors or submonomers for forming part of a heteropolymer or homopolymer, or a terminal group. As part of forming a heteropolymer or homopolymer, the hydrogen atom can constitute a side chain of a monomer of the heteropolymer or homopolymer.
In exemplary embodiments, the starting material can be cyanuric chloride (or 2,4,6-trichloro-1,3,5-triazine), or 2,4-dichloro-1,3,5-triazine, which has the structures illustrated below, respectively.
In some embodiments, reactions on the starting material that add side chains, linker segments, terminal groups, or combinations thereof, can be used to create molecular precursors and/or submonomers. Molecular precursors typically comprise at least one reactive group that has not been substituted (or replaced). In some embodiments, molecular precursors can comprise additional atoms or functional groups that are lost in bond forming reactions to thereby form monomer units when the polymer chain is assembled. Molecular precursors and submonomers can be used to form repeat monomer units within a polymer chain. A repeat monomer unit is referred to herein as a monomer unit (or combination of monomer units) of a polymer that repeats within the polymer chain. In some embodiments, a repeat monomer unit can refer to the core of a monomer unit of a polymer chain (for example, a core ring A as illustrated in Formula 1, or, with reference to Formulas 2-6 or Formula 8, a triazine ring). In such embodiments, the repeat monomer unit may have the same core, but can vary from other repeat monomer units in the polymer chain by variations in the side chain, terminal group, and/or linker groups of the repeat monomer unit structure. In other embodiments, a repeat monomer unit can be an entire monomer unit within a polymer chain, such as would be the case with a homopolymer, such as that illustrated in Formula 7. In some embodiments, a submonomer is used to form the terminal group on a polymer described herein.
In exemplary embodiments, submonomers can have structures that satisfy Formula 10.
With respect to Formula 10, the starting material is monosubstituted with a side chain group, indicated by R—Y, thereby forming a submonomer having a side chain that will be pendant to a polymer formed with the submonomer. In some embodiments the side chain may be a single atom or group of atoms. In some embodiments, the side chain structure can be an atom or group of atoms derived from any molecule or reagent that can displace (or replace) a reactive group from the starting material, and so become covalently attached. In exemplary embodiments, each of X2, X3, Y1, and R1 can be as recited herein. In some embodiments, Y1 can be a bond and R1 can be hydrogen. In an independent embodiment, if Y1 is NH, then R1 is other than, or is not H. In some other embodiments, if Y1 is NR4, wherein R4 is a butyl group, then R1 is other than, or is not, a butyl group.
In some embodiments, heteroatoms in the side chain may be coupled (e.g., covalently or electrostatically coupled) to a protecting group that renders the heteroatoms unreactive under the conditions of polymer synthesis. Suitable protecting groups can be selected from protecting groups known in the art, such as those disclosed by Greene & Wuts, Protective Groups in Organic Synthesis, 4th Edition, John Wiley & Sons, Inc., New York (2007), the relevant portion of which (e.g., sections discussing nitrogen, oxygen, sulfur, and phosphorus protecting groups) is incorporated herein by reference. In embodiments of submonomers or molecular precursors wherein nucleophilic displacement is used to couple a side chain to the starting material, the side chains are attached by atoms of the Y from nucleophilic molecules, such as but not limited to N, S, or O present in amines, thiols, or alcohols, respectively. In some embodiments, the side chain is attached by a Y group wherein Y is coupled to the starting material through a carbon atom using nucleophilic conditions and carbon nucleophiles, such as but not limited Grignard reagents or alkyl lithium reagents. In some embodiments, the side chain is a bond and a hydrogen atom. In some embodiments, a molecular precursor or submonomer comprising a single side chain can be used as a precursor in polymer synthesis. Exemplary submonomers comprising side chains are illustrated below in Table 2.
In some embodiments, submonomers can have structures satisfying Formula 11, illustrated below.
With reference to Formula 11, the starting material is monosubstituted with a linker group (e.g., W1-L1-Z1) thereby forming another embodiment of a submonomer. In some embodiments, each of X1, X3, W1, L1 are as recited herein. Z1 can be as indicated previously, but when Z1 is not part of a polymer chain as illustrated in Formula 10 (or any of the subsequent formulas), Z1 is terminated with an atom or functional group that can be removed upon reaction with another molecular precursor or submonomer to thereby form a covalent linkage. For example, Z1 can be terminated with one or more hydrogen atoms or protecting groups. Exemplary submonomers comprising linkers are illustrated below in Table 3.
A linker group comprises a sequence of atoms that will join the cores in two consecutive monomers in the polymer. As illustrated in Formula 1, the linker sections can be used to join two rings, such as the triazine rings of Formulas 2-8, and become part of the overall backbone of the final polymer. In the molecular precursors or submonomers disclosed herein, the linker group can be a W1-L1-Z1 group, wherein L1 is coupled to an additional atom or functional group, namely W1, which links L1 to the molecular precursor or the submonomer. L1 also can be coupled to Z1, which functions as a nucleophile (or electrophile) to attach one molecular precursor or submonomer to another molecular precursor or submonomer. In some embodiments, the two ends of each linker section (e.g., W1 and Z1) can be the same or different. Exemplary linker groups are illustrated in Table 4.
In yet additional embodiments, submonomers can have structures satisfying Formula 12. Such submonomers comprise a terminal group, T, which can be as defined above.
Molecular precursors disclosed herein can comprise a combination of a side chain and linker moieties, and can have structures satisfying Formula 13. In some polymer embodiments, these molecular precursors will become monomer units in the polymer chain, as described above.
With reference to Formula 13, each of R1, Y1, X3, W1, L1, and Z1 can be as recited herein. Examples of molecular precursors and molecular monomers are shown in Table 5.
In yet additional embodiments, molecular precursors can have combinations of side chains and terminal groups, or terminal groups and linkers, as illustrated below in Formulas 14 and 15, respectively.
In additional embodiments, the molecular precursors can be further functionalized to form molecular monomers having structures satisfying Formulas 16, 17, or 18.
With reference to Formula 16, a reactive group (e.g., X3) is substituted (or functionalized) with V1, which can be an atom or functional group that becomes covalently linked to another molecular precursor or submonomer to form a polymer backbone. In particular disclosed embodiments, V1 can be a J1-L4-G1 group, as illustrated in Formula 17. The reaction of J1 with a Z1 group of another molecular precursor or submonomer can result in forming a covalent bond to form a polymer backbone. With reference to Formula 17, G1 can be a heteroatom selected from O, N, S, or P, or G1 can be a single bond to L4; L4 can be selected from any one of the atoms or groups disclosed for L1, L2, or Lq; and J1 can be terminal alkenyl or alkynyl group, or a thiol or azide capable of reacting with Z. In exemplary embodiments, J1 can be a double bond and Z1 could be a thiol, or J1 could be a triple bond and Z1 could be an azide. Alternatively, Z1 can be a double bond and J1 could be a thiol, or Z1 could be a triple bond and J1 could be an azide.
With reference to any one of Formulas 12, 14, 15, or 18, the terminal group can be present on the submonomer, molecular precursor, or molecular monomer before the assembly of the polymer. Terminal groups present at the beginning of polymer synthesis typically are unreactive under the polymer forming reaction conditions disclosed herein. In some embodiments, a terminal group can be a group provided upon completion of polymer synthesis. In some embodiments, a terminal group can be provided upon completion of polymer synthesis by cleaving an assembled polymer from a solid support. After the polymer is cleaved from the solid support, the terminal group may be the atom or functional group remaining on the terminus of the polymer after cleavage. Exemplary structures with terminal groups are provided in Table 6.
Disclosed herein are methods of making unique polymers that can be used for a variety of applications, such as materials science applications, bioenergy applications, nanotechnology, separation science, membranes, molecular recognition, assays, chemical or biomolecular sensors, biomedical therapy, pharmaceuticals, catalysis, and/or information storage. These method embodiments can comprise making sequence-defined polymers wherein the polymer chain comprises three or more structurally different monomer units in a predetermined sequence. In such embodiments, the polymer is of a defined length resulting from the predetermined number of monomer units in the sequence and can comprise terminal groups on each end. In some embodiments, the methods disclosed herein can be used to make any of the polymers described herein (e.g., sequence-defined polymers, block copolymers, sequence-controlled polymers, and/or homopolymers). In particular disclosed embodiments, the methods disclosed herein are used to make heteropolymers or homopolymers that have a defined length and therefore are monodisperse, or substantially monodisperse.
The methods disclosed herein are distinguished from conventional batch polymerizations that can produce repeating patterns but that do not produce polymers having a defined length, and are therefore polydisperse polymers. Conventionally, making monomer components that can be assembled into sequence-defined polymers having multiple different repeat monomer units in the sequence is very challenging and conventional methods do not provide the same control over polymer length, identity, and/or purity as the methods disclosed herein.
It has been discovered that the novel polymers disclosed herein can be made of a particular length and/or to have a pre-determined sequence using the molecular precursors, submonomers, and/or molecular monomers disclosed herein. In some embodiments, the molecular precursors, submonomers, and/or molecular monomers comprise at least two points of attachment that can be used to form bonds to produce the polymer backbone. Also, the molecular precursors, submonomers, and/or molecular monomers comprise additional features that allow variation of the monomer units or repeat monomer units of the polymer. In some embodiments, an additional feature can be a reactive group that can be displaced or replaced with a side chain that can be used to distinguish one monomer unit from another and also contribute to the ultimate unique functional properties of the polymers. Accordingly, it has been discovered that starting materials comprising multiple reactive sites (e.g., three reactive sites) can be used. Given the level of difficulty in controlling the degree of substitution to obtain different substituents on each of the multiple reactive sites, traditional methods of making monomers with three different reactive sites make complex mixtures that are difficult to separate and thereby reduces overall yield of the components used to make polymers.
The methods of making polymers using the submonomers, molecular precursors, and/or molecular monomers disclosed herein, however, surprisingly solve the problems that have long plagued traditional methods for making tri-functional compounds and polymers of such compounds. In particular disclosed embodiments, combinations of temperature and reagent control can be used to make the submonomers, molecular precursors, and/or molecular monomers and also to make polymers comprising such components. The polymer components disclosed herein can be made using starting materials that contain three reaction sites, which are sequentially reactive with excellent control. These starting materials are selected to have the reactivity discussed herein, with particular examples being found available commercially (or are otherwise readily available from commercially available starting materials) and also are inexpensive in comparison with alternative molecular precursors traditionally used in the art. Additionally, this chemistry can be used to assemble polymers by submonomer methods, in addition to chain extension with protected molecular precursors followed by deprotection.
The starting materials, submonomers and molecular precursors used in polymer synthesis can comprise at least one reactive groups that is less reactive than at least one other reactive group. For example, the starting materials disclosed herein comprise three reactive groups, each of which becomes less reactive as each substitution (or replacement) of such groups occurs. Thus, the sequential reactivity of the reactive groups, and therefore polymer formation, can be controlled by modifying reaction conditions, such as by increasing the temperature of the reaction and/or using a more reactive reagent (or reagents) to convert the reactive groups to a different chemical moiety. In some embodiments, a higher reaction temperature can be a temperature that is higher than that used to convert a previous reactive group. In yet other embodiments, reactive conversion materials can be used to convert a subsequent reactive group to a different functional group, as compared with that used for a previous reactive group. These methods embodiments represent a unique way to obtain a vast library of submonomers, molecular precursors, and/or molecular monomers having the functionalities needed to make the polymers disclosed herein and thereby enable multiple approaches to assembly of polymers having useful functionality. The versatility of these novel methods in creating unique functional polymers of myriad varieties is illustrated in the methods of making polymers disclosed below.
In some embodiments, the methods disclosed herein can comprise coupling, at a first temperature, a first position of a first starting material with a terminal group; coupling, at a second temperature, a second position of the first starting material with a linker group or a side chain group; coupling, at a third temperature, a third position of the first starting material with a linker group or a side chain group to form a first monomer unit of the polymer; coupling, at the first temperature, the first monomer unit of the polymer with a first position of a second starting material; coupling, at the second temperature, a second position of the second starting material with a linker group or a side chain group; coupling, at the third temperature, a third position of the second starting material with a linker group or a side chain group to form a second monomer unit of the polymer; coupling, at the first temperature, the second monomer unit of the polymer with a first position of a third starting material; coupling, at the second temperature, a second position of the third starting material with a linker group or a side chain group; and coupling, at the third temperature, a third position of the third starting material with a linker group or a side chain group to form a third monomer unit of the polymer; wherein the polymer has a formula according to any one of Formulas 2-8. In some embodiments, the linker group comprises a protecting group and the method further comprises exposing the linker group to an acid or a base to remove the protecting group. In some embodiments, the terminal group comprises a solid support and the method further comprises exposing the polymer to an acid to remove the solid support.
In other embodiments, the methods disclosed herein can comprise making a heteropolymer. Such embodiments can comprise coupling a first position of a first molecular precursor comprising a first side chain, a first linker group, or both with a terminal group; coupling a second position of the first molecular precursor with a second molecular precursor comprising a second side chain, a second linker group, or both thereby forming a covalent bond between the first molecular precursor and the second molecular precursor; and coupling the second molecular precursor with a third molecular precursor comprising a third side chain, a third linker group, or both thereby forming a covalent bond between the second molecular precursor and the third molecular precursor; wherein the first side chain is different from one or both of the second side chain or the third side chain, and wherein the heteropolymer has a formula according to any one of Formulas 2-8. In some embodiments, each of the first linker group, the second linker group, and the third linker group comprises a protecting group and the method further comprises exposing the protecting group to an acid or a base to facilitate coupling the first molecular precursor, the second molecular precursor, and the third molecular precursor. In some embodiments, the terminal group comprises a solid support, and the method further comprises exposing the solid support to an acid to cleave the solid support from the terminal group.
In yet other embodiments, the methods can comprise selecting a first submonomer, a second submonomer, and a third submonomer, wherein at least two of the first, second or third submonomers are different; coupling the first submonomer, the second submonomer, and the third submonomer with one of a terminal group, a linker group, or a side chain; and coupling the first submonomer, the second submonomer, and the third monomer to form a polymer having a formula according to any one of Formulas 2-8. In some embodiments, the linker group comprises a protecting group and the method further comprises exposing the protecting group to an acid or base to remove the protecting group. In some embodiments, the terminal group comprises a solid support and the method further comprises exposing the solid support to an acid to cleave the solid support from the terminal group. In some embodiments, the method further comprises coupling the first submonomer, the second submonomer, and the third submonomer in a nonrandom sequence that is selected before making the polymer. The first submonomer can be coupled to the terminal group and the linker group. In some embodiments, the first submonomer is coupled to the second submonomer through the linker group. The second submonomer can be coupled to another linker group and the linker group is subsequently coupled to the third submonomer. In any of the disclosed embodiments, the method can further comprise terminating polymerization, such as by cleaving a polymer from a solid support or exposing the polymer chain to a terminal group that prevents further polymerization.
Scheme 1 illustrates exemplary methods that can be used to produce submonomers, molecular precursors, molecular monomers, and polymers disclosed herein.
With reference to Scheme 1, a first reactant nucleophile (e.g., R1—Y1(H)) can displace a reactive group (e.g., X1) of the starting material at ice temperature, such as −25° C. to 5° C., and the reaction will stop at monosubstitution, yielding “RXX.” Excess nucleophile can be separated from the product before reaction with a second nucleophile at a higher temperature to form “RAX,” and so on to form “RAB.” In such embodiments, a tri-functional molecule can be constructed around a core with excellent control leading to three different substituents, and such molecules may be used for forming polymer structures.
Exemplary embodiments of the methods summarized in Scheme 1 are illustrated in Scheme 2, below.
Schemes 1 and 2 illustrate the surprising versatility of this approach for creating submonomers, molecular precursors, and molecular monomers efficiently and with excellent control of substitution to enable creation of polymers. These particular methods can be modified as illustrated below to produce the various different types of polymers disclosed herein. Some embodiments concern making polymers by adding submonomers or molecular precursors to the growing polymer chain one at a time, or assembling monomer units from starting materials that have been incorporated into the growing polymer chain one at a time. These exemplary synthetic schemes demonstrate different method embodiments contemplated herein.
Schemes 3 and 4 illustrate generalized approaches for sequence-defined polymers. Scheme 3 describes polymer chain extension using a protected molecular precursor followed by deprotection. Scheme 4 describes a submonomer method embodiment without protecting groups wherein two submonomers (e.g., a functionalized starting material and a linker) are combined to form monomer units of a growing polymer chain. As illustrated in Scheme 4, polymerization is initiated by reacting a first submonomer with a terminal group at one temperature and then reacting the combined submonomer with another submonomer (e.g., a linker group) at a higher temperature, thus extending the polymer by one full monomer unit.
An exemplary embodiment of the method illustrated in Scheme 3 is provided below in Schemes 5A and 5B. Scheme 5A illustrates polymer chain extension by a selected molecular precursor with a side chain Rn—Yn whose linker section containing Ln is protected, followed by deprotection to enable the next step in chain extension. Scheme 5B illustrates a particular disclosed embodiment wherein Y is sulfur and the linker is ethylene diamine.
Exemplary embodiments of the submonomer method illustrated in Scheme 4 are illustrated in Schemes 6A-6D. Schemes 6A and 6B illustrate exemplary submonomer methods of making a polymer wherein no protecting groups are used. A submonomer comprising a side chain is added to extend the polymer chain. In these and other schemes provided herein, the extension of the polymer chain can be stopped either after a linker group W-L-Z has been added to the end, or after the previous step where a submonomer has been added. In embodiments wherein extension of the polymer chain is stopped after a previous step wherein a submonomer has been added, additional reactions can be performed to displace the remaining chlorine atom on the end of the polymer, creating a terminal group Tf. In some embodiments, the reaction can yield a Tf group equivalent to —NH2. Schemes 6C and 6D illustrate exemplary submonomer methods of making a polymer wherein protecting groups can be used. In Scheme 6C, a linker submonomer comprising a protecting group is added to the growing polymer chain by reacting the protected linker submonomer with a submonomer of the growing polymer chain. In Scheme 6D, a molecular precursor comprising a protected linker group is added to the growing polymer chain and subsequently deprotected to facilitate reaction with another molecular precursor.
In some embodiments, the methods disclosed herein can comprise using a starting material at the beginning of each polymerization cycle rather than a molecular precursor or a submonomer. Exemplary embodiments are illustrated below in Schemes 7A-7C. As illustrated in Scheme 7B and 7C, these method embodiments can further comprise protection and deprotection steps.
An exemplary embodiment of a method using sequential deprotection/addition steps for polymerization, related to the more generalized embodiments shown in certain schemes provided above is illustrated in Scheme 8.
In some embodiments, any of the polymerization methods illustrated above can be used in combination with a solid support to facilitate making polymers. The solid support can be included as part of the terminal group. In some embodiments, these methods, as well as any of the methods described herein, can be automated. An exemplary embodiment of using solid support synthesis is illustrated below in Schemes 9A and 9B.
In yet other embodiments, solid support synthesis of polymers can be performed with modified reaction conditions that allow for polymer synthesis without deprotection/addition step sequences and/or the use of a library of fully formed monomers. For example, as illustrated below in Schemes 10A and 10B, polymers can be made on solid supports and substitution and temperature parameters can be used to regulate the molecular precursor or submonomer reactivity. The solid support can be used to prevent at least one end of a linker group from undesirably reacting with multiple monomer precursors and/or submonomers and only two reaction steps in sequence are needed to create the polymer chain. Also, no acid reagents are required to make the polymer in this embodiment, therefore the solid support (which often is cleaved under acidic or basic conditions) can be conserved during chain extension. The solid support can then be cleaved using acidic or basic conditions once the desired chain length has been obtained.
Exemplary embodiments of a polymerization method using a terminal group comprising a solid support is illustrated below in Scheme 11. This method embodiment provides the ability to make polymers having a specified length with a variety of monomer combinations.
Additional exemplary solid-phase synthetic methods are illustrated below in Schemes 12-14.
Additional independent exemplary synthetic methods are illustrated in Schemes 15-19.
In an independent embodiment, the method illustrated in Scheme 20, below, can be used to make a singly branched polymer embodiment. While Scheme 20 illustrates a particular embodiment of a monomer used to make the polymer, one or more of the monomer embodiments disclosed herein can be used in the method. Other methods of making such singly branched polymer embodiments can include using the method steps discussed above for the linear polymers to produce a first arm, and further comprising reacting a starting material with the growing polymer chain (or first arm) rather than another submonomer or molecular precursor, thereby creating a branch point by functionalizing one of the reactive groups on the starting material with a group of the growing polymer chain. The remaining reactive groups can then be displaced with linker groups and polymerization can be carried out on both linker groups using the methods described above.
In particular disclosed embodiments, the monodispersity of the polymers arises from the specific number of monomer units created in the course of starting and extending the polymer chain. In particular disclosed embodiments, the degree of polymerization and/or monodispersity obtained from using the above-disclosed methods can be determined based on the weight of the individual monomer components. For example, in embodiments where a homopolymer is made, the molecular weight of each individual monomer component can be determined and the degree of polymerization can be determined (or the length of the polymer can be confirmed) by dividing the number-average molecular weight or the molecular weight of the polymer by the molecular weight of the monomer component. In yet other embodiments, the length of the polymer can be confirmed using an analytical technique, such as mass spectrometry, chromatography (e.g., HPLC or gel-permeation chromatography), nuclear magnetic resonance (NMR) spectrometry, Infra-red spectroscopy, dynamic light scattering, or the like.
In some embodiments, the polymers disclosed herein can comprise 3 to 1000 monomer units, such as 3 to 500 monomer units, 3 to 100 monomer units, 3 to 50 monomer units, 3 to 10 monomer units, or 3 to 6 monomer units. In some embodiments, the polymers can comprise at least 3 monomer units up to 1000 monomers, at least 4 monomer units up to 1000 monomers, at least 5 monomer units up to 1000 monomers, or at least 6 monomer units up to 1000 monomer units. In other embodiments, the polymer can comprise at least 3 monomer units up to 500 monomers, at least 4 monomer units up to 500 monomers, at least 5 monomer units up to 500 monomers, or at least 6 monomer units up to 500 monomer units. In yet other embodiments, the polymer can comprise at least 3 monomer units up to 100 monomers, at least 4 monomer units up to 100 monomers, at least 5 monomer units up to 100 monomers, or at least 6 monomer units up to 100 monomer units.
In some embodiments, the polymers can comprise 6 to 100 monomer units, 6 to 50 monomer units, 6 to 20 monomers, or 6 to 12 monomers. In some embodiments, the polymers disclosed herein can comprise 4 to 1000 monomer units, such as 4 to 500 monomer units, 4 to 100 monomer units, 4 to 50 monomer units, 4 to 10 monomer units, or 4 to 6 monomer units. In some embodiments, the polymers disclosed herein can comprise 5 to 1000 monomer units, such as 5 to 500 monomer units, 5 to 100 monomer units, 5 to 50 monomer units, 5 to 10 monomer units, or 5 to 7 monomer units.
In the disclosed heteropolymers, at least one of the monomer units differs from the other monomer units of the polymer. For example, each of the 3 to 1000 monomer units can be different, with some embodiments having the number of different monomer units ranging from 2 to 1000, such as 2 to 500 monomer units that are different, 2 to 100 monomer units that are different, 2 to 50 monomer units that are different, 2 to 10 monomer units that are different, or 2 to 6 monomer units that are different. For example, each of the 3 to 1000 monomer units can be different, with some embodiments having the number of different monomer units ranging from 3 to 1000, such as 3 to 500 monomer units that are different, 3 to 100 monomer units that are different, 3 to 50 monomer units that are different, 3 to 10 monomer units that are different, or 3 to 6 monomer units that are different. For example, each of the 3 to 1000 monomer units can be different, with some embodiments having the number of different monomer units ranging from 4 to 1000, such as 4 to 500 monomer units that are different, 4 to 100 monomer units that are different, 4 to 50 monomer units that are different, 4 to 10 monomer units that are different, or 4 to 6 monomer units that are different. For example, each of the 3 to 1000 monomer units can be different, with some embodiments having the number of different monomer units ranging from 5 to 1000, such as 5 to 500 monomer units that are different, 5 to 100 monomer units that are different, 5 to 50 monomer units that are different, 5 to 10 monomer units that are different, or 5 to 6 monomer units that are different. In some embodiments, the polymers can comprise 6 to 100 monomer units that are different, 6 to 50 monomer units that are different, 6 to 20 monomer units that are different, or 6 to 12 monomer units that are different. In the disclosed homopolymers, each of the monomer units is the same.
In some embodiments, the materials made from the disclosed polymers can be characterized using techniques recognized by those of ordinary skill in the art to be suitable for characterizing, for example, nano-sized materials. Such methods can include atomic force microscopy (AFM), transition electron microscopy (TEM), and in situ TEM methods. In yet some other embodiments, single crystal x-ray crystallography can be used to determine structure and polymer conformation within diffracting ordered crystals formed with the polymers.
Described below are methods for making the molecular precursors, submonomers, and molecular monomers disclosed herein. In particular disclosed embodiments, a starting material having multiple reactive groups can be reacted with suitable reagents to form a submonomer or molecular precursor. In some embodiments, a first reactive group of the starting material can be reacted under conditions suitable to convert only one of the reactive groups to a side chain, linker group, or terminal group, such as by reacting a triazine moiety with a nucleophile under basic conditions, as illustrated in Scheme 21, or by reacting a triazine moiety with an electrophile using a Lewis acid. Suitable bases that can be used include, but are not limited to organic bases (e.g., diisopropylethylamine, lithium diisopropylamine, or other amine bases know to those of ordinary skill in the art) or inorganic bases (e.g., potassium carbonate, sodium hydroxide, lithium hydroxide). Solvents that can be used for these types of reactions include, but are not limited tetrahydrofuran or dichloromethane. Suitable nucleophiles and electrophiles are disclosed herein.
Exemplary methods of making molecular precursors are illustrated below in Schemes 22-25. In some embodiments, such as the exemplary embodiment illustrated by Scheme 25, a reactive group can be converted to a linker group or a terminal group prior to adding the side chain. For example, as illustrated in Scheme 25, a linker group is first reacted with the starting material to form a submonomer and then the submonomer is functionalized with a side chain to produce a molecular precursor. Scheme 25 further illustrates an embodiment wherein the linker group comprises a protecting group, and wherein the linker group can be added to the molecular precursor to provide a protected monomer that can then undergo deprotection and subsequent polymerization.
The submonomers, molecular precursors, and/or molecular monomers disclosed herein can be used to make polymers that mimic the ability of naturally-occurring sequence-defined polymers to form conformers, biomaterials, encode information, perform biocatalysis, participate in molecular recognition, and shuttle species across membranes. The polymers disclosed herein also can be used to mimic the functionality of poly(peptides), which are constructed of sequences of amino acids, each amino acid having its own side chain group that is pendant to the alpha-carbon, while the backbone consists of a regular pattern of amino-N, alpha-C, and carbonyl-C functional groups. While the polymers may mimic such functionality and/or behavior, they have structural features that are separate and distinct from naturally-occurring and/or synthetic polypeptides, which contribute to their superior stability and functionality.
In some embodiments, the polymers disclosed herein can be used as biomimetic polymers, which are useful for materials science and bioenergy applications, such as CO2 separation. In particular disclosed embodiments, the polymers exhibit high thermostability and resistance to biodegradation. The polymers disclosed herein also can form artificial beta sheets, artificial alpha helices, and the like that can be used to target biopolymers, disrupt bacterial membranes, and/or permeate cells, based on interactions between the monomers making up the polymers. The polymers also can self-assemble into particular materials, such as membranes, nanosheets, crystals, nanocapsules, microcapsules, nanotubules, microtubules, liquid crystals, fibers, nanofibers, or composite materials that contain additional components such as ions, minerals, or molecules and macromolecules
Composite materials containing a polymer and nanostructures can be used in organic solar cells, catalysis, chem/bio sensing, and flexible electronics. Achieving a homogeneous blend of the two components or a precise spatial distribution of one within the other, e.g. along air-solid interfaces in porous materials, is a goal often sought in the art. Poor spatial control within polymer/nanostructure composites, however, is a deficiency that has not yet been efficiently addressed by traditional methods. Traditionally, 3D composite materials of polymers and nanometer-sized components are synthesized using empirically determined parameters to optimize a product's properties, without elucidating or developing predictive methodologies that address the mechanism of nanoparticle assembly or distribution in these compounds and subsequently their properties. Such traditional methods typically rely on blending, electrostatically-binding, or tethering nanostructures to existing polymer chains. In contrast, the presently disclosed polymers and methods of making such polymers can be used to determine a reaction mixture's intrinsic and polymerization-induced self-assembly mechanisms, thus providing the ability to determine the underlying principles that govern the self-assembly of functionalized nanostructures under different conditions, such as overall monomer concentration, temperature, etc. In some embodiments, a parameter map based on these observations can be developed, which can be used to design functional materials in which nanometer-sized components can be placed within a polymer matrix with spatial precision.
The sequence-defined polymers are novel, structurally distinct mimetics to biopolymers with defined sequences, such as poly(nucleic acids) and polypeptides. The sequence-defined polymers disclosed herein can comprise side chains on monomer units, wherein these monomer units are arranged in predetermined sequences within the polymer chain, and having defined lengths. Structures and methods for making sequence-defined polymers are difficult to originate, as compared to random copolymers. The very specifically defined side chains, monomer unit sequences, and lengths of sequence-defined polymers are particularly useful, however, with a wide variety of uses by nature and by human application, including but not limited to functions to serve as materials or self-assembling materials, encode information, perform catalysis, participate in molecular recognition, regulate biological processes, perform signaling, and shuttle species across membranes.
Naturally occurring sequence-defined polymers are known to self-organize into particular conformations, such as the alpha helices and beta sheets and helical bundles of polypeptides, and the double stranded helix of DNA. The disclosed polymers can assemble with themselves (self-assembly) or with other complementary molecules or macromolecules (molecular recognition). Molecules and materials with these properties have therapeutic or other pharmacologic biomedical effects and can be useful in assays, in sensors or separations, they also can target bacteria, kill pathogens, or perform catalysis. Polymers that self-assemble through intermolecular interactions, such as the polymers disclosed herein, can form membranes, nanosheets, crystals, nanocapsules, microcapsules, nanotubules, microtubules, liquid crystals, fibers, nanofibers, or composite materials that contain additional components such as ions, minerals, or molecules and macromolecules. In some embodiments, the polymers can form composite materials with inorganic nanoparticles or nanostructures, such as ZnO or Au, wherein the polymers can impart an element of secondary structure to the nanoparticle distribution within a polymer matrix.
The great structural diversity of the molecules and materials disclosed herein, and methods of making them, will thus enable many useful applications. Exemplary embodiments are described below.
As shown in
In some embodiments, interactions between functional groups of the monomer units of the polymers are available to influence intramolecular conformational structure and/or intermolecular self-assembly, such as backbone/backbone hydrogen bonding or side-chain/side-chain interactions. Molecular simulations can be used to determine such interactions, as well as to determine structural conformations of the polymers that can be formed in solution. In some embodiments, the disclosed polymers can participate in hydrogen-bonding based interactions, create helical foldamers, and/or assemble into ribbon structures. For example,
Structural modeling of different sequences of the polymers disclosed herein, in which (in some embodiments) variable-length non-amide linkers are used to connect monomers, can be used to inform experimental designs. The monomer units and linkers of the polymers disclosed herein can be parameterized using a generalized amber force field, and 500-ns REMD simulations can be used to explore the conformational space of the polymers. K-means clustering also can be used to identify potential structures of interest. In particular disclosed embodiments, simulations of hexamers suggest several favored motifs with different backbone/backbone hydrogen bonding and π stacking configurations. The simulations also suggest that, in some embodiments, imine-containing functionalized groups can increase helical propensity, that chiral linkers can increase conformational order, and that longer linkers can promote more globular structures. In particular embodiments concerning simulations of 12-mers, more globular and elaborate tertiary structures can be formed, which are reminiscent of proteins. Such simulations confirm the applicability of the polymer embodiments disclosed herein in applications where intramolecular conformational and structural order are functionally important.
In some embodiment, molecular dynamics simulations on polymer structures can be performed to determine the favored conformations of a given polymer structure in solution. These particular structures can be visualized and examined to observe the overall conformations and the interactions, such as backbone-backbone hydrogen-bonding and pi-pi interactions, which contribute to holding the polymer chains in such conformations. In addition, structures can be generated varying such features as the linker segment to see the influence on the conformational behavior.
Polymer structures can be constructed in extended starting conformations using an in-house python script. Simulations can first be carried out in implicit solvent representing a high dielectric constant environment similar to water, using a replica exchange molecular dynamics method to accelerate the process and obtain conformational results. The particular desired conformations, or conformations of interest, can be determined, such as those that are most likely to represent stable conformations rather than unfolded or transient structures. Simulations also can be carried out in explicit water solvent, typically starting with a conformer identified in the implicit solvent simulation to determine if the conformer is stable in explicit solvent environment.
Molecular simulations on polymer sequences disclosed herein have revealed detailed motifs of interactions that contribute to conformational or self-assembly behaviors establishing the utility of the polymers disclosed herein in a variety of applications. In some embodiments, molecular simulations of the polymer structures support backbone-backbone interactions by hydrogen bonding and by pi-pi interactions.
From the simulations results illustrated in
Some exemplary structural fragments representing hydrogen bonding motifs found in particular molecular dynamics simulations are provided below in Table 7. The first example in Table 7 illustrates the hydrogen bonding motifs that bind the 2nd and 5th triazine rings from the conformer illustrated in
Additional entries in Table 7 show where a hydrogen bond occurs “along the chain” and stabilizes a turn in the direction of the polymer chain (entry four), and an example where such a turn is stabilized by additional backbone-backbone hydrogen bonds (entry 5). These hydrogen bonding motifs illustrate the rich interactions that the disclosed polymer chains can participate in, and these will contribute to conformational structures and self-assembly mechanisms. The paired hydrogen bonds in exemplary polymers disclosed herein are compared with paired hydrogen bonds in peptide beta sheets and DNA base pair interactions in Entry 7.
Additional simulations of other exemplary polymer structures have been carried out with a variety of side chains and linkers. The components of these additional simulated polymer structures are provided in Table 8.
Selected examples of conformations found in the molecular dynamics simulations are illustrated in
Additional structures can also be made using the molecular precursors, submonomers, and methods of combining such components disclosed herein. Some exemplary interactions are illustrated below.
In some embodiments, branched polymers can be produced, such as that illustrated in Scheme 20. Given hydrogen bonding motifs already described, with multiple backbone-backbone hydrogen bonds, the arms between one branched polymer and another branched polymer may interact as illustrated below (wherein the branched side arms and the core triazine ring are illustrated).
An exemplary embodiment wherein this type of interaction produces networks of branched macromolecules is illustrated in
Whereas
Reagents and General Procedures.
Reagents were obtained from commercial sources and used as purchase. Organic solvents were purchased anhydrous and used without further purification. Unless otherwise noted, all reactions were carried out at room temperature in oven-dried glassware with magnetic stirring. Organic solutions were concentrated under diminished pressure with bath temperatures <40° C. Flash column chromatography was carried out on silica gel G60 (Silicycle, 60-200 μm, 60 Å). Thin-layer chromatography (TLC) was carried out on Silica gel 60 F254 (EMD Chemicals Inc.) with detection by UV absorption (254 nm) were applicable, and by spraying with 20% sulfuric acid in ethanol followed by charring at ˜150° C. or by spraying with a solution of (NH4)6Mo7O24.H2O (25 g/L) in 10% sulfuric acid in ethanol followed by charring at ˜150 OC. 1H and 13C NMR spectra were recorded on a Varian Inova-500 (500/125 MHz) equipped with sun workstations. Multiplicities are quoted as singlet (s), broad singlet (br s), doublet (d), doublet of doublets (dd), triplet (t), or multiplet (m). Spectra were assigned using COSY, DEPT and HSQC experiments. All chemical shifts are quoted on the δ-scale in parts per million (ppm). Residual solvent signals were used as an internal reference. Reverse-Phase HPLC was performed on an Aglient 1100 series system equipped with an auto-sampler, fraction-collector, UV-detector and Aquasil-C18 column (5 μm particle size, 4.6×250 mm, 100 Å pore size) at a flow rate of 1.0 mL/min. Mass spectra were recorded on an Agilent Technologies 6530 Accurate-Mass Q-TOF LC/MS. The calibrant is Standard ESI-L low concentration Tuning Mix with addition of 0.1 mM HP-0321.
To a stirred and cooled (0° C.) solution of cyanuric chloride (2.60 g, 14.1 mmol) and N,N-diisopropylethylamine (2.70 mL, 15.5 mmol) in THF (40 mL) was added thiol or amine compounds (14.1 mmol in 20 mL THF) dropwise for 45 minutes. The resulting reaction mixture was stirred at the same temperature for 3 hours. The reaction mixture was diluted with 0.5 M HCl (40 mL) and DCM (200 mL). The organic solution was washed with saturated NaHCO3 (200 mL) and brine (200 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting oil/solid was purified by flash chromatography over silica gel (EtOAc/hexanes) to give the pure product.
To a stirred and cooled (0° C.) solution of mono-substituted s-triazine compound (4.52 mmol) and N,N-diisopropylethylamine (1.05 mL, 5.88 mmol) in THF (15 mL) was added tert-butyl 2-aminoethylcarbamate (3) (796 mg, 4.97 mmol in 5 mL THF) dropwise for 15 minutes. After stirring at room temperature for 2 hours, the reaction mixture was diluted with 0.5 M HCl (10 mL) and DCM (100 mL). The organic solution was washed with saturated NaHCO3 (100 mL) and brine (100 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting oil/solid was purified by flash chromatography over silica gel (EtOAc/hexanes) to give pure product.
A mixture of compound containing ethylenediamine linker (10, 12, 14 and 16) (1 equivalent), compound 2b (1.5 equivalents) and N,N-diisopropylethylamine (2 equivalents) in acetonitrile was heated to reflux until ESI-MS analysis indicated disappearance of starting material (˜1 hour). The reaction mixture was diluted with 0.5 M HCl and DCM. The organic solution was washed with saturated NaHCO3 and brine. The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting oil/solid was purified by flash chromatography over silica gel (EtOAc/hexanes) to give pure product.
General Procedure for Deprotection of Boc Carbamate.
To a stirred and cooled (0° C.) solution of starting material in DCM (˜0.05-0.1 M solution) was added trifluoroacetic acid (TFA) (final concentration=50%) dropwise. After stirring at room temperature for 1 hour, the reaction mixture was diluted with DCM and was washed with water (2 times), saturated NaHCO3 (2 times) and brine. The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting oil/solid was used with any further purification.
According to general procedure for synthesis of mono-substituted s-triazine, compound 1a was prepared from ethanethiol (1.02 mL, 14.1 mmol). The resulting oil was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/25→1/20, v/v) to give 1a (2.69 g, 91%) as a colorless oil: Rf=0.29 (EtOAc/hexanes, 1/50, v/v); 1H NMR (500 MHz, CDCl3): δ 3.17 (q, J=8.3 Hz, 2H), 1.39 (t, J=7.5 Hz, 3H); 13C NMR (125 MHz, CDCl3): δ 186.67, 170.23, 25.90, 13.86; HRMS of 1a: m/z: calcd for C5H5Cl2N3S [M+H]+: 209.9654. found: 209.9653.
According to general procedure for synthesis of mono-substituted s-triazine, compound 1b was prepared from thiophenol (1.44 mL, 14.1 mmol). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/25→1/20, v/v) to give 1b (3.17 g, 87%) as an amorphous white solid: Rf=0.51 (EtOAc/hexanes, 1/20, v/v); 1H NMR (500 MHz, CDCl3): δ 7.51-7.48 (m, 2H), 7.46-7.39 (m, 3H); 13C NMR (125 MHz, CDCl3): δ 186.64, 170.60, 135.30, 130.82, 129.84, 125.58; HRMS of 1b: m/z: calcd for C9H5Cl2N3S [M+H]+: 257.9654. found: 257.9659.
According to general procedure for synthesis of mono-substituted s-triazine, compound 1c was prepared from 1-decanethiol (2.46 g, 14.1 mmol). The resulting oil was purified by flash chromatography over silica gel (EtOAc/hexanes, 25/1→20/1, v/v) to give 1c (4.04 g, 89%) as a colorless oil: Rf=0.31 (EtOAc/hexanes, 1/50, v/v). 1H NMR (500 MHz, CDCl3): δ 3.17-3.11 (m, 2H), 1.74-1.68 (m, 2H), 1.43-1.39 (m, 2H), 1.33-1.20 (m, 12H), 0.87-0.82 (m, 3H); 13C NMR (125 MHz, CDCl3): δ 186.77, 170.13, 32.03, 31.35, 29.65, 29.55, 29.43, 29.14, 28.79, 28.51, 22.82, 14.25; HRMS of 1c: m/z: calcd for C13H21Cl2N3S [M+H]+: 322.0906. found: 322.0918.
According to general procedure for synthesis of mono-substituted s-triazine, compound 1d was prepared from 4-chlorobenzenemethanethiol (1.41 mL, 14.1 mmol). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/25→1/20, v/v) to give 1d (3.89 g, 90%) as an amorphous white solid: Rf=0.41 (EtOAc/hexanes, 1/20, v/v); 1H NMR (500 MHz, CDCl3): δ 7.26-7.19 (m, 4H), 4.27 (s, 2H); 13C NMR (125 MHz, CDCl3): δ 185.45, 170.27, 133.99, 133.92, 130.75, 129.03, 34.93; HRMS of 1d: m/z: calcd for C10H6Cl3N3S [M+H]+: 305.9421. found: 305.9436.
According to general procedure for synthesis of mono-substituted s-triazine, compound 1e was prepared from aniline (1.3 mL, 14.1 mmol). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/20→1/9, v/v) to give 1e (3.09 g, 91%) as an amorphous white solid: Rf=0.32 (EtOAc/hexanes, 1/8, v/v); HRMS of 1: m/z: calcd for C9H6Cl2N4 [M+H]+: 241.0042. found: 241.0037.
To a stirred and cooled (0° C.) solution of ethylenediamine (26.7 mL, 400 mmol) in DCM (300 mL) was added di-tert-butyl dicarbonate (8.7 g, 40 mmol in 200 mL DCM) dropwise for 3 hours. After stirring at room temperature for 24 hours, the solvent was removed under reduced pressure. The crude product was dissolved in saturated NaHCO3 (100 mL) and was extracted with DCM (3×100 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure to give crude 3 (6.4 g, 100%) as a colorless oil. Compound 3 was used with any purification. HRMS of 3: m/z: calcd for C7H16N2O2 [M+H]+: 161.1285. found: 161.1277.
According to general procedure for synthesis of mono-substituted s-triazine, compound was prepared from β-alanine tert-butyl ester hydrochloride (2.56 g, 14.1 mmol, 20 mL acetone was added to dissolve this starting material). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/8 to 1/6, v/v) to give the product (3.11 g, 76%) as an amorphous white solid: Rf=0.27 (EtOAc/hexanes, 1/6, v/v); 1H NMR (500 MHz, CDCl3): δ 7.09 (t, J=7.5 Hz, 1H), 3.60 (q, J=6.7 Hz, 2H), 2.45 (t, J=5 Hz, 2H), 1.30 (s, 9H); 13C NMR (125 MHz, CDCl3): δ 171.02, 170.75, 169.68, 165.63, 81.40, 37.06, 34.36, 27.99; HRMS: m/z: calcd for C10H14Cl2N4O2 [M+H]+: 292.0494. found: 292.0477.
According to general procedure for synthesis of mono-substituted s-triazine, compound was prepared from tert-butyl 2-aminoethylcarbamate (2.26 g, 14.1 mmol). The resulting yellow solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/4 to 1/2, v/v) to give the product (3.87 g, 89%) as a pale yellow solid: Rf=0.27 (EtOAc/hexanes, 1/2, v/v); 1H NMR (500 MHz, CDCl3): δ 6.86 (s, 1H), 4.86 (s, 1H), 3.59-3.55 (m, 2H), 3.35 (broad s, 2H), 1.41 (s, 9H); 13C NMR (125 MHz, CDCl3): δ 171.09, 169.98, 166.22, 156.87, 80.33, 42.67, 39.91, 28.52; HRMS: m/z: calcd for C10H15Cl2N5O2 [M+H]+: 308.0676. found: 308.0662.
According to general procedure for synthesis of di-substituted s-triazine, compound 2a was prepared from mono-substituted compound 1c (1.46 g, 4.52 mmol). The resulting oil was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/3→1/4, v/v) to give 2a (1.78 g, 88%) as an amorphous white solid: Rf=0.31 (EtOAc/hexanes, 1/4, v/v); 1H NMR (500 MHz, CDCl3, a mixture of rotamers): δ 3.57-3.50 (m, 2H), 3.37-3.27 (m, 2H), 3.09-2.98 (m, 2H), 1.71-1.60 (m, 2H), 1.46-1.34 (m, 11), 1.31-1.19 (m, 12H), 0.88-0.83 (m, 3H); 13C NMR (125 MHz, CDCl3, a mixture of rotamers): δ 183.64, 182.24, 169.13, 167.83, 164.82, 164.37, 156.67, 156.26, 79.69, 79.55, 41.76, 40.08, 31.96, 30.63, 30.43, 29.62, 29.58, 29.57, 29.37, 29.26, 29.20, 29.08, 29.03, 28.94, 28.85, 28.45, 22.74, 14.18; HRMS of 2a: m/z: calcd for C20H36ClN5O2S [M+H]+: 446.2351. found: 446.2354.
According to general procedure for synthesis of di-substituted s-triazine, compound 2b was prepared from mono-substituted compound 2b (1.38 g, 4.52 mmol). The resulting crude product was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/3→1/2, v/v) to give 2b (1.77 g, 91%) as an amorphous white solid: Rf=0.45 (EtOAc/hexanes, 1/2, v/v); 1H NMR (500 MHz, CDCl3, a mixture of rotamers): δ 7.48-7.38 (m, 4H), 6.42 (broad s, 1H), 5.00 and 4.90 (two broad s, 1H), 4.43 and 4.39 (two s, 2H), 3.69-3.61 (m, 2H), 3.52-3.40 (m, 2H), 1.56 and 1.55 (two s, 9H); 13C NMR (125 MHz, CDCl3, a mixture of rotamers): δ 182.56, 181.26, 171.38, 169.29, 168.15, 164.85, 164.52, 156.90, 156.41, 135.60, 135.46, 133.39, 133.35, 130.59, 130.40, 128.85, 128.81, 79.99, 79.85, 60.57, 42.16, 41.85, 40.04, 34.23, 34.01, 28.48; HRMS of 2b: m/z: calcd for C17H21Cl2N5O2S [M+H]+: 430.0866. found: 430.0878.
In this example, triazine polymers are made using the synthetic methods illustrated below. The triazine polymers can be made to have more than three monomers and either can be homopolymers or heteropolymers.
A stirred solution of 2-nitrobenzaldehyde (1.98 g, 13.1 mmol) and tert-butyl 2-aminoethylcarbamate (3) (2.1 g, 13.1 mmol) in toluene (80 mL) was heated to 120° C. with Dean Stark apparatus for 5 hours. The reaction mixture was concentrated under reduced pressure to give crude 8 as a brown syrup. Crude compound 8 was dissolved in MeOH (80 mL) and sodium borohydride (510 mg, 13.5 mmol) was added in small portion. After stirring for 1 hour, the reaction mixture was diluted with acetone (20 mL). The solvent was removed under reduced pressure to give an amorphous brown solid. Without purification, this crude product was dissolved in MeOH (80 mL), and triethylamine (4 mL) and acetic anhydride (4 mL, excess) were added. After stirring for 2 hours, the reaction mixture was diluted with DCM (150 mL). The organic solution was washed with saturated NaHCO3 (150 mL), and brine (120 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting brown oil was purified by flash chromatography over silica gel (EtOAc/hexanes/DCM, 2/1/1, v/v) to give 9 (3.80 g, 86% over 3 steps) as an amorphous yellow solid: Rf=0.24 (EtOAc/hexanes, 5/2, v/v); 1H NMR (500 MHz, CDCl3, a mixture of rotamers): δ 8.14-7.96 (m, 1H), 7.65-7.53 (m, 1H), 7.48-7.36 (m, 1H), 7.27-7.26 (m, 1H), 4.94 and 4.89 (two s, 2H), 3.47-3.42 (m, 2H), 3.28-3.22 (m, 2H), 2.22 and 2.00 (two s, 3H), 1.38 (s, 9H); 13C NMR (125 MHz, CDCl3, a mixture of rotamers): δ 172.60, 171.90, 156.29, 156.10, 148.68, 147.86, 134.52, 133.82, 133.16, 128.79, 128.72, 128.20, 127.31, 126.05, 125.23, 80.02, 79.56, 51.06, 48.41, 46.48, 45.98, 39.38, 39.08, 28.52, 28.48, 21.68, 21.40; HRMS of 9: m/z: calcd for C16H23N3O5 [M+Na]+: 360.1530. found: 360.1522. According to general procedure for deprotection of Boc carbamate, compound 10 was prepared from compound 9 (398 mg, 1.18 mmol) in DCM (5 mL) and TFA (5 mL). HRMS of 10: m/z: calcd for C11H15N3O3 [M+H]+: 238.1186. found: 238.1179.
According to general procedure for synthesis of tri-substituted s-triazine, compound 11 was prepared from 10 (crude, 1.18 mmol), 2b (759 mg, 1.77 mmol) and N,N-diisopropylethylamine (0.4 mL, 2.36 mmol) in CH3CN (15 mL). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 2/1→MeOH/DCM, 1/30, v/v) to give 11 (655 mg, 88%) as an amorphous yellow solid: Rf=0.41 (MeOH/DCM, 1/20, v/v); 1H NMR (500 MHz, CDCl3, a mixture of rotamers): δ 8.17-7.96 (m, 1H), 7.68-7.53 (m, 1H), 7.53-7.35 (m, 1H), 7.32-7.15 (m, 5H), 5.03-4.91 (m, 2H), 4.28-4.17 (m, 2H), 3.66-3.40 (m, 6H), 3.35-3.22 (m, 2H), 2.21 and 2.04 (two s, 3H), 1.41 (s, 9H); 13C NMR (125 MHz, CDCl3, a mixture of rotamers): δ 172.66, 172.42, 164.83, 164.78, 164.66, 156.58, 156.46, 148.54, 147.84, 136.86, 136.65, 134.53, 133.86, 132.95, 132.87, 130.51, 130.41, 130.23, 128.83, 128.78, 128.72, 128.64, 128.16, 128.10, 127.34, 126.03, 125.97, 125.24, 125.14, 79.57, 79.29, 53.58, 50.98, 50.81, 48.62, 48.42, 46.39, 46.11, 41.10, 38.86, 38.75, 33.54, 33.24, 28.53, 21.64, 21.46; HRMS of 11: m/z: calcd for C28H35ClN8O5S [M+H]+: 631.2212. found: 631.2223.
According to general procedure for deprotection of Boc carbamate, compound 12 was prepared from compound 11 (436 mg, 0.692 mmol) in DCM (4 mL) and TFA (4 mL). HRMS of 12: m/z: calcd for C23H27ClN8O3S [M+H]+: 531.1688. found: 531.1671.
According to general procedure for synthesis of tri-substituted s-triazine, compound 13 was prepared from 12 (crude, 0.692 mmol), 2b (446 mg, 1.04 mmol) and N,N-diisopropylethylamine (0.18 mL, 1.38 mmol) in CH3CN (12 mL). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 2/1→MeOH/DCM, 1/30, v/v) to give 13 (595 mg, 93%) as an amorphous yellow solid: Rf=0.35 (MeOH/DCM, 1/20, v/v); 1H NMR (500 MHz, CDCl3, a mixture of rotamers): δ 8.14-7.93 (m, 1H), 7.65-7.48 (m, 1H), 7.48-7.32 (m, 1H), 7.31-7.12 (m, 9H), 4.93 (broad s, 2H), 4.28-4.10 (m, 2H), 3.63-3.18 (m, 12H), 2.17 and 1.99 (two broad s, 3H), 1.39 (s, 9H); 13C NMR (125 MHz, CDCl3, a mixture of rotamers): δ 172.06, 171.50, 164.79, 164.65, 164.58, 164.51, 156.46, 155.41, 147.77, 136.83, 136.82, 136.67, 136.60, 134.61, 134.56, 133.90, 133.01, 132.94, 130.49, 130.48, 130.30, 128.80, 128.70, 128.68, 128.13, 127.29, 126.02, 125.25, 79.59, 50.97, 50.82, 50.78, 46.20, 41.12, 40.90, 40.74, 33.50, 33.34, 28.57, 21.66, 21.45; HRMS of 13: m/z: calcd for C40H47Cl2N13O5S2 [M+H]+: 924.2714. found: 924.2677.
According to general procedure for deprotection of Boc carbamate, compound 14 was prepared from compound 13 (475 mg, 0.514 mmol) in DCM (4 mL) and TFA (4 mL). HRMS of 14: m/z: calcd for C35H39Cl2N13O3S2 [M+H]+: 824.2190. found: 824.2184.
Triazine polymer 15. According to general procedure for synthesis of tri-substituted s-triazine, compound 15 was prepared from 14 (crude, 0.514 mmol), 2b (330 mg, 0.771 mmol) and N,N-diisopropylethylamine (0.18 mL, 1.03 mmol) in CH3CN (12 mL). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 2/1→MeOH/DCM, 1/30, v/v) to give 15 (539 mg, 86%) as an amorphous yellow solid: Rf=0.31 (MeOH/DCM, 1/20, v/v); 1H NMR (500 MHz, CDCl3, a mixture of rotamers): δ 8.14-7.93 (m, 1H), 7.65-7.48 (m, 1H), 7.48-7.32 (m, 1H), 7.31-7.12 (m, 9H), 4.93 (broad s, 2H), 4.28-4.10 (m, 2H), 3.63-3.18 (m, 12H), 2.17 and 1.99 (two broad s, 3H), 1.39 (s, 9H); 13C NMR (125 MHz, CDCl3, a mixture of rotamers): δ 172.87, 164.65, 164.49, 164.31, 156.52, 156.47, 136.78, 136.73, 136.58, 134.70, 134.60, 133.90, 133.87, 133.02, 133.00, 132.98, 130.68, 130.63, 130.36, 128.75, 128.71, 127.34, 126.59, 126.10, 126.05, 125.33, 125.27, 79.78, 79.62, 69.72, 50.82, 46.24, 40.81, 40.43, 39.62, 33.61, 33.41, 31.94, 31.09, 29.86, 29.48, 28.59, 21.72, 21.67, 21.49, 21.47; HRMS of 15: m/z: calcd for C52H59Cl3N18O5S3 [M+H]+: 1217.3216. found: 1217.3255.
According to general procedure for deprotection of Boc carbamate, compound 16 was prepared from compound 15 (324 mg, 0.266 mmol) in DCM (3 mL) and TFA (3 mL). HRMS: m/z: calcd for C47H51Cl3N18O3S3 [M+H]+: 1117.2692. found: 1117.2747.
In this example, triazine polymers are made using the synthetic methods illustrated below. The triazine polymers can be made to have more than six monomers and either can be homopolymers or heteropolymers; linkers also can be modified within the polymer chain.
General Procedure for Submonomer Solid Phase Synthesis.
Triazine polymers were synthesized manually on Rink amide resin (60 mg, 0.046 mmol, loading: 0.77 mmol/g) involving a 2-step iterative process. Step 1: a mixture of mono-substituted s-triazine (10 equivalents), N,N-diisopropylethylamine (10 equivalents) and Rink amide resin was suspended in THF (1.2 mL). The resulting mixture was shaken at room temperature for 16 hours. The resin was filtered and washed successively with DCM and MeOH (3 times). Step 2: a mixture of ethylenediamine (20 equivalents), N,N-diisopropylethylamine (10 equivalents) and Rink amide resin was suspended in N-methyl-2-pyrrolidone (1.2 mL). The resulting mixture was shaken at 80° C. for 30 minutes to 5 hours. The resin was filtered and washed successively with DCM and MeOH (3 times). Steps 1 and 2 were repeated until desired polymer sequence was obtained. In some embodiments, a final step can be used to provide an amine terminal group or an amine side chain. Such a final step can include exposing the polymer to an ammonium hydroxide solution (20 equivalents) instead of ethylenediammine. The final product can be cleaved from the resin by the reaction with 50% TFA/DCM for 20 minutes.
To a stirred solution of cysteamine hydrochloride (5.41 g, 47.6 mmol) in DMF (75 mL) was added triphenylmethyl chloride (10.2 g, 36.6 mmol). After stirring at room temperature for 16 hours, the reaction mixture was diluted with 0.5 M HCl (100 mL) and DCM (400 mL). The organic solution was washed with saturated NaHCO3 (400 mL) and brine (300 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The product was used without any purification. HRMS: m/z: calcd for C21H21NS [M+H]+: 320.1467. found: 320.1466.
According to general procedure for synthesis of mono-substituted s-triazine, compound 3a was prepared from 2,4-dimethoxybenzylamine (2.36 g, 14.1 mmol). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/8 to 1/6, v/v) to give 3a (3.85 g, 87%) as an amorphous white solid: Rf=0.24 (EtOAc/hexanes, 1/6, v/v); HRMS of 3a: m/z: calcd for C12H12Cl2N4O2 [M+H]+: 315.0410. found: 315.0409.
According to general procedure for synthesis of mono-substituted s-triazine, compound 3b was prepared from 2-(tritylthio)ethanamine (4.51 g, 14.1 mmol). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/15 to 1/10, v/v) to give 3b (4.89 g, 75%) as an amorphous white solid: Rf=0.22 (EtOAc/hexanes, 1/10, v/v); HRMS of 3b: m/z: calcd for C24H20Cl2N4S [M+H]+: 467.0858. found: 467.0859.
According to general procedure for synthesis of mono-substituted s-triazine, compound 3c was prepared from ethanolamine (0.85 mL, 14.1 mmol). The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/4 to 1/2, v/v) to give 3c (2.12 g, 73%) as an amorphous white solid: Rf=0.29 (EtOAc/hexanes, 1/2, v/v); HRMS of 3c: m/z: calcd for C5H6Cl2N4O [M+H]+: 208.9991. found: 208.9967.
To a stirred solution of compound 3c (0.78 g, 3.77 mmol) in DMF (20 mL) was added 3,4-dihydro-2H-pyran (1.7 mL, 18.9 mmol) and TsOH.H2O (7.1 mg, 0.038 mmol). After stirring at room temperature for 24 hours, the reaction mixture was diluted with water (60 mL) and DCM (200 mL). The organic solution was washed with saturated NaHCO3 (200 mL) and brine (150 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/8 to 1/6, v/v) to give 3d (0.98 g, 89%) as an amorphous white solid: Rf=0.21 (EtOAc/hexanes, 1/6, v/v); HRMS: m/z: calcd for C10H14Cl2N4O2 [M+H]+: 293.0567. found: 293.0554.
Trimer 3e was synthesized according to the general submonomer solid phase synthesis method with monomer 1a. The crude product was purified by reversed phase HPLC on an semi-preparative C-18 column to give, after evaporation of solvents under reduced pressure, 3e (17 mg, 65%, based on initial loading of the resin) as an amorphous white solid: HRMS: m/z: calcd for C19H31N15S3[M+H]+: 566.2122. found: 566.2109.
Hexamer 3f was synthesized according to the general submonomer solid phase synthesis method (30 mg Rink amide resin) with monomer 3b. Deprotection of triphenymethyl group and cleavage of hexamer from resin were achieved by using a mixture triisopropylsilane/TFA/DCM (1/5/4) for 1 h. The crude product was purified by reversed phase HPLC on an semi-preparative C-18 column to give, after evaporation of solvents under reduced pressure, 3f (11 mg, 41%, based on initial loading of the resin) as an amorphous white solid: HRMS of 3f: m/z: calcd for C40H70N36S6 [M+H]+: 1247.4981. found: 1247.5028.
Trimer 3g was synthesized according to the general submonomer solid phase synthesis method with monomer 3a. Deprotection of 2,4-dimethoxybenzyl group and cleavage of trimer from resin were achieved by using pure TFA for 24 hrs. The crude product was purified by reversed phase HPLC on an semi-preparative C-18 column to give, after evaporation of solvents under reduced pressure, 3g (18 mg, 82%, based on initial loading of the resin) as an amorphous white solid: HRMS of 3g: m/z: calcd for C15H27N19 [M+H]+: 474.2770. found: 474.2783.
Hexamer 3h was synthesized according to the general submonomer solid phase synthesis method with monomer 3d. Deprotection of tetrahydropyranyl group and cleavage of trimer from resin were achieved by using MeOH/TFA/DCM (2/5/3) for 2h. The crude product was purified by reversed phase HPLC on an semi-preparative C-18 column to give, after evaporation of solvents under reduced pressure, 3h (31 mg, 58%, based on initial loading of the resin) as an amorphous white solid: HRMS of 3h: m/z: calcd for C40H70N36O6 [M+H]+: 1151.6352. found: 1151.6376.
Polymer 17 was made using the conditions disclosed above for Scheme 5 using the general submonomer solid phase synthesis method. The crude product was purified by reversed phase HPLC on an analytical C-18 column to give, after evaporation of solvents under reduced pressure, 17 (39 mg, 71%, based on initial loading of the resin) as an amorphous white solid: HRMS of 20: m/z: calcd for C42H69N31S6 [M+H]+: 1200.4749. found: 1200.4771.
According to the general procedure for submonomer solid phase synthesis, triazine polymer 18 was prepared using triazine compound 1d: 1H NMR (500 MHz, CDCl3): δ 7.35-7.06 (m, 24H), 4.30-3.95 (m, 12H), 3.61-3.32 (m, 22H), 3.03 (broad s, 2H); HRMS of 18: m/z: calcd for C72H75Cl6N31S6 [M+H]+: 1776.3350. found: 1776.3343.
According to the general procedure for submonomer solid phase synthesis, sequence-defined triazine polymer 19 was prepared using triazine compound 1a, 1b and 1d in a defined sequence. The crude product was purified by reversed phase HPLC on an analytical C-18 column to give, after evaporation of solvents under reduced pressure, 19 (48 mg, 75%, based on initial loading of the resin) as an amorphous white solid: 1H NMR (500 MHz, CDCl3): δ 7.58-7.10 (m, 14H), 4.17 (broad s, 2H), 3.62-3.27 (m, 22H), 3.10-2.87 (m, 8H), 1.22 (broad s, 9H); HRMS of 19: m/z: calcd for C55H70ClN31S6 [M+H]+: 1392.4516. found: 1392.4537.
Sequence defined polymer 4 was made using the general submonomer solid phase synthesis method with monomers described herein. The crude product was purified by reversed phase HPLC on an semi-preparative C-18 column to give, after evaporation of solvents under reduced pressure, 4 (19 mg, 31%, based on initial loading of the resin) as an amorphous white solid: 1H NMR (500 MHz, CDCl3): δ 7.32-7.15 (m, 4H), 4.26-4.15 (m, 2H), 3.72-3.40 (m, 24H), 3.16-2.87 (m, 8H), 2.52 (broad s, 2H), 1.29-1.18 (m, 6H); HRMS of 4: m/z: calcd for C49H74ClN35O4S3 [M+H]+: 1348.5586. found: 1348.5527.
In yet other embodiments, the following general procedure was used. Triazine polymers were synthesized manually on Rink amide resin (60 mg, 0.046 mmol, loading: 0.77 mmol/g) involving a 2-step process. Step 1: a mixture of mono-substituted s-triazine (10 equivalents), N,N-diisopropylethylamine (10 equivalents) and Rink amide resin was suspended in THF (1.2 mL). The resulting mixture was shaken at 35° C. for 1 hour. The resin was filtered and washed successively with DCM and MeOH (3 times). Step 2: a mixture of ethylenediamine (20 equivalents), N,N-diisopropylethylamine (10 equivalents) and Rink amide resin was suspended in N-methyl-2-pyrrolidone (1.2 mL). The resulting mixture was shaken at 80° C. for 30 minutes. The resin was filtered and washed successively with DCM and MeOH (3 times). Steps 1 and 2 were repeated until desired polymer sequence was obtained. The final product was cleaved from the resin by the reaction with 50% TFA/DCM for 20 minutes.
According to the general procedure for modified submonomer solid phase synthesis, compound 20 was prepared using triazine compound 1a. The crude product was purified by reversed phase HPLC on an analytical C-18 column to give, after evaporation of solvents under reduced pressure, 20 (23 mg, 81%, based on initial loading of the resin) as an amorphous white solid: HRMS of 20: m/z: calcd for C21H36N16S3 [M+H]+: 609.2544. found: 609.2537.
According to general procedure for synthesis of mono-substituted s-triazine, compound 1f was prepared from tert-Butyl 2-aminoethylcarbamate (2.26 g, 14.1 mmol). The resulting yellow solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 1/4→1/2, v/v) to give 1f (3.87 g, 89%) as a pale yellow solid: Rf=0.27 (EtOAc/hexanes, 1/2, v/v); HRMS of 1f: m/z: calcd for C10H15Cl2N5O2 [M+H]+: 308.0676. found: 308.0662.
According to the general procedure for modified submonomer solid phase synthesis, triazine polymer 21 was prepared using triazine compounds 1a and 1f. In this embodiment, the functionalized group present in compound if acts as a side chain and not as a linker group. The crude product was purified by reversed phase HPLC on an analytical C-18 column to give, after evaporation of solvents under reduced pressure, 21 as an amorphous white solid: HRMS of 21: m/z: calcd for C84H147N73S6 [M+2H]2+: 1186.1109. found: 1186.1066.
According to the general procedure for modified submonomer solid phase synthesis, triazine polymer 22 was prepared using triazine compounds 1d and 1f. The crude product was purified by reversed phase HPLC on an analytical C-18 column to give, after evaporation of solvents under reduced pressure, 22 as an amorphous white solid: 1H NMR (500 MHz, CDCl3): δ 7.31-7.05 (m, 24H), 4.25-4.08 (m, 12H), 3.72-3.32 (m, 58H), 3.15-2.90 (m, 14H); HRMS of 22: m/z: calcd for C114H153Cl6N73S6 [M+2H]2+: 1474.0409. found: 1474.0452.
According to general procedure for deprotection of Boc carbamate, compound 4 was prepared from di-substituted compound 2a (1.28 g, 2.87 mmol) in DCM (14 mL) and TFA (14 mL). HRMS of 2a: m/z: calcd for C20H36ClN5O2S [M+H]+: 446.2351. found: 446.2344.
To a stirred solution of crude 4 in N-methyl-2-pyrrolidone (28 mL) was added potassium tert-butoxide (644 mg, 5.74 mmol, 2 equivalents). The reaction mixture was heated to 120° C. for 16 hours. The reaction was added dropwise into water (120 mL) to remove excess potassium tert-butoxide and precipitate out the polymer. The crude product was collected by centrifugation, re-dissolved into N-methyl-2-pyrrolidone (20 mL) and re-precipitated by adding into water (200 mL). The resulting yellowish polymer 5 (470 mg, 53%) was collected by centrifugation and washed with water. GPC data: Mn=5330, Mw=6980, PDI=1.31.
To a stirred solution of 1e (1.00 g, 4.16 mmol) in acetone/H2O (3/1, v/v, 20 mL) were added 1,2-diaminopropane (177 μL, 2.08 mmol, in 2.2 mL acetone) and 2 M sodium hydroxide (2.2 mL, 4.4 mmol) simultaneously dropwise. After stirring for 5 hours, the reaction mixture was diluted with DCM (70 mL) and washed successively with 0.1M HCl (70 mL), water (70 mL) and brine (50 mL). The organic phase was dried (MgSO4), filtered and the filtrate was concentrated under reduced pressure. The resulting solid was purified by flash chromatography over silica gel (EtOAc/hexanes, 10/1→6/1, v/v) to give 6 (753 mg, 75%) as an amorphous white solid.
To a stirred and refluxing solution of 6 (120 mg, 0.248 mmol) in CH3CN (5 mL) were added a mixture of 1,2-diaminopropane (21 μL, 0.248 mmol) and Et3N (69 μL, 0.49 mmol) in CH3CN (0.5 mL) dropwise. The reaction mixture was refluxed 16 hours. The resulting pale yellow solid 7 (71 mg, 65%) was collected by centrifugation washed with water and THF.
In this embodiment, refluxing (66° C.) THF was used for the polymerization step. The polymer precipitated out of the reaction as a yellow solid and was washed with water. In this particular embodiment, the molecular weight of polymer 8 likely indicates that polymer may be a mix of tetramer to tetradecamers.
The presence of various side chains in the sequence defined triazine polymers 19 and 4 can be verified by using NMR spectrometry as shown in
Detailed simulation tools and methods are described below, starting with parameterizing structural features and generating force fields and generating partial charges; and ending with describing how favored conformations were identified from the simulation.
Simulation Compound 1, illustrated above is a model compound used for GAFF parameterization of TZPs. Ethylene diamine linker nitrogens are represented by the N-methyl side groups. The N-methyl groups are conjugated with the triazine ring, leading to restricted rotation of the ring/linker N bond.
Simulations of polymers with model triazine residues TSE (R═S-ethyl), TSP (R═S— pentyl) with ethylene diamine (EN2) linkers between residues were performed. As triazine polymer topologies and parameters are not present in standard MD forcefields, they were generated using the generalized amber forcefield (GAFF) and the programs ACPYp E and Antechamber. The AM1-BCC model was used to generate partial charges. For parameterizing the triazine core, linker nitrogens and S-aliphatic sidechains, Simulation Compound 1, 2,6-di-N-methyl,4-S-ethyl-triazine (TSE-NM2), was used. ACPYp E was also used to generate GROMACS-compatible topologies.
Replica-exchange implicit solvent simulations using GROMACS 4.6.4 were performed. First, polymers were constructed in extended starting conformations using an in-house python script. The AMBER03 forcefield was used with GAFF atom types and parameters added for triazines as necessary. The GBSA implicit solvent model with the Onufriev/Bashford/Case algorithm was used for calculating Born radii and a dielectric of 78.3, and infinite Van der Waals and Coulomb cutoffs. Non-periodic NVT simulations with velocity-rescale temperature coupling and a 0.2 ps coupling time were used.
Due to the high free energy barrier for rotating the bond between the linker nitrogen and the triazine ring, replica-exchange MD (REMD) was used to accelerate sampling. Prior to the replica exchange production run, the polymer was equilibrated for 200 ps at each temperature. For the production run, 16 replicas spanning 300 to 800K were used, with exchanges occurring every 1000 steps (2 ps). This resulted in a Metropolis acceptance rate of about 50% for the hexameric simulations. The simulations were performed for 500 ns at each temperature, saving conformations every 100 ps. The last 400 ns of the simulation at 300 K were used for analysis, resulting in an ensemble of 4000 conformers that were clustered. In some embodiments, 200 ps equilibration, simulations were run for 500 ns at each temperature with replica exchange every 2 ps during that 500 ns period of simulation. Data from the last 400 ns of the simulation at 300K were used to define a population of conformers, consisting of 4000 conformers, obtained once every 100 ps during the 400 ns period. This population of structures is called an ensemble.
For the explicit solvent simulations, the starting structure was solvated in a 1.5-nm thick layer of SPC water in a dodecahedral box and then 0.115 M KCl was added. 1.0-nm cutoffs for van der Waals and Coulombic interactions were used and the particle-mesh Ewald method was used for long-range electrostatic interactions. The simulations were performed in the NPT ensemble with a Parrinello-Rahman barostat with a 1 ps coupling time at 1 bar. A Nose-Hoover thermostat with 0.2 ps coupling time was applied separately to the polymer and to the solution (including ions). The simulations were performed for 100 ns with a timestep of 2 fs.
To identify favored conformations from the simulation, k-means clustering of the 4000 ensemble structures in R was performed, using the root mean square distance of the triazine rings as a distance metric. The parameter k, the number of clusters, is optimized by clustering using a range of of k and identifying the “knee” in a plot of F(k) vs. k, where F(k) is a “goodness of clustering” metric such as within-group sum of squares. @ @ A knee is a point at which the slope of F(k) vs. k greatly decreases.
For the goodness of clustering metric, the “order ratio,” that is, the fraction of the ensemble that is in a “compact” cluster with an “internal RMSD” of 0.15 nm or less was used. Internal RMSD was defined as the root mean square distance of all the pairs of structures in the cluster. Such compact clusters are most likely to represent stable conformations rather than unfolded or transient structures. The order ratio also gives an estimate of a given sequence's propensity to form stable, folded structures.
In some embodiments, parameters for the triazine residues and linkers were generated using the generalized amber forcefield (GAFF) and the programs ACPYp E and Ante-chamber, and the simulation was carried out for 500 ns using GROMACS 4.6.4. The conformer found (
Simulation of two all cis trimers interacting, revealed a more extended pattern of cross-strand paired hydrogen bonds (1.9-2.2 Å) and pi pi interactions (<4 Å). The conformer found, which represents 100% of population, is a nanorod with a repeating pattern of sidechains with a periodicity of 11-12 Å, ), where central rings on each strand are stabilized by two pairs of hydrogen bonds and a pi-pi interaction. In addition, as described in more detail in the SI, this type of structure is independently observed in folding of a related hexamer. When the nanorod structure is extended to a dimer of pentamers, it remains stable thereafter for 500 ns of explicit solvent simulations.
In view of the many possible embodiments to which the principles of the disclosed technology may be applied, it should be recognized that the illustrated embodiments are only preferred examples of the present disclosure and should not be taken as limiting the scope of the disclosure. Rather, the scope of the present disclosure is defined by the following claims. We therefore claim all that comes within the scope and spirit of these claims.
This application is a continuation of U.S. patent application Ser. No. 15/154,842, filed on May 13, 2016, which is a continuation-in-part of U.S. patent application Ser. No. 14/556,021, filed on Nov. 28, 2014, now U.S. Pat. No. 9,365,676, which claims priority to and the benefit of U.S. Provisional Application No. 62/045,499, filed on Sep. 3, 2014, each of which is incorporated herein by reference in its entirety.
This invention was made with Government support under Contract DE-AC05-76RL01830 awarded by the U.S. Department of Energy. The Government has certain rights in the invention.
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| 20170088671 A1 | Mar 2017 | US |
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| 62045499 | Sep 2014 | US |
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| Parent | 15154842 | May 2016 | US |
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| Parent | 14556021 | Nov 2014 | US |
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