Claims
- 1. A separation column for Matched Ion Polynucleotide chromatogaphy separation of polynucleotide fragments, the separation column having Matched Ion Polynucleotide Chromatography separation particles and said separation column having metal surfaces which have been passivated, coated or a combination thereof, wherein said surfaces are substantially free from multivalent cations which are free to interfere with polynucleotide separation.
- 2. The separation column of claim 1 wherein the column has a stainless steel, PEEK or titanium frit.
- 3. The separation column of claim 2 wherein the frit is a stainless steel frit.
- 4. The separation column of claim 2 wherein the frit is a titanium frit.
- 5. The separation column of claim 2 wherein the frit has been passivated by contacting the surfaces thereof with a member selected from the group consisting of nitric acid, phosphoric acid, and pyrophosphoric acid; coated or passivated by contacting the surfaces thereof with chelating agent; or a combination thereof.
- 6. The separation column of claim 5 wherein the frit has been passivated by subjecting it to an acid wash treatment.
- 7. The separation column of claim 5 wherein the frit has been passivated or coated by contacting the surface thereof with chelating agent.
- 8. The separation column of claim 7 wherein chelating agent includes a member selected from the group consisting of EDTA, Tiron, cupferron, 8-hydroxyquinoline, oxine and an iminodiacetic acid.
- 9. The separation column of claim 8 wherein chelating agent includes EDTA.
- 10. The separation column of claim 1 wherein the Matched Ion Polynucleotide Chromatography separation particles are silica particles.
- 11. The separation column of claim 10 wherein the column has a stainless steel, PEEK or titanium frit.
- 12. The separation column of claim 11 wherein the frit is a stainless steel frit.
- 13. The separation column of claim 11 wherein the frit is a titanium frit.
- 14. The separation column of claim 11 wherein the frit has been passivated by contacting the surfaces thereof with a member selected from the group consisting of nitric acid, phosphoric acid, and pyrophosphoric acid; coated or passivated by contacting the surfaces thereof with chelating agent; or a combination thereof.
- 15. The separation column of claim 14 wherein the frit has been passivated by subjecting it to an acid wash treatment.
- 16. The separation column of claim 14 wherein the frit has been coated or passivated by contacting the surface thereof with chelating agent.
- 17. The separation column of claim 16 wherein chelating agent includes a member selected from the group consisting of EDTA, Tiron, cupferron, 8-hydroxyquinoline, oxine and an iminodiacetic acid.
- 18. The separation column of claim 17 wherein chelating agent includes EDTA.
- 19. The column of claim 1 wherein said multivalent cations include Fe(III), Cr(III), or colloidal iron oxide.
- 20. A system for separating a mixture of polynucleotide fragments comprising a chromatographic separation column having two ends, said separation column containing Matched Ion Polynucleotide Chromatography separation particles held in the column between porous frits positioned at each end thereof, wherein the separation column has metal surfaces which have been passivated or coated wherein said surfaces are substantially free from multivalent cations which are free to interfere with polynucleotide separation.
- 21. The system of claim 20 wherein the column has a stainless steel, PEEK or titanium frit.
- 22. The system of claim 21 wherein the frit is a stainless steel frit.
- 23. The system of claim 21 wherein the frit is a titanium frit.
- 24. The system of claim 21 wherein the frit has been passivated by contacting the surfaces thereof with a member selected from the group consisting of nitric acid, phosphoric acid, and pyrophosphoric acid; coated or passivated by contacting the surfaces thereof with chelatingagent; or a combination thereof.
- 25. The system of claim 24 wherein the frit has been passivated by subjecting it to an acid wash treatment.
- 26. The system of claim 24 wherein the frit has been coated or passivated by contacting the surface thereof with chelating agent.
- 27. The system of claim 26 wherein chelating agent includes a member selected from the group consisting of EDTA, Tiron, cupferron, 8-hydroxyquinoline, oxine and an iminodiacetic acid.
- 28. The system of claim 27 wherein chelating agent includes EDTA.
- 29. The system of claim 20 wherein the Matched Ion Polynucleotide Chromatography separation particles am silica particles.
- 30. The system of claim 29 wherein the column has a stainless steel, PEEK or titanium frit.
- 31. The system of claim 30 wherein the frit is a stainless steel frit.
- 32. The system of claim 30 wherein the frit is a titanium frit.
- 33. The system of claim 30 wherein the frit has been passivated by contacting the surfaces thereof with a member selected from the group consisting of nitric acid, phosphoric acid, and pyrophosphoric acid; coated or passivated by contacting the surfaces thereof with chelating agent; or a combination thereof.
- 34. The system of claim 33 wherein the frit has been passivated by subjecting it to an acid wash treatment.
- 35. The system of claim 33 wherein the frit has been coated or passivated by contacting the surface thereof with chelating agent.
- 36. The system of claim 35 wherein chelating agent includes a member selected from the group consisting of EDTA, Tiron, cupferron, 8-hydroxyquinoline, oxine and an iminodiacetic acid.
- 37. The system of claim 36 wherein chelating agent includes EDTA.
- 38. A separation column for Matched Ion Polynucleotide Chromatography separation of polynucleotide fragments, the separation column containing matched Ion Polynucleotide Chromatography silica particles and having stainless steel, titanium, or PEEK frit surfaces which have been passivated, coated or a combination thereof wherein said surfaces are substantially free from multivalent cations which are free to interfere with polynucleotide separation.
- 39. A separation column of claim 38 wherein frit surfaces of the column have been passivated or coated by contact with chelating agent.
- 40. A system for separation a mixture of polynucleotide fragments comprising a chromatographic separation column having two ends, said separation column containing Matched Ion polynucleotide Chromatography silica particles held in the column between stainless steel, titanium or PEEK porous frits positioned at each end thereof, wherein the frit surfaces have been passivated or coated by contact with chelating agent, wherein said surfaces are substantially free from multivalent a cations which are free to interfere with polynucleotide separation.
RELATIONSHIP TO COPENDING APPLICATIONS
This is a division of Ser. No. 09/081,040, filed May 18, 1998, now U.S. Pat. No. 5,997,742, which, in turn, is a continuation-in-part application of Ser. No. 08/748,376, filed Nov. 13, 1996, now U.S. Pat. No. 5,772,889. This application is a regular U.S. patent application under 35 U.S.C. §111(a) and claims priority from the following, commonly assigned provisional applications, each filed under 35 U.S.C. §111(b): Ser. No. 60/049,123, filed Jun. 10, 1997; Ser. No. 60/063,835, filed Oct. 30, 1997; and Ser. No. 60/078,523, filed Mar. 18, 1998.
US Referenced Citations (3)
Provisional Applications (3)
|
Number |
Date |
Country |
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60/078523 |
Mar 1998 |
US |
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60/063835 |
Oct 1997 |
US |
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60/049123 |
Jun 1997 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
08/748376 |
Nov 1996 |
US |
Child |
09/081040 |
|
US |