Claims
- 20. (new) the method according to claim 17, wherein the first FCS measurement is repeated for different active ingredients or active ingredient combinations and is compared with the second FCS measurement.
- 21. (New) The method according to claim 18, wherein the time-dependent curve of the diffusion time is determined in the first and second FCS measurement.
- 22. (New) The method according to at claim 17, wherein a time-dependent curve is determined during the time-dependent determination by means of at least the first FCS measurement with different specimens successively.
- 23. (New) The method according to claim 17, wherein a first value is determined initially for the specimens by scanning different specimens and at least one additional value is determined for the specimens after scanning additional specimens.
- 24. (New) The method according to claim 17, wherein the measurement is carried out after the addition of the fluorescence-labeled substances.
- 25. (New) The method according to claim 17, wherein active ingredients such as paclitaxel, nocodazole, vinblastin, colchicine are examined.
- 26. (New) The method according to claim 17, wherein proteins such as tubulin, F-actin or tau protein are used as proteins capable of assembly.
- 27. (New) An arrangement for the examination of active ingredients which are provided for influencing intracellular processes, comprising:
a fluorescence correlation spectroscopy system; and means for carrying out a first fluorescence correlation measurement using said system of an assembly equilibrium of proteins that are capable of assembly by adding at least one active ingredient and fluorescence-labeled monomers and/or dimers.
- 28. (New) The arrangement according to claim 27, wherein a second FCS measurement of an assembly equilibrium is carried out by adding fluorescence-labeled monomers and/or dimers without active ingredients and the measurement values of the first and second measurements are compared.
- 29. (New) The arrangement according to claim 27, wherein said system is a microscopic arrangement for FCS measurement wherein specimen vessels in which the substances are pipetted are detected.
- 30. (New) The arrangement according to claim 27, wherein said microscopic arrangement is an inverted microscope.
- 31. (New) The arrangement according to claim 27, wherein the time-dependent curve of the diffusion time is determined in the FCS measurement.
- 32. (New) The arrangement according to claim 27, wherein an X/Y displacement unit is provided for detecting different specimen vessels.
- 33. (New) The arrangement according to claim 27, wherein the specimen vessels are temperature-regulated.
Priority Claims (1)
Number |
Date |
Country |
Kind |
100 13 854.3 |
Mar 2000 |
DE |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority of PCT Application Serial No. PCT/EP01/02922, filed Mar. 15, 2001 and German Application No. 100 13 854.3, filed Mar. 17, 2000, the complete disclosures of which are hereby incorporated by reference.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
PCT/EP01/02922 |
3/15/2001 |
WO |
|