SYSTEM AND METHOD FOR QUANTITATIVE MAGNETIZATION TRANSFER IMAGING BASED ON SPIN-LOCK

Abstract

Systems and methods for fast and robust quantification of magnetization transfer (MT) using off-resonance spin-lock MRI. The techniques can be insensitive to variations of the inherent relaxation rates R1 (1/T1) and R2 (1/T2) of the free-water pool and to variations of the chemical exchange pool. The techniques can also be robust in the presence of inhomogeneity in the B1 RF and/or B0 magnetic fields.

Description

BACKGROUND

This disclosure relates generally to magnetic resonance imaging (MRI) techniques, and in particular to techniques for quantitative magnetization transfer (MT) imaging based on spin-lock.

Magnetic resonance imaging (MRI) is a noninvasive diagnostic technique that can allow assessments of the composition and state of various tissues. In an MRI procedure, a patient is placed in a strong longitudinal magnetic field (B0) that aligns nuclear spins of atoms in the patient's body, producing a net magnetization vector. RF pulses with magnetic field components (B1) transverse to the longitudinal field and frequencies tuned to the Larmor frequency of an isotope of interest (often ¹H) are applied. These pulses can flip spins into a higher energy state, resulting in a transverse component to the magnetization vector. As these spins return to the ground state, responsive RF pulses from the patient's body can be detected. Based on the response to pulses, characteristics of the magnetization can be measured. Commonly used measurements include the spin-lattice relaxation time (T1), measurement of which is typically based on recovery of the longitudinal component of the magnetization vector, and the spin-spin relaxation time (T2), measurement of which is typically based on decay of the transverse component of the magnetization vector. Since different anatomical structures have different material compositions, quantification of T1 and/or T2 can provide information about the material composition of a structure being imaged, and particular pulse sequences can be optimized to quantify T1 or T2. Spin-lattice relaxation time in the rotating frame, known as T_1ρ (or R_1ρ=1/T_1ρ), is a magnetization characteristic that is sensitive to molecular interactions, including dipolar interactions, chemical exchange, and magnetization transfer. R_1ρ quantification is typically performed using spin-lock MRI. However, R_1ρ relaxation also occurs during off-resonance RF saturation.

In addition to providing high-quality anatomical images of tissues, MRI can be used to examine tissue at a molecular level. For instance, it is possible to measure magnetization transfer (MT) effects, in which magnetization is transferred between protons of mobile water (commonly referred to as the “free pool”) and protons associated with semi-solid macromolecules such as lipids and other complex molecules (commonly referred to as the “bound pool”). The protons of the bound pool have ultrashort T2 relaxation and therefore a significantly broader absorption lineshape compared to the mobile protons. Applying off-resonance saturation RF pulses during an MRI procedure allows protons of the bound pool to be selectively saturated while keeping the mobile protons unaffected. The saturation is transferred to the free-water pool due to dipolar interactions and chemical exchange, resulting in MT contrast.

MT ratio (MTR) is commonly used to characterize magnetization transfer. However, MTR is not an intrinsic tissue property because it is influenced by the pulse sequence parameters. Quantitative MT approaches have been developed to measure tissue-specific MT parameters based on a two-pool model having a free-water pool and a bound pool as described above. The model specifies parameters including T1 and T2 for each pool, magnetization exchange rates between the two pools, and the molar fraction of protons that are bound to macromolecules, also known as bound pool fraction (BPF) or macromolecular proton fraction (MPF). MPF is a tissue-specific parameter that is directly linked to the macromolecular density and composition and is independent of acquisition parameters.

In clinical applications, however, extracting MPF from Mill data is challenging, in part because of the need to quantify multiple MT parameters, which requires multiple Mill scans using different acquisition parameters and complicated post-processing. Acquisition time can be reduced by estimating only the most clinically relevant parameters, including MPF. Various techniques have been proposed. Some of these techniques use a single off-resonance RF saturation measurement, combined with assumptions that cross-relaxation is sufficiently fast and that a T1 map has already been acquired (e.g., using additional MRI scans). Another approach involves labeling the spins of the mobile protons rather than saturating off-resonance spins associated with the bound pool, using techniques such as stimulated echo amplitude modulation (STEAM) and fitting to a mono-exponential longitudinal relaxation model at steady state. This avoids the need to acquire a T1 map; however, stimulated echo techniques have intrinsically low signal-to-noise. Accordingly, faster and more accurate techniques for MPF quantification would be desirable.

SUMMARY

Certain embodiments of the present invention relate to systems and methods for fast and robust quantification of magnetization transfer (MT) using off-resonance spin-lock MRI.

The techniques can be insensitive to variations of the inherent relaxation rates R1 (1/T1) and R2 (1/T2) of the free-water pool and to variations of the chemical exchange pool. The techniques can also be robust in the presence of inhomogeneity in the B1 RF and/or B0 magnetic fields.

Some embodiments relate to methods for quantifying parameters of magnetization transfer using an Mill apparatus. Multiple image acquisition processes can be performed to produce a set of MRI images, where each image acquisition process (i) in the plurality of image acquisition process includes applying an off-resonance spin-lock pulse having an RF amplitude (ω₁⁽ⁱ⁾) and a frequency offset from resonance (Δω⁽ⁱ⁾). Based on the set of Mill images, parameters of magnetization transfer can be computed. In particular, a parameter R_mpfsl can be computed, where R_mpfsl is defined as R_mpfsl=R_1ρ⁽²⁾−R_1ρ⁽²⁾, where R_1ρ⁽¹⁾ is a first relaxation rate in the rotating frame responsive to a spin-lock pulse having a first RF amplitude ω₁⁽¹⁾ and a first frequency offset Δω⁽¹⁾; R_1ρ⁽²⁾ is a second relaxation rate in the rotating frame responsive to a spin-lock pulse having a second RF amplitude ω₁⁽²⁾ and a second frequency offset Δω⁽²⁾; and the first RF amplitude ω₁⁽¹⁾, the first frequency offset Δω⁽¹⁾, the second RF amplitude ω₁⁽²⁾, and the second frequency offset Δω⁽²⁾ are chosen such that Δω⁽¹⁾/ω₁⁽¹⁾=Δω⁽²⁾/ω₁⁽²⁾. Additional parameters of magnetization transfer can also be computed, such as: a macromolecular proton fraction (MPF) indicating a fraction of protons that are bound to semi-solid macromolecules; a pool size ratio (f_b) for a magnetization transfer pool; an exchange rate (k_ba) between the magnetization transfer pool and a free-water pool; and/or a transverse relaxation time of the magnetization transfer pool (T_2b).

In various embodiments, different image-acquisition approaches may be used to determine R_mprsl. For example, in some embodiments, a set of four images can be acquired, two images at each of two combinations of spin-lock parameters. For each combination of spin-lock parameters one image is acquired with a toggling RF pulse applied before the spin-lock pulse and one image is acquired without a toggling RF pulse. From the four images, relaxation parameter R_mprsl can be computed.

In other embodiments, a first image acquisition process uses a first spin-lock pulse at the first RF amplitude ω₁⁽¹⁾ and the first frequency offset Δω⁽¹⁾ with a spin-lock time of zero to determine a first magnetization value; a second image acquisition process uses a second spin-lock pulse at the first RF amplitude ω₁⁽¹⁾ and the first frequency offset Δω⁽¹⁾ with a spin-lock time greater than zero to determine a second magnetization value; and a third image acquisition process uses a third spin-lock pulse at the second RF amplitude ω₁⁽²⁾ and the second frequency offset Δω⁽²⁾ to determine a third magnetization value. Where an observed longitudinal relaxation rate (R_1obs) and an equilibrium magnetization (M₀) are known (or where the product R_1obs·M₀ is known), relaxation parameter R_mpfsl can be computed by computing the first relaxation rate R_1ρ⁽¹⁾ based on the first magnetization value and the second magnetization value and computing the second relaxation rate R_1ρ⁽²⁾ based on the first magnetization value and the Rip third magnetization value, then subtracting.

In other embodiments, the first subset of the image acquisition processes and the second subset of the image acquisition processes each include at least two image acquisition processes (so that the total number of image acquisition processes is at least four). Each image acquisition process in the first subset uses a spin-lock pulse having a time of spin-lock (TSL) that is different from the TSL of each other image acquisition process in the first subset, and each image acquisition process in the second subset uses a spin-lock pulse having a TSL that is different from the TSL of each other image acquisition process in the second subset. The first relaxation rate R_1ρ⁽¹⁾ and the second relaxation rate R_1ρ⁽²⁾ can be computed from the plurality of images by finding optimized solutions to equations characterizing the magnetization as a function of TSL. In some embodiments, the pulse sequence can also be designed to provide a predictable relationship between the initial magnetization at the beginning of spin-lock and the equilibrium magnetization.

The following detailed description, together with the accompanying drawings, provides a further understanding of the nature and advantages of the claimed invention.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows an MRI system that can be used in connection with practicing some embodiments of the present invention.

FIG. 2 is a graph showing results of approximate and exact computations of a relaxation parameter R_mpfsl for various tissue types and macromolecular proton fraction (MPF) according to some embodiments.

FIGS. 3A-3E show an example of the relationship between relaxation parameter R_mpfsl and RF amplitude of the spin-lock RF pulse (or saturation RF pulse), ω₁⁽¹⁾, also referred to as frequency of spin-lock (FSL), frequency offset Δω⁽¹⁾, and scaling factor N=Δω⁽²⁾/Δω⁽¹⁾, using parameters for liver, as computed using full-equation Bloch-McConnell simulations according to some embodiments. FIG. 3A shows an R_mpfsl map as a function of frequency of spin-lock (FSL) and frequency offset (FO). FIG. 3B shows the corresponding relative percentage error between the exact R_mpfsl and the approximate R_mpfsl. FIG. 3C shows the exact R_mpfsl and the approximate R_mpfsl as a function of FO under various conditions. FIG. 3D shows the exact R_mpfsl and the approximate R_mpfsl as a function of FSL under various conditions. FIG. 3E shows the exact R_mpfsl and the approximate R_mpfsl as a function of the scaling factor N=Δω⁽²⁾/Δω⁽¹⁾.

FIG. 4 shows a flow diagram of a first process for determining MPF according to some embodiments.

FIG. 5 shows a flow diagram of a second process for determining MPF according to some embodiments.

FIG. 6 shows a flow diagram of a third process 600 for determining MPF according to some embodiments.

FIG. 7 shows simulation results according to some embodiments for different tissue types and for varying values of different parameters.

FIG. 8 shows an example of a mismatch between expected and actual spin-lock direction that can occur due to field inhomogeneities.

FIGS. 9A-9C show results of a simulation of the effect of varying amounts of field inhomogeneities on the deviation between expected and actual spin-lock field direction (FIG. 9A), the angle between actual spin-lock field direction and magnetization direction (FIG. 9B), and the relative error in measured MPF (FIG. 9C) according to some embodiments.

DETAILED DESCRIPTION

Examples (also referred to as “embodiments”) of systems and methods that provide quantification of parameters of magnetization transfers are described in this section. According to these and other embodiments, parameters of magnetization transfer can be quantified using spin-lock magnetic resonance imaging (MRI) techniques. It is noted that spin-lock also occurs during saturation RF pulses; accordingly, in embodiments described herein, off-resonance spin-lock RF pulse clusters or saturation RF pulses can be applied, and the term “spin-lock pulse” is used to refer to both spin-lock pulse clusters and saturation RF pulses. A series of acquisitions can be performed to provide a final measurement that is specific to the MT pool and independent of the free-water pool and the chemical exchange pool. As will become apparent, embodiments described herein allow quantification of magnetization transfer without acquisition of a T1 map or any assumption about the relaxation properties of the free-water pool.

MRI Systems

FIG. 1 shows an MRI system that can be used in connection with practicing some embodiments of the present invention. MRI system 100 includes a computer 102 communicably coupled to an MRI apparatus 104.

Computer 102 can be of generally conventional design and can include a user interface 106, a processor 108, a memory 110, a gradient controller 112, an RF controller 114, and an RF receiver 116. User interface 106 can include components that allow a user (e.g., an operator of MRI system 100) to input instructions or data and to view information. For example, user interface 106 can include a keyboard, mouse, joystick, display screen, touch-sensitive display screen, and so on. Processor 108 can include a general purpose programmable processor (or any other processor or set of processors) capable of executing program code instructions to perform various operations. Memory 110 can include a combination of volatile and nonvolatile storage elements (e.g., DRAM, SRAM, flash memory, magnetic disk, optical disk, etc.). Portions of memory 110 can store program code to be executed by processor 108. Examples of the program code can include a control program 118, which can coordinate operations of MRI apparatus 104 as described below in order to acquire data, and an analysis program 120, which can perform analysis algorithms on data acquired from MRI apparatus 104 (e.g., as described below). Gradient controller 112, RF controller 114, and RF receiver 116 can incorporate standard communication interfaces and protocols to communicate with components of MRI apparatus 104 as described below.

MRI apparatus 104 can be of generally conventional design and can incorporate a magnet 130, a gradient coil 132, and RF coils 134, 136. Magnet 130 can be a magnet capable of generating a large constant magnetic field B0 (e.g., 1.5 T, 3.0 T, or the like) in a longitudinal direction, in a region where a patient (or other subject to be imaged) can be placed. Gradient coil 132 can be capable of generating gradients in the constant magnetic field B0; operation of gradient coil 132 can be controlled by computer 102 via gradient controller 112. RF coils 134, 136 can include a transmitter (TX) coil 134 and a receiver (RX) coil 136. In some embodiments, a single coil can serve as both transmitter and receiver. In some embodiments, RF transmitter coil 134 can be placed around the portion of the subject's body that is to be imaged while RF receiver coil 136 is placed elsewhere within MRI apparatus 104. The preferred placement of RF coils 134, 136 may depend on the specific portion of the body that is to be imaged; those skilled in the art with access to the present disclosure will be able to make appropriate selections.

In operation, computer 100 can drive gradient coil 132 using gradient controller 112 to shape the magnetic field around the region being imaged. Computer 100 can drive RF transmitter coil 134 using RF controller 114 to generate RF pulses at a desired frequency (e.g., a resonant frequency for an isotope of interest), driving nuclear spins into an excited state. RF receiver coil 136 can detect RF waves generated by the spins relaxing from the excited state when RF pulses are not being generated. RF receiver 116 can include amplifiers, digital-to-analog converters, and other circuitry to generate digital data from the RF waves detected by RF receiver coil 136. RF receiver 116 can provide this data to processor 108 for analysis.

MRI system 100 is illustrative, and many variations and modifications are possible. Those skilled in the art will be familiar with a variety of MRI apparatus and control systems and with basic principles of MRI data acquisition, including the use of gradient fields and RF pulses, as well as techniques for detecting signals responsive to RF pulses and processing those signals to generate images.

In some embodiments, MRI system 100 or other MRI apparatus can be used to generate pulse sequences suitable for MT imaging of a subject, such as a specific organ or tissue within a patient. Examples of pulse sequences and imaging operations are described below.

Typical MRI imaging processes include a “preparation” phase and an “acquisition” phase. During the preparation phase, various pulse sequences can be generated in RF transmitter coil 136 to create a desired state of the magnetization vectors of nuclei of interest. For instance, a “reset” sequence may be used to reset net magnetization such that net magnetization becomes zero. Other types of preparation can include pulse sequences designed to suppress signals from specific types of tissue not of interest (e.g., blood, fat). In embodiments described herein, the magnetization preparation sequence can include a spin-lock RF pulse cluster or a saturation RF pulse. A spin-lock RF pulse cluster consists of a tip-down RF pulse, a spin-lock RF pulse, and a tip-up RF pulse. A saturation RF pulse includes a spin-lock RF pulse without a tip-down RF pulse or tip-up RF pulse. Spin-lock can also occur during a saturation RF pulse, and the term “spin-lock pulse” is used herein to encompass a spin-lock RF pulse cluster or a saturation RF pulse (or other pulses or pulse clusters during which spin-lock occurs). The spin-lock RF pulse or saturation RF pulse is applied for a specified time duration (referred to as the time of spin-lock, or TSL). Some magnetization preparation sequences can also include a toggling RF pulse. After the preparation phase, acquisition can be performed using various sequences such as fast spin echo sequences or other sequences as desired.

MPF Quantification Process

Certain embodiments described herein perform MPF quantification. In some embodiments, a two-pool model is used, in which the measured relaxation rate is based on contributions from two different magnetization pools: a free-water pool (also referred to as “pool a”) and a pool of protons bound to macromolecules (also referred to as a “bound pool” or “pool b”). The model assumes that magnetization exchanges can occur between the free-water and bound pools at some rate. The fractional sizes of the two pools are initially unknown. The notation shown in Table 1 is used to denote various parameters of the two pools.

TABLE 1
R1a = 1/T1a Longitudinal relaxation rate of pool a (free-water pool)
R1b = 1/T1b Longitudinal relaxation rate of pool b (bound pool)
R2a = 1/T2a Transverse relaxation rate of pool a
R2b = 1/T2b Transverse relaxation rate of pool b
fa, fb      Pool size ratios of pools a and b (fa = 1)
kba, kab    Exchange rates between pool b and pool a

According to the two-pool model, for a spin-lock pulse having an RF amplitude ω₁ and resonance frequency offset Δω), the relaxation rate during spin-lock (R_1ρ) can be derived by solving the Bloch-McConnell equation:

R _1ρ(Δω,ω₁)=R_water(Δω,ω₁)+R_mt(Δω,ω₁)  (1)

where R_water is the effective relaxation rate of the water in the rotating frame and R_mt is the MT-dependent relaxation rate. The effective relaxation rate R_water can be expressed as:

R _water =R _1a cos²θ+R_2a sin²θ,  (2)

where

$\begin{array}{cc}{\sin }^2\theta =\frac{{\omega }_1^2}{{\omega }_1^2+\Delta {\omega }^2},{\cos }^2\theta =\frac{\Delta {\omega }^2}{{\omega }_1^2+\Delta {\omega }^2}.& \left(3\right)\end{array}$

The MT-dependent relaxation rate R_mt can be expressed as:

$\begin{array}{cc}{R}_{mt}=\frac{\left(\Delta {\omega }^2+r_{2a}^2\right)\left(k_{ba}{r}_{1a}+r_{1b}\left(k_{ab}+r_{1a}\right)\right)+{\omega }_1^2{r}_{2a}\left(k_{ba}+r_{1b}\right)}{\begin{array}{c}\left(\Delta {\omega }^2+r_{2a}^2\right)\left(k_{ab}+k_{ba}+r_{1a}+r_{1b}\right)+\\ 2r_{2a}\left(k_{ba}{r}_{1a}+r_{1b}\left(k_{ab}+r_{1a}\right)\right)+{\omega }_1^2\left(r_{2a}+k_{ba}+r_{1b}\right)\end{array}},& \left(4\right)\end{array}$

where r_1a=R_1a−R_water; r_2a=R_2a−R_water; and r_1b=R_1b+R_rfc−R_water, where R_rfc represents the saturation rate of the bound pool. The saturation rate of the bound pool R_rfc is a function of the RF amplitude (ω₁), off-resonance frequency (Δω), and T_2b. In some embodiments, R_rfc(Δω)=ω₁²πg_b(Δω), where

$\begin{array}{cc}{g}_b\left(\Delta \omega \right)=T_{2b}{\int }_0^1\frac{1}{3u^2-1}\sqrt{\frac{2}{\pi }}\exp \left(-2{\left(\frac{\mathrm{\Delta \omega }·T_{2b}}{3u^2-1}\right)}^2\right)du& \left(5\right)\end{array}$

is the super-Lorentzian lineshape for the bound pool. Other lineshapes, such as Gaussian lineshape, can also be used in some embodiments. A derivation of Eq. (4) as an analytical expression of MT-dependent relaxation based on the eigenspace solution of the Bloch-McConnell equation has been shown in Zaiss et al., “A combined analytical solution for chemical exchange saturation transfer and semi-solid magnetization transfer,” NMR in Biomedicine 28(2):217-30 (2015).

Macromolecular proton fraction (MPF) can be defined as:

$\begin{array}{cc}MPF=\frac{f_b}{1+f_b}.& \left(6\right)\end{array}$

According to some embodiments, MPF can be quantified by measuring the difference of R_1ρ from two determinations at two different combinations of off-resonance frequency and RF amplitude of the spin-lock pulse. (It should be understood that for off-resonance spin-lock, Δω≠0.) This parameter is defined as R_mpfsl. Specifically:

R _mpfsl =R _1ρ ⁽²⁾ −R _1ρ ⁽¹⁾ =R _1ρ(Δω⁽²⁾,ω₁⁽²⁾)−R_1ρ(Δω⁽¹⁾,ω₁⁽¹⁾)=ΔR_water+ΔR_mt,  (7)

where superscripts ⁽¹⁾ and ⁽²⁾ denote the different acquisitions. If the off-resonance frequencies and RF amplitudes for the two acquisitions satisfy the following condition:

Δω⁽¹⁾/ω₁⁽¹⁾=Δω⁽²⁾/ω₁⁽²⁾,  (8)

then Eq. (3) gives θ⁽¹⁾=θ⁽²⁾, and Eq. (2) yields ΔR_water=0.

In some embodiments, the off-resonance frequencies Δω⁽¹⁾ and Δω⁽²⁾ are chosen such that:

Δω⁽¹⁾/ω₁⁽¹⁾>>1  (9)

and

Δω⁽¹⁾>>R_2a.  (10)

For living tissue, R_2a is on the order of tens of Hertz. Under the conditions of Eqs. (9) and (10), r_1a≈0 and r_1c≈R_rfc. Thus, applying Eq. (4), Eq. (7) becomes:

$\begin{array}{cc}{R}_{mpfs1}=\Delta R_{mt}=k_{ba}^2{f}_b\left(1+f_b\right)\left(\frac{1}{\left(1+f_b\right)k_{ba}+R_{\mathrm{rfc}}^{\left(1\right)}}-\frac{1}{\left(1+f_b\right)k_{ba}+R_{\mathrm{rfc}}^{\left(2\right)}}\right).& \left(11\right)\end{array}$

FIG. 2 is a graph showing results of Eq. (11) (“approx”) compared to an “exact” R_mprsl computed using Eqs. (4), (7), and (8) without approximations, for three different tissue types: liver, cartilage, and white matter (wm) at different values of MPF. For purposes of the computations, MT parameters from previously published work were used. Parameter values for liver, cartilage, and white matter, respectively, were: T_1a=T_1b=812 ms, 1168 ms, and 1084 ms; T_2a=42 ms, 27 ms, and 69 ms; T_2b=7.7 μs, 8.3 μs, and 10 μs; f_b=6.9%, 17.1%, and 13.9%; k_ba=51 s⁻¹, 57 s⁻¹, and 23 s⁻¹. In all cases, the off-resonance spin-lock parameters for the two acquisitions were: Δω⁽¹⁾=2π·1000 Hz, Δω⁽²⁾=2π·4000 Hz, ω₁⁽¹⁾=2π·100 Hz, and ω₁⁽²⁾=2π·400 Hz. In all cases, Eq. (11) provides a very close approximation to an exact computation of R_mpfs1 across the range of MPF values. As shown in FIG. 2, R_mpfs1 increases monotonically with MPF, which is a function of f_b according to Eq. (6).

According to various embodiments, Eq. (11) forms the basis of an approach to MPF quantification. From Eqs. (6) and (11), it follows that MPF can be computed from a measurement of R_mprsl if the values of k_ba and T_2b are known. With regard to k_ba and T_2b, it has been shown that, for some applications, these parameters vary only within a narrow range from subject to subject. Accordingly, in some embodiments it can be assumed that k_ba and T_2b are constant, and published values can be used. Alternatively, k_ba and T_2b can be treated as variables, and MPF can be computed by fitting data from multiple acquisitions to Eq. (11). It is noted that a T1 map is not needed in order to extract MPF from measured R_mpfs1. Computing MPF from R_mpfsl may require a B1 map; however, a B1 map can be obtained quickly using conventional methods (or other methods that may be subsequently developed).

As noted above, the derivation of Eq. (11) assumes that the condition of Eq. (8) holds. Eq. (8) holds if Δω⁽²⁾=NΔω⁽¹⁾ and ω₁⁽²⁾=Nω₁⁽¹⁾ for a constant scaling factor N (which can be, but need not be, an integer). Thus, the RF amplitudes ω₁⁽¹⁾, ω₁⁽²⁾ and resonance frequency offsets Δω⁽¹⁾, Δω⁽²⁾ can be determined by selecting ω₁⁽¹⁾, Δω⁽¹⁾, and N. The particular values are a matter of design choice, which can be guided by the following considerations. First, the frequency offset for each acquisition should be large enough to satisfy Eqs. (9) and (10). Second, the derivation of Eq. (11) ignores the presence of a chemical exchange pool, which can contribute to R_1ρ and thus confound the measurement of R_mprsl. To avoid the confounding effect of chemical exchange, Δω can be chosen to be far away from the chemical shift of the chemical exchange pool. Third, the choice of ω₁⁽¹⁾, Δω⁽¹⁾, and N affects the signal level of R_mpfsl.

The particular relationship between R_mpfsl and a particular choice of coin, ω₁⁽¹⁾, ω₁⁽²⁾, Δω⁽¹⁾, Δω⁽²⁾ can be modeled using full-equation Bloch-McConnell simulations. FIGS. 3A-3E show an example of the relationship between R_mpfsl and spin-lock frequency ω₁⁽¹⁾ (denoted as FSL in FIGS. 3A-3D) and frequency offset Δω⁽¹⁾ (denoted as FO in FIGS. 3A-3E) according to some embodiments, using parameters for liver, as computed using full-equation Bloch-McConnell simulations. FIG. 3A shows an R_mpfsl map as a function of FSL and FO, for N=4. FIG. 3B shows the corresponding relative percentage error in decibels (dB) between the exact R_mpfsl and the approximate R_mpfsl. FIG. 3C shows the exact R_mpfsl and the approximate R_mpfsl as a function of FO for N=4 and FSL=50 Hz, 100 Hz, and 200 Hz, respectively. FIG. 3D shows the exact R_mpfsl and the approximate R_mpfsl as a function of FSL for N=4 and FO=500 Hz, 1000 Hz, and 2000 Hz, respectively. FIG. 3E shows the exact R_mpfsl and the approximate R_mpfsl as a function of scaling factor N for FSL=100 Hz and FO=500 Hz, 1000 Hz, and 2000 Hz, respectively.

Based on FIGS. 3A-3E, choice of the frequency offset Δω⁽¹⁾ (or FO) can be guided by observing that the error due to approximation rises when frequency offset is small, due to violation of Eqs. (9) and (10), as best seen in FIG. 3C. Accordingly, increased frequency offset helps to maintain accuracy of Eq. (11) when spin-lock frequency ω₁⁽¹⁾ (or FSL) increases; however higher frequency offset may lead to signal loss, as shown in FIG. 3B. Further, for a given frequency offset, an optimal spin-lock frequency can be defined as the spin-lock frequency that maximizes R_mpfsl at the given frequency offset (as shown in FIG. 3D). In clinical practice, the choice of spin-lock frequency FSL may also be constrained by the specific absorption rate (SAR) limit, as larger values may lead to disfavored high SAR deposition. Similarly, the choice of N can be guided by the observation that small N is preferred from an SAR perspective, while the R_mpfsl signal becomes small for small N (as shown in FIG. 3E). While liver tissue is used in this example, simulation studies of other tissue lead to similar considerations.

In some embodiments, these considerations lead to the following choice of parameter values: Δω⁽¹⁾=2π·1000 Hz ω₁⁽¹⁾=2π·100 Hz, and N=4. These parameter values are used in examples below; however, it should be understood that different parameter values may be chosen.

Acquisition Processes for R_mpfsl

As described above, MPF and other magnetization transfer parameters can be determined from one or more measurements of R_mpfsl, which is defined as the difference of R_1ρ from two data acquisitions at two different combinations of off-resonance frequency and RF amplitude of the spin-lock pulse. In some embodiments, R_1ρ can be measured using off-resonance spin-lock techniques in which a spin-lock RF pulse cluster is applied, followed by imaging data acquisition. A spin-lock RF pulse cluster can include a tip-down RF pulse, a spin-lock RF pulse, and a tip-up RF pulse. The tip-down RF pulse flips the longitudinal magnetization at a specific flip angle determined by the selected spin-lock frequency ω₁ and frequency offset Δω. After the tip-down pulse, the spin-lock RF pulse is applied for a duration in parallel with the magnetization and locks the spin at the specific flip angle. After the spin-lock RF pulse, the tip-up RF pulse flips the spin back to the longitudinal direction. Either hard pulses or adiabatic pulses can be used for the tip-down and tip-up RF pulses. In some embodiments, a saturation RF pulse can be used instead of a spin-lock RF pulse cluster. For saturation RF pulses, there is only a saturation (or spin-lock) RF pulse, with no tip-down or tip-up RF pulse. Where a saturation RF pulse is used, the time duration of the pulse may be much longer than the duration of the spin-lock RF pulse of a spin-lock RF pulse cluster, and the B1 amplitude of the saturation RF pulse may be much smaller than the B1 amplitude of the spin-lock RF pulse of a spin-lock RF pulse cluster. The term “spin-lock pulse” is used herein to encompass a spin-lock RF pulse cluster or a saturation RF pulse (or other pulses or pulse clusters during which spin-lock occurs).

Four approaches to measuring R_mpfsl will now be described. It should be understood that these approaches are illustrative and not limiting.

According to a first approach, R_mpfsl is obtained by a direct measurement, without separately measuring R_1ρ⁽¹⁾ and R_1ρ⁽²⁾. Direct measurement of R_mpfsl can involve combining a toggling RF pulse with an off-resonance spin-lock pulse (including a spin-lock RF pulse cluster or saturation RF pulse) to obtain magnetization-prepared images. Toggling RF pulses are described, e.g., in Alex K. Smith et al., “Rapid, High-Resolution Quantitative Magnetization Transfer Mill of the Human Spinal Cord,” NeuroImage 95:106-16, https://doi.org/10.1016/j.neuroimage.2014.03.005 (Jul. 15, 2014), and Tao Jin and Seong-Gi Kim, “Quantitative Chemical Exchange Sensitive Mill Using Irradiation with Toggling Inversion Preparation,” Magnetic Resonance in Medicine 68(4):1056-64, https://doi.org/10.1002/mrm.24449 (2012). The toggling RF pulse can be applied (or not) prior to the spin-lock pulse.

For example, four images can be acquired. Specifically, under each of two different conditions of spin-lock (Δω⁽¹⁾, ω₁⁽¹⁾) and (Δωo⁽²⁾, ω₁⁽²⁾) selected as described above, two images are obtained: a first image is obtained with the toggling RF pulse turned on (M_Tog⁽¹⁾ and M_Tog⁽²⁾), and a second image is obtained with the toggling RF pulse turned off (M_noTog⁽¹⁾ and M_noTog⁽²⁾). If the same duration of the spin-lock RF pulse or the saturation RF pulse (denoted as TSL) is used for all four acquisitions, then R_mpfsl can be computed as:

$\begin{array}{cc}{R}_{\mathrm{mpfsl}}=R_{1\rho }^{\left(2\right)}-R_{1\rho }^{\left(1\right)}=-\log \left(\frac{M_{Tog}^{\left(2\right)}-M_{noTog}^{\left(2\right)}}{M_{Tog}^{\left(1\right)}-M_{noTog}^{\left(1\right)}}\right)\text{/}TSL.& \left(12\right)\end{array}$

FIG. 4 shows a flow diagram of a process 400 for determining MPF according to some embodiments. Process 400 can be performed using an MRI apparatus such as MRI apparatus 100 of FIG. 1. At block 402, a subject (e.g., a patient whose tissue is to be imaged) is arranged within an MRI apparatus. This can include having the patient assume a supine or other desired position and aligning the patient within the MRI apparatus. In some embodiments, this may also include positioning of RF and/or gradient coils; the particular positioning will depend on what is being imaged.

At block 404, various preparatory pulse sequences can be applied. Examples include magnetization reset sequences, sequences to reduce the effect of selected tissue types (e.g., blood, fat, etc.), and the like. Such sequences can be conventional and are optional; a detailed description is omitted as not being critical to understanding the claimed invention.

At block 406, a first image acquisition is performed. In some embodiments, the first image acquisition can include applying a spin-lock pulse (including a spin-lock RF pulse cluster or a saturation RF pulse) with characteristics (Δωo⁽¹⁾, ω₁⁽¹⁾) at block 408, followed by signal acquisition at block 410. Signal acquisition can include generating RF pulses to stimulate a signal from the subject and operating an RF receiver coil to detect the signal. Various acquisition sequences can be performed, including single-shot or multi-shot fast spin echo (FSE) sequences; other acquisition sequences and techniques suitable for quantifying R1ρ can also be used. During the signal acquisition, a first data set can be collected. At block 412, a first image M_noTog⁽¹⁾ can be computed based on the data set. Conventional techniques for generating images from acquired MRI data can be used; examples include Fourier transform of acquired k-space data.

At block 416, a second image acquisition is performed. In some embodiments, the second image acquisition can include applying a toggling RF pulse at block 418, followed by applying a spin-lock pulse (including a spin-lock RF pulse cluster or a saturation RF pulse) with characteristics (Δωo⁽¹⁾, ω₁⁽¹⁾) at block 420, followed by signal acquisition at block 422 to collect a second data set. Signal acquisition operations can be similar or identical to the signal acquisition operations at block 412. At block 424, a second image M_Tog⁽¹⁾ can be computed based on the data set collected at block 422, similarly to computation of the first image at block 412.

At block 426, a third image acquisition is performed. The third image acquisition can be similar to the first image acquisition at block 406, except that the spin-lock pulse has different characteristics (Δω⁽²⁾, ω₁⁽¹⁾), and the third image can be identified as M_noTog⁽²⁾.

At block 436, a fourth image acquisition is performed. The fourth image acquisition can be similar to the second image acquisition at block 416, except that the spin-lock pulse has different characteristics (Δω⁽²⁾, ω₁⁽¹⁾) and the fourth image can be identified as M_Tog⁽²⁾.

At block 440, relaxation parameter R_mpfsl can be computed from the four images, e.g., according to Eq. (12). At block 442, MPF can be computed from R_mpfsl, e.g., according to Eqs. (11) and (6).

Process 400 is illustrative, and variations or modifications are possible. For instance, acquisition of the images can occur in any order. In this example, all four acquisitions use the same (nonzero) TSL, and TSL can be chosen as desired. Off-resonance frequencies and RF amplitudes for the spin-lock pulses (which can include a spin-lock RF pulse cluster or a saturation RF pulse) can be chosen as desired, subject to Eqs. (8)-(10). In some embodiments, crusher gradients can be applied after each spin-lock pulse and prior to each signal acquisition sequence. Fat suppression and/or other preparatory pulse sequences can be applied before or after each spin-lock pulse and prior to each signal acquisition sequence. Using process 400 or similar processes, R_mpfsl can be determined directly from measured magnetizations (or images), without separately measuring R_1ρ⁽¹⁾ and R_1ρ⁽²⁾.

According to a second approach, R_mpfsl can be determined by obtaining R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ individually, then computing the difference. In the second approach, it can be assumed that the observed longitudinal relaxation rate (R_1obs=1/T_1obs, where T_1obs is the observed T1 relaxation time) and equilibrium magnetization M₀ are known, or that the product R_1obs·M₀ is known. R_1obs and M₀ (or the product R_1obs·M₀) can be determined using conventional approaches (or other approaches subsequently developed), and a detailed description is omitted. Where R_1obs and M₀ are known or the product R_1obs·M₀ is known, R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ can be quantified using three acquisitions.

For instance, the first acquisition can use the following parameters: TSL=0 ms, Δω⁽¹⁾=2π·1000 Hz, and ω₁⁽¹⁾=2π·100 Hz. (It should be understood that when TSL=0 ms, Δω⁽¹⁾ and ω₁⁽¹⁾ determine the flip angle of the tip-down and tip-up RF pulses, and there is no spin-lock RF pulse.) The measured magnetization for the first acquisition (M¹) can be expressed as:

M ¹ =M _ini,  (13)

where M_ini is the initial magnetization after the tip-down RF pulse and at the beginning of the spin-lock RF pulse or saturation RF pulse.

For the second acquisition, the parameters can be: TSL=50 ms (or other non-zero time), ω₁⁽¹⁾=2π·100 Hz, and Δω⁽¹⁾=2π·1000 Hz. The measured magnetization at the end of spin-lock for the second acquisition (M²) can be expressed as:

$\begin{array}{cc}{M}^2=M_{ini}·e^{-R_{1\rho }^{\left(1\right)}·\mathrm{TSL}}+M_{SS1}·\left(1-e^{-R_{1\rho }^{\left(1\right)}·\mathrm{TSL}}\right),& \left(14\right)\end{array}$

where M_ss1 is the steady-state magnetization. M_ss1 can be expressed as:

$\begin{array}{cc}{M}_{ss1}=\frac{\mathrm{cos\theta }·R_{1obs}}{R_{1\rho }^{\left(1\right)}}{M}_0,& \left(15\right)\end{array}$

where θ=tan⁻¹(ω₁⁽¹⁾)/Δω⁽¹⁾). Assuming R_1obs and M₀ are known (or the product R_1obs·M₀ is known), it follows that after substituting Eqs. (13) and (15) into Eq. (14), R_1ρ⁽¹⁾, is the only unknown variable. Accordingly, R_1ρ⁽¹⁾ can be computed by solving Eq. (14).

For the third acquisition, the parameters can be: ω₁⁽²⁾=Nω₁⁽¹⁾ and Δω⁽²⁾=NΔω⁽¹⁾, where Nis a constant scaling factor. TSL can be the same as for the second acquisition or any other value greater than zero. The measured magnetization at the end of spin-lock for the third acquisition (M³) can be expressed as:

$\begin{array}{cc}{M}^3=M_{ini}·e^{-R_{1\rho }^{\left(2\right)}·\mathrm{TSL}}+M_{ss2}·\left(1-e^{-R_{1\rho }^{\left(2\right)}·\mathrm{TSL}}\right),& \left(16\right)\end{array}$

where M_ss2 is the steady-state magnetization. M_ss2 can be expressed as:

$\begin{array}{cc}{M}_{ss2}=\frac{\cos \theta ·R_{1obs}}{R_{1\rho }^{\left(2\right)}}{M}_0.& \left(17\right)\end{array}$

The same M_ini from Eq. (13) can be used if the same spin-lock preparation type (i.e., hard RF pulse or adiabatic) was used in the first and third acquisitions. Similarly to the second acquisition, after substituting Eqs. (13) and (17) into Eq. (16), R_1ρ⁽²⁾ is the only unknown variable. Accordingly, R_1ρ⁽²⁾ can be computed by solving Eq. (16).

Once R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ have been computed, R_mpfsl can be computed as R_mpfsl=R_1ρ⁽²⁾−R_1ρ⁽¹⁾ according to Eq. (7).

FIG. 5 shows a flow diagram of a process 500 for determining MPF according to some embodiments. Process 500 can be performed using an MRI apparatus such as MRI apparatus 100 of FIG. 1. At block 502, a subject (e.g., a patient whose tissue is to be imaged) is arranged within an MRI apparatus. This can include having the patient assume a supine or other desired position and aligning the patient within the MRI apparatus. In some embodiments, this may also include positioning of RF and/or gradient coils; the particular positioning will depend on what is being imaged.

At block 504, various preparatory pulse sequences can be applied. Examples include magnetization reset sequences, sequences to reduce the effect of selected tissue types (e.g., blood, fat, etc.), and the like. Such sequences can be conventional and are optional; a detailed description is omitted as not being critical to understanding the claimed invention.

At block 506, a first image acquisition is performed. In some embodiments, the first image acquisition can include applying a spin-lock pulse at block 508, with TSL=0 ms, Δω⁽¹⁾=2π·1000 Hz, and ω₁⁽¹⁾=2π·100 Hz, followed by signal acquisition at block 510. Similarly to process 400, signal acquisition can include generating RF pulses to stimulate a signal from the subject and operating an RF receiver coil to detect the signal. Various acquisition sequences can be performed, including single-shot or multi-shot fast spin echo (FSE) sequences; other acquisition sequences and techniques suitable for quantifying R1ρ can also be used. During the signal acquisition, a first data set can be collected. At block 512, a first image M¹ can be computed based on the data set. Conventional techniques for generating images from acquired MRI data can be used; examples include Fourier transform of acquired k-space data.

At block 516, a second image acquisition is performed. In some embodiments, the second image acquisition can include applying a spin-lock pulse (including a spin-lock RF pulse cluster or a saturation RF pulse) at block 520, with TSL=50 ms (or other non-zero time), ω₁⁽¹⁾=2π·100 Hz, and Δω⁽¹⁾=2π·1000 Hz, followed by signal acquisition at block 522 to collect a second data set. Signal acquisition operations can be similar or identical to the signal acquisition operations at block 510. At block 524, a second image M² can be computed based on the data set collected at block 522, similarly to computation of the first image at block 512.

At block 526, a third image acquisition is performed. In some embodiments, the third image acquisition can include applying a spin-lock pulse (including a spin-lock RF pulse cluster or a saturation RF pulse) at block 528 with TSL greater than zero, ω₁⁽²⁾=Nω₁⁽¹⁾, and Δω⁽²⁾=NΔω⁽¹⁾, where Nis a constant scaling factor, followed by signal acquisition at block 530 to collect a third data set. Signal acquisition operations can be similar or identical to the signal acquisition operations at block 510. At block 532, a third image M³ can be computed based on the data set collected at block 522, similarly to computation of the first image at block 512.

At block 534, R_1ρ⁽¹⁾ can be computed based on the first and second images, e.g., by solving Eq. (14) as described above. At block 536, R_1ρ⁽²⁾ can be computed based on the first and third images, e.g., by solving Eq. (16) as described above. At block 540 relaxation parameter R_mpfsl can be computed as R_mpfsl=R_1ρ⁽²⁾−R_1ρ⁽¹⁾. At block 542, MPF can be computed from R_mpfsl, e.g., according to Eqs. (11) and (6).

Process 500 is illustrative, and variations or modifications are possible. For instance, acquisition of the images can occur in any order. Off-resonance frequencies and RF amplitudes for the spin-lock pulses (which can include a spin-lock RF pulse cluster or a saturation RF pulse) can be chosen as desired, subject to Eqs. (8)-(10). In some embodiments, crusher gradients can be applied after each spin-lock RF pulse cluster or saturation RF pulse and prior to each signal acquisition sequence. Fat suppression and/or other preparatory pulse sequences can be applied before or after each spin-lock RF pulse cluster or saturation RF pulse and prior to each signal acquisition sequence. Process 500 uses three acquisitions rather than four but assumes that R_1obs and M₀ are known (or that the product R_1obs M₀ is known), which may require one or more additional acquisitions.

According to a third approach, R_mpfsl can be determined by obtaining R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ individually, then computing the difference, but without knowledge of R_1obs and M₀. For example, according to Eqs. (14) and (15), for an image acquisition i using RF amplitude ω₁⁽¹⁾ and frequency offset Δω⁽¹⁾, magnetization after spin-lock (M_i⁽¹⁾) can be expressed as:

$\begin{array}{cc}{M}_i^{\left(1\right)}=M_{ini}·e^{-R_{1\rho }^{\left(1\right)}·{\mathrm{TSL}}_i^{\left(1\right)}}+\frac{\cos \theta ·R_{1obs}}{R_{1\rho }^{\left(1\right)}}·M_0·\left(1-e^{-R_{1\rho }^{\left(1\right)}·{\mathrm{TSL}}_i^{\left(1\right)}}\right),& \left({14}^{\prime }\right)\end{array}$

where TSL_i⁽¹⁾ is the time of spin-lock for the ith acquisition. Similarly, according to Eqs. (16) and (17), for an image acquisition i using RF amplitude ω₁⁽²⁾ and frequency offset Δω⁽²⁾, magnetization after spin-lock can be expressed as:

$\begin{array}{cc}{M}_i^{\left(2\right)}=M_{ini}·e^{-R_{1\rho }^{\left(2\right)}·{\mathrm{TSL}}_i^{\left(2\right)}}+\frac{\cos \theta ·R_{1obs}}{R_{1\rho }^{\left(2\right)}}·M_0·\left(1-e^{-R_{1\rho }^{\left(2\right)}·{\mathrm{TSL}}_i^{\left(2\right)}}\right),& \left({16}^{\prime }\right)\end{array}$

where TSL_i⁽²⁾ is the time of spin-lock for the ith acquisition. In Eqs. (14′) and (16′), θ=tan⁻¹(ω₁⁽¹⁾/Δω⁽¹⁾) is known. TSL_i⁽¹⁾ or TSL_i⁽²⁾ is also chosen for each acquisition and therefore known, and M_i⁽¹⁾ or M_i⁽²⁾ is determined from acquired signal. Accordingly, Eqs. (14′) and (16′) involve four unknowns: M_ini, R_1ρ⁽¹⁾, R_1ρ⁽²⁾, and the product M₀·R_1obs. If at least two acquisitions i are performed using RF amplitude ω₁⁽¹⁾ and frequency offset Δω⁽¹⁾ and at least two other acquisitions i are performed using RF amplitude ω₁⁽²⁾ and frequency offset Δω⁽²⁾, with varying values of TSL, R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ can be derived from Eqs. (14′) and (16′) using traditional optimization approaches, neural networks, or other optimization procedures. R_mpfsl can be computed as R_mpfsl=R_1ρ⁽²⁾−R_1ρ⁽¹⁾.

FIG. 6 shows a flow diagram of a process 600 for determining MPF according to some embodiments. Process 600 can be performed using an MRI apparatus such as MRI apparatus 100 of FIG. 1. At block 602, a subject (e.g., a patient whose tissue is to be imaged) is arranged within an MRI apparatus. This can include having the patient assume a supine or other desired position and aligning the patient within the MRI apparatus. In some embodiments, this may also include positioning of RF and/or gradient coils; the particular positioning will depend on what is being imaged.

At block 604, various preparatory pulse sequences can be applied. Examples include magnetization reset sequences, sequences to reduce the effect of selected tissue types (e.g., blood, fat, etc.), and the like. Such sequences can be conventional and are optional; a detailed description is omitted as not being critical to understanding the claimed invention.

At block 606, at least four image acquisitions are performed. In some embodiments, each image acquisition i includes selecting characteristics for a spin-lock pulse (including a spin-lock RF pulse cluster or a saturation RF pulse) at block 608. The selected characteristics can include the duration of the spin-lock RF pulse or the saturation RF pulse (TSL_i), B1 amplitude of the spin-lock RF pulse (spin-lock frequency) or the saturation RF pulse, and frequency offset. In some embodiments, half the acquisitions are performed using a first selection of ω₁⁽¹⁾ and Δω⁽¹⁾ while the other half are performed using a second selection of ω₁⁽²⁾ and Δω⁽²⁾ that satisfy the conditions of Eqs. (8)-(10), while TSL_i is varied for different acquisitions such that different acquisitions i using the same selection of (ω₁⁽¹⁾, Δω⁽¹⁾) or (ω₁⁽²⁾, Δω⁽²⁾) use different TSL_i. At block 610, a spin-lock pulse having the selected characteristics is applied, followed by signal acquisition at block 612. Similarly to process 400 or process 500, signal acquisition can include generating RF pulses to stimulate a signal from the subject and operating an RF receiver coil to detect the signal. Various acquisition sequences can be performed, including single-shot or multi-shot fast spin echo (FSE) sequences; other acquisition sequences and techniques suitable for quantifying R_1ρ can also be used. During the signal acquisition, a data set can be collected. At block 614, an image M can be computed based on the data set. Conventional techniques for generating images from acquired MRI data can be used; examples include Fourier transform of acquired k-space data. Blocks 608-614 can be repeated for each image that is to be acquired. The number of image acquisitions can be chosen as desired. At least four acquisitions are needed since there are four unknowns to be determined. Additional acquisitions may be performed if desired and may improve the optimization result.

At block 620, after acquiring the images, R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ can be computed based on the images, using optimization procedures as described above. At block 622 relaxation parameter) R_mpfsl can be computed as R_mpfsl=R_1ρ⁽²⁾−R_1ρ⁽¹⁾. At block 624, MPF can be computed from R_mpfsl, e.g., according to Eqs. (11) and (6).

Process 600 is illustrative, and variations or modifications are possible. For instance, acquisition of the images can occur in any order. Off-resonance frequencies and RF amplitudes for the spin-lock pulses (which can include a spin-lock RF pulse cluster or a saturation RF pulse) can be chosen as desired, subject to Eqs. (8)-(10). In some embodiments, crusher gradients can be applied after each spin-lock pulse and prior to each signal acquisition sequence. Fat suppression and/or other preparatory pulse sequences can be applied before or after each spin-lock pulse and prior to each signal acquisition sequence. Process 600 can be performed using as few as four acquisitions.

According to a fourth approach, R_mpfsl can be determined by obtaining R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ individually, then computing the difference, again without knowledge of R_1obs and M₀. In the fourth approach, the pulse sequence is designed such that:

M _ini =M ₀ −M ₀ ·e ^{−τ1·R 1obs}   (18)

where τ1 is a known time interval. Considering Eqs. (14′), (16′), and (18), there are again four unknowns: R_1ρ⁽¹⁾, R_1ρ⁽²⁾, M₀ and R_1obs. Thus, as in the third approach, if at least two acquisitions i are performed using RF amplitude ω₁⁽¹⁾ and frequency offset Δω⁽¹⁾ and at least two other acquisitions i are performed using RF amplitude ω₁⁽²⁾ and frequency offset Δω⁽²⁾, with varying values of TSL_i, R_1ρ⁽¹⁾ and R_1ρ⁽²⁾ can be derived from Eqs. (14′), (16′), and (18) using traditional optimization approaches, neural networks, or other optimization procedures. R_mpfsl can be computed as R_mpfsl=R_1ρ⁽²⁾−R_1ρ⁽¹⁾. Process 600 or a similar process can be used to implement the fourth approach as well as the third approach.

Processes described above or other similar processes can provide a quantitative measurement of MPF that is specific to the MT effect. As shown in Eq. (7), the subtraction process can remove R_water and thus mitigate the influence of water content due to inflammation or other causes (e.g., iron in the liver). As noted above, these and other processes can use multiple images from which parameters f_b, k_ba, and T_2b can all be determined by fitting to the measurements using Eq. (11).

Examples: Simulation Studies

Numerical simulations have been performed applying process 400 to tissues including cartilage, white matter (WM), and liver. Nominal values for relevant parameters were defined as shown in Table 2. The spin-lock parameters were chosen as: Δω⁽¹⁾=2π·1000 Hz, Δω⁽²⁾=2π·4000 Hz, ω₁⁽¹⁾=2π·100 Hz, ω₁⁽²⁾=2π·400 Hz. Following off-resonance spin-lock, a crusher gradient was applied to dephase magnetization in the transverse plane prior to data acquisition.

TABLE 2
Tissue	T1a	T2a	T1b	T2b	kba	fb
Type	(ms)	(ms)	(ms)	(us)	(S−1)	(%)
Cartilage	1168	27	1168	8.3	57	17.1
WM	1084	69	1084	10	23	13.9
Liver	812	42	812	7.7	51	6.9

A first simulation study was performed to assess sensitivity of R_mprsl to the presence of chemical exchange. Eq. (11) was derived based on a two-pool model including a free-water pool and a bound pool. It is recognized that a third pool is typically present in tissues, representing chemical exchange. To assess sensitivity of R_mprsl to the presence of a chemical exchange (CEST) pool, simulations were carried out using a three-pool Bloch-McConnell simulation, with a free-water pool, CEST pool, and bound pool. For the CEST pool, the following parameters were used: R₁^cest=R_1a (longitudinal relaxation rate of the free-water pool); R₂^cest=67 Hz; chemical shift Δω_cest=1.9 ppm; exchange rate from CEST pool to free-water pool k^cest=1500 s⁻¹; and CEST pool size ratio f^cest=0.14%. For the free-water pool and the bound pool, parameters of Table 2 were used as nominal values. FIG. 7 shows simulation results for liver (blue circles), cartilage (red diamonds), and white matter (gold squares) for varying values of the following parameters: R_1a (graph 701); R_2a (graph 702); R_1b (graph 703); T_2b (graph 704); k_ba (graph 705); MPF, which is a function of f_b according to Eq. (6) (graph 706); R₂^cest (graph 707); k^cest (graph 708); and CEST pool size ratio f^cest (graph 709). In each graph 701-709, R_mpfsl is plotted as a function of the respective parameter over a range from half the nominal value of the parameter to twice the nominal value. For each tissue type, R_mpfsl is sensitive to f_b (MPF), k_ba, and T_2b, as predicted by Eq. (11), but is not sensitive to other parameters. It is noted that other work in the field has established that R_2b (=1/T_2b) and k_ba can be considered approximately constant in certain applications, which simplifies the process of determining MPF from R_mpfsl.

A second simulation study was performed to assess sensitivity of R_mpfsl to B1 RF and B0 field inhomogeneities. Assuming the condition of Eq. (8) is satisfied, θ⁽¹⁾=θ⁽²⁾, and the influence of the free-water pool is removed. However, the presence of B1 RF and B0 field inhomogeneities may result in θ⁽¹⁾≠θ⁽²⁾, resulting in calculation error. FIG. 8 shows an example of a mismatch between expected and actual spin-lock direction that can occur due to field inhomogeneities. In the ideal case, the direction of magnetization (M_ideal) aligns with the effective direction of spin-lock field (B_{1eff, ideal}) at an angle θ to the longitudinal (z) axis. In the presence of B1 RF and B0 field inhomogeneities, the actual effective direction of spin-lock field (B_{leff, real}) deviates from the ideal direction by an angle ϕ₂, and the direction of magnetization (M_real) deviates from the direction B_leff, real by an angle ϕ₁.

To assess the effect of field inhomogeneities, simulations were carried out using a two-pool Bloch-McConnell simulation, with a free-water pool and an MT pool, under varying conditions of B1 RF and B0 field inhomogeneities. Parameters for liver tissue were used. Under each set of conditions, determination of R_mpfsl and MPF was performed using the first approach described above. FIGS. 9A-9C show results of the simulation. FIGS. 9A and 9B show the distribution of angles ϕ₂ and ϕ₁, respectively, as a function of inhomogeneity in B1 (range from 0.7 to 1.3) and B0 (range from 100 Hz to +100 Hz). FIG. 9C shows the relative error in the measured MPF as a function of inhomogeneity in B1 and B0. As can be seen, the errors were less than 1% within the simulated range of inhomogeneities. Simulations using other tissue types (cartilage and white matter) yield similarly low relative error.

Additional Embodiments

While the invention has been described with reference to specific embodiments, those skilled in the art will appreciate that numerous modifications are possible. For example, pulse sequence parameters described above can be modified, and additional pulse sequences can be incorporated as desired. Any of the above or other approaches can be used to determine R_mpfsl, as defined by Eq. (7), from MRI image data. MT quantification as described herein can be applied to a variety of tissue types, not limited to specific examples disclosed herein.

In some embodiments, image analysis operations as described above can be performed in the same computer system that performs image acquisition (e.g., as described with reference to FIG. 1). In other embodiments, distributed computing systems can be used, and image data acquired using an image acquisition system (e.g., as described above with reference to FIG. 1) can be transferred to a different computer system for analysis. It should be understood that a computer systems can include hardware components of generally conventional design (e.g., processors, memory and/or other storage devices, user interface components, network interface components) and that program code or other instructions can be provided to the computer system to cause the system to perform computations and/or other processes implementing embodiments described herein or aspects thereof.

Thus, although the invention has been described with respect to specific embodiments, it will be appreciated that the invention is intended to cover all modifications and equivalents within the scope of the following claims.

Claims

1. A method for quantifying magnetization transfer using a magnetic resonance imaging (MRI) apparatus, the method comprising: performing a plurality of image acquisition processes to produce a plurality of Mill images, wherein each image acquisition process (i) in the plurality of image acquisition process includes applying an off-resonance spin-lock pulse having an RF amplitude (ω1(i)) and a frequency offset (Δω(i); andcomputing, based on the plurality of MRI images one or more parameters of magnetization transfer, wherein the one or more parameters of magnetization transfer include a parameter Rmpfsl defined as Rmpfsl=R1ρ(2)−R1ρ(1), wherein: R1ρ(1); is a first relaxation rate in the rotating frame responsive to a spin-lock pulse having a first RF amplitude ω1(1) and a first frequency offset Δω(1);R1ρ(2) is a second relaxation rate in the rotating frame responsive to a spin-lock pulse having a second RF amplitude ω1(2) and a second frequency offset Δω(2); andthe first RF amplitude ω1(1), the first frequency offset Δω(1), the second RF amplitude ω1(2), and the second frequency offset Δω(2) are chosen such that Δω(1)/ω1(1)=Δω(2)/ω1(2).

2. The method of claim 1 further comprising: computing one or more additional parameters of magnetization transfer based on the parameter Rmpfsl.

3. The method of claim 2 wherein the one or more additional parameters of magnetization transfer includes a pool size ratio (fb) of a bound pool of protons bound to semi-solid macromolecules that is computed from the parameter Rmpfsl according to the equation

4. The method of claim 3 wherein the saturation rate parameter Rrfc is a function of RF amplitude (ω1), off-resonance frequency (Δω), and a transverse relaxation time of the bound pool (T2b).

5. The method of claim 4 wherein the magnetization exchange rate kba and the transverse relaxation time of the bound pool T2b are treated as constants that do not depend on the off-resonance frequency Δω or the RF amplitude ω1.

6. The method of claim 4 wherein the magnetization exchange rate kba and the transverse relaxation time of the bound pool T2b are treated as variables that are determined by fitting to the plurality of MRI images.

7. The method of claim 2 wherein the one or more additional parameters of magnetization transfer include a macromolecular proton fraction (MPF) indicating a fraction of protons that are bound to semi-solid macromolecules.

8. The method of claim 1 wherein performing the plurality of image acquisition processes includes: performing a first image acquisition process using a first spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) without a toggling RF pulse, wherein the first image acquisition process produces a first MRI image (MnoTog(1));performing a second image acquisition process using a second spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) with a toggling RF pulse, wherein the second image acquisition process produces a second MRI image (MTog(1));performing a third image acquisition process using a third spin-lock pulse at the second RF amplitude ω1(1) and the second frequency offset Δω(2) without a toggling RF pulse, wherein the third image acquisition process produces a third MRI image (MnoTog(2)); andperforming a fourth image acquisition process using a fourth spin-lock pulse at the second RF amplitude ω1(2) and the second frequency offset Δω(2) with a toggling RF pulse, wherein the fourth image acquisition process produces a fourth MRI image (MTog(2)).

9. The method of claim 8 wherein a same time of spin-lock (TSL) is used for each of the first, second, third, and fourth spin-lock pulses and wherein the relaxation parameter Rmpfsl is measured according to the equation:

10. The method of claim 1 wherein: performing the plurality of image acquisition processes includes: performing a first image acquisition process using a first spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) with a spin-lock time of zero to determine a first magnetization value;performing a second image acquisition process using a second spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) with a spin-lock time greater than zero to determine a second magnetization value;performing a third image acquisition process using a third spin-lock pulse at the second RF amplitude with ω1(2) and the second frequency offset Δω(2) with a spin-lock time greater than zero to determine a third magnetization value; andcomputing the parameter Rmpfsl includes: computing the first relaxation rate R1ρ(1) based on the first magnetization value and the second magnetization value; andcomputing the second relaxation rate R1ρ(2) based on the first magnetization value and the third magnetization value.

11. The method of claim 10 further comprising: performing one or more additional image acquisition processes to measure an observed longitudinal relaxation rate (R1obs) and an equilibrium magnetization (M0) or the product R1obs·M0,wherein the observed longitudinal relaxation rate R1obs and the equilibrium magnetization M0 or the product R1obs·M0 are used in computing the first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2).

12. The method of claim 1 wherein: each image acquisition process in a first subset of the plurality of image acquisition processes includes applying a spin-lock pulse having the first RF amplitude ω1(1) and the first frequency offset Δω(1); andeach image acquisition process in a second subset of the plurality of image acquisition processes includes applying a spin-lock pulse having the second RF amplitude ω1(2) and the second frequency offset Δω(2).

13. The method of claim 12 wherein: the first subset of the plurality of the image acquisition processes and the second subset of the plurality of image acquisition processes each include at least two image acquisition processes;each image acquisition process in the first subset uses a spin-lock pulse having a time of spin-lock (TSL) that is different from the TSL of each other image acquisition process in the first subset;each image acquisition process in the second subset uses a spin-lock pulse having a TSL that is different from the TSL of each other image acquisition process in the second subset; andthe first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2) are computed from the plurality of images.

14. The method of claim 13 wherein the first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2) are computed from the plurality of images by optimization of the equations:

15. The method of claim 13 wherein performing the plurality of image acquisition processes includes: for each of the plurality of image acquisition processes, using a pulse sequence such that: Mini=M0−M0·e−τ1·R1obs,

16. The method of claim 15 wherein the first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2) are computed from the plurality of images by optimization of the equations:

17. The method of claim 1 wherein each instance of applying an off-resonance spin-lock pulse includes applying an off-resonance spin-lock RF pulse cluster.

18. The method of claim 1 wherein each instance of applying an off-resonance spin-lock pulse includes applying a saturation RF pulse.

19. The method of claim 1 wherein performing each of the plurality of image acquisition processes includes applying at least one preparatory pulse sequence before or after applying the spin-lock pulse and prior to acquiring data.

20. The method of claim 1 wherein the MRI images comprise images of a tissue of a patient.

21. A magnetic resonance imaging (MRI) system comprising: an MRI apparatus having a magnet, a gradient coil, and one or more radiofrequency (RF) coils; anda computer communicably coupled to the MRI apparatus, the computer having a processor, a memory, and a user interface, the processor being configured to: perform a plurality of image acquisition processes using the MRI apparatus to produce a plurality of MRI images, wherein each image acquisition process (i) in the plurality of image acquisition process includes applying an off-resonance spin-lock pulse having an RF amplitude (ω1(i)) and a frequency offset (Δω(i)); andcompute, based on the plurality of MRI images, one or more parameters of magnetization transfer, wherein the one or more parameters of magnetization transfer include a parameter Rmpfsl defined as Rmpfsl=R1ρ(2)−R1ρ(1), wherein: R1ρ(1) is a first relaxation rate in the rotating frame responsive to a spin-lock pulse having a first RF amplitude ω1(1) and a first frequency offset Δω(1);R1ρ(2) is a second relaxation rate in the rotating frame responsive to a spin-lock pulse having a second RF amplitude ω1(2) and a second frequency offset Δω(2); andthe first RF amplitude ω1(1), the first frequency offset Δω(1), the second RF amplitude ω1(2), and the second frequency offset Δω(2) are chosen such that Δω(1)/(ω1(1)=Δω(2)/ω1(2).

22. The system of claim 21 wherein the processor is further configured to compute one or more additional parameters of magnetization transfer based on the parameter Rmpfsl.

23. The system of claim 22 wherein: the one or more additional parameters of magnetization transfer includes a pool size ratio (fb) of a bound pool of protons bound to semi-solid macromolecules that is computed from the parameter Rmpfsl according to the equation

24. The system of claim 23 wherein the saturation rate parameter Rrfc is a function of the RF amplitude (ω1), the off-resonance frequency (Δω), and the transverse relaxation time of the bound pool (T2b).

25. The system of claim 24 wherein the processor is further configured such that the magnetization exchange rate kba and the transverse relaxation time of the bound pool T2b are treated as constants that do not depend on the off-resonance frequency Δω or the RF amplitude ω1.

26. The system of claim 24 wherein the processor is further configured such that the magnetization exchange rate kba and the transverse relaxation time of the bound pool T2b are treated as variables that are determined by fitting to the plurality of MRI images.

27. The system of claim 22 wherein the one or more additional parameters of magnetization transfer include a macromolecular proton fraction (MPF) indicating a fraction of protons that are bound to semi-solid macromolecules.

28. The system of claim 21 wherein the processor is further configured such that performing the plurality of image acquisition processes includes: performing a first image acquisition process using a first spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) without a toggling RF pulse, wherein the first image acquisition process produces a first MRI image (MnoTog(1));performing a second image acquisition process using a second spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) with a toggling RF pulse, wherein the second image acquisition process produces a second MRI image (MTog(1));performing a third image acquisition process using a third spin-lock pulse at the second RF amplitude ω1(2) and the second frequency offset Δω(2) without a toggling RF pulse, wherein the third image acquisition process produces a third MRI image (MnoTog(2)); andperforming a fourth image acquisition process using a fourth spin-lock pulse at the second RF amplitude Ω1(2) and the second frequency offset Δω(2) with a toggling RF pulse, wherein the fourth image acquisition process produces a fourth MRI image (MTog(2)).

29. The system of claim 28 wherein the processor is further configured such that a same time of spin-lock (TSL) is used for each of the first, second, third, and fourth spin-lock pulses and such that the relaxation parameter Rmpfsl is measured according to the equation:

30. The system of claim 21 wherein the processor is further configured such that: performing the plurality of image acquisition processes includes: performing a first image acquisition process using a first spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) with a spin-lock time of zero to determine a first magnetization value;performing a second image acquisition process using a second spin-lock pulse at the first RF amplitude ω1(1) and the first frequency offset Δω(1) with a spin-lock time greater than zero to determine a second magnetization value;performing a third image acquisition process using a third spin-lock pulse at the second RF amplitude ω1(2) and the second frequency offset Δω(2) with a spin-lock time greater than zero to determine a second magnetization value; andcomputing the parameter Rmpfsl includes: computing the first relaxation rate R1ρ(1) based on the first magnetization value and the second magnetization value; andcomputing the second relaxation rate R1ρ(2) based on the first magnetization value and the third magnetization value.

31. The system of claim 30 wherein the processor is further configured to: perform one or more additional image acquisition processes to measure an observed longitudinal relaxation rate (R1obs) and an equilibrium magnetization M0 or the product R1obs·M0,wherein the observed longitudinal relaxation rate R1obs and the equilibrium magnetization M0 or the product R1obs·M0 are used in computing the first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2).

32. The system of claim 21 wherein the processor is further configured such that: each image acquisition process in a first subset of the plurality of image acquisition processes includes applying a spin-lock pulse having the first RF amplitude ω1(1) and the first frequency offset Δω(1); andeach image acquisition process in a second subset of the plurality of image acquisition processes includes applying a spin-lock pulse having the second RF amplitude ω1(2) and the second frequency offset Δω(2).

33. The system of claim 32 wherein the processor is further configured such that: the first subset of the plurality of the image acquisition processes and the second subset of the plurality of image acquisition processes each include at least two image acquisition processes;each image acquisition process in the first subset uses a spin-lock pulse having a time of spin-lock (TSL) that is different from the TSL of each other image acquisition process in the first subset;each image acquisition process in the second subset uses a spin-lock pulse having a TSL that is different from the TSL of each other image acquisition process in the second subset; andthe first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2) are computed from the plurality of images.

34. The system of claim 33 wherein the processor is further configured such that the first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2) are computed from the plurality of images by optimization of the equations:

35. The system of claim 33 wherein the processor is further configured such that performing the plurality of image acquisition processes includes: for each of the plurality of image acquisition processes, using a pulse sequence such that: Mini=M0−M0·e−τ1·R1obs,

36. The system of claim 35 wherein the processor is further configured such that the first relaxation rate R1ρ(1) and the second relaxation rate R1ρ(2) are computed from the plurality of images by optimization of the equations:

37. The system of claim 21 wherein each instance of applying an off-resonance spin-lock pulse includes applying an off-resonance spin-lock RF pulse cluster.

38. The system of claim 21 wherein each instance of applying an off-resonance spin-lock pulse includes applying a saturation RF pulse.

39. The system of claim 21 wherein the processor is further configured such that performing each of the plurality of image acquisition processes includes applying at least one preparatory pulse sequence before or after applying the spin-lock pulse and prior to acquiring data.

40. The system of claim 21 wherein the MRI images comprise images of a tissue of a patient.