Claims
- 1. A system for performing microvolume laser scanning cytometry (MLSC), comprising:(a) one or more sources of excitation light; (b) means for scanning said excitation light over a sample at a plurality of predetermined scan rates; (c) means for collecting and descanning light emitted by said sample in response to said excitation light; (d) a plurality of dichroic filter means for separating said emitted light into a plurality of distinct component wavelengths; (e) a plurality of light detectors responsive to said emitted light, each light detector positioned to collect a distinct one of said component wavelengths, each light detector operatively coupled to an analog electronic filter means having a variable bandwidth; and (f) digital sampling means operatively coupled to each analog electronic filter means and configured to operate at one of a plurality of predetermined digital sampling rates to provide digital signal output from said light detectors; wherein a selected scan rate, a selected analog filter bandwidth, and a selected digital sampling rate are coordinately selected to optimize detection of particles in said sample.
- 2. The system of claim 1, comprising at least four light detectors responsive to said emitted light, wherein said dichroic filter means separate said emitted light into at least four distinct component wavelengths.
- 3. The system of claim 1, wherein said analog electronic filter means comprises switchable filter circuitry.
- 4. The system of claim 1, wherein said selected scan rate, said selected analog filter bandwidth, and said selected digital sampling rate are coordinately selected in dependence on a sample assay.
- 5. The system of claim 1, further comprising a control and data analysis system in communication with said digital sampling means.
- 6. The system of claim 5, wherein said control and data analysis system comprises storage means configured to store a clinical protocol.
- 7. The system of claim 6, wherein said clinical protocol comprises at least one of an optimal scan rate, an optimal analog electronic filter bandwidth, an optimal digital sampling rate, an assay condition of said sample, a medical history related to an organism from which at least part of said sample was obtained, and algorithms for analysis of said digital signal output.
- 8. The system of claim 5, wherein said control and data analysis system is configured to control said scanning means, said analog electronic filter means, and said digital sampling means.
- 9. The system of claim 5, wherein said control and data analysis system is configured to analyze said digital signal output to provide information about said sample.
CROSS REFERENCE TO RELATED APPLICATION
This application claims priority to U.S. Provisional Application No. 60/144,798, filed Jul. 21, 1999, entitled “System for Microvolume Laser Scanning Cytometry.”
US Referenced Citations (27)
Non-Patent Literature Citations (4)
Entry |
Beavis et al. (1996) Cytometry 24:390. |
Dietz et al. (1996) Cytometry 23:177. |
Mujumdar et al. (1993) Bioconjug Chem. 4:105). |
Mahalingam (1993)Cytometry 24:190. |
Provisional Applications (1)
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Number |
Date |
Country |
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60/144798 |
Jul 1999 |
US |