The field of the invention is plant and/or seed system treatment methods. Specifically, electromagnetic plant and/or seed systems and treatment methods that can include modification of any characteristic of a plant and/or seed, or modification of the behavior or survivability of plant and/or seed pests.
By 2050, the human population of the world is expected to increase by more than 35%. It is projected that crop production will need to at least double to feed the world's population in 2050. Currently, chemical or organic fertilizers and pesticides can be used to increase crop production. However, in many cases, fertilizers must be tailored for the specific soil type and climate where the crop is produced and pesticides must be carefully regulated and carefully used. Further, fertilizers and pesticides can have unwanted effects on the environment and the crop production itself. For example, over fertilization can increase growth of unwanted organisms at the treatment area and downstream and can cause depletion of other nutrients in the soil that are not replaced by the fertilizer itself. Resistance to pesticides can occur and pesticides can be detrimental to beneficial organisms in the treatment area or downstream. In addition, some fertilizers, when left on the crop plant can be harmful to human health when contacted or consumed. Also concerning is the likelihood that increasing the world's crop production by the means currently available is not likely to keep up with demand.
Some have attempted to increase crop production or improve desirable characteristics of plants by genetically modifying plants or changing the growth conditions of the plant. Genetic modification can occur through selective breeding, screening plants for desired traits, mutational breeding, gene transfer, gene editing, etc. Some of these modification methods are considered undesirable or unacceptable by some consumers. Further, some of these modification methods are expensive and take many years to develop.
Overall, the need for improvements in this field persists in light of at least the aforementioned drawbacks for the currently available methods and systems.
A solution to at least some of the above-mentioned problems has been discovered. The solution resides in electromagnetic treatments of plants and/or seeds. Disclosed herein are electromagnetic treatment recipes, methods of treatments, and systems and apparatuses to treat plants and/or seeds with said electromagnetic treatments. It has been found that the treatments can modify mass of at least a portion of the plant, yield of the plant, germination rate, germination timing, time to emergence of a coleoptile, time to emergence of a first true leaf, cold tolerance, membrane permeability, nutrient uptake, gene transcription, gene expression, cell growth, cell division, protein synthesis, latent heat flux, carbon assimilation, stomatal conductance, quantum efficiency of PSII reaction centers, efficiency of energy harvesting by oxidized PSII reaction centers, variable fluorescence, fluorescence value at first inflection point, sensible heat flux, net thermal balance, transpiration rate, CO2 assimilation rate, intercellular CO2, stomatal conductance to water vapor, boundary layer conductance to water vapor, total conductance to water vapor, total conductance to CO2, steady-state fluorescence, maximum fluorescence, quantum yield of photosystem II, electron transport rate, quantum yield calculated from CO2 assimilation, non-photochemical quenching, photochemical quenching, non-photochemical quenching, fluorescence, initial fluorescence yield, the chemical profile in at least a portion of the plant, the cannabinoid profile, the terpene profile, trichome content, the time required for harvest readiness, rooting development rate, water use efficiency, nutrient use efficiency, time to develop mature flowers, time to set fruit, plant height, plant width, ratio of vegetative tissue to flower tissue, ratio of vegetative tissue to fruit tissue, quantity of flowering, lateral organs, and/or vegetative node sites, internode spacing, attracting or increasing the amounts of beneficial organisms, and/or repel and/or decrease the amount of pests on the plant and/or seed, as compared to a plant and/or seed that is not treated.
Inducing and/or increasing and/or altering the expression of plant secondary compounds may have many benefits. Plant secondary compounds can include compounds produced as adaptation of plants to their environment and/or compounds not directly involved in normal growth, development, or reproduction of the plant. Plant secondary compounds can include secondary metabolites. Altered, as used herein, may also include a decrease in the expression of a plant secondary compound, or modification of the relative abundance of one or more plant secondary compounds independently or in combination with other plant secondary compounds. Non-limiting examples of the benefits of inducing and/or increasing and/or altering the expression of plant secondary compounds include an impact on plant abiotic stress tolerance, biotic stress tolerance, plant growth and performance, flavor and aroma of plant products, medical, recreational and therapeutic benefits among many more. Of particular interest are terpenes and cannabinoids and the many aforementioned benefits associated with them.
In one aspect, a plant treatment system is disclosed. A “plant” may refer to either the adult plant, seedling, seed, or any portion of a plant and/or seed. The treatment system can be capable of producing any of the electromagnetic plant and/or seed treatments disclosed herein. In some instances, the treatment system includes a function generator configured to provide a voltage and/or current used to generate an electromagnetic field. The function generator may be any component capable of producing a voltage output, and that voltage output, when applied to a radiating structure, generates the electromagnetic field for plant and/or seed treatment. The function generator may generate an arbitrary voltage output to be an electromagnetic signal according to a predetermined or programmable recipe. The recipes may be dynamic and adjust to conditions. For example, if one recipe works best for the first 4 weeks of a plants life during seedling and root development, then a separate recipe works best for the last 4 weeks of a plants life for vegetative development, the system can automatically adjust recipes for the user. In some embodiments, the function generator may generate an electromagnetic signal by modulating a carrier wave. In some embodiments, the function generator may be controlled by a single timer and/or sensor or a combination of timers and/or sensors. In other embodiments, the function generator may be controlled by a computational system configured to receive an input specifying parameters for controlling the function generator and to control the function generator to control generation of the electromagnetic field according to the input. In some instances, the computational system can be merely a system that controls voltage and/or to create a treatment signal. In some instances, the computational system can be a generic computer, a timer, a relay, a function generator, etc. In some embodiments, the function generator may be a transformer. In some instances, the transformer can be an alternating current (AC) to direct current (DC) transformer. In some instances, the transformer can be a DC to AC transformer. In some instances, a transformer is not included and/or not required. In some instances, the treatment system can receive instructions for a treatment recipe wirelessly from a central server. The central server may control any aspect of the electromagnetic plant and/or seed treatment system. The central server may be, for example, a cloud-based management system with AI/machine learning capability or a simple remote control. The treatment system(s) can also receive instructions for more than one treatment recipe. The treatment system(s), in some instances, can change the electromagnetic recipe delivered by the system. In this way, the same system can be used to provide treatment to a plant and/or seed at different stages of growth or development, can be used to treat the same plant and/or seed with different recipes that target a variety of different modifications that target to a variety of different organisms and/or biological process modifications, and/or can be used to treat different plants and/or seeds.
In one aspect, an electromagnetic plant and/or seed treatment is disclosed. The electromagnetic plant and/or seed treatment can include an electromagnetic field comprising a carrier frequency and a carrier waveform. In some instances, a carrier is not used. Optionally, the electromagnetic field can be modulated with a modulating wave to produce a modulated electromagnetic field. In some instances, the electromagnetic field is not modulated. The modulating wave can have a modulating frequency, a modulating waveform, and/or an amplitude modulating index. In some instances, the electromagnetic treatment, at least in part, mimics or enhances naturally occurring changes that can occur in the plant, seed, pest, environment, organisms, or other biological processes. In some instances, the treatment may mimic in part an ion cyclotron resonance frequency of an ion or molecule such as, but not limited to, calcium, potassium, magnesium, iron, copper, phosphate, phosphorous, and/or nitrogen. In some instances, the electromagnetic treatment mimics in part an environmental change, such as, but not limited to a change in ion concentration or electromagnetic field that occurs due to a storm (e.g., increase/decrease in voltage due to the storm).
In another aspect, a method of treating a plant and/or seeds is disclosed. The method can include treating a plant, seed, and/or part of a plant and/or seed, and/or environment surrounding a plant and/or seed, with any one of the electromagnetic plant and/or seed treatments disclosed herein. In some instances, the method is carried out at least in part by any one of the treatment systems disclosed herein. The plant and/or seed treated can be any plant and/or seed, such as a crop plant, an ornamental plant, a medicinal plant, or a plant used for beneficial uses such as ground cover, reduction of soil erosion the receding or changing of shores or banks, providing shade or shelter, reintroduction or increasing the number of plants or plant species in an area, etc. The treatment can be applied, stopped, or modified according to a timing, environmental change, plant life cycle, event such as watering, trigger of a sensor, etc., or can be constant.
In another aspect, a method of treating a plant and/or seeds is disclosed. The method can include treating a plant, seed, and/or part of a plant and/or seed, and/or environment surrounding a plant and/or seed, with any one of the electromagnetic plant and/or seed treatments disclosed herein. In some instances, the method is carried out at least in part by any one of the treatment systems disclosed herein. In some instances, the stress on a plant and/or seeds is increased and/or altered using the electromagnetic systems and treatments disclosed. Increasing stress can increase the synthesis of secondary compounds and expression of specific genes and/or proteins associated with a plant and/or seed stress response, which in some cases may be cannabinoids and/or terpenes. Different applications of the technology may increase and/or alter plant and/or seed stress and/or one or more plant and/or seed processes associated with increase or alteration in plant and/or seed stress. Some non-limiting examples include increased production of terpenes and other secondary compounds, which for example may have commercial or therapeutic value, or be useful for defense against pests; syntheses of heat-shock or other proteins related to abiotic stress; and changes in plant and/or seed form or development. Additionally, stress on a plant and/or seed may be increased and/or altered through one or more secondary effects such as by effecting biotic or abiotic pathogens and/or organisms.
In another aspect, electromagnetic treatment may stimulate or otherwise affect any class of molecules, including but not limited to proteins, carbohydrates, nucleic acids, lipids, and/or any combination thereof, organelles inside, microorganisms inside, outside, associated with, attached to, in proximity to, plants on leaves, fruits, roots, seeds, etc. that affect the growth and vitality of the plant and/or seed.
Disclosed herein is a method of producing secondary compounds in a plant of genus cannabis. In aspects, the methods induce trichome development in a plant of genus cannabis. In aspects, the methods induce and/or alter development of other plant structures. In some embodiments, the secondary compounds are chosen from cannabinoids, terpenes, or flavonoids.
As used herein, the term “terpene” means an organic compound built on an isoprenoid structural scaffold or produced by combining isoprene units. Often, terpene molecules found in plants may produce aroma and/or flavor.
The structure of terpenes are built with isoprene units, which are 5 carbon structures. Flavonoids are generally considered to be 15 carbon structures with two phenyl rings and a heterocyclic ring. So, there could be an overlap in which a flavonoid could be considered a terpene. However, not all terpenes could be considered flavonoids. The systems and methods herein include any of cannabinoids, terpenes, or flavonoids, in addition to other plant compounds.
The term terpene includes but is not limited to Hemiterpenes, Monoterpenols, Terpene esters, Diterpenes, Monoterpenes, Polyterpenes, Tetraterpenes, Terpenoid oxides, Sesterterpenes, Sesquiterpenes, Norisoprenoids, or their derivatives.
Derivatives of terpenes include but are not limited to terpenoids in their forms of hemiterpenoids, monoterpenoids, sesquiterpenoids, sesterterpenoid, sesquarterpenoids, tetraterpenoids, Triterpenoids, tetraterpenoids, Polyterpenoids, isoprenoids, and steroids. They may be forms: α-, β-, γ-, oχo-, isomers, or combinations thereof.
Examples of terpenes include but are not limited to: 7,8-dihydroionone, Acetanisole, Acetic Acid, Acetyl Cedrene, Anethole, Anisole, Benzaldehyde, Bergamotene (a-cis-Bergamotene) (a-trans-Bergamotene), Bisabolol (β-Bisabolol), Borneol, Bornyl Acetate, Butanoic/Butyric Acid, Cadinene (a-Cadinene) (γ-Cadinene), Cafestol, Caffeic acid, Camphene, Camphor, Capsaicin, Carene (Δ-3-Carene), Carotene, Carvacrol, Carvone, Dextro-Carvone, Laevo-Carvone, Caryophyllene (β-Caryophyllene), Caryophyllene oxide, Castoreum Absolute, Cedrene (a-Cedrene) (β-Cedrene), Cedrene Epoxide (a-Cedrene Epoxide), Cedrol, Cembrene, Chlorogenic Acid, Cinnamaldehyde (a-amyl-Cinnamaldehyde) (a-hexyl-Cinnamaldehyde), Cinnamic Acid, Cinnamyl Alcohol, Citronellal, Citronellol, Cryptone, Curcumene (a-Curcumene) (γ-Curcumene), Decanal, Dehydrovomifoliol, Diallyl Disulfide, Dihydroactinidiolide, Dimethyl Disulfide, Eicosane/lcosane, Elemene (β-Elemene), Estragole, Ethyl acetate, Ethyl Cinnamate, Ethyl maltol, Eucalyptol/1,8-Cineole, Eudesmol (a-Eudesmol) (β-Eudesmol) (γ-Eudesmol), Eugenol, Euphol, Farnesene, Farnesol, Fenchol (s-Fenchol), Fenchone, Geraniol, Geranyl acetate, Germacrenes, Germacrene B, Guaia-1 (10), 1 1-diene, Guaiacol, Guaiene (a-Guaiene), Gurjunene (a-Gurjunene), Herniarin, Hexanaldehyde, Hexanoic Acid, Humulene (a-Humulene) (β-Humulene), lonol (3-oxo-a-ionol) (β-lonol), lonone (a-lonone) (β-lonone), Ipsdienol, Isoamyl acetate, Isoamyl Alcohol, Isoamyl Formate, Isoborneol, Isomyrcenol, Isopulegol, Isovaleric Acid, Isoprene, Kahweol, Lavandulol, Limonene, γ-Linolenic Acid, Linalool, Longifolene, a-Longipinene, Lycopene, Menthol, Methyl butyrate, 3-Mercapto-2-Methylpentanal, Mercaptan/Thiols, β-Mercaptoethanol, Mercaptoacetic Acid, Allyl Mercaptan, Benzyl Mercaptan, Butyl Mercaptan, Ethyl Mercaptan, Methyl Mercaptan, Furfuryl Mercaptan, Ethylene Mercaptan, Propyl Mercaptan, Thenyl Mercaptan, Methyl Salicylate, Methylbutenol, Methyl-2-Methylvalerate, Methyl Thiobutyrate, Myrcene (β-Myrcene), γ-Muurolene, Nepetalactone, Nerol, Nerolidol, Neryl acetate, Nonanaldehyde, Nonanoic Acid, Ocimene, Octanal, Octanoic Acid, P-cymene, Pentyl butyrate, Phellandrene, Phenylacetaldehyde, Phenylethanethiol, Phenylacetic Acid, Phytol, Pinene, s-Pinene, Propanethiol, Pristimerin, Pulegone, Quercetin, Retinol, Rutin, Sabinene, Sabinene Hydrate, cis-Sabinene Hydrate, trans-Sabinene Hydrate, Safranal, a-Selinene, a-Sinensal, β-Sinensal, β-Sitosterol, Squalene, Taxadiene, Terpin hydrate, Terpineol, Terpine-4-ol, a-Terpinene, Y-Terpinene, Terpinolene, Thiophenol, Thujone, Thymol, a-Tocopherol, Tonka Undecanone, Undecanal, Valeraldehyde/Pentanal, Verdoxan, a-Ylangene, Umbelliferone, or Vanillin.
The term “cannabinoid” means any substance that acts upon a cannabinoid receptor. For example the term cannabinoid includes cannabinoid ligands such as agonists, partial agonists, inverse agonists, or antagonists, as demonstrated by binding studies and functional assays. In many examples, a cannabinoid can be identified because its chemical name will include the text string “*cannabi* in the name. Within the context of this application, where reference is made to a particular cannabinoid, each of the acid and/or decarboxylated forms are contemplated as both single molecules and mixtures.
Examples of cannabinoids include but are not limited to compounds belonging to any of the following classes of molecules, their derivatives, salts, or analogs: Tetrahydrocannabinol (THC), Tetrahydrocannabivarin (THCV), Cannabichromene (CBC), Cannabichromanon (CBCN), Cannabidiol (CBD), Cannabielsoin (CBE), Cannabidivarin (CBDV), Cannbifuran (CBF), Cannabigerol (CBG), Cannabicyclol (CBL), Cannabinol (CBN), Cannabinodiol (CBND), Cannabitriol (CBT), Cannabivarin (CBV), and Isocanabinoids.
Some of the impacts caused by the electromagnetic treatments described herein include but are not limited to:
Increased and/or altered presence of one or more above ground biomass plant structures.
Increased and/or altered presence of one or more below ground biomass plant structures.
Increased and/or altered presence of one or more root development plant structures.
Increased and/or altered presence of one or more adventitious root development plant structures.
Increased and/or altered presence of one or more cannabinoids.
Increased and/or altered presence of one or more terpenes.
Increased and/or altered presence of one or more intra cellular ions.
Increased and/or altered presence of one or more extra cellular ions.
Increased and/or altered presence of one or more primary compounds.
Increased and/or altered presence of one or more secondary metabolites.
Increased and/or altered presence of one or more secondary compounds.
Increased and/or altered presence of one or more secondary metabolites.
Increased and/or altered presence of one or more ions.
Increased and/or altered overall cannabinoids.
Increased and/or altered overall terpenes.
Increased and/or altered overall intra cellular ions.
Increased and/or altered overall extra cellular ions.
Increased and/or altered overall secondary compounds.
Increased and/or altered overall secondary metabolites.
Increased and/or altered overall ions.
Increased and/or altered abiotic tolerance.
Increased and/or altered biotic tolerance.
Increased and/or altered flavor.
Increased and/or altered aroma.
Increased and/or altered therapeutic value.
Increased and/or altered resistance to pests.
Increased and/or altered resistance to viruses.
Increased and/or altered resistance to insects.
Increased and/or altered resistance to microbes.
Increased and/or altered resistance to fungi.
Increased and/or altered resistance to biotic stress.
Increased and/or altered resistance to abiotic stress.
Increased and/or altered resistance to moisture stress.
Increased and/or altered resistance to nutrient stress.
Increased and/or altered resistance to temperature stress.
Increased and/or altered resistance to atmospheric carbon concentration stress.
Increased and/or altered resistance to light stress.
Increased and/or altered respiration.
Increased and/or altered cell wall plasticity.
Increased and/or altered cell wall permeability.
Increased and/or altered membrane plasticity.
Increased and/or altered membrane permeability.
Increased and/or altered organelle plasticity.
Increased and/or altered organelle permeability.
Increased and/or altered changes in internal ionic concentration.
Increased and/or altered changes in external ionic concentration.
Increased and/or altered changes in internal molecular concentration.
Increased and/or altered changes in external molecular concentration.
Increased and/or altered changes in molecular orientation.
Increased and/or altered activation energy required to move ions across a membrane.
Increased and/or altered activation energy required to move ions across a cell wall.
Increased and/or altered internal microbial communities.
Increased and/or altered external microbial communities.
Increased and/or altered plant and/or seed physiology.
Increased and/or altered osmoregulation.
Decreased and/or altered time to emergence of a coleoptile.
Decreased and/or altered time to emergence of a first true leaf.
Increased and/or altered cold tolerance.
Increased and/or altered drought tolerance
Increased and/or altered assimilation.
Increased and/or altered transpiration.
Increased and/or altered water acquisition.
Increased and/or altered water use efficiency.
Increased and/or altered water potential.
Increased and/or altered nutrient acquisition.
Increased and/or altered nutrient use efficiency.
Increased and/or altered nutrient use productivity.
Increased and/or altered plant maturation.
Increased and/or altered vigor.
Increased and/or altered time elapsed between life cycle development points.
Increased and/or altered germination.
Increased and/or altered metabolism.
Increased and/or altered carbon assimilation.
Increased and/or altered sequestration.
Increased and/or altered plant structural morphology.
Increased and/or altered organelle activity.
Increased and/or altered protein activity.
Increased and/or altered carbohydrate activity.
Increased and/or altered nucleic acid activity.
Increased and/or altered lipid activity.
Increased and/or altered microorganism activity.
Increased and/or altered transcription.
Increased and/or altered gene expression.
Increased and/or altered cell growth.
Increased and/or altered cell division.
Increased and/or altered protein synthesis.
Increased and/or altered latent heat flux.
Increased and/or altered carbon assimilation.
Increased and/or altered stomatal conductance.
Increased and/or altered quantum efficiency of PSII reaction centers.
Increased and/or altered efficiency of energy harvesting by oxidized PSII reaction centers.
Increased and/or altered variable fluorescence.
Increased and/or altered fluorescence value at first inflection point.
Increased and/or altered sensible heat flux.
Increased and/or altered net thermal balance.
Increased and/or altered intercellular CO2.
Increased and/or altered stomatal conductance to water vapor.
Increased and/or altered boundary layer conductance to water vapor.
Increased and/or altered total conductance to water vapor.
Increased and/or altered total conductance to CO2.
Increased and/or altered steady-state fluorescence.
Increased and/or altered maximum fluorescence.
Increased and/or altered quantum yield of photosystem II.
Increased and/or altered electron transport.
Increased and/or altered quantum yield calculated from CO2 assimilation.
Increased and/or altered non-photochemical quenching.
Increased and/or altered photochemical quenching.
Increased and/or altered non-photochemical quenching.
Increased and/or altered fluorescence.
Increased and/or altered initial fluorescence yield.
Increased and/or altered the chemical profile in at least a portion of the plant and/or seed.
Increased and/or altered the cannabinoid profile.
Increased and/or altered the terpene profile.
Increased and/or altered the flavonoid profile.
Increased and/or altered the time required for harvest readiness.
Increased and/or altered rooting development rate.
Increased and/or altered water use efficiency.
Increased and/or altered nutrient use efficiency.
Increased and/or altered time to develop mature flowers.
Increased and/or altered time to set fruit.
Increased and/or altered plant height.
Increased and/or altered plant width.
Increased and/or altered ratio of vegetative tissue to flower tissue.
Increased and/or altered ratio of vegetative tissue to fruit tissue.
Increased and/or altered quantity of flowering.
Increased and/or altered lateral organs.
Increased and/or altered and/or vegetative node sites.
Increased and/or altered internode spacing.
Increased and/or altered attracting or increasing the amounts of beneficial organisms.
Increased and/or altered repelling of the amount of pests on the plant and/or seed.
Increased and/or altered therapeutic value of plant and/or seed-derived products such as, but not limited to, anticancer and chemotherapy, antimicrobial, antifungal, antiviral, antiparasitic, anti-inflammatory, and/or antihyperglycemic anti-inflammatory products.
Any of the above impacts may be observed or induced alone or in any combination thereof.
The following includes definitions of various terms and phrases used throughout this specification.
The terms “about,” “approximately,” and “substantially” are defined as being close to, as understood by one of ordinary skill in the art. In one non-limiting instance, the terms are defined to be within 10%, preferably within 5%, more preferably within 1%, and most preferably within 0.5%.
The terms “wt. %,” “vol. %,” or “mol. %” refers to a weight, volume, or molar percentage of a component, respectively, based on the total weight, the total volume, or the total moles of material that includes the component. In a non-limiting example, 10 grams of a component in 100 grams of the material that includes the component is 10 wt. % of component.
The use of the words “a” or “an” when used in conjunction with any of the terms “comprising,” “including,” “containing,” or “having” in the claims or the specification may mean “one,” but it is also consistent with the meaning of “one or more,” “at least one,” and “one or more than one.”
The words “comprising” (and any form of comprising, such as “comprise” and “comprises”), “having” (and any form of having, such as “have” and “has”), “including” (and any form of including, such as “includes” and “include”), or “containing” (and any form of containing, such as “contains” and “contain”) are inclusive or open-ended and do not exclude additional, unrecited elements or method steps.
The compositions and process of the present invention can “comprise,” “consist essentially of,” or “consist of” particular ingredients, components, compositions, etc., disclosed throughout the specification. With respect to the transitional phase “consisting essentially of,” in one non-limiting aspect, a basic and novel characteristic of the treatment systems and plant and/or seed treatments disclosed herein is that the treatments modify plants and/or seeds through contacting the plant and/or seed with an electromagnetic field designed to modify the plant and/or seed and the systems herein are capable of producing said treatments. In some instances, the systems are capable of receiving instructions for treatments and producing said treatments.
Other objects, features and advantages of the present invention will become apparent from the following figures, detailed description, and examples. It should be understood, however, that the figures, detailed description, and examples, while indicating specific embodiments of the invention, are given by way of illustration only and are not meant to be limiting. Additionally, it is contemplated that changes, combinations, and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.
Advantages of the present invention may become apparent to those skilled in the art with the benefit of the following non-limiting detailed description and upon reference to the accompanying non-limiting drawings. The drawings may not be to scale.
    
    
    
    
    
    
    
    
    
    
Electromagnetic treatment recipes, methods of treatments, and systems and apparatuses to treat plants and/or seeds with said electromagnetic treatments disclosed herein have been developed to increase and/or alter the weight of at least a portion of the plant, yield of the plant, germination rate, germination timing, time to emergence of a coleoptile, time to emergence of a first true leaf, cold tolerance, membrane permeability, nutrient uptake, gene transcription, gene expression, cell growth, cell division, protein synthesis, latent heat flux, carbon assimilation, stomatal conductance, the chemical profile in at least a portion of the plant and/or seed, the cannabinoid profile, the terpene profile, trichome content, the time required for harvest readiness, quantity of flowering sites, internode spacing, plant morphology, and/or repel and/or decrease the amount of pests on the plant and/or seed, as compared to a plant and/or seed that is not treated. In some instances, the electromagnetic treatment, at least in part, mimics or enhances naturally occurring changes that can occur in the plant, seed, pest, or environment. As a non-limiting example, the treatment may mimic in part an ion cyclotron resonance frequency of an ion such calcium, potassium, magnesium, iron, copper, hydronium, phosphate, phosphorous, and/or nitrogen. As another non-limiting example, the electromagnetic treatment mimics in part an environmental change, such as, but not limited to a change in ion concentration or electromagnetic field that occurs due to a storm (e.g., increase electric field in voltage per meter due to the storm).
U.S. Provisional Patent Application No. 62/884,778, filed Aug. 9, 2019, is hereby referenced and incorporated in its entirety
  
The system 100 also includes a computational system 114 configured to receive an input specifying the electromagnetic field and optionally the modulating wave and control the function generator 116 to control the generation of the electromagnetic field 122. The function generator 116 may be, for example, a software defined radio (SDR), a transformer, or another waveform generation circuit. The input to the function generator 116 may be a decoded electromagnetic treatment recipe that specifies parameters such as voltage, amplitude, carrier frequency, modulation pattern, etc. The function generator 116 may produce the electromagnetic signal 118 by generating a carrier wave in accordance with the recipe and then optionally modulating the carrier wave in accordance with the recipe.
The electromagnetic treatment recipe may be stored in memory 112, where the recipe is read out by the computational system 114 and decoded. In some embodiments, the treatment system can receive instructions for a treatment recipe wirelessly. In some embodiments, the treatment system stores a recipe book, and individual recipes within the book are unlocked by wireless communications or entering codes into the user system 110. The treatments system 100 can also receive instructions for more than one treatment recipe. The treatment system 100, in some instances, can change the electromagnetic recipe. In this way, the same system can be used to provide treatment to a plant and/or seed at different stages of growth or development, can be used to treat the same plant and/or seed with different recipes that target a variety of different plant/seed/pest modifications, and/or can be used to treat different plants and/or seeds. In some embodiments, the user system 110 may also include a communications adapter, such as a wireless communications adapter, to perform functions described in more detail below.
Other examples of electromagnetic treatment recipe delivery systems are shown in 
  
1. Radiating Structures
Radiating structure 120 shown in 
Other example radiating structures are shown in 
  
In some embodiments, the radiating structure may be arranged in a grid configuration, a mesh configuration, and/or may be a plate. 
The radiating structure, such as wire, grid, mesh, and/or plate may be folded, bent, formed into shapes such as tubes, coils, cubes, rectangular tubes, cones, pyramids, spheres, etc., may be in multiple pieces, may be made with large openings or small openings formed by the radiating structures, such as wires, grid, mesh, and/or plate(s). In some instances, radiating structure may divide plants and/or seeds. In some instances, the radiating structure may surround the plants and/or seeds. A plurality of radiating structures can be arranged in parallel, perpendicular, or any other arrangement in relation to each other.
  
In 
In some instances, the radiating structure, the whole system, or a portion of the system is movable during delivery of the electromagnetic treatment. As non-limiting examples, the system or the radiating structures can be mounted on a wheeled trailer and/or cart, on a vehicle such as a tractor, on a track and/or pulley system, on an elevator, etc.
2. Recipe Delivery
Referring back to the electromagnetic treatment recipe delivery system 100 of 
In some embodiments, the recipes may thus be maintained at a central facility from which the recipes or authorization codes to unlock certain recipes from a recipe book are provided to users of electromagnetic treatment recipe delivery systems. 
The server 410 may distribute information, including recipes or authorization codes, to the user systems 110 through a variety of techniques. In one example, the server 410 may connect to the user systems 110 through a public network 420, such as the Internet, through wired or wireless communications. In another example, the server 410 may connect to the user systems 110 through a proprietary radio transmission tower 430. In a further example, the server 410 may connect to the user systems 110 through satellite relay 440. In another example, the server 410 may connect to the user systems 110 through removable media, such as a USB data storage dongle 440. A user may use another computing device to obtain a secured recipe, code, key, or certificate that is loaded on the dongle 440 and coupled to the user system 110 for read-out.
One example transaction for unlocking recipes in the electromagnetic treatment recipe delivery systems is shown in 
At step 504, the server 410 transmits the encrypted recipe or authorization code corresponding to the purchased recipe to a user device for loading to a computational system or directly to a computational system, such as the computational control system 114 of user system 110. An encrypted recipe 602 may be transmitted by the server 410 over the public network 420 to a user system 110. Example embodiments for delivery of the recipe may include transmission over the air (OTA), delivery of a recipe to a user or technician to manual enter the recipe in the plant and/or seed treatment system, and/or delivery of a file to be loaded into the plant and/or seed treatment system. The recipes can be monetized through business models such as software as a service (SaaS), a subscription model wherein the farm subscribes to a specific recipe and pays a monthly fee for use based on what they are growing and how much area they want treated, a lease model, and/or a direct sale model.
At block 506, the user system 110 decodes the recipe or code and configures the function generator 116 in accordance with the purchased recipe. Steps performed in the transmission of the recipe or code and performed in the storing and processing of data within the user system 110 may be performed in a manner to maintain security of the purchased recipe. For example, the recipe when stored in the memory 112 may be stored as encrypted data that cannot be read as plain text. The computational control system 114 of the user system 110 may be an encrypted digital signal processor (DSP) with a trusted platform module (TPM) that may assist in the reading and securing of the recipes. When desired or scheduled, the encrypted DSP decodes the recipe and provides control signals to the functional generator 116 to produce an electromagnetic signal. In some embodiments, the computational control system 114 may provide secure systems for logging number of uses of each recipe and transmitting the logged data back to the server 410. In one example, a schedule for the application of electromagnetic fields may be included in the recipe, where the exposure to electromagnetic fields is not intended to be continuous. In another example, a recipe may specify exposure to electromagnetic fields of different characteristics during different period of time during a day, a week, a month, or a year.
3. Multiple Radiating Structure Configurations
For large installations of plant nurseries that exceed the power output capability of a single user system 110, power amplifiers may be distributed throughout a location to power separate radiating structures within the location. For example, one function generator may be coupled to multiple power amplifiers and transformers throughout the location. As another example, a location may have four different grow rooms in one facility, wherein the plant and/or seed treatment system includes one computer, four amplifiers, and 64 transformers in one arrangement, or four computers, 64 amplifiers, and 64 transformers in another arrangement, or four computers, four amplifiers, and 16 transformers in yet another arrangement.
Another example is a farm with 4 different grow rooms in one facility. We might have 1 computer, 4 amplifiers, and 64 transformers in such an arrangement. Or we could have 4 computers, 64 amplifiers and 64 transformers. Another arrangement might include 4 computers, 4 amplifiers, and 16 transformers. Finally, multiple radiating structures may apply the same and/or different recipes to the same plant(s) and/or seed(s) depending on the desired outcomes.
One example setup is shown in 
Some or all of the electromagnetic plant and/or seed treatments described herein can be produced by any of the treatment systems described herein. The plant and/or seed treatments can include an electromagnetic field comprising a carrier frequency and a carrier waveform. In some instances, a carrier is not used. The electromagnetic field can be modulated with a modulating wave to produce a modulated electromagnetic field. In some instances, the electromagnetic fields are not modulated. The modulating wave can have a modulating frequency, a modulating waveform, and/or an amplitude modulating index. Not to be bound by theory, but it is believed that modifying one or more of the parameters disclosed herein may help increase the response of a plant's and/or seed's cellular processes to the electromagnetic treatment. Any of the electromagnetic parameters used herein may be positive or negative, and it is understood that electromagnetic parameters provided as a positive or negative number equally include both the positive and negative applications of that parameter.
1. Carrier Waveform:
Any carrier waveform can be used when a carrier wave is used. Carrier waveforms that can be used include, but are not limited to, static, pulsed, square, sine, triangular, sawtooth, damped pulse, rectangular, ramped, cardiogram, or amplitude varying.
2. Carrier Frequency:
The carrier frequency of the treatment can be any frequency from 0 Hz to 6 Ghz. The carrier frequency can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 Hz, or any range thereof or frequency there between. The carrier frequency can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 KHz, or any range thereof or frequency there between. The carrier frequency can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 MHz, or any range thereof or frequency there between. The carrier frequency can be 1, 2, 3, 4, 5, or 6 GHz, or any range thereof or frequency there between. The carrier frequency can be any range of the frequencies in this paragraph or frequency there between. In some instances, the carrier frequency is 0 Hz to 5.875 GHz, 0 to 200 Hz, 1 to 17 MHz, 1.4 to 15.1 MHz, 40 to 55 MHz, 45 to 50 MHz, 48 to 49 MHz, or 48.468 MHz. In some instances, the carrier frequency is a frequency with the Industrial, Scientific, and Medical (ISM) frequency bands. The ISM frequency bands can be frequencies designated as defined by the ITU Radio Regulations. The ISM frequency bands can include frequencies set aside for uses other than for telecommunications, though some of these frequencies have be used for telecommunications.
Not to be bound by theory, but it is believed that use of a carrier frequency that is dampened by tissue of the plant and/or seeds treated may help provide benefits to the plant and/or seeds. In some instances, the carrier frequency used is the frequency that is most dampened by plant and/or seed tissue. Additionally or alternatively, the electromagnetic treatments may stimulate or otherwise affect any class of molecules, including but not limited to proteins, carbohydrates, nucleic acids, lipids, and combination thereof, organelles inside, microorganisms inside, outside, associated with, attached to, in proximity to, plants on leaves, fruits, roots, seeds, etc. that affect the growth and vitality of the plant and/or seed.
3. Modulation Wave and Waveform:
The modulating wave, when used, can modulate the carrier wave's frequency and/or amplitude. In some instances, the modulating wave modulates the carrier's frequency. In some instances, the modulation wave modulates the carrier's amplitude. In some instances, the modulation wave modulates the carrier's frequency and amplitude. In other instances, the modulation may include amplitude modulation, frequency modulation, phase modulation, amplitude-shift keying, frequency-shift keying, phase-shift keying, and/or pulse width modulation.
Any modulation waveform can be used when a modulation wave is used. Modulation waveforms that can be used include, but are not limited to pulsed, square, sine, triangular, sawtooth, static, damped pulse, rectangular, ramped, cardiogram, or amplitude varying. In some instances, the modulation waveform is square.
4. Modulation Frequency:
The modulation wave frequency of the treatment can be any frequency. In some instances, the modulation frequency is from 0 Hz to 6 GHz. The modulation frequency can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 Hz, or any range thereof or frequency there between. The modulation frequency can be <1, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 KHz, or any range thereof or frequency there between. The modulation frequency can be <1, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 MHz, or any range thereof or frequency there between. The modulation frequency can be <1, 1, 2, 3, 4, 5, or 6 GHz, or any range thereof or frequency there between. The modulation frequency can be any range of the frequencies in this paragraph or frequency there between. In some instances, the modulation frequency is 0 to 200 Hz. In some instances, the modulation frequency is 188, 60, 50, 16, or 0 Hz. In some instances, the modulation frequency is 50 Hz.
5. Amplitude Modulation:
The amplitude of a carrier wave can be modified to provide an amplitude modulated wave. The amplitude of the carrier wave can be modified from 0% to 120%. The amplitude can be modified <1, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120%, or any range thereof or amplitude there between. In some instances, the modulation is in a square, sine, or sawtooth waveform pattern. In some instances, the amplitude is not modified. In some instances, the amplitude is modified from 5% to 50%. In some instances, the amplitude is modified 30%.
6. Magnetic Field Strength
The magnetic field strength of the treatment can be any magnetic field strength. In some instances, the magnetic field strength is from 0.001 micro Tesla to 100 mega Tesla or greater. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 nano Tesla, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190,200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 micro Tesla, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 milli Tesla, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 Tesla, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 kilo Tesla, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 mega Tesla, or any range thereof or strength there between. The magnetic field strength can be any range of the field strengths in this paragraph or field strengths there between. In some instances, the magnetic field strength is 0 to 250 micro Tesla. In some instances, the magnetic field strength is 1 to 9 milli Tesla.
7. Electric Field Strength
The electric field strength of the treatment can be any electric field strength. In some instances, the electric field strength is from 0.001 micro volts per meter to 100 mega volts per meter or greater. In some instances, the electric field strength is of positive polarity. In some instances, the electric field strength is of negative polarity. In some instances, the electric field strength switches between positive and negative polarity. The following strength examples shown are absolute values and each represent positive polarity or negative polarity electric field strengths. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340,350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 micro volts per meter, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 milli volts per meter, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 volts per meter, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 kilo volts per meter, or any range thereof or strength there between. The field strength can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 mega volts per meter, or any range thereof or strength there between. The electric field strength can be any range of the field strengths in this paragraph or field strengths there between. In some instances, the electric field strength is 0 to 1.5 volts per meter. In some instances, the electric field strength is 0 to 50 volts per meter. In some instances, the electric field strength is 0 to 5 kilo volts per meter. In some instances, the electric field strength is 10 to 15 kilo volts per meter. In some instances, the electric field strength is 200 to 300 kilo volts per meter.
8. Voltage
The voltage applied to the plant and/or seed can be any voltage. In some instances, the voltage is from 0.001 micro Volts to 100 mega Volts or greater. In some instances, the voltage is of positive polarity. In some instances, the voltage is of negative polarity. In some instances, the voltage switches between positive and negative polarity. The following strength examples shown are absolute values and represent example positive polarity and negative polarity voltages. The voltage can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320,330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 micro Volts, or any range thereof or voltage there between. The voltage can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 milli Volts, or any range thereof or voltage there between. The voltage can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 volts, or any range thereof or voltage there between. The voltage can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390,400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 kilo volts, or any range thereof or voltage there between. The voltage can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 mega volts, or any range thereof or voltage there between. The voltage can be any range of the field strengths in this paragraph or field strengths there between. In some instances, the voltage is 0 to 50 volts. In some instances, the voltage is 0 to 5 kilo volts. In some instances, the voltage is 10 to 15 kilo volts. In some instances, the voltage is 17 to 33 kilo volts.
9. Duration
The duration in which treatment is applied to the plant can be a variety of durations. In some instances, the duration is from 0.001 micro seconds to 3 months or greater. In some instances, the treatment is continuously applied. In some instances, the treatment is subcontinuously, periodically, or temporarily applied. In some instances, the treatment is routinely applied according to a timed schedule or algorithm. In some instances, the treatment is applied based on an external factor such as irrigation schedule, light intensity, or planting schedule. The following durations shown represent example durations. The duration can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 micro seconds, or any range thereof or time there between. The duration can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320,330, 340, 350, 360,370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 milliseconds, or any range thereof or time there between. The duration can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340,350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 seconds, or any range thereof or seconds there between. The duration can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340,350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 minutes, or any range thereof or minutes there between. The duration can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 hours, or any range thereof or hours there between. The duration can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320, 330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460, 470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600, 610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880, 890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990 days, or any range thereof or days there between. The duration can be any range of the durations in this paragraph or durations there between. In some instances, the duration is 10 milliseconds. In some instances, the duration is 60 minutes. In some instances, the duration is 3 hours. In some instances, the duration is 56 days.
The electromagnetic treatment recipes, methods of treatments, and systems and apparatuses to treat plants and/or seeds with said electromagnetic treatments disclosed herein have been found to increase and/or decrease the weight of at least a portion of the plant, yield of the plant, germination rate, germination timing, time to emergence of a coleoptile, time to emergence of a first true leaf, cold tolerance, membrane permeability, nutrient uptake, gene transcription, gene expression, cell growth, cell division, protein synthesis, latent heat flux, carbon assimilation, stomatal conductance, the chemical profile in at least a portion of the plant, the cannabinoid profile, the terpene profile, trichome content, the time required for harvest readiness, quantity of flowering sites, internode spacing, and/or repel and/or decrease the amount of pests on the plant and/or seed, as compared to a plant and/or seed that is not treated.
1. Plants
The plant treated can be any plant and/or seed, such as a crop plant, an ornamental plant, a medicinal plant, lumber trees, or a plant used for beneficial uses such as ground cover, reduction of soil erosion the receding or changing of shores or banks, providing shade or shelter, reintroduction or increasing the number of plants or plant species in an area, etc.
In some embodiments, the plant is selected from any plant of the kingdom Plantae. In embodiments, the plant belongs to the subkingdom Viridiplantae. In embodiments, the plant belongs to the infrakingdom Streptophta. In embodiments, the plant belongs to the superdivision Embryophyta. In embodiments, the plant belongs to the division Tracheophyta. In embodiments, the plant belongs to the subdivision Spermatophytina. In embodiments, the plant belongs to the class Magnoliopsida. In embodiments, the plant belongs to a superorder selected from Rosanae and Asteranae. In embodiments, the plant belongs to an order selected from Rosales, Brassicales Asterales, Vitales, and Solanales. In embodiments, the plant belongs to a family selected from Brassicaceae, Asteracae, Aracea, Vitacaea, Solanacaea, and Cannabaceae. In embodiments, the plant belongs to a genus selected from Humulus, Brassica, Eruca, Lactuca, Vitis, Solanum and Cannabis. In embodiments, the plant is selected from the species Humulus japonicus, Humulus lupulus, Lemna minor, Brassica rapa, Eruca vesicaria, Lactuca biennis, Lactuca canadensis, Lactuca floridana, Lactuca graminifolia, Lactuca hirsute, Lactuca indica, Lactuca ludoviciana, Lactuca X morssii, Lactuca sagilina, Lactuca sativa, Lactuca serriola, Lactuca terrae-novae, Lactuca virosa, Vitis acerifolia, Vitis aestivalis, Vitis amurensis, Vitis arizonica, Vitis X bourquina, Vitis californica, Vitis X champinii, Vitis cinerea, Vitis coriacea, Vitis X doaniana, Vitis girdiana, Vitis labrusca, Vitis X labruscana, Vitis monticola, Vitis mustangensis, Vitis X novae-angliae, Vitis palmata, Vitis riparia, Vitis rotundifolia, Vitis rupestris, Vitis shuttleworthii, Vitis tillifolia, Vitis vinifera, Vitis vulpina, Cannabis sativa, and Solanum lycopersicum. In embodiments, the plant is a cultivar or subspecies of any of the above referenced species. In embodiments, the plant is selected from any of the plants commonly referred to as lettuce, arugula, bok choy, tomato, cannabis, hemp, grape, hops, spinach, sunflower, canola, flax corn, rice, wheat, oat, barley, soybean, bean, pea, legume, chickpea, sorghum, sugar cane, sugar beet, cotton, potato, turnip, carrot, onion, cantaloupe, watermelon, blueberry, cherry, apple, pear, peach, cacti, date, fig, coconut, almond, walnut, pecan, cilantro, broccoli, cauliflower, zucchini, squash, pumpkin, duckweed, and mizuna, and any cultivars or subspecies thereof.
Any of the effects disclosed herein may apply to one or more plants and/or seeds. Specific, non-limiting examples of plants that aspects of the invention may apply to include hemp, cannabis, Cannabis sativa, Cannabis indica, Cannabis ruderalis, Zea mays, and Glycine max, or any related cultivars or subspecies thereof.
2. Pests
The electromagnetic treatment recipes, methods of treatments, and systems and apparatuses disclosed herein in some instances, can deter pests, repel pests, modify the behavior of pests, and/or even kill and/or decrease the fertility of pests. In some instances, the treatment may modify a plant and/or seed so that the plant and/or seed itself can deter pests, repel pests, modify the behavior of pests, and/or even kill and/or decrease the fertility of pests.
In some embodiments, the pest can include, but is not limited to, invertebrate pests such as insects, arthropods, mites, and nematodes, fungi, bacteria, animals, or disease causing organisms. Non-limiting examples of pest can include, but are not limited to Achatina fulica, Adelges tsugae, Agrilus planipennis, Ampullaria gigas, Bruchus rupfmanus, Callosobruchus maculatus, Cinara cupressi, Dendroctonus valensi, Eriosona lanigerum, Euglandina rosea, Hemiberlesia pilysophila, Hyphantria cunea, Incisitermes minor, Lehmannia valentiana, Linepithema humile, Liriomyza sativae, Nylanderia fulva, Opogona sacchari, Oracella acuta, Pheidole megacephala, Pomacea canaliculataI, Schislocerca americanaI, Sirex nocilloI, Solenopsis invicta, Solenopsis mandibularis, Trogoderma granariumI, Vespula vulgaris, Viteus vitifoliae, Wasmannia auropunctataI, Zabrotes subfasciatus, Callosobruchus Chinensis, Sitophilus zeamais, Tribolium castaneum, Epilachna vigintioctomaculata, Agriotes fuscicollis, Anomala rufocuprea, Leptinotarsa decemhneata, Diabrotica spp., Monochamus alternatus, Lissorhoptrus oryzophilus, Lymantria dispar, Malacosoma neustria, Pieris rapae, Spodoptera litura, Mamestra brassicae, Chilo suppressalis, Pyrausta nubilalis, Ephestia cautella, Adoxophyes orana, Carpocapsa pomonella, Galleria mellonella, Plutella maculipennis, Heliothis Phyllocnistis citrella, Nephotettix cincticeps, Nilaparvata lugens, Pseudococcus comstocki, Unaspis yanonensis, Myzus persicae, Aphis pomi, Aphis gossypii, Rhopalosiphum pseuddobrassicas, Stephanitis nashi, Nazara spp., Cimex leclularius, Trialeurodes vaporariorum, Psylla spp., Blatella germanica, Periplanela americana, Gryllotalpa africana, Locusta migratoria migratoriodes, Reticulitermes speratus, Coptotermes formosanus, Thrips palmi karny, Musaca domestica, Aedes aegypti, Hylemia platura, Culex piptens, Anopheles sinensis, Culex lrilaeniorhynchus, Tetranychus telarius, Panonychus citri, Aculops pelekassi, Tarsonemus spp., Meloidogyne incognita, Bursaphelenchus lignicolus mamiya et kiyohara, Aphelenchoides bessey, Heterodera glycines, Pratylenchus spp., etc. or any related species, such as a species within the same genus or family.
3. Timing
The treatments disclosed herein can be started, stopped, modified, paused, etc. based on a predetermined or programmable schedule and/or a trigger. In some instances, watering, weeding, fertilizing, calendar days, days of the week, time of the day or night, exposure to light, sensors, stage of a plant life, crop cycle, etc. can be used as a trigger. Stages of a plant life include seed, germination, growth, reproduction, pollination, spreading seed, fruiting, harvest, etc. In some instances, environmental changes can be a trigger, such as, but not limited to, air or ground temperature, rain, cloud cover, approaching storm, passage of a storm, change in electromagnetic field such as those that occur with storms, ion concentration, concentration of a chemical or compound, etc.
4. Location and Environmental Modifications
One or more treatment(s) can be applied to different portions of a plant and/or seed, or the surrounding environment (s), to produce a specific result. In a non-limiting example, two or more radiating structures may be simultaneously used on the lower and upper portions of the plant. In this non-limiting example, one recipe may be applied to the root system, to potentiate nutrient acquisition while the same or different recipe(s) may be simultaneously applied to the stem and leaves of the plant to increase cannabinoid production. Different portions of the plant can include, but are not limited to, any part of the root system, including course roots, fine roots, adventitious roots, and root hairs, and any part of the shoot system, including stems, leaves, branches, flowers, inflorescences, bark, internodes, or any other part or organ considered to be a part of the plant and/or seed. Said applications can further be applied to any part of the surrounding environment including the surrounding atmosphere, rhizosphere, water or nutrients that may come in contact with the plant and/or seed in order to alter the surrounding environment and/or environmental properties.
These and other non-limiting aspects of the present invention are discussed in further detail in the following sections.
The present invention will be described in greater detail by way of specific examples. The following examples are offered for illustrative purposes only, and are not intended to limit the invention in any manner. Those of skill in the art will readily recognize a variety of noncritical parameters, which can be changed or modified to yield essentially the same results.
When applied to Cannabis sativa, L., electromagnetic treatments described in Table 1. increased the overall concentrations of both cannabinoids and terpenes. The following cannabinoids were measured: cannabidiol (CBD), cannabidiolic acid (CBDa), cannabidiolic acid (CBDa) cannabinol (CBN), tetrahydrocannabivarin (THCV), tetrahydrocannabivarin acid (THCVa), tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCa), cannabichromene (CBC), cannabichromene (CBC), cannabichromenic acid (CBCa), cannabigerol (CBG), and cannabigerolic acid (CBGa). It is understood that certain traits of other cannabinoids, terpenes, and plant compounds are substantially similar to the cannabinoids and terpenes disclosed herein, and that said systems and treatments apply to additional cannabinoids, terpenes, and plant compounds, including but not limited to charged/uncharged variants, structural variants, and structurally or functionally related compounds. When the aforementioned cannabinoids were compared between eight (8) plants that received the electromagnetic treatment and eight (8) plants that did not receive the treatment, for a total of sixteen (16) genetically-identical clones from a cultivar, impacts were noted in overall cannabinoids, and in individual cannabinoids including tetrahydrocannabinol (THC), tetrahydrocannabinolic acid (THCa) and cannabichromenic acid (CBCa). See Table 2. The cannabidiolic acid (CBDa) and cannabigerol (CBG) concentrations decreased while the cannabinol (CBN) and tetrahydrocannabivann (THCV) were effectively unchanged. The increase in tetrahydrocannabinol (THC) and coinciding decrease in both cannabidiol (CBD) and cannabigerol (CBG) demonstrates the utility of this technology for altering the ratio of these molecules (which share a biosynthetic pathway). The overall impact in cannabinoids demonstrate the utility of this technology for impacting the overall amount of cannabinoids and the cannabinoid profile. These results demonstrate the utility of the described technology to increase and/or alter the relative abundance of cannabinoids, including tetrahydrocannabinol (THC), which are economically valuable due to their therapeutic and recreational applications. These results also demonstrate the utility of impacting the ratios of different cannabinoids and altering the overall cannabinoid profile of a cultivar, impacting the “entourage effect” or individual or group response(s) and experience(s) to the combined effects of multiple biologically active compounds sharing the same or related biochemical receptors.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
          
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
        
      
    
  
The electromagnetic treatments, when applied to the eight (8) genetically identical plants referenced above, resulted in a substantial increase in terpene concentrations relative to plants that did not receive electromagnetic treatments. Concentrations from the following terpenes were measured: α-Pinene, β-Pinene, β-Caryophyllene, Humulene, Limonene, Linalool, Camphene, and Myrcene. Concentrations of all of the aforementioned terpenes increased with the exception of Humulene, which decreased in concentration by four-point-two (4.2) percent. See Table 3. Increases in α-Pinene, β-Pinene, Limonene, linalool, Camphene, and Myrcene ranged from seventeen-point-nine (17.9) to thirty-three-point-nine (33.9) percent. Total terpene abundance was on average 10.1% higher in the plants that received the treatment compared to plants that did not receive the treatment. Camphene was expressed in detectable levels in five (5) of the eight (8) treated plants and only two (2) of the eight (8) plants that did not receive a treatment.
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
          
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
        
      
    
  
In one experiment comparing 27 Cannabis sativa plants, the plant that received the electromagnetic treatment described in Table 4 exhibited a 27.05% total abundance of measured cannabinoids, compared to the average of 22.3% in plants that did not receive the treatment, resulting in 21.4% greater total cannabinoids measured in comparison to the average of plants that did not receive the treatment. In this case, the plant that received the electromagnetic treatment produced a greater amount of cannabinoids than all other plants, which is commercially desirable.
In the experiment comparing 27 Cannabis sativa plants, the plant that received the electromagnetic treatment described in Table 4 exhibited a 3.07% total abundance of measured terpenes, compared to the average of 2.37% in plants that did not receive the treatment, resulting in 29.5% greater total terpenes measured in comparison to the average of plants that did not receive the treatment. In this case, the plant that received the electromagnetic treatment produced a greater amount of terpenes than all other plants, which is commercially desirable.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatments can increase and/or alter yield. When electromagnetic treatments described in Table 5 were applied to three (3) separate Cannabis sativa L. cultivars dried flower biomass yield increased by twenty-seven (27), twenty-three (23), and thirty-four (34) percent for each of the cultivars. See 
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatments can increase and/or alter harvest index. When electromagnetic treatments of Table 5 were applied to three (3) separate Cannabis sativa L. cultivars (
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
        
      
    
  
An electromagnetic recipe as described in Table 7, when applied to two (2) Cannabis sativa L. cultivars different from those previously discussed, demonstrated that electromagnetic treatments can lower and/or alter the ratio. See Table 8. The percent change (% change) indicates the difference between the treated and the control groups within each cultivar. There were five (5) individual plants in each of the treated and control groups for cultivar 4 and 12 individual plants in each of the treated and control groups for cultivar 5.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
            
          
          
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
          
        
      
      
        
        
        
        
        
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
            
            
            
          
          
            
          
        
      
    
  
Electromagnetic treatment can improve and/or alter maturation, early vigor, and time elapsed between different points in lifecycle development. When an electromagnetic treatment as described in Table 9 was applied to Zea mays, pre-germination and pre-sowing, the time to reach germination (
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Further efficacy was observed when pre-treated seeds were exposed to cold stress of 5 degrees C. for 7 days post treatment in Zea Mays. When 94 seeds were pre-treated with the electromagnetic treatment as described in Table 10 and subsequent cold stress described herein, the treated seeds exhibited greater rates of germination (
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 11 were applied to Zea mays seed, biomass yield increased by five (5) percent. Sample size was approximately 2,890 plants each for treated and control.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 13 were applied to Zea mays seed, biomass yield increased by seven (7) percent. Sample size was approximately 2,890 plants each for treated and control.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 15 were applied to Zea mays seed, biomass yield increased by five (5) percent. Sample size was approximately 2,890 plants each for treated and control.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 17 were applied to Eruca vesicaria (arugula) seed, biomass yield increased by five (5) percent. Sample size was 16 treated specimen trays and 16 control specimen trays.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 19 were applied to Brassica rapa (mizuna) seed, biomass yield increased by seven (7) percent. Sample size was 16 treated specimen trays and 16 control specimen trays.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 21 were applied to Zea mays seed, vegetative growth mass increased by nine (9) percent. Sample size was 140 treated specimen and 140 control specimen.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
Electromagnetic treatment can increase and/or alter yield. When an electromagnetic treatment as described in Table 23 were applied to Zea mays seed, vegetative growth mass increased by ten (10) percent. Sample size was 140 treated specimen and 140 control specimen.
  
    
      
        
        
          
            
          
          
            
          
          
            
          
          
            
          
        
        
          
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
  
    
      
        
        
          
            
          
        
        
          
            
          
          
            
          
        
      
      
        
        
        
          
            
            
          
          
            
            
          
        
      
      
        
        
        
        
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
            
          
          
            
            
          
        
      
    
  
This application claims the benefit of priority to U.S. Provisional Patent Application Ser. No. 63/114,889, filed Nov. 17, 2020, hereby incorporated by reference in its entirety.
| Filing Document | Filing Date | Country | Kind | 
|---|---|---|---|
| PCT/US2021/059738 | 11/17/2021 | WO | 
| Number | Date | Country | |
|---|---|---|---|
| 63114889 | Nov 2020 | US |