The present disclosure relates to a system for efficiently manipulating small scale fibers such as nanofibers or microfibers, and more specifically to a system that uses acoustic holograms to manipulate the fibers as they are being formed.
Electrospinning is a technique for producing polymer fibers with unique properties and applications in various fields. More specifically, electrospinning is a small-scale fiber fabrication process driven by a high voltage system, used to produce micro and nano size polymeric fiber structures that have high surface area, porosity, and flexibility when compared to other materials. In conventional electrospinning setups, a syringe containing a polymer solution is positioned opposite a grounding plate. Under electrostatic force, the polymer is pulled towards the grounding plate which collects these fibers. During the ejected jet's “flight” through space to the grounding plate, the ejected jet elongates in plastic deformation before solidifying into a solid fiber and depositing onto the collection plate. The fibers can be further processed after the electrospinning process for use in industries such as biomedical engineering, tissue engineering, regenerative medicine, and textiles. Current electrospinning systems lack a suitable system for guiding the fibers during travel, so there is a need in the art for an improved system and method of guiding these fibers during travel between the syringe and the grounding plate.
In an aspect, a system for forming a polymer fiber is disclosed. The system comprises a reservoir for holding a polymer solution, an emitter for emitting a polymer jet of the polymer solution from the reservoir whereby the polymer solution solidifies into a fiber, and a fiber guidance system. The fiber guidance system comprises a phased array of transducers and a control system. The control system is configured to direct the phased array of transducers to generate acoustic energy for creating an acoustic hologram operative to guide the polymer jet in space as the polymer jet is emitted from the emitter such that the fiber is formed to correspond with the acoustic hologram generated by the phased array of transducers.
Another aspect of the present disclosure relates to the creation of a pattern of air pressure including at least one low-pressure guide channel within the fiber guidance system. The system emits at least one polymer jet along the low-pressure guide channel. The low-pressure guide channel has a width that is determined by the holographic aperture that is generated by the phased array of transducers. The generated acoustic hologram and the resulting pattern of air pressure may dynamically manipulate the polymer jet to produce a fiber that is twisted, cabled, cylindrical, flat, hollow, or porous. In some cases, the phased array of transducers may be arranged circumferentially about an emission axis of the polymer jet. In other cases, the phased array of transducers may be arranged in rectangular or hexagonal arrangement about an emission axis. Furthermore, multiple emitters may be used with a phased array of transducers to increase the output of fibers. A phased array of transducers may be controlled to generate many acoustic holograms within the fiber guidance system, guiding multiple polymer jets over a travel distance to the grounded collector.
Other aspects and features will be apparent hereinafter.
Corresponding parts are given corresponding reference numbers throughout this disclosure.
One challenge with electrospinning is fiber guidance. Conventional electrospinning techniques produce an essentially random structure of fibers on the grounding plate. But to maximize the utility of the fibers, it would be beneficial to have a mechanism for guiding the fibers as they travel from the emitter toward the grounding plate. This would facilitate the formation of fiber materials with improved fiber morphologies, which could have numerous applications in many industries. A fiber guidance system coupled with electrospinning mechanisms makes it possible to produce fibers with different morphologies by utilizing acoustic hologram patterns to alter the structure of the fiber. Furthermore, the fiber guidance system may increase the capacity to output nanofiber or microfiber material on an industrial scale by controlling the deposition of the fibers and the uniformity of the fibers. For example, the directed deposition of the fibers can achieve the desired porosity of electrospun mats which is critical for tissue engineering, filtration membranes, and drug delivery systems.
Referring now to
In the presently described embodiment, the system 10 comprises a reservoir 14 for holding polymer solution, an emitter 16 for emitting a jet of the polymer solution from the reservoir, and a grounded collector 18 (e.g., a plate with a grounded metal surface) on which the polymer collects after solidifying into a fiber material. The fiber guidance system 12 comprises a phased array of transducers 20 and a control system 22 configured to direct the array of transducers to generate acoustic energy for creating an acoustic hologram (e.g., a pattern of acoustic energy made up of interfering acoustic signals) along the path of the polymer jet from the emitter 16 to the collector 18. The acoustic hologram created by the fiber guidance system 12 guides the polymer jet in space as the polymer jet is emitted from the emitter 16 toward the collector 18. The polymer jet naturally undergoes plastic stretching and is further manipulated by the fiber guidance system 12 as it travels to the collector 18. Thus, the fiber is formed to have a structure that corresponds with the acoustic hologram generated by the phased array of transducers.
In general, the emitter 16 is configured to emit the jet of polymer solution along an emission axis EA toward the collector 18. In an exemplary embodiment, the emitter 16 is electrically charged through application of an electric field with high voltage. The application of the electric field may vary with factors such as material viscosity and travel distance. In some cases, for example, the voltage applied may be between 10-130 kV, however other voltages may be used without departing from the scope of the present disclosure. When the applied electric field strength overcomes the surface tension of the solution at the tip of the emitter 16, a polymer jet is emitted from the emitter 16.
In the illustrated embodiment, the reservoir 14 comprises a syringe barrel and the emitter 16 comprises a capillary tip. In one or more embodiments, the system 10 comprises a driver 24 configured to apply an electrostatic force to the emitter 16 to drive emission of the jet of polymer solution. In
To produce fibers, various polymer solutions may be employed. These solutions typically comprise a polymer dissolved in a solvent. Common polymers utilized include, but are not exclusively limited to, polyethylene oxide (PEO), polyvinyl alcohol (PVA), polycaprolactone (PCL), polyacrylonitrile (PAN), polyvinylidene fluoride and poly(lactic-co-glycolic acid) (PLGA), each offering distinct advantages in terms of bio-compatibility and mechanical strength. The solvents used in the polymer solution must effectively dissolve the polymer and evaporate at a rate that makes it possible to effectively form fibers. Examples of solvents include water, ethanol, chloroform, acetone and dimethylformamide (DMF).
Turning now to the fiber guidance system 12 as shown in
As explained more fully below, the control system 22 is configured to control the ultrasonic transducers 20 to generate an acoustic hologram (e.g., a pattern of interference between the acoustic waves output by the transducers), which creates a pattern of air pressure including at least one low-pressure guide channel 35. That is, the transducers 20 are controlled so that the ultrasonic waves output from the array interfere in a predetermined pattern creating static or dynamic acoustic holograms that may change along the length of the polymer jet travel path. Those skilled in the art will recognize that interference between acoustic waves can be used to establish a corresponding pattern of air pressure in space, e.g., alternating regions of low and high pressure. In this disclosure, the fiber guidance system 12 uses the correlation between acoustic wave interference and air pressure to form one or more low pressure channels inside the array. The system 10 is configured so that the emitter 16 emits the polymer jet along a low-pressure guide channel 35 and the polymer jet follows the low-pressure guide channel 35. In this way, the acoustic hologram generated by the array is capable of guiding the polymer jet in space. Further, the fiber material that collects on the collector 18 forms a structure that corresponds to the acoustic hologram because of the effect the acoustic hologram had on the polymer jet in flight.
As shown, the phased array of transducers 20 preferably comprises a plurality of transducer layers stacked along the emission axis EA. Each layer comprises n transducers circumferentially spaced apart about the emission axis EA in spacing increments of n/360 degrees. In certain embodiments, each transducer layer includes at least 12 transducers (n≥12) circumferentially spaced apart about the emission axis EA. In a presently preferred embodiment, each transducer layer comprises 96 transducers circumferentially spaced apart about the emission axis EA. In the illustrated embodiment, the transducers 20 in adjacent layers are circumferentially offset from one another such that the array defines an alternating honeycomb pattern. Suitably, each layer of transducers can be mounted on an appropriately designed printed circuit board. In an example, an appropriately designed printed circuit board comprises a plastic frame configured to align the transducers 20 on the printed circuit board 100 such that the transducers 20 are mounted and soldered to the side of the printed circuit boards 100. As explained in further detail below, the printed circuit boards 100 are configured to connect the control system 22 to each of the transducers 20. An exemplary embodiment of a printed circuit board 100 for a fiber guidance system 12 is shown in
Referring to
In an exemplary embodiment, each microcontroller 40 comprises a gate driver 42 connecting the respective subset of the transducers 20 to the respective microcontroller and a transducer power supply 26. Each microcontroller 40 is configured to signal the respective gate driver 42 to actuate the transducers 20 with power from the transducer power supply 26. The illustrated control system 22 further comprises a synchronization system 44 connected to all of the microcontrollers 42. The synchronization system 44 is configured to synchronize the activation frequencies of the microcontrollers 40 so that the microcontrollers fire the respective transducers 20 at the correct time to generate the desired acoustic hologram. The synchronization system 44 is configured to continuously execute a synchronization feedback loop with each of the microcontrollers 40 to ensure that the synchronization frequencies of the microcontrollers remain calibrated and accurate
In general, the control system 22 is configured for adjusting the phase of the ultrasound wave output from each transducer 20 so that the transducers collectively create the desired acoustic hologram. In one example, the phase of a transducer 20 is based on the angle formed between that transducer, the center of the array (e.g., the emission axis EA), and a transducer of the same layer chosen to be 0 degrees for the layer. In the case of an array of transducers set so that there are 96 transducers per layer and wherein the layers are laid out in an alternating honeycomb pattern, the zero-position transducer for each layer is incremented by 360/192 degrees for each double layer from the bottom because there are 192 transducer positions about the emission axis EA. In this example there are fifteen double layers of stacked transducers.
Using the control system 22, the phase of the ultrasound wave outputted from each transducer 20 can be adjusted to create an acoustic hologram which creates a sufficiently defined pattern of air pressure to noticeably alter the typically random path of polymer jet emission. The transducers 20 facing each other produce an interference pattern, and when in phase, a high pressure is produced at the center point. Adjusting one transducer to be out of phase by 180° from an oppositely positioned transducer, produces a low-pressure channel and modulating the phase over time produces a dynamic acoustic hologram. Within the low-pressure channel, the polymer jet is guided to the collector as the combination of stretching and solvent evaporation causes the polymer jet to solidify into a fiber. It is possible to vary the holographic aperture by changing the Vortex Number, which in turn, alters the width of the low-pressure channel. The Vortex Number indicates how many 360 degree phase rotations are completed by the phased array of transducers around the perimeter of the low-pressure channel. The ring of the of transducers 20 may be divided by radial segmentation into sectors. The Vortex Number increases with the number of sectors and the number of phase changes within the ring which results in an increase of the diameter of the holographic aperture. Contrariwise, decreasing the Vortex Number constricts the holographic aperture and reduces the size of the low-pressure channels.
The system 10 guides the emitted polymer jet to generally follow the path of the low-pressure guide channel(s) 35 created by the dynamic interference of ultrasonic waves. Accordingly, the system 10 can be employed to create structured fiber material that is guided by the acoustic hologram, whereby the fiber material has a structure that is not random, but rather has characteristics that correspond to the acoustic hologram. Furthermore, the density and alignment of the fibers collected may be modified with the fiber guidance system 12, by altering the acoustic hologram and precisely controlling the feed rate of the emitter 16. The system 10 can be configured to produce microfibers, nanofibers and fiber materials of various desired shapes. For example, in one or more embodiments, the system 10 is configured (by adjusting the acoustic hologram) to produce twisted fibers. Those skilled in the art will appreciate that twisted fibers (e.g., twisted nanofibers or microfibers) would be useful for applications such as tissue scaffolding because they have extremely high surface area. Different morphological structures may be created such as smooth cylindrical fibers, flat fibers, porous fibers, hollow fibers and bead-on-string structures. The fiber diameter may range from nanometers to micrometers.
The system 10 can be scaled to produce greater outputs of fiber material. For example, instead of using a cylindrical array of ultrasound transducers 20, the system could use a rectangular or hexagonal array. An exemplary embodiment of a rectangular phased array of transducers is shown in
Turning now to
One strategy of scaling the system 10, involves increasing the quantity of transducers in the array, in an example, a circular array of 192 transducers in each layer may be employed. Depending on the scale of the equipment and the production needs, thousands of emitters may be employed within a phased array to increase the output of fibers. In one larger scale embodiment, 3,600 emitters are employed with 21,000 transducers within a phased array. Further, multiple emitters with the larger phased array may produce a cabled fiber without any additional post processing of the fibers. The emitters may be placed upon a robotic head or gantry system to more accurately place and move the emitters for directed deposition and fiber manipulation within the phased array.
It is envisioned that any number of layers of transducers 20 may be employed to generate an acoustic hologram over a distance adequate to shape the polymer jet as it is traveling from the emitter 16. Additionally, the system may employ high-frequency arrays to achieve a reduction in acoustic wavelength. These high frequency arrays may be configured to operate within a frequency spectrum spanning from approximately 300 kHz-1 MHz. It should be understood that this frequency range is illustrative and non-limiting, as the system may be adapted to utilize frequencies outside this range as necessitated by specific application requirements or technological advancements.
Referring to
Turning now to the experimental efforts to produce fibers with the system 10, two examples of fiber production are discussed. In the field of electrospinning, the presence of beads along fiber lengths is generally considered a defect. These beads are undesirable because they introduce inconsistencies in fiber morphology and can alter the mechanical properties of the resultant fibers. Smooth, uniform fibers are typically preferred. The fiber guidance system 12 has shown an improvement in fiber morphology, including the elimination of beads during experimentation.
In the second test, a polymer solution with 10% polyvinylidene fluoride, 63% dimethylformamide, and 27% acetone was placed on a magnetic stirrer at 450 rpm for 4 hours at 55° C. The expressing actuator was set to 1 mL/hr. The electrospinning process was conducted with the phased array of transducers set to 20V. In both cases, the nanofiber material M1, M2 appears as a whitish residue on a circular foil sheet. The foil was placed on the collector during each respective test. As can be seen, the material M2 is more structured that the material M1. Whereas the material M1 is randomly distributed across most of the foil sheet, the material M2 is focused on two relatively small areas in the center of the plate. The location of the fiber deposition changed after several minutes. In the second test, nanoscale and uniform fibers were observed with no beads.
The inventor believes this focused formation of structured nanofiber material M2 occurred because the jet of polymer was guided through a low-pressure guide channel created by the acoustic hologram produced by the fiber guidance system. Additionally, the uniformity of the fibers improved and the beads of polymer were eliminated by using the fiber guidance system during electrospinning. The location of fiber deposition changed during the second test due to noise in synchronization signal disrupting normal operation of the phased array of transducers, resulting in irregularities in the acoustic hologram.
In the second test the same polymer solution preparation and expressing actuator settings were used, and in this case, the phased array of transducers was used during the electrospinning process. The images in
As seen in
When introducing elements of the present disclosure or the preferred embodiment(s) thereof, the articles “a”, “an”, “the” and “said” are intended to mean that there are one or more of the elements. The terms “comprising”, “including” and “having” are intended to be inclusive and mean that there may be additional elements other than the listed elements.
In view of the above, it will be seen that the several objects of the disclosure are achieved and other advantageous results attained.
As various changes could be made in the above products and methods without departing from the scope of the disclosure, it is intended that all matter contained in the above description shall be interpreted as illustrative and not in a limiting sense.
The present application claims the priority benefit of U.S. Provisional Patent Application Ser. No. 63/513,915, filed Jul. 17, 2023, entitled Systems and Methods for Nanofiber Guidance, which is hereby incorporated by reference in its entirety.
Number | Date | Country | |
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63513915 | Jul 2023 | US |