Patent Application
20190276403
Provided herein is t-butyl 2-carbamothioyl-2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate and processes of preparation.
U.S. Patent Application Ser. No. 62/163,106 describes inter alia certain metalloenzyme inhibitor compounds and their use as fungicides. The disclosure of this application is expressly incorporated by reference herein. This patent application describes various routes to generate metalloenzyme inhibiting fungicides. It may be advantageous to provide more direct and efficient methods for the preparation of metalloenzyme inhibiting fungicides and related compounds, e.g., by the use of reagents and/or chemical intermediates which provide improved time and cost efficiency.
Provided herein is the compound t-butyl 2-carbamothioyl-2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (I), which is useful for preparing certain metalloenzyme inhibitor compounds, and processes for its preparation. In one embodiment, provided herein, is a process for the preparation of the compound of the Formula I:
which comprises contacting a compound of Formula II with an organic isothiocyanate and then with a cleaving reagent.
In another embodiment, the compound of Formula II may be prepared by contacting a compound of Formula III with t-butyl carbazate.
Another aspect of the present disclosure are the novel intermediates produced in the present process, viz., the compounds consisting of:
The term “halogen” or “halo” refers to one or more halogen atoms, defined as F, Cl, Br, and I.
The term “organometallic” refers to an organic compound containing a metal, especially a compound in which a metal atom is bonded directly to a carbon atom.
Room temperature (RT) is defined herein as about 20° C. to about 25° C.
Throughout the disclosure, references to the compounds of Formulas I-III (including Ia and Ib) are read as also including optical isomers and salts. Specifically, when compounds of Formulas I-III contain a chiral carbon, it is understood that such compounds include optical isomers and racemates thereof. Exemplary salts may include: hydrochloride salts, hydrobromide salts, hydroiodide salts, and the like.
Certain compounds disclosed in this document can exist as one or more isomers. It will be appreciated by those skilled in the art that one isomer may be more active than the others. The structures disclosed in the present disclosure are drawn in only one geometric form for clarity, but are intended to represent all geometric and tautomeric forms of the molecule.
The embodiments described above are intended merely to be exemplary, and those skilled in the art will recognize, or will be able to ascertain using no more than routine experimentation, numerous equivalents of specific processes, materials and procedures. All such equivalents are considered to be within the scope of the invention and are encompassed by the appended claims.
t-Butyl 2-carbamothioyl-2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (I) may be prepared from t-butyl 2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (II) as shown in Example 1.
Method A:
To t-butyl 2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (II) (5 g, 9.39 mmol) in THF (31.3 mL) at 0° C. was added benzoyl isothiocyanate (1.199 mL, 8.92 mmol). After 30 min additional benzoyl isothiocyanate (0.1 mL, 0.74 mmol) was added. The benzoyl intermediate Ia was identified by LCMS (ESIMS m/z 696.1 [(M+H)+]). After an additional 30 min, anhydrous hydrazine (1.47 mL, 46.9 mmol) was added. The mixture was stirred at 0° C. for 1 h then room temperature for 30 min. The reaction was diluted with ethyl acetate and washed with sat. ammonium chloride. The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated to a pale yellow oil. Methanol (25 mL) was added to the oil and after a few minutes of stirring a white precipitate had formed. The slurry was filtered and the solid rinsed with methanol giving t-butyl 2-carbamothioyl-2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (I) (4.29 g, 7.25 mmol, 77% yield) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ 8.76 (s, 1H), 8.45 (d, J=11.9 Hz, 2H), 7.96-7.86 (m, 2H), 7.70 (dd, J=8.6, 2.8 Hz, 2H), 7.58 (d, J=8.4 Hz, 1H), 7.53-7.40 (m, 1H), 7.22-7.15 (m, 2H), 7.12 (t, J=11.0 Hz, 1H), 7.01 (d, J=8.8 Hz, 1H), 6.37 (s, 1H), 5.45 (d, J=15.7 Hz, 1H), 4.47 (d, J=15.3 Hz, 1H), 1.40 (s, 9H). 19F NMR (376 MHz, DMSO-d6) δ −104.72 (d, J=122.8 Hz), −107.49-−109.12 (m), −111.08-−111.85 (m). ESIMS m/z 592.2 [(M+H)+].
To a solution of t-butyl 2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (II, 1 g, 1.596 mmol) in ethyl acetate (9.4 mL) was added isothiocyanatotrimethylsilane (0.540 mL, 3.83 mmol) and the reaction was stirred at 80° C. for 18 h. NMR indicated incomplete conversion so additional isothiocyanatotrimethylsilane (0.540 mL, 3.83 mmol) was added and the reaction stirred at 80° C. for 6 h. NMR indication the reaction was still incomplete so more isothiocyanatotrimethylsilane (0.540 mL, 3.83 mmol) was added and the reaction stirred at 80° C. for 17 h. The reaction was allowed to cool to room temperature and 1 N HCl (10 mL) was added. The phases were separated and the organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated to a yellow foam. The yellow foam was dissolved in methylene chloride and purified by silica gel column chromatography eluting with 0-60% ethyl acetate/hexanes. Product containing fractions were collected and concentrated giving t-butyl 2-carbamothioyl-2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (I) as a yellow foam (460 mg, 0.778 mmol, 49% yield). Analytical data was consistent with that of previously obtained samples.
Organic isothiocyanates for use in this process step may include acyl isothiocyanates such as, for example, benzoyl isothiocyanate (to make the compound of Formula Ia), and silyl isothiocyanates such as, for example, trimethylsilyl isothiocyanate (to make the compound of Formula Ib).
Cleaving reagents used to remove the R-group from the compound of Formula Ia to prepare the compound of Formula I, may be selected from the group including hydrazine, ammonia, sodium methoxide, and methylamine. Cleaving reagents used to remove the R-group from the compound of Formula Ib to prepare the compound of Formula I, may be selected from: a) fluoride compounds such as, for example, a tetraalkylammonium fluoride and potassium fluoride, and b) an acid such as, for example, hydrochloric acid (HCl), hydrobromic acid (HBr), or sulfuric acid (H2SO4).
The contacting of the compound of Formula II with the organic isothiocyanate may be carried out between about −20° C. and about 100° C., and the contacting with the cleaving reagent may be carried out between about −20° C. and about 100° C.
Solvents for use in this process step may include one or more than one of THF (tetrahydrofuran), EtOAc, 2-Me-THF, dioxane, MeCN (acetonitrile), and DME (1,2-dimethoxyethane).
t-Butyl 2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (II) may be prepared from 4-((6-((2-(2,4-difluorophenyl)oxiran-2-yl)difluoromethyl)pyridin-3-yl)oxy)benzonitrile (III) as shown in Example 2.
To a slurry of 4-((6-((2-(2,4-difluorophenyl)oxiran-2-yl)difluoromethyl)pyridin-3-yl)oxy)benzonitrile (III) (5 g, 12.49 mmol) in ethanol (40 mL) was added t-butyl carbazate (4.13 g, 31.2 mmol) and the reaction was heated at 80° C. for 24 h, at which point the starting epoxide (III) was completely consumed. The reaction was allowed to cool to 45° C. and seeded with crystals of product II causing the reaction to cloud. Additional ethanol (40 mL) was added, and the reaction was cooled to room temperature overnight. The resulting slurry was cooled with an ice bath for 30 min and filtered. The solids were rinsed with ethanol (30 mL) and dried under vacuum providing t-butyl 2-(3-(5-(4-cyanophenoxy)pyridin-2-yl)-2-(2,4-difluorophenyl)-3,3-difluoro-2-hydroxypropyl)hydrazine-1-carboxylate (II) as a white solid (5.42 g, 9.67 mmol, 77% yield). 1H NMR (400 MHz, CDCl3) δ 8.37 (d, J=2.7 Hz, 1H), 7.72-7.64 (m, 2H), 7.55 (td, J=8.8, 6.6 Hz, 1H), 7.48 (d, J=8.6 Hz, 1H), 7.37 (dd, J=8.7, 2.7 Hz, 1H), 7.10-7.02 (m, 2H), 6.77 (dddd, J=20.9, 11.4, 8.6, 2.6 Hz, 2H), 3.83 (d, J=13.7 Hz, 1H), 3.74 (dd, J=13.4, 2.8 Hz, 1H), 1.41 (s, 9H). 19F NMR (376 MHz, CDCl3) δ −105.15, −108.68 (d, J=22.1 Hz), −109.24, −110.29. ESIMS m/z 533.1 [(M+H)+].
The contacting of the compound of Formula III with t-butyl carbazate may be carried out from about 25° C. to about 100° C. or from about 60° C. to about 90° C.
Solvents for use in this process step may include alcohols such as methanol, ethanol, and isopropanol, as well as aprotic solvents such as THF (tetrahydrofuran), acetonitrile, DMSO (dimethylsulfoxide), DMF (N,N-dimethylformamide), and mixtures of any of these solvents.
The present application claims priority under 35 U.S.C. § 119(e) to U.S. provisional patent application, U.S. Ser. No. 62/423,858, filed Nov. 18, 2016, the entire contents of which is incorporated herein by reference.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2017/062147 | 11/17/2017 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 62423858 | Nov 2016 | US |
