Telomerase-associated proteins

Abstract

A human staufen polypeptide that is associated with telomerase is described. Methods for inhibiting telomerase activity also are described.

Description

TECHNICAL FIELD

The invention relates to a human staufen polypeptide that is associated with telomerase.

BACKGROUND OF THE INVENTION

Telomerase is a specialized ribonucleoprotein (RNP) reverse transcriptase that is essential for telomere maintenance. Telomerase uses an internal RNA template to synthesize telomeric repeat sequences onto chromosome ends. Deletion of the essential RNA component of telomerase leads to progressive telomere shortening, chromosome instability and cell death in both yeast and mouse cells. This multi-subunit enzyme is regulated at several levels in human cells.

The telomerase enzyme is made up of an essential core as well as several accessory proteins. The core telomerase consists of the RNA component (Telomerase RNA, TR) and the catalytic subunit (Telomerase Reverse Transcriptase, TERT). The structure of the RNA component is conserved in ciliates, in which the RNA is 150-200 nucleotides (nt) in length. In mammalian cells, the RNA component is significantly larger, 390-450 nt. Feng, J. et al., Science, 1995, 269:1236-1241. The catalytic TERT component, first identified in the ciliate Euplotes, has homologues in yeast (EST 2), human (hTERT), and mouse (mTERT). TERT contains sequence motifs similar to reverse transcriptase and mutations of essential aspartate residues that are conserved in the catalytic triad of reverse transcriptases eliminates telomerase activity. Minimal telomerase activity can be reconstituted in an in vitro transcription/translation extract using TERT and TR components, indicating that these are sufficient for catalysis. Weinrich, S. L. et al., Nat. Genet., 1997, 17:498-502.

Both telomere length and telomerase activity have been implicated in cellular senescence and cancer. In most somatic cells, telomerase activity is not detected and telomeres shorten with each division. Allsopp, R. C. et al., Proc. Natl. Acad. Sci. USA, 1992, 89:1014-1018. Artificial elongation of telomeres by ectopic hTERT expression in primary human cells leads to telomere elongation and a bypass of cellular senescence, suggesting that telomere shortening may trigger cellular senescence in primary human cells. During immortalization of mammalian cells in culture, telomerase is activated, telomere length is stabilized, and cells continue to proliferate, suggesting that telomerase activation and telomere stabilization are required for the long term growth of cancer cells. Telomerase activity is present in the vast majority of human tumors while little activity is found in the normal tissues from which the tumors were derived. Together, these data lead to the proposal that telomerase inhibition may inhibit tumor growth. See, for example, Harley, C. B. et al., Cold Spring Harbor Symp. Quant. Biol., 1994, 59:307-315.

New evidence also indicates that telomerase plays a role in tumor initiation, not just in long term tumor growth. Telomerase null mice with significantly shortened telomeres show an increased rate of tumor formation with age compared with wild-type mice, suggesting loss of telomere function leads to increased genetic instability. Furthermore, in cells lacking the p53 gene, absence of telomerase increased the rate of focus formation after transfection of myc and RAS. This suggests that the absence of both telomerase and p53 cooperated to increase genetic instability that leads to tumor initiation. Thus, characterization of telomerase and its associated components is important to understanding tumor formation.

SUMMARY OF THE INVENTION

The invention is based on the identification of telomerase-associated proteins that bind telomerase RNA and that may play a role in telomerase assembly, transport, and regulation. As described herein, human staufen (hStau) polypeptide and ribosomal associated protein L22 bind human telomerase RNA and are associated with telomerase activity in vivo.

In one aspect, the invention features an isolated human staufen polypeptide, wherein the polypeptide includes an amino acid sequence substantially identical to the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2. For example, the polypeptide can be substantially identical to the amino acid sequence of SEQ ID NO:2. The polypeptide also can be, for example, the amino acid sequence of SEQ ID NO:2 or the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2. Polypeptides that include the amino acid sequence of SEQ ID NO:2 can have a molecular weight of approximately 55 kDa. Polypeptides of the invention bind RNA. For example, the polypeptide can bind double-stranded RNAs such as telomerase RNA. The polypeptide can be in a complex with the catalytic subunit of telomerase.

The invention also features an isolated polynucleotide encoding a human staufen polypeptide. The encoded polypeptide can include an amino acid sequence substantially identical to the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2. For example, the polypeptide can include the amino acid sequence of SEQ ID NO:2. The polynucleotide can have a nucleotide sequence at least 70%, 80%, 90%, or 95% identical to the nucleotide sequence of SEQ ID NO:1.

Polynucleotides of the invention also can be at least 150 nucleotides in length and hybridize under stringent conditions to the nucleotide sequence of SEQ ID NO:1 or the complement thereof. For example, the polynucleotide can include the nucleotide sequence of SEQ ID NO:1.

In another aspect, the invention features an antibody having specific binding affinity for a human staufen polypeptide that includes, for example, the amino acid sequence of SEQ ID NO:2. The antibody can be polyclonal or monoclonal.

The invention also features a method for inhibiting telomerase activity. The method includes administering to a cell an amount of an agent effective to inhibit the interaction between a telomerase-associated polypeptide and telomerase. The telomerase-associated polypeptide can be, for example, human staufen polypeptide or ribosomal associated protein L22.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used to practice the invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

Other features and advantages of the invention will be apparent from the following detailed description, and from the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A and 1B are a structural alignment of hStau (TEP3) and other double stranded (ds) RNA binding proteins ( 1 A) and a sequence comparison of domains in hStau and Drosophila Staufen (dSTAU) ( 1 B). In FIG. 1A , full length dsRNA binding domains are indicated by gray boxes and short domains by white boxes. Numbers under the domains in hStau and dSTAU correspond to the sequences listed in FIG. 1 B. In the top panel of FIG. 1B , alignments of full length domains are shown. The bottom panel shows alignment of short domains. Identical residues are shaded. Asterisks (*) indicate the residues that are most highly conserved in all of the in the ds RNA binding domain containing proteins. Sequences used in the alignment are: hStau 1, aa 59 79 (TEP3-1)(SEQ ID NO:16); hStau 2, aa 100 172 (TEP3-2) (SEQ ID NO:17); hStau 3, aa 202 275 (TEP3-3)(SEQ ID NO:18); hStau 4, aa 452 472 (TEP3-4)(SEQ ID NO:19); dSTAU 1, aa 308 380 (SEQ ID NO:20); dSTAU 2, 490 559(SEQ ID NO:21); dSTAU 3, 575 647 (SEQ ID NO:22); dSTAU 4, aa 708 782 (SEQ ID NO:23); and dSTAU 5, aa 948 1020 (SEQ ID NO:24).

FIGS. 2A and 2B are Western blots that indicate hStau antibody ( 2 A) and L22 antibody ( 2 B) specificity. FIG. 2A is a Western blot analysis of 293 cell extract (lane 1) and hStau immunoprecipitates (lane 2) using anti-hStau antibody, that shows a 55 kD hStau protein. FIG. 2B is Western blot analysis of 293 cell extracts (lane 1) and L22 immunoprecipitates using pre-immune serum (lane 2), anti-L22 serum (lane 3), and using anti-L22 serum preincubated with L22 peptide before immunoprecipitation. Arrow indicates the L22 band (15 kD). The relative mobilities of molecular weight markers (in kD) are indicated to the left in FIGS. 2A-2B .

FIG. 2C is an RT-PCR analysis of RNAs in the supernatant (lane 1-7) and pellet (lane 8-14) fractions of hStau and L22 immunoprecipitation reactions. Lanes 1 and 8, hStau precipitation using pre-immune serum; lanes 2 and 9, precipitation using anti-hStau antibody; lanes 3 and 10, precipitation using anti-GST antibody; lanes 4 and 11, precipitation using anti-chymotrypsin antibody; lanes 5 and 12, precipitation using L22 pre-immune serum; lanes 6 and 13; precipitation using anti-L22 antibody; lanes 7 and 14, precipitation using anti-L22 antibody pre-incubated with L22 peptide. The RNAs amplified in the fractions are indicated on the left (hTR, U2, U3, 7SL, and RNase P).

FIG. 2D is a quantitation of RNAs precipitated in FIG. 2 C. The percentage of the RNA in the pellet is expressed as a fraction of the total signal intensity obtained in both pellet and supernatant. RNAs assayed are indicated on the left and antibodies used are indicated on the bottom.

FIGS. 3A and 3B are autoradiographs that indicate hStau and L22 interact with telomerase ( 3 A) and hTERT ( 3 B). In FIG. 3A , telomerase assays were carried out on 293 extract (lane 1) and immunoprecipitation pellets (lanes 2-13) from immunoprecipitations with various antibodies. Lanes 2 and 3, precipitation with hStau pre-immune serum; lanes 4 and 5, precipitation with anti-hStau antibody; lanes 6 and 7, precipitation with anti-GST antibody; lanes 8 and 9 precipitation with L22 pre-immune serum; lanes 10 and 11, precipitation with anti-L22 antibody; and lanes 12 and 13, precipitation with anti-L22 antibody pre-incubated with L22 peptides. Samples in lanes 3, 5, 7, 9, 11, and 13 were pretreated with RNase before telomerase reactions. Arrow indicates the location of the internal control (IC) for PCR amplification.

FIG. 3B is a Western blot analysis using anti-HA antibody on cell lysate (lanes 1 and 2) or various immunoprecipitation pellets (lanes 3-8). Extracts for lanes 1, 3, and 6 were mock transfected 293 extract, and were hTERT-HA transfected cell extract for lanes 2, 4, 5, 7, and 8. Antibodies used in immunoprecipitation reactions were: lanes 3 and 5, anti-L22; lane 4, L22 pre-immune serum; lanes 6 and 8, anti-hStau; lanes 7, hStau pre-immune serum. The relative mobility of molecular weight markers (in kD) are indicated to the left. Arrow indicates the hTERT-HA tagged band.

FIGS. 4A and 4B are Northern blots that indicate hStau mRNA expression in human tissues. Tissues from which RNAs were extracted are indicated at the top of the gel. The arrows indicate the sizes of the hStau (3.3 kb) and β-actin message (2.0 kb). For each gel, the amount of hStau relative to β-actin was quantified and the ratio is given for each lane at the bottom of the gel. Two filters were hybridized separately in different experiments, thus the values for hStau/actin ratio of the two gels can not be compared directly to each other.

FIGS. 5A-5C are Western blots that examine interactions between telomerase-associated proteins. FIG. 5A indicates that hStau and L22 do not interact with each other. 293 cell lysate was immunoprecipitated with hStau pre-immune serum (lane 2), hStau antibody (lane 3), L22 pre-immune serum (lane 4), or L22 antibody (lane 5). Pellet fractions were analyzed by western hybridization, and probed with either hStau antibody (upper panel) or L22 antibody (lower panel). FIG. 5B indicates that hStau and myc-TEP1 do not interact. 293 cells were either mock transfected (lanes 1, 3, and 4) or transfected with myc-TEP1 construct (lanes 2, 5, and 6), and cell lysates were either run directly on a western blot (lanes 1 and 2) or immunoprecipitated with either hStau pre-immune serum (lanes 3 and 5) or hStau antibody (lanes 4 and 6). The western blot was probed with anti-myc antibody (upper panel) or hStau antibody (lower panel). FIG. 5C indicates that L22 and myc-TEP1 do not interact. 293 cells were either mock transfected (lanes 1 and 3) or transfected with myc-TEP1 construct (lanes 2, 4, and 5), and cell lysates were either run directly on a western blot (lanes 1 and 2) or immunoprecipitated with either hStau pre-immune serum (lane 4) or L22 antibody (lanes 3 and 5). The western blot was probed with anti-myc antibody (upper panel) or L22 antibody (lower panel).

DETAILED DESCRIPTION

The invention relates to human staufen (hStau) polypeptides, as well as polynucleotides encoding hStau polypeptides, antibodies having specific binding affinity for hStau polypeptides, and methods for using hStau and other telomerase-associated proteins to inhibit telomerase activity.

As described herein, hStau is a specific double stranded RNA binding protein that is specifically associated with human telomerase RNA (hTR) in vivo, and can be classified with a family of proteins that contain multiple double stranded RNA binding domains. The structural organization of the RNA binding domains of hStau, as well as sequences within these domains, are most similar to the domains of Staufen protein from Drosophila. See, St. Johnston, D. et al., Cell, 1991, 66(1):51-63. In Drosophila, staufen specifically binds bicoid and oskar mRNA, and is necessary for the proper localization of these RNAs to the anterior and posterior of the oocyte, respectively. Drosophila staufen also binds to prospero mRNA and contributes to its localization to neuroblasts during development. In vitro, staufen binds directly to bicoid mRNA. In fact, a 76 amino acid fragment containing the double stranded RNA binding domain of Drosophila staufen is sufficient to bind RNAs that contain extensive secondary structure, such as U1, U2, and VA1, but not unstructured RNAs. See, St. Johnston, D. et al., Proc. Natl. Acad. Sci. USA, 1992, 89(22):10979-10983. Although Drosophila staufen appears to bind structured RNA with little specificity in vitro, RNA binding proteins do have higher affinity for their specific target in vivo. For example, hStau binds hTR as well as U2 and U3 snRNAs in vivo, but has no detectable association with 7SL or RNase P RNAs in vivo. Since hTR only is present at about 500 copies per cell, these results suggest that hStau proteins bind hTR in vivo. In vivo binding specificity may be achieved, at least in part, through conformation of the RNA and/or the association with other proteins in a RNP complex.

Human Staufen Polypeptides

The invention features an isolated hStau polypeptide that includes the amino acid sequence of SEQ ID NO:2 and fragments thereof, as well amino acid sequences that are substantially identical to SEQ ID NO:2 or fragments thereof. For example, a hStau polypeptide can include about residue 49 to about residue 496 of SEQ ID NO:2. The term “polypeptide” includes any chain of amino acids, regardless of length or post-translational modification. “Substantially identical” amino acid sequences include the referenced amino acid sequence, as well as sequences that contain substitutions in the amino acid sequence of SEQ ID NO:2 that retain the capacity to bind RNA. In general, conservative amino acid substitutions, i.e., substitutions of similar amino acids, are tolerated without affecting protein function. Similar amino acids can be those that are similar in size and/or charge properties. For example, aspartate and glutamate and isoleucine and valine are both pairs of similar amino acids. Similarity between amino acid pairs has been assessed in the art in a number of ways. Dayhoff et al., Atlas of Protein Sequence and Structure, 1978, 5(3):345-352, provides frequency tables for amino acid substitutions which can be employed as a measure of amino acid similarity.

Isolated hStau polypeptides of the invention can be obtained in various ways, including by recombinant expression, purification from a biological sample or cell lysate, or chemical synthesis, and are free of components that naturally accompany it.

Recombinant hStau can be produced by ligating a nucleic acid sequence encoding hStau or a fragment thereof into a nucleic acid construct such as an expression vector, and transforming a bacterial or eukaryotic host cell with the expression vector. In general, nucleic acid constructs include a regulatory sequence operably linked to a hStau nucleic acid sequence. Regulatory sequences do not typically encode a gene product, but instead affect the expression of the nucleic acid sequence. In bacterial systems, a strain of Escherichia coli such as BL-21 can be used. Suitable E. coli vectors include without limitation the pGEX series of vectors that produce fusion proteins with glutathione S-transferase (GST). Transformed E. coli are typically grown exponentially, then stimulated with isopropylthiogalactopyranoside (IPTG) prior to harvesting. In general, such fusion proteins are soluble and can be purified easily from lysed cells by adsorption to glutathione-agarose beads followed by elution in the presence of free glutathione. The pGEX vectors are designed to include thrombin or factor Xa protease cleavage sites so that the cloned target gene product can be released from the GST moiety.

In eukaryotic host cells, a number of viral-based expression systems can be utilized to express hStau. A nucleic acid encoding hStau can be cloned into, for example, a baculoviral vector and then used to transfect insect cells. Alternatively, the nucleic acid encoding hStau can be introduced into a SV40, retroviral or vaccinia based viral vector and used to infect host cells.

Mammalian cell lines that stably express hStau can be produced by using expression vectors with the appropriate control elements and a selectable marker. For example, the eukaryotic expression vector pCDNA.3.1 + (Invitrogen, San Diego, Calif.) is suitable for expression of hStau in, for example, COS cells or HEK293 cells. Following introduction of the expression vector by electroporation, DEAE dextran, or other suitable method, stable cell lines can be selected. Alternatively, transiently transfected cell lines are used to produce hStau. Human Staufen also can be transcribed and translated in vitro using wheat germ extract or rabbit reticulocyte lysate.

Human Staufen polypeptide also can be purified from a biological sample or cell lysate using standard protein purification techniques such as affinity chromatography, gel-filtration, and ion-exchange chromatography. hStau activity can be assessed during purification by determining, for example, binding of RNA, and in particular, of hTR.

The invention also features isolated complexes of hStau and the catalytic subunit of telomerase. In reference to complexes, the term “isolated” refers to a complex that is free of cellular debris. Complexes of hStau and the catalytic subunit of telomerase can be isolated by methods described herein. For example, an antibody having specific binding affinity for hStau can be used to immunoprecipitate complexes of the invention. Alternatively, isolated complexes can be formed in vitro upon incubation of hStau and the catalytic subunit of telomerase. The catalytic subunit of telomerase (TERT) can be from yeast, human, or mouse. The DNA and amino acid sequences of the catalytic subunits from various organisms are available on GenBank.

Human Staufen Nucleic Acid Sequences

The invention also features isolated polynucleotides encoding a hStau polypeptide. As used herein, “isolated” refers to a sequence corresponding to part or all of the hStau gene, but free of sequences that normally flank one or both sides of the hStau gene in a mammalian genome. An isolated polynucleotide can be, for example, a recombinant DNA molecule, provided one of the nucleic acid sequences normally found immediately flanking that recombinant DNA molecule in a naturally-occurring genome is removed or absent. Thus, isolated polynucleotides include, without limitation, a recombinant DNA that exists as a separate molecule (e.g., a cDNA or genomic DNA fragment produced by PCR or restriction endonuclease treatment) independent of other sequences as well as recombinant DNA that is incorporated into a vector, an autonomously replicating plasmid, a virus (e.g., a retrovirus, adenovirus, or herpes virus), or into the genomic DNA of a prokaryote or eukaryote. In addition, an isolated polynucleotide can include a recombinant DNA molecule that is part of a hybrid or fusion polynucleotide.

It will be apparent to those of skill in the art that a polynucleotide existing among hundreds to millions of other polynucleotides within, for example, cDNA or genomic libraries, or gel slices containing a genomic DNA restriction digest is not to be considered an isolated polynucleotide.

“Polynucleotides” are at least about 14 nucleotides in length. For example, the polynucleotide can be about 14 to 20, 20-50, 50-100, or greater than 150 nucleotides in length. Polynucleotides can be DNA or RNA, linear or circular, and in sense or antisense orientation. For example, hStau polynucleotides can encode a polypeptide that includes the amino acid sequence of SEQ ID NO:2 or fragments thereof, or amino acid sequences substantially identical to such sequences. For example, the polynucleotide can encode a polypeptide having the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2.

Polynucleotides having a nucleotide sequence other than the nucleotide sequence of SEQ ID NO:1 also can encode hStau polypeptides. The degeneracy of the genetic code is well-known in the art; i.e., for many amino acids, there is more than one nucleotide triplet that serves as the codon for the amino acid. In addition, conservative amino acid substitutions can be introduced in the hStau polypeptide, as discussed above.

In certain embodiments, polynucleotides of the invention have at least 70% sequence identity to the nucleotide sequence of SEQ ID NO:1. The nucleic acid sequence can have, for example, at least 80%, 90%, or 95% sequence identity to SEQ ID NO:1. Generally, percent sequence identity is calculated by determining the number of matched positions in aligned nucleic acid sequences, dividing the number of matched positions by the total number of aligned nucleotides, and multiplying by 100. A matched position refers to a position in which identical nucleotides occur at the same position in aligned nucleic acid sequences. Nucleic acid sequences can be aligned by visual inspection, or by using sequence alignment software. For example, MEGALIGN® (DNASTAR, Madison, Wis., 1997) sequence alignment software, using default parameters for the Clustal algorithm, can be used to align polynucleotides. In this method, sequences are grouped into clusters by examining the distance between all pairs. Clusters are aligned as pairs, then as groups.

The invention also features polynucleotides that are at least 150 nucleotides in length and that hybridize under stringent conditions to the hStau polynucleotide of SEQ ID NO:1 or to the complement thereof. For example, the polynucleotide can include nucleotide 1 to about nucleotide 614 of SEQ ID NO:1, nucleotide 518 to nucleotide 3220 of SEQ ID NO:1, or nucleotides 1 to 3190 of SEQ ID NO:1. Hybridization typically involves Southern analysis (Southern blotting). See, for example, sections 9.37-9.52 of Sambrook et al., 1989 , “Molecular Cloning, A Laboratory Manual” , second edition, Cold Spring Harbor Press, Plainview; N.Y. Stringent conditions can include the use of low ionic strength and high temperature for washing, for example, 0.015 M NaCl/0.0015 M sodium citrate (0.1×SSC), 0.1% sodium dodecyl sulfate (SDS) at 60° C. Alternatively, denaturing agents such as formamide can be employed during hybridization, e.g., 50% formamide with 0.1% bovine serum albumin/0.1% Ficoll/0.1% polyvinylpyrrolidone/50 mM sodium phosphate buffer at pH 6.5 with 750 mM NaCl, 75 mM sodium citrate at 42° C. Another example is the use of 50% formamide, 5×SSC (0.75 M NaCl, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5×Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 0.1% SDS.

Human Staufen polynucleotides of the invention can be cloned from a cDNA library, such as a human testis cDNA library, or can be obtained by other means including chemical synthesis and polymerase chain reaction (PCR) technology using oligonucleotide pairs such as 5′-GCC TGG GAG GGG TGG TGG CCA TTT TTT G-3′ (SEQ ID NO:3) and 5′-GTT TGC TCT AGA ATG AAC GGT GGA AG-3′ (SEQ ID NO:4). PCR refers to a procedure or technique in which target nucleic acids are amplified. Generally, sequence information from the ends of the region of interest or beyond is employed to design oligonucleotide primers that are identical or similar in sequence to opposite strands of the template to be amplified. PCR can be used to amplify specific sequences from DNA as well as RNA, including sequences from total genomic DNA or total cellular RNA. Primers are typically 14 to 40 nucleotides in length, but can range from 10 nucleotides to hundreds of nucleotides in length. PCR is described, for example in PCR Primer: A Laboratory Manual , Ed. by Dieffenbach, C. and Dveksler, G., Cold Spring Harbor Laboratory Press, 1995. Nucleic acids also can be amplified by ligase chain reaction, strand displacement amplification, self-sustained sequence replication or nucleic acid sequence-based amplified. See, for example, Lewis, R. Genetic Engineering News , 12(9):1 (1992); Guatelli et al., Proc. Natl. Acad. Sci. USA , 87:1874-1878 (1990); and Weiss, R., Science, 254:1292 (1991).

Human Staufen Antibodies

Antibodies having specific binding affinity for human staufen polypeptide can be produced through standard methods. As used herein, the term “antibody” includes polyclonal antibodies, monoclonal antibodies, humanized or chimeric antibodies, single chain antibodies, Fab fragments, and F(ab) 2 fragments. In general, hStau can be produced in various ways, including recombinantly, or can be purified from a biological sample or cell lysate, and used to immunize animals.

Various host animals can be immunized by injection of an hStau polypeptide. Host animals include, for example, rabbits, chickens, mice, guinea pigs, and rats. Various adjuvants that can be used to increase the immunological response depend on the host species and include Freund's adjuvant (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin (KLH), and dinitrophenol. Polyclonal antibodies are heterogenous populations of antibody molecules that are contained in the sera of the immunized animals. Monoclonal antibodies, which are homogeneous populations of antibodies to a particular antigen, can be prepared using a hStau polypeptide and standard hybridoma technology. In particular, monoclonal antibodies can be obtained by any technique that provides for the production of antibody molecules by continuous cell lines in culture such as described by Kohler, G. et al., Nature, 256:495 (1975), the human B-cell hybridoma technique (Kosbor et al., Immunology Today, 4:72 (1983); Cole et al., Proc. Natl. Acad. Sci USA, 80:2026 (1983)), and the EBV-hybridoma technique (Cole et al., “Monoclonal Antibodies and Cancer Therapy”, Alan R. Liss, Inc., pp. 77-96 (1983)). Such antibodies can be of any immunoglobulin class including IgG, IgM, IgE, IgA, IgD, and any subclass thereof. The hybridoma producing the monoclonal antibodies of the invention can be cultivated in vitro and in vivo.

A chimeric antibody is a molecule in which different portions are derived from different animal species, such as those having a variable region derived from a murine monoclonal antibody and a human immunoglobulin constant region. Chimeric antibodies can be produced through standard techniques.

Single chain antibodies are formed by linking the heavy and light chain fragments of the Fv region via an amino acid bridge, resulting in a single chain polypeptide. Single chain antibodies can be produced through standard techniques. See, for example, U.S. Pat. No. 4,946,778.

Antibody fragments that have specific binding affinity for hStau polypeptide can be generated by known techniques. For example, such fragments include but are not limited to F(ab′) 2 fragments that can be produced by pepsin digestion of the antibody molecule, and Fab fragments that can be generated by reducing the disulfide bridges of F(ab′) 2 fragments. Alternatively, Fab expression libraries can be constructed. See, for example, Huse et al., Science, 246:1275 (1989). Once produced, antibodies or fragments thereof are tested for recognition of hStau by standard immunoassay methods including ELISA techniques, radioimmunoassays and Western blotting. See, Short Protocols in Molecular Biology , Chapter 11, Green Publishing Associates and John Wiley & Sons, Edited by Ausubel, F. M et al., 1992.

Methods for Inhibiting Telomerase Activity

The invention also provides methods for inhibiting telomerase activity. As described herein, ribosomal associated protein L22 and hStau both are RNA binding proteins that are associated with human telomerase complexes. Although both of these proteins are associated with hTR in vivo, they appear not to associate with each other. This suggests that hTR is present in multiple complexes in the cell and these distinct complexes may represent different stages of RNP assembly. RNA binding proteins may be critically involved in the maturation, localization, and assembly of the telomerase RNP. Thus, inhibition of the binding of the telomerase-associated proteins to the telomerase RNA may inhibit telomerase activity.

In general, telomerase activity can be inhibited by administering to a cell an amount of an agent effective to inhibit the interaction of a telomerase-associated polypeptide with a telomerase RNA. An agent can be a biological macromolecule such as an oligonucleotide or a polypeptide, a chemical compound, a mixture of chemical compounds, or an extract isolated from bacterial, plant, fungal, or animal matter.

A suitable polypeptide can be a telomerase-associated polypeptide. For example, an effective amount of a telomerase-associated polypeptide can bind the telomerase RNA such that the active telomerase core can not be formed. Thus, the polypeptide would titrate the telomerase RNA away from the catalytic component, and telomerase would be inhibited. The telomerase-associated polypeptide can include, for example, the RNA binding domains of hStau or L22. For example, a suitable hStau polypeptide can include RNA binding domains 1, 2, 3, or 4, or combinations thereof. RNA binding domains 1, 2, 3, and 4 of hStau include amino acids 59-79, 100-172, 202-275, and 452-472 of SEQ ID NO:2, respectively.

Suitable oligonucleotides can be RNA or DNA based nucleic acids including chimeric mixtures, derivatives, and modified versions thereof. The oligonucleotide can be modified at the base moiety, sugar moiety, or phosphate backbone, for example, to improve stability of the molecule or hybridization. A modified phosphate backbone can include, for example, phosphorothioate, phosphorodithioate, phosphoramidothioate, phosphoramidate, phosphordiamidate, methylphosphonate, alkyl phosphotriester, formacetel linkages, or analogs thereof. An oligonucleotide also can be a peptide nucleic acid, an uncharged nucleic acid derivative, which contains a pseudopeptide backbone. Peptide nucleic acids can be produced using standard techniques. See, for example, U.S. Pat. No. 5,539,082.

For example, an oligonucleotide can be an antisense oligonucleotide, i.e., complementary to at least a portion of a target RNA, to the coding sequence or transcribed untranslated region of a telomerase-associated polypeptide such as hStau or L22. Antisense oligonucleotides can be full-length or less than full-length. Antisense oligonucleotides that are less than full-length are typically at least 6 nucleotides in length, e.g., from 6 to about 200 nucleotides in length. The term “complementary” refers to a sequence that is able to hybridize with the RNA, forming a stable duplex under normal in vivo conditions. The ability to hybridize depends on both the degree of complementarily and the length of the antisense nucleic acid. Generally, the longer the hybridizing nucleic acid, the more base mismatches with an RNA it may contain and still form a stable duplex. One skilled in the art can ascertain a tolerable degree of mismatch by use of standard procedures to determine the melting point of the hybridized complex. Administration of an effective amount of such antisense oligonucleotides would prevent expression of telomerase-associated polypeptides, and inhibit telomerase activity.

Alternatively, an oligonucleotide can include an RNA binding site recognized by the telomerase-associated polypeptide. Administration of an effective amount of such an oligonucleotide provides the cell with an excess of the oligonucleotide. Consequently, telomerase-associated polypeptides bind the oligonucleotide rather than the telomerase RNA, and telomerase activity is inhibited. The binding specificity of L22 includes a stem loop structure with three conserved nucleotides at the top of the stem. Dobbelstein, M. et al., J. Virol., 1995, 69(12):8027-8034.

Oligonucleotides can be synthesized by standard methods known in the art, e.g., by use of an automated nucleic acid synthesizer (such as those commercially available from Biosearch, Applied Biosystems). Phosphorothioate oligonucleotides can be synthesized by the method of Stein et al., Nucl. Acids Res., 1988, 16:3209-3221. Methylphosphonate oligonucleotides can be prepared by use of controlled pore glass polymer supports, as described by Sarin et al., Proc. Natl. Acad. Sci. USA, 1988, 85(20):7448-7451.

In addition, ribozyme molecules can be designed to catalytically cleave telomerase-associated polypeptide transcripts, preventing expression of the telomerase-associated polypeptides. Various ribozymes that cleave RNA can be used. For example, hammerhead ribozymes cleave RNAs at locations dictated by flanking regions that form complementary base pairs with the target RNA. The sole requirement is that the target RNA have the following sequence of two bases: 5′-UG-3′. The construction and production of hammerhead ribozymes is known in the art. See, for example, U.S. Pat. No. 5,254,678. Alternatively, RNA endoribonucleases such as the one that occurs naturally in Tetrahymena thermophila can be used. See, for example, U.S. Pat. No. 4,987,071.

Oligonucleotides and ribozymes can be delivered to a cell in vivo by a number of methods. For example, oligonucleotides can be injected directly into the tissue site, e.g., a tumor, or can be administered systemically. Alternatively, recombinant DNA constructs can be used to express oligonucleotides, ribozymes, and telomerase-associated polypeptides of the invention. For example, a vector can be introduced in vivo such that it is taken up by a cell and directs the transcription of the oligonucleotide, ribozyme, or polypeptide. Vectors can remain episomal or can integrate into a chromosome, and are produced by standard recombinant DNA technology.

Inhibiting agents (other than antisense oligonucleotides) can be identified using an in vitro system. For example, telomerase activity can be reconstituted in vitro with telomerase RNA and TERT, the catalytic component of telomerase, in the presence of telomerase-associated polypeptides. See, Weinrich, R. L. et al., Nat. Genet., 1997, 17:498-502; and Beattie, T. L. et al., Curr. Biol., 1998, 8:177-180 for a description of reconstitution of telomerase. Various agents can be added to the reaction, and telomerase activity is monitored with standard procedures. See, Kim, N. W. and Wu, F., Nucl. Acids Res., 1997, 25:2595-2597 for a telomeric repeat amplification protocol (TRAP) for detecting telomerase activity.

A cell-based system also can be used to identify inhibiting agents. Agents to be screened can be administered to a cell, as discussed above, and telomerase activity then is monitored in the presence and absence of the agents. Cells suitable for screening express the telomerase-associated polypeptides and contain detectable telomerase activity.

The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims.

EXAMPLES

Example 1

Materials and methods

Identification of Telomerase RNA Binding Proteins

A three hybrid system was modified and used to screen for hTR binding proteins. Nucleotides 64-222 of hTR were fused to MS2 phage DNA to generate pLS112 plasmid, which also contains ura3. The sequence of hTR can be obtained under GenBank Accession No. U86046 S79400. To avoid transcriptional termination by the RNase P RNA polymerase III, four nucleotides in hTR-MS2 RNA were changed. Positions 82, 83, 102, and 103 were changed to A, A, A, C, respectively, to disrupt a string of uridines which was observed to cause transcriptional termination in preliminary experiments. The fusion RNA was detected on Northern blots, indicating it was transcribed and stable in yeast cells. The pLS112 plasmid was transformed into a modified yeast reporter strain (L40coatng) along with cDNA-GAD libraries from HeLa, Jurket, or human testes (provided by Dr. G. Hannon, Dr. L. VanAelst, and Clontech Inc., Palo Alto, Calif.). See, SenGupta, D. J. et al., Proc. Natl. Acad. Sci. USA, 1996, 93(16):8496-8501 for a description of yeast reporter strain L40. Positive clones that grew in the presence of 5 mM 3-aminotriazole (3 AT) and that stained positive for β-galactosidase were picked. These clones were tested for reporter gene activation in the absence of RNA plasmids, and only clones which activated in the presence of the hTR-MS2 RNA were characterized further. Positive clones from this screen were characterized further by DNA sequencing and expression in mammalian cells.

Cloning of hStau cDNA

Rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR) was used to clone hStau. Using a tagged human testis cDNA library (Marathon ready human testis cDNA from Clontech) and gene specific primers within the cloned region, both the 5′ and 3′ halves of the gene were amplified by PCR, according to manufacture protocols, using AP1 and hStau specific primers 10-9 5′-CAC CTC CAG CCT CTC TGG CAG GGG CTC-3′ (SEQ ID NO:5), and 10-10 5′-GGC AAA GGA AAG ACA AGA CAT GGC TGC G-3′ (SEQ ID NO:6). Each half was subcloned into pCRScript SK+ and sequenced. The 5′ fragment contained nucleotides 1 to about nucleotide 614 of SEQ ID NO:1. The 3′ fragment contained nucleotide 518 to about nucleotide 3220 of SEQ ID NO:1. The entire gene was then reconstructed by cloning both halves together into the pCRScript vector using an unique BamH1 restriction site. The full length gene was completely sequenced (SEQ ID NO:1). The first in frame methionine was preliminary assigned as the first amino acid in the protein, although there is no direct evidence for translation starting at this point. An AAUAA polyadenylation signal was found 1.3kb downstream of the stop codon (nucleotide 3172 of SEQ ID NO:1) for the longest predicted open reading frame. Using this assignment, the calculated molecular weight of the hStau protein was 55 kD, which agrees with the size of the protein identified on western blots (FIG. 2 ).

Production of Antibodies

Antibodies for L22 were generated using a synthetic peptide that corresponds with the N terminal region of the protein (CMAPVKKLVVKGG, SEQ ID NO:7). The peptide was coupled to KLH using standard procedures and antisera were raised in rabbits. To generate antibodies to hStau, the partial cDNA that was obtained in the initial three hybrid library was subcloned into the bacterial expression vector pGEX. This construct generated an N terminal fusion of GST with the C-terminal 448 amino acids of hStau (pLS 144A, i.e., residues 49 to 496 of SEQ ID NO:2). The fusion protein was overexpressed in bacteria, purified over a glutathione column using standard procedure (Pharmacia Biotech), and used to generate antibodies in rabbits (Covance Research Products Inc.)

Antisera from rabbits were initially screened for protein or peptide binding using an ELISA assay. Positive antisera then were tested for specificity on western blots and by immunoprecipitation. The antisera against L22 recognized a 15kD protein as expected in both cell lysates and in immunoprecipitations. Antibodies directed against hStau recognized an approximately 55kD protein in western blots on extracts and immunoprecipitations. This molecular weight is similar to the calculated molecular weight for the full length gene.

Northern and Western Analysis

Multi-tissue northern blots (Clontech) were hybridized with a 0.7 kb BamHI-EcoRI hStau cDNA probe according to the manufacturer's procedures. In particular, blots were hybridized at 68° C. in ExpressHyb solution (Clontech) for 1 hour with shaking. Blots were washed several times in 2×SSC, 0.05% SDS at room temperature, with a final wash in 0.1×SSC and 0.1% SDS at 60° C. for about 40 minutes. The blots then were re-probed with an actin probe without stripping the hStau signal. Signal intensity was quantified on a BAS 1500 PhosphorImager system (Fuji Medical System, Inc, Stamford, Conn.). Western analysis and immunoprecipitations (IP) were carried out according to Harlow, E. and D. Lane, Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1988. Human 293 cells were lysed using either NP40 (up to 1%) buffer or hypotonic buffer and used for western and IP analysis. Some hStau IP were done using hStau serum that was coupled to CNBr activated sepharose beads to avoid IgG bands having a similar molecular weight as hStau in the western analysis.

Reverse-transcriptase-PCR

RT-PCR was used to quantitate RNAs in the supernatant and pellet of IP reactions. RNAs were prepared from both supernatant and pellet fractions by phenol/chloroform extraction and ethanol precipitation. The same amount of total RNA was then used in first strand cDNA synthesis reactions using random hexamer primers and Superscript II reverse transcriptase (BRL). cDNAs then were PCR amplified using hTR, U2, U3, 7SL, or RNase P specific primers. The primers used are as follows: hTR, 5′-GCCTGGGAGGGGTGGTGGCCATTTTTTG-3′ (SEQ ID NO:3) and 5′-GTTTGCTCTAGAATGAACGGTGGAAG-3′ (SEQ ID NO:4); U2, 5′-ATCGCTTCTCGGCCTTTT-3′ (SEQ ID NO:8) and 5′-GGGTGCACCGTTCCTGGGA-3′ (SEQ ID NO:9); U3, 5′-GACTATACTTTCAGGGATCATTTC-3′ (SEQ ID NO:10) and 5′-CCACTCAGACCGCGTTCTCTC-3′ (SEQ ID NO:11); 7SL, 5′-GTGCCTGTAGTCCCAGCTAC-3′ (SEQ ID NO:12) and 5′-GAGACGGGGTCTCGCTATG-3′ (SEQ ID NO:13); RNase P, 5′-GGAAGGTCTGAGACTAG-3′ (SEQ ID NO:14) and 5′-ATCTCCTGCCCAGTCTG-3′(SEQ ID NO:15). Number of PCR cycles was adjusted for each amplification such that PCR amplification was in the linear range. Typically, 25 cycles were used to amplify hTR, whereas 18 cycles were used to amplify U2 and RNase P RNAs. For U3 and 7SL RNAs, 18 to 22 cycles generally were used. To control for genomic DNA contamination, cDNA synthesis reactions also were done in the absence of the reverse transcriptase. No signals were generated in the subsequent PCR reactions. PCR products were separated in 6% native polyacrylamide gel, dried and exposed. Signal intensities were quantified on a STORM Phosphorlmager system (Molecular Dynamics, Inc, Sunnyvale, Calif.).

hStau Copy Number Estimate

To estimate hStau protein copy number in 293 cells, serial dilutions of 293 total cell lysate and a recombinant fusion protein GST-hStauC (described above) were western blotted and probed with either crude antisera or affinity purified hStau antibody. The signal intensity from 293 extract was compared to that obtained from known amounts of recombinant protein and copy number was estimated accordingly. For example, intensity of the hStau band (55 kD) from 20 μg total protein from 293 extract was equivalent to that obtained on 10-20 ng of GST-hStauC (83 kD) recombinant proteins.

Telomerase assay

Cell extracts, immunoprecipitation supernatants, or pellet fractions were assayed in a two step telomerase assay (TRAP) similar to that previously described by Autexier, C. et al., EMBO J., 1996, 15(21):5928-5935. This two step procedure uses a limited number of PCR cycles for amplification of the telomerase products so that the signal will be in the linear range. Thus, relative signal intensities reflect relative activity in a semi quantitative manner. Negative controls were used that had either no extract or were RNase treated. An internal standard for product amplification in the PCR step of the assay also was included in each reaction.

Example 2

Identification of Telomerase-associated Proteins

To identify proteins that bind to the human telomerase RNA (hTR), a modified three-hybrid system was used. A region of the human telomerase RNA (nucleotide 64 to 222), which can form a potential stem loop structure, was fused to the MS2 phage RNA and expressed in yeast behind the RNaseP RNA polymerase III promoter. This region of hTR is required for activity in an in vitro reconstitution assay. Three different human cDNA-GAD fusion libraries were screened for proteins that interact with hTR. Several candidate were identified and the partial cDNAs clones were tagged with the HA epitope. These tagged clones were used to transfect 293 cells to test whether they would interact with hTR in human cells. Two proteins were identified which showed specific association with hTR after immunoprecipitation with HA antibody. The first protein was ribosomal associated protein L22, which also binds EBER (EBV encoded RNA) in EBV infected cells. The second protein was called telomerase-associated protein 3 (TEP3) or hStau (see below).

The full length cDNA encoding hStau was obtain from testis cDNA using a RACE technique. The hStau cDNA encodes a 496 amino acid open reading frame with a 1.3 kb long 3′ untranslated region. A motif search revealed several regions of the protein that contain homology to double stranded RNA binding domains that were originally identified in the Drosophila Staufen protein. The conserved double stranded RNA binding domains include both full length and short domains and are present in a diverse group of proteins including E. coli RNase III, Xenopus rbpa, human double stranded RNA activated kinase (DAI, also called protein kinase RNA binding protein or pKR), and the human TAR binding protein that binds HIV RNA. The hStau protein identified herein, like other double stranded RNA binding domain proteins, has two full length and two short domains. The organization of the domains in hStau is similar to that of Drosophila Staufen although hStau is shorter (FIG. 1 A). Furthermore, hStau sequence is most homologous to the Staufen protein, although this similarity is limited to the RNA binding domains (FIG. 1 B). This suggests hStau uses Staufen related motifs to bind to RNA.

Example 3

Interaction With hTR in vivo

To further examine the interaction of L22 and hStau with hTR in vivo, antibodies to both proteins were generated and used to test the ability of the antibodies to precipitate hTR. For L22, anti-peptide antibodies were made, while for hStau, antibodies to a recombinant GST fusion protein were generated as described in Example 1. Immunoprecipitation and western blotting showed that these antibodies were specific for the appropriate proteins (FIGS. 2 A and B). The L22 antibody precipitated a 15 kD protein that was not seen using pre-immune serum or when the peptide was pre-incubated with the antibody before the IP (FIG. 2 B). Anti-hStau antibody recognized a 55 kD protein on western blots of both cell lysates and immunoprecipitation reactions, consistent with its calculated molecular weight. (FIG. 2 A).

To examine whether L22 and hStau interact with hTR in vivo, immunoprecipitations were carried out on 293 cell lysates using antibodies against L22 and hStau, as well as several unrelated antibodies as controls. The amount of hTR was measured in the supernatant and pellet fraction of the immunoprecipitation reactions (FIG. 2 C). hTR was present in the pellets of the anti-hStau and anti-L22 reactions, but not in the pellets from the pre-immune sera or when anti-L22 antibody was pre-incubated with L22 peptides. To test for the specificity of the association, two unrelated antibodies, anti-GST and anti-chymotrypsin, were used. Telomerase RNA was not found in the pellet fraction of immunoprecipitation reactions with these antibodies. As a second control for specificity of the interaction with hTR, it was determined if four unrelated abundant small RNAs (U2, U3, 7SL RNA, and RNase P RNA) also were precipitated by the L22 and hStau antibodies (FIGS. 2 C and D). Most of these RNAs were not detected at significant levels in the immunoprecipitation pellets, although some U3 was detected in the pellet fraction of anti-hStau precipitation, it was not clear how specific this interaction was. The precipitation of U3 could be due to the high copy number of this RNA (2×10 5 per cell) compared to the hTR, which is only present at 500 copies per cell. These data suggest that hStau and L22 specifically associate with hTR in vivo.

Example 4

hStau and L22 Interact With Telomerase

To determine if hStau or L22 were associated with telomerase activity in vivo, telomerase activity was assayed in anti-hStau and anti-L22 immunoprecipitates. Immunoprecipitations were carried out using either anti-hStau, anti-L22, pre-immune serum, or an unrelated anti-GST antibody. Pellet fractions were washed with telomerase reaction buffer and a TRAP assay was performed. Telomerase activity was specifically immunoprecipitated with anti hStau antisera. Little activity was seen in immunoprecipitations with pre-immune sera and none with the anti-GST antibody ( FIG. 3 a ). Activity also was precipitated with anti-L22 antisera. Pre-incubation of the anti-L22 antisera with the L22 peptide significantly reduced the level of telomerase activity precipitated. Thus, both hStau and L22 are associated with telomerase activity in vivo.

Telomerase activity requires the catalytic subunit hTERT. To look directly at the association of hStau and L22 with hTERT, 293 cells were transfected with a cDNA that expresses hTERT tagged with the HA epitope. Cell extracts were made and antibodies directed against hStau and L22 were used in immunoprecipitation reactions. Western analysis of the pellet fraction was carried out using anti-HA antibodies. hTERT was precipitated by both anti-hStau and anti-L22 antisera, but not by pre-immune sera ( FIG. 3 b ). These results indicate that hStau and L22 protein associate with hTERT in vivo.

Example 5

Expression of hStau in Human Tissues

It has been demonstrated that L22 is widely expressed in cell lines. See, Toczyski, D. P. and J. A. Steitz, Mol. Cell. Biol., 1993, 13:703-710. Determination of the expression pattern of L22 was carried out by northern analysis. Under conditions of high stringency, the L22 probe identified three different transcripts in most human tissues suggesting there are either multiple spliced forms or multiple genes that encode this protein. Human Staufen expression was determined by both northern and western analyses. Northern analysis identified a 3.3 kb RNA that hybridized at high stringency to the hStau probe. This size was expected from the size of the cDNA clone. This RNA was expressed at similar levels in a wide variety of human tissues (FIGS. 4 A and 4 B), with a slightly higher level in testes. Thus, hStau is widely expressed in a variety of tissues.

L22 protein has been estimated to be present at approximately 107 copies per cell. To determine the level of hStau in cells, a dilution series of known amounts of recombinant GST-hStau fusion protein was compared with a dilution series of 293 cell extracts. From this data, it was estimated that there were approximate 106 copies of hStau per cell in the human 293 cell line.

The presence of a long untranslated 3′ region on the hStau cDNA suggested that it might be subject to translational regulation. To test whether the pattern of protein expression matched that of the RNA, western analysis was carried out on cell lysate from a variety of human tissues. The hStau protein was detected in protein lysates made from lung, kidney, testis and ovary, but not in brain and heart. Interestingly, the level was highest in the ovary and testes, which express telomerase in vivo. The discrepancy between the level of hStau protein in various tissues with the level of the RNA, suggests that hStau may be regulated by post transcriptional controls.

Example 6

Interactions Among Telomerase-associated Proteins

To test whether hStau or L22 interact with each other or with telomerase-associated protein 1 (TEP1), immunoprecipitation reactions were carried out. TEP1 is a homologue of the Tetrahymena p80 telomerase component, and interacts with hTERT in vivo. Harrington, L. et al., Science, 1997, 275:973-977. Antibodies directed against hStau immunoprecipitated hStau but not L22. Similarly, antibodies directed against L22 brought down L22 but not hStau (FIG. 5 A). These results suggested that hStau and L22 do not interact with each other. TEP1 was not detected in the immunoprecipitation reactions with either hStau or L22. The lack of a signal may have been, however, due to low affinity of the TEP1 antibody. For example, immunoprecipitation using TEP1 antibody brought down only very low levels of TEP1 and no hStau was detected.

To further examine the interaction of hStau and L22 with TEP1, 293 cells transfected with expression plasmids for myc-tagged TEP1 were used. In cells expressing myc-tagged TEP1, anti-hStau antibody immunoprecipitated hStau, but TEP1 was not detected by western analysis probing for the myc tag (FIG. 5 B). Similarly, anti-L22 antibody immunoprecipitated L22, but not myc-tagged TEP1 from myc-TEP1 expressed cells. (FIG. 5 C). However, myc-TEP1 protein did co-immunoprecipitate with overexpressed hTERT-HA, indicating that this tagged TEP1 protein is associated with telomerase. Thus, these results suggest that TEP1, hStau and L22 are not associated with each other, yet they are individually associated with hTERT.

The inability to detect interactions between TEP1 and either hStau or L22 might be due to the low abundance of the complexes that contain both proteins, or it may indicate that these proteins are present in multiple distinct complexes.

OTHER EMBODIMENTS

It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.

24 1 3190 DNA Homo sapiens 1ctagaattca gcggccgctg aattctaggg cggctgccgc gtccctctcg gctcccgctt 60cctttgaccg cctccccccc ccggcccggc ggcgcccgcc tcctccacgg ccactccgcc 120tcttccctcc cttcgtccct tcttcctctc ccttttttcc ttcttccttc ccctcctcgc 180cgccaccgcc caggaccgcc ggccggggga cgagctcgga gcagcagcca gagtttatta 240accacttaac ctctcagaac tgaacaaaga caacattgtt cctggaacgc cctcttttta 300aaaaagaaag cataacccct actgtagaac taaatgcact gtgcatgaaa cttggaaaaa 360aaccaatgta taagcctgtt gacccttact ctcggatgcg gtccacctat aactacaaca 420tgagaggagg tgcttatccc ccgaggtact tttacccatt tccagttcca cctttacttt 480atcaagtgga actttctgtg ggaggacagc aatttaatgg caaaggaaag acaagacagg 540ctgcgaaaca cgatgctgct gccaaagcgt tgaggatcct gcagaatgag cccctgccag 600agaggctgga ggtgaatgga agagaatccg aagaagaaaa tctcaataaa tctgaaataa 660gtcaagtgtt tgagattgca cttaaacgga acttgcctgt gaatttcgag gtggcccggg 720agagtggccc accccacatg aagaactttg tgaccaaggt ttcggttggg gagtttgtgg 780gggaaggtga agggaaaagc aagaagattt caaagaaaaa tgccgccata gctgttcttg 840aggagctgaa gaagttaccg cccctgcctg cagttgaacg agtaaagcct agaatcaaaa 900agaaaacaaa acccatagtc aagccacaga caagcccaga atatggccag gggatcaatc 960cgattagccg actggcccag atccagcagg caaaaaagga gaaggagcca gagtacacgc 1020tcctcacaga gcgaggcctc ccgcgccgca gggagtttgt gatgcaggtg aaggttggaa 1080accacactgc agaaggaacg ggcaccaaca agaaggtggc caagcgcaat gcagccgaga 1140acatgctgga gatccttggt ttcaaagtcc cgcaggcgca gcccaccaaa cccgcactca 1200agtcagagga gaagacaccc ataaagaaac caggggatgg aagaaaagta accttttttg 1260aacctggctc tggggatgaa aatgggacta gtaataaaga ggatgagttc aggatgcctt 1320atctaagtca tcagcagctg cctgctggaa ttcttcccat ggtgcccgag gtcgcccagg 1380ctgtaggagt tagtcaagga catcacacca aagattttac cagggcagct ccgaatcctg 1440ccaaggccac ggtaactgcc atgatagccc gagagttgtt gtatgggggc acctcgccca 1500cagccgagac cattttaaag aataacatct cttcaggcca cgtaccccat ggacctctca 1560cgagaccctc tgagcaactg gactatcttt ccagagtcca gggattccag gttgaataca 1620aagacttccc caaaaacaac aagaacgaat ttgtatctct tatcaattgc tcctctcagc 1680cacctctgat cagccatggt atcggcaagg atgtggagtc ctgccatgat atggctgcgc 1740tgaacatctt aaagttgctg tctgagttgg accaacaaag tacagagatg ccaagaacag 1800gaaacggacc aatgtctgtg tgtgggaggt gctgaacctt ttctggccat gaaccattat 1860aaaatcccaa catatatact gaaaatactg aaactgcttt gaaaatttgg aatttctgat 1920acctccagtg ggccgagaga cacggtgggt aaaggatgtg ggcagcagca gggaagacaa 1980cagaaacaca aggaggcggc tgtggccggg ctggactgtg ctggggtttg ttgtgatggc 2040cactcggtga cctggcggtc cctacgcaat agcagctgcc tgtggggaag aagggctgcc 2100cagccagctg gttctcccgg gacaccagca gatccacacc ctgggcacct ccgtgtttgg 2160tctttttttt cccctgtgtg aaagaagaaa cggcacgacc ccttctcaag ctggctcact 2220cagacacatt gggacaaacc ctggacagcc atgccagaga gaggcctttg accggcccca 2280gagctaaaag caccagagaa aatcaaatgc ttcctactca gcgtgaccca acttttctag 2340tgtgccacgg ccccaccacc tcctgcagta cccacaccat caccactgct ttctcttcca 2400acagtgatct gtattcttag tttcattatt ttcttttgat tgatatgaca ctatataaaa 2460ttttcatttg agaatttctc aattgtatct agttaaatag cacagtttgg aaacttgtct 2520gagactgact ttatcaataa tctaaccgac aaagatcata tccatgtgta tgtggttaga 2580catttttatt tcattgacta acccaggaca gtttcagtga tgcaaattgt gtgccctctg 2640gttcagctga aacagtcctg gactttcaaa aaccttgaat aagtctccca cagttgtata 2700aattggacaa tttaggaatt ttaaacttta gatgatcatt tggttccatt tttatttcat 2760ttttattttt gttaatgcaa acaggactta aatgaacttt gatctctgtt ttaaagatta 2820ttaaaaaaca ttgtgtatct atacatatgg ctcttgagga cttagctttc actacactac 2880aggatatgat ctccatgtag tccatataaa cctgcagagt gattttccag agtgctcgat 2940actgttaatt acatctccat tagggctgaa aagaatgacc tacgtttctg tatacagctg 3000tgttgctttt gatgttgtgt tactgtacac agaagtgtgt gcactgaggc tctgcgtgtg 3060gtccgtatgg aaagcctggt agccctgcga gttaagtact gcttccattc attgtttacg 3120ctggaatttt tctccccatg gaatgtaagt aaaacttaag tgtttgtcat caataaatgg 3180taatacttaa 3190 2 496 PRT Homo sapiens 2Met Lys Leu Gly Lys Lys Pro Met Tyr Lys Pro Val Asp Pro Tyr Ser 1 5 10 15Arg Met Arg Ser Thr Tyr Asn Tyr Asn Met Arg Gly Gly Ala Tyr Pro 20 25 30Pro Arg Tyr Phe Tyr Pro Phe Pro Val Pro Pro Leu Leu Tyr Gln Val 35 40 45Glu Leu Ser Val Gly Gly Gln Gln Phe Asn Gly Lys Gly Lys Thr Arg 50 55 60Gln Ala Ala Lys His Asp Ala Ala Ala Lys Ala Leu Arg Ile Leu Gln65 70 75 80Asn Glu Pro Leu Pro Glu Arg Leu Glu Val Asn Gly Arg Glu Ser Glu 85 90 95Glu Glu Asn Leu Asn Lys Ser Glu Ile Ser Gln Val Phe Glu Ile Ala 100 105 110Leu Lys Arg Asn Leu Pro Val Asn Phe Glu Val Ala Arg Glu Ser Gly 115 120 125Pro Pro His Met Lys Asn Phe Val Thr Lys Val Ser Val Gly Glu Phe 130 135 140Val Gly Glu Gly Glu Gly Lys Ser Lys Lys Ile Ser Lys Lys Asn Ala145 150 155 160Ala Ile Ala Val Leu Glu Glu Leu Lys Lys Leu Pro Pro Leu Pro Ala 165 170 175Val Glu Arg Val Lys Pro Arg Ile Lys Lys Lys Thr Lys Pro Ile Val 180 185 190Lys Pro Gln Thr Ser Pro Glu Tyr Gly Gln Gly Ile Asn Pro Ile Ser 195 200 205Arg Leu Ala Gln Ile Gln Gln Ala Lys Lys Glu Lys Glu Pro Glu Tyr 210 215 220Thr Leu Leu Thr Glu Arg Gly Leu Pro Arg Arg Arg Glu Phe Val Met225 230 235 240Gln Val Lys Val Gly Asn His Thr Ala Glu Gly Thr Gly Thr Asn Lys 245 250 255Lys Val Ala Lys Arg Asn Ala Ala Glu Asn Met Leu Glu Ile Leu Gly 260 265 270Phe Lys Val Pro Gln Ala Gln Pro Thr Lys Pro Ala Leu Lys Ser Glu 275 280 285Glu Lys Thr Pro Ile Lys Lys Pro Gly Asp Gly Arg Lys Val Thr Phe 290 295 300Phe Glu Pro Gly Ser Gly Asp Glu Asn Gly Thr Ser Asn Lys Glu Asp305 310 315 320Glu Phe Arg Met Pro Tyr Leu Ser His Gln Gln Leu Pro Ala Gly Ile 325 330 335Leu Pro Met Val Pro Glu Val Ala Gln Ala Val Gly Val Ser Gln Gly 340 345 350His His Thr Lys Asp Phe Thr Arg Ala Ala Pro Asn Pro Ala Lys Ala 355 360 365Thr Val Thr Ala Met Ile Ala Arg Glu Leu Leu Tyr Gly Gly Thr Ser 370 375 380Pro Thr Ala Glu Thr Ile Leu Lys Asn Asn Ile Ser Ser Gly His Val385 390 395 400Pro His Gly Pro Leu Thr Arg Pro Ser Glu Gln Leu Asp Tyr Leu Ser 405 410 415Arg Val Gln Gly Phe Gln Val Glu Tyr Lys Asp Phe Pro Lys Asn Asn 420 425 430Lys Asn Glu Phe Val Ser Leu Ile Asn Cys Ser Ser Gln Pro Pro Leu 435 440 445Ile Ser His Gly Ile Gly Lys Asp Val Glu Ser Cys His Asp Met Ala 450 455 460Ala Leu Asn Ile Leu Lys Leu Leu Ser Glu Leu Asp Gln Gln Ser Thr465 470 475 480Glu Met Pro Arg Thr Gly Asn Gly Pro Met Ser Val Cys Gly Arg Cys 485 490 495 3 28 DNA Artificial Sequence Primer 3gcctgggagg ggtggtggcc attttttg 28 4 26 DNA Artificial Sequence Primer 4gtttgctcta gaatgaacgg tggaag 26 5 27 DNA Artificial Sequence Primer 5cacctccagc ctctctggca ggggctc 27 6 28 DNA Artificial Sequence Primer 6ggcaaaggaa agacaagaca tggctgcg 28 7 13 PRT Artificial Sequence Peptide 7Cys Met Ala Pro Val Lys Lys Leu Val Val Lys Gly Gly 1 5 10 8 18 DNA Artificial Sequence Primer 8atcgcttctc ggcctttt 18 9 19 DNA Artificial Sequence Primer 9gggtgcaccg ttcctggga 19 10 24 DNA Artificial Sequence Primer 10gactatactt tcagggatca tttc 24 11 21 DNA Artificial Sequence Primer 11ccactcagac cgcgttctct c 21 12 20 DNA Artificial Sequence Primer 12gtgcctgtag tcccagctac 20 13 19 DNA Artificial Sequence Primer 13gagacggggt ctcgctatg 19 14 17 DNA Artificial Sequence Primer 14ggaaggtctg agactag 17 15 17 DNA Artificial Sequence Primer 15atctcctgcc cagtctg 17 16 73 PRT Homo sapiens BINDING (0)...(0) 16Leu Asn Lys Ser Glu Leu Ser Gln Val Phe Glu Ile Ala Leu Lys Arg 1 5 10 15Asn Leu Pro Val Asn Phe Glu Val Ala Arg Glu Ser Gly Pro Pro His 20 25 30Met Lys Asn Phe Val Thr Lys Val Ser Val Gly Glu Phe Val Gly Glu 35 40 45Gly Glu Gly Lys Ser Lys Lys Ile Ser Lys Lys Asn Ala Ala Ile Ala 50 55 60Val Leu Glu Glu Leu Lys Lys Leu Pro65 70 17 73 PRT Drosophila melanogaster BINDING (0)...(0) 17Lys Asp Lys Thr Pro Met Cys Leu Val Asn Glu Leu Ala Arg Tyr Asn 1 5 10 15Lys Ile Thr His Gln Tyr Arg Leu Thr Glu Glu Arg Gly Pro Ala His 20 25 30Cys Lys Thr Phe Thr Val Thr Leu Met Leu Gly Asp Glu Glu Tyr Ser 35 40 45Ala Asp Gly Phe Lys Ile Lys Lys Ala Gln His Leu Ala Ala Ser Lys 50 55 60Ala Ile Glu Glu Thr Met Tyr Lys His65 70 18 72 PRT Drosophila melanogaster BINDING (0)...(0) 18Asp Lys Lys Ser Pro Ile Ser Gln Val His Glu Ile Gly Ile Lys Arg 1 5 10 15Asn Met Thr Val His Phe Lys Leu Arg Glu Glu Gly Pro Ala His Met 20 25 30Lys Asn Phe Ile Thr Ala Cys Ile Val Gly Ser Ile Val Thr Glu Gly 35 40 45Glu Gly Asn Gly Lys Lys Val Ser Lys Lys Arg Ala Ala Glu Lys Met 50 55 60Leu Val Glu Leu Gln Lys Leu Pro65 70 19 74 PRT Homo sapiens BINDING (0)...(0) 19Gln Gly Ile Asn Pro Ile Ser Arg Leu Ala Gln Ile Gln Gln Ala Lys 1 5 10 15Lys Glu Lys Glu Pro Glu Tyr Thr Leu Leu Thr Glu Arg Cys Leu Pro 20 25 30Arg Arg Arg Glu Phe Val Met Gln Val Lys Val Gly Asn His Thr Ala 35 40 45Glu Gly Thr Cys Thr Asn Lys Lys Val Ala Lys Ala Asn Ala Ala Glu 50 55 60Asn Met Leu Glu Ile Leu Gly Phe Lys Val65 70 20 75 PRT Drosophila melanogaster BINDING (0)...(0) 20Asp Ala Asp Asn Pro Ile Thr Lys Leu Ile Gln Leu Gln Gln Thr Arg 1 5 10 15Lys Glu Lys Glu Pro Ile Phe Glu Leu Ile Ala Lys Asn Gly Asn Glu 20 25 30Thr Ala Arg Arg Arg Glu Phe Val Met Glu Val Ser Ala Ser Gly Ser 35 40 45Thr Ala Arg Gly Thr Gly Asn Ser Lys Lys Leu Ala Lys Arg Asn Ala 50 55 60Ala Gln Ala Leu Phe Glu Leu Leu Glu Ala Val65 70 75 21 21 PRT Homo sapiens BINDING (0)...(0) 21Gly Lys Gly Lys Thr Arg Gln Ala Ala Lys His Asp Ala Ala Ala Lys 1 5 10 15Ala Leu Arg Ile Leu 20 22 21 PRT Homo sapiens BINDING (0)...(0) 22Gly Ile Gly Lys Asp Val Glu Ser Cys His Asp Met Ala Ala Leu Asn 1 5 10 15Ile Leu Lys Leu Leu 20 23 21 PRT Drosophila melanogaster BINDING (0)...(0) 23Gly Ile Gly Arg Thr Leu Gln Gln Ala Lys His Asp Ala Ala Ala Arg 1 5 10 15Ala Leu Gln Val Leu 20 24 21 PRT Drosophila melanogaster BINDING (0)...(0) 24Gly Val Gly Lys Ser Ser Glu Glu Ser Gln Asn Asp Ala Ala Ser Asn 1 5 10 15Ala Leu Lys Ile Leu 20

Claims

1. An isolated polypeptide, wherein the polypeptide comprises the amino acid sequence of about residue 49 to about 496 of SEQ ID NO;2 or conservative subsitutions thereof.

2. The polypeptide of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2 or conservative substitutions thereof.

3. The polypeptide of claim 2, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2.

4. The polypeptide of claim 1, wherein the polypeptide comprises the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2.

5. The polypeptide of claim 1, wherein the polypeptide binds RNA.

6. The polypeptide of claim 5, wherein the polypeptide binds double-stranded RNA.

7. The polypeptide of claim 5, wherein the polypeptide binds human telomerase RNA.

8. The polypeptide of claim 3, wherein the polypeptide has a molecular weight of about 55 kDa.

9. An isolated complex, wherein the complex comprises a polypeptide complexed with the catalytic subunit of telomerase, wherein the polypeptide comprises the amino acid sequence of about residue 49 to about residue 496 SEQ ID NO:2 or conservative substitutions thereof.

10. An isolated polynucleotide encoding a human staufen polypeptide, wherein the encoded polypeptide comprises the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2 or conservative substitutions thereof.

11. The polynucleotide of claim 10, wherein the encoded polypeptide comprises the amino acid sequence of about residue 49 to about residue 496 of SEQ ID NO:2 of conservative substitutions thereof.

12. The polynucleodide of claim 10, wherein the encoded polypeptide comprises the amino acid sequence of SEQ ID NO:2 or conservative substitutions thereof.

13. The polynucleotide of claim 10, wherein the polynucleotide has a nucleotide sequence at least 70% identical to the nucleotide sequence of nucleotides 345 to 1835 of SEQ ID NO:1.

14. The polynucleotide of claim 10, wherein the polynucleotide has a nucleotide sequence at least 80% identical to the nucleotide sequence of nucleotides 345 to 1835 of SEQ ID NO:1.

15. The polynucleotide of claim 10, wherein the polynucleotide has a nucleotide sequence at least 90% identical to the nucleotide sequence of nucleotides 345 to 1835 of SEQ ID NO:1.

16. The polynucleotide of claim 10, wherein the polynucleotide has a nucleotide sequence at least 95% identical to the nucleotide sequence of nucleotides 345 to 1835 of SEQ ID NO:1.

17. The polynucleotide of claim 10, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:1.

18. An expression vector comprising the polynucleotide of claim 10 operably linked to a regulatory sequence.

19. A cultured cell transfected with the vector of claim 18, wherein said cell expresses said polypeptide.

20. A method of producing a polypeptide, comprising culturing said cell of claim 19 under conditions permitting expression of said polypeptide.

21. A purified antibody having specific binding affinity for a human staufen polypeptide.

22. The antibody of claim 21, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:2.

23. The antibody of claim 21, wherein the antibody is polyclonal.

24. The antibody of claim 21, wherein the antibody is monoclonal.