Claims
- 1. A test carrier useful in determining a specific ion in a liquid sample comprising:
- a) a first test layer having a liquid resistant porous film layer having an organic phase which contains a homogeneous mixture of
- (i) a hydrophobic polymer,
- (ii) a plasticizer,
- (iii) an ionophore,
- (iv) a substance which changes color in the presence of the ion to be determined, and
- (v) a plurality of particles having an oil absorption value from 80-200, wherein said ionophore is homogeneously dispersed in said organic phase, and
- b) a second test layer containing a buffer substance capable of maintaining the pH in said second test layer at a value of between 5-10 wherein said first and second layers are positioned in said test carrier to permit fluid contact therebetween.
- 2. Test carrier of claim 1, wherein said hydrophobic polymer is a copolymer of vinyl acetate.
- 3. Test carrier of claim 1 comprising porous particles.
- 4. Test carrier of claim 3, wherein said hydrophobic polymer and said porous particles are present in a weight ratio ranging from about 5:1 to about 1:10.
- 5. Test carrier of claim 4, wherein said weight ratio ranges from 1:1 to about 1:3.
- 6. Test carrier of claim 1, wherein said plasticizer is selected from the group consisting of a sebacic acid ester, a phthalic acid ester, an acrylic acid ester, a phosphoric acid ester and a silicon.
- 7. Test carrier of claim 1, wherein said hydrophobic polymer and said plasticizer are present in a weight ratio ranging from about 5:1 to about 1.5.
- 8. Test carrier of claim 7, wherein said weight ratio is from about 2:1 to 1:2.
- 9. Test carrier of claim 1, wherein said substance which changes color is of the formula ##STR7## wherein R.sup.1, R.sup.2 and R.sup.3 are the same or different and are selected from the group consisting of hydrogen, an alkyl group and a alkoxy group, wherein at least one of R.sup.1, R.sup.2 and R.sup.3 is an alkyl or alkoxy group having from 8 to 30 carbons,
- R.sup.4 is hydrogen or an alkyl group,
- R.sup.5 is a nitro group, an alkyl group substituted by halogen, a cyano group, a sulfonamide group or an alkyl sulfonyl group,
- X is nitrogen or CR.sup.6, and
- Y is sulphur or CR.sup.7 .dbd.CR.sup.8
- wherein R.sup.6, R.sup.7 and R.sup.8 are the same or different and are selected from the group consisting of hydrogen, halogen, a nitro group, a cyano group, an unsubstituted alkyl group, an alkyl group substituted by halogen and an alkyl sulfonyl group.
- 10. Test carrier of claim 1 wherein said first test layer further comprises an alkyl phenol having at least two nitro groups, alkoxynaphthol having at least two nitro groups or a carbonyldicyanidophenyl hydrazone.
- 11. Test carrier of claim 1 wherein said buffer substance is a Good buffer.
- 12. Method for determining a specific ion in a liquid sample comprising contacting said liquid sample to the test carrier of claim 1 and determining a change in color of said substance which changes color in the presence of said specific ion.
- 13. Method of claim 12, comprising determining said change in color about 60 seconds after contacting the liquid sample thereto.
- 14. The test carrier of claim 1 wherein said buffer substance is selected from the group consisting of N-N-bis-(hydroxyethyl)-aminoethansulphonic acid 3-methylamino-hydroxypropane-sulphonic acid and N-hydroxyethylpiperazine-N-propanesulphonic acid.
- 15. The test carrier of claim 1 wherein the plasticizer is 2,2-diphenyl-1-cyano-acrylic acid-ethylhexylester.
- 16. The test carrier of claim 1 wherein said plasticizer is selected from the group consisting of a sebacic acid ester, a phthalic acid ester, an acrylic acid ester, a phosphoric acid ester and a silicon.
- 17. The test carrier of claim 1 wherein said hydrophobic polymer and said plasticizer are present in a weight ratio ranging from about 5:1 to above 1:5.
- 18. The test carrier of claim 17 wherein said weight ratio is from about 2:1 to about 1:2.
- 19. The test carrier of claim 1 wherein said hydrophobic polymer is a copolymer of vinylacetate with vinyl laurate.
- 20. The test carrier of claim 1 wherein said second test layer further contains a thickening agent.
- 21. Test carrier of claim 1, wherein said substance which changes color is a pH indicator.
- 22. Test carrier of claim 1, wherein said substance which changes color is 4-(2-bromo-4-nitro-6-trifluoromethylphenylazo-)-2-octadecyloxy-naphtol-1.
- 23. Test carrier of claim 1, wherein said ionophore is selected from the group consisting of 2,3-naphthol-15-crown-5, valinomycin, N,N'-dibenzyl-N,N-diphenyl-1,2-phenylenedioxydiacetamide, N,N'-diheptyl-5,5-dimethyl-N,N'-di(3-oxapentyl)-3,7-dioxanonane-diamide and diethyl-N-N'-bis-(12-methylaminododecanoate).
Priority Claims (1)
Number |
Date |
Country |
Kind |
4015590 |
May 1990 |
DEX |
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Parent Case Info
This is a divisional application of application Ser. No. 698,466, filed May 10, 1991, now U.S. Pat. No. 5,211,914.
US Referenced Citations (7)
Foreign Referenced Citations (5)
Number |
Date |
Country |
0016387 |
Oct 1980 |
EPX |
0125554 |
Nov 1984 |
EPX |
0153641 |
Sep 1985 |
EPX |
0175990 |
Apr 1986 |
EPX |
0207392 |
Jan 1987 |
EPX |
Non-Patent Literature Citations (2)
Entry |
Kirk-Othmer Encyclopedia of Chemical Technology (1982) Third Edition, vol. 17, pp. 800-801. |
Kirk-Othmer Encyclopedia of Chemical Technology (1982) Third Edition, vol. 10, pp. 210-213. |
Divisions (1)
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Number |
Date |
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Parent |
698466 |
May 1991 |
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