Claims
- 1. An isolated polypeptide comprising an amino acid sequence that is at least 70% identical to a reference amino acid sequence selected from the group consisting of (a) amino acid residues 75 to 187 of SEQ ID NO:2, (b) amino acid residues 281 to 604 of SEQ ID NO:2, and (c) amino acid residues 1 to 655 of SEQ ID NO:2, wherein the isolated polypeptide specifically binds with an antibody that specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:2.
- 2. The isolated polypeptide of claim 1, wherein the isolated polypeptide comprises an amino acid sequence that is at least 80% identical to a reference amino acid sequence.
- 3. The isolated polypeptide of claim 1, wherein the isolated polypeptide comprises an amino acid sequence that is at least 90% identical to a reference amino acid sequence.
- 4. The isolated polypeptide of claim 1, wherein the polypeptide comprises an amino acid motif having the sequence QLEQL[R, S, N][S, A]MGF[L, I]NREANLQALIATGGD[V, I][D, N]AA, wherein the sequence is further defined by at least one condition selected from the group consisting of: (a) the sixth residue of the motif is R, (b) the twenty-seventh residue is V, (c) the twenty-eighth residue is D, and (d) the seven residue is S when the eleventh residue is L.
- 5. The isolated polypeptide of claim 1, comprising an amino acid sequence consisting of amino acid residues 1 to 655 of SEQ ID NO:2.
- 6. The isolated polypeptide of claim 1, consisting of the amino acid sequence of SEQ ID NO:2.
- 7. An isolated nucleic acid molecule, wherein the nucleic acid molecule is either (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:3, or (b) a nucleic acid molecule that remains hybridized following stringent wash conditions to a nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:1, or the complement of SEQ ID NO:1.
- 8. The isolated nucleic acid molecule of claim 7, wherein any difference between the amino acid sequence encoded by the nucleic acid molecule and the corresponding amino acid sequence of SEQ ID NO:2 is due to a conservative amino acid substitution.
- 9. The isolated nucleic acid molecule of claim 7, comprising the nucleotide sequence of nucleotides 86 to 2050 of SEQ ID NO:1.
- 10. A vector, comprising the isolated nucleic acid molecule of claim 9.
- 11. An expression vector, comprising the isolated nucleic acid molecule of claim 9, a transcription promoter, and a transcription terminator, wherein the promoter is operably linked with the nucleic acid molecule, and wherein the nucleic acid molecule is operably linked with the transcription terminator.
- 12. A recombinant host cell comprising the expression vector of claim 11, wherein the host cell is selected from the group consisting of bacterium, yeast cell, fungal cell, insect cell, avian cell, mammalian cell, and plant cell.
- 13. A method of producing Zalpha16 protein, the method comprising culturing recombinant host cells that comprise the expression vector of claim 11, and that produce the Zalpha16 protein.
- 14. The method of claim 13, further comprising the step of isolating the Zalpha16 protein from the cultured recombinant host cells.
- 15. An antibody or antibody fragment that specifically binds with the polypeptide of claim 1.
- 16. An anti-idiotype antibody that specifically the binds the antibody of claim 15.
- 17. A method of detecting the presence of Zalpha16 RNA in a biological sample, comprising:
(a) contacting a Zalpha16 nucleic acid probe under hybridizing conditions with either (i) test RNA molecules isolated from the biological sample, or (ii) nucleic acid molecules synthesized from the isolated RNA molecules, wherein the probe consists of a nucleotide sequence comprising a portion of the nucleotide sequence of the nucleic acid molecule of claim 9, or complements thereof, and (b) detecting the formation of hybrids of the nucleic acid probe and either the test RNA molecules or the synthesized nucleic acid molecules, wherein the presence of the hybrids indicates the presence of Zalpha16 RNA in the biological sample.
- 18. A method of detecting the presence of Zalpha16 in a biological sample, comprising:
(a) contacting the biological sample with an antibody, or an antibody fragment, of claim 15, wherein the contacting is performed under conditions that allow the binding of the antibody or antibody fragment to the biological sample, and (b) detecting any of the bound antibody or bound antibody fragment.
- 19. A composition, comprising a carrier and the polypeptide of claim 6.
- 20. A fusion protein, comprising the polypeptide of claim 6.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional application No. 60/128,210 (filed Apr. 7, 1999), No. 60/166,040 (filed Nov. 17, 1999), and Ser. No. 09/541,919 (filed Apr. 3, 2000) the contents of which are incorporated by reference.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60166040 |
Nov 1999 |
US |
|
60128210 |
Apr 1999 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09541919 |
Apr 2000 |
US |
Child |
10001632 |
Oct 2001 |
US |