Patent Application
20080038220
The present invention relates to testosterone-containing pharmaceutical compositions and gels, and to methods using the same.
Testosterone in Men
Testosterone, the major circulating androgen in men, is synthesized from cholesterol. The approximately 500 million Leydig cells in the testes secrete more than 95% of the 6-7 mg of testosterone produced per day. Two hormones produced by the pituitary gland, luteinizing hormone (“LH”) and follicle stimulating hormone (“FSH”), are required for the development and maintenance of testicular function and negatively regulate testosterone production. Circulating testosterone is metabolized to various 17-keto steroids through two different pathways. Testosterone can be metabolized to dihydrotestosterone (“DHT”) by the enzyme 5-alpha-reductase or to estradiol (“E2”) by an aromatase enzyme complex. Testosterone circulates in the blood 98% bound to protein. In men, approximately 40% of the binding is to the high-affinity sex hormone binding globulin (“SHBG”). The remaining 60% is bound weakly to albumin. Thus, a number of measurements for testosterone are available from clinical laboratories.
The following table from the UCLA-Harbor Medical Center summarizes the hormone concentrations in normal adult men:
There is considerable variation in the half-life of testosterone reported in the literature, ranging from 10 to 100 minutes. Researchers do agree, however, that circulating testosterone has a diurnal variation in normal young men. Maximum levels occur at approximately 6:00 to 8:00 a.m. with levels declining throughout the day. Characteristic profiles have a maximum testosterone level of 720 ng/dL and a minimum level of 430 ng/dL. The physiological significance of this diurnal cycle, if any, however, is not clear.
Testosterone in Women
The excretion of androgenic steroids in the urine of adult women was demonstrated more than 50 years ago. Since that time, physiologists and clinicians have explored the sources and biological functions of testosterone and other endogenous androgenic hormones in the human female. It is now known that androgens are secreted by both the ovaries and adrenal glands in women. Each source contributes about 50% (directly and through precursors) to the approximately 300 μg of testosterone produced daily in healthy “cycling” women. While the adverse effects of excess androgen production, as occurs in the polycystic ovary syndrome and certain androgen producing tumors, have been well described, the normal physiological effects of androgens in women have been much less appreciated. As inferred from animal studies, male physiology, and the symptoms of women with deficient androgen production, the major physiological effects of androgens in normal women include, but are not limited to anabolic effects on muscle, skin, hair and bone; stimulatory effects on erythropoiesis; modulatory effects on immune function; and psychological effects on mood, well-being and sexual function.
In addition, endogenous androgens are important for the development of pubic hair and are thought to modulate the action of estrogens and progestins on a variety of reproductive target tissues. It is also believed that androgens play an important role in modulating the secretory function of the lacrimal gland.
Fifty percent of circulating testosterone is derived from direct ovarian secretion in the thecal cells under the control of luteinizing hormone. The other half is derived from peripheral conversion of adrenal androgen precursors dehydroepiandrosterone, androstenedione, and dehydroepiandrosterone sulphate. Testosterone can also be converted to dihydrotestosterone or estradiol. Thus, testosterone serves as both a hormone and as a pro-hormone.
Testosterone circulates in the blood 98% bound to protein. In women, approximately 66% of the binding is to the high-affinity sex hormone binding globulin. The remaining 34% is bound weakly to albumin. Thus, a number of measurements for testosterone are available from clinical laboratories. The order of affinity for the steroids most strongly bound by sex hormone binding globulin is dihydrotestosterone>testosterone>androstenedione>estradiol>estrone. Sex hormone binding globulin weakly binds dihydrotestosterone, but not dihydrotestosterone sulfate. The table below shows the approximate hormonal levels in normal pre-menopausal women.
In comparison to other hormone deficiency states, testosterone deficiency in women has been largely ignored as a clinical entity. Nevertheless, there exist well-defined subject populations where androgen production is clearly deficient and where associated symptomatology has been described, including, for example, young oophorectomized/hysterectomized women, post-menopausal women on estrogen replacement therapy, women on oral contraceptives, women with adrenal dysfunction, women with corticosteroid-induced adrenal suppression, and human immunodeficiency virus-positive women.
Because increasing testosterone concentrations has been shown to alter sexual performance and libido, researchers have investigated methods of delivering testosterone to men, and also to women. These methods include intramuscular injections, oral replacement, pellet implants, and transdermal patches.
These testosterone delivery methods, however, suffer from one or more drawbacks. For example, sub-dermal pellet implants and ester injections are painful and require surgical procedure and/or doctor visits. Moreover, in hypogonadal men implant therapy includes a risk of extrusion (8.5%), bleeding (2.3%), or infection (0.6%). Many of these methods, such as oral/sublingual/buccal preparations, suffer from undesirable pharmacokinetic profile-creating supra-physiologic testosterone concentrations followed a return to baseline. Transdermal patches provide less than optimal pharmacokinetic characteristics, are embarrassing for many subjects, and are associated with significant skin irritation. In addition, patches do not provide dosing flexibility, might be visually unappealing and may have a tendency to fall off, especially during rigorous physical exercise. Oral administration produces inappropriate testosterone levels and unpredictable absorption patterns between subjects. Moreover, because the liver metabolizes the preparation, there is a risk of hepatotoxicity not to mention first pass metabolism.
Recently, a one-percent testosterone gel has been approved for use in men, and provides dosing flexibility with minimal skin irritation. This gel is available in the United States under the trademark AndroGel® from Unimed Pharmaceuticals, Inc., Deerfield, Ill., a SolvayPharmaceuticals, Inc. Co., Marietta, Ga. 30062. Composition of AndroGel®:
*corresponding to 67 g of absolute ethanol.
The present invention provides testosterone-containing pharmaceutical compositions and gels, and methods for using the same. More particularly, the present invention provides testosterone-containing hydroalcoholic/alcoholic pharmaceutical compositions and gels, suitable for topical and transdermal application, wherein said compositions contain propylene glycol. In addition to achieving a very effective testosterone delivery, the compositions of the invention are aesthetically very attractive. Propylene glycol was shown to be a very potent penetration enhancer in the compositions of the invention, used as sole penetration enhancer or in combination with further penetration enhancer(s), e.g. with isopropyl myristate.
The present invention relates to a pharmaceutical composition comprising testosterone, at least one C2-C6 alcohol, at least one gelling agent, at least one penetration enhancer, and water.
Said pharmaceutical composition can be made in various forms, e.g. a gel, a solution, a cream, a lotion, a spray, an ointment, an aerosol. Preferably, said pharmaceutical composition is a gel.
The term “testosterone” as used hereafter refers not only to testosterone itself, but also to its enantiomers, isomers, tautomers, salts, chelates, esters, amides, derivatives, and pro-drugs and precursors thereof, e.g. DHT (dihydrotestosterone), 17-methyltestosterone, 17[alpha]-methyl-testosterone 3-cyclopentyl enol ether, testosterone enanthate, testosterone cypionate, testosterone undecanoate, testosterone cyclodextrin, testosterone buciclate. According to the invention, testosterone can be of natural origin, or result from a hemisynthesis or synthesis process.
C2-C6 alcohols are known in the art. Such alcohols encompass ethanol, propanol, isopropanol (propan-2-ol), n-propanol (propan-1-ol), butanol, butan-1-ol, butan-2-ol, ter-butanol, pentanols, hexanols. Ethanol is preferred, since it contributes efficiently towards the transdermal passage of testosterone by evaporating quickly on contact with the skin.
Gelling agents are known in the art. The term ‘gelling agent’ generally refers to a compound, possibly of polymeric nature, having the capacity to gel when contacted with a specific solvent, e.g. water. Gelling agents make it possible to increase the viscosity of the pharmaceutical compositions according to the invention, but may also act as solubilizing agents. Examples of gelling agents include anionic polymers such as acrylic acid based polymers (including polyacrylic acid polymers, e.g. CARBOPOL® by B.F. Goodrich Specialty Polymers and Chemicals Division of Cleveland, Ohio)., cellulose derivatives, poloxamers and poloxamines, more precisely, Carbomers or acrylic acid-based polymers, e.g. Carbopol® 980 or 940, 981 or 941, 1382 or 1382, 5984, 2984, 934 or 934P (Carbopol® are usually polymers of acrylic acid crosslinked with allyl sucrose or allylpentaerythritol), Pemulen TR1® or TR2®, Ultrez, Synthalen CR, etc.); cellulose derivatives such as ethylcelluloses, hydroxypropylcelluloses, hydroxyethylcelluloses, hydroxypropylmethylcelluloses (HPMC), carboxymethylcelluloses (CMC), etc.; poloxamers or polyethylene-polypropylene copolymers such as Lutrol® grade 68 or 127, poloxamines and other gelling agents such as chitosan, dextran, pectins, and natural gums. All of these gelling agents, alone or in combination, may be used in the pharmaceutical composition according to the invention. Said gelling agent can be selected taking into account the pH of the composition according to the invention and the desired viscosity.
Hydroxypropylcellulose, Carbopol® 980 and Lutrol® are particularly preferred in the context of the present invention.
Penetration enhancers are also known in the art. A ‘penetration enhancer’ is generally an agent known to accelerate the delivery of the drug or active principle through the skin. These agents also have been referred to as penetration accelerants, adjuvants, and absorption promoters. This class of agents includes those with diverse mechanisms of action including those which have the function of improving the solubility and diffusibility of the drug, and those which improve transdermal absorption by changing the ability of the stratum corneum to retain moisture, softening the skin, improving the skin's permeability, acting as penetration assistants or hair-follicle openers or changing temporarily the state of the skin such as the boundary layer.
Unless otherwise stated, percentages (%) refer to amounts by weight based upon total weight of the composition.
According to one aspect, said pharmaceutical composition comprises 0.5 to 5.0% (w/w), preferably 0.5 to 2.5% (w/w), more preferably 0.6 to 2.0% (w/w), even more preferably 0.7 to 1.5% (w/w), even more preferably 0.75 to 1.25% (w/w), even more preferably 0.8 to 1.2% (w/w), and even more preferably 0.9 to 1.1% (w/w), most preferably about 1.0% (w/w) of testosterone.
According to another aspect of the invention, said pharmaceutical composition comprises 0.5 to 5.0% (w/w), preferably 0.5 to 2.5% (w/w), more preferably 0.75 to 2.25% (w/w), even more preferably 0.9 to 2.0% (w/w), even more preferably 1.0 to 1.8% (w/w), even more preferably 1.25 to 1.75% (w/w), still more preferably 1.3 to 1.6% (w/w), most preferably about 1.5% (w/w) of testosterone.
According to another aspect, said pharmaceutical composition comprises 40.0 to 75.0% (w/w), preferably, 40.0 to 70.0% (w/w), more preferably 45.0 to 65.0% (w/w), even more preferably 50.0 to 60.0% (w/w), even more preferably 52.0 to 58.0% (w/w), even more preferably 53.0 to 57.0% (w/w), still more preferably 54.0 to 56.0% (w/w), most preferably about 56% (w/w) of at least one C2-C6 alcohol.
According to another aspect of the invention, said C2-C6 alcohol is selected from the group consisting of ethanol, propan-1-ol and propan-2-ol, and mixtures thereof. Preferably said C2-C6 alcohol is ethanol.
According to another aspect of the invention, said pharmaceutical composition comprises 0.1 to 5.0% (w/w), preferably 0.15 to 4.5% (w/w), more preferably 0.2 to 4.0% (w/w), even more preferably 0.25 to 3.5% (w/w), even more preferably 0.3 to 3.0% (w/w), even more preferably 0.4 to 2.5% (w/w), still more preferably 0.5 to 2.0% (w/w), most preferably about 0.5 to 1.0% (w/w) of at least one gelling agent. According to another aspect of the invention, said gelling agent is selected from the group consisting of acrylic acid-based polymers, including cellulosics, including cellulose esters and derivatives (cellulose derivatives such as ethylcellulose, hydroxypropylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose (HPMC), carboxymethylcellulose (CMC), etc); Carbomers such as Carbopol® polymers, e.g. Carbopol® 980 or 940, 981 or 941, 1382 or 1382, 5984, 2984, 934, or 934P, Pemulen TR1® or TR2®, Ultrez, Synthalen CR, etc.; poloxamines, poloxamers or polyethylene-polypropylene copolymers such as Lutrol® grade 68 or 127, poloxamines or other gelling agents such as chitosan, dextran, pectins, and natural gums, and mixtures thereof.
According to another aspect of the invention, said pharmaceutical composition comprises 0.1 to 5.0% (w/w), preferably 0.15 to 4.5% (w/w), more preferably 0.2 to 4.0% (w/w), even more preferably 0.3 to 3.5% (w/w), even more preferably 0.4 to 3.0% (w/w), still more preferably 0.5 to 2.5% (w/w), most preferably 0.5 to 2.0% (w/w) of propylene glycol. Advantageously according to the invention, for the testosterone contents and compositions of the invention, propylene glycol was found to be a very effective and compatible penetration enhancer. In addition, the use of propylene glycol according to the invention leads to pharmaceutical compositions having a very attractive appearance. The pharmaceutical compositions of the invention are very transparent, visually attractive, and have very pleasant texture, which improves the use by a patient. In addition, the pharmaceutical compositions of the invention require lower alcohol contents. Moreover, the compositions of the invention are very stable, and propylene glycol has proven to be compatible with standard neutralizers, e.g. triethanolamine. The compositions according to the invention may include several penetration enhancers.
According to the present invention, said pharmaceutical composition optionally further comprises water.
In one embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
Said pharmaceutical composition is suitable for the controlled transdermal delivery of testosterone. Thus, said pharmaceutical composition can be auto-administered by the patient him/herself, and does not require the presence of a physician during administration. Also, the administration does not require surgery or injections.
Advantageously according to the invention, propylene glycol acts as a very potent penetration enhancer for testosterone, in particular in combination with the testosterone dosage and the gelling agent(s) of the present composition.
In another aspect, the pharmaceutical composition of the invention further comprises a base. Advantageously, said base is pharmaceutically acceptable, and is preferably selected from the group consisting of triethanolamine, sodium hydroxide, ammonium hydroxide, potassium hydroxide, arginine, aminomethylpropanol or tromethamine, and mixtures thereof. Where the pH of said pharmaceutical composition is not optimized for transdermal administration, e.g. where said gelling agent comprises at least one acrylic acid-based polymer, said base contributes to the neutralization of said pharmaceutical composition, for topical application onto human skin. Furthermore, said base (neutralizer) allows optimum swelling of the polymer chains during the neutralization of the charges and the formation of polymer salts. Especially when said gelling agent comprises an acrylic acid-based polymer, said base preferably comprises triethanolamine. It also allows an optimum viscosity to be achieved in the pharmaceutical composition according to the invention. The skilled person would know how to choose a suitable amount of said base in the composition, especially with respect to the nature of said gelling agent present therein, and the alcohol content of the composition, in order to reach the desired final pH in the composition. For example, with carbomers and/or if there is a high alcohol content, one can use tromethamine and/or NaOH as a base, in amounts chosen as to reach the desired final pH in the composition. Alternatively, in one embodiment, the pharmaceutical composition according to the invention comprises 0.1 to 5.0% (w/w), preferably 0.15 to 4.5% (w/w), more preferably 0.2 to 4.0% (w/w), even more preferably 0.25 to 3.5% (w/w), even more preferably 0.3 to 3.0% (w/w), even more preferably 0.4 to 2.5% (w/w), still more preferably 0.5 to 2.0% (w/w), most preferably 0.5 to 1.0% (w/w) of triethanolamine.
Preferably, the pH of the pharmaceutical composition according to the invention will be between 2 and 9, preferably between 3 and 8 and even more preferably between 3 and 7. In another aspect of the invention, said pharmaceutical composition further comprises isopropyl myristate. Advantageously according to the invention, it was unexpectedly shown that addition of isopropyl myristate leads to markedly improved results when used in conjunction with propylene glycol as a further penetration enhancer. Preferably, said pharmaceutical composition comprises 0.05 to 5.0% (w/w), more preferably 0.1 to 4.0% (w/w), more preferably 0.15 to 3.0% (w/w), even more preferably 0.25 to 2.5% (w/w), even more preferably 0.25 to 2.0% (w/w), even more preferably 0.25 to 1.5% (w/w), still more preferably 0.25 to 1.0% (w/w), most preferably 0.5 to 0.75% (w/w) of isopropyl myristate.
Accordingly, in one embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
In another embodiment, the present invention provides a pharmaceutical composition comprising:
Additionally, said pharmaceutical composition may comprise usual pharmaceutical additives, including salt(s), emollient(s), stabilizer(s), antimicrobial(s), fragrance(s), and/or propellant(s). Said pharmaceutical composition may also include at least one further active ingredient, e.g. another hormone.
The invention also provides a gel useful for transdermal or transcutaneous delivery comprising said pharmaceutical composition according to the invention. Thus, the invention is also directed to a testosterone-containing hydro-alcoholic gel.
According to another embodiment, the present invention provides a dose packet, unit dose packet or multiple dose packet containing said pharmaceutical composition or said hydroalcoholic gel. Advantageously, such conditioning of said pharmaceutical composition renders application easier for a patient. Thus, these forms of packaging can reflect the schedule of application, e.g. daily or weekly administration.
According to another embodiment, there is provide a dispenser, e.g. with hand pump or valve, containing said pharmaceutical composition or said hydroalcoholic gel. Such dispensers allow for flexibility in the administered dosage, as a function of the amount of composition to be applied.
According to one embodiment, said packets or dispensers can be accompanied by a notice giving instructions for the use thereof.
The pharmaceutical compositions, gels, packets and containers of the invention are useful for treating testosterone deficiency, and treating and/or preventing testosterone deficiency-related conditions and/or disorders.
The term ‘treat’ or ‘treatment’ as used herein refers to any treatment of a mammalian condition, disorder, or disease associated with a depressive disorder, and includes, but is not limited to, preventing the condition, disorder, or disease from occurring in a subject which may be predisposed to the condition, disorder, or disease, but has not yet been diagnosed as having the condition, disorder, or disease; inhibiting the condition, disorder, or disease, for example, arresting the development of the condition, disorder, or disease; relieving the condition, disorder, or disease, for example, causing regression of the condition, disorder, or disease; or relieving the condition caused by the disease or disorder, for example, stopping the symptoms of the disease or disorder.
The term ‘prevent’ or ‘prevention’, in relation to a condition, disorder, or disease, means no condition, disorder, or disease development if none had occurred, or no further condition, disorder, or disease development if there had already been development of the condition, disorder, or disease.
As used herein, ‘testosterone deficiency’ refers to lower serum levels of free testosterone in a subject as compared to the median serum levels for healthy subject of the same age. For example, normal cycling women produce approximately 300 μg of testosterone per day. Their total serum testosterone levels generally range from about 20 ng/dL to about 80 ng/dL averaging about 40 ng/dL. In healthy young women, for example, mean free testosterone levels are generally about 3.6 pg/mL. However, several factors may influence both total and free testosterone serum levels. For example, in regularly ovulating women, there is a small but significant increase in plasma testosterone levels during the middle third of the menstrual cycle. However, mean testosterone levels (1.2 nmol/L or 33 ng/dL) and mean free testosterone levels (12.8 pmo/L or 3.6 pg/mL) during the luteal and follicular phases are not significantly different. Additionally, testosterone production declines continuously after age 30 so that serum testosterone levels in a 60-year-old woman are only 50% of the levels in a young 30-year-old woman. Although the percentage of free testosterone generally does not vary with age, an absolute decline in free testosterone has been observed. This decline does not occur abruptly at menopause but instead occurs gradually and continuously as a result of the age-related decrease in both the adrenal and ovarian androgen production. Thus, women begin to experience symptoms associated with menopause in the immediate pre-menopausal years. The decline in testosterone following menopause results from the combination of ovarian failure, decreasing renal secretion, and peripheral conversion. Also, for example, after ovariectomy, testosterone concentrations decrease by about 50%. Diagnosis of a testosterone deficiency is known to the average physician practicing in the relevant field of medicine.
The pharmaceutical compositions and gels of the present invention are also useful to treat a number of physiological and psychological parameters and/or conditions associated with testosterone deficiency in a man or a woman. For example, the pharmaceutical compositions and gels of the present invention are useful for:
The present pharmaceutical compositions and gels can also be used in “combination therapy” with another hormone or steroid, or a pharmaceutical agent that increases testosterone levels in a subject, or an estrogenic hormone, or another pharmaceutical agent such as, for example, an antidepressant agent.
The invention also relates to a process for preparing a pharmaceutical composition, e.g. in the form of a gel or a solution, according to the invention.
In one aspect, said process comprises the step of dissolving testosterone, with stirring, in a solvent of at least one C2-C6 alcohol and at least one penetration enhancer (e.g. propylene glycol, and/or isopropyl myristate).
In another aspect, said process comprises the step of adding water, with stirring, to the mixture obtained.
Where the preparation of a gel is desired, at least one gelling agent, such as Carbopol®, is then added to the mixture, with stirring.
Optionally, a base/neutralizer such as triethanolamine is added to the mixture, with stirring.
In another aspect, the invention relates to a process for preparing a pharmaceutical composition or a gel according to the invention, comprising the steps of:
In another aspect, the invention also relates to the use of the gel or the solution according to the invention for the preparation of a medicinal product for transdermal application for the treatment of a physiological condition associated with an androgen/testosterone deficiency.
In another aspect, the present invention relates to a method of treatment.
In one embodiment, said method comprises the step of administering testosterone, to a subject in need thereof.
According to one aspect, the pharmaceutical composition or the gel of the present invention is applied on healthy skin, e.g. to the shoulder, or to the external part of the arm, thigh or leg.
In order to measure and determine the amount of testosterone to be delivered to a subject in need thereof, serum testosterone concentrations can be measured using standard assay techniques.
Therefrom, the skilled person would know how to determine the amount of composition to be administered, depending upon the exact testosterone dosage of said pharmaceutical composition.
In general, the usual daily dose of the pharmaceutical composition in the form of a gel or a solution according to the invention is between 2.5 g and 5 g of the formulation per day.
The advantages of the invention will become apparent from the following examples, which are given below as mere illustrations, and are non limitative.
The skilled person will appreciate that the present invention can incorporate any number of the preferred features described above.
All citations mentioned herein are hereby incorporated by reference in their entirety. Other embodiments of the present invention are not presented here, which are obvious to those skilled in the art, and thus are within the scope and the spirit of the present invention. The practice of the present invention will employ, unless otherwise indicated, conventional techniques of pharmacology and pharmaceutics, which are within the skill of the art.
Studies on transcutaneous delivery of testosterone gel formulations were carried out on animal and human skin using radio-labelled (14C) testosterone and liquid scintillation titration. These studies give access to kinetics parameters (released amount after 24 h and flux). Two aspects were investigated:
The gelling agent (polymer) is swelled overnight in the required amount of water. Labeled and non-labeled testosterone is dissolved in ethanol, possibly with penetration enhancer. The aqueous and alcoholic phases are mixed, then neutralized with triethanolamine. Mixing is carried out with a spatula until perfect homogeneity is reached. Seventeen formulations were evaluated for transcutaneous delivery. The composition thereof is given in the table below (in % wt based on total weight of formulation). Formulations 13 and 14 correspond to non-gelled solutions.
(Carb. = Carbopol ®)
3. Dermal Permeation Kinetics Protocol
3.1 Dermal Permeation Cell
Static flux cells comprising two unequal compartments are used (
3.2 Biological Membrane
Animal Skin
Skin biopsies of male naked rat skin (IOPS rats, 5 weeks) are sampled at the ventral level. Fat tissues located to the inner face are removed, and the skin is cut in order to obtain samples with an area greater than 2.54 cm2. Each sample is stuck to the lower compartment with the skin external face upwards.
Human Skin
Experiments are carried out on surgical plastics sampled at the abdomen level from a white 35 year old female. Samples are used immediately after sampling and dermatomed on a thickness of about 250 μm.
3.3 Study Protocol
The two compartments 1, 4 of the dermal permeability cell are assembled with the skin fragment 2. The receiving compartment 4 is filled with a precisely measured volume of a 0.5% albumin solution. The cell is then placed for 15 h in a thermostatic bath at 37° C. on a stirring platform in order to obtain equilibrium between the skin and the receiving medium.
Kinetics starts after application of the formulation onto the skin 2. Applications are about 20 mg on 2.54 cm2 of skin. Over a period of 24 h, 2 mL samples are carried out from the receiving compartment 4 at evenly spaced time points, and 2 mL fresh albumin solution is immediately added to the compartment in order to keep its volume constant. For each formulation, five permeation cells are used. After cold-storing, the samples are titrated by liquid scintillation.
4. 14C Testosterone Titration
The samples are split into two 1 mL fractions to which 4 mL scintillation liquid (Pico Fluor 40®) are added, metering time is 5 min.
Samples are then titrated by liquid scintillation using a β spectrophotometre (Beckman). For scintillation, quenching leads to a decreased detection efficiency, which is accurately evaluated establishing a calibration curve termed quenching curve. For each sample, the metering yield depending upon quenching is calculated using an external standard source and the actual radioactivity is determined.
Results
1. Testosterone Solubility
A known amount of solvent (2 mL) is stirred at 37° C. in a closed Erlenmeyer. Starting from a known amount of testosterone, fractions are progressively added. The remaining amount of testosterone is weighted in order to determine the amount in the Erlenmeyer (by difference).
Testosterone solubility at 37° C. in water/ethanol mixtures and various penetration enhancers such as propylene glycol (PG), oleic acid (OA), propylene glycol dipelargonate (PGDP), transcutol, are determined by the increment method.
Testosterone solubility is also evaluated as a function of ethanol content.
See plots on
2. Gel Viscosity
Gel rheology was characterized with a Carri-Med controlled-stress rheometer, measurement geometry: plane/plane, diameter 2 cm, gap 1 mm, temperature 20° C.
Rheograms are plotted in flow following a defined shear cycle as follows:
All gels had a shear softening (pseudo-plastic) behavior, without any hysteresis cycle.
Two rheological studies were carried out:
The influence of ethanol content on Carbopol® hydroalcoholic gels is studied by varying from 50% to 70% the content in 95% ethanol.
Above 55% ethanol, viscosity is smaller, with a strong variation as a function of ethanol content. Viscosity is increased for 50% ethanol.
2.2 Carbopol® 980 Gel Viscosity
The influence of polymer content on hydroalcoholic gels with 40% ethanol is studied by varying Carbopol®980 content from 0.3% to 0.7%.
Viscosities are measured in the plateau under 300 N/m2 stress for 1 min.
3. Dermal Permeation of Testosterone Formulations on Rat Skin
Dermal permeation for the formulations from Table I is evaluated in vitro on rat skin. Formulation 25 cannot be evaluated due to heterogeneity (phase separation). Results for released amount at 24 h, and released rate at 24 h are given in the table below.
Formulations 11-27: 8 mg/cm2 are deposited.
4. Testosterone Permeation on Human Skin
Formulations 26 and 27 are evaluated on human skin, together with formulation 15 as a reference. Results are detailed below:
Formulation 26
Mean Values for Permeation Parameters on 5 Cells:
Average kinetics plot is given on
Mean flux: 0.26 μg/cm2 · h
Formulation 27
Mean Values for Permeation Parameters on 5 Cells:
Average kinetics plot is given on
Mean flux: 0.39 μg/cm2 · h
Formulation 15 (Reference)
Mean Values for Permeation Parameters on 5 Cells:
Average kinetics plot is given on
Mean flux: 0.15 μg/cm2 · h
1. Material
[1,2,6,7−3H] testosterone (Amersham Laboratories): MW=295, solution at 1 mCi/mL (37 MBq/mL) in ethanol; specific activity 99 Ci/mmol (3.66 TBq/mmol), i.e. 336 mCi/mg (lot 18).
Absolute ethanol Carlo Erba lot V4N051154N
Formulations to be applied onto skin: (in weight for 100 g final formulation).
Formulations are Prepared as Follows:
Formulation 1:
1 g of radio-labelled gel is prepared as follows.
1g of radio-labelled gel is prepared according to the following protocol:
1 g of radio-labelled gel is prepared according to the following protocol:
1 g of radio-labelled gel is prepared according to the following protocol:
1 g of radio-labelled gel is prepared according to the following protocol:
In vitro transdermal absorption is quantitatively studied on human ventral dermatomed biopsies placed in a static diffusion cell (Franz cell), which allows contacting dermis with a survival liquid (receptor fluid) in which absorption through skin is to be dosed.
Cell
A dermal biopsy is maintained horizontally between two parts of the cell, thus delimiting two compartments:
The two elements are assembled via a clamp.
The lower compartment (dermal) is filled with a survival liquid constituted of a sodium chloride solution at 9 g/L supplemented with bovine serum albumin at 15 g/L. At each time point, the survival liquid is entirely sampled out by the lateral collection port and is replaced by fresh liquid.
The lower part of the cell is thermostated at 37° C. Homogeneity of the temperature and the content in the receptor fluid, is maintained by stirring (magnetic stirrer).
The upper part (epidermal compartment) is open towards the exterior, thus exposing the epidermal surface to the air in the laboratory.
Preparation of Human Ventral Dermatomed Skin Dermal Biopsies:
These are samples from human ventral skin from plastic surgery from white donors. Skin is kept at −20° C. before use. Adherent sub-dermal fat is removed with a scalpel, and skin is brought to a thickness of about 0.5 mm with a dermatome. For each assay, the various skin samples are equally distributed between the lots.
General Protocol
Franz cells are usually installed the day before loading the formulation to be studied. The epidermal compartment is contacted with the atmosphere in the laboratory, the dermal compartment is thermostated to 37° C. and the skin is contacted with albuminated physiological serum for about 17 hours. Under these conditions, the skin is highly hydrated.
10 μL solution (about 8 μL, about 8 μCi) are applied with a micropipette onto the whole of the surface of the epidermis delimited by the glass cylinder. Sampling from the liquid contained in the dermal compartment are carried out via the lateral collection port at time points 2 h, 4 h, 6 h, 8 h, and 24 h. For each time point, the survival liquid is entirely sampled out and replace by fresh liquid.
At the end of the assay, the treated dermal surface is washed with 200 μL of various solvents according to the following protocol:
The application area is then wiped with a Q-tip®.
The epidermis and the dermis are then mechanically separated with a scalpel, and digested in respectively in 1 mL and 3 mL Soluene® (Packard).
Radioactivity Measurements
Detection is carried out by liquid scintillation using a particle counter Packard-tricarb 4530.
Preparation of Radioactive Samples:
The survival liquid sampled from the lower compartment of the diffusion cells is directly incorporated in 15 mL of liquid scintillation cocktail (Picofluor 40R, Packard) and metered for radioactivity measurement.
Liquids from washes and Q-tips® are introduced in a vial containing about 30 mL 95° Ethanol and weighted exactly. After incubating overnight at 4° C., the radioactivity of an exactly weighted aliquot from this dilution is determined following the same procedure as for the survival liquids.
Epidermis and dermis must first be dissolved in strong organic bases. They are dissolved by contacting 24 h at 37° C. with Soluene 350® Packard (1 mL per 100 mg tissue) to which 15 mL liquid scintillation cocktail (Hionic Fluor 30®, Packard) are added, and then metered.
Radioactivity Measurements:
Metering rate is corrected, as far as quenching is concerned, by the method of the external calibration, in order to obtain disintegrations per minute (dpm) accounting for the real activity of each sample. The background is deducted for each sample in cpm. For each scintillation liquid, a specific quenching curve is established.
Results are expressed in weight or percentage of substance found in the samples with respect to the administered amount, determined from the metering rates of suitably diluted calibrations.
Transdermal Absorption:
Intensity of transdermal absorption is evaluated by computing the absorption percentage (%) of the loaded amount (Qi) as a function of time:
%=(Qt/Qi)×100
wherein Qt is the absorbed amount at time point t.
Average flux and absorbed amount for each time interval are also determined and expressed respectively in ng/cm2/h and in ng.
Average results correspond to 6-8 experimental determinations and are associated to the standard deviation (Sd).
Comparison of the average results is carried out by variance analysis.
3. Results
Concentrations expressed as percentages are by weight of substance for 100 g of final formulation.
@: different from reference (variance analysis, Fisher test significant with p < 5%)
*: different from reference (anova; Fisher and Scheffe F test, significant with p < 5%)
29.27%°
E: epidermis, D: dermis, C: cell, W: washes, T: total
@: different from reference (variance analysis, Fisher test significant with p < 5%)
°: different from formulation 2, 3 and 4 (anova, Fisher test, p < 5%)
*: different from reference (anova; Fisher and Scheffe F test, significant with p < 5%)
@: different from reference (variance analysis, Fisher test significant with p < 5%)
*: different from reference (variance analysis, Fisher test and Scheffe F-test significant with p < 5%)
| Number | Date | Country | Kind |
|---|---|---|---|
| 04292170.0 | Sep 2004 | EP | regional |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP05/10293 | 9/7/2005 | WO | 9/24/2007 |
| Number | Date | Country | |
|---|---|---|---|
| 60638360 | Dec 2004 | US |
