Patent Application
20060183719
1. Field of the Invention
The present invention is directed to a metal complex of the compound of Formula (I). More particularly, the present invention is directed to a solid dosage form of a metal complex of a tetracycline of Formula (I) and the method of making such solid dosage form.
2. Related Background Art
Tetracyclines are well known antibiotic compounds that have been effectively used in the treatment of vibrio infections, gram-positive, gram-negative, aerobic and anaerobic bacteria, as well as spirochetes, mycoplasmas, rickettsiae, chlamydiae and some protozoans. Tetracyclines work by inhibiting enzyme reactions of bacterial cells, such as protein synthesis. It is commonly used in the treatment of bacterial infections caused by these organisms, such as urinary tract infections, upper respiratory tract infections, acne, gonorrhea, chlamydia, anthrax, lyme disease and others.
When administered orally, tetracyclines are absorbed in varying degrees. For example, 60-80% absorption is observed for demeclocycline, oxycycline, and tetracycline. While doxycycline and minocycline are absorbed at a higher level of about 90%.
It is widely held that tetracyclines should not be taken simultaneously with iron supplements, multivitamins, calcium supplements, antacids, or laxatives. Many believe that the efficacy and absorption of the tetracycline in the body decreases when taken simultaneously or in conjunction with one of the above noted products.
British Patent No. 1,360,998 to Villax describes a process for isolation of α-6-deoxytetracyclines from a crude reaction mixture. Villax also discloses that oral pharmaceutical suspensions may be prepared using the calcium salts of tetracyclines. The process taught by Villax uses methanol, i.e., an organic solvent. While the use of an organic solvent is not uncommon in preparing pharmaceutically active ingredients, it is generally preferred not to use them for preparing a solid dosage form. Therefore, the process disclosed in Villax for isolating deoxytetracycline salts would likely contain residual solvent, thus making this process unacceptable for producing a solid dosage form suitable for pharmaceutical administration.
Currently, only a suspension of a calcium salt of a tetracycline is available. Pharmaceutical suspensions, however, often suffer from a lack of patient compliance. In addition, many individuals do not prefer a liquid dosage form and find it inconvenient to use.
However, making a solid dosage form of a tetracycline metal complex is extremely difficult. Processing hurdles exist and have not been adequately resolved. For example, a method of collecting the tetracycline metal complex from the aqueous suspension has not been achieved. Accordingly, it would be highly desirable to effectively produce a solid dosage form of a tetracycline metal complex.
The present invention is directed to a solid dosage form of a metal complex of a tetracycline of Formula (I) for pharmaceutical administration,
wherein
R1=Cl, N(CH3)2, or H;
R2=CH3, H, or CH2═;
R3=CH3, H, OH, or absent; and
R4=OH or H,
with the proviso that if R2 is CH3 and R3 is H, then R4 is not OH.
A process for making a metal complex of the compound of Formula (I) in a solid dosage form is also included in the present invention. The process comprises the steps of (i) providing an aqueous solution of the compound of Formula (I) or a physiologically acceptable salt thereof, where the compound is partially or completely solubilized; (ii) admixing a metal salt with the aqueous solution; (iii) admixing a suitable base to increase the pH of the aqueous solution, whereby a suspension of the metal complex of the compound of Formula (I) is formed after steps (ii) and (iii); and (iv) drying the suspension of the metal complex of the compound of Formula (I). The process may include the step of admixing one or more pharmaceutically acceptable excipients to form a granulation. The excipient may be added prior to the step of drying the suspension. A further step may involve filling the granulation into capsules or compressing the granulation into tablets.
In another embodiment, the process for making a metal complex of the compound of Formula (I) in a solid dosage form comprises the steps of (i) providing an aqueous solution of Formula (I) or a physiologically acceptable salt thereof, where the compound is partially or completely solubilized; (ii) admixing a metal salt with the aqueous solution; (iii) admixing a suitable base to increase the pH of the aqueous solution, whereby a suspension of the metal complex of the compound of Formula (I) is formed after steps (ii) and (iii); (iv) admixing one or more pharmaceutically acceptable-excipients with the suspension, thereby forming a wet granulation; and (v) drying the wet granulation, thereby forming a dry granulation of the metal complex of the compound of Formula (I). The process may include, the step of admixing one or more pharmaceutically acceptable-excipients after the wet granulation is formed. In addition, the dry granulation may be filled into capsules or compressed into tablets.
The tetracycline metal complex of Formula (I) may be used in treating bacterial infections. The method of treatment comprises the step of administering an effective amount of a solid dosage form comprising a metal complex of a compound of Formula (I) for an effective period of time, to a host in need thereof.
In addition, the invention includes a method for treating ailments resulting from microorganisms and/or bacteria, comprising the step of administering a safe and effective amount of a metal complex of a tetracycline of Formula (I) in a solid dosage form for an effective period of time, to a host in need thereof.
For the purposes of the present invention, all percentages provided are by weight, unless otherwise specified.
The tetracycline metal complex of Formula (I) and the active ingredients of the present invention are used in a “safe and effective amount.” This is understood to mean a sufficient amount of a compound or composition that will positively modify the symptoms and/or condition to be treated, with the understanding that the amount is low enough to avoid serious side effects. The amount of the compound that is considered safe and effective will depend upon several factors. For example, the condition and severity of the condition being treated, the age, body weight, general health, sex, diet, and physical condition of the patient being treated, the duration of the treatment, the nature of concurrent therapy, the particular active ingredient being employed, the particular pharmaceutically-acceptable excipients utilized, the time of administration, method of administration, rate of excretion, drug combination, and any other relevant factors should be given consideration.
The term “pharmaceutically-acceptable excipient” is understood to mean any physiologically inert, pharmacologically inactive material known to one skilled in the art, which is compatible with the physical and chemical characteristics of the particular tetracycline metal complex of Formula (I) selected for use.
Tetracyclines represented by Formula (I) can be combined with metal salts, such as calcium chloride, to produce metal complexes. Formula (I) is represented by the structure
wherein
R1=Cl, N(CH3)2, or H;
R2=CH3, H, or CH2═;
R3=CH3, H, OH, or absent; and
R4=OH or H,
with the proviso that if R2 is CH3 and R3 is H, then R4 is not OH.
An important aspect of the present invention is that the metal complex is formed in accordance to a molar ratio of tetracycline to metal ion ranging from about 3:1 to about 1:3. More preferably, the molar ratio is from about 2:1 to 1:1.
The present invention is directed to a solid dosage form of a metal complex of a tetracycline of Formula (I) for pharmaceutically acceptable administration to a user/patient, i.e., any residual solvents or other impurities are at a level that is considered safe for human consumption. In one embodiment, Formula (I) is the compound tetracycline where R1=H, R2=CH3, R3=OH, and R4=H.
In another embodiment, Formula (I) is chlortetracycline where R1=Cl, R2=CH3, R3=OH, and R4=H.
In yet another embodiment, Formula (I) is oxytetracycline where R1=H, R2=CH3, R3=OH, and R4=OH.
In still yet another embodiment, Formula (I) is demeclocycline where R1=Cl, R2=H, R3=OH, and R4=H.
In still yet another embodiment, Formula (I) is methacycline where R1=H, R2=CH2═, R3 is absent, and R4=OH.
In still yet another embodiment, Formula (I) is lymecycline (N-Lysinomethyl tetracycline) where R1=H, R2=OH, R3=CH3, and R4=H.
In a preferred embodiment, Formula (I) is minocycline where R1=N(CH3)2, and R2, R3 and R4=H.
The metal complex of the compound of Formula (I) may be, for example, a calcium complex, a magnesium complex, a sodium complex, a zinc complex, an aluminum complex, an iron complex, a copper complex or mixtures thereof. Preferably, the metal complex is a calcium complex.
It is desirable that the tetracycline metal complex of the compound of Formula (I) undergo substantially complete complexation, where at least about 75% by weight of the tetracycline of Formula (I) has complexed. More preferably at least about 90% has complexed. It should be understood, however, that an excess of the tetracycline of Formula (I) or metal salt may be added to form the metal complex of the compound of Formula (I).
Conventional wisdom dictates that the presence of a metal source with a tetracycline compound can interfere or inhibit the absorption of the tetracycline. Literature sources advise against the administration of the tetracycline with a calcium, iron, magnesium, or zinc supplement or product. Surprisingly, however, the inventors theorize that the body can effectively absorb a tetracycline from a metal complex of a tetracycline compound of Formula (I) in a solid dosage form.
Another aspect of the present invention is that the solid dosage form of the metal complex of the compound of Formula (I) can be engineered to exhibit a desired release profile. For example, the solid dosage form may be engineered to exhibit an immediate release, a controlled release, or a sustained release of the tetracycline compound of Formula (I). This may be accomplished, for example, by measuring the dissociation rates of the various metal complexes of the tetracycline compound of Formula (I) and then carefully selecting a particular complex based upon its properties. Moreover, two or more complexes may be combined to create the desired release profile.
The solid dosage form of the present invention may be a powder, capsule, tablet, coated tablet, aerosol, pellet, chewable tablet, lozenge, gelatin filled capsule, and the like.
The pharmaceutical dosage form of the present invention may be formulated together with one or more pharmaceutically acceptable carriers or excipients and/or bioactive agents.
Suitable pharmaceutically-acceptable excipients include, but are not limited to, polymers, resins, plasticizers, fillers, lubricants, binders, disintegrants, granulating agents, solvents, co-solvents, surfactants, preservatives, sweetening agents, flavoring agents, buffering systems, antioxidants, pharmaceutical grade dyes, pigments, and mixtures thereof.
Polymers that may be used, include but are not limited to, hydroxypropylmethylcellulose (HPMC) alone and/or in combination with hydroxypropylcellulose (HPC), carboxymethylcellulose, acrylic resins such as Eudragit®, methylcellulose, ethylcellulose, and polyvinylpyrrolidone or other commercially available film-coating preparations.
Suitable plasticizers, include but are not limited to, polyethylene glycol, propylene glycol, dibutyl phthalate, castor oil, acetylated monoglycerides, triacetin, and mixtures thereof.
Examples of fillers, include but are not limited to, lactose, sucrose, maltodextrin, mannitol, starch, microcrystalline cellulose, and mixtures thereof.
Lubricants that may be used, include but are not limited to, magnesium stearate, stearic acid, talc, and mixtures thereof.
Suitable binders, include but are not limited to, methycellulose, sodium carboxymethycellulose, hydroxypropylmethylcellulose, carbomer, povidone, acacia, guar gum, xanthan gum, tragacanth, calcium silicate, magnesium aluminum silicate, ethylcellulose, pregelatinized starch, and mixtures thereof. Particularly preferred are methycellulose, carbomer, xanthan gum, guar gum, povidone and sodium carboxymethycellulose.
Disintegrants that may be used, include but are not limited to, crospovidone, sodium carboxymethyl starch, sodium starch glycolate, sodium carboxymethyl cellulose, alginic acid, clays, ion exchange resins, and mixtures thereof.
Examples of surfactants, include but are not limited to, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene monoalkyl ethers, sucrose monoesters, lanolin esters and ethers, and mixtures thereof.
Suitable preservatives, include but are not limited to, phenol, alkyl esters of parahydroxybenzoic acid, benzoic acid and the salts thereof, boric acid and the salts thereof, sorbic acid and the salts thereof, chlorbutanol, benzyl alcohol, thimerosal, phenylmercuric acetate and nitrate, nitromersol, benzalkonium chloride, cetylpyridinium chloride, methyl paraben, propyl paraben, and mixtures thereof. Particularly preferred are the salts of benzoic acid, cetylpyridinium chloride, methyl paraben and propyl paraben.
Antioxidants that may be used, include but are not limited to, tocopherols and derivatives thereof, ascorbic acid, beta-carotene, selenium, sodium bisulfite, sodium metabisulfite, ascorbyl palmitate, citric acid, catechins and derivatives thereof, and mixtures thereof.
Suitable sweeteners, include but are not limited to, sucrose, glucose, saccharin, aspartame, sorbitol, xylitol, sucralose and mixtures thereof. Particularly preferred are sucrose and sucralose.
Buffering systems that may be used include, but are not limited to, potassium acetate, boric carbonic, phosphoric, succinic, malic, tartaric, citric, acetic, benzoic, lactic, glyceric, gluconic, glutaric, glutamic, and mixtures thereof. Particularly preferred are phosphoric, tartaric, citric, and potassium acetate.
A variety of solvents may be used. However, water is the preferred solvent. The water may be acidified with an acidifying agent. Inorganic acidifying agents include, but are not limited to, hydrochloric acid, sulfuric acid, nitric acid, and mixtures thereof. Alternatively, organic acidifying agents may be used, such as for example, acetic acid, ethanoic acid, and mixtures thereof. Preferably the acidifying agent is hydrochloric acid.
Suitable co-solvents, include but are not limited to, ethanol, glycerin, propylene glycol, polyethylene glycol, and mixtures thereof.
The pharmaceutical compositions described herein are comprised of from about 0.1 weight percent (wt. %) to about 99.9 wt. %, preferably from about 5.0 wt. % to about 50.0 wt. %, and most preferably from about 10 wt. % to about 50 wt. % of the tetracycline metal complex of Formula (I), and from about 0.1 wt. % to about 99.9 wt. %, preferably from about 5.0 wt. % to about 99.9 wt. %, and most preferably from about 50 wt. % to about 90 wt. % of one or more pharmaceutically-acceptable excipients.
The solid dosage form of the metal complex of the compound of Formula (I) maybe made using a process comprising the steps of (i) providing an aqueous solution of a tetracycline of Formula (I) or a physiologically acceptable salt thereof, where the compound is partially or completely solubilized; (ii) admixing a metal salt to the aqueous solution, (iii) admixing a base to increase the pH of the aqueous solution, whereby a suspension of a tetracycline metal complex of Formula (I) is formed after steps (ii) and (iii); and (iv) drying the suspension of the tetracycline metal complex of Formula (I). Additionally, one or more pharmaceutically acceptable-excipients may be admixed, before, during, or after each processing step.
Examples of the physiologically acceptable salts, include, but are not limited to, hyclates, monohydrates, carrageenates, hydrochlorides or phosphates of the tetracycline of Formula (I). The tetracycline of Formula (I) or physiologically acceptable salts thereof are partially or completely dissolved in an aqueous solution. Any suitable means may be used to dissolve the tetracycline of Formula (I) or its salt in the aqueous solution. Generally, all that is required is some form of mixing. Other components, such as ethanol or acid, may be included in the aqueous solution. The aqueous solution generally will have a pH that is in a range of about 0.5 to about 8.
Suitable metal salts include but are not limited to, calcium salts, sodium salts, magnesium salts, zinc salts, and mixtures thereof. A particularly preferred metal salt is calcium chloride. The metal salt may be added with or without mixing, but is generally mixed into the aqueous solution.
About 1 wt. % to about 70 wt. %, preferably about 10 wt. % to about 50 wt. %, and more preferably about 35 wt. % to about 50 wt. % of the metal salt solution is added to the aqueous solution, whereby the ratio of metal to tetracycline is from about 10:1 to about 0.5:1, such that the resulting complex has a molar ratio of tetracycline to metal ion ranging from about 3:1 to about 1:3.
A pharmaceutically acceptable base is added to the aqueous solution and mixed with the other components. Examples of pharmaceutically acceptable bases capable of forming salts for the purpose of the present invention, include, but are not limited to, alkali metal bases, such as sodium hydroxide, potassium hydroxide, lithium hydroxide and the like; alkaline earth metal bases, such as calcium hydroxide, barium hydroxide and the like; and ammonium hydroxide. Alkali metal or alkaline earth metal salts suitable for forming pharmaceutically acceptable salts can include anions such as carbonates, bicarbonates such as sodium bicarbonate and sulfates. Additional suitable bases include for example, triethanolamine, diethanolamine, monoethanolamine, triethylamine and mixtures thereof.
The base is added in an amount effective to form a suspension of the metal complex of the compound of Formula (I). Generally the addition of the base raises the pH of the solution to a pH range of about 2 to about 14, preferably about 5 to about 10, and most preferably about 5 to about 7. Several factors are used to determine the amount of base that will be added, including the pH of the aqueous solution, the pKa(s) of the tetracycline, and the equilibrium constant of the tetracycline metal complex.
The suspension of the metal complex of the compound of Formula (I) is formed after the addition of the metal salt and the suitable base. It should be understood, however, that the order of addition of these ingredients may be varied. In a preferred embodiment, the metal salt is added and mixed into the aqueous solution prior to the addition of the suitable base.
Drying the suspension may be carried out using a variety of techniques. For example in one embodiment, a spray dryer is used and the suspension is sprayed onto an excipient. Other methods include decanting, evaporation, freeze drying, tray drying, fluid-bed drying, and the like.
In an alternative embodiment, the process for making a solid dosage form of a metal complex of the compound of Formula (I) comprises the steps of: (i) providing an aqueous solution of the compound of Formula (I) or a physiologically acceptable salt thereof, where the compound is partially or completely solubilized; (ii) admixing a metal salt with the aqueous solution, (iii) admixing a base to increase the pH of the solution, whereby a suspension of the metal complex with the compound of Formula (I) is formed after steps (ii) and (iii); (iv) admixing one or more pharmaceutically acceptable-excipients with the suspension, thereby forming a wet granulation; and (v) drying the wet granulation, thereby forming a dry granulation of the metal complex of the compound of Formula (I). Steps (i)-(iii) of this embodiment are the same as the prior described process that forms the solid dosage form from the suspension.
This embodiment, however, utilizes an excipient to assist in forming a wet granulation. The inventors have discovered that an excipient, e.g., microcrystalline cellulose, can be used to absorb and adsorb the moisture in the suspension when admixed with the suspension. The result is a wet granulation or slurry that has about 5 wt. % to about 99 wt. % water. Preferably, the moisture in the wet granulation is about 25 wt. % to about 60 wt. %.
One or more pharmaceutically acceptable-excipients can be admixed in either process during any of the process steps. Moreover, excipients can be added in more than one step.
The wet granulation may be dried by tray-drying, fluid-bed drying, decanting, evaporating, freeze drying, a combination thereof, or by other processes known to those skilled in the art.
To ensure that the solid dosage form is suitably stable, it is desirable that the granulation of the metal complex of the compound of Formula (I) be dried to a moisture content of about 1 wt. % to about 15 wt. % based on the total weight of the metal complex granulation. More preferably, the moisture content should be less than about 10 wt. %. Most preferably, to about 1 wt. % to about 6 wt. %.
The resulting metal complex of the compound of Formula (I) is in the form of a dry granulation consisting of granules and powder. The dry granulation may be blended with excipients such as lubricants, e.g., magnesium stearate. The final blend may be further processed, for example, by filling it into capsules.
The solid dosage form is generally administrated orally. Suitable forms include, but are not limited to, tablets, capsules, powders, granules, lozenges, aerosols, pellets, chewable tablets, and the like.
The inventors have also discovered that the granulation formed after the drying step is well suited for tableting. In fact, tablets can be made directly from the metal complex suspension, making the process more efficient. However, it should be understood that tableting excipients may be incorporated. As previously noted, the excipients, such as lubricants, may be added before and/or after the step of drying the suspension or wet granulation. The excipients are simply added and mixed with the other components.
In a preferred embodiment, the tablets are chewable tablets. Moreover, the inventors have discovered that the insoluble nature of the metal complex helps mask the unpleasant taste of the tetracycline compound of Formula (I). Typically, these compounds are known to have an extremely bitter taste.
In addition, the solid dosage form of the present invention may contain additional ingredients. For example, the additional ingredients may include natural and artificial flavors, sweeteners, colorings, coating excipients, binders, disintegrants, lubricants, and the like.
Excipents such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinyl-pyrrolidone; fillers for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tableting lubricant, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants for example potato starch, or acceptable wetting agents such as sodium lauryl sulphate, may be included to facilitate the formation of the tablet. In addition, coatings may be applied over the tablets using methods known in the art.
Bacterial infections may be treated using the present invention. The method of treatment comprises the step of administering to a host, such as a human or animal, a safe and effective amount of a tetracycline metal complex, in solid dosage form. Examples of bacterial infections that can be treated with a tetracycline metal complex, include urinary tract infections, upper respiratory tract infections, acne, gonorrhea, chlamydia, syphilis, anthrax, lyme disease and the like.
The present invention may also be used in the treatment of ailments caused by bacteria and microorganisms. Non-limiting examples of bacteria and microorganisms are gram-positive microorganisms, gram-negative microorganisms, aerobic bacteria, anaerobic bacteria, spirochetes, mycoplasmas, rickettsiae, chlamydiae, treponema, listeria, bacillus anthracis, fusobacterium fusiforme, actinomyces israelii, clostridium, ureaplasma urealyticum, borrelia recurrentis, haemophilus ducreyi, yersinia pestis, francisella tularensis, vibrio cholerae, brucella, campylobacter fetus, bartonella bacilliformis, calymmatobacterium granulomatis, and protozoans. The method of treatment comprises require administering a safe and effective amount of a solid dosage form of a tetracycline metal complex for an effective period of time.
83 grams of minocycline hydrochloride was added to 248 grams of water. Hydrochloric acid was then added to adjust the pH to less than 1. Next, 37.3 grams of 40% w/w calcium chloride solution was added. The solution was then mixed. This step was followed by adding 130 grams of 5N sodium hydroxide solution. Addition of the base, formed and precipitated a minocycline calcium complex suspension, which had a final pH in the range of from about 5 to less than about 8.
600 grams of microcrystalline cellulose is added to a suspension of minocycline calcium complex made following the procedure of Example 1. The suspension is mixed to form a wet granulation. The wet granulation is subjected to a drying operation using a tray dryer until the moisture content is less than about 5% w/w. The dried granulation is then passed through a mill, to reduce the particle size of the granulation to a particle size suitable for making into a solid dosage form. Next, 2 grams of magnesium stearate is added and blended into the mixture. Finally, the mixture is compressed into tablets containing 75 mg of minocycline. The tablets may then be coated with a film coating.
It should be understood that different levels of minocycline may be delivered in tablet form.
The procedure of Example 1 is followed to form a minocycline calcium complex. 500 grams of microcrystalline cellulose is then mixed into the minocycline calcium complex that is formed, resulting in a wet granulation. The wet granulation is dried in a tray dryer until the moisture content is less than about 5 wt. %. The dried granulation is then passed through a mill, to reduce the particle of the granulation to a particles size suitable for making into a solid dosage form. Next, 2 grams of magnesium stearate is added and blended into the mixture. The dry granulation is then filled into size 00 hard gelatin capsules, where each capsule contains 50 mg of minocycline.
It should be understood that different levels of minocycline may be delivered in capsule form.
83 grams of minocycline hydrochloride was added to 248 grams of purified water. Hydrochloric acid (37% w/w) was then added to lower the pH to less than 1. Next 20 g of calcium chloride dihydrate was added and mixed. This step was followed by adding and mixing 201 grams of 20% w/w sodium hydroxide solution. Addition of the base formed and precipitated a minocycline calcium complex suspension. The suspension that was formed had a pH reading of 12. Next, 42 grams of HCl (37% w/w) was added and mixed. The final pH of the suspension was 7.
25 g of croscarmellose sodium and 175 g microcrystalline cellulose are added to a suspension of minocycline calcium complex made following the procedure of EXAMPLE 1 or EXAMPLE 4. The suspension is mixed to form a wet granulation. The wet granulation is subjected to a drying operation using a fluid bed dryer until the moisture content is less than about 5% w/w. The dried granulation is then passed through a mill, to reduce the particle of the granulation to a particles size suitable for making into a solid dosage form. Next 150 grams of microcrystalline cellulose is added and blended into the mixture. This is followed by adding and blending 2 grams magnesium stearate into the mixture. Finally, the mixture is compressed into tablets containing 100 mg of minocycline. The tablets may then be coated with a film coating.
It should be understood that different levels of minocycline may be delivered in tablet form.
15 grams of croscarmellose sodium, 30 grams of starch, 4.5 grams of citric acid and 150 grams of microcrystalline cellulose are added to a suspension of minocycline calcium complex made following the procedure of EXAMPLE 1 or EXAMPLE 4. The suspension is mixed to form a wet granulation. The wet granulation is subjected to a drying operation using a fluid bed dryer until the moisture content is less than about 5% w/w. The dried granulation is then passed through a mill, to reduce the particle of the granulation to a particles size suitable for making into a solid dosage form. Next 150 grams of microcrystalline cellulose is added and blended into the mixture. This is followed by adding and blending 2 grams of magnesium stearate into the mixture. Finally, the mixture is compressed into tablets containing 100 mg of minocycline. The tablets may then be coated with a film coating.
It should be understood that different levels of minocycline may be delivered in tablet form.
While the invention has been described above with reference to specific embodiments thereof, it is apparent that many changes, modifications, and variations can be made without departing from the inventive concept disclosed herein. Accordingly, it is intended to embrace all such changes, modifications, and variations that fall within the spirit and broad scope of the appended claims. All patent applications, patents, and other publications cited herein are incorporated by reference in their entirety.
This application claims the benefit of U.S. Provisional Application Ser. No. 60/646,357 filed on Jan. 21, 2005.
| Number | Date | Country | |
|---|---|---|---|
| 60646357 | Jan 2005 | US |
