Patent Grant
11104674
The present invention relates to organic compounds useful for therapy and/or prophylaxis in a mammal, and in particular to HBsAg (HBV Surface antigen) inhibitors and HBV DNA production inhibitors useful for treating HBV infection.
The present invention relates to novel tetrahydropyridopyrimidines having pharmaceutical activity, their manufacture, pharmaceutical compositions containing them and their potential use as medicaments.
The present invention relates to compounds of formula I,
wherein R1 to R4, A, W, Q and Y are as described below, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof.
The hepatitis B virus (HBV) is an enveloped, partially double-stranded DNA virus. The compact 3.2 kb HBV genome consists of four overlapping open reading frames (ORF), which encode for the core, polymerase (Pol), envelope and X-proteins. The Pol ORF is the longest and the envelope ORF is located within it, while the X and core ORFs overlap with the Pol ORF. The lifecycle of HBV has two main events: 1) generation of closed circular DNA (cccDNA) from relaxed circular (RC DNA), and 2) reverse transcription of pregenomic RNA (pgRNA) to produce RC DNA. Prior to the infection of host cells, the HBV genome exists within the virion as RC DNA. It has been determined that HBV virions are able to gain entry into host cells by non-specifically binding to the negatively charged proteoglycans present on the surface of human hepatocytes (Schulze, A., P. Gripon & S. Urban. Hepatology, 46, (2007), 1759-68) and via the specific binding of HBV surface antigens (HBsAg) to the hepatocyte sodium-taurocholate cotransporting polypeptide (NTCP) receptor (Yan, H. et al. J Virol, 87, (2013), 7977-91). Once the virion has entered the cell, the viral cores and the encapsidated RC DNA are transported by host factors, via a nuclear localization signal, into the nucleus through the Impβ/Impα nuclear transport receptors. Inside the nucleus, host DNA repair enzymes convert the RC DNA into cccDNA. cccDNA acts as the template for all viral mRNAs and as such, is responsible for HBV persistence in infected individuals. The transcripts produced from cccDNA are grouped into two categories; Pregenomic RNA (pgRNA) and subgenomic RNA. Subgenomic transcripts encode for the three envelopes (L, M and S) and X proteins, and pgRNA encodes for Pre-Core, Core, and Pol proteins (Quasdorff, M. & U. Protzer. J Viral Hepat, 17, (2010), 527-36). Inhibition of HBV gene expression or HBV RNA synthesis leads to the inhibition of HBV viral replication and antigens production (Mao, R. et al. PLoS Pathog, 9, (2013), e1003494; Mao, R. et al. J Virol, 85, (2011), 1048-57). For instance, IFN-α was shown to inhibit HBV replication and viral HBsAg production by decreasing the transcription of pgRNA and subgenomic RNA from the HBV covalently closed circular DNA (cccDNA) minichromosome. (Belloni, L. et al. J Clin Invest, 122, (2012), 529-37; Mao, R. et al. J Virol, 85, (2011), 1048-57). All HBV viral mRNAs are capped and polyadenylated, and then exported to the cytoplasm for translation. In the cytoplasm, the assembly of new virons is initiated and nascent pgRNA is packaged with viral Pol so that reverse transcription of pgRNA, via a single stranded DNA intermediate, into RC DNA can commence. The mature nucleocapsids containing RC DNA are enveloped with cellular lipids and viral L, M, and S proteins and then the infectious HBV particles are then released by budding at the intracellular membrane (Locarnini, S. Semin Liver Dis, (2005), 25 Suppl 1, 9-19). Interestingly, non-infectious particles are also produced that greatly outnumber the infectious virions. These empty, enveloped particles (L, M and S) are referred to as subviral particles. Importantly, since subviral particles share the same envelope proteins and as infectious particles, it has been surmised that they act as decoys to the host immune system and have been used for HBV vaccines. The S, M, and L envelope proteins are expressed from a single ORF that contains three different start codons. All three proteins share a 226aa sequence, the S-domain, at their C-termini. M and L have additional pre-S domains, Pre-S2 and Pre-S2 and Pre-S1, respectively. However, it is the S-domain that has the HBsAg epitope (Lambert, C. & R. Prange. Virol J, (2007), 4, 45).
The control of viral infection needs a tight surveillance of the host innate immune system which could respond within minutes to hours after infection to impact on the initial growth of the virus and limit the development of a chronic and persistent infection. Despite the available current treatments based on IFN and nucleos(t)ide analogues, the Hepatitis B virus (HBV) infection remains a major health problem worldwide which concerns an estimated 350 million chronic carriers who have a higher risk of liver cirrhosis and hepatocellular carcinoma.
The secretion of antiviral cytokines in response to HBV infection by the hepatocytes and/or the intra-hepatic immune cells plays a central role in the viral clearance of infected liver. However, chronically infected patients only display a weak immune response due to various escape strategies adopted by the virus to counteract the host cell recognition systems and the subsequent antiviral responses.
Many observations showed that several HBV viral proteins could counteract the initial host cellular response by interfering with the viral recognition signaling system and subsequently the interferon (IFN) antiviral activity. Among these, the excessive secretion of HBV empty subviral particles (SVPs, HBsAg) may participate to the maintenance of the immunological tolerant state observed in chronically infected patients (CHB). The persistent exposure to HBsAg and other viral antigens can lead to HBV-specific T-cell deletion or to progressive functional impairment (Nayersina et al. Journal of Immunology (1993), 150, 4659-4671; Kondo et al. Journal of Medical Virology (2004), 74, 425-433; Fisicaro et al. Gastroenterology, (2010), 138, 682-93;). Moreover HBsAg has been reported to suppress the function of immune cells such as monocytes, dendritic cells (DCs) and natural killer (NK) cells by direct interaction (Op den Brouw et al. Immunology, (2009b), 126, 280-9; Woltman et al. PLoS One, (2011), 6, e15324; Shi et al. J Viral Hepat. (2012), 19, e26-33; Kondo et al. ISRN Gasteroenterology, (2013), Article ID 935295).
HBsAg quantification is a significant biomarker for prognosis and treatment response in chronic hepatitis B. However the achievement of HBsAg loss and seroconversion is rarely observed in chronically infected patients but remains the ultimate goal of therapy. Current therapy such as Nucleos(t)ide analogues are molecules that inhibit HBV DNA synthesis but are not directed at reducing HBsAg level. Nucleos(t)ide analogs, even with prolonged therapy, have demonstrated rates of HBsAg clearance comparable to those observed naturally (between −1%-2%) (Janssen et al. Lancet, (2005), 365, 123-9; Marcellin et al. N. Engl. J. Med., (2004), 351, 1206-17; Buster et al. Hepatology, (2007), 46, 388-94). Therefore, there is an unmet medical need to target HBsAg for HBV treatment (Wieland, S. F. & F. V. Chisari. J Virol, (2005), 79, 9369-80; Kumar et al. J Virol, (2011), 85, 987-95; Woltman et al. PLoS One, (2011), 6, e15324; Op den Brouw et al. Immunology, (2009b), 126, 280-9).
Objects of the present invention are novel compounds of formula I, their manufacture, medicaments based on a compound in accordance with the invention and their production as well as the use of compounds of formula I as HBV inhibitors and for the treatment or prophylaxis of HBV infection. The compounds of formula I show superior anti-HBV activity.
The present invention relates to a compound of formula I
wherein
As used herein, the term “C1-6alkyl” alone or in combination signifies a saturated, linear- or branched chain alkyl group containing 1 to 6, particularly 1 to 4 carbon atoms, for example methyl, ethyl, propyl, isopropyl, 1-butyl, 2-butyl, tert-butyl and the like. Particular “C1-6alkyl” groups are methyl, ethyl, isopropyl and tert-butyl. More particularly, “C1-6alkyl” group is methyl or ethyl.
The term “C3-7cycloalkyl”, alone or in combination, refers to a saturated carbon ring containing from 3 to 7 carbon atoms, particularly from 3 to 6 carbon atoms, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Particular “C3-7cycloalkyl” groups are cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. More particularly, “C3-7cycloalkyl” group is cyclopropyl.
The term “C1-6alkoxy” alone or in combination signifies a group C1-6alkyl-O—, wherein the “C1-6alkyl” is as defined above; for example methoxy, ethoxy, propoxy, iso-propoxy, n-butoxy, iso-butoxy, 2-butoxy, tert-butoxy, pentoxy, hexyloxy and the like. Particular “C1-6alkoxy” groups are methoxy, ethoxy and propoxy. More particularly, “C1-6alkoxy” group is methoxy.
The term “halogen” means fluorine, chlorine, bromine or iodine.
The term “haloC1-6alkyl” denotes a C1-6alkyl group wherein at least one of the hydrogen atoms of the C1-6alkyl group has been replaced by same or different halogen atoms, particularly fluoro atoms. Examples of haloC1-6alkyl include monofluoro-, difluoro- or trifluoro-methyl, -ethyl or -propyl, for example 3,3,3-trifluoropropyl, 3,3-difluoropropyl, 2-fluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, fluoromethyl, difluoromethyl or trifluoromethyl. Particular “haloC1-6alkyl” group is difluoromethyl, 2,2-difluoroethyl or trifluoromethyl. More particularly, “haloC1-6alkyl” group is 2,2-difluoroethyl.
The term “haloC1-6alkoxy” denotes a C1-6alkoxy group wherein at least one of the hydrogen atoms of the C1-6alkoxy group has been replaced by same or different halogen atoms, particularly fluoro atoms. Examples of haloC1-6alkoxyl include monofluoro-, difluoro- or trifluoro-methoxy, -ethoxy or -propoxy, for example fluoropropoxy, difluoropropoxy, trifluoropropoxy, fluoroethoxy, difluoroethoxy, trifluoroethoxy, fluoromethoxy, difluoromethoxy or trifluoromethoxy. Particular “haloC1-6alkoxy” group is 3-fluoropropoxy, 3,3-difluoropropoxy, 3,3,3-trifluoropropoxy, 2-fluoroethoxy, 2,2-difluoroethoxy, 2,2,2-trifluoroethoxy, fluoromethoxy, difluoromethoxy or trifluoromethoxy.
The term “amino” denotes a group of the formula —NR′R″ wherein R′ and R″ are independently hydrogen or C1-6alkyl. Alternatively, R′ and R″, together with the nitrogen to which they are attached, can form a heteroC3-7cycloalkyl.
The term “carbonyl” alone or in combination refers to the group —C(O)—.
The term “cyano” alone or in combination refers to the group —CN.
The term “C1-6alkylsulfonyl” denotes a group —SO2—C1-6alkyl, wherein C1-6alkyl group is defined above. Examples of C1-6alkylsulfonyl include methylsulfonyl and ethylsulfonyl.
The term “enantiomer” denotes two stereoisomers of a compound which are non-superimposable mirror images of one another.
The term “diastereomer” denotes a stereoisomer with two or more centers of chirality and whose molecules are not mirror images of one another. Diastereomers have different physical properties, e.g. melting points, boiling points, spectral properties, and reactivities.
The compounds according to the present invention may exist in the form of their pharmaceutically acceptable salts. The term “pharmaceutically acceptable salt” refers to conventional acid-addition salts or base-addition salts that retain the biological effectiveness and properties of the compounds of formula I and are formed from suitable non-toxic organic or inorganic acids or organic or inorganic bases. Acid-addition salts include for example those derived from inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, sulfamic acid, phosphoric acid and nitric acid, and those derived from organic acids such as p-toluenesulfonic acid, salicylic acid, methanesulfonic acid, oxalic acid, succinic acid, citric acid, malic acid, lactic acid, fumaric acid, and the like. Base-addition salts include those derived from ammonium, potassium, sodium and, quaternary ammonium hydroxides, such as for example, tetramethyl ammonium hydroxide. The chemical modification of a pharmaceutical compound into a salt is a technique well known to pharmaceutical chemists in order to obtain improved physical and chemical stability, hygroscopicity, flowability and solubility of compounds. It is for example described in Bastin R. J., et al., Organic Process Research & Development 2000, 4, 427-435. Particular are the sodium salts of the compounds of formula I.
Compounds of the general formula I which contain one or several chiral centers can either be present as racemates, diastereomeric mixtures, or optically active single isomers. The racemates can be separated according to known methods into the enantiomers. Particularly, diastereomeric salts which can be separated by crystallization are formed from the racemic mixtures by reaction with an optically active acid such as e.g. D- or L-tartaric acid, mandelic acid, malic acid, lactic acid or camphorsulfonic acid.
Inhibitor of HBsAg
The present invention provides (i) a compound having the general formula I:
wherein
A further embodiment of the present invention is (ii) a compound of formula I, wherein,
Another embodiment of the present invention is (iii) a compound of formula I, wherein,
A further embodiment of the present invention is (iv) a compound of formula I, wherein
Another embodiment of the present invention is (v) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R1 is methyl, and all remaining substituents have the significances given herein before.
A further embodiment of the present invention is (vi) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R2 is fluoro, and all remaining substituents have the significances given herein before.
A further embodiment of the present invention is (vii) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R3 and R4 together with the nitrogen to which they are attached form 2-oxa-6-azaspiro[3.3]heptanyl; 2-oxa-7-azaspiro[4.4]nonanyl; azetidinyl substituted with one or two substituents independently selected from C1-6alkoxy, halogen and hydroxyC1-6alkyl; (C1-6alkoxyC1-6alkyl)morpholinyl; (C1-6alkoxyC1-6alkylsulfonyl)piperazinyl; (C1-6alkylsulfonylamino)piperidinyl; or pyrrolidinyl substituted with one or two substituents independently selected from aminocarbonyl, C1-6alkoxy, halogen, hydroxy and hydroxyC1-6alkyl; and all remaining substituents have the significances given herein before.
A further embodiment of the present invention is (viii) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R3 and R4 together with the nitrogen to which they are attached form 2-oxa-6-azaspiro[3.3]heptanyl; 2-oxa-7-azaspiro[4.4]nonanyl; azetidinyl substituted with one or two substituents independently selected from fluoro, hydroxymethyl and methoxy; (methoxymethyl)morpholinyl; (methoxyethylsulfonyl)piperazinyl; (methylsulfonylamino)piperidinyl; or pyrrolidinyl substituted with one or two substituents independently selected from aminocarbonyl, fluoro, hydroxy, hydroxymethyl and methoxy; and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (ix) a compound of formula I, wherein
A further embodiment of the present invention is (x) a compound of formula I, wherein,
Another embodiment of the present invention is (xi) a compound selected from
A further embodiment of the present invention is (xii) a compound selected from
Another embodiment of the present invention is (xiii) a compound of formula I, wherein
A further embodiment of the present invention is (xiv) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R1 is methyl, and all remaining substituents have the significances given herein before.
Another further embodiment of the present invention is (xv) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R2 is fluoro, and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (xvi) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R3 and R4 together with the nitrogen to which they are attached form aminocarbonylpiperidinyl, hydroxypyrrolidinyl or C1-6alkoxypyrrolidinyl, and all remaining substituents have the significances given herein before.
Another further embodiment of the present invention is (xvii) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R3 and R4 together with the nitrogen to which they are attached form aminocarbonylpiperidinyl, hydroxypyrrolidinyl or methoxypyrrolidinyl, and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (xviii) a compound selected from
A further embodiment of the present invention is (xix) a compound which is 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperidine-4-carboxamide; or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof.
Another embodiment of the present invention is (xx) a compound of formula I, wherein
Another embodiment of the present invention is (xxi) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R1 is methyl, and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (xxii) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R2 is fluoro, and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (xxiii) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R3 and R4 together with the nitrogen to which they are attached form azetidinyl; morpholinyl substituted once or twice by C1-6alkyl; C1-6alkyloxopiperazinyl; C1-6alkyloxopyrrolidinyl; piperazinyl substituted with one, two or three substituents independently selected from C1-6alkyl, C1-6alkylcarbonyl and C1-6alkylsulfonyl; or C1-6alkylpyrrolidinyl, and all remaining substituents have the significances given herein before.
A further embodiment of the present invention is (xxiv) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein R3 and R4 together with the nitrogen to which they are attached form azetidinyl; methylmorpholinyl; ethylmorpholinyl; dimethylmorpholinyl; methyloxopiperazinyl; methyloxopyrrolidinyl; piperazinyl substituted with one, two or three substituents independently selected from acetyl, ethylsulfonyl, methyl and methylsulfonyl; or methylpyrrolidinyl, and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (xxv) a compound of formula I, or a pharmaceutically acceptable salt, or enantiomer, or diastereomer thereof, wherein A is N, and all remaining substituents have the significances given herein before.
Another embodiment of the present invention is (xxvi) a compound of formula I, wherein
A further embodiment of the present invention is (xxvii) a compound of formula I, wherein
Another embodiment of the present invention is (xxviii) a compound selected from
Another embodiment of the present invention is (xxix) a compound selected from
The compounds of the present invention can be prepared by any conventional means. Suitable processes for synthesizing these compounds as well as their starting materials are provided in the schemes below and in the examples. All substituents, in particular, A, W, Q and Y are defined as above unless otherwise indicated. Furthermore, and unless explicitly otherwise stated, all reactions, reaction conditions, abbreviations and symbols have the meanings well known to a person of ordinary skill in organic chemistry.
General Synthetic Route for Compound Ia-A, Ia-B and Ia-C (Scheme 1)
The compound of formula Ia-A, Ia-B and Ia-C can be prepared according to Scheme 1, wherein R2 is F, Cl or Br.
Treatment of compound II with DMFDMA in the presence or absence of a suitable solvent such as DMF and CH3CN produces intermediate III. Cyclization of intermediate III with compound IV affords compound V. The reaction can be carried out in the presence of a suitable base such as NaOMe, NaHCO3 or K2CO3 in a suitable solvent such MeOH or EtOH. Deprotection of compound V with an acid such as HCl or TFA generates intermediate VI. Coupling of intermediate VI with halopyridine VII in the presence of a suitable base such as DIPEA in a suitable solvent such as DMSO or NMP gives compound Ia-1 and Ia-2. Compound Ia-A, Ia-B and Ia-C can be obtained by reaction of compound Ia-1 or Ia-2 with amine NHR3R4 in the presence of a suitable base such as K2CO3 or DIPEA in a suitable solvent such as NMP or DMSO, respectively.
General Synthetic Route for Compound Ib (Scheme 2)
The compound of formula Ib can be prepared according to Scheme 2, wherein L1 is Cl, Br or I.
Condensation of intermediate VI with fluoropyridine Ib-1 in the presence of a suitable base such as DIPEA or NaHCO3 in a suitable solvent such as DMSO produces compound Ib-2. Compound Ib can be obtained by coupling of compound Ib-2 with amine NHR3R4 in the presence of a suitable catalyst such as Pd(OAc)2, a suitable ligand such as Xantphos and a suitable base such as Cs2CO3 in a suitable solvent such as dioxane.
This invention also relates to a process for the preparation of a compound of formula I comprising one of the following steps:
(b) coupling of a compound of formula (C)
with a compound of formula (B)
NHR3R4 (B)
A compound of formula I when manufactured according to the above process is also an object of the invention.
Pharmaceutical Compositions and Administration
The invention also relates to a compound of formula I for use as therapeutically active substance.
Another embodiment provides pharmaceutical compositions or medicaments containing the compounds of the invention and a therapeutically inert carrier, diluent or excipient, as well as methods of using the compounds of the invention to prepare such compositions and medicaments. In one example, compounds of formula (I) may be formulated by mixing at ambient temperature at the appropriate pH, and at the desired degree of purity, with physiologically acceptable carriers, i.e., carriers that are non-toxic to recipients at the dosages and concentrations employed into a galenical administration form. The pH of the formulation depends mainly on the particular use and the concentration of compound, but preferably ranges anywhere from about 3 to about 8. In one example, a compound of formula (I) is formulated in an acetate buffer, at pH 5. In another embodiment, the compounds of formula (I) are sterile. The compound may be stored, for example, as a solid or amorphous composition, as a lyophilized formulation or as an aqueous solution.
Compositions are formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. The “effective amount” of the compound to be administered will be governed by such considerations, and is the minimum amount necessary to inhibit HBsAg. For example, such amount may be below the amount that is toxic to normal cells, or the mammal as a whole.
In one example, the pharmaceutically effective amount of the compound of the invention administered parenterally per dose will be in the range of about 0.01 to 100 mg/kg, alternatively about 0.01 to 100 mg/kg of patient body weight per day, with the typical initial range of compound used being 0.3 to 15 mg/kg/day. In another embodiment, oral unit dosage forms, such as tablets and capsules, preferably contain from about 0.1 to about 1000 mg of the compound of the invention.
The compounds of the invention may be administered by any suitable means, including oral, topical (including buccal and sublingual), rectal, vaginal, transdermal, parenteral, subcutaneous, intraperitoneal, intrapulmonary, intradermal, intrathecal and epidural and intranasal, and, if desired for local treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration.
The compounds of the present invention may be administered in any convenient administrative form, e.g., tablets, powders, capsules, solutions, dispersions, suspensions, syrups, sprays, suppositories, gels, emulsions, patches, etc. Such compositions may contain components conventional in pharmaceutical preparations, e.g., diluents, carriers, pH modifiers, sweeteners, bulking agents, and further active agents.
A typical formulation is prepared by mixing a compound of the present invention and a carrier or excipient. Suitable carriers and excipients are well known to those skilled in the art. The formulations may also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents, diluents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).
An example of a suitable oral dosage form is a tablet containing about 0.1 to 1000 mg of the compound of the invention compounded with about 0 to 2000 mg anhydrous lactose, about 0 to 2000 mg sodium croscarmellose, about 0 to 2000 mg polyvinylpyrrolidone (PVP) K30, and about 0 to 2000 mg magnesium stearate. The powdered ingredients are first mixed together and then mixed with a solution of the PVP. The resulting composition can be dried, granulated, mixed with the magnesium stearate and compressed to tablet form using conventional equipment. An example of an aerosol formulation can be prepared by dissolving the compound, for example 0.1 to 1000 mg, of the invention in a suitable buffer solution, e.g. a phosphate buffer, adding a tonicifier, e.g. a salt such sodium chloride, if desired. The solution may be filtered, e.g., using a 0.2 micron filter, to remove impurities and contaminants.
An embodiment, therefore, includes a pharmaceutical composition comprising a compound of Formula I, or a stereoisomer or pharmaceutically acceptable salt thereof. In a further embodiment includes a pharmaceutical composition comprising a compound of Formula I, or a stereoisomer or pharmaceutically acceptable salt thereof, together with a pharmaceutically acceptable carrier or excipient.
The following example A and B illustrate typical compositions of the present invention, but serve merely as representative thereof.
A compound of formula I can be used in a manner known per se as the active ingredient for the production of tablets of the following composition:
A compound of formula I can be used in a manner known per se as the active ingredient for the production of capsules of the following composition:
Indications and Methods of Treatment
The compounds of the invention can inhibit HBsAg production or secretion and inhibit HBV gene expression. Accordingly, the compounds of the invention are useful for the treatment or prophylaxis of HBV infection.
The invention relates to the use of a compound of formula I for the inhibition of HBsAg production or secretion.
The invention relates to the use of a compound of formula I for the inhibition of HBV DNA production.
The invention relates to the use of a compound of formula I for the inhibition of HBV gene expression.
The invention relates to the use of a compound of formula I for the treatment or prophylaxis of HBV infection.
The use of a compound of formula I for the preparation of medicaments useful in the treatment or prophylaxis diseases that are related to HBV infection is an object of the invention.
The invention relates in particular to the use of a compound of formula I for the preparation of a medicament for the treatment or prophylaxis of HBV infection.
Another embodiment includes a method for the treatment or prophylaxis of HBV infection, which method comprises administering an effective amount of a compound of Formula I, a stereoisomer, tautomer, prodrug, conjugates or pharmaceutically acceptable salt thereof.
The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.
Abbreviations Used Herein are as Follows:
μL: microliter
μm: micrometer
μM: micromoles per liter
(Boc)2O: di-tert-butyl dicarbonate
BSA: bovine serum albumin
IC50: the half maximal inhibitory concentration
LC/MS: liquid chromatography/mass spectrometry
M: molarity
MHz: megahertz
min: minute
hr(s): hour(s)
mM: millimoles per liter
MS (ESI): mass spectroscopy (electron spray ionization)
nM: nanomoles per liter
NMR: nuclear magnetic resonance
obsd. observed
rt: room temperature
Pd/C: palladium on activated carbon
Pd(OAc)2 Palladium(II) acetate
TFA: trifluoroacetic acid
δ: chemical shift
Xantphos: 4,5-Bis(diphenylphosphino)-9,9-dimethylxanthene
DMFDMA: N,N-dimethylformamide dimethyl acetal
DIPEA: N,N-diisopropylethylamine
CDI: 1,1′-carbonyldiimidazole
NMP: N-methyl-2-pyrrolidone
DMA: N,N-dimethylacetamide
General Experimental Conditions
Intermediates and final compounds were purified by flash chromatography using one of the following instruments: i) Biotage SP1 system and the Quad 12/25 Cartridge module. ii) ISCO combi-flash chromatography instrument. Silica gel Brand and pore size: i) KP-SIL 60 Å, particle size: 40-60 μm; ii) CAS registry NO: Silica (Gel: 63231-67-4, particle size: 47-60 micron silica gel; iii) ZCX from Qingdao Haiyang Chemical Co., Ltd, pore: 200-300 or 300-400.
Intermediates and final compounds were purified by preparative HPLC on reversed phase column using X Bridge™ Perp C18 (5 μm, OBD™ 30×100 mm) column or SunFire™ Perp C18 (5 μm, OBD™ 30×100 mm) column.
Chiral Separation was conducted on Thar 350 preparative SFC using ChiralPak AD-10u (200×50 mm I.D.) with mobile phase A for CO2 and B for ethanol.
LC/MS spectra were obtained using an Acquity Ultra Performance LC—3100 Mass Detector or Acquity Ultra Performance LC—SQ Detector. Standard LC/MS conditions were as follows (running time 3 minutes):
Acidic condition: A: 0.1% formic acid in H2O; B: 0.1% formic acid in acetonitrile;
Basic condition: A: 0.05% NH3·H2O in H2O; B: acetonitrile;
Neutral condition: A: H2O; B: acetonitrile.
Mass spectra (MS): generally only ions which indicate the parent mass are reported, and unless otherwise stated the mass ion quoted is the positive mass ion (M+H)+.
The microwave assisted reactions were carried out in a Biotage Initiator Sixty or CEM Discover.
NMR Spectra were obtained using Bruker Avance 400 MHz.
Optical rotation was measured on a AUTOPOL® V automatic polarimeter.
All reactions involving air-sensitive reagents were performed under an argon atmosphere.
Reagents were used as received from commercial suppliers without further purification unless otherwise noted.
6-[[2-Fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]hexanoic acid and 6-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]hexanoic acid
To a stirred solution of 4-methoxypyridine (50.0 g, 458 mmol) in anhydrous THF (500 mL) was added a solution of benzyl chloroformate (50.0 g, 458 mmol) in anhydrous THF (400 mL) at −25° C. After being stirred for 1 hr at the same temperature, the reaction mixture was cooled to −40° C. Then to the cooled mixture was added drop-wise methylmagnesium bromide (3.0 M in diethyl ether, 183 mL, 550 mmol). After the addition, the cooling bath was removed. The resulting mixture was stirred at rt for 0.5 hr and then poured into 10% aqueous HCl (1 L). The resulting mixture was stirred further at rt for 10 mins. The reaction was conducted at the same scale for 4 times. The resulting mixtures from 4 batched were combined, and then extracted with EA (2 L) twice. The organic layers were combined and washed sequentially with saturated aqueous NaHCO3 (1 L) and brine (1 L), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with PE/EA=3/1, v:v) to give benzyl 2-methyl-4-oxo-2,3-dihydropyridine-1-carboxylate (420.0 g) as a colorless oil.
To a solution of benzyl 2-methyl-4-oxo-2,3-dihydropyridine-1-carboxylate (100.0 g, 400 mmol) in THF (1 L) was added lithium bis(trimethylsilyl)amide (1.0 M in THF, 960 mL, 960 mmol) drop-wise at −70° C. The resulting mixture was stirred at this temperature for 1 hr. Then to the reaction mixture was added ethyl chloroformate (55.4 g, 440 mmol), and the resulting mixture was stirred further at −70° C. for 3 hrs. The reaction was conducted at the same scale for 4 times. The resulting mixtures from 4 batched were combined, diluted with saturated aqueous NH4Cl (300 mL), and then extracted with EA (3 L). The organic layer was washed sequentially with water (1 L) and brine (1 L), dried over anhydrous Na2SO4 and concentrated in vacuo to give O1-benzyl O3-ethyl 2-methyl-4-oxo-2,3-dihydropyridine-1,3-dicarboxylate (440.0 g, crude) as a yellow oil, which was used in the next step without any further purification.
To a solution of O1-benzyl O3-ethyl 2-methyl-4-oxo-2,3-dihydropyridine-1,3-dicarboxylate (110 g, 347 mmol) in acetic acid (1 L) was added Zn (113 g, 1733 mmol, Alfa) portion-wise. The resulting mixture was heated at 75° C. with stirring for 3 hrs. The reaction was conducted at the same scale for 4 times. The resulting mixtures from 4 batched were combined and filtered. The filtrate was concentrated in vacuo. The residue was diluted with EA (3 L). The resulting mixture was washed sequentially with water (1 L), aqueous NaHCO3 (500 mL) and brine (1 L), and then concentrated in vacuo. The residue was purified by column chromatography (eluting with PE/EA=20/1, v:v) to give O1-benzyl O3-ethyl 2-methyl-4-oxo-piperidine-1,3-dicarboxylate (210 g) as a yellow oil.
To a solution of O1-benzyl O3-ethyl 2-methyl-4-oxo-piperidine-1,3-dicarboxylate (100.0 g, 313 mmol) in 2,2,2-trifluoroethanol (700 mL) was added K2CO3 (129.8 g, 939 mmol) and 2-amidinopyrimidine hydrochloride (54.6 g, 344 mmol). The resulting mixture was heated at 80° C. under N2 for 20 hrs. The reaction was conducted at the same scale twice. The resulting mixtures from 2 batched were combined and filtered. The filtrate was concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give benzyl 4-hydroxy-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (170 g) as a yellow oil.
To a solution of benzyl 4-hydroxy-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (85.0 g, 225 mmol) in MeCN (800 mL) was added POCl3 (172.7 g, 1130 mmol) drop-wise. The mixture was stirred at 60° C. with stirring for 3 hrs. The reaction was conducted at the same scale twice. The resulting mixtures from 2 batched were combined, and poured into ice water (2 L). Then the resulting mixture was stirred for 10 mins and concentrated in vacuo to remove MeCN. The resulting aqueous mixture was basified with saturated aqueous NaHCO3 to pH 7-8, and then extracted with EA (1 L) twice. The combined organic layers were washed with brine (500 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give benzyl 4-chloro-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (150.0 g) as a green oil.
To a solution of benzyl 4-chloro-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (30.0 g, 75.8 mmol) in EtOH (600 mL) was added NH4OH (150 mL), H2O (150 mL) and Pd/C (9.0 g, 10% wt). The mixture was stirred under H2 (20 psi) at 15° C. for 12 hrs. The reaction was conducted at the same scale for 5 times. The resulting mixtures from 5 batched were combined, and filtered. The filtrate was concentrated in vacuo. The residue was dissolved in MeOH (800 mL), and then to the solution was added K2CO3 (83.8 g, 606.6 mmol). The resulting mixture was stirred at 15° C. for 1 hr, and then filtered. The filtrate was concentrate in vacuo to give 5-methyl-2-pyrimidin-2-yl-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine (45.0 g) as a green solid, which was used in the next step without any further purification.
To a mixture of 5-methyl-2-pyrimidin-2-yl-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine (2.0 g, 8.8 mmol) and 2, 4, 6-trifluoropyridine (1.4 g, 10.6 mmol) in NMP (15 mL) was added DIPEA (3.4 g, 26.4 mmol). Then the reaction vessel was sealed and heated under microwave at 150° C. for 1 hr. The reaction was conducted at the same scale for 10 times. The resulting mixtures from 10 batched were combined and diluted with EA (600 mL). The resulting mixture was washed sequentially with water (200 mL) and brine (200 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) and prep-HPLC to give 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (3.5 g) and 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (1.0 g).
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (100 mg, 294 μmol), 6-aminohexanoic acid (57.8 mg, 441 μmol) and potassium carbonate (81.2 mg, 588 μmol) in DMSO (2 mL) was heated at 120° C. in a microwave reactor for 1 hr. The mixture was cooled to rt and purified by prep-HPLC to give 6-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]hexanoic acid (40 mg) and 6-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]hexanoic acid (10 mg) as light brown solids.
6-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]hexanoic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 1.46 (br s, 2H), 1.55 (d, 3H), 1.59-1.72 (m, 4H), 2.14-2.42 (m, 3H), 3.07-3.31 (m, 3H), 3.38-3.51 (m, 1H), 4.33-4.46 (m, 1H), 5.53 (d, 1H), 5.65-5.88 (m, 1H), 7.62-7.70 (m, 1H), 8.20 (s, 1H), 8.85 (s, 1H), 9.02 (d, 2H). MS obsd. (ESI+) [(M+H)+]: 452.
6-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]hexanoic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 1.40-1.71 (m, 9H), 2.28-2.37 (m, 2H), 3.05-3.24 (m, 4H), 3.40-3.56 (m, 1H), 4.51 (br dd, 1H), 5.54 (d, 1H), 5.68-5.90 (m, 1H), 7.61-7.66 (m, 1H), 8.11 (br s, 1H), 8.89 (s, 1H), 9.04 (br d, 2H). MS obsd. (ESI+) [(M+H)+]: 452.
To a stirred solution of 2-hydroxyacetamide (26.5 mg, 0.35 mmol) in DMF (1 mL) was added NaH (28 mg, 0.70 mmol) and 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol) at 0° C. successively. After being heated at 80° C. for 1 hr, the resulting mixture was partitioned between EA (150 mL) and brine (50 mL). The separated organic layer was dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-hydroxy-acetamide (24 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 10.10 (s, 1H), 8.99 (d, 2H), 8.95 (s, 1H), 7.63 (t, 1H), 7.17 (s, 1H), 6.82 (s, 1H), 5.56 (m, 1 H), 4.32 (d, 1H), 4.02 (s, 2H), 3.47-3.54 (m, 1H), 2.97-3.11 (m, 2H), 1.47-1.57 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 396.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80.0 mg, 0.235 mmol), 2-methylmorpholine (71.3 mg, 0.705 mmol) and K2CO3 (97.4 mg, 0.705 mmol) in DMA (3 mL) was heated at 110° C. for 15 hrs. The resulting mixture was poured into brine (4 mL) and then extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-methyl-morpholine (12.7 mg) as a yellow solid and 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-methyl-morpholine (11.0 mg) as a yellow solid.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-methyl-morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 8.98-9.06 (m, 2H), 8.81 (s, 1H), 7.43 (t, 1H), 5.82 (s, 1H), 5.63-5.74 (m, 2H), 4.33-4.42 (m, 1H), 3.97-4.05 (m, 1H), 3.72 (br. s., 2H), 3.42-3.59 (m, 3H), 3.20-3.30 (m, 2H), 2.97 (d, 1H), 2.63 (d, 1H), 1.56 (d, 3H), 1.27 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-methyl-morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.03 (d, 2H), 8.81 (s, 1H), 7.44 (t, 1H), 5.79-5.88 (m, 1H), 5.72 (d, 1H), 5.56-5.65 (m, 1H), 4.37-4.50 (m, 1H), 4.01 (br. s., 3H), 3.69 (dd, 2H), 3.40-3.51 (m, 1H), 3.19-3.32 (m, 2H), 2.95 (s, 1H), 2.61 (s, 1H), 1.57 (d, 3H), 1.28 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80.0 mg, 0.235 mmol), 2-ethylmorpholine (81.2 mg, 0.705 mmol) and K2CO3 (97.4 mg, 0.705 mmol) in DMA (3 mL) was heated at 110° C. for 15 hrs, and then poured into brine (4 mL). The resulting mixture was extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water then brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 2-ethyl-4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholine (23.7 mg) as a light yellow solid and 2-ethyl-4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholine (10.6 mg) as a yellow solid.
2-ethyl-4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.02 (d, 2H), 8.81 (s, 1H), 7.43 (t, 1H), 5.82 (s, 1H), 5.65-5.74 (m, 2H), 4.30-4.44 (m, 1H), 3.98-4.08 (m, 1H), 3.64-3.77 (m, 1H), 3.38-3.59 (m, 4H), 3.17-3.35 (m, 2H), 2.92-3.04 (m, 1H), 2.58-2.69 (m, 1H), 1.56 (m, 5H), 1.03 (t, 3H). MS obsd. (ESI+) [(M+H)+]: 436.
2-ethyl-4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.04 (d, 2H), 8.80 (s, 1H), 7.41-7.46 (m, 1H), 5.80-5.87 (m, 1H), 5.69-5.75 (m, 1H), 5.58-5.67 (m, 1H), 4.36-4.47 (m, 1H), 3.89-4.09 (m, 3H), 3.64-3.75 (m, 1H), 3.39-3.52 (m, 2H), 3.18-3.29 (m, 2H), 2.91-3.03 (m, 1H), 2.58-2.68 (m, 1H), 1.61-1.70 (m, 2H), 1.57 (d, 3H), 1.04 (t, 3H). MS obsd. (ESI+) [(M+H)+]: 436.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80.0 mg, 0.235 mmol), 2,2-dimethylmorpholine (81.2 mg, 0.705 mmol) and K2CO3 (97.4 mg, 0.705 mmol) in DMA (3 mL) was heated at 110° C. for 15 hrs, and at 130° C. for 15 hrs. The resulting mixture was poured into brine (4 mL), and extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2,2-dimethyl-morpholine (21.5 mg) as a light yellow solid and 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2,2-dimethyl-morpholine (11.2 mg) as a yellow solid.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2,2-dimethyl-morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.00-9.05 (m, 2H), 8.81 (s, 1H), 7.41-7.45 (m, 1H), 5.76-5.80 (m, 1H), 5.68-5.73 (m, 1H), 5.67 (s, 1H), 4.32-4.42 (m, 1H), 3.81-3.90 (m, 2H), 3.41-3.53 (m, 1H), 3.19-3.33 (m, 4H), 3.11 (s, 2H), 1.53-1.59 (m, 3H), 1.30 (s, 6H). MS obsd. (ESI+) [(M+H)+]: 436.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2,2-dimethyl-morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.03 (d, 2H), 8.81 (s, 1H), 7.41-7.46 (m, 1H), 5.77-5.83 (m, 1H), 5.65-5.72 (m, 1H), 5.56-5.64 (m, 1H), 4.37-4.48 (m, 1H), 3.79-3.90 (m, 2H), 3.39-3.61 (m, 3H), 3.17-3.36 (m, 4H), 1.57 (d, 3H), 1.30 (s, 6H). MS obsd. (ESI+) [(M+H)+]: 436.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.3 mmol), 3-oxa-8-azabicyclo[3.2.1]octane hydrochloride (132 mmol) and K2CO3 (122 mg, 0.9 mmol) in DMA (1 mL) was heated at 110° C. with stirring for 12 hrs. The resulting mixture was cooled to rt and concentrated in vacuo. The residue was partitioned between DCM (10 mL) and brine (5 mL). The organic layer was concentrated in vacuo. The residue was purified by prep-HPLC to give 8-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-oxa-8-azabicyclo[3.2.1]octane (7.8 mg) as a white solid and 8-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-3-oxa-8-azabicyclo[3.2.1]octane (3.1 mg) as a white solid.
8-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-oxa-8-azabicyclo[3.2.1]octane, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (s, 1H), 7.45 (t, 1H), 5.77-5.67 (m, 2H), 5.62 (s, 1H), 4.46-4.35 (m, 1H), 4.11 (br. s., 2H), 3.87 (d, 2H), 3.58 (d, 2H), 3.54-3.43 (m, 1H), 3.37-3.19 (m, 2H), 2.19-2.03 (m, 4H), 1.60 (br. s., 3H) MS obsd. (ESI+) [(M+H)+]: 434.
8-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-3-oxa-8-azabicyclo[3.2.1]octane, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.82 (s, 1H), 7.45 (t, 1H), 5.82 (d, 1H), 5.69 (d, 1H), 5.65 (d, 1H), 4.46-4.33 (m, 3H), 3.92-3.83 (m, 2H), 3.66-3.60 (m, 2H), 3.53-3.42 (m, 1H), 3.33-3.22 (m, 2H), 2.15-1.96 (m, 4H), 1.59 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 434.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.3 mmol), 2-oxa-7-azaspiro[4.4]nonane (112 mmol) and K2CO3 (122 mg, 0.9 mmol) in DMA (1 mL) was heated at 110° C. with stirring for 12 hrs. The resulting mixture was cooled to rt and concentrated in vacuo. The residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo. The residue was purified by prep-HPLC to give 7-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-oxa-7-azaspiro[4.4]nonane (31.2 mg) as a white solid and 7-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-oxa-7-azaspiro[4.4]nonane (17.9 mg) as a white solid.
7-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-oxa-7-azaspiro[4.4]nonane, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (s, 1H), 7.45 (t, 1H), 5.72 (d, 1H), 5.54 (s, 1H), 5.48 (s, 1H), 4.49-4.33 (m, 1H), 3.98 (t, 2H), 3.79-3.66 (m, 2H), 3.55-3.16 (m, 7H), 2.19-1.88 (m, 4H), 1.59 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 436.
7-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-oxa-7-azaspiro[4.4]nonane, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (s, 1H), 7.45 (t, 1H), 5.77 (d, 1H), 5.74-5.66 (m, 1H), 5.48 (d, 1H), 4.52-4.41 (m, 1H), 3.98 (t, 2H), 3.82-3.65 (m, 2H), 3.62-3.39 (m, 5H), 3.35-3.18 (m, 2H), 2.14-1.89 (m, 4H), 1.60 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 436.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100.0 mg, 0.29 mmol), 2-(methoxymethyl)morpholine hydrochloride (147.5 mg, 0.88 mmol) and K2CO3 (121.6 mg, 0.88 mmol) in DMA (3 mL) was heated at 110° C. with stirring for 48 hrs. The resulting mixture was poured into brine (4 mL) and extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-(methoxymethyl)morpholine (29 mg) as a white solid and 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-(methoxymethyl)morpholine (15 mg) as a white solid.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-(methoxymethyl)morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.03 (d, 2H), 8.82 (s, 1H), 7.43 (s, 1H), 5.82-5.86 (m, 1H), 5.67-5.74 (m, 2H), 4.33-4.44 (m, 1H), 4.02-4.11 (m, 1H), 3.71-3.83 (m, 2H), 3.58-3.66 (m, 1H), 3.46-3.58 (m, 4H), 3.44 (s, 3H), 3.20-3.34 (m, 2H), 2.96-3.08 (m, 1H), 2.77-2.87 (m, 1H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 452.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-(methoxymethyl)morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.02 (d, 2H), 8.80 (s, 1H), 7.43 (t, 1H), 5.81-5.87 (m, 1H), 5.69-5.76 (m, 1H), 5.56-5.64 (m, 1H), 4.35-4.48 (m, 1H), 3.89-4.12 (m, 3H), 3.66-3.80 (m, 2H), 3.48-3.58 (m, 2H), 3.43 (s, 4H), 3.20-3.27 (m, 2H), 2.93-3.03 (m, 1H), 2.77 (s, 1H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 452.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100.0 mg, 0.29 mmol), 1-(piperazin-1-yl)ethanone (112.7 mg, 0.88 mmol) and K2CO3 (121.6 mg, 0.88 mmol) in DMA (3 mL) was heated at 110° C. with stirring for 15 hrs. The resulting mixture was poured into brine (4 mL) and extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 1-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]ethanone (26 mg) as a yellow solid and 1-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]ethanone (12 mg) as a yellow solid.
1-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]ethanone, 1H NMR (400 MHz CDCl3) δ ppm: 9.02 (d, 2H), 8.81 (s, 1H), 7.43 (s, 1H), 5.82 (s, 1H), 5.65-5.74 (m, 2H), 4.30-4.44 (m, 1H), 3.73-3.82 (m, 2H), 3.62 (d, 2H), 3.46-3.53 (m, 1H), 3.37 (dt, 4H), 3.25 (dd, 2H), 2.15 (s, 3H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 449.
1-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]ethanone, 1H NMR (400 MHz CDCl3) δ ppm: 9.02 (d, 2H), 8.80 (s, 1H), 7.40-7.45 (m, 1H), 5.81-5.89 (m, 1H), 5.70-5.76 (m, 1H), 5.57-5.66 (m, 1H), 4.35-4.47 (m, 1H), 3.70-3.78 (m, 2H), 3.60 (s, 4H), 3.49 (d, 3H), 3.19-3.31 (m, 2H), 2.15 (s, 3H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 449.
To a solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol) and 3-methylpyrrolidine hydrochloride (86 mg, 0.70 mmol) in DMA (1 mL) was added K2CO3 (97 mg, 0.70 mmol). The mixture was heated at 110° C. with stirring for 12 hrs and then cooled to rt. The mixture was concentrated in vacuo and the residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[6-fluoro-4-(3-methylpyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (18 mg) as a white solid and 6-[4-fluoro-6-(3-methylpyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (9.8 mg) as a white solid.
6-[6-fluoro-4-(3-methylpyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (br. s., 1H), 7.45 (t, 1H), 5.73 (d, 1H), 5.53 (s, 1H), 5.47 (s, 1H), 4.42 (d, 1H), 3.56-3.16 (m, 8H), 2.92 (t, 1H), 2.43 (dd, 1H), 2.23-2.10 (m, 1H), 1.73-1.61 (m, 2H), 1.16 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 406.
6-[4-fluoro-6-(3-methylpyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 1H), 8.82 (s, 1H), 7.44 (t, 1H), 5.73 (d, 2H), 5.47 (d, 1H), 4.47 (d, 1H), 3.72-3.16 (m, 7H), 2.99 (t, 1H), 2.49-2.31 (m, 1H), 2.20-2.07 (m, 1H), 1.68-1.62 (m, 1H), 1.58 (s, 2H), 1.16 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 406.
To a solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol) and 3-methoxypyrrolidine hydrochloride (96 mg, 0.70 mmol) in DMA (1 mL) was added K2CO3 (97 mg, 0.70 mmol). The mixture was heated at 110° C. with stirring for 12 hrs and then cooled to rt. The mixture was concentrated in vacuo and the residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[6-fluoro-4-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (34.0 mg) as a white solid and 6-[4-fluoro-6-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (5.4 mg) as a white solid.
6-[6-fluoro-4-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (s, 1H), 7.44 (t, 1H), 5.81-5.65 (m, 1H), 5.55 (s, 1H), 5.49 (s, 1H), 4.48-4.34 (m, 1H), 4.12 (d, 1H), 3.57-3.37 (m, 9H), 3.35-3.17 (m, 2H), 2.27-2.04 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
6-[4-fluoro-6-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.96 (d, 2H), 8.72 (s, 1H), 7.35 (t, 1H), 5.73-5.59 (m, 2H), 5.40 (d, 1H), 4.37 (dd, 1H), 4.07-3.94 (m, 1H), 3.57-3.35 (m, 5H), 3.34-3.28 (m, 3H), 3.24-3.09 (m, 2H), 2.16-1.91 (m, 2H), 1.49 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
To a solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol) and 3,3-difluoropyrrolidine hydrochloride (100 mg, 0.70 mmol) in DMA (1 mL) was added K2CO3 (97 mg, 0.70 mmol). The mixture was heated at 110° C. with stirring for 12 hrs and then cooled to rt. The mixture was concentrated in vacuo and the residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[4-(3,3-difluoropyrrolidin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (13.6 mg) as a white solid and 6-[6-(3,3-difluoropyrrolidin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (7.2 mg) as a white solid.
6-[4-(3,3-difluoropyrrolidin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.96 (d, 1H), 8.74 (s, 1H), 7.36 (t, 1H), 5.63 (q, 1H), 5.44 (s, 1H), 5.39 (s, 1H), 4.32 (d, 1H), 3.65 (t, 2H), 3.52 (t, 2H), 3.46-3.34 (m, 2H), 3.23-3.14 (m, 2H), 2.52-2.37 (m, 2H), 1.51 (s, 3H). MS obsd. (ESI+) [(M+H)+]: 428.
6-[6-(3,3-difluoropyrrolidin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.96 (d, 2H), 8.74 (s, 1H), 7.36 (t, 1H), 5.75 (d, 1H), 5.60 (q, 1H), 5.41 (d, 1H), 4.42-4.29 (m, 1H), 3.78 (t, 2H), 3.58 (t, 2H), 3.44-3.31 (m, 1H), 3.22-3.12 (m, 2H), 2.41 (tt, 2H), 1.51 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 428.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), morpholin-2-ylmethanol hydrochloride (68 mg, 0.44 mmol) and K2CO3 (122 mg, 0.88 mmol) in DMA (2 mL) was heated at 110° C. with stirring for 12 hrs. Then the mixture was cooled and partitioned between H2O (5 mL) and EA (20 mL). The aqueous layer was extracted with EA (20 mL) twice. The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give [4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholin-2-yl]methanol (12 mg) as a yellow solid and [4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholin-2-yl]methanol (10 mg) as a light yellow solid.
[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholin-2-yl]methanol, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.65 (t, 1H), 6.10 (s, 1H), 5.84 (s, 1H), 5.73 (q, 1H), 4.46-4.56 (m, 1H), 4.02 (dd, 1H), 3.61-3.84 (m, 6H), 3.43-3.56 (m, 1H), 3.06-3.25 (m, 2H), 2.87-3.00 (m, 1H), 2.64-2.76 (m, 1H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 438.
[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholin-2-yl]methanol, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.86-8.93 (m, 1H), 7.65 (t, 1H), 6.03 (d, 1H), 5.85 (d, 1H), 5.75 (q, 1H), 4.52 (d, 1H), 3.96-4.22 (m, 3H), 3.55-3.73 (m, 4H), 3.43-3.53 (m, 1H), 3.11-3.19 (m, 2H), 2.91 (tt, 1H), 2.60-2.73 (m, 1H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 438.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 200 mg, 0.59 mmol), morpholine-2-carboxylic acid hydrochloride (295 mg, 1.76 mmol) and K2CO3 (406 mg, 2.94 mmol) in NMP (5 mL) was heated at 140° C. with stirring for 12 hrs. The mixture was cooled to rt and diluted with H2O (10 mL). The resulting mixture was extracted with EA (30 mL) for three times. The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholine-2-carboxylic acid (35 mg) as a yellow solid and 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholine-2-carboxylic acid (22 mg) as a light yellow solid.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholine-2-carboxylic acid, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.91 (s, 1H), 7.63 (t, 1H), 6.13 (s, 1H), 5.89 (s, 1H), 5.70 (q, 1H), 4.47 (dd, 1H), 4.15 (d, 1H), 3.99 (d, 1H), 3.54-3.77 (m, 4H), 3.10-3.20 (m, 2H), 2.95-3.06 (m, 2H), 1.48 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 452.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholine-2-carboxylic acid, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (s, 1H), 8.87 (s, 1H), 7.64 (s, 1H), 6.15 (d, 1H), 5.96 (d, 1H), 5.67 (s, 1H) 4.50 (m, 1H), 3.94-4.22 (m, 3H), 3.55-3.81 (m, 3H), 3.01 (m, 4H), 1.50 (br. s., 3H). MS obsd. (ESI+)[(M+H)+]: 452.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 2-(morpholin-2-yl)acetic acid hydrochloride (160 mg, 0.88 mmol) and K2CO3 (203 mg, 1.47 mmol) in NMP (3 mL) was heated at 140° C. with stirring for 12 hrs. The mixture was cooled to rt and diluted with H2O (5 mL). The resulting mixture was extracted with EA (20 mL) for three times. The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 2-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholin-2-yl]acetic acid (31 mg) as a light yellow solid and 2-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholin-2-yl]acetic acid (13 mg) as a light yellow solid.
2-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholin-2-yl]acetic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 6.10 (s, 1H), 5.83 (s, 1H), 5.73 (q, 1H), 4.44-4.56 (m, 1H), 3.96 (dd, 2H), 3.85 (d, 1H), 3.64-3.75 (m, 2H), 3.42-3.54 (m, 1H), 3.07-3.25 (m, 2H), 2.90-3.02 (m, 1H), 2.64-2.75 (m, 1H), 2.47-2.61 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 466.
2-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholin-2-yl]acetic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.64 (s, 1H), 6.03 (d, 1H), 5.84 (d, 1H), 5.72-5.80 (m, 1H), 4.47 (t, 1H), 4.17-4.35 (m, 1H), 3.85-4.06 (m, 3H), 3.60-3.72 (m, 1H), 3.44-3.58 (m, 1H), 3.13 (m, 2H), 2.93 (t, 1H), 2.64 (t, 1H), 2.55 (m, 1H), 1.58 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 466.
To a solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol) and pyrrolidin-2-ylmethanol (90 mg, 0.70 mmol) in DMA (1 mL) was added K2CO3 (97 mg, 0.70 mmol). The mixture was heated at 110° C. with stirring for 12 hrs. The resulting mixture was then cooled to rt and concentrated in vacuo. The residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give [1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidin-2-yl]methanol (36.3 mg) as a white solid and [1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidin-2-yl]methanol (3.2 mg) as a white solid.
[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidin-2-yl]methanol, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (s, 1H), 7.45 (t, 1H), 5.78-5.65 (m, 2H), 5.60 (s, 1H), 4.41 (d, 1H), 3.94 (br. s., 1H), 3.79-3.61 (m, 2H), 3.56-3.40 (m, 2H), 3.37-3.20 (m, 3H), 2.18-1.97 (m, 4H), 1.57 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 422.
[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidin-2-yl]methanol, 1H NMR (400 MHz, CDCl3) δ ppm: 8.96 (d, 2H), 8.75 (s, 1H), 7.36 (t, 1H), 5.71 (d, 1H), 5.61 (d, 1H), 5.47 (d, 1H), 4.39 (br. s., 1H), 4.23 (d, 1H), 3.73 (dd, 1H), 3.65-3.56 (m, 1H), 3.50-3.32 (m, 2H), 3.26-3.05 (m, 3H), 2.08-1.80 (m, 3H), 1.71 (d, 1H), 1.49-1.43 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), methyl pyrrolidine-2-carboxylate hydrochloride (116 mg, 0.70 mmol) and K2CO3 (97 mg, 0.70 mmol) in DMA (1 mL) was heated at 110° C. with stirring for 12 hrs. The mixture was cooled to rt and concentrated in vacuo. The residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was dissolved in a mixture of H2O and MeOH (1 mL/1 mL). To the resulting solution was added NaOH (92 mg). The resulting mixture was heated at 50° C. with stirring for 5 hrs. After being cooled to rt, the mixture was concentrated in vacuo. The residue was dissolved in H2O (3 mL) and the resulting solution was acidified to pH=6 using 1M HCl. The mixture was extracted with DCM (5 mL) for 5 times. The combined organic layers were concentrated in vacuo and the residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-2-carboxylic acid (30.4 mg) as a white solid and 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-2-carboxylic acid (12.4 mg) as a white solid.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-2-carboxylic acid, 1H NMR (400 MHz, CDCl3) δ ppm: 8.94-8.90 (t, 2H), 8.70 (d, 1H), 7.33 (t, 1H), 5.60 (m, 1H), 5.51 (d, 1H), 5.41 (s, 1H), 4.34-4.20 (m, 2H), 3.43-3.10 (m, 5H), 1.45 (m.3H). MS obsd. (ESI+) [(M+H)+]: 436.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-2-carboxylic acid, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (m, 2H), 8.82 (m, 1H), 7.45 (m, 1H), 5.85 (m, 1H), 5.57-5.40 (m, 2H), 4.62 (s, 1H), 4.27 (m, 1H), 3.70-3.10 (m, 5H), 2.09 (s, 2H), 1.55 (m.3H). MS obsd. (ESI+) [(M+H)+]: 436.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), 2-(methoxymethyl)-pyrrolidine (80 mg, 0.70 mmol) and K2CO3 (97 mg, 0.70 mmol) in DMA (1 mL) was heated at 120° C. with stirring for 12 hrs. After being cooled to rt, the mixture was concentrated in vacuo. The residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[6-fluoro-4-[2-(methoxymethyl)pyrrolidin-1-yl]-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (21.7 mg) as a white solid and 6-[4-fluoro-6-[2-(methoxymethyl)pyrrolidin-1-yl]-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (12.8 mg) as a white solid.
6-[6-fluoro-4-[2-(methoxymethyl)pyrrolidin-1-yl]-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 9.04 (d, 2H), 8.82 (s, 1H), 7.44 (t, 1H), 5.78-5.64 (m, 2H), 5.58 (s, 1H), 4.48-4.34 (m, 1H), 3.95 (d, 1H), 3.54-3.43 (m, 3H), 3.40 (d, 3H), 3.37-3.18 (m, 4H), 2.16-1.95 (m, 4H), 1.58 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 436.
6-[4-fluoro-6-[2-(methoxymethyl)pyrrolidin-1-yl]-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.80 (d, 1H), 7.48-7.42 (m, 1H), 5.83-5.69 (m, 2H), 5.54 (d, 1H), 4.49-4.36 (m, 1H), 4.28 (br. s., 1H), 3.69 (ddd, 16.3 Hz, 1H), 3.53-3.45 (m, 2H), 3.43 (d, 3H), 3.34-3.17 (m, 4H), 2.15-1.89 (m, 4H), 1.59 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 436.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), pyrrolidin-3-ol (61 mg, 0.70 mmol) and K2CO3 (97 mg, 0.70 mmol) in DMA (1 mL) was heated at 120° C. with stirring for 12 hrs and then cooled to rt. The mixture was concentrated in vacuo and the residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidin-3-ol (23.6 mg) as a white solid and 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidin-3-ol (10.2 mg) as a white solid.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidin-3-ol, 1H NMR (400 MHz, CDCl3) δ ppm: 9.04 (d, 2H), 8.83 (s, 1H), 7.45 (t, 1H), 5.78-5.66 (m, 1H), 5.56 (s, 1H), 5.50 (s, 1H), 4.66 (br. s., 1H), 4.49-4.35 (m, 1H), 3.66-3.41 (m, 4H), 3.39-3.19 (m, 3H), 2.28-2.07 (m, 2H), 1.75 (d, 1H), 1.58 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 408.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidin-3-ol, 1H NMR (400 MHz, CDCl3) δ 9.05 (d, 2H), 8.82 (s, 1H), 7.45 (t, 1H), 5.82-5.68 (m, 2H), 5.51 (d, 1H), 4.63 (br. s., 1H), 4.46 (d, 1H), 3.72-3.39 (m, 5H), 3.33-3.18 (m, 2H), 2.25-2.01 (m, 2H), 1.65 (d, 1H), 1.58 (br. s., 3H). MS obsd. (ESI+)[(M+H)+]: 408.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), methyl pyrrolidine-3-carboxylate (90 mg, 0.70 mmol) and K2CO3 (97 mg, 0.70 mmol) in DMA (1 mL) was heated at 120° C. with stirring for 12 hrs and then cooled to rt. The mixture was concentrated in vacuo and the residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated to give a solid (100 mg). Then the obtained solid (50 mg) was dissolved in H2O (1 mL) and MeOH (1 mL), followed by addition of NaOH (22 mg, 0.55 mmol). The resulting mixture was heated at 50° C. with stirring for 12 hrs, and then concentrated in vacuo. The residue was dissolved in H2O (2 mL) and acidified to pH=6 using 1M HCl. The resulting mixture was extracted with DCM (5 mL) for 4 times. The combined organic layers were concentrated in vacuo and the residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-3-carboxylic acid (12.8 mg) as a white solid and 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-3-carboxylic acid (9.9 mg) as a white solid.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-3-carboxylic acid, 1H NMR (400 MHz, CDCl3) δ ppm: 8.96 (d, 2H), 8.75 (d, 1H), 7.37 (t, 1H), 5.61 (q, 1H), 5.48 (s, 1H), 5.41 (s, 1H), 4.35 (d, 1H), 3.66-3.50 (m, 2H), 3.48-3.29 (m, 3H), 3.26-3.09 (m, 3H), 2.28 (q, 2H), 1.48 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 436.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-3-carboxylic acid, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.84 (s, 1H), 7.45 (t, 1H), 5.77 (d, 2H), 5.49 (d, 1H), 4.43 (d, 1H), 3.77 (br. s., 2H), 3.60 (br. s., 1H), 3.47 (br. s., 2H), 3.24 (br. s., 3H), 2.32 (br. s., 2H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 436.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80.0 mg, 0.235 mmol), 3-methylmorpholine (71.3 mg, 0.705 mmol) and DIPEA (91.1 mg, 0.705 mmol) in NMP (3 mL) was heated at 140° C. for 32 hrs. The mixture was poured into brine (4 mL), and the resulting mixture was extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-methyl-morpholine (12 mg) as a white solid and 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-3-methyl-morpholine (10 mg) as a yellow solid.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-methyl-morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 8.99-9.06 (m, 2H), 8.81 (s, 1H), 7.40-7.46 (m, 1H), 5.75-5.81 (m, 1H), 5.64-5.73 (m, 2H), 4.33-4.44 (m, 1H), 3.99-4.06 (m, 1H), 3.75-3.90 (m, 3H), 3.59-3.70 (m, 1H), 3.42-3.53 (m, 1H), 3.18-3.35 (m, 4H), 1.55-1.59 (m, 3H), 1.21-1.29 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-3-methyl-morpholine, 1H NMR (400 MHz CDCl3) δ ppm: 9.03 (d, 2H), 8.77-8.84 (m, 1H), 7.40-7.46 (m, 1H), 5.79-5.85 (m, 1H), 5.56-5.70 (m, 2H), 4.33-4.49 (m, 1H), 4.13-4.33 (m, 1H), 3.99-4.07 (m, 1H), 3.69-3.87 (m, 3H), 3.57-3.66 (m, 1H), 3.40-3.51 (m, 1H), 3.15-3.33 (m, 3H), 1.55-1.59 (m, 3H), 1.22-1.29 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
A mixture of methyl pyrrolidine-2-carboxylate hydrochloride (500 mg, 3.01 mmol) in a solution of NH3 in THF (10 mL) was heated at 50° C. with stirring for 12 hrs. The mixture was concentrated in vacuo to give pyrrolidine-2-carboxamide (450 mg, crude) as a white solid, which was used directly in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), pyrrolidine-2-carboxamide (105 mg, 0.70 mmol) and K2CO3 (97 mg, 0.70 mmol) in DMA (1 mL) was heated at 120° C. with stirring for 12 hrs. After being cooled to rt, the mixture was concentrated in vacuo. The residue was partitioned between DCM (10 mL) and H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-2-carboxamide (11.3 mg) as a brown solid and 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-2-carboxamide (5.7 mg) as a brown solid.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-2-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 9.03 (d, 2H), 8.82 (s, 1H), 7.41-7.46 (m, 1H), 6.03-6.13 (m, 1H), 5.63-5.73 (m, 1H), 5.61 (s, 1H), 5.56 (s, 1H), 5.35-5.46 (m, 1H), 4.33-4.44 (m, 1H), 4.10-4.20 (m, 1H), 3.61-3.72 (m, 1H), 3.32-3.52 (m, 2H), 3.19-3.30 (m, 2H), 2.26-2.41 (m, 2H), 1.99-2.18 (m, 2H), 1.52-1.57 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-2-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.83 (s, 1H), 7.45 (t, 1H), 6.40-6.19 (m, 1H), 5.87 (d, 1H), 5.75-5.66 (m, 1H), 5.63 (d, 1H), 5.35-5.15 (m, 1H), 4.54 (t, 1H), 4.43-4.26 (m, 1H), 3.69-3.55 (m, 1H), 3.54-3.43 (m, 1H), 3.36 (q, 1H), 3.29-3.20 (m, 2H), 2.41-2.16 (m, 2H), 2.15-2.04 (m, 2H), 1.58 (br. s., 3H). MS obsd. (ESI+) [(M+H)+]: 435.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80.0 mg, 0.235 mmol), pyrrolidine-3-carboxamide (80.5 mg, 0.705 mmol) and K2CO3 (97.5 mg, 0.705 mmol) in DMA (3 mL) was heated at 130° C. with stirring for 24 hrs. The mixture was diluted with brine (4 mL) and extracted with DCM (30 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-3-carboxamide (19.0 mg) as a yellow solid and 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-3-carboxamide (6.4 mg) as a white solid.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidine-3-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 9.03 (d, 2H), 8.81 (s, 1H), 7.43 (s, 1H), 5.54 (s, 4H), 5.47 (s, 1H), 4.35-4.46 (m, 1H), 3.57-3.64 (m, 2H), 3.50-3.57 (m, 1H), 3.35-3.49 (m, 2H), 3.18-3.33 (m, 2H), 3.06-3.15 (m, 1H), 2.25-2.37 (m, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]pyrrolidine-3-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 9.03 (d, 2H), 8.81 (s, 1H), 7.41-7.46 (m, 1H), 5.74-5.80 (m, 1H), 5.58-5.74 (m, 2H), 5.49 (dd, 2H), 4.37-4.50 (m, 1H), 3.74-3.82 (m, 1H), 3.61-3.73 (m, 2H), 3.39-3.51 (m, 2H), 3.17-3.31 (m, 2H), 3.03-3.13 (m, 1H), 2.25-2.34 (m, 2H), 1.54-1.59 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
A mixture of 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholine-2-carboxylic acid (Example 26, 60 mg, 0.13 mmol), NH4Cl (71 mg, 1.33 mmol), HATU (252 mg, 0.66 mmol) and DIPEA (86 mg, 0.66 mmol) in DMSO (3 mL) was heated at 90° C. with stirring for 12 hrs. The mixture was cooled to rt and diluted with H2O (5 mL). The resulting mixture was extracted with EA (10 mL) for three times.
The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]morpholine-2-carboxamide (31 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.64 (t, 1H), 6.12 (s, 1H), 5.86 (s, 1H), 5.74 (q, 1H), 4.45-4.58 (m, 1H), 4.00-4.15 (m, 3H), 3.67-3.84 (m, 2H), 3.43-3.56 (m, 1H), 3.09-3.20 (m, 2H), 3.01 (td, 1H), 2.82-2.92 (m, 1H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 451.
A mixture of 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholine-2-carboxylic acid (Example 27, 10 mg, 0.02 mmol), NH4Cl (12 mg, 0.22 mmol), HATU (42 mg, 0.11 mmol) and DIPEA (29 mg, 0.22 mmol) in DMSO (1 mL) was heated at 90° C. with stirring for 12 hrs. The mixture was then cooled to rt and diluted with H2O (2 mL). The resulting mixture was extracted with EA (5 mL) for three times. The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]morpholine-2-carboxamide (4 mg) as a white solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.92 (s, 1H), 7.65 (t, 1H), 6.06 (d, 1H), 5.88 (d, 1H), 5.79 (t, 1H), 4.43-4.58 (m, 2H), 3.94-4.18 (m, 3H), 3.73 (td, 1H), 3.43-3.57 (m, 1H), 3.09-3.20 (m, 2H), 2.98 (td, 1H), 2.79 (t, 1H), 1.58 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 451.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80.0 mg, 0.235 mmol), tetrahydro-2H-pyran-4-amine (71.0 mg, 0.702 mmol) and DIPEA (100.0 mg, 0.774 mmol) in NMP (3 mL) was heated at 120° C. with stirring for 48 hrs. The mixture was poured into brine (4 mL) and extracted with DCM (3 mL) for 4 times. The combined organic layers were washed sequentially with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-N-tetrahydropyran-4-yl-pyridin-4-amine (22.1 mg) as a light yellow solid and 4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-N-tetrahydropyran-4-yl-pyridin-2-amine (6.0 mg) as a light yellow solid.
2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-N-tetrahydropyran-4-yl-pyridin-4-amine, 1H NMR (400 MHz CDCl3) δ ppm: 9.03 (d, 2H), 8.80 (s, 1H), 7.43 (t, 1H), 5.59-5.73 (m, 2H), 5.51 (s, 1H), 4.27-4.40 (m, 1H), 4.10-4.20 (m, 1H), 3.96-4.07 (m, 2H), 3.53 (s, 4H), 3.23 (br. s., 2H), 2.03 (d, 2H), 1.45-1.59 (m, 5H). MS obsd. (ESI+) [(M+H)+]: 422.
4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-N-tetrahydropyran-4-yl-pyridin-2-amine, 1H NMR (400 MHz CDCl3) δ ppm: 9.03 (d, 2H), 8.79 (s, 1H), 7.43 (s, 1H), 5.73-5.82 (m, 1H), 5.59-5.70 (m, 1H), 5.45-5.56 (m, 1H), 4.33-4.47 (m, 2H), 3.94-4.08 (m, 2H), 3.64-3.79 (m, 1H), 3.49-3.60 (m, 2H), 3.37-3.47 (m, 1H), 3.15-3.31 (m, 2H), 1.98-2.10 (m, 2H), 1.56 (d, 5H). MS obsd. (ESI+)[(M+H)+]: 422.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 340 mg, 1 mmol) and potassium carbonate (276 mg, 2 mmol), 1-tert-butyl 2-methyl piperazine-1,2-dicarboxylate (366 mg, 1.5 mmol) in DMSO (15 mL) were heated at 120° C. with stirring for 10 hrs. After being cooled to rt, the mixture was diluted with water (50 mL) and extracted with EA (80 mL). The organic layer was washed with water and brine, dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The residue was purified by flash chromatography (eluting with DCM/MeOH=10/1, v:v) to give 1-tert-butyl 2-methyl 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-1,2-dicarboxylate (282 mg) as a red oil.
To a solution of 1-tert-butyl 2-methyl 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-1,2-dicarboxylate (282 mg, 0.5 mol) in methanol (5 mL), THF (2 mL) and water (1 mL) was added LiOH (84 mg, 2 mol). The resulting mixture was stirred overnight at rt and then acidified to pH=6-7 with 2.0 M hydrochloric acid. The mixture was extracted with DCM (50 mL) twice and the combined organic layers were washed with water and brine, dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give crude 1-tert-butoxycarbonyl-4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-2-carboxylic acid (275 mg) which was directly used in the next step without purification.
To a solution of 1-tert-butoxycarbonyl-4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-2-carboxylic acid (275 mg, 0.5 mmol) in DCM (5 mL) was added TFA (1 mL) drop wise at 0° C. and the resulting mixture was stirred for 4 hrs at rt. The resulting reaction mixture was poured into ice water, and then adjusted pH to 8 with NH4OH. The resulting mixture was extracted with DCM (50 mL) twice and the combined organic layers were dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-2-carboxylic acid (8 mg) as a white powder. 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.93 (d, 2H), 8.79 (s, 1H), 7.55 (s, 1H), 6.06-6.16 (m, 1H), 5.78-5.87 (m, 1H), 5.60-5.72 (m, 1H), 4.39-4.52 (m, 1H), 4.11-4.23 (m, 1H), 3.79-3.92 (m, 1H), 3.58-3.71 (m, 1H), 3.37-3.48 (m, 1H), 3.27-3.36 (m, 1H), 2.95-3.18 (m, 5H), 1.49 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 451.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), isonipecotic acid (114 mg, 0.88 mmol) and K2CO3 (122 mg, 0.88 mmol) in NMP (2 mL) was heated at 170° C. in a microwave reactor for 1 hr. The resulting mixture was diluted with H2O (10 mL) and extracted with DCM (100 mL). The organic layer was washed with H2O (10 mL). The combined aqueous layer was concentrated in vacuo and the residue was purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperidine-4-carboxylic acid (30 mg) as a light yellow solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperidine-4-carboxylic acid (30 mg) as a light yellow solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperidine-4-carboxylic acid, 1H NMR (400 MHz, CDCl3) δ ppm: 9.05 (d, 2H), 8.82 (s, 1H), 7.44 (t, 1H), 5.78 (m, 2H), 5.63 (q, 1H), 4.44 (d, 1H), 4.20 (d, 2H), 3.36-3.51 (m, 1H), 3.16-3.32 (m, 2H), 3.01 (t, 2H), 2.54-2.70 (m, 1H), 2.05 (d, 2H), 1.77-1.85 (m, 2H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 450.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperidine-4-carboxylic acid, 1H NMR (400 MHz, CDCl3) δ ppm: 9.04 (d, 2H), 8.82 (s, 1H), 7.44 (t, 1H), 5.85 (s, 1H), 5.63-5.78 (m, 2H), 4.33-4.48 (m, 1H), 3.80 (d, 2H), 3.40-3.56 (m, 1H), 3.18-3.36 (m, 2H), 2.96-3.11 (m, 2H), 2.56-2.70 (m, 1H), 2.06 (d, 2H), 1.80-1.91 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 450.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), azetidine-3-carboxylic acid (89 mg, 0.88 mmol) and K2CO3 (122 mg, 0.88 mmol) in NMP (1 mL) was heated at 170° C. in a microwave reactor for 1 hr. The reaction mixture was then diluted with H2O (10 mL) and extracted with DCM (100 mL). The organic layer was washed with H2O (10 mL). The combined aqueous layer was concentrated in vacuo and the residue was purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidine-3-carboxylic acid (12.5 mg) as a light yellow solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidine-3-carboxylic acid (33.7 mg) as a light yellow solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidine-3-carboxylic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.97 (dd, 1H), 5.75 (q, 1H), 5.45 (m, 1H), 4.48-4.60 (m, 1H), 4.04-4.18 (m, 4H), 3.40-3.53 (m, 2H), 3.05-3.23 (m, 2H), 1.52-1.61 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidine-3-carboxylic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.69 (q, 1H), 5.63 (s, 1H), 5.38 (d, 1H), 4.41-4.52 (m, 1H), 4.03-4.21 (m, 4H), 3.40-3.64 (m, 2H), 3.05-3.23 (m, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), piperidine-4-carboxamide (90 mg, 0.71 mmol) and K2CO3 (98 mg, 0.71 mmol) in NMP (1 mL) was heated at 170° C. in a microwave reactor for 1 hr and then partitioned between DCM (80 mL) and H2O (20 mL). The organic layer was separated, then washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperidine-4-carboxamide (23 mg) as a light yellow solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperidine-4-carboxamide (42 mg) as a light yellow solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperidine-4-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.89 (s, 1H), 7.64 (t, 1H), 5.96 (d, 1H), 5.85 (d, 1H), 5.74 (m, 1H), 4.46-4.56 (m, 1H), 4.36 (d, 2H), 3.41-3.55 (m, 1H), 3.07-3.19 (m, 2H), 2.79-2.94 (m, 2H), 2.41-2.54 (m, 1H), 1.86 (d, 2H), 1.63-1.77 (m, 2H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 449.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperidine-4-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 1H), 8.85 (s, 1H), 7.63 (t, 1H), 6.07 (s, 1H), 5.81 (s, 1H), 5.70 (q, 1H), 4.48 (dd, 1H), 3.99 (d, 2H), 3.40-3.56 (m, 1H), 3.05-3.24 (m, 2H), 2.93 (t, 2H), 2.49 (m, 1H), 1.87 (d, 2H), 1.65-1.81 (m, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 449.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 1-(methylsulfonyl) piperazine (145 mg, 0.88 mmol) and K2CO3 (122 mg, 0.88 mmol) in NMP (2 mL) was heated at 180° C. in a microwave reactor for 2 hrs. The mixture was diluted with DCM (10 mL) and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[4-fluoro-6-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (28 mg) as a light yellow solid and 6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (35 mg) as a light yellow solid.
6-[4-fluoro-6-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.65 (t, 1H), 6.05 (d, 1H), 5.91 (d, 1H), 5.72-5.80 (m, 1H), 4.52 (d, 1H), 3.64-3.71 (m, 4H), 3.44-3.60 (m, 1H), 3.27-3.30 (m, 4H), 3.11-3.20 (m, 2H), 2.86 (s, 3H), 1.59 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 485.
6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.65 (t, 1H), 6.13 (s, 1H), 5.88 (s, 1H), 5.74 (q, 1H), 4.45-4.56 (m, 1H), 3.45-3.56 (m, 5H), 3.34 (m, 4H), 3.08-3.19 (m, 2H), 2.87 (s, 3H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 485.
To a solution of O1-benzyl O3-ethyl 2-methyl-4-oxo-piperidine-1,3-dicarboxylate (75.0 g, 235 mmol) in 2,2,2-trifluoroethanol (750 mL) was added 2-amidinopyridine hydrochloride (38.9 g, 247 mmol) and K2CO3 (97.4 g, 705 mmol). The mixture was heated at 80° C. with stirring for 12 hrs. After being cooled to rt, the mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified column chromatography (eluting with DCM/MeOH=20/1, v:v) to give benzyl 4-hydroxy-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (60.0 g) as a yellow solid.
To a solution of benzyl 4-hydroxy-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (60.0 g, 159 mmol) in MeCN (500 mL) was added POCl3 (122.2 g, 797 mmol) drop-wise. The mixture was heated at 60° C. with stirring for 3 hrs, and then poured into water and stirred for additional 10 mins. The resulting mixture was concentrated in vacuo to remove the organic solvent. The resulting aqueous mixture was adjusted to pH 7-8 with saturated aqueous NaHCO3, and then extracted with EA (300 mL) twice. The combined organic layer was washed with brine (500 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give benzyl 4-chloro-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (60.0 g) as a yellow oil.
To a solution of benzyl 4-chloro-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine-6-carboxylate (30.0 g, 76.2 mmol) in a mixture of EtOH/NH4OH/H2O (600 mL/150 mL/150 mL) was added Pd/C (6.0 g, 10% wt). The resulting mixture was stirred at rt under H2 (30 psi) for 12 hrs. The reaction was conducted at the same scale in parallel for four times. The reactions mixtures were filtered, and the combined filtrate were concentrated in vacuo.
The residue was dissolved in MeOH (200 mL) and DCM (2.0 L), and then treated with K2CO3 (210.6 g, 152.4 mmol). The resulting mixture was stirred at rt for 1 hr, and then filtered. The filtrate was concentrated in vacuo to give 5-methyl-2-(2-pyridyl)-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine (60.0 g, crude) as a yellow oil, which was used in the next step without any further purification.
A mixture of 5-methyl-2-(2-pyridyl)-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine (1.5 g, 6.6 mmol), 2,4,6-trifluoropyridine (1.1 g, 8.0 mmol) and DIPEA (2.6 g, 19.8 mmol) in NMP (15 mL) was heated at 150° C. in a microwave reactor with stirring for 1 hr. The resulting mixture was partitioned between H2O (40 mL) and DCM (40 mL). The aqueous layer was extracted with DCM (100 mL). The combined organic layer was washed with brine (200 mL) and concentrated in vacuo. The residue was purified by prep-HPLC to give 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (400 mg) as a yellow solid.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (100 mg, 0.295 mmol), 3-pyrrolidinol (77 mg, 0.884 mmol) and K2CO3 (122 mg, 0.884 mmol) in DMA (2 mL) was heated at 110° C. in a microwave reactor for 1.5 hrs.
The mixture was filtered and the filtrate was partitioned between EA (200 mL) and brine (200 mL). The organic layer was washed with brine (200 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]pyrrolidin-3-ol (12 mg) as a yellow solid and 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]pyrrolidin-3-ol (5 mg) as a yellow solid.
1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]pyrrolidin-3-ol, 1H NMR (400 MHz, CDCl3) δ ppm: 8.85 (d, 1H), 8.71 (s, 1H), 8.50 (d, 1H), 7.83-7.90 (m, 1H), 7.36-7.43 (m, 1H), 5.75 (d, 1H), 5.66 (br d, 1H), 5.49 (d, 1H), 4.62 (br s, 1H), 4.44 (br d, 1H), 3.52-3.70 (m, 4H), 3.38-3.47 (m, 1H), 3.10-3.23 (m, 2H), 2.03-2.22 (m, 2H), 1.57 (br s, 3H). MS obsd (ESI) [(M+H)+]: 407.
1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]pyrrolidin-3-ol, 1H NMR (400 MHz, CDCl3) δ ppm: 8.85 (d, 1H), 8.71 (s, 1H), 8.50 (d, 1H), 7.87 (t, 1H), 7.38-7.43 (m, 1H), 5.67 (br d, 1H), 5.55 (s, 1H), 5.48 (s, 1H), 4.65 (br s, 1H), 4.39 (br d, 1H), 3.53-3.60 (m, 2H), 3.41-3.51 (m, 2H), 3.33 (br d, 1H), 3.14-3.25 (m, 2H), 2.07-2.21 (m, 2H), 1.54-1.56 (d, 3H). MS obsd (ESI) [(M+H)+]: 407.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (product of step 4 in Example 59, 100 mg, 0.295 mmol), pyrrolidine-3-carboxamide hydrochloride (133 mg, 0.884 mmol) and K2CO3 (244 mg, 1.768 mmol) in NMP (3 mL) was heated at 130° C. with stirring for 16 hrs. After being cooled to rt, the mixture was filtered and the filtrate was partitioned between EA (200 mL) and brine (200 mL). The organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]pyrrolidine-3-carboxamide (9 mg) as a white solid and 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]pyrrolidine-3-carboxamide (20 mg) as a white solid.
1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]pyrrolidine-3-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 8.85 (d, 1H), 8.71 (s, 1H), 8.50 (d, 1H), 7.84-7.90 (m, 1H), 7.41 (dd, 1H), 5.76 (d, 1H), 5.56-5.68 (m, 2H), 5.49 (br d, 2H), 4.45 (br d, 1H), 3.74-3.82 (m, 1H), 3.60-3.73 (m, 2H), 3.38-3.51 (m, 2H), 3.03-3.22 (m, 3H), 2.26-2.34 (m, 2H), 1.55 (d, 3H). MS obsd (ESI) [(M+H)+]: 434.
1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]pyrrolidine-3-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 8.85 (d, 1H), 8.71 (s, 1H), 8.50 (d, 1H), 7.87 (td, 1H), 7.37-7.44 (m, 1H), 5.65 (br d, 1H), 5.46-5.59 (m, 4H), 4.40 (br d, 1H), 3.59-3.65 (m, 2H), 3.52-3.58 (m, 1H), 3.38-3.49 (m, 2H), 3.09-3.29 (m, 3H), 2.29-2.37 (m, 2H), 1.53-1.56 (d, 3H). MS obsd (ESI) [(M+H)+]: 434.
A mixture of 1-benzyloxycarbonylazetidine-3-carboxylic acid (500 mg, 2.13 mmol) and CDI (414 mg, 2.55 mmol) in DMF (5 mL) was heated at 65° C. with stirring for 1 hr. The resulting mixture was cooled to 0° C., then to the cooled mixture was added a mixture of NaH (510 mg, 12.7 mmol) and methanesulfonamide (607 mg, 6.38 mmol) in DMF (5 mL). The resulting mixture was warmed to rt and stirred for 1 hr, then neutralized with formic acid and extracted with EA (200 mL). The organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo to afford benzyl 3-(methylsulfonylcarbamoyl)azetidine-1-carboxylate (900 mg, crude) as a colorless oil, which was used in the next step without any further purification.
To a solution of benzyl 3-(methylsulfonylcarbamoyl)azetidine-1-carboxylate (900 mg, crude) in MeOH (15 mL) was added Pd/C (100 mg, 10% wt). The resulting mixture was stirred under H2 atmosphere (H2 balloon) at rt for 12 hrs, and then filtered. The filter cake was washed sequentially with MeOH (20 mL) and H2O (30 mL). The filtrate was concentrated in vacuo to give N-methylsulfonylazetidine-3-carboxamide (200 mg) as a white solid, which was used in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), K2CO3 (122 mg, 0.88 mmol) and N-methylsulfonylazetidine-3-carboxamide (63 mg, 0.35 mmol) in NMP (1 mL) was heated at 150° C. in a microwave reactor for 1 hr. The resulting mixture was diluted with H2O (20 mL) and extracted with EA (80 mL). The organic layer was washed with H2O (20 mL) and the combined aqueous layer was concentrated in vacuo. The residue was purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-N-methylsulfonyl-azetidine-3-carboxamide (17.2 mg) as a purple solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methylsulfonyl-azetidine-3-carboxamide (56.7 mg) as a light yellow solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-N-methylsulfonyl-azetidine-3-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 1H), 8.87 (s, 1H), 7.64 (t, 1H), 5.99 (m, 1H), 5.75 (q, 1H), 5.48 (m, 1H), 4.50-4.63 (m, 1H), 4.05-4.17 (m, 4H), 3.39-3.58 (m, 2H), 3.20 (s, 3H), 3.06-3.17 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 499.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methylsulfonyl-azetidine-3-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.86 (s, 1H), 7.64 (t, 1H), 5.69 (q, 1H), 5.63 (s, 1H), 5.38 (d, 1H), 4.46 (dd, 1H), 4.04-4.20 (m, 4H), 3.54-3.66 (m, 1H), 3.39-3.51 (m, 1H), 3.27 (s, 3H), 3.05-3.22 (m, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 499.
To a solution of 1-benzyloxycarbonylpiperidine-4-carboxylic acid (5.0 g, 19.0 mmol) in DMF (30 mL) was added CDI (9.24 g, 57.0 mmol). The resulting mixture was heated at 65° C. with stirring for 1 hr and then cooled to 0° C. To the cooled mixture was added a mixture of NaH (3.8 g, 95.0 mmol) and methanesulfonamide (10.8 g, 114.0 mmol) in DMF (30 mL) slowly. The resulting mixture was stirred at rt for 12 hrs, and then partitioned between EA (500 mL) and H2O (100 mL). The separated organic layer was washed with brine (100 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give benzyl 4-(methylsulfonylcarbamoyl)piperidine-1-carboxylate (6.0 g) as a yellow solid.
To a solution of benzyl 4-(methylsulfonylcarbamoyl)piperidine-1-carboxylate (1.0 g, 2.94 mmol) in MeOH (15 mL) was added Pd/C (100 mg, 10% wt). The mixture was stirred at rt under H2 (H2 balloon) for 12 hrs, and then filtered. The filter cake was washed sequentially with MeOH (20 mL) and H2O (30 mL). The aqueous solution was concentrated in vacuo to give N-methylsulfonylpiperidine-4-carboxamide (400 mg) as a white solid, which was used in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.059 mmol), K2CO3 (122 mg, 0.88 mmol) and N-methylsulfonylpiperidine-4-carboxamide (73 mg, 0.35 mmol) in NMP (0.5 mL) was heated at 150° C. in a microwave reactor for 1 hr. The resulting mixture was partitioned between EA (80 mL) and H2O (20 mL). The separated organic layer was washed with H2O (20 mL). The combined aqueous layers were concentrated in vacuo and the residue was purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-N-methylsulfonyl-piperidine-4-carboxamide (12 mg) as a light yellow solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methylsulfonyl-piperidine-4-carboxamide (42 mg) as a light yellow solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-N-methylsulfonyl-piperidine-4-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 1H), 8.88 (s, 1H), 7.64 (t, 1H), 5.97 (d, 1H), 5.85 (d, 1H), 5.74 (d, 1H), 4.50 (d, 1H), 4.33 (d, 2H), 3.42-3.54 (m, 1H), 3.21 (s, 3H), 3.07-3.18 (m, 1H), 2.81-2.96 (m, 1H), 2.52 (t, 1H), 1.83-1.94 (m, 2H), 1.69 (m, 2H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 527.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methylsulfonyl-piperidine-4-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.89 (s, 1H), 7.66 (t, 1H), 6.10 (s, 1H), 5.84 (s, 1H), 5.74 (q, 1H), 4.44-4.57 (m, 1H), 3.99 (d, 2H), 3.43-3.58 (m, 1H), 3.24 (s, 3H), 3.09-3.21 (m, 2H), 2.92-3.04 (m, 2H), 2.55 (m, 1H), 1.93 (d, 2H), 1.69-1.85 (m, 2H), 1.59 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 527.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 100 mg, 0.3 mmol), methyl pyrrolidine-3-carboxylate (116 mg, 0.9 mmol) and K2CO3 (124 mg, 0.9 mmol) in NMP (1 mL) was heated at 120° C. with stirring for 12 hrs. After being cooled to rt, the mixture was filtered and the filtrate was concentrated in vacuo. The residue was dissolved in MeOH (1 mL), and then stirred with a solution of NaOH (63 mg, 1.56 mmol) in H2O (1 mL) at 30° C. for 12 hrs. After being acidified to pH=7 with 1 M aqueous HCl, the resulting mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]pyrrolidine-3-carboxylic acid (10 mg) as a white solid and 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]pyrrolidine-3-carboxylic acid (11 mg) as a white solid.
1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]pyrrolidine-3-carboxylic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.71-8.81 (m, 2H) 8.52 (br d, 1H) 8.00 (br t, 1H) 7.49-7.60 (m, 1H) 7.49-7.60 (m, 1H) 5.85-6.06 (m, 1H) 5.78 (br dd, 1H) 5.57 (br dd, 1H) 4.24-4.75 (m, 2H) 3.38-3.60 (m, 3H) 2.96-3.22 (m, 2H) 2.22-2.40 (m, 1H) 2.02-2.17 (m, 3H) 1.49-1.62 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]pyrrolidine-3-carboxylic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.79 (s, 1H) 8.73 (br d, 1H) 8.52 (d, 1H) 8.00 (t, 1H) 7.54 (dd, 1H) 5.72 (s, 1H) 5.54-5.66 (m, 1H) 5.50 (br s, 1H) 4.43 (br dd, 1H) 4.09-4.21 (m, 1H) 3.59 (br s, 1H) 3.35-3.52 (m, 2H) 3.01-3.17 (m, 2H) 2.26-2.43 (m, 1H) 2.08-2.22 (m, 2H) 2.01-2.07 (m, 1H) 1.55 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 136 mg, 0.4 mmol), 1-(piperazin-1-yl)ethanone (154 mg, 1.2 mmol) and K2CO3 (166 mg, 1.2 mmol) in DMA (1 mL) was heated at 110° C. with stirring for 20 hrs. After being cooled to rt, the reaction mixture was filtered and the filtrate was purified by prep-HPLC to afford 1-[4-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperazin-1-yl]ethanone (60 mg) as a yellow powder and 1-[4-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]piperazin-1-yl]ethanone (17 mg) as a yellow powder.
1-[4-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperazin-1-yl]ethanone, 1H NMR (400 MHz, CDCl3) δ ppm: 8.83 (dd, 1H), 8.70 (s, 1H), 8.49 (d, 1H), 7.85 (dt, 1H), 7.39 (ddd, 1H), 5.82 (s, 1H), 5.69 (d, 1H), 5.63 (q, 1H), 4.42-4.32 (m, 1H), 3.81-3.71 (m, 2H), 3.67-3.58 (m, 2H), 3.53-3.31 (m, 5H), 3.28-3.11 (m, 2H), 2.14 (s, 3H), 1.55 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 448.
1-[4-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]piperazin-1-yl]ethanone, 1H NMR (400 MHz, CDCl3) δ ppm: 8.85 (dd, 1H), 8.72 (s, 1H), 8.51 (d, 1H), 7.87 (dt, 1H), 7.41 (ddd, 1H), 5.86 (dd, 1H), 5.74 (dd, 1H), 5.57 (q, 1H), 4.47-4.37 (m, 1H), 3.76 (dd, 2H), 3.67-3.57 (m, 4H), 3.53-3.41 (m, 3H), 3.23-3.16 (m, 2H), 2.17 (s, 3H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 448.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 136 mg, 0.4 mmol), 1-(methylsulfonyl)piperazine (197 mg, 1.2 mmol) and K2CO3 (166 mg, 1.2 mmol) in DMA (1 mL) was heated at 110° C. with stirring for 20 hrs. After being cooled to rt, the reaction mixture was filtered and the filtrate was purified by prep-HPLC to afford 6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (65 mg) as a yellow powder and 6-[4-fluoro-6-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (13 mg) as a yellow powder.
6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.88-8.82 (m, 1H), 8.72 (s, 1H), 8.50 (d, 1H), 7.87 (dt, 1H), 7.41 (ddd, 1H), 5.85 (s, 1H), 5.73 (d, 1H), 5.65 (q, 1H), 4.42-4.33 (m, 1H), 3.54-3.44 (m, 5H), 3.40-3.34 (m, 4H), 3.24-3.17 (m, 2H), 2.84 (s, 3H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 484.
6-[4-fluoro-6-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.86 (dd, 1H), 8.73 (s, 1H), 8.52 (d, 1H), 7.88 (dt, 1H), 7.42 (ddd, 1H), 5.88 (dd, 1H), 5.76 (dd, 1H), 5.56 (q, 1H), 4.48-4.38 (m, 1H), 3.72-3.64 (m, 4H), 3.52-3.42 (m, 1H), 3.35 (t, 4H), 3.24-3.17 (m, 2H), 2.83 (s, 3H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 484.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 136 mg, 0.4 mmol), piperidine-4-carboxamide (154 mg, 1.2 mmol) and K2CO3 (166 mg, 1.2 mmol) in DMA (1 mL) was heated at 110° C. with stirring for 20 hrs. After being cooled to rt, the reaction mixture was filtered and the filtrate was purified by prep-HPLC to afford 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperidine-4-carboxamide (65 mg) as a yellow powder and 1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]piperidine-4-carboxamide (25 mg) as a yellow powder.
1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperidine-4-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 8.86-8.82 (m, 1H), 8.72 (s, 1H), 8.50 (d, 1H), 7.86 (dt, 1H), 7.40 (ddd, 1H), 5.87 (s, 1H), 5.81 (br s, 1H), 5.74 (s, 1H), 5.70-5.60 (m, 2H), 4.44-4.33 (m, 1H), 3.85 (dd, 1H), 3.68 (br d, 1H), 3.52-3.40 (m, 1H), 3.28-3.12 (m, 3H), 3.08-2.98 (m, 1H), 2.57-2.43 (m, 1H), 2.09-1.99 (m, 1H), 1.90-1.61 (m, 3H), 1.55 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 448.
1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]piperidine-4-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 8.89-8.83 (m, 1H), 8.72 (s, 1H), 8.51 (d, 1H), 7.87 (dt, 1H), 7.41 (ddd, 1H), 5.78 (ddd, 2H), 5.62-5.38 (m, 3H), 4.48-4.38 (m, 1H), 4.32 (br d, 2H), 3.49-3.41 (m, 1H), 3.28-3.11 (m, 2H), 2.98-2.87 (m, 2H), 2.43 (tt, 1H), 1.98 (br d, 2H), 1.78 (dq, 2H), 1.57 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 448.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 136 mg, 0.4 mmol), piperidine-3-carboxamide (154 mg, 1.2 mmol) and K2CO3 (166 mg, 1.2 mmol) in DMA (1 mL) was heated at 110° C. for 20 hrs. After being cooled to rt, the reaction mixture was filtered and the filtrate was purified by prep-HPLC to afford 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperidine-3-carboxamide (57 mg) as a yellow powder and 1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]piperidine-3-carboxamide (18 mg) as a yellow powder.
1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]piperidine-3-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 8.84 (d, 1H), 8.71 (s, 1H), 8.50 (d, 1H), 7.87 (dt, 1H), 7.44-7.37 (m, 1H), 5.84 (s, 1H), 5.72 (s, 1H), 5.68-5.56 (m, 3H), 4.44-4.30 (m, 1H), 3.88 (br d, 2H), 3.53-3.41 (m, 1H), 3.29-3.11 (m, 2H), 3.02-2.89 (m, 2H), 2.42 (tt, 1H), 1.98 (br d, 2H), 1.89-1.73 (m, 2H), 1.55 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 448.
1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]piperidine-3-carboxamide, 1H NMR (400 MHz, CDCl3) δ ppm: 8.90-8.81 (m, 1H), 8.72 (s, 1H), 8.51 (dd, 1H), 7.88 (dt, 1H), 7.41 (ddd, 1H), 6.33-6.14 (m, 1H), 5.87-5.74 (m, 2H), 5.64-5.39 (m, 2H), 4.47-4.35 (m, 1H), 3.98-3.86 (m, 1H), 3.82-3.61 (m, 2H), 3.54-3.41 (m, 1H), 3.33-3.12 (m, 3H), 2.55 (br dd, 1H), 2.10-1.88 (m, 2H), 1.82-1.63 (m, 2H), 1.57 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 448.
To a solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 200 mg, 588 μmol) in NMP (4 mL) was added benzenesulfonamide (102 mg, 646 μmol) and potassium carbonate (162 mg, 1.18 mmol). The reaction mixture was heated at 150° C. in a microwave reactor for 4 hrs. The reaction mixture was filtered and the filtrate was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]benzenesulfonamide (102 mg) as a light yellow solid and N-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]benzenesulfonamide (10 mg) as a light yellow solid.
N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]benzenesulfonamide, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.78 (s, 1H), 8.00-7.90 (m, 2H), 7.69-7.60 (m, 4H), 6.37-6.30 (m, 1H), 6.01-5.92 (m, 1H), 5.37-5.24 (m, 1H), 4.35-4.21 (m, 1H), 3.32-3.23 (m, 1H), 2.92-2.76 (m, 2H), 1.35 (d, 3H). [(M+H)+]: 478.
N-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]benzenesulfonamide, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.96 (s, 1H), 7.95-7.90 (m, 2H), 7.71-7.56 (m, 4H), 6.37 (s, 1H), 6.00 (s, 1H), 5.50-5.39 (m, 1H), 4.27-4.17 (m, 1H), 3.49-3.37 (m, 1H), 3.02-2.95 (m, 2H), 1.45 (d, 3H). [(M+H)+]: 478.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 130 mg, 383 μmol), 2,2-difluoroethanamine (93.2 mg, 1.15 mmol) and K2CO3 (159 mg, 1.15 mmol) in NMP (5 mL) was heated at 100° C. with stirring overnight. The resulting mixture was filtered and the filtrate was purified by prep-HPLC to give N-(2,2-difluoroethyl)-2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]pyridin-2-amine (10 mg) as a light yellow solid and N-(2,2-difluoroethyl)-4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]pyridin-2-amine (10 mg) as a light yellow solid.
N-(2,2-difluoroethyl)-2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]pyridin-2-amine, 1H NMR (400 MHz, CDCl3) δ ppm: 1.55 (d, 3H), 3.09-3.27 (m, 2H), 3.40-3.50 (m, 1H), 3.52-3.65 (m, 2H), 4.29-4.38 (m, 1H), 4.46 (br s, 1H), 5.55 (d, 1H), 5.61 (q, 1H), 5.70 (s, 1H), 5.76-6.08 (m, 1H), 7.42 (dd, 1H), 7.88 (td, 1H), 8.49 (d, 1H), 8.70 (s, 1H), 8.80-8.87 (m, 1H). MS obsd. (ESI+) [(M+H)+]: 401.
N-(2,2-difluoroethyl)-4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]pyridin-2-amine, 1H NMR (400 MHz, CDCl3) δ ppm: 1.55 (d, 3H), 3.11-3.25 (m, 2H), 3.37-3.49 (m, 1H), 3.69-3.83 (m, 2H), 4.32-4.44 (m, 1H), 4.51-4.64 (m, 1H), 5.50-5.62 (m, 2H), 5.76-6.13 (m, 2H), 7.36-7.45 (m, 1H), 7.82-7.92 (m, 1H), 8.46-8.54 (m, 1H), 8.70 (s, 1H), 8.80-8.88 (m, 1H). MS obsd. (ESI+) [(M+H)+]: 401.
To a solution of 1-benzyloxycarbonylazetidine-3-carboxylic acid (1.5 g, 6.38 mmol) in DMF (20 mL) was added NH4Cl (3.41 g, 63.8 mmol), DIPEA (4.12 g, 31.9 mmol) and HATU (3.64 g, 9.56 mmol). The mixture was stirred at rt for 12 hrs and diluted with H2O (20 mL). The resulting mixture was extracted with EA (80 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was suspended in MeOH (30 mL) and filtered. The filtrate was concentrated in vacuo to afford benzyl 3-carbamoylazetidine-1-carboxylate (700 mg, crude) as a yellow solid, which was used in the next step without any further purification.
To a solution of benzyl 3-carbamoylazetidine-1-carboxylate (500 mg, 2.13 mmol) in MeOH (10 mL) was added Pd/C (100 mg, 10% wt). The mixture was stirred at rt under H2 atmosphere (H2 balloon) for 12 hrs, and then filtered. The filtrate was concentrated in vacuo to afford azetidine-3-carboxamide (150 mg, crude) as a yellow oil, which was used in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 80 mg, 0.23 mmol), K2CO3 (98 mg, 0.71 mmol) and azetidine-3-carboxamide (35 mg, 0.35 mmol) in NMP (1 mL) was heated at 150° C. in a microwave reactor for 1 hr. The resulting mixture was partitioned between EA (10 mL) and H2O (5 mL). The separated organic layer was concentrated in vacuo. The residue was purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidine-3-carboxamide (15 mg) as an orange solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidine-3-carboxamide (43 mg) as an orange solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidine-3-carboxamide, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.88 (s, 1H), 7.64 (t, 1H), 7.47 (br. s., 1H), 6.95-7.08 (br. s., 1H), 6.07 (d, 1H), 5.61-5.75 (m, 1H), 5.51 (dl H), 4.52 (d, 1H), 4.02 (t, 2H), 3.88-3.96 (m, 2H), 3.31-3.41 (m, 2H), 2.95-3.11 (m, 2H), 1.49 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 421.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidine-3-carboxamide, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 1H), 8.91 (s, 1H), 7.64 (t, 1H), 7.51 (br. s., 1H), 7.06 (br. s., 1H), 5.57-5.73 (m, 2H), 5.40 (s, 1H), 4.40 (dd, 1H), 4.03 (m, 2H), 3.87-3.97 (m, 2H), 3.33-3.46 (m, 2H), 2.92-3.12 (m, 2H), 1.47 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 421.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 170 mg, 0.5 mmol), K2CO3 (207 mg, 1.5 mmol) and 3-methoxypyrrolidine hydrochloride (137 mg, 1.0 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs. The resulting mixture was poured into water (20 mL) and extracted with DCM (50 mL) twice. The organic layers were combined and washed with brine, then dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 6-[6-fluoro-4-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (23 mg) as yellow powder and 6-[4-fluoro-6-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (10 mg) as yellow powder.
6-[6-fluoro-4-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.81-8.91 (m, 1H), 8.67-8.75 (m, 1H), 8.43-8.54 (m, 1H), 7.80-7.96 (m, 1H), 7.38-7.47 (m, 1H), 5.61-5.73 (m, 1H), 5.51-5.57 (m, 1H), 5.43-5.50 (m, 1H), 4.35-4.46 (m, 1H), 4.08-4.16 (m, 1H), 3.41-3.53 (m, 5H), 3.39 (d, 3H), 3.10-3.30 (m, 2H), 2.17-2.26 (m, 1H), 2.02-2.14 (m, 1H), 1.47-1.59 (m, 3H). MS obsd. (ESI+) [(M+H)+]: 421.
6-[4-fluoro-6-(3-methoxypyrrolidin-1-yl)-2-pyridyl]-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 8.71-8.82 (m, 1H), 8.62 (s, 1H), 8.37-8.46 (m, 1H), 7.73-7.84 (m, 1H), 7.28-7.36 (m, 1H), 5.62-5.70 (m, 1H), 5.53-5.61 (m, 1H), 5.36-5.44 (m, 1H), 4.31-4.41 (m, 1H), 3.97-4.05 (m, 1H), 3.51 (d, 2H), 3.40-3.47 (m, 2H), 3.33-3.39 (m, 1H), 3.31 (d, 3H), 3.00-3.18 (m, 2H), 1.96-2.12 (m, 2H), 1.47 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 421.
To a solution of 1-benzyloxycarbonylpyrrolidine-3-carboxylic acid (2.0 g, 8.02 mmol) in DCM (20 mL) was added a solution of N,N′-dicyclohexylcarbodiimide (2.48 g, 12.03 mmol) in DCM (5 mL), followed by addition of methanesulfonamide (763 mg, 8.02 mmol) and 4-dimethylaminopyridine (980 mg, 8.02 mmol). The mixture was stirred at rt for 16 hrs, and then filtered. The filtrate was concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=40/1, v:v) to give benzyl 3-(methylsulfonylcarbamoyl)pyrrolidine-1-carboxylate (2.2 g) as a white solid.
To a solution of benzyl 3-(methylsulfonylcarbamoyl)pyrrolidine-1-carboxylate (2.2 g, 6.74 mmol) in MeOH (20 mL) was added Pd/C (400 mg, 10% wt). The mixture was stirred under H2 atmosphere (H2 balloon) at rt for 16 hrs. The mixture was then filtered and the filtrate was concentrated in vacuo to give N-methylsulfonylpyrrolidine-3-carboxamide (1.3 g, crude) as a white solid.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 4 in Example 59, 100 mg, 0.295 mmol), K2CO3 (122 mg, 0.884 mmol) and N-methylsulfonylpyrrolidine-3-carboxamide (170 mg, 0.884 mmol) in NMP (3 mL) was heated at 150° C. in a microwave reactor for 1 hr. The resulting mixture was filtered and the filtrate was partitioned between EA (200 mL) and brine (100 mL). The separated organic layer was dried over with anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]-N-methylsulfonyl-pyrrolidine-3-carboxamide (3 mg) as a white solid and 1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]-N-methylsulfonyl-pyrrolidine-3-carboxamide (8 mg) as a white solid.
1-[4-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-2-pyridyl]-N-methylsulfonyl-pyrrolidine-3-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.80 (s, 1H), 8.72 (br d, 1H), 8.51 (d, 1H), 7.99 (td, 1H), 7.50-7.55 (m, 1H), 5.88 (d, 1H), 5.73 (q, 1H), 5.49 (d, 1H), 4.64 (s, 1H), 4.53 (br d, 1H), 3.70 (br s, 1H), 3.53-3.64 (m, 2H), 3.37-3.49 (m, 2H), 3.04-3.15 (m, 5H), 2.22 (q, 2H), 1.56 (d, 3H). MS obsd (ESI) [(M+H)+]: 512.
1-[2-fluoro-6-[5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl]-4-pyridyl]-N-methylsulfonyl-pyrrolidine-3-carboxamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.81 (s, 1H), 8.72 (d, 1H), 8.52 (d, 1H), 7.99 (td, 1H), 7.50-7.56 (m, 1H), 5.72 (s, 1H), 5.63 (br d, 1H), 5.51 (s, 1H), 4.64 (s, 1H), 4.40-4.51 (m, 1H), 3.33-3.59 (m, 5H), 3.04-3.22 (m, 5H), 2.25 (q, 2H), 1.55 (d, 3H). MS obsd (ESI) [(M+H)+]: 512.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 300 mg, 881 μmol) and tert-butyl piperazine-1-carboxylate (330 mg, 1.76 mmol) in DMSO (3 mL) and DIPEA (3 mL) was heated at 130° C. with stirring for 20 hrs. The reaction mixture was cooled to rt and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give tert-butyl 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-1-carboxylate (0.4 g) as a yellow solid.
To a solution of tert-butyl 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazine-1-carboxylate (0.4 g) in MeOH (5 mL) was added a solution of HCl in MeOH (10%) (5 mL). The reaction was stirred for 18 hrs at rt and concentrated in vacuo. The residue was dissolved in DCM (10 mL) and cooled to 0° C. To the solution was added triethylamine (0.55 mL) and 2-methoxyethanesulfonyl chloride (162.8 mg) successively. The resulting mixture was slowly warmed to rt and stirred for 2 hours at rt. The reaction mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[6-fluoro-4-[4-(2-methoxyethylsulfonyl)piperazin-1-yl]-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (31 mg) as light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.90 (s, 1H), 7.63 (t, 1H), 6.16 (s, 1H), 5.93 (s, 1H), 5.75-5.66 (m, 1H), 4.52-4.43 (m, 1H), 3.66 (t, 2H), 3.51-3.44 (m, 4H), 3.46-3.40 (m, 1H), 3.37 (t, 2H), 3.29-3.20 (m, 7H), 3.12-2.94 (m, 2H), 1.49 (d, 3H). MS obsd (ESI) [(M+H)+]: 529.
To a solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 300 mg, 881 μmol) in DMSO (3 mL) and DIPEA (3 mL) was added tert-butyl piperidin-4-ylcarbamate (353 mg, 1.76 mmol) and the resulting mixture was heated at 130° C. with stirring for 20 hrs. The resulting mixture was cooled to rt and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give a mixture of tert-butyl N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4-piperidyl]carbamate and tert-butyl N-[l-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-4-piperidyl]carbamate (0.42 g) as a yellow solid.
To a solution tert-butyl N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4-piperidyl]carbamate and tert-butyl N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-4-piperidyl]carbamate (0.42 g) in DCM (5 mL) was added TFA (5 mL). The reaction was stirred for 18 hrs at rt and concentrated in vacuo. The residue was dissolved in DCM (10 mL) and cooled to 0° C. To the cooled solution was added triethylamine (0.56 mL) and methanesulfonic anhydride (140.4 mg) successively. After being slowly warmed to rt and stirred for 2 hrs, the resulting reaction mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4-piperidyl]methanesulfonamide (64 mg) as a light yellow solid and N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-4-piperidyl]methanesulfonamide (14 mg) as a light yellow solid.
N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4-piperidyl]methanesulfonamide, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.90 (s, 1H), 7.64 (t, 1H), 7.11 (d, 1H), 6.10 (s, 1H), 5.88 (s, 1H), 5.75-5.66 (m, 1H), 4.54-4.42 (m, 1H), 3.89-3.83 (m, 2H), 3.48-3.36 (m, 2H), 3.09-2.97 (m, 3H), 2.95 (s, 3H), 1.91-1.83 (m, 2H), 1.48 (d, 3H), 1.46-1.37 (m, 2H). MS obsd (ESI) [(M+H)+]: 499.
N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-4-piperidyl]methanesulfonamide, 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.90 (s, 1H), 7.63 (t, 1H), 7.08 (d, 1H), 6.10-6.02 (m, 1H), 6.00-5.93 (m, 1H), 5.73-5.63 (m, 1H), 4.51-4.40 (m, 1H), 4.23-4.10 (m, 2H), 3.47-3.38 (m, 2H), 3.07-2.97 (m, 3H), 2.94 (s, 3H), 1.93-1.83 (m, 2H), 1.50 (d, 3H), 1.47-1.38 (m, 2H). MS obsd (ESI) [(M+H)+]: 499.
To a solution of tert-butyl piperazine-1-carboxylate (1.5 g, 8.05 mmol) and triethylamine (2.18 g, 3 mL, 21.5 mmol) in DCM (10 mL) at 0° C. was added ethanesulfonyl chloride (1.04 g, 8.05 mmol). After being warmed to rt and stirred for 2 hrs, the resulting reaction mixture was diluted with H2O (40 mL) and extracted with EA (50 mL) for three times. The combined organic layer was dried over anhydrous Na2SO4 and concentrated in vacuo to give crude tert-butyl 4-(ethylsulfonyl)piperazine-1-carboxylate (2.3 g) as a brown oil, which was used in the next step directly without any further purification.
To a solution of crude tert-butyl 4-(ethylsulfonyl)piperazine-1-carboxylate (2.3 g, 8.26 mmol) in DCM (10 mL) was added TFA (5 mL) and the mixture was stirred at rt for 2 hrs. The resulting mixture was then concentrated in vacuo to give crude 1-(ethylsulfonyl)-piperazine (1.5 g) as a brown oil, which was used in the next step directly without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-(2-pyridyl)-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 150 mg, 441 μmol) and 1-(ethylsulfonyl)piperazine (236 mg, 1.32 mmol) in DMSO (3 mL) and DIPEA (5 mL) was heated at 150° C. with stirring overnight. The resulting mixture was diluted with H2O (40 mL) and extracted with EA (50 mL) for three times. The combined organic layer was dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 6-[4-(4-ethylsulfonylpiperazin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (30 mg) as a light yellow solid and 6-[6-(4-ethylsulfonylpiperazin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (10 mg) as a light yellow solid.
6-[4-(4-ethylsulfonylpiperazin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 1.40 (t, 3H), 1.57 (d, 3H), 2.91-3.08 (m, 2H), 3.22-3.29 (m, 2H), 3.38-3.53 (m, 9H), 4.31-4.47 (m, 1H), 5.65-5.77 (m, 2H), 5.85 (s, 1H), 7.45 (t, 1H), 8.82 (s, 1H), 9.03 (d, 2H). MS obsd. (ESI+) [(M+H)+]: 499.
6-[6-(4-ethylsulfonylpiperazin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, CDCl3) δ ppm: 1.40 (t, 3H), 1.58 (d, 3H), 2.99 (q, 2H), 3.15-3.36 (m, 2H), 3.36-3.50 (m, 5H), 3.54-3.72 (m, 4H), 4.41 (dt, 1H), 5.60 (q, 1H), 5.75 (d, 1H), 5.86 (d, 1H), 7.44 (t, 1H), 8.82 (s, 1H), 9.03 (d, 2H). MS obsd. (ESI+) [(M+H)+]: 499.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), K2CO3 (122 mg, 0.88 mmol) and 4,4-dimethylpyrrolidine-3-carboxylic acid hydrochloride (158 mg, 0.88 mmol) in NMP (1 mL) was heated at 190° C. in a microwave reactor for 1.5 hrs. The resulting mixture was partitioned between DCM (10 mL) and H2O (5 mL). The separated aqueous layer was acidified with 1 M HCl and then purified by prep-HPLC to afford 1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-4,4-dimethyl-pyrrolidine-3-carboxylic acid (5.4 mg) as a yellow solid and 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4,4-dimethyl-pyrrolidine-3-carboxylic acid (17.6 mg) as a yellow solid.
1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-4,4-dimethyl-pyrrolidine-3-carboxylic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.89 (s, 1H), 7.64 (t, 1H), 5.88 (d, 1H), 5.80 (m, 1H), 5.53-5.53 (m, 1H), 4.60 (s, 1H), 4.55 (m, 1H), 3.62-3.83 (m, 2H), 3.40-3.53 (m, 2H), 3.23 (m, 1H), 3.09-3.15 (m, 1H), 2.81 (m, 1H), 1.54-1.65 (m, 3H), 1.30 (s, 3H), 1.10 (s, 3H). MS obsd. (ESI+) [(M+H)+]: 464.
1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4,4-dimethyl-pyrrolidine-3-carboxylic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.65 (t, 1H), 5.64-5.77 (m, 2H), 5.51 (s, 1H), 4.48 (m, 1H), 3.66-3.78 (m, 1H), 3.60 (br t, 1H), 3.43-3.54 (m, 1H), 3.46 (s, 1H), 3.18-3.26 (m, 2H), 3.16-3.16 (m, 1H), 3.04-3.18 (m, 1H), 2.86-2.99 (m, 1H), 1.58 (dd, 3H), 1.32 (d, 3H), 1.11 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 464.
To a solution of tert-butyl piperazine-1-carboxylate (2.0 g, 10.74 mmol) in THF (20 mL) was added Et3N (2.17 g, 21.48 mmol) and tert-butyl bromoacetate (2.09 g, 10.74 mmol). The resulting mixture was stirred at rt for 12 hrs, and then partitioned between EA (200 mL) and water (100 mL). The organic layer was separated and washed with brine (100 mL), then dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with PE/EA=5/1, v:v) to give tert-butyl 4-(2-tert-butoxy-2-oxo-ethyl)piperazine-1-carboxylate (2.5 g) as a white solid.
A mixture of tert-butyl 4-(2-tert-butoxy-2-oxo-ethyl)piperazine-1-carboxylate (1.0 g, 3.3 mmol) and a solution of HCl in MeOH (1.0 M, 15 mL) was stirred at rt for 12 hrs. The resulting mixture was concentrated in vacuo. The residue was dissolved in MeO—H (10 mL), then treated with base resin and filtered. The filtrate was concentrated in vacuo to give 2-piperazin-1-ylacetic acid (350 mg) as a white solid.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.294 mmol), 2-piperazin-1-ylacetic acid (51 mg, 0.353 mmol) and K2CO3 (122 mg, 0.881 mmol) in NMP (3 mL) was heated at 180° C. in a microwave reactor for 1 hr, and then partitioned between EA (100 mL) and water (20 mL). The separated organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 2-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]acetic acid (13 mg) as a yellow solid and 2-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]acetic acid (3 mg) as a yellow solid.
2-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]acetic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.64 (t, 1H), 6.08 (s, 1H), 5.82 (s, 1H), 5.68-5.76 (m, 1H), 4.51 (br d, 1H), 3.40-3.47 (m, 5H), 3.09-3.26 (m, 2H), 3.03 (s, 2H), 2.62-2.71 (m, 4H), 1.57 (d, 3H). MS obsd (ESI) [(M+H)+]: 465.
2-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]acetic acid, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.90 (s, 1H), 7.64 (t, 1H), 5.98 (d, 1H), 5.68-5.87 (m, 2H), 4.50 (br d, 1H), 3.37-3.58 (m, 5H), 3.09-3.20 (m, 2H), 3.04 (s, 2H), 2.59-2.70 (m, 4H), 1.54-1.60 (m, 3H). MS obsd (ESI) [(M+H)+]: 465.
2-Fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-N-(1-methylsulfonyl-4-piperidyl)pyridin-4-amine
To a solution of 5-methyl-2-(pyrimidin-2-yl)-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine hydrochloride (the product of step 6 in Example 1, 2.5 g, 9.48 mmol) in DMSO (30 mL) was added 2,6-difluoro-4-iodopyridine (2.74 g, 11.4 mmol) and sodium bicarbonate (3.98 g, 47.4 mmol). The resulting mixture was heated at 80° C. with stirring for 18 hrs. The resulting reaction mixture was diluted with H2O (20 mL) and extracted with DCM (50 mL) twice. The combined organic layer was dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (1.9 g) as a brown solid.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (130 mg, 290 μmol), tert-butyl 4-aminopiperidine-1-carboxylate (69.7 mg, 348 μmol) and cesium carbonate (189 mg, 580 μmol) in dioxane (5 mL) was added Xantphos (33.6 mg, 58 μmol) and Pd(OAc)2 (6.51 mg, 29 μmol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under Ar. The resulting reaction mixture was filtered and the filtrate was concentrated in vacuo to give crude tert-butyl 4-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]piperidine-1-carboxylate (0.2 g) which was directly used in the next step without further purification.
To a solution of crude tert-butyl 4-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]piperidine-1-carboxylate (200 mg) in DCM (5 mL) was added TFA (5 mL) and the reaction was stirred for 18 hrs at rt. The resulting reaction mixture was concentrated in vacuo and the residue was dissolved in DCM (10 mL). The resulting solution was cooled to 0° C. and to the cooled solution were added triethylamine (146 mg, 201 μL, 1.44 mmol) and methanesulfonic anhydride (50.2 mg, 288 μmol) successively. The resulting mixture was slowly warmed to rt and stirred for 2 hrs. The resulting reaction mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-N-(1-methylsulfonyl-4-piperidyl)pyridin-4-amine (11 mg) as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ ppm: 9.03 (d, 2H), 8.79 (s, 1H), 7.43 (t, 1H), 5.63 (s, 1H), 5.49 (s, 1H), 4.34-4.28 (m, 1H), 4.16-4.10 (m, 1H), 3.85-3.76 (m, 2H), 3.52-3.41 (m, 2H), 3.31-3.18 (m, 2H), 2.97-2.87 (m, 2H), 2.83 (s, 3H), 2.20-2.12 (m, 2H), 1.56 (d, 3H), 1.36-1.23 (m, 2H). MS obsd (ESI) [(M+H)+]: 499.
Separation of 6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (Example 58, 700 mg) by chiral preparative HPLC affords (−)-6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (330 mg) as a white solid and (+)-6-[6-fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (254 mg) as a white solid.
(−)-6-[6-Fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.91-8.98 (m, 2H), 8.75-8.80 (m, 1H), 7.52-7.58 (m, 1H), 6.01-6.07 (m, 1H), 5.76-5.81 (m, 1H), 5.61-5.69 (m, 1H), 4.37-4.46 (m, 1H), 3.36-3.48 (m, 5H), 3.28-3.21 (m, 4H), 2.98-3.15 (m, 2H), 2.79 (s, 3H), 1.49 (d, 3H). MS obsd (ESI) [(M+H)+]: 485. [a]D20=−102.2° (0.05 g/100 mL, methanol).
(+)-6-[6-Fluoro-4-(4-methylsulfonylpiperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.91-8.98 (m, 2H), 8.75-8.80 (m, 1H), 7.52-7.58 (m, 1H), 6.01-6.07 (m, 1H), 5.76-5.81 (m, 1H), 5.61-5.69 (m, 1H), 4.37-4.46 (m, 1H), 3.36-3.48 (m, 5H), 3.28-3.21 (m, 4H), 2.98-3.15 (m, 2H), 2.79 (s, 3H), 1.49 (d, 3H). MS obsd (ESI) [(M+H)+]: 485.
To a stirring solution of tert-butyl 3,3-dimethylpiperazine-1-carboxylate (300 mg, 1.4 mmol) in DCM (3 mL) was added MsCl (321 mg, 2.8 mmol) and K2CO3 (425 mg, 4.2 mmol). After being stirred at 20° C. for 4 hrs, the resulting mixture was diluted with DCM (80 mL), then washed with water (30 mL) and brine (30 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by column chromatography (eluting with PE/EA=5/1, v:v) to give tert-butyl 3,3-dimethyl-4-methylsulfonyl-piperazine-1-carboxylate (270 mg) as a colorless oil.
A mixture of tert-butyl 3,3-dimethyl-4-methylsulfonyl-piperazine-1-carboxylate (270 mg, 0.92 mmol) and a solution of HCl in MeOH (1.0 M, 3 mL) was stirred at rt for 4 hrs. The resulting reaction mixture was concentrated in vacuo to give 2,2-dimethyl-1-methylsulfonyl-piperazine (180 mg) as a white solid, which was used in the next step directly without any purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), K2CO3 (122 mg, 0.88 mmol) and 2,2-dimethyl-1-methylsulfonyl-piperazine (134 mg, 0.59 mmol) in NMP (1 mL) was heated at 190° C. with stirring in a microwave reactor for 1.5 hrs. The resulting reaction mixture was then diluted with DCM (10 mL) and washed with H2O (5 mL). The separated organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to afford 6-[6-(3,3-dimethyl-4-methylsulfonyl-piperazin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (9 mg) as a yellow solid and 6-[4-(3,3-dimethyl-4-methylsulfonyl-piperazin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (17 mg) as a white solid.
6-[6-(3,3-dimethyl-4-methylsulfonyl-piperazin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.91 (s, 1H), 7.65 (t, 1H), 5.99 (dd, 1H), 5.70-5.79 (m, 2H), 4.46-4.57 (m, 1H), 3.56-3.78 (m, 6H), 3.42-3.54 (m, 1H), 3.11-3.19 (m, 2H), 2.98 (s, 3H), 1.59 (d, 3H), 1.50 (d, 6H). MS obsd. (ESI+) [(M+H)+]: 513.
6-[4-(3,3-dimethyl-4-methylsulfonyl-piperazin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.04 (d, 2H), 8.88 (s, 1H), 7.66 (t, 1H), 5.98 (s, 1H), 5.78 (s, 1H), 5.71-5.77 (m, 1H), 4.52 (m, 1H), 3.65-3.75 (m, 2H), 3.52-3.59 (m, 2H), 3.44-3.52 (m, 2H), 3.06-3.25 (m, 2H), 2.99 (s, 3H), 1.58 (d, 3H), 1.52 (d, 6H). MS obsd. (ESI+) [(M+H)+]: 513.
To a stirred solution of 1-Boc-piperazine (1.0 g, 5.37 mmol) in THF (15 mL) was added 2-bromoacetamide (889 mg, 6.44 mmol) and Et3N (1.63 g, 16.1 mmol). After being stirred at rt for 12 hrs and diluted with EA (200 mL), the resulting mixture was washed with water (50 mL) and brine (50 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by flash column (eluting with PE/EA=1/1, v:v) to give tert-butyl 4-(2-amino-2-oxo-ethyl)piperazine-1-carboxylate (1.0 g) as a white solid.
A mixture of tert-butyl 4-(2-amino-2-oxo-ethyl)piperazine-1-carboxylate (400 mg, 1.64 mmol) and a solution of HCl in MeOH (5 mL) was stirred at rt for 4 hrs. The resulting mixture was concentrated in vacuo and the residue was dissolved in NMP (2 mL). To the resulting solution were added 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 150 mg, 0.44 mmol) and K2CO3 (183 mg, 1.32 mmol). The resulting mixture was heated at 170° C. with stirring in a microwave reactor for 2 hrs, then cooled to rt, diluted with DCM (10 mL) and washed with H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to afford 2-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]acetamide (33 mg) as a yellow solid and 2-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]acetamide (38 mg) as a yellow solid.
2-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]acetamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.89 (s, 1H), 7.65 (t, 1H), 6.02 (d, 1H), 5.86 (d, 1H), 5.75 (q, 1H), 4.51 (m, 1H), 3.54-3.69 (m, 4H), 3.48 (m, 1H), 3.03-3.26 (m, 4H), 2.62-2.79 (m, 4H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 464.
2-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]acetamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 6.09 (s, 1H), 5.83 (s, 1H), 5.72 (q, 1H), 4.44-4.56 (m, 1H), 3.47-3.55 (m, 1H), 3.41-3.47 (m, 4H), 3.12-3.22 (m, 2H), 3.10 (s, 2H), 2.62-2.75 (m, 4H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 464.
A mixture of 1-Boc-piperazine (500 mg, 2.68 mmol), acrylamide (210 mg, 2.95 mmol) and AcOH (16 mg, 0.27 mmol) was heated at 70° C. with stirring for 12 hrs. The resulting reaction mixture was diluted with DCM (100 mL), then washed with saturated aqueous solution of NaHCO3 (30 mL), water (30 mL) and brine (30 mL), dried over anhydrous Na2SO4 and concentrated in vacuo to give crude tert-butyl 4-(3-amino-3-oxo-propyl)piperazine-1-carboxylate (300 mg) as a white solid, which was used in the next step without any purification.
A mixture of tert-butyl 4-(3-amino-3-oxo-propyl)piperazine-1-carboxylate (300 mg, 1.17 mmol) and a solution of HCl in MeOH (5 mL, 1 M) was stirred at rt for 2 hrs. The resulting reaction mixture was concentrated in vacuo to give 3-piperazin-1-ylpropanamide (250 mg) as a white solid, which was used in the next step without any purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (150 mg, 0.44 mmol), K2CO3 (183 mg, 1.32 mmol) and 3-piperazin-1-ylpropanamide (one product of step 7 in Example 1, 171 mg, 0.88 mmol) in NMP (2 mL) was heated at 170° C. with stirring in a microwave reactor for 2 hrs. The resulting reaction mixture was diluted with DCM (10 mL) and washed with H2O (5 mL). The organic layer was concentrated in vacuo and the residue was purified by prep-HPLC to afford 3-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]propanamide (5 mg) as a yellow solid and 3-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]propanamide (26 mg) as a white solid.
3-[4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]piperazin-1-yl]propanamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.88 (s, 1H), 7.63 (t, 1H), 6.00 (d, 1H), 5.83 (d, 1H), 5.73 (q, 1H), 4.50 (m, 1H), 3.51-3.61 (m, 4H), 3.42-3.51 (m, 1H), 3.02-3.23 (m, 2H), 2.66-2.79 (m, 2H), 2.60 (m, 4H), 2.46 (t, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 478.
3-[4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-1-yl]propanamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.86 (s, 1H), 7.63 (t, 1H), 6.07 (s, 1H), 5.82 (s, 1H), 5.71 (q, 1H), 4.48 (m, 1H), 3.43-3.54 (m, 1H), 3.35-3.43 (m, 4H), 3.04-3.24 (m, 2H), 2.69-2.79 (m, 2H), 2.54-2.67 (m, 4H), 2.45 (t, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 478.
To a stirring solution of tert-butyl 3-methylpiperazine-1-carboxylate (400 mg, 2.0 mmol) in DCM (5 mL) was added Et3N (606 mg, 5.99 mmol) and MsCl (686 mg, 5.99 mmol). The resulting mixture was stirred at rt for 12 hrs and diluted with EA (100 mL). The resulting mixture was washed with water (30 mL) and brine (30 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by flash column (eluting with PE/EA=5/1, v:v) to give tert-butyl 3-methyl-4-methylsulfonyl-piperazine-1-carboxylate (400 mg) as a white solid.
A mixture of tert-butyl 3-methyl-4-methylsulfonyl-piperazine-1-carboxylate (400 mg, 1.44 mmol) and a solution of HCl in MeOH (1.0 M, 5 mL) was stirred at rt for 2 hrs. The resulting mixture was concentrated in vacuo and the residue was dissolved in MeOH (5 mL). The solution was stirred with basic resin (200 mg) at rt for 2 hrs and then filtered. The filtrate was concentrated in vacuo to give crude 2-methyl-1-methylsulfonyl-piperazine (250 mg) as a colorless oil which was used directly in the next step without any purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), K2CO3 (122 mg, 0.88 mmol) and 2-methyl-1-methylsulfonyl-piperazine (157 mg, 0.88 mmol) in NMP (1 mL) was heated at 180° C. with stirring for 2 hrs in a microwave reactor. After being cooled to rt, the resulting mixture was filtered and the filtrate was purified by prep-HPLC to afford 6-[4-fluoro-6-(3-methyl-4-methylsulfonyl-piperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (20.3 mg) as a yellow solid and 6-[6-fluoro-4-(3-methyl-4-methylsulfonyl-piperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (31 mg) as a yellow solid.
6-[4-fluoro-6-(3-methyl-4-methylsulfonyl-piperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.04 (d, 2H), 8.91 (s, 1H), 7.65 (t, 1H), 6.01 (m, 1H), 5.87 (d, 1H), 5.76 (t, 1H), 4.53 (br s, 1H), 4.25 (m, 1H), 4.22-4.31 (m, 1H), 4.17 (m, 1H), 4.05-4.34 (m, 1H), 3.64 (m, 1H), 3.43-3.57 (m, 1H), 3.34-3.41 (m, 1H), 3.13-3.14 (m, 1H), 3.12-3.23 (m, 2H), 3.06 (s, 1H), 2.96 (s, 3H), 1.55-1.66 (m, 3H), 1.22-1.38 (m, 3H). MS obsd. (ESI+)[(M+H)+]: 499.
6-[6-fluoro-4-(3-methyl-4-methylsulfonyl-piperazin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 6.07 (s, 1H), 5.83 (s, 1H), 5.73 (q, 1H), 4.45-4.55 (m, 1H), 4.06-4.20 (m, 1H), 3.87 (m, 1H), 3.71-3.83 (m, 1H), 3.64 (m, 1H), 3.35-3.54 (m, 2H), 3.22-3.29 (m, 1H), 3.04-3.20 (m, 3H), 2.95 (s, 3H), 1.57 (d, 3H), 1.33 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 499.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 204 mg, 0.6 mmol), 1-(methylsulfonyl)piperazine (123 mg, 0.75 mmol) and K2CO3 (165 mg, 1.2 mmol) in NMP (5 mL) was heated at 180° C. with stirring for 2 hrs in a microwave reactor. The resulting mixture was poured into water (20 mL) and extracted with DCM (50 mL) twice. The combined organic layer was washed with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 6-[2-fluoro-6-(4-methyl sulfonylpiperazin-1-yl)-4-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (18 mg) as a light yellow powder. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02-9.09 (m, 2H), 8.88-8.92 (m, 1H), 7.59-7.71 (m, 1H), 6.09-6.17 (m, 1H), 5.99-6.06 (m, 1H), 5.37-5.46 (m, 1H), 4.13-4.23 (m, 1H), 3.37-3.71 (m, 5H), 3.15-3.32 (m, 6H), 2.88 (s, 3H), 1.62 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 485.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), K2CO3 (207 mg, 1.5 mmol) and 3-methoxypyrrolidine hydrochloride (137 mg, 1.0 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs. The resulting mixture was poured into water (20 mL) and extracted with DCM (50 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 6-[2-fluoro-6-(3-methoxypyrrolidin-1-yl)-4-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (30 mg) as a white powder. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.90 (s, 1H), 7.62-7.70 (m, 1H), 5.89-5.97 (m, 1H), 5.67-5.75 (m, 1H), 5.33-5.43 (m, 1H), 4.12 (m, 2H), 3.42-3.64 (m, 5H), 3.39 (d, 3H), 3.14-3.28 (m, 2H), 2.04-2.21 (m, 2H), 1.61 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 422.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), azetidine (50.3 mg, 0.88 mmol) and K2CO3 (122 mg, 0.88 mmol) in NMP (1 mL) was heated at 130° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was diluted with DCM (5 mL) and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[6-(azetidin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (5.4 mg) as a red solid and 6-[4-(azetidin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (18.2 mg) as a yellow solid.
6-[6-(azetidin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.86 (s, 1H), 7.64 (t, 1H), 5.93 (dd, 1H), 5.74 (q, 1H), 5.40 (dd, 1H), 4.48-4.59 (m, 1H), 3.97 (t, 4H), 3.38-3.50 (m, 1H), 3.03-3.20 (m, 2H), 2.35 (m, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 378.
6-[4-(azetidin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.85 (s, 1H), 7.63 (t, 1H), 5.66 (q, 1H), 5.56 (s, 1H), 5.31 (d, 1H), 4.37-4.49 (m, 1H), 3.97 (t, 4H), 3.39-3.52 (m, 1H), 3.04-3.22 (m, 2H), 2.41 (m, 2H), 1.55 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 378.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 3-methoxyazetidine (76.8 mg, 0.88 mmol) and K2CO3 (121.8 mg, 0.88 mmol) in NMP (1 mL) was heated at 130° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was then diluted with DCM (5 mL) and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to give 6-[4-fluoro-6-(3-methoxyazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (8.4 mg) as a pink solid and 6-[6-fluoro-4-(3-methoxyazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (32.6 mg) as a yellow solid.
6-[4-fluoro-6-(3-methoxyazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.96 (dd, 1H), 5.74 (q, 1H), 5.46 (dd, 1H), 4.49-4.60 (m, 1H), 4.28-4.37 (m, 1H), 4.10-4.20 (m, 2H), 3.78 (m, 2H), 3.39-3.51 (m, 1H), 3.33 (s, 3H), 3.05-3.21 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 408.
6-[6-fluoro-4-(3-methoxyazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.85 (s, 1H), 7.63 (t, 1H), 5.68 (q, 1H), 5.61 (s, 1H), 5.36 (d, 1H), 4.44 (m, 1H) 4.40-4.50 (m, 1H), 4.30-4.40 (m, 1H), 4.15 (t, 2H), 3.77 (dd, 2H), 3.40-3.52 (m, 1H), 3.34 (s, 3H), 3.02-3.23 (m, 2H), 1.55 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 408.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added pyrrolidin-2-one (38 mg, 446 μmol), cesium carbonate (145 mg, 446 μmol), Xantphos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under Ar. The resulting reaction mixture was then filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]pyrrolidin-2-one (8 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.95 (s, 1H), 7.64 (t, 1H), 6.91 (s, 1H), 6.84 (s, 1H), 5.72-5.61 (m, 1H), 4.51-4.37 (m, 1H), 3.93-3.84 (m, 2H), 3.54-3.44 (m, 1H), 3.11-2.98 (m, 2H), 2.58-2.53 (m, 2H), 2.13-2.01 (m, 2H), 1.52 (d, 3H). MS obsd (ESI) [(M+H)+]: 406.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added 5,5-dimethyloxazolidin-2-one (51.4 mg, 446 μmol), cesium carbonate (145 mg, 446 μmol), Xantphos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). The reaction mixture was heated at 100° C. with stirring for 20 hrs under Ar. After being cooled to rt, the resulting mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 3-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-5,5-dimethyl-oxazolidin-2-one (8 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.95 (s, 1H), 7.64 (t, 1H), 6.72 (s, 1H), 6.70 (s, 1H), 5.71-5.66 (m, 1H), 4.51-4.40 (m, 1H), 3.93 (s, 2H), 3.56-3.44 (m, 1H), 3.12-2.97 (m, 2H), 1.53 (d, 3H), 1.49 (d, 6H). MS obsd (ESI) [(M+H)+]: 436.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added isothiazolidine 1,1-dioxide (54.1 mg, 446 μmol), cesium carbonate (145 mg, 446 μmol), Xantphos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). After being heated at 100° C. with stirring for 20 hrs under argon, the resulting reaction mixture was then filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 2-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-1,2-thiazolidine1,1-dioxide (8 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.95 (s, 1H), 7.64 (t, 1H), 6.32 (s, 1H), 6.15 (s, 1H), 5.69-5.64 (m, 1H), 4.47-4.36 (m, 1H), 3.89-3.79 (m, 2H), 3.68-3.59 (m, 2H), 3.54-3.43 (m, 1H), 3.14-2.98 (m, 2H), 2.42-2.37 (m, 2H), 1.52 (d, 3H). MS obsd (ESI) [(M+H)+]: 442.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 3-methylpiperazin-2-one (101 mg, 0.88 mmol) and DIPEA (0.15 mL, 0.88 mmol) in NMP (0.5 mL) was heated at 200° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-3-methyl-piperazin-2-one (4.5 mg) as a yellow solid and 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-methyl-piperazin-2-one (7.5 mg) as a light yellow solid.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-3-methyl-piperazin-2-one, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.91 (s, 1H), 7.66 (d, 1H), 6.03 (d, 1H), 5.85 (d, 1H), 5.68-5.79 (m, 1H), 4.44-4.67 (m, 2H), 4.07-4.25 (m, 1H), 3.37-3.61 (m, 4H), 3.16 (s, 2H), 1.60 (d, 3H), 1.47 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-methyl-piperazin-2-one, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.65 (t, 1H), 6.01 (d, 1H), 5.80 (s, 1H), 5.68-5.77 (m, 1H), 4.37-4.57 (m, 2H), 3.79-3.92 (m, 1H), 3.36-3.54 (m, 4H), 3.08-3.24 (m, 2H), 1.58 (dd, 3H), 1.47 (t, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
To a solution of 1-Boc-3-(amino)azetidine (1.0 g, 5.81 mmol) in DCM (10 mL) was added triethylamine (2.43 mL, 17.42 mmol) and methanesulfonyl chloride (0.67 mL, 8.71 mmol) at 0° C. After being warmed to rt and stirred at rt for 12 hrs, the resulting reaction mixture was diluted with DCM (80 mL) and washed with water (20 mL) twice and brine (20 mL). The organic layer was dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was then purified by column chromatography (eluting with PE/EA=5/1, v:v) to give tert-butyl 3-(methanesulfonamido)azetidine-1-carboxylate (1.2 g) as a yellow solid.
To a solution of tert-butyl 3-(methanesulfonamido)azetidine-1-carboxylate (300 mg, 1.2 mmol) in DCM (3 mL) was added TFA (1.0 mL) at 0° C. After being warmed to rt and stirred for 12 hrs, the resulting mixture was concentrated in vacuo to give crude N-(azetidin-3-yl)methanesulfonamide (160 mg) as a yellow oil which was used directly in the next step without any purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), N-(azetidin-3-yl)methanesulfonamide (132 mg, 0.88 mmol) and potassium carbonate (122 mg, 0.88 mmol) in NMP (1 mL) was heated at 140° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was then diluted with DCM (5 mL), filtered and concentrated in vacuo. The residue was purified by prep-HPLC to give N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidin-3-yl]methanesulfonamide (25 mg) as a yellow solid and N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-yl]methanesulfonamide (27 mg) as a yellow solid.
N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidin-3-yl]methanesulfonamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 6.00 (dd, 1H), 5.75 (q, 1H), 5.48 (dd, 1H), 4.50-4.59 (m, 1H), 4.35-4.43 (m, 1H), 4.30 (m, 2H), 3.84 (m, 2H), 3.40-3.51 (m, 1H), 3.08-3.21 (m, 2H), 2.97 (s, 3H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 471.
N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-yl]methanesulfonamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.69 (q, 1H), 5.64 (s, 1H), 5.39 (d, 1H), 4.40-4.53 (m, 2H), 4.32 (m, 2H), 3.83 (m, 2H), 3.42-3.55 (m, 1H), 3.09-3.19 (m, 2H), 2.97 (s, 3H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 471.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (100 mg, 0.29 mmol), 1-Boc-3-(amino)azetidine (one product of step 7 in Example 1, 152 mg, 0.88 mmol) and potassium carbonate (122 mg, 0.88 mmol) in NMP (0.5 mL) was heated at 170° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was then diluted with MeOH (3 mL), filtered and purified by prep-HPLC to afford tert-butyl 3-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]azetidine-1-carboxylate (25 mg) and tert-butyl 3-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]azetidine-1-carboxylate (25 mg).
To a stirring solution of tert-butyl 3-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]azetidine-1-carboxylate (25 mg, 0.05 mmol) in DCM (0.5 mL) was added trifluoroacetic acid (0.25 mL). The resulting mixture was stirred at rt for 12 hrs and then concentrated in vacuo. The residue was purified by prep-HPLC to give N-(azetidin-3-yl)-4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-2-amine (5 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.04 (d, 2H), 8.87 (s, 1H), 7.66 (t, 1H), 5.99 (m, 1H), 5.67-5.74 (m, 1H), 5.65 (m, 1H), 4.59 (br s, 1H), 4.47-4.56 (m, 1H), 4.30-4.45 (m, 2H), 4.03 (m, 2H), 3.42-3.54 (m, 1H), 3.10-3.18 (m, 2H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 393.
To a stirred solution of tert-butyl 3-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]azetidine-1-carboxylate (25 mg, 0.05 mmol) in DCM (0.5 mL) was added trifluoroacetic acid (0.25 mL). The resulting mixture was stirred at 20° C. for 12 hrs and then concentrated in vacuo. The residue was purified by prep-HPLC to give N-(azetidin-3-yl)-2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (14.4 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.65 (t, 1H), 5.80 (s, 1H), 5.69 (q, 1H), 5.54 (d, 1H), 4.58-4.67 (m, 1H), 4.37-4.48 (m, 3H), 3.96-4.05 (m, 2H), 3.45-3.55 (m, 1H), 3.11-3.18 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 393.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 3-fluoroazetidine (88.24 mg, 1.18 mmol) and potassium carbonate (162.4 mg, 1.18 mmol) in NMP (1 mL) was heated at 130° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was then diluted with DCM (5 mL), filtered and concentrated in vacuo. The residue was purified by prep-HPLC to give 6-[4-fluoro-6-(3-fluoroazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (6.2 mg) as a yellow solid and 6-[6-fluoro-4-(3-fluoroazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (6 mg) as a light yellow solid.
6-[4-fluoro-6-(3-fluoroazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.90 (s, 1H), 7.67 (t, 1H), 6.03 (dd, 1H), 5.78 (q, 1H), 5.52-5.56 (m, 1H), 5.48-5.52 (m, 0.5H), 5.31-5.40 (m, 0.5H), 4.58 (br d, 1H), 4.21-4.38 (m, 2H), 3.94-4.10 (m, 2H), 3.43-3.55 (m, 1H), 3.09-3.21 (m, 2H), 1.60 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 396.
6-[6-fluoro-4-(3-fluoroazetidin-1-yl)-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.65 (t, 1H), 5.70 (q, 1H), 5.66 (s, 1H), 5.51-5.55 (m, 0.5H), 5.41 (d, 1H), 5.35-5.40 (m, 0.5H), 4.40-4.54 (m, 1H), 4.20-4.35 (m, 2H), 3.94-4.11 (m, 2H), 3.40-3.56 (m, 1H), 3.07-3.20 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 396.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), azetidin-3-ol hydrochloride (86 mg, 1.18 mmol) and potassium carbonate (162 mg, 1.18 mmol) in NMP (1 mL) was heated at 130° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was then diluted with DCM (5 mL), filtered and concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-ol (6.8 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.64-5.74 (m, 1H), 5.62 (s, 1H), 5.37 (s, 1H), 4.70 (m, 1H), 4.46 (d, 1H), 4.21 (t, 2H), 3.74 (dd, 2H), 3.39-3.54 (m, 1H), 3.05-3.24 (m, 2H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 394.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 3,3-difluoroazetidine hydrochloride (114 mg, 0.88 mmol) and DIPEA (0.15 mL, 0.88 mmol) in NMP (2 mL) was heated at 160° C. with stirring in a microwave reactor for 2 hrs. The resulting reaction mixture was cooled to rt, concentrated in vacuo and purified by prep-HPLC to afford 6-[6-(3,3-difluoroazetidin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (10 mg) as a brown solid and 6-[4-(3,3-difluoroazetidin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (15 mg) as a brown solid.
6-[6-(3,3-difluoroazetidin-1-yl)-4-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.64 (t, 1H), 6.10 (d, 1H), 5.70-5.83 (m, 1H), 5.62 (d, 1H), 4.54 (m, 1H), 4.32 (t, 4H), 3.42-3.54 (m, 1H), 3.06-3.23 (m, 2H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 414.
6-[4-(3,3-difluoroazetidin-1-yl)-6-fluoro-2-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.76 (s, 1H), 5.69-5.75 (m, 1H), 5.50 (s, 1H), 4.44-4.54 (m, 1H), 4.34 (t, 4H), 3.43-3.56 (m, 1H), 3.05-3.25 (m, 2H), 1.58 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 414.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 300 mg, 669 μmol) in dioxane (3 mL) was added tert-butyl 3-oxopiperazine-1-carboxylate (134 mg, 669 μmol), cesium carbonate (436 mg, 1.34 mmol), Xantphos (77.5 mg, 134 μmol) and Pd(OAc)2 (15 mg, 66.9 mol). After being heated at 100° C. with stirring for 20 hrs under argon, the resulting reaction mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by column chromatography (eluting with DCM/MeOH=20/1, v:v) to give tert-butyl 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-oxo-piperazine-1-carboxylate (256 mg) as a yellow solid.
To a solution of tert-butyl 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-oxo-piperazine-1-carboxylate (256 mg) in DCM (5 mL) was TFA (5 mL) and the resulting mixture was stirred for 16 hrs at rt. The resulting reaction mixture was concentrated in vacuo to give the crude 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-2-one (300 mg) which was directly used in the next step without further purification.
To a solution of crude 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-2-one (150 mg) in DCM (3 mL) was added triethylamine (68.2 mg, 674 μmol) and acetic anhydride (34.4 mg, 337 μmol) successively at 0° C. After being slowly warmed to rt and stirred for 2 hrs, the resulting mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 4-acetyl-1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-2-one (12 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.94 (s, 1H), 7.64 (t, 1H), 6.87 (s, 1H), 6.48-6.46 (m, 1H), 5.68-5.66 (m, 1H), 4.55-4.40 (m, 1H), 4.32-4.21 (m, 2H), 3.91-3.77 (m, 4H), 3.55-3.40 (m, 1H), 2.09-2.06 (m, 3H), 1.54 (d, 3H). MS obsd (ESI) [(M+H)+]: 463.
To a solution of 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]piperazin-2-one (the product of step 2 in Example 127, 150 mg) in DCM (3 mL) was added triethylamine (68.2 mg, 674 μmol) and methanesulfonic anhydride (58.7 mg, 337 μmol) successively at 0° C. After being slowly warmed to rt and stirred for 2 hrs at rt, the resulting mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-4-methylsulfonyl-piperazin-2-one (5 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.94 (s, 1H), 7.64 (t, 1H), 6.90 (s, 1H), 6.47 (s, 1H), 5.71-5.62 (m, 1H), 4.50-4.40 (m, 1H), 4.01 (s, 2H), 3.94-3.87 (m, 2H), 3.59-3.56 (m, 2H), 3.51-3.37 (m, 1H), 3.07 (s, 3H), 3.07-3.01 (m, 2H), 1.54 (d, 3H). MS obsd (ESI) [(M+H)+]: 499.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 2-oxa-6-azaspiro[3.3]heptane (87 mg, 0.88 mmol) and potassium carbonate (122 mg, 0.88 mmol) in NMP (1 mL) was heated at 140° C. with stirring in a microwave reactor for 2 hrs. The resulting reaction mixture was diluted with MeOH (4 mL), filtered and purified by prep-HPLC to give 6-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-oxa-6-azaspiro[3.3]heptane (5.4 mg) as a yellow solid and 6-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-oxa-6-azaspiro[3.3]heptane (37 mg) as a yellow solid.
6-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-oxa-6-azaspiro[3.3]heptane, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.65 (t, 1H), 5.98 (m, 1H), 5.76 (q, 1H), 5.47 (m, 1H), 4.84 (s, 4H), 4.50-4.59 (m, 1H), 4.09-4.15 (m, 4H), 3.40-3.51 (m, 1H), 3.09-3.17 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 420.
6-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-oxa-6-azaspiro[3.3]heptane, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.02 (d, 2H), 8.86 (s, 1H), 7.64 (t, 1H), 5.68 (q, 1H), 5.62 (s, 1H), 5.36 (d, 1H), 4.84 (s, 3H), 4.81-4.87 (m, 1H), 4.40-4.50 (m, 1H), 4.08-4.16 (m, 4H), 3.40-3.52 (m, 1H), 3.04-3.22 (m, 2H), 1.55 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 420.
To a solution of 1-Boc-3-(amino)azetidine (1.0 g, 5.81 mmol) in DCM (10 mL) was added acetyl chloride (0.5 mL, 6.97 mmol) at 0° C. After being warmed to rt and stirred for 2 hrs, the resulting mixture was diluted with DCM (80 mL) and washed with water (20 mL) and brine successively. The organic layer were dried over anhydrous Na2SO4 and concentrated in vacuo to give crude tert-butyl 3-acetamidoazetidine-1-carboxylate (500 mg) which was used directly in the next step without any further purification.
To a solution of tert-butyl 3-acetamidoazetidine-1-carboxylate (500 mg, 2.33 mmol) in DCM (3 mL) was added trifluoroacetic acid (1.5 mL, 19.47 mmol) at 0° C. The mixture was warmed to rt with stirring for 12 hrs. The reaction mixture was concentrated in vacuo to give crude N-(azetidin-3-yl)acetamide (250 mg) as a yellow oil which was used directly in the next step.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), N-(azetidin-3-yl)acetamide (101 mg, 0.88 mmol) and potassium carbonate (122 mg, 0.88 mmol) in NMP (1 mL) was heated at 140° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was diluted with DCM (5 mL) and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to give N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidin-3-yl]acetamide (5.2 mg) as a red solid and N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-yl]acetamide (59 mg) as a light red solid.
N-[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidin-3-yl]acetamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.65 (t, 1H), 5.99 (d, 1H), 5.76 (d, 1H), 5.48 (d, 1H), 4.65-4.71 (m, 1H), 4.55 (d, 1H), 4.21-4.31 (m, 2H), 3.74-3.84 (m, 2H), 3.43-3.52 (m, 1H), 3.13 (s, 2H), 1.97 (s, 3H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
N-[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-yl]acetamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.86 (s, 1H), 7.64 (t, 1H), 5.68 (q, 1H), 5.63 (s, 1H), 5.37 (d, 1H), 4.68-4.81 (m, 1H), 4.38-4.54 (m, 1H), 4.21-4.34 (m, 2H), 3.78 (m, 2H), 3.39-3.53 (m, 1H), 3.04-3.20 (m, 2H), 1.95-2.00 (m, 3H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 6-methylpiperazin-2-one (101 mg, 0.88 mmol) and DIPEA (114 mg, 0.88 mmol) in NMP (1 mL) was heated at 200° C. with stirring in a microwave reactor for 2 hrs. The resulting reaction mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-6-methyl-piperazin-2-one (27 mg) as a yellow solid and 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-6-methyl-piperazin-2-one (38 mg) as a yellow solid.
4-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-6-methyl-piperazin-2-one, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.91 (s, 1H), 7.64 (t, 1H), 6.05 (dd, 1H), 5.88 (d, 1H), 5.77 (m, 1H), 4.47-4.58 (m, 1H), 4.27 (dd, 1H), 3.94-4.04 (m, 2H), 3.64-3.75 (m, 1H), 3.44-3.55 (m, 1H), 3.26-3.30 (m, 1H), 3.09-3.20 (m, 2H), 1.59 (dd, 3H), 1.26 (dd, 3H). MS obsd. (ESI+)[(M+H)+]: 435.
4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-6-methyl-piperazin-2-one, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 6.04 (s, 1H), 5.82 (s, 1H), 5.75 (d, 1H), 4.47-4.58 (m, 1H), 3.85-4.10 (m, 2H), 3.80 (m, 1H), 3.66-3.76 (m, 1H), 3.42-3.55 (m, 1H), 3.05-3.25 (m, 3H), 1.58 (d, 3H), 1.28 (dd, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100.0 mg, 0.290 mmol), azetidin-3-ylmethanol (76.8 mg, 0.880 mmol) and DIPEA (0.15 mL, 0.880 mmol) in NMP (2 mL) was heated at 120° C. with stirring in a microwave reactor for 1 hr. The resulting reaction mixture was cooled to rt and concentrated in vacuo. The residue was purified by prep-HPLC to afford [1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-yl]methanol (46.1 mg) as a white solid and [1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidin-3-yl]methanol (14.4 mg) as a white solid.
[1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidin-3-yl]methanol, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.68 (q, 1H), 5.59 (s, 1H), 5.34 (d, 1H), 4.45 (br dd, 1 H), 4.01 (td, 2H), 3.66-3.81 (m, 4H), 3.41-3.53 (m, 1H), 3.04-3.24 (m, 2H), 2.83-2.98 (m, 1H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 408.
[1-[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]azetidin-3-yl]methanol, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.94 (dd, 1H), 5.75 (q, 1H), 5.42 (dd, 1H), 4.51-4.60 (m, 1H), 4.01 (td, 2H), 3.68-3.78 (m, 4H), 3.39-3.51 (m, 1H), 3.04-3.23 (m, 2H), 2.84 (dq, 1H), 1.57 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 408.
To a stirred solution of 5-aminovaleric acid (5.0 g, 42.68 mmol) in dioxane (100 mL) was added a solution of sodium hydroxide (1.79 g, 44.81 mmol) in water (100 mL) and the resulting mixture was stirred at rt for 0.5 hr. Then to the resulting mixture was added Boc2O (9.78 g, 44.81 mmol) slowly. And the reaction mixture was stirred further at rt for 16 hrs, and then concentrated in vacuo. The residue was diluted with H2O (100 mL), then acidified with HCl solution (6 M) to pH=3 and extracted with EA (50 mL) for three times. The combined organic layer was dried over anhydrous Na2SO4 and concentrated in vacuo to give 5-(tert-butoxycarbonylamino)-pentanoic acid (8.7 g) as colorless oil which was used in the next step directly without any further purification.
To a stirred solution of 5-(tert-butoxycarbonylamino)pentanoic acid (1.0 g, 4.6 mmol) and 4-methylmorpholine (0.51 g, 5.06 mmol) in THF (20 mL) was added isobutyl chloroformate (0.69 g, 5.06 mmol) at −20° C. The resulting mixture was warmed to 0° C. and stirred for 1 hr. Then to the resulting mixture was added a solution of NH3 in MeOH (saturated at 0° C.) drop wise. After being stirred at 0° C. for 0.5 hr and warmed up to rt, The resulting mixture was stirred under N2 for 16 hrs at rt, and then concentrated in vacuo. The residue was diluted with H2O (30 mL) and extracted with EA (30 mL) for three times. The combined organic phase was washed with brine (20 mL), dried over anhydrous Na2SO4, concentrated in vacuo to give tert-butyl N-(5-amino-5-oxo-pentyl)carbamate (780 mg) as a white solid, which was used in the next step directly without any further purification.
To a stirred solution of tert-butyl N-(5-amino-5-oxo-pentyl)carbamate (770.0 mg, 3.56 mmol) in EA (15 mL) was added a solution of HCl in dioxane (2.67 mL, 10.68 mmol). The resulting mixture was stirred at rt for 16 hrs and filtered. The filter cake was dried in vacuo to give 5-aminopentanamide (400 mg) as a white solid.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 99.41 mg, 0.290 mmol), 5-aminopentanamide (101.79 mg, 0.880 mmol) and potassium carbonate (121.11 mg, 0.880 mmol) in NMP (2 mL) was heated at 160° C. with stirring for 2 hrs in a microwave reactor. The resulting reaction mixture was cooled to rt and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to afford 5-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]pentanamide (11 mg) as a white solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.83 (s, 1H), 5.64 (q, 1H), 5.54 (d, 1H), 4.40 (dd, 1H), 3.41-3.53 (m, 1H), 3.05-3.23 (m, 4H), 2.22-2.33 (m, 2H), 1.60-1.79 (m, 4H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 437.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), potassium carbonate (207 mg, 1.5 mmol) and 4-aminotetrahydro-2H-thiopyran 1,1-dioxide (224 mg, 1.5 mmol) in NMP (5 mL) was heated at 130° C. with stirring for 15 hrs. After being cooled to rt, the mixture was poured into water (50 mL) and extracted with DCM (75 mL) twice. The organic layers were combined, then washed with water and brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide N-(1,1-dioxothian-4-yl)-2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (2 mg) as a white solid. 1H NMR (400 MHz, CDCl3) δ ppm: 8.93 (d, 2H), 8.76 (s, 1H), 7.54 (m, 1H), 5.78 (s, 1H), 5.53-5.60 (m, 1H), 5.50-5.53 (m, 1H), 4.26-4.36 (m, 1H), 3.68 (m, 1H), 3.32-3.43 (m, 1H), 3.11-3.17 (m, 2H), 2.94-3.09 (m, 4H), 2.23 (m, 2H), 1.93-2.14 (m, 2H), 1.46 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 470
To a stirred solution of 3-Boc-aminoazetidine hydrochloride (2.0 g, 9.58 mmol) and triethylamine (4.01 mL, 28.75 mmol) in DCM (40 mL) was added acetyl chloride (1.02 mL, 14.38 mmol) slowly at 0° C. and the resulting mixture was stirred at 0° C. for 1 hr. The resulting reaction mixture was diluted with DCM (20 mL), then washed with H2O (10 mL) and brine (10 mL) successively. The organic phase was dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by the flash column (eluting with PE/EA=5/1, v:v) to afford tert-butyl N-(1-acetylazetidin-3-yl)carbamate (1.85 g) as a yellow oil.
A mixture of tert-butyl N-(1-acetylazetidin-3-yl)carbamate (1.0 g, 4.67 mmol) and TFA (2.0 mL, 25.96 mmol) in DCM (10 mL) was stirred at 20° C. for 1 hr. The resulting reaction mixture was concentrated in vacuo to give crude 1-(3-aminoazetidin-1-yl)ethanone (450 mg) which was used directly in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 201.13 mg, 0.880 mmol), 1-(3-aminoazetidin-1-yl)ethanone (100.0 mg, 0.290 mmol) and potassium carbonate (203.05 mg, 1.47 mmol) in NMP (0.500 mL) was heated at 170° C. with stirring in a microwave reactor for 2 hrs. The resulting reaction mixture was filtered and concentrated in vacuo. The residue was purified by prep-HPLC to afford 1-[3-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]azetidin-1-yl]ethanone (6.8 mg) as yellow solid and 1-[3-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]azetidin-1-yl]ethanone (12.2 mg) as a yellow solid.
1-[3-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]amino]azetidin-1-yl]ethanone, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.65 (t, 1H), 5.94 (d, 1H), 5.70-5.82 (m, 1H), 5.60 (dd, 1H), 4.54-4.67 (m, 2H), 4.44-4.53 (m, 1H), 4.34 (q, 1H), 4.05-4.13 (m, 1H), 3.90 (dt, 1H), 3.41-3.54 (m, 1H), 3.06-3.21 (m, 2H), 1.91 (s, 3H), 1.58 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
1-[3-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]azetidin-1-yl]ethanone, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.78 (s, 1H), 5.66 (q, 1H), 5.51 (d, 1H), 4.59 (br t, 1H), 4.32-4.47 (m, 3H), 4.01 (dd, 1H), 3.77-3.87 (m, 1H), 3.42-3.55 (m, 1H), 3.06-3.24 (m, 2H), 1.90 (d, 3H), 1.56 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 435.
To a mixture of 5-aminovaleric acid (10.0 g, 0.085 mol) and an aqueous solution of NaOH (50 mL, 2.0 M) which was cooled to 0° C., were added CbzCl (16.0 g, 0.094 mol) and an aqueous solution of NaOH (50 mL, 2.0 M) simultaneously. The resulting mixture was warmed to rt and stirred for 0.5 hrs, then extracted with EA (300 mL) for three times. The combined organic phase was washed with brine (10 mL), dried over anhydrous Na2SO4 and concentrated in vacuo to give crude 5-(benzyloxycarbonylamino)-pentanoic acid (20.0 g) as a white solid, which was used directly in the next step without any further purification.
To a solution of 5-(benzyloxycarbonylamino)-pentanoic acid (2.0 g, 7.96 mmol) in DMF (40 mL) was added NaH (60% in oil, 0.57 g, 23.88 mmol) at 0° C. The mixture was stirred at 0° C. for 1 hr and then to the resulting mixture was added MeI (4.88 mL, 47.76 mmol) at 0° C. The resulting mixture was warmed to rt and stirred for 2 hrs, then quenched with saturated NH4Cl (150 mL) and extracted with EA (100 mL) for three times. The combined organic phase was washed with brine, dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo and the residue was purified by column (eluting with PE/EA=10/1, v:v) to give methyl 5-[benzyloxycarbonyl(methyl)amino]pentanoate (2.2 g) as a yellow oil.
To a solution of methyl 5-[benzyloxycarbonyl(methyl)amino]pentanoate (2.2 g, 7.88 mmol) in THF (22 mL) was added a solution of LiOH (1.32 g, 31.5 mmol) in water (10 mL) at 0° C. After being heated at 60° C. with stirring for 1 hr and cooled to rt, the resulting mixture was concentrated in vacuo until the volume was around 25 mL, and the residue was acidified to pH=5 with 1M HCl and extracted with EA (100 mL) for three times. The combined organic phase was dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo to give 5-[benzyloxycarbonyl-(methyl)amino]pentanoic acid (1.6 g) as a yellow oil, which was used directly in the next step without any further purification.
To a solution of 5-[benzyloxycarbonyl(methyl)amino]pentanoic acid (1.5 g, 5.65 mmol) in DMF (20 mL) was added HATU (4.3 g, 11.31 mmol), NH4Cl (0.91 g, 16.96 mmol) and DIPEA (2.95 mL, 16.96 mmol) at rt. The resulting mixture was stirred for 3 hrs at rt, then diluted with water (100 mL) and extracted with EA (100 mL) for three times. The combined organic phase was dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to give benzyl N-(5-amino-5-oxo-pentyl)-N-methyl-carbamate (1.2 g) as a light yellow oil.
To a solution of benzyl N-(5-amino-5-oxo-pentyl)-N-methyl-carbamate (1.2 g, 4.54 mmol) in MeOH (20 mL) was added Pd/C (10%, 300 mg) and Boc2O (1.98 g, 9.08 mmol). The resulting mixture was degassed, charged with H2 (15 psi), and then stirred at rt for 2 hrs. The mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by column (eluting with PE/EA=5/1, v:v) to give tert-butyl N-(5-amino-5-oxo-pentyl)-N-methyl-carbamate (0.9 g) as a colorless oil.
To a solution of tert-butyl N-(5-amino-5-oxo-pentyl)-N-methyl-carbamate (0.5 g, 2.17 mmol) in EA (5 mL) was added a solution of HCl in EA (3.0 mL, 4 M). The resulting mixture was stirred for 2 hrs at rt and then concentrated in vacuo to give 5-(methylamino)pentanamide (0.4 g) as a yellow gum, which was used directly in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100.0 mg, 0.290 mmol), K2CO3 (203.05 mg, 1.47 mmol) and 5-(methylamino)pentanamide (45.9 mg, 0.350 mmol) in DMSO (2 mL) was heated at 150° C. with stirring for 1 hr in a microwave reactor. The resulting reaction mixture was diluted with DCM (100 mL), washed with water (50 mL) and brine (50 mL), dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 5-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-methyl-amino]pentanamide (8 mg) as a yellow solid and 5-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-methyl-amino]pentanamide (5.5 mg) as a yellow solid.
5-[[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-methyl-amino]pentanamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.90 (s, 1H), 7.66 (t, 1H), 5.84 (s, 1H), 5.68-5.73 (m, 1H), 5.66 (s, 1H), 4.67 (br s, 2H), 4.47 (br dd, 1H), 3.48-3.53 (m, 1H), 3.41-3.47 (m, 2H), 3.13-3.25 (m, 2H), 3.03 (s, 3H), 2.24-2.32 (m, 2H), 1.67 (br d, 4H), 1.59 (d, 3H). MS obsd (ESI) [(M+H)]: 451.
5-[[4-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-methyl-amino]pentanamide, 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.91 (s, 1H), 7.66 (t, 1H), 5.91 (d, 1H), 5.78 (q, 1H), 5.65 (d, 1H), 4.48-4.69 (m, 2H), 4.45-4.56 (m, 1H), 3.58 (br s, 2H), 3.46-3.54 (m, 1H), 3.12-3.23 (m, 2H), 3.01 (s, 3H), 2.23-2.30 (m, 2H), 1.64-1.72 (m, 4H), 1.60 (d, 3H). MS obsd (ESI) [(M+H)+]: 451.
To a solution of 5-(benzyloxycarbonylamino)pentanoic acid (3.0 g, 11.94 mmol) in DMF (50 mL) was added NaH (60% in oil, 1.19 g, 29.85 mmol) at 0° C. The resulting mixture was stirred for 1 hr at 0° C. To the mixture was added (bromomethyl)-cyclopropane (2.9 mL, 29.85 mmol) at 0° C. After being heated at 50° C. with stirring for 2 hrs, the resulting mixture was quenched with saturated NH4Cl (100 mL) and extracted with EA (100 mL) twice. The combined organic phase was washed with brine, dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo to give crude 5-[benzyloxycarbonyl(cyclopropylmethyl)amino]pentanoic acid (4.9 g) as a light yellow oil.
To a solution of 5-[benzyloxycarbonyl(cyclopropylmethyl)amino]pentanoic acid (4.9 g, 9.63 mmol) in DMF (30 mL) was added K2CO3 (3.99 g, 28.88 mmol) and MeI (5.9 mL, 57.77 mmol). The resulting mixture was stirred for 2 hrs at rt, then diluted with saturated aqueous NH4Cl (100 mL) and extracted with EA (80 mL) for three times. The combined organic phase was washed with brine (100 mL), dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo and the residue was purified by column (eluting with PE/EA=5/1, v:v) to give methyl 5-[benzyloxycarbonyl(cyclopropylmethyl)amino]-pentanoate (2 g) as a light yellow oil.
To a solution of methyl 5-[benzyloxycarbonyl(cyclopropylmethyl)amino]-pentanoate (2.9 g, 9.08 mmol) in THF (20 mL) was added a solution of LiOH monohydrate (1.52 g, 36.32 mmol) in water (10 mL) drop wise at 0° C. After being heated at 60° C. with stirring for 1 hr, the resulting mixture was cooled to rt and concentrated in vacuo until the volume is around 25 mL. The residue was acidified to pH=5 with 1M HCl and extracted with EA (100 mL) twice. The combined organic phase was dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo to give 5-[benzyloxycarbonyl-(cyclopropylmethyl)amino]pentanoic acid (2.6 g) as a yellow oil, which was used directly in the next step without any further purification.
To a solution of 5-[benzyloxycarbonyl-(cyclopropylmethyl)amino]pentanoic acid (2.5 g, 8.19 mmol) in DMF (25 mL) was added HATU (4.67 g, 12.28 mmol), NH4Cl (1.31 g, 24.56 mmol) and DIPEA (4.28 mL, 24.56 mmol). After being stirred for 3 hrs at rt, the resulting mixture was diluted with water (100 mL) and extracted with EA (100 mL) for three times. The combined organic phase was dried over anhydrous Na2SO4 and filtered. The filtrate was concentrated in vacuo and the residue was purified by prep-HPLC to give benzyl N-(5-amino-5-oxo-pentyl)-N-(cyclopropylmethyl)-carbamate (1.6 g) as a light yellow oil.
To a solution of benzyl N-(5-amino-5-oxo-pentyl)-N-(cyclopropylmethyl)-carbamate (1.6 g, 5.26 mmol) in methanol (20 mL) was added Pd/C (1.12 g, 10% wt) and Boc2O (2.29 g, 10.51 mmol). The resulting mixture was degassed, charged with H2 (15 psi) and stirred at rt for 2 hrs. The resulting reaction mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by column (eluting with PE/EA=5/1, v:v) to give tert-butyl N-(5-amino-5-oxo-pentyl)-N-(cyclopropylmethyl)-carbamate (1.4 g) as a colorless oil.
Tert-butyl N-(5-amino-5-oxo-pentyl)-N-(cyclopropylmethyl)-carbamate (400 mg, 1.48 mmol) was stirred in a solution of HCl in MeOH (4 mL, 1M) at rt for 2 hrs. The resulting reaction mixture was basified by basic resin and filtered. The filtrate was concentrated in vacuo to give 5-(cyclopropylmethyl-amino)pentanamide (300 mg) as a yellow oil which was used directly in the next step without any further purification.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), 5-(cyclopropylmethylamino)pentanamide (150 mg, 0.88 mmol) and K2CO3 (122 mg, 0.88 mmol) in DMSO (1 mL) was heated at 160° C. with stirring for 1 hr in a microwave reactor. The resulting reaction mixture was diluted with EA (30 mL), then washed with H2O (20 mL) and brine (20 mL) successively, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to give 5-[cyclopropylmethyl-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]amino]pentanamide (5 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.64 (t, 1H), 5.86 (s, 1H), 5.61-5.74 (m, 2H), 4.45 (d, 1H), 3.54-3.55 (m, 1H), 3.42-3.55 (m, 2H), 3.21-3.29 (m, 2H), 3.07-3.20 (m, 2H), 2.27 (s, 2H), 1.67 (br. s, 4H), 1.58 (d, 3H), 1.07 (d, 1H), 0.57 (d, 2H), 0.31 (d, 2H). MS obsd. (ESI+) [(M+H)+]: 491.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 100 mg, 0.29 mmol), azetidine-3-carbonitrile (72 mg, 0.88 mmol) and DIPEA (114 mg, 0.88 mmol) in NMP (1 mL) was heated at 160° C. with stirring for 1 hr in a microwave reactor. The resulting reaction mixture was diluted with MeOH (4 mL), filtered and purified by prep-HPLC to afford 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]azetidine-3-carbonitrile (8 mg) as a red solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.86 (s, 1H), 7.64 (t, 1H), 5.63-5.76 (m, 2H), 5.42 (s, 1H), 4.41-4.52 (m, 1H), 4.28 (m, 2H), 4.08-4.19 (m, 2H), 3.81 (m, 1H), 3.40-3.55 (m, 1H), 3.03-3.23 (m, 2H), 1.57 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 403.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 150 mg, 335 μmol) in dioxane (3 mL) was added methyl 5-oxopyrrolidine-3-carboxylate (95.8 mg, 669 μmol), cesium carbonate (218 mg, 669 μmol), Xantphos (38.7 mg, 66.9 μmol) and Pd(OAc)2 (7.51 mg, 33.5 mol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under argon. After being cooled to rt, the resulting reaction mixture was filtered and the filtrate was concentrated in vacuo to give crude methyl 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-5-oxo-pyrrolidine-3-carboxylate (210 mg) which was directly used in the next step without any further purification.
To a solution of the crude methyl 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-5-oxo-pyrrolidine-3-carboxylate (210 mg) in a mixture solvent of MeOH (3 mL) and water (1 mL) was added lithium hydroxide monohydrate (49.4 mg) at rt. The resulting mixture was stirred for 2 hrs at rt, then acidified with acetic acid and concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4, 3-d]pyrimidin-6-yl)-4-pyridyl]-5-oxo-pyrrolidine-3-carboxylic acid (18 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.95 (s, 1H), 7.64 (t, 1H), 6.90 (d, 1H), 6.84 (d, 1H), 5.73-5.65 (m, 1H), 4.53-4.43 (m, 1H), 4.15-4.07 (m, 1H), 4.07-3.99 (m, 1H), 3.53-3.44 (m, 1H), 3.13-2.98 (m, 2H), 2.88-2.71 (m, 3H), 1.52 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 450.
A mixture of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), thiomorpholine 1,1-dioxide (203 mg, 1.5 mmol) and potassium carbonate (207 mg, 1.5 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs under nitrogen. After being cooled to rt, the resulting mixture was diluted with water (25 mL) and extracted with DCM (75 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide 4-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-1,4-thiazinane 1,1-dioxide (2 mg) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.90 (s, 1H), 7.63 (t, 1H), 6.20 (s, 1H), 6.03 (s, 1H), 5.73 (q, 1H), 4.49 (br dd, 1H), 3.94 (br s, 4H), 3.35-3.49 (m, 1H), 3.09-3.18 (m, 4H), 2.90-3.08 (m, 2H), 1.50 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 456.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), azetidine hydrochloride (94 mg, 1.0 mmol) and potassium carbonate (207 mg, 1.5 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs under nitrogen. After being cooled to rt, the resulting mixture was diluted with water (25 mL) and extracted with DCM (75 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide 6-[2-(azetidin-1-yl)-6-fluoro-4-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (85 mg) as a light yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.90 (s, 1H), 7.67 (t, 1H), 5.97 (t, 1H), 5.61 (s, 1H), 5.36 (q, 1H), 4.08-4.19 (m, 1H), 4.01 (t, 4H), 3.55-3.64 (m, 1H), 3.14-3.31 (m, 2H), 2.34-2.42 (m, 2H), 1.61 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 378.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), 3-methoxyazetidine hydrochloride (124 mg, 1.0 mmol) and potassium carbonate (207 mg, 1.5 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs under nitrogen. After being cooled to rt, the resulting mixture was diluted with water (25 mL) and extracted with DCM (75 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide 6-[2-fluoro-6-(3-methoxyazetidin-1-yl)-4-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (100 mg) as a white solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.89 (s, 1H), 7.66 (t, 1H), 5.99 (t, 1H), 5.66 (s, 1H), 5.36 (d, 1H), 4.34 (tt, 1H), 4.08-4.21 (m, 3H), 3.81 (dd, 2H), 3.53-3.65 (m, 1H), 3.35 (s, 3H), 3.13-3.27 (m, 2H), 1.61 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 408.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), 3,3-difluoroazetidine hydrochloride (156 mg, 1.0 mmol) and potassium carbonate (207 mg, 1.5 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs under nitrogen. After being cooled to rt, the resulting mixture was diluted with water (25 mL) and extracted with DCM (75 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide 6-[2-(3,3-difluoroazetidin-1-yl)-6-fluoro-4-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (35 mg) as a white solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.05 (d, 2H), 8.89 (s, 1H), 7.67 (t, 1H), 6.10 (t, 1H), 5.84 (s, 1H), 5.39 (q, 1H), 4.34 (t, 4H), 4.08-4.28 (m, 1H), 3.56-3.69 (m, 1H), 3.11-3.30 (m, 2H), 1.62 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 414.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), 3-fluoroazetidine hydrochloride (110 mg, 1.0 mmol) and potassium carbonate (207 mg, 1.5 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs under nitrogen. After being cooled to rt, the resulting mixture was diluted with water (25 mL) and extracted with DCM (75 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide 6-[2-fluoro-6-(3-fluoroazetidin-1-yl)-4-pyridyl]-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (75 mg) as a white solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 8.93 (d, 2H), 8.78 (s, 1H), 7.55 (t, 1H), 5.91 (t, 1H), 5.59 (s, 1H), 5.19-5.31 (m, 2H), 4.12-4.23 (m, 2H), 3.87-4.07 (m, 3H), 3.33-3.56 (m, 1H), 3.02-3.20 (m, 2H), 1.49 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 396.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added 2-oxa-6-azaspiro[3.4]octan-7-one (42.5 mg, 335 μmol), cesium carbonate (145 mg, 446 μmol), Xantphos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under argon. After being cooled to rt, the reaction mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 7-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-2-oxa-7-azaspiro[3.4]octan-6-one (12 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.96 (s, 1H), 7.64 (t, 1H), 6.88 (s, 1H), 6.84 (s, 1H), 5.74-5.64 (m, 1H), 4.65-4.58 (m, 4H), 4.50-4.43 (m, 1H), 4.22 (s, 2H), 3.55-3.45 (m, 1H), 3.13-3.03 (m, 2H), 2.98 (s, 2H), 1.53 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 448.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added 3-methylpyrrolidin-2-one (33.2 mg, 335 μmol), cesium carbonate (145 mg, 446 mol), Xantphos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under argon. After being cooled to rt, the resulting reaction mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-3-methyl-pyrrolidin-2-one (15 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.95 (s, 1H), 7.64 (t, 1H), 6.98 (d, 1H), 6.81 (d, 1H), 5.72-5.66 (m, 1H), 4.50-4.39 (m, 1H), 3.91-3.72 (m, 2H), 3.55-3.44 (m, 1H), 3.13-2.98 (m, 2H), 2.78-2.66 (m, 1H), 2.38-2.27 (m, 1H), 1.76-1.64 (m, 1H), 1.53 (d, 3H), 1.17 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 420.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added 4-(hydroxymethyl)pyrrolidin-2-one (38.5 mg, 335 μmol), cesium carbonate (145 mg, 446 μmol), XantPhos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under argon. After being cooled to rt, the resulting reaction mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4, 3-d]pyrimidin-6-yl)-4-pyridyl]-4-(hydroxymethyl) pyrrolidin-2-one (12 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.95 (s, 1H), 7.64 (t, 1H), 6.90-6.87 (m, 1H), 6.87-6.85 (m, 1H), 5.73-5.63 (m, 1H), 4.96-4.84 (m, 1H), 4.54-4.39 (m, 1H), 4.01-3.90 (m, 1H), 3.72-3.64 (m, 1H), 3.53-3.41 (m, 3H), 3.12-2.99 (m, 2H), 2.72-2.63 (m, 1H), 2.60-2.53 (m, 1H), 2.41-2.33 (m, 1H), 1.53 (d, 3H). MS obsd. (ESI+)[(M+H)+]: 436.
To a stirring solution of 6-(4,6-difluoro-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 600.0 mg, 1.76 mmol) in DMA (10 mL) was added K2CO3 (3.66 g, 26.44 mmol) and methylamine hydrochloride (1.79 g, 26.44 mmol). The resulting mixture was heated at 40° C. with stirring for 12 hrs and then filtrated. The filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (280 mg) as a yellow solid.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (20 mg, 0.06 mmol) in DCM (0.5 mL) was added acetyl chloride (13.4 mg, 0.17 mmol) and DIPEA (22.07 mg, 0.17 mmol) at rt. The resulting mixture was stirred at rt for 1 h, then diluted with MeOH (1 mL) and concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-acetamide (18 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.65 (t, 1H), 6.76 (s, 1H), 6.24 (s, 1H), 5.77 (q, 1H), 4.55-4.61 (m, 1H), 3.51-3.64 (m, 1H), 3.29 (s, 3H), 3.14-3.22 (m, 2H), 2.07 (s, 3H), 1.62 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 394.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (the product of step 1 in Example 154, 20 mg, 0.06 mmol) in DCM (0.5 mL) was added propionyl chloride (22.1 mg, 0.17 mmol) and DIPEA (22.07 mg, 0.17 mmol) at rt. The resulting mixture was stirred for 1 h at rt, then diluted with MeOH (1 mL) and concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-propanamide (3 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.65 (t, 1H), 6.75 (s, 1H), 6.23 (s, 1H), 5.76 (q, 1H), 4.58 (d, 1H), 3.49-3.66 (m, 1H), 3.28 (s, 3H), 3.14-3.22 (m, 2H), 2.35 (q, 2H), 1.62 (d, 3H), 1.09 (t, 3H). MS obsd. (ESI+) [(M+H)+]: 408.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (the product of step 1 in Example 154, 20 mg, 0.06 mmol) in DCM (0.5 mL) was added cyclopropanecarbonyl chloride (17.9 mg, 0.17 mmol) and DIPEA (22.07 mg, 0.17 mmol) at rt. The resulting mixture was stirred at rt for 1 hr, then diluted with MeOH (1 mL) and concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-cyclopropanecarboxamide (6 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.64 (t, 1H), 6.80 (s, 1H), 6.28 (s, 1H), 5.77 (q, 1H), 4.52-4.63 (m, 1H), 3.50-3.67 (m, 1H), 3.13-3.23 (m, 2H), 1.67-1.78 (m, 1H), 1.62 (d, 3H), 0.94-1.01 (m, 2H), 0.74-0.85 (m, 2H). MS obsd. (ESI+) [(M+H)+]: 420.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added imidazolidin-2-one (28.8 mg, 335 μmol), cesium carbonate (145 mg, 446 mol), XantPhos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). The resulting mixture was heated at 100° C. with stirring for 20 hrs under argon. After being cooled to rt, the reaction mixture was filtered and the filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 1-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4, 3-d]pyrimidin-6-yl)-4-pyridyl]imidazolidin-2-one (8 mg) as a light yellow solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 8.99 (d, 2H), 8.94 (s, 1H), 7.64 (t, 1H), 7.37 (s, 1H), 6.76 (s, 1H), 6.71 (s, 1H), 5.71-5.62 (m, 1H), 4.45-4.38 (m, 1H), 3.96-3.86 (m, 2H), 3.51-3.41 (m, 3H), 3.12-2.99 (m, 2H), 1.51 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 407.
A mixture of 6-(2,6-difluoro-4-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (one product of step 7 in Example 1, 170 mg, 0.5 mmol), 2-oxa-6-azaspiro[3.3]heptane hemioxalate (144 mg, 1.0 mmol) and potassium carbonate (207 mg, 1.5 mmol) in NMP (5 mL) was heated at 110° C. with stirring for 15 hrs under nitrogen. After being cooled to rt, the resulting mixture was diluted with water (25 mL) and extracted with DCM (75 mL) twice. The combined organic layer was washed with brine, dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was purified by prep-HPLC to provide 6-[6-fluoro-4-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-2-pyridyl]-2-oxa-6-azaspiro[3.3]heptane (68 mg) as a white solid. 1H NMR (400 MHz, DMSO-d6) δ ppm: 9.00 (d, 2H), 8.87 (s, 1H), 7.64 (t, 1H), 5.99 (s, 1H), 5.67 (s, 1H), 5.38 (q, 1H), 4.68-4.75 (m, 4H), 4.00-4.13 (m, 5H), 3.43-3.57 (m, 1H), 2.94-3.14 (m, 2H), 1.49 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 420.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (the product of step 1 in Example 154, 30 mg, 0.09 mmol) in pyridine (0.5 mL) was added methanesulfonyl chloride (29 mg, 0.26 mmol) at rt. The mixture was stirred for 12 hrs at rt and then concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-methanesulfonamide (10 mg) as a red solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.90 (s, 1H), 7.65 (t, 1H), 6.73 (s, 1H), 6.37 (s, 1H), 5.69-5.81 (m, 1H), 4.50-4.59 (m, 1H), 3.50-3.61 (m, 1H), 3.36 (s, 3H), 3.13-3.23 (m, 1H), 3.12-3.13 (m, 1H), 2.96-3.06 (m, 3H), 1.61 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 430.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (the product of step 1 in Example 154, 40 mg, 0.11 mmol) in pyridine (0.5 mL) was added cyclopropanesulfonyl chloride (48 mg, 0.34 mmol) at rt. The resulting mixture was stirred for 12 hrs at rt and then concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-cyclopropanesulfonamide (23 mg) as a light green solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (br d, 2H), 8.91 (s, 1H), 7.65 (t, 1H), 6.76 (s, 1H), 6.39 (s, 1H), 5.74 (q, 1H), 4.54 (m, 1H), 3.48-3.65 (m, 1H), 3.38 (s, 3H), 3.10-3.21 (m, 2H), 2.62-2.78 (m, 1H), 1.61 (d, 3H), 0.96-1.14 (m, 4H). MS obsd. (ESI+) [(M+H)+]: 456.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (the product of step 1 in Example 154, 20 mg, 0.06 mmol) in pyridine (0.5 mL) was added benzenesulfonyl chloride (0.03 mL, 0.260 mmol) at rt. The resulting mixture was stirred at rt for 1 hr and then concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-benzenesulfonamide (27 mg) as a light yellow solid. H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.88 (s, 1H), 7.61-7.74 (m, 4H), 7.50-7.60 (m, 1H), 7.50-7.60 (m, 1H), 6.53 (s, 1H), 6.13 (s, 1H), 5.64 (q, 1H), 4.49 (s, 1H), 4.46 (s, 1H), 3.43-3.58 (m, 1H), 3.27 (s, 3H), 3.06-3.16 (m, 2H), 1.55 (d, 3H). MS obsd. (ESI) [(M+H)+]: 492.
To a solution of 6-(6-fluoro-4-iodo-2-pyridyl)-5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidine (the product of step 1 in Example 96, 100 mg, 223 μmol) in dioxane (3 mL) was added 1,2-thiazinane1,1-dioxide (30.2 mg, 223 μmol), cesium carbonate (145 mg, 446 μmol), Xantphos (25.8 mg, 44.6 μmol) and Pd(OAc)2 (5.01 mg, 22.3 μmol). After being heated at 100° C. with stirring for 20 hrs under argon and cooled to rt, the resulting reaction mixture was filtered. The filtrate was concentrated in vacuo. The residue was purified by prep-HPLC to give 2-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]thiazinane1,1-dioxide (15 mg) as a light yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.06 (d, 2H), 8.92 (s, 1H), 7.67 (t, 1H), 6.67 (s, 1H), 6.26 (s, 1H), 5.75-5.73 (m, 1H), 4.65-4.50 (m, 1H), 3.90-3.80 (m, 2H), 3.65-3.50 (m, 1H), 3.35-3.25 (m, 2H), 3.26-3.15 (m, 2H), 2.40-2.25 (m, 2H), 2.01-1.85 (m, 2H), 1.62 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 456.
To a solution of 2-fluoro-N-methyl-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)pyridin-4-amine (the product of step 1 in Example 154, 20 mg, 0.06 mmol) in DCM (0.5 mL) was added benzoylchloride (17.9 mg, 0.17 mmol) and DIPEA (22.07 mg, 0.17 mmol) at rt. The resulting mixture was stirred for 1 h at rt and diluted with MeOH (1 mL). The resulting mixture was concentrated in vacuo. The residue was purified by prep-HPLC to give N-[2-fluoro-6-(5-methyl-2-pyrimidin-2-yl-7,8-dihydro-5H-pyrido[4,3-d]pyrimidin-6-yl)-4-pyridyl]-N-methyl-benzamide (12 mg) as a yellow solid. 1H NMR (400 MHz, Methanol-d4) δ ppm: 9.03 (d, 2H), 8.81 (s, 1H), 7.64 (t, 1H), 7.38-7.44 (m, 2H), 7.26-7.37 (m, 2H), 7.26-7.37 (m, 1H), 6.36 (s, 1H), 6.16 (s, 1H), 5.45 (q, 1H), 4.34 (m, 1H), 3.49 (s, 3H), 3.34-3.43 (m, 1H), 2.90-3.10 (m, 2H), 1.40 (d, 3H). MS obsd. (ESI+) [(M+H)+]: 456.
HBV Cell Line
HepG2.2.15 cells (Acs et al. Proc Natl Acad Sci USA, 84, (1987), 4641-4), a constitutively HBV-expressing cell line were cultured in DMEM+Glutamax-I medium (Invitrogen, Carlsbad, Calif., USA), supplemented with 10% fetal bovine serum (Invitrogen) and G418 (Invitrogen) at a final concentration of 200 mg/L and maintained in 5% CO2 at 37° C.
HBsAg Assay
HepG2.2.15 cells were seeded in duplicate into white, 96-well plates at 1.5×104 cells/well. The cells were treated with a three-fold serial dilution series of the compounds in DMSO. The final DMSO concentration in all wells was 1% and DMSO was used as no drug control.
The HBsAg chemiluminescence immunoassay (CLIA) kit (Autobio Diagnostics Co., Zhengzhou, China, Catalog number: CL0310-2) was used to measure the levels of secreted HBV antigens semi-quantitatively. For the detection 50 μL/well culture supernatant was used and HBsAg was quantified using HBsAg chemiluminescence immunoassay (CLIA) kit (Autobio Diagnostics Co., Zhengzhou, China, Catalog number: CL0310-2), 50 μL of the supernatant was transferred to the CLIA assay plate and 50 μL of enzyme conjugate reagent was added into each well. The plates were sealed and gently agitated for 1 hour at room temperature. The supernatant-enzyme-mixture was discarded and wells were washed 6 times with 300 μL of PBS.
The residual liquid was removed by plating the CLIA plate right side down on absorbent tissue paper. 25 μL of substrates A and B were added to each well. Luminance was measured using a luminometer (Mithras LB 940 Multimode Microplate Reader) after 10 minutes incubation. Dose-response curves were generated and the IC50 value was extrapolated by using the E-WorkBook Suite (ID Business Solutions Ltd., Guildford, UK). The IC50 was defined as the compound concentration (or conditioned media log dilution) at which HBsAg secretion was reduced by 50% compared to the no drug control.
The compounds according to formula I were tested for their capacity to inhibit HBsAg as described herein. The Examples were tested in the above assay and found to have IC50 below 50 μM. Particular compounds of formula I were found to have IC50 below 0.50 μM. More Particular compounds of formula I were found to have IC50 below 0.100 μM. Results of HBsAg assay are given in Table 1.
HBV DNA Assay
The assay employs real-time qPCR (TaqMan) to directly measure extracellular HBV DNA copy number in the cell supernatant. HepG2.2.15 cells were plated in 96-well microtiter plates before treatment with complete medium (DMEM, Glutamax, 10% FBS, 1% Penicillin/Streptomycin, 250 μg/mL Genetycin, final DMSO concentration is 1%/o). Only the interior wells were utilized to reduce “edge effects” observed during cell culture, the exterior wells were filled with complete medium to help minimize sample evaporation. The HepG2.2.15 cells were treated 1 h later with various concentrations of a test compound in duplicate (top concentration used at 5 μM, 2 μM or 0.5 μM according to the HBsAg IC50 observed, with ⅓ successive dilutions (total of 10 dilutions). Six days following the initial administration of the test compound, the cell culture supernatant was collected; DNA extraction was performed by automated system (Magnapure) and then used in a real-time qPCR/TaqMan assay to determine HBV DNA copy numbers. Antiviral activity was calculated from the reduction in HBV DNA levels (IC50).
The compounds of the present invention were tested for their capacity to inhibit HBV DNA as described herein. The Examples were tested in the above assay and found to have IC50 below 50 μM. Results of HBV DNA assay are given in Table 2.
| Number | Date | Country | Kind |
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| PCT/CN2016/087607 | Jun 2016 | WO | international |
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| 7977342 | Simmen et al. | Jul 2011 | B2 |
| 9434725 | Do et al. | Sep 2016 | B2 |
| 9487533 | Castanedo et al. | Nov 2016 | B2 |
| Number | Date | Country |
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| 2008-514678 | May 2008 | JP |
| 2010-512337 | Apr 2010 | JP |
| 2015-521645 | Jul 2015 | JP |
| 02064574 | Aug 2002 | WO |
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| Entry |
|---|
| Geng et al., “Small-molecule inhibitors for the treatment of hepatitis B virus documented in patents” Mini Reviews in Medicinal Chemistry 13(5):749-776 ( 2013). |
| International Preliminary Report on Patentability (IPRP) for PCT/EP2017/065690 dated Jan. 1, 2019. |
| International Search Report for PCT/EP2017/065690 dated Jul. 31, 2017. |
| Number | Date | Country | |
|---|---|---|---|
| 20190241559 A1 | Aug 2019 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/EP2017/065690 | Jun 2017 | US |
| Child | 16227076 | US |
