Patent Application
20230277533
The content of the electronically submitted sequence listing (Name 4195_012PC03_Seglisting_ST25.txt; Size: 7,446 bytes; and Date of Creation: Feb. 11, 2021) is incorporated herein by reference in its entirety.
The present disclosure provides therapeutic combinations of ubiquitin-specific-processing protease 1 (USP1) inhibitors and Poly (ADP-ribose) polymerase (PARP) inhibitors. Methods of treating cancers comprising administering the combinations are also provided.
Ubiquitin is a small (76 amino acid) protein that is post-transcriptionally attached to target proteins. The consequence of ubiquitination is determined by the number and linkage topology of ubiquitin molecules conjugated to the target protein. For example, proteins exhibiting lysine 48-linked poly-ubiquitin chains are generally targeted to the proteasome for degradation, while mono-ubiquitination or poly-ubiquitin chains linked through other lysines regulate non-proteolytic functions, such as cell cycle regulation, DNA damage repair, transcription, and endocytosis. Ubiquitination is a reversible process, and enzymes called deubiquitinases remove ubiquitin from target proteins.
USP1 is a deubiquitinase that plays a role in DNA damage repair. USP1 interacts with UAF1 (USP1-associated factor 1) to form a complex that is required for the deubiquitinase activity. The USP1/UAF1 complex deubiquitinates mono-ubiquitinated PCNA (proliferating cell nuclear antigen) and mono-ubiquitinated FANCD2 (Fanconi anemia group complementation group D2), which are proteins that play important functions in translesion synthesis (TLS) and the Fanconi anemia (FA) pathway, respectively. The USP1/UAF1 complex also deubiquitinates Fanconi anemia complementation group I (FANCI). These two pathways are essential for repair of DNA damage induced by DNA cross-linking agents, such as cisplatin and mitomycin C (MMC).
The Poly (ADP-ribose) polymerase (PARP) family of enzymes plays roles in DNA repair and genome integrity. PARP is critical for single stranded break repair and base excision repair pathways. A key enzymatic activity is to add ADP-ribose to substrate protein via cleavage of NAD+ and release of nicotinamide. This poly (ADP-ribosyl)ation (“PARylation”) activity is activated by DNA strand breaks, which leads to addition of Par to PARP itself and other DNA repair enzymes. PARP is critical for the recruitment of DNA repair proteins to the damage sites.
Homologous recombination is a DNA repair process crucial for the accurate repair of DNA damage. BRCA1/2 genes, along with other Fanconi anemia pathway genes (e.g., RAD51D, NBN, ATM), are components of homologous recombination-mediated DNA repair. Mutations in the genes encoding homologous recombination factors play roles in the development of certain cancers. PARP inhibitors prevent the repair of DNA single-stranded breaks and promote the conversion of single-stranded breaks to double-stranded breaks, which creates synthetic lethality in cancer cells that lack proficient double-stranded break mechanisms such as homologous recombination.
There remains an unmet medical need for more effective therapies, e.g., combination therapies, for the treatment of cancers.
Combinations of (i) a ubiquitin-specific-processing protease 1 (USP1) inhibitor or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and (ii) a poly ADP-ribose polymerase (PARP) inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are provided herein. Also provided herein are methods of treating a subject with cancer using such a combination.
In one aspect, the present disclosure relates to a method of treating cancer in a subject who previously received treatment with a first poly ADP-ribose polymerase (PARP) inhibitor, the method comprising administering to the subject a ubiquitin-specific-processing protease (USP1) inhibitor and a second PARP inhibitor, wherein the first and the second PARP inhibitors are the same or different PARP inhibitors.
In one aspect, the subject did not previously receive treatment with a USP1 inhibitor.
In one aspect, the treatment with the first PARP inhibitor was interrupted or discontinued. In one aspect, the interruption is for at least one week, at least two weeks, at least three weeks, or at least four weeks. In one aspect, the interruption is for no more than four weeks.
In one aspect, the subject experienced unacceptable toxicity and/or unacceptable adverse reactions during treatment with the first PARP inhibitor.
In one aspect, the unacceptable toxicity or adverse reaction was a hematological toxicity such as thrombocytopenia, anemia, or neutropenia, pneumonitis, dyspnea, fever, cough, wheezing, a radiological abnormality, hypertension, myelodysplastic syndrome/acute myeloid leukemia (MDS/AML), nausea, and/or fatigue.
In one aspect, during treatment with the first PARP inhibitor, the dose of the first PARP inhibitor was reduced. In one aspect, the dose of the first PARP inhibitor was reduced to one quarter, one third, one half, two thirds, or three quarters of the dose prior to the reduction.
In one aspect, the first PARP inhibitor was olaparib and the dose prior to the reduction was 400 mg taken twice daily. In one aspect, the first PARP inhibitor was olaparib, and the dose after the reduction was 200 mg taken twice daily or 100 mg taken twice daily.
In one aspect, the first PARP inhibitor was niraparib and the dose prior to reduction was 300 mg taken once daily. In one aspect, the first PARP inhibitor was niraparib, and the dose after the reduction was 200 mg taken once daily or 100 mg taken once daily.
In one aspect, the first PARP inhibitor was talazoparib and the dose prior to reduction was 1 mg taken once daily. In one aspect, the first PARP inhibitor was talazoparib, and the dose after the reduction was 0.75 mg taken once daily, 0.5 mg taken once daily, or 0.25 mg taken once daily.
In one aspect, the first PARP inhibitor was rucaparib and the dose prior to reduction was 600 mg taken twice daily. In one aspect, the first PARP inhibitor was rucaparib, and the dose after the reduction was 500 mg taken twice daily, 400 mg taken twice daily, or 300 mg taken twice daily.
In one aspect, the first PARP inhibitor was olaparib, niraparib, talazoparib, or rucaparib. In one aspect, the second PARP inhibitor is olaparib, niraparib, talazoparib, or rucaparib. In one aspect, the first PARP inhibitor was olaparib and the second PARP inhibitor is olaparib. In one aspect, the first PARP inhibitor was niraparib and the second PARP inhibitor is niraparib. In one aspect, the first PARP inhibitor was talazoparib and the second PARP inhibitor is talazoparib. In one aspect, the first PARP inhibitor was rucaparib and the second PARP inhibitor is rucaparib.
In one aspect, the first PARP inhibitor and the second PARP inhibitor are the same PARP inhibitor. In one aspect, the first PARP inhibitor and the second PARP inhibitor are different PARP inhibitors.
In one aspect, the dose of the second PARP inhibitor is reduced compared to the dose of first PARP inhibitor.
In one aspect, the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect, the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect, the USP1 inhibitor and the second PARP inhibitor are well tolerated.
In one aspect, the USP1 inhibitor decreases the exposure of the subject to the second PARP inhibitor.
In one aspect, the USP1 inhibitor and the second PARP inhibitor inhibit rebounding and/or regrowth of the cancer.
In one aspect, the USP1 inhibitor and the second PARP inhibitor are administered sequentially. In one aspect, the USP1 inhibitor and the second PARP inhibitor are administered simultaneously.
In one aspect, the subject is human.
In one aspect, the cancer is selected from the group consisting of brain cancer, lung cancer, non-small cell lung cancer (NSCLC), colon cancer, bladder cancer, osteosarcoma, ovarian cancer, skin cancer, uterine cancer, peritoneal cancer, and endometrial cancer, and breast cancer. In one aspect, the cancer is breast cancer. In one aspect, the breast cancer is triple negative breast cancer (TNBC). In one aspect, the cancer is a BRCA1 mutant cancer, a BRCA2 mutant cancer, or a BRCA1 mutant and BRCA2 mutant cancer. In one aspect, the cancer is ovarian cancer. In one aspect, the ovarian cancer is a BRCA1 mutant cancer, a BRCA2 mutant cancer, or a p53 mutant cancer. In one aspect, the ovarian cancer is a BRCA1 mutant cancer and a p53 mutant cancer. In one aspect, the ovarian cancer is a BRCA1 and BRCA2 mutant cancer. In one aspect, the ovarian cancer is a BRCA2 mutant cancer. In one aspect, the cancer is selected from the group consisting of a hematological cancer and a lymphatic cancer.
In one aspect, the cancer comprises cells with elevated levels of RAD51. In one aspect, the elevated levels of RAD51 are elevated RAD51 protein levels. In one aspect, the elevated levels of RAD51 are elevated RAD51 protein foci levels. In one aspect, at least 10% of cells that are in the S/G2 phase of the cell cycle in a sample obtained from the cancer are RAD51-positive. In one aspect, the elevated levels of RAD51 are elevated RAD51 mRNA levels. In one aspect, the elevated levels of RAD51 have been detected prior to the administration. In one aspect, the method further comprises detecting RAD51 levels in a cancer sample obtained from the subject prior to the administration.
In one aspect, the cancer is selected from the group consisting of a DNA damage repair pathway deficient cancer, a homologous-recombination deficient cancer, a cancer comprising cancer cells with a mutation in a gene encoding p53, a cancer comprising cancer cells with a loss of function mutation in a gene encoding p53, and a cancer comprising cells with a mutation in the gene encoding ATM.
In one aspect, the cancer is a PARP inhibitor resistant or refractory cancer.
In one aspect, the present disclosure relates to a method of treating cancer in a subject comprising administering to the subject a USP1 inhibitor, wherein the cancer comprises cancer cells with elevated levels of RAD51. In one aspect, the elevated levels of RAD51 have been detected prior to the administration. In one aspect, the method further comprises detecting RAD51 levels in a cancer sample obtained from the subject. In one aspect, the method further comprises administering to the subject a PARP inhibitor in combination with the USP1 inhibitor. In one aspect, the PARP inhibitor is olaparib, niraparib, talazoparib, or rucaparib.
In one aspect, the present disclosure relates to a method of selecting a subject with cancer for treatment with a USP1 inhibitor, comprising detecting whether the cancer comprises cells with elevated levels of RAD51, wherein if the cancer comprises cells with elevated levels of RAD51, the subject is selected for treatment with a USP1 inhibitor.
In one aspect, the present disclosure relates to an in vitro method for identifying a subject with cancer to be responsive to the treatment with a USP1 inhibitor, comprising detecting RAD51 levels in a cancer sample obtained from the subject, wherein elevated levels of RAD51 in the cancer sample are indicative for the patient to be responsive to the treatment with a USP1 inhibitor.
In one aspect, the present disclosure relates to an in vitro use of at least one agent capable of specifically detecting RAD51, for identifying a subject with cancer to be responsive to the treatment with a USP1 inhibitor.
In one aspect of any method or use provided herein, the treatment with a USP1 inhibitor further comprises treatment with a PARP inhibitor in combination with the USP1 inhibitor. In one aspect, the PARP inhibitor is olaparib, niraparib, talazoparib, or rucaparib. In one aspect, the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect of any method or use provided herein, the subject is human. In one aspect, the cancer is selected from the group consisting of brain cancer, lung cancer, non-small cell lung cancer (NSCLC), colon cancer, bladder cancer, osteosarcoma, ovarian cancer, skin cancer, uterine cancer, peritoneal cancer, and endometrial cancer, and breast cancer. In one aspect, the cancer is breast cancer. In one aspect, the breast cancer is triple negative breast cancer (TNBC). In one aspect, the cancer is a BRCA1 mutant cancer, a BRCA2 mutant cancer, or a BRCA1 mutant and BRCA2 mutant cancer. In one aspect, the cancer is ovarian cancer. In one aspect, the ovarian cancer is a BRCA1 mutant cancer, a BRCA2 mutant cancer, or a p53 mutant cancer. In one aspect, the ovarian cancer is a BRCA1 mutant cancer and a p53 mutant cancer. In one aspect, the ovarian cancer is a BRCA1 and BRCA2 mutant cancer. In one aspect, the ovarian cancer is a BRCA2 mutant cancer.
In one aspect, the present disclosure relates to a method of delaying, reducing, or preventing rebounding of a tumor in a subject comprising administering to the subject (i) a ubiquitin-specific-processing protease 1 (USP1) inhibitor and (ii) a poly ADP-ribose polymerase (PARP) inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect, the present disclosure relates to a method of delaying, reducing, or preventing rebounding of a tumor in a subject comprising administering to the subject (i) a ubiquitin-specific-processing protease 1 (USP1) inhibitor and (ii) a poly ADP-ribose polymerase (PARP) inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof
In one aspect, the present disclosure relates to a combination composition comprising (i) a ubiquitin-specific-processing protease 1 (USP1) inhibitor and (ii) a poly ADP-ribose polymerase (PARP) inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect, the present disclosure relates to a combination composition comprising (i) a ubiquitin-specific-processing protease 1 (USP1) inhibitor and (ii) a poly ADP-ribose polymerase (PARP) inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect, the present disclosure relates to a combination composition comprising (i) a ubiquitin-specific-processing protease 1 (USP1) inhibitor and (ii) a poly ADP-ribose polymerase (PARP) inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In some aspects, the PARP inhibitor is selected from the group consisting of olaparib (Lynparza®), rucaparib (Rubraca®), niraparib (Zejula®), and talazoparib (Talzenna®), and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect, the PARP inhibitor is niraparib or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In another aspect, the PARP inhibitor is olaparib or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In one aspect, the USP1 inhibitor is the compound of Formula I, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In another aspect, the USP1 inhibitor is the compound of Formula II, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In another aspect, the USP1 inhibitor is the compound of Formula III, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In one aspect, the present disclosure relates to the use of the combination composition for the manufacture of a medicament for treatment of cancer.
In another aspect, the present disclosure relates to a pharmaceutical combination composition comprising the combination composition and a pharmaceutically acceptable carrier.
In one aspect, the pharmaceutical composition is for use in the treatment of cancer.
In one aspect, the present disclosure relates to a kit comprising the combination composition or the pharmaceutical combination composition, and instructions for administering the combination to a subject having cancer.
In another aspect, the present disclosure relates to a method of treating cancer in a subject comprising administering to the subject (i) USP1 inhibitor and (ii) PARP inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof,
wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In another aspect, the present disclosure relates to a method of treating cancer in a subject comprising administering to the subject (i) USP1 inhibitor and (ii) PARP inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof,
wherein the USP1 inhibitor is a compound selected from the group consisting of
and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In some aspects of the method, the PARP inhibitor is selected from the group consisting of niraparib, olaparib and pharmaceutically acceptable salts, hydrates, solvates, amorphous solids, or polymorphs thereof.
In one aspect of the method, the PARP inhibitor is niraparib or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In another aspect of the method, the PARP inhibitor is olaparib or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In one aspect of the method, the USP1 inhibitor is the compound of Formula I, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In another aspect of the method, the USP1 inhibitor is the compound of Formula II, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In another aspect of the method, the USP1 inhibitor is the compound of Formula III, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In one aspect of the present disclosure, the administration of the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, provides a synergistic effect.
In one aspect of the present disclosure, the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are administered in a therapeutically effective amount sufficient to produce one or more therapeutic effects selected from the group consisting of (i) a reduction in tumor size, (ii) an increase in cancer tumor regression rate, (iii) a reduction or inhibition of cancer tumor growth, and (iv) a reduction of the toxicity effects of a PARP inhibitor administered as a monotherapy. In one aspect of the present disclosure, the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are administered in a therapeutically effective amount sufficient to produce one or more therapeutic effects selected from the group consisting of (i) a reduction in tumor size, (ii) an increase in cancer tumor regression rate, and (iii) a reduction or inhibition of cancer tumor growth. In one aspect of the present disclosure, the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are administered in an amount sufficient to reduce the toxicity effects of a PARP inhibitor administered as a monotherapy.
In one aspect, the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are administered in a therapeutically effective amount sufficient to delay, reduce, or prevent rebounding (rapid re-growth) of a tumor.
In one aspect, the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are administered sequentially.
In another aspect, the USP1 inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and the PARP inhibitor, or said pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, are administered simultaneously.
In one aspect of the present disclosure, the combination is administered to a mammal. In another aspect, the mammal is a human.
In some aspects, the cancer is selected from the group consisting of a hematological cancer, a lymphatic cancer, a solid tumor, a DNA damage repair pathway deficient cancer and a homologous-recombination deficient cancer.
In some aspects, the cancer is selected from the group consisting of brain cancer, lung cancer, non-small cell lung cancer (NSCLC), colon cancer, bladder cancer, osteosarcoma, ovarian cancer, skin cancer, uterine cancer, peritoneal cancer, and endometrial cancer, and breast cancer.
In some aspects, the cancer is non-small cell lung cancer (NSCLC).
In some aspects, the cancer is colon cancer.
In some aspects, the cancer is bladder cancer.
In some aspects, the cancer is ovarian cancer or breast cancer.
In some aspects, the cancer is ovarian cancer.
In some aspects, the cancer is breast cancer.
In some aspects, the cancer is triple negative breast cancer.
In some aspects, the cancer is selected from the group consisting of bone cancer, including osteosarcoma and chondrosarcoma; brain cancer, including glioma, glioblastoma, astrocytoma, medulloblastoma, and meningioma; soft tissue cancer, including rhabdoid and sarcoma; kidney cancer; bladder cancer; skin cancer, including melanoma; and lung cancer, including non-small cell lung cancer; colon cancer, uterine cancer; nervous system cancer; head and neck cancer; pancreatic cancer; and cervical cancer.
In some aspects, the cancer is a DNA damage repair pathway deficient cancer.
In some aspects, the cancer is a BRCA1 mutant cancer. In some aspects, the BRCA1 mutation is a germline mutation. In some aspects, the BRCA1 mutation is a somatic mutation. In some aspects, the BRCA1 mutation leads to BRCA1 deficiency.
In some aspects, the cancer is a BRCA2 mutant cancer. In some aspects, the BRCA2 mutation is a germline mutation. In some aspects, the BRCA2 mutation is a somatic mutation. In some aspects, the BRCA2 mutation leads to BRCA2 deficiency.
In some aspects, the cancer is a BRCA1 mutant cancer and a BRCA2 mutant cancer.
In some aspects, the cancer is a BRCA1 deficient cancer.
In some aspects, the cancer is a BRCA2 deficient cancer.
In some aspects, the cancer is a BRCA1 deficient cancer and a BRCA2 deficient cancer.
In some aspects, the cancer is a PARP inhibitor refractory or resistant cancer. In some aspects, the cancer is a PARP inhibitor resistant or refractory BRCA1, BRCA2, or BRCA1 and BRCA2 mutant cancer. In some aspects, the cancer is a PARP inhibitor resistant or refractory BRCA1, BRCA2, or BRCA1 and BRCA2-deficient cancer.
In some aspects, the cancer has a mutation in the gene encoding ataxia telangiectasia mutated (ATM) protein kinase. In some aspects, the ATM mutation is a germline mutation. In some aspects, the ATM mutation is a somatic mutation. In some aspects, the cancer is an ATM-deficient cancer.
In some aspects, the cancer comprises cancer cells with a mutation in a gene encoding p53. In some aspects, the mutation in a gene encoding p53 is a germline mutation. In some aspects, the mutation in a gene encoding p53 is a somatic mutation. In some aspects, the cancer comprises cancer cells with a loss of function mutation in a gene encoding p53.
In some aspects, the cancer has a mutation in the gene encoding at least two of p53, BRCA1, BRCA2, and ATM.
In some aspects, the cancer comprises cells with elevated levels of RAD51. In some aspects, the elevated levels of RAD51 are elevated RAD51 protein levels. In some aspects, the elevated levels of RAD51 are elevated RAD51 protein foci levels. In some aspects, at least 10% of cells that are in the S/G2 phase of the cell cycle in a sample obtained from the cancer are RAD51-positive. In some aspects, the elevated levels of RAD51 are elevated RAD51 mRNA levels. In some aspects, the elevated levels of RAD51 have been detected prior to the administration or the treatment. In some aspects, a method or use provided herein further comprises detecting RAD51 levels in a cancer sample obtained from the subject prior to the administration or the treatment.
In another aspect, the present disclosure relates to a method of treating a USP1 protein mediated disorder and/or a PARP protein mediated disorder comprising administering to a subject in need thereof a USP1 inhibitor of Formula I or II, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and a PARP inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, in an effective amount to treat the USP1 protein mediated disorder and/or the PARP protein mediated disorder. In another aspect, the present disclosure relates to a method of treating a USP1 protein mediated disorder and/or a PARP protein mediated disorder comprising administering to a subject in need thereof a USP1 inhibitor of Formula I, Formula II, or Formula III, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and a PARP inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, in an effective amount to treat the USP1 protein mediated disorder and/or the PARP protein mediated disorder.
In another aspect, the present disclosure relates to a method of inhibiting a USP1 protein and/or a PARP protein comprising contacting a USP1 protein and/or a PARP protein with a USP1 inhibitor of Formula I or II, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and a PARP inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof. In another aspect, the present disclosure relates to a method of inhibiting a USP1 protein and/or a PARP protein comprising contacting a USP1 protein and/or a PARP protein with a USP1 inhibitor of Formula I, Formula II, or Formula III, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof, and a PARP inhibitor, or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
In some aspects, the contacting occurs in vitro.
In some aspects, the contacting occurs in vivo.
Additional aspects and advantages of the disclosure will be set forth, in part, in the description that follows, and will flow from the description, or can be learned by practice of the disclosure. The aspects and advantages of the disclosure will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims.
It is to be understood that both the foregoing summary and the following detailed description are exemplary and explanatory only, and are not restrictive of the invention as claimed.
One aspect of the present disclosure is based on the use of a combination of a ubiquitin-specific-processing protease 1 (USP1) protein inhibitor and a poly ADP-ribose polymerase (PARP) inhibitor. The combinations are useful for inhibiting a USP1 protein and/or a PARP protein and for treating diseases, disorders, or conditions, e.g., cancer, that are responsive to inhibition of a USP1 protein and/or a PARP protein.
In some aspects, the combination of a USP1 inhibitor and a PARP inhibitor provide a synergistic effect.
In some aspects, the USP1 inhibitor and the PARP inhibitor are in therapeutically effective amounts sufficient to produce a therapeutic effect comprising: (i) a reduction in size of a tumor, (ii) an increase in cancer tumor regression rate, (iii) a reduction or inhibition of cancer tumor growth, and/or (iv) a reduction of the toxicity effects of a PARP inhibitor administered as a monotherapy. In some aspects, the USP1 inhibitor and the PARP inhibitor can delay, reduce, or prevent rebounding (rapid re-growth) of a tumor.
The tolerability (lack of toxicity) of combinations provided herein is particular surprising given that other combinations with the PARP inhibitor Olaparib have not been well-tolerated. See, e.g., Samol, J., et al., Invest. New Drugs, 30:1493-500 (2012) (“Further development of olaparib and topotecan in combination was not explored due to dose-limiting hematological AEs and the resulting sub-therapeutic MTD.”).
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. In case of conflict, the present application including the definitions will control. Unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular. All publications, patents and other references mentioned herein are incorporated by reference in their entireties for all purposes as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference.
Although methods and materials similar or equivalent to those described herein can be used in practice or testing of the present disclosure, suitable methods and materials are described below. The materials, methods and examples are illustrative only and are not intended to be limiting. Other features and advantages of the disclosure will be apparent from the detailed description and from the claims.
In order to further define this disclosure, the following terms and definitions are provided.
It is understood that embodiments described herein include “consisting” and/or “consisting essentially of” embodiments. As used herein, the singular form “a,” “an,” and “the” includes plural references unless indicated otherwise. Use of the term “or” herein is not meant to imply that alternatives are mutually exclusive.
In this application, the use of “or” means “and/or” unless expressly stated or understood by one skilled in the art. In the context of a multiple dependent claim, the use of “or” refers back to more than one preceding independent or dependent claim.
The term “and/or” where used herein is to be taken as specific disclosure of each of the two specified features or components with or without the other. Thus, the term “and/or” as used in a phrase such as “A and/or B” herein is intended to include “A and B,” “A or B,” “A” (alone), and “B” (alone). Likewise, the term “and/or” as used in a phrase such as “A, B, and/or C” is intended to encompass each of the following aspects: A, B, and C; A, B, or C; A or C; A or B; B or C; A and C; A and B; B and C; A (alone); B (alone); and C (alone).
The term “about,” as used herein, includes the recited number±10%. Thus, “about 10” means 9 to 11. As is understood by one skilled in the art, reference to “about” a value or parameter herein includes (and describes) instances that are directed to that value or parameter per se. For example, description referring to “about X” includes description of “X.”
The present disclosure encompasses the preparation and use of salts of the USP1 inhibitors and PARP inhibitors, including non-toxic pharmaceutically acceptable salts. Examples of pharmaceutically acceptable addition salts include inorganic and organic acid addition salts and basic salts. Pharmaceutically acceptable salts include, but are not limited to, metal salts such as sodium salt, potassium salt, cesium salt and the like; alkaline earth metals such as calcium salt, magnesium salt and the like; organic amine salts such as triethylamine salt, pyridine salt, picoline salt, ethanolamine salt, triethanolamine salt, dicyclohexylamine salt, N,N′-dibenzylethylenediamine salt and the like; inorganic acid salts such as hydrochloride, hydrobromide, phosphate, sulphate and the like; organic acid salts such as citrate, lactate, tartrate, maleate, fumarate, mandelate, acetate, dichloroacetate, trifluoroacetate, oxalate, formate and the like; sulfonates such as methanesulfonate, benzenesulfonate, p-toluenesulfonate and the like; and amino acid salts such as arginate, asparginate, glutamate and the like. The term “pharmaceutically acceptable salt” as used herein, refers to any salt, e.g., obtained by reaction with an acid or a base, of a USP1 inhibitor or PARP inhibitor of the disclosure that is physiologically tolerated in the target patient (e.g., a mammal, e.g., a human).
Acid addition salts can be formed by mixing a solution of the particular USP1 inhibitor or PARP inhibitor with a solution of a pharmaceutically acceptable non-toxic acid such as hydrochloric acid, fumaric acid, maleic acid, succinic acid, acetic acid, citric acid, tartaric acid, carbonic acid, phosphoric acid, oxalic acid, dichloroacetic acid, or the like. Basic salts can be formed by mixing a solution of the USP1 inhibitor or PARP inhibitor of the present disclosure with a solution of a pharmaceutically acceptable non-toxic base such as sodium hydroxide, potassium hydroxide, choline hydroxide, sodium carbonate and the like.
In some aspects of the disclosure, a pharmaceutically acceptable salt is formed between a compound of Formula I or Formula II and a pharmaceutically acceptable acid. In some aspects of the disclosure, a pharmaceutically acceptable salt is formed between a compound of Formula I, Formula II, or Formula III and a pharmaceutically acceptable acid. In some aspects, the pharmaceutically acceptable acid is selected from the group consisting of 1-hydroxy-2-naphthoic acid, 4-aminosalicylic acid, ascorbic acid, adipic acid, L-aspartic acid, benzene sulfonic acid, benzoic acid, trans-cinnamic acid, citric acid, ethanedisulfonic acid, fumaric acid, galactaric acid, gallic acid, gentisic acid, gluconic acid, D-glucuronic acid, glutamic acid, glutaric acid, glycolic acid, hexanoic acid, hippuric acid, hydrobromic acid, hydrochloric acid, lactic acid, maleic acid, L-malic acid, malonic acid, R-mandelic acid, methanesulfonic acid, mucic acid, naphthalene sulfonic acid, nicotinic acid, oxalic acid, palmitic acid, p-toluene sulfonic acid, phosphoric acid, propionic acid, saccharin, salicylic acid, stearic acid, succinic acid, sulfuric acid, L-tartaric acid, vanillic acid, and vanillin. In some aspects, the pharmaceutically acceptable acid is selected from the group consisting of benzoic acid, gallic acid, gentisic acid and salicylic acid.
The present disclosure encompasses the preparation and use of solvates of the USP1 inhibitor and/or PARP inhibitor. Solvates typically do not significantly alter the physiological activity or toxicity of the compounds, and as such may function as pharmacological equivalents. The term “solvate” as used herein is a combination, physical association and/or solvation of a USP1 inhibitor or PARP inhibitor of the present disclosure with a solvent molecule such as, e.g. a disolvate, monosolvate or hemisolvate, where the ratio of solvent molecule to compound of the present disclosure is about 2:1, about 1:1 or about 1:2, respectively. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances, the solvate can be isolated, such as when one or more solvent molecules are incorporated into the crystal lattice of a crystalline solid. Thus, “solvate” encompasses both solution-phase and isolatable solvates. USP1 inhibitors or PARP inhibitors of the disclosure can be present as solvated forms with a pharmaceutically acceptable solvent, such as water, methanol, ethanol, and the like, and it is intended that the disclosure includes both solvated and unsolvated forms of the USP1 inhibitor and/or PARP inhibitor of the disclosure. One type of solvate is a hydrate. A “hydrate” relates to a particular subgroup of solvates where the solvent molecule is water. Solvates typically can function as pharmacological equivalents. Preparation of solvates is known in the art. See, for example, M. Caira et al, J. Pharmaceut. Sci., 93(3):601-611 (2004), which describes the preparation of solvates of fluconazole with ethyl acetate and with water. Similar preparation of solvates, hemisolvates, hydrates, and the like are described by E.C. van Tonder et al., AAPS Pharm. Sci. Tech., 5(1):Article 12 (2004), and A. L. Bingham et al., Chem. Commun. 603-604 (2001). Atypical, non-limiting, process of preparing a solvate would involve dissolving a USP1 inhibitor or PARP inhibitor of the disclosure in a desired solvent (organic, water, or a mixture thereof) at temperatures above 20° C. to about 25° C., then cooling the solution at a rate sufficient to form crystals, and isolating the crystals by known methods, e.g., filtration. Analytical techniques such as infrared spectroscopy can be used to confirm the presence of the solvent in a crystal of the solvate.
In some aspects of the disclosure, the USP1 inhibitor and/or PARP inhibitor is deuterated. In some aspects, the USP1 inhibitor and/or PARP inhibitor are partially or completely deuterated, i.e., one or more hydrogen atoms are replaced with deuterium atoms.
As used herein, “treatment” is an approach for obtaining beneficial or desired clinical results. “Treatment” as used herein, covers any administration or application of a therapeutic for disease in a mammal, including a human. For purposes of this disclosure, beneficial or desired clinical results include, but are not limited to, any one or more of: alleviation of one or more symptoms, diminishment of extent of disease, preventing or delaying spread (for example, metastasis) of disease, preventing or delaying recurrence of disease, delay or slowing of disease progression, amelioration of the disease state, inhibiting the disease or progression of the disease, inhibiting or slowing the disease or its progression, arresting its development, and remission (whether partial or total). Also encompassed by “treatment” is a reduction of pathological consequence of a proliferative disease. The methods provided herein contemplate any one or more of these aspects of treatment. In-line with the above, the term treatment does not require one-hundred percent removal of all aspects of the disorder.
In the context of cancer, the term “treating” includes, but is not limited to, inhibiting growth of cancer cells, inhibiting replication of cancer cells, lessening of overall tumor burden, and delaying, halting, or slowing tumor growth, progression, or metastasis.
As used herein, “delaying” means to defer, hinder, slow, retard, stabilize, suppress and/or postpone development or progression of the disease (such as cancer). This delay can be of varying lengths of time, depending on the history of the disease and/or individual being treated.
A “therapeutically effective amount” of a substance can vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of the substance to elicit a desired response in the individual. A therapeutically effective amount is also one in which any toxic or detrimental effects of the substance are outweighed by the therapeutically beneficial effects. A therapeutically effective amount can be delivered in one or more administrations. A therapeutically effective amount refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic effect.
The terms “combination,” “therapeutic combination,” “combination composition,” “combination therapy” or “pharmaceutical combination”, as used herein, can include a fixed combination in one dosage unit form, separate dosage units or a kit of parts or instructions for the combined administration where the USP1 inhibitor and the PARP inhibitor can be administered independently at the same time or separately within time intervals. A combined pharmaceutical composition can be adapted for simultaneous, separate, or sequential administration.
The combination therapy can provide “synergy” and prove “synergistic,” i.e., the effect achieved when the active ingredients used together is greater than the sum of the effects that results from using the compounds separately. A synergistic effect can include a significantly reduced effective dose for the combination of the two active ingredients as compared to the effective dose of each active ingredient when administered separately. A synergistic effect can also include a reduction in toxicity for the combination of the two active ingredients as compared to the toxicity of each active ingredient when administered separately. A synergistic effect can also be an effect that cannot be achieved by administration of any of the active ingredients as single agents. The synergistic effect can include, but is not limited to, an effect of treating cancer by reducing tumor size, inhibiting tumor growth, or increasing survival of the subject. The synergistic effect can also include reducing cancer cell viability, inducing cancer cell death, and inhibiting or delaying cancer cell growth. A synergistic effect can be attained, for example, when the active ingredients are: (1) co-formulated and administered or delivered simultaneously in a combined, unit dosage formulation; (2) delivered serially, by alternation, or in parallel as separate formulations; or (3) by some other regimen. When delivered in alternation therapy, a synergistic effect can be attained when the compounds are administered or delivered sequentially.
A determination of a synergistic interaction between a USP1 inhibitor and a PARP inhibitor can be based on the results obtained from the assays described herein. For example, combination effects can be evaluated using the Bliss independence model. Bliss scores quantify degree of potentiation from single agents, and a Bliss score >0 suggests greater than simple additivity. In some aspects, a Bliss score greater than 10 indicates strong synergy. In other aspects, a score of 6 or greater indicates synergy. In some aspects, the Bliss score is about 6, about 7, about 8, about 9, about 10, about 11, about 12, about 13, about 14, about 15, about 16, about 17, about 18, about 19, about 20 or about 25.
As used herein, a “homologous recombination deficiency score” or “HRD score” means an algorithmic assessment of three measures of tumor genomic instability, i.e., loss of heterozygosity, telomeric allelic imbalance and large-scale state transitions.
The terms “administer,” “administering,” “administration,” and the like refer to methods that can be used to enable delivery of the therapeutic agent to the desired site of biological action. Administration techniques that can be employed with the agents and methods described herein are found in e.g., Goodman and Gilman, The Pharmacological Basis of Therapeutics, current ed.; Pergamon; and Remington's, Pharmaceutical Sciences (current edition), Mack Publishing Co., Easton, Pa. Administration of two or more therapeutic agents includes simultaneous (concurrent) and consecutive administration in any order.
The terms “pharmaceutical formulation” and “pharmaceutical composition” refer to a preparation which is in such form as to permit the biological activity of the active ingredient(s) to be effective, and which contains no additional components which are unacceptably toxic to a subject to which the formulation would be administered. Such formulations may be sterile.
The term “pharmaceutically acceptable” as used herein refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.
A “pharmaceutically acceptable carrier” refers to a non-toxic solid, semisolid, or liquid filler, diluent, encapsulating material, formulation auxiliary, or carrier conventional in the art for use with a therapeutic agent that together comprise a “pharmaceutical composition” for administration to a subject. A pharmaceutically acceptable carrier is non-toxic to recipients at the dosages and concentrations employed and is compatible with other ingredients of the formulation. The pharmaceutically acceptable carrier is appropriate for the formulation employed.
A “sterile” formulation is aseptic or essentially free from living microorganisms and their spores.
The term “container” means any receptacle and closure therefore suitable for storing, shipping, dispensing, and/or handling a pharmaceutical product.
The term “insert” or “package insert” means information accompanying a pharmaceutical product that provides a description of how to administer the product, along with the safety and efficacy data required to allow the physician, pharmacist, and patient to make an informed decision regarding use of the product. The package insert generally is regarded as the “label” for a pharmaceutical product.
The term “disease” or “condition” or “disorder” as used herein refers to a condition where treatment is needed and/or desired and denotes disturbances and/or anomalies that as a rule are regarded as being pathological conditions or functions, and that can manifest themselves in the form of particular signs, symptoms, and/or malfunctions. As demonstrated below, combinations of the USP1 inhibitors and PARP inhibitors of the present disclosure can be used in treating diseases and conditions, such as proliferative diseases, wherein inhibition of USP1 and/or PARP proteins provides a benefit.
The terms “polypeptide” and “protein” are used interchangeably to refer to a polymer of amino acid residues and are not limited to a minimum length. Such polymers of amino acid residues may contain natural or non-natural amino acid residues, and include, but are not limited to, peptides, oligopeptides, dimers, trimers, and multimers of amino acid residues. Both full-length proteins and fragments thereof are encompassed by the definition. The terms also include post-expression modifications of the polypeptide, for example, glycosylation, sialylation, acetylation, phosphorylation, and the like. Furthermore, for purposes of the present disclosure, a “polypeptide” refers to a protein which includes modifications, such as deletions, additions, and substitutions (generally conservative in nature), to the native sequence, as long as the protein maintains the desired activity. These modifications may be deliberate, as through site-directed mutagenesis, or may be accidental, such as through mutations of hosts which produce the proteins or errors due to PCR amplification.
“USP1” and “ubiquitin-specific-processing protease 1” as used herein refer to any native polypeptide or USP1-encoding polynucleotide. The term “USP1” encompasses “full-length,” unprocessed USP1 polypeptide as well as any forms of USP1 that result from processing within the cell (e.g., removal of the signal peptide). The term also encompasses naturally occurring variants of USP1, e.g., those encoded by splice variants and allelic variants. The USP1 polypeptides described herein can be isolated from a variety of sources, such as from human tissue types or from another source, or prepared by recombinant or synthetic methods. Human USP1 sequences are known and include, for example, the sequences publicly available as UniProt No. 094782 (including isoforms). As used herein, the term “human USP1 protein” refers to USP1 protein comprising the amino acid sequence as set forth in SE ID NO:1:
USP1 is a deubiquitinating enzyme that acts as part of a complex with UAF1. USP1's “deubiquitinase activity” includes its ability to deubiquitinate as part of the USP1-UAF1 complex.
“PARP” or “PARP protein” as used herein refers to one or more of the Poly (ADP-ribose) polymerase family of enzymes. The family includes enzymes that have the ability to catalyze the transfer of ADP-ribose to target proteins (poly ADP-ribosylation). There are at least 18 members of the PARP family that are encoded by different genes, and share homology in a conserved catalytic domain, including PARP-1, PARP-2 and PARP-3.
The term “specifically binds” is well understood in the art, and methods to determine such specific binding are also well known in the art. A molecule is said to exhibit “specific binding” or “preferential binding” if it reacts or associates more frequently, more rapidly, with greater duration and/or with greater affinity with a particular protein or domain of a protein than it does with alternative proteins or domains. It should be understood that a molecule that specifically or preferentially binds to a first protein or domain may or may not specifically or preferentially bind to a second protein or domain. As such, “specific binding” or “preferential binding” does not necessarily require (although it can include) exclusive binding. Generally, but not necessarily, reference to binding means preferential binding. For example, a USP1 inhibitor that specifically binds to USP1, UAF1, and/or the USP1-UAF1 complex may not bind to other deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) or may bind to other deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) with a reduced affinity as compared to binding to USP1.
The terms “reduction” or “reduce” or “inhibition” or “inhibit” refer to a decrease or cessation of any phenotypic characteristic or to the decrease or cessation in the incidence, degree, or likelihood of that characteristic. To “reduce” or “inhibit” is to decrease, reduce or arrest an activity, function, and/or amount as compared to a reference. In some embodiments, by “reduce” or “inhibit” is meant the ability to cause an overall decrease of 20% or greater. In some embodiments, by “reduce” or “inhibit” is meant the ability to cause an overall decrease of 50% or greater. In some embodiments, by “reduce” or “inhibit” is meant the ability to cause an overall decrease of 75%, 85%, 90%, 95%, or greater. In some embodiments, the amount noted above is inhibited or decreased over a period of time, relative to a control over the same period of time.
In some aspects, inhibiting USP1 proteins is the inhibition of one or more activities or functions of USP1 proteins. It should be appreciated that the activity or function of the one or more USP1 proteins may be inhibited in vitro or in vivo. Non-limiting examples of activities and functions of USP1 include deubiquitinase activity and formation of a complex with UAF1 and are described herein. Exemplary levels of inhibition of the activity of one or more USP1 proteins include at least 10% inhibition, at least 20% inhibition, at least 30% inhibition, at least 40% inhibition, at least 50% inhibition, at least 60% inhibition, at least 70% inhibition, at least 80% inhibition, at least 90% inhibition, and up to 100% inhibition.
In some aspects, inhibiting PARP proteins is the inhibition of one or more activities or functions of PARP proteins. It should be appreciated that the activity or function of the one or more PARP proteins may be inhibited in vitro or in vivo. Non-limiting examples of activities and functions of PARP are described herein. Exemplary levels of inhibition of the activity of one or more PARP proteins include at least 10% inhibition, at least 20% inhibition, at least 30% inhibition, at least 40% inhibition, at least 50% inhibition, at least 60% inhibition, at least 70% inhibition, at least 80% inhibition, at least 90% inhibition, and up to 100% inhibition.
The terms “individual” or “subject” are used interchangeably herein to refer to an animal, for example, a mammal, such as a human. In some instances, methods of treating mammals, including, but not limited to, humans, rodents, simians, felines, canines, equines, bovines, porcines, ovines, caprines, mammalian laboratory animals, mammalian farm animals, mammalian sport animals, and mammalian pets, are provided. In some examples, an “individual” or “subject” refers to an individual or subject in need of treatment for a disease or disorder. In some instances, the subject to receive the treatment can be a patient, designating the fact that the subject has been identified as having a disorder of relevance to the treatment, or being at particular risk of contracting the disorder.
As used herein, the terms “cancer” and “tumor” refer to or describe the physiological condition in mammals in which a population of cells are characterized by unregulated cell growth. The terms encompass solid and hematological/lymphatic cancers. Examples of cancer include but are not limited to, DNA damage repair pathway deficient cancers. Additional examples of cancer include, but are not limited to, ovarian cancer, breast cancer (including triple negative breast cancer), non-small cell lung cancer (NSCLC), and osteosarcoma. The cancer can be BRCA1 or BRCA2 wild type. The cancer can also be BRCA1 or BRCA2 mutant. The cancer can further be a PARP inhibitor resistant or refractory cancer, or a PARP inhibitor resistant or refractory BRCA1 or BRCA2-mutant cancer.
As used herein, the term “loss of function” mutation refers to a mutation that results in the absence of a gene, decreased expression of a gene, or the production of a gene product (e.g. protein) having decreased activity or no activity. Loss of function mutations include for example, missense mutations, nucleotide insertions, nucleotide deletions, and gene deletions. Loss of function mutations also include dominant negative mutations. Thus, cancer cells with a loss of function mutation in a gene encoding p53 include cancer cells that contain missense mutations in a gene encoding p53 as well as cancer cells that lack a gene encoding p53.
In some aspects, the ubiquitin-specific-processing protease 1 (USP1) inhibitor of the disclosure comprises a compound of
or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
The chemical name for the USP1 inhibitor of Formula I is 6-(4-cyclopropyl-6-methoxypyrimidin-5-yl)-1-(4-(1-isopropyl-4-(trifluoromethyl)-1H-imidazol-2-yl)benzyl)-1H-pyrazolo[3,4-d]pyrimidine, as described in U.S. application Ser. No. 16/721,079.
The chemical name for the USP1 inhibitor of Formula II is 6-(4-cyclopropyl-6-methoxypyrimidin-5-yl)-1-(4-(1-methyl-4-(trifluoromethyl)-1H-imidazol-2-yl)benzyl)-1H-pyrazolo[3,4-d]pyrimidine, as described in U.S. application Ser. No. 16/721,079.
U.S. application Ser. No. 16/721,079 is herein incorporated by reference in its entirely.
In some aspects, the ubiquitin-specific-processing protease 1 (USP1) inhibitor of the disclosure comprises a compound of
or a pharmaceutically acceptable salt, hydrate, solvate, amorphous solid, or polymorph thereof.
The chemical name for the USP1 inhibitor of Formula III is 6-(4-cyclopropyl-6-methoxypyrimidin-5-yl)-1-(4-(5-methyl-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzyl)-1H-pyrazolo[3,4-d]pyrimidine, as described in U.S. application Ser. No. 16/721,079. U.S. application Ser. No. 16/721,079 is herein incorporated by reference in its entirely.
In various aspects, the USP1 inhibitors reduce the level of USP1 protein and/or inhibit or reduce at least one biological activity of USP1 protein.
In some aspects, the USP1 inhibitors specifically bind to USP1 protein. In some aspects, the USP1 inhibitors specifically bind to USP1 protein in a USP1-UAF1 complex. In some aspects, the USP1 inhibitors specifically bind to USP1 mRNA. In some aspects, the USP1 inhibitors specifically bind to USP1 protein (alone or in a USP1-UAF1 complex) or USP1 mRNA. In some aspects, the USP1 inhibitors specifically bind to UAF1 (alone or in a USP1-UAF1 complex) and inhibit or reduces formation or activity of the USP1-UAF1 complex.
In some aspects, the USP1 inhibitors decrease the formation of the USP1-UAF1 complex. In some aspects, the USP1 inhibitors decrease the activity of the USP1-UAF1 complex. In some aspects, the USP1 inhibitors decrease the deubiquitinase activity of USP1. In some aspects, the USP1 inhibitors increase mono-ubiquitinated PCNA. In some aspects, the USP1 inhibitors increase mono-ubiquitinated FANCD2. In some aspects, the USP1 inhibitors increase mono-ubiquitinated FANCI.
In some aspects, the USP1 inhibitors do not bind to other deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) or bind deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) with at least 5-fold, at least 10-fold, at least 20-fold, or at least 100-fold reduced affinity compared to the affinity for USP1 (i.e., the KD of the USP1 inhibitor for other deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) is at least 5-fold, at least 10-fold, at least 20-fold, or at least 100-fold higher than the KD for USP1).
In some aspects, the USP1 inhibitors inhibit USP1 deubiquitinase activity with an IC50 of less than about 50 nM, between about 50 nM and about 200 nM, between about 200 nM and about 2 pM, or greater than 2 pM, e.g., as measured using the assay disclosed in U.S. Patent Application Publication No. 2017/0145012 or IC50 of 50 nM to 1000 nM, e.g., as measured using the assay disclosed in Liang et al., Nat Chem Biol 10: 289-304 (2014). In some aspects, the USP1 inhibitors inhibit USP1 deubiquitinase activity with an IC50 as measured using the assay disclosed in Chen, et al., Chem Biol., 18(11):1390-1400 (2011). In some aspects, the USP1 inhibitors do not inhibit the activity of other deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) or inhibit the activity of other deubiquitinases, other USP proteins, or other UAF1 complexes (e.g., USP46-UAF1) with at least 5-fold, at least 10-fold, at least 20-fold, or at least 100-fold higher IC50 compared to the IC50 for inhibition of USP1 deubiquitinase activity.
In some aspects, the USP1 inhibitors of the present disclosure bind to a USP1 protein with an affinity in the range of 1 pM to 100 μM, or 1 pM to 1 μM, or 1 pM to 500 nM, or 1 pM to 100 nM. In some aspects, the USP1 inhibitors of the present disclosure bind to a USP1 protein with an affinity of about 1 pM to about 100 μM, about 1 nM to about 100 μM, about 1 μM to about 100 μM, about 1 μM to about 50 μM, about 1 μM to about 40 μM, about 1 μM to about 30 μM, about 1 μM to about 20 μM, or about 1 μM to about 10 μM, about 1 μM, about 5 μM, about 10 μM, about 15 μM, about 20 μM, about 25 μM, about 30 μM, about 35 μM, about 40 μM, about 45 μM, about 50 μM, about 60 μM, about 70 μM, about 80 μM, about 90 μM, or about 100 μM. In some aspects, the USP1 inhibitors of the present disclosure bind to a USP1 protein with an affinity of about 100 nM to about 1 μM, about 100 nM to about 900 nM, about 100 nM to about 800 nM, about 100 nM to about 700 nM, about 100 nM to about 600 nM, about 100 nM to about 500 nM, about 100 nM to about 400 nM, about 100 nM to about 300 nM, about 100 nM to about 200 nM, about 200 nM to about 1 μM, about 300 nM to about 1 μM, about 400 nM to about 1 μM, about 500 nM to about 1 μM, about 600 nM to about 1 μM, about 700 nM to about 1 μM, about 800 nM to about 1 μM, about 900 nM to about 1 μM, about 100 nM, about 200 nM, about 300 nM, about 400 nM, about 500 nM, about 600 nM, about 700 nM, about 800 nM, or about 900 nM. In some aspects, the USP1 inhibitors of the present disclosure bind to a USP1 protein with an affinity of about 1 nM to about 100 nM, 1 nM to about 90 nM, 1 nM to about 80 nM, 1 nM to about 70 nM, 1 nM to about 60 nM, 1 nM to about 50 nM, 1 nM to about 40 nM, 1 nM to about 30 nM, 1 nM to about 20 nM, 1 nM to about 10 nM, about 10 nM to about 100 nM, about 20 nM to about 100 nM, about 30 nM to about 100 nM, about 40 nM to about 100 nM, about 50 nM to about 100 nM, about 60 nM to about 100 nM, about 70 nM to about 100 nM, about 80 nM to about 100 nM, about 90 nM to about 100 nM, about 1 nM, about 2 nM, about 3 nM, about 4 nM, about 5 nM, about 6 nM, about 7 nM, about 8 nM, about 9 nM, about 10 nM, about 20 nM, about 30 nM, about 40 nM, about 50 nM, about 60 nM, about 70 nM, about 80 nM, about 90 nM, or about 100 nM.
In some aspects, the USP1 inhibitors of the present disclosure bind to a USP1 protein with an affinity of less than 1 μM, less than 500 nM, less than 100 nM, less than 10 nM, or less than 1 nM. In some aspects, the USP1 inhibitors bind to a USP1 protein with an affinity of less than 1 nM.
In some aspects, the USP1 inhibitors of the present disclosure inhibit USP1 activity with an IC50 of 1 pM to 100 μM, or 1 pM to 1 μM, or 1 pM to 500 nM, or 1 pM to 100 nM. In some aspects, the USP1 inhibitors inhibit USP1 activity with an IC50 of about 1 pM to about 100 μM, about 1 nM to about 100 μM, about 1 μM to about 100 μM, about 1 μM to about 50 μM, about 1 μM to about 40 μM, about 1 μM to about 30 μM, about 1 μM to about 20 μM, or about 1 μM to about 10 μM, about 1 μM, about 5 μM, about 10 μM, about 15 μM, about 20 μM, about 25 μM, about 30 μM, about 35 μM, about 40 μM, about 45 μM, about 50 μM, about 60 μM, about 70 μM, about 80 μM, about 90 μM, or about 100 μM. In some aspects, the USP1 inhibitors inhibit USP1 activity with an IC50 of about 100 nM to about 1 μM, about 100 nM to about 900 nM, about 100 nM to about 800 nM, about 100 nM to about 700 nM, about 100 nM to about 600 nM, about 100 nM to about 500 nM, about 100 nM to about 400 nM, about 100 nM to about 300 nM, about 100 nM to about 200 nM, about 200 nM to about 1 μM, about 300 nM to about 1 μM, about 400 nM to about 1 μM, about 500 nM to about 1 μM, about 600 nM to about 1 μM, about 700 nM to about 1 μM, about 800 nM to about 1 μM, about 900 nM to about 1 μM, about 100 nM, about 200 nM, about 300 nM, about 400 nM, about 500 nM, about 600 nM, about 700 nM, about 800 nM, or about 900 nM.
In some aspects, the USP1 inhibitors of the present disclosure inhibit USP1 activity with an IC50 of about 1 nM to about 100 nM, 1 nM to about 90 nM, 1 nM to about 80 nM, 1 nM to about 70 nM, 1 nM to about 60 nM, 1 nM to about 50 nM, 1 nM to about 40 nM, 1 nM to about 30 nM, 1 nM to about 20 nM, 1 nM to about 10 nM, about 10 nM to about 100 nM, about 20 nM to about 100 nM, about 30 nM to about 100 nM, about 40 nM to about 100 nM, about 50 nM to about 100 nM, about 60 nM to about 100 nM, about 70 nM to about 100 nM, about 80 nM to about 100 nM, about 90 nM to about 100 nM, about 1 nM, about 2 nM, about 3 nM, about 4 nM, about 5 nM, about 6 nM, about 7 nM, about 8 nM, about 9 nM, about 10 nM, about 20 nM, about 30 nM, about 40 nM, about 50 nM, about 60 nM, about 70 nM, about 80 nM, about 90 nM, or about 100 nM. In some aspects, the USP1 inhibitors inhibit USP1 activity with an IC50 of less than 1 μM, less than 500 nM, less than 100 nM, less than 10 nM, or less than 1 nM. In some aspects, the USP1 inhibitors inhibit USP1 activity with an IC50 of less than 1 nM.
Other exemplary USP1 inhibitors are disclosed, for example, in WO 2020/132269 and U.S. Provisional Application 62/857,986, each of which is herein incorporated by reference in its entirety.
Any suitable assay in the art can be used to determine an activity, detect an outcome or effect, or determine efficacy. See, e.g. U.S. application Ser. No. 16/721,079. U.S. application Ser. No. 16/721,079 is herein incorporated by reference in its entirely.
In some instances, a method of determining whether a USP1 inhibitor compound inhibits USP1 deubiquitinase activity measures a change in mass upon di-ubiquitin cleavage of deubiquitinase binding. For example, ubiquitin aldehyde and ubiquitin vinyl sulfone form covalent irreversible linkages to deubiquitinases that result in observable mass changes to the deubiquitinases. Similarly, cleavage of di-ubiquitins results in an observable mass change.
In some instances, a method of determining whether a USP1 inhibitor compound inhibits USP1 deubiquitinase activity involves an increase in luminescence or fluorescence upon cleavage, e.g., that can be monitored on a plate reader. Such assays can use ubiquitin linked to a flurophore through a linker linkage, such as ubiquitin-7-amino-4-methylcoumarin (Ub-AMC) or ubiquitin-Rhodamine110. Such assays can also use a di-ubiquitin containing an isopeptide linkage. Exemplary di-ubiquitins can comprise a flurophore on one ubiquitin and a quencher on the other ubiquitin such that fluorescence increases with then di-ubiquitin is cleaved. Such assays can also use enzyme-coupled systems wherein ubiquitin is coupled to an enzyme that is only active in producing a fluorescence enzyme product when released from the ubiquitin.
In various aspects, the PARP inhibitors of the disclosure reduce the level of one or more PARP proteins and/or inhibit or reduce at least one biological activity of one or more PARP proteins.
PARP inhibitors include, for example, olaparib (Lynparza®), rucaparib (Rubraca®), niraparib (Zejula®), and talazoparib (Talzenna®).
In one aspect, the PARP inhibitor is niraparib (Zejula®), which is sold as niraparib tosylate monohydrate. The chemical name for niraparib tosylate monohydrate is 2-{4-[(3S)-piperidin-3-yl]phenyl}-2Hindazole 7-carboxamide 4-methylbenzenesulfonate hydrate (1:1:1). The molecular formula of niraparib tosylate is C26H30N4O5S, and it has a molecular weight of 492.6 g/mol.
Niraparib is an inhibitor of poly(ADP-ribose) polymerase (PARP) enzymes, PARP-1 and PARP-2, which play a role in DNA repair. In vitro studies have shown that niraparib-induced cytotoxicity may involve inhibition of PARP enzymatic activity and increased formation of PARP-DNA complexes resulting in DNA damage, apoptosis and cell death. Increased niraparib-induced cytotoxicity was observed in tumor cell lines with or without deficiencies in BRCA1/2. Niraparib decreased tumor growth in mouse xenograft models of human cancer cell lines with deficiencies in BRCA1/2 and in human patient-derived xenograft tumor models with homologous recombination deficiency that had either mutated or wild type BRCA1/2.
In another aspect, the PARP inhibitor is olaparib (Lynparza®). The chemical name is 4-[(3-{[4-(cyclopropylcarbonyl)piperazin-1-yl]carbonyl}-4-fluorophenyl)-methyl]phthalazin-1(2H)-one. The molecular formula is C24H23FN4O3, and the molecular weight is 434.5 g/mol.
Olaparib is an inhibitor of poly (ADP-ribose) polymerase (PARP) enzymes, including PARP1, PARP2, and PARP3. Olaparib has been shown to inhibit growth of select tumor cell lines in vitro and decrease tumor growth in mouse xenograft models of human cancer, both as monotherapy or following platinum-based chemotherapy. Increased cytotoxicity and anti-tumor activity following treatment with olaparib were noted in cell lines and mouse tumor models with deficiencies in BRCA and non-BRCA proteins involved in the homologous recombination repair (HRR) of DNA damage and correlated with platinum response. In vitro studies have shown that olaparib-induced cytotoxicity may involve inhibition of PARP enzymatic activity and increased formation of PARP-DNA complexes, resulting in DNA damage and cancer cell death.
In one aspect, the PARP inhibitors are used in anti-cancer combination therapies with USP1 inhibitors of the present disclosure. In addition to the PARP inhibitor and USP1 inhibitor, other therapies can be used either before, during or after the combination therapy.
The present disclosure provides compounds that are active in inhibiting the activity of PARP. Any suitable assay in the art can be used to determine an activity, detect an outcome or effect, or determine efficacy. See, e.g., Dillon, et al., JBS., 8(3), 347-352 (2003); U.S. Pat. No. 9,566,276.
In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of less than about 50 nM, between about 50 nM and about 200 nM, between about 200 nM and about 2 pM, or greater than 2 pM.
In some aspects, the PARP inhibitors of the disclosure bind to a PARP protein with an affinity in the range of 1 pM to 100 μM, or 1 pM to 1 μM, or 1 pM to 500 nM, or 1 pM to 100 nM. In some aspects, the PARP inhibitors of the disclosure bind to a PARP protein with an affinity of about 1 pM to about 100 μM, about 1 nM to about 100 μM, about 1 μM to about 100 μM, about 1 μM to about 50 μM, about 1 μM to about 40 μM, about 1 μM to about 30 μM, about 1 μM to about 20 μM, or about 1 μM to about 10 μM, about 1 μM, about 5 μM, about 10 μM, about 15 μM, about 20 μM, about 25 μM, about 30 μM, about 35 μM, about 40 μM, about 45 μM, about 50 μM, about 60 μM, about 70 μM, about 80 μM, about 90 μM, or about 100 μM. In some aspects, the PARP inhibitors of the disclosure bind to a PARP protein with an affinity of about 100 nM to about 1 μM, about 100 nM to about 900 nM, about 100 nM to about 800 nM, about 100 nM to about 700 nM, about 100 nM to about 600 nM, about 100 nM to about 500 nM, about 100 nM to about 400 nM, about 100 nM to about 300 nM, about 100 nM to about 200 nM, about 200 nM to about 1 μM, about 300 nM to about 1 μM, about 400 nM to about 1 μM, about 500 nM to about 1 μM, about 600 nM to about 1 μM, about 700 nM to about 1 μM, about 800 nM to about 1 μM, about 900 nM to about 1 μM, about 100 nM, about 200 nM, about 300 nM, about 400 nM, about 500 nM, about 600 nM, about 700 nM, about 800 nM, or about 900 nM. In some aspects, the PARP inhibitors of the disclosure bind to a PARP protein with an affinity of about 1 nM to about 100 nM, 1 nM to about 90 nM, 1 nM to about 80 nM, 1 nM to about 70 nM, 1 nM to about 60 nM, 1 nM to about 50 nM, 1 nM to about 40 nM, 1 nM to about 30 nM, 1 nM to about 20 nM, 1 nM to about 10 nM, about 10 nM to about 100 nM, about 20 nM to about 100 nM, about 30 nM to about 100 nM, about 40 nM to about 100 nM, about 50 nM to about 100 nM, about 60 nM to about 100 nM, about 70 nM to about 100 nM, about 80 nM to about 100 nM, about 90 nM to about 100 nM, about 1 nM, about 2 nM, about 3 nM, about 4 nM, about 5 nM, about 6 nM, about 7 nM, about 8 nM, about 9 nM, about 10 nM, about 20 nM, about 30 nM, about 40 nM, about 50 nM, about 60 nM, about 70 nM, about 80 nM, about 90 nM, or about 100 nM. In some aspects, the PARP inhibitors of the disclosure bind to a PARP protein with an affinity of less than 1 μM, less than 500 nM, less than 100 nM, less than 10 nM, or less than 1 nM. In some aspects, the PARP inhibitors of the disclosure bind to a PARP protein with an affinity of less than 1 nM.
In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of 1 pM to 100 μM, or 1 pM to 1 μM, or 1 pM to 500 nM, or 1 pM to 100 nM. In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of about 1 pM to about 100 μM, about 1 nM to about 100 μM, about 1 μM to about 100 μM, about 1 μM to about 50 μM, about 1 μM to about 40 μM, about 1 μM to about 30 μM, about 1 μM to about 20 μM, or about 1 μM to about 10 μM, about 1 μM, about 5 μM, about 10 μM, about 15 μM, about 20 μM, about 25 μM, about 30 μM, about 35 μM, about 40 μM, about 45 μM, about 50 μM, about 60 μM, about 70 μM, about 80 μM, about 90 μM, or about 100 μM. In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of about 100 nM to about 1 μM, about 100 nM to about 900 nM, about 100 nM to about 800 nM, about 100 nM to about 700 nM, about 100 nM to about 600 nM, about 100 nM to about 500 nM, about 100 nM to about 400 nM, about 100 nM to about 300 nM, about 100 nM to about 200 nM, about 200 nM to about 1 μM, about 300 nM to about 1 μM, about 400 nM to about 1 μM, about 500 nM to about 1 μM, about 600 nM to about 1 μM, about 700 nM to about 1 μM, about 800 nM to about 1 μM, about 900 nM to about 1 μM, about 100 nM, about 200 nM, about 300 nM, about 400 nM, about 500 nM, about 600 nM, about 700 nM, about 800 nM, or about 900 nM. In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of about 1 nM to about 100 nM, 1 nM to about 90 nM, 1 nM to about 80 nM, 1 nM to about 70 nM, 1 nM to about 60 nM, 1 nM to about 50 nM, 1 nM to about 40 nM, 1 nM to about 30 nM, 1 nM to about 20 nM, 1 nM to about 10 nM, about 10 nM to about 100 nM, about 20 nM to about 100 nM, about 30 nM to about 100 nM, about 40 nM to about 100 nM, about 50 nM to about 100 nM, about 60 nM to about 100 nM, about 70 nM to about 100 nM, about 80 nM to about 100 nM, about 90 nM to about 100 nM, about 1 nM, about 2 nM, about 3 nM, about 4 nM, about 5 nM, about 6 nM, about 7 nM, about 8 nM, about 9 nM, about 10 nM, about 20 nM, about 30 nM, about 40 nM, about 50 nM, about 60 nM, about 70 nM, about 80 nM, about 90 nM, or about 100 nM. In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of less than 1 μM, less than 500 nM, less than 100 nM, less than 10 nM, or less than 1 nM. In some aspects, the PARP inhibitors of the disclosure inhibit PARP activity with an IC50 of less than 1 nM.
As demonstrated herein, cancers comprising cells with elevated levels of RAD51 are sensitive to USP1 inhibitors and/or combinations of USP1 inhibitors and PARP inhibitors. The elevated levels of RAD51 can be elevated RAD51 protein levels, elevated RAD51 protein foci levels, and/or elevated RAD51 mRNA level.
Various methods of identifying a cancer as a USP1 inhibitor-sensitive cancer and/or a cancer that is sensitive to the combination of USP1 inhibitors and PARP inhibitors are provided herein. In some instances, such methods comprise detecting RAD51 (e.g., RAD51 protein, RAD51 protein foci, and/or RAD51 mRNA) levels in cancer cells (e.g., using a sample obtained from the cancer). RAD51 protein levels can be detected using, for example, immunofluorescence, western blots, fluorescence-activated cell sorting (FACS), and/or immunohistochemistry. RAD51 mRNA levels can be detected, for example, using quantitative reverse transcriptase (RT)-polymerase chain reaction (PCR). Elevated levels of RAD51 protein and/or mRNA indicate that a cancer is sensitive to USP1 inhibitors or to combinations of USP1 inhibitors and PARP inhibitors.
Methods of detecting RAD51 and RAD51 protein foci are provided, for example, in Castroviejo-Bermejo, Marta, et al., EMBO Molecular Medicine 10(12):e9172 (2018), which is herein incorporated by reference in its entirety. RAD51 can be detected, for example, using immunofluorescence. RAD51 foci, e.g., of 0.42-1.15 μm diameter can be quantified on formalin-fixed paraffin embedded (FFPE) tumor samples, by scoring the percentage of cells in the S/G2-cell cycle phase (e.g., geminin-positive cells) with 5 or more RAD51 nuclear foci. In some aspects, cancers comprising cells with elevated levels of RAD51 are cancers wherein at least 10% of cells that are in the S/G2 phase of the cell cycle (e.g., geminin-positive cells) are RAD51-positive.
In some aspects, a method of selecting a subject with cancer for treatment with a USP1 inhibitor (optionally in combination with a PARP inhibitor) comprises determining whether the cancer comprises cells with elevated levels of RAD51, wherein if the cancer comprises cells with elevated levels of RAD51, the subject is selected for treatment with a USP1 inhibitor, optionally in combination with a PARP inhibitor.
A cancer with elevated levels of RAD51 can be a homologous-recombination deficient cancer. A cancer with elevated levels of RAD51 can be a BRCA1 mutant cancer. A cancer with elevated levels of RAD51 can be a BRCA2 mutant cancer. A cancer with elevated levels of RAD51 can be a BRCA1 mutant and BRCA2 mutant cancer. A cancer with elevated levels of RAD51 can be cancer with deleterious or suspected deleterious mutations in BRCA1 and BRCA2 genes and/or a positive Genomic Instability Score, e.g., as determined using myChoice® CDx (Myriad®).
Since combinations of the disclosure are inhibitors of USP1 proteins and PARP proteins, the present disclosure provides a method for inhibiting a USP1 protein and/or a PARP protein comprising contacting a USP1 and/or PARP protein or a composition comprising a USP1 and/or PARP protein with one or more combinations of the disclosure.
Since combinations of the disclosure are inhibitors of USP1 and PARP proteins, a number of diseases, conditions, or disorders mediated by USP1 and/or PARP proteins can be treated by employing these compounds. The present disclosure is thus directed generally to a method for treating a disease, condition, or disorder responsive to the inhibition of USP1 and/or PARP proteins in an animal suffering from, or at risk of suffering from, the disorder, the method comprising administering to the animal an effective amount of one or more combinations of the disclosure.
The present disclosure is further directed to a method of inhibiting USP1 and/or PARP proteins in an animal in need thereof, the method comprising administering to the animal a therapeutically effective amount of a combination of the disclosure.
In some aspects, the combinations of the disclosure can be used to inhibit the activity of a USP1 and/or PARP protein. For example, in some aspects, a method of inhibiting a USP1 and/or PARP protein comprises contacting the USP1 and/or PARP protein with a combination of the disclosure. The contacting can occur in vitro or in vivo.
In some aspects, the combinations of the disclosure can be used to treat a USP1 and/or PARP protein mediated disorder. A USP1 and/or PARP protein mediated disorder is any pathological condition in which a USP1 and/or PARP protein is known to play a role. In some aspects, a USP1 and/or PARP mediated disorder is a proliferative disease such as cancer. In some aspects, the combinations of the disclosure can delay, reduce, or prevent rebounding (rapid re-growth) of a tumor. In some aspects, the combination of the disclosure is not significantly more toxic than the USP1 inhibitor alone. In some aspects, the combination of the disclosure is not significantly more toxic than the PARP inhibitor alone. In some aspects, the combination of the disclosure is not significantly more toxic than either the USP1 inhibitor alone or the PARP inhibitor alone.
In some aspects, the combination of the disclosure is less toxic than the PARP inhibitor alone. Accordingly, in some aspects, the present disclosure provides a method of treating cancer in a subject who previously received treatment with a first poly ADP-ribose polymerase (PARP) inhibitor, the method comprising administering to the subject a ubiquitin-specific-processing protease (USP1) inhibitor and a second PARP inhibitor, wherein the first and the second PARP inhibitors are the same or different PARP inhibitors. The treatment with the first PARP inhibitor may have been interrupted or discontinued, e.g., as a result of unacceptable toxicity and/or unacceptable adverse reactions. Exemplary toxicities or adverse reactions include hematological toxicity such as thrombocytopenia, anemia, or neutropenia, pneumonitis, dyspnea, fever, cough, wheezing, a radiological abnormality, hypertension, myelodysplastic syndrome/acute myeloid leukemia (MDS/AML), nausea, and/or fatigue.
In some aspects, the interruption of the treatment with the first PARP inhibitor was for at least one week, optionally from one week to four weeks. In some aspects, the interruption was for at least two weeks, optionally from two weeks to four weeks. In some aspects, the interruption was for at least three weeks, optionally from three weeks to four weeks. In some aspects, the interruption was for at least four weeks. In some aspects, the interruption was for no more than four weeks.
In some aspects, the dose of the first PARP inhibitor was reduced, for example, reduced to one quarter, one third, one half, two thirds, or three quarters of the dose prior to the reduction. The first PARP inhibitor can be olaparib, and the dose prior to the reduction can be 400 mg taken twice daily. Such a dose can be reduced, e.g., to 200 mg taken twice daily or 100 mg taken twice daily. The first PARP inhibitor can be niraparib, and the dose prior to the reduction can be 300 mg taken once daily. Such a dose can be reduced, e.g., to 200 mg taken once daily or 100 mg taken once daily. The first PARP inhibitor can be talazoparib, and the dose prior to the reduction can be 1 mg taken once daily. Such a dose can be reduced, e.g., to 0.75 mg taken once daily, 0.5 mg taken once daily, or 0.25 mg taken once daily. The first PARP inhibitor can be rucaparib, and the dose prior to the reduction can be 600 mg taken once daily. Such a dose can be reduced, e.g., to 500 mg taken twice daily, 400 mg taken twice daily, or 300 mg taken twice daily.
Various methods of treating diseases and disorders with the combinations of the disclosure are provided herein. Exemplary diseases and disorders that may be treated with the combinations of the disclosure include, but are not limited to, cancer.
In some aspects, methods of treating cancer with combinations of the disclosure are provided. Such methods comprise administering to a subject with cancer a therapeutically effective amount of a combination of the disclosure.
In some aspects, the cancer to be treated with a combination of the disclosure is selected from a hematological cancer, a lymphatic cancer, and a DNA damage repair pathway deficient cancer. In some aspects, the cancer to be treated with a combination of the disclosure is a cancer that comprises cancer cells with a mutation in a gene encoding p53. In some aspects, the cancer to be treated with a combination of the disclosure is a cancer that comprises cancer cells with a loss of function mutation in a gene encoding p53. In some aspects, the cancer to be treated with a combination of the disclosure is a cancer that comprises cancer cells with a mutation in a gene encoding BRCA1. In some aspects, the cancer to be treated with a combination of the disclosure is a cancer that comprises cancer cells with a mutation in a gene encoding BRCA2. In some aspects, the cancer to be treated with a combination of the disclosure is a cancer that comprises cancer cells with a loss of function mutation in a gene encoding ATM.
In some aspects, the cancer to be treated with a combination of the disclosure is selected from non-small cell lung cancer (NSCLC), osteosarcoma, ovarian cancer, and breast cancer. In some aspects, the cancer is uterine cancer. In some aspects, the cancer is peritoneal cancer. In some aspects, the cancer is endometrial cancer, In some aspects, the cancer is ovarian cancer or breast cancer. In some aspects, the cancer is ovarian cancer. In some aspects, the cancer is breast cancer. In some aspects, the cancer is a triple negative breast cancer. In some aspects, the cancer is an ovarian cancer. In some aspects, the ovarian cancer is a BRCA1 mutant cancer, a BRCA2 mutant cancer, or a p53 mutant cancer. In some aspects, the ovarian cancer is a BRCA1 mutant cancer and a p53 mutant cancer. In some aspects, the ovarian cancer is a BRCA1 and BRCA2 mutant cancer. In some aspects, the ovarian cancer is a BRCA2 mutant cancer.
In some aspects, the cancer to be treated with a combination of the disclosure is a cancer that comprises cancer cells with elevated levels of RAD51. The elevated levels of RAD51 can be elevated RAD51 protein levels, elevated RAD51 protein foci levels, and/or elevated levels RAD51 mRNA levels. In some aspects, a cancer that comprises cancer cells with elevated levels of RAD51 refers to a cancer wherein at least 10% of cells that are in the S/G2 phase of the cell cycle (e.g., geminin-positive cells) in a sample obtained from the cancer are RAD51-positive (e.g., contain 5 or more RAD51 nuclear foci).
A cancer with elevated levels of RAD51 can be a homologous-recombination deficient cancer. A cancer with elevated levels of RAD51 can be a BRCA1 mutant cancer, a BRCA2 mutant cancer, or a BRCA1 and BRCA2 mutant cancer. A cancer with elevated levels of RAD51 can be cancer with deleterious or suspected deleterious mutations in BRCA1 and BRCA2 genes and/or a positive Genomic Instability Score, e.g., as determined using myChoice® CDx (Myriad®).
In some aspects, the cancer to be treated with a combination of the disclosure is selected from the group consisting of bone cancer, including osteosarcoma and chondrosarcoma; brain cancer, including glioma, glioblastoma, astrocytoma, medulloblastoma, and meningioma; soft tissue cancer, including rhabdoid and sarcoma; kidney cancer; bladder cancer; skin cancer, including melanoma; and lung cancer, including non-small cell lung cancer; colon cancer, uterine cancer; nervous system cancer; head and neck cancer; pancreatic cancer; and cervical cancer. In some aspects, the cancer to be treated with a combination of the disclosure is selected from the group consisting of uterine cancer, peritoneal cancer, and endometrial cancer.
Various methods of treating cancer with a combination of the disclosure are provided herein. In some aspects, a therapeutically effective amount of a combination of the disclosure is administered to a subject with cancer.
In some aspects, such methods comprise (a) identifying a cancer in a subject as a USP1 and/or PARP inhibitor-sensitive cancer and then (b) administering a therapeutically effective amount of a combination of the disclosure to the subject.
In some aspects, such methods comprise administering to a subject with triple negative breast cancer a therapeutically effective amount of a combination of the disclosure.
In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is a homologous-recombination deficient cancer. In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer comprises cancer cells with a mutation in a gene encoding p53. In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer comprises cancer cells with a loss of function mutation in a gene encoding p53. In some aspects, a combination of the disclosure is used to treat a cancer that does not have a defect in the homologous recombination pathway.
In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is a BRCA1 mutant cancer. In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is a BRCA2 mutant cancer. In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is a BRCA1 mutant cancer and a BRCA2 mutant cancer. In some aspects, the cancer is not a BRCA1 mutant cancer or a BRCA2 mutant cancer. In some aspects, the cancer is a BRCA1 deficient cancer. In some aspects, the cancer is a BRCA2 deficient cancer. In some aspects, the cancer is a BRCA1 deficient cancer and a BRCA2 mutant cancer.
In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is an ATM mutant cancer. In some aspects, the cancer is not an ATM mutant cancer. In some aspects, the cancer is an ATM deficient cancer.
In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is a PARP inhibitor resistant or refractory cancer. In some aspects, a combination of the disclosure is used to treat a cancer, wherein the cancer is a PARP inhibitor resistant or refractory BRCA1-deficient cancer.
In some aspects, the cancer is a BRCA1 and/or BRCA2 mutant cancer, wherein the cancer comprises cells with elevated levels of RAD18, e.g., wherein the elevated levels of RAD18 are at least as high as the RAD18 protein and/or mRNA levels in ES2 cells (ES2 cells are publicly available, for example from the American Type Culture Collection (ATCC; CRL-1978)) or wherein the elevated levels of RAD18 are higher than the RAD18 protein and/or mRNA levels in HEP3B217 cells (HEP3B217 cells are publicly available, for example, from the ATCC (HB-8064)). In some aspects, a triple negative breast cancer is a BRCA1 and/or BRCA2 mutant cancer.
In some aspects, the cancer is a that comprises cancer cells with elevated levels of RAD51, e.g., elevated RAD51 protein levels, elevated RAD51 protein foci levels, and/or elevated RAD51 mRNA levels. In some aspects, a cancer that comprises cancer cells with elevated levels of RAD51 refers to a cancer wherein at least 10% of cells that are in the S/G2 phase of the cell cycle (e.g., geminin-positive cells) in a sample obtained from the cancer are RAD51-positive (e.g., contain 5 or more RAD51 nuclear foci).
In some instances, the cancer is a solid cancer. In some instances, the cancer is a hematological/lymphatic cancer. In some instances, the cancer is a DNA damage repair pathway deficient cancer. In some instances, the cancer is a homologous-recombination deficient cancer. In some instances, the cancer comprises cancer cells with a mutation in a gene encoding p53. In some instances, the cancer comprises cancer cells with a loss of function mutation in a gene encoding p53. In some instances, the cancer is selected from the group consisting of non-small cell lung cancer (NSCLC), osteosarcoma, ovarian cancer, and breast cancer (including triple negative breast cancer). In some instances, the cancer is ovarian cancer or breast cancer (including triple negative breast cancer). In some instances, the cancer is ovarian cancer. In some instances, the cancer is breast cancer (including triple negative breast cancer.) In some instances, the cancer is uterine cancer. In some instances, the cancer is peritoneal cancer. In some instances, the cancer is endometrial cancer.
In some aspects, a combination of the disclosure is used in combination with one or more additional therapeutic agents to treat cancer.
In some aspects, provided herein are combinations of the disclosure for use as a medicament or for use in preparing a medicament, e.g., for the treatment of cancer. In some aspects, provided herein are combinations of the disclosure for use in a method for the treatment of cancer.
In some aspects, methods of treating cancers comprising cells with elevated levels of RAD51 are provided. Cancers comprising cells with elevated levels of RAD51 can be referred to herein as “RAD51 high cancers.” Such methods comprise administering to a subject with a RAD51 high cancer a therapeutically effective amount of a USP1 inhibitor or a combination of a USP1 inhibitor and a PARP inhibitor.
In some aspects, the RAD51 high cancer to be treated with a USP1 inhibitor or a combination of a USP1 inhibitor and a PARP inhibitor is selected from a hematological cancer, a lymphatic cancer, and a DNA damage repair pathway deficient cancer. In some aspects, the RAD51 high cancer to be treated with a USP1 inhibitor or a combination of a USP1 inhibitor and a PARP inhibitor is a homologous-recombination deficient cancer.
In some aspects, the RAD51 high cancer to be treated with USP1 inhibitor or a combination of a USP1 inhibitor and a PARP inhibitor is selected from non-small cell lung cancer (NSCLC), osteosarcoma, ovarian cancer, and breast cancer. In some aspects, the cancer is uterine cancer. In some aspects, the RAD51 high cancer is peritoneal cancer. In some aspects, the RAD51 high cancer is endometrial cancer. In some aspects, the RAD51 high cancer is ovarian cancer or breast cancer. In some aspects, the RAD51 high cancer is ovarian cancer. In some aspects, the RAD51 high cancer is breast cancer. In some aspects, the RAD51 high cancer is a triple negative breast cancer. In some aspects, the RAD51 high cancer is an ovarian cancer.
In some aspects, the RAD51 high cancer to be treated with USP1 inhibitor or a combination of a USP1 inhibitor and a PARP inhibitor is selected from the group consisting of bone cancer, including osteosarcoma and chondrosarcoma; brain cancer, including glioma, glioblastoma, astrocytoma, medulloblastoma, and meningioma; soft tissue cancer, including rhabdoid and sarcoma; kidney cancer; bladder cancer; skin cancer, including melanoma; and lung cancer, including non-small cell lung cancer; colon cancer, uterine cancer; nervous system cancer; head and neck cancer; pancreatic cancer; and cervical cancer. In some aspects, the RAD51 high cancer to be treated with a USP1 inhibitor or a combination of a USP1 inhibitor and a PARP inhibitor is selected from the group consisting of uterine cancer, peritoneal cancer, and endometrial cancer.
Various methods of treating a RAD51 high cancer with a combination of the disclosure are provided herein. In some aspects, a therapeutically effective amount of a combination of the disclosure is administered to a subject with a RAD51 high cancer.
In some aspects, such methods comprise (a) detecting levels of RAD51 (e.g., RAD51 protein and/or RAD51 mRNA) in cancer cells (e.g., in a cancer sample obtained from the subject) and then (b) administering a therapeutically effective amount of a USP1 inhibitor to a subject have a cancer comprising cells with elevated levels of RAD51. In some aspects, such methods comprise (a) detecting levels of RAD51 (e.g., RAD51 protein and/or RAD51 mRNA) in cancer cells (e.g., in a cancer sample obtained from the subject) and then (b) administering a USP1 inhibitor in combination with a PARP inhibitor to a subject have a cancer comprising cells with elevated levels of RAD51.
Combinations of the disclosure can be administered to a mammal in the form of a raw chemicals without any other components present, or combinations of the disclosure can also be administered to a mammal as part of a pharmaceutical composition containing the compound combined with a suitable pharmaceutically acceptable carrier (see, for example, Gennaro, Remington: The Science and Practice of Pharmacy with Facts and Comparisons: Drugfacts Plus, 20th ed. (2003); Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems, 7th ed., Lippencott Williams and Wilkins (2004); Kibbe et al., Handbook of Pharmaceutical Excipients, 3rd ed., Pharmaceutical Press (2000)). Such a carrier can be selected from pharmaceutically acceptable excipients and auxiliaries. The term “pharmaceutically acceptable carrier” or “pharmaceutically acceptable vehicle” encompasses any of the standard pharmaceutical carriers, solvents, surfactants, or vehicles. Standard pharmaceutical carriers and their formulations are described in Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa., 19th ed. 1995.
A pharmaceutical combination composition of the present disclosure may be prepared as liquid suspensions or solutions using a liquid, such as an oil, water, an alcohol, and combinations of these.
The pharmaceutical combination compositions to be used for in vivo administration can be sterile. This is readily accomplished by filtration through, e.g., sterile filtration membranes.
Pharmaceutical combination compositions within the scope of the present disclosure include all compositions where a USP1 inhibitor and a PARP inhibitor of the disclosure are combined with one or more pharmaceutically acceptable carriers. In one embodiment, the USP1 inhibitor and PARP inhibitor of the disclosure are present in the composition in an amount that is effective to achieve its intended therapeutic purpose.
A pharmaceutical combination composition of the present disclosure can be administered to any patient that may experience the beneficial effects of a combination of the disclosure. Foremost among such patients are mammals, e.g., humans and companion animals, although the disclosure is not intended to be so limited. In one aspect, the patient is a human. In another aspect, a pharmaceutical combination composition of the present disclosure can be administered to a patient having PARP inhibitor resistant or refractory cancer. In another embodiment, a pharmaceutical combination composition of the present disclosure can be administered to a patient having PARP inhibitor resistant or refractory BRCA1-deficient cancer. In another embodiment, a pharmaceutical combination composition of the present disclosure can be administered to a patient having a cancer that comprises cancer cells with elevated levels of RAD51, e.g., elevated RAD51 protein levels, elevated RAD51 protein foci levels, and/or elevated RAD51 mRNA levels. In some aspects, a pharmaceutical combination composition of the present disclosure can be administered to a patient having a cancer wherein at least 10% of cells that are in the S/G2 phase of the cell cycle (e.g., geminin-positive cells) in a sample obtained from the cancer are RAD51-positive (e.g., contain 5 or more RAD51 nuclear foci).
In another embodiment, the present disclosure provides kits that comprise a combination of the disclosure packaged in a manner that facilitates their use to practice methods of the present disclosure. In one embodiment, the kit includes a USP1 inhibitor and a PARP inhibitor of the disclosure packaged in a container, such as a sealed bottle or vessel, with a label affixed to the container or included in the kit that describes use of the compounds to practice the methods of the disclosure. In one embodiment, the combination composition is packaged in a unit dosage form. The kit further can include a device suitable for administering the combination composition according to the intended route of administration. In some aspects, the present disclosure provides a kit that comprises a USP1 inhibitor and a PARP inhibitor of the disclosure, or a pharmaceutically acceptable salt or solvate thereof, and instructions for administering the compounds, or pharmaceutically acceptable salts or solvates thereof, to a patient having cancer.
In some aspects, the present disclosure provides a pharmaceutical combination composition comprising a USP1 inhibitor and a PARP inhibitor of the disclosure, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier.
In some aspects, the present disclosure provides a pharmaceutical combination composition comprising a USP1 inhibitor and a PARP inhibitor of the disclosure, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier, wherein the combination binds to a protein encoded by the USP1 gene and/or a PARP gene.
In some aspects, the present disclosure provides a pharmaceutical combination composition comprising a USP1 inhibitor and a PARP inhibitor of the disclosure, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier, wherein the pharmaceutical composition is for use in treating cancer.
In some aspects, the present disclosure provides a pharmaceutical combination composition comprising a USP1 inhibitor and a PARP inhibitor of the disclosure, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable carrier, wherein the pharmaceutical composition is for the manufacture of a medicament for treatment of cancer.
In vitro experiments were conducted using the colony formation unit (CFU) assay on various cell lines. The CFU assay involved first establishing what cell plating density enabled the development of clearly interspersed colonies on a six-well plate when left to grow for around 14 days. Once this density had been identified, cells were plated on day −1 and on day 0, the wells were treated with DMSO or increasing concentrations of USP1 inhibitor or Niraparib (3 nM, 10 nM, 30 nM, 100 nM, and 300 nM), or increasing concentrations of USP1 inhibitor or Olaparib (3 nM, 10 nM, 30 nM, 100 nM, and 300 nM). Media was changed on day 8 containing appropriate concentrations of DMSO, USP1 inhibitor, Niraparib, or Olaparib. At or around day 14 when clearly interspersed colonies were visible in the DMSO treated wells, the cells were fixed and stained using 0.1% crystal violet in 10% ethanol for 20 minutes at room temperature. The plates were imaged then the amount of crystal violet stain in each well was quantified by extracting the crystal violet into 10% acetic acid and the absorbance measured at 565 nm. The CFU results are shown in Table 1 and Table 2.
The results in Table 1 showed synergy was detected in cell lines having an enrichment for BRCA1 loss of function mutations or possible loss of function mutations, suggesting that patients with such mutations may benefit from a combination therapy of a USP1 inhibitor and a PARP inhibitor. For example, out the total number of BRCA1 mutant cell lines ran in the CFU assays, 8 out of 9 cell lines showed synergy above a cutoff score of 6.
IC50 values were calculated by fitting a two-parameter hill equation to the dose-response measurements. Non-linear-least-squares was used to find parameter values that minimize the squared error of the model fit to measured dose response. Non-linear-least-squares estimation was performed using the minpack.lm R package, version 1.2-1. Bliss synergy scores were calculated using the synergyfinder R package version 1.6.1.
Mutation in ATM, BRCA1, and BRCA2 were determined using an in-house pipeline. CCLE RNA-seq data was analyzed by the GATK tool MuTect2 version 3.7-0-g56f2c1a, to identify variants, then classified using the GATK tool Funcotator version 3.7-0-g56f2c1a. Funcotator classified variant calls as one of “silent”, “missense”, “splice-site”, “non-sense”, or “frameshift”. An automatic mutation that was classified as splice-site, non-sense or frameshift mutations were manually reviewed. The manual review assessed whether the mutation was homozygous, and whether the call could be attributed to sequencing or variant-calling artifacts such as low sequencing depth or indels located in homopolymer sequences, and summarized the impact on the gene when multiple events were called for a single gene. The output of the manual mutation reviews was a classification of the impact on gene function as one of “loss-of-function”, “possible-loss-of-function”, or “wild-type”. The mutation calls for TP53 were extracted from the CCLE_mutations.csv file, downloaded from depmap.org.
Five patient-derived xenograft models were selected based on availability and with a variety of BRCA and HRD mutational signatures. PARP inhibitor (PARPi) activity was known in selected models based on historical clinical data and from internally-generated data from XenTech SAS. Based on this historical data, a range of PARPi responsive and non-responsive models were chosen. Table 3 shows a summary of the models chosen for testing, single agent activity with the compound of Formula I, and combination activity with the compound of Formula I and Olaparib.
Anti-tumor activity of the USP1 inhibitor of Formula I free base in comparison to Olaparib and Niraparib was evaluated in mice using the MDA-MB-436 subcutaneous human breast tumor model. 7-9 week old female NOD SCID mice from Beijing Anikeeper Biotech Co. Ltd were injected subcutaneously with 10×106 MDA-MB-436 tumor cells. When tumors reached a volume of approximately 200 mm3 mice were randomized into groups of 10 and dosed via oral gavage with either control, Niraparib (50 mg/kg), Olaparib (75 mg/kg) or the USP1 inhibitor of Formula I at either 30, 100 or 300 mg/kg once daily or 30 mg/kg BID twice daily for 28 days. Body weight and tumor volume was measured twice per week. Tumor volume was calculated as mean and standard error of the mean for each treatment group. The percentage tumor growth inhibition (TGI) was calculated using the mean tumor volume from the treatment group on day 28-day 0/mean tumor volume from control treated group on day 28-day 0 where day 0 is the first day of treatment.
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Anti-tumor activity of the USP1 inhibitor of Formula I co-crystal in comparison to Olaparib and Niraparib was evaluated in mice using the MDA-MB-436 subcutaneous human breast tumor model. 7-9 week old female NOD SCID mice from Beijing Anikeeper Biotech Co. Ltd were injected subcutaneously with 10×106 MDA-MB-436 tumor cells. When tumors reached a volume of approximately 200 mm3 mice were randomized into groups of 10 and dosed via oral gavage once daily for 28 days with either control, Niraparib (50 mg/kg), Olaparib (100 mg/kg) or the USP1 inhibitor of Formula I at either 10, 30, 100 or 300 mg/kg. Body weight and tumor volume was measured twice per week. Tumor volume was calculated as mean and standard error of the mean for each treatment group. The percentage tumor growth inhibition (TGI) was calculated using the mean tumor volume from the treatment group on day 28-day 0/mean tumor volume from control treated group on day 28-day 0 where day 0 is the first day of treatment.
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Anti-tumor activity of the USP1 inhibitor of Formula I co-crystal in combination with Olaparib was evaluated in mice using the MDA-MB-436 subcutaneous human breast tumor model. 7-9 week old female NOD SCID mice from Beijing Anikeeper Biotech Co. Ltd were injected subcutaneously with 10×106 MDA-MB-436 tumor cells. When tumors reached a volume of approximately 200 mm3, mice were randomized into groups of 10 for control, Formula I (100 mg/kg) alone and Formula I (30 mg/kg) alone; or 5 mice for Olaparib (50 mg/kg) alone, Formula I (100 mg/kg) and Olaparib (50 mg/kg) combination group and Formula I (30 mg/kg) and Olaparib (50 mg/kg) combination group. Mice were dosed the relevant treatment via oral gavage once daily for 28 days.
Body weight and tumor volume was measured twice per week. Tumor volume was calculated as mean and standard error of the mean for each treatment group. The percentage tumor growth inhibition (TGI) was calculated using the mean tumor volume from the treatment group on day 28-day 0/mean tumor volume from control treated group on day 28-day 0 where day 0 is the first day of treatment.
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Repeat studies assessing the anti-tumor activity of the USP1 inhibitor of Formula I co-crystal in combination with Olaparib in mice using the MDA-MB=436 subcutaneous human breast tumor model. 7-9 week old female NOD SCID mice from Beijing Anikeeper Biotech Co. Ltd were injected subcutaneously with 10×106 MDA-MB-436 tumor cells. When tumors reached a volume of approximately 200 mm3, mice were randomized into groups of 10 and dosed daily (qd) for control, Formula I (100 mg/kg) alone, Formula I (300 mg/kg) alone, Olaparib (50 mg/kg) alone, Olaparib (100 mg/kg) alone or combination groups of Formula I (100 mg/kg) and Olaparib (50 mg/kg), Formula I (100 mg/kg) and Olaparib (100 mg/kg), Formula I (300 mg/kg) and Olaparib (50 mg/kg); or 6 mice dosed twice daily (BID) for Formula I (100 mg/kg BID) alone, Formula I (100 mg/kg BID) and Olaparib (50 mg/kg) combination group. Mice were dosed the relevant treatment via oral gavage either once daily or twice daily (BID) as highlighted above for 28 days.
Body weight and tumor volume was measured twice per week. Tumor volume was calculated as mean and standard error of the mean for each treatment group. The percentage tumor growth inhibition (TGI) was calculated using the mean tumor volume from the treatment group on day 27-day 0/mean tumor volume from control treated group on day 27-day 0 where day 0 is the first day of treatment.
In all groups containing 10 mice, on day 28 of dosing 6 mice per group were euthanized for ex vivo sample analysis. The remaining 4 mice per group were monitored for response post dose termination.
The data in
Anti-tumor activity of the USP1 inhibitor of Formula I co-crystal in combination with Olaparib was evaluated in mice using a variety of patient-derived breast xenograft models in nude mice, as shown in
The data in
Anti-tumor activity of the USP1 inhibitor of Formula I co-crystal in combination with Olaparib was evaluated in the HBCx-11 BRCA1 mutant HRD high human breast PDX model, as shown in
The data in
Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using a variety of patient-derived breast xenograft models in nude mice, as shown in
The data in
Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using three patient-derived ovarian xenograft models in nude mice, as shown in
The data in
Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using three patient-derived ovarian xenograft models in nude mice, as shown in
The data in
Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib is evaluated in mice using three patient-derived ovarian xenograft models in nude mice. 6-8 week old female athymic nude mice from Engivo are anesthetized and a 125 mm3 tumor fragment is placed subcutaneously via incision in the flank. When tumors establish to a tumor volume ranging from approximately 130 to 240 mm3 mice are randomized into groups of 3 and were assigned into the following groups: control, Formula I (100 mg/kg), Formula I (300 mg/kg), Olaparib (50 mg/kg), Olaparib (100 mg/kg) or Formula I (100 mg/kg) and Olaparib (50 mg/kg) combination. Compound is administered via oral gavage once daily for 20 days. Body weight and tumor volume are measured twice per week. Tumor volume is calculated as mean and standard error of the mean for each treatment group. A tumor regression (REG) is defined as a tumor with a smaller volume on the last day of the study as compared to the first day of dosing, and a complete regression (CR) is defined as no palpable tumor at the end of the study.
Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using three patient-derived ovarian xenograft models in nude mice. 6-8 week old female athymic nude mice from Engivo were anesthetized and a 60 mm3 tumor fragment was placed subcutaneously via incision in the flank. When tumors reached a tumor volume ranging from approximately 100 to 180 mm3, mice were randomized into groups of 3-4 mice and were assigned into the following groups: control (vehicle), Formula I (100 mg/kg), Formula I (300 mg/kg), Olaparib (100 mg/kg) or Formula I (100 mg/kg) and Olaparib (100 mg/kg) combination. Compound was administered via oral gavage once daily for 18 days (days 0 to 17). Body weight and tumor volume were measured twice per week. Tolerability was assessed by calculating body weight changes as percent (%) from body weight on day of treatment start (day 0). Tumor volume was calculated as mean and standard error of the mean for each treatment group. A tumor regression (REG) was defined as a tumor with a smaller volume on the last day of the study as compared to the first day of dosing, and a complete regression (CR) was defined as no palpable tumor at the end of the study.
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Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Niraparib was evaluated in mice using the MDA-MB-436 subcutaneous human breast tumor model. 7-9 week old female NOD SCID mice from Beijing Anikeeper Biotech Co. Ltd were injected subcutaneously with 10×106 MDA-MB-436 tumor cells. When tumors reached a volume of approximately 319 mm3, mice were randomized into groups of 5 and dosed daily (qd) for control, Niraparib (20 mg/kg) alone, Niraparib (50 mg/kg) alone or a combination group of Formula I (100 mg/kg) and Niraparib (20 mg/kg). Mice were dosed the relevant treatment via oral gavage once daily as highlighted above for 28 days.
Body weight and tumor volume was measured at least twice per week. Tumor volume was calculated as mean and standard error of the mean for each treatment group.
The data in
The drug-drug interaction (DDI) of the USP1 inhibitor Formula I co-crystal in combination with Olaparib was evaluated in mice by assessing plasma systemic exposure over time. 6-8 week old female NOD SCID mice from Beijing Anikeeper Biotech Co. Ltd were randomized into groups of 4 and dosed via oral gavage once daily for 5 days with Formula I (100 mg/kg) alone, Olaparib (50 mg/kg) alone, or with Formula I (100 mg/kg) and Olaparib (50 mg/kg) in combination.
For Olaparib (50 mg/kg) alone or in combination with for Formula I (100 mg/kg), blood samples were collected from each mouse at the following time points post day 1 and day 5 dose: pre-dose, 0.5 hr, 1 hr, 2 hr, 6 hr, 12 hr, 24 hr. For Formula I alone (100 mg/kg) or in combination with Olaparib (50 mg/kg), blood samples were collected from each mouse at the following time points post day 1 and day 5: pre-dose, 1 hr, 2 hr, 4 hr, 8 hr, 12 hr, and 24 hr.
The data in
In order to evaluate the toxicity and toxicokinetics of Formula I co-crystal, Formula I co-crystal was administered to male Sprague-Dawley rats for 10 days via oral gavage. Twenty-five 7-8 week old male rats (Rattus norvegicus) (5 mice/group) from (Envigo RMS, Inc., Indianapolis, Ind.) were administered vehicle or test article for ten days as either daily (SID) or twice daily (BID) oral doses as described in Table 4. Whole venous blood samples of approximately 0.5 mL were collected from a peripheral vein of the rats for determination of test article exposure. Samples were collected no Days 1 and 10: prior to administration (Day 10 only) and at 30 minutes, 1 hr, 2 hr, 4 hr, 8 hr, and 24 hrs after test article administration. All animals were euthanized for postmortem examinations approximately twenty-four hours post last dose.
Toxicokinetic analyses were conducted using Phoenix WinNonlin software (Version 8.1 or higher) using non-compartmental approach based on the route of administration.
To evaluate the toxicity and toxicokinetics of Formula I co-crystal, Formula I co-crystal was administered daily to male cynomolgus monkeys for 10 days. Fifteen 2-3 year old male cynomolgus monkeys (Macaca fascicularis) (3 animals/group) from Orient BioResource (Alice, Tex.) were administered vehicle or test articles via oral gavage for ten days as described in Table 5. In life, animals were observed for clinical signs of toxicity, changes in body weight and food consumption. Serial blood samples were collected for plasma concentration analysis to evaluate systemic test article exposure. All animals were euthanized for postmortem examinations approximately twenty-four hours post last dose (12 hours post last dose for BID arm).
Toxicokinetic analyses were conducted using Phoenix WinNonlin software (Version 8.1 or higher) using non-compartmental approach based on the route of administration.
A summary of the toxicity and toxicokinetic studies of Formula I co-crystal in Sprague-Dawley rats and cynomolgus monkeys in comparison to various PARP inhibitors is shown in Table 6. In comparison to various PARP inhibitors for which dose limiting toxicity is hematopoietic toxicity (myelosuppression and pancytopenia), the dose limiting toxicity for Formula I co-crystal is GI toxicity and thus, has non-overlapping dose limiting toxicity with PARP inhibitors. Table 6 further shows that hematopoietic toxicity is dose limiting for all approved PARP inhibitors. The clinical dose interruption, reduction, and discontinuation of PARP inhibitors are common due to patients experiencing adverse events when administered PARP inhibitors, so there is a need for a more tolerable and efficacious PARP inhibitor combination regimen. Thus, an opportunity exists for combination USP1 inhibitor and PARP inhibitor treatment, which could further enhance the efficacy of PARP inhibitors at reduced dose without overlapping toxicity.
Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using a variety of patient-derived xenograft models, including the triple negative breast cancer BRCA1 mutant, TP53 mutant, HRD high, and RAD51 high HBCx-8 model. 6-9 week old female athymic nude mice from Envigo were anesthetized, and a 20 mm3 tumor fragment was placed subcutaneously via incision in the flank. When tumors reached a tumor volume ranging from approximately 60 to 130 mm3, mice were randomized into groups of 3 mice and were assigned into the following groups: control (vehicle), Formula I (100 mg/kg), Olaparib (100 mg/kg), or Formula I (100 mg/kg) and Olaparib (100 mg/kg) combination. Compound was administered via oral gavage once daily for 42 days (days 0 to 41). Body weight and tumor volume were measured twice per week. Tolerability was assessed by calculating body weight changes as percent (%) from body weight on day of treatment start (day 0). Tumor volume was calculated as mean and standard error of the mean for each treatment group. A tumor regression (REG) was defined as a tumor with a smaller volume of the last day of the study as compared to the first day of dosing, and a complete regression (CR) was defined as no palpable tumor at the end of the study.
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Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using a variety of patient-derived xenograft models, including the triple negative breast cancer HBCx-17 model. 6-9 week old female athymic nude mice from Envigo were anesthetized, and a 20 mm3 tumor fragment was placed subcutaneously via incision in the flank. When tumors reached a tumor volume ranging from approximately 60 to 200 mm3, mice were randomized into groups of 8-10 mice and were assigned into the following groups: control (vehicle), Formula I (100 mg/kg), Formula I (300 mg/kg), Olaparib (50 mg/kg), Olaparib (100 mg/kg), Formula I (100 mg/kg) and Olaparib (50 mg/kg) combination, or Formula I (100 mg/kg) and Olaparib (100 mg/kg) combination. Compound was administered via oral gavage once daily for 43 days (day 0 to 42). Body weight and tumor volume were measured twice per week. Tolerability was assessed by calculating body weight changes as percent (%) from body weight on day of treatment start (day 0). Tumor volume was calculated as mean and standard error of the mean for each treatment group. A tumor regression (REG) was defined as a tumor with a smaller volume of the last day of the study as compared to the first day of dosing, and a complete regression (CR) was defined as no palpable tumor at the end of the study.
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Anti-tumor activity of the USP1 inhibitor, Formula I co-crystal in combination with Olaparib was evaluated in mice using a variety of patient-derived xenograft models, including the serous ovarian carcinoma model CTG-0703. 6-8 week old female athymic nude mice from Envigo were anesthetized, and a 60 mm3 tumor fragment was placed subcutaneously via incision in the flank. When tumors reached a tumor volume ranging from approximately 110 to 230 mm3, mice were randomized into groups of 3 mice and were assigned into the following groups: control (vehicle), Formula I (100 mg/kg), Formula I (300 mg/kg), Olaparib (50 mg/kg), Olaparib (100 mg/kg), Formula I (100 mg/kg) and Olaparib (50 mg/kg) combination, or Formula I (100 mg/kg) and Olaparib (100 mg/kg) combination. Compound was administered via oral gavage once daily for 60 days (day 0 to 59). Body weight and tumor volume were measured twice per week. Tolerability was assessed by calculating body weight changes as percent (%) from body weight on day of treatment start (day 0). Tumor volume was calculated as mean and standard error of the mean for each treatment group. A tumor regression (REG) was defined as a tumor with a smaller volume of the last day of the study as compared to the first day of dosing, and a complete regression (CR) was defined as no palpable tumor at the end of the study.
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Combination studies for three additional patient-derived ovarian carcinoma xenograft models, OV5308, OV5392, and OV0243, were carried out similarly to the above examples. No combination activity was observed in the OV5308, OV5392, and OV0243 models.
To perform CRISPR-Cas9 resistance screens, breast and ovarian cancer cell lines known to be sensitive to USP1 inhibition and/or PARP1 inhibitors were engineered to express Cas9 and were subsequently infected with lentivirus expressing guide RNAs targeting 1500 genes (20 sgRNAs per gene) involved in the DNA damage response and DNA repair. Infected cells were expanded for 10 days and split into different compound treatment arms: DMSO (negative control), 300 nM Formula I co-crystal, 300 nM Olaparib, and combination of 150 nM Formula I co-crystal plus 150 nM Olaparib. After 14 days of culture in the presence of drug, cells were harvested, genomic DNA was extracted, and Illumina Sequencing was used to determine guide representation. To determine the effect of a perturbation, the abundance of each sgRNA was compared to a reference sample, using both the plasmid library and the timepoint immediately prior to compound treatment initiation as references. For each guide in the library, the number of reads associated with that guide were counted, and the log-fold-change (logFC), defined as: logFC=log ((sample count+1)/(reference count+1)) was calculated. To ensure the magnitude of effect was comparable between experimental conditions, the scores associated with each guide were standardized by subtracting the median logFC for each sample from each guide, and dividing by the median absolute deviation, producing a Z-score for each guide. To aggregate guide-level scores to the gene-level, a per-gene “dropout score” was calculated for each gene targeted by the library by taking the median Z-score of all guides that target that gene. Differential dependencies, where CRISPR induced loss of gene function increases the cell's fitness in the presence of drug compared to DMSO treatment, were used to identify mechanism of drug resistance. For each gene a Fisher's Exact Test was used to test for an association between drug treatment and number of clones recovered, with false discoveries controlled using Benjamini Hochberg p-value adjustment. To assess screen quality, non-cutting neutral control guides were included, as well as positive control guides that target thousands of locations in the genome and robustly induce cell death. Control guides behaved as expected in screens of the breast cancer cell line MDA-MB-436, displaying a separation between positive and neutral control guides across all samples, and the majority of guides having no effect on fitness (
Having now fully described this invention, it will be understood by those of ordinary skill in the art that the same can be performed within a wide and equivalent range of conditions, formulations, and other parameters without affecting the scope of the invention or any embodiment thereof.
Other aspects of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the invention being indicated by the following claims.
All patents and publications cited herein are fully incorporated by reference herein in their entirety.
This application claims the benefit of U.S. Provisional Application Nos. 63/146,937, filed Feb. 8, 2021, 63/032,245, filed May 29, 2020, and 62/976,864, filed Feb. 14, 2020, each of which is hereby incorporated by reference in its entirety
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US2021/017912 | 2/12/2021 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63146937 | Feb 2021 | US | |
| 63032245 | May 2020 | US | |
| 62976864 | Feb 2020 | US |
