Patent Application
20070021606
The present invention relates to novel compounds that function as potentiators of glutamate receptors, methods for their preparation, pharmaceutical compositions containing them and their use in therapy.
The metabotropic glutamate receptors (mGluR) constitute a family of GTP-binding-protein (G-protein) coupled receptors that are activated by glutamate, and have important roles in synaptic activity in the central nervous system, including neural plasticity, neural development and neurodegeneration.
Activation of mGluRs in intact mammalian neurons elicits one or more of the following responses: activation of phospholipase C; increases in phosphoinositide (PI) hydrolysis; intracellular calcium release; activation of phospholipase D; activation or inhibition of adenyl cyclase; increases or decreases in the formation of cyclic adenosine monophosphate (cAMP); activation of guanylyl cyclase; increases in the formation of cyclic guanosine monophosphate (cGMP); activation of phospholipase A2; increases in arachidonic acid release; and increases or decreases in the activity of voltage- and ligand-gated ion channels (Schoepp et al., 1993, Trends Pharmacol. Sci., 14:13; Schoepp, 1994, Neurochem. Int., 24:439; Pin et al., 1995, Neuropharmacology 34:1; Bordi & Ugolini, 1999, Prog. Neurobiol. 59:55).
Eight mGluR subtypes have been identified, which are divided into three groups based upon primary sequence similarity, signal transduction linkages, and pharmacological profile. Group-I includes mGluR1 and mGluR5, which activate phospholipase C and the generation of an intracellular calcium signal. The Group-II (mGluR2 and mGluR3) and Group-III (mGluR4, mGluR6, mGluR7, and mGluR8) mGluRs mediate an inhibition of adenylyl cyclase activity and cyclic AMP levels. For a review, see Pin et al., 1999, Eur. J. Pharmacol., 375:277-294.
Members of the mGluR family of receptors are implicated in a number of normal processes in the mammalian CNS, and are important targets for compounds for the treatment of a variety of neurological and psychiatric disorders. Activation of mGluRs is required for induction of hippocampal long-term potentiation and cerebellar long-term depression (Bashir et al., 1993, Nature, 363:347; Bortolotto et al., 1994, Nature, 368:740; Aiba et al., 1994, Cell, 79365; Aiba et al., 1994, Cell, 79:377). A role for mGluR activation in nociception and analgesia also has been demonstrated (Meller et al., 1993, Neuroreport, 4: 879; Bordi & Ugolini, 1999, Brain Res., 871:223). In addition, mGluR activation has been suggested to play a modulatory role in a variety of other normal processes including synaptic transmission, neuronal development, apoptotic neuronal death, synaptic plasticity, spatial learning, olfactory memory, central control of cardiac activity, waking, motor control and control of the vestibulo-ocular reflex (Nakanishi, 1994, Neuron, 13:1031; Pin et al., 1995, Neuropharmacology, supra; Knopfel et al., 1995, J. Med. Chem., 38:1417).
Recent advances in the elucidation of the neurophysiological roles of mGluRs have established these receptors as promising drug targets in the therapy of acute and chronic neurological and psychiatric disorders and chronic and acute pain disorders. Because of the physiological and pathophysiological significance of the mGluRs, there is a need for new drugs and compounds that can modulate mGluR function.
The present invention is based upon the discovery of compounds that act as allosteric modulators of mGluR2.
Definitions
Unless specified otherwise within this specification, the nomenclature used in this specification generally follows the examples and rules stated in Nomenclature of Organic Chemistry, Sections A, B, C, D, E, F, and H, Pergamon Press, Oxford, 1979, which is incorporated by references herein for its exemplary chemical structure names and rules on naming chemical structures. Optionally, a name of a compound may be generated using a chemical naming program: ACD/ChemSketch, Version 5.09/September 2001, Advanced Chemistry Development, Inc., Toronto, Canada.
The term “Cm-n” or “Cm-n group” used alone or as a prefix, refers to any group having m to n carbon atoms, and having 0 to n multivalent heteroatoms selected from O, S and N, wherein m and n are 0 or positive integers, and n>m. For example, “C1-6” would refer to a chemical group having 1 to 6 carbon atoms, and having 0 to 6 multivalent heteroatoms selected from O, S and N.
The term “hydrocarbon” used alone or as a suffix or prefix, refers to any structure comprising only carbon and hydrogen atoms up to 14 carbon atoms.
The term “hydrocarbon radical” or “hydrocarbyl” used alone or as a suffix or prefix, refers to any structure as a result of removing one or more hydrogens from a hydrocarbon.
The term “alkyl” used alone or as a suffix or prefix, refers to monovalent straight or branched chain hydrocarbon radicals comprising 1 to about 12 carbon atoms. Unless otherwise specified, “alkyl” general includes both saturated alkyl and unsaturated alkyl.
The term “alkylene” used alone or as suffix or prefix, refers to divalent straight or branched chain hydrocarbon radicals comprising 1 to about 12 carbon atoms, which serves to links two structures together.
The term “alkenyl” used alone or as suffix or prefix, refers to a monovalent straight or branched chain hydrocarbon radical having at least one carbon-carbon double bond and comprising at least 2 up to about 12 carbon atoms.
The term “alkynyl” used alone or as suffix or prefix, refers to a monovalent straight or branched chain hydrocarbon radical having at least one carbon-carbon triple bond and comprising at least 2 up to about 12 carbon atoms.
The term “cycloalkyl,” used alone or as suffix or prefix, refers to a monovalent ring-containing hydrocarbon radical comprising at least 3 up to about 12 carbon atoms.
The term “cycloalkenyl” used alone or as suffix or prefix, refers to a monovalent ring-containing hydrocarbon radical having at least one carbon-carbon double bond and comprising at least 3 up to about 12 carbon atoms.
The term “cycloallcynyl” used alone or as suffix or prefix, refers to a monovalent ring-containing hydrocarbon radical having at least one carbon-carbon triple bond and comprising about 7 up to about 12 carbon atoms.
The term “aryl” used alone or as suffix or prefix, refers to a monovalent hydrocarbon radical having one or more polyunsaturated carbon rings having aromatic character, (e.g., 4n+2 delocalized electrons) and comprising 5 up to about 14 carbon atoms.
The term “arylene” used alone or as suffix or prefix, refers to a divalent hydrocarbon radical having one or more polyunsaturated carbon rings having aromatic character, (e.g., 4n+2 delocalized electrons) and comprising 5 up to about 14 carbon atoms, which serves to links two structures together.
The term “heterocycle” used alone or as a suffix or prefix, refers to a ring-containing structure or molecule having one or more multivalent heteroatoms, independently selected from N, O and S, as a part of the ring structure and including at least 3 and up to about 20 atoms in the ring(s). Heterocycle may be saturated or unsaturated, containing one or more double bonds, and heterocycle may contain more than one ring. When a heterocycle contains more than one ring, the rings may be fused or unfused. Fused rings generally refer to at least two rings share two atoms therebetween. Heterocycle may have aromatic character or may not have aromatic character.
The term “heteroalkyl” used alone or as a suffix or prefix, refers to a radical formed as a result of replacing one or more carbon atom of an alkyl with one or more heteroatoms selected from N, O and S.
The term “heteroaromatic” used alone or as a suffix or prefix, refers to a ring-containing structure or molecule having one or more multivalent heteroatoms, independently selected from N, O and S, as a part of the ring structure and including at least 3 and up to about 20 atoms in the ring(s), wherein the ring-containing structure or molecule has an aromatic character (e.g., 4n+2 delocalized electrons).
The term “heterocyclic group,” “heterocyclic moiety,” “heterocyclic,” or “heterocyclo” used alone or as a suffix or prefix, refers to a radical derived from a heterocycle by removing one or more hydrogens therefrom.
The term “heterocyclyl” used alone or as a suffix or prefix, refers a monovalent radical derived from a heterocycle by removing one hydrogen therefrom.
The term “heterocyclylene” used alone or as a suffix or prefix, refers to a divalent radical derived from a heterocycle by removing two hydrogens therefrom, which serves to links two structures together.
The term “heteroaryl” used alone or as a suffix or prefix, refers to a heterocyclyl having aromatic character.
The term “heterocylcoalkyl” used alone or as a suffix or prefix, refers to a heterocyclyl that does not have aromatic character.
The term “heteroarylene” used alone or as a suffix or prefix, refers to a heterocyclylene having aromatic character.
The term “heterocycloalkylene” used alone or as a suffix or prefix, refers to a heterocyclylene that does not have aromatic character.
The term “six-membered” used as prefix refers to a group having a ring that contains six ring atoms.
The term “five-membered” used as prefix refers to a group having a ring that contains five ring atoms.
A five-membered ring heteroaryl is a heteroaryl with a ring having five ring atoms wherein 1, 2 or 3 ring atoms are independently selected from N, O and S.
Exemplary five-membered ring heteroaryls are thienyl, furyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, pyrazolyl, isothiazolyl, isoxazolyl, 1,2,3-triazolyl, tetrazolyl, 1,2,3-thiadiazolyl, 1,2,3-oxadiazolyl, 1,2,4-triazolyl, 1,2,4-thiadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-triazolyl, 1,3,4-thiadiazolyl, and 1,3,4-oxadiazolyl.
A six-membered ring heteroaryl is a heteroaryl with a ring having six ring atoms wherein 1, 2 or 3 ring atoms are independently selected from N, O and S.
Exemplary six-membered ring heteroaryls are pyridyl, pyrazinyl, pyrimidinyl, triazinyl and pyridazinyl.
The term “substituted” used as a prefix refers to a structure, molecule or group, wherein one or more hydrogens are replaced with one or more C1-12hydrocarbon groups, or one or more chemical groups containing one or more heteroatoms selected from N, O, S, F, Cl, Br, I, and P. Exemplary chemical groups containing one or more heteroatoms include heterocyclyl, —NO2, —OR, —R′OR, —Cl, —Br, —I, —F, —CF3, —C(═O)R, —C(═O)OH, —NH2, —SH, —NHR, —NR2, —SR, —SO3H, —SO2R, —S(═O)R, —CN, —OH, —C(═O)OR, —C(═O)NR2, —NRC(═O)R, —NRC(═O)OR, —R′NR2, oxo (═O), imino (═NR), thio (═S), and oximino (═N—OR), wherein each “R” is hydrogen or a C1-12hydrocarbyl and “R′” is a C1-12hydrocarbyl. For example, substituted phenyl may refer to nitrophenyl, pyridylphenyl, methoxyphenyl, chlorophenyl, aminophenyl, etc., wherein the nitro, pyridyl, methoxy, chloro, and amino groups may replace any suitable hydrogen on the phenyl ring.
The term “substituted” used as a suffix of a first structure, molecule or group, followed by one or more names of chemical groups refers to a second structure, molecule or group, which is a result of replacing one or more hydrogens of the first structure, molecule or group with the one or more named chemical groups. For example, a “phenyl substituted by nitro” refers to nitrophenyl.
The term “optionally substituted” refers to groups, structures, or molecules that are substituted and to those that are not substituted.
Heterocycle includes, for example, monocyclic heterocycles such as: aziridine, oxirane, thiirane, azetidine, oxetane, thietane, pyrrolidine, pyrroline, imidazolidine, pyrazolidine, pyrazoline, dioxolane, sulfolane 2,3-dihydrofuran, 2,5-dihydrofuran tetrahydrofuran, thiophane, piperidine, 1,2,3,6-tetrahydro-pyridine, piperazine, morpholine, thiomorpholine, pyran, thiopyran, 2,3-dihydropyran, tetrahydropyran, 1,4-dihydropyridine, 1,4-dioxane, 1,3-dioxane, dioxane, homopiperidine, 2,3,4,7-tetrahydro-1H-azepine homopiperazine, 1,3-dioxepane, 4,7-dihydro-1,3-dioxepin, and hexamethylene oxide.
In addition, heterocycle includes aromatic heterocycles, for example, pyridine, pyrazine, pyrimidine, pyridazine, thiophene, furan, furazan, pyrrole, imidazole, thiazole, oxazole, pyrazole, isothiazole, isoxazole, 1,2,3-triazole, tetrazole, 1,2,3-thiadiazole, 1,2,3-oxadiazole, 1,2,4-triazole, 1,2,4-thiadiazole, 1,2,4-oxadiazole, 1,3,4-triazole, 1,3,4-thiadiazole, and 1,3,4-oxadiazole.
Additionally, heterocycle encompass polycyclic heterocycles, for example, indole, indoline, isoindoline, quinoline, tetrahydroquinoline, isoquinoline, tetrahydroisoquinoline, 1,4-benzodioxan, coumarin, dihydrocoumarin, benzofuran, 2,3-dihydrobenzofuran, isobenzofuran, chromene, chroman, isochroman, xanthene, phenoxathiin, thianthrene, indolizine, isoindole, indazole, purine, phthalazine, naphthyridine, quinoxaline, quinazoline, cinnoline, pteridine, phenanthridine, perimidine, phenanthroline, phenazine, phenothiazine, phenoxazine, 1,2-benzisoxazole, benzothiophene, benzoxazole, benzthiazole, benzimidazole, benztriazole, thioxanthine, carbazole, carboline, acridine, pyrolizidine, and quinolizidine.
In addition to the polycyclic heterocycles described above, heterocycle includes polycyclic heterocycles wherein the ring fusion between two or more rings includes more than one bond common to both rings and more than two atoms common to both rings. Examples of such bridged heterocycles include quinuclidine, diazabicyclo[2.2.1]heptane and 7-oxabicyclo[2.2.1]heptane.
Heterocyclyl includes, for example, monocyclic heterocyclyls, such as: aziridinyl, oxiranyl, thiiranyl, azetidinyl, oxetanyl, thietanyl, pyrrolidinyl, pyrrolinyl, imidazolidinyl, pyrazolidinyl, pyrazolinyl, dioxolanyl, sulfolanyl, 2,3-dihydrofuranyl, 2,5-dihydrofuranyl, tetrahydrofuranyl, thiophanyl, piperidinyl, 1,2,3,6-tetrahydro-pyridinyl, piperazinyl, morpholinyl, thiomorpholinyl, pyranyl, thiopyranyl, 2,3-dihydropyranyl, tetrahydropyranyl, 1,4-dihydropyridinyl, 1,4-dioxanyl, 1,3-dioxanyl, dioxanyl, homopiperidinyl, 2,3,4,7-tetrahydro-1H-azepinyl, homopiperazinyl, 1,3-dioxepanyl, 4,7-dihydro-1,3-dioxepinyl, and hexamethylene oxidyl.
In addition, heterocyclyl includes aromatic heterocyclyls or heteroaryl, for example, pyridinyl, pyrazinyl, pyrimidinyl, pyridazinyl, thienyl, furyl, furazanyl, pyrrolyl, imidazolyl, thiazolyl, oxazolyl, pyrazolyl, isothiazolyl, isoxazolyl, 1,2,3-triazolyl, tetrazolyl, 1,2,3-thiadiazolyl, 1,2,3-oxadiazolyl, 1,2,4-triazolyl, 1,2,4-thiadiazolyl, 1,2,4-oxadiazolyl, 1,3,4-triazolyl, 1,3,4-thiadiazolyl, and 1,3,4 oxadiazolyl.
Additionally, heterocyclyl encompasses polycyclic heterocyclyls (including both aromatic or non-aromatic), for example, indolyl, indolinyl, isoindolinyl, quinolinyl, tetrahydroquinolinyl, isoquinolinyl, tetrahydroisoquinolinyl, 1,4-benzodioxanyl, coumarinyl, dihydrocoumarinyl, benzofuranyl, 2,3-dihydrobenzofuranyl, isobenzofuranyl, chromenyl, chromanyl, isochromanyl, xanthenyl, phenoxathiinyl, thianthrenyl, indolizinyl, isoindolyl, indazolyl, purinyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, pteridinyl, phenanthridinyl, perimidinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, phenoxazinyl, 1,2-benzisoxazolyl, benzothiophenyl, benzoxazolyl, benzthiazolyl, benzimidazolyl, benztriazolyl, thioxanthinyl, carbazolyl, carbolinyl, acridinyl, pyrolizidinyl, and quinolizidinyl.
In addition to the polycyclic heterocyclyls described above, heterocyclyl includes polycyclic heterocyclyls wherein the ring fusion between two or more rings includes more than one bond common to both rings and more than two atoms common to both rings. Examples of such bridged heterocycles include quinuclidinyl, diazabicyclo[2.2.1]heptyl; and 7-oxabicyclo[2.2.1]heptyl.
The term “alkoxy” used alone or as a suffix or prefix, refers to radicals of the general formula —O—R, wherein R is selected from a hydrocarbon radical. Exemplary alkoxy includes methoxy, ethoxy, propoxy, isopropoxy, butoxy, t-butoxy, isobutoxy, cyclopropylmethoxy, allyloxy, and propargyloxy.
The term “amine” or “amino” used alone or as a suffix or prefix, refers to radicals of the general formula —NRR′, wherein R and R′ are independently selected from hydrogen or a hydrocarbon radical.
“Acyl” used alone, as a prefix or suffix, means —C(═O)—R, wherein R is an optionally substituted hydrocarbyl, hydrogen, amino or alkoxy. Acyl groups include, for example, acetyl, propionyl, benzoyl, phenyl acetyl, carboethoxy, and dimethylcarbamoyl.
“Halogen” includes fluorine, chlorine, bromine and iodine.
“Halogenated,” used as a prefix of a group, means one or more hydrogens on the group is replaced with one or more halogens.
“RT” or “rt” means room temperature.
A first ring group being “fused” with a second ring group means the first ring and the second ring share at least two atoms therebetween.
“Link,” “linked,” or “linking,” unless otherwise specified, means covalently linked or bonded.
In one aspect, the present invention provides a compound of formula I, a pharmaceutically acceptable salt thereof, diastereomers, enantiomers, or mixtures thereof:
wherein
R is H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted alkylamino, optionally substituted cycloalkylamino, optionally substituted arylamino, optionally substituted aminoaryl, optionally substituted heteroarylamino, optionally substituted aminoheteroaryl, optionally substituted alkoxy, optionally substituted aryloxy, heteroaryl substituted with an optionally substituted heteroaryl group, or
R1 and R2 are each, independently, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted alkylamino, or a divalent C1-8 group, or R1, R2, and the N to which they are attached, in combination, form a heterocycle which is optionally substituted with one or more substituents;
W is C═O, CH2, C═NR7, C═S, or optionally substituted alkyl;
Y is CH2, NR8, O, S, SO, or SO2; or
W and Y together are —CH═CH—;
V is C or S;
m is 0 or 1;
Z is, at each position, independently, halogen, cyano, optionally substituted alkyl, optionally substituted alkoxy, or optionally substituted alkylamino;
n is 0, 1, 2,3, or 4; and
R3, R4, R5, R6, R7, and R8 are each, independently, H or optionally substituted alkyl.
In some embodiments, the compounds of the present invention are those of formula I, wherein R is H, optionally substituted C1-12 alkyl, optionally substituted C3-12 cycloalkyl, optionally substituted C2-12 heterocycloalkyl, optionally substituted C6-12 aryl, optionally substituted C2-12 heteroaryl, optionally substituted C1-6 alkylamino, optionally substituted C3-12 cycloalkylamino, phenylamino, aminophenyl, optionally substituted pyridiylamino, optionally substituted aminopyridyl, optionally substituted C1-6 alkoxy, optionally substituted aryloxy, or
R1 and R2 are each, independently, optionally substituted C1-6 alkyl, optionally substituted C3-6 cycloalkyl, optionally substituted C3-6 heterocycloalkyl, optionally substituted C1-6 alkylamino, or a C1-8 alkylene group, or R1, R2, and the N to which they are attached, in combination, form a heterocycle which is optionally substituted with one or more substituents;
W is C═O or CH2;
Y is CH2, NR8, or O;
V is C;
m is 1; and
Z is, at each position, independently, halogen, optionally substituted C1-12 alkyl, optionally substituted C1-6 alkoxy, or optionally substituted C1-6 alkylamino.
In some embodiments, the compounds of the present invention are those of formula I, wherein R is phenyl, dimethoxyphenyl, methoxyphenyl, chlorophenyl, pyridyl, fluorophenyl, ethyl, naphthyl, t-butyl, optionally substituted pyrrolidinyl, N-methylphenylamino, optionally substituted piperidinyl, cyclohexyl, quinolinyl,
R1 and R2 are each independently C1-6 alkyl, or R1, R2, and the N to which they are attached, in combination, are
W is CH2 or C═O;
Y is CH2, NH, NCH3, or O;
V is C;
m is 1;
Z is, at each position, independently, Cl, F, methoxy, or methyl; and
R3 and R4 are each, independently, H, methyl, or ethyl.
In some embodiments, the compounds of the present invention are those of formula I, wherein R1, R2, and the N to which they are attached, in combination, are
In some embodiments, the compounds of the present invention are those of formula I, wherein Z is 2-chloro.
In one embodiment of the invention there are provided compounds of formula IV
wherein
R is H, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted alkylamino, optionally substituted cycloalkylamino, optionally substituted arylamino, optionally substituted aminoaryl, optionally substituted heteroarylamino, optionally substituted aminoheteroaryl, optionally substituted alkoxy, optionally substituted aryloxy, heteroaryl substituted with an optionally substituted heteroaryl group, or
R1 and R2 are each, independently, optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted alkylamino, or a divalent C1-8 group, or R1, R2, and the N to which they are attached, in combination, form a heterocycle which is optionally substituted with one or more substituents;
Y is CH2, NR8, O, S, SO, or SO2;
Z is, at each position, independently, halogen, cyano, optionally substituted alkyl, optionally substituted alkoxy, or optionally substituted alkylamino;
n is 0, 1, 2, 3, or 4; and
R5, R6, R8 and are each, independently, H or optionally substituted alkyl.
In another embodiment of the invention there are provided compounds of formula VI
wherein
the variables R, Z, n, R5, and R6 are as described in relation to formula IV.
In still another embodiment of the invention there are provided compounds of formula
wherein
the variables R, Z, n, R5, and R6 are as described in relation to formula IV.
In yet another embodiment of the invention there are provided compounds of formula
wherein
the variables R, Z, n, R5, and R6 are as described in relation to formula IV.
In yet another embodiment of the invention there are provided compounds of formula
wherein
the variables R, Z, n, R5, and R6 are as described in relation to formula IV.
In yet another embodiment of the invention there are provided compounds of formula XX
wherein
the variables R, Z, n, R5, and R6 are as described in relation to formula IV.
In a further aspect, the present invention provides a process for preparing a compound of formula I.
In one embodiment, the invention provides a process for preparing a compound of formula IV, as shown in Scheme 1.
Appropriately substituted benzoic acids I were reacted with chlorosulphonic acid at 140° C. to provide sulphonyl chlorides II. These sulphonyl chlorides were reacted with various amines (i.e., R1R2NH) in a solvent such as THF or benzene to prepare sulphonamides III. The acid function in III can be alkylated with an alpha-bromo ketone using a base such as potassium carbonate. Alternatively, the acid can be converted into its acid chloride using typical methods, and allowed to react with nucleophiles HYCH2COR, where Y is O or NH, for example.
In another embodiment, the invention provides a process for preparing a compound of formula VI by the route shown in Scheme 2.
Benzoic acids III were reduced to benzyl alcohols V by first converting them to mixed anhydrides using isobutyl chloroformate, then allowing this intermediate to react with sodium borohydride. The alcohols obtained were alkylated to ethers VI using typical techniques.
In still a further embodiment, the invention provides a process for preparing compounds of formula IX as shown in Scheme 3.
Benzyl alcohol V was alkylated with t-butyl bromoacetate using standard conditions to provide t-butyl ester VII. The t-Butyl ester VII was deprotected with formic acid to yield the acid VIII. The acid VIII was then converted to amide 1× using standard amide bond forming conditions.
In yet a further embodiment, the invention provides a process for preparing compounds of formula XI by the route shown in Scheme 4.
Benzyl alcohol V was alkylated with 2-chloro-N-methoxy-N-methylacetamide using standard conditions to provide amide X. Reacting X with various organometallic reagents (e.g., Grignard or organolithium reagents) yielded ketones XI.
In still another embodiment, the invention provides a process for preparing compounds of formula XV as described in Scheme 5.
Benzoic acid III was converted to its acid chloride, then allowed to react with the Grignard reagent derived from 1-bromo-3,3-dimethoxypropane to yield the acetal XII. The protecting group was removed under acidic conditions to provide aldehyde XIII. The aldehyde was reacted with various organometallic reagents (e.g., Grignard, organolithium) to provide the secondary alcohol XIV. This alcohol was oxidized to the ketone XV using standard oxidizing reagents (e.g., PCC).
In yet another embodiment the invention describes a process for preparing compounds of formula XX by the route described in Scheme 6.
Ketoaldehyde XIII was reduced to the diol XVI using typical reducing agents (e.g., sodium borohydride). The benzylic alcohol in XVI was further reduced with InCl3 and Ph2SiHCl. Some alkene was formed via elimination in this reaction; it was hydrogenated to provide the pure saturated alcohol XVII. This alcohol was oxidized to the aldehyde XVIII using standard oxidizing reagents (e.g., PCC). The aldehyde was allowed to react with various organometallic reagents (e.g., Grignard or organolithium) to provide the secondary alcohol XIX. The final ketone XX was obtained by standard oxidation of XIX with PCC, for example.
Specific embodiments of the present invention include:
It will be understood by those of skill in the art that when compounds of the present invention contain one or more chiral centers, the compounds of the invention may exist in, and be isolated as, enantiomeric or diastereomeric forms, or as a racemic mixture. The present invention includes any possible enantiomers, diastereomers, racemates or mixtures thereof, of a compound of formula I, IV, VI, IX, XI, XV, or XX. The optically active forms of the compound of the invention may be prepared, for example, by chiral chromatographic separation of a racemate, by synthesis from optically active starting materials or by asymmetric synthesis based on the procedures described thereafter.
It will also be appreciated by those of skill in the art that certain compounds of the present invention may exist as geometrical isomers, for example E and Z isomers of alkenes. The present invention includes any geometrical isomer of a compound of formula I, IV, VI, IX, XI, XV, or XX. It will further be understood that the present invention encompasses tautomers of the compounds of formula I, IV, VI, IX, XI, XV, or XX.
It will also be understood by those of skill in the art that certain compounds of the present invention may exist in solvated, for example hydrated, as well as unsolvated forms. It will further be understood that the present invention encompasses all such solvated forms of the compounds of formula I, IV, VI, IX, XI, XV, or XX.
Within the scope of the invention are also salts of the compounds of formula I, IV, VI, IX, XI, XV, or XX. Generally, pharmaceutically acceptable salts of compounds of the present invention are obtained using standard procedures well known in the art, for example, by reacting a sufficiently basic compound, for example an alkyl amine with a suitable acid, for example, HCl or acetic acid, to afford a physiologically acceptable anion. It is also possible to make a corresponding alkali metal (such as sodium, potassium, or lithium) or an alkaline earth metal (such as a calcium) salt by treating a compound of the present invention having a suitably acidic proton, such as a carboxylic acid or a phenol with one equivalent of an alkali metal or alkaline earth metal hydroxide or alkoxide (such as the ethoxide or methoxide), or a suitably basic organic amine (such as choline or meglumine) in an aqueous medium, followed by conventional purification techniques.
In one embodiment of the present invention, the compound of formula I, IV, VI, IX, XI, XV, or XX may be converted to a pharmaceutically acceptable salt or solvate thereof, particularly, an acid addition salt such as a hydrochloride, hydrobromide, phosphate, acetate, fumarate, maleate, tartrate, citrate, methanesulphonate orp-toluenesulphonate.
We have discovered that the compounds of the present invention have activity as pharmaceuticals, in particular as modulators of metabotropic glutamate receptors. More particularly, the compounds of the present invention exhibit activity as potentiators of the mGluR2 receptor, and are useful in therapy, in particular for the treatment of neurological and psychiatric disorders associated with glutamate dysfunction.
Compounds of the present invention are useful in treating neurological and psychiatric disorders including, but are not limited to, neurological and psychiatric disorders such as cerebral deficit subsequent to cardiac bypass surgery and grafting, stroke, cerebral ischemia, spinal cord trauma, head trauma, perinatal hypoxia, cardiac arrest, hypoglycemic neuronal damage, dementia (including AIDS-induced dementia), Alzheimer's disease, Huntington's Chorea, amyotrophic lateral sclerosis, ocular damage, retinopathy, cognitive disorders, idiopathic and drug-induced Parkinson's disease, muscular spasms and disorders associated with muscular spasticity including tremors, epilepsy, convulsions, migraine (including migraine headache), urinary incontinence, substance tolerance, substance withdrawal (including, substances such as opiates, nicotine, tobacco products, alcohol, benzodiazepines, cocaine, sedatives, hypnotics, etc.), psychosis, schizophrenia, anxiety (including generalized anxiety disorder, panic disorder, and obsessive compulsive disorder, and post-traumatic stress disorder (PTSD)), mood disorders (including depression, mania, bipolar disorders), circadian rhythm disorders (including jet lag and shift work), trigeminal neuralgia, hearing loss, tinnitus, macular degeneration of the eye, emesis, brain edema, pain (including acute and chronic pain states, severe pain, intractable pain, neuropathic pain, inflammatory pain, and post-traumatic pain), tardive dyskinesia, sleep disorders (including narcolepsy), attention deficit/hyperactivity disorder, and conduct disorder.
Also within the scope of the invention is the use of any of the compounds according to formula I, IV, VI, IX, XI, XV, or XX, or a pharmaceutically acceptable-salt or solvate thereof, for the manufacture of a medicament for the treatment of any of the conditions discussed above.
A further aspect of the invention is a method for the treatment of a subject suffering from any of the conditions discussed above, whereby an effective amount of a compound according to formula I, IV, VI, IX, XI, XV, or XX, or a pharmaceutically acceptable salt or solvate thereof, is administered to a patient in need of such treatment.
Thus, the invention provides a compound of formula I, IV, VI, IX, XI, XV, or XX, or pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined for use in therapy.
In a further aspect, the present invention provides the use of a compound of formula I, IV, VI, IX, XI, XV, or XX, or a pharmaceutically acceptable salt or solvate thereof, as hereinbefore defined in the manufacture of a medicament for use in therapy.
In the context of the present specification, the term “therapy” also includes “prophylaxis” unless there are specific indications to the contrary. The term “therapeutic” and “therapeutically” should be construed accordingly. The term “therapy” within the context of the present invention further encompasses to administer an effective amount of a compound of the present invention, to mitigate either a pre-existing disease state, acute or chronic, or a recurring condition. This definition also encompasses prophylactic therapies for prevention of recurring conditions and continued therapy for chronic disorders.
The compounds of the present invention are useful in therapy, especially for the therapy of neurological and psychiatric disorders and conditions associated with glutamate dysfunction.
In use for therapy in a warm-blooded animal such as a human, the compounds of the present invention may be administered in the form of a conventional pharmaceutical composition by any route including orally, intramuscularly, subcutaneously, topically, intranasally, intraperitoneally, intrathoracially, intravenously, epidurafly, intrathecally, intracerebroventricularly and by injection into the joints.
In some embodiments of the invention, the route of administration is oral, intravenous, or intramuscular.
The dosage will depend on the route of administration, the severity of the disease, age and weight of the patient and other factors normally considered by the attending physician, when determining the individual regimen and dosage level at the most appropriate for a particular patient.
For preparing pharmaceutical compositions from the compounds of the present invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include, but are not limited to, powders, tablets, dispersible granules, capsules, cachets, and suppositories.
A solid carrier can be one or more substances, which may also act as diluents, flavoring agents, solubilizers, lubricants, suspending agents, binders, or table disintegrating agents. A solid carrier can also be an encapsulating material.
In powders, the carrier is a finely divided solid, which is in a mixture with the finely divided compound of the invention, or the active component. In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.
For preparing suppository compositions, a low-melting wax such as a mixture of fatty acid glycerides and cocoa butter is first melted and the active ingredient is dispersed therein by, for example, stirring. The molten homogeneous mixture is then poured into convenient sized moulds and allowed to cool and solidify.
Suitable carriers include, but are not limited to, magnesium carbonate, magnesium stearate, talc, lactose, sugar, pectin, dextrin, starch, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, low-melting wax, cocoa butter, and the like.
The term composition is also intended to include the formulation of the active component with encapsulating material as a carrier providing a capsule in which the active component (with or without other carriers) is surrounded by a carrier which is thus in association with it. Similarly, cachets are included.
Tablets, powders, cachets, and capsules can be used as solid dosage forms suitable for oral administration.
Liquid form compositions include solutions, suspensions, and emulsions. For example, sterile water or water propylene glycol solutions of the active compounds may be liquid preparations suitable for parenteral administration. Liquid compositions can also be formulated in solution in aqueous polyethylene glycol solution.
Aqueous solutions for oral administration can be prepared by dissolving the active component in water and adding suitable colorants, flavoring agents, stabilizers, and thickening agents as desired. Aqueous suspensions for oral use can be made by dispersing the finely divided active component in water together with a viscous material such as natural synthetic gums, resins, methyl cellulose, sodium carboxymethyl cellulose, and other suspending agents known to the pharmaceutical formulation art.
Depending on the mode of administration, the pharmaceutical composition will include from about 0.05% w (percent by weight) to about 99% w, more particularly, from about 0.10% w to 50% w, of the compound of the invention, all percentages by weight being based on total composition.
A therapeutically effective amount for the practice of the present invention can be determined, by the use of known criteria including the age, weight and response of the individual patient, and interpreted within the context of the disease which is being treated or which is being prevented, by one of ordinary skill in the art.
Within the scope of the invention is the use of any compound of formula I, IV, VI, IX, XI, XV, or XX as defined above for the manufacture of a medicament.
Also within the scope of the invention is the use of any compound of formula I, IV, VI, IX, XI, XV, or XX for the manufacture of a medicament for the therapy of neurological and psychiatric disorders including, but are not limited to, acute neurological and psychiatric disorders such as cerebral deficit subsequent to cardiac bypass surgery and grafting, stroke, cerebral ischemia, spinal cord trauma, head trauma, perinatal hypoxia, cardiac arrest, hypoglycemic neuronal damage, dementia (including AIDS-induced dementia), Alzheimer's disease, Huntington's Chorea, amyotrophic lateral sclerosis, ocular damage, retinopathy, cognitive disorders, idiopathic and drug-induced Parkinson's disease, muscular spasms and disorders associated with muscular spasticity including tremors, epilepsy, convulsions, migraine (including migraine headache), urinary incontinence, substance tolerance, substance withdrawal (including, substances such as opiates, nicotine, tobacco products, alcohol, benzodiazepines, cocaine, sedatives, hypnotics, etc.), psychosis, schizophrenia, anxiety (including generalized anxiety disorder, panic disorder, and obsessive compulsive disorder), mood disorders (including depression, mania, bipolar disorders), trigeminal neuralgia, hearing loss, tinnitus, macular degeneration of the eye, emesis, brain edema, pain (including acute and chronic pain states, sever pain, intractable pain, neuropathic pain, and post-traumatic pain), tardive dyslinesia, sleep disorders (including narcolepsy), attention deficit/hyperactivity disorder, and conduct disorder.
Additionally provided is the use of any compound according to formula I, IV, VI, IX, XI, XV, or XX for the manufacture of a medicament for the therapy of neurological and psychiatric disorders.
A further aspect of the invention is a method for therapy of a subject suffering from any of the conditions discussed above, whereby an effective amount of a compound according to formula I, IV, VI, IX, XI, XV, or XX, is administered to a patient in need of such therapy.
Additionally, there is provided a pharmaceutical composition comprising a compound of formula I, IV, VI, IX, XI, XV, or XX, or a pharmaceutically acceptable salt or solvate thereof, in association with a pharmaceutically acceptable carrier or excipient.
Particularly, there is provided a pharmaceutical composition comprising a compound of formula I, IV, VI, IX, XI, XV, or XX, or a pharmaceutically acceptable salt or solvate thereof, in association with a pharmaceutically acceptable carrier or excipient for therapy, more particularly for therapy of neurological and psychiatric disorders.
In another aspect of the present invention, there is provided a pharmaceutical composition comprising a compound of formula I, IV, VI, Ix, XI, XV, or XX, or a pharmaceutically acceptable salt or solvate thereof, in association with a pharmaceutically acceptable carrier or excipient for use in any of the conditions discussed above.
The compounds described herein may be provided or delivered in a form suitable for oral use, for example, in a tablet, lozenge, hard and soft capsule, aqueous solution, oily solution, emulsion, and suspension. The compounds of the present invention can be provided for topical administration, for example, as a cream, ointment, gel, spray, or aqueous solution, oily solution, emulsion or suspension. The compounds described herein can be provided in a form suitable for nasal administration, for example, as a nasal spray, nasal drops, or dry powder. The compounds can be administered to the vagina or rectum in the form of a suppository. The compounds described herein can be administered parentally, for example, by intravenous, intravesicular, subcutaneous, or intramuscular injection or infusion. The compounds can be administered by insufflation (for example as a finely divided powder). The compounds may also be administered transdermally or sublingually.
The compositions of the present invention can accordingly be obtained by conventional procedures using conventional pharmaceutical excipients, well known in the art. Thus, compositions intended for oral use may contain, for example, one or more coloring, sweetening, flavoring and/or preservative agents.
The amount of active ingredient that is combined with one or more excipients to produce a single dosage form will necessarily vary depending upon the host treated and the particular route of administration. The size of the dose for therapeutic or prophylactic purposes of a compound of formula I, IV, VI, IX, XI, XV, or XX will naturally vary according to the nature and severity of the conditions, the age and sex of the animal or patient and the route of administration, according to well known principles of medicine.
In addition to their use in therapeutic medicine, the compounds of formula I, IV, VI, IX, XI, XV, or XX, or salts thereof, are also useful as pharmacological tools in the development and standardisation of in vitro and in vivo test systems for the evaluation of the effects of inhibitors of mGluR-related activity in laboratory animals such as, for example, cats, dogs, rabbits, monkeys, rats and mice, as part of the search for new therapeutics agents.
We have discovered that the compounds of the present invention are active as modulators of mGluR2 function.
Generally the compounds were active in the following assays at concentrations (or with EC50 values) less than 10 μM.
The pharmacological properties of the compounds of the invention can be analyzed using standard assays for functional activity. Examples of glutamate receptor assays are well known in the art as described in, for example, Aramori et al., 1992, Neuron, 8:757; Tanabe et al., 1992, Neuron, 8:169; Miller et al., 1995, J. Neuroscience, 15:6103; Balazs, et al., 1997, J. Neurochemistry, 1997,69:151. The methodology described in these publications is incorporated herein by reference. Conveniently, the compounds of the invention can be studied by means of an assay that measures the mobilization of intracellular calcium, [Ca2+]i in cells expressing mGluR2.
Fluorometric Imaging Plate Reader (FLIPR) analysis was used to detect allosteric activators of mGluR2 via calcium mobilization. A clonal HEK 293 cell line expressing a chimeric mGluR2/CaR construct comprising the extracellular and transmembrane domains of human mGluR2 and the intracellular domain of the human calcium receptor, fused to the promiscuous chimeric protein Gαqi5 was used. Activation of this construct by agonists or allosteric activators resulted in stimulation of the PLC pathway and the subsequent mobilization of intracellular Ca2+ which was measured via FLIPR analysis. At 24-hours prior to analysis, the cells were trypsinized and plated in DMEM at 100,000 cells/well in black sided, clear-bottom, collagen I coated, 96-well plates. The plates were incubated under 5% CO2 at 37° C. overnight. Cells were loaded with 6 μM fluo-3 acetoxymethylester (Molecular Probes, Eugene Oreg.) for 60 minutes at room temperature. All assays were performed in a buffer containing 126 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, 20 mM Hepes, 0.06 μM DCG-IV (a Group II mGluR selective agonist), supplemented with 1.0 mg/ml D-glucose and 1.0 mg/ml BSA fraction IV (pH 7.4).
FLIPR experiments were done using a laser setting of 0.8 W and a 0.4 second CCD camera shutter speed. Extracellular fluo-3 was washed off and cells were maintained in 160 μL of buffer and placed in the FLIPR. An addition of test compound (0.0 μM to 30 μM in duplicate) was made after 10 seconds of baseline fluorescent readings were recorded on FLIPR. Fluorescent signals were then recorded for an additional 75 seconds at which point a second addition of DCG-IV (0.2 μM) was made and fluorescent signals were recorded for an additional 65 seconds. Fluorescent signals were measured as the peak height of the response within the sample period. Data was analyzed using Assay Explorer, and EC50 and Emax values (relative to maximum DCG-IV effect) were calculated using a four parameter logistic equation.
A [35S]-GTPγS binding assay was used to functionally assay mGluR2 receptor activation. The allosteric activator activity of compounds at the human mGluR2 receptor ere measured using a [35S]-GTPγS binding assay with membranes prepared from CHO cells which stably express the human mGluR2. The assay is based upon the principle that agonists bind to G-protein coupled receptors to stimulate GDP-GTP exchange at the G-protein. Since [35S]-GTPγS is a non-hydrolyzable GTP analog, it can be used to provide an index of GDP-GTP exchange and, thus, receptor activation. The GTPγS binding assay therefore provides a quantitative measure of receptor activation.
Membranes were prepared from CHO cells stably transfected with human mGluR2. Membranes (30 μg protein) were incubated with test compound (3 nM to 300 μM) for 15 minutes at room temperature prior to the addition of 1 μM glutamate, and incubated for 30 min at 30° C. in 500 μl assay buffer (20 mM HEPES, 100 mM NaCl, 100 mM MgCl2), containing 30 μM GDP and 0.1 nM [35S]-GTPγS (1250 Ci/mmol). Reactions were carried out in triplicate in 2 ml polypropylene 96-well plates. Reactions were terminated by vacuum filtration using a Packard 96-well harvester and Unifilter-96, GF/B filter microplates. The filter plates were washed 4×1.5 ml with ice-cold wash buffer (10 mM sodium phosphate buffer, pH 7.4). The filter plates were dried and 35 μl of scintillation fluid (Microscint 20) was added to each well. The amount of radioactivity bound was determined by counting plates on the Packard TopCount. Data was analyzed using GraphPad Prism, and EC50 and Emax values (relative to the maximum glutamate effect) were calculated using non-linear regression.
The invention is further illustrated by way of the following examples, which are intended to elaborate several embodiments of the invention. These examples are not intended to, nor are they to be construed to, limit the scope of the invention. It will be clear that the invention may be practiced otherwise than as particularly described herein. Numerous modifications and variations of the present invention are possible in view of the teachings herein and, therefore, are within the scope of the invention.
General Synthesis of Chlorosulfonylbenzoic Acids
To chlorosulfonic acid (as solvent) cooled in an ice-bath was slowly added an appropriate benzoic acid. After the addition of the acid, the resulting mixture was heated to 140° C. for 3 h. The mixture was then cooled to room temperature and added dropwise to stirred ice-water (150 mL). The resulting precipitate was filtered and the solid residue obtained was used directly in the next step.
The following chlorosulfonylbenzoic acids were synthesized using the above procedure.
To a mixture of an appropriate chlorosulfonylbenzoic acid in benzene or THF was added the alkylamine. The mixture was then stirred at room temperature for 2 h after which the solvent was removed in vacuo and the residue was diluted with 1N HCl. The reaction mixture was then extracted with ethyl acetate or dichloromethane, and the organic extract was washed with brine, dried over MgSO4 (anhydrous). The organic solvent was removed in vacuo and the product was triturated with 10% ethyl acetate/hexane.
The following alkylaminosulfonylbenzoic acids were synthesized using the above procedure.
To a stirred solution of 2-hydroxy-5-[piperidinosulfonyl]benzoic acid (1.0 g, 3.5 mmol) in DMF (10 mL) was added NaOH (500 mg, 12.5 mmol) and the mixture was heated to 60° C. for 0.5 h. To the resulting white solid suspension was added MeI (1.5 g, 10.5 mmol) and stirring was continued overnight. The mixture was then diluted with water and extracted with ethyl acetate. The organic layer was washed with brine and dried over MgSO4 (anhydrous). The organic solvent was then removed in vacuo to afford the product as a yellow oil (11.0 g, 91% yield). The ester was then dissolved in a THF/MeOH/H2O (10 mL/5 mL/2 mL) mixture, LiOH (270 mg, 6.4 mmol) was added and the mixture was heated to 90° C. for 0.5 h. Upon cooling, the mixture was concentrated in vacuo and the pH of the resulting solution was adjusted to pH 3. The off-white precipitate obtained was filtered, collected and dried giving 842 mg or 88% yield.
To a solution of a benzoic acid in anhydrous CH2Cl2 was added triethylamine (1.5 eq.) and isobutyl chloroformate (1.5 eq.) at 0° C. After 2 hours the reaction mixture was concentrated. The residue was triturated with anhydrous THF and filtered. The filtrate was chilled in an ice bath and a solution of NaBH4 (5 eq.) in water was added dropwise. The reaction was slowly allowed to warm to room temperature. After 16 hours the reaction was quenched with 6M HCl and basified with saturated NaHCO3. This was partitioned between ether and saturated NaHCO3. The organic phase was washed with brine, dried (NasSO4), filtered, and concentrated. The (alkylaminosulphonyl)phenyhnethanols were isolated by column chromatography.
The following (allcylaminosulfonyl)phenyhnethanols were synthesized using the above procedure.
To a solution of (2-chloro-5-(piperidine-1-sulphonyl)phenyl)methanol (103 mg, 0.355 mmol) dissolved in anhydrous dichloromethane (3 mL) was added carbontetrabromide (130 mg, 0.391 mmol). The reaction mixture was cooled to 0° C. and triphenylphosphine (103 mg, 0.391 mmol) was added. The reaction was warmed to room temperature over 2 hours. It was poured into brine and diluted with dichloromethane. The organic was dried over sodium sulphate and the solvent was removed in vacuo. Eluting through a solid phase extraction tube (5 g) with 20% ethyl acetate/hexanes afforded 1-(3-bromomethyl-4-chloro-benzenesulphonyl)-piperidine (135 mg, 108%) as a white solid.
1H-NMR (CDCl3) δ: 7.84 (dd, 1H) 7.62 (dd, 1H), 7.53-7.57 (m, 1H) 4.73 (s, 1H), 4.60 (s, 1H), 3.00 (s, 4H), 1.2-1.69 (m, 4H), 1.44-1.48 (m, 2H).
Method A
To a solution of an appropriate alkylaminosulfonylbenzoic acid (1 eqv.) in DMF was added K2CO3 (2 eqv) and the mixture was heated with a heat gun for 1 minute. Bromoacetophenone (1 eqv) was then added and the mixture was heated for a further minute. The reaction was allowed to cool to room temperature and diluted with ethyl acetate and washed with brine, dried over MgSO4 (anhydrous). The organic solvent was removed in vacuo and the product was triturated with hexane to afford the product after filtration.
1H-NMR (CDCl3) δ: 8.70 (s, 1H), 7.95 (d, 2H), 7.67 (s, 1H), 7.63 (t, 1H), 7.52 (t, 2H), 5.60 (s, 2H), 3.30 (t, 4H), 1.60 (m, 6H).
1H-NMR (CDCl3) δ: 8.73 (s, 1H), 7.95 (d, 2H), 7.68 (s, 1H), 7.63 (t, 1H), 7.52 (t, 2H), 5.61 (s, 2H), 3.45 (t, 4H), 1.94 (t, 4H).
1H-NMR (CDCl3) δ: 8.69 (s, 1H), 7.95 (d, 2H), 7.69 (s, 1H), 7.64 (t, 1H), 7.52 (t, 2H), 5.61 (s, 2H), 4.10 (t, 4H), 2.26 (m, 2H).
1H-NMR (CDCl3) δ: 8.71 (s, 1H), 7.95 (d, 2H), 7.67 (s, 1H), 7.63 (t, 1H), 7.52 (t, 2H), 5.60 (s, 2H), 3.60 (t, 4H), 2.70 (t, 4H).
1H-NMR (CDCl3) δ: 8.72 (s, 1H), 7.95 (d, 2H), 7.67 (s, 1H), 7.65 (t, 1H), 7.52 (t, 2H), 5.61 (s, 2H), 3.40 (q, 4H), 1.10 (t, 6H).
1H-NMR (CDCl3) δ: 8.70 (s, 1H), 7.95 (d, 2H), 7.67 (s, 1H), 7.64 (t, 1H), 7.52 (t, 2H), 5.62 (s, 2H), 3.42 (t, 4H), 1.80 (m, 4H), 1.68 (m, 4H).
1H-NMR (CDCl3) δ: 8.36 (s, 1H), 7.96 (d, 2H), 7.89 (dd, 1H), 7.63 (t, 1H), 7.52 (t, 2H), 7.10 (d, 1H), 5.60 (s, 2H), 3.99 (s, 3H), 3.10 (t, 4H), 1.65 (m, 4H), 1.43 (m, 2H).
1H-NMR (CDCl3) δ: 8.40 (s, 1H), 7.96 (d, 2H), 7.82 (dd, 1H), 7.65 (m, 1H), 7.53 (t, 2H), 7.10 (d, 1H), 5.62 (s, 2H), 3.04 (t, 4H), 1.65 (m, 4H), 1.46 (m, 2H).
1H-NMR (CDCl3) δ: 8.56 (d, 1H), 8.45 (d, 2H), 7.64 (t, 1H), 7.52 (t, 2H), 7.38 (d, 1H), 5.60 (s, 2H), 3.21 (t, 4H), 1.54-1.66 (m, 4H), 1.51-1.53 (m, 2H).
1H-NMR (CDCl3) δ: 8.38 (s, 1H), 7.80 (dd, 1H), 7.64 (d, 1H), 7.49 (d, 1H), 7.13 (dd, 1H), 6.97 (d, 1H), 5.51 (s, 2H), 3.95 (s, 3H), 3.80 (s, 3H), 3.03 (t, 4H), 1.64 (m, 4H), 1.45 (m, 2H).
1H-NMR (CDCl3) δ: 8.38 (s, 1H), 7.82 (dd, 1H), 7.64 (d, 1H), 7.50 (m, 2H), 7.42 (t, 1H) 7.17 (dd, 1H), 5.60 (s, 2H), 3.86 (s, 3H), 3.03 (t, 4H), 1.64 (m, 4H), 1.45 (m, 211).
1H-NMR (CDCl3) δ: 8.38 (s, 1H), 7.92 (d, 1H), 7.82 (m, 2H), 7.60 (m, 2H), 7.45 (m, 1H), 5.57 (s, 21), 3.03 (t, 4H), 1.64 (m, 4H), 1.45 (m, 2H).
1H-NMR (CDCl3) δ: 8.41 (s, 1H), 7.95 (d, 2H), 7.80 (m, 1H), 7.64 (m, 1H), 7.52 (m, 2H), 7.44 (d, 1H), 5.60 (s, 2H), 3.01 (t, 4H), 2.72 (s, 3H), 1.64 (m, 4H), 1.45 (m, 2H).
1H-NMR (CDCl3) δ: 8.45 (dd, 1H), 7.96 (m, 3H), 7.62 (m, 1H), 7.52 (m, 2H), 7.34 (t, 1H), 5.63 (s, 2H), 3.02 (t, 4H), 1.66 (m, 4H), 1.45 (m, 2H).
1H-NMR (CDCl3) δ: 8.70 (d, 1H), 8.40 (s, 1H), 7.96 (d, 1H), 7.88 (dd, 1H), 7.82 (dd, 1H), 7.65 (d, 1H), 7.55 (m, 1H), 5.90 (s, 2H), 3.03 (t, 4H), 1.64 (m, 4H), 1.45 (m, 2H).
1H-NMR (CDCl3) δ: 8.28 (d, 1H), 7.98 (d, 2H), 7.79 (dd, 1H), 7.60 (dd, 2H), 7.50 (collapsed dd, 2H), 6.22 (q, 1H), 3.00 (t, 4H), 1.61-1.69 (m, 7H), 1.40-1.47 (m, 2H).
1H-NMR (CDCl3) δ: 8.39 (d, 1H), 7.93-8.03 (m, 2H), 7.64 (d, 1H), 7.31 (d, 2H), 5.59 (s, 2H), 3.03 (t, 4H), 2.43 (s, 1H), 1.62-1.69 (m, 4H), 1.38-1.48 (m, 2H).
1H-NMR (CDCl3) δ: 8.39 (s, 1H), 7.80-7.86 (m, 2H), 7.82 (d, 1H), 7.65 (d, 2H), 7.15-7.23 (m, 2H), 5.58 (s, 2H), 3.03 (t, 4H), 1.61-1.70 (m, 4H), 1.45-1.49 (m, 2H).
1H-NMR (CDCl3) δ: 8.31 (d, 1H), 7.81 (dd, 1H), 7.63 (d, 2H), 4.94 (s, 2H), 3.01 (t, 4H), 2.53 (q, 2H), 1.61-1.69(m, 4H), 1.44-1.48 (m, 2H), 1.14 (t, 3H).
1H-NMR (CDCl3) δ: 8.48 (s, 1H), 8.42 (d, 1H), 7.89-8.02 (m, 4H), 7.82 (dd, 1H), 7.57-7.66 (m, 3H), 5.75 (s, 2H), 3.04 (t, 4H), 1.62-1.70 (m, 4H), 1.39-1.49 (m, 2H).
1H-NMR (CDCl3) δ: 8.70 (d, 1H), 8.43 (s, 1H), 8.38 (s, 1H), 7.89 (d, 1H), 7.83 (dd, 1H), 7.65 (d, 1H), 7.43-7.54 (m, 2H), 5.59 (s, 2H), 3.05 (t, 4H), 1.61-1.71 (m, 4H), 1.39-1.50 (m, 2H).
1H-NMR (CDCl3) δ: 8.33 (s, 1H), 7.79 (dd, 1H), 7.62 (d, 1H), 5.15 (s, 2H), 3.02 (t, 4H), 1.61-1.69 (m, 4H), 1.44-1.48 (m, 2H), 1.26 (s, 9H).
1H-NMR (CDCl3) δ: 8.41 (d, 1H), 7.96 (d, 2H), 7.83 (dd, 1H), 7.62-7.70 (m, 2H), 7.52 (t, 2H), 5.63 (s, 2H), 3.76 (t, 4H), 3.06 (t, 4H).
1H-NMR (CDCl3) δ: 8.46 (d, 1H), 7.94 (dd, 2H), 7.88 (dd, 1H), 7.60-7.67 (m, 2H), 7.52 (t, 2H), 5.61 (s, 2H), 3.26 (q, 2H), 3.18 (t, 2H), 1.50-1.60 (m, 2H), 1.25-1.39 (m, 2H), 1.15 (t, 3H), 0.92 (t, 3H).
1H-NMR (CDCl3) δ: 8.43 (d, 1H), 7.94 (d, 2H), 7.85 (dd, 1H), 7.62-7.65 (m, 2H), 7.52 (t, 2H), 5.63 (s, 2H), 2.77 (s, 6H).
1H-NMR (CDCl3) δ: 8.39 (d, 1H), 7.94 (d, 2H), 7.81 (dd, 1H), 7.61 (t, 2H), 7.52 (t, 3H), 5.61 (s, 2H), 3.69 (d, 1H), 3.55 (d, 1H), 2.74-2.80 (m, 2H), 2.56 (dt, 1H), 2.34 (dt, 1H), 2.00-2.15 (m, 3H), 1.52-1.76 (m, 4H), 1.10-1.43 (m, 3H).
1H-NMR (CDCl3) δ: 8.39 (d, 1H), 7.95 (dd, 2H), 7.82 (dd, 1H), 7.61-7.67 (m, 2H), 7.50 (t, 2H), 5.62 (s, 2H), 3.77 (d, 2H), 2.31 (t, 2H), 1.69 (d, 2H), 1.22-1.32 (m, 3H), 0.88-0.93 (m, 3H).
1H-NMR (CDCl3) δ: 8.48 (s, 1H), 7.89-7.97 (m, 3H), 7.57-7.67 (m, 2H), 7.52 (t, 2H), 5.62 (s, 2H), 3.69-3.74 (m, 2H), 1.54-1.72 (m, 5H), 1.36 (d, 7H).
1H-NMR (CDCl3) δ: 8.48 (s, 1H), 8.33 (d, 1H), 7.94-7.99 (m, 3H), 7.61-7.68 (m, 2H), 7.51 (t, 2H), 5.62 (s, 2H), 3.01 (t, 4H), 1.61-1.68 (m, 4H), 1.38-1.46 (m, 2H).
Method B
To a mixture of the alkylaminosulfonylbenzoic acids in CH2Cl2 was added oxalyl chloride followed by DMF (one drop). The mixture was stirred at room temperature for 5 h. The solvent was then removed in vacuo and the residue was taken up into CH2Cl2 followed by the addition of amines or alcohol. The mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated onto silica and purified by flash column chromatography to afford the amides and esters.
1H-NMR (CDCl3) δ: 8.36 (s, 1H), 7.99 (d, 2H), 7.66 (m, 1H), 7.61 (s, 1H), 7.53 (t, 2H), 7.45 (br, t, 1H, —NH), 4.97 (d, 2H), 3.27 (t, 4H), 1.58 (m, 6H).
1H-NMR (CDCl3) δ: 8.19 (d, 1H), 8.01 (d, 2H), 7.66 (t, 1H), 7.53 (t, 2H), 7.42 (s, 1H), 7.29 (d, 1H), 4.97 (d, 2H), 3.18 (t, 4H), 1.61-1.68 (m, 4H), 1.27-1.42 (m, 2H).
Preparation of Piperazine HCl Salts
A mixture of the 2-oxo-2-phenethyl 2,4-dichloro-5-[4-tertbutyloxycarbonyl piperazinosulfonyl] benzoate in TFA/CH2Cl2 (1:1) was stirred at room temperature for 1 h after which NaHCO3 was added. The neutralized mixture was extracted with CH2Cl2 and concentrated in vacuo. The result was then dissolved in CH2Cl2 (2 mL) followed by HCl/ether (1M solution in ether) giving 51 mg of the HCl salt (74% yield).
1H-NMR (CDCl3/CD3OD) δ: 8.70 (s, 1H), 7.94 (d, 2H), 7.69 (s, 1H), 7.64 (t, 1H), 7.52 (t, 2H), 5.62 (s, 2H), 3.75 (br, m, 4H), 3.32 (br, m, 4H).
To a mixture of the 2,4-dichloro-5-[piperazinosulfonyl] benzoate hydrochloride in formic acid was added formaldehyde. The mixture was then stirred at room temperature for 1 h followed by heating at 120° C. for 4 h. The reaction mixture was then cooled and neutralized with saturated NaHCO3. The neutralized mixture was then extracted with CH2Cl2, wash with brine and dried over Na2SO4 (anhydrous). The organic layer was treated with HCl/ether (1M solution in ether) and concentrated in vacuo to afford the product as a white solid (25 mg, 11% overall yield from benzoic acid derivative).
1H-NMR (CDCl3/CD3OD) δ: 8.70 (s, 1H), 7.97 (d, 2H), 7.70 (s, 1H), 7.64 (t, 1H), 7.52 (t, 2H), 5.61 (s, 2H), 3.95 (br, m, 4H), 2.93 (s, 3H), 1.70 (br, m, 4H).
To a vigorously stirred solution of the 2,4-dichloro-5-[piperidinosulfonyl]benzoic acid (200 mg, 0.6 nmol) in THF (10 mL) was added isobutylchloroformate (121 mg, 0.89 mmol) followed by Et3N (1 mL). The reaction mixture was stirred at room temperature for 1 h after which the solvent was removed in vacuo. THF (5 mL) was then added followed by the addition of NaBH4 (114 mg, 3.0 mmol, dissolved in 2 mL of H2O). The reaction was stirred for a further 1 h and then quenched by the addition of methanol. The mixture was extracted with ethyl acetate, and the organic layer was washed with brine, and dried over MgSO4. The solvent was removed in vacuo and the residue was purified by flash chromatography giving 83 mg of a colourless oil (43% yield). The alcohol obtained was alkylated according to method A below to afford the corresponding ether (see below).
1H-NMR (CDCl3) δ: 8.25 (s, 1H), 7.93 (d, 2H), 7.64 (t, 1H), 7.55 (s, 1H), 7.52 (m, 2H) 4.90 (s, 2H), 4.75 (s, 2H), 3.25 (t, 4H), 1.60 (m, 6H).
To a solution of 2-chloro-5-(piperidine-1-sulphonyl)benzoyl chloride (80 mg, 0.248 mmol) in anhydrous toluene (2 mL) was added triethylamine (38 μL, 28 mg, 0.273 mmol), polystyrene-supported DMAP (17 mg of 1.46 mmol/g, 0.0248 mmol), and 2-hydroxy-2-methyl-1-phenylpropan-1-one (38 μL, 41 mg, 0.248 mmol). The mixture was heated to reflux. After 16 hours the cooled reaction mixture was filtered. The resin was washed with CH2Cl2. The filtrate was partitioned between saturated NaHCO3 and CH2Cl2. The organic phase was dried (MgSO4), filtered, and concentrated. Column chromatography (20% EtOAc/hexanes) provided 2-chloro-5-(piperidine-1-sulphonyl)benzoic acid 1,1-dimethyl-2-oxo-2-phenylethyl ester (20 mg, 18%) as a colourless oil.
1H NMR (CDCl3) δ: 8.02 (d, 2H), 7.85 (d, 1H), 7.74 (dd, 1H), 7.56 (d, 1H), 7.50 (collapsed dd, 1H), 7.39 (collapsed dd, 2H), 2.92-2.96 (t, 4H), 1.89 (s, 6H), 1.58-1.64 (m, 4H), 1.43-1.45 (m, 2H).
Method A
To a solution of alkylaminosulphonylphenylmethylbromide (50 mg, 0.142 mmol) in acentonitrile (3 mL) was added aminoacetic acid tert-butyl ester (36 mg, 0.213 mmol) and cesium carbonate (231 mg, 0.710 mmol). The reaction was allowed to stir for 1 hour before it was poured into water and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulphate and the solvent was removed in vacuo. Eluting through a solid phase extraction tube (Sg) with 5% ethyl acetate/hexanes afforded [2-chloro-5-(piperidine-1-sulphonyl)benzylamino]acetic acid tert-butyl ester (17.5 mg, 31%).
Method B
To a solution of 2-chloro-5-(piperidine-1-sulfonyl)benzaldehyde (80 mg, 0.28 mmol) and NaOAc (23 mg, 0.28 mmol) in MeOH (5 mL) was added glycine tert-butyl ester hydrochloride (113 mg, 0.67 mmol). The mixture was stirred at room temperature for 15 minutes after which NaBH3CN (11 mg, 0.17 mmol) was added, and the resulting mixture was stirred overnight. The mixture was diluted with ethyl acetate and then washed with saturated NaHCO3. The organic layer was washed with brine, dried over sodium sulphate and the solvent was removed in vacuo. Eluting through a solid phase extraction tube (5 g) with 5% ethyl acetate/hexanes afforded [2-chloro-5-(piperidine-1-sulphonyl)benzylamino]acetic acid tert-butyl ester (63 mg, 56%).
1H-NMR (CDCl3) δ: 7.95 (s, 1H) 7.63 (d, 1H), 7.53 (d, 1H) 4.17 (s, 2H), 3.52 (s, 2H), 2.99 (t, 4H), 1.53-1.61 (m, 4H), 1.45 (s, 9H), 1.39-1.42 (m, 2H).
To a solution of alkylaminosulphonylphenylmethyl bromide (50 mg, 0.142 mmol) in DMF (5 mL) was added tert-butyl sarcosine (75 mg, 0.4 mmol) and cesium carbonate (100 mg, 0.28 mmol). The reaction was allowed to stir at room temperature overnight before it was poured into water and extracted with CH2Cl2. The organic layer was washed with brine, dried over MgSO4 and the solvent was removed in vacuo. Eluting through a solid phase extraction tube (5 g) with 5% ethyl acetate/hexanes afforded the product (21 mg, 36%)
1H-NMR (CDCl3) δ: 7.93 (s, 1H) 7.52 (d, 1H), 7.47 (d, 1H) 3.88 (s, 2H), 3.24 (s, 2H), 2.99 (t, 4H), 2.42 (s, 3), 1.53-1.61 (m, 4H), 1.45 (s, 9H), 1.39-1.42 (m, 21).
Method A
To an ice-cold suspension of NaH (137 mg of a 60% dispersion in mineral oil, 3.42 mmol) in anhydrous THF (7 mL) was added a solution of (2-chloro-5-(piperidine-1-sulphonyl)phenyl)methanol (660 mg, 2.28 mmol) in anhydrous THF (7 mL). After 1 hour tert-butyl bromoacetate (505 μL, 667 mg, 3.42 mmol) was added. The reaction was allowed to slowly warm to room temperature. After 4 hours the reaction was quenched with saturated NH4Cl and poured into water. This was extracted with EtOAc. The organic phase was washed with brine, dried (Na2SO4), filtered, and concentrated. Column chromatography (20% EtOAc/hexanes) provided (2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)acetic acid tert-butyl ester (0.81 g, 88%) as a colourless oil.
1H NMR (CDCl3) δ: 7.94 (s, 1H), 7.61 (dd, 1H), 7.49 (d, 1H), 4.76 (s, 2H), 4.12 (s, 2H), 2.99 (t, 4H), 1.61-1.68 (m, 4H), 1.50 (s, 9H), 1.42-1.50 (m, 2H).
Method B
To a stirred solution of (2-chloro-5-(piperidine-1-sulphonyl)phenyl)methanol (100 mg, 0.345 mmol), cesium carbonate (224.8 mg, 0.690 mmol) in anhydrous acetonitrile (6 mL) was added at 0° C. a solution of 2-bromopropionic acid tert-butyl ester (144 mg, 0.690 mmol) in anhydrous acetonitrile (1 mL). The reaction was allowed to slowly warm to room temperature. After 15 min, the reaction mixture was refluxed at 100° C. for 3 hours. The reaction was allowed to stir for 30 min before it was poured into water and extracted with ethyl acetate. The organic layer was washed with brine, dried over sodium sulphate and the solvent was removed in vacuo. After purification on silica gel with 30% ethyl acetate/hexanes afforded the title compound as viscous oil (85.5 mg, 59.9%).
1H NMR (CDCl3) δ: 7.98 (s, 1H), 7.61 (dd, 1H), 7.45 (d, 1H), 4.67 (dd, 2H), 4.05 (q, 1H), 3.00 (t, 4H), 1.78-1.60 (m, 4H), 1.48 (s, 9H), 1.47 (d, 3H), 1.49-1.40 (m, 2H).
In a similar fashion the following compound was made.
2-[2-chloro-5-(piperidine-1-sulfonyl)benzyloxy]butyric acid tert-butyl ester was prepared (10.7 mg, 17.2%) from tert-butyl (R)-2-hydroxybutyrate (50 mg, 0.141 mmol) and piperidinosulphonylphenylmethyl bromide (45.5 mg, 0.283 mmol).
1H NMR (CDCl3) δ: 7.95 (s, 1H), 7.62 (dd, 1H), 7.45 (d, 1H), 4.65 (dd, 2H), 3.83 (t, 1H), 3.02 (t, 4H), 2.01-1.81 (m, 2H), 1.78-1.61 (m, 4H), 1.52 (s, 9H), 1.1.50-1.40 (m, 2H), 1.05 (t, 3H).
(2-Chloro-5-(piperidine-1-sulphonyl)benzyloxy)acetic acid tert-butyl ester (100 mg) was dissolved in 96% formic acid (1 mL). After 3 hours the reaction mixture was concentrated to provide (2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)acetic acid (86 mg, 100%) as a colourless solid. 1H NMR (CDCl3) δ: 7.91 (s, 1H), 7.65 (dd, 1H), 7.52 (d, 1H), 4.80 (s, 2H), 4.31 (s, 2H), 3.00 (t, 4H), 1.63-1.65 (m, 4H), 1.42-1.44 (m, 2H).
Method A
To a solution of (2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)acetic acid (50 mg, 0.144 mmol) in anhydrous CH2Cl2 was added EDCI (30 mg, 0.158 mmol) and pyrrolidine (13 μL, 11 mg, 0.158 mmol). After 16 hours the reaction mixture was directly loaded onto a 5 g silica SPE tube and eluted with CH2Cl2 and then EtOAc. The EtOAc fraction was concentrated to provide 2-(2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)-1-pyrrolidin-1-ylethanone (36 mg, colourless oil)
1H NMR (CDCl3) δ: 7.91 (d, 1H), 7.60 (dd, 1H), 7.48 (d, 1H), 4.77 (s, 2H), 4.23 (s, 2H), 3.50 (t, 2H), 3.40 (t, 2H), 2.97 (t, 4H), 1.80-2.03 (m, 4H), 1.59-1.66 (m, 4H), 1.37-1.48 (m, 2H).
In a similar fashion the following compounds (Examples 42-46) were prepared.
1H NMR (CDCl3) δ: 7.84 (s, 1H), 7.58 (dd, 1H), 7.33-7.46 (m, 4H), 7.21 (d, 2H), 4.69 (s, 2H), 4.01 (s, 2H), 3.30 (s, 3H), 2.95 (t, 4H), 1.59-1.68 (m, 4H), 1.40-1.49 (m, 2H).
1H NMR (CDCl3) δ: 7.82 (d, 1H), 7.65 (dd, 1H), 7.54 (d, 1H), 6.37 (br s, 1H), 4.69 (s, 2H), 3.97 (s, 2H), 2.99 (t, 4H), 1.58-1.68 (m, 4H), 1.37-1.49 (m, 2H), 1.37 (s, 9H).
1H NMR (CDCl3) δ: 7.90 (s, 1H), 7.62 (d, 1H), 7.50 (d, 1H), 4.77 (d, 2H), 4.69 (br s, 1H), 4.22 (d, 2H), 4.03-4.15 (m, 1H), 3.68-3.76 (m, 1H), 3.49-3.64 (m, 2H), 3.35 (ddd, 1H), 2.98 (t, 4H), 2.11-2.23 (m, 1H), 1.76-2.04 (m, 1H), 1.60-1.67 (m, 5H), 1.47-1.51 (m, 10H).
1H NMR (CDCl3) δ: 7.88 (d, 1H), 7.61 (dd, 1H), 7.50 (d, 1H), 4.73 (s, 2H), 4.46-4.52 m, 2H), 4.30 (dd, 2H), 4.03-4.15 (m, 1H), 3.84 (d, 1H), 3.66 (br s, 1H), 3.10 (t, 1H), 2.98 (t, 4H), 2.77 (t, 1H), 1.98 (d, 2H), 1.60-1.66 (m, 4H), 1.43 (s, 9H), 1.25-1.43 (m, H).
1H NMR (CDCl3) δ: 8.88 (br s, 1H), 8.29 (dd, 1H), 8.24 (s, 1H), 7.90 (d, 1H), 7.69-7.72 (m, 2H), 7.58 (d, 1H), 7.10 (dd, 1H), 4.82 (s, 2H), 4.26 (s, 2H), 3.03-3.07 (m, 4H), 1.64-1.72 (m, 4H), 1.25-1.30 (m, 2H).
Method B
To a suspension of PS-carbodiimide (578 mg, 0.728 mmol) in anhydrous dichloromethane (8 mL) was added piperidine (24 mg, 0.287 mmol) and (2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)acetic acid (25 mg, 0.0718 mmol) and stirred for 12 hours. The solvent was removed in vacuo and the compound was purified by eluting through a solid phase extraction tube (5 g) with 5% methanol/dichloromethane to afford 2-[2-chloro-5-piperidine-1-sulphonyl)benzyloxy]-1-piperidin-1-ylethanone (17 mg, 57%)
1H-NMR (CDCl3) δ: 7.90 (d, 1H), 7.61 (dd, 1H), 7.49 (d, 1H), 4.74 (s, 2H), 4.31 (s, 2H), 3.56 (t, 2H), 3.41 (t, 2H), 2.98 (t, 4H), 1.56-1.67 (m, 8H), 1.42-1.49 (m, 2H).
To a suspension of NaH (11.43 mg of 60% in mineral oil, 0.286 mmol) in anhydrous THF (2 mL) at 0° C. was added 2-[2-Chloro-5-(piperidine-1-sulfonyl)-benzyloxy]-N-pyridin-2-yl-acetamide (110 mg, 0.26 mmol) in THF (4 mL). After 30 minutes iodomethane (41 mg, 0.286 mmol) was added. The mixture was stirred at room temperature for 16 hours then poured into water and extracted with EtOAc. The organic phase was washed with brine, dried (Na2SO4), filtered, and concentrated. Column chromatography (50% EtOAc/hexanes) provided the product as a yellow oil (44 mg, 39% yield)
1H-NMR (CDCl3) δ: 8.42 (dd, 1H), 7.75-7.78 (m, 2H), 7.58 (d, 1H), 7.46 (d, 1H), 7.28 (s, 1H), 7.20 (dd, 1H), 4.71 (s, 2H), 4.42 (s, 2H), 3.42 (s, 3H), 2.93-2.99 (m, 4H), 1.61-1.68 (m, 4H), 1.39-1.46 (m, 2H).
To an ice-cold suspension of NaH (185 mg of a 60% dispersion in mineral oil, 4.62 mmol) in anhydrous THF (10 mL) was added a solution of (2,4-dichloro-5-(piperidine-1-sulphonyl)phenyl)methanol (1.0 g, 3.08 mmol) in anhydrous THF (10 mL). After 1 hour a solution of 2-chloro-N-methoxy-N-methylacetamide (635 mg, 4.62 mmol) in anhydrous THF (10 mL) was added. The reaction was allowed to slowly warn to room temperature. After 2 hours the reaction was quenched with saturated NH4Cl and poured into water. This was extracted with EtOAc. The organic phase was washed with brine, dried (Na2SO4), filtered, and concentrated. Column chromatography (45% EtOAc/hexanes) provided 2-(2,4-dichloro-5-(piperidine-1-sulphonyl)benzyloxy)-N-methoxy-N-methylacetamide (1.31 g, 72%) as a colourless solid
1H NMR (CDCl3) δ: 8.15 (s, 1H), 7.45 (s, 1H), 4.68 (s, 2H), 4.36 (s, 2H), 3.64 (s, 3H), 3.14-3.20 (m, 4H), 3.14 (s, 3H), 1.46-1.55 (m, 6H).
In a similar fashion the following compounds (Examples 50-56) were prepared.
1H NMR (CDCl3) δ: 7.94 (d, 1H), 7.58 (dd, 1H), 7.47 (d, 1H), 4.78 (s, 2H), 4.42 (s, 2H), 3.68 (s, 3H), 3.19 (s, 3H), 2.96 (t, 4H), 1.58-1.65 (m, 4H), 1.36-1.43 (m, 2H).
1H NMR (CDCl3) δ: 7.72 (s, 1H), 7.58 (dd, 1H), 6.89 (d, 1H), 4.63 (s, 2H), 4.29 (s, 2H), 3.81 (s, 3H), 3.60 (s, 3H), 3.11 (s, 3H), 2.86 (t, 4H), 1.50-1.58 (m, 4H), 1.27-1.35 (m, 2H).
1H NMR (CDCl3) δ: 7.73 (s, 1H), 7.55 (d, 1H), 7.29 (d, 1H), 4.68 (s, 2H), 4.33 (s, 2H), 3.65 (s, 3H), 3.18 (s, 3H), 2.93 (t, 4H), 2.41 (s, 3H), 1.57-1.64 (m, 4H), 1.33-1.41 (m, 2H).
1H NMR (CDCl3) δ: 7.96 (s, 1H), 7.70 (d, 2H), 7.54 (dd, 1H), 4.79 (s, 2H), 4.46 (s, 2H), 3.72 (s, 3H), 3.24 (s, 3H), 3.01 (t, 4H), 2.41 (s, 3H), 1.63-1.70 (m, 4H), 1.41-1.48 (m, 2H).
1H NMR (CDCl3) δ: 8.05 (s, 1H), 7.74 (d, 1H), 7.36 (dd, 1H), 4.80 (s, 2H), 4.42 (s, 2H), 3.71 (s, 3H), 3.23 (s, 3H), 3.01 (t, 4H), 1.63-1.70 (m, 4H), 1.41-1.49 (m, 2H).
1H NMR (CDCl3) δ: 1.47 (m, 2H), 1.56 (d, 3H), 1.66 (m, 4H), 3.01 (t, 4H), 3.20 (s, 3H), 3.64 (s, 3H), 4.06 (d, 1H), 4.29 (d, 1H), 5.08 (q, 1H), 7.50 (d, 1H), 7.64 (d, 1H), 7.97 (s, 1H).
1H NMR (CDCl3) δ: 7.98(d, 1H), 7.62 (dd, 1H), 7.52 (d, 1H), 4.82 (s, 2H), 4.46 (s, 2H), 3.72 (s, 3H), 3.35-3.38 (m, 4H), 3.23 (s, 3H), 2.71-2.74 (m, 4H).
Method A: Using Commercially Available Grignard Reagent Solutions
To an ice-cold solution of 2-(2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)-N-methoxy-N-methylacetamide (80 mg, 0.205 mmol) in anhydrous THF (3 mL) was added phenylmagnesium bromide (205 μL of a 1.0M solution in THF, 0.205 mmol). After 1 hour more phenylmagnesium bromide (50 μL of a 1.0M solution in THF, 0.050 mmol) was added. After an additional hour the reaction was quenched with saturated NH4Cl and poured into water. This was extracted with EtOAc. The organic phase was washed with brine, dried (MgSO4), filtered, and concentrated. Column chromatography (20% EtOAc/hexanes) provided 2-(2-chloro-5-(piperidine-1-sulphonyl)benzyloxy)-1-phenylethanone (28 mg, 34%) as a colourless oil. 1H NMR (CDCl3) δ: 7.93-7.96 (m, 3H), 7.58-7.64 (m, 2H), 7.46-7.51 (m, 3H), 4.92 (s, 2H), 4.82 (s, 2H), 2.98 (t, 4H), 1.59-1.67 (m, 4H), 1.41-1.43 (m, 2H).
In a similar fashion, the following compounds (Example 57-70) were made.
1H-NMR (CDCl3) δ: 7.96 (d, 1H), 7.60 (dd, 1H), 7.47 (dd, 3H), 7.37 (t, 1H), 7.13 (dd, 1H), 4.90 (s, 2H), 4.81 (s, 1H), 3.80 (s, 3H), 2.97 (t, 4H), 1.59-1.66 (m, 4H), 1.40-1.44 (m, 2H).
1H-NMR (CDCl3) δ: 7.91 (d, 1H), 7.62 (dd, 1H), 7.49 (d, 1H), 4.70 (s, 2H), 4.28 (s, 2H), 2.99 (t, 4H), 2.47-2.56 (m, 1H), 1.80 (t, 4H), 1.60-1.68 (m, 4H), 1.18-1.44 (m, 7H).
1H-NMR (CDCl3) δ: 7.99 (d, 1H), 7.61 (dd, 1H), 7.46 (m, 2H), 7.08 (dd, 1H), 6.92 (d, 1H), 4.86 (s, 2H), 4.81 (s, 2H), 3.89 (s, 3H), 3.81 (s, 3H), 2.99 (t, 4H), 1.63 (m, 4H), 1.42 (m, 2H).
1H-NMR (CDCl3) δ: 8.22 (s, 1H), 7.46-7.52 (m, 3H), 7.38 (t, 1H), 7.13 (dd, 1H), 4.89 (s, 2H), 4.75 (d, 2H), 3.85 (s, 3H), 3.25 (t, 4H), 1.53-1.61 (m, 6H).
1H-NMR (CDCl3) δ: 7.93 (m, 2H), 7.83 (dd, 1H), 7.62 (dd, 1H), 7.58 (dd, 1H), 7.55-7.40 (m, 2H), 4.88 (s, 2H), 4.80 (s, 1H), 2.98 (t, 4H), 1.63 (m, 4H), 1.42 (m, 2H).
1H-NMR (CDCl3) δ: 8.20 (s, 1H), 7.91 (s, 1H), 7.81 (s, 1H), 7.54 (dd, 2H), 7.43 (t, 1H), 4.91 (s, 2H), 4.74 (s, 1H), 3.25 (t, 4H), 1.53-1.63 (m, 6H).
1H-NMR (CDCl3) δ: 8.47 (s, 1H), 8.26 (s, 1H), 7.86-8.01 (m, 4H), 7.51-7.64 (m, 2H), 7.43 (t, 1H), 5.02 (s, 2H), 4.80 (s, 1H), 3.24 (t, 4H), 1.51-1.59 (m, 6H).
1H-NMR (CDCl3) δ: 8.48 (s, 1H), 8.02-7.87 (m, 5H), 7.64-7.48 (m, 4H), 5.05 (s, 2H), 4.86 (s, 2H), 2.96 (t, 4H), 1.60 (m, 4H), 1.40 (m, 2H).
1H-NMR (CDCl3) δ: 8.00 (s, 1H), 7.93 (d, 2H), 7.57-7.66 (m, 2H), 7.45-7.52 (m, 3H), 4.92 (s, 2H), 4.82 (s, 2H), 2.71(s, 6H).
1H-NMR (CDCl3) δ: 8.04 (s, 1H), 7.96 (dd, 2H), 7.70 (dd, 1H), 7.59-7.62 (m, 1H), 7.48 (t, 3H) 4.92 (s, 2H), 4.82 (s, 2H), 3.25 (q, 4H), 1.15(t, 6H).
1H-NMR (CDCl3) δ: 8.02 (s, 1H), 7.96 (dd, 2H), 7.59-7.66 (m, 2H), 7.47-7.52 (m, 3H) 4.94 (s, 2H), 4.83 (s, 2H), 3.12 (q, 2H), 2.76 (s, 3H), 1.15(t, 3H).
1H-NMR (CDCl3) δ: 8.02 (s, 1H), 7.95 (dd, 2H), 7.59-7.66 (m, 2H), 7.47-7.52 (m, 3H) 4.94 (s, 2H), 4.83 (s, 2H), 2.99 (t, 2H), 2.75 (s, 3H), 1.57 (q, 2H), 0.95(t, 3H).
1H-NMR (CDCl3) δ: 7.94 (t, 3H), 7.69 (d, 1H), 7.45-7.60 (m, 4H), 4.94 (s, 2H), 4.78 (s, 2H), 2.97 (t, 4H), 1.55-1.66 (m, 4H), 1.36-1.44(m, 2H).
1H-NMR (CDCl3) δ: 7.95-8.01 (m, 3H), 7.62-7.66 (m, 2H), 7.49-7.61 (m, 3H), 4.95 (s, 2H), 4.85 (s, 2H), 3.35-3.38 (m, 4H), 2.71-2.74 (m, 4H).
To a solution of 1H-pyrrolo(2,3-b)-pyridine (71 mg, 0.6 mmol) in THF (2 ml) was added a Et2O solution of t-BuMgBr (2M, 0.33 ml, 0.66 mmol) at −50° C. and stirred at room temperature for 10 minutes. The mixture was cooled to −50° C. and was added a solution of 2-(2-chloro-5-(piperidine-1-sulfonyl)-benzyloxyl)-acetyl chloride (200 mg, 0.6 mmol), prepared from corresponding acetic acid, oxylchloride, catalytic amount of DMF and CH2Cl2, in THF (2 ml) at this temperature and stirred at room temperature for 1 h. To this resulting mixture was added H2O (2 ml) and extracted with CH2Cl2 (5 ml×3). The combined extract was dried and the solvent was removed under reduced pressure to leave residue which was purified on silica gel colum to provide the product (12 mg, 10%)
1H NMR (CDCl3): δ 1.46 (m, 2H), 1.61 (m, 4H), 3.00 (t, 4H), 4.73 (s, 2H), 4.86 (s, 2H), 7.32-7.67 (m, 3H), 8.02 (s, 1H), 8.40 (s, 1H), 8.50 (m, 1H), 8.73 (d, 1H).
Method B: Using Lithium/Halogen Exchange
To a solution of 2-bromopyridine (80 mg, 0.5 mmol) in Et2O (2 ml) was added n-BuLi (1.6 M, 0.35 ml, 0.55 mmol) in hexane at −78° C. The mixture was stirred at this temperature for 1 h. To this was added a solution of 4-{4-chloro-3-[(methoxymethylcarbamoyl)methoxymethyl]benzenesulfonyl}piperazine-1-carboxylic acid tert-butyl ester (200 mg, 0.4 mmol) in THF (1 ml) at −78° C. and was kept stirring at this temperature for 2 hours. To this resulting mixture was added H2O (1 ml) at rt and extracted with AcOEt (3×3 ml). The combined extract was dried and concentrated under reduced pressure to afford residue. This residue was treated with CH2Cl2:TFA (1:1, 2 ml) at 0° C. for 30 minutes. The resulting mixture was neutralized with saturated Na2CO3 aq and extracted with AcOEt (5×5 ml). The combined extract was dried and the solvent was removed under reduced pressure to afford residue, which was purified on silica gel column to give the deprotected intermediate 2-[2-chloro-5-(piperazine-1-sulfonyl)benzyloxy]-N-methoxy-N-methylacetamide (92 mg). The intermediate was diluted with formic acid (0.6 ml) and was added H2CO (37% aq, 1.2 ml) and a solution of NaCNBH3 in THF (1 M, 0.16 ml, 0.16 mmol) at 0° C. The mixture was stirred at 0° C. for 20 minutes. The resulting mixture was neutralized with saturated Na2CO3 aq and extracted with AcOEt (5×5 ml). The combined extract was dried and concentrated under reduced pressure to give residue, which was purified on silica gel column to afford the product (11 mg, 6.5% allover yield)
1H NMR (CDCl3) δ: 2.275 (s, 3H), 2.464 (t, J=5.0 Hz, 4H), 3.062 (t, J=5.0 Hz, 4H), 4.875 (s, 2H), 5.250 (s, 2H), 7.535 (m, 2H), 7.640 (d, J=8.6 Hz, 1H), 7.888 (m, 1H), 8.074 (m, 2H), 8.644 (s, 1H).
In a similar fashion, the following compounds (Examples 73-101) were synthesized.
1H-NMR (CDCl3) δ: 9.14 (s, 1H), 8.80 (s, 1H), 8.23 (dd, 1H), 7.92 (s, 1H), 7.60 (dd, 1H), 7.42-7.50 (m, 2H), 4.89 (s, 2H), 4.80 (s, 1H), 2.97 (t, 4H), 1.59-1.66 (m, 6H), 1.41-1.45 (m, 4H).
1H-NMR (CDCl3) δ: 8.85 (d, 2H), 7.96 (d, 1H), 7.74 (dd, 2H), 7.66 (dd, 1H), 7.53 (d, 1H), 4.92 (s, 2H), 4.87 (s, 2H), 3.03 (t, 4H), 1.63-1.70 (m, 6H), 1.41-1.49 (m, 4H).
1H-NMR (CDCl3) δ: 8.32 (d, 1H), 8.10 (d, 1H), 8.03 (s, 1H), 7.89 (t, 1H), 7.64 (dd, 1H), 7.51-7.54 (m, 2H), 5.26 (s, 2H), 4.87 (s, 2H), 3.02 (t, 4H), 1.62-1.69 (m, 6H), 1.40-1.47 (m, 4H).
1H-NMR (CDCl3) δ: 9.19 (s, 1H), 8.85 (s, 1H), 8.30 (dd, 1H), 7.99 (s, 1H), 7.68 (dd, 1H), 7.48-7.56 (m, 2H), 4.92 (s, 2H), 4.87 (s, 2H), 2.74 (s, 611).
1H-NMR (CDCl3) δ: 8.96 (t, 1H), 8.20 (t, 2H), 7.97 (t, 2H), 7.60-7.67 (m, 2H), 7.48-7.51 (m, 2H), 5.37 (s, 2H), 4.92 (s, 2H), 2.98 (t, 4H), 1.58-1.66 (m, 4H), 1.36-1.44 (m, 6H).
1H-NMR (CDCl3) δ: 8.65 (d, 1H), 8.09 (d, 2H), 7.89 (t, 1H), 7.67 (dd, 1H), 7.51-7.55 (m, 2H), 5.27 (s, 2H), 4.88 (s, 2H), 2.75 (s, 6H).
1H-NMR (CDCl3) δ: 8.64 (d, 1H), 8.07 (d, 2H), 7.89 (t, 1H), 7.66 (dd, 1H), 7.49-7.55 (m, 2H), 5.24 (s, 2H), 4.86 (s, 2H), 3.13 (q, 2H), 2.77 (s, 6H), 1.18 (t, 3H).
1H-NMR (CDCl3) δ: 8.64 (d, 1H), 8.08 (d, 2H), 7.89 (t, 1H), 7.70 (dd, 1H), 7.47-7.55 (m, 2H), 5.25 (s, 2H), 4.86 (s, 2H), 3.27 (q, 4H), 1.16 (t, 6H).
1H-NMR (CDCl3) δ: 8.64 (d, 1H), 8.07 (d, 2H), 7.85 (t, 1H), 7.65 (dd, 1H), 7.50-7.55 (m, 2H), 5.25 (s, 2H), 4.86 (s, 2H), 2.99 (t, 2H), 2.75 (s, 3H), 1.63-1.51 (m, 2H), 0.93 (t, 6H).
1H-NMR (CDCl3) δ: 9.16 (d, 1H), 8.82 (dd, 1H), 8.26 (dd, 1H), 7.98 (d, 1H), 7.67 (dd, 1H), 7.46-7.50 (m, 2H), 4.91 (s, 2H), 4.82 (s, 2H), 3.12 (q, 2H), 2.75 (s, 3H), 1.15 (t, H).
1H-NMR (CDCl3) δ: 9.17 (d, 1H), 8.83 (dd, 1H), 8.27 (dd, 1H), 8.01 (d, 1H), 7.70 (dd, 1H), 7.45-7.47 (m, 2H), 4.90 (s, 2H), 4.82 (s, 2H), 3.24 (q, 4H), 1.15 (t, 6H).
1H-NMR (CDCl3) δ: 8.49 (s, 1H), 8.02 (s, 1H), 7.90 (s, 1H), 7.64 (dd, 1H), 7.51 (d, 1H), 7.33 (d, 1H), 5.23 (s, 2H), 4.86 (s, 2H), 3.01 (t, 4H), 2.45 (s, 3H), 1.60-1.68 (m, 4H), 1.39-1.46 (m, 2H).
1H-NMR (CDCl3) δ: 8.03 (s, 1H), 7.87 (d, 1H), 7.74 (t, 1H), 7.64 (dd, 1H), 7.51 (d, 1H), 7.37 (d, 1H), 5.26 (s, 2H), 4.87 (s, 2H), 3.01 (t, 4H), 2.59 (s, 3H), 1.61-1.68 (m, H), 1.39-1.46 (m, 2H).
1H-NMR (CDCl3) δ: 8.45 (s, 1H), 8.02 (s, 1H) 7.98 (d, 1H), 7.65 (t, 2H), 7.52 (d, 1H), 5.23 (s, 2H), 4.87 (s, 2H), 3.01 (t, 4H), 2.43 (s, 3H), 1.61-1.68 (m, 4H), 1.39-1.46 (m, 2H).
1H-NMR (CDCl3) δ: 8.48 (d, 1H), 8.05 (s, 1H) 7.90 (s, 1H), 7.66 (d, 2H), 7.51 (d, 1H), 7.32 (dd, 1H), 5.23 (s, 2H), 4.85 (s, 21]), 3.13 (q, 2H), 2.76 (s, 3H), 2.46 (s, 3H), 1.15 (t, 31].
1H-NMR (CDCl3) δ: 8.07 (s, 1H), 7.88 (d, 1H) 7.66-7.86 (m, 2H), 7.51 (d, 1H), 7.37 (d, 1H), 5.26 (s, 2H), 4.86 (s, 2H), 3.14 (q, 2H), 2.80 (s, 3H), 2.59 (s, 3H), 1.15 (t, 3H).
1H-NMR (CDCl3) δ: 8.44 (s, 1H), 8.05 (s, 1H) 7.97 (d, 1H), 7.66 (dd, 2H), 7.49 (d, 1H), 5.22 (s, 2H), 4.85 (s, 2H), 3.12 (q, 2H), 2.76 (s, 3H), 2.43 (s, 3H), 1.15 (t, 3H).
1H-NMR (CDCl3) δ: 8.47 (d, 1H), 8.04 (s, 1H) 7.64 (d, 2H), 7.52 (d, 1H), 7.39 (dd, 1H), 5.20 (s, 2H), 4.87 (s, 2H), 3.02 (t, 4H), 2.66 (s, 3H), 1;59-1.69 (m, 4H), 1.39-1.47 (m, 2H).
1H-NMR (CDCl3) δ: 8.47 (d, 1H), 8.07 (s, 1H) 7.65-7.69 (m, 2H), 7.51 (d, 2H), 7.39 (dd, 1H), 5.20 (s, 2H), 4.86 (s, 2H), 3.14 (q, 2H), 2.77 (s, 3H), 2.66 (s, 3H), 1.16 (t, 3H).
1H-NMR (CDCl3) δ: 8.16 (s, 1H), 8.86 (s, 1H) 7.64 (d, 1H), 7.53 (d, 1H), 7.34 (s, 1H), 4.80 (s, 2H), 4.76 (s, 2H), 2.98 (t, 4H), 2.86 (s, 3H), 1.63-1.70 (m, 4H), 1.41-1.48 (m, 2H).
1H-NMR (CDCl3) δ: 8.62 (d, 1H), 8.07 (d, 1H) 7.87 (t, 2H), 7.70 (d, 1H), 7.52 (dd, 1H), 6.96 (d, 1H), 5.19 (s, 2H), 4.80 (s, 2H), 3.90 (s, 3H), 2.97 (s, 3H), 1.59-1.73 (m, 4H), 1.27-1.43 (m, 2H).
1H-NMR (CDCl3) δ: 8.64 (d, 1H), 8.08 (d, 1H) 7.89 (t, 1H), 7.81 (s, 1H), 7.61 (d, 1H), 7.54 (dd, 1H), 7.31 (d, 1H), 5.18 (s, 2H), 4.79 (s, 2H), 2.49 (s, 3H), 1.57-1.68 (m, 4H), 1.25-1.30 (m, 2H).
1H-NMR (CDCl3) δ: 8.65 (d, 1H), 8.09 (d, 2H) 7.89 (t, 1H), 7.74 (d, 1H), 7.55 (dd, 1H), 7.41 (d, 1H), 5.24 (s, 2H), 4.86 (s, 2H), 3.05(t, 3H), 1.63-1.71 (m, 4H), 1.41-1.49 (m, 2H).
1H-NMR (CDCl3) 6:1.40 (m, 2H), 1.52-1.64 (m, 7H), 2.98 (t, 4H), 4.93-5.15 (m, 3H), 7.49-7.64 (m, 3H), 7.87 (d, 1H), 8.02 (s, 1H), 8.06 (d, 1H), 8.56 (m, 1H).
1H-NM (CDCl3) 8:1.44 (m, 2H), 1.51-1.63(m, 7H), 3.00 (t, 4H), 4.60 (d, 1H), 4.71 (d, 1H), 5.09 (q, 1H), 7.46-7.66 (m, 3H), 7.96 (s, 1H), 8.22 (m, 1H), 8.82 (d, 1H), 9.12 (s, 1H).
1H-NMR (CDCl3) δ: 1.46 (m, 2H), 1.66 (m, 4H), 3.03(t, 4H), 4.88 (s, 2H), 5.21 (s, 2H), 7.52-7.67 (m, 2H), 8.03 (s, 1H), 8.64 (d, 1H), 8.83 (d, 1H), 9.27 (s, 1H).
1H-NMR (CDCl3) δ: 1.44 (m, 2H), 1.66 (m, 4H), 3.02 (t, 4H), 4.06 (s, 3H), 4.85 (s, 2H), 5.09 (s, 2H), 7.10 (d, 1H), 7.15 (d, 1H), 7.50-7.66 (m, 2H). 8.02 (s, 1H).
2-(2-chloro-5-(piperidine-1-sulfonyl)-benzyloxy)-1-(1-pyridin-2-yl-1H-imidazol-2-yl)ethanone (75 mg, 59% yield) was prepared from 2-imidazol-1-yl-pyridine instead of its bromide in a similar procedure
1H-NMR (CDCl3) δ: 1.43 (m, 2H), 1.65 (m, 4H), 2.98 (t, 4H), 4.80 (s, 2H), 5.14 (s, 2H), 7.28 (d, 1H), 7.44-7.61 (m, 5H), 7.90 (m, 1H), 7.97 (s, 1H), 8.58 (d, 1H)
1H-NMR (CDCl3) δ: 1.44 (m, 2H), 1.66 (m, 4H), 3.00 (t, 4H), 3.86 (s, 3H), 4.87 (s, 2H), 5.02 (s, 2H), 6.63 (d, 1H), 7.15 (d, 1H), 7.38 (d, 1H), 7.51 (d, 1H), 7.63 (d, 1H), 7.89 (d, 1H) 8.02 (s, 1H), 8.31 (s, 1H).
A mixture of 2-[2-chloro-5-(piperidine-1-sulfonyl)benzyloxy]propionic acid tert-butyl ester (171 mg, 0.4 mmol) and HCO2H (1.5 ml) was stirred at room temperature overnight. HCO2H was removed under reduced pressure to give the acid, which was treated with oxalyl chloride (2M, 0.4 ml, 0.8 mmol) in CH2Cl2 (3 ml) in the presence of catalytic amount of DMF at room temperature for 2 hours to afford corresponding acetyl chloride. The acetyl chloride was diluted with CH2Cl2 (5 ml) and cooled to 0° C. To this were added ClH2N(OMe)Me (48 mg, 0.5 mmol) and Et3N (155 μl, 1.3 mmol) at this temperature and kept stirring for 1 h. To this resulting mixture was added H2O (1 ml) and extracted with CH2Cl2 (5 ml×3). The combined extract was dried and the solvent was removed under reduced pressure to give the amide which was treated with 2-lithio-pyridine in a similar procedure mentioned above to afford the final product (106 mg, 61%)
1H-NMR (CDCl3) δ: 1.46 (m, 2H), 1.53-1.64 (m, 7H), 3.01 (t, 4H), 4.51 (d, 1H), 4.85 (d, 1H), 5.59 (q, 1H), 7.47-7.64 (m, 3H), 7.89 (d, 1H), 8.01 (s, 1H), 8.09(d, 1H), 8.69 (m, 1H).
To a solution of magnesium turnings (8.0 equiv) in THF was added 1,2-dibromoethane (2 drops). 1-Bromo-3-methoxymethylbenzene (1.0 equiv) dissolved in THF was added dropwise and the reaction stirred at room temperature for 2 hours. The Grignard solution was added dropwise to the allcylaminosulphonylbenzyloxy-N-methoxy-N-methylacetamide dissolved in THF at 0° C. After stirring for an additional 30 minutes the reaction was quenched with water and diluted with ethyl acetate and separated. The organic was washed with brine and dried over anhydrous sodium sulphate. The organic solvent was removed in vacuo and the final product was purified by eluting through a solid phase extraction tube (10 g) with 30% ethyl acetate/hexanes.
1H-NMR (CDCl3) δ: 8.22 (s, 1H), 7.87 (t, 2H), 7.57 (d, 1H), 7.44-7.52 (m, 2H), 4.91 (s, 2H), 4.75 (s, 2H), 4.50 (s, 2H), 3.41 (s, 3H), 3.25 (t, 4H), 1.52-1.61 (m, 6H).
1H-NMR (CDCl3) δ: 8.24 (s, 1H), 7.94 (dd, 2H), 7.54 (s, 1H), 7.46 (d, 2H), 4.91 (s, 2H), 4.77 (s, 2H), 4.54 (s, 2H), 3.44 (s, 3H), 3.27 (t, 4H), 1.52-1.63 (m, 6H).
1H-NMR (CDCl3) δ: 8.25 (s, 1H), 7.83-7.88 (m, 2H), 7.57 (t, 2H), 7.45 (t, 1H), 7.43 (t, H), 4.94 (s, 2H), 4.77 (s, 2H), 3.49 (s, 2H), 3.27 (t, 4H), 2.26 (61, 1.55-1.63 (m, 611.
1H-NMR (CDCl3) δ: 7.96 (s, 1H), 7.84 (m, 2H), 7.63-7.40 (m, 4H), 4.93 (s, 2H), 4.81 (s, 2H), 3.46 (s, 2H), 2.98 (t, 4H), 2.23 (6H), 1.63 (m, 4H), 1.42 (m, 2H).
To a mixture of 2-[2-bromo-5(piperidine-1-sulphonyl)-benzyloxy]-1-phenyl-ethanone (0.215 g, 0.475 mmol) in N-methylpyrrolidinone (3.0 mL), was added zinc cyanide (0.039 g, 0.333 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.055 g, 0.0475 mmol). The reaction flask was sealed and stirred at 80° C. overnight. The reaction mixture was cooled to R.T. and extracted with AcOEt (2×20 mL). The organic extracts were washed with brine, dried over anhydrous sodium sulfate, filtered and the solvent was removed in vacuo. The residue was purified on silica gel to afford 2-(2-oxo-2-phenyl-ethoxymethyl)-4-(piperidine-1-sulfonyl)-benzonitrile as a pale yellow solid (59 mg, 31%)
1H-NMR (CDCl3) δ: 8.10 (s, 1H), 7.95 (d, 2H), 7.81 (t, 2H), 7.63 (t, 1H), 7.51 (t, 2H), 5.00 (s, 2H), 4.96 (s, 2H), 3.04 (t, 4H), 1.62-1.70 (m, 4H), 1.42-1.47 (m, 2H).
To a mixture of 2-chloro-5-chlorosulfonyl benzoic acid (6.66 g, 21.9 mmol) and CH2Cl2 (18 ml) were added a few drops of DMF and a CH2Cl2 solution of Cl2C2O2 (2 M, 40 ml, 80 mmol) at 0° C. The mixture was stirred at room temperature overnight. The solvent and excess Cl2C2O2 were removed in vacuo. To this carbonyl chloride was added dry THF (30 ml) and cooled to −78° C. followed by addition of a Grignard solution, generated from BrCH2CH2CH(OMe2)2 (3.4 ml, 25 mmol), Mg (1.5 g, 60 mmol) and ThF (40 ml), rt, 2 hs, over 30 minutes. The mixture was allowed to warm to room temperature overnight with stiring. To this resulting mixture was added HCl aq (10%, 13 ml) and stirred at room temperature for 2 hours. The resulting mixture was extracted with CH2Cl2 (2×80 ml) and the combined extract was dried over Na2SO4, filtered. The solution was concentrated under reduced pressure to afford a residue which was purified on silica gel column to give the pure product 4-[2-chloro-5-piperidine-1-sulfonyl)phenyl]-4-oxobutyraldehyde (6.2 g, 91%)
1H NMR (CDCl3) 8:1.46 (m, 2H), 1.64 (m, 4H), 2.95-3.03 (m, 6H), 3.26 (t, 2H), 7.59 (d, 1H), 7.76 (d, 1H), 7.928 (s, 1H), 9.87 (s, 1H).
In a similar fashion the following compound was prepared.
1H NMR (CDCl3) δ: 1.46 (m, 2H), 1.66 (m, 4H), 2.57 (s, 3H), 2.97-3.03 (m, 6H), 3.25 (t, 2H), 7.28 (d, 1H), 7.75 (d, 1H), 8.06 (s, 1H), 9.91 (s, 1H).
To a solution of NaBH4 (114 mg, 3 mmol) in H2O (2 ml) was added slowly a solution of 4-[2-chloro-5-(piperidine-1-sulfonyl)phenyl]-4-oxobutyraldehyde (171 mg, 0.5 mmol) in THF (3 ml) at 0° C. The mixture was stirred at this temperature for 30 minutes and neutralized with diluted HCl aq (1N) followed by extraction with CH2Cl2 (5 ml×3). The combined extract was dried and the solvent was removed under reduced pressure to leave the corresponding diol as a white solid. To the diol was added CH2ClCH2Cl (1.5 ml), Ph2SiHCl (360 μl, 1.68 mmol) and 1 nCl3 (18 mg, 0.08 mmol) and the mixture was heated to 80° C. for 3 hours. The resulting mixture was treated with diluted HF aq (1 N, 1 ml) at room temperature for 30 minutes and extracted with CH2Cl2 (5 ml×3). After normal work-up, purification on silica gel column afforded a mixture containing unsaturated primary monoalcohol which was hydrogenated with H2 (1 atm) in the presence of Pd/C (10%, 15 mg) in EtOH (2 ml) at room temperature for 2 hours to provide pure saturated primary monoalcohol. The alcohol was oxidized with PCC (250 mg, 1 mmol) and CH2Cl2 (5 ml) at room temperature for 2 hours. Purification on silica gel gave the final product (54 mg, 33%) after normal work-up.
1H NMR (CDCl3) δ: 1.45 (m, 2H), 1.67 (m, 4H), 2.00 (m, 2H), 2.55 (t, 2H), 2.85 (t, 2H), 3.00 (t, 4H), 7.50-7.66 (m, 3H), 9.83 (s, 1H).
To a THF solution of aryl bromide (or iodide, 1.15 eqv.) was added n-BuLi (1.6 M, 1.15 eqv.) in hexane at −78° C. The mixture was stirred at this temperature for 1 h. To this was added a solution of 4-[2-chloro-5-(piperidine-1-sulfonyl)-phenyl]-4-oxo-butyraldehyde (1 eqv.) in THF at −78° C. and was kept stirring at this temperature for 2 hours. To this resulting mixture was added H2O (1 ml) at rt. and extracted with AcOEt (3×3 ml). The combined extract was dried and concentrated to afford residue. The residue was treated with PCC (3 eqv.) and CH2Cl2 at room temperature for 2 hours. The resulting solution was taken and was concentrated to give a residue, which was purified on silica gel column to afford the products.
1H NMR (CDCl3) δ: 1.455 (m, 2H), 1.672 (m, 4H), 3.044 (t, J=5.3 Hz, 4H), 3.430 (t, J=6.2 Hz, 2H), 3.655 (t, J=6.2 Hz, 2H), 7.596-8.045 (m, 9H), 8.559 (s, 1H).
1H NMR (CDCl3) δ: 1.437 (m, 2H), 1.639 (m, 4H), 3.045 (t, J=5.4 Hz, 4H), 3.440 (t, J=5.7 Hz, 2H), 3.586 (t, J=5.7 Hz, 2H), 7.515-7.627 (m, 4H), 7.788 (dd, J=2.1 Hz, J=8.2 Hz, 1H), 7.89 (d, J=7.7 Hz, 1H), 8.010-8.073 (m, 3H), 8.603(d, J=9.0 Hz, 1H).
1H NMR (CDCl3) δ: 1.467 (m, 2H), 1.664 (m, 4H), 3.033 (t, J=5.4 Hz, 4H), 3.369 (t, J=5.8 Hz, 2H), 3.511 (t, J=5.8 Hz, 2H), 7.464-7.610 (m, 4H), 7.772 (dd, J=2.1 Hz, J=8.4 Hz, 1H), 8.015(m, 3H).
1H NM (CDCl3) δ: 1.465 (m, 2H), 1.658 (m, 4H), 3.027 (t, J=5.4 Hz, 4H), 3.368-3.522 (m, 4H), 7.455 (m, 1H), 7.602 (d, J=8.4 Hz, 1H), 7.775 (dd, J=2.1 Hz, J=8.4 Hz, 1H), 8.015 (d, J=2.1 Hz, 1H), 8.276 (m, 1H), 8.813 (t, J=2.4 Hz, 1H), 9.238 (d, J=1.8 Hz, 1H).
1H NMR (CDCl3) δ: 1.462 (m, 2H), 1.643 (m, 4H), 2.251 (s, 6H), 3.028 (t, J=5.3 Hz, 4H), 3.335-3.535 (m, 6H), 7.436 (t, J=7.6 Hz, 1H), 7.539-7.605 (m, 2H), 7.765 (dd, J=2.4 Hz, J=8.6 Hz, 1H), 7.897 (d, J=7.6 Hz, 1H), 7.934 (s, 1H), 8.009 (d, J=2.4 Hz, 1H).
1H NMR (CDCl3) δ: 1.45(m, 2H), 1.67 (m, 4H), 2.13 (m, 2H), 2.90-3.28 (m, 8H), 7.28-7.66(m, 6H), 7.96 (d, 2H).
1H NMR (CDCl3) δ: 1.46(m, 2H), 1.68 (m, 4H), 2.153 (m, 2H), 2.91-3.13(m, 8H), 7.47-7.71(m, 4H), 8.24 (d, 1H), 8.83 (broad, 1H), 9.09 (broad, 1H).
The following compounds (Examples 118-120) were prepared in a similar fashion to that stated above with the exception that in the final oxidation step KMnO4, (1 eqiv.), acetone (3 ml) and H2O (1 ml) were used at 0° C. for oxidation.
1H NMR (CDCl3) δ: 1.48 (m, 2H), 1.66 (m, 411), 3.04 (t, 4H), 3.39 (t, 2H), 3.74 (t, 2H), 7.56-8.65 (m, 6H), 8.74 (d, 1H).
1H NMR (CDCl3) δ: 1.45 (m, 2H), 1.67 (m, 4H), 2.56 (s, 3H), 3.02(t, 4H), 3.37 (t, 2H), 3.72 (t, 2H), 7.42-7.53(m, 2H), 7.73-7.89(m, 2H), 8.04 (d, 1H), 8.12 (s, 1H), 8.74 (d, 1H).
1H NMR (CDCl3) δ: 1.45(m, 2H), 1.65(m, 4H), 2.91-3.00 (m, 6H), 3.35 (t, 2H), 7.49-7.53(m, 3H), 7.67 (s, 1H), 7.87 (d, H), 8.05 (d, 1H), 8.71 (d, 1H).
To a solution of (2-chloro-5-(piperidine-1-sulfonyl)-phenyl)methanol (54 mg, 0.18 mmol) in THF (1.5 ml) was added NaH (60% in oil, 20 mg, 0.5 mmol) at 0° C. and kept stirring at this temperature for 40 minutes. To this reaction mixture was added a solution of 3-chloromethyl-5-thiophen-2-yl-(1,2,4) oxadoazole (30 mg, 0.15 mmol) in THF (1 ml) at 0° C. and stirred at room temperature for 150 minutes. To this resulting mixture was added H2O (1 ml) at 0° C. and extracted with CH2Cl2 (5 ml×3). The combined extract was dried and the solvent was removed with rotary evaporator to give residue, which was purified on silica gel column to afford pure product (65 mg, 96%)
1H NMR (CDCl3) δ: 1.45 (m, 2H), 1.65 (m, 4H), 3.01 (t, 4H), 4.85 (s, 4H), 7.50 (m, 2H), 7.66 (d, 1H), 7.72 (m, 1H), 7.99 (s, 1H), 8.30 (d, 1H).
To a solution of 2-chloro-5-(piperidine-1-sulfonyl)-benzaldehyde (86 mg, 0.3 mmol) in THF (2 ml) was added vinylmagnesium bromide (1M, 0.4 ml, 0.4 mmol) in THF at −60° C. and allowed to warm to room temperature over 1 h. To this resulting mixture was added a saturated NH4Cl aq (1 ml) and extracted with CH2Cl2 (5 ml×3). The combined extract was dried and the solvent was removed with rotary evaporator to leave residue. To this was added Cl2SO (118 mg, 1 mmol) and Et2O (3 ml) at 0° C. and stirred at room temperature overnight. After standard work-up, purification on silica gel column afforded a mixture containing regioisomers. The isomers were heated at 110° C. overnight in DMF (1.5 ml) and cooled to room temperature. To this were added KI (50 mg, 0.3 mmol), HSPh (40 mg, 0.36 mmol) and K2CO3 (50 mg, 0.36 mmol) and stirred at room temperature overnight. Standard work-up and purification on silica gel column gave the intermediate sulfide, which was oxidized with 3-chloroperoxybenzoic acid (52 mg, 0.3 nmol) in CH2Cl2 (2 ml) at room temperature overnight. The CH2Cl2 was removed and the residue was purified on silica gel column to provide final product (79 mg, 47%)
1H NMR (CDCl3) 8:1.46 (m, 2H), 1.68 (m, 4H), 3.01 (t, 4H), 3.70-3.84 (m, 2H), 6.10 (m, 1H), 6.85 (d, 1H) 7.28-7.72(m, 7H), 8.09(s, 1H).
A mixture of (125 mg, 0.36 mmol), CH2Cl2 solution of oxalyl chloride (2 M, 0.8 ml, 1.6 mmol) and CH2Cl2 (2.5 ml) was heated to 50° C. for 2 hours. The excess oxalyl chloride and solvent were removed in vacuo to afford solid acid chloride, which was diluted with dry THF (2.5 ml) and cooled to −50° C. To this solution was added a mixture, generated from 1H-pyrrolo[2,3-b]pyridine (85 mg, 0.72 mmol), sodium (hexamethyldisilyl)amide (1 M, 0.6 ml, 0.6 mmol), THF (2 ml) at 0° C. for 30 minutes, at −50° C. The mixture was allowed to warm to room temperature overnight. To this resulting mixture was added H2O (1 ml) and extracted with AcOEt (3×5 ml). The combined extract was dried and the solvent was removed in vacuo to give a residue, which was purified on silica gel column to afford the pure product (50.7 mg, 31%)
1H NMR (CDCl3) δ: 1.431 (m, 2H), 1.656 (m, 4H), 3.027 (t, J=5.6 Hz, 4H), 4.971 (s, 2H), 5.447 (s, 2H), 6.688 (d, J=4.2 Hz, 1H), 7.258 (m, 1H), 7.535 (d, J=8.1 Hz, 1H), 7.663 (dd, J=2.1 Hz, J=8.1 Hz, 1H), 7.920 (dd, J=2.1 Hz, J=8.1 Hz, 1H), 8.030 (d, J=3.9 Hz, 1H), 8.082 (s, 1H), 8.365 (d, J=5.1 Hz, 1H).
To a mixture of the N-Boc-glycine (350 mg, 2 mmol), and HOBt (297 mg, 2.2 mmol) in DMF(10 mL) was added piperidine (187 mg, 2.2 mmol) and EDCI (422 mg, 2.2 mmol) at 0° C. The mixture was allowed to warm slowly to room temperature overnight (18 hrs). The mixture was diluted with ethyl acetate (300 mL) and washed with H2O (3×250 mL) followed by brine. The organic extract was dried over Na2SO4 (anhydrous) and concentrated in vacuo giving a product which pure enough to use in the next step.
To the above product, dissolved in CH2Cl2 (5 mL) at 0° C., was added slowly trifluoroacetic acid (5 mL). The mixture was stirred at this temperature for 1 hr and then poured into cold NaHCO3 (saturated) and the neutralized mixture was then extracted with CH2Cl2. The organic extract was washed with H2O (3×250 mL) followed by brine. The organic extract was dried over Na2SO4 (anhydrous) and concentrated in vacuo and the residue was purified by silica gel flash chromatography giving the 2-amino-1-piperidin-1-ylethanone as a colourless oil (82 mg, 29% overall yield).
To a solution of the 2-chloro-5-(piperidine-1-sulfonyl)benzaldehyde and 2-amino-1-piperidin-1-ylethanone in dichloroethane at room temperature was added NaBH(OAc)3 and the mixture was allowed to stir at room temperature overnight. The mixture was then diluted with ethyl acetate and the washed with NaHCO3 (saturated). The organic layer was then washed with brine and then dried over Na2SO4 (anhydrous) and the organic solvent was removed in vacuo. The residue was purified by flash chromatography on silica gel using hexane/ethyl acetate (0-100%) as eluant giving 62 mg (62% yield) of product as a colourless oil
1H-NMR (CDCl3) δ: 7.90 (d, 1H), 7.59 (dd, 1H), 7.51 (d, 1H), 3.98 (s, 2H), 3.58 (t, 2H), 3.46 (s, 2H), 3.30 (t, 2H), 3.00 (t, 4H), 2.06 (br, 1H), 1.69-1.50 (m, 1H), 1.42 (m, 2H).
The foregoing examples are meant to illustrate the invention and are not to be construed to limit the invention in any way. Those skilled in the art will recognize modifications that are within the spirit and scope of the invention.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/US04/11544 | 4/15/2004 | WO | 7/17/2006 |
| Number | Date | Country | |
|---|---|---|---|
| 60462697 | Apr 2003 | US |
