TREA

Therapeutic polypeptides, nucleic acids encoding same, and methods of use

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Information

  • Patent Application
  • 20060003323
  • Publication Number
    20060003323
  • Date Filed
    June 03, 2003
    21 years ago
  • Date Published
    January 05, 2006
    18 years ago
Information
Abstract
Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies that immunospecifically bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the novel polypeptide, polynucleotide, or antibody specific to the polypeptide. Vectors, host cells, antibodies and recombinant methods for producing the polypeptides and polynucleotides, as well as methods for using same are also included. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.
Description
FIELD OF THE INVENTION

The present invention relates to novel polypeptides, and the nucleic acids encoding them, having properties related to stimulation of biochemical or physiological responses in a cell, a tissue, an organ or an organism. More particularly, the novel polypeptides are gene products of novel genes, or are specified biologically active fragments or derivatives thereof. Methods of use encompass diagnostic and prognostic assay procedures as well as methods of treating diverse pathological conditions.


BACKGROUND OF THE INVENTION

Eukaryotic cells are characterized by biochemical and physiological processes which under normal conditions are exquisitely balanced to achieve the preservation and propagation of the cells. When such cells are components of multicellular organisms such as vertebrates, or more particularly organisms such as mammals, the regulation of the biochemical and physiological processes involves intricate signaling pathways. Frequently, such signaling pathways involve extracellular signaling proteins, cellular receptors that bind the signaling proteins, and signal transducing components located within the cells.


Signaling proteins may be classified as endocrine effectors, paracrine effectors or autocrine effectors. Endocrine effectors are signaling molecules secreted by a given organ into the circulatory system, which are then transported to a distant target organ or tissue. The target cells include the receptors for the endocrine effector, and when the endocrine effector binds, a signaling cascade is induced. Paracrine effectors involve secreting cells and receptor cells in close proximity to each other, for example two different classes of cells in the same tissue or organ. One class of cells secretes the paracrine effector, which then reaches the second class of cells, for example by diffusion through the extracellular fluid. The second class of cells contains the receptors for the paracrine effector; binding of the effector results in induction of the signaling cascade that elicits the corresponding biochemical or physiological effect. Autocrine effectors are highly analogous to paracrine effectors, except that the same cell type that secretes the autocrine effector also contains the receptor. Thus the autocrine effector binds to receptors on the same cell, or on identical neighboring cells. The binding process then elicits the characteristic biochemical or physiological effect.


Signaling processes may elicit a variety of effects on cells and tissues including by way of nonlimiting example induction of cell or tissue proliferation, suppression of growth or proliferation, induction of differentiation or maturation of a cell or tissue, and suppression of differentiation or maturation of a cell or tissue.


Many pathological conditions involve dysregulation of expression of important effector proteins. In certain classes of pathologies the dysregulation is manifested as diminished or suppressed level of synthesis and secretion of protein effectors. In other classes of pathologies the dysregulation is manifested as increased or up-regulated level of synthesis and secretion of protein effectors. In a clinical setting a subject may be suspected of suffering from a condition brought on by altered or mis-regulated levels of a protein effector of interest. Therefore there is a need to assay for the level of the protein effector of interest in a biological sample from such a subject, and to compare the level with that characteristic of a nonpathological condition. There also is a need to provide the protein effector as a product of manufacture. Administration of the effector to a subject in need thereof is useful in treatment of the pathological condition. Accordingly, there is a need for a method of treatment of a pathological condition brought on by a diminished or suppressed levels of the protein effector of interest. In addition, there is a need for a method of treatment of a pathological condition brought on by a increased or up-regulated levels of the protein effector of interest.


Antibodies are multichain proteins that bind specifically to a given antigen, and bind poorly, or not at all, to substances deemed not to be cognate antigens. Antibodies are comprised of two short chains termed light chains and two long chains termed heavy chains. These chains are constituted of immunoglobulin domains, of which generally there are two classes: one variable domain per chain, one constant domain in light chains, and three or more constant domains in heavy chains. The antigen-specific portion of the immunoglobulin molecules resides in the variable domains; the variable domains of one light chain and one heavy chain associate with each other to generate the antigen-binding moiety. Antibodies that bind immunospecifically to a cognate or target antigen bind with high affinities. Accordingly, they are useful in assaying specifically for the presence of the antigen in a sample. In addition, they have the potential of inactivating the activity of the antigen.


Therefore there is a need to assay for the level of a protein effector of interest in a biological sample from such a subject, and to compare this level with that characteristic of a nonpathological condition. In particular, there is a need for such an assay based on the use of an antibody that binds immunospecifically to the antigen. There further is a need to inhibit the activity of the protein effector in cases where a pathological condition arises from elevated or excessive levels of the effector based on the use of an antibody that binds immunospecifically to the effector. Thus, there is a need for the antibody as a product of manufacture. There further is a need for a method of treatment of a pathological condition brought on by an elevated or excessive level of the protein effector of interest based on administering the antibody to the subject.


SUMMARY OF THE INVENTION

The invention is based in part upon the discovery of isolated polypeptides including amino acid sequences selected from mature forms of the amino acid sequences selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606. The novel nucleic acids and polypeptides are referred to herein as NOV1a, NOV1b, NOV1c, NOV1d, NOV2a, NOV2b, NOV2c, NOV2d, NOV3a, NOV3b, etc. These nucleic acids and polypeptides, as well as derivatives, homologs, analogs and fragments thereof, will hereinafter be collectively designated as “NOVX” nucleic acid or polypeptide sequences.


The invention also is based in part upon variants of a mature form of the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, wherein any amino acid in the mature form is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence of the mature form are so changed. In another embodiment, the invention includes the amino acid sequences selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606. In another embodiment, the invention also comprises variants of the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 wherein any amino acid specified in the chosen sequence is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence are so changed. The invention also involves fragments of any of the mature forms of the amino acid sequences selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, or any other amino acid sequence selected from this group. The invention also comprises fragments from these groups in which up to 15% of the residues are changed.


In another embodiment, the invention encompasses polypeptides that are naturally occurring allelic variants of the sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606. These allelic variants include amino acid sequences that are the translations of nucleic acid sequences differing by a single nucleotide from nucleic acid sequences selected from the group consisting of SEQ ID NOS: 2n−1, wherein n is an integer between 1 and 606. The variant polypeptide where any amino acid changed in the chosen sequence is changed to provide a conservative substitution.


In another embodiment, the invention comprises a pharmaceutical composition involving a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 and a pharmaceutically acceptable carrier. In another embodiment, the invention involves a kit, including, in one or more containers, this pharmaceutical composition.


In another embodiment, the invention includes the use of a therapeutic in the manufacture of a medicament for treating a syndrome associated with a human disease, the disease being selected from a pathology associated with a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 wherein said therapeutic is the polypeptide selected from this group.


In another embodiment, the invention comprises a method for determining the presence or amount of a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 in a sample, the method involving providing the sample; introducing the sample to an antibody that binds immunospecifically to the polypeptide; and determining the presence or amount of antibody bound to the polypeptide, thereby determining the presence or amount of polypeptide in the sample.


In another embodiment, the invention includes a method for determining the presence of or predisposition to a disease associated with altered levels of a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 in a first mammalian subject, the method involving measuring the level of expression of the polypeptide in a sample from the first mammalian subject; and comparing the amount of the polypeptide in this sample to the amount of the polypeptide present in a control sample from a second mammalian subject known not to have, or not to be predisposed to, the disease, wherein an alteration in the expression level of the polypeptide in the first subject as compared to the control sample indicates the presence of or predisposition to the disease.


In another embodiment, the invention involves a method of identifying an agent that binds to a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, the method including introducing the polypeptide to the agent; and determining whether the agent binds to the polypeptide. The agent could be a cellular receptor or a downstream effector.


In another embodiment, the invention involves a method for identifying a potential therapeutic agent for use in treatment of a pathology, wherein the pathology is related to aberrant expression or aberrant physiological interactions of a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, the method including providing a cell expressing the polypeptide of the invention and having a property or function ascribable to the polypeptide; contacting the cell with a composition comprising a candidate substance; and determining whether the substance alters the property or function ascribable to the polypeptide; whereby, if an alteration observed in the presence of the substance is not observed when the cell is contacted with a composition devoid of the substance, the substance is identified as a potential therapeutic agent.


In another embodiment, the invention involves a method forscreening for a modulator of activity or of latency or predisposition to a pathology associated with a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, the method including administering a test compound to a test animal at increased risk for a pathology associated with the polypeptide of the invention, wherein the test animal recombinantly expresses the polypeptide of the invention; measuring the activity of the polypeptide in the test animal after administering the test compound; and comparing the activity of the protein in the test animal with the activity of the polypeptide in a control animal not administered the polypeptide, wherein a change in the activity of the polypeptide in the test animal relative to the control animal indicates the test compound is a modulator of latency of, or predisposition to,. a pathology associated with the polypeptide of the invention. The recombinant test animal could express a test protein transgene or express the transgene under the control of a promoter at an increased level relative to a wild-type test animal The promoter may or may not b the native gene promoter of the transgene.


In another embodiment, the invention involves a method for modulating the activity of a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, the method including introducing a cell sample expressing the polypeptide with a compound that binds to the polypeptide in an amount sufficient to modulate the activity of the polypeptide.


In another embodiment, the invention involves a method of treating or preventing a pathology associated with a polypeptide with an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, the method including administering the polypeptide to a subject in which such treatment or prevention is desired in an amount sufficient to treat or prevent the pathology in the subject. The subject could be human.


In another embodiment, the invention involves a method of treating a pathological state in a mammal, the method including administering to the mammal a polypeptide in an amount that is sufficient to alleviate the. pathological state, wherein the polypeptide is a polypeptide having an amino acid sequence at least 95% identical to a polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 or a biologically active fragment thereof.


In another embodiment, the invention involves an isolated nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide having an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606; a variant of a mature form of the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 wherein any amino acid in the mature form of the chosen sequence is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence of the mature form are so changed; the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606; a variant of the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606, in which any amino acid specified in the chosen sequence is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence are so changed; a nucleic acid fragment encoding at least a portion of a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 or any variant of the polypeptide wherein any amino-acid of the chosen sequence is changed to a different amino acid, provided that no more than 10% of the amino acid residues in the sequence are so changed; and the complement of any of the nucleic acid molecules.


In another embodiment, the invention comprises an isolated nucleic acid molecule having a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606, wherein the nucleic acid molecule comprises the nucleotide sequence of a naturally occurring allelic nucleic acid variant.


In another embodiment, the invention involves an isolated nucleic acid, molecule including a nucleic acid sequence encoding a polypeptide having an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606 that encodes a variant polypeptide, wherein the variant polypeptide has the polypeptide sequence of a naturally occurring polypeptide variant.


In another embodiment, the invention comprises an isolated nucleic acid molecule having a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606, wherein the nucleic acid molecule differs by a single nucleotide from a nucleic acid sequence selected from the group consisting of SEQ. ID NOS: 2n−1, wherein n is an integer between 1 and 606.


In another embodiment, the invention includes an isolated nucleic acid molecule having a nucleic acid sequence encoding a polypeptide including an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606, wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of the nucleotide sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606; a nucleotide sequence wherein one or more nucleotides in the nucleotide sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606 is changed from that selected from the group consisting of the chosen sequence to a different nucleotide provided that no more than 15% of the nucleotides are so changed; a nucleic acid fragment of the sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606; and a nucleic acid fragment wherein one or more nucleotides in the nucleotide sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606 is changed from that selected from the group consisting of the chosen sequence to a different nucleotide provided that no more than 15% of the nucleotides are so changed.


In another embodiment, the invention includes an isolated nucleic acid molecule having a nucleic acid sequence encoding a polypeptide including an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n isan integer between 1 and 606, wherein the nucleic acid molecule hybridizes under stringent conditions to the nucleotide sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or a complement of the nucleotide sequence.


In another embodiment, the invention includes an isolated nucleic acid molecule having a nucleic acid sequence encoding. a polypeptide including an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606, wherein the nucleic acid molecule has a nucleotide sequence in which any nucleotide specified in the coding sequence of the chosen nucleotide sequence is changed from that selected from the group consisting of the chosen sequence to a different nucleotide provided that no more than 15% of the nucleotides in the chosen coding sequence are so changed, an isolated second polynucleotide that is a complement of the first polynucleotide, or a fragment of any of them.


In another embodiment, the invention includes a vector involving the nucleic acid molecule having a nucleic acid sequence encoding a polypeptide including an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606. This vector can have a promoter operably linked to the nucleic acid molecule. This vector can be located within a cell.


In another embodiment, the invention involves a method for determining the presence or amount of a nucleic acid molecule having a nucleic acid sequence encoding a polypeptide including an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606 in a sample, the method including providing the sample; introducing the sample to a probe that binds to the nucleic acid molecule; and determining the presence or amount of the probe bound to the nucleic acid molecule, thereby determining the presence or amount of the nucleic acid molecule in the sample. The presence or amount of the nucleic acid molecule is used as a marker for cell or tissue type. The cell type can be cancerous.


In another embodiment, the invention involves a method for determining the presence of or predisposition for a disease associated with altered levels of a nucleic acid molecule having a nucleic acid sequence encoding a polypeptide including an amino acid sequence selected from the group consisting of a mature form of the amino acid sequence given SEQ ID NO:2n, wherein n is an integer between 1 and 606 in a first mammalian subject, the method including measuring the amount of the nucleic acid in a sample from the first mammalian subject; and comparing the amount of the nucleic acid in the sample of step (a) to the amount of the nucleic acid present in a control sample from a second mammalian subject known not to have or not be predisposed to, the disease; wherein an alteration in the level of the nucleic acid in the first subject as compared to the control sample indicates the presence of or predisposition to the disease.


The invention further provides an antibody that binds immunospecifically to a NOVX polypeptide. The NOVX antibody may be monoclonal, humanized, or a fully human antibody. Preferably, the antibody has a dissociation constant for the binding of the NOVX polypeptide to the antibody less than 1×10−9 M. More preferably, the NOVX antibody neutralizes the activity of the NOVX polypeptide.


In a further aspect, the invention provides for the use of a therapeutic in the manufacture of a medicament for treating a syndrome associated with a human disease, associated with a NOVX polypeptide. Preferably the therapeutic is a NOVX antibody.


In yet a further aspect, the invention provides a method of treating or preventing a NOVX-associated disorder, a method of treating a pathological state in a mammal, and a method of treating or preventing a pathology associated with a polypeptide by administering a NOVX antibody to a subject in an amount sufficient to treat or prevent the disorder.


Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be limiting.


Other features and advantages of the invention will be apparent from the following detailed description and claims.




BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 is a table illustrating the SAGE library data results illustrating overexpression of LIV-1 in breast carcinoma derived cells.



FIG. 2 is is a bar graph showing that antisense knockdown of LIV-1 inhibits breast carcinoma cell line T47D cell growth.



FIG. 3 is is a bar graph showing that antisense knockdown of LwV-1 has less inhibitory effect on Ovcar-4 cell growth.



FIG. 4 is is a bar graph showing RTQPCR validation of knockdown of LIV-1 expression at mRNA level in T47D breast cancer cell lines.




DETAILED DESCRIPTION OF THE INVENTION

The present invention provides novel nucleotides and polypeptides encoded thereby. Included in the invention are the novel nucleic acid sequences, their encoded polypeptides, antibodies, and other related compounds. The sequences are collectively referred to herein as “NOVX nucleic acids” or “NOVX polynucleotides” and the corresponding encoded polypeptides are referred to as “NOVX polypeptides” or “NOVX proteins.” Unless indicated otherwise, “NOVX” is meant to refer to any of the novel sequences disclosed herein. Table A provides a summary of the NOVX nucleic acids and their encoded polypeptides.

TABLE ASequences and Corresponding SEQ ID NumbersNOVXInternalSEQ ID NOSEQ ID NOAssignmentIdentification(nucleic acid)(amino acid)HomologyNOV1aCG191083-0112VON WILLEBRAND factor A- related protein homolog - Mus musculusNOV2aCG191745-0134Integrin alpha-2 precursor (Plateletmembrane glycoprotein Ia) (GPIa) (CD49b) - Homo sapiensNOV3aCG50253-0156Human secreted protein SCEP-39NOV3b25009576578Human secreted protein SCEP-39NOV3c250095742910Human secreted protein SCEP-39NOV3d2500957791112Human secreted protein SCEP-39NOV3e2500957941314Human secreted protein SCEP-39NOV3f2500957341516Human secreted protein SCEP-39NOV3g2500958111718Human secreted protein SCEP-39NOV3h2500957991920Human secreted protein SCEP-39NOV3iSNP133776092122Human secreted protein SCEP-39NOV3jSNP133739292324Human secreted protein SCEP-39NOV3kSNP133802722526Human secreted protein SCEP-39NOV3lSNP133797452728Human secreted protein SCEP-39NOV3mSNP133739302930Human secreted protein SCEP-39NOV4aCG50377-043132CSMD1 - Mus musculusNOV4bCG50377-013334CSMD1 - Mus musculusNOV4cCG50377-023536CSMD1 - Mus musculusNOV4dCG50377-033738CSMD1 - Mus musculusNOV4eCG50377-053940CSMD1 - Mus musculusNOV4fCG50377-064142CSMD1 - Mus musculusNOV4g2730951474344CSMD1 - Mus musculusNOV4h3174596534546CSMD1 - Mus musculusNOV4i3174596124748CSMD1 - Mus musculusNOV4j3172863314950CSMD1 - Mus musculusNOV4kCG50377-075152CSMD1 - Mus musculusNOV4lCG50377-085354CSMD1 - Mus musculusNOV4mCG50377-095556CSMD1 - Mus musculusNOV4nCG50377-105758CSMD1 - Mus musculusNOV4oCG50377-115960CSMD1 - Mus musculusNOV4pSNP133824576162CSMD1 - Mus musculusNOV4qSNP133824586364CSMD1 - Mus musculusNOV5aCG50389-046566Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5bCG50389-066768Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5c2574486486970Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5dCG50389-017172Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5eCG50389-027374Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5fCG50389-037576Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5gCG50389-057778Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV5hSNP133824647980Interleukin 1 receptor-like 2 precursor (IL-1Rrp2)(IL1R-rp2) - Homo sapiensNOV6aCG50391-088182Human protease-inhibitor like proteinNOV6bCG50391-098384Human protease-inhibitor like proteinNOV6cCG50391-108586Human protease-inhibitor like proteinNOV6dCG50391-018788Human protease-inhibitor like proteinNOV6eCG50391-028990Human protease-inhibitor like proteinNOV6fCG50391-039192Human protease-inhibitor like proteinNOV6gCG50391-049394Human protease-inhibitor like proteinNOV6hCG50391-059596Human protease-inhibitor like proteinNOV6iCG50391-069798Human protease-inhibitor like proteinNOV6jCG50391-0799100Human protease-inhibitor like proteinNOV6kCG50391-11101102Human protease-inhibitor like proteinNOV6lSNP13376336103104Human protease-inhibitor like proteinNOV6mSNP13376335105106Human protease-inhibitor like proteinNOV6nSNP13376334107108Human protease-inhibitor like proteinNOV6oSNP13376333109110Human protease-inhibitor like proteinNOV6pSNP13382488111112Human protease-inhibitor like proteinNOV7aCG50426-17113114Ten-m2 - Mus musculusNOV7bCG50426-21115116Ten-m2 - Mus musculusNOV7cCG50426-15117118Ten-m2 - Mus musculusNOV7d306276924119120Ten-m2 - Mus musculusNOV7e306276936121122Ten-m2 - Mus musculusNOV7f308530526123124Ten-m2 - Mus musculusNOV7g308530589125126Ten-m2 - Mus musculusNOV7hCG50426-01127128Ten-m2 - Mus musculusNOV7iCG50426-02129130Ten-m2 - Mus musculusNOV7jCG50426-03131132Ten-m2 - Mus musculusNOV7kCG50426-04133134Ten-m2 - Mus musculusNOV7lCG50426-05135136Ten-m2 - Mus musculusNOV7mCG50426-06137138Ten-m2 - Mus musculusNOV7nCG50426-07139140Ten-m2 - Mus musculusNOV7oCG50426-08141142Ten-m2 - Mus musculusNOV7pCG50426-09143144Ten-m2 - Mus musculusNOV7qCG50426-10145146Ten-m2 - Mus musculusNOV7rCG50426-11147148Ten-m2 - Mus musculusNOV7sCG50426-12149150Ten-m2 - Mus musculusNOV7tCG50426-13151152Ten-m2 - Mus musculusNOV7uCG50426-14153154Ten-m2 - Mus musculusNOV7vCG50426-16155156Ten-m2 - Mus musculusNOV7wCG50426-18157158Ten-m2 - Mus musculusNOV7xCG50426-19159160Ten-m2 - Mus musculusNOV7yCG50426-20161162Ten-m2 - Mus musculusNOV7zCG50426-22163164Ten-m2 - Mus musculusNOV7aCG50426-23165166Ten-m2 - Mus musculusNOV7bCG50426-24167168Ten-m2 - Mus musculusNOV7cCG50426-25169170Ten-m2 - Mus musculusNOV7dSNP13375665171172Ten-m2 - Mus musculusNOV7eSNP13375213173174Ten-m2 - Mus musculusNOV7fSNP13374985175176Ten-m2 - Mus musculusNOV8aCG50646-04177178Polydom protein precursor - Mus musculusNOV8b237582568179180Polydom protein precursor - Mus musculusNOV8c236589434181182Polydom protein precursor - Mus musculusNOV8d236495259183184Polydom protein precursor - Mus musculusNOV8e236495256185186Polydom protein precursor - Mus musculusNOV8fCG50646-01187188Polydom protein precursor - Mus musculusNOV8gCG50646-02189190Polydom protein precursor - Mus musculusNOV8hCG50646-03191192Polydom protein precursor - Mus musculusNOV8iCG50646-05193194Polydom protein precursor - Mus musculusNOV8jSNP13380898195196Polydom protein precursor - Mus musculusNOV8kSNP13380899197198Polydom protein precursor - Mus musculusNOV8lSNP13374702199200Polydom protein precursor - Mus musculusNOV8mSNP13374257201202Polydom protein precursor - Mus musculusNOV8nSNP13382479203204Polydom protein precursor - Mus musculusNOV8oSNP13382480205206Polydom protein precursor - Mus musculusNOV9aCG50736-09207208CD44-like precursor FELL - Homo sapiensNOV9b197408749209210CD44-like precursor FELL - Homo sapiensNOV9cCG50736-01211212CD44-like precursor FELL - Homo sapiensNOV9dCG50736-02213214CD44-like precursor FELL - Homo sapiensNOV9eCG50736-03215216CD44-like precursor FELL - Homo sapiensNOV9fCG50736-04217218CD44-like precursor FELL - Homo sapiensNOV9gCG50736-05219220CD44-like precursor FELL - Homo sapiensNOV9hCG50736-06221222CD44-like precursor FELL - Homo sapiensNOV9iCG50736-07223224CD44-like precursor FELL - Homo sapiensNOV9jCG50736-08225226CD44-like precursor FELL - Homo sapiensNOV9kCG50736-10227228CD44-like precursor FELL - Homo sapiensNOV9lCG50736-11229230CD44-like precursor FELL - Homo sapiensNOV9mCG50736-12231232CD44-like precursor FELL - Homo sapiensNOV9nCG50736-13233234CD44-like precursor FELL - Homo sapiensNOV9oCG50736-14235236CD44-like precursor FELL - Homo sapiensNOV9pCG50736-15237238CD44-like precursor FELL - Homo sapiensNOV9qCG50736-16239240CD44-like precursor FELL - Homo sapiensNOV9rCG50736-17241242CD44-like precursor FELL - Homo sapiensNOV9sCG50736-18243244CD44-like precursor FELL - Homo sapiensNOV9tCG50736-19245246CD44-like precursor FELL - Homo sapiensNOV9uCG50736-20247248CD44-like precursor FELL - Homo sapiensNOV9vCG50736-21249250CD44-like precursor FELL - Homo sapiensNOV9wCG50736-22251252CD44-like precursor FELL - Homo sapiensNOV9xCG50736-23253254CD44-like precursor FELL - Homo sapiensNOV9yCG50736-24255256CD44-like precursor FELL - Homo sapiensNOV10aCG50925-01257258Tumor endothelial marker 5 precursor - Homo sapiensNOV10bCG50925-08259260Tumor endothelial marker 5 precursor - Homo sapiensNOV10c226990087261262Tumor endothelial marker 5 precursor - Homo sapiensNOV10d320054488263264Tumor endothelial marker 5 precursor - Homo sapiensNOV10eCG50925-02265266Tumor endothelial marker 5 precursor - Homo sapiensNOV10f248208844267268Tumor endothelial marker 5 precursor - Homo sapiensNOV10gCG50925-03269270Tumor endothelial marker 5 precursor - Homo sapiensNOV10hCG50925-04271272Tumor endothelial marker 5 precursor - Homo sapiensNOV10iCG50925-05273274Tumor endothelial marker 5 precursor - Homo sapiensNOV10jCG50925-06275276Tumor endothelial marker 5 precursor - Homo sapiensNOV10kCG50925-07277278Tumor endothelial marker 5 precursor - Homo sapiensNOV10lSNP13380040279280Tumor endothelial marker 5 precursor - Homo sapiensNOV11aCG51027-06281282Human immunoglobulin superfamily protein IGSFP-9NOV11b278391205283284Human immunoglobulin superfamily protein IGSFP-9NOV11c278391050285286Human immunoglobulin superfamily protein IGSFP-9NOV11d277582210287288Human immunoglobulin superfamily protein IGSFP-9NOV11e277582173289290Human immunoglobulin superfamily protein IGSFP-9NOV11fCG51027-09291292Human immunoglobulin superfamily protein IGSFP-9NOV11g316763704293294Human immunoglobulin superfamily protein IGSFP-9NOV11hCG51027-01295296Human immunoglobulin superfamily protein IGSFP-9NOV11iCG51027-02297298Human immunoglobulin superfamily protein IGSFP-9NOV11jCG51027-03299300Human immunoglobulin superfamily protein IGSFP-9NOV11kCG51027-04301302Human immunoglobulin superfamily protein IGSFP-9NOV11lCG51027-05303304Human immunoglobulin superfamily protein IGSFP-9NOV11mCG51027-07305306Human immunoglobulin superfamily protein IGSFP-9NOV11nCG51027-08307308Human immunoglobulin superfamily protein IGSFP-9NOV11oCG51027-10309310Human immunoglobulin superfamily protein IGSFP-9NOV11pCG51027-11311312Human immunoglobulin superfamily protein IGSFP-9NOV11qCG51027-12313314Human immunoglobulin superfamily protein IGSFP-9NOV11rSNP13376340315316Human immunoglobulin superfamily protein IGSFP-9NOV11sSNP13376341317318Human immunoglobulin superfamily protein IGSFP-9NOV12aCG51373-11319320Human G protein-coupled receptorNOV12bCG51373-10321322Human G protein-coupled receptorNOV12cCG51373-08323324Human G protein-coupled receptorNOV12d237582097325326Human G protein-coupled receptorNOV12e294385961327328Human G protein-coupled receptorNOV12f294398021329330Human G protein-coupled receptorNOV12gCG51373-01331332Human G protein-coupled receptorNOV12hCG51373-02333334Human G protein-coupled receptorNOV12iCG51373-03335336Human G protein-coupled receptorNOV12jCG51373-04337338Human G protein-coupled receptorNOV12kCG51373-05339340Human G protein-coupled receptorNOV12lCG51373-06341342Human G protein-coupled receptorNOV12mCG51373-07343344Human G protein-coupled receptorNOV12nCG51373-09345346Human G protein-coupled receptorNOV12oCG51373-12347348Human G protein-coupled receptorNOV12pCG51373-13349350Human G protein-coupled receptorNOV12qCG51373-14351352Human G protein-coupled receptorNOV12rSNP13377352353354Human G protein-coupled receptorNOV12sSNP13379746355356Human G protein-coupled receptorNOV12tSNP13374859357358Human G protein-coupled receptorNOV12uSNP13374858359360Human G protein-coupled receptorNOV12vSNP13374856361362Human G protein-coupled receptorNOV12wSNP13374855363364Human G protein-coupled receptorNOV12xSNP13374854365366Human G protein-coupled receptorNOV12ySNP13374853367368Human G protein-coupled receptorNOV13aCG51622-04369370Von Ebner minor protein - Homo sapiensNOV13bCG51622-03371372Von Ebner minor protein - Homo sapiensNOV13cCG51622-05373374Von Ebner minor protein - Homo sapiensNOV13dCG51622-01375376Von Ebner minor protein - Homo sapiensNOV13e275698370377378Von Ebner minor protein - Homo sapiensNOV13f274054187379380Von Ebner minor protein - Homo sapiensNOV13gCG51622-02381382Von Ebner minor protein - Homo sapiensNOV13hCG51622-06383384Von Ebner minor protein - Homo sapiensNOV13iCG51622-07385386Von Ebner minor protein - Homo sapiensNOV13jCG51622-08387388Von Ebner minor protein - Homo sapiensNOV13kCG51622-09389390Von Ebner minor protein - Homo sapiensNOV13lCG51622-10391392Von Ebner minor protein - Homo sapiensNOV13mSNP13375774393394Von Ebner minor protein - Homo sapiensNOV13nSNP13375775395396Von Ebner minor protein - Homo sapiensNOV14aCG51821-01397398Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14b229823091399400Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14c229823107401402Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14d229823095403404Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14eCG51821-02405406Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14fCG51821-03407408Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14gCG51821-04409410Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14hSNP13382490411412Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14iSNP13382489413414Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14jSNP13377616415416Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV14kSNP13377617417418Sialoadhesin precursor (Sialic acid binding Ig-like lectin-1)(Siglec- 1) (CD169 antigen) - Homo sapiensNOV15aCG51992-05419420CTCL tumor antigen se57-1 - Homo sapiensNOV15bCG51992-01421422CTCL tumor antigen se57-1 - Homo sapiensNOV15cCG51992-02423424CTCL tumor antigen se57-1 - Homo sapiensNOV15dCG51992-03425426CTCL tumor antigen se57-1 - Homo sapiensNOV15eCG51992-04427428CTCL tumor antigen se57-1 - Homo sapiensNOV15fSNP13382486429430CTCL tumor antigen se57-1 - Homo sapiensNOV15gSNP13382487431432CTCL tumor antigen se57-1 - Homo sapiensNOV16aCG52171-04433434Hematopoietic PBX-interacting protein - Homo sapiensNOV16bCG52171-01435436Hematopoietic PBX-interacting protein - Homo sapiensNOV16cCG52171-02437438Hematopoietic PBX-interacting protein - Homo sapiensNOV16dCG52171-03439440Hematopoietic PBX-interacting protein - Homo sapiensNOV16eCG52171-05441442Hematopoietic PBX-interacting protein - Homo sapiensNOV16fCG52171-06443444Hematopoietic PBX-interacting protein - Homo sapiensNOV16gSNP13374628445446Hematopoietic PBX-interacting protein - Homo sapiensNOV16hSNP13377329447448Hematopoietic PBX-interacting protein - Homo sapiensNOV16iSNP13374627449450Hematopoietic PBX-interacting protein - Homo sapiensNOV16jSNP13374626451452Hematopoietic PBX-interacting protein - Homo sapiensNOV16kSNP13374625453454Hematopoietic PBX-interacting protein - Homo sapiensNOV16lSNP13374624455456Hematopoietic PBX-interacting protein - Homo sapiensNOV17aCG52534-06457458Transferrin receptor protein 2 (TfR2) - Homo sapiensNOV17bCG52534-01459460Transferrin receptor protein 2 (TfR2) - Homo sapiensNOV17cCG52534-02461462Transferrin receptor protein 2 (TfR2) - Homo sapiensNOV17dCG52534-03463464Transferrin receptor protein 2 (TfR2) - Homo sapiensNOV17eCG52534-04465466Transferrin receptor protein 2 (TfR2) - Homo sapiensNOV17fCG52534-05467468Transferrin receptor protein 2 (TfR2) - Homo sapiensNOV18aCG52979-03469470G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV18bCG52979-01471472G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV18cCG52979-02473474G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19aCG52988-02475476G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19bCG52988-03477478G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19cCG52988-04479480G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19dCG52988-06481482G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19eCG52988-07483484G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19fCG52988-01485486G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV19gCG52988-05487488G antigen family C 1 protein (Prostate-associated gene protein4) (PAGE-4) (PAGE-1) (JM27) (GAGE-9) - Homo sapiensNOV20aCG53449-04489490Butyrophilin like receptor - Homo sapiensNOV20bCG53449-03491492Butyrophilin like receptor - Homo sapiensNOV20cCG53449-01493494Butyrophilin like receptor - Homo sapiensNOV20dCG53449-02495496Butyrophilin like receptor - Homo sapiensNOV20eCG53449-05497498Butyrophilin like receptor - Homo sapiensNOV20fCG53449-06499500Butyrophilin like receptor - Homo sapiensNOV20gSNP13382434501502Butyrophilin like receptor - Homo sapiensNOV20hSNP13382441503504Butyrophilin like receptor - Homo sapiensNOV21aCG53908-01505506Netrin receptor Unc5h1 - Mus musculusNOV21bCG53908-02507508Netrin receptor Unc5h1 - Mus musculusNOV21c306075989509510Netrin receptor Unc5h1 - Mus musculusNOV21dCG53908-03511512Netrin receptor Unc5h1 - Mus musculusNOV21eCG53908-04513514Netrin receptor Unc5h1 - Mus musculusNOV21fCG53908-05515516Netrin receptor Unc5h1 - Mus musculusNOV21gCG53908-06517518Netrin receptor Unc5h1 - Mus musculusNOV21hCG53908-07519520Netrin receptor Unc5h1 - Mus musculusNOV21iSNP13382444521522Netrin receptor Unc5h1 - Mus musculusNOV21jSNP13375491523524Netrin receptor Unc5h1 - Mus musculusNOV21kSNP13375492525526Netrin receptor Unc5h1 - Mus musculusNOV21lSNP13375493527528Netrin receptor Unc5h1 - Mus musculusNOV21mSNP13375135529530Netrin receptor Unc5h1 - Mus musculusNOV21nSNP13375136531532Netrin receptor Unc5h1 - Mus musculusNOV21oSNP13375137533534Netrin receptor Unc5h1 - Mus musculusNOV21pSNP13375138535536Netrin receptor Unc5h1 - Mus musculusNOV21qSNP13375494537538Netrin receptor Unc5h1 - Mus musculusNOV21rSNP13375495539540Netrin receptor Unc5h1 - Mus musculusNOV21sSNP13375140541542Netrin receptor Unc5h1 - Mus musculusNOV21tSNP13375139543544Netrin receptor Unc5h1 - Mus musculusNOV21uSNP13375496545546Netrin receptor Unc5h1 - Mus musculusNOV22aCG53944-02547548human secreted polypeptide - Homo sapiensNOV22bCG53944-01549550human secreted polypeptide - Homo sapiensNOV23aCG54308-04551552Serpin B12 - Homo sapiensNOV23bCG54308-01553554Serpin B12 - Homo sapiensNOV23cCG54308-02555556Serpin B12 - Homo sapiensNOV23dCG54308-03557558Serpin B12 - Homo sapiensNOV23eCG54308-05559560Serpin B12 - Homo sapiensNOV24aCG54764-02561562Calgizzarin (Endothelial monocyte-activatingpolypeptide) (EMAP) - Mus musculusNOV24bCG54764-01563564Calgizzarin (Endothelial monocyte-activatingpolypeptide) (EMAP) - Mus musculusNOV25aCG55033-04565566Human toll like receptor like molecule 5 (TLR-L5)NOV25bCG55033-05567568Human toll like receptor like molecule 5 (TLR-L5)NOV25cCG55033-01569570Human toll like receptor like molecule 5 (TLR-L5)NOV25d237376826571572Human toll like receptor like molecule 5 (TLR-L5)NOV25e310658403573574Human toll like receptor like molecule 5 (TLR-L5)NOV25f317980876575576Human toll like receptor like molecule 5 (TLR-L5)NOV25g318018151577578Human toll like receptor like molecule 5 (TLR-L5)NOV25h318176316579580Human toll like receptor like molecule 5 (TLR-L5)NOV25iCG55033-02581582Human toll like receptor like molecule 5 (TLR-L5)NOV25jCG55033-03583584Human toll like receptor like molecule 5 (TLR-L5)NOV25kCG55033-06585586Human toll like receptor like molecule 5 (TLR-L5)NOV25lCG55033-07587588Human toll like receptor like molecule 5 (TLR-L5)NOV25mCG55033-08589590Human toll like receptor like molecule 5 (TLR-L5)NOV25nCG55033-09591592Human toll like receptor like molecule 5 (TLR-L5)NOV25oCG55033-10593594Human toll like receptor like molecule 5 (TLR-L5)NOV25pCG55033-11595596Human toll like receptor like molecule 5 (TLR-L5)NOV25qCG55033-12597598Human toll like receptor like molecule 5 (TLR-L5)NOV25rCG55033-13599600Human toll like receptor like molecule 5 (TLR-L5)NOV25sCG55033-14601602Human toll like receptor like molecule 5 (TLR-L5)NOV26aCG55117-04603604Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV26bCG55117-01605606Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV26cCG55117-02607608Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV26dCG55117-03609610Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV26eCG55117-05611612Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV26fSNP13382440613614Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV26gSNP13382439615616Prominin-like protein 1 precursor(Antigen AC133) (CD133 antigen) - Homo sapiensNOV27aCG55193-04617618PLVAP protein - Homo sapiens (Human), 455 aaNOV27b214575880619620PLVAP protein - Homo sapiens (Human), 455 aaNOV27c214458684621622PLVAP protein - Homo sapiens (Human), 455 aaNOV27d214458688623624PLVAP protein - Homo sapiens (Human), 455 aaNOV27eCG55193-01625626PLVAP protein - Homo sapiens (Human), 455 aaNOV27fCG55193-02627628PLVAP protein - Homo sapiens (Human), 455 aaNOV27gCG55193-03629630PLVAP protein - Homo sapiens (Human), 455 aaNOV27hCG55193-05631632PLVAP protein - Homo sapiens (Human), 455 aaNOV27iCG55193-06633634PLVAP protein - Homo sapiens (Human), 455 aaNOV27jCG55193-07635636PLVAP protein - Homo sapiens (Human), 455 aaNOV28aCG55256-07637638Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28bCG55256-10639640Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28c272511714641642Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28dCG55256-01643644Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28eCG55256-02645646Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28fCG55256-03647648Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28gCG55256-04649650Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28hCG55256-05621652Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28iCG55256-06653654Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28jCG55256-08655656Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28kCG55256-09657658Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28lCG55256-11659660Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28mSNP13382497661662Apolipoprotein E receptor 2 precursor - Homo sapiensNOV28nSNP13382498663664Apolipoprotein E receptor 2 precursor - Homo sapiensNOV29aCG55776-01665666Human osteoclast protein (OCP)NOV29b248210167667668Human osteoclast protein (OCP)NOV29c247679561669670Human osteoclast protein (OCP)NOV29d248057904671672Human osteoclast protein (OCP)NOV29e248057927673674Human osteoclast protein (OCP)NOV29f249239821675676Human osteoclast protein (OCP)NOV29g248057920677678Human osteoclast protein (OCP)NOV29h247679541679680Human osteoclast protein (OCP)NOV29i249116954681682Human osteoclast protein (OCP)NOV29j248210155683684Human osteoclast protein (OCP)NOV29k248213764685686Human osteoclast protein (OCP)NOV29l248213768687688Human osteoclast protein (OCP)NOV29m248213772689690Human osteoclast protein (OCP)NOV29n248586774691692Human osteoclast protein (OCP)NOV29o248586793693694Human osteoclast protein (OCP)NOV29p248586820695696Human osteoclast protein (OCP)NOV29q248586824697698Human osteoclast protein (OCP)NOV29r247679817699700Human osteoclast protein (OCP)NOV29s248210264701702Human osteoclast protein (OCP)NOV29t248210551703704Human osteoclast protein (OCP)NOV29u248210824705706Human osteoclast protein (OCP)NOV29v248679541707708Human osteoclast protein (OCP)NOV29w247679454709710Human osteoclast protein (OCP)NOV29x314361407711712Human osteoclast protein (OCP)NOV29y317803448713714Human osteoclast protein (OCP)NOV29zCG55776-02715716Human osteoclast protein (OCP)NOV29aaCG55776-03717718Human osteoclast protein (OCP)NOV29abCG55776-04719720Human osteoclast protein (OCP)NOV29acCG55776-05721722Human osteoclast protein (OCP)NOV29adCG55776-06723724Human osteoclast protein (OCP)NOV29aeCG55776-07725726Human osteoclast protein (OCP)NOV29afCG55776-08727728Human osteoclast protein (OCP)NOV29agCG55776-09729730Human osteoclast protein (OCP)NOV29ahSNP13376522731732Human osteoclast protein (OCP)NOV29aiSNP13377624733734Human osteoclast protein (OCP)NOV29ajSNP13377625735736Human osteoclast protein (OCP)NOV29akSNP13376523737738Human osteoclast protein (OCP)NOV29alSNP13376524739740Human osteoclast protein (OCP)NOV29amSNP13376525741742Human osteoclast protein (OCP)NOV29anSNP13376526743744Human osteoclast protein (OCP)NOV29aoSNP13377626745746Human osteoclast protein (OCP)NOV29apSNP13377641747748Human osteoclast protein (OCP)NOV29aqSNP13377640749750Human osteoclast protein (OCP)NOV30aCG55784-03751752NPEH2 - Homo sapiensNOV30bCG55784-01753754NPEH2 - Homo sapiensNOV30c312000579755756NPEH2 - Homo sapiensNOV30d311333341757758NPEH2 - Homo sapiensNOV30e311333338759760NPEH2 - Homo sapiensNOV30f311346885761762NPEH2 - Homo sapiensNOV30g311333299763764NPEH2 - Homo sapiensNOV30hCG55784-02765766NPEH2 - Homo sapiensNOV30iSNP13376439767768NPEH2 - Homo sapiensNOV31aCG55790-02769770ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31b258668431771772ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31c309303509773774ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31d315925314775776ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31e315970230777778ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31fCG55790-01779780ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31gCG55790-03781782ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31hCG55790-04783784ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31iCG55790-05785786ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31jCG55790-06787788ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31kCQ55790-07789790ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV31lSNP13374852791792ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-likeprotein G150) (B7-related protein-1) (B7RP-1) - Homo sapiensNOV32aCG55906-04793794Human breast tumour-associated protein 37NOV32bCG55906-05795796Human breast tumour-associated protein 37NOV32cCG55906-01797798Human breast tumour-associated protein 37NOV32d277901794799800Human breast tumour-associated protein 37NOV32eCG55906-02801802Human breast tumour-associated protein 37NOV32f230272941803804Human breast tumour-associated protein 37NOV32gCG55906-03805806Human breast tumour-associated protein 37NOV32hSNP13382505807808Human breast tumour-associated protein 37NOV32iSNP13382504809810Human breast tumour-associated protein 37NOV32jSNP13376440811812Human breast tumour-associated protein 37NOV33aCG55908-01813814integrin alpha-7 chain precursor - humanNOV33b253116407815816integrin alpha-7 chain precursor - humanNOV33c253116412817818integrin alpha-7 chain precursor - humanNOV34aCG56077-06819820LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34bCG56077-01821822LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34cCG56077-02823824LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34dCG56077-03825826LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34eCG56077-04827828LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34fCG56077-05829830LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34gSNP13374715831832LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV34hSNP13374714833834LGI4 (Leucine-rich glioma inactivated protein 4)(LGI1-like protein 3) - Homo sapiensNOV35aCG56110-03835836B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35bCG56110-07837838B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35c274082305839840B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35dCG56110-01841842B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35eCG56110-02843844B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35fCG56110-04845846B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35gCG56110-05847848B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV35hCG56110-06849850B7-H1 (PD-1-ligand precursor) - Homo sapiensNOV36aCG56383-02851852Prickle-like 1 (Drosophila) - Mus musculus (Mouse), 879 aaNOV36bCG56383-01853854Prickle-like 1 (Drosophila) - Mus musculus (Mouse), 879 aaNOV36cSNP13382513855856Prickle-like 1 (Drosophila) - Mus musculus (Mouse), 879 aaNOV37aCG56449-04857858MEGF6 - Homo sapiens (Human), 1246 aaNOV37bCG56449-09859860MEGF6 - Homo sapiens (Human), 1246 aaNOV37c191887507861862MEGF6 - Homo sapiens (Human), 1246 aaNOV37d316351371863864MEGF6 - Homo sapiens (Human), 1246 aaNOV37e316935396865866MEGF6 - Homo sapiens (Human), 1246 aaNOV37f317004318867868MEGF6 - Homo sapiens (Human), 1246 aaNOV37gCG56449-01869870MEGF6 - Homo sapiens (Human), 1246 aaNOV37hCG56449-02871872MEGF6 - Homo sapiens (Human), 1246 aaNOV37iCG56449-03873874MEGF6 - Homo sapiens (Human), 1246 aaNOV37jCG56449-05875876MEGF6 - Homo sapiens (Human), 1246 aaNOV37kCG56449-06877878MEGF6 - Homo sapiens (Human), 1246 aaNOV37lCG56449-07879880MEGF6 - Homo sapiens (Human), 1246 aaNOV37mCG56449-08881882MEGF6 - Homo sapiens (Human), 1246 aaNOV37nCG56449-10883884MEGF6 - Homo sapiens (Human), 1246 aaNOV37oCG56449-11885886MEGF6 - Homo sapiens (Human), 1246 aaNOV37pSNP13382514887888MEGF6 - Homo sapiens (Human), 1246 aaNOV38aCG56594-01889890Claudin-19 - Homo sapiensNOV38b277685552891892Claudin-19 - Homo sapiensNOV38c277685649893894Claudin-19 - Homo sapiensNOV38d277685616895896Claudin-19 - Homo sapiensNOV39aCG56653-08897898ficolin-1 precursorNOV39b214374274899900ficolin-1 precursorNOV39c214374256901902ficolin-1 precursorNOV39d214374252903904ficolin-1 precursorNOV39e214458492905906ficolin-1 precursorNOV39f214458488907908ficolin-1 precursorNOV39g214374236909910ficolin-1 precursorNOV39hCG56653-01911912ficolin-1 precursorNOV39iCG56653-02913914ficolin-1 precursorNOV39jCG56653-03915916ficolin-1 precursorNOV39kCG56653-04917918ficolin-1 precursorNOV39lCG56653-05919920ficolin-1 precursorNOV39mCG56653-06921922ficolin-1 precursorNOV39nCG56653-07923924ficolin-1 precursorNOV39oCG56653-09925926ficolin-1 precursorNOV39pCG56653-10927928ficolin-1 precursorNOV39qCG56653-11929930ficolin-1 precursorNOV39rCG56653-12931932ficolin-1 precursorNOV40aCG56806-01933934Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40b248061366935936Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40c246837961937938Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40d246837965939940Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40e246837975941942Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40f248061376943944Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40gSNP13381685945946Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV40hSNP13381686947948Heparan sulfate 6-O-sulfotransferase 3 - Homo sapiensNOV41aCG56878-01949950Protein OS-9 precursor - Homo sapiensNOV41b175070399951952Protein OS-9 precursor - Homo sapiensNOV41c175070432953954Protein OS-9 precursor - Homo sapiensNOV41d175070419955956Protein OS-9 precursor - Homo sapiensNOV41e175070438957958Protein OS-9 precursor - Homo sapiensNOV41f175070408959960Protein OS-9 precursor - Homo sapiensNOV41gCG56878-02961962Protein OS-9 precursor - Homo sapiensNOV41hCG56878-03963964Protein OS-9 precursor - Homo sapiensNOV41iCG56878-04965966Protein OS-9 precursor - Homo sapiensNOV41jSNP13382511967968Protein OS-9 precursor - Homo sapiensNOV41kSNP13382506969970Protein OS-9 precursor - Homo sapiensNOV42aCG56904-01971972secreted protein LP255(a)NOV42b272355087973974secreted protein LP255(a)NOV42c246862506975976secreted protein LP255(a)NOV42dCG56904-04977978secreted protein LP255(a)NOV42e268824694979980secreted protein LP255(a)NOV42f283146542981982secreted protein LP255(a)NOV42g308521214983984secreted protein LP255(a)NOV42hCG56904-02985986secreted protein LP255(a)NOV42iCG56904-03987988secreted protein LP255(a)NOV42jCG56904-05989990secreted protein LP255(a)NOV42kCG56904-06991992secreted protein LP255(a)NOV42lCG56904-07993994secreted protein LP255(a)NOV42mCG56904-08995996secreted protein LP255(a)NOV43aCG56914-01997998Hemicentin - Homo sapiensNOV43b2628023679991000Hemicentin - Homo sapiensNOV43cCG56914-0210011002Hemicentin - Homo sapiensNOV43dCG56914-0310031004Hemicentin - Homo sapiensNOV44aCG56959-0210051006Synaptotagmin 10 - Rattus norvegicusNOV44bCG56959-0110071008Synaptotagmin 10 - Rattus norvegicusNOV45aCG57111-0110091010Protocadherin 13 - Homo sapiens (Human), 947 aaNOV45b27772632810111012Protocadherin 13 - Homo sapiens (Human), 947 aaNOV45cCG57111-0210131014Protocadherin 13 - Homo sapiens (Human), 947 aaNOV45dCG57111-0310151016Protocadherin 13 - Homo sapiens (Human), 947 aaNOV45eCG57111-0410171018Protocadherin 13 - Homo sapiens (Human), 947 aaNOV46aCG57409-0510191020Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46b27773144610211022Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46cCG57409-0710231024Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46d31210287410251026Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46e31210289910271028Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46fCG57409-0110291030Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46gCG57409-0210311032Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46hCG57409-0310331034Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46iCG57409-0410351036Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46jCG57409-0610371038Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV46kCG57409-0810391040Glycosyl-phosphatidyl-inositol-MAM - Homo sapiensNOV47aCG57448-0110411042Human cadherin-1 (CDHN-1) proteinNOV47b24784670510431044Human cadherin-1 (CDHN-1) proteinNOV47c24784670810451046Human cadherin-1 (CDHN-1) proteinNOV47d23758029510471048Human cadherin-1 (CDHN-1) proteinNOV47e23757951210491050Human cadherin-1 (CDHN-1) proteinNOV47fCG57448-0210511052Human cadherin-1 (CDHN-1) proteinNOV48aCG57574-0310531054Tectorin beta - Homo sapiensNOV48bCG57574-0110551056Tectorin beta - Homo sapiensNOV48cCG57574-0210571058Tectorin beta - Homo sapiensNOV48dCG57574-0410591060Tectorin beta - Homo sapiensNOV48eCG57574-0510611062Tectorin beta - Homo sapiensNOV49aCG57689-0110631064Inferred: hemicentin - Mus musculusNOV50aCG58567-0110651066Human cadherin (CAD) proteinNOV50b22008764610671068Human cadherin (CAD) proteinNOV50c19487796010691070Human cadherin (CAD) proteinNOV50d19258929710711072Human cadherin (CAD) proteinNOV50eCG58567-0210731074Human cadherin (CAD) proteinNOV50fCG58567-0310751076Human cadherin (CAD) proteinNOV50gCG58567-0410771078Human cadherin (CAD) proteinNOV50hCG58567-0510791080Human cadherin (CAD) proteinNOV50iCG58567-0610811082Human cadherin (CAD) proteinNOV51aCG59534-0310831084Membrane glycoprotein LIG-1 - Homo sapiensNOV51bCG59534-0110851086Membrane glycoprotein LIG-1 - Homo sapiensNOV51cCG59534-0210871088Membrane glycoprotein LIG-1 - Homo sapiensNOV51dSNP1337725510891090Membrane glycoprotein LIG-1 - Homo sapiensNOV52aCG59584-0310911092Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52bCG59584-0210931094Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52c24821040510951096Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52d24821043610971098Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52e24935773710991100Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52f24821043011011102Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52g24935775511031104Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV52hCG59584-0111051106Ovostatin precursor (Ovomacroglobulin) - Gallus gallusNOV53aCG56008-0111071108NOV54aCG59905-0111091110CUB and sushi multiple domains 1 protein - Homo sapiensNOV54bCG59905-0311111112CUB and sushi multiple domains 1 protein - Homo sapiensNOV54c27563110211131114CUB and sushi multiple domains 1 protein - Homo sapiensNOV54dCG59905-0211151116CUB and sushi multiple domains 1 protein - Homo sapiensNOV54eSNP1338252711171118CUB and sushi multiple domains 1 protein - Homo sapiensNOV54fSNP1338252011191120CUB and sushi multiple domains 1 protein - Homo sapiensNOV55aCG59932-0111211122secreted protein sequenceNOV56aCG92715-0111231124secreted protein sequenceNOV56b24857623311251126secreted protein sequenceNOV56cCG92715-0211271128secreted protein sequenceNOV56dCG92715-0311291130secreted protein sequenceNOV57aCG92813-0111311132Cadherin-related tumor suppressor precursor (Fat protein) -Drosophila melanogasterNOV57b26050097111331134Cadherin-related tumor suppressor precursor (Fat protein) -Drosophila melanogasterNOV57c26050096111351136Cadherin-related tumor suppressor precursor (Fat protein) -Drosophila melanogasterNOV57d25807633111371138Cadherin-related tumor suppressor precursor (Fat protein) -Drosophila melanogasterNOV57e25807634911391140Cadherin-related tumor suppressor precursor (Fat protein) -Drosophila melanogasterNOV57fCG92813-0211411142Cadherin-related tumor suppressor precursor (Fat protein) -Drosophila melanogasterNOV58aCG93387-0511431144insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV58bCG93387-0111451146insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV58cCG93387-0211471148insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV58dCG93387-0311491150insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV58eCG93387-0411511152insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV58fSNP1338251911531154insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV59aCG93871-0111551156insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV59bCG93871-0511571158insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV59c19848843211591160insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV59d19848842411611162insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV59e19848842811631164insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV59f19848844011651166insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV59gCG93871-0211671168insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV59hCG93871-0311691170insulin-responsive sequence DNA winding protein-1 - Homo sapiensNOV59iCG93871-0411711172insulin-responsive sequence DNA binding protein-1 - Homo sapiensNOV60aCG94946-0111731174AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60b27563159011751176AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60c27563156411771178AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60dCG94946-0211791180AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60eCG94946-0311811182AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60fCG94946-0411831184AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60gCG94946-0511851186AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60hCG94946-0611871188AGRIN precursor - Homo sapiens (Human), 2026 aaNOV60iCG94946-0711891190AGRIN precursor - Homo sapiens (Human), 2026 aaNOV61aCG96384-0111911192colon cancer antigen proteinNOV61b27758074511931194colon cancer antigen proteinNOV61cCG96384-0211951196colon cancer antigen proteinNOV61dCG96384-0311971198colon cancer antigen proteinNOV61eCG96384-0411991200colon cancer antigen proteinNOV62aCG98011-0112011202Neuronal transmembrane protein Slitrk4 - Mus musculusNOV62b19258695612031204Neuronal transmembrane protein Slitrk4 - Mus musculusNOV62c19199900712051206Neuronal transmembrane protein Slitrk4 - Mus musculusNOV62dCG98011-0212071208Neuronal transmembrane protein Slitrk4 - Mus musculusNOV62eCG98011-0312091210Neuronal transmembrane protein Slitrk4 - Mus musculusNOV62fCG98011-0412111212Neuronal transmembrane protein Slitrk4 - Mus musculus


Table A indicates the homology of NOVX polypeptides to known protein families. Thus, the nucleic acids and polypeptides, antibodies and related compounds according to the invention corresponding to a NOVX as identified in column 1 of Table A will be useful in therapeutic and diagnostic applications implicated in, for example, pathologies and disorders associated with the known protein families identified in column 5 of Table A.


Pathologies, diseases, disorders and condition and the like that are associated with NOVX sequences include, but are not limited to: e.g., cardiomyopathy, atherosclerosis, hypertension, congenital heart defects, aortic stenosis, atrial septal defect (ASD), vascular calcification, fibrosis, atrioventricular (A-V) canal defect, ductus arteriosus, pulmonary stenosis, subaortic stenosis, ventricular septal defect (VSD), valve diseases, tuberous sclerosis, scleroderma, obesity, metabolic disturbances associated with obesity, transplantation, osteoarthritis, rheumatoid arthritis, osteochondrodysplasia, adrenoleukodystrophy, congenital adrenal hyperplasia, prostate cancer, diabetes, metabolic disorders, neoplasm; adenocarcinoma, lymphoma, uterus cancer, fertility, glomerulonephritis, hemophilia, hypercoagulation, idiopathic thrombocytopenic purpura, immunodeficiencies, psoriasis, skin disorders, graft versus host disease, AIDS, bronchial asthma, lupus, Crohn's disease; inflammatory bowel disease, ulcerative colitis, multiple sclerosis, treatment of Albright Hereditary Ostoeodystrophy, infectious disease, anorexia, cancer-associated cachexia, cancer, neurodegenerative disorders, Alzheimer's Disease, Parkinson's Disorder, immune disorders, hematopoietic disorders, and the various dyslipidemias, schizophrenia, depression, asthma, emphysema, allergies, the metabolic syndrome X and wasting disorders associated with chronic diseases and various cancers, as well as conditions such as transplantation, neuroprotection, fertility, or regeneration (in vitro and in vivo).


NOVX nucleic acids and their encoded polypeptides are useful in a variety of applications and contexts. The various NOVX nucleic acids and polypeptides according to the invention are useful as novel members of the protein families according to the presence of domains and sequence relatedness to previously described proteins. Additionally, NOVX nucleic acids and polypeptides can also be used to identify proteins that are members of the family to which the NOVX polypeptides belong.


Consistent with other known members of the family of proteins, identified in column 5 of Table A, the NOVX polypeptides of the present invention show homology to, and contain domains that are characteristic of, other members of such protein families. Details of the sequence relatedness and domain analysis for each NOVX are presented in Example A.


The NOVX nucleic acids and polypeptides can also be used to screen for molecules, which inhibit or enhance NOVX activity or function. Specifically, the nucleic acids and polypeptides according to the invention may be used as targets for the identification of small molecules that modulate or inhibit diseases associated with the protein families listed in Table A.


The NOVX nucleic acids and polypeptides are also useful for detecting specific cell types. Details of the expression analysis for each NOVX are presented in Example C. Accordingly, the NOVX nucleic acids, polypeptides, antibodies and related compounds according to the invention will have diagnostic and therapeutic applications in the detection of a variety of diseases with differential expression in normal vs. diseased tissues, e.g. detection of a variety of cancers.


Additional utilities for NOVX nucleic acids and polypeptides according to the invention are disclosed herein.


NOVX Clones


NOVX nucleic acids and their encoded polypeptides are useful in a variety of applications and contexts. The various NOVX nucleic acids and polypeptides according to the invention are useful as novel members of the protein families according to the presence of domains and sequence relatedness to previously described proteins. Additionally, NOVX nucleic acids and polypeptides can also be used to identify proteins that are members of the family to which the NOVX polypeptides belong.


The NOVX genes and their corresponding encoded proteins are useful for preventing, treating or ameliorating medical conditions, e.g., by protein or gene therapy. Pathological conditions can be diagnosed by determining the amount of the new protein in a sample or by determining the presence of mutations in the new genes. Specific uses are described for each of the NOVX genes, based on the tissues in which they are most highly expressed. Uses include developing products for the diagnosis or treatment of a variety of diseases and disorders.


The NOVX nucleic acids and proteins of the invention are useful in potential diagnostic and therapeutic applications and as a research tool. These include serving as a specific or selective nucleic acid or protein diagnostic and/or prognostic marker, wherein the presence or amount of the nucleic acid or the protein are to be assessed, as well as potential therapeutic applications such as the following: (i) a protein therapeutic, (ii) a small molecule drug target, (iii) an antibody target (therapeutic, diagnostic, drug targeting/cytotoxic antibody), (iv) a nucleic acid useful in gene therapy (gene delivery/gene ablation), and (v) a composition promoting tissue regeneration in vitro and in vivo (vi) a biological defense weapon.


In one specific embodiment, the invention includes an isolated polypeptide comprising an amino acid sequence selected from the group consisting of: (a) a mature form of the amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606; (b) a variant of a mature form of the amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606, wherein any amino acid in the mature form is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence of the mature form are so changed; (c) an amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606; (d) a variant of the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 wherein any amino acid specified in the chosen sequence is changed to a different amino acid,.provided that no more than 15% of the amino acid residues in the sequence are so changed; and (e) a fragment of any of (a) through (d).


In another specific embodiment, the invention includes an isolated nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of: (a) a mature form of the amino acid sequence given SEQ ID NO: 2n, wherein n is an integer between 1 and 606; (b) a variant of a mature form of the amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606 wherein any amino acid in the mature form of the chosen sequence is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence of the mature form are so changed; (c) the amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606; (d) a variant of the amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606, in which any amino acid specified in the chosen sequence is changed to a different amino acid, provided that no more than 15% of the amino acid residues in the sequence are so changed; (e) a nucleic acid fragment encoding at least a portion of a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 2n, wherein n is an integer between 1 and 606 or any variant of said polypeptide wherein any amino acid of the chosen sequence is changed to a different amino acid, provided that no more than 10% of the amino acid residues in the sequence are so changed; and (f) the complement of any of said nucleic acid molecules.


In yet another specific embodiment, the invention includes an isolated nucleic acid molecule, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence selected from the group consisting of SEQ ID NO: 2n−1, wherein n is an integer between 1 and 606; (b) a nucleotide sequence wherein one or more nucleotides in the nucleotide sequence selected from the group consisting of SEQ ID NO: 2n−1, wherein n is an integer between 1 and 606 is changed from that selected from the group consisting of the chosen sequence to a different nucleotide provided that no more than 15% of the nucleotides are so changed; (c) a nucleic acid fragment of the sequence selected from the group consisting of SEQ ID NO: 2n−1, wherein n is an integer between 1 and 606; and (d) a nucleic acid fragment wherein one or more nucleotides in the nucleotide sequence selected from the group consisting of SEQ ID NO: 2n−1, wherein n is an integer between 1 and 606 is changed from that selected from the group consisting of the chosen sequence to a different nucleotide provided that no more than 15% of the nucleotides are so changed.


NOVX Nucleic Acids and Polypeptides


One aspect of the invention pertains to isolated nucleic acid molecules that encode NOVX polypeptides or biologically active portions thereof. Also included in the invention are nucleic acid fragments sufficient for use as hybridization probes to identify NOVX-encoding nucleic acids (e.g., NOVX mRNAs) and fragments for use as PCk primers for the amplification and/or mutation of NOVX nucleic acid molecules. As used herein, the term “nucleic acid molecule” is intended to include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules (e.g., mRNA), analogs of the DNA or RNA generated using nucleotide analogs, and derivatives, fragments and homologs thereof. The nucleic acid molecule may be single-stranded or double-stranded, but preferably is comprised double-stranded DNA.


A NOVX nucleic acid can encode a mature NOVX polypeptide. As used herein, a “mature” form of a polypeptide or protein disclosed in the present invention isthe product of a naturally occurring polypeptide or precursor form or proprotein. The naturally occurring polypeptide, precursor or proprotein includes, by way of nonlimiting example, the full-length gene product encoded by the corresponding gene. Alternatively, it may be defined as the polypeptide, precursor or proprotein encoded by an ORF described herein. The product “mature” form arises, by way of nonlimiting example, as a result of one or more naturally occurring processing steps that may take place within the cell (e.g., host cell) in which the gene product arises. Examples of such processing steps leading to a “mature” form of a polypeptide or protein include the cleavage of the N-terminal methionine residue encoded by the initiation codon of an ORF, or the proteolytic cleavage of a signal peptide or leader sequence. Thus a mature form arising from a precursor polypeptide or protein that has residues 1 to N, where residue 1 is the N-terminal methionine, would have residues 2 through N remaining after removal of the N-terminal methionine. Alternatively, a mature form arising from a precursor polypeptide or protein having residues 1 to N, in which an N-terminal signal sequence from residue 1 to residue M is cleaved, would have the residues from residue M+1 to residue N remaining. Further as used herein, a “mature” form of a polypeptide or protein may arise from a step of post-translational modification other than a proteolytic cleavage event. Such additional processes include, by way of non-limiting example, glycosylation, myristylation or phosphorylation. In general, a mature polypeptide or protein may result from the operation of only one of these processes, or a combination of any of them.


The term “probe”, as utilized herein, refers to nucleic acid sequences of variable length, preferably between at least about 10 nucleotides (nt), about 100 nt, or as many as approximately, e.g., 6,000 nt, depending upon the specific use. Probes are used in the detection of identical, similar, or complementary nucleic acid sequences. Longer length probes are generally obtained from a natural or recombinant source, are highly specific, and much slower to hybridize than shorter-length oligomer probes. Probes may be single-stranded or double-stranded and designed to have specificity in PCR, membrane-based hybridization technologies, or ELISA-like technologies.


The term “isolated” nucleic acid molecule, as used herein, is a nucleic acid that is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an “isolated” nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5′- and 3′-termini of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated NOVX nucleic acid molecules can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell/tissue from which the nuicleic acid is derived (e.g., brain, heart, liver, spleen, etc.). Moreover, an “isolated” nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material, or culture medium, or of chemical precursors or other chemicals.


A nucleic acid molecule of the invention, e.g., a nucleic acid molecule having the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or a complement of this nucleotide sequence, can be isolated using standard molecular biology techniques and the sequence information provided herein. Using all or a portion of the nucleic acid sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, as a hybridization probe, NOVX molecules can be isolated using standard hybridization and cloning techniques (e.g., as described in Sambrook, et al., (eds.), MOLECULAR CLONING: A LABORATORY MANUAL 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989; and Ausubel, et al., (eds.), CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, New York, N.Y., 1993.) A nucleic acid of the invention can be amplified using cDNA, mRNA or alternatively, genomicDNA, as a template with appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to NOVX nucleotide sequences can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.


As used herein, the term “oligonucleotide” refers to a series of linked nucleotide residues. A short oligonucleotide sequence may be based on, or designed from, a genomic or cDNA sequence and is used to amplify, confirm, or reveal the presence of an identical, similar or complementary DNA or RNA in a particular cell or tissue. Oligonucleotides comprise a nucleic acid sequence having about 10 nt, 50 nt, or 100 nt in length, preferably about 15 nt to 30 nt in length. In one embodiment of the invention, an oligonucleotide comprising a nucleic acid molecule less than 100 nt in length would further comprise at least 6 contiguous nucleotides of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or a complement thereof. Oligonucleotides may be chemically synthesized and may also be used as probes.


In another embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule that is a complement of the nucleotide sequence shown in SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or a portion of this nucleotide sequence (e.g., a fragment that can be used as a probe or primer or a fragment encoding a biologically-active portion of a NOVX polypeptide). A nucleic acid molecule that is complementary to the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, is one that is sufficiently complementary to the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, that it can hydrogen bond with few or no mismatches to the nucleotide sequence shown in SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, thereby forming a stable duplex.


As used herein, the term “complementary” refers to Watson-Crick or Hoogsteen base pairing between nucleotides units of a nucleic acid molecule, and the term “binding” means the physical or chemical interaction between two polypeptides or compounds or associated polypeptides or compounds or combinations thereof. Binding includes ionic, non-ionic, van der Waals, hydrophobic interactions, and the like. A physical interaction can be either direct or indirect. Indirect interactions may be through or due to the effects of another polypeptide or compound. Direct binding refers to interactions that do not take place through, or due to, the effect of another polypeptide or compound, but instead are without other substantial chemical intermediates.


A “fragment” provided herein is defined as a sequence of at least 6 (contiguous) nucleic acids or at least 4 (contiguous) amino acids, a length sufficient to allow for specific hybridization in the case of nucleic acids or for specific recognition of an epitope in the case of amino acids, and is at most some portion less than a full length sequence. Fragments may be derived from any contiguous portion of a nucleic acid or amino acid sequence of choice.


A full-length NOVX clone is identified as containing an ATG translation start codon and an in-frame stop codon. Any disclosed NOVX nucleotide sequence lacking an ATG start codon therefore encodes a truncated C-terminal fragment of the respective NOVX polypeptide, and requires that the corresponding full-length cDNA extend in the 5′ direction of the disclosed sequence. Any disclosed NOVX nucleotide sequence lacking an in-frame stop codon similarly encodes a truncated N-terminal fragment of the respective NOVX polypeptide, and requires that the corresponding full-length cDNA extend in the 3′ direction of the disclosed sequence.


A “derivative” is a nucleic acid sequence or amino acid sequence formed from the native compounds either directly, by modification or partial substitution. An “analog” is a nucleic acid sequence or amino acid sequence that has a structure similar to, but not identical to, the native compound, e.g. they differs from it in respect to certain components or side chains. Analogs may be synthetic or derived from a different evolutionary origin and may have a similar or opposite metabolic activity compared to wild type. A “homolog” is a nucleic acid sequence or amino acid sequence of a particular gene that is derived from different species.


Derivatives and analogs may be full length or other than full length. Derivatives or analogs of the nucleic acids or proteins of the invention include, but are not limited to, molecules comprising regions that are substantially homologous to the nucleic acids or proteins of the invention, in various embodiments, by at least about 70%, 80%, or 95% identity (with a preferred identity of 80-95%) over a nucleic acid or amino acid sequence of identical size or when compared to an aligned sequence in which the alignment is done by a computer homology program known in the art, or whose encoding nucleic acid is capable of hybridizing to the complement of a sequence encoding the proteins under stringent, moderately stringent, or low stringent conditions. See e.g. Ausubel, et al., CURENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, New York, N.Y., 1993, and below.


A “homologous nucleic acid sequence” or “homologous amino acid sequence,” or variations thereof, refer to sequences characterized by a homology at the nucleotide level or amino acid level as discussed above. Homologous nucleotide sequences include those sequences coding for isoforms of NOVX polypeptides. Isoforms can be expressed in different tissues of the same organism as a result of, for example, alternative splicing of RNA. Alternatively, isoforms can be encoded by different genes. In the invention, homologous nucleotide sequences include nucleotide sequences encoding for a NOVX polypeptide of species other than humans, including, but not limited to: vertebrates, and thus can include, e.g., frog, mouse, rat, rabbit, dog, cat cow, horse, and other organisms. Homologous nucleotide sequences also include, but are not limited to, naturally occurring allelic variations and mutations of the nucleotide sequences set forth herein. A homologous nucleotide sequence does not, however, include the exact nucleotide sequence encoding human NOVX protein. Homologous nucleic acid sequences include those nucleic acid sequences that encode conservative amino acid substitutions (see below) in SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, as well as a polypeptide possessing NOVX biological activity. Various biological activities of the NOVX proteins are described below.


A NOVX polypeptide is encoded by the open reading frame (“ORF”) of a NOVX nucleic acid. An ORF corresponds to a nucleotide sequence that could potentially be translated into a polypeptide. A stretch of nucleic acids comprising an ORF is uninterrupted by a stop codon. An ORF that represents the coding sequence for a full protein begins with an ATG “start” codon and terminates with one of the three “stop” codons, namely, TAA, TAG, or TGA. For the purposes of this invention, an ORF may be any part of a coding sequence, with or without a start codon, a stop codon, or both. For an ORF to be considered as a good candidate for coding for a bonafide cellular protein, a minimum size requirement is often set, e.g., a stretch of DNA that would encode a protein of 50 amino acids or more.


The nucleotide sequences determined from the cloning of the human NOVX genes allows for the generation of probes and primers designed for use in identifying and/or cloning NOVX homologues in other cell types, e.g. from other tissues, as well as NOVX homologues from other vertebrates. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 25, 50, 100, 150, 200, 250, 300, 350 or 400 consecutive sense strand nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606; or an anti-sense strand nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606; or of a naturally occurring mutant of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606.


Probes based on the human NOVX nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In various embodiments, the probe has a detectable label attached, e.g. the label can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor. Such probes can be used as a part of a diagnostic test kit for identifying cells or tissues which mis-express a NOVX protein, such as by measuring a level of a NOVX-encoding nucleic acid in a sample of cells from a subject e.g., detecting NOVX mRNA levels or determining whether a genomic NOVX gene has been mutated or deleted. “A polypeptide having a biologically-active portion of a NOVX polypeptide” refers to polypeptides exhibiting activity similar, but not necessarily identical to, an activity of a polypeptide of the invention, including mature forms, as measured in a particular biological assay, with or without dose dependency. A nucleic acid fragment encoding a “biologically-active portion of NOVX” can be prepared by isolating a portion of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, that encodes a polypeptide having a NOVX biological activity (the biological activities of the NOVX proteins are described below), expressing the encoded portion of NOVX protein (e.g., by recombinant expression in vitro) and assessing the activity of the encoded portion of NOVX.


NOVX Single Nucleotide Polymorphisms


Variant sequences are also included in this application. A variant sequence can include a single nucleotide polymorphism (SNP). A SNP can, in some instances, be referred to as a “cSNP” to denote that the nucleotide sequence containing the SNP originates as a cDNA. A SNP can arise in several ways. For example, a SNP may be due to a substitution of one nucleotide for another at the polymorphic site. Such a substitution can be either a transition or a transversion. A SNP can also arise from a deletion of a nucleotide or an insertion of a nucleotide, relative to a reference allele. In this case, the polymorphic site is a site at which one allele bears a gap with respect to a particular nucleotide in another allele. SNPs occurring within genes may result in an alteration of the amino acid encoded by the gene at the position of the SNP. Intragenic SNPs may also be silent, when a codon including a SNP encodes the same amino acid as a result of the redundancy of the genetic code. SNPs occurring outside the region of a gene, or in an intron within a gene, do not result in changes in any amino acid sequence of a protein but may result in altered regulation of the expression pattern. Examples include alteration in temporal expression, physiological response regulation, cell type expression regulation, intensity of expression, and stability of transcribed message.


SeqCalling assemblies produced by the exon linking process were selected and extended using the following criteria. Genomic clones having regions with 98% identity to all or part of the initial or extended sequence were identified by BLASTN searches using the relevant sequence to query human genomic databases. The genomic clones that resulted were selected for further analysis because this identity indicates that these clones contain the genomic locus for these SeqCalling assemblies. These sequences were analyzed for putative coding regions as well as for similarity to the known DNA and protein sequences. Programs used for these analyses include Grail, Genscan, BLAST, HMMER, FASTA, Hybrid and other relevant programs.


Some additional genomic regions may have also been identified because selected SeqCalling assemblies map to those regions. Such SeqCalling sequences may have overlapped with regions defined by homology or exon prediction. They may also be included because the location of the fragment was in the vicinity of genomic regions identified by similarity or exon prediction that had been included in the original predicted sequence. The sequence so identified was manually assembled and then may have been extended using one or more additional sequences taken from CuraGen Corporation's human SeqCalling database. SeqCalling fragments suitable for inclusion were identified by the CuraTools™ program SeqExtend or by identifying SeqCalling fragments mapping to the appropriate regions of the genomic clones analyzed.


The regions defined by the procedures described above were then manually integrated and corrected for apparent inconsistencies that may have arisen, for example, from miscalled bases in the original fragments or from discrepancies between predicted exon junctions, EST locations and regions of sequence similarity, to derive the final sequence disclosed herein. When necessary, the process to identify and analyze SeqCalling assemblies and genomic clones was reiterated to derive the full length sequence (Alderbom et al., Determination of Single Nucleotide Polymorphisms by Real-time Pyrophosphate DNA Sequencing. Genome Research. 10 (8) 1249-1265, 2000).


Variants are reported individually but any combination of all or a select subset of variants are also included as contemplated NOVX embodiments of the invention.


NOVX Nucleic Acid and Polypeptide Variants


The invention further encompasses nucleic acid molecules that differ from the nucleotide sequences of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, due to degeneracy of the genetic code and thus encode the same NOVX proteins as that encoded by the nucleotide sequences of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence of SEQ ID NO:2n, wherein n is an integer between 1 and 606.


In addition to the human NOVX nucleotide sequences of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, it will be appreciated by those skilled in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of the NOVX polypeptides may exist within a population (e.g., the human population). Such genetic polymorphism in the NOVX genes may exist among individuals within a population due to natural allelic variation. As used herein, the terms “gene” and “recombinant gene” refer to nucleic acid molecules comprising an open reading frame (ORF) encoding a NOVX protein, preferably a vertebrate NOVX protein. Such natural allelic variations can typically result in 1-5% variance in the nucleotide sequence of the NOVX genes. Any and all such nucleotide variations and resulting amino acid polymorphisms in the NOVX polypeptides, which are the result of natural allelic variation and that do not alter the functional activity of the NOVX polypeptides, are intended to be within the scope of the invention.


Moreover, nucleic acid molecules encoding NOVX proteins from other species, and thus that have a nucleotide sequence that differs from a human SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, are intended to be within the scope of the invention. Nucleic acid molecules corresponding to natural allelic variants and homologues of the NOVX cDNAs of the invention can be isolated based on their homology to the human NOVX nucleic acids disclosed herein using the human cDNAs, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions.


Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least 6 nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606. In another embodiment, the nucleic acid is at least 10, 25, 50, 100, 250, 500, 750, 1000, 1500, or 2000 or more nucleotides in length. In yet another embodiment, an isolated nucleic acid molecule of the invention hybridizes to the coding region. As used herein, the term “hybridizes under stringent conditions” is intended to describe conditions for hybridization and washing under which nucleotide sequences at least about 65% homologous to each other typically remain hybridized to each other.


Homologs (i.e., nucleic acids encoding NOVX proteins derived from species other than human) or other related sequences (e.g., paralogs) can be obtained by low, moderate or high stringency hybridization with all or a portion of the particular human sequence as a probe using methods well known in the art for nucleic acid hybridization and cloning.


As used herein, the phrase “stringent hybridization conditions” refers to conditions under which a probe, primer or oligonucleotide will hybridize to its target sequence, but to no other sequences. Stringent conditions are sequence-dependent and will be different in different circumstances. Longer sequences hybridize specifically at higher temperatures than shorter sequences. Generally, stringent conditions are selected to be about 5 ° C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength, pH and nucleic acid concentration) at which 50% of the probes complementary to the target sequence hybridize to the target sequence at equilibrium. Since the target sequences are generally present at excess, at Tm, 50% of the probes are occupied at equilibrium. Typically, stringent conditions will be those in which the salt concentration is less than about 1.0 M sodium ion, typically about 0.01 to 1.0 M sodium ion (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes, primers or oligonucleotides (e.g., 10 nt to 50 nt) and at least about 60° C. for longer probes, primers and oligonucleotides. Stringent conditions may also be achieved with the addition of destabilizing agents, such as formamide.


Stringent conditions are known to those skilled in the art and can be found in Ausubel, et al., (eds.), CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. Preferably, the conditions are such that sequences at least about 65%, 70%, 75%, 85%, 90%, 95%, 98%, or 99% homologous to each other typically remain hybridized to each other. A non-limiting example of stringent hybridization conditions are hybridization in a high salt buffer comprising 6×SSC, 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.02% BSA, and 500 mg/ml denatured salmon sperm DNA at 65° C., followed by one or more washes in 0.2×SSC, 0.01% BSA at 50° C. An isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, corresponds to a naturally-occurring nucleic acid molecule. As used herein, a “naturally-occurring” nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature (e.g., encodes a natural protein).


In a second embodiment, a nucleic acid sequence that is hybridizable to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or fragments, analogs or derivatives thereof, under conditions of moderate stringency is provided. A non-limiting example of moderate stringency hybridization conditions are hybridization in 6×SSC, 5×Reinhardt's solution, 0.5% SDS and 100 mg/ml denatured salmon sperm DNA at 55° C., followed by one or more washes in 1×SSC, 0.1% SDS at 37° C. Other conditions of moderate stringency that may be used are well-known within the art. See, e.g., Ausubel. et al. (eds.), 1993, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, NY, and Krieger, 1990; GENE TRANSFER AND EXPRESSION, A LABORATORY MANUAL, Stockton Press, N.Y.


In a third embodiment, a nucleic acid that is hybridizable to the nucleic acid molecule comprising the nucleotide sequences of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or fragments, analogs or derivatives thereof, under conditions of low stringency, is provided. A non-limiting example of low stringency hybridization conditions are hybridization in 35% formamide, 5×SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 mg/ml denatured salmon sperm DNA, 10% (wt/vol) dextran sulfate at 40° C., followed by one or more washes in 2×SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS at 50° C. Other conditions of low stringency that may be used are well known in the art (e.g., as employed for cross-species hybridizations). See, e.g., Ausubel, et al. (eds.), 1993, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, NY, and Kriegler, 1990, GENE TRANSFER AND EXPRESSION, A LABORATORY MANUAL, Stockton Press, N.Y.; Shilo and Weinberg, 1981. Proc Natl Acad Sci USA 78: 6789-6792.


Conservative Mutations


In addition to naturally-occurring allelic variants of NOVX sequences that may exist in the population, the skilled artisan will further appreciate that changes can be introduced by mutation into the nucleotide sequences of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, thereby leading to changes in the amino acid sequences of the encoded NOVX protein, without altering the functional ability of that NOVX protein. For example, nucleotide substitutions leading to amino acid substitutions at “non-essential” amino acid residues can be made in the sequence of SEQ ID NO:2n, wherein n is an integer between 1 and 606. A “non-essential” amino acid residue is a residue that can be altered from the wild-type sequences of the NOVX proteins without altering their biological activity, whereas an “essential” amino acid residue is required for such biological activity. For example, amino acid residues that are conserved among the NOVX proteins of the invention are predicted to be particularly non-amenable to alteration. Amino acids for which conservative substitutions can be made are well-known within the art.


Another aspect of the invention pertains to nucleic acid molecules encoding NOVX proteins that contain changes in amino acid residues that are not essential for activity. Such NOVX proteins differ in amino acid sequence from SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, yet retain biological activity. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 40% homologous to the amino acid sequences of SEQ ID NO:2n, wherein n is an integer between 1 and 606. Preferably, the protein encoded by the nucleic acid molecule is at least about 60% homologous to SEQ ID NO:2n, wherein n is an integer between 1 and 606; more preferably at least about 70% homologous to SEQ ID NO:2n, wherein n is an integer between 1 and 606; still more preferably at least about 80% homologous to SEQ ID NO:2n, wherein n is an integer between 1 and 606; even more preferably at least about 90% homologous to SEQ ID NO:2n, wherein n is an integer between 1 and 606; and most preferably at least about 95% homologous to SEQ ID NO:2n, wherein n is an integer between 1 and 606.


An isolated nucleic acid molecule encoding a NOVX protein homologous to the protein of SEQ ID NO:2n, wherein n is an integer between 1 and 606, can be created by introducing one or more nucleotide substitutions, additions or deletions into the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein.


Mutations can be introduced any one of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, by standard techniques, such as site-directed mutagenesis and PCR-mediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted, non-essential amino acid residues. A “conservative amino acid substitution” is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined within the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted non-essential amino acid residue in the NOVX protein is replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of a NOVX coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for NOVX biological activity to identify mutants that retain activity. Following mutagenesis of a nucleic acid of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, the encoded protein can be expressed by any recombinant technology known in the art and the activity of the protein can be determined.


The relatedness of amino acid families may also be determined based on side chain interactions. Substituted amino acids may be fully conserved “strong” residues or fully conserved “weak” residues. The “strong” group of conserved amino acid residues may be any one of the following groups: STA, NEQK, NHQK, NDEQ, QHRK, MILV, MILF, HY, FYW, wherein the single letter amino acid codes are grouped by those amino acids that may be substituted for each other. Likewise, the “weak” group of conserved residues may be any one of the following: CSA, ATV, SAG, STNK, STPA, SGND, SNDEQK, NDEQHK, NEQHRK, HFY, wherein the letters within each group represent the single letter amino acid code.


In one embodiment, a mutant NOVX protein can be assayed for (i) the ability to form protein:protein interactions with other NOVX proteins, other cell-surface proteins, or biologically-active portions thereof, (ii) complex formation between a mutant NOVX protein and a NOVX ligand; or (iii) the ability of a mutant NOVX protein to bind to an intracellular target protein or biologically-active portion thereof; (e.g. avidin proteins).


In yet another embodiment, a mutant NOVX protein can be assayed for the ability to regulate a specific biological function (e.g., regulation of insulin release).


Interfering RNA


In one aspect of the invention, NOVX gene expression can be attenuated by RNA interference. One approach well-known in the art is short interfering RNA (siRNA) mediated gene silencing where expression products of a NOVX gene are targeted by specific double stranded NOVX derived siRNA nucleotide sequences that are complementary to at least a 19-25 nt long segment of the NOVX gene transcript, including the 5′ untranslated (UT) region, the ORF, or the 3′ UT region. See, e.g., PCT applications WO00/44895, WO99/32619, WO01/75164, WO01/92513, WO 01/29058, WO01/89304, WO02/16620, and WO02/29858, each incorporated by reference herein in their entirety. Targeted genes can be a NOVX gene, or an upstream or downstream modulator of the NOVX gene. Nonlimiting examples of upstream or downstream modulators of a NOVX gene include, e.g., a transcription factor that binds the NOVX gene promoter, a kinase or phosphatase that interacts with a NOVX polypeptide, and polypeptides involved in a NOVX regulatory pathway.


According to the methods of the present invention, NOVX gene expression is silenced using short interfering RNA. A NOVX polynucleotide according to the invention includes a siRNA polynucleotide. Such a NOVX siRNA can be obtained using a NOVX polynucleotide sequence, for example, by processing the NOVX ribopolynucleotide sequence in a cell-free system, such as but not limited to a Drosophila extract, or by transcription of recombinant double stranded NOVX RNA or by chemical synthesis of nucleotide sequences homologous to a NOVX sequence. See, e.g., Tuschl, Zamore, Lehmann, Bartel and Sharp (1999), Genes & Dev. 13: 3191-3197, incorporated herein by reference in its entirety. When synthesized, a typical 0.2 micromolar-scale RNA synthesis provides about 1 milligram of siRNA, which is sufficient for 1000 transfection experiments using a 24-well tissue culture plate format.


The most efficient silencing is generally observed with siRNA duplexes composed of a 21-nt sense strand and a 21-nt antisense strand, paired in a manner to have a 2-nt 3′ overhang. The sequence of the 2-nt 3′ overhang makes an additional small contribution to the specificity of siRNA target recognition. The contribution to specificity is localized to the unpaired nucleotide adjacent to the first paired bases. In one embodiment, the nucleotides in the 3′ overhang are ribonucleotides. In an alternative embodiment, the nucleotides in the 3′ overhang are deoxyribonucleotides. Using 2′-deoxyribonucleotides in the 3′ overhangs is as efficient as using ribonucleotides, but deoxyribonucleotides are often cheaper to synthesize and are most likely more nuclease resistant.


A contemplated recombinant expression vector of the invention comprises a NOVX DNA molecule cloned into an expression vector comprising operatively-linked regulatory sequences flanking the NOVX sequence in a manner that allows for expression (by transcription of the DNA molecule) of both strands. An RNA molecule that is antisense to NOVX mRNA is transcribed by a first promoter (e.g., a promoter sequence 3′ of the cloned DNA) and an RNA molecule that is the sense strand for the NOVX mRNA is transcribed by a second promoter (e.g., a promoter sequence 5′ of the cloned DNA). The sense and antisense strands may hybridize in vivo to generate siRNA constructs for silencing of the NOVX gene. Alternatively, two constructs can be utilized to create the sense and anti-sense strands of a siRNA construct. Finally, cloned DNA can encode a construct having secondary structure, wherein a single transcript has both the sense and complementary antisense sequences from the target gene or genes. In an example of this embodiment, a hairpin RNAi product is homologous to all or a portion of the target gene. In another example, a hairpin RNAi product is a siRNA. The regulatory sequences flanking the NOVX sequence may be identical or may be different, such that their expression may be modulated independently, or in a temporal or spatial manner.


In a specific embodiment, siRNAs are transcribed intracellularly by cloning the NOVX gene templates into a vector containing, e.g., a RNA pol III transcription unit from the smaller nuclear RNA (snRNA) U6 or the human RNase P RNA H1. One example of a vector system is the GeneSuppressor™ RNA Interference kit (commercially available from Imgenex). The U6 and H1 promoters are members of the type III class of Pol III promoters. The +1 nucleotide of the U6-like promoters is always guanosine, whereas the +1 for H1 promoters is adenosine. The termination signal for these promoters is defined by five consecutive thymidines. The transcript is typically cleaved after the second uridine. Cleavage at this position generates a 3′ UU overhang in the expressed siRNA, which is similar to the 3′ overhangs of synthetic siRNAs. Any sequence less than 400 nucleotides in length can be transcribed by these promoter, therefore they are ideally suited for the expression of around 21-nucleotide siRNAs in, e.g., an approximately 50-nucleotide RNA stem-loop transcript.


A siRNA vector appears to have an advantage over synthetic siRNAs where long term knock-down of expression is desired. Cells transfected with a siRNA expression vector would experience steady, long-term mRNA inhibition. In contrast, cells transfected with exogenous synthetic siRNAs typically recover from mRNA suppression within seven days or ten rounds of cell division. The long-term gene silencing ability of siRNA expression vectors may provide for applications in gene therapy.


In general, siRNAs are chopped from longer dsRNA by an ATP-dependent ribonuclease called DICER. DICER is a member of the RNase III family of double-stranded RNA-specific endonucleases. The siRNAs assemble with cellular proteins into an endonuclease complex. In vitro studies in Drosophila suggest that the siRNAs/protein complex (siRNP) is then transferred to a second enzyme complex, called an RNA-induced silencing complex (RISC), which contains an endoribonuclease that is distinct from DICER. RISC uses the sequence encoded by the antisense siRNA strand to find and destroy mRNAs of complementary sequence. The siRNA thus acts as a guide, restricting the ribonuclease to cleave only mRNAs complementary to one of the two siRNA strands.


A NOVX mRNA region to be targeted by siRNA is generally selected from a desired NOVX sequence beginning 50 to 100 nt downstream of the start codon. Alternatively, 5′ or 3′ UTRs and regions nearby the start codon can be used but are generally avoided, as these may be richer in regulatory protein binding sites. UTR-binding proteins andlor translation initiation complexes may interfere with binding of the siRNP or RISC endonuclease complex. An initial BLAST homology search for the selected siRNA sequence is done against an available nucleotide sequence library to ensure that only one gene is targeted. Specificity of target recognition by siRNA duplexes indicate that a single point mutation located in the paired region of an siRNA duplex is sufficient to abolish target mRNA degradation. See, Elbashir et al. 2001 EMBO J. 20(23):6877-88. Hence, consideration should be taken to accommodate SNPs, polymorphisms, allelic variants or species-specific variations when targeting a desired gene.


In one embodiment, a complete NOVX siRNA experiment includes the proper negative control. A negative control siRNA generally has the same nucleotide composition as the NOVX siRNA but lack significant sequence homology to the genome. Typically, one would scramble the nucleotide sequence of the NOVX siRNA and do a homology search to make sure it lacks homology to any other gene.


Two independent NOVX siRNA duplexes can be used to knock-down a target NOVX gene. This helps to control for specificity of the silencing effect. In addition, expression of two independent genes can be simultaneously knocked down by using equal concentrations of different NOVX siRNA duplexes, e.g., a NOVX siRNA and an siRNA for a regulator of a NOVX gene or polypeptide. Availability of siRNA-associating proteins is believed to be more limiting than target mRNA accessibility.


A targeted NOVX region is typically a sequence of two adenines (AA) and two thymidines (TT) divided by a spacer region of nineteen (N19) residues (e.g., AA(N19)TT). A desirable spacer region has a G/C-content of approximately 30% to 70%, and more preferably of about 50%. If the sequence AA(N19)TT is not present in the target sequence, an alternative target region would be AA(N21). The sequence of the NOVX sense siRNA corresponds to (N19)TT or N21, respectively. In the latter case, conversion of the 3′ end of the sense siRNA to TT can be performed if such a sequence does not naturally occur in the NOVX polynucleotide. The rationale for this sequence conversion is to generate a symmetric duplex with respect to the sequence composition of the sense and antisense 3′ overhangs. Symmetric 3′ overhangs may help to ensure that the siRNPs are formed with approximately equal ratios of sense and antisense target RNA-cleaving siRNPs. See, e.g., Elbashir, Lendeckel and Tuschl (2001). Genes & Dev. 15: 188-200, incorporated by reference herein in its entirely. The modification of the overhang of the sense sequence of the siRNA duplex is not expected to affect targeted mRNA recognition, as the antisense siRNA strand guides target recognition.


Alternatively, if the NOVX target mRNA does not contain a suitable AA(N21) sequence, one may search for the sequence NA(N21). Further, the sequence of the sense strand and antisense strand may still be synthesized as 5′ (N19)TT, as it is believed that the sequence of the 3′-most nucleotide of the antisense siRNA does not contribute to specificity. Unlike antisense or ribozyme technology, the secondary structure of the target rnRNA does not appear to have a strong effect on silencing. See, Harborth, et al. (2001) J. Cell Science 114: 4557-4565, incorporated by reference in its entirety.


Transfection of NOVX siRNA duplexes can be achieved using standard nucleic acid transfection methods, for example, OLIGOFECTAMINE Reagent (commercially available from Invitrogen). An assay for NOVX gene silencing is generally performed approximately 2 days after transfection. No NOVX gene silencing has been observed in the absence of transfection reagent, allowing for a comparative analysis of the wild-type and silenced NOVX phenotypes. In a specific embodiment, for one well of a 24-well plate, approximately 0.84 μg of the siRNA duplex is generally sufficient. Cells are typically seeded the previous day, and are transfected at about 50% confluence. The choice of cell culture media and conditions are routine to those of skill in the art, and will vary with the choice of cell type. The efficiency of transfection may depend on the cell type, but also on the passage number and the confluency of the cells. The time and the manner of formation of siRNA-liposome complexes (e.g. inversion versus vortexing) are also critical. Low transfection efficiencies are the most frequent cause of unsuccessful NOVX silencing. The efficiency of transfection needs to be carefully examined for each new cell line to be used. Preferred cell are derived from a mammal, more preferably from a rodent such as a rat or mouse, and most preferably from a human. Where used for therapeutic treatment, the cells are preferentially autologous, although non-autologous cell sources are also contemplated as within the scope of the present invention.


For a control experiment, transfection of 0.84 μg single-stranded sense NOVX siRNA will have no effect on NOVX silencing, and 0.84 μg antisense siRNA has a weak silencing effect when compared to 0.84 μg of duplex siRNAs. Control experiments again allow for a comparative analysis of the wild-type and silenced NOVX phenotypes. To control for transfection efficiency, targeting of common proteins is typically performed, for example targeting of lamin A/C or transfection of a CMV-driven EGFP-expression plasmid (e.g. commercially available from Clontech). In the above example, a determination of the fraction of lamin A/C knockdown in cells is determined the next day by such techniques as immunofluorescence, Western blot, Northern blot or other similar assays for protein expression or gene expression. Lamin A/C monoclonal antibodies may be obtained from Santa Cruz Biotechnology.


Depending on the abundance and the half life (or turnover) of the targeted NOVX polynucleotide in a cell, a knock-down phenotype may become apparent after 1 to 3 days, or even later. In cases where no NOVX knock-down phenotype is observed, depletion of the NOVX polynucleotide may be observed by immunofluorescence or Western blotting. If the NOVX polynucleotide is still abundant after 3 days, cells need to be split and transferred to a fresh 24-well plate for re-transfection. If no knock-down of the targeted protein is observed, it may be desirable to analyze whether the target mRNA (NOVX or a NOVX upstream or downstream gene) was effectively destroyed by the transfected siRNA duplex. Two days after transfection, total RNA is prepared, reverse transcribed using a target-specific primer, and PCR-amplified with a primer pair covering at least one exon-exon junction in order to control for amplification of pre-mRNAs. RT/PCR of a non-targeted mRNA is also needed as control. Effective depletion of the mRNA yet undetectable reduction of target protein may indicate that a large reservoir of stable NOVX protein may exist in the cell. Multiple transfection in sufficiently long intervals may be necessary until the target protein is finally depleted to a point where a phenotype may become apparent. If multiple transfection steps are required, cells are split 2 to 3 days after transfection. The cells may be transfected immediately after splitting.


An inventive therapeutic method of the invention contemplates administering a NOVX siRNA construct as therapy to compensate for increased or aberrant NOVX expression or activity. The NOVX ribopolynucleotide is obtained and processed into siRNA fragments, or a NOVX siRNA is synthesized, as described above. The NOVX siRNA is administered to cells or tissues using known nucleic acid transfection techniques, as described above. A NOVX siRNA specific for a NOVX gene will decrease or knockdown NOVX transcription products, which will lead to reduced NOVX polypeptide production, resulting in reduced NOVX polypeptide activity in the cells or tissues.


The present invention also encompasses a method of treating a disease or condition associated with the presence of a NOVX protein in an individual comprising administering to the individual an RNAi construct that targets the mRNA of the protein (the mRNA that encodes the protein) for degradation. A specific RNAi construct includes a siRNA or a double stranded gene transcript that is processed into siRNAs. Upon treatment, the target protein is not produced or is not produced to the extent it would be in the absence of the treatment.


Where the NOVX gene function is not correlated with a known phenotype, a control sample of cells or tissues from healthy individuals provides a reference standard for determining NOVX expression levels. Expression levels are detected using the assays described, e.g., RT-PCR, Northern blotting, Western blotting, ELISA, and the like. A subject sample of cells or tissues is taken from a mammal, preferably a human subject, suffering from a disease state. The NOVX ribopolynucleotide is used to produce siRNA constructs, that are specific for the NOVX gene product. These cells or tissues are treated by administering NOVX siRNA's to the cells or tissues by methods described for the transfection of nucleic acids into a cell or tissue, and a change in NOVX polypeptide or polynucleotide expression is observed in the subject sample relative to the control sample, using the assays described. This NOVX gene knockdown approach provides a rapid method for determination of a NOVX minus (NOVX−) phenotype in the treated subject sample. The NOVX- phenotype observed in the treated subject sample thus serves as a marker for monitoring the course of a disease state during treatment.


In specific embodiments, a NOVX siRNA is used in therapy. Methods for the generation and use of a NOVX siRNA are known to those skilled in the art. Example techniques are provided below.


Production of RNAs


Sense RNA (ssRNA) and antisense RNA (asRNA) of NOVX are produced using known methods such as transcription in RNA expression vectors. In the initial experiments, the sense and antisense RNA are about 500 bases in length each. The produced ssRNA and asRNA (0.5 μM) in 10 mM Tris-HCl (pH 7.5) with 20 mM NaCl were heated to 95° C. for 1 min then cooled and annealed at room temperature for 12 to 16 h. The RNAs are precipitated and resuspended in lysis buffer (below). To monitor annealing, RNAs are electrophoresed in a 2% agarose gel in TBE buffer and stained with ethidium bromide. See, e.g., Sambrook et al., Molecular Cloning. Cold Spring Harbor Laboratory Press, Plainview, N.Y. (1989).


Lysate Preparation


Untreated rabbit reticulocyte lysate (Ambion) are assembled according to the manufacturer's directions. dsRNA is incubated in the lysate at 30° C. for 10 min prior to the addition of mRNAs. Then NOVX mRNAs are added and the incubation continued for an additional 60 min. The molar ratio of double stranded RNA and MrRNA is about 200:1. The NOVX mRNA is radiolabeled (using known techniques) and its stability is monitored by gel electrophoresis.


In a parallel experiment made with the same conditions, the double stranded RNA is internally radiolabeled with a 32P-ATP. Reactions are stopped by the addition of 2× proteinase K buffer and deproteinized as described previously (Tuschl et al., Genes Dev., 13:3191-3197 (1999)). Products are analyzed by electrophoresis in 15% or 18% polyacrylamide sequencing gels using appropriateRNA standards. By monitoring the gels for radioactivity, the natural production of 10 to 25 nt RNAs from the double stranded RNA can be determined.


The band of double stranded RNA, about 21-23 bps, is eluded. The efficacy of these 21-23 mers for suppressing NOVX transcription is assayed in vitro using the same rabbit reticulocyte assay described above using 50 nanomolar of double stranded 21-23 mer for each assay. The sequence of these 21-23 mers is then determined using standard nucleic acid sequencing techniques.


RNA Preparation 21 nt RNAs, based on the sequence determined above, are chemically synthesized using Expedite RNA phosphoramidites and thymidine phosphoramidite (Proligo, Germany). Synthetic oligonucleotides are deprotected and gel-purified (Elbashir, Lendeckel, & Tuschl, Genes & Dev. 15, 188-200 (2001)), followed by Sep-Pak C18 cartridge (Waters, Milford, Mass., USA) purification (Tuschl, et al., Biochemistry, 32:11658-11668 (1993)).


These RNAs (20 μM) single strands are incubated in annealing buffer (100 mM potassium acetate, 30 mM HEPES-KOH at pH 7.4, 2 mM magnesium acetate) for 1 min at 90° C. followed by 1 h at 37° C.


Cell Culture


A cell culture known in the art to regularly express NOVX is propagated using standard conditions. 24 hours before transfection, at approx. 80% confluency, the cells are trypsinized and diluted 1:5 with fresh medium without antibiotics (1-3×105 cells/ml) and transferred to 24-well plates (500 ml/well). Transfection is performed using a commercially available lipofection kit and NOVX expression is monitored using standard techniques with positive and negative control. A positive control is cells that naturally express NOVX while a negative control is cells that do not express NOVX. Base-paired 21 and 22 nt siRNAs with overhanging 3′ ends mediate efficient sequence-specific mRNA degradation in lysates and in cell culture. Different concentrations of siRNAs are used. An efficient concentration for suppression in vitro in mamrnmalian culture is between 25 nM to 100 nM final concentration. This indicates that siRNAs are effective at concentrations that are several orders of magnitude below the concentrations applied in conventional antisense or ribozyme gene targeting experiments.


The above method provides a way both for the deduction of NOVX siRNA sequence and the use of such siRNA for in vitro suppression. In vivo suppression may be performed using the same siRNA using well known in vivo transfection or gene therapy transfection techniques.


Antisense Nucleic Acids


Another aspect of the invention pertains to isolated antisense nucleic acid molecules that are hybridizable to or complementary to the nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or fragments, analogs or derivatives thereof. An “antisense” nucleic acid comprises a nucleotide sequence that is complementary to a “sense” nucleic acid encoding a protein (e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA sequence). In specific aspects, antisense nucleic acid molecules are provided that comprise a sequence complementary to at least about 10, 25, 50, 100, 250 or 500 nucleotides or an entire NOVX coding strand, or to only a portion thereof. Nucleic acid molecules encoding fragments, homologs, derivatives and analogs of a NOVX protein of SEQ ID NO:2n, wherein n is an integer between 1 and 606, or antisense nucleic acids complementary to a NOVX nucleic acid sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, are additionally provided.


In one embodiment, an antisense nucleic acid molecule is antisense to a “coding region” of the coding strand of a nucleotide sequence encoding a NOVX protein. The term “coding region” refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues. In another embodiment, the antisense nucleic acid molecule is antisense to a “noncoding region” of the coding strand of a nucleotide sequence encoding the NOVX protein. The term “noncoding region” refers to 5′ and 3′ sequences which flank the coding region that are not translated into amino acids (i.e., also referred to as 5′ and 3′ untranslated regions).


Given the coding strand sequences encoding the NOVX protein disclosed herein, antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick or Hoogsteen base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of NOVX mRNA, but more preferably is an oligonucleotide that is antisense to only a portion of the coding or noncoding region of NOVX mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of NOVX mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis or enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid (e.g., an antisense oligonucleotide) can be chemically synthesized using naturally-occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids (e.g., phosphorothioate derivatives and acridine substituted nucleotides can be used).


Examples of modified nucleotides that can be used to generate the antisense nucleic acid include: 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5-carboxymethylaminomethyl-2-thiouridine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 5-methoxyuracil, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil, 2-thiouracil, 4-thiouracil, beta-D-mannosylqueosine, 5′-methoxycarboxymethyluracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).


The antisense nucleic acid molecules of the invention are typically administered to a subject or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encodinga NOVX protein to thereby inhibit expression of the protein (e.g., by inhibiting transcription and/or translation). The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule that binds to DNA duplexes, through specific interactions in the major groove of the double helix. An example of a route of administration of antisense nucleic acid molecules of the invention includes direct injection at a tissue site. Alternatively, antisense nucleic acid molecules can be modified to target selected cells and then administered systemically. For example, for systemic administration, antisense molecules can be modified such that they specifically bind to receptors or antigens expressed on a selected cell surface (e.g., by linking the antisense nucleic acid molecules to peptides or antibodies that bind to cell surface receptors or antigens). The antisense nucleic acid molecules can also be delivered to cells using the vectors described herein. To achieve sufficient nucleic acid molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong pol II or pol III promoter are preferred.


In yet another embodiment, the antisense nucleic acid molecule of the invention is an α-anomeric nucleic acid molecule. An α-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual β-units, the strands run parallel to each other. See, e.g., Gaultier, et al., 1987. Nucl. Acids Res. 15: 6625-6641. The antisense nucleic acid molecule can also comprise a 2′-o-methylribonucleotide (See, e.g., Inoue, et al. 1987. Nucl. Acids Res. 15: 6131-6148) or a chimeric RNA-DNA analogue (See, e.g., Inoue, et al., 1987. FEBS Lett. 215: 327-330.


Ribozymes and PNA Moieties


Nucleic acid modifications include, by way of non-limiting example, modified bases, and nucleic acids whose sugar phosphate backbones are modified or derivatized. These modifications are carried out at least in part to enhance the chemical stability of the modified nucleic acid, such that they may be used, for example, as antisense binding nucleic acids in therapeutic applications in a subject.


In one embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity that are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes (e.g., hammerhead ribozymes as described in Haselhoff and Gerlach 1988. Nature 334: 585-591) can be used to catalytically cleave NOVX mRNA transcripts to thereby inhibit translation of NOVX mRNA. A ribozyme having specificity for a NOVX-encoding nucleic acid can be designed based upon the nucleotide sequence of a NOVX cDNA disclosed herein (i.e., SEQ ID NO:2n−1, wherein n is an integer between 1 and 606). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in a NOVX-encoding mRNA. See, e.g., U.S. Pat. No. 4,987,07to Cech, et al. and U.S. Pat. No. 5,116,742 to Cech, et al. NOVX mRNA can also be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, e.g., Bartel et al., (1993) Science 261:1411-1418.


Alternatively, NOVX gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of the NOVX nucleic acid (e.g., the NOVX promoter and/or enhancers) to form triple helical structures that prevent transcription of the NOVX gene in target cells. See, e.g., Helene, 1991. Anticancer Drug Des. 6: 569-84; Helene, et al. 1992. Ann. N.Y. Acad. Sci. 660: 27-36; Maher, 1992. Bioassays 14: 807-15.


In various embodiments, the NOVX nucleic acids can be modified at the base moiety, sugar moiety or phosphate backbone to improve, e.g., the stability, hybridization, or solubility of the molecule. For example, the deoxyribose phosphate backbone of the nucleic acids can be modified to generate peptide nucleic acids. See, e.g., Hyrup, et al., 1996. Bioorg Med Chem 4: 5-23. As used herein, the terms “peptide nucleic acids” or “PNAs” refer to nucleic acid mimics (e.g., DNA mimics) in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleotide bases are retained. The neutral backbone of PNAs has been shown to allow for specific hybridization to DNA and RNA under conditions of low ionic strength. The synthesis of PNA oligomer can be performed using standard solid phase peptide synthesis protocols as described in Hyrup, et al., 1996. supra; Perry-O'Keefe, et al., 1996. Proc. Natl. Acad. Sci. USA 93: 14670-14675.


PNAs of NOVX can be used in therapeutic and diagnostic applications. For example, PNAs can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, e.g., inducing transcription or translation arrest or inhibiting replication. PNAs of NOVX can also be used, for example, in the analysis of single base pair mutations in a gene (e.g., PNA directed PCR clamping; as artificial restriction enzymes when used in combination with other enzymes, e.g., S1 nucleases (See, Hyrup, et al., 1996.supra); or as probes or primers for DNA sequence and hybridization (See, Hyrup, et al., 1996, supra; Perry-O'Keefe, et al., 1996. supra).


In another embodiment, PNAs of NOVX can be modified, e.g., to enhance their stability or cellular uptake, by attaching lipophilic or other helper groups to PNA, by the formation of PNA-DNA chimeras, or by the use of liposomes or other techniques of drug delivery known in the art. nFor example, PNA-DNA chimeras of NOVX can be generated that may combine the advantageous properties of PNA and DNA. Such chimeras allow DNA recognition enzymes (e.g., RNase H and DNA polymerases) to interact with the DNA portion while the PNA portion would provide high binding affinity and specificity. PNA-DNA chimeras can be linked using linkers of appropriate lengths selected in terms of base stacking, number of bonds between the nucleotide bases, and orientation (see, Hyrup, et al., 1996. supra). The synthesis of PNA-DNA chimeras can be performed as described in Hyrup, et al., 1996. supra and Finn, et al., 1996. Nucl Acids Res 24: 3357-3363. For example, a DNA chain can be synthesized on a solid support using standard phosphoramidite coupling chemistry, and modified nucleoside analogs, e.g., 5′-(4-methoxytrityl)amino-5′-deoxy-thymidine phosphoramidite, can be used between the PNA and the 5′ end of DNA. See, e.g., Mag, et al., 1989. Nucl Acid Res 17: 5973-5988. PNA monomers are then coupled in a stepwise manner to produce a chimeric molecule with a 5′ PNA segment and a 3′ DNA segment. See, e.g., Finn, et al., 1996. supra. Alternatively, chimeric molecules can be synthesized with a 5′ DNA segment and a 3′ PNA segment. See, e.g., Petersen, et al., 1975. Bioorg. Med. Chem. Lett. 5: 1119-11124.


In other embodiments, the oligonucleotide may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo), or agents facilitating transport across the cell membrane (see, e.g., Letsinger, et al., 1989. Proc. Natl. Acad. Sci. U.S.A. 86: 6553-6556; Lemaitre, et al., 1987. Proc. Natl. Acad. Sci. 84: 648-652; PCT Publication No. WO88/09810) or the blood-brain barrier (see, e.g., PCT Publication No. WO 89/10134). In addition, oligonucleotides can be modified with hybridization triggered cleavage agents (see, e.g., Krol, et al., 1988. BioTechniques 6:958-976) or intercalating agents (see, e.g., Zon, 1988. Pharm. Res. 5: 539-549). To this end, the oligonucleotide may be conjugated to another molecule, e.g., a peptide, a hybridization triggered cross-linking agent, a transport agent, a hybridization-triggered cleavage agent, and the like.


NOVX Polypeptides


A polypeptide according to the invention includes a polypeptide including the amino acid sequence of NOVX polypeptides whose sequences are provided in any one of SEQ ID NO:2n, wherein n is an integer between 1 and 606. The invention also includes a mutant or variant protein any of whose residues may be changed from the corresponding residues shown in any one of SEQ ID NO:2n, wherein n is an integer between 1 and 606, while still encoding a protein that maintains its NOVX activities and physiological functions, or a functional fragment thereof.


In general, a NOVX variant that preserves NOVX-like function includes any variant in which residues at a particular position in the sequence have been substituted by other amino acids, and further include the possibility of inserting an additional residue or residues between two residues of the parent protein as well as the possibility of deleting one or more residues from the parent sequence. Any amino acid substitution, insertion, or deletion is encompassed by the invention. In favorable circumstances, the substitution is a conservative substitution as defined above.


One aspect of the invention pertains to isolated NOVX proteins, and biologically-active portions thereof, or derivatives, fragments, analogs or homologs thereof. Also provided are polypeptide fragments suitable for use as immunogens to raise anti-NOVX antibodies. In one embodiment, native NOVX proteins can be isolated from cells or tissue sources by an appropriate purification scheme using standard protein purification techniques. In another embodiment, NOVX proteins are produced by recombinant DNA techniques. Alternative to recombinant expression, a NOVX protein or polypeptide can be synthesized chemically using standard peptide synthesis techniques.


An “isolated” or “purified” polypeptide or protein or biologically-active portion thereof is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the NOVX protein is derived, or substantially free from chemical precursors or other chemicals when chemically synthesized. The language “substantially free of cellular material” includes preparations of NOVX proteins in which the protein is separated from cellular components of the cells from which it is isolated or recombinantly-produced. In one embodiment, the language “substantially free of cellular material” includes preparations of NOVX proteins having less than about 30% (by dry weight) of non-NOVX proteins (also referred to herein as a “contaminating protein”), more preferably less than about 20% of non-NOVX proteins, still more preferably less than about 10% of non-NOVX proteins, and most preferably less than about 5% of non-NOVX proteins. When the NOVX protein or biologically-active portion thereof is recombinantly-produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the NOVX protein preparation.


The language “substantially free of chemical precursors or other chemicals” includes preparations of NOVX proteins in which the protein is separated from chemical precursors or other-chemicals that are involved in the synthesis of the protein. In one embodiment, the language “substantially free of chemical precursors or other chemicals” includes preparations of NOVX proteins having less than about 30% (by dry weight) of chemical precursors or non-NOVX chemicals, more preferably less than about 20% chemical precursors or non-NOVX chemicals, still more preferably less than about 10% chemical precursors or non-NOVX chemicals, and most preferably less than about 5% chemical precursors or non-NOVX chemicals.


Biologically-active portions of NOVX proteins include peptides comprising amino acid sequences sufficiently homologous to or derived from the amino acid sequences of the NOVX proteins (e.g., the amino acid sequence of SEQ ID NO:2n, wherein n is an integer between 1 and 606) that include fewer amino acids than the full-length NOVX proteins, and exhibit at least one activity of a NOVX protein. Typically, biologically-active portions comprise a domain or motif with at least one activity of the NOVX protein. A biologically-active portion of a NOVX protein can be a polypeptide which is, for example, 10, 25, 50, 100 or more amino acid residues in length.


Moreover, other biologically-active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the functional activities of a native NOVX protein.


In an embodiment, the NOVX protein has an amino acid sequence of SEQ ID NO:2n, wherein n is an integer between 1 and 606. In other embodiments, the NOVX protein is substantially homologous to SEQ ID NO:2n, wherein n is an integer between 1 and 606, and retains the functional activity of the protein of SEQ ID NO:2n, wherein n is an integer between 1 and 606, yet differs in amino acid sequence due to natural allelic variation or mutagenesis, as described in detail, below. Accordingly, in another embodiment, the NOVX protein is a protein that comprises an amino acid sequence at least about 45% homologous to the amino acid sequence of SEQ ID NO:2n, wherein n is an integer between 1 and 606, and retains the functional activity of the NOVX proteins of SEQ ID NO:2n, wherein n is an integer between 1 and 606.


Determining Homology Between Two or More Sequences


To determine the percent homology of two amino acid sequences or of two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of a first amino acid or nucleic acid sequence for optimal alignmentwith a second amino or nucleic acid sequence). The amino acid residues or nucleotides at corresponding amino acid positions ornucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are homologous at that position (i.e., as used herein amino acid or nucleic acid “homology” is equivalent to amino acid or nucleic acid “identity”).


The nucleic acid sequence homology may be determined as the degree of identity between two sequences. The homology may be determined using computer programs known in the art, such as GAP software provided in the GCG program package. See, Needleman and Wunsch, 1970. J Mol Biol 48: 443-453. Using GCG GAP software with the following settings for nucleic acid sequence comparison: GAP creation penalty of 5.0 and GAP extension penalty of 0.3, the coding region of the analogous nucleic acid sequences referred to above exhibits a degree of identity preferably of at least 70%, 75%, 80%, 85%, 90%, 95%, 98%, or 99%, with the CDS (encoding) part of the DNA sequence of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606.


The term “sequence identity” refers to the degree to which two polynucleotide or polypeptide sequences are identical on a residue-by-residue basis over a particular region of comparison. The term “percentage of sequence identity” is calculated by comparing two optimally aligned sequences over that region of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G. U, or I, in the case of nucleic acids) occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the region of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. The term “substantial identity” as used herein denotes a characteristic of a polynucleotide sequence, wherein the polynucleotide comprises a sequence that has at least 80 percent sequence identity, preferably at least 85 percent identity and often 90 to 95 percent sequence identity, more usually at least 99 percent sequence identity as compared to a reference sequence over a comparison region.


Chimeric and Fusion Proteins


The invention also provides NOVX chimeric or fusion proteins. As used herein, a NOVX “chimeric protein” or “fusion protein” comprises a NOVX polypeptide operatively-linked to a non-NOVX polypeptide. An “NOVX polypeptide” refers to a polypeptide having an amino acid sequence corresponding to a NOVX protein of SEQ ID NO:2n, wherein n is an integer between 1 and 606, whereas a “non-NOVX polypeptide” refers to a polypeptide having an amino acid sequence corresponding to a protein that is not substantially homologous to the NOVX protein, e.g., a protein that is different from the NOVX protein and that is derived from the same or a different organism. Within a NOVX fusion protein the NOVX polypeptide can correspond to all or a portion of a NOVX protein. In one embodiment, a NOVX fusion protein comprises at least one biologically-active portion of a NOVX protein. In another embodiment, a NOVX fusion protein comprises at least two biologically-active portions of a NOVX protein. In yet another embodiment, a NOVX fusion protein comprises at least three biologically-active portions of a NOVX protein. Within the fusion protein, the term “operatively-linked” is intended to indicate that the NOVX polypeptide and the non-NOVX polypeptide are fused in-frame with one another. The non-NOVX polypeptide can be fused to the N-terminus or C-terminus of the NOVX polypeptide.


In one embodiment, the fusion protein is a GST-NOVX fusion protein in which the NOVX sequences are fused to the C-terminus of the GST (glutathione S-transferase) sequences. Such fusion proteins can facilitate the purification of recombinant NOVX polypeptides.


In another embodiment, the fusion protein is a NOVX protein containing a heterologous signal sequence at its N-terminus. In certain host cells (e.g., mammalian host cells), expression and/or secretion of NOVX can be increased through use of a heterologous signal sequence.


In yet another embodiment, the fusion protein is a NOVX-immunoglobulin fusion protein in which the NOVX sequences are fused to sequences derived from a member of the immunoglobulin protein family. The NOVX-immunoglobulin fusion proteins of the invention can be incorporated into pharmaceutical compositions and administered to a subject to inhibit an interaction between a NOVX ligand and a NOVX protein on the surface of a cell, to thereby suppress NOVX-mediated signal transduction in vivo. The NOVX-immunoglobulin fusion proteins can be used to affect the bioavailability of a NOVX cognate ligand. Inhibition of the NOVX ligand/NOVX interaction may be useful therapeutically for both the treatment of proliferative and differentiative disorders, as well as modulating (e.g. promoting or inhibiting) cell survival. Moreover, the NOVX-immunoglobulin fusion proteins of the invention can be used as immunogens to produce anti-NOVX antibodies in a subject, to purify NOVX ligands, and in screening assays to identify molecules that inhibit the interaction of NOVX with a NOVX ligand.


A NOVX chimeric or fusion protein of the invention can be produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, e.g., by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of gene fragments can be carried out using anchor primers that give rise to complementary overhangs between two consecutive gene fragments that can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, e.g., Ausubel, et al. (eds.) CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, John Wiley & Sons, 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST polypeptide). A NOVX-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the NOVX protein.


NOVX Agonists and Antagonists


The invention also pertains to variants of the NOVX proteins that function as either NOVX agonists (i.e., mimetics) or as NOVX antagonists. Variants of the NOVX protein can be generated by mutagenesis (e.g., discrete point mutation or truncation of the NOVX protein). An agonist of the NOVX protein can retain substantially the same, or a subset of, the biological activities of the naturally occurring form of the NOVX protein. An antagonist of the NOVX protein can inhibit one or more of the activities of the naturally occurring form of the NOVX protein by, for example, competitively binding to a downstream or upstream member of a cellular signaling cascade which includes the NOVX protein. Thus, specific biological effects can be elicited by treatment with a variant of limited function. In one embodiment, treatment of a subject with a variant having a subset of the biological activities of the naturally occurring form of the protein has fewer side effects in a subject relative to treatment with the naturally occurring form of the NOVX proteins.


Variants of the NOVX proteins that function as either NOVX agonists (ie., mimetics) or as NOVX antagonists can be identified by screening combinatorial libraries of mutants (e.g., truncation mutants) of the NOVX proteins for NOVX protein agonist or antagonist activity. In one embodiment, a variegated library of NOVX variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of NOVX variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential NOVX sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins (e.g., for phage display) containing the set of NOVX sequences therein. There are a variety of methods which can be used to produce libraries of potential NOVX variants from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential NOVX sequences. Methods for synthesizing degenerate oligonucleotides are well-known within the art. See, e.g., Narang, 1983. Tetrahedron 39: 3; Itakura, et al., 1984. Annu. Rev. Biochem. 53: 323; Itakura, et al., 1984. Science 198: 1056; Ike, et al., 1983. Nucl. Acids Res. 11: 477.


Polypeptide Libraries


In addition, libraries of fragments of the NOVX protein coding sequences can be used to generate a variegated population of NOVX fragments for screening and subsequent selection of variants of a NOVX protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of a NOVX coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double-stranded DNA that can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with SI nuclease, and ligating the resulting fragment library into an expression vector. By this method, expression libraries can be derived which encodes N-terminal and internal fragments of various sizes of the NOVX proteins.


Various techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of NOVX proteins. The most widely used techniques, which are amenable to high throughput analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique that enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify NOVX variants. See, e.g., Arkin and Yourvan, 1992. Proc. Natl. Acad. Sci. USA 89: 7811-7815; Delgrave, et al., 1993. Protein Engineering 6:327-331.


Anti-NOVX Antibodies


Included in the invention are antibodies to NOVX proteins, or fragments of NOVX proteins. The term “antibody” as used herein refers to immunoglobulin molecules and immunologically active portions of immunoglobulin (Ig) molecules, i.e., molecules that contain an antigen binding site that specifically binds (immunoreacts with) an antigen. Such antibodies include, but are not limited to, polyclonal, monoclonal, chimeric, single chain, Fab, Fab′ and F(ab′)2 fragments, and an Fab expression library. In general, antibody molecules obtained from humans relates to any of the classes IgG, IgM, IgA, IgE and IgD, which differ from one another by the nature of the heavy chain present in the molecule. Certain classes have subclasses as well, such as IgG1, IgG2, and others. Furthermore, in humans, the light chain may be a kappa chain or a lambda chain. Reference herein to antibodies includes a reference to all such classes, subclasses and types of human antibody species.


An isolated protein of the invention intended to serve as an antigen, or a portion or fragment thereof, can be used as an immunogen to generate antibodies that immunospecifically bind the antigen, using standard techniques for polyclonal and monoclonal antibody preparation. The full-length protein can be used or, alternatively, the ! invention provides antigenic peptide fragments of the antigen for use as immunogens. An antigenic peptide fragment comprises at least 6 amino acid residues of the amino acid sequence of the full length protein, such as an amino acid sequence of SEQ ID NO:2n, wherein n is an integer between 1 and 606, and encompasses an epitope thereof such that X an antibody raised against the peptide forms a specific immune complex with the full length protein or with any fragment that contains the epitope. Preferably, the antigenic peptide comprises at least 10 amino acid residues, or at least 15 amino acid residues, or at least 20 amino acid residues, or at least 30 amino acid residues. Preferred epitopes encompassed by the antigenic peptide are regions of the protein that are located on its surface; commonly these are hydrophilic regions.


In certain embodiments of the invention, at least one epitope encompassed by the antigenic peptide is a region of NOVX that is located on the surface of the protein, e.g., a hydrophilic region. A hydrophobicity analysis of the human NOVX protein sequence will indicate which regions of a NOVX polypeptide are particularly hydrophilic and, therefore, are likely to encode surface residues useful for targeting antibody production. As a means for targeting antibody production, hydropathy plots showing regions of hydrophilicity and hydrophobicity may be generated by any method well known in the art, including, for example, the Kyte Doolittle or the Hopp Woods methods, either with or without Fourier transformation. See, e.g., Hopp and Woods, 1981, Proc. Nat. Acad. Sci. USA 78: 3824-3828; Kyte and Doolittle 1982, J. Mol. Biol. 157: 105-142, each incorporated herein by reference in their entirety. Antibodies that are specific for one or more domains within an antigenic protein, or derivatives, fragments, analogs or homologs thereof, are also provided herein.


I The term “epitope” includes any protein determinant capable of specific binding to an immunoglobulin or T-cell receptor. Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains and usually have specific three dimensional structural characteristics, as well as specific charge characteristics. A NOVX polypeptide or a fragment thereof comprises at least one antigenic epitope. An anti-NOVX antibody of the present invention is said to specifically bind to antigen NOVX when the equilibrium binding constant (KD) is ≦1 μM, preferably ≦100 nM, more preferably ≦10 nM, and most preferably ≦100 pM to about 1 pM, as measured by assays including radioligand binding assays or similar assays known to skilled artisans.


A protein of the invention, or a derivative, fragment, analog, homolog or ortholog thereof, may be utilized as an immunogen in the generation of antibodies that immunospecifically bind these protein components.


Various procedures known within the art may be used for the production of polyclonal or monoclonal antibodies directed against a protein of the invention, or against derivatives, fragments, analogs homologs or orthologs thereof (see, for example, Antibodies: A Laboratory Manual, Harlow E, and Lane D, 1988, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., incorporated herein by reference). Some of these antibodies are discussed below.


Polyclonal Antibodies


For the production of polyclonal antibodies, various suitable host animals (e.g., rabbit, goat, mouse or other mammal) may be immunized by one or more injections with the native protein, a synthetic variant thereof, or a derivative of the foregoing. An appropriate immunogenic preparation can contain, for example, the naturally occurring immunogenic protein, a chemically synthesized polypeptide representing the immunogenic protein, or a recombinantly expressed immunogenic protein. Furthermore, the protein may be conjugated to a second protein known to be immunogenic in the mammal being immunized. Examples of such immunogenic proteins include but are not limited to keyhole limpet hemocyanin, serum albumin, bovine thyroglobulin, and soybean trypsin inhibitor. The preparation can further include an adjuvant. Various adjuvants used to increase the immunological response include, but are not limited to, Freund's (complete and incomplete), mineral gels (e.g., aluminum hydroxide), surface active substances (e.g., lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, dinitrophenbl, etc.), adjuvants usable in humans such as Bacille Calmette-Guerin and Corynebacterium parvum, or similar immunostimulatory agents. Additional examples of adjuvants which can be employed include MPL-TDM adjuvant (monophosphoryl Lipid A, synthetic trehalose dicorynomycolate).


The polyclonal antibody molecules directed against the immunogenic protein can be isolated from the mammal (e.g., from the blood) and further purified by well known techniques, such as affinity chromatography using protein A or protein G, which provide primarily the IgG fraction of immune serum. Subsequently, or alternatively, the specific antigen which is the target of the immunoglobulin sought, or an epitope thereof, may be immobilized on a column to purify the immune specific antibody by immunoaffinity chromatography. Purification of immunoglobulins is discussed, for example, by D. Wilkinson (The Scientist, published by The Scientist, Inc., Philadelphia Pa., Vol. 14, No. 8 (Apr. 17, 2000), pp. 25-28).


Monoclonal Antibodies


The term “monoclonal antibody” (MAb) or “monoclonal antibody composition”, as used herein, refers to a population of antibody molecules that contain only one molecular species of antibody molecule consisting of a unique light chain gene product and a unique heavy chain gene product. In particular, the complementarity determining regions (CDRs) of the monoclonal antibody are identical in all the molecules of the population. MAbs thus contain an antigen binding site capable of immunoreacting with a particular epitope of the antigen characterized by a unique binding affinity for it.


Monoclonal antibodies can be prepared using hybridoma methods, such as those described by Kohler and Milstein, Nature, 256:495 (1975). In a hybridoma method, a mouse, hamster, or other appropriate host animal, is typically immunized with an immunizing agent to elicit lymphocytes that produce or are capable of producing antibodies that will specifically bind to the immunizing agent. Alternatively, the lymphocytes can be immunized in vitro.


The immunizing agent will typically include the protein antigen, a fragment thereof or a fusion protein thereof. Generally, either peripheral blood lymphocytes are used if cells of human origin are desired, or spleen cells-or lymph node cells are used if non-human mammalian sources are desired. The lymphocytes are then fused with an immortalized cell line using a suitable fusing agent, such as polyethylene glycol, to form a hybridoma cell (Goding, Monoclonal Antibodies: Principles and Practice, Academic Press, (1986) pp. 59-103). Immortalized cell lines are usually transformed mammalian cells, particularly myeloma cells of rodent, bovine and human origin. Usually, rat or mouse myeloma cell lines are employed. The hybridoma cells can be cultured in a suitable culture medium that preferably contains one or more substances that inhibit the growth or survival of the unfused, immortalized cells. For example, if the parental cells lack the enzyme hypoxanthine guanine phosphoribosyl transferase (HGPRT or HPRT), the culture medium for the hybridomas typically will include hypoxanthine, aminopterin, and thymidine (“HAT medium”), which substances prevent the growth of HGPRT-deficient cells.


Preferred immortalized cell lines are those that fuse efficiently, support stable high level expression of antibody by the selected antibody-producing cells, and are sensitive to a medium such as HAT medium. More preferred immortalized cell lines are murine myeloma lines, which can be obtained, for instance, from the Salk Institute Cell Distribution Center, San Diego, Calif. and the American Type Culture Collection, Manassas, Virginia. Human myeloma and mouse-human heteromyeloma cell lines also have been described for the production of human monoclonal antibodies (Kozbor, J. Immunol., 133:3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, Marcel Dekker, Inc., New York, (1987) pp. 51-63).


The culture medium in which the hybridoma cells are cultured can then be assayed for the presence of monoclonal antibodies directed against the antigen. Preferably, the binding specificity of monoclonal antibodies produced by the hybridoma cells is determined by immunoprecipitation or by an in vitro binding assay, such as radioimmunoassay (RIA) or enzyme-linked immunoabsorbent assay (ELISA). Such techniques and assays are known in the art. The binding affinity of the monoclonal antibody can, for example, be determined by the Scatchard analysis of Munson and Pollard, Anal. Biochem., 107:220 (1980). It is an objective, especially important in therapeutic applications of monoclonal antibodies, to identify antibodies having a high degree of specificity and a high binding affinity for the target antigen.


After the desired hybridoma cells are identified, the clones can be subcloned by limiting dilution procedures and grown by standard methods (Goding,1986). Suitable culture media for this purpose include, for example, Dulbecco's Modified Eagle's Medium and RPMI-1640 medium. Altematively,the hybridoma cells can be grown in vivo as ascites in a mammal.


The monoclonal antibodies secreted by the subclones can be isolated or purified from the culture medium or ascites fluid by conventional immunoglobulin purification procedures such as, for example, protein A-Sepharose, hydroxylapatite chromatography, gel electrophoresis, dialysis, or affinity chromatography.


The monoclonal antibodies can also be made by recombinant DNA methods, such as those described in U.S. Pat. No. 4,816,567. DNA encoding the monoclonal antibodies of the invention can be readily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains of murine antibodies). The hybridoma cells of the invention serve as a preferred source of such DNA. Once isolated, the DNA can be placed into expression vectors, which are then transfected into host cells such as simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, to obtain the synthesis of monoclonal antibodies in the recombinant host cells. The DNA also can be modified, for example, by substituting the coding sequence for human heavy and light chain constant domains in place of the homologous murine sequences (U.S. Pat. No. 4,816,567; Morrison, Nature 368, 812-13 (1994)) or by covalently joining to the immunoglobulin coding sequence all or part of the coding sequence for a non immunoglobulin polypeptide. Such a non-immunoglobulin polypeptide can be substituted for the constant domains of an antibody of the invention, or can be substituted for the variable domains of one antigen-combining site of an antibody of the invention to create a chimeric bivalent antibody.


Humanized Antibodies


The antibodies directed against the protein antigens of the invention can further comprise humanized antibodies or human antibodies. These antibodies are suitable for administration to humans without engendering an immune response by the human against the administered immunoglobulin. Humanized forms of antibodies are chimeric immunoglobulins, immunoglobulin chains or fragments thereof (such as Fv, Fab, Fab′, F(ab′)2 or other antigen-binding subsequences of antibodies) that are principally comprised of the sequence of a human immunoglobulin, and contain minimal sequence derived from a non-human immunoglobulin. Humanization can be performed following the method of Winter and co-workers (Jones et al.;,Nature, 321:522-525 (1986); Riechmann et al., Nature, 332:323-327 (1988); Verhoeyen et al., Science, 239:1534-1536 (1988)), by substituting rodent CDRs or CDR sequences for the corresponding sequences of a human antibody. (See also U.S. Pat. No. 5,225,539.) In some instances, Fv framework residues of the human immunoglobulin are replaced by corresponding non-human residues. Humanized antibodies can also comprise residues which are found neither in the recipient antibody nor in the imported CDR or framework sequences. In general, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the CDR regions correspond to those of a non-human immunoglobulin and all or substantially all of the framework regions are those of a human immunoglobulin consensus sequence. The humanized antibody optimally also will comprise at least a portion of an immunoglobulin constant region (Fc), typically that of a human immunoglobulin (Jones et al., 1986; Riechmnann et al., 1988; and Presta, Curr. Op. Struct. Biol., 2:593-596 (1992)).


Human Antibodies


Fully human antibodies essentially relate to antibody molecules in which the entire sequence of both the light chain and the heavy chain, including the CDRs, arise from human genes. Such antibodies are termed “human antibodies”, or “fully human antibodies” herein. Human monoclonal antibodies can be prepared by the trioma technique; the human B-cell hybridoma technique (see Kozbor, et al., 1983 Immunol Today 4: 72) and the EBV hybridoma technique to produce human monoclonal antibodies (see Cole, et al., 1985 In: MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc., pp. 77-96). Human monoclonal antibodies may be utilized in the practice of the present invention and may be produced by using human hybridomas (see Cote, et al., 1983. Proc Natl Acad Sci USA 80: 2026-2030) or by transforming human B-cells with Epstein Barr Virus in vitro (see Cole, et al., 1985 In: MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc., pp. 77-96).


In addition, human antibodies can also be produced using additional techniques, including phage display libraries (Hoogenboom and Winter, J. Mol. Biol., 227:381 (1991); Marks et al., J. Mol. Biol., 222:581 (1991)). Similarly, human antibodies can be made by introducing human immunoglobulin loci into transgenic animals, e.g., mice in which the endogenous immunoglobulin genes have been partially or completely inactivated. Upon challenge, human antibody production is observed, which closely resembles that seen in humans in all respects, including gene rearrangement, assembly, and antibody repertoire. This approach is described, for example, in U.S. Pat. Nos. 5,545,807; 5,545,806; 5,569,825; 5,625,126; 5,633,425; 5,661,016, and in Marks et al. (Bio/Technology 10, 779-783 (1992)); Lonberg et al. (Nature 368 856-859 (1994)); Morrison ( Nature 368, 812-13 (1994)); Fishwild et al,( Nature Biotechnology 14, 845-51 (1996)); Neuberger (Nature Biotechnology 14, 826 (1996)); and Lonberg and Huszar (Intern. Rev. Immunol. 13 65-93 (1995)).


Human antibodies may additionally be produced using transgenic nonhuman animals which are modified so as to produce fully human antibodies rather than the animal's endogenous antibodies in response to challenge by an antigen. (See PCT publication WO94/02602). The endogenous genes encoding the heavy and light immunoglobulin chains in the nonhuman host have been incapacitated, and active loci encoding human heavy and light chain immunoglobulins are inserted into the host's genome. The human genes are incorporated, for example, using yeast artificial chromosomes containing the requisite human DNA segments. An animal which provides all the desired modifications is then obtained as progeny by crossbreeding intermediate transgenic animals containing fewer than the full complement of the modifications. The preferred embodiment of such a nonhuman animal is a mouse, and is termed the Xenomouse™ as disclosed in PCT publications WO 96/33735 and WO 96/34096. This animal produces B cells which secrete fully human immunoglobulins. The antibodies can be obtained directly from the animal after immunization with an immunogen of interest, as, for example, a preparation of a polyclonal antibody, or alternatively from immortalized B cells derived from the animal, such as hybridomas producing monoclonal antibodies. Additionally, the genes encoding the immunoglobulins with human variable regions can be recovered and expressed to obtain the antibodies directly, or can be further modified to obtain analogs of antibodies such as, for example, single chain Fv molecules.


An example of a method of producing a nonhuman host, exemplified as a mouse, lacking expression of an endogenous immunoglobulin heavy chain is disclosed in U.S. Pat. No. 5,939,598. It can be obtained by a method including deleting the J segment genes from at least one endogenous heavy chain locus in an embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin heavy chain locus, the deletion being effected by a targeting vector containing a gene encoding a selectable marker; and producing from the embryonic stem cell a transgenic mouse whose somatic and germ cells contain the gene encoding the selectable marker.


A method for producing an antibody of interest, such as a human antibody, is disclosed in U.S. Pat. No. 5,916,771. It includes introducing an expression vector that contains a nucleotide sequence encoding a heavy chain into one mammalian host cell in culture, introducing an expression vector containing a nucleotide sequence encoding a light chain into another mammalian host cell, and fusing the two cells to form a hybrid cell. The hybrid cell expresses an antibody containing the heavy chain and the light chain.


In a further improvement on this procedure, a method for identifying a clinically relevant epitope on an immunogen, and a correlative method for selecting an antibody that binds immunospecifically to the relevant epitope with high affinity, are disclosed in PCT publication WO 99/53049.


Fab Fragments and Single Chain Antibodies


According to the invention, techniques can be adapted for the production of single-chain antibodies specific to an antigenic protein of the invention (see e.g., U.S. Pat. No. 4,946,778). In addition, methods can be adapted for the construction of Fab expression libraries (see e.g., Huse, et al., 1989 Science 246: 1275-1281) to allow rapid and effective identification of monoclonal Fab fragments with the desired specificity for a protein or derivatives, fragments, analogs or homologs thereof. Antibody fragments that contain the idiotypes to a protein antigen may be produced by techniques known in the art including, but not limited to: (i) an F(ab′)2 fragment produced by pepsin digestion of an antibody molecule; (ii) an Fab fragment generated by reducing the disulfide bridges of an F(ab′)2 fragment; (iii) an Fab fragment generated by the treatment of the antibody molecule with papain and a reducing agent and (iv) Fv fragments.


Bispecific Antibodies


Bispecific antibodies are monoclonal, preferably human or humanized, antibodies that have binding specificities for at least two different antigens. In the present case, one of the binding specificities is for an antigenic protein of the invention. The second binding target is any other antigen, and advantageously is a cell-surface protein or receptor or receptor subunit.


Methods for making bispecific antibodies are known in the art. Traditionally, the recombinant production of bispecific antibodies is based on the co-expression of two immunoglobulin heavy-chain/light-chain pairs, where the two heavy chains have different specificities (Mlstein and Cuello, Nature, 305:537-539 (1983)). Because of the random assortment of immunoglobulin heavy and light chains, these hybridomas (quadromas) produce a potential mixture of ten different antibody molecules, of which only one has the correct bispecific structure. The purification of the correct molecule is usually accomplished by affinity chromatography steps. Similar procedures are disclosed in WO 93/08829, published 13 May 1993, and in Traunecker et al., EMBO J., 10:3655-3659 (1991).


Antibody variable domains with the desired binding specificities (antibody-antigen combining sites) can be fused to immunoglobulin constant domain sequences. The fusion preferably is with an immunoglobulin heavy-chain constant domain, comprising at least part of the hinge, CH2, and CH3 regions. It is preferred to have the first heavy-chain constant region (CH1) containing the site necessary for light-chain binding present in at least one of the fusions. DNAs encoding the immunoglobulin heavy-chain fusions and, if desired, the immunoglobulin light chain, are inserted into separate expression vectors, and are co-transfected into a suitable host organism. For further details of generating bispecific antibodies see, for example, Suresh et al., Methods in Enzymology, 121:210 (1986).


According to another approach described in WO 96/27011, the interface between a pair of antibody molecules can be engineered to maximize the percentage of heterodimers which are recovered from recombinant cell culture. The preferred interface comprises at least a part of the CH3 region of an antibody constant domain. In this method, one or more small amino acid side chains from the interface of the first antibody molecule are replaced with larger side chains (e.g. tyrosine or tryptophan). Compensatory “cavities” of identical or similar size to the large side chain(s) are created on the interface of the second antibody molecule by replacing large amino acid side chains with smaller ones (e.g. alanine or threonine). This provides a mechanism for increasing the yield of the heterodimer over other unwanted end-products such as homodimers.


Bispecific antibodies can be prepared as full length antibodies or antibody fragments (e.g. F(ab′)2 bispecific antibodies). Techniques for generating bispecific antibodies from antibody fragments have been described in the literature. For example, bispecific antibodies can be prepared using chemical linkage. Brennan et al., Science 229:81 (1985) describe a procedure wherein intact antibodies are proteolytically cleaved to generate F(ab′)2 fragments. These fragments are reduced in the presence of the dithiol complexing agent sodium arsenite to stabilize vicinal dithiols and prevent intermolecular disulfide formation. The Fab′ fragments generated are then converted to thionitrobenzoate (TNB) derivatives. One of the Fab′-TNB derivatives is then reconverted to the Fab′-thiol by reduction with mercaptoethylamnine and is mixed with an equimolar amount of the other Fab′-TNB derivative to form the bispecific antibody. The bispecific antibodies produced can be used as agents for the selective immobilization of enzymes.


Additionally, Fab′ fragments can be directly recovered from E. coli and chemically coupled to form bispecific antibodies. Shalaby et al., J. Exp. Med. 175:217-225 (1992) describe the production of a fully humanized bispecific antibody F(ab′)2 molecule. Each Fab′ fragment was separately secreted from E. coli and subjected to directed chemical coupling in vitro to form the bispecific antibody. The bispecific antibody thus formed was able to bind to cells overexpressing the ErbB2 receptor and normal human T cells, as well as trigger the lytic activity of human cytotoxic lymphocytes against human breast tumor targets.


Various techniques for making and isolating bispecific antibody fragments directly from recombinant cell culture have also been described. For example, bispecific antibodies have been produced using leucine zippers. Kostelny et al., J. Immunol. 148(5):1547-1553 (1992). The leucine zipper peptides from the Fos and Jun proteins were linked to the Fab′ portions of two different antibodies by gene fusion. The antibody homodimers were reduced at the hinge region to form monomers and then re-oxidized to form the antibody heterodimers. This method can also be utilized for the production of antibody homodimers. The “diabody” technology described by Hollinger et al., Proc. Natl. Acad. Sci. USA 90:6444-6448 (1993) has provided an alternative mechanism for making bispecific antibody fragments. The fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) by a linker which is too short to allow pairing between the two domains on the same chain. Accordingly, the VH and VL domains of one fragment are forced to pair with the complementary VL and VH domains of another fragment, thereby forming two antigen-binding sites. Another strategy for making bispecific antibody fragments by the use of single-chain Fv (sFv) dimers has also been reported. See, Gruber et al., J. Imunol. 152:5368 (1994).


Antibodies with more than two valencies are contemplated. For example, trispecific antibodies can be prepared. Tutt et al., J. Immunol. 147:60 (1991).


Exemplary bispecific antibodies can bind to two different epitopes, at least one of which originates in the protein antigen of the invention. Alternatively, an anti-antigenic arm of an immunoglobulinr molecule can be combined with an arm which binds to a triggering molecule on a leukocyte such as a T-cell receptor molecule (e.g. CD2, CD3, CD28, or B7), or Fc receptors for IgG (FcγR), such as FcγRI (CD64), FcγRII (CD32) and FcγRIII (CD16) so as to focus cellular defense mechanisms to the cell expressing the particular antigen. Bispecific antibodies can also be used to direct cytotoxic agents to cells which express a particular antigen. These antibodies possess an antigen-binding arm and an arm which binds a cytotoxic agent or a radionuclide chelator, such as EOTUBE, DPTA, DOTA, or TETA. Another bispecific antibody of interest binds the protein antigen described herein and further binds tissue factor (TF).


Heteroconjugate Antibodies


Heteroconjugate antibodies are also within the scope of the present invention. Heteroconjugate antibodies are composed of two covalently joined antibodies. Such antibodies have, for example, been proposed to target immune system cells to unwanted cells (U.S. Pat. No. 4,676,980), and for treatment of HIV infection (WO 91/00360; WO 92/200373; EP 03089). It is contemplated that the antibodies can be prepared in vitro using known methods in synthetic protein chemistry, including those involving crosslinking agents. For example, immunotoxins can be constructed using a disulfide exchange reaction or by forming a thioether bond. Examples of suitable reagents for this purpose include iminothiolate and methyl-4-mercaptobutyrimidate and those disclosed, for example, in U.S. Pat. No. 4,676,980.


Effector Function Engineering


It can be desirable to modify the antibody of the invention with respect to effector function, so as to enhance, e.g., the effectiveness of the antibody in treating cancer. For example, cysteine residue(s) can be introduced into the Fc region, thereby allowing interchain disulfide bond formation in this region. The homodimeric antibody thus generated can have improved internalization capability and/or increased complement-mediated cell killing and antibody-dependent cellular cytotoxicity (ADCC). See Caron et al., J. Exp Med., 176: 1191-1195 (1992) and Shopes, J. Immunol., 148: 2918-2922 (1992). Homodimeric antibodies with enhanced anti-tumor activity can also be prepared using heterobifunctional cross-linkers as described in Wolff et al. Cancer Research, 53: 2560-2565 (1993). Alternatively, an antibody can be engineered that has dual Fc regions and can thereby have enhanced complement lysis and ADCC capabilities. See Stevenson et al., Anti-Cancer Drug Design, 3: 219-230 (1989).


Immunoconjugates


The invention also pertains to immunoconjugates comprising an antibody conjugated to a cytotoxic agent such as a chemotherapeutic agent, toxin (e.g., an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or fragments thereof), or a radioactive isotope (i.e., a radioconjugate).


Chemotherapeutic agents useful in the generation of such immunoconjugates have been described above. Enzymatically active toxins and fragments thereof that can be used include diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin, Aleurites fordii proteins, dianthin proteins, Phytolaca americana proteins (PAPI, PAPII, and PAP-S), momordica charantia inhibitor, curcin, crotin, sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the tricothecenes. A variety of radionuclides are available for the production of radioconjugated antibodies. Examples include 212Bi, 131I, 131In, 90Y, and 186Re.


Conjugates of the antibody and cytotoxic agent are made using a variety of bifunctional protein-coupling agents such as N-succinimidyl-3-(2-pyridyldithiol) propionate (SPDP), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HCL), active esters (such as disuccinimidyl suberate), aldehydes (such as glutareldehyde), bis-azido compounds (such as bis (p-azidobenzoyl) hexanediamine), bis-diazonium derivatives (such as bis-(p-diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as tolyene 2,6-diisocyanate), and bis-active fluorine compounds (such as 1,5-difluoro-2,4-dinitrobenzene). For example, a ricin immunotoxin can be prepared as described in Vitetta et al., Science, 238: 1098 (1987). Carbon-14-labeled 1-isothiocyanatobenzyl-3-methyldiethylene triaminepentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugation of radionucleotide to the antibody. See WO94/11026.


In another embodiment, the antibody can be conjugated to a “receptor” (such streptavidin) for utilization in tumor pretargeting wherein the antibody-receptor conjugate is administered to the patient, followed by removal of unbound conjugate from the circulation using a clearing agent and then administration of a “ligand” (e.g., avidin) that is in turn conjugated to a cytotoxic agent.


Immunoliposomes


The antibodies disclosed herein can also be formulated as immunoliposomes. Liposomes containing the antibody are prepared by methods known in the art, such as described in Epstein et al., Proc. Natl. Acad. Sci. USA, 82: 3688 (1985); Hwang et al., Proc. Natl Acad. Sci. USA, 77: 4030 (1980); and U.S. Pat. Nos. 4,485,045 and 4,544,545. Liposomes with enhanced circulation time are disclosed in U.S. Pat. No. 5,013,556.


Particularly useful liposomes can be generated by the reverse-phase evaporation method with a lipid composition comprising phosphatidylcholine, cholesterol, and PEG-derivatized phosphatidylethanolamine (PEG-PE). Liposomes are extruded through filters of defined pore size to yield liposomes with the desired diameter. Fab′ fragments of the antibody of the present invention can be conjugated to the liposomes as described in Martin et al., J. Biol. Chem., 257: 286-288 (1982) via a disulfide-interchange reaction. A chemotherapeutic agent (such as Doxorubicin) is optionally contained within the liposome. See Gabizon et al., J. National Cancer Inst., 81(19): 1484 (1989).


Diagnostic Applications of Antibodies Directed Against the Proteins of the Invention


In one embodiment, methods for the screening of antibodies that possess the desired specificity include, but are not limited to, enzyme linked immunosorbent assay (ELISA) and other immunologically mediated techniques known within the art. In a specific embodiment, selection of antibodies that are specific to a particular domain of an NOVX protein is facilitated by generation of hybridomas that bind to the fragment of an NOVX protein possessing such a domain. Thus, antibodies that are specific for a desired domain within an NOVX protein, or derivatives, fragments, analogs or homologs thereof, are also provided herein.


Antibodies directed against a NOVX protein of the invention may be used in methods known within the art relating to the localization and/or quantitation of a NOVX protein (e.g., for use in measuring levels of the NOVX protein within appropriate physiological samples, for use in diagnostic methods, for use in imaging the protein, and the like). In a given embodiment, antibodies specific to a NOVX protein, or derivative, fragment, analog or homolog thereof, that contain the antibody derived antigen binding domain, are utilized as pharmacologically active compounds (referred to hereinafter as “Therapeutics”).


An antibody specific for a NOVX protein of the invention (e.g., a monoclonal antibody or a polyclonal antibody) can be used to isolate a NOVX polypeptide by standard techniques, such as immunoaffinity, chromatography or immunoprecipitation. An antibody to a NOVX polypeptide can facilitate the purification of a natural NOVX antigen from cells, or of a recombinantly produced NOVX antigen expressed in host cells. Moreover, such an anti-NOVX antibody can be used to detect the antigenic NOVX protein (e.g., in a cellular lysate or cell supernatant) in order to evaluate the abundance and pattern of expression of the antigenic NOVX protein. Antibodies directed against a NOVX protein can be used diagnostically to monitor protein levels in tissue as part of a clinical testing procedure, e.g., to, for example, determine the efficacy of a given treatment regimen. Detection can be facilitated by coupling (ie., physically linking) the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, β-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include 125I, 131I, 35S or 3H.


Antibody Therapeutics


Antibodies of the invention, including polyclonal, monoclonal, humanized and fully human antibodies, may used as therapeutic agents. Such agents will generally be employed to treat or prevent a disease or pathology in a subject. An antibody preparation, preferably one having high specificity and high affinity for its target antigen, is administered to the subject and will generally have an effect due to its binding with the target. Such an effect may be one of two kinds, depending on the specific nature of the interaction between the given antibody molecule and the target antigen in question. In the first instance, administration of the antibody may abrogate or inhibit the binding of the target with an endogenous ligand to which it naturally binds. In this case, the antibody binds to the target and masks a binding site of the naturally occurring ligand, wherein the ligand serves as an effector molecule. Thus the receptor mediates a signal transduction pathway for which ligand is responsible.


Alternatively, the effect may be one in which the antibody elicits a physiological result by virtue of binding to an effector binding site on the target molecule. In this case the target, a receptor having an endogenous ligand which may be absent or defective in the disease or pathology, binds the antibody as a surrogate effector ligand, initiating a receptor-based signal transduction event by the receptor.


A therapeutically effective amount of an antibody of the invention relates generally to the amount needed to achieve a therapeutic objective. As noted above, this may be a binding interaction between the antibody and its target antigen that, in certain cases, interferes with the functioning of the target, and in other cases, promotes a physiological response. The amount required to be administered will furthermore depend on the binding affinity of the antibody for its specific antigen, and will also depend on the rate at which an administered antibody is depleted from the free volume other subject to which it is administered. Common ranges for therapeutically effective dosing of an antibody or antibody fragment of the invention may be, by way of nonlimiting example, from about 0.1 mg/kg body weight to about 50 mg/kg body weight. Common dosing frequencies may range, for example, from twice daily to once a week.


Pharmaceutical Compositions of Antibodies


Antibodies specifically binding a protein of the invention, as well as other molecules identified by the screening assays disclosed herein, can be administered for the treatment of various disorders in the form of pharmaceutical cormpositions. Principles and considerations involved in preparing such compositions, as well as guidance in the choice of components are provided, for example, in Remington: The Science And Practice Of Pharmacy 19th ed. (Alfonso R. Gennaro, et al., editors) Mack Pub. Co., Easton, Pa.: 1995; Drug Absorption Enhancement: Concepts, Possibilities, Limitations, And Trends, Harwood Academic Publishers, Langhorne, Pa., 1994; and Peptide And Protein Drug Delivery (Advances In Parenteral Sciences, Vol. 4), 1991, M. Dekker, New York.


If the antigenic protein is intracellular and whole antibodies are used as inhibitors, internalizing antibodies are preferred. However, liposomes can also be used to deliver the antibody, or an antibody fragment, into cells. Where antibody fragments are used, the smallest inhibitory fragment that specifically binds to the binding domain of the target protein is preferred. For example, based upon the variable-region sequences of an antibody, peptide molecules can be designed that retain the ability to bind the target protein sequence. Such peptides can be synthesized chemically and/or produced by recombinant DNA technology. See, e.g., Marasco et al., Proc. Natl. Acad. Sci. USA, 90: 7889-7893 (1993). The formulation herein can also contain more than one active compound as necessary for the particular indication being treated, preferably those with complementary activities that do not adversely affect each other. Alternatively, or in addition, the composition can comprise an agent that enhances its function, such as, for example, a cytotoxic agent, cytokine, chemotherapeutic agent, or growth-inhibitory agent. Such molecules are suitably present in combination in amounts that are effective for the purpose intended.


The active ingredients can also be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacrylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles, and nanocapsules) or in macroemulsions.


The formulations to be used for in vivo administration must be sterile. This is readily accomplished by filtration through sterile filtration membranes.


Sustained-release preparations can be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the antibody, which matrices are in the form of shaped articles, e.g., films, or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (for example, poly(2-hydroxyethyl-methacrylate), or poly(vinylalcohol)), polylactides (U.S. Pat. No. 3,773,919), copolymers of L-glutamic acid and γ ethyl-L-glutamate, non-degradable ethylene-vinyl acetate, degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOT™ (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(−)-3-hydroxybutyric acid. While polymers such as ethylene-vinyl acetate and lactic acid-glycolic acid enable release of molecules for over 100 days, certain hydrogels release proteins for shorter time periods.


ELISA Assay


An agent for detecting an analyte protein is an antibody capable of binding to an analyte protein, preferably an antibody with a detectable label. Antibodies can be polyclonal, or more preferably, monoclonal. An intact antibody, or a fragment thereof (e.g., Fab or F(ab)2) can be used. The term “labeled”, with regard to the probe or antibody, is intended to encompass direct labeling of the probe or antibody by coupling (i.e., physically linking) a detectable substance to the probe or antibody, as well as indirect labeling of the probe or antibody by reactivity with another reagent that is directly labeled. Examples of indirect labeling include detection of a primary antibody using a fluorescently-labeled secondary antibody and end-labeling of a DNA probe with biotin such that it can be detected with fluorescently-labeled streptavidin. The term “biological sample” is intended to include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject. Included within the usage of the term “biological sample”, therefore, is blood and a fraction or component of blood including blood serum, blood plasma, or lymph. That is, the detection method of the invention can be used to detect an analyte mRNA, protein, or genomic DNA in a biological sample in vitro as well as in vivo. For example, in vitro techniques for detection of an analyte mRNA include Northern hybridizations and in situ hybridizations. In vitro techniques for detection of an analyte protein include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations, and immunofluorescence. In vitro techniques for detection of an analyte genomic DNA include Southern hybridizations. Procedures for conducting immunoassays are described, for example in “ELISA: Theory and Practice: Methods in Molecular Biology”, Vol. 42, J. R. Crowther (Ed.) Human Press, Totowa, N.J., 1995; “Immunoassay”, E. Diamandis and T. Christopoulus, Academic Press, Inc., San Diego, Calif., 1996; and “Practice and Theory of Enzyme Immunoassays”, P. Tijssen, Elsevier Science Publishers, Amsterdam, 1985. Furthermore, in vivo techniques for detection of an analyte protein include introducing into a subject a labeled anti-an analyte protein antibody. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques.


NOVX Recombinant Expression Vectors and Host Cells


Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding a NOVX protein, or derivatives, fragments, analogs or homologs thereof. As used herein, the term “vector” refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid”, which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively-linked. Such vectors are referred to herein as “expression vectors”. In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.


The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, that is operatively-linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, “operably-linked” is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner that allows for expression of the nucleotide sequence (e.g.; in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell).


The term “regulatory sequence” is intended to includes promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cell and those that direct expression of the nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences). It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein (e.g., NOVX proteins, mutant forms of NOVX proteins, fusion proteins, etc.).


The recombinant expression vectors of the invention can be designed for expression of NOVX proteins in prokaryotic or eukaryotic cells. For example, NOVX proteins can be expressed in bacterial cells such as Escherichia coli, insect cells (using baculovirus expression vectors) yeast cells or mammalian cells. Suitable host cells are discussed further in Goeddel, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and 17 polymerase.


Expression of proteins in prokaryotes is most often carried out in Escherichia coli with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein. Such fusion vectors typically serve three purposes: (i) to increase expression of recombinant protein; (ii) to increase the solubility of the recombinant protein; and (iii) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase. Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith and Johnson, 1988. Gene 67: 31-40), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) that fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein.


Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amrann et al., (1988) Gene 69:301-315) and pET 11d (Studier et al., GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990) 60-89).


One strategy to maximize recombinant protein expression in E. coli is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein. See, e.g., Gottesman, GENE EXPRESSION TECHNOLOGY: METHODS IN ENZYMOLOGY 185, Academic Press, San Diego, Calif. (1990) 119-128. Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in E. coli (see, e.g., Wada, et al., 1992. Nucl. Acids Res. 20: 2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.


In another embodiment, the NOVX expression vector is a yeast expression vector. Examples of vectors for expression in yeast Saccharomyces cerivisae include pYepSec1 (Baldari, et al., 1987. EMBO J. 6: 229-234), pMFa (Kurjan and Herskowitz, 1982. Cell 30: 933-943), pJRY88 (Schultz et al., 1987. Gene 54: 113-123), pYES2 (Invitrogen Corporation, San Diego, Calif.), and picZ (In Vitrogen Corp, San Diego, Calif.).


Alternatively, NOVX can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., SF9 cells) include the pAc series (Smith, et al., 1983. Mol. Cell. Biol. 3: 2156-2165) and the pVL series (Lucklow and Summers, 1989. Virology 170: 31-39).


In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, 1987. Nature 329: 840) and pMT2PC (Kaufman, et al., 1987. EMBO J. 6: 187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma, adenovirus 2, cytomegalovirus, and simian virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see, e.g., Chapters 16 and 17 of Sambrook, et al., MOLECULAR CLONING: A LABORATORY MANUAL. 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.


In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert, et al., 1987. Genes Dev. 1: 268-277), lymphoid-specific promoters (Calame and Eaton, 1988. Adv. Immunol. 43: 235-275), in particular promoters of T cell receptors (Winoto and Baltimore, 1989. EMBO J. 8: 729-733) and immunoglobulins (Banerji, et al., 1983. Cell 33: 729-740; Queen and Baltimore, 1983. Cell 33: 741-748), neuron-specific promoters (e.g., the neurofilament promoter; Byrne and Ruddle, 1989. Proc. Natl. Acad. Sci USA 86: 5473-5477), pancreas-specific promoters (Edlund, et al., 1985. Science 230: 912-916), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Pat. No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, e.g., the murine hox promoters (Kessel and Gruss, 1990. Science 249: 374-379) and the α-fetoprotein promoter (Campes and Tilghman, 1989. Genes Dev. 3: 537-546).


The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively-linked to a regulatory sequence in a manner that allows for expression (by transcription of the DNA molecule) of an RNA molecule that is antisense to NOVX mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen that direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen that direct constitutive, tissue specific or cell type specific expression of antisense RNA. The antisense expression vector can be in the form of a recombinant plasrid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see, e.g., Weintraub, et al., “Antisense RNA as a molecular tool for genetic analysis,” Reviews—Trends in Genetics, Vol. 1(1) 1986.


Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms “host cell” and “recombinant host cell” are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.


A host cell can be any prokaryotic or eukaryotic cell. For example, NOVX protein can be expressed in bacterial cells such as E. coli , insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those skilled in the art.


Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms “transformation” and “transfection” are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (MOLECULAR CLONING: A LABORATORY MANUAL. 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989), and other laboratory manuals.


For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker (e.g., resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Various selectable markers include those that confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding NOVX or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by drug selection (e.g., cells that have incorporated the selectable marker gene will survive, while the other cells die).


A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce (i.e., express) NOVX protein. Accordingly, the invention further provides methods for producing NOVX protein using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding NOVX protein has been introduced) in a suitable medium such that NOVX protein is produced. In another embodiment, the method further comprises isolating NOVX protein from the medium or the host cell.


Transgenic NOVX Animals


The host cells of the invention can also be used to produce non-human transgenic animals. For example, in one embodiment, a host cell of the invention is a fertilized oocyte or an embryonic stem cell into which NOVX protein-coding sequences have been introduced. Such host cells can then be used to create non-human transgenic animals in which exogenous NOVX sequences have been introduced into their genome or homologous recombinant animals in which endogenous NOVX sequences have been altered. Such animals are useful for studying the function and/or activity of NOVX protein and for identifying and/or evaluating modulators of NOVX protein activity. As used herein, a “transgenic animal” is a non-human animal, preferably a mammal, more preferably a rodent such as a rat or mouse, in which one or more of the cells of the animal. includes a transgene. Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, amphibians, etc. A transgene is exogenous DNA that is integrated into the genome of a cell from which a transgenic animal develops and that remains in the genome of the mature animal, thereby directing the expression of an encoded gene product in one or more cell types or tissues of the transgenic animal. As used herein, a “homologous recombinant animal” is a non-human animal, preferably a mammal, more preferably a mouse, in which an endogenous NOVX gene has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal, e.g., an embryonic cell of the animal, prior to development of the animal.


A transgenic animal of the invention can be created by introducing NOVX-encoding nucleic acid into the male pronuclei of a fertilized oocyte (e.g., by microinjection, retroviralinfection) and allowing the oocyte to develop in a pseudopregnant female foster animal. The human NOVX cDNA sequences, i.e., any one of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, can be introduced as a transgene into the genome of a non-human animal. Alternatively, a non-human homologue of the human NOVX gene, such as a mouse NOVX gene, can be isolated based on hybridization to the human NOVX cDNA (described further supra) and used as a transgene. Intronic sequences and polyadenylation signals can also be included in the transgene to increase the efficiency of expression of the transgene. A tissue-specific regulatory sequence(s) can be operably-linked to the NOVX transgene to direct expression of NOVX protein to particular cells. Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866; 4,870,009; and 4,873,191; and Hogan, 1986. In: MANIPULATING THE MOUSE EMBRYO, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Similar methods are used for production of other transgenic animals. A transgenic founder animal can be identified based upon the presence of the NOVX transgene in its genome and/or expression of NOVX mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a transgene-encoding NOVX protein can further be bred to other transgenic animals carrying other transgenes.


To create a homologous recombinant animal, a vector is prepared which contains at least a portion of a NOVX gene into which a deletion, addition or substitution has been introduced to thereby alter, e.g., functionally disrupt, the NOVX gene. The NOVX gene can be a human gene (e.g., the cDNA of any one of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606), but more preferably, is a non-human homologue of a human NOVX gene. For example, a mouse homologue of human NOVX gene of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, can be used to construct a homologous recombination vector suitable for altering an endogenous NOVX gene in the mouse genome. In one embodiment, the vector is designed such that, upon homologous recombination, the endogenous NOVX gene is functionally disrupted (i.e., no longer encodes a functional protein; also referred to as a “knock out” vector).


Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous NOVX gene is mutated or otherwise altered but still encodes functional protein (e.g., the upstream regulatory region can be altered to thereby alter the expression of the endogenous NOVX protein). In the homologous recombination vector, the altered portion of the NOVX gene is flanked at its 5′- and 3′-termini by additional nucleic acid of the NOVX gene to allow for homologous recombination to occur between the exogenous NOVX gene carried by the vector and an endogenous NOVX gene in an embryonic stem cell. The additional flanking NOVX nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5′- and 3′-termini) are included in the vector. See, e.g., Thomas, et al., 1987. Cell 51: 503 for a description of homologous recombination vectors. The vector is ten introduced into an embryonic stem cell line (e.g., by electroporation) and cells in which the introduced NOVX gene has homologously-recombined with the endogenous NOVX gene are selected. See, e.g., Li, et al., 1992. Cell 69: 915.


The selected cells are then injected into a blastocyst of an animal (e.g., a mouse) to form aggregation chimeras. See, e.g., Bradley, 1987. In: TERATOCARCINOMAS AND EMBRYONIC STEM CELLS: A PRACTICAL APPROACH, Robertson, ed. IRL, Oxford, pp. 113-152. A chimeric embryo can then be implanted into a suitable pseudopregnant female foster animal and the embryo brought to term. Progeny harboring the homologously-recombined DNA in their germ cells can be used to breed animals in which all cells of the animal contain the homologously-recombined DNA by germline transmission of the transgene. Methods for constructing homologous recombination vectors and homologous recombinant animals are described further in Bradley, 1991. Curr. Opin. Biotechnol. 2: 823-829; PCT International Publication Nos.: WO 90/11354; WO 91/01140; WO 92/0968; and WO 93/04169.


In another embodiment, transgenic non-humans animals can be produced that contain selected systems that allow for regulated expression of the transgene. One example of such a system is the cre/loxP recombinase system of bacteriophage P1. For a description of the cre/loxP recombinase system, See, e.g., Lakso, et al., 1992. Proc. Natl. Acad. Sci. USA 89: 6232-6236. Another example of a recombinase system is the FLP recombinase system of Saccharomyces cerevisiae. See, O'Gorman, et al., 1991. Science 251:1351-1355. If a cre/loxP recombinase system is used to regulate expression of the transgene, animals containing transgenes encoding both the Cre recombinase and a selected protein are required. Such animals can be provided through the construction of “double” transgenic animals, e.g., by mating two transgenic animals, one containing a transgene encoding a selected protein and the other containing a transgene encoding a recombinase.


Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut, et al., 1997. Nature 385: 810-813. In brief, a cell (e.g., a somatic cell) from the transgenic animal can be isolated and induced to exit the growth cycle and enter G0 phase. The quiescent cell can then be fused, e.g., through. the use of electrical pulses, to an enucleated oocyte from an animal of the same species from which the quiescent cell is isolated. The reconstructed oocyte is then cultured such that it develops to morula or blastocyte and then transferred to pseudopregnant female foster animal. The offspring borne of this female foster animal will be a clone of the animal from which the cell (e.g., the somatic cell) is isolated.


Pharmaceutical Compositions


The NOVX nucleic acid molecules, NOVX proteins, and anti-NOVX antibodies (also referred to herein as “active compounds”) of the invention, and derivatives, fragments, analogs and homologs thereof, can be incorporated into pharmaceutical compositions suitable for administration. Such compositions typically comprise the nucleic acid molecule, protein, or antibody and a pharmaceutically acceptable carrier. As used herein, “pharmaceutically acceptable carrier” is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Suitable carriers are described in the most recent edition of Remington's Pharmaceutical Sciences, a standard reference text in the field, which is incorporated herein by reference. Preferred examples of such carriers or diluents include, but are not limited to, water, saline, finger's solutions, dextrose solution, and 5% human serum albumin. Liposomes and non-aqueous vehicles such as fixed oils may also be used. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the compositions is contemplated. Supplementary active compounds can also be incorporated into the compositions.


A pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (i.e., topical), transmucosal, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid (EDTA); buffers such as acetates, citrates or phosphates, and agents for the adjustment of tonicity such as sodium chloride or dextrose. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.


Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, N.J.) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringeability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as manitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.


Sterile injectable solutions can be prepared by incorporating the active compound (e.g., a NOVX protein or anti-NOVX antibody) in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle that contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, methods of preparation are vacuum drying and freeze-drying that yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.


Oral compositions generally include an inert diluent or an edible carrier. They can be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash, wherein the compound in the fluid carrier is applied orally and swished and expectorated or swallowed. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring.


For administration by inhalation, the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.


Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.


The compounds can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.


In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Pat. No. 4,522,811.


It is especially advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of individuals.


The nucleic acid molecules of the invention can be inserted into vectors and used as gene therapy vectors. Gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration (see, e.g., U.S. Pat. No. 5,328,470) or by stereotactic injection (see, e.g., Chen, et al., 1994. Proc. Natl. Acad. Sci. USA 91: 3054-3057). The pharmaceutical preparation of the gene therapy vector can include the gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded. Alternatively, where the complete gene delivery vector can be produced intact from recombinant cells, e.g., retroviral vectors, the pharmaceutical preparation can include one or more cells that produce the gene delivery system.


The pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration.


Screening and Detection Methods


The isolated nucleic acid molecules of the invention can be used to express NOVX protein (e.g., via a recombinant expression vector in a host cell in gene therapy applications), to detect NOVX mRNA (e.g., in a biological sample) or a genetic lesion in a NOVX gene, and to modulate NOVX activity, as described further, below. In addition, the NOVX proteins can be used to screen drugs or compounds that modulate the NOVX protein activity or expression as well as to treat disorders characterized by insufficient or excessive production of NOVX protein or production of NOVX protein forms that have decreased or aberrant activity compared to NOVX wild-type protein (e.g.; diabetes (regulates insulin release); obesity (binds and transport lipids); metabolic disturbances associated with obesity, the metabolic syndrome X as well as anorexia and wasting disorders associated with chronic diseases and various cancers, and infectious disease(possesses anti-microbial activity) and the various dyslipidemias. In addition, the anti-NOVX antibodies of the invention can be used to detect and isolate NOVX proteins and modulate NOVX activity. In yet a further aspect, the invention can be used in methods to influence appetite, absorption of nutrients and the disposition of metabolic substrates in both a positive and negative fashion.


The invention further pertains to novel agents identified by the screening assays described herein and uses thereof for treatments as described, supra.


Screening Assays


The invention provides a method (also referred to herein as a “screening assay”) for identifying modulators, i.e., candidate or test compounds or agents (e.g., peptides, peptidomimetics, small molecules or other drugs) that bind to NOVX proteins or have a stimulatory or inhibitory effect on, e.g., NOVX protein expression or NOVX protein activity. The invention also includes compounds identified in the screening assays described herein.


In one embodiment, the invention provides assays for screening candidate or test compounds which bind to or modulate the activity of the membrane-bound form of a NOVX protein or polypeptide or biologically-active portion thereof. The test compounds of the invention can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; the “one-bead one-compound” library method; and synthetic library methods using affinity chromatography selection. The biological library approach is limited to peptide libraries, while the other four approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds. See, e.g., Lam, 1997. Anticancer Drug Design 12: 145.


A “small molecule” as used herein, is meant to refer to a composition that has a molecular weight of less than about 5 kD and most preferably less than about 4 kD. Small molecules can be, e.g., nucleic acids, peptides, polypeptides, peptidomimetics, carbohydrates, lipids or other organic or inorganic molecules. Libraries of chemical and/or biological mixtures, such as fungal, bacterial, or algal extracts, are klown in the art and can be screened with any of the assays of the invention.


Examples of methods for the synthesis of molecular libraries can be found in the art, for example in: DeWitt, et al., 1993. Proc. Natl. Acad. Sci. U.S.A. 90: 6909; Erb, et al., 1994. Proc. Natl. Acad. Sci. U.S.A. 91: 11422; Zuckermann, et al., 1994. J. Med. Chem. 37: 2678; Cho, et al., 1993. Science 261: 1303; Carrell, et al., 1994. Angew. Chem. Int. Ed. Engl. 33: 2059; Carell, et al., 1994. Angew. Chem. Int. Ed. Engl. 33: 2061; and Gallop, et al., 1994. J. Med. Chem. 37: 1233.


Libraries of compounds may be presented in solution (e.g., Houghten, 1992. Biotechniques 13: 412421), or on beads (Lam, 1991. Nature 354: 82-84), on chips (Fodor, 1993. Nature 364: 555-556), bacteria (Ladner, U.S. Pat. No. 5,223,409), spores (Ladner, U.S. Pat. No. 5,233,409), plasmids (Cull, et al., 1992. Proc. Natl. Acad. Sci. USA 89: 1865-1869) or on phage (Scott and Smith, 1990. Science 249: 386-390; Devlin, 1990. Science 249: 404-406; Cwirla, et al., 1990. Proc. Natl. Acad. Sci. U.S.A. 87: 6378-6382; Felici, 1991. J. Mol. Biol. 222: 301-310; Ladner, U.S. Pat. No. 5,233,409.).


In one embodiment, an assay is a cell-based assay in which a cell which expresses a membrane-bound form of NOVX protein, or atbiologically-active portion thereof, on the cell surface is contacted with a test compound and the ability of the test compound to bind to a NOVX protein determined. The cell, for example, can of mammalian origin or a yeast cell. Determining the ability of the test compound to bind to the NOVX protein can be accomplished, for example, by coupling the test compound with a radioisotope or enzymatic label such that binding of the test compound to the NOVX protein or biologically-active portion thereof can be determined by detecting the labeled compound in a complex. For example, test compounds can be labeled with 1251, 35S, 14C, or 3H, either directly or indirectly, and the radioisotope detected by direct counting of radioemission or by scintillation counting. Alternatively, test compounds can be enzymatically-labeled with, for example, horseradish peroxidase, alkaline phosphatase, or luciferase, and the enzymatic label detected by determination of conversion of an appropriate substrate to product. In one embodiment, the assay comprises contacting a cell which expresses a membrane-bound form of NOVX protein, or a biologically-active portion thereof, on the cell surface with a known compound which binds NOVX to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a NOVX protein, wherein determining the ability of the test compound to interact with a NOVX protein comprises determining the ability of the test compound to preferentially bind to NOVX protein or a biologically-active portion thereof as compared to the known compound.


In another embodiment, an assay is a cell-based assay comprising contacting a cell expressing a membrane-bound form of NOVX protein, or a biologically-active portion thereof, on the cell surface with a test compound and determining the ability of the test compound to modulate (e.g., stimulate or inhibit) the activity of the NOVX protein or biologically-active portion thereof. Determining the ability of the test compound to modulate the activity of NOVX or a biologically-active portion thereof can be accomplished, for example, by determining the ability of the NOVX protein to bind to or interact with a NOVX target molecule. As used herein, a “target molecule” is a molecule with which a NOVX protein binds or interacts in nature, for example, a molecule on the surface of a cell which expresses a NOVX interacting protein, a molecule on the surface of a second cell, a molecule in the extracellular milieu, a molecule associated with the internal surface of a cell membrane or a cytoplasmic molecule. A NOVX target molecule can be a non-NOVX molecule or a NOVX protein or polypeptide of the invention. In one embodiment, a NOVX target molecule is a component of a signal transduction pathway that facilitates transduction of an extracellular signal (e.g. a signal generated by binding of a compound to a membrane-bound NOVX molecule) through the cell membrane and into the cell. The target, for example, can be a second intercellular protein that has catalytic activity or a protein that facilitates the association of downstream signaling molecules with NOVX.


Determining the ability of the NOVX protein to bind to or interact with a NOVX target molecule can be accomplished by one of the methods described above for determining direct binding. In one embodiment, determining the ability of the NOVX protein to bind to or interact with a NOVX target molecule can be accomplished by determining the activity of the target molecule. For example, the activity of the target molecule can be determined by detecting induction of a cellular second messenger of the target (i.e. intracellular Ca2+, diacylglycerol, IP3, etc.), detecting catalytic/enzymatic activity of the target an appropriate substrate, detecting the induction of a reporter gene (comprising a NOVX-responsive regulatory element operatively linked to a nucleic acid encoding a detectable marker, e.g., luciferase), or detecting a cellular response, for example, cell survival, cellular differentiation, or cell proliferation.


In yet another embodiment, an assay of the invention is a cell-free assay comprising contacting a NOVX protein or biologically-active portion thereof with a test compound and determining the ability of the test compound to bind to the NOVX protein or biologically-active portion thereof. Binding of the test compound to the NOVX protein can be determined either directly or indirectly as described above. In one such embodiment, the assay comprises contacting the NOVX protein or biologically-active portion thereof with a known compound which binds NOVX to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a NOVX protein, wherein determining the ability of the test compound to interact with a NOVX protein comprises determining the ability of the test compound to preferentially bind to NOVX or biologically-active portion thereof as compared to the known compound.


In still another embodiment, an assay is a cell-free assay comprising contacting NOVX protein or biologically-active portion thereof with a test compound and determining the ability of the test compound to modulate (e.g. stimulate or inhibit) the activity of the NOVX protein or biologically-active portion thereof. Determining the ability of the test compound to modulate the activity of NOVX can be accomplished, for example, by determining the ability of the NOVX protein to bind to a NOVX target molecule by one of the methods described above for determining direct binding. In an alternative embodiment, determining the ability of the test compound to modulate the activity of NOVX protein can be accomplished by determining the ability of the NOVX protein further modulate a NOVX target molecule. For example, the catalytic/enzymatic activity of the target molecule on an appropriate substrate can be determined as described, supra.


In yet another embodiment, the cell-free assay comprises contacting the NOVX protein or biologically-active portion thereof with a known compound which binds NOVX protein to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with a NOVX protein, wherein determining the ability of the test compound to interact with a NOVX protein comprises determining the ability of the NOVX protein to preferentially bind to or modulate the activity of a NOVX target molecule.


The cell-free assays of the invention are amenable to use of both the soluble form or the membrane-bound form of NOVX protein. In the case of cell-free assays comprising the membrane-bound form of NOVX protein, it may be desirable to utilize a solubilizing agent such that the membrane-bound form of NOVX protein is maintained in solution. Examples of such solubilizing agents include non-ionic detergents such as n-octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl-N-methylglucaamide, decanoyl-N-methylglucarnide, Triton® X-100, Triton® X-114, Thesit®, Isotridecypoly(ethylene glycol ether)n, N-dodecyl--N,N-dimethyl-3-ammonio-1-propane sulfonate, 3-(3-cholamidopropyl) dimethylamminiol-1-propane sulfonate (CHAPS), or 3-(3-cholamidopropyl)dimethylarmniniol-2-hydroxy-1-propane sulfonate (CHAPSO).


In more than one embodiment of the above assay methods of the invention, it may be desirable to immobilize either NOVX protein or its target molecule to facilitate separation of complexed from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay. Binding of a test compound to NOVX protein, or interaction of NOVX protein with a target molecule in the presence and absence of a candidate compound, can be accomplished in any vessel suitable for containing the reactants. Examples of such vessels include microtiter plates, test tubes, and micro-centrifuge tubes. In one embodiment, a fusion protein can be provided that adds a domain that allows one or both of the proteins to be bound to a matrix. For example, GST-NOVX fusion proteins or GST-target fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) or glutathione derivatized microtiter plates, that are then combined with the test compound or the test compound and either the non-adsorbed target protein or NOVX protein, and the mixture is incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtiter plate wells are washed to remove any unbound components, the matrix immobilized in the case of beads, complex determined either directly or indirectly, for example, as described, supra. Alternatively, the complexes can be dissociated from the matrix, and the level of NOVX protein binding or activity determined using standard techniques.


Other techniques for immobilizing proteins on matrices can also be used in the screening assays of the invention. For example, either the NOVX protein or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin. Biotinylated NOVX protein or target molecules can be prepared from biotin-NHS (N-hydroxy-succinimide) using techniques well-known within the art (e.g., biotinylation kit, Pierce Chemicals, Rockford, Ill.), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical). Alternatively, antibodies reactive with NOVX protein or target molecules, but which do not interfere with binding of the NOVX protein to its target molecule, can be derivatized to the wells of the plate, and unbound target or NOVX protein trapped in the wells by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the NOVX protein or target molecule, as well as enzyme-linked assays that rely on detecting an enzymatic activity associated with the NOVX protein or target molecule.


In another embodiment, modulators of NOVX protein expression are identified in a method wherein a cell is contacted with a candidate compound and the expression of NOVX mRNA or protein in the cell is determined. The level of expression of NOVX mRNA or protein in the presence of the candidate compound is compared to the level of expression of NOVX mRNA or protein in the absence of the candidate compound. The candidate compound can then be identified as a modulator of NOVX mRNA or protein expression based upon this comparison. For example, when expression of NOVX mRNA or protein is greater (i.e., statistically significantly greater) in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of NOVX mRNA or protein expression. Alternatively, when expression of NOVX rnRNA or protein is less (statistically significantly less) in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of NOVX mRNA or protein expression. The level of NOVX mRNA or protein expression in the cells can be determined by methods described herein for detecting NOVX mRNA or protein.


In yet another aspect of the invention, the NOVX proteins can be used as “bait proteins” in a two-hybrid assay or three hybrid assay (see, e.g., U.S. Pat. No. 5,283,317; Zervos, et al., 1993. Cell 72: 223-232; Madura, et al., 1993. J. Biol. Chem. 268: 12046-12054; Bartel, et al., 1993. Biotechniques 14: 920-924; Iwabuchi, et al., 1993. Oncogene 8: 1693-1696; and Brent WO 94/10300), to identify other proteins that bind to or interact with NOVX (“NOVX-binding proteins” or “NOVX-bp”) and modulate NOVX activity. Such NOVX-binding proteins are also involved in the propagation of signals by the NOVX proteins as, for example, upstream or downstream elements of the NOVX pathway.


The two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. Briefly, the assay utilizes two different DNA constructs. In one construct, the gene that codes for NOVX is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In the other construct, a DNA sequence, from a library of DNA sequences, that encodes an unidentified protein (“prey” or “sample”) is fused to a gene that codes for the activation domain of the known transcription factor. If the “bait” and the “prey” proteins are able to interact, in vivo, forming a NOVX-dependent complex, the DNA-binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) that is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to obtain the cloned gene that encodes the protein which interacts with NOVX.


The invention further pertains to novel agents identified by the aforementioned screening assays and uses thereof for treatments as described herein.


Detection Assays


Portions or fragments of the cDNA sequences identified herein (and the corresponding complete gene sequences) can be used in numerous ways as polynucleotide reagents. By way of example, and not of limitation, these sequences can be used to: (i) map their respective genes on a chromosome; and, thus, locate gene regions associated with genetic disease; (ii) identify an individual from a minute biological sample (tissue typing); and (iii) aid in forensic identification of a biological sample. Some of these applications are described in the subsections, below.


Chromosome Mapping


Once the sequence (or a portion of the sequence) of a gene has been isolated, this sequence can be used to map the location of the gene on a chromosome. This process is called chromosome mapping. Accordingly, portions or fragments of the NOVX sequences of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or fragments or derivatives thereof, can be used to map the location of the NOVX genes, respectively, on a chromosome. The mapping of the NOVX sequences to chromosomes is an important first step in correlating these sequences with genes associated with disease.


Briefly, NOVX genes can be mapped to chromosomes by preparing PCR primers (preferably 15-25 bp in length) from the NOVX sequences. Computer analysis of the NOVX, sequences can be used to rapidly select primers that do not span more than one exon in the genomic DNA, thus complicating the amplification process. These primers can then be used for PCR screening of somatic cell hybrids containing individual human chromosomes. Only those hybrids containing the human gene corresponding to the NOVX sequences will yield an amplified fragment.


Somatic cell hybrids are prepared by fusing somatic cells from different mammals (e.g., human and mouse cells). As hybrids of human and mouse cells grow and divide, they gradually lose human chromosomes in random order, but retain the mouse chromosomes. By using media in which mouse cells cannot grow, because they lack a particular enzyme, but in which human cells can, the one human chromosome that contains the gene encoding the needed enzyme will be retained. By using various media, panels of hybrid cell lines can be established. Each cell line in a panel contains either a single human chromosome or a small number of human chromosomes, and a full set of mouse chromosomes, allowing easy mapping of individual genes to specific human chromosomes. See, e.g., D'Eustachio, et al., 1983. Science 220: 919-924. Somatic cell hybrids containing only fragments of human chromosomes can also be produced by using human chromosomes with translocations and deletions.


PCR mapping of somatic cell hybrids is a rapid procedure for assigning a particular sequence to a particular chromosome. Three or more sequences can be assigned per day using a single thermal cycler. Using the NOVX sequences to design oligonucleotide primers, sub-localization can be achieved with panels of fragments from specific chromosomes.


Fluorescence in situ hybridization (FISH) of a DNA sequence to a metaphase chromosomal spread can further be used to provide a precise chromosomal location in one step. Chromosome spreads can be made using cells whose division has been blocked in metaphase by a chemical like colcemid that disrupts the mitotic spindle. The chromosomes can be treated briefly with trypsin, and then stained with Giemsa. A pattern of light and dark bands develops on each chromosome, so that the chromosomes can be identified individually. The FISH technique can be used with a DNA sequence as short as 500 or 600 bases. However, clones larger than 1,000 bases have a higher likelihood of binding to a unique chromosomal location with sufficient signal intensity for simple detection. Preferably 1,000 bases, and more preferably 2,000 bases, will suffice to get good results at a reasonable amount of time. For a review of this technique, see, Verma, et al., HUMAN CHROMOSOMES: A MANUAL OF BASIC TECHNIQUES (Pergamon Press, New York 1988).


Reagents for chromosome mapping can be used individually to mark a single chromosome or a single site on that chromosome, or panels of reagents can be used for marking multiple sites and/or multiple chromosomes. Reagents corresponding to noncoding regions of the genes actually are preferred for mapping purposes. Coding sequences are more likely to be conserved within gene families, thus increasing the chance of cross hybridizations during chromosomal mapping.


Once a sequence has been mapped to a precise chromosomal location, the physical position of the sequence on the chromosome can be correlated with genetic map data. Such data are found, e.g., in McKusick, MENDELIAN INERIANCE IN MAN, available on-line through Johns Hopkins University Welch Medical Library). The relationship between genes and disease, mapped to the same chromosomal region, can then be identified through linkage analysis (co-inheritance of physically adjacent genes), described in, e.g., Egeland, et al., 1987. Nature, 325: 783-787.


Moreover, differences in the DNA sequences between individuals affected and unaffected with a disease associated with the NOVX gene, can be determined. If a mutation is observed in some or all of the affected individuals but not in any unaffected individuals, then the mutation is likely to be the causative agent of the particular disease. Comparison of affected and unaffected individuals generally involves first looking for structural alterations in the chromosomes, such as deletions or translocations that are visible from chromosome spreads or detectable using PCR based on that DNA sequence. Ultimately, complete sequencing of genes from several individuals can be performed to confirm the presence of a mutation and to distinguish mutations from polymorphisms.


Tissue Typing


The NOVX sequences of the invention can also be used to identify individuals from minute biological samples. In this technique, an individual's genomic DNA is digested with one or more restriction enzymes, and probed on a Southern blot to yield unique bands for identification. The sequences of the invention are useful as additional DNA markers for RFLP (“restriction fragment length polymorphisms,” described in U.S. Pat. No. 5,272,057).


Furthermore, the sequences of the invention can be used to provide an alternative technique that determines the actual base-by-base DNA sequence of selected portions of an individual's genome. Thus, the NOVX sequences described herein can be used to prepare two PCR primers from the 5′- and 3′-termini of the sequences. These primers can then be used to amplify an individual's DNA and subsequently sequence it.


Panels of corresponding DNA sequences from individuals, prepared in this manner, can provide unique individual identifications, as each individual will have a unique set of such DNA sequences due to allelic differences. The sequences of the invention can be used to obtain such identification sequences from individuals and from tissue. The NOVX sequences of the invention uniquely represent portions of the human genome. Allelic variation occurs to some degree in the coding regions of these sequences, and to a greater degree in the noncoding regions. It is estimated that allelic variation between individual humans occurs with a frequency of about once per each 500 bases. Much of the allelic variation is due to single nucleotide polymorphisms (SNPs), which include restriction fragment length polymorphisms (RFLts).


Each of the sequences described herein can, to some degree, be used as a standard against which DNA from an individual can be compared for identification purposes. Because greater numbers of polymorphisms occur in the noncoding regions, fewer sequences are necessary to differentiate individuals. The noncoding sequences can comfortably provide positive individual identification with a panel of perhaps 10 to 1,000 primers that each yield a noncoding amplified sequence of 100 bases. If coding sequences, such as those of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, are used, a more appropriate number of primers for positive individual identification would be 500-2,000.


Predictive Medicine


The invention also pertains to the field of predictive medicine in which diagnostic assays, prognostic assays, pharmacogenomics, and monitoring clinical trials are used for prognostic (predictive) purposes to thereby treat an individual prophylactically. Accordingly, one aspect of the invention relates to diagnostic assays for determining NOVX protein and/or nucleic acid expression as well as NOVX activity, in the context of a biological sample (e.g., blood, serum, cells, tissue) to thereby determine whether an individual is afflicted with a disease or disorder, or is at risk of developing a disorder, associated with aberrant NOVX expression or activity. The disorders include metabolic disorders, diabetes, obesity, infectious disease, anorexia, cancer-associated cachexia, cancer, neurodegenerative disorders, Alzheimer's Disease, Parkinson's Disorder, immune disorders, and hematopoietic disorders, and the various dyslipidemias, metabolic disturbances associated with obesity, the metabolic syndrome X and wasting disorders associated with chronic diseases and various cancers. The invention also provides for prognostic (or predictive) assays for determining whether an individual is at risk of developing a disorder associated with NOVX protein, nucleic acid expression or activity. For example, mutations in a NOVX gene can be assayed in a biological sample. Such assays can be used for prognostic or predictive purpose to thereby prophylactically treat an individual prior to the onset of a disorder characterized by or associated with NOVX protein, nucleic acid expression, or biological activity.


Another aspect of the invention provides methods for determining NOVX protein, nucleic acid expression or activity in an individual to thereby select appropriate therapeutic or prophylactic agents for that individual (referred to herein as “pharmacogenomics”). Pharmacogenomics allows for the selection of agents (e.g., drugs) for therapeutic or prophylactic treatment of an individual based on the genotype of the individual (e.g., the genotype of the individual examined to determine the ability of the individual to respond to a particular agent.) Yet another aspect of the invention pertains to monitoring the influence of agents (e.g., drugs, compounds) on the expression or activity of NOVX in clinical trials.


These and other agents are described in further detail in the following sections.


Diagnostic Assays


An exemplary method for detecting the presence or absence of NOVX in a biological sample involves obtaining a biological sample from a test subject and contacting the biological sample with a compound or an agent capable of detecting NOVX protein or nucleic acid (e.g., mRNA, genomic DNA) that encodes NOVX protein such that the presence of NOVX is detected in the biological sample. An agent for detecting NOVX rnRNA or genomic DNA is a labeled nucleic acid probe capable of hybridizing to NOVX mRNA or genomic DNA. The nucleic acid probe can be, for example, a full-length NOVX nucleic acid, such as the nucleic acid of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606, or a portion thereof, such as an oligonucleotide of at least 15, 30, 50, 100, 250 or 500 nucleotides in length and sufficient to specifically hybridize under stringent conditions to NOVX mRNA or genomic DNA. Other suitable probes for use in the diagnostic assays of the invention are described herein.


An agent for detecting NOVX protein is an antibody capable of binding to NOVX protein, preferably an antibody with a detectable label. Antibodies can be polyclonal, or more preferably, monoclonal. An intact antibody, or a fragment thereof (e.g., Fab or F(ab′)2) can be used. The term “labeled”, with regard to the probe or antibody, is intended to encompass direct labeling of the probe or antibody by coupling (i.e., physically linking) a detectable substance to the probe or antibody, as well as indirect labeling of the probe or antibody by reactivity with another reagent that is directly labeled. Examples of indirect labeling include detection of a primary antibody using a fluorescently-labeled secondary antibody and end-labeling of a DNA probe with biotin such that it can be detected with flucrescently-labeled streptavidin. The term “biological sample” is intended to include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject. That is, the detection method of the invention can be used to detect NOVX mRNA, protein, or genomic DNA in a biological sample in vitro as well as in vivo. For example, in vitro techniques for detection of NOVX mRNA include Northern hybridizations and in situ hybridizations. In vitro techniques for detection of NOVX protein include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations, and immunofluorescence. In vitro techniques for detection of NOVX genomic DNA include Southern hybridizations. Furthermore, in vivo techniques for detection of NOVX protein include introducing into a subject a labeled anti-NOVX antibody. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques.


In one embodiment, the biological sample contains protein molecules from the test subject. Alternatively, the biological sample can contain mRNA molecules from the test subject or genomic DNA molecules from the test subject. A preferred biological sample is a peripheral blood leukocyte sample isolated by conventional means from a subject.


In another embodiment, the methods further involve obtaining a control biological sample from a control subject, contacting the control sample with a compound or agent capable of detecting NOVX protein, mRNA, or genomic DNA, such that the presence of NOVX protein, mRNA or genomic DNA is detected in the biological sample, and comparing the presence of NOVX protein, mRNA or genornic DNA in the control sample with the presence of NOVX protein, mRNA or genomic DNA in the test sample.


The invention also encompasses kits for detecting the presence of NOVX in a biological sample. For example, the kit can comprise: a labeled compound or agent capable of detecting NOVX protein or mRNA in a biological sample; means for T determining the amount of NOVX in the sample; and means for comparing the amount of NOVX in the sample with a standard. The compound or agent can be packaged in a suitable container. The kit can further comprise instructions for using the kit to detect NOVX protein or nucleic acid.


Prognostic Assays


The diagnostic methods described herein can furthermore be utilized to identify subjects having or at risk of developing a disease or disorder associated with aberrant NOVX expression or activity. For example, the assays described herein, such as the preceding diagnostic assays or the following assays, can be utilized to identify a subject having or at risk of developing a disorder associated with NOVX protein, nucleic acid expression or activity. Alternatively, the prognostic assays can be utilized to identify a subject having or at risk for developing a disease or disorder. Thus, the invention provides a method for identifying a disease or disorder associated with aberrant NOVX expression or activity in which a test sample is obtained from a subject and NOVX protein or nucleic acid (e.g., mRNA, genornic DNA) is detected, wherein the presence of NOVX protein or nucleic acid is diagnostic for a subject having or at risk of developing a disease or disorder associated with aberrant NOVX expression or activity. As used herein, a “test sample” refers to a biological sample obtained from a subject of interest. For example, a test sample can be a biological fluid (e.g., serum), cell sample, or tissue.


Furthermore, the prognostic assays described herein can be used to determine whether a subject can be administered an agent (e.g., an agonist, antagonist, peptidomimetic, protein, peptide, nucleic acid, small molecule, or other drug candidate) to treat a disease or disorder associated with aberrant NOVX expression or activity. For example, such methods can be used to determine whether a subject can be effectively treated with an agent for a disorder. Thus, the invention provides methods for determining whether a subject can be effectively treated with an agent for a disorder associated with aberrant NOVX expression or activity in which a test sample is obtained and NOVX protein or nucleic acid is detected (e.g., wherein the presence of NOVX protein or nucleic acid is diagnostic for a subject that can be administered the agent to treat a disorder associated with aberrant NOVX expression or activity).


The methods of the invention can also be used to detect genetic lesions in a NOVX gene, thereby determining if a subject with the lesioned gene is at risk for a disorder characterized by aberrant cell proliferation and/or differentiation. In various embodiments, the methods include detecting, in a sample of cells from the subject, the presence or absence of a genetic lesion characterized by at least one of an alteration affecting the integrity of a gene encoding a NOVX-protein, or the misexpression of the NOVX gene. For example, such genetic lesions can be detected by ascertaining the existence of at least one of: (i) a deletion of one or more nucleotides from a NOVX gene; (ii) an addition of one or more nucleotides to a NOVX gene; (iii) a substitution of one or more nucleotides of a NOVX gene, (iv) a chromosomal rearrangement of a NOVX gene; (v) an alteration in the level of a messenger RNA transcript of a NOVX gene, (vi) aberrant modification of a NOVX gene, such as of the methylation pattern of the genomic DNA, (vii) the presence of a non-wild-type splicing pattern of a messenger RNA transcript of a NOVX gene, (viii) a non-wild-type level of a NOVX protein, (ix) allelic loss of a NOVX gene, and (x) inappropriate post-translational modification of a NOVX protein. As described herein, there are a large number of assay techniques known in the art which can be used for detecting lesions in a NOVX gene. A preferred biological sample is a peripheral blood leukocyte sample isolated by conventional means from a subject. However, any biological sample containing nucleated cells may be used, including, for example, buccal mucosal cells.


In certain embodiments, detection of the lesion involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g., U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran, et al., 1988. Science 241: 1077-1080; and Nakazawa, et al., 1994. Proc. Natl. Acad. Sci. USA 91: 360-364), the latter of which can be particularly useful for detecting point mutations in the NOVX-gene (see, Abravaya, et al., 1995. Nucl. Acids Res. 23: 675-682). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers that specifically hybridize to a NOVX gene under conditions such that hybridization and amplification of the NOVX gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. It is anticipated that PCR and/or LCR may be desirable to use as a preliminary amplification step in conjunction with any of the techniques used for detecting mutations described herein.


Alternative amplification methods include: self sustained sequence replication (see, Guatelli, et al., 1990. Proc. Natl. Acad. Sci. USA 87: 1874-1878), transcriptional amplification system (see, Kwoh, et al., 1989. Proc. Natl. Acad. Sci. USA 86: 1173-1177); Qβ Replicase (see, Lizardi, et al, 1988. BioTechnology 6: 1197), or any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art. These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.


In an alternative embodiment, mutations in a NOVX gene from a sample cell can be identified by alterations in restriction enzyme cleavage patterns. For example, sample and control DNA is isolated, amplified (optionally), digested with one or more restriction endonucleases, and fragment length sizes are determined by gel electrophoresis and compared. Differences in fragment length sizes between sample and control DNA indicates mutations in the sample DNA. Moreover, the use of sequence specific ribozymes (see, e.g., U.S. Pat. No. 5,493,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site.


In other embodiments, genetic mutations in NOVX can be identified by hybridizing a sample and control nucleic acids, e.g., DNA or RNA, to high-density arrays containing hundreds or thousands of oligonucleotides probes. See, e.g., Cronin, et al., 1996. Human Mutation 7: 244-255; Kozal, et al., 1996. Nat. Med. 2: 753-759. For example, genetic mutations in NOVX can be identified in two dimensional arrays containing light-generated DNA probes as described in Cronin, et al., supra. Briefly, a first hybridization array of probes can be used to scan through long stretches of DNA in a sample and control to identify base changes between the sequences by making linear arrays of sequential overlapping probes. This step allows the identification of point mutations. This is followed by a second hybridization array that allows the characterization of specific mutations by using smaller, specialized probe arrays complementary to all variants or mutations detected. Each mutation array is composed of parallel probe sets, one complementary to the wild-type gene and the other complementary to the mutant gene.


In yet another embodiment, any of a variety of sequencing reactions known in the art can be used to directly sequence the NOVX gene and detect mutations by comparing the sequence of the sample NOVX with the corresponding wild-type (control) sequence. Examples of sequencing reactions include those based on techniques developed by Maxim and Gilbert, 1977. Proc. Natl. Acad. Sci. USA 74: 560 or Sanger, 1977. Proc. Natl. Acad. Sci. USA 74: 5463. It is also contemplated that any of a variety of automated sequencing procedures can be utilized when performing the diagnostic assays (see, e.g., Naeve, et al., 1995. Biotechniques 19: 448), including sequencing by mass spectrometry (see, e.g., PCT International Publication No. WO 94/16101; Cohen, et al., 1996. Adv. Chromatography 36: 127-162; and Griffin, et al., 1993. Appl. Biochem. Biotechnol. 38: 147-159).


Other methods for detecting mutations in the NOVX gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA heteroduplexes. See, e.g., Myers, et al., 1985. Science 230: 1242. In general, the art technique of “mismatch cleavage” starts by providing heteroduplexes of formed by hybridizing (labeled) RNA or DNA containing the wild-type NOVX sequence with potentially mutant RNA or DNA obtained from a tissue sample. The double-stranded duplexes are treated with an agent that cleaves single-stranded regions of the duplex such as which will exist due to basepair mismatches between the control and sample strands. For instance, RNA/DNA duplexes can be treated with RNase and DNA/DNA hybrids treated with SI nuclease to enzymatically digesting the mismatched regions. In other embodiments, either DNA/DNA or RNA/DNA duplexes can be treated with hydroxylamine or osmium tetroxide and with piperidine in order to digest mismatched regions. After digestion of the mismatched regions, the resulting material is then separated by size on denaturing polyacrylamide gels to determine the site of mutation. See, e.g., Cotton, et al., 1988. Proc. Natl. Acad. Sci. USA 85: 4397; Saleeba, et al., 1992. Methods Enzymol. 217: 286-295. In an embodiment, the control DNA or RNA can be labeled for detection.


In still another embodiment, the mismatch cleavage reaction employs one or more proteins that recognize mismatched base pairs in double-stranded DNA (so called “DNA mismatch repair” enzymes) in defined systems for detecting and mapping point mutations in NOVX cDNAs obtained from samples of cells. For example, the mutY enzyme of E. coli cleaves A at G/A mismatches and the thymidine DNA glycosylase from HeLa cells cleaves T at G/T mismatches. See, e.g., Hsu, et al., 1994. Carcinogenesis 15: 1657-1662. According to an exemplary embodiment, a probe based on a NOVX sequence, e.g., a wild-type NOVX sequence, is hybridized to a cDNA or other DNA product from a test cell(s). The duplex is treated with a DNA mismatch repair enzyme, and the cleavage products, if any, can be detected from electrophoresis protocols or the like. See, e.g., U.S. Pat. No. 5,459,039.


In other embodiments, alterations in electrophoretic mobility will be used to identify mutations in NOVX genes. For example, single strand conformation polymorphism (SSCP) may be used to detect differences in electrophoretic mobility between mutant and wild type nucleic acids. See, e.g., Orita, et al., 1989. Proc. Natl. Acad. Sci. USA: 86: 2766; Cotton, 1993. Mutat. Res. 285: 125-144; Hayashi, 1992. Genet. Anal. Tech. Appl. 9: 73-79. Single-stranded DNA fragments of sample and control NOVX nucleic acids will be denatured and allowed to renature. The secondary structure of single-stranded nucleic acids varies according to sequence, the resulting alteration in electrophoretic mobility enables the detection of even a single base change. The DNA fragments may be labeled or detected with labeled probes. The sensitivity of the assay may be enhanced by using RNA (rather than DNA), in which the secondary structure is more sensitive to a change in sequence. In one embodiment, the subject method utilizes heteroduplex analysis to separate double stranded heteroduplex molecules on the basis of changes in electrophoretic mobility. See, e.g., Keen, et al., 1991. Trends Genet. 7: 5.


In yet another embodiment, the movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (DGGE). See, e.g., Myers, et al., 1985. Nature 313: 495. When DGGE is used as the method of analysis, DNA will be modified to insure that it does not completely denature, for example by adding a GC clamp of approximately 40 bp of high-melting GC-rich DNA by PCR. In a further embodiment, a temperature gradient is used in place of a denaturing gradient to identify differences in the mobility of control and sample DNA. See, e.g., Rosenbaum and Reissner, 1987. Biophys. Chem. 265: 12753.


Examples of other techniques for detecting point mutations include, but are not limited to, selective oligonucleotide hybridization, selective amplification, or selective primer extension. For example, oligonucleotide primers may be prepared in which the known mutation is placed centrally and then hybridized to target DNA under conditions that permit hybridization only if a perfect match is found. See, e.g., Saiki, et al., 1986. Nature 324: 163; Saiki, et al., 1989. Proc. Natl. Acad. Sci. USA 86: 6230. Such allele specific oligonucleotides are hybridized to PCR amplified target DNA or a number of different mutations when the oligonucleotides are attached to the hybridizing membrane and hybridized with labeled target DNA.


Alternatively, allele specific amplification technology that depends on selective PCR amplification may be used in conjunction with the instant invention. Oligonucleotides used as primers for specific amplification may carry the mutation of interest in the center of the molecule (so that amplification depends on differential hybridization; see, e.g., Gibbs, et al., 1989. Nucl. Acids Res. 17: 2437-2448) or at the extreme 3′-terminus of one primer where, under appropriate conditions, mismatch can prevent, or reduce polymerase extension (see, e.g., Prossner, 1993. Tibtech. 11: 238). In addition it may be desirable to introduce a novel restriction site in the region of the mutation to create cleavage-based detection. See, e.g., Gasparini, et al., 1992. Mol. Cell Probes 6: 1. It is anticipated that in certain embodiments amplification may also be performed using Taq ligase for amplification. See, e.g., Barany, 1991. Proc. Natl. Acad. Sci. USA 88: 189. In such cases, ligation will occur only if there is a perfect match at the 3′-terminus of the 5′ sequence, making it possible to detect the presence of a known mutation at a specific site by looking for the presence or absence of amplification.


The methods described herein may be performed, for example, by utilizing pre-packaged diagnostic kits comprising at least one probe nucleic acid or antibody reagent described herein, which may be conveniently used, e.g., in clinical settings to diagnose patients exhibiting symptoms or family history of a disease or illness involving a NOVX gene.


Furthermore, any cell type or tissue, preferably peripheral blood leukocytes, in which NOVX is expressed may be utilized in the prognostic assays described herein. However, any biological sample containing nucleated cells may be used, including, for example, buccal mucosal cells.


Pharmacogenonics


Agents, or modulators that have a stimulatory or inhibitory effect on NOVX activity (e.g., NOVX gene expression), as identified by a screening assay described herein can be administered to individuals to treat (prophylactically or therapeutically) disorders. The disorders include but are not limited to, e.g., those diseases, disorders and conditions listed above, and more particularly include those diseases, disorders, or conditions associated with homologs of a NOVX protein, such as those summarized in Table A.


In conjunction with such treatment, the pharmacogenomics (i.e., the study of the relationship between an individual's genotype and that individual's response to a foreign compound or drug) of the individual may be considered. Differences in metabolism of therapeutics can lead to severe toxicity or therapeutic failure by altering the relation between dose and blood concentration of the pharmacologically active drug. Thus, the pharmacogenomics of the individual permits the selection of effective agents (e.g., drugs) for prophylactic or therapeutic treatments based on a consideration of the individual's genotype. Such pharmacogenomics can further be used to determine appropriate dosages and therapeutic regimens. Accordingly, the activity of NOVX protein, expression of NOVX nucleic acid, or mutation content of NOVX genes in an individual can be determined to thereby select appropriate agent(s) for therapeutic or prophylactic treatment of the individual.


Pharmacogenomics deals with clinically significant hereditary variations in the response to drugs due to altered drug disposition and abnormal action in affected persons. See e.g., Eichelbaum, 1996. Clin. Exp. Pharmacol. Physiol., 23: 983-985; Linder, 1997. Clin. Chem., 43: 254-266. In general, two types of pharmacogenetic conditions can be differentiated. Genetic conditions transmitted as a single factor altering the way drugs act on the body (altered drug action) or genetic conditions transmitted as single factors altering the way the body acts on drugs (altered drug metabolism). These pharmacogenetic conditions can occur either as rare defects or as polymorphisms. For example, glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common inherited enzymopathy in which the main clinical complication is hemolysis after ingestion of oxidant drugs (anti-malarials, sulfonamides, analgesics, nitrofurans) and consumption of fava beans.


As an illustrative embodiment, the activity of drug metabolizing enzymes is a major determinant of both the intensity and duration of drug action. The discovery of genetic polymorphisms of drug metabolizing enzymes (e.g., N-acetyltransferase 2 (NAT 2) and cytochrome pregnancy zone protein precursor enzymes CYP2D6 and CYP2C19) has provided an explanation as to why some patients do not obtain the expected drug effects or show exaggerated drug response and serious toxicity after taking the standard and safe dose of a drug. These polymorphisms are expressed in two phenotypes in the population, the extensive metabolizer (EM) and poor metabolizer (PM). The prevalence of PM is different among different populations. For example, the gene coding for CYP2D6 is highly polymorphic and several mutations have been identified in PM, which all lead to the absence of functional CYP2D6. Poor metabolizers of CYP2D6 and CYP2C19 quite frequently experience exaggerated drug response and side effects when they receive standard doses. If a metabolite is the active therapeutic moiety, PM show no therapeutic response, as demonstrated for the analgesic effect of codeine mediated by its CYP2D6-formed metabolite morphine. At the other extreme are the so called ultra-rapid metabolizers who do not respond to standard doses. Recently, the molecular basis of ultra-rapid metabolism has been identified to be due to CYP2D6 gene amplification.


Thus, the activity of NOVX protein, expression of NOVX nucleic acid, or mutation content of NOVX genes in an individual can be determined to thereby select appropriate agent(s) for therapeutic or prophylactic treatment of the individual. In addition, pharmacogenetic studies can be used to apply genotyping of polymorphic alleles encoding drug-metabolizing enzymes to the identification of an individual's drug responsiveness phenotype. This knowledge, when applied to dosing or drug selection, can avoid adverse reactions or therapeutic failure and thus enhance therapeutic or prophylactic efficiency when treating a subject with a NOVX modulator, such as a modulator identified by one of the exemplary screening assays described herein.


Monitoring of Effects During Clinical Trials


Monitoring the influence of agents (e.g., drugs, compounds) on the expression or activity of NOVX (e.g., the ability to modulate aberrant cell proliferation and/or differentiation) can be applied not only in basic drug screening, but also in clinical trials. For example, the effectiveness of an agent determined by a screening assay as described herein to increase NOVX gene expression, protein levels, or upregulate NOVX activity, can be monitored in clinical trails of subjects exhibiting decreased NOVX gene expression, protein levels, or downregulated NOVX activity. Alternatively, the effectiveness of an agent determined by a screening assay to decrease NOVX gene expression, protein levels, or downregulate NOVX activity, can be monitored in clinical trails of subjects exhibiting increased NOVX gene expression, protein levels, or upregulated NOVX activity. In such clinical trials, the expression or activity of NOVX and, preferably, other genes that have been implicated in, for example, a cellular proliferation or immune disorder can be used as a “read out” or markers of the immune responsiveness of a particular cell.


By way of example, and not of limitation, genes, including NOVX, that are modulated in cells by treatment with an agent (e.g., compound, drug or small molecule) that modulates NOVX activity (e.g., identified in a screening assay as described herein) can be identified. Thus, to study the effect of agents on cellular proliferation disorders, for example, in a clinical trial, cells can be isolated and RNA prepared and analyzed for the levels of expression of NOVX and other genes implicated in the disorder. The levels of gene expression (i.e., a gene expression pattern) can be quantified by Northern blot analysis or RT-PCR, as described herein, or alternatively by measuring the amount of protein produced, by one of the methods as described herein, or by measuring the levels of activity of NOVX or other genes. In this manner, the gene expression pattern can serve as a marker, indicative of the physiological response of the cells to the agent. Accordingly, this response state may be determined before, and at various points during, treatment of the individual with the agent.


In one embodiment, the invention provides a method for monitoring the effectiveness of treatment of a subject with an agent (e.g., an agonist, antagonist, protein, peptide, peptidomimetic, nucleic acid, small molecule, or other drug candidate identified by the screening assays described herein) comprising the steps of (i) obtaining a pre-administration sample from a subject prior to administration of the agent; (ii) detecting the level of expression of a NOVX protein, mRNA, or genomic DNA in the preadministration sample; (iii) obtaining one or more post-admninistration samples from the subject; (iv) detecting the level of expression or activity of the NOVX protein, mRNA, or genomic DNA in the post-administration samples; (v) comparing the level of expression or activity of the NOVX protein, mRNA, or genomic DNA in the pre-administration sample with the NOVX protein, mRNA, or genomic DNA in the post administration sample or samples; and (vi) altering the administration of the agent to the subject accordingly. For example, increased administration of the agent may be desirable to increase the expression or activity of NOVX to higher levels than detected, i.e., to increase the effectiveness of the agent. Alternatively, decreased administration of the agent may be desirable to decrease expression or activity of NOVX to lower levels than detected, i.e., to decrease the effectiveness of the agent.


Methods of Treatment


The invention provides for both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with aberrant NOVX expression or activity. The disorders include but are not limited to, e.g., those diseases, disorders and conditions listed above, and more particularly include those diseases, disorders, or conditions associated with homologs of a NOVX protein, such as those summarized in Table A.


These methods of treatment will be discussed more fully, below.


Diseases and Disorders


Diseases and disorders that are characterized by increased (relative to a subject not suffering from the disease or disorder) levels or biological activity may be treated with Therapeutics that antagonize (i.e., reduce or inhibit) activity. Therapeutics that antagonize activity may be administered in a therapeutic or prophylactic manner. Therapeutics that may be utilized include, but are not limited to: (i) an aforementioned peptide, or analogs, derivatives, fragments or homologs thereof; (ii) antibodies to an aforementioned peptide; (iii) nucleic acids encoding an aforementioned peptide; (iv) administration of antisense nucleic acid and nucleic acids that are “dysfunctional” (i.e., due to a heterologous insertion within the coding sequences of coding sequences to an aforementioned peptide) that are utilized to “knockout” endogenous function of an aforementioned peptide by homologous recombination (see, e.g., Capecchi, 1989. Science 244: 1288-1292); or (ν) modulators ( i.e., inhibitors, agonists and antagonists, including additional peptide mimetic of the invention or antibodies specific to a peptide of the invention) that alter the interaction between an aforementioned peptide and its binding partner.


Diseases and disorders that are characterized by decreased (relative to a subject not suffering from the disease or disorder) levels or biological activity may be treated with Therapeutics that increase (i.e., are agonists to) activity. Therapeutics that upregulate activity may be administered in a therapeutic or prophylactic manner. Therapeutics that may be utilized include, but are not limited to, an aforementioned peptide, or analogs, derivatives, fragments or homologs thereof; or an agonist that increases bioavailability.


Increased or decreased levels can be readily detected by quantifying peptide and/or RNA, by obtaining a patient tissue sample (e.g., from biopsy tissue) and assaying it in vitro for RNA or peptide levels, structure and/or activity of the expressed peptides (or mRNAs of an aforementioned peptide). Methods that are well-known within the art include, but are not limited to, immunoassays (e.g., by Western blot analysis, immunoprecipitation followed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, immunocytochemistry, etc.) and/or hybridization assays to detect expression of mRNAs (e.g., Northern assays, dot blots, in situ hybridization, and the like).


Prophylactic Methods


In one aspect, the invention provides a method for preventing, in a subject, a disease or condition associated with an aberrant NOVX expression or activity, by administering to the subject an agent that modulates NOVX expression or at least one NOVX activity. Subjects at risk for a disease that is caused or contributed to by aberrant NOVX expression or activity can be identified by, for example, any or a combination of diagnostic or prognostic assays as described herein. Administration of a prophylactic agent can occur prior to the manifestation of symptoms characteristic of the NOVX aberrancy, such that a disease or disorder is prevented or, alternatively, delayed in its progression. Depending upon the type of NOVX aberrancy, for example, a NOVX agonist or NOVX antagonist agent can be used for treating the subject. The appropriate agent can be determined based on screening assays described herein. The prophylactic methods of the invention are further discussed in the following subsections.


Therapeutic Methods


Another aspect of the invention pertains to methods of modulating NOVX expression or activity for therapeutic purposes. The modulatory method of the invention involves contacting a cell with an agent that modulates one or more of the activities of NOVX protein activity associated with the cell. An agent that modulates NOVX protein activity can be an agent as described herein, such as a nucleic acid or a protein, a naturally-occurring cognate ligand of a NOVX protein, a peptide, a NOVX peptidomimetic, or other small molecule. In one embodiment, the agent stimulates one or more NOVX protein activity. Examples of such stimulatory agents include active NOVX protein and a nucleic acid molecule encoding NOVX that has been introduced into the cell. In another embodiment, the agent inhibits one or more NOVX protein activity. Examples of such inhibitory agents include antisense NOVX nucleic acid molecules and anti-NOVX antibodies. These modulatory methods can be performed in vitro (e.g., by culturing the cell with the agent) or, alternatively, in vivo (e.g., by administering the agent to a subject). As such, the invention provides methods of treating an individual afflicted with a disease or disorder characterized by aberrant expression or activity of a NOVX protein or nucleic acid molecule. In one embodiment, the method involves administering an agent (e.g., an agent identified by a screening assay described herein), or combination of agents that modulates (e.g., up-regulates or down-regulates) NOVX expression or activity. In another embodiment, the method involves administering a NOVX protein or nucleic acid molecule as therapy to compensate for reduced or aberrant NOVX expression or activity.


Stimulation of NOVX activity is desirable in situations in which NOVX is abnormally downregulated and/or in which increased NOVX activity is likely to have a beneficial effect. One example of such a situation is where a subject has a disorder characterized by aberrant cell proliferation and/or differentiation (e.g., cancer or immune associated disorders). Another example of such a situation is where the subject has a gestational disease (e.g., preclampsia).


Determination of the Biological Effect of the Therapeutic


In various embodiments of the invention, suitable in vitro or in vivo assays are performed to determine the effect of a specific Therapeutic and whether its administration is indicated for treatment of the affected tissue.


In various specific embodiments, in vitro assays may be performed with representative cells of the type(s) involved in the patient's disorder, to determine if a given Therapeutic exerts the desired effect upon the cell type(s). Compounds for use in therapy may be tested in suitable animal model systems including, but not limited to rats, mice, chicken, cows, monkeys, rabbits, and the like, prior to testing in human subjects. Similarly, for in vivo testing, any of the animal model system known in the art may be used prior to administration to human subjects.


Prophylactic and Therapeutic Uses of the Compositions of the Invention


The NOVX nucleic acids and proteins of the invention are useful in potential prophylactic and therapeutic applications implicated in a variety of disorders. The disorders include but are not limited to, e.g., those diseases, disorders and conditions listed above, and more particularly include those diseases, disorders, or conditions associated with homologs of a NOVX protein, such as those summarized in Table A.


As an example, a cDNA encoding the NOVX protein of the invention may be useful in gene therapy, and the protein may be useful when administered to a subject in need thereof. By way of non-limiting example, the compositions of the invention will have efficacy for treatment of patients suffering from diseases, disorders, conditions and the like, including but not limited to those listed herein.


Both the novel nucleic acid encoding the NOVX protein, and the NOVX protein of the invention, or fragments thereof, may also be useful in diagnostic applications, wherein the presence or amount of the nucleic acid or the protein are to be assessed. A further use could be as an anti-bacterial molecule (i.e., some peptides have been found to possess anti-bacterial properties). These materials are further useful in the generation of antibodies, which immunospecifically-bind to the novel substances of the invention for use in therapeutic or diagnostic methods.


The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims.


EXAMPLES
Example A
Polynucleotide and Polypeptide Sequences, and Homology Data
Example 1

The NOV1clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 1A.

TABLE 1ANOV1 Sequence AnalysisNOV1a, CG191083-01SEQ ID NO: 14494 bpDNA SequenceORF Start: ATG at 59ORF Stop: TAA at 1394CGAGAGCGGGAGTGAGCGAGCGAGCGAGTTGCCGAGCGCGCCCCGTCCCTCGCGCGCGATGCTCCCCTGGACGGCGCTCGGCCTGGCCCTGAGCTTGCGGCTGGCGCTGGCGCGGAGCGGCGCAGAGCGCGGTCCACCAGCATCAGCCCCCCGAGGGGACCTGATGTTCCTGCTGGACAGCTCAGCCAGCGTCTCTCACTACGAGTTCTCCCGGGTTCGGGAGTTTGTGGGGCAGCTGGTGGCTCCACTGCCCCTGGGCACCGGGGCCCTGCGTGCCAGTCTGGTGCACGTGGGCAGTCGGCCATACACCGAGTTCCCCTTCGGCCAGCACAGCTCGGGTGAGGCTGCCCAGGATGCGGTGCGTGCTTCTGCCCAGCGCATGGGTGACACCCACACTGGCCTGGCGCTGGTCTATGCCAAGGAACAGCTGTTTGCTGAAGCATCAGGTGCCCGGCCAGGGGTGCCCAAAGTGCTGGTGTGGGTGACAGATGGCGGCTCCAGCGACCCTGTGGGCCCCCCCATGCAGGAGCTCAAGGACCTGGGCGTCACCGTGTTCATTGTCAGCACCGGCCGAGGCAACTTCCTGGAGCTGTCAGCCGCTGCCTCAGCCCCTGCCGAGAAGCACCTGCACTTTGTGGACGTGGATGACCTGCACATCATTGTCCAAGAGCTGAGGGGCTCCATTCTCGACGCGATGCGGCCGCAGCAGCTCCATGCCACGGAGATCACGTCCAGCGGCTTCCGCCTGGCCTGGCCACCCCTGCTGACCGCAGACTCGGGCTACTATGTGCTGGAGCTGGTGCCCAGCGCCCAGCCGGGGGCTGCAAGACGCCAGCAGCTGCCAGGGAACGCCACGGACTGGATCTGGGCCGGCCTCGACCCGGACACGGACTACGACGTGGCGCTAGTGCCTGAGTCCAACGTGCGCCTCCTGAGGCCCCAGATCCTGCGGGTGCGCACGCGGCCCGGTGAGGCAGGGCCGGGGGCTTCGGGCCCGGAGTCGGGGGCTGGGCCGGCCCCCACGCAGCTCGCCGCCCTCCCCGCCCCAGAGGAGGCCGGGCCAGAGCGCATCGTCATCTCCCACGCCCGGCCGCGCAGCCTCCGCGTGAGTTGGGCCCCAGCGCTGGGCTCAGCCGCGGCGCTCGGCTACCACGTGCAGTTCGGGCCGCTGCGGGGCGGGGAGGCGCAGCGGGTGGAGGTGCCCGCGGGCCGCAACTGCACCACGCTGCAGGGCCTGGCGCCGGGCACCGCCTACCTGGTGACCGTGACCGCCGCCTTCCGCTCGGGCCGCGAGAGCGCGCTGTCCGCCAAGGCCTGCACGCCCGACGGCCCGCGCCCGCGCCCACGCCCCGTGCCCCGCGCCCCGACCCCGGGGACCGCCAGCCGTGAGCCGTAAGCCGGCGTCCCCGCCCAGCCGAGAGGGCCGGCGCCTACCTGAGGGCCCCTGTGTCCCGAACCCGGAGCGGAGGCGCCCAACCCGGCAGACGGGTGCAGGCCCGGCCTTTCCCCACGCGGACTCCGCGCGACCCCGGCCCTCTCCCTGCGGCCGCAGGGCTTCCCCGCCTGGCGCCTGCCCTCCAGGGCTGGGGCCTCGCCTGGCGGGACCCCGCAGCAGCCCCGGCCCCATCCCCCCCCAGAGCCGGGCGTCGTGTGGGTCCGTGGGTGATAATTGAGAGCGTCAGACCCAGGACTGTTCAGGCCAGCTTGGGTCCTGGACGTTGATAGGAAGCGGAAGGGGAATCGCGGGAAGCTGGCCCAGGTCAGGTCCGCAAAGGCTTCTGAAGAAGAGGAAGGGCGAGTAGGGGCACCTGGACGCTGATGGTGGCCAGGATGCTCAGCTGGCCAGGAGGGCAGCACCTGCTGGGGACGGTGGCCCTGCCTTCATGCCCAGGACACCAGCTGGGTCCAGCTAGCAGCCACTGGGAATCAGAGGAATGGGGCAGAGCTGGCCATTCAGGACCTTGAGGACAGGTGACCCCACCCGCCCACCGCCACTATCAGGCCCCGGGACCGCACTCACAGGAAACCTTCCGTCGTGACGGAGCACTTCCCAGGGGCCGCAGGGACGACACTCTCCAGGGAGGCCCCAGCAACCACACCATCTTCTTGCTGTGAGAGGTCTCACCCCGGGCTACCTCCTGTCACTACTCACTGCCCTGGGGTCCGTGGGCAAGTTGCCCAGGGTGGGGGTGCCTAGCCAGGTGCAGTCCCCGCCCCGCCTAGTCCTCGGCGTCACGCAATGCTCACCTCGCCTCTTCCCCACTAACATCCCAGACTTTAAAATTCAGTAAATCAGATGTACACCGAGCCTCCAGTCTCGCTAGGATGCTTTACTCCTCCACAAACAGCAGCCGCCGACAGGACAGCTTGTGAACGTCTCCGCCTTTTGGGGTGTCCGGGGAGAGGAGGTGGAGCTGTCCCCTCCCAGTGTCAGGCCTCCTTGGTCCTCATGTGCACCTTTTATTCAGAGAGCTTGGGCCCCCTTCCTTTGGAGCTGGGCCCAGGGCAGGTGGCTGGAATGCAGAGGACAAGGGGGCTGGGGCCTCTGCAGGGCCATTCCCACCGCAGTCTCTGCCACGGCCCGGAGCCCTGGGCCCAGGGCCACGCGGGCTCCCTCTGCTGGCAAAGCCACACCCTGCACCGCATGGGGTCCACTGCCCCTTTCCCCACGTGGACTCTGCGTGACCCCAGGAACTGCAGCATTGAGGTGGTCTCAGTCCCTCCCTCAGGTCTGGTGGGCATTGTGCCAGGGGCCACCTTCGGGACAGCTGGCCTTGGCACCGTCTCCCAAGAACCCAGCTCTGTGCATCCCGTGGCCCCACCAGAAACCCAAGGCCACACCGCCCACCAGCTGGGTGTGGTCACCATTCATGGAGGGCCGGCTGGGGCCAGGCTCACCACGCAGAGTTTGCCCTCCGGTGTGATGATGCATGAGACACAGTAGGCCCCCCTGCCTGTGTGTGCAGGGGGTGTCTGTGGGGGGGCCCTGCTGGCTGGACTTTCGGTTTCTCCTGAGCTCCCCTTAGCCCCCAAGTCAGGCCACGGAGGCCTGAGTAGGACCCATGGGCTGTGTGTGTCACTGCAGGTGGCGTGCTCACAACTGCACTGCTGGTCGGCAGGTGGCCAGGGTTGCAGGCCCGGGCCTCGGGCCCCACCTCCTCTGGAGGGGTAGGATCTCCTTCGTGCAGCCCCTGCCCCCTACCCTGTTCTCCGGAGTGTTGGCAGATCTGAGCCCACGGTCACGTGAGAGGAGATGCCTCTTGCACACTGAGCCCAGGCCCAGCTCACGGAGCACCCTGCAGGTGGCAGCGGGGCACCAGGGGCTCATTCCTGGTGGCCTCAGTGGCTTCTGTGGACGACTCAGACTCACATGAGAAGCTGGGAGGAGCTCCAGCTCTGCAATCCCGAGAGGGCAGAGCGGGGGCCCTGGCCACAACCCTGCTCCCCACATACCCGTCTGGCAGAGGCTCTGCCTCCCCTCTGCCCATCTCTGCACACCAAGGCTGAGCACAGCACAAGGCCTCACGGAGAGGGTGAAAAGGCACTGCCGTCTCCATCTCAGTGCCAAGGAATGCCCCTTTCAGAAGCCCTCGTCTGAGAAGTGCTGCCCCTTATGTAGCCCCAGGCATTTTAAAATCTTTGAATCTGCACCAAGTCTCAGAACAGCTCTGGCCCCGGAGTGCTCCGTCCAGGCCGCTGTGAACCGGCTGTCCCCGCGTCTCCTCCACCTCCCCTGACACAATCCTGGCCCCGACTCAGTCCACCCAGGGTGCAGTGCAGAGGCTGATACCCGCCAGGACTTCCTTGCCAAGGGCCCGGCCACGTCACAAGGCCACTGCACGCTTTTCGACATGCACCTGGAAATCGGGAAGGGCCATGCTGCAGTTGCTTCTAGTTGAGACGAGAAGAGCGACAAGGTTGTGATCCACGTGGCAGGTGTTCAGAAGGCTGGGGGCGGGCAGCGCTGGGGAGAGCCCTGGGTACTTCGAGGAGACCCCGAAGGGAGGCTGCTCCCACACCTGCGCCAGTTTCCACCCTCTCTGTGAGCAGGGCTGCGGTCACCTCCCACATCTGAAGAGAACCAACCTGAGGATTTCACGCTGGCTGCGTGCCAGACCAGTCCCTGACAGGTTGTGCGAGGCCCTTCGCTGGACAGCCCATTGCTGGCCACTGGACGGAGAGGCAGAGGGGGCTGAAATTCGGGCCCATGCCTCTGTGAGCGATGACGGAGCAACAGCTCTCCAGCACGTGAAGCTCTCCAGACAGCTGTTCGTGAGAAGCCAGACAGAGGCCTGGGGTCTCAGTCCAGATTTCTGGGGAGTGGGGTGTCCAAGCGTGGGCCACGCTGCTGGGAGCCACCTAGGGAAGCAGGTCGCCTGTTTCTATAGTGACGGTAATAAACCAAGTCAATTTTCTATAAAAAAAANOV1a, CG191083-01SEQ ID NO: 2445 aaMW at 46803.5kDProtein SequenceMLPWTALGLALSLRLALARSGAERGPPASAPRGDLMFLLDSSASVSHYEFSRVREFVGQLVAPLPLGTGALRASLVHVGSRPYTEFPFGQHSSGEAAQDAVRASAQRMGDTHTGLALVYAKEQLFAEASGARPGVPKVLVWVTDGGSSDPVGPPMQELKDLGVTVFIVSTGRGNFLELSAAASAPAEKHLHFVDVDDLHIIVQELRGSILDAMRPQQLHATEITSSGFRLAWPPLLTADSGYYVLELVPSAQPGAARRQQLPGNATDWIWAGLDPDTDYDVALVPESNVRLLRPQILRVRTRPGEAGPGASGPESGAGPAPTQLAALPAPEEAGPERIVIHARPRSLRVSWAPALGSAAALGYHVQFGPLRGGEAQRVEVPAGPNCTTLQGLAPGTAYLVTVTAAFRSGRESALSAKACTPDGPRPRPRPVPRAPTPGTASREP


Further analysis of the NOV1a protein yielded the following properties shown in Table 1B.

TABLE 1BProtein Sequence Properties NOV1aSignalP analysis:Cleavage site between residues 19 and 20PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 13; peak value 8.36PSG score:3.96GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 2.40possible cleavage site: between 18 and 19>>> Seems to have a cleavable signal peptide (1 to 18)ALOM:Klein et al's method for TM region allocationInit position for calculation: 19Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 2.17 (at 60)ALOM score: 2.17 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 9Charge difference: 1.0 C(2.0)-N(1.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptideMITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):2.90Hyd Moment(95):1.31G content:2D/E content:1S/T content:3Score: −4.22Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 29 ARS|GANUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 9.2%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):33.3%: extracellular, including cell wall33.3%: mitochondrial22.2%: vacuolar11.1%: endoplasmic reticulum>> prediction for CG191083-01 is exc (k = 9)


A search of the NOV1a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 1C.

TABLE 1CGeneseq Results for NOV1aIdentities/NOV1aSimilaritiesGeneseqProtein/Organism/LengthResidues/for theExpectIdentifier[Patent #, Date]Match ResiduesMatched RegionValueABP69674Human polypeptide SEQ ID NO 1721 -1 . . . 445445/445 (100%)0.0Homo sapiens, 445 aa.1 . . . 445445/445 (100%)[WO200270539-A2, 12-SEP-2002]AAE32502Human Willebrand Factor A domain1 . . . 445418/445 (93%)0.0related-protein (WARP) - Homo1 . . . 418418/445 (93%)sapiens, 418 aa. [WO200288184-A1,07-NOV-2002]AAE32501Mouse Willebrand Factor A domain1 . . . 445324/445 (72%)0.0related-protein (WARP) - Mus sp, 4151 . . . 415356/445 (79%)aa. [WO200288184-A1, 07-NOV-2002]AAW86326Kidney injury associated molecule1 . . . 445325/445 (73%)e−179HW059 protein - Rattus sp, 421 aa.7 . . . 421356/445 (79%)[WO9853071-A1, 26-NOV-1998]AAB42581Human ORFX ORF2345 polypeptide3 . . . 296274/294 (93%)e−150sequence SEQ ID NO: 4690 - Homo10 . . . 299 274/294 (93%)sapiens, 299 aa. [WO200058473-A2,05-OCT-2000]


In a BLAST search of public sequence databases, the NOV1a protein was found to have homology to the proteins shown in the BLASTP data in Table 1D.

TABLE 1DPublic BLASTP Results for NOV1aIdentities/ProteinNOV1aSimilaritiesAccessionResidues/for theExpectNumberProtein/Organism/LengthMatch ResiduesMatched PortionValueQ8R2Z5Hypothetical protein (VON1 . . . 445325/445 (73%)e−180WILLEBRAND factor A-related1 . . . 415356/445 (79%)protein homolog) - Mus musculus(Mouse), 415 aa.Q8C0Q7VON WILLEBRAND factor A-related1 . . . 445324/445 (72%)e−180protein homolog - Mus musculus1 . . . 415355/445 (78%)(Mouse), 415 aa.Q923K3Von Willebrand factor A-related1 . . . 445324/445 (72%)e−180protein - Mus musculus (Mouse),1 . . . 415356/445 (79%)415 aa.CAC39705Sequence 47 from Patent EP1067182 -1 . . . 257253/257 (98%)e−142Homo sapiens (Human), 285 aa.1 . . . 257254/257 (98%)Q9H6J5Hypothetical protein FLJ22215 -213 . . . 445 233/233 (100%)e−134Homo sapiens (Human), 233 aa.1 . . . 233233/233 (100%)


PFam analysis predicts that the NOV1a protein contains the domains shown in the Table 1E.

TABLE 1EDomain Analysis of NOV1aIdentities/SimilaritiesPfamfor theDomainNOV1a Match RegionMatched RegionExpect Valuevwa34 . . . 20568/194(35%)1.5e−40132/194(68%)fn3212 . . . 28722/85(26%)0.1852/85(61%)fn3332 . . . 41330/85(35%)1.4e−1153/85(62%)


Example 2

The NOV2 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 2A.

TABLE 2ANOV2 Sequence AnalysisNov2a, CG191745-01SEQ ID NO: 33462 bpDNA SequenceORF Start: ATG at 2ORF Stop: TGA at 3443GATGGGGCCAGAACGGACAGGGGCCGCGCCGCTGCCGCTGCTGCTGGTGTTAGCGCTCAGTCAAGGCATTTTAAATTGTTGTTTGGCCTACAATGTTGGTCTCCCAGAAGCAAAAATATTTTCCGGTCCTTCAAGTGAACAGTTTGGCTATGCAGTGCAGCAGTTTATAAATCCAAAAGGCAACTGGTTACTGGTTGGTTCACCCTGGAGTGGCTTTCCTGAGAACCGAATGGGAGATGTGTATAAATGTCCTGTTGACCTATCCACTGCCACATGTGAAAAACTAAATTTGCAAACTTCAACAAGCATTCCAAATGTTACTGAGATGAAAACCAACATGAGCCTCGGCTTGATCCTCACCAGGAACATGGGAACTGGAGGTTTTCTCACATGTGGTCCTCTGTGGGCACAGCAATGTGGGAATCAGTATTACACAACGGGTGTGTGTTCTGACATCAGTCCTGATTTTCAGCTCTCAGCCAGCTTCTCACCTGCAACTCAGCCCTGCCCTTCCCTCATAGATGTTGTGGTTGTGTGTGATGAATCAAATAGTATTTATCCTTGGGATGCAGTAAAGAATTTTTTGGAAAAATTTGTACAAGGCCTGGATATAGGCCCCACAAAGACACAGGTGGGGTTAATTCAGTATGCCAATAATCCAAGAGTTGTGTTTAACTTGAACACATATAAAACCAAAGAAGAAATGATTGTAGCAACATCCCAGACATCCCAATATGGTGGGGACCTCACAAACACATTCGGAGCAATTCAATATGCAAGAAAATATGCTTATTCAGCAGCTTCTGGTGGGCGACGAAGTGCTACGAAAGTAATGGTAGTTGTAACTGACGGTGAATCACATGATGGTTCAATGTTGAAAGCTGTGATTGATCAATGCAACCATGACAATATACTGAGGTTTGGCATAGCAGTTCTTGGGTACTTAAACAGAAACGCCCTTGATACTAAAAATTTAATAAAAGAAATAAAAGCAATCGCTAGTATTCCAACAGAAAGATACTTTTTCAATGTGTCTGATGAAGCAGCTCTACTAGAAAAGGCTGGGACATTAGGAGAACAAATTTTCAGCATTGAAGGTACTGTTCAAGGAGGAGACAACTTTCAGATGGAAATGTCACAAGTGGGATTCAGTGCAGATTACTCTTCTCAAAATGATATTCTGATGCTGGGTGCAGTGGGAGCTTTTGGCTGGAGTGGGACCATTGTCCAGAAGACATCTCATGGCCATTTGATCTTTCCTAAACAAGCCTTTGACCAAATTCTGCAGGACAGAAATCACAGTTCATATTTAGGTTACTCTGTGGCTGCAATTTCTACTGGAGAAAGCACTCACTTTGTTGCTGGTGCTCCTCGGGCAAATTATACCGGCCAGATAGTGCTATATAGTGTGAATGAGAATGGCAATATCACGGTTATTCAGGCTCACCGAGGTGACCAGATTGGCTCCTATTTTGGTAGTGTGCTGTGTTCAGTTGATGTGGATAAAGACACCATTACAGACGTGCTCTTGGTAGGTGCACCAATGTACATGAGTGACCTAAAGAAAGAGGAAGGAAGAGTCTACCTGTTTACTATCAAAGAGGGCATTTTGGGTCAGCACCAATTTCTTGAAGGCCCCGAGGGCATTGAAAACACTCGATTTGGTTCAGCAATTGCAGCTCTTTCAGACATCAACATGGATGGCTTTAATGATGTGATTGTTGGTTCACCACTAGAAAATCAGAATTCTGGAGCTGTATACATTTACAATGGTCATCAGGGCACTATCCGCACAAAGTATTCCCAGAAAATCTTGGGATCCGATGGAGCCTTTAGGAGCCATCTCCAGTACTTTGGGAGGTCCTTGGATGGCTATGGAGATTTAAATGGGGATTCCATCACCGATGTGTCTATTGGTGCCTTTGGACAAGTGGTTCAACTCTGGTCACAAAGTATTGCTGATGTAGCTATAGAAGCTTCATTCACACCAGAAAAAATCACTTTGGTCAACAAGAATGCTCAGATAATTCTCAAACTCTGCTTCAGTGCAAAGTTCAGACCTACTAAGCAAAACAATCAAGTGGCCATTGTATATAACATCACACTTGATGCAGATGGATTTTCATCCAGAGTAACCTCCAGGGGGTTATTTAAAGAAAACAATGAAAGGTGCCTGCAGAAGAATATGGTAGTAAATCAAGCACAGAGTTGCCCCGAGCACATCATTTATATACAGGAGCCCTCTGATGTTGTCAACTCTTTGGATTTGCGTGTGGACATCAGTCTGGAAAACCCTGGCACTAGCCCTGCCCTTGAAGCCTATTCTGAGACTGCCAAGGTCTTCAGTATTCCTTTCCACAAAGACTGTGGTGAGGACGGACAGCAACCAAAACAAAAGGTTAACATTTTCAGTAACGCTGAAAAATAAAAGGGAAAGTGCATACAACAGAAGTAACATGCCAGGTGGCTGCATCTCAGAAGTCTGTTGCCTGCGATGTAGGCTACCCTGCTTTAAAGAGAGAACAACAGGTGACTTTTACTATTAACTTTGACTTCAATCTTCAAAACCTTCAGAATCAGGCGTATTCCTCTCCTGTATGATGCTGAAATTCACTTAACAAAGGTAACAACAGGAAGTGTTCCAGTAAGCATTGCAAACAGACAAGGCTGGTGACATCAGTTGTAATGCAGATATCAATCCACTGAAAATAGGACAAACATCTTCTTCTGTATCTTTCAAAAGTGAAAATTTCAGGCACACCAAAGAATTGAACTGCAGAACTGCTTCCTGTAGTAATGTTACCTGCTGGTTGAAAGACGTTCACATGAAAGGAGAATACTTTGTTAATGTGACTACCAGAATTTGGAACGGGACTTTCGCATCATCAACGTTCCAGACAGTACAGCTAACGGCAGCTGCAGAAATCAACACCTATAACCCTGAGATATATGTGATTGAAGATAACACTGTTACGATTCCCCTGATGATAATGAAACCTGATGAGAAAGCCGAAGTACCAACAGGAGTTATAATAGGAAGTATAATTGCTGGAATCCTTTTGCTGTTAGCTCTGGTTGCAATTTTATGGAAGCTCGGCTTCTTCAAAAGAAAATATGAAAAGATGACCAAAAATCCAGATGAGATTGATGAGACCACAGAGCTCAGTAGCTGAACCAGCAGACCTACCTGNOV2a, CG191745-01SEQ ID NO: 41147 aaMW at 125495.9kDProtein SequenceMGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASFSPATQPCPSLIDVVVVCDESNSIYPWDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVIDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQIFSIEGTVQGGDNFQMEMSQVGFSADYSSQNDILMLGAVGAFGWSGTIVQKTSHGHLIFPKQAFDQILQDRNHSSYLGYSVAAISTGESTHFVAGAPRANYTGQIVLYSVNENGNITVIQAHRGDQIGSYFGSVLCSVDVDKDTITDVLLVGAPMYMSDLKKEEGRVYLFTIKEGILGQHQFLEGPEGIENTRFGSAIAALSDINMDGFNDVIVGSPLENQNSGAVYIYNGHQGTIRTKYSQKILGSDGAFRSHLQYFGRSLDGYGDLNGDSITDVSIGAFGQVVQLWSQSIADVAIEASFTPEKITLVNKNAQIILKLCFSAKFRPTKQNNQVAIVYNITLDADGFSSRVTSRGLFKENNERCLQKNMVVNQAQSCPEHIIYIQEPSDVVNSLDLRVDISLENPGTSPALEAYSETAKVFSIPFHKDCGEDGLCISDLVLDVRQIPAAQEQPFIVSNQNKRLTFSVTLKNKRESAYNTGIVVDFSENLFFASFSLPVDGTEVTCQVAASQKSVACDVGYPALKREQQVTFTINFDFNLQNLQNQASLSFQALSESQEENKADNLVNLKIPLLYDAEIHLTKVTTGSVPVSMATVIIHIPQYTKEKNPLMYLTGVQTDKAGDISCNADINPLKIGQTSSSVSEKSENFRHTKELNCRTASCSNVTCWLKDVHMKGEYFVNVTTRIWNGTFASSTFQTVQLTAAAEINTYNPEIYVIEDNTVTIPLMIMKPDEKAEVPTGVIIGSIIAGILLLLALVAILWKLGFFKRKYEKMTKNPDEIDETTELSS


Further analysis of the NOV2a protein yielded the following properties shown in Table 2B.

TABLE 2BProtein Sequence Properties NOV2aSignalP analysis:Cleavage site between residues 30 and 31PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 5; pos. chg 1; neg. chg 1H-region:length 30; peak value 9.82PSG score:5.42GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 1.12possible cleavage site: between 22 and 23>>> Seems to have a cleavable signal peptide (1 to 22)ALOM:Klein et al's method for TM region allocationInit position for calculation: 23Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 1100-1116−13.27PERIPHERALLikelihood = 0.95 (at 943)ALOM score: −13.27 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 11Charge difference: −2.0 C(−1.0)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 1117 to 1147)MITDISC:discrimination of mitochondrial targeting seqR content: 1Hyd Moment(75):10.10Hyd Moment(95): 5.95G content:4D/E content: 2S/T content:2Score: −6.90Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 15 ERT|GANUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 7.7%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: GPERnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: 1128Dileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):55.6%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: extracellular, including cell wall>> prediction for CG191745-01 is end (k = 9)


A search of the NOV2a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 2C.

TABLE 2CGeneseq Results for NOV2aNOV2aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAG79775Alpha2 integrin - Homo sapiens, 11811 . . . 11471146/1181 (97%)0.0aa. [WO2002101070-A2, 19-DEC-1 . . . 11811147/1181 (97%)2002]ABU03548Angiogenesis-associated human1 . . . 11471146/1181 (97%)0.0protein sequence #93 - Homo1 . . . 11811147/1181 (97%)sapiens, 1181 aa. [WO200279492-A2,10-OCT-2002]ABU03617Human expressed protein tag (EPT)1 . . . 11471146/1181 (97%)0.0#283 - Homo sapiens, 1181 aa.1 . . . 11811147/1181 (97%)[WO200278524-A2, 10-OCT-2002]ABU03616Human expressed protein tag (EPT)1 . . . 11471146/1181 (97%)0.0#282 - Homo sapiens, 1181 aa.1 . . . 11811147/1181 (97%)[WO200278524-A2, 10-OCT-2002]ABU03614Human expressed protein tag (EPT)1 . . . 11471146/1181 (97%)0.0#280 - Homo sapiens, 1181 aa.1 . . . 11811147/1181 (97%)[WO200278524-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV2a protein was found to have homology to the proteins shown in the BLASTP data in Table 2D.

TABLE 2DPublic BLASTP Results for NOV2aNOV2aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueP17301Integrin alpha-2 precursor (Platelet1 . . . 11471146/1181(97%)0.0membrane glycoprotein la) (GPla)1 . . . 11811147/1181(97%)(Collagen receptor) (VLA-2 alphachain) (CD49b) - Homo sapiens(Human), 1181 aa.P53710Integrin alpha-2 precursor (Platelet12 . . . 1147 986/1170(84%)0.0membrane glycoprotein la) (GPla)1 . . . 11701069/1170(91%)(Collagen receptor) (VLA-2 alphachain) (CD49b) - Bos taurus (Bovine),1170 aa (fragment).Q62469Integrin alpha-2 precursor (Platelet1 . . . 1147945/1181(80%)0.0membrane glycoprotein la) (GPla)1 . . . 11781040/1181(88%)(Collagen receptor) (VLA-2 alphachain) (CD49b) - Mus musculus(Mouse), 1178 aa.P18614Integrin alpha-1 precursor (Laminin1 . . . 1131471/1202(39%)0.0and collagen receptor) (VLA-1)1 . . . 1176678/1202(56%)(CD49a) - Rattus norvegicus (Rat),1180 aa.O42094ALPHA1 integrin - Gallus gallus29 . . . 1131 456/1179(38%)0.0(Chicken), 1171 aa.17 . . . 1167 671/1179(56%)


PFam analysis predicts that the NOV2a protein contains the domains shown in the Table 2E.

TABLE 2EDomain Analysis of NOV2aIdentities/PfamNOV2aSimilarities forExpectDomainMatch Regionthe Matched RegionValuevwa174 . . . 36164/203(32%)1.1e−47146/203(72%)FG-GAP491 . . . 55418/64(28%)0.0001252/64(81%)FG-GAP555 . . . 60214/52(27%)0.0006237/52(71%)FG-GAP618 . . . 66114/51(27%)0.07332/51(63%)integrin_A1121 . . . 11358/15(53%)0.004814/15(93%)


Example 3

The NOV3 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 3A.

TABLE 3ANOV3 Sequence AnalysisNOV3a, CG50253-01SEQ ID NO: 51882 bpDNA SequenceORF Start: ATG at 243ORF Stop: TAA at 1851GGACACTGACATGGACTGAAGGAGTAGAAAAGAAGGGAGCGGGAGGGGGCTCCGGGCGCCGCGCAGCAGACCTACTCCGGCCGCGCGCCTCGCCGCTGTCCTCCGGGAGCGGCAGCAGTAGCCCGGGCGGCGAGGGCTGGGGGTTCCTCGAGACTCTCAGAGGGGCGCCTCCCATCGGCGCCCACCACCCCAACCTGTTCCTCGCGCGCCACTGCGCTGCGCCCCAGGACCCGCTGCCCAACATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACATACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCTAACAACTCCAGAACTAACAATGAACTCCTAANOV3a, CG50253-01SEQ ID NO: 6536 aaMW at 58650.8kDProtein SequenceMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNIRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERNOV3b, 250095765SEQ ID NO: 71765 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTAATAGCCAGGATAAGGTGCCAGCTCAAAGCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGGCAACCTTCAAATGACTTGTTTGAAATATTTGAAATAGAAAGAGGAGTCAGTGCAGACGATGAAGCAAAGGATGATCCAGGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCGTCGACGGCNOV3b, 250095765SEQ ID NO: 81588 aaMW at 64525.3kDProtein SequenceTGSMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPFYVLRQRIARIRCQLKAVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFIPRQPSNDLFEIFEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3c, 250095742SEQ ID NO: 91627 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCACCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCGTCGACGGCNOV3c, 250095742SEQ ID NO: 10542 aaMW at 59162.3kDProtein SequenceTGSMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLHIAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYTCKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNov3d, 250095779SEQ ID NO: 111765 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTTTCTACGTCTTAAGGCAGAGAATAGCCAGGATAAGGTGCCAGCTCAAAGCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCACCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGGCAACCTTCAAATGACTTGTTTGAAATATTTGAAATAGAAAGAGGAGTCAGTGCAGACGATGAAGCAAAGGATGATCCAGGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATAATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAAACGCGTCGACGGCNOV3d, 250095779SEQ ID NO: 12588 aaMW at 64534.3kDProtein SequenceTGSMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPFYVLRQRIARIRCQLKAVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLHLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFIPRQPSNDLFEIFEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3e, 250095794SEQ ID NO: 131657 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCACCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGGCAACCTTCAAATGACTTGTTTGAAATATTTGAAATAGAAAGAGGAGTCAGTGCAGACGATGAAGCAAAGGATGATCCAGGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCGTCGACGGCNOV3e, 250095794SEQ ID NO: 14552 aaMW at 60397.3kDProtein SequenceTGSEFDGRWPRQTVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLHLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPITTNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFIPRQPSNDLFEIFEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3f, 250095734SEQ ID NO: 151717 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGTAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTAACCATCCCCTGGCCTGCACCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCATTCCACGGCAACCTTCAAATGACTTGTTTGAAATATTTGAAATAGAAAGAGGAGTCAGTGCAGACGATGAAGCAAAGGATGATCCAGGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAIAAGAACGCGTCGACGGCNOV3f, 250095734SEQ ID NO: 16572 aaMW at 62504.8kDProtein SequenceTGSMDFLLALVLVSSLYLQAAAEFDGSRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLHLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTTLKGDTGNNNWIPDVGSTWWPPKTPYTPPIITNRPTSKPTTRPTPKPTPTPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFIPRQPSNDLFEIFEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLNWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRBKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3g, 250095811SEQ ID NO: 17613 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTCGACGGCNov3g, 250095811SEQ ID NO: 18204 aaMW at 22351 .4kDProtein SequenceTGSCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVDGNOV3h, 250095799SEQ ID NO: 19613 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCACCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTCGACGGCNOV3h, 250095799SEQ ID NO: 20204 aaMW at 22360.4kDProtein SequenceGSCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDWKGQIRCQCPSPGLHLAPDGRTCVDVDECATGRASCPRFRQCVNFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVDGNOV3i, SNP13377609 ofSEQ ID NO: 211882bpCG50253-01, DNA SequenceORF Start: ATG at 243ORF Stop: TAA at 1851SNP Pos: 63SNP Change: G to AGGACACTGACATGGACTGAAGGAGTAGAAAAGAAGGGAGCGGGAGGGGGCTCCGGGCGCCGCACAGCAGACCTACTCCGGCCGCGCGCCTCGCCGCTGTCCTCCGGGAGCGGCAGCAGTAGCCCGGGCGGCGAGGGCTGGGGGTTCCTCGAGACTCTCAGAGGGGCGCCTCCCATCGGCGCCCACCACCCCAACCTGTTCCTCGCGCGCCACTGCGCTGCGCCCCAGGACCCGCTGCCCAACATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACATACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCTAACAACTCCAGAACTAACAATGAACTCCTAANOV3i, SNP13377609 ofSEQ ID NO: 22536 aaMW at 58650.8kDCG50253-01, Protein SequenceSNP Change: no changeMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQTAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNIRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGIAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERNOV3j, SNP13373929 ofSEQ ID NO: 231882 bpCG50253-O1, DNA SequenceORF Start: ATG at 243ORE Stop: TM at 1851SNP Pos: 226SNP Change: A to GGGACACTGACATGGACTGAAGGAGTAGAAAAGAAGGGAGCGGGAGGGGGCTCCGGGCGCCGCGCAGCAGACCTACTCCGGCCGCGCGCCTCGCCGCTGTCCTCCGGGAGCGGCAGCAGTAGCCCGGGCGGCGAGGGCTGGGGGTTCCTCGAGACTCTCAGAGGGGCGCCTCCCATCGGCGCCCACCACCCCAACCTGTTCCTCGCGCGCCACTGCGCTGCGCCCCGGGACCCGCTGCCCAACATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCAACAGTCTTAAGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACATACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCTAACAACTCCAGAACTAACAATGAACTCCTAANoV3j, SNP13373929 ofSEQ ID NO: 24536 aa1MW at 58650.8kDCG50253-01, Protein SequenceSNP Change: no changeMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNIRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERNOV3k, SNP13380272 ofSEQ ID NO: 251882 bpCG50253-01, DNA SequenceORF Start: ATG at 243ORF Stop: TM at 1851SNP Pos: 719SNP Change: G to CGGACACTGACATGGACTGAAGGAGTAGAAAAGAAGGGAGCGGGAGGGGGCTCCGGGCGCCGCGCAGCAGACCTACTCCGGCCGCGCGCCTCGCCGCTGTCCTCCGGGAGCGGCAGCAGTAGCCCGGGCGGCGAGGGCTGGGGGTTCCTCGAGACTCTCAGAGGGGCGCCTCCCATCGGCGCCCACCACCCCAACCTGTTCCTCGCGCGCCACTGCGCTGCGCCCCAGGACCCGCTGCCCAACATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCACCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACATACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGAACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCTAACAACTCCAGAACTAACAATGAACTCCTAANOV3k, SNP13380272 ofSEQ ID NO: 26536 aaMW at 58659.8kDCG50253-01, Protein SequenceSNP Pos: 159SNP Change: Gln to HisMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLHLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNIRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERNov3l, SNP13379745 ofSEQ ID NO: 271882 bpCG50253-01, DNA SequenceORF Start: ATG at 243ORF Stop: TAA at 1851SNP Pos: 750SNP Change: G to AGGACACTGACATGGACTGAAGGAGTAGAAAAGAAGGGAGCGGGAGGGGGCTCCGGGCGCCGCGCAGCAGACCTACTCCGGCCGCGCGCCTCGCCGCTGTCCTCCGGGAGCGGCAGCAGTAGCCCGGGCGGCGAGGCCTGGGGGTTCCTCGAGACTCTCAGAGGGGCGCCTCCCATCGGCGCCCACCACCCCAACCTGTTCCTCGCGCGCCACTGCGCTGCGCCCCAGGACCCGCTGCCCAACATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATATTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACATACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCTAACAACTCCAGAACTAACAATGAACTCCTAANOV3l, SNP13379745 ofSEQ ID NO: 28536 aaMW at 58664.8kDCG50253-01, Protein SequenceSNP Pos: 170SNP Change: Val to IleMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQLAPDGRTCVDIDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNIRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLNWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERNOV3m, SNP13373930 ofSEQ ID NO: 291882 bpCG50253-01, DNA SequenceORF Start: ATG at 243ORF Stop: TAA at 1851SNP Pos: 942SNP Change: A to GGGACACTGACATGGACTGAAGGAGTAGAAAAGAAGGGAGCGGGAGGGGGCTCCGGGCGCCGCGCAGCAGACCTACTCCGGCCGCGCGCCTCGCCGCTGTCCTCCGGGAGCGGCAGCAGTAGCCCGGGCGGCGAGGGCTGGGGGTTCCTCGAGACTCTCAGAGGGGCGCCTCCCATCGGCGCCCACCACCCCAACCTGTTCCTCGCGCGCCACTGCGCTGCGCCCCAGGACCCGCTGCCCAACATGGATTTTCTCCTGGCGCTGGTGCTGGTATCCTCGCTCTACCTGCAGGCGGCCGCCGAGTTCGACGGGAGGTGGCCCAGGCAAATAGTGTCATCGATTGGCCTATGTCGTTATGGTGGGAGGATTGACTGCTGCTGGGGCTGGGCTCGCCAGTCTTGGGGACAGTGTCAGCCTGTGTGCCAACCACGATGCAAACATGGTGAATGTATCGGGCCAAACAAGTGCAAGTGTCATCCTGGTTATGCTGGAAAAACCTGTAATCAAGATCTAAATGAGTGTGGCCTGAAGCCCCGGCCCTGTAAGCACAGGTGCATGAACACTTACGGCAGCTACAAGTGCTACTGTCTCAACGGATATATGCTCATGCCGGATGGTTCCTGCTCAAGTGCCCTGACCTGCTCCATGGCAAACTGTCAGTATGGCTGTGATGTTGTTAAAGGACAAATACGGTGCCAGTGCCCATCCCCTGGCCTGCAGCTGGCTCCTGATGGGAGGACCTGTGTAGATGTTGATGAATGTGCTACAGGAAGAGCCTCCTGCCCTAGATTTAGGCAATGTGTCAACACTTTTGGGAGCTACATCTGCAAGTGTCATAAAGGCTTCGATCTCATGTATATTGGAGGCAAATATCAATGTCATGACATAGACGAATGCTCACTTGGTCAGTATCAGTGCAGCAGCTTTGCTCGATGTTATAACGTACGTGGGTCCTACAAGTGCAAATGTAAAGAAGGATACCAGGGTGATGGACTGACTTGTGTGTATATCCCAAAAGTTATGATTGAACCTTCAGGTCCAATTCATGTACCAAAGGGAAATGGTACCATTTTAAAGGGTGACACAGGAAATAATAATTGGATTCCTGATGTTGGAAGTACTTGGTGGCCTCCGAAGACACCATATATTCCTCCTATCATTACCAACAGGCCTACTTCTAAGCCAACAACAAGACCTACACCAAAGCCAACACCAATTCCTACTCCACCACCACCACCACCCCTGCCAACAGAGCTCAGAACACCTCTACCACCTACAACCCCAGAAAGGCCAACCACCGGACTGACAACTATAGCACCAGCTGCCAGTACACCTCCAGGAGGGATTACAGTTGACAACAGGGTACAGACAGACCCTCAGAAACCCAGAGGAGATGTGTTCAGTGTTCTGGTACACAGTTGTAATTTTGACCATGGACTTTGTGGATGGATCAGGGAGAAAGACAATGACTTGCACTGGGAACCAATCAGGGACCCAGCAGGTGGACAATATCTGACAGTGTCGGCAGCCAAAGCCCCAGGGGGAAAAGCTGCACGCTTGGTGCTACCTCTCGGCCGCCTCATGCATTCAGGGGACCTGTGCCTGTCATTCAGGCACAAGGTGACGGGGCTGCACTCTGGCACACTCCAGGTGTTTGTGAGAAAACACGGTGCCCACGGAGCAGCCCTGTGGGGAAGAAATGGTGGCCATGGCTGGAGGCAAACACAGATCACCTTGCGAGGGGCTGACATCAAGAGCGTCGTCTTCAAAGGTGAAAAAAGGCGTGGTCACACTGGGGAGATTGGATTAGATGATGTGAGCTTGAAAAAAGGCCACTGCTCTGAAGAACGCTAACAACTCCAGAACTAACAATGAACTCCTAANOV3m, SNP13373930 ofSEQ ID NO: 30536 aaMW at 58636.8kDCG50253-01, Protein SequenceSNP Pos: 234SNP Change: Ile to ValMDFLLALVLVSSLYLQAAAEFDGRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCQPVCQPRCKHGE+TL,42CIGPNKCKCHPGYAGKTCNQDLNECGLKPRPCKHRCMNTYGSYKCYCLNGYMLMPDGSCSSALTCSMANCQYGCDVVKGQIRCQCPSPGLQLAPDGRTCVDVDECATGRASCPRFRQCVNTFGSYICKCHKGFDLMYIGGKYQCHDIDECSLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVYIPKVMIEPSGPIHVPKGNGTILKGDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFSVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEER


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 3B.

TABLE 3BComparison of the NOV3 protein sequences.NOV3a---MDFLLALVLVSSLYLQAAAEFDG-RWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCNOV3bTGSMDFLLALVLVSSLYLQAAAEFDG-RWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCNOV3cTGSMDFLLALVLVSSLYLQAAAEFDG-RWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCNOV3dTGSMDFLLALVLVSSLYLQAAAEFDG-RWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCNOV3e-------------------TGSEFDG-RWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCNOV3fTGSMDFLLALVLVSSLYLQAAAEFDGSRWPRQIVSSIGLCRYGGRIDCCWGWARQSWGQCNOV3g--------------------------------------------------------TGSCNOV3h--------------------------------------------------------TGSCNOV3aQP-----------------VCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPNOV3bQPFYVLRQRTARIRCQLKAVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPNOV3cQP-----------------VCQPRCKHGECTGPNKCKCHPGYAGKTCNQDLNECGLKPRPNOV3dQPFYVLRQRIAPTRCQLKAVCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPNOV3eQP-----------------VCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPNOV3fQP-----------------VCQPRCKHGECIGPNXCKCHPGYAGKTCNQDLNECGLKPRPNOV3gQP-----------------VCQPRCKHGECIGPNKCKCHPGYAGKTCNQDLNECGLKPRPNOV3hQP-----------------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-----------------------NOV3hLGQYQCSSFARCYNVRGSYKCKCKEGYQGDGLTCVDG-----------------------NOV3aDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRNOV3bDTGNNNWIPDVGSTWWPPKTPYIPPTITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRNOV3cDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRNOV3dDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRNOV3eDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRNOV3fDTGNNNWIPDVGSTWWPPKTPYIPPIITNRPTSKPTTRPTPKPTPIPTPPPPPPLPTELRNOV3g------------------------------------------------------------NOV3h------------------------------------------------------------NOV3aTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFS------------NOV3bTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFTPRQPSNDLFEIFNOV3cTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFS------------NOV3dTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFTPRQPSNDLFEIFNOV3eTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFTPRQPSNDLFEIFNOV3fTPLPPTTPERPTTGLTTIAPAASTPPGGITVDNRVQTDPQKPRGDVFTPRQPSNDLFEIFNOV3g------------------------------------------------------------NOV3h------------------------------------------------------------NOV3a-----------------VLVHSCNFDHGLCGWIREKDNDLHWEPIPDPAGGQYLTVSAAKNOV3bEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIPDPAGGQYLTVSAAKNOV3c-----------------VLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKNOV3dEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKNOV3eEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKNOV3fEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKNOV3g------------------------------------------------------------NOV3h------------------------------------------------------------NOV3aAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGNOV3bAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGNOV3cAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGNOV3dAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGNOV3eAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGNOV3fAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGNOV3g------------------------------------------------------------NOV3h------------------------------------------------------------NOV3aWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEER---NOV3bWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3cWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3dWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3eWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3fWRQTQITLRGADIKSVVFKGEKRRGHTGEIGLDDVSLKKGHCSEERVDGNOV3g-------------------------------------------------NOV3h-------------------------------------------------NOV3a(SEQ ID NO: 6)NOV3b(SEQ ID NO: 8)NOV3c(SEQ ID NO: 10)NOV3d(SEQ ID NO: 12)NOV3e(SEQ ID NO: 14)NOV3f(SEQ ID NO: 16)NOV3g(SEQ ID NO: 18)NOV3h(SEQ ID NO: 20)


Further analysis of the NOV3a protein yielded the following properties shown in Table 3C.

TABLE 3CProtein Sequence Properties NOV3aSignalP analysis:Cleavage site between residues 20 and 21PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 2; pos. chg 0; neg. chg 1H-region:length 17; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.34possible cleavage site: between 17 and 18>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 3-19−4.19PERIPHERALLikelihood = 5.67 (at 439)ALOM score: −4.19 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 10Charge difference: 0.0 C(0.0)-N(0.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 3)MITDISC:discrimination of mitochondrial targeting seqR content: 0Hyd Moment(75):4.41Hyd Moment(95): 7.23G content:0D/E content: 2S/T content:2Score: −6.55Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 11.9%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):43.5%: nuclear26.1%: mitochondrial 8.7%: cytoplasmic 4.3%: plasma membrane 4.3%: vesicles of secretory system 4.3%: extracellular, including cell wall 4.3%: endoplasmic reticulum 4.3%: peroxisomal>> prediction for CG50253-01 is nuc (k = 23)


A search of the NOV3a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 3D.

TABLE 3DGeneseq Results for NOV3aNOV3aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG70290Human novel polypeptide #6 - Homo1 . . . 536 536/536 (100%)0.0sapiens, 536 aa. [WO200257452-A2,1 . . . 536 536/536 (100%)25-JUL-2002]ABG69659Human secreted protein SCEP-39 -1 . . . 536534/566 (94%)0.0Homo sapiens, 566 aa.1 . . . 566535/566 (94%)[WO200248337-A2, 20-JUN-2002]AAB70547Human PRO17 protein sequence SEQ1 . . . 536532/582 (91%)0.0ID NO: 34 - Homo sapiens, 582 aa.1 . . . 582533/582 (91%)[WO200110902-A2, 15-FEB-2001]AAB70549Clone 16467945.0.85-S261.D protein20 . . . 536 512/546 (93%)0.0sequence SEQ ID NO: 82 - Homo1 . . . 546514/546 (93%)sapiens, 546 aa. [WO200110902-A2,15-FEB-2001]ABU58425Human PRO polypeptide #26 - Homo1 . . . 505503/505 (99%)0.0sapiens, 509 aa. [US2003027272-A1,1 . . . 505504/505 (99%)06-FEB-2003]


In a BLAST search of public sequence databases, the NOV3a protein was found to have homology to the proteins shown in the BLASTP data in Table 3E.

TABLE 3EPublic BLASTP Results for NOV3aNOV3aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueCAC33425Sequence 33 from Patent1 . . . 536532/582 (91%)0.0WO0110902 - Homo sapiens1 . . . 582533/582 (91%)(Human), 582 aa.Q91V88POEM (NEPHRONECTIN short1 . . . 532470/562 (83%)0.0isoform) - Mus musculus (Mouse),1 . . . 561497/562 (87%)561 aa.Q91ZD3Nephronectin long isoform - Mus1 . . . 532470/579 (81%)0.0musculus (Mouse), 578 aa.1 . . . 578497/579 (85%)Q91XL5Nephronectin - Mus musculus1 . . . 532470/593 (79%)0.0(Mouse), 592 aa.1 . . . 592497/593 (83%)Q923T5Nephronectin - Mus musculus1 . . . 532470/610 (77%)0.0(Mouse), 609 aa.1 . . . 609497/610 (81%)


PFam analysis predicts that the NOV3a protein contains the domains shown in the Table 3F.

TABLE 3FDomain Analysis of NOV3aIdentities/Similarities forPfamNOV3athe MatchedExpectDomainMatch RegionRegionValueC_tripleX54 . . . 71 7/18(39%)0.2415/18(83%)EGF60 . . . 86 13/47(28%)0.1323/47(49%)EGF93 . . . 12715/47(32%)0.5127/47(57%)EGF133 . . . 167 10/47(21%)0.03524/47(51%)EGF218 . . . 253 12/47(26%)0.0001128/47(60%)MAM393 . . . 534 48/183(26%)8.6e−2798/183(54%)


Example 4

The NOV4 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 4A.

TABLE 4ANOV4 Sequence AnalysisNOV4a, CG50377-04SEQ ID NO: 3110655 bpDNA SequenceORF Start: at 1ORF Stop: TAG at 10639GGCCAGAACTGCACGTTCCAACTGCACGGTCCCAATGGGACAGTTGAGAGCCCAGGGTTCCCATATGGCTACCCCAATTACGCCAACTGCACGTGGACCATCACCGCGGAAGAGCAGCACAGAATCCAGCTTGTGTTCCAGTCCTTTGCCCTGGAAGAGGACTTTGATGTCCTGTCGGTGTTTGATGGTCCACCCCAGCCAGAGAATCTGCGTACGAGGCTCACAGGCTTTCAGCTGCCAGCCACCATTGTTAGTGCAGCCACCACCCTCTCTCTGCGCCTCATCAGCGACTATGCAGTCAGTGCCCAAGGCTTCCACGCCACCTATGAAGTTCTCCCCAGCCACACATGTGGGAACCCAGGGAGGCTGCCCAATGGCATCCAGCAGGGTTCAACCTTCAACCTCGGTGACAAGGTCCGCTACAGCTGCAACCTTGGCTTCTTCCTGGAGGGCCACGCCGTGCTCACCTGCCACGCTGGCTCTGAGAACAGCGCCACGTGGGACTTCCCCCTGCCTTCCTGCAGAGCTGATGATGCCTGTGGTGGGACCCTGCGGGGCCAGAGTGGCATCATCTCCAGCCCCCACTTCCCCTCGGAGTACCATAACAATGCCGACTGCACATGGACCATCCTGGCTGAGCTGGGGGACACCATCGCCCTGGTGTTTATTGACTTCCAGCTGGAGGATGGTTACGACTTTCTGGAAGTCACTGGGACAGAAGGCTCCTCCCTCTGGTTCACCGGAGCCAGCCTCCCAGCCCCCGTTATCAGCAGCAAGAACTGGCTGCGACTGCACTTCACATCGGATGGCAACCACCGGCAGCGCGGATTCAGTGCCCAATACCAAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGGTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTAATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGCTGACAGGTACATCGGTCCCGGATCTCATTGTCAGCACCAATCATCAAATGTGGCTCCTCTTCCAGACTGATGGCAGTGGCAGTTCCCTGGGATTCAAGGCTTCTTATGAAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCGCCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTGTTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCAGTGAGTCCTCCAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGGTTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGGCTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGTTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGTTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGGTTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTGTTCCCCAGCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTTCCCAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGGTGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGTGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCCTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATCTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAATGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCNOV4a, CG50377-04SEQ ID NO: 323546 aaMW at 385864.BkDProtein SequenceGQNCTFQLHGPNGTVESPGFPYGYPNYANCTWTITAEEQHRIQLVFQSFALEEDFDVLSVFDGPPQPENLRTRLTGFQLPATIVSAATTLSLRLISDYAVSAQGFHATYEVLPSHTCGNPGRLPNGIQQGSTFNLGDKVRYSCNLGFFLEGHAVLTCHAGSENSATWDFPLPSCRADDACGGTLRGQSGIISSPHFPSEYHNNADCTWTILAELGDTIALVFIDFQLEDGYDFLEVTGTEGSSLWFTGASLPAPVISSKNWLRLHFTSDGNHRQRGFSAQYQVKKQIELKSRGVKLMPSKDNSQKTSVVTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRAEMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMLTGTSVPDLIVSTNHQMWLLFQTDGSGSSLGFKASYEEIEQGSCGDPGIPAYGRREGSRFRHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVCSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTSESSNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYEAITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLLGVFSHSENMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSGSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVLFPSFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDFPENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYGVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPEAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPPICLEVRPNGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNSSSVAAAILVPFIALIIAGFVLYLYKHRRRPKVPFNGYAGHENTNVPATFENPMYDRNIQPTDIMASEAEFTVSTVCTAVNOV4b, CG50377-01SEQ ID NO: 3310136 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 9313ATGGCGGGCGCCCCTCCCCCCGCCTTGCTGCTGCCTTGCAGTTTGATCTCAGACTGCTGTGCTAGCAATCAGCGACACTCCGTGGGCGTAGGACCCTCCGAGCTAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGTTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTGATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGTCTCAAAATGCCTGCAGTGACAGCCCTCACACCCCAGGCTCTCGCATCCCAGAGAGCATGTCTGGGGACATCTGGAGGCAGAAATGGACTGTACTTGAGATCTGTCGTGACATTAGCAGTTCAGATGCAAGGTCAGGTTCAGTGAGGAAGTCTCCAAAGACTTCTAATGCTGTGGAACTTGTTGCTCCTGGGACAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCACCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTTCTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCACCTTCCGACACAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGATTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGACTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGGTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGCTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGTGTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTTAACATCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTATACAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCGTGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCTTTTTTTGCTAGGTTGAACTGGTACTCCAGCAGCCGCCGAAGCTGGACTGTACTGCTGCCATCTCAGCTCACTGCAACCTCCCTGCCTGATTCCCCTGCCTCAGCCTGCCGAGTGCCTGCGATTGCAGGCGCGCACCGCCACNov4b, CG50377-01SEQ ID NO: 343104 aaMW at 336746.3kDProtein SequenceMAGAPPPALLLPCSLISDCCASNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKAPAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNCSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVFNIFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEENEGVILSPGFPGNYPSNNDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWPLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGBVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSRVAPSTAPARRMAAGQASRPSAWRSGPVGDPSTLPGSHRSPKPNOV4c, CG50377-02SEQ ID NO: 358010 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 8008ATGGCGGGCGCCCCTCCCCCCGCCTTGCTGCTGCCTTGCAGTTTGATCTCAGACTGCTGTGCTAGCAATCAGCGACACTCCGTGGGCGTAGGACCCTCCGAGCTAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGTTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTGATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGTCTCAAAATGCCTGCAGTGACAGCCCTCACACCCCAGGCTCTCGCATCCCAGAGAGCATGTCTGGGGACATCTGGAGGCAGAAATGGACTGTACTTGAGATCTGTCGTGACATTAGCAGTTCAGATGCAAGGTCAGGTTCAGTGAGGAAGTCTCCAAAGACTTCTAATGCTGTGGAACTTGTTGCTCCTGGGACAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCACCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTTCTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCACCTTCCGACACAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGATTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGACTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGGTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGCTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGTGTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTTAACATCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTATACAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCGTGAGTCCTCGGGCAATGGAGGCGGGTCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCCACTCAGACCAAGCTCCACTCCATTTTCTATAAGCTCCTCTTCGATGTACTCTCTTCCCCATCCCTCACCAAAGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCATGTTAAGCAGCAGTTGCTGCTGCTGCTGCTGCTGTTGTGTGATGATGATGATGATGAAGATGATGGTAGTGGTGCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTGTAAGTAGNOV4c, CG50377-02SEQ ID NO: 362669 aaMW at 290805.9kDProtein SequenceMAGAPPPALLLPCSLISDCCASNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCPARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSPALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKAPAFELSEGDVLKVYDGNNNSARLLGVFSHSENMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVFNIFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGAIAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVRESSGNGGGSVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLATQTKLHSIFYKLLFDVLSSPSLTKAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVHVKQQLLLLLLLLCDDDDDEDDGSGAITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLCKNOV4d, CG50377-03SEQ ID NO: 379951 bpDNA SequenceORF Start: ATG at 1ORf Stop: TGA at 7837ATGGCGGGCGCCCCTCCCCCCGCCTTGCTGCTGCCTTGCAGTTTGATCTCAGACTGCTGTGCTAGCAATCAGCGACACTCCGTGGGCGTAGGACCCTCCGAGCTAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGTTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTGATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGTCTCAAAATGCCTGCAGTGACAGCCCTCACACCCCAGGCTCTCGCATCCCAGAGAGCATGTCTGGGGACATCTGGAGGCAGAAATGGACTGTACTTGAGATCTGTCGTGACATTAGCAGTTCAGATGCAAGGTCAGGTTCAGTGAGGAAGTCTCCAAAGACTTCTAATGCTGTGGAACTTGTTGCTCCTGGGACAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCACCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTTCTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCACCTTCCGACACAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGATTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGACTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGGTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGCTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGTGTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTTAACATCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTATACAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTGTAAGTAGTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGQGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCTTTTTTTGCTAGGTTGAACTGGTACTCCAGCAGCCGCCGAAGCTGGACTGTACTGCTGCCATCTCAGCTCACTGCAACCTCCCTGCCTGATTCCCCTGCCTCAGCCTGCCGAGTGCCTGCGATTGCAGGCGCGCACCGCCACNOV4d, CG50377-03SEQ ID NO: 382612 aaMW at 284047.5kDProtein SequenceMAGAPPPALLLPCSLISDCCASNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTTLSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVFNIFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVVLRWSSDHAYNRKGFKIRYSALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCQLPVDTQATLSTASLRVTSLTSTMWSSLFATLGIWLRGLLGPNAWPAGNGVTCCPPAESSTVQILDTKKIVFVRSTPAARTGSASAPLCLTGATTASTSWAPQCSAAREMAHGTVPAPSVSVSSGVLWPSGLPASLPDVWRQLYCGSSGAVQLHRQAYSGGKQHPHVWAGWTLDWLPPSLLPNQRGSLRNOV4e, CG50377-05SEQ ID NO: 3910466 bpDNA SequenceORF Start: at 1ORF Stop: TAG at 10450GGCCAGAACTGCACGTTCCAACTGCACGGTCCCAATGGGACAGTTGAGAGCCCAGGGTTCCCATATGGCTACCCCAATTACGCCAACTGCACGTGGACCATCACCGCGGAAGAGCAGCACAGAATCCAGCTTGTGTTCCAGTCCTTTGCCCTGGAAGAGGACTTTGATGTCCTGTCGGTGTTTGATGGTCCACCCCAGCCAGAGAATCTGCGTACGAGGCTCACAGGCTTTCAGCTGCCAGCCACCATTGTTAGTGCAGCCACCACCCTCTCTCTGCGCCTCATCAGCGACTATGCAGTCAGTGCCCAAGGCTTCCACGCCACCTATGAAGTTCTCCCCAGCCACACATGTGGGAACCCAGGGAGGCTGCCCAATGGCATCCAGCAGGGTTCAACCTTCAACCTCGGTGACAAGGTCCGCTACAGCTGCAACCTTGGCTTCTTCCTGGAGGGCCACGCCGTGCTCACCTGCCACGCTGGCTCTGAGAACAGCGCCACGTGGGACTTCCCCCTGCCTTCCTGCAGAGCTGATGATGCCTGTGGTGGGACCCTGCGGGGCCAGAGTGGCATCATCTCCAGCCCCCACTTCCCCTCGGAGTACCATAACAATGCCGACTGCACATGGACCATCCTGGCTGAGCTGGGGGACACCATCGCCCTGGTGTTTATTGACTTCCAGCTGGAGGATGGTTACGACTTTCTGGAAGTCACTGGGACAGAAGGCTCCTCCCTCTGGTTCACCGGAGCCAGCCTCCCAGCCCCCGTTATCAGCAGCAAGAACTGGCTGCGACTGCACTTCACATCGGATGGCAACCACCGGCAGCGCGGATTCAGTGCCCAATACCAAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGGTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTAATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGCTGACAGGTACATCGGTCCCGGATCTCATTGTCAGCACCAATCATCAAATGTGGCTCCTCTTCCAGACTGATGGCAGTGGCAGTTCCCTGGGATTCAAGGCTTCTTATGAAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCGCCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTGTTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCAGTGAGTCCTCCAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGGTTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGGCTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGTTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGTTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGGTTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTGTTCCCCAGCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTTCCCAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGGTGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGTGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCCTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATCTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAATGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCNOV4e, CG50377-05SEQ ID NO: 403483 aaMW at 379077.1kDProtein SequenceGQNCTFQLHGPNGTVESPGFPYGYPNYANCTWTITAEEQHRIQLVFQSFALEEDFDVLSVFDGPPQPENLRTRLTGFQLPATIVSAATTLSLRLISDYAVSAQGFHATYEVLPSHTCGNPGRLPNGIQQGSTFNLGDKVRYSCNLGFFLEGHAVLTCHAGSENSATWDFPLPSCRADDACGGTLRGQSGIISSPHFPSEYHNNADCTWTILAELGDTIALVFIDFQLEDGYDFLEVTGTEGSSLWFTGASLPAPVISSKNWLRLHFTSDGNHRQRGFSAQYQVKKQIELKSRGVKLMPSKDNSQKTSWTQVGVSQGHNMCPDPGIPERGKREJGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCEARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMLTGTSVPDLIVSTNHQMWLLFQTDGSGSSLGFKASYEEIEQGSCGDPGIPAYGRREGSRFRHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVCSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTSESSNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYEAITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSGSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVLFPSFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDFPENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYGVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPPICLEVRPNGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNSSSVAAAILVPFIALIIAGFVLYLYKHRRRPKVPFNGYAGHENTNVRATFENPMYDRNIQPTDIMASEAEFTVSTVCTAVNOV4f, CG50377-06SEQ ID NO: 4112901 bpDNA SequenceORF Start: ATG at 889ORF Stop: TAG at 10279GGCCAGAACTGCACGTTCCAACTGCACGGTCCCAATGGGACAGTTGAGAGCCCAGGGTTCCCATATGGCTACCCCAATTACGCCAACTGCACGTGGACCATCACCGCGGAAGAGCAGCACAGAATCCAGCTTGTGTTCCAGTCCTTTGCCCTGGAAGAGGACTTTGATGTCCTGTCGGTGTTTGATGGTCCACCCCAGCCAGAGAATCTGCGTACGAGGCTCACAGGCTTTCAGCTGCCAGCCACCATTGTTAGTGCAGCCACCACCCTCTCTCTGCGCCTCATCAGCGACTATGCAGTCAGTGCCCAAGGCTTCCACGCCACCTATGAAGTTCTCCCCAGCCACACATGTGGGAACCCAGGGAGGCTGCCCAATGGCATCCAGCAGGGTTCAACCTTCAACCTCGGTGACAAGGTCCGCTACAGCTGCAACCTTGGCTTCTTCCTGGAGGGCCACGCCGTGCTCACCTGCCACGCTGGCTCTGAGAACAGCGCCACGTGGGACTTCCCCCTGCCTTCCTGCAGAGCTGATGATGCCTGTGGTGGGACCCTGCGGGGCCAGAGTGGCATCATCTCCAGCCCCCACTTCCCCTCGGAGTACCATAACAATGCCGACTGCACATGGACCATCCTGGCTGAGCTGGGGGACACCATCGCCCTGGTGTTTATTGACTTCCAGCTGGAGGATGGTTACGACTTTCTGGAAGTCACTGGGACAGAAGGCTCCTCCCTCTGGTTCACCGGAGCCAGCCTCCCAGCCCCCGTTATCAGCAGCAAGAACTGGCTGCGACTGCACTTCACATCGGATGGCAACCACCGGCAGCGCGGATTCAGTGCCCAATACCAAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGGTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTAATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGCTGACAGGTACATCGGTCCCGGATCTCATTGTCAGCACCAATCATCAAATGTGGCTCCTCTTCCAGACTGATGGCAGTGGCAGTTCCCTGGGATTCAAGGCTTCTTATGAAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCGCCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTGTTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCAGTGAGTCCTCCAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGGTTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGGCTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGTTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCAATTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGTTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGGTTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTGTTCCCCAGCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTTCCCAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGGTGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGTGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATCTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAATGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCCACCACCGCCACCGAGAGCCCCGCCTCCAGCAGCCGTTCCCCAGGGGGCAGGAAGTAGGCTCCCCTGAGCACAGTGCCCAGTGCCCATCTTCGTTCGTCTCCTTGCCTGTGTCTGCTACTGCTGGGACTCAGGCCCTCGCCCTGCCGCCATCTATGCCAGTTACCCTGGAGAGGACTCCTGCCGTGTCCTGAAAAGGCAGAAAAGGAAGAGGGGCTGCAGACAGAGGAAGAAAGTTTATGGATTTAAAATAGCAAAGCAACTTGTGGATCTGTGTGTGTGTGTGTGTGTGTGTGTATGGGCCTGATCACACACAAACACACACACCTCTCCTTCATTCACGTCCTATGGAAGAGACCATAAAGGCTTCAAAACTAGAAGTGCGCATGAAGGATGCTTCATGCCCTGTTCCCCACACAGAGAAGAGATGAAGAGGGGGAGGTACGGTCAGCTTTCAAAACAATATGCCCCCCGCCCATCCCATGTGTGAGGACATGGTGACTCGGTGACTGTAACAGTAGGACACATCCCATCATGCGGTCAGAGTGCAGAAACCCGGTCAGCGTGCAGAAACCAGCTGTCTGCTGATGAACGGCCCATCTGCACAGCGGCAGCAAGGATTACCCCAAGGGCAGGAAAAAAGTCTTTGGAPATAGATTGAAAATCAGTTTCTTTATTTTTTTCAAACACTGTTTTATTGAGTTCTAAACCCAGTGGATTAAAAAGTCATTTGGGTCCTGGGAAGCACTGGGAAAGAAGGGAGAGCCGGGGAGGATAGGGCAGGAGTCTGGCCAGGAGCCCAAGGTCAAGTCTGGCTGTCAACTGACAAAGAGAAGAGAAGACAAGTCTGTGGCCCAGACAGGGTGCCAAGTCTGGGGGCAGCAGCAGGGTCCTTGAGAGAACAGGGGAGCAAGCCTGCCCCAGTACCCAAAAAACAATGGCTATCATGTACTGGGGTTATTCTCTGTGCCTGACACTTTACCTATAGTATTTCTAGTCTGCACAATAACACCAAAATGTAGGATTTGTTATTCCCCTTTGACAGATGAGAAAACAGAAGCCTGCAAAGGTAAAGACAGAGTTAGGAAGTGTGGAGTCAGAATGCAAACCCAGATCAGTCTGCCCCAAAGTCCACTACACCAGCTGCCAGAAACCCTGTTCTAGAAGGGGCAGCTAAGAAGGGAGGAGCCGATGAAACTCTACAGTAGCACTGTGGCCAGGTCTGGAAAGCCAGGCAAGACACCTGTGTGTCCTATCTGGGTTCTGCTGTCTCCGCGTAGGCAGCTTCGGGGCAAGGATTCAACTCCCCAGGCCTCCCACGGTTGGTGCTGGCTCCTTGGCTCCCAGCAGCCGCTGACCATAGCCAGCAAAAGACCTGGCAGGTCCTGAATCAAAACAACAGAGTGGCTGAAAGTCACACCTCTCCAGCTCCTTGCGAGTGGGTTTGATTGATTTTGACAGCTATATCATGCTTGGGTCGTTCATGAATTCCATGAGAATTGTTGGGAAGTTCATGAGTTTAGGAATCAAAGTATAGCATTTAGGGCCACTTTCTCTGTCCTCGGGAAGGCCCCCACAAGCTTAAGCCCCTGTATACGTGTGACATGCTGGCCTCAGAGATTCATGTCACATATTGAAATAGCAAATAGAAATGTGCAGGAGCGGAGGGACAGCGACACCTGGTTCTTAAGCAAATTCTAGATTGGAACAATTAGAAGATCTGACTGATGTTAAGAGTGGCCAGAGGAAAGTGAGAGGCGTCCATTTCCAGCAGACACAATGGAGGCTGACAGAGGCAGGAGCCTACCCCTGGCCACACCACCTCTGGGACACGGCACCCCCCTGGGCCTGGAGGGAGAGGGCCAATCCAGACACACTCACACTCTCATCTCTTTCCTTGGCTGGAAACAAGTGCAGGAAGCTCCCCTCCCAATTTCTTATGCTCCCTTCCCCCTTACACTCCTCTCCTTCGACTGCTCCCTTCCCCCTTCTTATGCCCCTCTCAGACCACCCAGCGGATGCCTATTCCCGCCCCCCACCCCTCCCCTGCGTCTCCTTGTAAAGTTCCATGAGATGCTCGAGGAGCACCTGCTGGTGGGGCACCCAGCACTCTGCTGGGCATCACCCTCCGCACCCCTGACATTTCTCTCCACCTCCAGAGGCTTGGAAGGGAACAGGGCAGATGAGGCCCTTCACCCACCATCTACAACATATACCCATCACCATCCCATGAGGAAAAAAAAAAAAGTAAAACAAAGACAAAAAAAATCTTTTGTACAGAGATATATTTTTATTATACAAAGTTTGTACAGTACCAAAAAAGAAAAAAAAAAGGTGTAAATTTTTTTTTCATTATTGTAGGTAAACGGTAGTGGAAGCCTTTTTTGTAAATGTAATAAAATGTATAAATATGGTTTTCAGAAAACATCAAGTGCTGTAAATATGTAATCTACACACCTTCTTGGCTTGTCTGCAGTATATACATTTAATTTATCTGTCTCTGTATATGAGGCTTCCCCCTGGGGTCCTGCATTATGGTACTTTCCTGAATTTGAAAGCAATTTTAAATTTTTTGAATTCAAATAAAATATAAATTTTTGCATTGNOV4f, CG50377-06SEQ ID NO: 423130 aaMW at 340344.5kDProtein SequenceMPSKDNSQKTSVVTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCPARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMLTGTSVPDLIVSTNHQMWLLFQTDGSGSSLGFKASYEEIEQGSCGDPGIPAYGRREGSRFRHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVCSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTSESSNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVPDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYEAITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKAEAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSGSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVLFPSFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDFPENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYGVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHAINGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPPICLEVRPNGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNSSSVAAAILVPFIALIIAGFVLYLYKHRRRPKVPFNGYAGHENTNVRATFENPMYDRNIQPTDIMASEAEFTVSTVCTAVNOV4g, 273095147SEQ ID NO: 432329 bpDNA SequenceORF Start: at 1ORF Stop: at 2329AAGCTTGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTQGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACTCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTTACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACCCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAGTGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTGTAAGGTCGACCNOV4g, 273095147SEQ ID NO: 44776 aaMW at 84741.1kDProtein SequenceKLECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHSLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGGRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLCKVDNov4h, 317459653SEQ ID NO: 452328 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAGCTTGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCGTGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTGTAAGGTCGACNOV4h, 317459653SEQ ID NO: 46776 aaMW at 84719.1kDProtein SequenceKLECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLCKVDNOV4i, 317459612SEQ ID NO: 472139 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAGCTTGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCATGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCCTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTGTAAGGTCGACNOV4i, 317459612SEQ ID NO: 48713 aaMW at 77947.4kDProtein SequenceKLECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFTTIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRPIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLCKVDNOV4j, 317286331SEQ ID NO: 492607 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAGCTTTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATGTCGACNOVv4j, 317286331SEQ ID NO: 50869 aaMW at 94553.5kDProtein SequenceKLSCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYVDNOV4k, CG50377-07SEQ ID NO: 51300 bpDNA SequenceORF Start: at 2ORF Stop: at 299AAAGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTGCCCCANOV4k, CG50377-07SEQ ID NO: 5299 aaMW at 10565.8kDProtein SequenceKSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQSTTRTCLPNLTWSGTPPDCVPNOV4l, CG50377-08SEQ ID NO: 531792 bpDNA SequenceORF Start: at 1ORF Stop: at 1792GCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAGTGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATCTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACATTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGNOV4l, CG50377-08SEQ ID NO: 54597 aaMW at 63914.5kDProtein SequenceAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALNCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPPICLEVRPSGRPINIAREPPLTQALIPGDVFAKNSLWKGNOV4m, CG50377-09SEQ ID NO: 551135 bpDNA SequenceORF Start: ATG at 510ORF Stop: TAG at 1044TTAAGAACCAGGTGTCGCTGTCCCTCCGCTCCTGCACATTTCTATTTGGTATTTCAATATGTGACATGAATCTTGAGGCCAGCATGTCACACGTATACAGGGGCTTAAGCTTGTGGGGGCCTTCCCGAGGACAGAGAAAGTGGCCCTAAATGCTATACTTTGATTCCTAAACTCATGAACTTCCCAACAATTCTCATGGAATTCATGAACGACCCAAGCATGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATCTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTAAGTTCAGCAAACTCACCTCCTCCCCTGACTCCCAGATCACTGGCAGGGAAGAGGAGGACAAAAGGGTGTGCAAACCCCTAGGATCAATCTGGCAACTTGGGAGGAGGTTTTCTGTGGGGCCCTGCCTAGGAGCAGCAAGAGGATCCTCATCTGCTAATGAAATAGGCAAANOV4m, CG50377-09SEQ ID NO: 56178 aaMW at 19569.2kDProtein SequenceMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPPICLEVRPSGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSANSPPPLTPRSLAGKRRTKGCANPNOV4n, CG50377-10SEQ ID NO: 573905 bpDNA SequenceORF Start: ATG at 427ORF Stop: TGA at 3079GGATTTGAGAGCCTGAACTTAGCCATACACCAGATCTACCTTTGGACCGCAAAAGGGACCCAGTGCTTCATGAAGCTGGTTTTTTTTGTTTTGTTTTGTTTTTTTTCCGTTGTTTTGTTTCGGCTTTACCAACCTGACTGGGTGTTTTTCAATATCCACCATTCAGACTTTCCTCAACAGCAGAGGATGTGGCAGTGGCAAAGACAAGGGGATGGGGGGAGACGAAAGGGAAAGGGGCCTGCATGAAAGACCATGTCTGTCTTCCTGCTGGTGCCAGTTCCCTGAACCTCATCTTGTTGTTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAJJGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCGTGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCNNAACCACGGCTTCTACCTCCTGGGCAACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGCAGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCAcGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCTTTTTTTGCTAGGTTGAACTGGTACTCCAGCAGCCGCCGAAGCTGGACTGTACTGCTGCCATCTCAGCTCACTGCAACCTCCCTGCCTGATTCCCCTGCCTCAGCCTGCCGAGTGCCTGCGATTGCAGGCGCGCACCGCCACNNTNOV4n, CG50377-10SEQ ID NO: 58884 aaMW at 94718.1kDProtein SequenceMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCXTTASTSWATPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGTRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRPEDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSRVAPSTAPARRMAAGQASRPSAWQRSGPVGDPSTLPGSHRSPKPNOV4o, CG50377-11SEQ ID NO: 593896 bpDNA SequenceORF Start: ATG at 427ORF Stop: TGA at 3073GGATTTGAGAGCCTGAACTTAGCCATACACCAGATCTACCTTTGGACCGCAAAAGGGACCCAGTGCTTCATGAAGCTGGTTTTTTTTGTTTTGTTTTGTTTTTTTTCCGTTGTTTTGTTTCGGCTTTACCAACCTGACTGGGTGTTTTTCAATATCCACCATTCAGACTTTCCTCAACAGCAGAGGATGTGGCAGTGGCAAAGACAAGGGGATGGGGGGAGACGAAAGGGAAAGGGGCCTGCATGAAAGACCATGTCTGTCTTCCTGCTGGTGCCAGTTCCCTGAACCTCATCTTGTTGTTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCGTGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCTTTTTTTGCTAGGTTGAACTGGTACTCCAGCAGCCGCCCAAGCTGGACTGTACTGCTGCCATCTCAGCTCACTGCAACCTCCCTGCCTGATTCCCCTGCCTCAGCCTGCCGAGTGCCTGCGATTGCAGGCGCGCACCGCCACNOV4o, CG50377-11SEQ ID NO: 60882 aa MW at 94686.1kDProtein SequenceMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGTRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSRVAPSTAPARRMAAGQASRPSAWRSGPVGDPSTLPGSHRSPKPNOV4p, SNP13382457 ofSEQ ID NO: 6110136 bpCG50377-01, DNA SequenceORF Start: ATG at 1ORF Stop: TGA at 9313SNP Pos: 3120SNP Change: G to AATGGCGGGCGCCCCTCCCCCCGCCTTGCTGCTGCCTTGCAGTTTGATCTCAGACTGCTGTGCTAGCAATCAGCGACACTCCGTGGGCGTAGGACCCTCCGAGCTAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGTTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTGATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGTCTCAAAATGCCTGCAGTGACAGCCCTCACACCCCAGGCTCTCGCATCCCAGAGAGCATGTCTGGGGACATCTGGAGGCAGAAATGGACTGTACTTGAGATCTGTCGTGACATTAGCAGTTCAGATGCAAGGTCAGGTTCAGTGAGGAAGTCTCCAAAGACTTCTAATGCTGTGGAACTTGTTGCTCCTGGGACAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCACCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTTCTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCACCTTCCGACACAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGATTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGACTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGGTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGCTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGACAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGTGTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTTAACATCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTATACAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCAACGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCAGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGGCGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCGTGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCTTTTTTTGCTAGGTTGAACTGGTACTCCAGCAGCCGCCGAAGCTGGACTGTACTGCTGCCATCTCAGCTCCCACNOV4p, SNP13382457 ofSEQ ID NO: 623104 aaMW at 336746.3kDCG50377-01,SNP Pos: 1040SNP Change: Gly to GlyProtein SequenceMAGAPPPALLLPCSLISDCCASNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGyDLQGSKRITCMKVSDMFAAWSDHRPVCRARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKAPAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVFNIFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGNGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPPAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRPEDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSRVAPSTAPARPMAAGQASRPSAWRSGPVGDPSTLPGSHRSPKPNOV4q, SNP13382458 ofSEQ ID NO: 6310136 bpCG50377-01, DNA SequenceORF Start: ATG at 1ORF Stop: TGA at 9313SNP Pos: 4429SNP Change: A to GATGGCGGGCGCCCCTCCCCCCGCCTTGCTGCTGCCTTGCAGTTTGATCTCAGACTGCTGTGCTAGCAATCAGCGACACTCCGTGGGCGTAGGACCCTCCGAGCTAGTCAAGAAGCAAATTGAGTTGAAGTCTCGAGGTGTGAAGCTGATGCCCAGCAAAGACAACAGCCAGAAGACGTCTGTGTTAACTCAGGTTGGTGTGTCCCAAGGACATAATATGTGTCCAGACCCTGGCATACCCGAAAGGGGCAAAAGACTAGGCTCGGATTTCAGGTTAGGATCCAGCGTCCAGTTCACCTGCAACGAGGGCTATGACCTGCAAGGGTCCAAGCGGATCACCTGTATGAAAGTGAGCGACATGTTTGCGGCCTGGAGCGACCACAGGCCAGTCTGCCGAGCCCGCATGTGTGATGCCCACCTTCGAGGCCCCTCGGGCATCATCACCTCCCCCAATTTCCCCATTCAGTATGACAACAATGCACACTGTGTGTGGATCATCACAGCACTCAACCCCTCCAAGGTGATCAAGCTCGCCTTTGAGGAGTTTGATTTGGAGAGGGGCTATGACACCCTGACGGTCGGTGATGGTGGTCAGGATGGGGACCAGAAGACAGTTCTCTACATGTCTCAAAATGCCTGCAGTGACAGCCCTCACACCCCAGGCTCTCGCATCCCAGAGAGCATGTCTGGGGACATCTGGAGGCAGAAATGGACTGTACTTGAGATCTGTCGTGACATTAGCAGTTCAGATGCAAGGTCAGGTTCAGTGAGGAAGTCTCCAAAGACTTCTAATGCTGTGGAACTTGTTGCTCCTGGGACAGAGATCGAGCAGGGCAGTTGCGGTGACCCTGGCATACCTGCATATGGCCGGAGGGAAGGCTCCCGGTTTCACCACGGTGACACACTCAAGTTTGAGTGCCAGCCCGCCTTTGAGCTGGTGGGACAGAAGGCAATCACATGCCAAAAGAATAACCAATGGTCGGCTAAGAAGCCAGGCTGCGTGTTCTCCTGCTTCTTCAACTTCACCAGCCCGTCTGGGGTTGTCCTGTCTCCCAACTACCCAGAGGACTATGGCAACCACCTCCACTGTGTCTGGCTCATCCTGGCCAGGCCTGAGAGCCGCATCCACCTGGCCTTCAACGACATTGACGTGGAGCCTCAGTTTGATTTCCTGGTCATCAAGGATGGGGCCACCGCCGAGGCGCCCGTCCTGGGCACCTTCTCAGGAAACCAGCTTCCCTCCTCCATCACAAGCAGTGGCCACGTGGCCCGTCTCGAGTTCCAGACTGACCACTCCACAGGGAAGAGGGGCTTCAACATCACTTTTACCACCTTCCGACACAACGAGTGCCCGGATCCTGGCGTTCCAGTAAATGGCAAACGGTTTGGGGACAGCCTCCAGCTGGGCAGCTCCATCTCCTTCCTCTGTGATGAAGGCTTCCTTGGGACTCAGGGCTCAGAGACCATCACCTGCGTCCTGAAGGAGGGCAGCGTGGTCTGGAACAGCGCTGTGCTGCGGTGTGAAGCTCCCTGTGGTGGTCACCTGACTTCGCCCAGCGGCACCATCCTCTCTCCGGGCTGGCCTGGCTTCTACAAGGATGCCTTGAGCTGTGCCTGGGTGATTGAGGCCCAGCCAGGCTACCCCATCAAAATCACCTTCGACAGATTCAAAACCGAGGTCAACTATGACACCCTGGAAGTACGCGATGGGCGGACTTACTCAGCGCCCTTGATCGGGGTTTACCACGGGACCCAGGTTCCCCAGTTCCTCATCAGCACCAGCAACTACCTCTACCTCCTCTTCTCTACCGACAAGAGTCACTCGGACATCGGCTTCCAGCTCCGCTATGAGACTATAACACTGCAGTCAGACCACTGTCTGGATCCAGGAATCCCAGTAAATGGACAGCGTCATGGGAATGACTTCTACGTGGGCGCGCTGGTGACCTTCAGCTGTGACTCGGGCTACACATTAAGTGACGGGGAGCCTCTGGAGTGTGAGCCCAACTTCCAGTGGAGCCGGGCCCTGCCCAGTTGTGAAGCTCTCTGTGGTGGCTTCATTCAAGGCTCCAGTGGGACCATCTTGTCGCCAGGGTTCCCTGACTTCTACCCCAACAACTTGAACTGCACCTGGATTATCGAAACATCTCATGGCAAGGGTGTGTTCTTCACTTTCCACACCTTCCACCTGGAAAGTGGCCATGACTACCTCCTCATCACTGAGAACGGCAGCTTCACCCAGCCCCTGAGGCAGCTAACTGGATCTCGGCTGCCAGCTCCCATCAGCGCTGGGCTCTATGGCAACTTCACTGCCCAGGTCCGCTTCATCTCTGATTTCTCCATGTCATATGAAGGATTCAACATCACCTTCTCAGAGTACGACTTGGAGCCCTGTGAGGAGCCCGAGGTCCCAGCCTACAGCATCCGGAAGGGCTTGCAGTTTGGCGTGGGCGACACCTTGACCTTCTCCTGCTTCCCCGGGTACCGTCTGGAGGGCACCGCCCGCATCACGTGCCTGGGGGGCAGACGGCGCCTGTGGAGCTCGCCTCTGCCAAGGTGTGTTGCTGAGTGTGGGAATTCAGTCACAGGCACTCAGGGTACTTTGCTGTCCCCCAACTTTCCTGTGAACTACAATAACAATCATGAATGCATCTACTCCATCCAGACCCAGCCAGGGAAGGGAATTCAGCTGAAAGCCAGGGCATTCGAACTCTCCGAAGGAGATGTCCTCAAGGTTTATGATGGCAACAACAACTCCGCCCGTTTGCTGGGAGTTTTTAGCCATTCTGAGATGATGGGGGTGACTTTGAACAGCACATCCAGCAGTCTGTGGCTTGATTTCATCACTGATGCTGAAAACACCAGCAAGGGCTTTGAACTGCACTTTTCCAGCTTTGAACTCATCAAATGTGAGGACCCAGGAACCCCCAAGTTTGGCTACAAGGTTCATGATGAAGGTCATTTTGCAGGGAGCTCCGTGTCCTTCAGCTGTGACCCTGGATACAGCCTGCGGGGTAGTGAGGAGCTGCTGTGTCTGAGTGGAGAGCGCCGGACCTGGGACCGGCCTCTGCCCACCTGTGTCGCCGAGTGTGGAGGGACAGTGAGAGGAGAGGTGTCGGGGCAGGTGCTGTCACCCGGGTATCCAGCTCCCTATGAACACAATCTCAACTGCATCTGGACCATCGAAGCAGAGGCCGGCTGCACCATTGGGCTACACTTCCTGGTGTTTGACACAGAGGAGGTTCACGACGTGCTGCGCATCTGGGATGGGCCTGTGGAGAGCGGGGTTCTGCTGAAGGAGCTGAGTGGCCCGGCCCTGCCCAAGGACCTGCATAGCACCTTCAACTCGGTCGTCCTGCAGTTCAGCACTGACTTCTTCACCAGCAAGCAGGGCTTTGCCATTCAATTTTCAGTGTCCACAGCAACGTCCTGCAATGACCCTGGGATCCCGCAGAATGGGAGTCGGAGTGGTGACAGTTGGGAAGCCGGCGACTCCACAGTGTTCCAGTGTGACCCTGGCTACGCGCTGCAGGGAAGTGCAGAGATCAGCTGTGTGAAGATCGAGAACAGGTTCTTCTGGCAGCCCAGCCCGCCAACATGCATCGCTCCCTGCGGGGGAGACCTGACAGGACCATCTGGAGTCATCCTCTCACCAAATTACCCAGAACCCTACCCGCCAGGCAAGGAGTGTGACTGGAAAGTGACCGTCTCACCAGACTACGTCATCGCCCTGGTATTTAACATCTTTAACCTGGAGCCTGGCTATGACTTCCTCCATATCTACGACGGACGGGACTCTCTCAGCCCTCTCATAGGAAGCTTCTATGGCTCCCAGCTCCCAGGCCGCATTGAAAGCAGCAGCAACAGCCTCTTCCTCGCCTTCCGCAGCGATGCATCTGTGAGCAATGCTGGCTTCGTCATTGACTATACAGAAAACCCGCGGGAGTCATGTTTTGATCCTGGTTCCATCAAGAACGGCACACGGGTGGGGTCCGACCTGAAGCTGGGCTCCTCCGTCACCTACTACTGCCACGGGGGCTACGAAGTTGAGGGCACCTCGACCCTGAGCTGCATCCTGGGGCCTGATGGGAAGCCCGTGTGGAACAATCCCCGGCCAGTCTGCACAGCCCCCTGTGGGGGACAGTATGTGGGTTCGGACGGAGTGGTCTTGTCCCCCAACTACCCCCAGAACTACACCAGTGGACAGATCTGCTTGTATTTTGTTACTGTGCCCAAGGACTATGTGGTGTTTGGCCAGTTCGCCTTCTTTCACACGGCCCTCCCAGACGTGGTGGAGGTTCACGACGGCCACAGCCAGCACTCGCGGCTCCTCAGCTCCCTCTCGGGCTCCCATACAGGAGAATCACTGCCCTTGGCCACCTCCAATCAAGTTCTCATTAAGTTCAGCGCCAAAGGCCTCGCACCAGCCGGAGGCTTCCACTTTGTCTACCAAGCGGTTCCTCGAACCAGCGCCACGCAGTGCAGCTCTGTGCCGGAACCCCGCTATGGCAAGAGGCTGGGCAGTGACTTCTCGGTGGGGGCCATCGTCCGCTTCGAATGCAACTCCGGCTATGCCCTGCAGGGGTCGCCAGAGATCGAGTGCCTCCCTGTGCCTGGGGCCTTGGCCCAATGGAATGTCTCAGCGCCCACGTGTGTGGTGCCGTGTGGAGGCAACCTCACAGAGCGCAGGGGCACCATCCTGTCCCCTGGCTTCCCAGAGCCGTACCTCAACAGCCTCAACTGTGTGTGGAAGATCGTGGTCCCCGAAGGCGCTGGCATCCAGATCCAAGTTGTCAGTTTTGTGACAGAGCAGAACTGGGACTCGCTGGAAGTATTTGATGGTGCAGATAACACTGTAACCATGCTGGGGAGTTTCTCAGGAACAACCGTGCCTGCCCTTCTGAACAGCACCTCCAACCAGCTCTACCTTCATTTCTACTCAGATATCAGCGTATCTGCAGCTGGCTTCCACTTGGAGTACAAAACGGTGGGCCTGAGCAGTTGTCCGGAACCTGCTGTGCCCAGTAACGGGGTGAAGACTGGCGAGCGCTACTTGGTGAATGATGTGGTGTCTTTCCAGTGTGAGCCGGGATATGCCCTCCAGGGCCACGCCCACATCTCCTGCATGCCCGGAACAGTGCGGCGATGGAACTACCCTCCTCCACTCTGTATTGCACAGTGTGGGGGAACAGTGGAGGAGATGGAGGGGGTGATCCTGAGCCCCGGCTTCCCAGGCAACTACCCCAGTAACATGGACTGCTCCTGGAAAATAGCACTGCCCGTGGGCTTTGGAGCTCACATCCAGTTCCTGAACTTCTCCACCGAGCCCAACCACGACTACATAGAAATCCGGAATGGCCCCTATGAGACCAGCCGCATGATGGGAAGATTCAGTGGAAGCGAGCTTCCAAGCTCCCTCCTCTCCACGTCCCACGAGACCACCGTGTATTTCCACAGCGACCACTCCCAGAATCGGCCAGGATTCAAGCTGGAGTATCAGGCCTATGAACTTCAAGAGTGCCCAGACCCAGAGCCCTTTGCCAATGGCATTGTGAGGGGAGCTGGCTACAACGTGGGACAATCAGTGACCTTCGAGTGCCTCCCGGGGTATCAATTGACTGGCCACCCTGTCCTCACGTGTCAACATGGCACCAACCGGAACTGGGACCACCCCCTGCCCAAGTGTGAAGTCCCTTGTGGCGGGAACATCACTTCTTCCAACGGCACTGTGTACTCCCCGGGGTTCCCTAGCCCGTACTCCAGCTCCCAGGACTGTGTCTGGCTGATCACCGTGCCCATTGGCCATGGCGTCCGCCTCAACCTCAGCCTGCTGCAGACAGAGCCCTCTGGAGATTTCATCACCATCTGGGATGGGCCACAGCAAACAGCACCACGGCTCGGCGTCTTCACCCGGAGCATGGCCAAGAAAACAGTGCAGAGTTCATCCAACCAGGTCCTGCTCAAGTTCCACCGTGATGCAGCCACAGGGGGGATCTTCGCCATAGCTTTCTCCGCTTATCCACTCACCAAATGCCCTCCTCCCACCATCCTCCCCAACGCCGAAGTCGTCACAGAGAATGAAGAATTCAATATAGGTGACATCGTACGCTACAGATGCCTCCCTGGCTTTACCTTAGTGGGGAATGAAATTCTGACCTGCAAACTTGGAACCTACCTGCAGTTTGAAGGACCACCCCCGATATGTGAAGTGCACTGTCCAACAAATGAGCTTCTGACAGACTCCACAGAGGTGATCCTGAGCCAGAGCTACCCTGGAAGCTATCCCCAGTTCCAGACCTGCTCTTGGCTGGTGAGAGTGGAGCCCGACTATAACATCTCCCTCACAGTGGAGTACTTCCTCAGCGAGAAGCAATATGATGAGTTTGAGATTTTTGATGGTCCATCAGGACAGAGTCCTCTGCTGAAAGCCCTCAGTGGGAATTACTCAGCTCCCCTGATTGTCACCAGCTCAAGCAACTCTGTGTACCTGCGTTGGTCATCTGATCACGCCTACAATCGGAAGGGCTTCAAGATCCGCTATTCAGCCCCTTACTGCAGCCTGCCCAGGGCTCCACTCCATGGCTTCATCCTAGGCCAGACCAGCACCCAGCCCGGGGGCTCCATCCACTTTGGCTGCAACGCCGGCTACCGCCTGGTGGGACACAGCATGGCCATCTGTACCCGGCACCCCCAGGGCTACCACCTGTGGAGCGAAGCCATCCCTCTCTGTCAAGCTCTTTCCTGTGGGCTTCCTGAGGCCCCCAAGAATGGAATGGTGTTTGGCAAGGAGTACACAGTGGGAACCAAGGCCGTGTACAGCTGCAGTGAAGGCTACCACCTCCAGGCAGGCGCTGAGGCCACTGCAGAGTGTCTGGACACAGGCCTATGGAGCAACCGCAATGTCCCACCACAGTGTGTCCCTGTGACTTGTCCTGATGTCAGTAGCATCAGCGTGGAGCATGGCCGATGGAGGCTTATCTTTGAGACACAGTATCAGTTCCAGGCCCAGCTGATGCTCATCTGTGACCCTGGCTACTACTATACTGGCCAAAGGGTCATCCGCTGTCAGGCCAATGGCAAATGGAGCCTCGGGGACTCTACGCCCACCTGCCGAATCATCTCCTGTGGAGAGCTCCCGATTCCCCCCAATGGCCACCGCATCGGAACACTGTCTGTCTACGGGGCAACAGCCATCTTCTCCTGCAATTCCGGATACACACTGGTGGGCTCCAGGGTGCGTGAGTGCATGGCCAATGGGCTCTGGAGTGGCTCTGAAGTCCGCTGCCTTGCTGGACACTGTGGGACTCCTGAGCCCATTGTCAACGGACACATCAATGGGGAGAACTACAGCTACCGGGGCAGTGTGGTGTACCAATGCAATGCTGGCTTCCGCCTGATCGGCATGTCTGTGCGCATCTGCCAGCAGGATCATCACTGGTCGGGCAAGACCCCTTTCTGTGTGCCAATTACCTGTGGACACCCAGGCAACCCTGTCAACGGCCTCACTCAGGGTAACCAGTTTAACCTCAACGATGTGGTCAAGTTTGTTTGCAACCCTGGGTATATGGCTGAGGGGGCTGCTAGGTCCCAATGCCTGGCCAGCGGGCAATGGAGTGACATGCTGCCCACCTGCAGAATCATCAACTGTACAGATCCTGGACACCAAGAAAATAGTGTTCGTCAGGTCCACGCCAGCGGCCCGCACAGGTTCAGCTTCGGCACCACTGTGTCTTACCGGTGCAACCACGGCTTCTACCTCCTGGGCACCCCAGTGCTCAGCTGCCAGGGAGATGGCACATGGGACCGTCCCCGCCCCCAGTGTCTCTTGGTGTCCTGTGGCCATCCGGGCTCCCCGCCTCACTCCCAGATGTCTGGAGACAGTTATACTGTGGGAGCAGTGGTGCGGTACAGCTGCATCGGCAAGCGTACTCTGGTGGGAAACAGCACCCGCATGTGTGGGCTGGATGGACACTGGACTGGCTCCCTCCCTCACTGCTCAGGAACCAGCGTGGGAGTTTGCGGTGACCCTGGGATCCCGGCTCATGGCATCCGTTTGGGGGACAGCTTTGATCCAGGCACTGTGATGCGCTTCAGCTGTGAAGCTGGCCACGTGCTCCGGGGATCGTCAGAGCGCACCTGTCAAGCCAATGGCTCGTGGAGCGGCTCGCAGCCTGAGTGTGGAGTGATCTCTTGTGGGAACCCTGGGACTCCAAGTAATGCCCGAGTTGTGTTCAGTGATGGCCTGGTTTTCTCCAGCTCTATCGTCTATGAGTGCCGGGAAGGATACTACGCCACAGGCCTGCTCAGCCGTCACTGCTCGGTCAATGGTACCTGGACAGGCAGTGACCCTGAGTGCCTCGTCATAAACTGTGGTGACCCTGGGATTCCAGCCAATGGCCTTCGGCTGGGCAATGACTTCAGGTACAACAAAACTGTGACATATCAGTGTGTCCCTGGCTATATGATGGAGTCACATAGAGTATCTGTGCTGAGCTGCACCAAGGACCGGACATGGAATGGAACCAAGCCCGTCTGCAAAGCTCTCATGTGCAAGCCACCTCCGCTCATCCCCAATGGGAAGGTGGTGGGGTCTGACTTCATGTGGGGCTCAAGTGTGACTTATGCCTGCCTGGAGGGGTACCAGCTCTCCCTGCCCGCGGTGTTCACCTGTGAGGGAAATGGGTCCTGGACCGGAGAGCTGCCTCAGTGTTTCCCTGTGTTCTGCGGGGATCCTGGTGTCCCGTCCCGTGGGAGGAGAGAGGACCGAGGCTTCTCCTACAGGTCATCTGTCTCCTTCTCCTGCCATCCCCCTCTGGTGCTGGTGGGCTCTCCACGCAGGTTTTGCCAGTCAGATGGGACATGGAGTGGCACCCAGCCCAGCTGCATAGATCCGACCCTGACCACGTGTGCGGACCCTGGTGTGCCACAGTTTGGGATACAGAACAATTCTCAGGGCTACCAGGTTGGAAGCACAGTCCTCTTCCGTTGTCAAAAAGGCTACCTGCTTCAGGGCTCCACCACCAGGACCTGCCTCCCAAACCTGACCTGGAGTGGAACCCCACCTGACTGTGTCCCCCACCACTGCAGGCAGCCAGAGACGCCAACGCATGCCAACGTCGGGGCCCTGGATTTGCCCTCCATGGGCTACACGCTCATTACTCCTGCCAGGAGGGCTTCTCCCTCAAGGGTGGCTCCGAGCACCGCACCTGCAAGGCGGATGGCAGCTGGACAGGCAAGCCGCCCATCTGCCTGGAGGTCCGGCCCAGTGGGAGACCCATCAACACTGCCCGGGAGCCACCGCTCACCCAAGCCTTGATTCCTGGGGATGTTTTTGCCAAGAATTCCCTGTGGAAAGGGGCCTATGAATACCAGGGGAAGAAGCAGCCAGCCATGCTCAGAGTGACTGGCTTCCAAGTTGCCAACAGCAAGGTCAATGCCACCATGATCGACCACAGTGGCGTGGAGCTGCACTTGGCTGGAACTTACAAGAAAGAAGATTTTCATCTCCTACTCCAGGTGTACCAGATTACAGGGCCTGTGGAGATCTTTATGAATAAGTTCAAAGATGATCACTGGGCTTTAGATGGCCATGTCTCGTCAGAGTCCTCCGGAGCCACCTTCATCTACCAAGGCTCTGTCAAGGGCCAAGGCTTTGGGCAGTTCGGCTTTCAAAGACTGGACCTCAGGCTGCTGGAGTCAGACCCCGAGTCCATTGGCCGCCACTTTGCTTCCAACAGCAGCTCAGTGGCAGCCGCGATCCTGGTGCCTTTCATCGCCCTCATTATTGCGGGCTTCGTGCTCTATCTCTACAAGCACAGGAGAAGACCCAAAGTTCCTTTCAATGGCTATGCTGGCCACGAGAACACCAATGTTCGGGCCACATTTGAGAACCCAATGTACGACCGCAACATCCAGCCCACAGACATCATGGCCAGCGAGGCGGAGTTCACAGTCAGCACAGTGTGCACAGCAGTATAGCCACCCGGCCTGGCCGCTTTTTTTGCTAGGTTGAACTGGTACTCCAGCAGCCGCCGAAGCTGGACTGTACTGCTGCCATCTCAGCTCACTGCAACCTCCCTGCCTGATTCCCCTGCCTCAGCCTGCCGAGTGCCTGCGATTGCAGGCGCGCACCGCCACNOV4q, SNP1 3382458 ofSEQ ID NO: 643104 aaMW at 336647.2kDCG50377-01,SNP Pos: 1477SNP Change: Arg to GlyProtein SequenceMAGAPPPALLLPCSLISDCCASNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNNCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRPISDFSMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVFNIFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPAGGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTPMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSRVAPSTAPARRMAAGQASRPSAWRSGPVGDPSTLPGSHRSPKP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 4B.

TABLE 4BComparison of the NOV4 protein sequences.NOV4a------------------------------------------------------------NOV4bMAGAPPPALLLPCSLISDCCASNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4bVLTQVGVSQGHNMCPDPGIPERGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4bFAAWSDHRPVCRARMCDAHLRGPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFENOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4bEFDLERGYDTLTVGDGGQDGDQKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4bEICRDISSSDARSGSVRKSPKTSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a-------------------------------GQNCTFQLHGPNGTVESPGFPYGYPNYANNOV4bTLKFECQPAFELVGQKAITCQKNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e-------------------------------GQNCTFQLHGPNGTVESPGFPYGYPNYANNOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aCTWTITAEEQHRIQLVFQSFALEEDFDVLSVFDGPPQPENLRTRLTGFQLPATIVSAATTNOV4bLHCVWLILARPESRIHLAFNDIDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eCTWTITAEEQHRIQLVFQSFALEEDFDVLSVFDGPPQPENLRTRLTGFQLPATIVSAATTNOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aLSLPLISDYAVSAQGFHATYEVLPSHTCGNPGRLPNGIQQGSTFNLGDKVRYSCNLGFFLNOV4bNVARLEFQTDHSTGKRGFNITFTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eLSLRLISDYAVSAQGFHATYEVLPSHTCGNPGRLPNGIQQGSTFNLGDKVRYSCNLGFFLNOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aEGHAVLTCHAGSENSATWDFPLPSCRADDACGGTLRGQSGIISSPHFPSEYHNNADCTWTNOV4bLGTQGSETITCVLKEGSVVWNSAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVNOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eEGHAVLTCHAGSENSATWDFPLPSCRADDACGGTLRGQSGIISSPHFPSEYHNNADCTWTNOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aILAELGDTIALVFIDFQLEDGYDFLEVTGTEGSSLWFTGASLPAPVISSKNWLRLHFTSDNOV4bIEAQPGYPIKITFDRFKTEVNYDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLNOV4c--------------------------------------MAGAPPPALLLPCSLISDCCASNOV4d--------------------------------------MAGAPPPALLLPCSLISDCCASNOV4eILAELGDTIALVFIDFQLEDGYDFLEVTGTEGSSLWFTGASLPAPVISSKNWLRLHFTSDNOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aGNHRQRGFSAQYQVKKQIELKSRGVKLMPSKDNSQKTSVVTQVGVSQGHNMCPDPGIPERNOV4bFSTDKSHSDIGFQLRYETITLQSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDNOV4cNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNMCPDPGIPERNOV4dNQRHSVGVGPSELVKKQIELKSRGVKLMPSKDNSQKTSVLTQVGVSQGHNMCPDPGIPERNOV4eGNHRQRGFSAQYQVKKQIELKSRGVKLMPSKDNSQKTSVVTQVGVSQGHNMCPDPGIPERNOV4f---------------------------MPSKDNSQKTSVVTQVGVSQGHNMCPDPGIPERNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRAPMCDAHLRCNOV4bGEPLECEPNFQWSRALPSCEALCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGNOV4cGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRARMCDAHLRGNOV4dGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRARMCDAHLRGNOV4eGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRAPMCDAHLRGN0V4fGKRLGSDFRLGSSVQFTCNEGYDLQGSKRITCMKVSDMFAAWSDHRPVCRAENCDAHLRGNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQNOV4bVFFTFHTFHLESGHDYLLITENGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSNOV4cPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQNOV4dPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQNOV4ePSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQNOV4fPSGIITSPNFPIQYDNNAHCVWIITALNPSKVIKLAFEEFDLERGYDTLTVGDGGQDGDQNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aKTVLYMLTGTSVPDLIVSTNHQMWLLFQTDG---------------------SG------NOV4bMSYEGFNITFSEYDLEPCEEPEVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGNOV4cKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTNOV4dKTVLYMSQNACSDSPHTPGSRIPESMSGDIWRQKWTVLEICRDISSSDARSGSVRKSPKTNOV4eKTVLYMLTGTSVPDLIVSTNHQMWLLFQTDG----------------------------SNOV4fKTVLYMLTGTSVPDLIVSTNHQMWLLFQTDG----------------------S------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a-SSLGFKASYEEIEQGSCGDPGIPAYGRREGSRFRHGDTLKFECQPAFELVGQKAITCQKNOV4bGRRPLWSSPLPRCVAECGNSVTGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARNOV4cSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNOV4dSNAVELVAPGTEIEQGSCGDPGIPAYGRREGSRFHHGDTLKFECQPAFELVGQKAITCQKNOV4eGSSLGFKASYEEIEQGSCGDPGIPAYGRREGSRFRHGDTLKFECQPAFELVGQKAITCQKNOV4fGSSLGFKASYEEIEQGSCGDPGIPAYGRREGSRFRHGDTLKFECQPAFELVGQKAITCQKNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aNNQWSAKKPGCVCSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDINOV4bAFELSEGDVLKVYDGNNNSARLLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELNOV4cNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDINOV4dNNQWSAKKPGCVFSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDINOV4eNNQWSAKKPGCVCSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDINOV4fNNQWSAKKPGCVCSCFFNFTSPSGVVLSPNYPEDYGNHLHCVWLILARPESRIHLAFNDINOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFNOV4bHFSSFELIKCEDPGTPKFGYKVHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDNOV4cDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFNOV4dDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFNOV4eDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHvARLEFQTDHSTGKRGFNITFNOV4fDVEPQFDFLVIKDGATAEAPVLGTFSGNQLPSSITSSGHVARLEFQTDHSTGKRGFNITFNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aTSESSNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSNOV4bRPLPTCVAECGGTVRGEVSGQVLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEENOV4cTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSNOV4dTTFRHNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSNOV4eTSESSNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSNOV4fTSESSNECPDPGVPVNGKRFGDSLQLGSSISFLCDEGFLGTQGSETITCVLKEGSVVWNSNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYNOV4bVHDVLRIWDGPVESGVLLKELSGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTNOV4cAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYNOV4dAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYNOV4eAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYNOV4fAVLRCEAPCGGHLTSPSGTILSPGWPGFYKDALSCAWVIEAQPGYPIKITFDRFKTEVNYNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYEAITLQNOV4bATSCNDPGIPQNGSRSGDSWEAGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCNOV4cDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQNOV4dDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYETITLQNOV4eDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYEAITLQNOV4fDTLEVRDGRTYSAPLIGVYHGTQVPQFLISTSNYLYLLFSTDKSHSDIGFQLRYEAITLQNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALNOV4bIAPCGGDLTGPSGVILSPNYPEPYPPGKECDWKVTVSPDYVIALVFNIFNLEPGYDFLHINOV4cSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALNOV4dSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALNOV4eSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALNOV4fSDHCLDPGIPVNGQRHGNDFYVGALVTFSCDSGYTLSDGEPLECEPNFQWSRALPSCEALNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENNOV4bYDGRDSLSPLIGSFYGSQLPGRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPNOV4cCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENNOV4dCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENNOV4eCGGFIQGSSGTILSPGFPDFYPNNLNCTWITETSHGKGVFFTFHTFHLESGHDYLLITENNOV4fCGGFIQGSSGTILSPGFPDFYPNNLNCTWIIETSHGKGVFFTFHTFHLESGHDYLLITENNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPENOV4bGSIKNGTRVGSDLKLGSSVTYYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGNOV4cGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPENOV4dGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPENOV4eGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPENOV4fGSFTQPLRQLTGSRLPAPISAGLYGNFTAQVRFISDFSMSYEGFNITFSEYDLEPCEEPENOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGPRRLWSSPLPRCVAECGNSVTNOV4bQYVGSDGVVLSPNYPQNYTSGQICLYFVTVPKDYVVFGQFAFFHTALNDVVEVHDGHSQHNOV4cVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTNOV4dVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTNOV4eVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTNOV4fVPAYSIRKGLQFGVGDTLTFSCFPGYRLEGTARITCLGGRRRLWSSPLPRCVAECGNSVTNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLNOV4bSRLLSSLSGSHTGESLPLATSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPNOV4cGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLNOV4dGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLNOV4eGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLNOV4fGTQGTLLSPNFPVNYNNNHECIYSIQTQPGKGIQLKARAFELSEGDVLKVYDGNNNSARLNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVNOV4bRYGKRLGSDFSVGATVRFECNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTNOV4cLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVNOV4dLGVFSHSEMNGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVNOV4eLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVNOV4fLGVFSHSEMMGVTLNSTSSSLWLDFITDAENTSKGFELHFSSFELIKCEDPGTPKFGYKVNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVNOV4bERRGTILSPGFPEPYLNSLNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTNOV4cHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVNOV4dHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVNOV4eHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVNOV4fHDEGHFAGSSVSFSCDPGYSLRGSEELLCLSGERRTWDRPLPTCVAECGGTVRGEVSGQVNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSNOV4bMLGSFSGTTVPALLNSTSNQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTNOV4cLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSNOV4dLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSNOV4eLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSNOV4fLSPGYPAPYEHNLNCIWTIEAEAGCTIGLHFLVFDTEEVHDVLRIWDGPVESGVLLKELSNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSGSTATSCNDPGIPQNGSRSGDSWEANOV4bGERYLVNDVVSFQCEPGYALQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSNOV4cGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNGSRSGDSWEAN0V4dGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSVSTATSCNDPGIPQNGSRSGDSWEANOV4eGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSGSTATSCNDPGIPQNGSRSGDSWEANOV4fGPALPKDLHSTFNSVVLQFSTDFFTSKQGFAIQFSGSTATSCNDPGIPQNGSRSGDSWEANOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPENOV4bPGFPGNYPSNMDCSWKIALPVGFGAHIQFLNFSTEPNHDYIEIRNGPYETSRMMGRFSGSNOV4cGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPENOV4dGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPENOV4eGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPENOV4fGDSTVFQCDPGYALQGSAEISCVKIENRFFWQPSPPTCIAPCGGDLTGPSGVILSPNYPENOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aPYPPGKECDWKVTVSPDYVIALVLFPSFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGNOV4bELPSSLLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQNOV4cPYPPGKECDWKVTVSPDYVIALVFN-IFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGNOV4dPYPPGKECDWKVTVSPDYVIALVFN-IFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGNOV4ePYPPGKECDWKVTVSPDYVIALVLFPSFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGNOV4fPYPPGKECDWKVTVSPDYVIALVLFPSFNLEPGYDFLHIYDGRDSLSPLIGSFYGSQLPGNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aRIESSSNSLFLAFRSDASVSNAGFVIDFPENPRESCFDPGSIKNGTRVGSDLKLGSSVTYNOV4bSVTFECLPGYQLTGHPVLTCQHGTNRNWDHPLPKCEVPCGGNITSSNGTVYSPGFPSPYSNOV4cRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYNOV4dRIESSSNSLFLAFRSDASVSNAGFVIDYTENPRESCFDPGSIKNGTRVGSDLKLGSSVTYNOV4eRIESSSNSLFLAFRSDASVSNAGFVIDFPENPRESCFDPGSIKNGTRVGSDLKLGSSVTYNOV4fRIESSSNSLFLAFRSDASVSNAGFVIDFPENPRESCFDPGSIKNGTRVGSDLKLGSSVTYNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j--------------------------------KLSCFDPGSIKNGTRVGSDLKLGSSVTYNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGNOV4bSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKTVQSNOV4cYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGNOV4dYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGNOV4eYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGNOV4fYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jYCHGGYEVEGTSTLSCILGPDGKPVWNNPRPVCTAPCGGQYVGSDGVVLSPNYPQNYTSGNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aQICLYFVTVPKDYGVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTG-ESLPLANOV4bSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGD-IVRYRCNOV4cQICLYFVTVPKDY-VVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTG-ESLPLANOV4dQICLYFVTVPKDY-VVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTG-ESLPLANOV4eQICLYFVTVPKDYGVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTG-ESLPLANOV4fQICLYFVTVPKDYGVVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTGGESLPLANOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jQICLYFVTVPKDY-VVFGQFAFFHTALNDVVEVHDGHSQHSRLLSSLSGSHTG-ESLPLANOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aTSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFENOV4bLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQFQTNOV4cTSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFENOV4dTSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFENOV4eTSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKPLGSDFSVGAIVRFENOV4fTSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFENOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jTSNQVLIKFSAKGLAPARGFHFVYQAVPRTSATQCSSVPEPRYGKRLGSDFSVGAIVRFENOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aCNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNOV4bCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSSSNNOV4cCNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNOV4dCNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNOV4eCNSGYALQGSPEIECLPVPGAIAQWNVSAPTCVVPCGGNLTERRGTTLSPGFPEPYLNSLNOV4fCNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jCNSGYALQGSPEIECLPVPGALAQWNVSAPTCVVPCGGNLTERRGTILSPGFPEPYLNSLNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNNOV4bSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYRLVNOV4CNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNNOV4dNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNNOV4eNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNN0V4fNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jNCVWKIVVPEGAGIQIQVVSFVTEQNWDSLEVFDGADNTVTMLGSFSGTTVPALLNSTSNNOV4k------------------------------------------------------------Nov41------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYANOV4bGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHNOV4cQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYANOV4dQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYANOV4eQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYANOV4fQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYANOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jQLYLHFYSDISVSAAGFHLEYKTVGLSSCPEPAVPSNGVKTGERYLVNDVVSFQCEPGYANOV4k------------------------------------------------------------N0V4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n---MAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHNOV4o---MAICTRHPQGYHLWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGYHNOV4aLQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEENEGVILSPGFPGNYPSNMDCSWKIALNOV4bLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLNOV4cLQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALNOV4dLQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALNOV4eLQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNNDCSWKIALNOV4fLQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEEMEGVILSPGFPGNYPSNMDCSWKIALNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jLQGHAHISCMPGTVRRWNYPPPLCIAQCGGTVEENEGVILSPGFPGNYPSNMDCSWKIALNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4nLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLNOV4oLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLNLNOV4aPVGFGAHIQFLNFSTEPNHDYIEIRNGP-------------YETSRMMGRFSGSELPSSLNOV4bICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCDELPIPPNGHRIGTLSVYGATAIFNOV4cPVGFGAHIQFLNFSTEPNHDYIEIRNGP-------------YETSRMNGRFSGSELPSSLNOV4dPVGFGAHIQFLNFSTEPNHDYIEIRNGP-------------YETSRMMGRFSGSELPSSLNOV4ePVGFGAHIQFLNFSTEPNHDYIEIRNGP-------------YETSRMMGRFSGSELPSSLNOV4fPVGFGAHIQFLNFSTEPNHDYIEIRNGP-------------YETSPMMGRFSGSELPSSLNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4jPVGFGAHIQFLNFSTEPNHDYIEIRNGP-------------YETSRMMGRFSGSELPSSLNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------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------------------------------KLECPDPEPFANGIVRGAGYNVGQSVTFECNOV4h------------------------------KLECPDPEPFANGIVRGAGYNVGQSVTFECNOV4i------------------------------KLECPDPEPFANGIVRGAGYNVGQSVTFECNOV4jLSTSHETTVYFHSDHSQNRPGFKLEYQAYELQECPDPEPFANGIVRGAGYNVGQSVTFECNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4nSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNOV4oSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNOV4aLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4bNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPNOV4cLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4dLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4eLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4fLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4gLPGYQLTGHPVLTCQHGTNRN----------WDHSLPKCEVPCGGNITSSNGTVYSPGFPNOV4hLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4iLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4jLPGYQLTGHPVLTCQHGTNRN----------WDHPLPKCEVPCGGNITSSNGTVYSPGFPNOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4nNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPNOV4oNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPNOV4aSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4bGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRNOV4cSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4dSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4eSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4fSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4gSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4hSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4iSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4jSPYSSSQDCVWLITVPIGHGVRLNLSLLQTEPSGDFITIWDGPQQTAPRLGVFTRSMAKKNOV4k------------------------------------------------------------NOV4l---AEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRNOV4m----------------------------------------------------MGYTLIYSNOV4nGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRNOV4oGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRNOV4aTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4bC--NHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDS--YTVGAVVRNOV4cTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4dTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4eTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4fTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4gTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4hTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4iTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTTLPNAEVVTENEEFNIGDIVRNOV4jTVQSSSNQVLLKFHRDAATGGIFAIAFSAYPLTKCPPPTILPNAEVVTENEEFNIGDIVRNOV4k------------------------------------------------------------NOV4lC--NHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDS--YTVGAVVRNOV4mC-QEGFSLKGGSEHRTCKADGSWTGKPPICLEVR---PSGRPINTAREPP--------LTNOV4nCX-TTASTSWATPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDS--YTVGAVVRNOV4oC--NHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDS--YTVGAVVRNOV4aYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4bYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMNOV4cYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4dYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4eYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4fYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4gYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4hYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4iYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4jYRCLPGFTLVGNEILTCKLGTYLQFEGPPPICEVHCPTNELLTDSTGVILSQSYPGSYPQNOV4k------------------------------------------------------------NOV4lYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMNOV4mQALIPGDVFAKNSLWKGAYEYQGKKQ-PAMLRVTGFQVANSKVNATMIDHSG-VELHLAGNOV4nYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMNOV4oYSCIGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVNNOV4aFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4bRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPG--------TPSNARVVFSDGNOV4cFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4dFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4eFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4fFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4gFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4hFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4iFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4jFQTCSWLVRVEPDYNISLTVEYFLSEKQYDEFEIFDGPSGQSPLLKALSGNYSAPLIVTSNOV4k------------------------------------------------------------NOV4lRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPG--------TPSNARVVFSDGNOV4mTYKKEDFHLLLQVYQITGPVEIFMN-KFKDDHWALDGHVS--------SANSPPPLTPRSNOV4nRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPG--------TPSNARVVFSDGNOV4oRFSCEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPG--------TPSNARVVFSDGNOV4aSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYNOV4bLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGS----------D---------------NOV4cSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYNOV4dSSNSVYLRWSSDHAYNRKGFKIRYSALSCGLP----------------------------NOV4eSSNSVYLRWSSDHAYNRKGFKIRYSALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGYHNOV4fSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYNOV4gSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYNOV4hSSNSVYLRWSSDHAYNRKGFKIRYSAPYCSLPRAPLHGFILGQTSTQPGGSIHFGCNAGYNOV4iSSNSVYLRWSSDHAYNRKGFKIRYSA----------------------------------NOV4jSSNSVYLRWSSDHAYNRKGFKIRYVD----------------------------------NOV4k------------------------------------------------------------NOV4lLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGS----------D---------------NOV4mLAGKRRTKGCANP-----------------------------------------------NOV4nLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGS----------D---------------NOV4oLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGS----------D---------------NOV4aRLVGHSMAICTRHPQGYH------------------------------------------NOV4b------------------------------------------------------------NOV4cRLVGHSMAICTRHPQGYH------------------------------------------NOV4d------------------------------------------------------------NOV4eLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMLNOV4fRLVGHSMAICTRHPQGYHLWSEAIPLCQALSCGLPEAP----------------------NOV4gRLVGHSMAICTRHPQGYH------------------------------------------NOV4hRLVGHSMAICTRHPQGYH------------------------------------------NOV4i---------LS-------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAIFNOV4f------------------------------------KNGMVFGKEYTVGTKAMYSCSEGYNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVTYQCNOV4fHLQAGAEATAECLDTGLWSNRNVPPQCVPVTCPDVSSISVEHGRWRLIFETQYQFQAQLMNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eNAGFRLIGMSVRICQQDHHWSGKTPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPN0V4fLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGTLSVYGATAINOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eGYMAEGAARSQCLASGQWSDMLPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRNOV4fFSCNSGYTLVGSRVRECMANGLWSGSEVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eCNHGFYLLGTPVLSCQGDGTWDRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCINOV4fCNAGFRLIGMSVRICQQDHHWSGKTPFCVLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCINOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCNOV4fGKRTLVGNSTRMCGLDGHWTGSLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a------------------------------------------------------------NOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECRNOV4fEAGHVLRGSSERTCQANGSWSGSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECRNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4a-----------------LWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGNOV4b-----------------------PECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGNOV4c-----------------LWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGNOV4d----------------------------------EAPKNGMVFGKEYTVGTKAMYSCSEGNOV4eEGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGNOV4fEGYYATGLLSRHCSVNGTWTGSDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGNOV4g-----------------LWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAMYSCSEGNOV4h-----------------LWSEAIPLCQALSCGLPEAPKNGMVFGKEYTVGTKAVYSCSEGNOV4i------------------------------CGLPEAPKNGMVFGKEYTVGTKAMYSCSEGNOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l-----------------------PECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGNOV4m------------------------------------------------------------NOV4n-----------------------PECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGNOV4o-----------------------PECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGNOV4aYHLQAGAEATAECLDTGLWSNRNVPPQCVP---------VTCPDVSSISVEHGRWRLIFENOV4bYMMESHRVSVLSCTKDRThNGTKPVCKALMCK--------PPPLI--------PNGKVVGNOV4cYHLQAGAEATAECLDTGLWSNRNVPPQCVRESSGNGGGSVTCPDVSSISVEHGRWRLIFENOV4dYHLQAGAEATAECLDTGLWSNRNVPPQCVP---------VTCPDVSSISVEHGRWRLIFENOV4eYMMESHRVSVLSCTKDRTWNGTKPVCKALMCK--------PPPLIP--------NGKVVGNOV4fYMMESHRVSVLSCTKDRTWNGTKPVCKALMCK--------PPPLIP--------NGKVVGNOV4gYHLQAGAEATAECLDTGLWSNRNVPPQCVP---------VTCPDVSSISVEHGRWRLIFENOV4hYHLQAGAEATAECLDTGLWSNRNVPPQCVP---------VTCPDVSSISVEHGRWRLIFENOV4iYHLQAGAEATAECLDTGLWSNPNVPPQCVP---------VTCPDVSSISVEHGRWRPIFENOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4lYMMESHRVSVLSCTKDRTWNGTKPVCKALMCK--------PPPLI--------PNGKVVGNOV4m------------------------------------------------------------NOV4nYMMESHRVSVLSCTKDRTWNGTKPVCKALMCK--------PPPLI--------PNGKVVGNOV4oYMMESHRVSVLSCTKDRTWNGTKPVCKALMCK--------PPPLI--------PNGKVVGNOV4aTQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGNOV4bSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTG--ELPQCFPVFCGDPGVPSRGRREDNOV4cTQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGNOV4dTQYQFQAQLMLICDPGYYYTGQRVIRCQMJGKWSLGDSTPTCRIISCGELPIPPNGHRIGNOV4eSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTG--ELPQCFPVFCGDPGVPSRGRREDNOV4fSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTG--ELPQCFPVFCGDPGVPSRGRREDNOV4gTQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGNOV4hTQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGNOV4iTQYQFQAQLMLICDPGYYYTGQRVIRCQANGKWSLGDSTPTCRIISCGELPIPPNGHRIGNOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4lSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTG--ELPQCFPVFCGDPGVPSRGRREDNOV4m------------------------------------------------------------NOV4nSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTG--ELPQCFPVFCGDPGVPSRGRREDNOV4oSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTG--ELPQCFPVFCGDPGVPSRGRREDNOV4aTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGS------------------------NOV4bRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQP---------------------SNOV4cTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGSEVRCLATQTKLHSIFYKLLFDVLSNOV4dTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGS------------------------NOV4eRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQP---------------------SNOV4fRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQP---------------------SNOV4gTLSVYGATATFSCNSGYTLVGSRVRECMANGLWSGS------------------------NOV4hTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGS------------------------NOV4iTLSVYGATAIFSCNSGYTLVGSRVRECMANGLWSGS------------------------NOV4j------------------------------------------------------------NOV4k-----KSSVSFSCHPPLVLVGSPRLRFCQSDGTWSGTQP--------------------SNOV4lRGFSYRSSVSFSCHPPLVLVGSPRLRFCQSDGTWSGTQP---------------------NOV4m------------------------------------------------------------NOV4nRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQP---------------------SNOV4oRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQP---------------------SNOV4a-EVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTNOV4bCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4cSPSLTKAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTNOV4d-EVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRTCQQDHHWSGKTNOV4eCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4fCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4g-EVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRTCQQDHHWSGKTNOV4h-EVRCIAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTNOV4i-EVRCLAGHCGTPEPIVNGHINGENYSYRGSVVYQCNAGFRLIGMSVRICQQDHHWSGKTNOV4j------------------------------------------------------------NOV4kCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4lCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4m------------------------------------------------------------NOV4nCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4oCIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPNOV4aPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEG--AARSQCLASGQWSDMLNOV4bPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSR--VAPSTAPARRMAAGQANOV4cPFCVHVKQQLLLLLLLLCDDD----DDE--------------------------------NOV4dPFCQLPVDTQATLSTASLRVTS-LTSTMWSSLFATLGIWLRG--LLGPNAWPAGNGVTCCNOV4ePDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPNOV4fPDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPNOV4gPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEG--AARSQCLASGQWSDMLNOV4hPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEG--AARSQCLASGQWSDMLNOV4iPFCVPITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEG--AARSQCLASGQWSDMLNOV4j------------------------------------------------------------NOV4kPDCVP-------------------------------------------------------NOV4lPDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPNOV4m------------------------------------------------------------NOV4nPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSR--VAPSTAPARRMAAGQANOV4oPDCVPHHCRQPETPTHANVGALDLPSMGYTLITPARRASPSR--VAPSTAPARRMAAGQANOV4aPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDNOV4bSRPSAWR-SGPVGDPSTLPGSHRSPKP---------------------------------NOV4c-DDGSGAITCGHPGNPVNGLTQGNQFNLNDVVKFVCNPGYMAEGAARSQCLASGQWSDMLNOV4dPPAESSTVQILDTKKIVFVRSTPAARTGSASAPLCLTGATTASTSWAPQCSAARENAHGTNOV4ePICLEVRPNGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANOV4fPICLEVRPNGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANOV4gPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDNOV4hPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDNOV4iPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDNOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4lPICLEVRPSGRPINIAREPPLTQALIPGDVFAKNSLWKG---------------------NOV4m------------------------------------------------------------NOV4nSRPSAWQRSGPVGDPSTLPGSHRSPKP---------------------------------NOV4oSRPSAWR-SGPVGDPSTLPGSHRSPKP---------------------------------NOV4aRPRPQCLLVSCGHPGSPPHSQMSGDSYTVGAVVRYSCIGKRTLVGNSTRMCGLDGHWTGSNOV4b------------------------------------------------------------NOV4cPTCRIINCTDPGHQENSVRQVHASGPHRFSFGTTVSYRCNHGFYLLGTPVLSCQGDGTWDNOV4dVPAPSVSVSSGVLWPSGLPASLPDVWRQLYCGSSGAVQLHRQAYSGGKQHPHVWAGWTLDNOV4eNSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSNOV4fNSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSNOV4gRPRPQCLCKVD-------------------------------------------------NOV4hRPRPQCLCKVD-------------------------------------------------NOV4iRPRPQCLCKVD-------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aLPHCSGTSVGVCGDPGIPAHGIRLGDSFDPGTVMRFSCEAGHVLRGSSERTCQANGSWSGNOV4b------------------------------------------------------------NOV4cRPRPQCLCK---------------------------------------------------NOV4dWLPPSLLRNQRGSLR---------------------------------------------NOV4eESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNSSSVAAAILVPFIALNOV4fESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNSSSVAAAILVPFIALNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aSQPECGVISCGNPGTPSNARVVFSDGLVFSSSIVYECREGYYATGLLSRHCSVNGTWTGSNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eIIAGFVLYLYKHRRRPKVPFNGYAGHENTNVPATFENPMYDRNIQPTDIMASEAEFTVSTNOV4fIIAGFVLYLYKHRRRPKVPFNGYAGHENTNVRATFENPMYDRNIQPTDIMASEAEFTVSTNOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aDPECLVINCGDPGIPANGLRLGNDFRYNKTVTYQCVPGYMMESHRVSVLSCTKDRTWNGTNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4eVCTAV-------------------------------------------------------NOV4fVCTAV-------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aKPVCKALMCKPPPLIPNGKVVGSDFMWGSSVTYACLEGYQLSLPAVFTCEGNGSWTGELPNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aQCFPVFCGDPGVPSRGRREDRGFSYRSSVSFSCHPPLVLVGSPRRFCQSDGTWSGTQPSCNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aIDPTLTTCADPGVPQFGIQNNSQGYQVGSTVLFRCQKGYLLQGSTTRTCLPNLTWSGTPPNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aDCVPHHCRQPETPTHANVGALDLPSMGYTLIYSCQEGFSLKGGSEHRTCKADGSWTGKPPNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aICLEVRPNGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------Nov4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSENOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aSSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRBFASNSSSVAAAILVPFIALINOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4f------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aIAGFVLYLYKHRRRPKVPFNGYAGHENTNVRATFENPMYDRNIQPTDIMASEAEFTVSTVNOV4b------------------------------------------------------------NOV4c------------------------------------------------------------NOV4d------------------------------------------------------------NOV4e------------------------------------------------------------NOV4g------------------------------------------------------------NOV4h------------------------------------------------------------NOV4i------------------------------------------------------------NOV4j------------------------------------------------------------NOV4k------------------------------------------------------------NOV4l------------------------------------------------------------NOV4m------------------------------------------------------------NOV4n------------------------------------------------------------NOV4o------------------------------------------------------------NOV4aCTAVNOV4b----NOV4d----NOV4e----NOV4f----NOV4g----NOV4h----NOV4i----NOV4j----NOV4k----NOV4l----NOV4m----NOV4n----NOV4o----NOV4a(SEQ ID NO: 32)NOV4b(SEQ ID NO: 34)NOV4c(SEQ ID NO: 36)NOV4d(SEQ ID NO: 38)NOV4e(SEQ ID NO: 40)NOV4f(SEQ ID NO: 42)NOV4g(SEQ ID NO: 44)NOV4h(SEQ ID NO: 46)NOV4i(SEQ ID NO: 48)NOV4j(SEQ ID NO: 50)NOV4k(SEQ ID NO: 52)NOV4l(SEQ ID NO: 54)NOV4m(SEQ ID NO: 56)NOV4n(SEQ ID NO: 58)NOV4o(SEQ ID NO: 60)


Further analysis of the NOV4a protein yielded the following properties shown in Table 4C.

TABLE 4CProtein Sequence Properties NOV4aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 15; peak value 1.77PSG score:−2.63GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −8.90possible cleavage site: between 50 and 51>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 3Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 3472-3488−11.52PERIPHERALLikelihood = 0.79 (at 1806)ALOM score: −11.52 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 3479Charge difference: 7.0 C(5.5)-N(−1.5)C > N: C-terminal side will be inside>>> Single TMS is located near the C-terminus>>> membrane topology: type Nt (cytoplasmic tail 1 to 3471)MITDISC:discrimination of mitochondrial targeting seqR content: 0Hyd Moment(75):6.28Hyd Moment(95): 5.99G content:3D/E content: 1S/T content:2Score: −5.81Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: KHRR (3) at 3492pat4: HRRR (3) at 3493pat4: RRRP (4) at 3494pat4: RRPK (4) at 3495pat7: PERGKRL (4) at 327pat7: PSRGRRE (4) at 3135bipartite: nonecontent of basic residues: 6.9%NLS Score: 1.09KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: foundILPN at 2237RNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: GQNCTFPrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 474LL at 818LL at 956LL at 1087LL at 1141LL at 1228LL at 1316LL at 1664LL at 1854LL at 2027LL at 2164LL at 2207LL at 2298LL at 2361LL at 2807LL at 2829LL at 2988LL at 3222LL at 3390LL at 3391checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: nuclear26.1%: cytoplasmic 8.7%: Golgi 8.7%: mitochondrial 8.7%: endoplasmic reticulum 4.3%: vacuolar 4.3%: vesicles of secretory system 4.3%: peroxisomal>> prediction for CG50377-04 is nuc (k = 23)


A search of the NOV4a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 4D.

TABLE 4DGeneseq Results for NOV4aNOV4aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG79168Human cub and sushi domain283 . . . 32732943/3019 (97%)0.0containing protein #1 - Homo sapiens, 36 . . . 30522958/3019 (97%)3104 aa. [WO200264791-A2, 22-AUG-2002]ABG79169Human cub and sushi domain283 . . . 28292498/2634 (94%)0.0containing protein #2 - Homo sapiens, 36 . . . 26672512/2634 (94%)2669 aa. [WO200264791-A2, 22-AUG-2002]AAE20789Human C3b/C4b complement receptor437 . . . 35462010/3110 (64%)0.0like protein #2 - Homo sapiens, 3100 1 . . . 31002479/3110 (79%)aa. [WO200210199-A2, 07-FEB-2002]AAE20901Human C3b/C4b complement receptor437 . . . 35462009/3110 (64%)0.0like protein #2, alternative version - 1 . . . 31002478/3110 (79%)Homo sapiens, 3100 aa.[WO200210199-A2, 07-FEB-2002]AAE20788Rat C3b/C4b complement receptor like442 . . . 35461998/3105 (64%)0.0protein - Rattus rattus, 3095 aa. 1 . . . 30952474/3105 (79%)[WO200210199-A2, 07-FEB-2002]


In a BLAST search of public sequence databases, the NOV4a protein was found to have homology to the proteins shown in the BLASTP data in Table 4E.

TABLE 4EPublic BLASTP Results for NOV4aNOV4aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ923L3CSMD1 - Mus musculus (Mouse),1 . . . 35462301/3546 (64%)0.03564 aa.29 . . . 3564 2835/3546 (79%)AAK73475CUB and sushi multiple domains 11 . . . 35462298/3548 (64%)0.0protein - Homo sapiens (Human),29 . . . 3566 2830/3548 (78%)3566 aa.CAD26764Sequence 6 from Patent437 . . . 3546 2010/3110 (64%)0.0WO0210199 - Homo sapiens1 . . . 31002479/3110 (79%)(Human), 3100 aa (fragment).CAD26836Sequence 3 from Patent442 . . . 3546 1998/3105 (64%)0.0WO0210199 - Rattus rattus (Black1 . . . 30952474/3105 (79%)rat), 3095 aa (fragment).CAD26763Sequence 1 from Patent469 . . . 3546 1987/3078 (64%)0.0WO0210199 - Homo sapiens2 . . . 30692451/3078 (79%)(Human), 3069 aa.


PFam analysis predicts that the NOV4a protein contains the domains shown in the Table 4F.

TABLE 4FDomain Analysis of NOV4aIdentities/SimilaritiesPfamNOV4a Matchfor theExpectDomainRegionMatched RegionValueCUB 4 . . . 10939/116 (34%)   2e−2780/116 (69%) sushi117 . . . 17422/65 (34%)4.7e−1046/65 (71%)CUB180 . . . 28145/116 (39%)   5e−2879/116 (68%) sushi321 . . . 37824/65 (37%)3.7e−1144/65 (68%)CUB383 . . . 49143/118 (36%) 1.7e−3084/118 (71%) sushi499 . . . 55219/63 (30%)4.9e−1240/63 (63%)CUB554 . . . 66143/116 (37%)   2e−3381/116 (70%) sushi669 . . . 72616/65 (25%)5.7e−0839/65 (60%)CUB730 . . . 83546/116 (40%) 1.2e−3280/116 (69%) sushi845 . . . 89817/63 (27%)1.8e−0836/63 (57%)CUB 902 . . . 100940/119 (34%) 1.7e−1979/119 (66%) sushi1017 . . . 107221/62 (34%)1.8e−1044/62 (71%)CUB1076 . . . 118134/117 (29%) 1.6e−2481/117 (69%) sushi1189 . . . 124517/65 (26%)5.1e−1045/65 (69%)CUB1249 . . . 135542/116 (36%) 1.1e−2173/116 (63%) sushi1363 . . . 141920/64 (31%)2.1e−0640/64 (62%)CUB1423 . . . 152954/117 (46%) 4.2e−3989/117 (76%) sushi1537 . . . 159421/65 (32%)1.2e−0942/65 (65%)CUB1598 . . . 170440/117 (34%) 7.9e−1570/117 (60%) sushi1715 . . . 177218/65 (28%)4.5e−0640/65 (62%)CUB1776 . . . 188142/116 (36%)   4e−3182/116 (71%) sushi1889 . . . 194419/64 (30%)1.8e−1043/64 (67%)CUB1948 . . . 205345/116 (39%) 1.5e−2977/116 (66%) sushi2061 . . . 211623/64 (36%)7.8e−1347/64 (73%)CUB2120 . . . 222435/116 (30%) 6.4e−2475/116 (65%) sushi2232 . . . 228919/65 (29%)1.4e−0946/65 (71%)CUB2293 . . . 240141/117 (35%) 5.1e−2079/117 (68%) sushi2406 . . . 246420/65 (31%)4.1e−0839/65 (60%)sushi2469 . . . 252619/65 (29%)6.5e−0943/65 (66%)sushi2531 . . . 259117/67 (25%)5.5e−0943/67 (64%)sushi2596 . . . 264920/63 (32%)5.1e−1341/63 (65%)sushi2654 . . . 270722/63 (35%)2.2e−1340/63 (63%)sushi2712 . . . 276518/63 (29%)1.5e−1341/63 (65%)sushi2770 . . . 282820/67 (30%)3.2e−1447/67 (70%)sushi2833 . . . 288619/63 (30%)1.4e−0939/63 (62%)sushi2894 . . . 294717/63 (27%)2.1e−1340/63 (63%)sushi2952 . . . 300618/63 (29%)5.7e−0840/63 (63%)sushi3011 . . . 306619/65 (29%)6.8e−0942/65 (65%)sushi3071 . . . 312424/63 (38%)4.9e−1446/63 (73%)sushi3129 . . . 318218/63 (29%)3.1e−1142/63 (67%)sushi3190 . . . 324420/63 (32%)3.4e−0945/63 (71%)sushi3249 . . . 330418/64 (28%)6.1e−1039/64 (61%)


Example 5

The NOV5 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 5A.

TABLE 5ANOV5 Sequence AnalysisNOV5a, CG50389-04SEQ ID NO: 651897 bpDNA SequenceORF Start: ATG at 51ORF Stop: TAA at 1785GAATTCCGCCCGCCCACGGCGGCGGGGAAATACCTAGGCATGGAAGTGGCATGACAGGGCTCGTGTCCCTGTCATATTTTCCACTCTCCACGAGGTCCTGCGCGCTTCAATCCTGCAGGCAGCCCGGTTTGGGGATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGGGTAGAGACAGCTGTCATAGAATACATGTAAACCTAACTGTTTTTGAAAAACATTGGTGTGACACTTCCATAGGTGGTTTACCAAATTTATCAGATGAGTACAAGCAAATATTACATCTTGGAAAAGATGATAGTCTCACATGTCATCTGCACTTCCCGAAGAGTTGTGTTTTGGGTCCAATAAAGTGGTATAAAGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTAGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCAACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTATCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCCTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCGTTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCACCCAGAAGGTGTCGGCCGTTTCCTCCGGTCCAGCTGCTGCAGCACACACCTTGCTGCCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTCATTCCTACACCTATTTTCTGCTGCAGGATGAGGCTAGGGTTAGCATTCTAGANOV5a, CG50389-04SEQ ID NO: 66578 aaMW at 65576.2kDProtein SequenceMTGLVSLSYFPLSTRSCALQSCRQPGLGMWSLLLCGLSIALPLSVTADGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSISTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILVELEKIEDYTVMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLLQHTPCCRTAGPELGSRRKKCTLTTGNOV5b, CG50389-06SEQ ID NO: 671802 bpDNA SequenceORF Start: ATG at 471ORF Stop: TAA at 1743GGGATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGGTACGTTCCGTGTGTCCTCCGTTCCCAGAGGCTGCCCGAGTCCACAGGCTCCTGGCCTGTCATTGGGAGCCCCCGGGGATCGCGTCAGCCCGAGCGCGGCTCCTTCCCCTCCCCAGACCGCCAGGCGGAGTTCCGCGGAAGAGGAAACAGAGAACCACCAGCTCCACGTGCTCGGATGCTCAGCCCTGAATGTCGCCTTTGAGCTTGGAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTACAGAAAGAGCTGGATATGGAGGAAGTGTCCCTAAAATCATTTATCCAAAAAATCATTCAATTGAAGTACAGCTTGGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGCCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCGTGTGCCACGCTGGAGTGTCCACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTACCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCTTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCATTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCGCCCAGAAGGTGTCGGCCGTTTCCTCCGGTCCAGCTGCCGCAGCACACACCTTGCTACCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTNOV5b, CG50389-06SEQ ID NO: 68424 aaMW at 48256.3kDProtein SequenceMGGLRSLPMCYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHASFREHNLYTVNITFLEVKMEDYGLPFVCHAGVSTAYIILQLPAPDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFLGQAVANVIDENVKLCRPLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILIELEKIEDYTVMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLPQHTPCYRTAGPELGSRRKKCTLTTGNOV5c, 257448648SEQ ID NO: 69891 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGGGTAGAGACAGCTGTCATAGAATACATGTAAACCTAACTGTTTTTGAAAAACATTGGTGTGACACTTCCATAGGTGGTTTACCAAATTTATCAGATGAGTACAAGCAAATATTACATCTTGGAAAAGATGATAGTCTCACATGTCATCTGCACTTCCCGAAGAGTTGTGTTTTGGGTCCAATAAAGTGGTATAAGGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTAGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCCACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACCTCGAGNOV5c, 257448648SEQ ID NO: 70297 aaMW at 33848.1kDProtein SequenceGSDGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSISTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFRAYLENOV5d, CG50389-01SEQ ID NO: 711769 bpDNA SequenceORF Start: ATG at 45ORF Stop: TGA at 477CGCCCGCCCACGGCGGCGGGGAAATACCTAGGCATGGAAGTGGCATGACAGGGCTCGTGTCCCTGTCATATTTTCCACTCTCCACGAGGTCCTGCGCGCTTCAATCCTGCAGGCAGCCCGGTTTGGGGATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAACCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTACAGAAAGAGCTGGATATGGAGGAAGTGTCCCTAAAATCATTTATCCAAAAAATCATTCAATTGAAGTACAGCTTGGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCCACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTATCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCCTGCACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCATTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCGCCCAGAAGGTGTCGGCCGTTTCTCCGGTCCACGTGCCGCAGCACACACCTCTGTACCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTCATTCCTACACCTATTTTCTGCTGCAGGATGAGGCTAGGGTTAGCATTCTACANOV5d, CG50389-01SEQ ID NO: 72144 aaMW at 15977.5kDProtein SequenceMTGLVSLSYFPLSTRSCALQSCRQPGLGMWSLLLCGLSIALPLSVTADGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKTVTRLKGSGSLFWKPGFWNOV5e, CG50389-02SEQ ID NO: 731769 bpDNA SequenceORF Start: ATG at 386ORF Stop: TAG at 1619CGCCCGCCCACGGCGGCGGGGAAATACCTAGGCATGGAAGTGGCATGACAGGGCTCGTGTCCCTGTCATATTTTCCACTCTCCACGAGGTCCTGCGCGCTTCAATCCTGCAGGCAGCCCGGTTTGGGGATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTACAGAAAGAGCTGGATATGGAGGAAGTGTCCCTAAAATCATTTATCCAAAAAATCATTCAATTGAAGTACAGCTTGGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCCACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTATCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCCTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCATTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCGCCCAGAAGGTGTCGGCCGTTTCTCCGGTCCACGTGCCGCAGCACACACCTCTGTACCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTCATTCCTACACCTATTTTCTGCTGCAGGATGAGGCTAGGGTTAGCATTCTAGANOV5e, CG50389-02SEQ ID NO: 74411 aaMW at 46893.7kDProtein SequenceMGGLRSLPMCYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILIELEKIEDYTVMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFLRSTCRSTHLCTAPQAQNNOV5f, CG50389-03SEQ ID NO: 751827 bpDNA SequenceORF Start: ATG at 65ORF Stop: TAA at 1715JGTCATATTTTCCACTCTCCACGAGGTCCTGCGCGCTTCAATCCTGCAGGCAGCCCGGTTTGGGGATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGGGTAGAGACAGCTGTCATAGAATACATGTAAACCTAACTGTTTTTGAAAAACATTGGTGTGACACTTCCATAGGTGGTTTACCAAATTTATCAGATGAGTACAAGCAAATATTACATCTTGGAAAAGATGATAGTCTCACATGTCATCTGCACTTCCCGAAGAGTTGTGTTTTGGGTCCAATAAAGTGGTATAAAGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTAGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCAACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTATCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCCTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCGTTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCACCCAGAAGGTGTCGGCCGTTTCCTCCGGTCCAGCTGCTGCAGCACACACCTTGCTGCCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTCATTCCTACACCTATTTTCTGCTGCAGGATGAGGCTAGGGTTAGCATTCTAGANOV5f, CG50389-03SEQ ID NO: 76550 aaMW at 62620.8kDProtein SequenceMWSLLLCGLSIALPLSVTADGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSISTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILVELEKIEDYTVMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLLQHTPCCRTAGPELGSRRKKCTLTTGNOV5g, CG50389-05SEQ ID NO: 771829 bpDNA SequenceORF Start: ATG at 42ORF Stop: TAA at 1767GGTCCTGCGCGCTTCAATCCTGCAGGCAGCCCGGTTTGGGGATGTGGTCCTTGCAGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAGGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGGGTAGAGACAGCTGTCATAGAATACATGTAAACCTAACTGTTTTTGAAAAACATTGGTGTGACACTTCCATAGGTGGTTTACCAAATTTATCAGATGAGTACAAGCAAATATTACATCTTGGAAAAGATGATAGTCTCACATGTCATCTGCACTTCCCGAAGAGTTGTGTTTTGGGTCCAATAAAGTGGTATAAGGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTACAGAAAGAGCTGGATATGGAGGAAGTGTCCCTAAATCATTTATCCAAAAAAATCATTCAATTGAAGTACAGCTTGGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCCACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTACCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCCTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCATTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCGCCCAGAAGGTGTCGGCCGTTTCCTCCGGTCCAGCTGCCGCAGCACACACCTTGCTACCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTCATNOV5g, CG50389-05SEQ ID NO: 78575 aaMW at 65402.8kDProtein SequenceMWSLQLCGLSIALPLSVTADGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILIELEKIEDYTVMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLPQHTPCYRTAGPELGSRRKKCTLTTGNOV5h, SNP13382464 ofSEQ ID NO: 791897 bpCG50389-04, DNA SequenceORF Start: ATG at 51ORF Stop: TAA at 1785SNP Pos: 1160SNP Change: T to CGAATTCCGCCCGCCCACGGCGGCGGGGAAATACCTAGGCATGGAAGTGGCATGACAGGGCTCGTGTCCCTGTCATATTTTCCACTCTCCACGAGGTCCTGCGCGCTTCAATCCTGCAGGCAGCCCGGTTTGGGGATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGGACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCATAACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAGTCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAJJGGGGGGACTCAGGAGTCTACCAATGTGTTATAAAGGGTAGAGACAGCTGTCATAGAATACATGTAAACCTAACTGTTTTTGAAAAACATTGGTGTGACACTTCCATAGGTGGTTTACCAAATTTATCAGATGAGTACAAGCAAATATTACATCTTGGAAAAGATGATAGTCTCACATGTCATCTGCACTTCCCGAAGAGTTGTGTTTTGGGTCCAATAAAGTGGTATAAAGACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGCAGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAATGGCATCACTGTGAGCATTAGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAATACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATCAGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGGAAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCAACAGCATACATTATATTACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTGTCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTACCGAAGTGCCTTCCATTCTACAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCAGAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAGTTGTTTATATTCGGCAGAGATGAATTCCCTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGCTGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGAAGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCGTTGAGCTGGAGAAAATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGCATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCCACCCAGAAGGTGTCGGCCGTTTCCTCCGGTCCAGCTGCTGCAGCACACACCTTGCTGCCGCACCGCAGGCCCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAAGACTTGCTGGACTGACACCTATGGCTGGAAGATGACTTGTTTTGCTCCATGTCTCCTCATTCCTACACCTATTTTCTGCTGCAGGATGAGGCTAGGGTTAGCATTCTAGANOV5h, SNP13382464 ofSEQ ID NO: 801578 aaMW at 65576.2kDCG50389-04, ProteinSNP Pos: 370SNP Change: Tyr to TyrSequenceMTGLVSLSYFPLSTRSCALQSCRQPGLGMWSLLLCGLSIALPLSVTADGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQATLTHSGKQYEVLNGITVSISTTLIVDCNVTDTKDNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPAPDFPAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKSIILVELEKIEDYTVMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRRPFPPVQLLQHTPCCRTAGPELGSRRKKCTLTTG


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 5B.

TABLE 5BComparison of the NOV5 protein sequences.NOV5a ------------------------------------------------------------NOV5b ------------------------------------------------------------NOV5c -----------------------------------------------GSDGCKDIFMKNENOV5d ------------------------------------------------------------NOV5e ------------------------------------------------------------NOV5f ------------------------------MWSLLLCGLSIALPLSVTADGCKDIFMKNENOV5g MWSLQLCGLSIALPLSVTADGCKDIFMKNEILSASQPFAFNCTFPPITSGEVSVTWYKNSNOV5a ------------------------------------------------------------NOV5b ------------------------------------------------------------NOV5c ILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSNOV5d ------------------------------------------------------------NOV5e ------------------------------------------------------------NOV5f ILSASQPFAFNCTFPPITSGEVSVTWYKNSSKIPVSKIIQSRIHQDETWILFLPMEWGDSNOV5g SKIPVSKIIQSRIHQDETWILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLTVFEKHWCDTNOV5a -------------------------------MTGLVSLS---------------------NOV5b -------------------------------MGGLRSLP---------------------NOV5c GVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFNOV5d -------------------------------MTGLVSLS---------------------NOV5e -------------------------------MGGLRSLP---------------------NOV5f GVYQCVIKGRDSCHRIHVNLTVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFNOV5g SIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPI---------------------NOV5a ---------YFPLSTRSCALQ--SCRQPGLGMWSLLLCGLSIALPLSVTADGCKDIFMKNNOV5b ---------MCYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNNOV5c PKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNNOV5d ---------YFPLSTRSCALQ--SCRQPGLGMWSLLLCGLSIALPLSVTADGCKDIFMKNNOV5e ---------MCYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNNOV5f PKSCVLGPIKWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNNOV5g ---------KWYKDCNEIKGERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNNOV5a EILSASQP--------------------------FAFNCTFPPITSGEVSVTWYKNSSKINOV5b GITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVNOV5c GITVSIST-------------------------TLIVDCNVTDTKDNTNLRCWRVNNTLVNOV5d EILSASQP--------------------------FAFNCTFPPITSGEVSVTWYKNSSKIN0V5e GITVSITERAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVNOV5f GITVSIST-------------------------TLIVDCNVTDTKDNTNLRCWRVNNTLVNOV5g GITVSITEPAGYGGSVPKIIYPKNHSIEVQLGTTLIVDCNVTDTKDNTNLRCWRVNNTLVNOV5a PVSKIIQSRIHQ---------DE---T-WILFLPMEWGDSGVYQCVIKGRDSCHRIHVNLNOV5b DDYYDESKRIREGVETHASFREHNLYTVNITFLEVKMEDYGLPFVCHAGVSTAYIILQLPNOV5c DDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPNOV5d PVSKIIQSRIHQ---------DE---T-WILFLPMEWGDSGVYQCVIK---TVTRLKGSGNOV5e DDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPNOV5f DDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPNOV5g DDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMCHAGVSTAYIILQLPNOV5a TVFEKHWCDTSIGGLPNLSDEYKQILHLGKDDSLTCHLHFPKSCVLGPIKWYKDCNEIKGNOV5b APDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPNOV5c APDFRAYLE---------------------------------------------------NOV5d SLFWKPGFW---------------------------------------------------NOV5e APDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPNOV5f APDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPNOV5g APDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTETIVDGKLYDAYVLYPNOV5a ERFTVLETRLLVSNVSAEDRGNYACQAILTHSGKQYEVLNGITVSISTTLIVDCNVTDTKNOV5b KPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFLGQAVANVIDENVKLCRRLIVNOV5c ------------------------------------------------------------NOV5d ------------------------------------------------------------NOV5e KPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVNOV5f KPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVNOV5g KPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANVIDENVKLCRRLIVNOV5a DNTNLRCWRVNNTLVDDYYDESKRIREGVETHVSFREHNLYTVNITFLEVKMEDYGLPFMNOV5b IVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILIELEKIEDYTVMPESIQYIKQKHGANOV5c ------------------------------------------------------------NOV5d ------------------------------------------------------------NOV5e IVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILIELEKIEDYTVMPESIQYIKQKHGANOV5f IVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILVELEKIEDYTVMPESIQYIKQKHGANOV5g IVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILIELEKIEDYTVMPESIQYIKQKHGANOV5a CHAGVSTAYIILQLPAPDFRAYLIGGLIALVAVAVSVVYIYNIFKIDIVLWYRSAFHSTENOV5b IRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLPQHTPCYRTAGPELGSRRKKCNOV5c ------------------------------------------------------------NOV5d ------------------------------------------------------------NOV5e IRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFLRSTCRSTHLCTAPQAQN--------N0V5f IRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLLQHTPCCRTAGPELGSRRKKCNOV5g IRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLPQHTPCYRTAGPELGSRRKKCNOV5a TIVDGKLYDAYVLYPKPHKESQRHAVDALVLNILPEVLERQCGYKLFIFGRDEFPGQAVANOV5b TLTTG-------------------------------------------------------NOV5c ------------------------------------------------------------NOV5d ------------------------------------------------------------NOV5e ------------------------------------------------------------NOV5f TLTTG-------------------------------------------------------NOV5g TLTTG-------------------------------------------------------NOV5a NVIDENVKLCRRLIVIVVPESLGFGLLKNLSEEQIAVYSALIQDGMKVILVELEKIEDYTNOV5b ------------------------------------------------------------NOV5c ------------------------------------------------------------NOV5d ------------------------------------------------------------NOV5e ------------------------------------------------------------NOV5f ------------------------------------------------------------NOV5g ------------------------------------------------------------NOV5a VMPESIQYIKQKHGAIRWHGDFTEQSQCMKTKFWKTVRYHMPPRRCRPFPPVQLLQHTPCNOV5b ------------------------------------------------------------NOV5c ------------------------------------------------------------NOV5d ------------------------------------------------------------NOV5e ------------------------------------------------------------NOV5f ------------------------------------------------------------NOV5g ------------------------------------------------------------NOV5a CRTAGPELGSRRKKCTLTTGNOV5b --------------------NOV5c --------------------NOV5d --------------------NOV5e --------------------NOV5f --------------------NOV5g --------------------NOV5a (SEQ ID NO: 66)NOV5b (SEQ ID NO: 68)NOV5c (SEQ ID NO: 70)NOV5d (SEQ ID NO: 72)NOV5e (SEQ ID NO: 74)NOV5f (SEQ ID NO: 76)NOV5g (SEQ ID NO: 78)


Further analysis of the NOV5a protein yielded the following properties shown in Table 5C.

TABLE 5CProtein Sequence Properties NOV5aSignalP analysis:Cleavage site between residues 48 and 49PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 14; peak value 7.84PSG score:3.44GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 0.58possible cleavage site: between 40 and 41>>> Seems to have a cleavable signal peptide (1 to 40)ALOM:Klein et al's method for TM region allocationInit position for calculation: 41Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 342-358−9.39PERIPHERALLikelihood = 0.58 (at 473)ALOM score: −9.39 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 20Charge difference: −1.0 C(1.0)-N(2.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 359 to 578)MITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):1.16Hyd Moment(95):1.99G content:4D/E content:1S/T content:11Score: −2.92Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 33 CRQ|PGNUCDISC: discrimination of nuclear localization signalspat4: RRKK (5) at 569pat7: PPRRCRP (4) at 540pat7: PRRCRPF (5) at 541bipartite: nonecontent of basic residues: 10.6%NLS Score: 0.62KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 464LL at 552checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):55.6%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: extracellular, including cell wall>> prediction for CG50389-04 is end (k = 9)


A search of the NOV5a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 5D.

TABLE 5DGeneseq Results for NOV5aNOV5aIdentities/Residues/Similarities forGeneseqProtein/Organism/Length [Patent #,Matchthe MatchedExpectIdentifierDate]ResiduesRegionValueABG69605Human NOV6c protein - Homo1 . . . 578578/578(100%)0.0sapiens, 578 aa. [WO200250277-A2,1 . . . 578578/578(100%)27-JUN-2002]ABG69604Human NOV6b protein - Homo29 . . . 578 550/550(100%)0.0sapiens, 550 aa. [WO200250277-A2,1 . . . 550550/550(100%)27-JUN-2002]AAE16577Human interleukin-1 receptor DNAX29 . . . 547 517/544(95%)0.0designation 6 (IL-1RD6) protein - Homo1 . . . 544519/544(95%)sapiens, 562 aa. [US6326472-B1, 04-DEC-2001]AAE03538Human Interleukin-1 Type 3 receptor29 . . . 547 517/544(95%)0.0(IL-1-3R) - Homo sapiens, 562 aa.1 . . . 544519/544(95%)[US6239268-B1, 29-MAY-2001]AAE06664Human interleukin-1R related protein 229 . . . 547 517/544(95%)0.0(IL-1R6) - Homo sapiens, 562 aa.1 . . . 544519/544(95%)[WO200157219-A2, 09-AUG-2001]


In a BLAST search of public sequence databases, the NOV5a protein was found to have homology to the proteins shown in the BLASTP data in Table 5E.

TABLE 5EPublic BLASTP Results for NOV5aNOV5aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9HB29Interleukin 1 receptor-like 2 precursor29 . . . 578549/575 (95%)0.0(IL-1Rrp2) (Interleukin-1 receptor 1 . . . 575550/575 (95%)related protein 2) (IL1R-rp2) - Homosapiens (Human), 575 aa.CAC60374Sequence 12 from Patent WO015721929 . . . 547517/544 (95%)0.0- unidentified, 562 aa. 1 . . . 544519/544 (95%)Q62929Interleukin 1 receptor-like 2 precursor27 . . . 557358/557 (64%)0.0(Interleukin-1 receptor- related protein 1 . . . 557429/557 (76%)2) (IL1R-rp2) - Rattus norvegicus (Rat),561 aa.Q9ERS7Interleukin 1 receptor-like 2 precursor27 . . . 578364/578 (62%)0.0(IL-1Rrp2) - Mus musculus (Mouse), 1 . . . 570432/578 (73%)574 aa.P14778Interleukin-1 receptor, type I precursor33 . . . 553224/560 (40%)e−101(IL-1R-1) (IL-1R-alpha) (P80) (Antigen 7 . . . 552314/560 (56%)CD121a) - Homo sapiens (Human), 569aa.


PFam analysis predicts that the NOV5a protein contains the domains shown in the Table 5F.

TABLE 5FDomain Analysis of NOV5aIdentities/SimilaritiesPfamNOV5a Matchfor theExpectDomainRegionMatched RegionValueig 63 . . . 12511/64(17%)0.01547/64(73%)ig167 . . . 22513/60(22%)0.941/60(68%)TIR388 . . . 53846/153(30%)2.8e−52136/153(89%)


Example 6.

The NOV6 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 6A.

TABLE 6ANOV6 Sequence AnalysisNOV6a, CG50391-08SEQ ID NO: 812268 bpDNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1565CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAJJACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGAATAGTTTGGGTCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGACTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6a, CG50391-08SEQ ID NO: 82453 aaMW at 50641.5kDProtein SequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRPAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRIVWVTTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6b, CG50391-09SEQ ID NO: 832280 bpDNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1577CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGACCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCACCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6b, CG50391-09SEQ ID NO: 84457 aaMW at 51515.4kDProtein SequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVvRCDTKNKDRCKGSTCNRYQCPAGCLNHKAKIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6c, CG50391-10SEQ ID NO: 852106 bpDNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1403CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAACCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6c, CG50391-10SEQ ID NO: 86399 aaMW at 44463.2kDProtein SequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRPAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6d, CG50391-01SEQ ID NO: 872305 bpDNA SequenceORF Start: ATG at 453ORF Stop: TGA at 1602CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTACAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCCGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAACCTACACTCCAACCTGAAACGGACGAGATGAATGAGGTGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGACCACAAGGCGAGATCTTTGGAACTCTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6d, CG50391-01SEQ ID NO: 88383 aaMW at 43185.7kDProteinSequenceMTNWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6e, CG50391-02SEQ ID NO: 891669 bpDNA SequenceORF Start: ATG at 205ORF Stop: TAG at 1636TCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCCCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTCAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTTCGGTCAACTACATGACCCAAGTCGTCTTATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACACCTGCCCGGCAGCCGCTCGAGCCCTATAGTGTAAACCGATTCGCAGCACACTGGCGCCGTNOV6e, CG50391-02SEQ ID NO: 90477 aaMW at 53587.7kDProtein SequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRATPREDKEEILMLHNKLRGQVQPQASNNEYMTWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSQCPPSYGGSCRNNLCYREETYTPKPETDENNEVETAPIPEENHVWLQPRVMRPTKPKKTSSVNYMTQVVLCDTKMKDRCKGSTCNRYQCPAGCLMIKAKIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNTYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYTCPAAARALNOV6f, CG50391-03SEQ ID NO: 912400 bpDNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1697CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCCGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6f, CG50391-03SEQ ID NO: 92497 aaMW at 55928.2kDProtein SequenceMSCVLGGVIPLGLLFLVRGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITPNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6g, CG50391-04SEQ ID NO: 932403 bpDNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1700CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGCGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCCGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6g, CG50391-04SEQ ID NO: 94498 aaMW at 56114.4kDProtein SequenceMSCVLGGVIPLGLLFLVRGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6h, CG50391-05SEQ ID NO: 952412 bpDNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1709CTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCCGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCCATGTGCACGCACTACACACAGGTAACTCAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGAGGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6h, CG50391-05SEQ ID NO: 96501 aaMW at 56412.8kDProtein SequenceMSCVLGGVIPLGLLFLVRGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQVTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKRGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6i, CG50391-06SEQ ID NO: 971425 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCGGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCTGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGNOV6i, CG50391-06SEQ ID NO: 98475 aaMW at 53743.6kDProtein SequenceYLLPNVTLLEELLSKYQHNESHSRVRPAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6j, CG50391-07SEQ ID NO: 992272 bpDNA SequenceORF Start: ATG at 216ORF Stop: TGA at 1707CCCAGGGCGTCTCCGGCTGCTCCCATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTGCTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCNAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCTATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCTGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCTGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCCCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAAGGCNOV6j, CG50391-07SEQ ID NO: 100497 aaMW at 55905.2kDProtein SequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6k, CG50391-11SEQ ID NO: 1011612 bpDNA SequenceORF Start: ATG at 31ORF Stop: TGA at 1522GAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGGTATCGCTCTCCGGGGTTCCATGTGCAGTCCTGGTATGACGAGGTGAAGGACTACACCTACCCCTACCCGAGCGAGTGCAACCCCTGGTGTCCAGAGAGGTGCTCGGGGCCCATGTGCACGCACTACACACAGATAGTTTGGGCCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGGAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAACTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATAGGAGNOV6k, CG50391-11SEQ ID NO: 102497 aaMW at 55847.2kDProtein SequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVGTAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGTLFYESSSSICRAATHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6l, SNP13376336 ofSEQ ID NO: 1032268 bpCG50391-08, DNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1565SNP Pos: 393SNP Change: A to GCTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCGCAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGAATAGTTTGGGTCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGACGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6l, SNP13376336 ofSEQ ID NO: 104453 aaMW at 50660.5kDCG50391-08, ProteinSNP Pos: 63SNP Change: His to ArgMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLRNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRIVWVTTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6m, SNP13376335 ofSEQ ID NO: 1052268 bpCG50391-08, DNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1565SNP Pos: 477SNP Change: C to TCTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGTAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGAATAGTTTGGGTCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6m, SNP13376335 ofSEQ ID NO: 106453 aaMW at 50669.5kDCG50391-08, ProteinSNP Pos: 91SNP Change: Ala to ValSequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAVAWASQCIWEHGPTSLLVSIGQNLGAHWGRIVWVTTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITPNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6n, SNP13376334 ofSEQ ID NO: 1072268 bpCG50391-08, DNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1565SNP Pos: 798SNP Change: C to TCTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGAATAGTTTGGGTCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCTAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6n, SNP13376334 ofSEQ ID NO: 108453 aaMW at 50657.5kDCG50391-08, ProteinSNP Pos: 198SNP Change: Pro to LeuSequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRIVWVTTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTLKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6o, SNP13376333 ofSEQ ID NO: 1092268 bpCG50391-08, DNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1565SNP Pos: 816SNP Change: A to GCTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGAATAGTTTGGGTCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGGGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCA~ATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCCGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGGACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6o, SNP13376333 ofSEQ ID NO: 110453 aaMW at 50569.4kDCG50391-08, ProteinSNP Pos: 204SNP Change: Glu to GlySequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRPAIPREDKEEILMLHNKLRGQVQPQASNNEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRIVWVTTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDGMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6p, SNP13382488 ofSEQ ID NO: 1112268 bpCG50391-08, DNA SequenceORF Start: ATG at 206ORF Stop: TGA at 1565SNP Pos: 1402SNP Change: C to TCTCTGACTGCTCCTATTGAGCTGTCTGCTCGCTGTGCCCGCTGTGCCTGCTGTGCCCGCGCTGTCGCCGCTGCTACCGCGTCTACTGGACGCGGGAGACGCCAGCGAGCTGGTGATTGGAGCCCTGCGGAGAGCTCAAGCGCCCAGCTCTGCCCGAGGAGCCCAGGCTGCCCCGTGAGTCCCATAGTTGCTGCAGGAGTGGAGCCATGAGCTGCGTCCTGGGTGGTGTCATCCCCTTGGGGCTGCTGTTCCTGGTCTGCGGATCCCAAGGCTACCTCCTGCCCAACGTCACTCTCTTAGAGGAGCTGCTCAGCAAATACCAGCACAACGAGTCTCACTCCCGGGTCCGCAGAGCCATCCCCAGGGAGGACAAGGAGGAGATCCTCATGCTGCACAACAAGCTTCGGGGCCAGGTGCAGCCTCAGGCCTCCAACATGGAGTACATGACCTGGGATGACGAACTGGAGAAGTCTGCTGCAGCGTGGGCCAGTCAGTGCATCTGGGAGCACGGGCCCACCAGTCTGCTGGTGTCCATCGGGCAGAACCTGGGCGCTCACTGGGGCAGAATAGTTTGGGTCACCACCAACAAGATCGGTTGTGCTGTGAACACCTGCCGGAAGATGACTGTCTGGGGAGAAGTTTGGGAGAACGCGGTCTACTTTGTCTGCAATTATTCTCCAAAGGGGAACTGGATTGGAGAAGCCCCCTACAAGAATGGCCGGCCCTGCTCTGAGTGCCCACCCAGCTATGGAGGCAGCTGCAGGAACAACTTGTGTTACCGAGAAGAAACCTACACTCCAAAACCTGAAACGGACGAGATGAATGAGGTGGAAACGGCTCCCATTCCTGAAGAAAACCATGTTTGGCTCCAACCGAGGGTGATGAGACCCACCAAGCCCAAGAAAACCTCTGCGGTCAACTACATGACCCAAGTCGTCAGATGTGACACCAAGATGAAGGACAGGTGCAAAGGGTCCACGTGTAACAGGTACCAGTGCCCAGCAGGCTGCCTGAACCACAAGGCGAAGATCTTTGGAAGTCTGTTCTATGAAAGCTCGTCTAGCATATGCCGCGCCGCCATCCACTACGGGATCCTGGATGACAAGGGAGGCCTGGTGGATATCACCAGGAACGGGAAGGTCCCCTTCTTCGTGAAGTCTGAGAGACACGGCGTGCAGTCCCTCAGCAAATACAAACCTTCCAGCTCATTCATGGTGTCAAAAGTGAAAGTGCAGGATTTGGACTGCTACACGACCGTTGCTCAGCTGTGCCCGTTTGAAAAGCCAGCAACTCACTGCCCAAGAATCCATTGTCCGGCACACTGCAAAGACGAACCTTCCTACTGGGCTCCGGTGTTTGGAACCAACATCTATGCAGATACCTCAAGCATCTGCAAGACAGCTGTGCACGCGGGAGTCATCAGCAACGAGAGTGGGGGTGACGTGGACGTGATGCCCGTGGATAAAAAGAAGACCTACGTGGGCTCGCTCAGGAATGGAGTTCAGTCTGAAAGCCTGGGGACTCCTCGGGATGGAAAGGCCTTCCGGATCTTTGCTGTCAGGCAGTGAATTTCCAGCACCAGGGGAGAAGGGGCGTCTTCAGGAGGGCTTCGGGGTTTTGCTTTTATTTTTATTTTGTCATTGCGGGGTATATGGAGAGTCAGGAAACTTCCTTTGACTGATGTTCAGTGTCCATCACTTTGTGGCCTGTGGGTGAGGTGACATCTCATCCCCTCACTGAAGCAACAGCATCCCAAGGTGCTCAGCCGCACTCCCTGGTGCCTGATCCTGCTGGGGCCCGGGGGTCTCCATCTGGACGTCCTCTCTCCTTTAGAGATCTGAGCTGTCTCTTAAAGGGGACAGTTGCCCAAAATGTTCCTTGCTATGTGTTCTTCTGTTGGTGGAGGAAGTTGATTTCAACCTCCCTGCCAAAAGAACAAACCATTTGAAGCTCACAATTGTGAAGCATTCACGGCGTCGGAAGAGGCCTTTTGAGCAAGCGCCAATGAGTTTCAGGAATGAAGTAGAAGGTAGTTATTTAAAAATAAAAAACACAGTCCGTCCCTACCAATAGAGGAAAATGGTTTTAATGTTTGCTGGTCAGACAGACAAATGGGCTAGAGTAAGAGGGCTGCGGGTATGAGAGACCCCGGCTCCGCCCTGGCACGTGTCCTTGCTGGCGGCCCGCCACAGGCCCCCTTCAATGGCCGCATTCAGGATGGCTCTATACACAGCAGTGCTGGTTTATGTAAAGTTCAGCAGTCACTTCANOV6p, SNP13382488 ofSEQ ID NO: 112453 aaMW at 50641.5kDCG50391-08, ProteinSNP Pos: 399SNP Change: Ala to AlaSequenceMSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRPAIPREDKEEILMLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRIVWVTTNKIGCAVNTCRKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKAKIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLENGVQSESLGTPPDGKAFRIFAVRQ


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 6B.

TABLE 6BComparison of the NOV6 protein sequences.NOV6a ----------------------------------------MSCVLGGVIPLGLLFLVCGSNOV6b ----------------------------------------MSCVLGGVIPLGLLFLVCGSNOV6c ----------------------------------------MSCVLGGVIPLGLLFLVCGSNOV6d ------------------------------------------------------------NOV6e ----------------------------------------MSCVLGGVIPLGLLFLVCGSNOV6f ----------------------------------------MSCVLGGVIPLGLLFLVRGSNOVGg ----------------------------------------MSCVLGGVIPLGLLFLVRGSNOV6h ----------------------------------------MSCVLGGVIPLGLLFLVRGSNOV6i ----------------------YLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILNOVGj ----------------------------------------MSCVLGGVIPLGLLFLVCGSNOVGk MSCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILNOV6a QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQAS-----NOV6b QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQAS-----NOV6c QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQAS-----NOV6d ------------------------------------------------------M-----NOV6e QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMNOV6f QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMNOV6g QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQAS-----NOV6h QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMNOV6i MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLG-----NOV6j QGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEILMLHNKLRGQVQPQASNMEYMNOV6k MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLG-----NOV6a ------------------------------------------N--MEYMTWDDELEKSAANOV6b -----------------------------------NMEYMYRSPGFHVQSWYDEVKDYTYNOV6c ------------------------------------------N--MEYMTWDDELEKSAANOV6d -----------------------------------TNWGRYRSPGFHVQSWYDEVKDYTYNOV6e TWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYNOV6f TWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYNOV6g ------------------------------------------N--MEYMTWDDELEKSAANOVGh TWDDELEKSAAAWASQCIWEHGPTGLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYNOV6i -----------------------------------AHWGRYRSPGFHVQSWYDEVKDYTYNOV6j TWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGRYRSPGFHVQSWYDEVKDYTYNOV6k -----------------------------------AHWGRYRSPGFHVQSWYDEVKDYTYNOV6a AWASQC-----------IWEHG--PTSLLVSIGQNLGAHWGRIV----------------NOV6b PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6c AWASQC-----------IWEHG--PTSLLVSIGQNLGAHWG-------------------NOV6d PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6e PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6f PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6g AWASQC-----------IWEHG--PTSLLVSIGQNLGAHWGRRYRSPGFHVQSWYDEVKDNOV6h PYPSECNPWCPERCSGPMCTHYTQVTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6i PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6j PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6k PYPSECNPWCPERCSGPMCTHY---TQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6a -----------------------------WVTTNKIGCAVNTCRKMTVWGEVWENAVYFVNOV6b CNYSPK------------------------------------------------------NOV6c ------------------------------------------------------------NOV6d CNYSPK------------------------------------------------------NOV6e CNYSPK------------------------------------------------------NOV6f CNYSPK------------------------------------------------------NOV6g YTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRKMTVWGEVWENAVYFVNOVGh CNYSPKR-----------------------------------------------------NOV6i CNYSPK------------------------------------------------------NOV6j CNYSPK------------------------------------------------------NOV6k CNYSPK------------------------------------------------------NOV6a CNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6b ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6c -----RGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6d ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6e ------GNWIGEAPYKNGRPCSQCPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6f ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6g CNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6h ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6i ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6j ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVETAPINOV6k ------GNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETYTPKPETDEMNEVGTAPINOV6a PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6b PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6c PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6d PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6e PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6f PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6g PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6h PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6i PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6j PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6k PEENHVWLQPRVMRPTKPKXTSAVNYMTQVVRCDTKMKDRCKGSTCNRYQCPAGCLNHKANOV6a KIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6b KIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6c KIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSL-------NOV6d KIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6e KIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6f KIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6g KIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6h KIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6i KIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6j KIFGSLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6k KIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFVKSERHGVQSLSKYKPSSNOV6a SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6b SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6c --------VQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6d SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6e SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6f SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6g SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6h SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6i SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6j SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6k SFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDEPSYWAPVFGTNIYADTSNOV6a SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6b SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6c SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6d SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6e SICKTAVHAGVISNESGGDVDVMPVDKKKTYTCPAAARAL--------------------NOV6f SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6g SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6h SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6i SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6j SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6k SICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSESLGTPRDGKAFRIFAVRQNOV6a (SEQ ID NO: 82)NOV6b (SEQ ID NO: 84)NOV6c (SEQ ID NO: 86)NOV6d (SEQ ID NO: 88)NOV6e (SEQ ID NO: 90)NOV6f (SEQ ID NO: 92)NOV6g (SEQ ID NO: 94)NOV6h (SEQ ID NO: 96)NOV6i (SEQ ID NO: 98)NOV6j (SEQ ID NO: 100)NOV6k (SEQ ID NO: 102)


Further analysis of the NOV6a protein yielded the following properties shown in Table 6C.

TABLE 6CProtein Sequence Properties NOV6aSignalP analysis:Cleavage site between residues 23 and 24PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 31; peak value 10.73PSG score:6.33GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.72possible cleavage site: between 22 and 23>>> Seems to have a cleavable signal peptide (1 to 22)ALOM:Klein et al's method for TM region allocationInit position for calculation: 23Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 7.37 (at 106)ALOM score: 7.37 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 11Charge difference: −1.0 C(0.0)-N(1.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment (75):0.40Hyd Moment (95):3.00G content:5D/E content:1S/T content:3Score: −7.63Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: KPKK (4) at 230pat7: PTKPKKT (4) at 228pat7: PVDKKKT (4) at 417bipartite: nonecontent of basic residues: 11.9%NLS Score: 0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):52.2%: nuclear21.7%: extracellular, including cell wall17.4%: mitochondrial 4.3%: vacuolar 4.3%: cytoplasmic>> prediction for CG50391-08 is nuc (k = 23)


A search of the NOV6a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 6D.

TABLE 6DGeneseq Results for NOV6aNOV6aIdentities/Residues/Similarities forGeneseqProtein/Organism/Length [Patent #,Matchthe MatchedExpectIdentifierDate]ResiduesRegionValueAAU83619Human PRO protein, Seq ID No 56 -1 . . . 453452/497 (90%)0.0Homo sapiens, 497 aa.1 . . . 497452/497 (90%)[WO200208288-A2, 31-JAN-2002]AAO21721Human secreted protein SEQ ID No 63 -1 . . . 453452/497 (90%)0.0Homo sapiens, 497 aa.1 . . . 497452/497 (90%)[WO200238602-A2, 16-MAY-2002]AAB74446Human protease-inhibitor like protein -1 . . . 453452/497 (90%)0.0Homo sapiens, 497 aa.1 . . . 497452/497 (90%)[WO200121651-A2, 29-MAR-2001]AAE10618Human novel trypsin inhibitor-like1 . . . 453451/498 (90%)0.0protein, NOV-4d - Homo sapiens, 4981 . . . 498451/498 (90%)aa. [WO200162928-A2, 30-AUG-2001]AAE10616Human novel trypsin inhibitor-like1 . . . 453450/497 (90%)0.0protein, NOV-4b - Homo sapiens, 4971 . . . 497450/497 (90%)aa. [WO200162928-A2, 30-AUG-2001]


In a BLAST search of public sequence databases, the NOV6a protein was found to have homology to the proteins shown in the BLASTP data in Table 6E.

TABLE 6EPublic BLASTP Results for NOV6aNOV6aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9H0B8Hypothetical protein - Homo1 . . . 453451/497 (90%)0.0sapiens (Human), 497 aa.1 . . . 497452/497 (90%)CAC69820Sequence 20 from Patent1 . . . 453451/498 (90%)0.0WO0162928 - Homo sapiens1 . . . 498451/498 (90%)(Human), 498 aa.CAC69818Sequence 16 from Patent1 . . . 453450/497 (90%)0.0WO0162928 - Homo sapiens1 . . . 497450/497 (90%)(Human), 497 aa.CAC69821Sequence 22 from Patent1 . . . 453450/501 (89%)0.0WO0162928 - Homo sapiens1 . . . 501450/501 (89%)(Human), 501 aa.CAC69817Sequence 14 from Patent81 . . . 453 339/373 (90%)0.0WO0162928 - Homo sapiens16 . . . 383 348/373 (92%)(Human), 383 aa.


PFam analysis predicts that the NOV6a protein contains the domains shown in the Table 6F.

TABLE 6FDomain Analysis of NOV6aIdentities/SimilaritiesPfamNOV6a Matchfor theExpectDomainRegionMatched RegionValueSCP 60 . . . 15545/167 (27%)7.4e−2376/167 (46%)LCCL244 . . . 33547/104 (45%)6.1e−4984/104 (81%)LCCL345 . . . 44455/104 (53%)1.6e−5189/104 (86%)


Example 7

The NOV7 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 7A.

TABLE 7ANOV7 Sequence AnalysisNOV7a, CG50426-17SEQ ID NO: 1138160 bpDNA SequenceORF Start: ATG at 122ORF Stop: TAA at 8108GGGAGTTGGAACCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTNOV7a, CG50426-17SEQ ID NO: 1142662 aaMW at 295881.9kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKIWVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIEAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRPASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7b, CG50426-21SEQ ID NO: 1159852 bpDNA SequenceORF Start: ATG at 210ORF Stop: TAA at 8505CCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGCTCTGAAGGTCTGAAAGATTCTGCTGCAACTAAAGCTCTGAAGATTCTGCTACAACTATGACATCCATTTTCTCCCACTTCAGACAGGATGAATACAAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTCCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGCAATGCATCTGCTCGGACTCAATTGGCAACTCCAGCCTGCAGATGGGCACACCTTTAACAATGGGATAAGGACCGGCTTACCAGGAAACGATGATGTGGCAACAATGCCATCTGGAGGCAAAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCATGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGGGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAACTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTGGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACAGATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7b, CG50426-21SEQ ID NO: 1162765 aaMW at 306806.1kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGIVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIAMHLLGLNWQLQPADGHTFNNGIRTGLPGNDDVATMPSGGKVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEIKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7c, CG50426-15SEQ ID NO: 1172478 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATNOV7c, CG50426-15SEQ ID NO: 118826 aaMW at 89833.6kDProtein SequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNNOV7d, 306276924SEQ ID NO: 1192497 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATCTCGAGGGCNOV7d, 306276924SEQ ID NO: 120832 aaMW at 90551.4kDProtein SequenceTKLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNLEGNOV7e, 306276936SEQ ID NO: 1212470 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTCTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATCTCGAGGGCNOV7e, 306276936SEQ ID NO: 122823 aaMW at 89649.5kDProtein SequenceTKLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLEAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMXTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITNTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNLEGNOV7f, 308530526SEQ ID NO: 1232470 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACAGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCATGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATCTCGAGGGCNOV7f, 308530526SEQ ID NO: 124823 aaMW at 89649.5kDProtein SequenceTKLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNLEGNOV7g, 308530589SEQ ID NO: 1252284 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATCTCGAGGGCNOV7g, 308530589SEQ ID NO: 126761 aaMW at 83010.1kDProtein SequenceTKLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNLEGNOV7h, CG50426-01SEQ ID NO: 1271431 bpDNA SequenceORF Start: ATG at 69ORF Stop: TAG at 1212AAAAAAGGCGGGGGGTGGACTTAGCAGTGTAATTTGAGACCGGTGGTAAGGATTGGAGCGAGCTAGAGATGCTGCACGCTGCTAACAAGGGAAGGAAGCCTTCAGCTGAGGCAGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATGGGACCATCTAGTTGCAGAAAAACAAGCTCAGGGCGCCCACTGATTTGACATTATGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAANTGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGACCTTCCTGCCTTGACTTTCTGCAAGACAATCATTAATAAAGCTGCTCTGTAAATACTAAAAAAAAAACANOV7h, CG50426-01SEQ ID NO: 128381 aaMW at 42017.0kDProtein SequenceMLHAANKGRKPSAEAGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLHACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDGTINOV7i, CG50426-02SEQ ID NO: 1291447 bpDNA SequenceORF Start: ATG at 277ORF Stop: at 1447GCTCCAAAGCGAGCTGGGACCGAAGACTCTAGGCTAAGTTATCTATGTAGATGGTGTCAGGGAGCGAAGCTACTGACCGAGCTGCTGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGcTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCNOV7i, CG50426-02SEQ ID NO: 130390 aaMW at 42705.6kDProtein SequenceMDVKDRPHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLPACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILNOV7j, CG50426-03SEQ ID NO: 1314888 bpDNA SequenceORF Start: ATG at 247ORF Stop: TGA at 1840TACTCACTATAGGGCTCGAGCGGCCGCCCGGGCAGGTCCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGCTCTGAAGGTCTGAAAGATTCTGCTGCAACTAAAGCTCTGAAGATTCTGCTACAACTATGACATCCATTTTCTCCCACTTCAGACAGGATGAATACAAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTCCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATAGCTGAGGTAGCCGGCTCTGGCCTCGGCTAGCCCAGAAAGGGGCTCCCACAGTGCAGCAGCGGGCTGAAGGGCTCCTCAAGCGTGGCCAGAGTGGGCGCTGAGGCCGAGGAGGCACCGAGAGCGAGCGAGGGCTGCAAGGTCTGCCAGCACGCTGTCACTTCTCACTATTTTGATTGGTATTGTGTCCGGAATTGGTGGGTTCTTGGTCTCACTGACTTCAAGAATGAAGCCACGGACCCTCGCGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCGCTCCAAAGCGAGCTGGGACCGAAGACTCTAGGCTAAGTTATCTATGTAGATGGTGTCAGGGAGCGAAGCTACTGACCGAGCTGCTGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCNOV7j, CG50426-03SEQ ID NO: 132531 aaMW at 58938.0kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGTLHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSNOV7k, CG50426-04SEQ ID NO: 1331605 bpDNA SequenceORF Start: ATG at 435ORF Stop: at 1605AGCTGAGGTAGCCGGCTCTGGCCTCGGCTAGCCCAGAAAGGGGCTCCCACAGTGCAGCAGCGGGCTGAAGGGCTCCTCAAGCGTGGCCAGAGTGGGCGCTGAGGCCGAGGAGGCACCGAGAGCGAGCGAGGGCTGCAAGGTCTGCCAGCACGCTGTCACTTCTCACTATTTTGATTGGTATTGTGTCCGGAATTGGTGGGTTCTTGGTCTCACTGACTTCAAGAATGAAGCCACGGACCCTCGCGTTACATCCAGCTTTTTAATTGCCTAACCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCNOV7k, CG50426-04SEQ ID NO: 134390 aaMW at 42705.6kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILNOV7l, CG50426-05SEQ ID NO: 1359668 bpDNA SequenceORF Start: ATG at 122ORF Stop: TAA at 8321GGGAGTTGGAACCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGCGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAAGTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTGGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACAGATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7l, CG50426-05SEQ ID NO: 1362733 aaMW at 303423.3kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSPRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLPACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVITDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPTVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVFNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7m, CG50426-06SEQ ID NO: 1371920 bpDNA SequenceORF Start: at 180ORF Stop: at 1920CCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGCTCTGAAGGTCTGAAAGATTCTGCTGCAACTAAAGCTCTGAAGATTCTGCTACAACTATGACATCCATTTTCTCCCACTTCAGACAGGATGAATACAAGCTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTCCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGGTATGTGCCGCCACTTCCCTGNOV7m, CG50426-06SEQ ID NO: 138580 aaMW at 64132.9kDProtein SequenceKHQHSAISGIMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKGMCRHFPNOV7n, CG50426-07SEQ ID NO: 1391447 bpDNA SequenceORF Start: at 247ORF Stop: at 1447GCTCCAAAGCGAGCTGGGACCGAAGACTCTAGGCTAAGTTATCTATGTAGATGGTGTCAGGGAGCGAAGCTACTGACCGAGCTGCTGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAQAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCNOV7n, CG50426-07SEQ ID NO: 140400 aaMW at 43764.8kDProtein SequenceKHQHSAISGIMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRNHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLEACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILNOV7o, CG50426-08SEQ ID NO: 1419823 bpDNA SequenceORF Start: ATG at 277ORF Stop: TAA at 8476GCTCCAAAGCGAGCTGGGACCGAAGACTCTAGGCTAAGTTATCTATGTAGATGGTGTCAGGGAGCGAAGCTACTGACCGAGCTGCTGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGGGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAAGTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTGGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACACATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7o, CG50426-08SEQ ID NO: 1422733 aaMW at 303423.3kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRVSKNKPXTLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRIAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNov7p, CG50426-09SEQ ID NO: 1431920 bpDNA SequenceORF Start: at 180ORF Stop: at 1920CCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGCTCTGAAGGTCTGAAAGATTCTGCTGCAACTAAAGCTCTGAAGATTCTGCTACAACTATGACATCCATTTTCTCCCACTTCAGACAGGATGAATACAAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTCCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCANOV7p, CG50426-09SEQ ID NO: 144580 aaMW at 63961.7kDProtein SequenceKHQHSAISGIMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSPNHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHTSQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCNOV7q, CG50426-10SEQ ID NO: 1459756 bpDNA SequenceORF Start: ATG at 210ORF Stop: TAA at 8409CCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGCTCTGAAGGTCTGAAAGATTCTGCTGCAACTAAAGCTCTGAAGATTCTGCTACAACTATGACATCCATTTTCTCCCACTTCAGACAGGATGAATACAAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCAATGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAACACCACATCCTCAATGTTAAAAGTGGAAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTAATATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACAAGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGGGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAAGTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTGGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACAGATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7q, CG50426-10SEQ ID NO: 1462733 aaMW at 303423.3kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYTRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCNGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIENGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLTANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSTMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQITTLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYPYIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVTGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFATKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7r, CG50426-11SEQ ID NO: 1479729 bpDNA SequenceORF Start: ATG at 210ORF Stop: TAA at 8382CCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGCTCTGAAGGTCTGAAAGATTCTGCTGCAACTAAAGCTCTGAAGATTCTGCTACAACTATGACATCCATTTTCTCCCACTTCAGACAGGATGAATACAAGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTCCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCATGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGGGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAAGTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTGGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACAGATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7r, CG50426-11SEQ ID NO: 1482724 aaMW at 302521.3kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGpLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7s, CG50426-12SEQ ID NO: 1499826 bpDNA SequenceORF Start: ATG at 280ORF Stop: TAA at 8479TTTAAATCCTCATACCTTAAAGGAGATGTGTATATAAGGGAGTTGGAACCAGCATTAGATGAGTTGACAAAAATGCAGTTTCAGTTCTAGAGGTCTGGGAAGTCCAAGAACAAGGTGCTGGCAGATTGGATTCCCCGTGAGGGCTTTCTTCCTGGCTTGAAGTTGGCTGCTTTCCTGCTGAGACTTCTCATGGCAGAGACTGAGGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTAACTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCAAAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGGGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAAGTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTGGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACAGATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7s, CG50426-12SEQ ID NO: 1502733 aaMW at 303423.3kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWNGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAYNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRITNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVENFGKFGVTYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRPFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7t, CG50426-13SEQ ID NO: 1511605 bpDNA SequenceORF Start: at 405ORF Stop: at 1605AGCTGAGGTAGCCGGCTCTGGCCTCGGCTAGCCCAGAAAGGGGCTCCCACAGTGCAGCAGCGGGCTGAAGGGCTCCTCAAGCGTGGCCAGAGTGGGCGCTGAGGCCGAGGAGGCACCGAGAGCGAGCGAGGGCTGCAAGGTCTGCCAGCACGCTGTCACTTCTCACTATTTTGATTGGTATTGTGTCCGGAATTGGTGGGTTCTTGGTCTCACTGACTTCAAGAATGAAGCCACGGACCCTCGCGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCNOV7t, CG50426-13SEQ ID NO: 152400 aaMW at 43764.8kDProtein SequenceKHQHSAISGIMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPUDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRINTFSPAFKLKKPSKYCSWKCAALSAIAAALLLAILNOV7u, CG50426-14SEQ ID NO: 1539823 bpDNA SequenceORF Start: ATG at 277ORF Stop: TAA at 8476GCTCCAAAGCGAGCTGGGACCGAAGACTCTAGGCTAAGTTATCTATGTAGATGGTGTCAGGGAGCGAAGCTACTGACCGAGCTGCTGTTACATCCAGCTTTTTAATTGCCTAAGCGGTCTGGGGCTTGCTTCGTCATTTGGCTTTGCTGTGGAGCACTCCTGTAAAGCCAGCTGAATTGTACATCGAAGATCCACCCTTTTGGTGGCAAAGTGACAAGTGCCAAAACTCAGGCCTGACTTTTCTGAAAACATCAGCATTCTGCCATATCTGGAATAATGGATGTAAAGGACCGGCGACACCGCTCTTTGACCAGAGGACGCTGTGGCAAAGAGTGTCGCTACACAAGCTCCTCTCTGGACAGTGAGGACTGCCGGGTGCCCACACAGAAATCCTACAGCTCCAGTGAGACTCTGAAGGCCTATGACCATGACAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGAACCAACTTCACCCTTGCCGAACTGGGCATCTGTGAGCCCTCCCCACACCGAAGCGGCTACTGCTCCGACATGGGGATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACCTACATCCTCGCCTAGTCTCCTCCCATCTGCTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGCCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCATAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGGCTGGGCTGCTTTAGGAGACCAAGTGGCAAGAAAGCTCACATTTTTTGAGTTCAAATGCTACTGTCCAAGCGAGAAGTCCCTCATCCTGAAGTAGACTAAAGCCCGGCTGAAAATTCCGAGGAAAACAAAACAAACGAATGAATGAACAGACACACACAATGTTCCAAGTTCCCCTAAAATATGACCCACTTGTTCTGGGTCTACGCAGAAAAGAGACGCAAAGTGTCCAAAAGGAACAAAAGAACAAAAACGAATAAGCAAAGAAGAAAACAAACAAAAACAAAACAAAACAAACACACGGACCGATAAACAAAGAAGCGAAGATAAGAAAGAAGGCCTCATATCCAATTACCTCACTCATTCACATGTGAGCGACACGCAGACATCCGCGAGGGCCAGCGTCACCAGACCAGCTGCGGGACAAACCACTCAGACTGCTTGTAGGACAAATACTTCTGACATTTTCGTTTAAGCAAATACAGGTGCATTTAAAACACGACTTTGGGGGTGATTTGTGTGTAGCGCCTGGGGAGGGGGGATAAAAGAGGAGGAGTGAGCACTGGAAATACTTTTTAAAGAAAAAAAAACATGAGGGAATAAAAGAAATTCCTATCAAAAATCAAAGTGAAATAATACCATCCAGCACTTAACTCTCAGGTCCCAACTAAGTCTCGCCTGAGCTAATTTATTTGAGCGCAGAGTGTAAAATTTAATTCAAAATGGTGGCTATAATCACTACAGATAAATTTCATACTCTTTTGTCTTTGGAGATTCCATTGTGGACAGTAATACGCAGTTACAGGGTGTAGTCTGTTTAGATTCCGTAGTTCGTGGGTATCAGTTTCGGTAGAGGTGCAGCATCGTGACACTTTTGCTAACAGGTACCACTTCTGATCACCCTGTACATACATGAGCCGAAAGGCACAATCACTGTTTCAGATTTAAAATTATTAGTGTGTTTGTTTGGTCCAGAAACTGAGACAATCACATGACAGTCACCACGAGGAGAGAAAATTTAAAAAATAAAAATAAAAACAAAAAAAATTTTAAAAATTAAAAAAACAAAAATAAAGTCTAATAAGAACTTTGGTACAGGAACTTTTTTGTAATATACATGTATGAATTGTTCATCGAGTTTTTATATTAATTTTAATTTGCTGCTAAGCAAAGACTAGGGACAGGCAAAGATAATTTATGGCAAAGTGTTTAAATTGTTTATACATAAATAAAGTCTCTAAAACTCCTGTGNOV7u, CG50426-14SEQ ID NO: 1542733 aaMW at 303423.3kDProtein SequenceMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWNGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAYNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRITNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVENFGKFGVTYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRPFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7v, CG50426-16SEQ ID NO: 1552478 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCATGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGTCGCCGGAGCATTTCTTGTCCCAGCTGCAACGGCCTCGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATNOV7v, CG50426-16SEQ ID NO: 156826 aaMW at 89909.7kDProtein SequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPNNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNNOV7w, CG50426-18SEQ ID NO: 15757 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAANOV7w, CG50426-18SEQ ID NO: 15819 aaMW at 2029.2kDProtein SequenceDTGLCSCDPNWMGPDCSVENOV7x, CG50426-19SEQ ID NO: 15954 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGNOV7x, CG50426-19SEQ ID NO: 16018 aaMW at 1963.2kDProtein SequenceDRIKLLAGKDSTHIIPGENOV7y, CG50426-20SEQ ID NO: 16160 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCNOV7y, CG50426-20SEQ ID NO: 16220 aaMW at 2143.3kDProtein SequenceSAGYKGEHCEEVDCLDPTCSNOV7z, CG50426-22SEQ ID NO: 163540 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGACCTTCCTGCCTTGACTTTCTGCAAGACAATCATTAATAAAGCTGCTCTGNOV7z, CG50426-22SEQ ID NO: 164180 aaMW at 20167.7kDProtein SequenceNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKENVSFNTVVLDDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKDLPALTFCKTIINKAALNOV7aa, CG50426-23SEQ ID NO: 1652497 bpDNA SequenceORF Start: at 11ORF Stop: at 2489CACCAAGCTTAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATCTCGAGGGCNOV7aa, CG50426-23SEQ ID NO: 166826 aaMW at 89909.7kDProtein SequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPNNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNNOV7ab, CG50426-24SEQ ID NO: 1678575 bpDNA SequenceORF Start: ATG at 199ORF Stop: TAA at 8476TAAAGTACCTGTCATCTTGACAAGTGGCGGAGCGGAGGAGTCAAGGATTATAAATGATCACAGCCAGGTCCAGCTCGCCCCGTGATTGGGCTCTCCCGCGATCTGCACCGGGGGAAGCGCATGAGAGGCCAATGAGACTTGAACCCTGAGCCTAAGTTGTCACCAGCAGGACTGATGTGCACACAGAAGGAATGAAGTATGGATGTGAAAGAACGCAGGCCTTACTGCTCCCTGACCAAGAGCAGACGAGAGAAGGAACGGCGCTACACAAATTCCTCCGCAGACAATGAGGAGTGCCGGGTACCCACACAGAAGTCCTACAGTTCCAGCGAGACATTGAAAGCTTTTGATCATGATTCCTCGCGGCTGCTTTACGGCAACAGAGTGAAGGATTTGGTTCACAGAGAAGCAGACGAGTTCACTAGACAAAGCAGGATGCACTATGGAAACCGAGTCACAGACCTCATCCACCGGGAGTCAGATGAGTTTCCTAGACAAGGTATCCTTCACCAGGGCTACTCCCTTAGCACAGGGTCTGACGCCGACTCCGACACCGAGGGAGGGATGTCTCCAGAACACGCCATCAGACTGTGGGGCAGAGGGATAAAATCCAGGCGCAGTTCCGGCCTGTCCAGTCGTGAAAACTCGGCCCTTACCCTGACTGACTCTGACAACGAAAACAAATCAGATGATGAGAACGGTCGTCCCATTCCACGTACATCCTCGCGTAGTCTCCTCCCATTTGTTCAGCTGCCTAGCTCCCATAATCCTCCACCAGTTAGCTGCCAGATGCCATTGCTAGACAGCAACACCTCCCATCAAATCATGGACACCAACCCTGATGAGGAATTCTCCCCCAATTCATACCTGCTCAGAGCATGCTCAGGGCCCCAGCAAGCCTCCAGCAGTGGTCCTCCGAACCACCACAGCCAGTCGACTCTGAGGCCCCCTCTCCCACCCCCTCACAACCACACGCTGTCCCATCACCACTCGTCCGCCAACTCCCTCAACAGGAACTCACTGACCAATCGGCGGAGTCAGATCCACGCCCCGGCCCCAGCGCCCAATGACCTGGCCACCACACCAGAGTCCGTTCAGCTTCAGGACAGCTGGGTGCTAAACAGCAACGTGCCACTGGAGACCCGGCACTTCCTCTTCAAGACCTCCTCGGGGAGCACACCCTTGTTCAGCAGCTCTTCCCCGGGATACCCTTTGACCTCAGGAACGGTTTACACGCCCCCGCCCCGCCTGCTGCCCAGGAATACTTTCTCCAGGAAGGCTTTCAAGCTGAAGAAGCCCTCCAAATACTGCAGCTGGAAATGTGCTGCCCTCTCCGCCATTGCCGCGGCCCTCCTCTTGGCTATTTTGCTGGCGTATTTCGCAGCAATGCATCTGCTCGGACTCAATTGGCAACTCCAGCCTGCAGATGGGCACACCTTTAACAATGGGATAAGGACCGGCTTACCAGGAAACGATGATGTGGCAACAATGCCATCTGGAGGCAAAGTGCCCTGGTCGTTGAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGACTCATTGACTGCATGGATCCCGATTGCTGCCTACAGAGTTCCTGCCAGAATCAGCCCTATTGTCGGGGACTGCCGGATCCTCAGGACATCATTAGCCAAAGCCTTCAATCGCCTTCTCAGCAAGCTGCCAAATCCTTTTATGATCGAATCAGTTTCCTTATAGGATCTGATAGCACCCATGTTATACCTGGAGAAAGTCCTTTCAATAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTACTCCCTCTCCAGGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGTTGTGACCTCAGTGGTTTTTGTCGGCTTGATCCCATCATCATCTCCTCCCCTCTGTCCACTTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTACTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTACACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGGTATGTTTTGGTTTCAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGTATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGGAGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAAGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATAGCTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTPAACTCCATTGAGTGGCGCCTAAGAPAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCGAGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAACTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGCCGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATCACAGGTGCCCACCAGACAACAGAGAGACATAACCAGGCTTTCATGGCTCTGGAAGGACAGGTCATTACTAkAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCATTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGTAACAAAATAATCTGCTGCCATTCCTTGTCTGAATGGCTCAGCAGGAGTAACTGTTATCTCCTCTCCTAAGGAGATGAAGACCTAACAGGGGCACTGCGG NOV7ab, CG50426-24SEQ ID NO: 1682759 aaMW at 306902.2kDProtein SequenceMDVKERRPYCSLTKSRREKERRYTNSSADNEECRVPTQKSYSSSETLKAFDHDSSRLLYGNRVKDLVHREADEFTRQSRMHYGNRVTDLIHRESDEFPRQGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPRTSSRSLLPFVQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNPRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFAAMHLLGLNWQLQPADGHTFNNGIRTGLPGNDDVATMPSGGKVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLIDCMDPDCCLQSSCQNQPYCRGLPDPQDIISQSLQSPSQQAAKSFYDRISFLIGSDSTHVIPGESPFNSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTYSLSRFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFCRLDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVGMFWFQRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDIAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDATLNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYSYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLEVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVPAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGAHQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7ac, CG50426-25SEQ ID NO: 1696999 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATGTGACCAGCATCTTGGAGTTACGAAATAAAGAGTTTAAACATAGCAACAACCCAGCACACAAGTACTACTTGGCAGTGGACCCCGTGTCCGGCTCGCTCTACGTGTCCGACACCAACAGCAGGAGAATCTACCGCGTCAAGTCTCTGAGTGGAACCAAAGACCTGGCTGGGAATTCGGAAGTTGTGGCAGGGACGGGAGAGCAGTGTCTACCCTTTGATGAAGCCCGCTGCGGGGATGGAGGGAAGGCCATAGATGCAACCCTGATGAGCCCGAGAGGTATTGCAGTAGACAAGAATGGGCTCATGTACTTTGTCGATGCCACCATGATCCGGAAGGTTGACCAGAATGGAATCATCTCCACCCTGCTGGGCTCCAATGACCTCACTGCCGTCCGGCCGCTGAGCTGTGATTCCAGCATGGATGTAGCCCAGGTTCGTCTGGAGTGGCCAACAGACCTTGCTGTCAATCCCATGGATAACTCCTTGTATGTTCTAGAGAACAATGTCATCCTTCGAATCACCGAGAACCACCAAGTCAGCATCATTGCGGGACGCCCCATGCACTGCCAAGTTCCTGGCATTGACTACTCACTCAGCAAACTAGCCATTCACTCTGCCCTGGAGTCAGCCAGTGCCATTGCCATTTCTCACACTGGGGTCCTCTACATCACTGAGACAGATGAGAAGAAGATTAACCGTCTACGCCAGGTAACAACCAACGGGGAGATCTGCCTTTTAGCTGGGGCAGCCTCGGACTGCGACTGCAAAAACGATGTCAATTGCAACTGCTATTCAGGAGATGATGCCTACGCGACTGATGCCATCTTGAATTCCCCATCATCCTTAGCTGTAGCTCCAGATGGTACCATTTACATTGCAGACCTTGGAAATATTCGGATCAGGGCGGTCAGCAAGAACAAGCCTGTTCTTAATGCCTTCAACCAGTATGAGGCTGCATCCCCCGGAGAGCAGGAGTTATATGTTTTCAACGCTGATGGCATCCACCAATACACTGTGAGCCTGGTGACAGGGGAGTACTTGTACAATTTCACATATAGTACTGACAATGATGTCACTGAATTGATTGACAATAATGGGAATTCCCTGAAGATCCGTCGGGACAGCAGTGGCATGCCCCGTCACCTGCTCATGCCTGACAACCAGATCATCACCCTCACCGTGGGCACCAATGGAGGCCTCAAAGTCGTGTCCACACAGAACCTGGAGCTTGGTCTCATGACCTATGATGGCAACACTGGGCTCCTGGCCACCAAGAGCGATGAAACAGGATGGACGACTTTCTATGACTATGACCACGAAGGCCGCCTGACCAACGTGACGCGCCCCACGGGGGTGGTAACCAGTCTGCACCGGGAAATGGAGAAATCTATTACCATTGACATTGAGAACTCCAACCGTGATGATGACGTCACTGTCATCACCAACCTCTCTTCAGTAGAGGCCTCCTACACAGTGGTACAAGATCAAGTTCGGAACAGCTACCAGCTCTGTAATAATGGTACCCTGAGGGTGATGTATGCTAATGGGATGGGTATCAGCTTCCACAGCGAGCCCCATGTCCTAGCGGGCACCATCACCCCCACCATTGGACGCTGCAACATCTCCCTGCCTATGGAGAATGGCTTAAACTCCATTGAGTGGCGCCTAAGAAAGGAACAGATTAAAGGCAAAGTCACCATCTTTGGCAGGAAGCTCCGGGTCCATGGAAGAAATCTCTTGTCCATTGACTATGATCGAAATATTCGGACTGAAAAGATCTATGATGACCACCGGAAGTTCACCCTGAGGATCATTTATGACCAGGTGGGCCGCCCCTTCCTCTGGCTGCCCAGCAGCGGGCTGGCAGCTGTCAACGTGTCATACTTCTTCAATGGGCGCCTGGCTGGGCTTCAGCGTGGGGCCATGAGCGAGAGGACAGACATCGACAAGCAAGGCCGCATCGTGTCCCGCATGTTCGCTGACGGGAAAGTGTGGAGCTACTCCTACCTTGACAAGTCCATGGTCCTCCTGCTTCAGAGCCAACGTCAGTATATATTTGAGTATGACTCCTCTGACCGCCTCCTTGCCGTCACCATGCCCAGCGTGGCCCGGCACAGCATGTCCACACACACCTCCATCGGCTACATCCGTAATATTTACAACCCGCCTGAAAGCAATGCTTCGGTCATCTTTGACTACAGTGATGACGGCCGCATCCTGAAGACCTCCTTTTTGGGCACCGGACGCCAGGTGTTCTACAAGTATGGGAAACTCTCCAAGTTATCAGAGATTGTCTACGACAGTACCGCCGTCACCTTCGGGTATGACGAGACCACTGGTGTCTTGAAGATGGTCAACCTCCAAAGTGGGGGCTTCTCCTGCACCATCAGGTACCGGAAGATTGGCCCCCTGGTGGACAAGCAGATCTACAGGTTCTCCGAGGAAGGCATGGTCAATGCCAGGTTTGACTACACCTATCATGACAACAGCTTCCGCATCGCAAGCATCAAGCCCGTCATAAGTGAGACTCCCCTCCCCGTTGACCTCTACCGCTATGATGAGATTTCTGGCAAGGTGGAACACTTTGGTAAGTTTGGAGTCATCTATTATGACATCAACCAGATCATCACCACTGCCGTGATGACCCTCAGCAAACACTTCGACACCCATGGGCGGATCAAGGAGGTCCAGTATGAGATGTTCCGGTCCCTCATGTACTGGATGACGGTGCAATATGACAGCATGGGCAGGGTGATCAAGAGGGAGCTAAAACTGGGGCCCTATGCCAATACCACGAAGTACACCTATGACTACGATGGGGACGGGCAGCTCCAGAGCGTGGCCGTCAATGACCGCCCGACCTGGCGCTACAGCTATGACCTTAATGGGAATCTCCACTTACTGAACCCAGGCAACAGTGTGCGCCTCATGCCCTTGCGCTATGACCTCCGGGATCGGATAACCAGACTCGGGGATGTGCAGTACAAAATTGACGACGATGGCTATCTGTGCCAGAGAGGGTCTGACATCTTCGAATACAATTCCAAGGGCCTCCTAACAAGAGCCTACAACAAGGCCAGCGGGTGGAGTGTCCAGTACCGCTATGATGGCGTAGGACGGCGGGCTTCCTACAAGACCAACCTGGGCCACCACCTGCAGTACTTCTACTCTGACCTCCACAACCCGACGCGCATCACCCATGTCTACAATCACTCCAACTCGGAGATTACCTCACTGTACTACGACCTCCAGGGCCACCTCTTTGCCATGGAGAGCAGCAGTGGGGAGGAGTACTATGTTGCCTCTGATAACACAGGGACTCCTCTGGCTGTGTTCAGCATCAACGGCCTCATGATCAAACAGCTGCAGTACACGGCCTATGGGGAGATTTATTATGACTCCAACCCCGACTTCCAGATGGTCATTGGCTTCCATGGGGGACTCTATGACCCCCTGACCAAGCTGGTCCACTTCACTCAGCGTGATTATGATGTGCTGGCAGGACGATGGACCTCCCCAGACTATACCATGTGGAAAAACGTGGGCAAGGAGCCGGCCCCCTTTAACCTGTATATGTTCAAGAGCAACAATCCTCTCAGCAGTGAGCTAGATTTGAAGAACTACGTGACAGATGTGAAAAGCTGGCTTGTGATGTTTGGATTTCAGCTTAGCAACATCATTCCTGGCTTCCCGAGAGCCAAAATGTATTTCGTGCCTCCTCCCTATGAATTGTCAGAGAGTCAAGCAAGTGAGAATGGACAGCTCATTACAGGTGTCCAACAGACAACAGAGAGACATAACCAGGCCTTCATGGCTCTGGAAGGACAGGTCATTACTAAAAAGCTCCACGCCAGCATCCGAGAGAAAGCAGGTCACTGGTTTGCCACCACCACGCCCATCATTGGCAAAGGCATCATGTTTGCCATCAAAGAAGGGCGGGTGACCACGGGCGTGTCCAGCATCGCCAGCGAAGATAGCCGCAAGGTGGCATCTGTGCTGAACAACGCCTACTACCTGGACAAGATGCACTACAGCATCGAGGGCAAGGACACCCACTACTTTGTGAAGATTGGCTCAGCCGATGGCGACCTGGTCACACTAGGCACCACCATCGGCCGCAAGGTGCTAGAGAGCGGGGTGAACGTGACCGTGTCCCAGCCCACGCTGCTGGTCAACGGCAGGACTCGAAGGTTCACGAACATTGAGTTCCAGTACTCCACGCTGCTGCTCAGCATCCGCTATGGCCTCACCCCCGACACCCTGGACGAAGAGAAGGCCCGCGTCCTGGACCAGGCGAGACAGAGGGCCCTGGGCACGGCCTGGGCCAAGGAGCAGCAGAAAGCCAGGGACGGGAGAGAGGGGAGCCGCCTGTGGACTGAGGGCGAGAAGCAGCAGCTTCTGAGCACCGGGCGCGTGCAAGGGTACGAGGGATATTACGTGCTTCCCGTGGAGCAATACCCAGAGCTTGCAGACAGTAGCAGCAACATCCAGTTTTTAAGACAGAATGAGATGGGAAAGAGGNOV7ac, CG50426-25SEQ ID NO: 1702333 aaMW at 259545.2kDProtein SequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRPASYKTNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7ad, SNP13375665 ofSEQ ID NO: 1712478 bp1CG50426-15, DNA SequenceORF Start: at 1ORF Stop: end of sequenceSNP Pos: 799SNP Change: G to AAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAAACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATNOV7ad, SNP13375665 ofSEQ ID NO: 172826 aaMW at 89832.6kDCG50426-15, ProteinSNP Pos: 267SNP Change: Asp to AsnSequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPNQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNNOV7ae, SNP13375213 ofSEQ ID NO: 1732478 bpCG50426-15, DNA SequenceORF Start: at 1ORF Stop: end of sequenceSNP Pos: 1751SNP Change: T to CAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGCCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAAGACAGATGCGTATGGCCAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATGGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATNOV7ae, SNP13375213 ofSEQ ID NO: 174826 aaMW at 89805.5kDCG50426-15, ProteinSNP Pos: 584SNP Change: Val to AlaSequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFARPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNNOV7af, SNP13374985 ofSEQ ID NO: 1752478 bpCG50426-15, DNA SequenceORF Start: at 1ORF Stop: end of sequenceSNP Pos: 2322SNP Change: G to AAAAAACAGCAGCATAGACAGTGGTGAAGCAGAAGTTGGTCGGCGGGTAACACAAGAAGTCCCACCAGGGGTGTTTTGGAGGTCACAAATTCACATCAGTCAGCCCCAGTTCTTAAAGTTCAACATCTCCCTCGGGAAGGACGCTCTCTTTGGTGTTTACATAAGAAGAGGACTTCCACCATCTCATGCCCAGTATGACTTCATGGAACGTCTGGACGGGAAGGAGAAGTGGAGTGTGGTTGAGTCTCCCAGGGAACGCCGGAGCATACAGACCTTGGTTCAGAATGAAGCCGTGTTTGTGCAGTACCTGGATGTGGGCCTGTGGCATCTGGCCTTCTACAATGATGGAAAAGACAAAGAGATGGTTTCCTTCAATACTGTTGTCCTAGATTCAGTGCAGGACTGTCCACGTAACTGCCATGGGAATGGTGAATGTGTGTCCGGGGTGTGTCACTGTTTCCCAGGATTTCTAGGAGCAGACTGTGCTAAAGCTGCCTGCCCTGTCCTGTGCAGTGGGAATGGACAATATTCTAAAGGGACGTGCCAGTGCTACAGCGGCTGGAAAGGTGCAGAGTGCGACGTGCCCATGAATCAGTGCATCGATCCTTCCTGCGGGGGCCACGGCTCCTGCATTGATGGGAACTGTGTCTGCTCTGCTGGCTACAAAGGCGAGCACTGTGAGGAAGTTGATTGCTTGGATCCCACCTGCTCCAGCCACGGAGTCTGTGTGAATGGAGAATGCCTGTGCAGCCCTGGCTGGGGTGGTCTGAACTGTGAGCTGGCGAGGGTCCAGTGCCCAGACCAGTGCAGTGGGCATGGCACGTACCTGCCTGACACGGGCCTCTGCAGCTGCGATCCCAACTGGATGGGTCCCGACTGCTCTGTTGAAGTGTGCTCAGTAGACTGTGGCACTCACGGCGTCTGCATCGGGGGAGCCTGCCGCTGTGAAGAGGGCTGGACAGGCGCAGCGTGTGACCAGCGCGTGTGCCACCCCCGCTGCATTGAGCACGGGACCTGTAAAGATGGCAAATGTGAATGCCGAGAGGGCTGGAATGGTGAACACTGCACCATTGGTAGGCAAACGGCAGGCACCGAAACAGATGGCTGCCCTGACTTGTGCAACGGTAACGGGAGATGCACACTGGGTCAGAACAGCTGGCAGTGTGTCTGCCAGACCGGCTGGAGAGGGCCCGGATGCAACGTTGCCATGGAAACTTCCTGTGCTGATAACAAGGATAATGAGGGAGATGGCCTGGTGGATTGTTTGGACCCTGACTGCTGCCTGCAGTCAGCCTGTCAGAACAGCCTGCTCTGCCGGGGGTCCCGGGACCCACTGGACATCATTCAGCAGGGCCAGACGGATTGGCCCGCAGTGAAGTCCTTCTATGACCGTATCAAGCTCTTGGCAGGCAAGGATAGCACCCACATCATTCCTGGAGAGAACCCTTTCAACAGCAGCTTGGTTTCTCTCATCCGAGGCCAAGTAGTAACTACAGATGGAACTCCCCTGGTCGGTGTGAACGTGTCTTTTGTCAAGTACCCAAAATACGGCTACACCATCACCCGCCAGGATGGCACGTTCGACCTGATCGCAAATGGAGGTGCTTCCTTGACTCTACACTTTGAGCGAGCCCCGTTCATGAGCCAGGAGCGCACTGTGTGGCTGCCGTGGAACAGCTTTTACGCCATGGACACCCTGGTGATGAAGACCGAGGAGAACTCCATCCCCAGCTGTGACCTCAGTGGCTTTGTCCGGCCTGATCCAATCATCATCTCCTCCCCACTGTCCACCTTCTTTAGTGCTGCCCCTGGGCAGAATCCCATCGTGCCTGAGACCCAGGTTCTTCATGAAGAAATCGAGCTCCCTGGTTCCAATGTGAAACTTCGCTATCTGAGCTCTAGAACTGCAGGGTACAAGTCACTGCTGAAGATCACCATGACCCAGTCCACAGTGCCCCTGAACCTCATTAGGGTTCACCTGATGGTGGCTGTCGAGGGGCATCTCTTCCAGAAGTCATTCCAGGCTTCTCCCAACCTGGCCTCCACCTTCATCTGGGACAGACAGATGCGTATGGCCAAAAGGGTGTATGGACTCTCAGATGCTGTTGTGTCTGTCGGGTTTGAATATGAGACCTGTCCCAGTCTAATTCTCTGGGAGAAAAGGACAGCCCTCCTTCAGGGATTCGAGCTGGACCCCTCCAACCTCGGTGGCTGGTCCCTAGACAAACACCACATCCTCAATGTTAAAAGTGGAATCCTACACAAAGGCACTGGGGAAAACCAGTTCCTGACCCAGCAGCCTGCCATCATCACCAGCATCATAGGCAATGGTCGCCGCCGGAGCATTTCCTGTCCCAGCTGCAACGGCCTTGCTGAAGGCAACAAGCTGCTGGCCCCAGTGGCTCTGGCTGTTGGAATCGATGGGAGCCTCTATGTGGGTGACTTCAATTACATCCGACGCATCTTTCCCTCTCGAAATNOV7af, SNP13374985 ofSEQ ID NO: 176826 aaMW at 89815.5kDCG50426-15, ProteinSNP Pos: 774SNP Change: Met to IleSequenceKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIIGNGRRRSISCPSCNGLAEGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRN


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 7B.

TABLE 7BComparison of the NOV7 protein sequences.NOV7a------------------------------------------------------------NOV7b------------------------------------------------------------NOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7l------------------------------------------------------------NOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7o------------------------------------------------------------NOV7p------------------------------------------------------------NOV7qMDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRNHYGNRNOV7r------------------------------------------------------------NOV7s------------------------------------------------------------NOV7t------------------------------------------------------------NOV7u------------------------------------------------------------NOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7ab------------------------------------------------------------NOV7ac------------------------------------------------------------NOV7a------------------------------------------------------------NOV7b------------------------------------------------------------NOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7l------------------------------------------------------------NOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7O------------------------------------------------------------NOV7p------------------------------------------------------------NOV7qVTDLIHRESDEFPRQGTNFTTAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTENOV7r------------------------------------------------------------NOV7s------------------------------------------------------------NOV7t------------------------------------------------------------NOV7u------------------------------------------------------------NOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7ab------------------------------------------------------------NOV7ac------------------------------------------------------------NOV7a------------------------------------------------------------NOV7b--------------------------MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7l------------------------------------------------------------NOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7o------------------------------------------------------------NOV7p------------------------------------------------------------NOV7qGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLNOV7r------------------------------------------------------------NOV7s------------------------------------------------------------NOV7t------------------------------------------------------------NOV7u------------------------------------------------------------NOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7ab--------------------------MDVKERRPYCSLTKSRREKERRYTNSSADNEECRNOV7ac------------------------------------------------------------NOV7a------------------------------------------------------------NOV7bPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7l-------MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7o------------------------------------------------------------NOV7p------------------------------------------------------------NOV7qPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNOV7r-------MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSNOV7s-------MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSNOV7t------------------------------------------------------------NOV7u-------MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abVPTQKSYSSSETLKAFDHDSSRLLYGNRVKDLVHREADEFTRQSRMHYGNRVTDLIHRESNOV7ac------------------------------------------------------------NOV7a------------------------------------------------------------NOV7bGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7o----MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHNOV7p------------------------------------------------------------NOV7qNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQNOV7rRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSNOV7sRNHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSNOV7t------------------------------------------------------------NOV7uRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abDEFPRQGTLHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLNOV7ac------------------------------------------------------------NOV7a------------------MDVKDRRHRSLTRGRCGKECRYTSSSLDSEDCRVPTQKSYSSNOV7bTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lDADSDTEGGNSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADNOV7p------------------------------------------------------------NOV7qLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKNOV7rDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPNOV7sDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPNOV7t------------------------------------------------------------NOV7uDADSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abTDSDNENKSDDENGRPIPRTSSRSLLPFVQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPNOV7ac------------------------------------------------------------NOV7aSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQGTNFTLAELGICEPSPHRSGYCSDMGINOV7bDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNPNSNOV7c----------------------------------------KNSSIDSGEAEVGRRVTQEVNOV7d-------------------------------------TKLKNSSIDSGEAEVGRRVTQEVNOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oSDTEGGMSPEHAIRLWGRGIKSRRSSGLSSRBNSALTLTDSDNENKSDDENGRPIPPTSSNOV7p------------------------------------------------------------NOV7qAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVNOV7rTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQNOV7sTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLPACSGPQQNOV7t------------------------------------------------------------NOV7uTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abDEEFSPNSYLLEACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNPNSNOV7ac------------------------------------------------------------NOV7aLHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGIKSPRSSGLSSRENSALTLTDSDNENKNOV7bLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSNOV7cPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVNOV7dPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVNOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------TKLKNSSIDSGENOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7l------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLANOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSNOV7p------------------------------------------------------------NOV7qPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVNOV7rASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLANOV7sASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLANOV7t------------------------------------------------------------NOV7uASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLANOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSNOV7ac------------------------------------------------------------NOV7aSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSCQMPLLDSNTSHQIMDTNPDEEFSPNSNOV7bPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFNOV7cESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHNOV7dESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHNOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7gAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMENOV7h------------------------------------------------------------NOV7i---------------------------------------------MDVKDRRHRSLTRGRNOV7j---------------------------------------------MDVKDRRHRSLTRGRNOV7k---------------------------------------------MDVKDRRHRSLTRGRNOV7lTTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPNOV7m-----------------------------------KHQHSAISGIMDVKDRRHRSLTRGRNOV7n-----------------------------------KHQHSAISGIMDVKDRRHRSLTRGRNOV7oSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPNOV7p-----------------------------------KHQHSAISGIMDVKDRRHRSLTRGRNOV7qESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHNOV7rTTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPNOV7sTTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPNOV7t-----------------------------------KHQHSAISGIMDVKDRRHRSLTRGRNOV7uTTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFNOV7ac------------------------------------------------------------NOV7aYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPHNHTLSHHHSSANSLNRNSLTNRRSQINOV7bIAMHLLGLNWQLQPADGHTFNNGIRTGLPGNDDVATMPSGGKVPWSLKNSSIDSGEAEVGNOV7cGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCINOV7dGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCINOV7e--------------------------------------------TKLKNSSIDSGEAEVGNOV7f------------------------------------------------------------NOV7gRLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLNOV7h------------------------------------------------------------NOV7iCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7jCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7kCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7lRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGNOV7mCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7nCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7oESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTNOV7pCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7qGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNQCINOV7rRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGNOV7sRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGNOV7tCGKECRYTSSSLDSEDCRVPTQKSYSSSETLKAYDHDSRMHYGNRVTDLIHRESDEFPRQNOV7uRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGNOV7v-----------------------------------------------KNSSIDSGEAEVGNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abAAMHLLGLNWQLQPADGHTFNNGIRTGLPGNDDVATMPSGGKVPWSLKNSSIDSGEAEVGNOV7ac-----------------------------------------------KNSSIDSGEAEVGNOV7aHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETRHFLFKTSSGSTPLFSSSSPGYPLTSGNOV7bRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7cDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELNOV7dDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELNOV7eRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7f-----------------------------------------TKLKNSSIDSGEAEVGRRVNOV7gDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGNOV7h------------------------------------------------------------NOV7iGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7jGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7kGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7lRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7mGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7nGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7oFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVNOV7pGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7qDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNCELNOV7rRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7sRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7tGTNFTLAELGICEPSPHRSGYCSDMGILHQGYSLSTGSDADSDTEGGMSPEHAIRLWGRGNOV7uRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7vRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa--------------------------------------------KNSSIDSGEAEVGRRVNOV7abRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7acRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGNOV7aTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCAALSAIAAALLLAILLAYFIVPWSLKNNOV7bKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7cARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTNOV7dARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTNOV7eKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7fTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKNOV7gAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCNOV7h-----------------MLHAANKGRKPSAEAGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7iIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7jIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7kIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7lKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7mIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7nIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7oTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKNOV7pIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7qARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCEEGWTNOV7rKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7sKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7tIKSRRSSGLSSRENSALTLTDSDNENKSDDENGRPIPPTSSPSLLPSAQLPSSHNPPPVSNOV7uKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7vKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z-----------------------------------------------------------NNOV7aaTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKNOV7abKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7acKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQNOV7aSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHNOV7bDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7cGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTNOV7dGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTNOV7eDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7fWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPNOV7gSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVDGCPDLCNGNGRCTNOV7hCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7iCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7jCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7kCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7lDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7mCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7nCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7oWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPNOV7pCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7qGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNGRCTNOV7rDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7sDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7tCQMPLLDSNTSHQIMDTNPDEEFSPNSYLLRACSGPQQASSSGPPNHHSQSTLRPPLPPPNOV7uDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7vDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7zSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPQFLKFNISLGKDALFGVYIRRGLPPSHNOV7aaWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPNOV7abDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7acDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDNOV7aAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVNOV7bVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7cLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGNOV7dLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGNOV7eVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7fRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNOV7gLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGNOV7hHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7iHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7jHNHTLSHHHSSANSLNENSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7kHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7lVPNNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7mHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7nHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7oRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNOV7pHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7qLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGNOV7rVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7sVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7tHNHTLSHHHSSANSLNRNSLTNRRSQIHAPAPAPNDLATTPESVQLQDSWVLNSNVPLETNOV7uVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7vVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7zAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNEAVFVQYLDVGLWHLAFYNDGKDKEMVNOV7aaRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCQCYSGWKGAECDVPMNOV7abVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7acVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWNOV7aSFNTVVLDS-VQDCPRNCHGNGECVSGVCHCFPGFLGADCAKAACPVLCSGNGQYSKGTCNOV7bGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7cSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGNOV7dSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGNQV7eGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7fNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNOV7gSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGNOV7hRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7iRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7jRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7kRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7lGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7mRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7nRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7oNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNOV7pRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7qSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGNOV7rGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7sGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7tRHFLFKTSSGSTPLFSSSSPGYPLTSGTVYTPPPRLLPRNTFSRKAFKLKKPSKYCSWKCNOV7uGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7vGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7w------------------------DTGLCSCDPNWMGPDCSVE-----------------NOV7x----------------------DRIKLLAGKDSTHIIPGE--------------------NOV7y---------------------SAGYKGEHCEEVDCLDPTCS-------------------NOV7zSFNTVVLDDSVQDCPRNCHGNGECVSGVCHCFPGFLGADCAKDLPALTFCKTIINKAAL-NOV7aaNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSHGVCVNGECLCSPGWGGLNOV7abGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVVCSVDCGTHGVCIGGACRNOV7acGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACNOV7aQCYSGWKGAECDVPMNQCIDPSCGGHGSCIDGNCVCSAGYKGEHCEEVDCLDPTCSSNGVNOV7bRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCN--------NOV7cTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFNOV7dTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFNOV7eRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGNOV7fNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCENOV7gTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFNOV7hAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPNOV7iAALSAIAAALLLAIL---------------------------------------------NOV7jAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPNOV7kAALSAIAAALLLAIL---------------------------------------------NOV7lRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNOV7mAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPNOV7nAALSAIAAALLLAIL---------------------------------------------NOV7oNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCENOV7pAALSAIAAALLLAILLAYFIVPWSLKNSSIDSGEAEVGRRVTQEVPPGVFWRSQIHISQPNOV7qTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFNOV7rRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCN--------NOV7sRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNOV7tAALSAIAAALLLAIL---------------------------------------------NOV7uRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNOV7vRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVEVCSVDCGTHGVCIGGACRCENOV7abCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNG--------NOV7acRCEEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNOV7aCVNGECLCSPGWGGLNCELARVQCPDQCSGHGTYLPDTGLCSCDPNWMGPDCSVDGCPDLNOV7b-GNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7cYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIENOV7dYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIENOV7eQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRNOV7fEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIDGCPDLCNGNGRCTLGQNSNOV7gYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIENOV7hQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNNOV7i------------------------------------------------------------NOV7jQFLKFNTSLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNNOV7k------------------------------------------------------------NOV7lNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7mQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNNOV7n------------------------------------------------------------NOV7oEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNNOV7pQFLKFNISLGKDALFGVYIRRGLPPSHAQYDFMERLDGKEKWSVVESPRERRSIQTLVQNNOV7qYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIENOV7r-GNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7sNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7t------------------------------------------------------------NOV7uNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7vNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaEGWTGAACDQRVCHPRCIEHGTCKDGKCECREGWNGEHCTIGRQTAGTETDGCPDLCNGNNOV7ab------NGR-CTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLIDCMDPDCCLNOV7acNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNOV7aCNGNGRCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACNOV7bN-----------------------------------------------------------NOV7cL-----------------------------------------------------------NOV7dL-----------------------------------------------------------NOV7eD-----------------------------------------------------------NOV7fW-----------------------------------------------------------NOV7gL-----------------------------------------------------------NOV7hEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDGTI----------------------NOV7i------------------------------------------------------------NOV7jEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDS------------------------NOV7k------------------------------------------------------------NOV7lN-----------------------------------------------------------NOV7mEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGNOV7n------------------------------------------------------------NOV7oG-----------------------------------------------------------NOV7pEAVFVQYLDVGLWHLAFYNDGKDKEMVSFNTVVLDSVQDCPRNCHGNGECVSGVCHCFPGNOV7qL-----------------------------------------------------------NOV7rN-----------------------------------------------------------NOV7sN-----------------------------------------------------------NOV7t------------------------------------------------------------NOV7uN-----------------------------------------------------------NOV7vN-----------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaG-----------------------------------------------------------NOV7abQ-----------------------------------------------------------NOV7acN-----------------------------------------------------------NOV7aQNSLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7b--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7c--PGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLNOV7d--PGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLNOV7e--PLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTNOV7f--QCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSLLCRGSRDPNOV7g--PGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLNOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7l--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7mFLGADCAKGMCRHFP---------------------------------------------NOV7n------------------------------------------------------------NOV7o--RCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSNOV7pFLGADCAKAACPVLC---------------------------------------------NOV7q--PGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLNOV7r--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7s--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7t------------------------------------------------------------NOV7u--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7v--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa--RCTLGQNSWQCVCQTGWRGPGCNVAMETSCADNKDNEGDGLVDCLDPDCCLQSACQNSNOV7ab--SSCQNQPyCRGLPDPQDIISQSLQSPSQQAAKSFYDRISFLIGSDSTHVIPGESPFNSNOV7ac--SLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRNOV7aGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7bGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7cASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSNOV7dAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSNOV7ePLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLPWNSFYNOV7fLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVVTTDGTPLVNOV7gAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSNOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVNOV7p------------------------------------------------------------NOV7qASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSNOV7rGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7sGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7t------------------------------------------------------------NOV7uGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7vGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaLLCRGSRDPLDIIQQGQTDWPAVKSFYDRIKLLAGKDSTHIIPGENPFNSSLVSLIRGQVNOV7abLVSLIRGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTYSLSRFDLIANGGASLTLHFNOV7acGQVVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTNOV7aVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7bVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7cLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPNOV7dLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRPRSISCPSCNGLAEGNKLLAPNOV7eAMDTLVMXTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLHEEIELNOV7fGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFEPAPFMSQERTVWLPWNSFYAMDNOV7gLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPNOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFERAPFMSQERTVWLNOV7p------------------------------------------------------------NOV7qLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPNOV7rVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7sVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7t------------------------------------------------------------NOV7uVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7vVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaVTTDGTPLVGVNVSFVKYPKYGYTITRQDGTFDLIANGGASLTLHFEPAPFMSQERTVWLNOV7abEPAPFMSQERTVWLPWNSFYANDTLVMKTEENSIPSCDLSGFCRLDPIIISSPLSTFFSANOV7acVWLPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETNOV7aQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7bQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7cVALAVGIDGSLYVGDFNYIRRIFPSRN---------------------------------NOV7dVALAVGIDGSLYVGDFNYIRRIFPSRNLEG------------------------------NOV7ePGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYNOV7fTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVFETQVLHEEIELPGSNOV7gVALAVGIDGSLYVGDFNYIRRIFPSRNLEG------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSFLSTFFSAAPGQNPIVPETQVLNOV7p------------------------------------------------------------NOV7qVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVSGSLNOV7rQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7sQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7t------------------------------------------------------------NOV7uQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7vQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaPWNSFYAMDTLVMKTEENSIPSCDLSGFVRPDPIIISSPLSTFFSAAPGQNPIVPETQVLNOV7abAPGQNPIVPETQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMNOV7acQVLHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSNOV7aFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7bFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7eTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDNOV7fNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQASPNLAYTFINOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLIRVHLMVAVEGHLFQKSFQANOV7p------------------------------------------------------------NOV7qYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMSPRGNOV7rFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7sFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7t------------------------------------------------------------NOV7uFQASPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7vFQASPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaHEEIELPGSNVKLRYLSSRTAGYKSLLKITMTQSTVPLNLTRVHLMVAVEGHLFQKSFQANOV7abVAVEGHLFQKSFQASPNLAYTFIWDKTDAYGQRVYGLSDAVGMFWFQRTALLQGFELDPSNOV7acFQASPNASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPNOV7aSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAITTSIMGNGRRRSISCPSCNGLAENOV7bSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAENOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7eKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVANOV7fWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLGGWSLDKHHNOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAENOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oSPNLASTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLNOV7p------------------------------------------------------------NOV7qIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWPTDLNOV7rSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAENOV7sSNLGGWSLDKHHILNVKSGTLHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAENOV7t------------------------------------------------------------NOV7uSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAENOV7vSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRPRSISCPSCNGLAENOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaSPNLAYTFIWDKTDAYGQRVYGLSDAVVSVGFEYETCPSLILWEKRTALLQGFELDPSNLNOV7abNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNOV7acSNLGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAENOV7aGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVNOV7bGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7eLAVGIDGSLYVGDFNYIRRIFPSRNLEG--------------------------------NOV7fILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKLLAPVALAVNOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKNOV7p------------------------------------------------------------NOV7qAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESASAINOV7rGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSR1VTSILELRNKEFKHSNNPAHKYYLAVNOV7sGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVNOV7t------------------------------------------------------------NOV7uGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVNOV7vGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRN--------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaGGWSLDKHHILNVKSGILHKGTGENQFLTQQPAIITSIMGNGRRRSISCPSCNGLAEGNKNOV7abNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRRNKEFKHSNNPAHKYYLAVNOV7acGNKLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVNOV7aDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7bDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7fGIDGSLYVGDFNYIRRIFPSRNLEG-----------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oLLAPVALAVGIDGSLYVGDFNYIRRIFPSRNVTSILELRNKEFKHSNNPAHKYYLAVDPVNOV7p------------------------------------------------------------NOV7qAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDAYATDNOV7rDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7sDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7t------------------------------------------------------------NOV7uDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aaLLAPVALAVGIDGSLYVGDFNYIRRIFPSRN-----------------------------NOV7abDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7acDPVSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDANOV7aTLMSPRGIAVDKNGLMYFVDATMIRXVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7bTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oSGSLYVSDTNSRRIYRVKSLSGTKDLAGNSEVVAGTGEQCLPFDEARCGDGGKAIDATLMNOV7p------------------------------------------------------------NOV7qAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELYVFNADGNOV7rTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7sTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7t------------------------------------------------------------NOV7uTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7acTLMSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRNOV7aLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKIAIHSANOV7bLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKIAIHSANOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lLEWPTDIAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSANOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oSPRGIAVDKNGLMYFVDATMIRKVDQNGIISTLLGSNDLTAVRPLSCDSSMDVAQVRLEWNOV7p------------------------------------------------------------NOV7qIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQIITLTNOV7rLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSANOV7sLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKTAIHSANOV7t------------------------------------------------------------NOV7uLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRFMHCQVPGIDYSLSKLAIHSANOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSANOV7acLEWPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSANOV7aLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7bLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oPTDLAVNPMDNSLYVLENNVILRITENHQVSIIAGRPMHCQVPGIDYSLSKLAIHSALESNOV7p------------------------------------------------------------NOV7qVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPTGVVNOV7rLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7sLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7t------------------------------------------------------------NOV7uLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7acLESASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGNOV7aDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOV7bDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOv7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oASAIAISHTGVLYITETDEKKINRLRQVTTNGEICLLAGAASDCDCKNDVNCNCYSGDDANOV7p------------------------------------------------------------NOV7qTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNGTLRVMYNOV7rDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOV7sDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIPAVSKNKPVLNAFNQYEAASPGEQELNOV7t------------------------------------------------------------NOV7uDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOV7acDDAYATDAILNSPSSLAVAPDGTIYIADLGNIRIRAVSKNKPVLNAFNQYEAASPGEQELNOV7aYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7bYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oYATDAILNSPSSLLAVAPDGTIYIADLGNIRIRAVSIKPVLNAFNQYEAASPGEQELYVFNOV7p------------------------------------------------------------NOV7qANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGRKLRNOV7rYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7sYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7t------------------------------------------------------------NOV7uYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIPRDSSGMPRHLLMPDNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7acYVFNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNOV7aNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVNOV7bNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oNADGIHQYTVSLVTGEYLYNFTYSTDNDVTELIDNNGNSLKIRRDSSGMPRHLLMPDNQINOV7p------------------------------------------------------------NOV7qVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFFNGRNOV7rNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVNOV7sNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVNOV7t------------------------------------------------------------NOV7uNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYSYDHEGRLTNVNOV7acNQIITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDNEGRLTNVNOV7aTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNNOV7bTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVPNSYQLCNNNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oITLTVGTNGGLKVVSTQNLELGLMTYDGNTGLLATKSDETGWTTFYDYDHEGRLTNVTRPNOV7p------------------------------------------------------------NOV7qLAGLQRGANSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDSSDRNOV7rTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNNOV7sTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVPNSYQLCNNNOV7t------------------------------------------------------------NOV7uTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTWQDQVRNSYQLCNNNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNNOV7acTRPTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVRNSYQLCNNNOV7aGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7bGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7l------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oTGVVTSLHREMEKSITIDIENSNRDDDVTVITNLSSVEASYTVVQDQVPNSYQLCNNGTLNOV7p------------------------------------------------------------NOV7qLLAVTMPSVAPHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRQVFYNOV7rGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7sGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7t------------------------------------------------------------NOV7uGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abGTLRVMYANGMGISFHSEPHVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7acGTLRVMYANGMGISFHSEPNVLAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTNOV7aIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7bIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7l------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oRVMYANGMGISFHSEPHVIAGTITPTIGRCNISLPMENGLNSIEWRLRKEQIKGKVTIFGNOV7p------------------------------------------------------------NOV7qKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQIYRFSNOV7rIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7sIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7t------------------------------------------------------------NOV7uIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abIFGRKLEVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7acIFGRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVNOV7aSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7bSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oRKLRVHGRNLLSIDYDRNIRTEKIYDDHRKFTLRIIYDQVGRPFLWLPSSGLAAVNVSYFNOV7p------------------------------------------------------------NOV7qEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYYDINNOV7rSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7sSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7t------------------------------------------------------------NOV7uSYFFNGRLAGLQRGANSERTDIDKQGRIVSRNFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abSYFFNGRLAGLQRGAMSERTDIDKQGRIVSRNFADGKVWSYSYLDKMVLLLQSQRQYIFENOV7acSYFFNGRLAGLQRGAMSERTDIDKQGRIVSPMFADGKVWSYSYLDKSMVLLLQSQRQYIFNOV7aEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGNOV7bEYDSSDRLLAVTMPSVARHSMSTHTSIGYIPNIYNPPESNASVIFDYSDDGRILKTSFLGNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oFNGRLAGLQRGAMSERTDIDKQGRIVSRMFADGKVWSYSYLDKSMVLLLQSQRQYIFEYDNOV7p------------------------------------------------------------NOV7qQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANTTKNOV7rEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRMIYNPPESNASVIFDYSDDGRILKTSFLGNOV7sEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGNOV7t------------------------------------------------------------NOV7uEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTNOV7acEYDSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGNOV7aTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDNOV7bTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oSSDRLLAVTMPSVARHSMSTHTSIGYIRNIYNPPESNASVIFDYSDDGRILKTSFLGTGRNOV7p------------------------------------------------------------NOV7qYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRLGDVNOV7rTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFScTIRYRKIGPLVDNOV7sTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDNOV7t------------------------------------------------------------NOV7uTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKNOV7acTGRQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDNOV7aKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGNOV7bKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oQVFYKYGKLSKLSEIVYDSTAVTFGYDETTGVLKMVNLQSGGFSCTIRYRKIGPLVDKQINOV7p------------------------------------------------------------NOV7qQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNLGHHLQNOV7rKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGNOV7sKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGNOV7t------------------------------------------------------------NOV7uKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVNOV7acKQIYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDETSGKVEHFGKFGNOV7aVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7bVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oYRFSEEGMVNARFDYTYHDNSFRIASIKPVISETPLPVDLYRYDEISGKVEHFGKFGVIYNOV7p------------------------------------------------------------NOV7qYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLAVFSNOV7rVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7sVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7t------------------------------------------------------------NOV7uVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPNOV7acVIYYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGNOV7aPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLFLRNOV7bPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLFLRNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oYDINQIITTAVMTLSKHFDTHGRIKEVQYEMFRSLMYWMTVQYDSMGRVIKRELKLGPYANOV7p------------------------------------------------------------NOV7qINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRWTSPNOV7rPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRNOV7sPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRNOV7t------------------------------------------------------------NOV7uPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLPDRNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abYANTTKYTYDYDGDGQLQSVPAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRNOV7acPYANTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRNOV7aITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGwSVQYRYDGVGRRASYKTNOV7bITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGwSVQYRYDGVGRRAsYKTNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oNTTKYTYDYDGDGQLQSVAVNDRPTWRYSYDLNGNLHLLNPGNSVRLMPLRYDLRDRITRNOV7p------------------------------------------------------------NOV7qDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGFPRANOV7rITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNOV7sITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNOV7t------------------------------------------------------------NOV7uITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNOV7acITRLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTRAYNKASGWSVQYRYDGVGRRASYKTNOV7aNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7bNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oLGDVQYKIDDDGYLCQRGSDIFEYNSKGLLTPAYNKASGWSVQYRYDGVGRRASYKTNLGNOV7p------------------------------------------------------------NOV7qKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKAGHWNOV7rNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7sNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7t------------------------------------------------------------NOV7uNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7acNLGHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGNOV7aTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7bTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oHHLQYFYSDLHNPTRITHVYNHSNSEITSLYYDLQGHLFAMESSSGEEYYVASDNTGTPLNOV7p------------------------------------------------------------NOV7qFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKDTHYNOV7rTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7sTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7t------------------------------------------------------------NOV7uTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7acTPLAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLNOV7aAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7bAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oAVFSINGLMIKQLQYTAYGEIYYDSNPDFQMVIGFHGGLYDPLTKLVHFTQRDYDVLAGRNOV7p------------------------------------------------------------NOV7qFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLLSIRNOV7rAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7sAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7t------------------------------------------------------------NOV7uAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abAGRWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7acAGRWTSPDYTNWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNINOV7aIPGFPRAXMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7bIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oWTSPDYTMWKNVGKEPAPFNLYMFKSNNPLSSELDLKNYVTDVKSWLVMFGFQLSNIIPGNOV7p------------------------------------------------------------NOV7qYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTGRVQNOV7rIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7sIPGFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7t------------------------------------------------------------NOV7uIPGFPRAXMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abIPGFPRAKMYFVPPPYELSESQASENGQLITGAHQTTERHNQAFMALEGQVITKKLHASINOV7acIPGFPRAKNYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASINOV7aREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7bREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oFPRAKMYFVPPPYELSESQASENGQLITGVQQTTERHNQAFMALEGQVITKKLHASIREKNOV7p------------------------------------------------------------NOV7qGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR--------------------------NOV7rREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7sREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7t------------------------------------------------------------NOV7uREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7acREKAGHWFATTTPIIGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSINOV7aEGKDTHYFVKIGSADGDLVTLGTTIGRYVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7bEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------Nov7lEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oAGHWFATTTPITGKGIMFAIKEGRVTTGVSSIASEDSRKVASVLNNAYYLDKMHYSIEGKNOV7p------------------------------------------------------------NOV7q------------------------------------------------------------NOV7rEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7sEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7t------------------------------------------------------------NOV7uEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7acEGKDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSNOV7aTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7bTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------Nov7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7m------------------------------------------------------------NOV7n------------------------------------------------------------NOV7oDTHYFVKIGSADGDLVTLGTTIGRKVLESGVNVTVSQPTLLVNGRTRRFTNIEFQYSTLLNOV7p------------------------------------------------------------NOV7q------------------------------------------------------------NOV7rTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7sTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7t------------------------------------------------------------NOV7uTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKAPDGREGSRLWTEGEKQQLNOV7acTLLLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLNOV7aLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7bLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7c------------------------------------------------------------NOV7d------------------------------------------------------------NOV7e------------------------------------------------------------NOV7f------------------------------------------------------------NOV7g------------------------------------------------------------NOV7h------------------------------------------------------------NOV7i------------------------------------------------------------NOV7j------------------------------------------------------------NOV7k------------------------------------------------------------NOV7lLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7m------------------------------------------------------------NOV7oLSIRYGLTPDTLDEEKARVLDQARQRALGTAWAKEQQKARDGREGSRLWTEGEKQQLLSTNOV7p------------------------------------------------------------NOV7q------------------------------------------------------------NOV7rLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7sLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7t------------------------------------------------------------NOV7uLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7v------------------------------------------------------------NOV7w------------------------------------------------------------NOV7x------------------------------------------------------------NOV7y------------------------------------------------------------NOV7z------------------------------------------------------------NOV7aa------------------------------------------------------------NOV7abLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNENGKR-------------------NOV7acLSTGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKR-------------------NOV7a--------------------------------------NOV7b--------------------------------------NOV7c--------------------------------------NOV7d--------------------------------------NOV7e--------------------------------------NOV7f--------------------------------------NOV7g--------------------------------------NOV7h--------------------------------------NOV7i--------------------------------------NOV7j--------------------------------------NOV7k--------------------------------------NOV7l--------------------------------------NOV7m--------------------------------------NOV7n--------------------------------------NOV7oGRVQGYEGYYVLPVEQYPELADSSSNIQFLRQNEMGKRNOV7p--------------------------------------NOV7q--------------------------------------NOV7r--------------------------------------NOV7s--------------------------------------NOV7t--------------------------------------NOV7u--------------------------------------NOV7v--------------------------------------NOV7w--------------------------------------NOV7x--------------------------------------NOV7y--------------------------------------NOV7z--------------------------------------NOV7aa--------------------------------------NOV7ab--------------------------------------NOV7ac--------------------------------------NOV7a(SEQ ID NO: 114)NOV7b(SEQ ID NO: 116)NOV7c(SEQ ID NO: 118)NOV7d(SEQ ID NO: 120)NOV7e(SEQ ID NO: 122)NOV7f(SEQ ID NO: 124)NOV7g(SEQ ID NO: 126)NOV7h(SEQ ID NO: 128)NOV7i(SEQ ID NO: 130)NOV7j(SEQ ID NO: 132)NOV7k(SEQ ID NO: 134)NOV7l(SEQ ID NO: 136)NOV7m(SEQ ID NO: 138)NOV7n(SEQ ID NO: 140)NOV7o(SEQ ID NO: 142)NOV7p(SEQ ID NO: 144)NOV7q(SEQ ID NO: 146)NOV7r(SEQ ID NO: 148)NOV7s(SEQ ID NO: 150)NOV7t(SEQ ID NO: 152)NOV7u(SEQ ID NO: 154)NOV7v(SEQ ID NO: 156)NOV7w(SEQ ID NO: 158)NOV7x(SEQ ID NO: 160)NOV7y(SEQ ID NO: 162)NOV7z(SEQ ID NO: 164)NOV7aa(SEQ ID NO: 166)NOV7ab(SEQ ID NO: 168)NOV7ac(SEQ ID NO: 170)


Further analysis of the NOV7a protein yielded the following properties shown in Table 7C.

TABLE 7CProtein Sequence Properties NOV7aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 9; pos. chg 4; neg. chg 2H-region:length 3; peak value −8.37PSG score:−12.77GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −14.20possible cleavage site: between 32 and 33>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 380-396−11.73PERIPHERALLikelihood = 3.39 (at 982)ALOM score: −11.73 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 387Charge difference: −6.0 C(0.0)-N(6.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 380)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):3.46Hyd Moment(95):5.35G content:0D/E content:2S/T content:0Score: −7.50Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: RRHR (3) at 6pat7: PRERRSI (5) at 483bipartite: RKEQIKGKVTIFGRKLR at 1712content of basic residues: 9.7%NLS Score: 0.64KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:KKXX-like motif in the C-terminus: EMGKSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 179LL at 200LL at 224LL at 351checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residues•Final Results (k = 9/23):43.5%: nuclear21.7%: mitochondrial13.0%: cytoplasmic 8.7%: Golgi 4.3%: plasma membrane 4.3%: extracellular, including cell wall 4.3%: peroxisomal>> prediction for CG50426-17 is nuc (k = 23)


A search of the NOV7a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 7D.

TABLE 7DGeneseq Results for NOV7aNOV7aResidues/Identities/GeneseqProtein/Organism/LengthMatchSimilarities for theExpectIdentifier[Patent #, Date]ResiduesMatched RegionValueAAU08681Human FCTR3f polypeptide1 . . . 26622620/2728 (96%)0.0sequence - Homo sapiens, 2724 aa.1 . . . 27242628/2728 (96%)[WO200166747-A2, 13-SEP-2001]AAU08680Human FCTR3b polypeptide1 . . . 26622620/2737 (95%)0.0sequence - Homo sapiens, 2733 aa.1 . . . 27332628/2737 (95%)[WO200166747-A2, 13-SEP-2001]ABP53587Human NOV15b protein SEQ ID1 . . . 26621780/2799 (63%)0.0NO: 38 - Homo sapiens, 2721 aa.1 . . . 27212156/2799 (76%)[WO200262999-A2, 15-AUG-2002]ABP53586Human NOV15a protein SEQ ID1 . . . 26621779/2803 (63%)0.0NO: 36 - Homo sapiens, 2725 aa.1 . . . 27252155/2803 (76%)[WO200262999-A2, 15-AUG-2002]ABP53589Human NOV15d protein SEQ ID239 . . . 2662 1689/2546 (66%)0.0NO: 42 - Homo sapiens, 2613 aa.80 . . . 2613 2035/2546 (79%)[WO200262999-A2, 15-AUG-2002]


In a BLAST search of public sequence databases, the NOV7a protein was found to have homology to the proteins shown in the BLASTP data in Table 7E.

TABLE 7EPublic BLASTP Results for NOV7aNOV7aProteinResidues/Identities/AccessionMatchSimilarities for theExpectNumberProtein/Organism/LengthResiduesMatched PortionValueCAC88676Sequence 12 from Patent1 . . . 26622620/2728 (96%)0.0WO0166747 - Homo sapiens1 . . . 27242628/2728 (96%)(Human), 2724 aa.CAC88675Sequence 7 from Patent1 . . . 26622620/2737 (95%)0.0WO0166747 - Homo sapiens1 . . . 27332628/2737 (95%)(Human), 2733 aa.Q9WTS5Ten-m2 - Mus musculus (Mouse),1 . . . 26622582/2768 (93%)0.02764 aa.1 . . . 27642611/2768 (94%)Q9R1K2Neurestin alpha - Rattus1 . . . 26622574/2769 (92%)0.0norvegicus (Rat), 2765 aa.1 . . . 27652610/2769 (93%)Q9ULU2KIAA1127 protein - Homo sapiens590 . . . 2662 2029/2148 (94%)0.0(Human), 2144 aa (fragment).1 . . . 21442037/2148 (94%)


PFam analysis predicts that the NOV7a protein contains the domains shown in the Table 7F.

TABLE 7FDomain Analysis of NOV7aIdentities/SimilaritiesPfamNOV7afor theExpectDomainMatch RegionMatched RegionValueEGF599 . . . 62610/47 (21%)0.4420/47 (43%)EGF631 . . . 65813/47 (28%)0.003720/47 (43%)EGF698 . . . 728 9/47 (19%)0.01924/47 (51%)NHL1302 . . . 133712/36 (33%)0.3627/36 (75%)NHL1431 . . . 145812/29 (41%)0.0007423/29 (79%)


Example 8.

The NOV8 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 8A.

TABLE 8ANOV8 Sequence AnalysisNOV8a, CG50646-04SEQ ID NO: 17711158 bpDNA SequenceORF Start: ATG at 77ORF Stop: TAA at 10787CAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCATGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAAATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGCCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCCTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAATGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8a CG50646-04Protein sequenceSEQ ID NO: 178357O aaMW at 390012.5kDMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAILVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASSWEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTSTVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGSRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFNOV8b, 237582568SEQ ID NO: 1792127 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGGCCGTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGGCGACGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGCTCGCCGGGCATTGCATGAAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGACACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATTACGGGAAAGGTCTGCAGTATGAATGdACAGCTTGCCCATCGGGGACATACAAACCTGAAGCCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAACTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACAGGTACAGACTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGGGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAATAGGACATGTGATATCCATATTGTCATACTCGAGNOV8b, 237582568Protein SequenceSEQ ID NO: 180709 aaMW at 78072.4kDRPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRRRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRPARQHKCALLLQEIPAlSYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALARRALHEDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGTHTGHFECICEKGYYGKGLQYECTACPSGTYKPEASPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVILENOV8c, 236589434SEQ ID NO: 1812127 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGGCCGTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGGCGACGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATCTCTCATCACTGATGGATATTCCAATGGQGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGCTCGCCGGGCATTGCATGAAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGACACACACAGACCGTTTTGAGTGCATCTGTGAAAAGGGGTATTACGGGAAAGGTCTGCAGTATGAATGCACAGCTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAACTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGACTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATATCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTAGGAGTTCAGGCAGCTGTGCGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCCGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGGGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATACTCGAGNOV8c, 236589434Protein SequenceSEQ ID NO: 182709 aaMW at 78142.4kDRPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRRRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPATSYRGGGTYTKGAFQQAAQILLHARENSTKVVSLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALARPALHEDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGTHTDRFECICEKGYYGKGLQYECTACPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDIIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVRKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVILENOV8d, 236495259SEQ ID NO: 1832127 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGGCCGTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGGCGACGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACATCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGCTCGCCGGGCATTGCATGAAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGACACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATTACGGGAAAGGTCTGCAGTATGAATGCACAGCTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGOATACAGGGCATCTGGCCAGACCTGTGAACTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGACTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGCACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGGGCCGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATACTCGAGNOV8d, 236495259Protein sequenceSEQ ID NO: 184709 aaMW at 78070.4kDRPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRRRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAlSYRGGGTYIKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALARRALHEDLPSGSFIQDDMVHCSYLCDEGKDCCDPMGSCKCGTHTGHFECICEKGYYGKGLQYECTACPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERNCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVILENOV8e, 236495256SEQ ID NO: 1852127 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGGCCGTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAACGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGGCGACGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGCCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGGGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAGCAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGCTCGCCGGGCATTGCATGAAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGACACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATTACGGGAAGGGTCTGCAGTATGAATGCACAGCTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAACTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGACTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATATCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTCGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGGGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGAACTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATACTCGAGNOV8e, 236495256Protein SequenceSEQ ID NO: 186709 aaMW at 78081.3kDRPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRRRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRADYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSAKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALARRALHEDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGTHTGHFECICEKGYYGKGLQYECTACPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMWYKTTCLVACDEGYPLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDIIISPHNCGKQPAKFGTICYVSRRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGELFPQGETIVQYTATDPSGNNRTCDIHIVILENOV8f, CG50646-01SEQ ID NO: 1871952 bpDNA SequenceORF Start: ATG at 470ORF Stop: TGA at 1688TTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACACCTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGGGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGCTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACNOV8f, CG50646-01Protein SequenceSEQ ID NO: 188406 aaMW at 44385.5kDMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGSQCLDLFRDRNOV8g, CG50646-02SEQ ID NO: 1891709 bpDNA SequenceORF Start: ATG at 413ORF Stop: TGA at 1445CAGAGTCTTGCTCTGTCTCCCAGGCTGGAGTGCAGTGGCACAATCTCAGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCTTCCCAAATGGCTGAGATTACAGGCACATACCACCATGCCTAGCTAATTTTTGTACAGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTAACCTCAAGTGTTCCTCCTGCCTCCGCCTCCCAAAGTGCTGGGATTGTAGGCATGAATCGTCATGCCGAGCCTAAGTTGACTTTCTACTATCATTTTCACTTATTTAAAAAAATAGAATGGATCTATTGGAAAAACCATAAATCATTATTTGCTTACTTCCTAATTGATTCATTTTAACATAGACCTTTTAGTTTTTTTCACTATCCAAGGATTTAGTTAATGCTATCATCTGTTATACAAATCGCACTCACTTGCTTTCTTCCTGTTGCACAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGGGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCACTCGTGCCCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACNOV8g, CG50646-02Protein SequenceSEQ ID NO: 190344 aaMW at 37763.2kDMLSSVIQIALTCFLPVAQHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGSQCLDLFRDRNOV8h, CG50646-03SEQ ID NO: 1916153 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 6151ATGGCGGGCGCCCCTCCCCCAGCCTCGTTGCCGCCTTGCAGTTTGATCTCAGACTGCTGTGCTAGCAATCAGCGAGATTCCGTGGGCGTAGGACCCTCTGAGCCAGGTGTGGGATATAGTCTCGTGGTGCGCCGTTTCTTAAGCCGGTCTGAAAAGCGCAATATTCGGGTGGGAGTGACCCGATTTTCCAGCTATACTCTTGCTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGTCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGGTCAGTTTTTCTGTAATGAAGGGTTATNGAGCTTTGTTGGGGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAAGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGAATTTGGCTGTGAATCCATCTGGTCCTGGACTTTTCTTGATCAGCAGCAGAGGGTGCAGAACAGCGGATATTGGTGAACCGCAAATGCTGCTGCCTGATCATTCCTCTGGAAGTTTTGTCTCAGAGGAATACCCGGCCATGTGAGGTGTCAGTCCGCCCCTACTGGGGGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAGAAGATATACACAACAGCCCAAGTCCCTGAATTTTCAGCTAGCAGCTTATTGCAGTATTAGAATGTTTATTTTGCGGGGAGGGGTTCAAGATGGCCAACTAGAAACAGCTGTGGCCGGAGCCTCCCACCGAGAAGAACAAAAACAAAAGCGAGAAAAAGCAAGGTGGTACAACGGCCCACCTGGGAGCCACATGGGGCAAGCAGAGCTCCCACCCCCAGCCAAAGGAGGTGGACCTCCCTGCGGGAATTTCAGCAACTCCAGCCAGGGGTTTATGAACAGACCTCTGATCTCCCTGAGATGGAGCCCCTGGGGCTCCATGTGGCCATGGTCTCCACAGAAACAGCACAGTTTACCTGCTCTGCCAAGGGGCAGCTAGAGCGCTTTGTTAAGCGAGTCCCTGATCCCAGAGCTCCCACCCCCAGCCAAAGGAGGTGGACCTCCCTGCGGGAATTTCAGCAACTCCAGCCAGGGGTTTATGAACAGACCTCTGATCTCCCTGAGATGGAGCCCCTGGGGCTCCATGTGGCCATGGTCTCCACAGATCAGCAGGCTTAGTCCTTCCCCTGCTGGCTCTGAGGAATCCAGGCAGGCTGGACTAGTGGGATTCCCCACAGCACAGTTTACCTGCTCTGCCAAGGGGCAGCTAGAGCGCTTTGTTAAGCGAGTCCCTGATCCCATGCCTCCTGATTGGGATGAGACCCCCCCACAACAGGGGTCACGGATGAGACCCCCCCACAACAGGGGTCACCAGACACCTTATACAAGGGTGTTCCTGCTAGCATCAGGTCAGTGCCCCTCTGGGACAGAGCTCCCAGAGGAAAGAGCAGGCAGCCATCTTTGCTGTTCTGCAGCGTCCGCTGGAAAAGCACAGAATTGGGCAGAGGCTAGGATTGATGAATTGAAAGAAGTAGGCTTCAGAAAGTGGGTAATAATGAAGTTCGCTGAGCTAAAGGAACATGTTCTAAACCAATGCAIAGACGCCAAGAACCAGGATAAAACATTACAGGATCCGTTAACCAGAATAACCAGTTTAGAAAGGAATGTAAATGACCTGATGGAGCTGAAAAACACAACACGAGAACTTCACAATGCAACAACAAAACAAGGCCAACATTCFAGTTCAGGAAATCCAGAGAACCCCAGTAAGATACTCCATGAGAAGATCAACCCCAAGACACATAATCCTCAGGTTCTCCAAGAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGGTAGGCCTCTTTCATGGTTTGTTTTCTTGGTGGCTCAGGCCCATGAAACTCCAGAGGACATTGAAGAGTGTGACTTAGACTCAGAAGTGGTGGCAAAATGANOV8h, CG50646-03Protein SequenceSEQ ID NO: 1922050 aaMW at 224498.2kDMAGAPPPASLPPCSLISDCCASNQRDSVGVGPSEPGVGYSLVVRRFLSRSEKRNIRVGVTRFSSYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKGQFFCNEGLXSFVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKERHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRICLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELENLAVNPSGPGLFLVDRTLSCRSELARGPIQTLFAWVSAAEGAEQRILVNRKCCCLIIPLEVLSQRNTRPCEVSVRPYWGGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCERRYTQQPKSLNFQLAAYCSIRMFILRGGVQDGQLETAVAGASHREEQKQKREKARWYNGPPGSHMGQAELPPPAKGGGPPCGNFSNSSQGFMNRPLISLRWSPWGSMWPWSPQISRLSPSPAGSEESRQAGLVGFPTAQFTCSAKGQLERFVKRVPDPMPPDWDETPPQQGSRMRPPHNRGHQTPYTRVFLLASGQCPSGTELPEERAGSHLCCSAASAGKAQNWAEARIDELKEVGFRYWVIMKFAELKEHVLNQCKDAKNQDKTLQDPLTRITSLERNVNDLMELKNTTRELHNATTKQGQHSSSGNPENPSKILHEKINPKTHNPQVLQEISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTGRPLSWFVFLVAQAHETPEDIEECDLDSEVVAKNOV8i, CG50646-05SEQ ID NO: 19311152 bpDNA SequenceORF Start: ATG at 77ORF Stop: TAA at 10781CAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGCTCGCCGGGCATTGCATGAAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAGGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGACACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATTACGGGAAAGGTCTGCAGTATGAATGCACAGCTTGCCCATCGGGGACATACAAACCTGAAGCCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCCGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAACTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGACTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCGCGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGGGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGTAATAACAGGATATGTGATATCCATATTGTCATGAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTACGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAAAAAACGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACTCAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTGAAAGCTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAACCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTGGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGCTGTAATAAAGGATATACTCTGGCCCGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAACTCACCTGTCAGTCAGATGGCCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCGGCCATCTGAGGCACACTGCACAGAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8i, CG50646-05Protein SequenceSEQ ID NO: 1943568 aaMW at 389944.3kDMPRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALARRALHEDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGTHTGHFECICEKGYYGKGLQYECTACPSGTYKPEASPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYPLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVRVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFRIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRICDIHIVMKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCAKKRFANHGFKSFEMFYKAARCDDSDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIRSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTSTVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGSRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILRGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFSEQ ID NO: 19511158 bpNOV8j, SNP13380898 ofORF Start: ATG at 77ORF Stop: TAA at 10787CG50646-04, DNA SequenceSNP Pos: 1068SNP Change: G to CCAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGCCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGACAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTGAAAGCTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAACCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTGGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAATCCGGAAATCCGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGGLAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGALAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCACCATTCTCCGATGCAGTCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8j, SNP13380898 ofCG50646-04, ProteinSEQ ID NO: 196MW at 390026.5kDSequenceSNP Pos: 3313570 aaSNP Change: Gly to AlaMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAlSYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEASPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYFASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYPLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGWNNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSFDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTSTVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGSRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHAITCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFNOV8k, SNP13380899 ofSEQ ID NO: 19711158 bpCG50646-04,ORF Start: ATG at 77ORF Stop: TAA at 10787DNA SequenceSNP Pos: 2404SNP Change: A to GCAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAGCCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTCATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTAGAAACTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTCCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAGGTGTGAAACAGGTATGTATCGTATCTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTGGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAIAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCAAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8k, SNP13380899 ofSEQ ID NO: 198MW at 390012.5kDCG50646-04, Protein SequenceSNP Pos: 7763570 aaSNP Change: Lys to LysMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEAIVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKYYCLEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTSTVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGSRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMNEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFSEQ ID NO: 19911158 bpNOV8l, SNP13374702 ofORF Start: ATG at 77ORF Stop: TAA at 10787CG50646-04, DNA SequenceSNP Pos: 2457SNP Change: A to GCAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCGCGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTGAAAGCTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAACCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTGGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGAGACTTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGACAATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGALAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8l, SNP13374702 ofSEQ ID NO: 200MW at 390031.5kDCG50646-04, Protein SequenceSNP Pos: 7943570 aaSNP Change: His to ArgMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYPASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANRGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCPLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCTECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDNMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKFESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYGYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFSEQ ID NO: 20111158 bpNOV8m, SNP13374257 ofORF Start: ATG at 77ORF Stop: TAA at 10787CG50646-04, DNA SequenceSNP Pos: 7217SNP Change: A to GCAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTGAAAGCTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAACCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAGGTGTGAAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTGGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTGCCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTACATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8m, SNP13374257 ofSEQ ID NO: 202MW at 389982.5kDCG50646-04, Protein SequenceSNP Pos: 23813570 aaSNP Change: Thr to AlaMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYPASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANRGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCPLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCTECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCAPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDNMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKFESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYGYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFSEQ ID NO: 20311158 bpNOV8n, SNP13382479 ofORF Start: ATG at 77ORF Stop: TAA at 10787CG50646-04, DNA SequenceSNP Pos: 9649SNP Change: A to TCAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCCCGCCCGGGGCCCCCCGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGGGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTGGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTGAAAGCTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAACCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAAGTGTGAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAGACCTAGTTGGGGAAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTGGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGAACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAGAGAATCTCCTGCAGTCCTAAAAAATGTCCTCTCCCGGAAAACATAACACATATACTTGTTCATGGGGACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGAAAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGACGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACAAAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8n, SNP13382479 ofSEQ ID NO: 204MW at 390012.5kDCG50646-04, Protein SequenceSNP Pos: 31913570 aaSNP Change: Val to ValMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYFASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYPLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGWNNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSFDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTSTVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGSRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHAITCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWTGHNCSRKRRTGFSEQ ID NO: 20511158 bpNOV8o, SNP13382480 ofORF Start: ATG at 77ORF Stop: TAA at 10787CG50646-04, DNA SequenceSNP Pos: 10749SNP Change: C to TGAATTGGTCTAGGGTCTCCCCCATTGGAATATCCATCAGTGATGAGAAATACAACGTTTGTTGAGTTTTCTCTAGCATGAGAAGAATTTGCGCGGCTTGCTGGGGTCTGGCGCTCGTTTCGGGCTGGGCGACCTTTCAGCAGATGTCCCCGTCGCGCAATTTCAGCTTCCGCCTCTTCCCCGAGACCGCGCCCGGGGCCCCCGGGAGTATCCCCGCGCCGCCCGCTCCTGGCGACGAAGCGGCGGGGAGCAGAGTGGAGCGGCTGGGCCAGGCGTTCCGCGTGCGGCTGCTGCGGGAGCTCAGCGAGCGCCTGGAGCTTGTCTTCCTGGTGGATGATTCGTCCAGCGTGGGCGAAGTCAACTTCCGCAGCGAGCTCATGTTCGTCCGCAAGCTGCTGTCCGACTTCCCCGTGGTGCCCACGGCCACGCGCGTGGCCATCGTGACCTTCTCGTCCAAGAACTACGTGGTGCCGCGCGTCGATTACATCTCCACCCGCCGCGCGCGCCAGCACAAGTGCGCGCTGCTCCTCCAAGAGATCCCTGCCATCTCCTACCGAGGTGGCGGCACCTACACCAAGGGCGCCTTCCAGCAAGCCGCGCAAATTCTTCTTCATGCTAGAGAAAACTCAACAAAAGTTGTATTTCTCATCACTGATGGATATTCCAATGGGGGAGACCCTAGACCAATTGCAGCGTCACTGCGAGATTCAGGAGTGGAGATCTTCACTTTTGGCATATGGCAAGAGAACATTCGAGAGCTGAATGACATGGCTTCCACCCCAAAGGAGGAGCACTGTTACCTGCTACACAGTTTTGAAGAATTTGAGGCTTTAGTCGCCCTCTGTCATATGTTATTTGTAGATCTACCTTCTGGGAGTTTTATTCAAGATGATATGGTCCACTGCTCATATCTTTGTGATGAAGGCAAGGACTGCTGTGACCGAATGGGAAGCTGCAAATGTGGGAAACACACAGGCCATTTTGAGTGCATCTGTGAAAAGGGGTATAACGGGAAAGGTCTGCAGTATGACTGCACAGTTTGCCCATCGGGGACATACAAACCTGAAGGCTCACCAGGAGGAATCAGCAGTTGCATTCCATGTCCTGATGAAAATCACACCTCTCCACCTGGAAGCACATCCCCTGAAGACTGTGTCTGCAGAGAGGGATACAGGGCATCTGGCCAGACCTGTGAAGTTGTCCACTGCCCTGCCCTGAAGCCTCCCGAAAATGGTTACTTTATCCAAAACACTTGCAACAACCACTTCAATGCAGCCTGTGGGGTCCGATGTCACCCTGGATTTGATCTTGTGGGAAGCAGCATCATCTTATGTCTACCCAATGGTTTGTGGTCCGGTTCAGAGAGCTACTGCAGAGTAAGAACATGTCCTCATCTCCGCCAGCCGAAACATGGCCACATCAGCTGTTCTACAAGGGAAATGTTATATAAGACAACATGTTTGGTTGCCTGTGATGAAGGGTACAGGCTAGAAGGCAGTGATAAGCTTACTTGTCAAGGAAACAGCCAGTGGGATGGGCCAGAACCCCGGTGTGTGGAGCGCCACTGTTCCACCTTTCAGATGCCCAAAGATGTCATCATATCCCCCCACAACTGTGGCAAGCAGCCAGCCAAATTTGGGACGATCTGCTATGTAAGTTGCCGCCAAGGGTTCATTTTATCTGGAGTCAAAGAAATGCTGAGATGTACCACTTCTGGAAAATGGAATGTCGGAGTTCAGGCAGCTGTGTGTAAAGACGTGGAGGCTCCTCAAATCAACTGTCCTAAGGACATAGAGGCTAAGACTCTGGAACAGCAAGATTCTGCCAATGTTACCTGGCAGATTCCAACAGCTAAAGACAACTCTGGTGAAAAGGTGTCAGTCCACGTTCATCCAGCTTTCACCCCACCTTACCTTTTCCCAATTGGAGATGTTGCTATCGTATACACGGCAACTGACCTATCCGGCAACCAGGCCAGCTGCATTTTCCATATCAAGGTTATTGATGCAGAACCACCTGTCATAGACTGGTGCAGATCTCCACCTCCCGTCCAGGTCTCGGAGAAGGTACATGCCGCAAGCTGGGATGAGCCTCAGTTCTCAGACAACTCAGGTGCTGAATTGGTCATTACCAGAAGTCATACACAAGGAGACCTTTTCCCTCAAGGGGAGACTATAGTACAGTATACAGCCACTGACCCCTCAGGCAATAACAGGACATGTGATATCCATATTGTCATAAAAGGTTCTCCCTGTGAAATTCCATTCACACCTGTAAATGGGGATTTTATATGCACTCCAGATAATACTGGAGTCAACTGTACATTAACTTGCTTGGAGGGCTATGATTTCACAGAAGGGTCTACTGACAAGTATTATTGTGCTTATGAAGATGGCGTCTGGAAACCAACATATACCACTGAATGGCCAGACTGTGCCAGTAAGCGTTTTGCAAACCACGGGTTCAAGTCCTTTGAGATGTTCTACAAAGCAGCTCGTTGTGATGACACAGATCTGATGAAGAAGTTTTCTGAAGCATTTGAGACGACCCTGGGAAAAATGGTCCCATCATTTTGTAGTGATGCAGAGGACATTGACTGCAGACTGGAGGAGAACCTGACCAAAAAATATTGCCTAGAATATAATTATGACTATGAAAATGGCTTTGCAATTGGTCCAGGTCGCTGGGGTGCAGCTAATAGGCTGGATTACTCTTACGATGACTTCCTGGACACTGTGCAAGAAACAGCCACAAGCATCGGCAATGCCAAGTCCTCACGGATTAAAAGAAGTGCCCCATTATCTGACTATAAAATTAAGTTAATTTTTAACATCACAGCTAGTGTGCCATTACCCGATGAAAGAAATGATACCCTTGAATGGGAAAATCAGCAACGACTCCTTCAGACATTGGAAACTATCACAAATAAACTGAAAAGGACTCTCAACAAAGACCCCATGTATTCCTTTCAGCTTGCATCAGAAATACTTATAGCCGACAGCAATTCATTAGAAACAAAAAAGGCTTCCCCCTTCTGCAGACCAGGCTCAGTGCTGAGAGGGCGTATGTGTGTCAATTGCCCTTTGGGAACCTATTATAATCTGGAACATTTCACCTGTGAAAGCTGCCGGATCGGATCCTATCAAGATGAAGAAGGGCAACTTGAGTGCAAGCTTTGCCCCTCTGGGATGTACACGGAATATATCCATTCAAGAAACATCTCTGATTGTAAAGCTCAGTGTAAACAAGGCACCTACTCATACAGTGGACTTGAGACTTGTGAATCGTGTCCACTGGGCACTTATCAGCCAAAATTTGGTTCCCGGAGCTGCCTCTCGTGTCCAGAAAACACCTCAACTGTGAAAAGAGGAGCCGTGAACATTTCTGCATGTGGAGTTCCTTGTCCAGAAGGAAAATTCTCGCGTTCTGGGTTAATGCCCTGTCACCCATGTCCTCGTGACTATTACCAACCTAATGCAGGGAAGGCCTTCTGCCTGGCCTGTCCCTTTTATGGAACTACCCCATTCGCTGGTTCCAGATCCATCACAGAATGTTCAAGTTTTAGTTCAACTTTCTCAGCGGCAGAGGAAAGTGTGGTGCCCCCTGCCTCTCTTGGACATATTAAAAAGAGGCATGAAATCAGCAGTCAGGCAAGTCATGAATGCTTCTTTAACCCTTGCCACAATAGTGGAACCTGCCAGCAACTTGGGCGTGGTTATGTTTGTCTCTGTCCACTTGGATATACAGGTTTAAGTGTGAAAACAGACATCGATGAGTGCAGCCCACTGCCTTGCCTCAACAATGGAGTTTGTAAAGACCTAGTTGGGGAATTCATTTGTGAGTGCCCATCAGGTTACACAGGTAAGCACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGGTATGTATCAACTCAGTGTTATTAATAACCTTAATAATGCAGTCTGTGAAGACCAGGTTGGGGGATTCTTGTGCAAATGCCCACCTGGATTTTTGGGTACCCGATGTGGAAAGAACGTCGATGAGTGTCTCAGTCAGCCATGCAAAAATGGAGCTACCTGTAAAGACGGTGCCAATAGCTTCAGGTGCCTGTGTGCAGCTGGCTTCACAGGATCACACTGTGAATTGAACATCAATGAATGTCAGTCTAATCCATGTAGAAATCAGGCCACCTGTGTGGATGAATTAAATTCATACAGTTGTAAATGTCAGCCAGGATTTTCAGGCAAAAGGTGTGAAACAGAACAGTCTACAGGCTTTAACCTGGATTTTGAAGTTTCTGGCATCTATGGATATGTCATGCTAGATGGCATGCTCCCATCTCTCCATGCTCTAACCTGTACCTTCTGGATGAAATCCTCTGACGACATGAACTATGGAACACCAATCTCCTATGCAGTTGATAACGGCAGCGACAATACCTTGCTCCTGACTGATTATAACGGGTGGGTTCTTTATGTGAATGGCAGGGAAAAGATAACAAACTGTCCCTCGGTGAATGATGGCAGATGGCATCATATTGCAATCACTTCGACAAGTACTGGTGGAGCCTGGAGGGTCTATATAAATGGGGAATTATCTGACGGTGGTACTGGCCTCTCCATTGGCAAAGCCATACCTGGTGGCGGTGCATTAGTTCTTGGGCAAGAGCAAGACAAAAAAGGAGAGGGGTTCAACCCGGCTGAGTCTTTTGTGGGCTCCATAAGCCAGCTCAACCTCTGGGACTATGTCCTGTCTCCACAGCAGGTGAAGTCACTGGCTACCTCCTGCCCAGAGGAACTCAGTAAAGGAAACGTGTTAGCATGGCCTGATTTCTTGTCAGGAATTGTGGGGAAAGTGAAGATCGATTCTAAGAGCATATTTTGTTCTGATTGCCCACGCTTGGGAGGGTCAGTGCCTCATCTGAGAACTGCATCTGAAGATTTAAAACCAGGTTCCAAAGTCAATCTGTTCTGTGAACCAGGCTTCCAGCTGGTCGGGAACCCTGTGCAGTACTGTCTGAATCAAGGACAGTGGACACAACCACTCCCCCACTGTGAACGCATTCGCTGTGGGGTGCCACCTCCTTTGGAGAATGGCTTCCATTCAGCCGATGACTTCTATGCTGGCAGCACAGTAACCTACCAGTGCAACAATGGCTACTATCTATTGGGTGACTCAAGGATGTTCTGTACAGATAATGGGAGCTGGGACGGCGTTTCACCATCCTGCTTAGATGTCGATGAGTGTGCAGTTGGATCAGATTGTAGTGAGCATGCTTCTTGCCTGAACGTAGATGGATCCTACATATGTTCATGTGTCCCACCGTACACAGGAGATGGGAAAAACTGTGCAGAACCTATAAAATGTAAGGCTCCAGGAAATCCGGAAAATGGCCACTCCTCAGGTGAGATTTATACAGTAGGTGCCGAAGTCACATTTTCGTGTCAGGAAGGATACCAGTTGATGGGAGTAACCAAAATCACATGTTTGGAGTCTGGAGAATGGAATCATCTAATACCATATTGTAAAGCTGTTTCATGTGGTAAACCGGCTATTCCAGAAAATGGTTGCATTGAGGAGTTAGCATTTACTTTTGGCAGCAAAGTGACATATAGGTGTAATAAAGGATATACTCTGGCCGGTGATAAAGAATCATCCTGTCTTGCTAACAGTTCTTGGAGTCATTCCCCTCCTGTGTGTGAACCAGTGAAGTGTTCTAGTCCGGAAAATATAAATAATGGAAAATATATTTTGAGTGGGCTTACCTACCTTTCTACTGCATCATATTCATGCGATACAGGATACAGCTTACAGGGCCCTTCCATTATTGAATGCACGGCTTCTGGCATCTGGGACAGAGCGCCACCTGCCTGTCACCTCGTCTTCTGTGGAGAACCACCTGCCATCAAAGATGCTGTCATTACGGGGAATAACTTCACTTTCAGGAACACCGTCACTTACACTTGCAAAGAAGGCTATACTCTTGCTGGTCTTGACACCATTGAATGCCTGGCCGACGGCAAGTGGAGTAGAAGTGACCAGCAGTGCCTGGCTGTCTCCTGTGATGAGCCACCCATTGTGGACCACGCCTCTCCAGAGACTGCCCATCGGCTCTTTGGAGACATTGCATTCTACTACTGCTCTGATGGTTACAGCCTAGCAGACAATTCCCAGCTTCTCTGCAATGCCCAGGGCAAGTGGGTACCCCCAGAAGGTCAAGACATGCCCCGTTGTATAGCTCATTTCTGTGAAAAACCTCCATCGGTTTCCTATAGCATCTTGGAATCTGTGAGCAAAGCAAAATTTGCAGCTGGCTCAGTTGTGAGCTTTAAATGCATGGAAGGCTTTGTACTGAACACCTCAGCAAAGATTGAATGTATGAGAGGTGGGCAGTGGAACCCTTCCCCCATGTCCATCCAGTGCATCCCTGTGCGGTGTGGAGAGCCACCAAGCATCATGAATGGCTATGCAAGTGGATCAAACTACAGTTTTGGAGCCATGGTGGCTTACAGCTGCAACAAGGGGTTCTACATCAAAGGGGAAAAGAAGAGCACCTGCGAAGCCACAGGGCAGTGGAGTAGTCCTATACCGACGTGCCACCCGGTATCTTGTGGTGAACCACCTAAGGTTGAGAATGGCTTTCTGGAGCATACAACTGGCAGGATCTTTGAGAGTGAAGTGAGGTATCAGTGTAACCCGGGCTATAAGTCAGTCGGAAGTCCTGTATTTGTCTGCCAAGCCAATCGCCACTGGCACAGTGAATCCCCTCTGATGTGTGTTCCTCTCGACTGTGGAAAACCTCCCCCGATCCAGAATGGCTTCATGAAAGGAGAAAACTTTGAAGTAGGGTCCAAGGTTCAGTTTTTCTGTAATGAGGGTTATGAGCTTGTTGGTGACAGTTCTTGGACATGTCAGAAATCTGGCAAATGGAATAAGAAGTCAAATCCAAAGTGCATGCCTGCCAAGTGCCCAGAGCCGCCCCTCTTGGAAAACCAGCTAGTATTAAAGGAGTTGACCACCGAGGTAGGAGTTGTGACATTTTCCTGTAAAGAAGGGCATGTCCTGCAAGGCCCCTCTGTCCTGAAATGCTTGCCATCCCAGCAATGGAATGACTCTTTCCCTGTTTGTAAGATTGTTCTTTGTACCCCACCTCCCCTAATTTCCTTTGGTGTCCCCATTCCTTCTTCTGCTCTTCATTTTGGAAGTACTGTCAAGTATTCTTGTGTAGGTGGGTTTTTCCTAAGAGGAAATTCTACCACCCTCTGCCAACCTGATGGCACCTGGAGCTCTCCACTGCCAGAATGTGTTCCAGTAGAATGTCCCCAACCTGAGGAAATCCCCAATGGAATCATTGATGTGCAAGGCCTTGCCTATCTCAGCACAGCTCTCTATACCTGCAAGCCAGGCTTTGAATTGGTGGGAAATACTACCACCCTTTGTGGAGAAAATGGTCACTGGCTTGGAGGAAAACCAACATGTAAAGCCATTGAGTGCCTGAAACCCAAGGAGATTTTGAATGGCAAATTCTCTTACACGGACCTACACTATGGACAGACCGTTACCTACTCTTGCAACCGAGGCTTTCGGCTCGAAGGTCCCAGTGCCTTGACCTGTTTAGAGACAGGTGATTGGGATGTAGATGCCCCATCTTGCAATGCCATCCACTGTGATTCCCCACAACCCATTGAAAATGGTTTTGTAGAAGGTGCAGATTACAGCTATGGTGCCATAATCATCTACAGTTGCTTCCCTGGGTTTCAGGTGGCTGGTCATGCCATGCAGACCTGTGAAGAGTCAGGATGGTCAAGTTCCATCCCAACATGTATGCCAATAGACTGTGGCCTCCCTCCTCATATAGATTTTGGAGACTGTACTAAACTCAAAGATGACCAGGGATATTTTGAGCAAGAAGACGACATGATGGAAGTTCCATATGTGACTCCTCACCCTCCTTATCATTTGGGAGCAGTGGCTAAAACCTGGGAAAATACAAAGGAGTCTCCTGCTACACATTCATCAAACTTTCTGTATGGTACCATGGTTTCATACACCTGTAATCCAGGATATGAACTTCTGGGGAACCCTGTGCTGATCTGCCAGGAAGATGGAACTTGGAATGGCAGTGCACCATCCTGCATTTCAATTGAATGTGACTTGCCTACTGCTCCTGAAAATGGCTTTTTGCGTTTTACAGAGACTAGCATGGGAAGTGCTGTGCAGTATAGCTGTAAACCTGGACACATTCTAGCAGGCTCTGACTTAAGGCTTTGTCTAGAGAATAGAAAGTGGAGTGGTGCCTCCCCACGCTGTGAAGCCATTTCATGCAAAAAGCCAAATCCAGTCATGAATGGATCCATCAAAGGAAGCAACTACACATACCTGAGCACGTTGTACTATGAGTGTGACCCCGGATATGTGCTGAATGGCACTGAGAGGAGAACATGCCAGGATGACAAAAACTGGGATGAGGATGAGCCCATTTGCATTCCTGTGGACTGCAGTTCACCCCCAGTCTCAGCCAATGGCCAGGTGAGAGGAGACGAGTACACATTCCAAAAAGAGATTGAATACACTTGCAATGAAGGGTTCTTGCTTGAGGGAGCCAGGAGTCGGGTTTGTCTTGCCAATGGAAGTTGGAGTGGAGCCACTCCCGACTGTGTGCCTGTCAGATGTGCCACCCCGCCACAACTGGCCAATGGGGTGACGGAAGGCCTGGACTATGGCTTCATGAAGGAAGTAACATTCCACTGTCACGAGGGCTACATCTTGCACGGTGCTCCAAAACTCACCTGTCAGTCAGATGGCAACTGGGATGCAGAGATTCCTCTCTGTAAACCAGTCAACTGTGGACCTCCTGAAGATCTTGCCCATGGTTTCCCTAATGGTTTTTCCTTTATTCATGGGGGCCATATACAGTATCAGTGCTTTCCTGGTTATAAGCTCCATGGAAATTCATCAAGAAGGTGCCTCTCCAATGGCTCCTGGAGTGGCAGCTCACCTTCCTGCCTGCCTTGCAGATGTTCCACACCAGTAATTGAATATGGAACTGTCAATGGGACAGATTTTGACTGTGGAAAGGCAGCCCGGATTCAGTGCTTCAAAGGCTTCAAGCTCCTAGGACTTTCTGAAATCACCTGTGAAGCCGATGGCCAGTGGAGCTCTGGGTTCCCCCACTGTGAACACACTTCTTGTGGTTCTCTTCCAATGATACCAAATGCGTTCATCAGTGAGACCAGCTCTTGGAAGGAAAATGTGATAACTTACAGCTGCAGGTCTGGATATGTCATACAAGGCAGTTCAGATCTGATTTGTACAGAGAAAGGGGTATGGAGCCAGCCTTATCCAGTCTGTGAGCCCTTGTCCTGTGGGTCCCCACCGTCTGTCGCCAATGCAGTGGCAACTGGAGAGGCACACACCTATGAAAGTGAAGTGAAACTCAGATGTCTGGAAGGTTATACGATGGATACAGATACAGATACATTCACCTGTCAGAAAGATGGTCGCTGGTTCCCTGAACGATTTCAGTGTGAATAGGCAAGTTTCTGTGTCATGTGCAGAAGGGTATACCTTTGAGGGAGTTAACATATCAGTATGTCAGCTTGATGGAACCTGGGAGCCACCATTCTCCGATGAATCTTGCAGTCCAGTTTCTTGTGGGAAACCTGAAAGTCCAGAACATGGATTTGTGGTTGGCAGTAAATACACCTTTGAAAGCACAATTATTTATCAGTGTGAGCCTGGCTATGAACTAGAGGGGAACAGGGAACGTGTCTGCCAGGAGAACAGACAGTGGAGTGGAGGGGTGGCAATATGCAAAGAGACCAGGTGTGAAACTCCACTTGAATTTCTCAATGGGAAAGCTGACATTGAAAACAGGACGACTGGACCCAACGTGGTATATTCCTGCAACAGAGGCTACAGTCTTGAAGGGCCATCTGAGGCACACTGCACAGALAATGGAACCTGGAGCCACCCAGTCCCTCTCTGCAAACCAAATCCATGCCCTGTTCCTTTTGTGATTCCCGAGAATGCTCTGCTGTCTGAAAAGGAGTTTTATGTTGATCAGAATGTGTCCATCAAATGTAGGGAAGGTTTTCTGCTGCAGGGCCACGGCATCATTACCTGCAACCCCGACGAGACGTGGACACAGACAAGCGCCAAATGTGAAAAAATCTCATGTGGTCCACCAGCTCACGTAGAAAATGCAATTGCTCGAGGCGTACATTATCAATATGGAGACATGATCACCTACTCATGTTACAGTGGATACATGTTGGAGGGTTTCCTGAGGAGTGTTTGTTTAGAAAATGGAACATGGACATCACCTCCTATTTGCAGAGCTGTCTGTCGATTTCCATGTCAGAATGGGGGCATCTGCCAACGCCCAAATGCTTGTTCCTGTCCAGAGGGCTGGATGGGGCGCCTCTGTGAAGAACCAATCTGCATTCTTCCCTGTCTGAACGGAGGTCGCTGTGTGGCCCCTTACCAGTGTGACTGCCCGCCTGGCTGGACGGGGTCTCGCTGTCATACAGCTGTTTGCCAGTCTCCCTGCTTAAATGGTGGAAAATGTGTAAGACCAAACCGATGTCACTGTCTTTCTTCTTGGATGGGACATAACTGTTCCAGGAAAAGGAGGACTGGGTTTTAACCACTGCACGACCATCTGGCTCTCCCAAAAGCAGGATCATCTCTCCTCGGTAGTGCCTGGGCATCCTGGAACTTATGCAAAGAAAGTCCAACATGGTGCTGGGTCTTGTTTAGTAAACTTGTTACTTGGGGTTACTTTTTTTATTTTGTGATATATTTTGTTATTCCTTGTGACATACTTTCTTACATGTTTCCATTTTTAAATATGCCTGTATTTTCTATATAAAAATTATATTAAATAGATGCTGCTCTACCCTCACALAATGTACATATTCTGCTGTCTATTGGGAAAGTTCCTGGTACACATTTTTATTCAGTTACTTAAAATGATTTTTCCATTAAAGTATATTTTGCTACTAAATAAAAAAAANOV8o, SNP13382480 ofSEQ ID NO: 206MW at 390042.6kDCG50646-04, Protein SequenceSNP Pos: 35583570 aaSNP Change: Thr to MetMRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVERLGQAFRVRLLRELSERLELVFLVDDSSSVGEVNFRSELMFVRKLLSDFPVVPTATRVAIVTFSSKNYVVPRVDYISTRRARQHKCALLLQEIPAlSYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGVEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDMVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKGLQYDCTVCPSGTYKPEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYFASGQTCEVVHCPALKPPENGYFIQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHISCSTREMLYKTTCLVACDEGYPLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIISPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGWNNVGVQAAVCKDVEAPQINCPKDIEAKTLEQQDSANVTWQIPTAKDNSGEKVSVHVHPAFTPPYLFPIGDVAIVYTATDLSGNQASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWDEPQFSDNSGAELVITRSHTQGDLFPQGETIVQYTATDPSGNNRTCDIHIVIKGSPCEIPFTPVNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCLEYNYDYENGFAIGPGGWGAANRLDYSFDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKIKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILIADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKLCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTSTVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGSRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRGYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCPPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHAITCTFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHIAITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRLGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPPPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSDCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFSCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTGYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMEGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNKGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGYELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPSALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHCLSSWMGHNCSRKRRTGF


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 8B.

TABLE 8BComparison of the NOV8 protein sequences.NOV8a MRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVNOV8b --------------RPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVNOV8c --------------RPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVNOV8d --------------RPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVNOV8e --------------RPWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVNOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h --------------MAGAPPPASLPPCSLISDCCASNQRDSVGVGPSEPGVGYSLVVRRFNOV8i MRRICAACWGLALVSGWATFQQMSPSRNFSFRLFPETAPGAPGSIPAPPAPGDEAAGSRVNOV8a ERLGQAFR--VRLLRELSERLELVFLVDDSSS----------------------------NOV8b ERLGQAFRRRVRLLRELSERLELVFLVDDSSS----------------------------NOV8c ERLGQAFRRRVRLLRELSERLELVFLVDDSSS----------------------------NOV8d ERLGQAFRRRVRLLRELSERLELVFLVDDSSS----------------------------NOV8e ERLGQAFRRRVRLLRELSERLELVFLVDDSSS----------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h LSRSEKRNIRVGVTRFSSYTLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETNOV8i ERLGQAFR--VRLLRELSERLELVFLVDDSSS----------------------------NOV8a ----------------------------VGEVNFRSELMFVRKLLSDFPVVPTATRVAIVNOV8b ----------------------------VGEVNFRSELMFVRKLLSDFPVVPTATRVAIVNOV8c ----------------------------VGEVNFRSELMFVRKLLSDFPVVPTATRVAIVNOV8d ----------------------------VGEVNFRSELMFVRKLLSDFPVVPTATRVAIVNOV8e ----------------------------VGEVNFRSELMFVRKLLSDFPVVPTATRVAIVNOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h AHRLFGDIAFYYCSDGYSLADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILNOV8i ----------------------------VGEVNFRSELMFVRKLLSDFPVVPTATRVAIVNOV8a TFSSKN------------------------------YVVPRVDYISTRRARQHKCALLLQNOV8b TFSSKN------------------------------YVVPRVDYISTPRARQHKCALLLQNOV8c TFSSKN------------------------------YVVPRVDYISTRPARQHKCALLLQNOV8d TFSSKN------------------------------YVVPRVDYISTRRARQHKCALLLQNOV8e TFSSKN------------------------------YVVPRADYISTRRARQHKCALLLQNOV8f ------------------------------------------MSIQCIPVRCGEPPSINNNOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i TFSSKN------------------------------YVVPRVDYISTRRARQHKCALLLQNOV8a EIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGNOV8b EIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGNOV8c EIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGNOV8d EIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGNOV8e EIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGNOV8f GYASGSNYSFGAMVAYSCNKGFYIKGEKYSTCEATGQWSSPIPTCHPVSCGEPPKVENGFNOV8g --------------------------------------------MLSSVIQIALTCFLPVNov8h GYASGSNYSFGAMVAYSCNKGFYIKGEKYSTCEATGQWSSPIPTCHPVSCGEPPKVENGFNOV8i EIPAISYRGGGTYTKGAFQQAAQILLHARENSTKVVFLITDGYSNGGDPRPIAASLRDSGNOV8a VEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALVALCHMLFVDLPSGSFIQDDNOV8b VEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALAR--RALHEDLPSGSFIQDDNOV8c VEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALAR--RALHEDLPSGSFIQDDNOV8d VEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALAR--RALHEDLPSGSFIQDDNOV8e VEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALAR--RALHEDLPSGSFIQDDNOV8f LEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESP-LMCVPLDCGKPPPIQNGNOV8g AQHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESP-LMCVPLDCGKPPPIQNGNOV8h LEHTTGRIFESEVRYQCNPGYKSVGSPVFVCQANRHWHSESP-LMCVPLDCGKPPPIQNGN0V8i VEIFTFGIWQGNIRELNDMASTPKEEHCYLLHSFEEFEALAR--RALHEDLPSGSFIQDDNOV8a MVHCSYLCDEGKDCCDRMGSCKCGKHTGHFECICEKGYNGKG----LQYDCTVCPSGTYKNOV8b MVHCSYLCDEGKDCCDRMGSCKCGTHTGHFECICEKGYNGKG----LQYECTACPSGTYKNOV8c MVHCSYLCDEGKDCCDRMGSCKCGTHTDRFECICEKGYYGKG----LQYECTACPSGTYKNOV8d MVHCSYLCDEGKDCCDRMGSCKCGTHTGHFECICEKGYYGKG----LQYECTACPSGTYKNOV8e MVHCSYLCDEGKDCCDRNGSCKCGTHTGHFECICEKGYYGKG----LQYECTACPSGTYKNOV8f FMKGENFEVGSKVQFFCNEGY-ELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQNOV8g FMKGENFEVGSKVQFFCNEGY-ELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQNOV8h FMKGENFEVGSKGQFFCNEGLXSFVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQNOV8i MVHCSYLCDEGKDCCDRMGSCKCGTHTGHFECICEKGYYGKG----LQYECTACPSGTYKNOV8a PEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCEVVHCPALKPPENGYFNOV8b PEASPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYPASGQTCELVHCPALKPPENGYFNOV8c PEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPAWKPPENGYFNOV8d PEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFNOV8e PEGSPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFNOV8f LVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPNOV8g LVLKELTTEVGVVTFSCKEGHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPNOV8h LVLKELTTEVGVVTFSCKERHVLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPNOV8i PEASPGGISSCIPCPDENHTSPPGSTSPEDCVCREGYRASGQTCELVHCPALKPPENGYFNOV8a IQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHINOVBb IQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHINOV8c IQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHINOV8d IQNTCNNHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHINOV8e IQNTCNMHFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHINOV8f IP-SSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIINOV8g IP-SSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEETPNGIINOV8h IP-SSALHFGSTVKYSCVGGFFLRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIINOV8i IQNTCNNEFNAACGVRCHPGFDLVGSSIILCLPNGLWSGSESYCRVRTCPHLRQPKHGHINOV8a SCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIINOV8b SCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIINOV8c SCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDIIINOV8d SCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIINOV8e SCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDIIINOV8f DVQG--LAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKP---T--CKAIECLKPKEINOV8g DVQG--LAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKP---T--CKAIECLKPKEINOV8h DVQG--LAYLSTALYTCKPGFELVGNTTTLCGENGHWLGGKP---T--CKAIECLKPKEINOV8i SCSTREMLYKTTCLVACDEGYRLEGSDKLTCQGNSQWDGPEPRCVERHCSTFQMPKDVIINOV8a SPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCNOV8b SPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCNOV8c SPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCNOV8d SPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCNOV8e SPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCNOV8f LNGKFSYTDLHYGQTVTYSCNRGFRLEGS-QCLDLFRDR---------------------NOV8g LNGKFSYTDLHYGQTVTYSCNRGFRLEGS-QCLDLFRDR---------------------NOV8h LNGKFSYTDLHYGQTVTYSCNRGFRLEGP-SALTCLETGDWDVDAPSCNAIHCDSPQPIENOV8i SPHNCGKQPAKFGTICYVSCRQGFILSGVKEMLRCTTSGKWNVGVQAAVCKDVEAPQINCNOV8a PKDIEAKTLEQ-------------------------------------------------NOV8b PKDIEAKTLEQ-------------------------------------------------NOV8c PKDIEAKTLEQ-------------------------------------------------NOV8d PKDIEAKTLEQ-------------------------------------------------NOV8e PKDIEAKTLEQ-------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h NGFVEGADYSYGAIIIYSCFPGFQVAGHAMQTCEESGWSSSIPTCMPIDCGLPPHIDFGDNOV8i PKDIEAKTLEQ-------------------------------------------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h CTKLKDDQGYFEQEDDMMEVPYVTPHPPYHLGAVAKTWENTKESPATHSSNFLYGTMVSYNOV8i ------------------------------------------------------------NOV8a --------------------------QDSANVTWQIPTAKDN-----SGEKVSVHVHPAFNOV8b --------------------------QDSANVTWQIPTAKDN-----SGEKVSVHVHPAFNOV8c --------------------------QDSANVTWQIPTAKDN-----SGEKVSVHVHPAFNOV8d --------------------------QDSANVTWQIPTAKDN-----SGEKVSVHVHPAFNOV8e --------------------------QDSANVTWQIPTAKDN-----SGEKVSVHVHPAFNOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h TCNPGYELLGNPVLICQEDGTWNGSAPSCISIECDLPTAPENGFLRFTETSMGSAVQYSCNOV8i --------------------------QDSANVTWQIPTAKDN-----SGEKVSVHVHPAFNOV8a TPPYLFPIGDVAIVYTATDLSGN-------------------------------------NOV8b TPPYLFPIGDVAIVYTATDLSGN-------------------------------------NOV8c TPPYLFPIGDVAIVYTATDLSGN-------------------------------------NOV8d TPPYLFPIGDVAIVYTATDLSGN-------------------------------------NOV8e TPPYLFPIGDVAIVYTATDLSGN-------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h KPGHILAGSDLRLCLENRXWSGASPRCEAISCKKPNPVMNGSIKGSNYTYLSTLYYECDPNOV8i TPPYLFPIGDVAIVYTATDLSGN-------------------------------------NOV8a --------QASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWD-------------NOV8b --------QASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWD-------------NOV8c --------QASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWD-------------NOV8d --------QASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWD-------------NOV8e --------QASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWD-------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOv8h GYVLNGTERRTCQDDKNWDEDEPICIPVDCSSPPVSANGQVRGDEYTFQKEIEYTCNEGFNOV8i --------QASCIFHIKVIDAEPPVIDWCRSPPPVQVSEKVHAASWD-------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h LLEGARSRVCLANGSWSGATPDCVPVRCATPPQLANGVTEGLDYGFMKEVTFHCHEGYILNOV8i ------------------------------------------------------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h HGAPKLTCQSDGNWDAEIPLCKPVNCGPPEDLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNOV8i ------------------------------------------------------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h NSSRRCLSNGSWSGSSPSCLPCRCSTPVIEYGTVNGTDFDCGKAARIQCFKGFKLLGLSENOV8i ------------------------------------------------------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ITCEADGQWSSGFPHCEHTSCGSLPMIPNAFISETSSWKENVITYSCRSGYVIQGSSDLINOV8i ------------------------------------------------------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h CTEKGVWSQPYPVCEPLSCGSPPSVANAVATGEAHTYESEVKLRCLEGYTMDTDTDTFTCNOV8i ------------------------------------------------------------NOV8a ------------------------------------------------------------NOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h QKDGRWFPERISCSPKKCPLPENITHILVHGDDFSVNRQVSVSCAEGYTFEGVNISVCQLNOV8i ------------------------------------------------------------NOV8a ----EPQFSDNSGAELVITRSHTQGDLFPQGE---------------TIVQYTATDPSGNNOV8b ----EPQFSDNSGAELVITRSHTQGDLFPQGE---------------TIVQYTATDPSGNNOV8c ----EPQFSDNSGAELVITRSHTQGDLFPQGE---------------TIVQYTATDPSGNNOV8d ----EPQFSDNSGAELVITRSHTQGDLFPQGE---------------TIVQYTATDPSGNNOV8e ----EPQFSDNSGAELVITRSHTQGELFPQGE---------------TIVQYTATDPSGNNOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h DGTWEPPFSDESCSPVSCGKPESPEHGFVVGSKYTFESTIIYQCEPGYELENLAVNPSGPNOV8i ----EPQFSDNSGAELVITRSHTQGDLFPQGE---------------TIVQYTATDPSGNNOV8a ---------------------------------------NRTCDIHIVIKGSPCEIPFTPNOV8b ---------------------------------------NRTCDIHIVILE---------NOV8c ---------------------------------------NRTCDIHIVILE---------NOV8d ---------------------------------------NRTCDIHIVILE---------NOV8e ---------------------------------------NRTCDIHIVILE---------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h GLFLVDRTLSCRSELARGPIQTLFAWVSAAEGAEQRTLVNRKCCCLIIPLEVLSQRNTRPNOV8i ---------------------------------------NRICDIHIVMKGSPCEIPFTPNOV8a VNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h CEVSVRPYWGGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENRTTGPNVVYSNOV8i VNGDFICTPDNTGVNCTLTCLEGYDFTEGSTDKYYCAYEDGVWKPTYTTEWPDCASKRFANOV8a NHGFKSFEMFYKAARCDDTDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h CNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKEFYVDQNVSIKCNOV8i NHGFKSFEMFYKAARCDDSDLMKKFSEAFETTLGKMVPSFCSDAEDIDCRLEENLTKKYCNOV8a LEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h REGFLLQGHGIITCNPDETWTQTSAKCERRYTQQPKSLNFQLAAYCSIRMFILRGGVQDGNOV8i LEYNYDYENGFAIGPGGWGAANRLDYSYDDFLDTVQETATSIGNAKSSRIKRSAPLSDYKNOV8a IKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILINOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h QLETAVAGASHREEQKQKREKARWYNGPPGSHMGQAELPPPAKGGGPPCGNFSNSSQGFMNOV8i IKLIFNITASVPLPDERNDTLEWENQQRLLQTLETITNKLKRTLNKDPMYSFQLASEILINOV8a ADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h NRPLISLRWSPWGSMWPWSPQISRLSPSPAGSEESRQAGLVGFPTAQFTdSAKGQLERFVNOV8i ADSNSLETKKASPFCRPGSVLRGRMCVNCPLGTYYNLEHFTCESCRIGSYQDEEGQLECKNOV8a LCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTYQPKFGSRSCLSCPENTNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h KRVPDPMPPDWDETPPQQGSRMRPPHNRGHQTPYTRVFLLASGQCPSGTELPEERAGSHLNOV8i LCPSGMYTEYIHSRNISDCKAQCKQGTYSYSGLETCESCPLGTVQPKFGSRSCLSCPENTNOV8a STVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h CCSAASAGKAQNWAEARIDELKEVGFRKWVIMKFAELKEHVLNQCKDAKNQDKTLQDPLTNOV8i STVKRGAVNISACGVPCPEGKFSRSGLMPCHPCPRDYYQPNAGKAFCLACPFYGTTPFAGNOV8a SRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h RITSLERNVNDLMELKNTTRELHNATTKQGQHSSSGNPENPSKILHEKINPKTHNPQVLQNOV8i SRSITECSSFSSTFSAAEESVVPPASLGHIKKRHEISSQASHECFFNPCHNSGTCQQLGRNOV8a GYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h EISCGPPAHVENAIARGVHYQYGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCNOV8i GYVCLCPLGYTGLKCETDIDECSPLPCLNNGVCKDLVGEFICECPSGYTGKHCELNINECNOV8a QSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h RFPCQNGGICQRPNACSCPEGWMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHNov8i QSNPCRNQATCVDELNSYSCKCQPGFSGKRCETGMYQLSVINNLNNAVCEDQVGGFLCKCNOV8a PPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h TGRPLSWFVFLVAQAHETPEDIEECDLDSEVVAK--------------------------NOV8i PPGFLGTRCGKNVDECLSQPCKNGATCKDGANSFRCLCAAGFTGSHCELNINECQSNPCRNOV8a NQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i NQATCVDELNSYSCKCQPGFSGKRCETEQSTGFNLDFEVSGIYGYVMLDGMLPSLHALTCNOV8a TFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHINOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i TFWMKSSDDMNYGTPISYAVDNGSDNTLLLTDYNGWVLYVNGREKITNCPSVNDGRWHHINOV8a AITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i AITWTSTGGAWRVYINGELSDGGTGLSIGKAIPGGGALVLGQEQDKKGEGFNPAESFVGSNOV8a ISQLNLWDYVLSPQQVKSLATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i ISQLNLWDYVLSPQQVKSIATSCPEELSKGNVLAWPDFLSGIVGKVKIDSKSIFCSDCPRNOV8a LGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i LGGSVPHLRTASEDLKPGSKVNLFCEPGFQLVGNPVQYCLNQGQWTQPLPHCERIRCGVPNOV8a PPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSPMFCTDNGSWNGVSPSCLDVDECAVGSNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i PPLENGFHSADDFYAGSTVTYQCNNGYYLLGDSRMFCTDNGSWNGVSPSCLDVDECAVGSNOV8a DCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i DCSEHASCLNVDGSYICSCVPPYTGDGKNCAEPIKCKAPGNPENGHSSGEIYTVGAEVTFNOV8a SCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPAIPENGCIEELAFTFGSKVTYRCNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i SCQEGYQLMGVTKITCLESGEWNHLIPYCKAVSCGKPATPENGCIEELAFTFGSKVTYRCNOV8a NKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i NKGYTLAGDKESSCLANSSWSHSPPVCEPVKCSSPENINNGKYILSGLTYLSTASYSCDTNOV8a GYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i GYSLQGPSIIECTASGIWDRAPPACHLVFCGEPPAIKDAVITGNNFTFRNTVTYTCKEGYNOV8a TLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i TLAGLDTIECLADGKWSRSDQQCLAVSCDEPPIVDHASPETAHRLFGDIAFYYCSDGYSLNOV8a ADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i ADNSQLLCNAQGKWVPPEGQDMPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFKCMNOV8a EGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i EGFVLNTSAKIECMRGGQWNPSPMSIQCIPVRCGEPPSIMNGYASGSNYSFGAMVAYSCNNOV8a KGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i KGFYIKGEKKSTCEATGQWSSPIPTCHPVSCGEPPKVENGFLEHTTGRIFESEVRYQCNPNOV8a GYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i GYKSVGSPVFVCQANRHWHSESPLMCVPLDCGKPPPIQNGFMKGENFEVGSKVQFFCNEGNOV8a YELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i YELVGDSSWTCQKSGKWNKKSNPKCMPAKCPEPPLLENQLVLKELTTEVGVVTFSCKEGHNOV8a VLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i VLQGPSVLKCLPSQQWNDSFPVCKIVLCTPPPLISFGVPIPSSALHFGSTVKYSCVGGFFNOV8a LRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i LRGNSTTLCQPDGTWSSPLPECVPVECPQPEEIPNGIIDVQGLAYLSTALYTCKPGFELVNOV8a GNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i GNTTTLCGENGHWLGGKPTCKAIECLKPKEILNGKFSYTDLHYGQTVTYSCNRGFRLEGPNOV8a SALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i SALTCLETGDWDVDAPSCNAIHCDSPQPIENGFVEGADYSYGAIIIYSCFPGFQVAGHAMNOV8a QTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i QTCEESGWSSSIPTCMPIDCGLPPHIDFGDCTKLKDDQGYFEQEDDMMEVPYVTPHPPYHNOV8a LGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCINOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i LGAVAKTWENTKESPATHSSNFLYGTMVSYTCNPGYELLGNPVLICQEDGTWNGSAPSCINOV8a SIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAINOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i SIECDLPTAPENGFLRFTETSMGSAVQYSCKPGHILAGSDLRLCLENRKWSGASPRCEAINOV8a SCKKPNPVNNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i SCKKPNPVMNGSIKGSNYTYLSTLYYECDPGYVLNGTERRTCQDDKNWDEDEPICIPVDCNOV8a SSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i SSPPVSANGQVRGDEYTFQKEIEYTCNEGFLLEGARSRVCLANGSWSGATPDCVPVRCATNOV8a PPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPENOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i PPQLANGVTEGLDYGFMKEVTFHCHEGYILHGAPKLTCQSDGNWDAEIPLCKPVNCGPPENOV8a DLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIENOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i DLAHGFPNGFSFIHGGHIQYQCFPGYKLHGNSSRRCLSNGSWSGSSPSCLPCRCSTPVIENOV8a YGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNANOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i YGTVNGTDFDCGKAARIQCFKGFKLLGLSEITCEADGQWSSGFPHCEHTSCGSLPMIPNANOV8a FISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVANOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i FISETSSWKENVITYSCRSGYVIQGSSDLICTEKGVWSQPYPVCEPLSCGSPPSVANAVANOV8a TGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i TGEAHTYESEVKLRCLEGYTMDTDTDTFTCQKDGRWFPERISCSPKKCPLPENITHILVHNOV8a GDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i GDDFSVNRQVSVSCAEGYTFEGVNISVCQLDGTWEPPFSDESCSPVSCGKPESPEHGFVVNOV8a GSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i GSKYTFESTIIYQCEPGYELEGNRERVCQENRQWSGGVAICKETRCETPLEFLNGKADIENOV8a NRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKENOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i NRTTGPNVVYSCNRGYSLEGPSEAHCTENGTWSHPVPLCKPNPCPVPFVIPENALLSEKENOV8a FYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENATARGVHYQNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i FYVDQNVSIKCREGFLLQGHGIITCNPDETWTQTSAKCEKISCGPPAHVENAIARGVHYQNOV8a YGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i YGDMITYSCYSGYMLEGFLRSVCLENGTWTSPPICRAVCRFPCQNGGICQRPNACSCPEGNOV8a WMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHNOV8b ------------------------------------------------------------NOV8c ------------------------------------------------------------NOV8d ------------------------------------------------------------NOV8e ------------------------------------------------------------NOV8f ------------------------------------------------------------NOV8g ------------------------------------------------------------NOV8h ------------------------------------------------------------NOV8i WMGRLCEEPICILPCLNGGRCVAPYQCDCPPGWTGSRCHTAVCQSPCLNGGKCVRPNRCHNOV8a CLSSWTGHNCSRKRRTGFNOV8b ------------------NOV8c ------------------NOV8d ------------------NOV8e ------------------NOV8f ------------------NOV8g ------------------NOV8h ------------------NOV8i CLSSWTGHNCSRKRRTGFNOV8a (SEQ ID NO: 178)NOV8b (SEQ ID NO: 180)NOV8c (SEQ ID NO: 182)NOV8d (SEQ ID NO: 184)NOV8e (SEQ ID NO: 186)NOV8f (SEQ ID NO: 188)NOV8g (SEQ ID NO: 190)NOV8h (SEQ ID NO: 192)NOV8i (SEQ ID NO: 194)


Further analysis of the NOV8a protein yielded the following properties shown in Table 8C.

TABLE 8CProtein Sequence Properties NOV8aSignalP analysis:Cleavage site between residues 17 and 18PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 3; pos. chg 2; neg. chg 0H-region:length 23; peak value 8.53PSG score:4.12GvH:von Heijne's method for signal seq recognitionGvH score (threshold: −2.1): −2.66possible cleavage site: between 16 and 17>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 0PERIPHERALLikelihood = 0.58 (at 253)ALOM score: −0.16 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 6Charge difference: −2.0 C(1.0)-N(3.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content: 4Hyd Moment(75):11.27Hyd Moment(95):11.90G content:2D/E content: 1S/T content:5Score: 0.60Gavel:prediction of cleavage sites for mitochondrial preseqR-10 motif at 37 SRN FSNUCDISC: discrimination of nuclear localization signalspat4: KKRH (3) at 1183pat4: RKRR (5) at 3564pat7: nonebipartite: nonecontent of basic residues: 8.0%NLS Score: 0.03KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: RRICnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: foundRLGGSVPHL at 1632VAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):65.2%: nuclear21.7%: mitochondrial 8.7%: cytoplasmic 4.3%: vacuolar>> prediction for CG50646-04 is nuc (k = 23)


A search of the NOV8a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 8D.

TABLE 8DGeneseq Results for NOV8aNOV8aResidues/Identities/GeneseqProtein/Organism/LengthMatchSimilarities for theExpectIdentifier[Patent #, Date]ResiduesMatched RegionValueABJ10589Human novel protein NOV2a SEQ ID1 . . . 3570 3570/3570 (100%)0.0NO: 6 - Homo sapiens, 3570 aa.1 . . . 3570 3570/3570 (100%)[WO200259315-A2, 01-AUG-2002]ABJ10590Human novel protein NOV2b SEQ ID1 . . . 35703551/3570 (99%)0.0NO: 8 - Homo sapiens, 3568 aa.1 . . . 35683555/3570 (99%)[WO200259315-A2, 01-AUG-2002]AAE20146Human C3b/C4b complement3 . . . 35703502/3570 (98%)0.0receptor-like protein - Homo sapiens,4 . . . 35713519/3570 (98%)3571 aa. [WO200210388-A2, 07-FEB-2002]AAE20147Mouse C3b/C4b complement3 . . . 35692886/3570 (80%)0.0receptor-like protein - Mus musculus,31 . . . 3593 3174/3570 (88%)3594 aa. [WO200210388-A2, 07-FEB-2002]AAM93954Human polypeptide, SEQ ID NO:2074 . . . 3570  1485/1497 (99%)0.04153 - Homo sapiens, 1497 aa.1 . . . 14971487/1497 (99%)[EP1130094-A2, 05-SEP-2001]


In a BLAST search of public sequence databases, the NOV8a protein was found to have homology to the proteins shown in the BLASTP data in Table 8E.

TABLE 8EPublic BLASTP Results for NOV8aNOV8aProteinResidues/Identities/AccessionMatchSimilarities for theExpectNumberProtein/Organism/LengthResiduesMatched PortionValueQ9ES77Polydom protein precursor - Mus3 . . . 35692894/3570(81%)0.0musculus (Mouse), 3567 aa.4 . . . 35663180/3570(89%)Q8NBT9Hypothetical protein FLJ90754 -2074 . . . 3570  1485/1497(99%)0.0Homo sapiens (Human), 1497 aa.1 . . . 14871487/1497(99%)Q96JU7Hypothetical protein FLJ14964 -231 . . . 1546 1255/1316(95%)0.0Homo sapiens (Human), 1316 aa.1 . . . 13141267/1316(95%)Q8C720Polydomain protein - Mus3 . . . 845 694/846(82%)0.0musculus (Mouse), 848 aa4 . . . 846 748/846(88%)(fragment).Q922H0Polydomain protein - Mus2902 . . . 3569  534/668(79%)0.0musculus (Mouse), 669 aa2 . . . 668 594/668(87%)(fragment).


PFam analysis predicts that the NOV8a protein contains the domains shown in the Table 8F.

TABLE 8FDomain Analysis of NOV8aIdentities/SimilaritiesPfam DomainNOV8a Match Regionfor the Matched RegionExpect Valuevwa 80 . . . 25657/200(28%)5.1e−37128/200(64%)Metallothio_PEC269 . . . 32422/80(28%)0.1527/80(34%)sushi377 . . . 43218/64(28%)  1e−0639/64(61%)sushi437 . . . 49221/64(33%)4.6e−1448/64(75%)sushi497 . . . 55812/68(18%)0.1739/68(57%)HYR560 . . . 64129/86(34%)6.1e−1657/86(66%)HYR642 . . . 72127/86(31%)3.4e−1654/86(63%)TNFR_c61017 . . . 105514/48(29%)0.7434/48(71%)EGF1196 . . . 122718/47(38%)3.9e−0724/47(51%)EGF1234 . . . 126518/47(38%)1.2e−1027/47(57%)EGF1272 . . . 130317/47(36%)6.9e−1028/47(60%)EGF1348 . . . 137913/47(28%)1.3e−0826/47(55%)EGF1386 . . . 141717/47(36%)6.9e−1028/47(60%)pentaxin1430 . . . 162257/209(27%)  5e−1499/209(47%)sushi1630 . . . 168418/64(28%)2.3e−1042/64(66%)sushi1689 . . . 174225/63(40%)3.3e−1743/63(68%)EGF1748 . . . 178214/47(30%)1.8e−0526/47(55%)sushi1788 . . . 184124/63(38%)  2e−1543/63(68%)sushi1846 . . . 189920/63(32%)4.2e−1445/63(71%)sushi1904 . . . 195722/63(35%)8.5e−1442/63(67%)sushi1962 . . . 201522/63(35%)1.9e−1339/63(62%)sushi2020 . . . 207719/64(30%)5.4e−0742/64(66%)sushi2082 . . . 214017/65(26%)2.3e−0839/65(60%)sushi2145 . . . 219824/63(38%)3.8e−1846/63(73%)sushi2203 . . . 225819/64(30%)3.3e−1045/64(70%)sushi2263 . . . 231726/63(41%)9.4e−1746/63(73%)sushi2322 . . . 237521/63(33%)3.2e−0841/63(65%)sushi2380 . . . 243424/63(38%)1.9e−1343/63(68%)sushi2439 . . . 249221/63(33%)3.8e−1142/63(67%)sushi2497 . . . 255026/63(41%)3.4e−1543/63(68%)sushi2555 . . . 260722/63(35%)1.1e−1238/63(60%)sushi2659 . . . 271121/65(32%)2.9e−1043/65(66%)sushi2716 . . . 276919/63(30%)9.6e−1038/63(60%)sushi2774 . . . 282720/63(32%)5.2e−1445/63(71%)sushi2832 . . . 288522/63(35%)1.1e−1442/63(67%)sushi2890 . . . 294321/63(33%)7.6e−1235/63(56%)sushi2948 . . . 300119/63(30%)7.6e−1340/63(63%)sushi3006 . . . 305821/62(34%)3.8e−0837/62(60%)sushi3063 . . . 311622/63(35%)5.7e−1144/63(70%)sushi3121 . . . 317517/64(27%)1.1e−1040/64(62%)sushi3180 . . . 323516/62(26%)1.2e−0539/62(63%)sushi3240 . . . 329319/63(30%)1.2e−1244/63(70%)sushi3298 . . . 335123/63(37%)1.6e−1041/63(65%)sushi3356 . . . 341018/64(28%)3.6e−0941/64(64%)sushi3415 . . . 346722/63(35%)1.5e−1242/63(67%)EGF3467 . . . 349813/47(28%) 0.002922/47(47%)EGF3503 . . . 353018/47(38%)  2e−0524/47(51%)EGF3535 . . . 356213/47(28%) 0.003422/47(47%)


Example 9

The NOV9 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 9A.

TABLE 9ANOV9 Sequence AnalysisNOV9a, CG50736-09SEQ ID NO: 2073260 bpDNA SequenceORF Start: ATG at 171ORF Stop: TGA at 2862GGCACGAGCAGGAGCTTCCCAAGAACCCGAAAACTTCCCAGTATTTCTTCCAGTTGCAGGAGCATTTCGTGAAAGATCTGGTCGGCCCAGGCCCCTTCACTGTTTTTGCACCTTTATCTGCAGCCTTTGATGAGGAAGCTCGGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAATAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGATGTGAACTGCACCTGCAAGGTGGGCTATGTGGGAGATGGCTTCTCATGCAGTGGGAACCTGCTGCAGGTCCTGATGTCCTTCCCCTCACTCACAAACTTCCTGACGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAGCACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGGTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACCCACTCCAACCGACGGAGACCAGGTTTGTTGATGGAAGAGCCATTCTGCAGTGGGACATCTTTGCCTcCAATGGGATCATTCATGTCATTTCCAGGCCTTTAAAAGCACCCCCTGCCCCCGTGACCTTGACCCACACTGGCTTGGGAGCAGGGATCTTCTTTGCCATCATCCTGGTGACTGGGGCTGTTGCCTTGGCTGCTTACTCCTACTTTCGGATAAACCGGAGAACAATCGGCTTCCAGCATTTTGAGTCGGAAGAGGACATTAATGTTGCAGCTCTTGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAACCTTCCTACGACCCCTTCACGGACTCTGAAGAACGGCAGCTTGAGGGCAATGACCCCTTGAGGACACTGTGAGGGCCTGGACGGGAGATGCCAGCCATCACTCACTGCCACCTGGGCCATCAACTGTGAATTCTCAGCACCAGTTGCCTTTTAGGAACGTAAAGTCCTTTAAGCACTCAGAAGCCATACCTCATCTCTCTGGCTGATCTGGGGGTTGTTTCTGTGGGTGAGAGATGTGTTGCTGTGCCCACCCAGTACAGCTTCCTCCTCTGACCCTTTGGCTCTTCTTCCTTTGTACTCTTCAGCTGGCACCTGCTCCATTCTGCCCTACATGATGGGTAACTGTGATCTTTCTTCCCTGTTAGATTGTAAGCCTCCNTCTTTGTATCCCAGCCCCTAGCCCAGTGCCTGACACAGGAACTGTGCACAATAAGGTTTATGGACAGAAAAAAAAAAAAAAAAAAAAAANOV9a, CG50738-09SEQ ID NO: 208897 aaMW at 97584.6kDProtein SequenceMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRVGSKLLITASQDPLQPTETRFVDGRAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILVTGAVALAAYSYFRINRRTIGFQHFESEEDINvAALGKQQPENISNPLYESTTSAPPEPSYDPFTDSEERQLEGNDPLRTLNOV9b, 197408749SEQ ID NO: 2091668 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCATGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAIAAGTCACTATGTCGGAGATGGGCTGAACTGTGGGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9b, 197408749SEQ ID NO: 210556 aaMW at 60171.4kDProtein SequenceGSRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKPNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCGPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRPNOV9c, CG50736-01SEQ ID NO: 2112011 bpDNA SequenceORF Start: ATG at 877ORF Stop: TGA at 1822GGGTGTGATGGGCTTCTAGTTTCTCTAGCTGCATCACCCTTGAACCATCCAGAGTCCCAGTAAGCCACGGGCTTGAGCATGGAGGAGAATCCTCAGAGACAGAACCCCTGCCCACATGTCTGGGCCTTGCTCAAGCCAGCAAGGGGCTGAATCCCTGTGTTTCAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTCGAAGGCTGCCGGGAGCGATGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGTCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGCCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCCAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAACACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGCTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACNOV9c,CG50736-01SEQ ID NO: 212315 aaMW at 33739.5kDProtein SequenceMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCADLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRMKGSAGLFQQLSSRPCISRTPDNOV9d, CG50736-02SEQ ID NO: 2131804 bpDNA SequenceORF Start: ATG at 877ORF Stop: TGA at 1609GGGTGTGATGGGCTTCTAGTTTCTCTAGCTGCATCACCCTTGAACCATCCAGAGTCCCAGTAAGCCACGGGCTTGAGCATGGAGGAGAATCCTCAGAGACAQAACCCCTGCCCACATGTCTGGGCCTTGCTCAAGCCAGCAAGGGGCTGAATCCCTGTGTTTCAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGATGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGTCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGAGAGAAGAGAAATGAGTATGAAAGACCTGGGCACCTACAAGAAAGAGAGGACACTTTTGTTCACCCAGTGGCTCAATCAACCAGTCAACATCTAATGACCACCTACTGTGTGCCAGGCACAGAGGTGCAATAGGCAAAGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGCAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTNOV9d, CG50736-02SEQ ID NO: 214244 aaMW at 26106.9kDProtein SequenceMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVCTPPCSANATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKREEKNOV9e, CG50736-03SEQ ID NO: 2152512 bpDNA SequenceORF Start: ATG at 416ORF Stop: TAA at 2423ATAGGGCTCGAGCGGCTGCCCGGGCAGGTCTCATGCCTCAGCCTCCGGAGTAGTATTTTTAGTAGAGATGGTGTTTACCATGTGGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCCACCCGCCTCGGCCTCCCAGAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCCTTGTTTGTATTTTGAATTCCAAATGGAAATACCTTCATGATCTTCCCACTACTAAAGGTTTAAATCTGGCACTGATACCTCTCCAAGAGGGCTATATACTATGCAGTGTTTCCCAGCATGTTTCACAAGAAAATTCTTTTTTGAGGATCATCTCACAGAACTTGGGATCTTTGCAACATGTATTGTGAAATCCAGGCCAGAGGAACCCCATGTTCCTTCCACACTGATATTCCACAATGGAGGCAAGAAAGGAGCTAGAGTCACTTCCTCCCTTTTGTCTGAACAGCCTCCACTCTATAATCCTGACCACAAAGCTTACTTCCCAGAGTCTGGGTGGGCCGAGAGGTGTGGAAGAGAGAATGGAGGACAGGAGAGCCAAATGGCACATTGCAGCAAAAGACTCCTGCCTCTGGCTGAAACCCTCTGATCTTCTGTTACAGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGACGTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGCGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGTTGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGTCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGACCTGGTATTCCTTTACCAAGGAATAAAGCCTTTGATGCCAGGACCCAGACTCAAGGAGAATCTGAATCTCTGCTCTCCTGCTTGCTGGTCATGTGGCCTTGATATCAAGCCACNOV9e, CG50736-03SEQ ID NO: 216669 aaMW at 73167.6kDProtein SequenceMEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERMEDRRAKWHIAAKDSCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLTSNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPVAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKEACANEAATMATYNQLSYAQKTWYSFTKENOV9f, CG50736-04SEQ ID NO: 2172483 bpDNA SequenceORF Start: ATG at 387ORF Stop: TAA at 2394CTCATGCCTCAGCCTCCGGAGTAGTATTTTTAGTAGAGATGGTGTTTACCATGTGGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCCACCCGCCTCGGCCTCCCAGAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCCTTGTTTGTATTTTGAATTCCAAATGGGAATTCCTTCATGATCTTCCCACTACTAAAGGTTTAAATCTGGCACTGATACCTCTCCAAGAGGGCTATATACTATGCAGTGTTTCCCAGCATGTTTCACAAGAAAATTCTTTTTTGAGGATCATCTCACAGAACTTGGGATCTTTGCAACATGTATTGTGAAATCCAGGCCAGAGGAACCCCATGTTCCTTCCACACTGATATTCCACAATGGAGGCAAGAAAGGAGCTAGAGTCACTTCCTCCCTTTTGTCTGAACAGCCTCCACTCTATAATCCTGACCACAAAGCTTACTTCCCAGAGTCTGGGTGGGCCGAGAGGTGTGGAAGAGAGAATGGAGGACAGGAGAGCCAAATGGCACATTGCAGCAAAAGACTCCTGCCTCTGGCTGAAACCCTCTGATCTTCTGTTACAGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAAACCAAAGGGTGTGAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGATGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCdACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGTCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGACCTGGTATTCCTTTACCAAGGAATAAAGCCTTTGATGCCAGGACCCAGACTCAAGGAGAATCTGAATCTCTGCTCTCCTGCTTGCTGGTCATGTGGCCTTGATATCAAGCCACNOV9f, CG50736-04SEQ ID NO: 218669 aaMW at 73183.5kDProtein SequenceMEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERMEDRPAKWHIAAKDSCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKTWYSFTKENOV9g, CG50736-05SEQ ID NO: 2191353 bpDNA SequenceORF Start: at 1ORF Stop: TAA at 1264CTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGATGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGTCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGACCTGGTATTCCTTTACCAAGGAATAAAGCCTTTGATGCCAGGACCCAGACTCAAGGAGAATCTGAATCTCTGCTCTCCTGCTTGCTGGTCATGTGGCCTTGATATCAAGCCACNOV9g, CG50736-05SEQ ID NO: 220421 aaMW at 45276.6kDProtein SequenceLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKTWYSFTKENOV9h, CG50736-06SEQ ID NO: 2213625 bpDNA SequenceORF Start: ATG at 387ORF Stop: TAG at 2022CTCATGCCTCAGCCTCCGGAGTAGTATTTTTAGTAGAGATGGTGTTTACCATGTGGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCCACCCGCCTCGGCCTCCCAGACTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCCTTGTTTGTATTTTGAATTCCAAATGGAAATACCTTCATGATCTTCCCACTACTAAAGGTTTAAATCTGGCACTGATACCTCTCCAAGAGGGCTATATACTATGCAGTGTTTCCCAGCATGTTTCACAAGAAAATTCTTTTTTGAGGATCATCTCACAGAACTTGGGATCTTTGCAACATGTATTGTGAAATCCAGGCCAGAGGAACCCCATGTTCCTTCCACACTGATATTCCACAATGGAGGCAAGAAAGGAGCTAGAGTCACTTCCTCCCTTTTGTCTGAACAGCCTCCACTCTATAATCCTGACCACAAAGCTTACTTCCCAGAGTCTGGGTGGGCCGAGAGGTGTGGAAGAGAGAATGGAGGACAGGAGAGCCAAATGGCACATTGCAGCAAAAGACTCCTGCCTCTGGCTGAAACCCTCTGATCTTCTGTTACAGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAATAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGQAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGGTGAGGGTGCCTCTTCCCCCCTCGCAACTCTAAAAGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAGCACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGCTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACCCACTCCAACCGACGGAGACCAGGTTTGTTGATGGAAGAGCCATTCTGCAGTGGGACATCTTTGCCTCCAATGGGATCATTCATGTCATTTCCAGGCCTTTAAAAGCACCCCCTGCCCCCGTGACCTTGACCCACACTGGCTTGGGAGCAGGGATCTTCTTTGCCATCATCCTGGTGACTGGGGCTGTTGCCTTGGCTGCTTACTCCTACTTTCGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAATTGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAACCTTCCTACGACCCCTTCACGGACTCTGAAGAACGGCAGCTTGAGGGCAATGACCCCTTGAGGACACTGTGAGGGCCTGGACGGGAGATGCCAGCCATCACTCACTGCCACCTGGGCCATCAACTGTGAATTCTCAGCACCAGTTGCCTTTTAGGAACGTAAAGTCCTTTAAGCACTCAGAAGCCATACCTCATCTCTCTGGCTGATCTGGGGGTTGTTTCTGTGGGTGAGAGATGTGTTGCTGTGCCCACCCAGTACAGCTTCCTCCTCTGACCCTTTGGCTCTTCTTCCTTTGTACTCTTCAGCTGGCACCTGCTCCATTCTGCCCTACATGATGGGTAACTGTGATCTTTCTTCCCTGTTAGATTGTAAGCCTCCGTCTTTGTATCCCAGCCCCTAGCCCAGTGCCTGACACAGGAACTGTGCACAATAAAGGTTTATGGAACAGAAACAAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACNOV9h, CG50736-06SEQ ID NO: 222545 aaMW at 60038.8kDProtein SequenceMEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERMEDRRAKWHIAAKDSCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQGEGASSPLATLKVSALISTRPPESVNATPASMGRRVRCAGRGDLGLIVCPVAAQTTDSAMMASRAPGSASVKRGGQAPRVTLRQFCLQCVRLLVLLMPPVRRTTRVSVTWIMKVTESHAQLWISANRTTGAVQRWPDAPRRARRSPAAARRDTKGTGTAAQRNOV9i, CG50736-07SEQ ID NO: 2231624 bpDNA SequenceORF Start: ATG at 387ORF Stop: TAA at 1530CTCATGCCTCAGCCTCCGGAGTAGTATTTTTAGTAGAGATGGTGTTTACCATGTGGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCCACCCGCCTCGGCCTCCCAGAGTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCCTTGTTTGTATTTTGAATTCCAAATGGAAATACCTTCATGATCTTCCCACTACTAAAGGTTTAAATCTGGCACTGATACCTCTCCAAGAGGGCTATATACTATGCAGTGTTTCCCAGCATGTTTCACAAGAAAATTCTTTTTTGAGGATCATCTCACAGAACTTGGGATCTTTGCAACATGTATTGTGAAATCCAGGCCAGAGGAACCCCATGTTCCTTCCACACTGATATTCCACAATGGAGGCAAGAAAGGAGCTAGAGTCACTTCCTCCCTTTTGTCTGAACAGCCTCCACTCTATAATCCTGACCACAAAGCTTACTTCCCAGAGTCTGGGTGGGCCGAGAGGTGTGGAAGAGAGAATGGAGGACAGGAGAGCCAAATGGCACATTGCAGCAAAAGACTCCTGCCTCTGGCTGAAACCCTCTGATCTTCTGTTACAGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCNOV9i, CG50736-07SEQ ID NO: 224381 aaMW at 42565.8kDProtein SequenceMEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERMEDRRAKWHIAAKDSCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPSLPWRTRCPVNOV9j, CG50736-08SEQ ID NO: 2251857 bpDNA SequenceORF Start: at 1ORF Stop: TAA at 1768GTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGATGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGTCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGACCTGGTATTCCTTTACCAAGGAATAAAGCCTTTGATGCCAGGACCCAGACTCAAGGAGAATCTGAATCTCTGCTCTCCTGCTTGCTGGTCATGTGGCCTTGATATCAAGCCACNOV9j, CG50736-08SEQ ID NO: 226589 aaMW at 63994.0kDProtein SequenceVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKTWYSFTKENOV9k, CG50736-10SEQ ID NO: 2278495 bpDNA SequenceORF Start: ATG at 210ORF Stop: TGA at 7461AATCATCCCACATGCTAAGAATCTAAGATGTATAAAATAAAGTGGTGAAAGATGAAAATGAAATTTTATCAAGGTTAGAGTCAGGTTGGAGTGGCCATTGTTTACCAAACTGAGAAATCTAAATTTTATTTGGTTGGTAATTGAGAGTCATTGAGATATTTTGGGGAAGGTCACCCTGATGCCTTTGCTAATCAAATGAAATGAATGAAATGGAGAGGCAAGAAACTGGAAATAGCAAGACGAGGTATCATGCTACTGCAATAGTCCAGGCAAAACATGATAAAGGCCTCAACAAGAATGGCACCAGTGGAGATGAAGAGCAGAAGATCAAGGTGGGAGACAGAGACAGAGAAAACAAAGGATTTGATGGCTTATTAGATGTTTGGAATACTTTAAACTTTATTCATCCTTGCTTTGCTGTGTGCAACTGTGTGCATGGGGTGTGCAACAGTGGACTAGATGGCGATGGAACCTGTGAGTGCTACTCTGCGTACACTGGCCCCAAGTGTGACAAGCTCACAGAAAACTTTCACACCTCTCATCTGACACTGTGGCCTGTGCACGACTCCAAGCACTGGGGAAGCCTTCGACATCAGAATATGAATGGCACCTGTTCTTCCGGGGGCGGCAAGGGGGATCCCGATGTTTATCAAAATGGGTTGATTTTCCACGGAGGGGGTACTTCTGGAGGTCTATCGTCATCACGAAACAGACGAAGTAGTGTCAAGCGTCCTGAGAAGTGGAAGGGGGACGATCGAGATGGAGGTGGCAAGGAAGGCCAGCAGCGGCGGCGGGCAGACACAGAGTCGAGTCTTCAAAGAGGTCACATCAAAACGCCCCTGCCCCACAGGCAAGGTGAAGCGCGGATCACGGAGACAACGGGGAATTGTGTTTCTGCTGGCATGACTGGAACCAATGCCAATCACACAAAAGTTCACCCTACGGTTCAGTCCTTGACAGAATATGATTCCTTTCAGACTCATTCCACCAGCAGACTGAAGGAATTTGAGAAACAGCAGGTGAAGGAAAGATTTTCTGACCCTCCCCTAATGCAGGCTATAAAACCCTCACATGAGAAGTACCCTCCTTATGCCCAGAGAAAAGGAACATCTTTGTCTCCAAAGACACAGGGACACGGAGATGATGAACAGGCCTTGCTAAGTTTCCTCCACTCTATTACCCTTAGCTTGTACCTTTATCCAACCACATTCTTCCATGACTCTCCAGTCTTCATCAAACCTGGCATAAAAACACTCAGACTTAACCACTTCTTTGGGTCTTCATTTCCTTATGAAGGCTCCAGTGTCATANNNNNNATGGGAATTGAGGTTTGGAAAAACTGGTGCCAAAATGCTGATACCCTGGCTGCTGCCCCTGCTCCATCCCTGAATGTGCAGCCTTGCTCTGCCCAGAAAATTCCAGATGTTCGCCTTCCACTGAAGATGAAAACAAACTGGAATGCAAATGCCTTCCCAATTACCGAGGCGATGGCAAATACTGCGACCCCATCAATCCATGTTTACGAAAAATCTGCCACCCTCATGCTCATTGTACGTACCTGGGACCAAATCGGCACAGTTGTACATGCCAAGAAGGCTACCGTGGGGATGGCCAAGTGTGCTTGCCTGTGGACCCCTGCCAAATTAACTTTGGAAACTGCCCTACAAAGTCTACAGTGTGCAAATATGATGGGCCTGGACAGATGCATTTGCCAGAAAGGTTACGTGGGTGATGGCTTAACGTGTTATGGAAACATTATGGAGCGACTCAGAGAATTAAATACTGAACCCAGAGGAAAATGGCAAGGAAGGCTGACCTCTTTCATCTCACTCCTAGAAAGTATACAAATTGTAAGTGTACAACTCAGTGAATTTTCCCAACGTGAACCTACTTGTGTAAACACCAAGTCCATTGCCAGCAACCTAGAAGGCCCCCTGGTCCCCCTTTCCAATCATTACCCTCTACAGGTAAATGAGCTTTTGGTGGATAATAAAGCTGCTCAATACTTTGTGAAACTCCACATAATTGCTGGTCAGATGAACATCGAATATATGAATAACACAGACATGTTCTACACCTTGACTGGAAAGTCGGGGGAAATCTTCAACAGCGATAAGGACAATCAAATAAAGCTTAAACTCCATGGAGGCAAAAAGAAGGTAAAAATTATACAAGGGGACATCATTGCTTCCAATGGGCTTCTGCACATCCTTGACAGAGCCATGGACAAGTTAGAACCCACATTTGAGAGCAACAATGAGGALACCAATTTGGGACATGCCTTAGATGAGGATGGAGTTGGTGGACCATACACCATTTTTGTTCCAAATAATGAAGCATTGAATAACATGAAGGACGGCACTCTCGATTACCTCCTTTCTCCAGAGCTTGAAGTGGCCACTCTCATCTCCACCCCTCACATCAGGAGCATGGCCAACCAGCTCATACAGTTCAACACCACCGACAATGGACAGATTCTGGCAAATGATGTGGCAATGGAAGAAATTGAGATCACTGCCAAAAATGGCCGAATTTACACACTGACAGGAGTTCTCATTCCTCCCTCCATTGTCCCGATTCTGCCCCATCGATGTGATGAAACAAAGAGAGAGATGAAACTGGGCACTTGTGTGAGCTGTTCTCTGGTGTACTGGAGCAGATGTCCTGCTAACTCTGAGCCCACAGCACTCTTCACACACAGATGTGTCTACAGTGGCAGGTTTGGGAGCCTGAAGAGCGGCTGTGCCCGGTACTGCAATGCCACTGTGAAGTGTGCAGATAGCCTCGGCGGCAACGGGACATGCATTTGTGAGGAGGGCTTCCAAGGCTCCCAGTGTCAGTTCTGCTCTGATCCCAATAAATACGGACCTCGGTGTAACAAAAAATGCCTGTGCGTTCACGGAACATGCAATAACAGGATAGACAGCGATGGGGCCTGCCTCACTGGCACATGCAGAGACGGCTCTGCCGGGAGACTCTGTGATAAGCAGACCTCAGCCTGTGGGCCCTACGTGCAGTTCTGTCACATCCACGCCACCTGTGAATACAGCAATGGGACAGCCAGTTGTATTTGCAAAGCAGGATATGAAGGAGATGGAACTCTGTGTTCTGAGATGGACCCTTGCACAGGACTAACTCCAGGAGGCTGTAGCCGCAATGCAGAATGCATCAAAACTGGCACGGGCACCCACACCTGCGTGTGTCAGCAGGGTTGGACAGGGAATGGGAGAGACTGCTCGGAGATCAACAACTGCCTGCTGCCCAGTGCAGGCGGCTGCCACGACAACGCATCCTGTTTGTATGTGGGTCCCGGGCAGAATGAGTGTGAGTGCAAGAAAGGATTTCGAGGAAATGGGATTGACTGTGAACCAATAACTTCATGCTTGGAACAAACCGGGAAATGTCATCCATTGGCAAGCTGTCAATCTACTTCGTCTGGTGTCTGGAGCTGTGTTTGTCAAGAGGGCTATGAAGGAGATGGCTTTCTGTGCTATGGAAACGCAGCAGTGGAATTGTCATTTCTCTCCGAAGCAGCTATATTTAACCGATGGATAAATAATGCTTCTCTACAACCCACACTGTCAGCCACCTCAAACCTCACTGTCCTCGTGCCTTCCCAACAAGCTACTGAGGACATGGACCAGGATGAGAAAAGCTTCTGGTTGTCACAGAGCAATATTCCAGCCCTAATAAAGTACCATATGCTACTAGGCACATACAGAGTGGCAGATCTGCAGACCCTGTCTTCTTCTGACATGTTGGCAACATCTTTGCAGGGCAACTTCCTTCACTTGGCAAAGGTGGATGGGAATATCACAATTGAAGGGGCCTCCATTGTCGATGGGGACAACGCAGCCACAAATGGAGTGATACACATCATCAACAAGGTGCTGGTCCCACAAAGACGTCTAACTGGCTCCTTACCAAACCTGCTCATGCGGCTGGAACAGATGCCTGACTATTCCATCTTCCGGGGCTACATCATTCAATATAATCTGGCGAATGCAATTGAGGCTGCCGATGCCTACACAGTGTTTGCTCCAAACAACAATGCCATCGAGAATTACATCAGGGAGAAGAAAGTCTTGTCTCTAGAGGAGGACGTCCTCCGGTATCATGTGGTCCTGGAGGAGAAACTCCTGAAGAATGACCTGCACAATGGCATGCATCGTGAGACCATGCTGGGTTTCTCCTATTTCCTTAGCTTCTTTCTCCATAATGACCAGCTCTATGTAAATGAGGCTCCAATAAACTACACCAATGTAGCCACTGATAAGGGAGTGATCCATGGTTTGGGAAAAGTTCTGGAAATTCAGAAGAACAGATGTGATAATAATGACACTACTATTATACGAGGAAGATGTAGGACATGCTCCTCAGAGCTGACCTGCCCATTCGGAACTAAATCTCTAGGTAATGAGAAGAGGAGATGCATCTATACCTCCTATTTCATGGGAAGACGAACCCTGTTTATTGGGTGCCAGCCAAAATGTGTGAGAACCGTCATTACGAGAGAATGCTGTGCCGGCTTCTTTGGCCCCCAATGCCAGCCCTGCCCAGGGAATGCCCAGAATGTCTGCTTTGGTAATGGCATCTGTTTGGATGGAGTGAATGGCACAGGTGTGTGTGAGTGTGGGGAGGGCTTCAGCGGCACAGCCTGCGAGACCTGCACCGAGGGCAAGTACGGCATCCACTGTGACCAAGCATGTTCTTGTGTCCATGGGAGATGCAACCAAGGACCCTTGGGAGATGGCTCCTGTGACTGTGATGTTGGCTGGCGAGGAGTGCATTGTGACAATGCAACCACAGAAGACAACTGCAATGGGACATGCCATACCAGCGCCAACTGCCTCACCAACTCAGATGGTACAGCTTCATGCAAGTGTGCAGCAGGATTCCAAGGAAACGGGACCATCTGCACAGCAATCAATGCCTGTGAGATCAGCAATGGAGGTTGCTCTGCCAAGGCTGACTGTAAGAGAACCACCCCAGGAAGGCGAGTGTGCACGTGCAAAGCAGGCTACACGGGTGATGGCATTGTGTGCCTGGAAATCAACCCGTGTTTGGAGAACCATGGTGGCTGTGACAAGAATGCGGAGTGCACACAGACAGGACCCAACCAGGCTGCCTGTAACTGTTTGCCAGCATACACTGGAGATGGAAAGGTCTGCACACTCATCAATGTCTGCTTAACTAAAAATGGCGGCTGTAGTGAATTTGCCATCTGCAACCACACTGGGCAAGTAGAAAGGACTTGTACTTGCAAGCCAAACTACATTGGAGATGGATTTACCTGCCGCGGCAGCATTTATCAGGAGCTTCCCAAGAACCCGAAAACTTCCCAGTATTTCTTCCAGTTGCAGGAGCATTTCGTGAAAGATCTGGTCGGCCCAGGCCCCTTCACTGTTTTTGCACCTTTATCTGCAGCCTTTGATGAGGAAGCTCGGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTACAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAGCACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGCTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACCCACTCCAACCGACGGAGACCAGGTTTGTTGATCGAAGAGCCATTCTGCAGTGGGACATCTTTGCCTCCAATGGGATCATTCATGTCATTTCCAGGCCTTTAAAAGCACCCCCTGCCCCCGTGACCTTGACCCACACTGGCTTGGGAGCAGGGATCTTCTTTGCCATCATCCTGGTGACTGGGGCTGTTGCCTTGGCTGCTTACTCCTACTTTCGGATAAACCGGAGAACAATCGGCTTCCAGCATTTTGAGTCGGAAGAGGACATTAATGTTGCAGCTCTTGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAACCTTCCTACGACCCCTTCACGGACTCTGAAGAACGGCAGCTTGAGGGCAATGACCCCTTGAGGACACTGTGAGGGCCTGGACGGGAGATGCCACCCATCACTCACTGCCACCTGGGCCATCAACTGTGAATTCTCAGCACCAGTTGCCTTTTAGGAACGTAAAGTCCTTTAAGCACTCAGAAGCCATACCTCATCTCTCTGGCTGATCTGGGGGTTGTTTCTGTGGGTGAGAGATGTGTTGCTGTGCCCACCCAGTACAGCTTCCTCCTCTGACCCTTTGGCTCTTCTTCCTTTGTACTCTTCAGCTGGCACCTGCTCCATTCTGCCCTACATGATGGGTAACTGTGATCTTTCTTCCCTGTTAGATTGTAAGCCTCCGTCTTTGTATCCCAGCCCCTAGCCCAGTGCCTGACACAGGAACTGTGCACAATAAAGGTTTATGGAACAGAAACAAAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACNOV9k, CG50736-10SEQ ID NO: 2282417 aaMW at 262971.8kDProtein SequenceMERQETGNSKTRYHATAIVQAKHDKGLNKNGTSGDEEQKIKVGDRDRENKGFDGLLDVWNTLNFIHPCFAVCNCVHGVCNSGLDGDGTCECYSAYTGPKCDKLTENFHTSHLTLWPVHDSKHWGSLRHQNMNGTCSSGGGKGDPDVYQNGLIFHGGGTSGGLSSSRNRRSSVKRPEKWKGDDRDGGGKEGQQRRRADTESSLQRGHIKTPLPHRQGEARITETTGNCVSAGMTGTNANHTKVHPTVQSLTEYDSFQTHSTSRLKEFEKQQVKERFSDPPLMQAIKPSHEKYPPYAQRKGTSLSPKTQGHGDDEQALLSFLHSITLSLYLYPTTFFHDSPVFIKPGIKTLRLNHFFGSSFPYEGSSVIXXMGIEVWKNWCQNADTLAAAPAPSLNVQPCSAQKIPDVRLPLKMKTNWNANAFPITEAMANTATPSIHVYEKSATLMLIVRTWDQIGTVVHAKKATVGMAKCACLWTPAKLTLETALQSLQCANNMGLDRCICQKGYVGDGLTCYGNIMERLRELNTEPRGKWQGRLTSFISLLESIQIVSVQLSEFSQREPTCVNTKSIASNLEGPLVPLSNHYPLQVNELLVDNKAAQYFVKLHIIAGQNNIEYMNNTDMFYTLTGKSGEIFNSDKDNQIKLKLHGGKKKVKIIQGDIIASNGLLHILDRAMDKLEPTFESNNEETNLGHALDEDGVGGPYTIFVPNNEALNNMKDGTLDYLLSPELEVATLISTPHIRSMANQLIQFNTTDNGQILANDVAMEEIEITAKNGRIYTLTGVLIPPSIVPILPHRCDETKREMKLGTCVSCSLVYWSRCPANSEPTALFTHRCVYSGRFGSLKSGCARYCNATVKCADSLGGNGTCICEEGFQGSQCQFCSDPNKYGPRCNKKCLCVHGTCNNRIDSDGACLTGTCRDGSAGRLCDKQTSACGPYVQFCHIHATCEYSNGTASCICKAGYEGDGTLCSEMDPCTGLTPGGCSRNAECIKTGTGTHTCVCQQGWTGNGRDCSEINNCLLPSAGGCHDNASCLYVGPGQNECECKKGFRGNGIDCEPITSCLEQTGKCHPLASCQSTSSGVWSCVCQEGYEGDGFLCYGNAAVELSFLSEAAIFNRWINNASLQPTLSATSNLTVLVPSQQATEDMDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYSIFRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNNDTTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGIHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTASCKCAAGFQGNGTICTAINACEISNGGCSAKADCKRTTPGRRVCTCKAGYTGDGIVCLEINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCSEFAICNHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKNPKTSQYFFQLQEHFVKDLVGPGPFTVFAPLSAAFDEEARVKLWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTYRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRMKGSAGLFQQLSSRPCISRTPDNOV9l, CG50736-11SEQ ID NO: 2291599 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATNOV9l, CG50736-11SEQ ID NO: 230533 aaMW at 57827.8kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9m, CG50736-12JSEQ ID NO: 2311611 bpDNA SequenceORF Start: at 7ORF Stop: at 1606GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCGGNOV9m, CG50736-12SEQ ID NO: 232533 aaMW at 57827.8kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9n, CG50736-13SEQ ID NO: 2331668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCAATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCTTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAGAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9n, CG50736-13SEQ ID NO: 234552 aaMW at 59873.1kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCNDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKNTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDRAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9o, CG50736-14SEQ ID NO: 2351668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTCACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCTGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9o, CG50736-14SEQ ID NO: 236552 aaMW at 59831.1kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDLYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9p, CG50736-15SEQ ID NO: 2371668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGTCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGCGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9p, CG50736-15SEQ ID NO: 238552 aaMW at 59846.1kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9q, CG50736-16SEQ ID NO: 2391668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTTACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCCGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9q, CG50736-16SEQ ID NO: 240552 aaMW at 59948.2kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECYLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATRKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9r, CG50736-17SEQ ID NO: 2411668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGA~CAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAACTGCCAGAAGGGGTACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9r, CG50736-17SEQ ID NO: 242552 aaMW at 59873.1kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCNCQKGYKGDGHSCTETDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9s, CG50736-18SEQ ID NO: 2431668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAGGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTAGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9s, CG50736-18SEQ ID NO: 244552 aaMW at 59904.1kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQRCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKNTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSSVVGIVDYGPRPNKSEMWDVFCYNOV9t, CG50736-19SEQ ID NO: 2451668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTGCGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGCGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9t, CG50736-19SEQ ID NO: 246552 aaMW at 59786.0kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVACGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9u, CG50736-20SEQ ID NO: 2471668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCGTCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCACGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9u, CG50736-20SEQ ID NO: 248552 aaMW at 59802.0kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVVTDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATTATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9v, CG50736-21SEQ ID NO: 2491668 bpDNA SequenceORF Start: at 7ORF Stop: at 1663GGATCCAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTTCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTCGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGCCGNOV9V, CG50736-21SEQ ID NO: 250552 aaMW at 59846.1kDProtein SequenceRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9w, CG50736-22SEQ ID NO: 2514527 bpDNA SequenceORF Start: ATG at 4ORF Stop: TGA at 4510GACATGGACCAGGATGAGAAAAGCTTCTGGTTGTCACAGAGCAATATTCCAGCCCTAATAAAGTACCATATGCTACTAGGCACATACAGAGTGGCAGATCTGCAGACCCTGTCTTCTTCTGACATGTTGGCAACATCTTTGCAGGGCAACTTCCTTCACTTGGCAAAGGTGGATGGGAATATCACAATTGAAGGGGCCTCCATTGTCGATGGGGACAACGCAGCCACAAATGGAGTGATACACATCATCAACAAGGTGCTGGTCCCACAAAGACGTCTAACTGGCTCCTTACCAAACCTGCTCATGCGGCTGGAACAGATGCCTGACTATTCCATCTTCCGGGGCTACATCATTCAATATAATCTGGCGAATGCAATTGAGGCTGCCGATGCCTACACAGTGTTTGCTCCAAACAACAATGCCATCGAGAATTACATCAGGGAGAAGAAAGTCTTGTCTCTAGAGGAGGACGTCCTCCGGTATCATGTGGTCCTGGAGGAGAAACTCCTGAAGAATGACCTGCACAATGGCATGCATCGTGAGACCATGCTGGGTTTCTCCTATTTCCTTAGCTTCTTTCTCCACAATGACCAGCTCTATGTAAATGAGGCTCCAATAAACTACACCAATGTAGCCACTGATAAGGGAGTGATCCATGGCTTGGGAAAAGTTCTGGAAATTCAGAAGAACAGATGTGATAATAATGACACTACTATTATACGAGGAAGATGTAGGACATGCTCCTCAGAGCTGACCTGCCCATTCGGAACTAAATCTCTAGGTAATGAGAAGAGGAGATGCATCTATACCTCCTATTTCATGGGAAGACGAACCCTGTTTATTGGGTGCCAGCCAAAATGTGTGAGAACCGTCATTACGAGAGAATGCTGTGCCGGCTTCTTTGGCCCCCAATGCCAGCCCTGCCCAGGGAATGCCCAGAATGTCTGCTTTGGTAATGGCATCTGTTTGGATGGAGTGAATGGCACAGGTGTGTGTGAGTGTGGGGAGGGCTTCAGCGGCACAGCCTGCGAGACCTGCACCGAGGGCAAGTACGGCATCCACTGTGACCAAGCATGTTCTTGTGTCCATGGGAGATGCAACCAAGGACCCTTGGGAGATGGCTCCTGTGACTGTGATGTTGGCTGGCGAGGAGTGCATTGTGACAATGCAACCACAGAAGACAACTGCAATGGGACATGCCATACCAGCGCCAACTGCCTCACCAACTCAGATGGTACAGCTTCATGCAAGTGTGCAGCAGGATTCCAAGGAAACGGGACCATCTGCACAGCAATCAATGCCTGTGAGATCAGCAATGGAGGTTGCTCTGCCAAGGCTGACTGTAAGAGAACCACCCCAGGAAGGCGAGTGTGCACGTGCAAAGCAGGCTACACGGGTGATGGCATTGTGTGCCTGGAAATCAACCCGTGTTTGGAGAACCATGGTGGCTGTGACAAGAATGCGGAGTGCACACAGACAGGACCCAACCAGGCTGCCTGTAACTGTTTGCCAGCATACACTGGAGATGGAAAGGTCTGCACACTCATCAATGTCTGCTTAACTAAAAATGGCGGCTGTGGTGAATTTGCCATCTGCAACCACACTGGGCAAGTAGAAAGGACTTGTACTTGCAAGCCAAACTACATTGGAGATGGATTTACCTGCCGCGGCAGCATTTATCAGGAGCTTCCCAAGAACCCGAAAACTTCCCAGTATTTCTTCCAGTTGCAGGAGCATTTCGTGAAAGATCTGGTCGGCCCAGGCCCCTTCACTGTTTTTGCACCTTTATCTGCAGCCTTTGATGAGGAAGCTCGGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAAGACACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGCCTCGCAACTCTAAAAGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGATGTGAACTGCACCTGCAAGGTGGGCTATGTGGGAGATGGCTTCTCATGCAGTGGGAACCTGCTGCAGGTCCTGATGTCCTTCCCCTCACTCACAAACTTCCTGACGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAGCACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGGTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACCCACTCCAACCGACGGAGACCAGGTTTGTTGATGGAAGAGCCATTCTGCAGTGGGACATCTTTGCCTCCAATGGGATCATTCATGTCATTTCCAGGCCTTTAAAAGCACCCCCTGCCCCCGTGACCTTGACCCACACTGGCTTGGGAGCAGGGATCTTCTTTGCCATCATCCTGGTGACTGGGGCTGTTGCCTTGGCTGCTTACTCCTACTTTCGGATAAACCGGAGAACAATCGGCTTCCAGCATTTTGAGTCGGAAGAGGACATTAATGTTGCAGCTCTTGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAACCTTCCTACGACCCCTTCACGGACTCTGAAGAACGGCAGCTTGAGGGCAATGACCCCTTGAGGACACTGTGAGGGCCTGGACGGGAGNOV9w, CG50736-22SEQ ID NO: 2521502 aaMW at 163589.6kDProtein SequenceMDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYSIFRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNNDTTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGIHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTASCKCAAGFQGNGTICTAINACEISNGGCSAKADCKRTTPGRRVCTCKAGYTGDGIVCLEINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCGEFAICNHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKNPKTSQYFFQLQEHFVKDLVGPGPFTVFAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAXVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQPRNSKSVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRVGSKLLITASQDPLQPTETRFVDGPAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILVTGAVALAAYSYFRINRRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSYDPFTDSEERQLEGNDPLRTLNOV9x, CG50736-23SEQ ID NO: 2535416 bpDNA SequenceORF Stan: ATG at 478ORF Stop: TGA at 5008GTAGAATGCATCAAAACTGGCACGGGCACCCACACCTGCGTGTGTCAGCAGGGTTGGACAGGGAATGGGAGAGACTGCTCGGAGATCAACAACTGCCTGCTGCCCAGTGCAGGCGGCTGCCACGACAACGCATCCTGTTTGTATGTGGGTCCCGGGCAGAATGAGTGTGAGTGCAAGAAAGGATTTCGAGGAAATGGGATTGACTGTGCACCAATAACTTCATGCTTGGAACAAACCGGGAAATGTCATCCATTGGCAAGCTGTCAATCTACTTCGTCTGGTGTCTGGAGCTGTGTTTGTCAAGAGGGCTATGAAGGAGATGGCTTTCTGTGCTATGGAAACGCAGCAGTGGAATTGTCATTTCTCTCCGAAGCAGCTATATTTAACCGATGGATAAATAATGCTTCTCTACAACCCACACTGTCAGCCACCTCAAACCTCACTGTCCTCGTGCCTTCCCAACAAGCTACTGAGGACATGGACCAGGATGAGAAAAGCTTCTGGTTGTCACAGAGCAATATTCCAGCCCTAATAAAGTACCATATGCTACTAGGCACATACAGAGTGGCAGATCTGCAGACCCTGTCTTCTTCTGACATGTTGGCAACATCTTTGCAGGGCAACTTCCTTCACTTGGCAAAGGTGGATGGGAATATCACAATTGAAGGGGCCTCCATTGTCGATGGGGACAACGCAGCCACAAATGGAGTGATACACATCATCAACAAGGTGCTGGTCCCACAAAGACGTCTAACTGGCTCCTTACCAAACCTGCTCATGCGGCTGGAACAGATGCCTGACTATTCCATCTTCCGGGGCTACATCATTCAATATAATCTGGCGAATGCAATTGAGGCTGCCGATGCCTACACAGTGTTTGCTCCAAACAACAATGCCATCGAGAATTACATCAGGGAGAAGAAAGTCTTGTCTCTAGAGGAGGACGTCCTCCGGTATCATGTGGTCCTGGAGGAGAAACTCCTGAAGAATGACCTGCACAATGGCATGCATCGTGAGACCATGCTGGGTTTCTCCTATTTCCTTAGCTTCTTTCTCCACAATGACCAGCTCTATGTAAATGAGGCTCCAATAAACTACACCAATGTAGCCACTGATAAGGGAGTGATCCATGGCTTGGGAAAAGTTCTGGAAATTCAGAAGAACAGATGTGATAATAATGACACTACTATTATACGAGGAAGATGTAGGACATGCTCCTCAGAGCTGACCTGCCCATTCGGAACTAAATCTCTAGGTAATGAGAAGAGGAGATGCATCTATACCTCCTATTTCATGGGAAGACGAACCCTGTTTATTGGGTGCCAGCCAAAATGTGTGAGAACCGTCATTACGAGAGAATGCTGTGCCGGCTTCTTTGGCCCCCAATGCCAGCCCTGCCCAGGGAATGCCCAGAATGTCTGCTTTGGTAATGGCATCTGTTTGGATGGAGTGAATGGCACAGGTGTGTGTGAGTGTGGGGAGGGCTTCAGCGGCACAGCCTGCGAGACCTGCACCGAGGGCAAGTACGGCATCCACTGTGACCAAGCATGTTCTTGTGTCCATGGGAGATGCAACCAAGGACCCTTGGGAGATGGCTCCTGTGACTGTGATGTTGGCTGGCGAGGAGTGCATTGTGACAATGCAACCACAGAAGACAACTGCAATGGGACATGCCATACCAGCGCCAACTGCCTCACCAACTCAGATGGTACAGCTTCATGCAAGTGTGCAGCAGGATTCCAAGGAAACGGGACCATCTGCACAGCAATCAATGCCTGTGAGATCAGCAATGGAGGTTGCTCTGCCAAGGCTGACTGTAAGAGAACCACCCCAGGAAGGCGAGTGTGCACGTGCAAAGCAGGCTACACGGGTGATGGCATTGTGTGCCTGGAAATCAACCCGTGTTTGGAGAACCATGGTGGCTGTGACAAGAATGCGGAGTGCACACAGACAGGACCCAACCAGGCTGCCTGTAACTGTTTGCCAGCATACACTGGAGATGGAAAGGTCTGCACACTCATCAATGTCTGCTTAACTAAAAATGGCGGCTGTAGTGAATTTGCCATCTGCAACCACACTGGGCAAGTAGAAAGGACTTGTACTTGCAAGCCAAACTACATTGGAGATGGATTTACCTGCCGCGGCAGCATTTATCAGGAGCTTCCCAAGAACCCGAAAACTTCCCAGTATTTCTTCCAGTTGCAGGAGCACTTCGTGAAAGATCTGGTCGGCCCAGGCCCCTTCACTGTTTTTGCACCTTTATCTGCAGCCTTTGATGAGGAAGCTCGGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAACAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGATGTGAACTGCACCTGCAAGGTGGGCTATGTGGGAGATGGCTTCTCATGCAGTGGGAACCTGCTGCAGGTCCTGATGTCCTTCCCCTCACTCACAAACTTCCTGACGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAGCACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGCTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACCCACTCCAACCGACGGAGACCAGGTTTGTTGATGGAAGAGCCATTCTGCAGTGGGACATCTTTGCCTCCAATGGGATCATTCATGTCATTTCCAGGCCTTTAAAAGCACCCCCTGCCCCCGTGACCTTGACCCACACTGGCTTGGGAGCAGGGATCTTCTTTGCCATCATCCTGGTGACTGGGGCTGTTGCCTTGGCTGCTTACTCCTACTTTCGGATAAACCGGAGAACAATCGGCTTCCAGCATTTTGAGTCGGAAGAGGACATTAATGTTGCAGCTCTTGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAACCTTCCTACGACCCCTTCACGGACTCTGAAGAACGGCAGCTTGAGGGCAATGACCCCTTGAGGACACTGTGAGGGCCTGGACGGGAGATGCCACCCATCACTCACTGCCACCTGGGCCATCAACTGTGAATTCTCAGCACCAGTTGCCTTTTAGGAACGTAPAGTCCTTTAAGCACTCAGAAGCCATACCTCATCTCTCTGGCTGATCTGGGGGTTGTTTCTGTGGGTGAGAGATGTGTTGCTGTGCCCACCCAGTACAGCTTCCTCCTCTGACCCTTTGGCTCTTCTTCCTTTGTACTCTTCAGCTGGCACCTGCTCCATTCTGCCCTACATGATGGGTAACTGTGATCTTTCTTCCCTGTTTGTTAGATTGTAAGCCTCCGTCTTTGTATCCCAGCCCCTAGCCCAGTGCCTGACACAGGAACTGTGCACAATAAAGGTTTATGGAACAGAAACAAAGTCAACAGAAAAAAAAAAANOV9x, CG50736-23SEQ ID NO: 2541510 aaMW at 164274.3kDProtein SequenceMDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYSIFRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNNDTTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGIHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTASCKCAAGFQGNGTICTAINACEISNGGCSAKADCKRTTPGRRVCTCKAGYTGDGIVCLEINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCSEFAICNHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKNPKTSQYFFQLQEHFVKDLVGPGPFTVFAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRLGSKLLITASQDPLQPTETRFVDGRAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILVTGAVALAAYSYFRINPRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSYDPFTDSEERQLEGNDPLRTLNOV9y, CG50736-24SEQ ID NO: 2557670 bpDNA SequenceORF Start: ATG at 8ORF Stop: TGA at 7661TTTCCTCATGATGCTACAACATTTAGTAATTTTTTGTCTTGGATTGGTTGTACAAAATTTCTGCTCCCCAGCTGAAACCACAGGGCAGGCAAGAAGATGTGATAGGAAGTCTCTTCTTACAATTAGGACCGAGTGCCGATCCTGCGCTCTCAACCTTGGAGTCAAGTGCCCGGATGGTTACACCATGATTACCAGTGGCTCTGTAGGGGTTCGAGATTGCAGGTACACCTTTGAGGTCAGAACATACTCTCTGTCTCTCCCCGGATGCCGCCATATTTGTAGGAAGGACTATCTCCAACCTCGGTGTTGTCCTGGCCGCTGGGGCCCAGACTGTATAGAGTGCCCAGGTGGAGCGGGGTCACCCTGCAATGGCAGAGGCAGTTGTGCTGAAGGCATGGAAGGAAATGGAACCTGCTCCTGCCAAGAAGGGTTTGGTGGAACAGCCTGTGAAACCTGTGCTGACGACAACTTATTTGGACCCAGCTGTTCATCAGTGTGCAACTGTGTGCATGGGGTGTGCAACAGTGGACTAGATGGCGATGGAACCTGTGAGTGCTACTCTGCGTACACTGGCCCCAAGTGTGACAAGCCCATCCCTGAATGTGCAGCCTTGCTCTGCCCAGAAAATTCCAGATGTTCGCCTTCCACTGAAGATGAAAACAAACTGGAATGCAAATGCCTTCCCAATTACCGAGGCGATGGCAAATACTGCGACCCCATCAATCCATGTTTACGAAAAATCTGCCACCCTCATGCTCATTGTACGTACCTGGGACCAAATCGGCACAGTTGTACATGCCAAGAAGGCTACCGTGGGGATGGCCAAGTGTGCTTGCCTGTGGACCCCTGCCAAATTAACTTTGGAAACTGCCCTACAAAGTCTACAGTGTGCAAATATGATGGGCCTGGACAGTCTCACTGCGAGTGTAAGGAGCATTACCAGAATTTCGTACCTGGAGTGGGGTGCAGTATGACTGATATATGTAAATCAGATAACCCGTGTCATAGGAATGCAAATTGCACCACCGTCGCACCAGGCCGAACTGAATGCATTTGCCAGAAAGGTTACGTGGGTGATGGCTTAACGTGTTATGGAAACATTATGGAGCGACTCAGAGAATTAAATACTGAACCCAGAGGAAAATGGCAAGGAAGGCTGACCTCTTTCATCTCACTCCTAGACAAAGCTTATGCCTGGCCACTGAGTAAGCTGGGACCCTTCACGGTGCTGTTACCTACAGACAAGGGACTGAAAGGATTCAATGTAAATGAGCTTTTGGTGGATAATAAAGCTGCTCAATACTTTGTGAAACTCCACATAATTGCTGGTCAGATGAACATCGAATATATGAATAACACAGACATGTTCTACACCTTGACTGGAAAGTCGGGGGAAATCTTCAACAGCGATAAGGACAATCAAATAAAGCTTAAACTCCATGGAGGCAAAAAGAAGGTAAAAATTATACAAGGGGACATCATTGCTTCCAATGGGCTTCTGCACATCCTTGACAGAGCCATGGACAAGTTAGAACACACATTTGAGAGCAACAATGAGCAAACCATAATGACAATGCTACAACCAAGGTACAGCAAGTTCAGATCTTTGTTAGAGGAAACCAATTTGGGACATGCCTTAGATGAGGATGGAGTTGGTGGACCATACACCATTTTTGTTCCAAATAATGAAGCATTGAATAACATGAAGGACGGCACTCTCGATTACCTCCTTTCTCCAGAGGGATCTCGGAAGCTTCTGGAACTCGTCAGATACCACATTGTCCCATTTACCCAGCTTGAAGTGGCCACTCTCATCTCCACCCCTCACATCAGGAGCATGGCCAACCAGCTCATACAGTTCAACACCACCGACAATGGACAGATTCTGGCAAATGATGTGGCAATGGAAGAAATTGAGATCACTGCCAAAAATGGCCGAATTTACACACTGACAGGAGTTCTCATTCCTCCCTCCATTGTCCCGATTCTGCCCCATCGATGTGATGAAACAAAGAGAGAGATGAAACTGGGCACTTGTGTGAGCTGTTCTCTGGTGTACTGGAGCAGATGTCCTGCTAACTCTGAGCCCACAGCACTCTTCACACACAGATGTGTCTACAGTGGCAGGTTTGGGAGCCTGAAGAGCGGCTGTGCCCGGTACTGCAATGCCACTGTGAAGATTCCAAAGTGCTGCAAAGGCTTCTATGGACCTGACTGCAACCAGTGTCCAGGAGGCTTCTCAAATCCATGCTCAGGAAATGGACAGTGTGCAGATAGCCTCGGCGGCAACGGGACATGCATTTGTGAGGAGGGCTTCCAAGGCTCCCAGTGTCAGTTCTGCTCTGATCCCAATAAATACGGACCTCGGTGTAACAAAAAATGCCTGTGCGTTCACGGAACATGCAATAACAGGATAGACAGCGATGGGGCCTGCCTCACTGGCACATGCAGAGACGGCTCTGCCGGGAGACTCTGTGATAAGCAGACCTCAGCCTGTGGGCCCTATGTGCAGTTCTGTCACATCCACGCCACCTGTGAATACAGCAATGGGACAGCCAGTTGTATTTGCAAAGCAGGATATGAAGGAGATGGAACTCTGTGTTCTGAGATGGACCCTTGCACAGGACTAACTCCAGGAGGCTGTAGCCGCAATGCAGAATGCATCAAAACTGGCACGGGCACCCACACCTGCGTGTGTCAGCAGGGTTGGACAGGGAATGGGAGAGACTGCTCGGAGATCAACAACTGCCTGCTGCCCAGTGCAGGCGGCTGCCACGACAACGCATCCTGTTTGTATGTGGGTCCCGGGCAGAATGAGTGTGAGTGCAAGAAAGGATTTCGAGGAAATGGGATTGACTGTGAACCAATAACTTCATGCTTGGAACAAACCGGGAAATGTCATCCATTGGCAAGCTGTCAATCTACTTCGTCTGGTGTCTGGAGCTGTGTTTGTCAAGAGGGCTATGAAGGAGATGGCTTTCTGTGCTATGGAAACGCAGCAGTGGAATTGTCATTTCTCTCCGAAGCAGCTATATTTAACCGATGGATAAATAATGCTTCTCTACAACCCACACTGTCAGCCACCTCAAACCTCACTGTCCTCGTGCCTTCCCAACAAGCTACTGAGGACATGGACCAGGATGAGAAAAGCTTCTGGTTGTCACAGAGCAATATTCCAGCCCTAATAAAGTACCATATGCTACTAGGCACATACAGAGTGGCAGATCTGCAGACCCTGTCTTCTTCTGACATGTTGGCAACATCTTTGCAGGGCAACTTCCTTCACTTGGCAAAGGTGGATGGGAATATCACAATTGAAGGGGCCTCCATTGTCGATGGGGACAATGCAGCCACAAATGGAGTGATACACATCATCAACAAGGTGCTGGTCCCACAAAGACGTCTAACTGGCTCCTTACCAAACCTGCTCATGCGGCTGGAACAGATGCCTGACTATCCCATCTTCCGGGGCTACATCATTCAATATAATCTGGCGAATGCAATTGAGGCTGCCGATGCCTACACAGTGTTTGCTCCAAACAACAATGCCATCGAGAATTACATCAGGGAGAAGAAAGTCTTGTCTCTAGAGGAGGACGTCCTCCGGTATCATGTGGTCCTGGAGGAGAAACTCCTGAAGAATGACCTGCACAATGGCATGCATCGTGAGACCATGCTGGGTTTCTCCTATTTCCTTAGCTTCTTTCTCCATAATGACCAGCTCTATGTAAATGAGGCTCCAATAAACTACACCAATGTAGCCACTGATAAGGGAGTGATCCATGGCTTGGGAAAAGTTCTGGAAATTCAGAAGAACAGATGTGATAATAATGCCACTACTATTATACGAGGAAGATGTAGGACATGCTCCTCAGAGCTGACCTGCCCATTCGGAACTAAATCTCTAGGTAATGAGAAGAGGAGATGCATCTATACCTCCTATTTCATGGGAAGACGAACCCTGTTTATTGGGTGCCAGCCAAAATGTGTGAGAACCGTCATTACGAGAGAATGCTGTGCCGGCTTCTTTGGCCCCCAATGCCAGCCCTGTCCAGGGAATGCCCAGAATGTCTGCTTTGGTAATGGCATCTGTTTGGATGGAGTGAATGGCACAGGTGTGTGTGAGTGTGGGGAGGGCTTCAGCGGCACAGCCTGCGAGACCTGCACCGAGGGCAAGTACGGCATCCACTGTGACCAAGCATGTTCTTGTGTCCATGGGAGATGCAACCAAGGACCCTTGGGAGATGGCTCCTGTGACTGTGATGTTGGCTGGCGAGGAGTGCATTGTGACAATGCAACCACAGAAGACAACTGCAATGGGACATGCCATACCAGCGCCAACTGCCTCACCAACTCAGATGGTACAGCTTCATGCAAGTGTGCAGCAGGATTCCAAGGAAACGGGACCATCTGCACAGCAATCAATGCCTGTGAGATCAGCAATGGAGGTTGCTCTGCCAAGGCTGACTGTAAGAGAACCACCCCAGGAAGGCGAGTGTGCACGTGCAAAGCAGGCTACACGGGTGATGGCATTGTGTGCCTGGAAATCAACCCGTGTTTGGAGAACCATGGTGGCTGTGACAAGAATGCGGAGTGCACACAGACAGGACCCAACCAGGCTGCCTGTAACTGTTTGCCAGCATACACTGGAGATGGAAAGGTCTGCACACTCATCAATGTCTGCTTAACTAAAAATGGCGGCTGTAGTGAATTTGCCATCTGCAACCACACTGGGCAAGTAGAAAGGACTTGTACTTGCAAGCCAAACTACATTGGAGATGGATTTACCTGCCGCGGCAGCATTTATCAGGAGCTTCCCAAGAACCCGAAAACTTCCCAGTATTTCTTCCAGTTGCAGGAGCATTTCGTGAAAGATCTGGTCGGCCCAGGCCCCTTCACTGTTTTTGCACCTTTATCTGCAGCCTTTGATGAGGAAGCTCGGGTTAAAGACTGGGACAAATACGGTTTAATGCCCCAGGTTCTTCGGTACCATGTGGTCGCCTGCCACCAGCTGCTTCTGGAAAACCTGAAATTGATCTCAAATGCTACTTCCCTCCAAGGAGAGCCAATAGTCATCTCCGTCTCTCAGAGCACGGTGTATATAAATAATAAGGCTAAGATCATATCCAGTGATATCATCAGTACTAATGGGATTGTTCATATCATAGACAAATTGCTATCTCCCAAAAATTTGCTTATCACTCCCAAAGACAACTCTGGAAGAATTCTGCAAAATCTTACGACTTTGGCAACAAACAATGGCTACATCAAATTTAGCAACTTAATACAGGACTCAGGTTTGCTGAGTGTCATCACCGATCCCATCCACACCCCAGTCACTCTCTTCTGGCCCACCGACCAAGCCCTCCATGCCCTACCTGCTGAACAACAGGACTTCCTGTTCAACCAAGACAACAAGGACAAGCTGAAGGAGTATTTGAAGTTTCATGTGATACGAGATGCCAAGGTTTTAGCTGTGGATCTTCCCACATCCACTGCCTGGAAGACCCTGCAAGGTTCAGAGCTGAGTGTGAAATGTGGAGCTGGCAGGGACATCGGTGACCTCTTTCTGAATGGCCAAACCTGCAGAATTGTGCAGCGGGAGCTCTTGTTTGACCTGGGTGTGGCCTACGGCATTGACTGTCTGCTGATTGATCCCACCCTGGGGGGCCGCTGTGACACCTTTACTACTTTCGATGCCTCGGGGGAGTGTGGGAGCTGTGTCAATACTCCCAGCTGCCCAAGGTGGAGTAAACCAAAGGGTGTGAAGCAGAAGTGTCTCTACAACCTGCCCTTCAAGAGGAACCTGGAAGGCTGCCGGGAGCGGTGCAGCCTGGTGATACAGATCCCCAGGTGCTGCAAGGGCTACTTCGGGCGAGACTGTCAGGCCTGCCCTGGAGGACCAGATGCCCCGTGTAATAACCGGGGTGTCTGCCTTGATCAGTACTCGGCCACCGGAGAGTGTAAATGCAACACCGGCTTCAATGGGACGGCGTGTGAGATGTGCTGGCCGGGGAGATTTGGGCCTGATTGTCTGCCCTGTGGCTGCTCAGACCACGGACAGTGCGATGATGGCATCACGGGCTCCGGGCAGTGCCTCTGTGAAACGGGGTGGACAGGCCCCTCGTGTGACACTCAGGCAGTTTTGCCTGCAGTGTGTACGCCTCCTTGTTCTGCTCATGCCACCTGTAAGGAGAACAACACGTGTGAGTGTAACCTGGATTATGAAGGTGACGGAATCACATGCACAGTTGTGGATTTCTGCAAACAGGACAACGGGGGCTGTGCAAAGGTGGCCAGATGCTCCCAGAAGGGCACGAAGGTCTCCTGCAGCTGCCAGAAGGGATACAAAGGGGACGGGCACAGCTGCACAGAGATAGACCCCTGTGCAGACGGCCTTAACGGAGGGTGTCACGAGCACGCCACCTGTAAGATGACAGGCCCGGGCAAGCACAAGTGTGAGTGTAAAAGTCACTATGTCGGAGATGGGCTGAACTGTGAGCCGGAGCAGCTGCCCATTGACCGCTGCTTACAGGACAATGGGCAGTGCCATGCAGACGCCAAATGTGTCGACCTCCACTTCCAGGATACCACTGTTGGGGTGTTCCATCTACGCTCCCCACTGGGCCAGTATAAGCTGACCTTTGACAAAGCCAGAGAGGCCTGTGCCAACGAAGCTGCGACCATGGCAACCTACAACCAGCTCTCCTATGCCCAGAAGGCCAAGTACCACCTGTGCTCAGCAGGCTGGCTGGAGACCGGGCGGGTTGCCTACCCCACAGCCTTCGCCTCCCAGAACTGTGGCTCTGGTGTGGTTGGGATAGTGGACTATGGACCTAGACCCAACAAGAGTGAAATGTGGGATGTCTTCTGCTATCGGATGAAAGATGTGAACTGCACCTGCAAGGTGGGCTATGTGGGAGATGGCTTCTCATGCAGTGGGAACCTGCTGCAGGTCCTGATGTCCTTCCCCTCACTCACAAACTTCCTGACGGAAGTGCTGGCCTATTCCAACAGCTCAGCTCGAGGCCGTGCATTTCTAGAACACCTGACTGACCTGTCCATCCGCGGCACCCTCTTTGTGCCACAGAACAGTGGGCTGGGGGAGAATGAGACCTTGTCTGGGCGGGACATCGAGCACCACCTCGCCAATGTCAGCATGTTTTTCTACAATGACCTTGTCAATGGCACCACCCTGCAAACGAGGCTGGGAAGCAAGCTGCTCATCACTGCCAGCCAGGACCCACTCCAACCGACGGAGACCAGGTTTGTTGATGGAAGAGCCATTCTGCAGTGGGACATCTTTGCCTCCAATGGGATCATTCATGTCATTTCCAGGCCTTTAAAAGCACCCCCTGCCCCCGTGACCTTGACCCACACTGGCTTGGGAGCAGGGATCTTCTTTGCCATCATCCTGGTGACTGGGGCTGTTGCCTTGGCTGCTTACTCCTACTTTCGGATAAACCGGAGAACAATCGGCTTCCAGCATTTTGAGTCGGAAGAGGACATTAATGTTGCAGCTCTTGGCAAGCAGCAGCCTGAGAATATCTCGAACCCCTTGTATGAGAGCACAACCTCAGCTCCCCCAGAACCTTCCTACGACCCCTTCACGGACTCTGAAGAACGGCAGCTTGAGGGCAATGACCCCTTGAGGACACTGTGAGGGCCTGNOV9y, CG50736-24SEQ ID NO: 2562551 aaMW at 276991.0kDProtein SequenceMMLQHLVIFCLGLVVQNFCSPAETTGQARRCDRKSLLTIRTECRSCALNLGVKCPDGYTMITSGSVGVRDCRYTFEVRTYSLSLPGCRHICRKDYLQPRCCPGRWGPDCIECPGGAGSPCNGRGSCAEGMEGNGTCSCQEGFGGTACETCADDNLFGPSCSSVCNCVHGVCNSGLDGDGTCECYSAYTGPKCDKPIPECAALLCPENSRCSPSTEDENKLECKCLPNYRGDGKYCDPINPCLRKICHPHAHCTYLGPNRHSCTCQEGYRGDGQVCLPVDPCQINFGNCPTKSTVCKYDGPGQSHCECKEHYQNFVPGVGCSMTDICKSDNPCHRNANCTTVAPGRTECICQKGYVGDGLTCYGNIMERLRELNTEPRGKWQGRLTSFISLLDKAYAWPLSKLGPFTVLLPTDKGLKGFNVNELLVDNKAAQYFVKLHIIAGQMNIEYMNNTDMFYTLTGKSGEIFNSDKDNQIKLKLHGGKKKVKIIQGDIIASNGLLHILDRAMDKLEHTFESNNEQTIMTMLQPRYSKFRSLLEETNLGHALDEDGVGGPYTIFVPNNEALNNMKDGTLDYLLSPEGSRKLLELVRYHIVPFTQLEVATLISTPHIRSMANQLIQFNTTDNGQILANDVAMEEIEITAKNGRIYTLTGVLIPPSIVPILPHRCDETKREMKLGTCVSCSLVYWSRCPANSEPTALFTHRCVYSGRFGSLKSGCARYCNATVKIPKCCKGFYGPDCNQCPGGFSNPCSGNGQCADSLGGNGTCICEEGFQGSQCQFCSDPNKYGPRCNKKCLCVHGTCNNRIDSDGACLTGTCRDGSAGRLCDKQTSACGPYVQFCHIHATCEYSNGTASCICKAGYEGDGTLCSEMDPCTGLTPGGCSRNAECIKTGTGTHTCVCQQGWTGNGRDCSEINNCLLPSAGGCHDNASCLYVGPGQNECECKKGFRGNGIDCEPITSCLEQTGKCHPLASCQSTSSGVWSCVCQEGYEGDGFLCYGNAAVELSFLSEAAIFNRWINNASLQPTLSATSNLTVLVPSQQATEDMDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYPIFRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNNATTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGIHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTASCKCAAGFQGNGTICTAINACEISNGGCSAKADCKRTTPGRRVCTCKAGYTGDGIVCLEINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCSEFAICNHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKIJPKTSQYFFQLQEHFVDLVGPGPFTVFAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAEQQDFLFNQDNKDKLKEYLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNQLSYAQKAXYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRLGSKLLITASQDPLQPTETRFVDGRAILQWDIFASNGIIHVISRPLKAPPAPVTLTRTGLGAGIFFAIILVTGAVALAAYSYFRINRRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSYDPFTDSEERQLEGNDPLRTL


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 9B.

TABLE 9BComparison of the NOV9 protein sequences.NOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kMERQETGNSKTRYHATAIVQAKHDKGLNKNGTSGDEEQKIKVGDRDRENKGFDGLLDVWNNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9y------------------------------------------------------------NOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kTLNFIHPCFAVCNCVHGVCNSGLDGDGTCECYSAYTGPKCDKLTENFHTSHLTLWPVHDSNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9y------------------------------------------------------------NOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kKHWGSLRHQNMNGTCSSGGGKGDPDVYQNGLIFHGGGTSGGLSSSRNRRSSVKRPEKWKGNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9y-----------------------------------------------MMLQHLVIFCLGLNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kDDRDGGGKEGQQRRRADTESSLQRGHIKTPLPHRQGEARITETTGNCVSAGMTGTNANHTNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yVVQNFCSPAETTGQARRCDRKSLLTIRTECRSCALNLGVKCPDGYTMITSGSVGVRDCRYNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kKVHPTVQSLTEYDSFQTHSTSRLKEFEKQQVKERFSDPPLMQAIKPSHEKYPPYAQRKGTNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yTFEVRTYSLSLPGCRRICRKDYLQPRCCPGRWGPDCIECPGGAGSPCNGRGSCAEGMEGNNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kSLSPKTQGHGDDEQALLSFLHSITLSLYLYPTTFFHDSPVFIKPGIKTLRLNHFFGSSFPNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yGTCSCQEGFGGTACETCADDNLFGPSCSSVCNCVHGVCNSGLDGDGTCECYSAYTGPKCDNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kYEGSSVIXXMGIEVWKNWCQNADTLAAAPAPSLNVQPCSAQKIPDVRLPLKMKTNWNANANOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yKPIPECAALLCPENSRCSPSTEDENKLECKCLPNYRGDGKYCDPINPCLRKTCHPHAHCTNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kFPITEAMANTATPSIHVYEKSATLMLIVRTWDQIGTVVHAKKATVGMAKCACLWTPAKLTNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yYLGPNPBSCTCQEGYRGDGQVCLPVDPCQINFGNCPTKSTVCKYDGPGQSHCECKEHYQNNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kLETALQSLQCANMMGLDRCICQKGYVGDGLTCYGNIMERLRELNTEPRGKWQGRLTSFISNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yFVPGVGCSMTDICKSDNPCHRNANCTTVAPGRTECICQKGYVGDGLTCYGNIMERLRELNNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kLLESIQIVSVQLSEFSQREPTCVNTKSIASNLEGPLVPLSNHYPLQVNELLVDNKAAQYFNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yTEPRGKWQGRLTSFISLLDKAYAWPLSKLGPFTVLLPTDKGLKGFNVNELLVDNKAAQYFNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kVKLHIIAGQMNIEYMNNTDMFYTLTGKSGEIFNSDKDNQIKLKLHGGKKKVKIIQGDIIANOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yVKLHIIAGQMNIEYMNNTDMFYTLTGKSGEIFNSDKDNQIKLKLHGGKKKVKIIQGDIIANOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kSNGLLHILDRAMDKLEPTF-------------------ESNNEETNLGHALDEDGVGGPYNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9ySNGLLHILDRAMDKLEHTFESNNEQTIMTMLQPRYSKFRSLLEETNLGHALDEDGVGGPYNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kTIFVPNNEALNNMKDGTLDYLLSP------------------ELEVATLISTPHIRSMANNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yTIFVPNNEALNNMKDGTLDYLLSPEGSRKLLELVRYHIVPFTQLEVATLISTPHIRSMANNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kQLIQFNTTDNGQILANDVAMEEIEITAKNGRIYTLTGVLIPPSIVPILPHRCDETKREMKNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yQLIQFNTTDNGQILANDVAMEEIEITAKNGRIYTLTGVLIPPSIVPILPHRCDETKREMKNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kLGTCVSCSLVYWSRCPANSEPTALFTHRCVYSGRFGSLKSGCARYC--------------NOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yLGTCVSCSLVYWSRCPANSEPTALFTHRCVYSGRFGSLKSGCARYCNATVKIPKCCKGFYNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9k---------------NATVKCADSLGGNGTCICEEGFQGSQCQFCSDPNKYGPRCNKKCLNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yGPDCNQCPGGFSNPCSGNGQCADSLGGNGTCICEEGFQGSQCQFCSDPNKYGPRCNKKCLNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kCVHGTCNNRIDSDGACLTGTCRDGSAGRLCDKQTSACGPYVQFCHIHATCEYSNGTASCINOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yCVHGTCNNRIDSDGACLTGTCRDGSAGRLCDKQTSACGPYVQFCHIHATCEYSNGTASCINOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kCKAGYEGDGTLCSEMDPCTGLTPGGCSRNAECIKTGTGTHTCVCQQGWTGNGRDCSEINNNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yCKAGYEGDGTLCSEMDPCTGLTPGGCSRNAECIKTGTGTHTCVCQQGWTGNGRDCSEINNNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kCLLPSAGGCHDNASCLYVGPGQNECECKKGFRGNGIDCEPITSCLEQTGKCHPLASCQSTNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9yCLLPSAGGCHDNASCLYVGPGQNECECKKGFRGNGIDCEPITSCLEQTGKCHPLASCQSTNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kSSGVWSCVCQEGYEGDGFLCYGNAAVELSFLSEAAIFNRWINNASLQPTLSATSNLTVLVNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w------------------------------------------------------------NOV9x------------------------------------------------------------NOV9ySSGVWSCVCQEGYEGDGFLCYGNAAVELSFLSEAAIFNRWINNASLQPTLSATSNLTVLVNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kPSQQATEDMDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9w--------MDQDEKSFWLSQSNIPALIKYHNLLGTYRVADLQTLSSSDMLATSLQGNFLHNOV9x--------MDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHNOV9yPSQQATEDMDQDEKSFWLSQSNIPALIKYHMLLGTYRVADLQTLSSSDMLATSLQGNFLHNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYSIFNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYSIFNOV9xLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYSIFNOV9yLAKVDGNITIEGASIVDGDNAATNGVIHIINKVLVPQRRLTGSLPNLLMRLEQMPDYPIFNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDNOV9xRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDNOV9yRGYIIQYNLANAIEAADAYTVFAPNNNAIENYIREKKVLSLEEDVLRYHVVLEEKLLKNDNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNOV9xLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNOV9yLHNGMHRETMLGFSYFLSFFLHNDQLYVNEAPINYTNVATDKGVIHGLGKVLEIQKNRCDNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kNNDTTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wNNDTTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITNOV9xNNDTTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITNOV9yNNATTIIRGRCRTCSSELTCPFGTKSLGNEKRRCIYTSYFMGRRTLFIGCQPKCVRTVITNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGINOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGINOV9xRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGINOV9yRECCAGFFGPQCQPCPGNAQNVCFGNGICLDGVNGTGVCECGEGFSGTACETCTEGKYGINOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTNOV9xHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTNOV9yHCDQACSCVHGRCNQGPLGDGSCDCDVGWRGVHCDNATTEDNCNGTCHTSANCLTNSDGTNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kASCKCAAGFQGNGTICTAINACEISNGGCSAXADCKRTTPGRRVCTCKAGYTGDGIVCLENOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wASCKCAAGFQGNGTICTAINACEISNGGCSAXADCKRTTPGRRVCTCKAGYTGDGIVCLENOV9xASCKCAAGFQGNGTICTAINACEISNGGCSAKADCKRTTPGRRVCTCKAGYTGDGIVCLENOV9yASCKCAAGFQGNGTICTAINACEISNGGCSAKADCKRTTPGRRVCTCKAGYTGDGIVCLENOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCSEFAICNNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCGEFAICNNOV9xINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCGEFAICNNOV9yINPCLENHGGCDKNAECTQTGPNQAACNCLPAYTGDGKVCTLINVCLTKNGGCSEFAICNNOV9a------------------------------------------------------------NOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e----MEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERNEDREAKWHIAAKDSNOV9f----MEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERMEDRRAKWHIAAKDSNOV9g------------------------------------------------------------NOV9h----MEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERNEDREAKWHIAAKDSNOV9i----MEARKELESLPPFCLNSLHSIILTTKLTSQSLGGPRGVEERMEDRPAKWHIAAKDSNOV9j------------------------------------------------------------NOV9kHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKNPKTSQYFFQLQEHFVKDLVGPGPFTVFNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9g------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKNPKTSQYFFQLQEHFVKDLVGPGPFTVFNOV9xHTGQVERTCTCKPNYIGDGFTCRGSIYQELPKNPKTSQYFFQLQEHFVKDLVGPGPFTVFNOV9yHTGQVERTCTCKPNYIGDGFTCRGSIYQELFKNPKTSQYFFQLQEHFVKDLVGPGPFTVFNOV9a---------------------MPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9eCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9fCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9g------------------------------------------------------------NOV9hCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9iCLWLKPSDLLLQVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9j------------------------VLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9kAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9xAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9yAPLSAAFDEEARVKDWDKYGLMPQVLRYHVVACHQLLLENLKLISNATSLQGEPIVISVSNOV9aQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9b-------------------------------------------GSRILQNLTTLATNNGYNOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9eQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9fQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9g------------------------------------------------------------NOV9hQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9iQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9jQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9kQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9l---------------------------------------------RILQNLTTLATNNGYNOV9m---------------------------------------------RILQNLTTLATNNGYNOV9n---------------------------------------------RILQNLTTLATNNGYNOV9o---------------------------------------------RILQNLTTLATNNGYNOV9p---------------------------------------------RILQNLTTLATNNGYNOV9q---------------------------------------------RILQNLTTLATNNGYNOV9r---------------------------------------------RILQNLTTLATNNGYNOV9s---------------------------------------------RILQNLTTLATNNGYNOV9t---------------------------------------------RILQNLTTLATNNGYNOV9u---------------------------------------------RILQNLTTLATNNGYNOV9v---------------------------------------------RILQNLTTLATNNGYNOV9wQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9xQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9yQSTVYINNKAKIISSDIISTNGIVHIIDKLLSPKNLLITPKDNSGRILQNLTTLATNNGYNOV9aIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9bIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9eIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9fIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9g------------------------------------------------------------NOV9hIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9iIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9jIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9kIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALHALPAEQQDFLFNQDNKDKLKEYNOV9lIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9mIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9nIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9oIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9pIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9qIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9rIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9sIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9tIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9uIKFSNLIQDSGLLSVVTDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9vIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9wIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9xIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9yIKFSNLIQDSGLLSVITDPIHTPVTLFWPTDQALHALPAE---QQDFLFNQDNKDKLKEYNOV9aLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9bLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9eLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9fLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9g---------------LPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9hLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9iLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9jLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9kLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTYRIVQRELLFDNOV9lLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9mLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9nLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9oLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9pLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9qLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9rLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOv9sLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9tLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9uLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9vLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9wLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9xLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9yLKFHVIRDAKVLAVDLPTSTAWKTLQGSELSVKCGAGRDIGDLFLNGQTCRIVQRELLFDNOV9aLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9bLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9eLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9fLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9gLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9hLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9iLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9jLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9kLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9lLGVAYGIDCLLIDPTLGGRCDTFTTFDAS-------------------GVKQKCLYNLPFNOV9mLGVAYGIDCLLIDPTLGGRCDTFTTFDAS-------------------GVKQKCLYNLPFNOV9nLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9oLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9pLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9qLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9rLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9sLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQRCLYNLPFNOV9tLGVACGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9uLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9vLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9wLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9xLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9yLGVAYGIDCLLIDPTLGGRCDTFTTFDASGECGSCVNTPSCPRWSKPKGVKQKCLYNLPFNOV9aKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTNOV9bKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTNOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9eKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPVAPCNNRGVCLDQYSATGECKCNTNOV9fKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTNOV9gKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTNOV9hKRNLEGCRERCSLVIQIPRCCKGYFGRDCQGE--GASSP-------LATL----KVSALINOV9iKRNLEGCRERCSLVIQIP------------------------------------------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--------NSKSVCLDQYSATGECKCNTNOV9xKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTNOV9yKRNLEGCRERCSLVIQIPRCCKGYFGRDCQACPGGPDAPCNNRGVCLDQYSATGECKCNTNOV9aGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVNOV9bGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLPAVNOV9c--------MCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVNOV9d--------MCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVNOV9eGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVNOV9fGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVNOV9gGFNGTACEMCWPGRFGPDCLPCGCSDHGQCDDGITGSGQCLCETGWTGPSCDTQAVLSAVNOV9hSTRPPESVNATPASMGRRVRCAGRGDLGLIVCPVA-A--------------QTTDSAMMANOV9i---------SLPWRTRCPV-----------------------------------------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------------GQAPRVTLRQFCLQCVRLLVLLMPPVRRTTRVSVTWNOV9i------------------------------------------------------------NOV9jCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9kCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9lCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9mCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9nCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9oCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9pCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9qCTPPCSAHATCKENNTCECYLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9rCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCNCNOV9sCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9tCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9uCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9vCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9wCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9xCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9yCTPPCSAHATCKENNTCECNLDYEGDGITCTVVDFCKQDNGGCAKVARCSQKGTKVSCSCNOV9aQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9bQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCGPEQLPNOV9cQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9dQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9eQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9fQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9gQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9hIMKVT---ESHAQLWISANRTTGAVQRWPDAPRPAR-RSPAAARRDTKGTGTAAQR----NOV9i------------------------------------------------------------NOV9jQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9kQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9lQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9mQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9nQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9oQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9pQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKNTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9qQKGYKGDGHSCTEIDPCADGLNGGCHEHATRKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9rQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9sQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9tQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKNTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9uQKGYKGDGHSCTETDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9vQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSRYVGDGLNCEPEQLPNOV9wQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9xQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9yQKGYKGDGHSCTEIDPCADGLNGGCHEHATCKMTGPGKHKCECKSHYVGDGLNCEPEQLPNOV9aIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9bIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9cIDRCLQDNGQCHADAKCADLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9dIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9eIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9fIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9gIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9jIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9kIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9lIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9mIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9nIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDRAREACANEAATMATYNOV9oIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9pIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9qIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9rIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9sIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9tIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9uIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATTATYNOV9vIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9wIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9xIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9yIDRCLQDNGQCHADAKCVDLHFQDTTVGVFHLRSPLGQYKLTFDKAREACANEAATMATYNOV9aNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9bNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9cNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9dNQLSYAQKREEK------------------------------------------------NOV9eNQLSYAQKTWYSFTKE--------------------------------------------NOV9fNQLSYAQKTWYSFTKE--------------------------------------------NOV9gNQLSYAQKTWYSFTKE--------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9jNQLSYAQKTWYSFTKE--------------------------------------------NOV9kNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9lNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9mNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9nNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9oNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9pNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9qNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9rNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9sNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSSVVGIVDYGPRPNKSEMWDVFCYNOV9tNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9uNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9vNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9wNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9xNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9yNQLSYAQKAKYHLCSAGWLETGRVAYPTAFASQNCGSGVVGIVDYGPRPNKSEMWDVFCYNOV9aRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDNOV9bRP----------------------------------------------------------NOV9cRMKGS----------------AGLFQQLSSRPCISRTPD---------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9kRMKGS----------------AGLFQQLSSRPCISRTPD---------------------NOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDNOV9xRMKDVNCTCKVGYVGDGFSCSGNLLQVLNSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDNOV9yRMKDVNCTCKVGYVGDGFSCSGNLLQVLMSFPSLTNFLTEVLAYSNSSARGRAFLEHLTDNOV9aLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRVGSKLLITASNOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9k------------------------------------------------------------NOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRVGSKLLITASNOV9xLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRLGSKLLITASNOV9yLSIRGTLFVPQNSGLGENETLSGRDIEHHLANVSMFFYNDLVNGTTLQTRLGSKLLITASNOV9aQDPLQPTETRFVDGRAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILNOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9k------------------------------------------------------------NOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wQDPLQPTETRFVDGRAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILNOV9xQDPLQPTETRFVDGPAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILNOV9yQDPLQPTETRFVDGRAILQWDIFASNGIIHVISRPLKAPPAPVTLTHTGLGAGIFFAIILNOV9aVTGAVALAAYSYFRINRRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSNOV9b------------------------------------------------------------NOV9c------------------------------------------------------------NOV9d------------------------------------------------------------NOV9e------------------------------------------------------------NOV9f------------------------------------------------------------NOV9g------------------------------------------------------------NOV9h------------------------------------------------------------NOV9i------------------------------------------------------------NOV9j------------------------------------------------------------NOV9k------------------------------------------------------------NOV9l------------------------------------------------------------NOV9m------------------------------------------------------------NOV9n------------------------------------------------------------NOV9o------------------------------------------------------------NOV9p------------------------------------------------------------NOV9q------------------------------------------------------------NOV9r------------------------------------------------------------NOV9s------------------------------------------------------------NOV9t------------------------------------------------------------NOV9u------------------------------------------------------------NOV9v------------------------------------------------------------NOV9wVTGAVALAAYSYFRINRRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSNOV9xVTGAVALAAYSYFRINRRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSNOV9yVTGAVALAAYSYFRINRRTIGFQHFESEEDINVAALGKQQPENISNPLYESTTSAPPEPSNOV9aYDPFTDSEERQLEGNDPLRTLNOV9b---------------------NOV9c---------------------NOV9d---------------------NOV9e---------------------NOV9f---------------------NOV9g---------------------NOV9h---------------------NOV9i---------------------NOV9j---------------------NOV9k---------------------NOV9l---------------------NOV9m---------------------NOV9n---------------------NOV9o---------------------NOV9p---------------------NOV9q---------------------NOV9r---------------------NOV9s---------------------NOV9t---------------------NOV9u---------------------NOV9v---------------------NOV9wYDPFTDSEERQLEGNDPLRTLNOV9xYDPFTDSEERQLEGNDPLRTLNOV9yYDPFTDSEERQLEGNDPLRTLNOV9a(SEQ ID NO: 208)NOV9b(SEQ ID NO: 210)NOV9c(SEQ ID NO: 212)NOV9d(SEQ ID NO: 214)NOV9e(SEQ ID NO: 216)NOV9f(SEQ ID NO: 218)NOV9g(SEQ ID NO: 220)NOV9h(SEQ ID NO: 222)NOV9i(SEQ ID NO: 224)NOV9j(SEQ ID NO: 226)NOV9k(SEQ ID NO: 228)NOV9l(SEQ ID NO: 230)NOV9m(SEQ ID NO: 232)NOV9n(SEQ ID NO: 234)NOV9o(SEQ ID NO: 236)NOV9p(SEQ ID NO: 238)NOV9q(SEQ ID NO: 240)NOV9r(SEQ ID NO: 242)NOV9s(SEQ ID NO: 244)NOV9t(SEQ ID NO: 246)NOV9u(SEQ ID NO: 248)NOV9v(SEQ ID NO: 250)NOV9w(SEQ ID NO: 252)NOV9x(SEQ ID NO: 254)NOV9y(SEQ ID NO: 256)


Further analysis of the NOV9a protein yielded the following properties shown in Table 9C.

TABLE 9CProtein Sequence Properties NOV9aSignalP analysis:Cleavage site between residues 32 and 33PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos. chg 1; neg. chg 0H-region:length 11; peak value 3.91PSG score:−0.49GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −5.51possible cleavage site: between 28 and 29>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 808-824−8.33PERIPHERALLikelihood = 2.97 (at 663)ALOM score: −8.33 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 815Charge difference: −1.0 C(1.5)-N(2.5)N >= C: N- terminal side will be inside>>> Single TMS is located near the C-terminus>>> membrane topology: type Nt (cytoplasmic tail 1 to 807)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):5.98Hyd Moment(95):9.18G content:0D/E content:1S/T content:0Score: −3.74Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 16 LRY|HVNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.7%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 15LL at 16LL at 69LL at 75LL at 111LL at 213LL at 226LL at 660checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic /Nuclear discriminationPrediction: nuclearReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):30.4%: nuclear21.7%: cytoplasmic13.0%: mitochondrial13.0%: Golgi 8.7%: vesicles of secretory system 8.7%: endoplasmic reticulum 4.3%: peroxisomal>> prediction for CG50736-09 is nuc (k = 23)


A search of the NOV9a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 9D.

TABLE 9DGeneseq Results for NOV9aNOV9aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABJ10588Human novel protein NOV1c SEQ ID1 . . . 897 897/897 (100%)0.0NO: 211 - Homo sapiens, 897 aa.1 . . . 897 897/897 (100%)[WO200259315-A2, 01-AUG-2002]ABG72514Human 190 kDa Hyaluronan receptor1 . . . 897896/897 (99%)0.0for endocytosis (HARE) #2 - Homo757 . . . 1653 897/897 (99%)sapiens, 1653 aa. [WO200286093-A2,31-OCT-2002]ABG72499Human 190 kDa Hyaluronan receptor1 . . . 897896/897 (99%)0.0for endocytosis (HARE) #1 - Homo520 . . . 1416 897/897 (99%)sapiens, 1416 aa. [WO200286093-A2,31-OCT-2002]AAM47684Human Hyaluronic Acid Receptor for1 . . . 897896/897 (99%)0.0Endocytosis, HARE - Homo sapiens,498 . . . 1394 897/897 (99%)1394 aa. [WO200181544-A2, 01-NOV-2001]ABG72498Rat 175 kDa Hyaluronan receptor for1 . . . 886674/887 (75%)0.0endocytosis (HARE) - Rattus sp, 1431519 . . . 1404 758/887 (84%)aa. [WO200286093-A2, 31-OCT-2002]


In a BLAST search of public sequence databases, the NOV9a protein was found to have homology to the proteins shown in the BLASTP data in Table 9E.

TABLE 9EPublic BLASTP Results for NOV9aNOV9aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9NRY3CD44-like precursor FELL - Homo1 . . . 897 897/897 (100%)0.0sapiens (Human), 897 aa.1 . . . 897 897/897 (100%)AAO39681Hyaluronan receptor for1 . . . 897896/897 (99%)0.0endocytosis - Homo sapiens520 . . . 1416 897/897 (99%)(Human), 1416 aa (fragment).Q8IUG9FELE-2 - Homo sapiens (Human),1 . . . 897896/897 (99%)0.02551 aa.1655 . . . 2551 897/897 (99%)Q8WWQ8Stabilin-2 - Homo sapiens (Human),1 . . . 897896/897 (99%)0.02551 aa.1655 . . . 2551 897/897 (99%)Q9H7H7FLJ00112 protein - Homo sapiens1 . . . 897896/897 (99%)0.0(Human), 1192 aa (fragment).296 . . . 1192 897/897 (99%)


PFam analysis predicts that the NOV9a protein contains the domains shown in the Table 9F.

TABLE 9FDomain Analysis of NOV9aIdentities/PfamNOV9aSimilaritiesforExpect0 DomainMatch Regionthe Matched RegionValueFasciclin 86 . . . 22938/152 (25%) 1.5e−08100/152 (66%) EGF355 . . . 38713/47 (28%)0.2422/47 (47%)EGF397 . . . 42613/47 (28%)0.05423/47 (49%)EGF432 . . . 46710/47 (21%)0.06623/47 (49%)EGF473 . . . 51013/47 (28%)0.03631/47 (66%)Xlink543 . . . 63640/104 (38%) 1.1e−2669/104 (66%) 


Example 10

The NOV10 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 10A.

TABLE 10ANOV10 Sequence AnalysisNOV10a, CG50925-01SEQ ID NO: 2577892 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 4627ATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTGCCGTGGCTCCTGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTCGGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGCGGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTTGGCAACCGTTTCTCCACTGCCCCTTGCCCGCAGTCGGGGCTGGCCCAGTCTTTTCGATTGTGTCCAGAACAATACAGTAACGCCTCTGGTTGGGTGAGTTCAGAGGCTGCCCGCTTCCTGACAGGACACAACTGGTCAGCCTCTGACGCTGGCCGCCCGGCCTGCATTTTCCCCGGCCCGGTCTCCTGGAGACAGGTACCAGCTTACAACGCCGGACAGGATCAGGGTAGCAAATATAGCTTCTATGGAGGAAAGGTCAAAGATGAGTATCTGCGTAGGTCCCCGATCTGTCGCTCTGCTGCCGCCCTGCACACTGACACCAGTGCCCGCAGCTGGTCTGAATTTAGGCTCTTGAGCAATAACAAGATCACGGGGCTCCGCAATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTTCTCATGAGAGCACATTTTTCTGATGAAGATGAAAAGACGGCCTGTCCTTCTCCAGCACCAAGGCTCTGGAGGCTGCATAGCAAGTGCACCCTGAGCATGGCAGGCAATCAGAAGAACCAGGGCGGCAGCAGCTCACAAGAGGCAGCATCTGGCTTGATGCCGAGTCGGCGGCTCCAGCGGGGAGAGGTTGAGGGCCTCTGCACTTGCCTCTGCACGGACCTGAGGAACAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCATGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATGCCCAGCAACTTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCATAGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCACCCCGTGGTATACCCCTGCACGGCCTTGCTGCTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCAACCACAGCTCCATCCGTGTGTCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCATGACCTCTGCTGTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATCACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTCCATAAGGAGCTCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACTCCCAGTCCTATGCTCCGGTACATACTTTCAATTCCAGCTTTGCAATTGGGGAGGGACTCCAACGCAGGCGTAGGAAACCTCCCAAGGTTCTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGCATCACAGCTGCAGTCAACATCCACAACTACCGGGACCACAGCCCCTACTGCTGGCTGGTGTGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTTTGATTCTGCTCATCACCTGGATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAACCCCAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGGGGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTGGGGACGCCCGGGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGGGCGCTGGTGACCACGCACTTCCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTGTCCCAGCGCTGGCTGCCCCGGGTGGTGTGCAGCTGCTTGTACGGGGTGGCAGCCTCCGCCCTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGAGGGACGTGAGAGCCTCGTGGCGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCGGGCCCTGCCCGCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGGCCCCCCTCCCTCAAGTCCTCCCCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAGCTGGCCCAGAGTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAGCCGGCGGGCACCCGGGGAAACCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGTCGGGCGCACAAGAGCCGGGCCAAGGGACACCGCGCGGGGGAGGCCTGCGGCAAGAACCGGCTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAACTGCTGTCCAGCGAGAGCGGTAGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAGCCCGGGCGCCGGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCCGCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTCAAGGGCGGCGGCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCCAGCCTAAACGGCGCCCCCAAGGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAGGTAGCCAGCGGCGGCTGCATGAAGACCGGACTCTGGAAGAGCGAAACTACCGTCTAAGGTGGGGCGGGCGACGCGGTAGACGGGTTGGCCACGCGGCTCGTTCCCCCGCTCCTCGGGGCCCTCCAAGGTGTCTCCGTAGTCAGCAGGTTGGAGGCAGAGGAGCCGATGGCTGGAGGAAGCCCACAGGCGGATGTTCCCCACTTGCCTAGAGGGCATCCCTCTGGGGTAGCGACAGACAATCCCAGAAACACGCATAATACATTTCCGTCCAGCCCGGGGCAGTCTGACTGTCGGTGCCCTCCCAGGAACGGGGAAGGCCTCCGTCTGTGTGAAAGGGCACAGCACATCCCAGGTGCACCCTCCCCAAGTACTCCCACCCCGCCTACTGTCCATGCGGCCTCACTGGGGGCCATCAGCCTCACCAGCAAAGCAGAGATGAGAGCGTGGGAACTGTGTTCTTTCCTCCCTGCCCTCTACTGATTTCAGCCCAGCCCCTGCCTAGATCCTAGGTCCCTTTTCCTCCCGAGTTTGGCTGGCACGAGAGCTAGCCCAGCACATGAAGCAGGTGATGTTAAGTCACAAGGTGCTGCTTTTCAGATCCACTATGCAAGAGGGGAGGGTGGGGCCACGTGAAAGGCAGCTCTAGACATCAACCAGTCCTGGGGGAGGGGAGTGGGAACCGGGCACAACTAGGAACAATGCCACCATTCCCACAGGAGTGGTACTTAAACCAGACAGCAGGGTTCAGAGGTGGCACACCGGGACAAAGCTGAGGCCCTGCACCTCAACAGCTGACTGCCAGGTGCCTGTGGGTGAACTGAGGGGAGTAGAGGGAGAGGGCAGGTGGAACTGGGGCAGAATCTAGTCATGCCCTAAAGCTAGTCCTGTAAACAATGGTGCCCCAGAAAGCTGCAGGTGGTGTTTGGAGAAGCAGTTACTTTTCAGTTACAAGACCCATCTCCCTAGTCTCAGCCTTACAACACCACGGGACTAAGGAAGAGCACTTCCTTGCCTCCGTAAGGCCAGAGGAAGAACCATCCCAATCATTTGATCTCCAGCTCCACAGTAGAGAGAAACCTACAAAATGTCAAACCAGCTTCCCGACTCCCAGGAGCTCAAGCCAAGCCCAGAGGCAGTGGCTGGGGTCCCTGCAGGTCATGAGGGGCCTATGCCTTTACTCCTTTTAAACACCAGCACCCGTCTTTTCCCCAACCTAAAACCAACCACCAGCATTTCACTACAGGACCAAATGGAAACCGAGGGAACCCTGGGTCTTGGGAAGAACAACAGGAAACCAAGGTCTGACCTAGGGTTCCCTCCCAGTCTTCACATCACTCTGGCCTCATCACCAAGGTGACAGAGGACACAGGGGAGGGGGAAAACCCACACACACTCCTTGGAATGGGTCCTGTTATTTATGCTTGCTGCACAGACATATTAGAAGAAAAAAAAAAAGCTTTGTATTATTCTTCCACATATGCTGGCTGCTGTTTACACACCCTGCCAATGCCTTAGCACTGGAGAGCTTTTTGCAATATGCTGGGGAAAGGGGAGGGAGGGAATGAAAGTGCCAAAGAAAACATGTTTTTAAGAACTCGGGTTTTATACAATAGAATGTTTTCTAGCAGATGCCTCTTGTTTTAATATATTAAAATTTTGCAAAGCCCTTTGAGCTACTGCCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACACCATACACACAAACCCATCATTGCCTGTGAATGCACGTAGGGCCAGAATTCCCCAGTTCCCGCTCCTCTGAGGGTTGATACTGCTGGGAATGCCAACCACTCCACAAGCAGAGGGAAGCCCCCTCAGGCCTGCAGGAGGAGCCGCAGCAGTGTGTCCAATTCAAACCAGCAGCAAAGAGCCTGACATTTTCCCATCCATCTATGAGGAAAGCCATCTCACAGAACATGGACATAGGCAACTTGCTCTCCCACACCAAGGGATGGGAATCTCTCCTACCTATAGTCATCCCTGCACTCCTGACTTTACTCCAGGACCCAGGGTCCAACTAATGGCAGAGCCCCTCTTGGTTCCTTCAAACAAGAAAAGCAATACCTACGGACTGGTGTACACTTCCATCCTTGGTTATAACAGGAATGTTATCAAGCTGTCAGAACAGGATGAAGTGCTCCCAGTGGATATCCATCAGGGAGGGTTAGGGACACTCGTGGCAGCCTGTCTAGCAGCCTGGGCTCTCTGAAAGTCCCTAACTTCCTGAGGGGTACGCAAATACTGTTCTATTTCACTATCAGAAATGTTCTCATCTCCAGTGACAGTGGAGACAGGGGGTACAGGGCAGATCCGCTTCGGGGACTTCAACATGCAGGGTGGCAAGAGAAGGGCAGGACTGGCCGGCCGCTTCCCCTGGGGTAAACCTAAGGAATTATTTCCCACCTCCCCTTCTCCTTGCCCCTGTCCCCACCCCGGTGGCTCCTTCTCTCGGGTCTCCACTTCTGCTGTCCCATCCCGAAAGGCAGAGCGGACCAGTGACTGGCGGTGCTGGAGAAGGTCACCGATGTGCTTCACCACAGACCGTTTGTCAAGTCTCAGAACTCGTAACCAGGCCAGCTGCTCAGCCATCCGCAGCAGCACAGCCAGCAGCTCCTGCAGGCGGGAGGACGCCGGGTAGGGCAGGTCCACATTTGCCAATTTACAAAATCGGGCAAGGGAACATGAAAGCCGATCTGCAGGCTGCAGCGACTGCCAAGCCAGGAAAGTCGCAGCAGTGATGACGGGCAAGGGATGCCTCCCGGTCACCAGCCACGTCTCATTTGCCAGCTCCACCAACTGCATTGTTCGAGACAGCATCTTCTCTTTGTCTTCCACGTATTTGGCTGGCACAGAAGGTGAAGCTTGGAACAGTTTGAAGCTGAAATAACCAAAATGAGGGTTGGATCTTAATGATATAGGGGCTGCTCTCCCACAGTGAGGAAAGACAGCCCACTCAAGATGGGGAAGCTATTCTGCCCTCAGGAATACTCAAGCTCACTGGGCAGCAAGTTAATAAAGGTAGTGAGAGAAAACAGGGCGTCTTCCGCTTGTTAGGGGAAGGTGGAGGGATGGAGGAGAGCACGAACATTTATTGGGCGCCTCCCAATCACCATTATTCTGAGTGCTTTACAACGTTCTCATTTAATCTACGTGCACGTGCACCATCTTATGTGCATGTATAGTTAAAAAACTTTCCCATAGTCATCCAGCCAGGCAGTAACCAAGCTTCAAATACAAGGCTATTTGACACCAACAGCCTCTACTTTCAACGTTATTTATCAGNOV10a, CG50925-01SEQ ID NO: 2581542 aaMW at 164901.2kDProtein SequenceMRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLGNRFSTAPCPQSGLAQSFRLCPEQYSNASGWVSSEAARFLTGHNWSASDAGRPACIFPGPVSWRQVPAYNAGQDQGSKYSFYGGKVKDEYLRRSPICRSAAALHTDTSARSWSEFRLLSNNKITGLRNGSFLGLSLLEKLLMRAHFSDEDEKTACPSPAPRLWRLHSKCTLSMAGNQKNQGGSSSQEAASGLMPSRRLQRGEVEGLCTCLCTDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAHALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDNMDVVYVAQMIQKFLGYVDQIKELVEVMVDMPSNLMLVDEHLLWLAQREDKACSRIVGAIERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGLHPVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWRAPPPQEGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAGEELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPAAPHAPPRALPAAAEDGSPVFGEGPPSLKSSPSGSSGHPLALGPCKLTNLQLAQSQVCEAGAAAGGEGEPEPAGTRGNLAHRHPNNVHHGRRAHKSRAKGHRAGEACGKNRLKALRGGAAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRAGQRRSASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKTGLWKSETTVNOV10b, CG50925-08SEQ ID NO: 2594103 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 3991ATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTGCCGTGGCTCCTGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTCGGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGCGGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTTGACCTGAGGAACAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCATGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGTCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATGGCCAGCAACTTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCCTGGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGTTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCACCCCGTGGTATACCCCTGCACGGCCTTGCTGCTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCAACCACAGCTCCATCCGTGTGTCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCATGACCTCTGCTGTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATCACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTCCATAAGGAGCTCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACTCCCAGTCCTATGCTCCGGTACATACTTTCAATTCCAGCTTTGCAATTGGGGAGGGACTCCAACGCAGGCGTAGGAAACCTCCCAAGGTTCTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGCATCACAGCTGCAGTCAACATTCACAACTACCGGGACCACAGCCCCTACTGCTGGCTGGTGTGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTTTGATTCTGCTCATCACCTGGATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAACCCCAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGGGGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTGGGGACGCCCGGGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGGGCGCTGGTGACCACGCACTTCCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTGTCCCAGCGCTGGCTGCCCCGGGTGGTGTGCAGCTGCTTGTACGGGGTGGCAGCCTCCGCCCTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGAGGGACGTGAGAGCCTCGTGGCGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCGGGCCCTGCCCGCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGGCCCCCCTCCCTCAAGTCCTCCCCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAGCTGGCCCAGAGTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAGCCGGCGGGCACCCGGGGAAACCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGTCGGGCGCACAAGAGCCGGGCCAAGGGACACCGCGCGGGGGAGGCCTGCGGCAAGAACCGGCTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAACTGCTGTCCAGCGAGAGCGGTAGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAGCCCGGGCGCCGGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCCGCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTCAAGGGCGGCGGCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCCAGCCTAAACGGCGCCCCCAAGGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAGGTAGCCAGCGGCGGCTGCATGAAGACCGGACTCTGGAAGAGCGAAACTACCGTCTAAGGTGGGGCGGGCGACGCGGTAGACGGGTTGGCCACGCGGCTCGTTCCCCCGCTCCTCGGGGCCCTCCAAGGTGTCTCCGTAGTCAGCAGGTTGGAGGCAGAGGAGCCGATNOV10b, CG50925-08SEQ ID NO: 2601330 aaMW at 141782.5kDProtein SequenceMRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAHALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWESGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGLHPVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWEAPPPQEGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAGEELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPAAPHAPPRALPAAAEDGSPVFGEGPPSLKSSPSGSSGHPLALGPCKLTNLQIAQSQVCEAGAAAGGEGEPEPAGTRGNLAHRHPNNVHHGRRAHKSRAKGHRAGEACGKNRLKALRGGAAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRAGQRRSASRDSLKGGGALEKESHPRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKTGLWKSETTVNOV10c, 226990087SEQ ID NO: 261341 bpDNA SequenceORF Start: at 3ORF Stop: end of sequenceCAACCGGATCCGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCAGGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGCTCGAGNOV10c, 226990087SEQ ID NO: 262113 aaMW at 12539.2kDProtein SequenceTGSELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAQALGSLQEAQLCCELENOV10d, 320054488SEQ ID NO: 2632227 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTCGGGGGAGCGGGCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGCGGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTGCTCTTGAGCAATAACAAGATCACGGGGCTCCGCAATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTGGACCTGAGGAACAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCAGGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACGACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATGGCCAGCAACCTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCCTGGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCTCGAGGGCNOV10d, 320054488SEQ ID NO: 264742 aaMW at 79823.1kDProtein SequenceTGSAPGCPLSIRSCKCSGEPAKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLLLSNNKITGLRNGSFLGLSLLEKLDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAQALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHDRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDPAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGLLEGNOV10e, CG50925-02SEQ ID NO: 2657860 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 4627ATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTGCCGTGGCTCCTGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTCGGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGCGGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTTGGCAACCGTTTCTCCACTGCCCCTTGCCCGCAGTCGGGGCTGGCCCAGTCTTTTCGATTGTGTCCAGAACAATACAGTAACGCCTCTGGTTGGGTGAGTTCAGAGGCTGCCCGCTTCCTGACAGGACACAACTGGTCAGCCTCTGACGCTGGCCGCCCGGCCTGCATTTTCCCCGGCCCGGTCTCCTGGAGACAGGTACCAGCTTACAACGCCGGACAGGATCAGGGTAGCAAATATAGCTTCTATGGAGGAAAGGTCAAAGATGAGTATCTGCGTAGGTCCCCGATCTGTCGCTCTGCTGCCGCCCTGCACACTGACACCAGTGCCCGCAGCTGGTCTGAATTTAGGCTCTTGAGCAATAACAAGATCACGGGGCTCCGCAATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTTCTCATGAGAGCACATTTTTCTGATGAAGATGAAAAGACGGCCTGTCCTTCTCCAGCACCAAGGCTCTGGAGGCTGCATAGCAAGTGCACCCTGAGCATGGCAGGCAATCAGAAGAACCAGGGCGGCAGCAGCTCACAAGAGGCAGCATCTGGCTTGATGCCGAGTCGGCGGCTCCAGCGGGGAGAGGTTGAGGGCCTCTGCACTTGCCTCTGCACGGACCTGAGGAACAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCATGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATGCCCAGCAACTTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCATAGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCACCCCGTGGTATACCCCTGCACGGCCTTGCTGCTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCAACCACAGCTCCATCCGTGTGTCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCATGACCTCTGCTGTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATCACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTCCATAAGGAGCTCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACTCCCAGTCCTATGCTCCGGTACATACTTTCAATTCCAGCTTTGCAATTGGGGAGGGACTCCAACGCAGGCGTAGGAAACCTCCCAAGGTTCTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGCATCACAGCTGCAGTCAACATCCACAACTACCGGGACCACAGCCCCTACTGCTGGCTGGTGTGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTTTGATTCTGCTCATCACCTGGATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAACCCCAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGGGGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTGGGGACGCCCGGGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGGGCGCTGGTGACCACGCACTTCCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTGTCCCAGCGCTGGCTGCCCCGGGTGGTGTGCAGCTGCTTGTACGGGGTGGCAGCCTCCGCCCTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGAGGGACGTGAGAGCCTCGTGGCGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCGGGCCCTGCCCGCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGGCCCCCCTCCCTCAAGTCCTCCCCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAGCTGGCCCAGAGTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAGCCGGCGGGCACCCGGGGAAACCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGTCGGGCGCACAAGAGCCGGGCCAAGGGACACCGCGCGGGGGAGGCCTGCGGCAAGAACCGGCTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAACTGCTGTCCAGCGAGAGCGGTAGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAGCCCGGGCGCCGGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCCGCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTCAAGGGCGGCGGCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCCAGCCTAAACGGCGCCCCCAAGGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAGGTAGCCAGCGGCGGCTGCATGAAGACCGGACTCTGGAAGAGCGAAACTACCGTCTAAGGTGGGGCGGGCGACGCGGTAGACGGGTTGGCCACGCGGCTCGTTCCCCCGCTCCTCGGGGCCCTCCAAGGTGTCTCCGTAGTCAGCAGGTTGGAGGCAGAGGAGCCGATGGCTGGAGGAAGCCCACAGGCGGATGTTCCCCACTTGCCTAGAGGGCATCCCTCTGGGGTAGCGACAGACAATCCCAGAAACACGCATAATACATTTCCGTCCAGCCCGGGGCAGTCTGACTGTCGGTGCCCTCCCAGGAACGGGGAAGGCCTCCGTCTGTGTGAAAGGGCACAGCACATCCCAGGTGCACCCTCCCCAAGTACTCCCACCCCGCCTACTGTCCATGCGGCCTCACTGGGGGCCATCAGCCTCACCAGCAAAGCAGAGATGAGAGCGTGGGAACTGTGTTCTTTCCTCCCTGCCCTCTACTGATTTCAGCCCAGCCCCTGCCTAGATCCTAGGTCCCTTTTCCTCCCGAGTTTGGCTGGCACGAGAGCTAGCCCAGCACATGAAGCAGGTGATGTTAAGTCACAAGGTGCTGCTTTTCAGATCCACTATGCAAGAGGGGAGGGTGGGGCCACGTGAAAGGCAGCTCTAGACATCAACCAGTCCTGGGGGAGGGGAGTGGGAACCGGGCACAACTAGGAACAATGCCACCATTCCCACAGGAGTGGTACTTAAACCAGACAGCAGGGTTCAGAGGTGGCACACCGGGACAAAGCTGAGGCCCTGCACCTCAACAGCTGACTGCCAGGTGCCTGTGGGTGAACTGAGGGGAGTAGAGGGAGAGGGCAGGTGGAACTGGGGCAGAATCTAGTCATGCCCTAAAGCTAGTCCTGTAAACAATGGTGCCCCAGAAAGCTGCAGGTGGTGTTTGGAGAAGCAGTTACTTTTCAGTTACAAGACCCATCTCCCTAGTCTCAGCCTTACAACACCACGGGACTAAGGAAGAGCACTTCCTTGCCTCCGTAAGGCCAGAGGAAGAACCATCCCAATCATTTGATCTCCAGCTCCACAGTAGAGAGAAACCTACAAAATGTCAAACCAGCTTCCCGACTCCCAGGAGCTCAAGCCAAGCCCAGAGGCAGTGGCTGGGGTCCCTGCAGGTCATGAGGGGCCTATGCCTTTACTCCTTTTAAACACCAGCACCCGTCTTTTCCCCAACCTAAAACCAACCACCAGCATTTCACTACAGGACCAAATGGAAACCGAGGGAACCCTGGGTCTTGGGAAGAACAACAGGAAACCAAGGTCTGACCTAGGGTTCCCTCCCAGTCTTCACATCACTCTGGCCTCATCACCAAGGTGACAGAGGACACAGGGGAGGGGGAAAACCCACACACACTCCTTGGAATGGGTCCTGTTATTTATGCTTGCTGCACAGACATATTAGAAGAAAAAAAAAAAGCTTTGTATTATTCTTCCACATATGCTGGCTGCTGTTTACACACCCTGCCAATGCCTTAGCACTGGAGAGCTTTTTGCAATATGCTGGGGAAAGGGGAGGGAGGGAATGAAAGTGCCAAAGAAAACATGTTTTTAAGAACTCGGGTTTTATACAATAGAATGTTTTCTAGCAGATGCCTCTTGTTTTAATATATTAAAATTTTGCAAAGCCCTTTGAGCTACTGCCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACACCATACACACAAACCCATCATTGCCTGTGAATGCACGTAGGGCCAGAATTCCCCAGTTCCCGCTCCTCTGAGGGTTGATACTGCTGGGAATGCCAACCACTCCACAAGCAGAGGGAAGCCCCCTCAGGCCTGCAGGAGGAGCCGCAGCAGTGTGTCCAATTCAAACCAGCAGCAAAGAGCCTGACATTTTCCCATCCATCTATGAGGAAAGCCATCTCACAGAACATGGACATAGGCAACTTGCTCTCCCACACCAAGGGATGGGAATCTCTCCTACCTATAGTCATCCCTGCACTCCTGACTTTACTCCAGGACCCAGGGTCCAACTAATGGCAGAGCCCCTCTTGGTTCCTTCAAACAAGAAAAGCAATACCTACGGACTGGTGTACACTTCCATCCTTGGTTATAACAGGAATGTTATCAAGCTGTCAGAACAGGATGAAGTGCTCCCAGTGGATATCCATCAGGGAGGGTTAGGGACACTCGTGGCAGCCTGTCTAGCAGCCTGGGCTCTCTGAAAGTCCCTAACTTCCTGAGGGGTACGCAAATACTGTTCTATTTCACTATCAGAAATGTTCTCATCTCCAGTGACAGTGGAGACAGGGGGTACAGGGCAGATCCGCTTCGGGGACTTCAACATGCAGGGTGGCAAGAGAAGGGCAGGACTGGCCGGCCGCTTCCCCTGGGGTAAACCTAAGGAATTATTTCCCACCTCCCCTTCTCCTTGCCCCTGTCCCCACCCCGGTGGCTCCTTCTCTCGGGTCTCCACTTCTGCTGTCCCATCCCGAAAGGCAGAGCGGACCAGTGACTGGCGGTGCTGGAGAAGGTCACCGATGTGCTTCACCACAGACCGTTTGTCAAGTCTCAGAACTCGTAACCAGGCCAGCTGCTCAGCCATCCGCAGCAGCACAGCCAGCAGCTCCTGCAGGCGGGAGGACGCCGGGTAGGGCAGGTCCACATTTGCCAATTTACAAAATCGGGCAAGGGAACATGAAAGCCGATCTGCAGGCTGCAGCGACTGCCAAGCCAGGAAAGTCGCAGCAGTGATGACGGGCAAGGGATGCCTCCCGGTCACCAGCCACGTCTCATTTGCCAGCTCCACCAACTGCATTGTTCGAGACAGCATCTTCTCTTTGTCTTCCACGTATTTGGCTGGCACAGAAGGTGAAGCTTGGAACAGTTTGAAGCTGAAATAACCAAAATGAGGGTTGGATCTTAATGATATAGGGGCTGCTCTCCCACAGTGAGGAAAGACAGCCCACTCAAGATGGGGAAGCTATTCTGCCCTCAGGAATACTCAAGCTCACTGGGCAGCAAGTTAATAAAGGTAGTGAGAGAAAACAGGGCGTCTTCCGCTTGTTAGGGGAAGGTGGAGGGATGGAGGAGAGCACGAACATTTATTGGGCGCCTCCCAATCACCATTATTCTGAGTGCTTTACAACGTTCTCATTTAATCTACGTGCACGTGCACCATCTTATGTGCATGTATAGTTAAAAAACTTTCCCATAGTCATCCAGCCAGGCAGTAACCAAGCTTCAAATACAAGGCTATTTGACACNOV10e, CG50925-02SEQ ID NO: 2661542 aaMW at 164901.2kDProtein SequenceMRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLGNRFSTAPCPQSGLAQSFRLCPEQYSNASGWVSSEAARFLTGHNWSASDAGRPACIFPGPVSWRQVPAYNAGQDQGSKYSFYGGKVKDEYLRRSPICRSAAALHTDTSARSWSEFRLLSNNKITGLRNGSFLGLSLLEKLLMRAHFSDEDEKTACPSPAPRLWRLHSKCTLSMAGNQKNQGGSSSQEAASGLMPSRRLQRGEVEGLCTCLCTDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAHALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMPSNLMLVDEHLLWLAQREDKACSRIVGAIERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGLHPVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWRAPPPQEGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAGEELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWPACCPPASPAAPHAPPRALPAAAEDGSPVFGEGPPSLKSSPSGSSGHPLALGPCKLTNLQLAQSQVCEAGAAAGGEGEPEPAGTRGNLAHRBPNNVHHGRRAHKSRAKGHRAGEACGKNRLKALRGGAAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRAGQRRSASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKTGLWKSETTVNOV10f, 2482O8844SEQ ID NO: 2671888 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCAGGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATGGCCAGCAACCTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCCTGGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCCTCGAGNOV10f, 248208844SEQ ID NO: 268629 aaMW at 68481.1kDProtein SequenceTGSELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAQALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSLENOV10g, CG50925-03SEQ ID NO: 2691329 bpDNA SequenceORF Start: at 7ORF Stop: at 1324GGATCCCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTGGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGGGGTGATGGTGGACATGGCCAGCAACCTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCCTGGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACGTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAGCTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACGCCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGTCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCCTCGAGNOV10g, CG50925-03SEQ ID NO: 270439 aaMW at 47486.5kDProtein SequenceRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVGVMVDMASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNASRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTESVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSNOV10h, CG50925-04SEQ ID NO: 27160 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGAGAAGCTTCTCATGAGAGCACATTTTTCTGATGAAGATGAAAAGACGGCCTGTCCTTCTNOV10h, CG50925-04SEQ ID NO: 27220 aaMW at 2307.5kDProtein SequenceEKLLMRAHFSDEDEKTACPSNOV10i, CG50925-05SEQ ID NO: 27357 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACNOV10i, CG50925-05SEQ ID NO: 27419 aaMW at 2275.4kDProtein SequenceRASRRCDRAGRWEPGDYSHNOV10j, CG50925-06SEQ ID NO: 27557 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGAGCTGGTAGAGGTGATGGTGGACATGCCCAGCAACTTGATGCTGGTGGACGAGCACNOV10j, CG50925-06SEQ ID NO: 27619 aaMW at 2200.6kDProtein SequenceELVEVMVDMPSNLMLVDEHNOV10k, CG50925-07SEQ ID NO: 27766 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGGNOV10k, CG50925-07SEQ ID NO: 27822 aaMW at 2358.6kDProtein SequenceKAGNSHASLEAGEELRGSTRLRNOV10l, SNP13380040SEQ ID NO: 2797892 bpof CG50925-01,ORF Start: ATG at 1ORF Stop: TAA at 4627DNA SequenceSNP Pos: 2164SNP Change: G to AATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTGCCGTGGCTCCTGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCCATCCGCAGCTGCAAGTGCTCGGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGCCCGGCTCGGCGGAGGGTGGTGTGCAGCGGCGGGGACCTCCCGGAGCCTCCCGAGCCCGGCCTTCTGCCTAACGGCACCGTTACCCTTGGCAACCGTTTCTCCACTGCCCCTTGCCCGCAGTCGGGGCTGGCCCAGTCTTTTCGATTGTGTCCAGAACAATACAGTAACGCCTCTGGTTGGGTGAGTTCAGAGGCTGCCCGCTTCCTGACAGGACACAACTGGTCAGCCTCTGACGCTGGCCGCCCGGCCTGCATTTTCCCCGGCCCGGTCTCCTGGAGACAGGTACCAGCTTACAACGCCGGACAGGATCAGGGTAGCAAATATAGCTTCTATGGAGGAAAGGTCAAAGATGAGTATCTGCGTAGGTCCCCGATCTGTCGCTCTGCTGCCGCCCTGCACACTGACACCAGTGCCCGCAGCTGGTCTGAATTTAGGCTCTTGAGCAATAACAAGATCACGGGGCTCCGCAATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTTCTCATGAGAGCACATTTTTCTGATGAAGATGAAAAGACGGCCTGTCCTTCTCCAGCACCAAGGCTCTGGAGGCTGCATAGCAAGTGCACCCTGAGCATGGCAGGCAATCAGAAGAACCAGGGCGGCAGCAGCTCACAAGAGGCAGCATCTGGCTTGATGCCGAGTCGGCGGCTCCAGCGGGGAGAGGTTGAGGGCCTCTGCACTTGCCTCTGCACGGACCTGAGGAACAACATCATCAGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCCAACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGACTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGGCTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTACCCCAGTGCCCTGCATGCTCATGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAGGGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAGGGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGCTGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAGAGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTGTGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAGGTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAACAACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGCCTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCCCGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACCAACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCGCTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATGATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATCAAAGAGCTGGTAGAGGTGATGGTGGACATGCCCAGCAACTTGATGCTGGTGGACGAGCACCTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCATAGAGCGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAACATGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCCTTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCCCCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGGCCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACACCGGTGCCCCCAGACTGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACCTCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTCGCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGGCACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAGGCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCCCTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGGGAGGTGGGGGGCGCCGGGGCAGGGCTGCACCCCGTGGTATACCCCTGCACGGCCTTGCTGCTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCAACCACAGCTCCATCCGTGTGTCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCATGACCTCTGCTGTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATCACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTCCATAAGGAGCTCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACTCCCAGTCCTATGCTCCGGTACATACTTTCAATTCCAGCTTTGCAATTGGGGAGGGACTCCAACGCAGGCGTAGGAAACCTCCCAAGGTTCTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGCATCACAGCTGCAGTCAACATCCACAACTACCGGGACCACAGCCCCTACTGCTGGCTGGTGTGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTTTGATTCTGCTCATCACCTGGATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAACCCCAAGGCGGGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGGGGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTGGGGACGCCCGGGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGGGCGCTGGTGACCACGCACTTCCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTGTCCCAGCGCTGGCTGCCCCGGGTGGTGTGCAGCTGCTTGTACGGGGTGGCAGCCTCCGCCCTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGAGGGACGTGAGAGCCTCGTGGCGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCGGGCCCTGCCCGCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGGCCCCCCTCCCTCAAGTCCTCCCCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAGCTGGCCCAGAGTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAGCCGGCGGGCACCCGGGGAAACCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGTCGGGCGCACAAGAGCCGGGCCAAGGGACACCGCGCGGGGGAGGCCTGCGGCAAGAACCGGCTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAACTGCTGTCCAGCGAGAGCGGTAGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAGCCCGGGCGCCGGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCCGCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTCAAGGGCGGCGGCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCCAGCCTAAACGGCGCCCCCAAGGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAGGTAGCCAGCGGCGGCTGCATGAAGACCGGACTCTGGAAGAGCGAAACTACCGTCTAAGGTGGGGCGGGCGACGCGGTAGACGGGTTGGCCACGCGGCTCGTTCCCCCGCTCCTCGGGGCCCTCCAAGGTGTCTCCGTAGTCAGCAGGTTGGAGGCAGAGGAGCCGATGGCTGGAGGAAGCCCACAGGCGGATGTTCCCCACTTGCCTAGAGGGCATCCCTCTGGGGTAGCGACAGACAATCCCAGAAACACGCATAATACATTTCCGTCCAGCCCGGGGCAGTCTGACTGTCGGTGCCCTCCCAGGAACGGGGAAGGCCTCCGTCTGTGTGAAAGGGCACAGCACATCCCAGGTGCACCCTCCCCAAGTACTCCCACCCCGCCTACTGTCCATGCGGCCTCACTGGGGGCCATCAGCCTCACCAGCAAAGCAGAGATGAGAGCGTGGGAACTGTGTTCTTTCCTCCCTGCCCTCTACTGATTTCAGCCCAGCCCCTGCCTAGATCCTAGGTCCCTTTTCCTCCCGAGTTTGGCTGGCACGAGAGCTAGCCCAGCACATGAAGCAGGTGATGTTAAGTCACAAGGTGCTGCTTTTCAGATCCACTATGCAAGAGGGGAGGGTGGGGCCACGTGAAAGGCAGCTCTAGACATCAACCAGTCCTGGGGGAGGGGAGTGGGAACCGGGCACAACTAGGAACAATGCCACCATTCCCACAGGAGTGGTACTTAAACCAGACAGCAGGGTTCAGAGGTGGCACACCGGGACAAAGCTGAGGCCCTGCACCTCAACAGCTGACTGCCAGGTGCCTGTGGGTGAACTGAGGGGAGTAGAGGGAGAGGGCAGGTGGAACTGGGGCAGAATCTAGTCATGCCCTAAAGCTAGTCCTGTAAACAATGGTGCCCCAGAAAGCTGCAGGTGGTGTTTGGAGAAGCAGTTACTTTTCAGTTACAAGACCCATCTCCCTAGTCTCAGCCTTACAACACCACGGGACTAAGGAAGAGCACTTCCTTGCCTCCGTAAGGCCAGAGGAAGAACCATCCCAATCATTTGATCTCCAGCTCCACAGTAGAGAGAAACCTACAAAATGTCAAACCAGCTTCCCGACTCCCAGGAGCTCAAGCCAAGCCCAGAGGCAGTGGCTGGGGTCCCTGCAGGTCATGAGGGGCCTATGCCTTTACTCCTTTTAAACACCAGCACCCGTCTTTTCCCCAACCTAAAACCAACCACCAGCATTTCACTACAGGACCAAATGGAAACCGAGGGAACCCTGGGTCTTGGGAAGAACAACAGGAAACCAAGGTCTGACCTAGGGTTCCCTCCCAGTCTTCACATCACTCTGGCCTCATCACCAAGGTGACAGAGGACACAGGGGAGGGGGAAAACCCACACACACTCCTTGGAATGGGTCCTGTTATTTATGCTTGCTGCACAGACATATTAGAAGAAAAAAAAAAAGCTTTGTATTATTCTTCCACATATGCTGGCTGCTGTTTACACACCCTGCCAATGCCTTAGCACTGGAGAGCTTTTTGCAATATGCTGGGGAAAGGGGAGGGAGGGAATGAAAGTGCCAAAGAAAACATGTTTTTAAGAACTCGGGTTTTATACAATAGAATGTTTTCTAGCAGATGCCTCTTGTTTTAATATATTAAAATTTTGCAAAGCCCTTTGAGCTACTGCCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACACCATACACACAAACCCATCATTGCCTGTGAATGCACGTAGGGCCAGAATTCCCCAGTTCCCGCTCCTCTGAGGGTTGATACTGCTGGGAATGCCAACCACTCCACAAGCAGAGGGAAGCCCCCTCAGGCCTGCAGGAGGAGCCGCAGCAGTGTGTCCAATTCAAACCAGCAGCAAAGAGCCTGACATTTTCCCATCCATCTATGAGGAAAGCCATCTCACAGAACATGGACATAGGCAACTTGCTCTCCCACACCAAGGGATGGGAATCTCTCCTACCTATAGTCATCCCTGCACTCCTGACTTTACTCCAGGACCCAGGGTCCAACTAATGGCAGAGCCCCTCTTGGTTCCTTCAAACAAGAAAAGCAATACCTACGGACTGGTGTACACTTCCATCCTTGGTTATAACAGGAATGTTATCAAGCTGTCAGAACAGGATGAAGTGCTCCCAGTGGATATCCATCAGGGAGGGTTAGGGACACTCGTGGCAGCCTGTCTAGCAGCCTGGGCTCTCTGAAAGTCCCTAACTTCCTGAGGGGTACGCAAATACTGTTCTATTTCACTATCAGAAATGTTCTCATCTCCAGTGACAGTGGAGACAGGGGGTACAGGGCAGATCCGCTTCGGGGACTTCAACATGCAGGGTGGCAAGAGAAGGGCAGGACTGGCCGGCCGCTTCCCCTGGGGTAAACCTAAGGAATTATTTCCCACCTCCCCTTCTCCTTGCCCCTGTCCCCACCCCGGTGGCTCCTTCTCTCGGGTCTCCACTTCTGCTGTCCCATCCCGAAAGGCAGAGCGGACCAGTGACTGGCGGTGCTGGAGAAGGTCACCGATGTGCTTCACCACAGACCGTTTGTCAAGTCTCAGAACTCGTAACCAGGCCAGCTGCTCAGCCATCCGCAGCAGCACAGCCAGCAGCTCCTGCAGGCGGGAGGACGCCGGGTAGGGCAGGTCCACATTTGCCAATTTACAAAATCGGGCAAGGGAACATGAAAGCCGATCTGCAGGCTGCAGCGACTGCCAAGCCAGGAAAGTCGCAGCAGTGATGACGGGCAAGGGATGCCTCCCGGTCACCAGCCACGTCTCATTTGCCAGCTCCACCAACTGCATTGTTCGAGACAGCATCTTCTCTTTGTCTTCCACGTATTTGGCTGGCACAGAAGGTGAAGCTTGGAACAGTTTGAAGCTGAAATAACCAAAATGAGGGTTGGATCTTAATGATATAGGGGCTGCTCTCCCACAGTGAGGAAAGACAGCCCACTCAAGATGGGGAAGCTATTCTGCCCTCAGGAATACTCAAGCTCACTGGGCAGCAAGTTAATAAAGGTAGTGAGAGAAAACAGGGCGTCTTCCGCTTGTTAGGGGAAGGTGGAGGGATGGAGGAGAGCACGAACATTTATTGGGCGCCTCCCAATCACCATTATTCTGAGTGCTTTACAACGTTCTCATTTAATCTACGTGCACGTGCACCATCTTATGTGCATGTATAGTTAAAAAACTTTCCCATAGTCATCCAGCCAGGCAGTAACCAAGCTTCAAATACAAGGCTATTTGACACCAACAGCCTCTACTTTCAACGTTATTTATCAGNOV10l, SNP13380040SEQ ID NO: 2801542 aaMW at 164933.2kDof CG50925-01,SNP Pos: 722SNP Change: Val to MetProtein SequenceMRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLGNRFSTAPCPQSGLAQSFRLCPEQYSNASGWVSSEAARFLTGHNWSASDAGRPACIFPGPVSWRQVPAYNAGQDQGSKYSFYGGKVKDEYLRRSPICRSAAALHTDTSARSWSEFRLLSNNKITGLRNGSFLGLSLLEKLLMRAHFSDEDEKTACPSPAPRLWRLHSKCTLSMAGNQKNQGGSSSQEAASGLMPSRRLQRGEVEGLCTCLCTDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAHALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMPSNLMLVDEHLLWLAQREDKACSRIVGAIERIGGAALSPHAQHISVNARNMALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGLHPVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWRAPPPQEGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAGEELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPAAPHAPPRALPAAAEDGSPVFGEGPPSLKSSPSGSSGHPLALGPCKLTNLQLAQSQVCEAGAAAGGEGEPEPAGTRGNLAHRHPNNVHHGRRAHKSPAKGHRAGEACGKNRLKALRGGAAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRAGQRRSASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKTGLWKSETTV


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 10B.

TABLE 10BComparison of the NOV10 protein sequences.NOV10a------------------------------------------------------------NOV10bMRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bCSGGDLPEPPEPGLLPNGTVTLDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bLSEHTLCAYPSALHAHALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bGGAPGTRASRRCDRAGRWESGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLNLVDEHLLWLAQREDKANOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bCSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTNOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPANOV10c------------------------------------------------------------NOV10d--------------------------------------------TGSAPGCPLSIRSCKCNOV10e------------------------------------------MRGAPARLLLPLLPWLLLNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLNOV10c------------------------------------------------------------NOV10dSGERAKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLLLSNNKITGLRNGSFLGNOV10eLLAPEARGAPGCPLSIRSCKCSGERPKGLSGGVPGPARRRVVCSGGDLPEPPEPGLLPNGNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a------------------------------------------------------------NOV10bTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVNOV10c------------------------------------------------------------NOV10dLSLLEKLDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNNOV10eTVTLGNRFSTAPCPQSGLAQSFRLCPEQYSNASGWVSSEAARFLTGHNWSASDAGRPACINOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10a-----------MRGAPARLLLPLLPWLLLLLAPEARGAPGCPLSIRSCKCSGERPKGLSGNOV10bLMELSAFPREVGGAGAGLHPVVYPCTALLLLCLFATIITYILNNSSIRVSRKGWHMLLNLNOV10c------------------------------------------------------------NOV10dIFSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHANOV10eFPGPVSWRQVPAYNAGQDQGSKYSFYGGKVKDEYLRRSPICRSAAALHTDTSARSWSEFRNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aGVPGPARRRVVCSGGDLPEPPEPGLLPNGTVTLGNRFSTAPCPQSGLAQSFRLCPEQYSNNOV10bCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWRAPPPNOV10c------------------------------------------------------------NOV10dQALGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHDEANOV10eLLSNNKITGLRNGSFLGLSLLEKLLMRAHFSDEDEKTACPSPAPRLWRLHSKCTLSMAGNNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aASGWVSSEAARFLTGHNWSASDAGRPACIFPGPVSWRQVPAYNAGQDQGSKYSFYGGKVKNOV10bQEGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNOV10c----------------------------------------------------TGSELKRLNOV10dPVEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLNOV10eQKNQGGSSSQEAASGLMPSRRLQRGEVEGLCTCLCTDLRNNIISTVQPGAFLGLGELKRLNOV10f----------------------------------------------------TGSELKRLNOV10g-------------------------------RLPFQCSASYLGNDTRIRWYHNHAPVEGDNOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aDEYLRRSPICRSAAALHTDTSARSWSEFRLLSNNKITGLRNGSFLGLSLLEKLLMRAHFSNOV10bNYRDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLENOV10cDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHNOV10dETSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRCDRANOV10eDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHNOV10fDLSNNRIGCLTSETFQGLPRLLRLNISGNIFSSLQPGVFDELPALKVVDLGTEFLTCDCHNOV10gEQAGILLAESLIHDCTFITRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTHASRRCDRANOV10h--------------------------------------------------EKLLMRAHFSNOV10i---------------------------------------------------RASRRCDRANOV10j--------------------------------------------------ELVEVMVDMPNOV10k--------------------------------------------------KAGNSRASLENOV10aDEDEKTACPSPAPRLWRLHSKCTLSMAGNQKNQGGSSSQEAASGLMPSRRLQRGEVEGLCNOV10bAGEELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLNOV10cLRWLLPWAQNRSLQLSEHTLCAYPSALHAQALGSLQEAQLCCELE---------------NOV10dGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDNMDVVYVNOV10eLRWLLPWAQNRSLQLSEHTLCAYPSALHAHALGSLQEAQLCCEGALELHTHHLIPSLRQVNOV10fLRWLLPWAQNRSLQLSEHTLCAYPSALHAQALGSLQEAQLCCEGALELHTHHLIPSLRQVNOV10gGRWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVNOV10hDEDEKTACPS--------------------------------------------------NOV10iGRWEPGDYSH--------------------------------------------------NOV10jSNLMLVDEH---------------------------------------------------NOV10kAGEELRGSTRLR------------------------------------------------NOV10aTCLCTDLRNNIISTVQPGAFLGLGELKRLDLSNNRIGCLTSETFQGLPRLLRLNISGNIFNOV10bYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARPPDVRASWRACCPPASNOV10c------------------------------------------------------------NOV10dAQMIQKFLGYVDQIKELVEVMVDMASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAANOV10eVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHNOV10fVFQGDRLPFQCSASYLGNDTRIRWYHNRAPVEGDEQAGILLAESLIHDCTFITSELTLSHNOV10gAQMIQKFLGYVDQIKELVGVMVDMASNLMLVDEHLLWLAQREDKACSRIVGALERIGGAANOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aSSLQPGVFDELPALKVVDLGTEFLTCDCHLRWLLPWAQNRSLQLSEHTLCAYPSALHAHANOV10bPAAPHAPPRALPAAAEDGSPVFGEGPPSLKSSPSGSSGHPLALGPCKLTNLQLAQSQVCENOV10c------------------------------------------------------------NOV10dLSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPNOV10eIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYNOV10fIGVWASGEWECTVSMAQGNASKKVEIVVLETSASYCPAERVANNRGDFRWPRTLAGITAYNOV10gLSPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPNOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aLGSLQEAQLCCEGALELHTHHLIPSLRQVVFQGDRLPFQCSASYLGNDTRIRWYHNEAPVNOV10bAGAAAGGEGEPEPAGTRGNLAHRHPNNVHHGRRAHKSRAKGHRAGEACGKNRLKALRGGANOV10c------------------------------------------------------------NOV10dPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLNOV10eQSCLQYPFTSVPLGGGAPGTRASRRCDEAGRWEPGDYSHCLYTNDITRVLYTFVLMPINANOV10fQSCLQYPFTSVPLGGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVLYTFVLMPINANOV10gPADQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLNOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aEGDEQAGILLAESLIHDCTFITSELTLSHIGVWASGEWECTVSMAQGNASKKVEIVVLETNOV10bAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRNOV10c------------------------------------------------------------NOV10dVFRTGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGANOV10eSNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMPSNLMLVNOV10fSNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLVNOV10gVFRNGRLFHSHSNASRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTESVAVSLRHWAEGANOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aSASYCPAERVANNRGDFRWPRTLAGITAYQSCLQYPFTSVPLGGGAPGTRASRRcDRAGRNOV10bAGQRRSASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKNOV10c------------------------------------------------------------NOV10dEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGNOV10eDEHLLWLAQREDKACSRIVGAIERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTNOV10fDEHLLWLAQREDKACSRIVGALERIGGAALSPHAQHISVNARNVALEAYLIKPHSYVGLTNOV10gEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELS----------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aWEPGDYSHCLYTNDITRVLYTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQNOV10bTGLWKSETTV--------------------------------------------------NOV10c------------------------------------------------------------NOV10dAGLLEG------------------------------------------------------NOV10eCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALANOV10fCTAFQRREGGVPGTRPGSPGQNPPPEPEPPADQQLRFRCTTGRPNVSLSSFHIKNSVALANOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aMIQKFLGYVDQIKELVEVMVDMPSNLMLVDEHLLWLAQREDKACSRIVGAIERIGGAALSNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eSIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPNOV10fSIQLPPSLFSSLPAALAPPVPPDCTLQLLVFRNGRLFHSHSNTSRPGAAGPGKRRGVATPNOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aPHAQHISVNARNVALEAYLIKPHSYVGLTCTAFQRREGGVPGTRPGSPGQNPPPEPEPPANOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNNOV10fVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPVAAWWSQEGPGEAGGWTSEGCQLRSSQPNNOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aDQQLRFRCTTGRPNVSLSSFHIKNSVALASIQLPPSLFSSLPAALAPPVPPDCTLQLLVFNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eVSALHCQHLGNVAVLMELSAFPREVGGAGAGLHPVVYPCTALLLLCLFATIITYILNHSSNOV10fVSALHCQHLGNVAVLMELSLE---------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aRNGRLFHSHSNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGAEPNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLTNYQMVCQAVGITLHYSSLSTLLWMGVKANOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aVAAWWSQEGPGEAGGWTSEGCQLRSSQPNVSALHCQHLGNVAVLMELSAFPREVGGAGAGNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eRVLHKELTWHAPPPQEGDPALPTPSPMLRYILSIPALQLGRDSNAGVGNLPRFYLIAGGINOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aLHPVVYPCTALLLLCLFATIITYILNHSSIRVSRKGWHMLLNLCFHIAMTSAVFAGGITLNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10ePLIICGITAAVNIHNYRDHSPYCWLVWRPSLGAFYIPVALILLITWIYFLCAGLRLRGPLNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aTNYQMVCQAVGITLHYSSLSTLLWMGVKARVLHKELTWRAPPPQEGDPALPTPSPMLRYINOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eAQNPKAGNSRASLEAGEELRGSTRLRGSGPLLSDSGSLLATGSARVGTPGPPEDGDSLYSNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aLSIPALQLGRDSNAGVGNLPRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWLVWRPSLNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10ePGVQLGALVTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASALGLFVFTHHCARRRNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aGAFYIPVALILLITWIYFLCAGLRLRGPLAQNPKAGNSRASLEAGEELRGSTRLRGSGPLNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NQV10eDVEASWRACCPPASPAAPHAPPRAIPAAAEDGSPVFGEGPPSLKSSPSGSSGHPLALGPCNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aLSDSGSLLATGSARVGTPGPPEDGDSLYSPGVQLGALVTTHFLYLAMWACGALAVSQRWLNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eKLTNLQLAQSQVCEAGAAAGGEGEPEPAGTRGNLAHRHPNNVHHGRPAHKSRAKGHRAGENOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aPRVVCSCLYGVAASALGLFVFTHHCARRRDVRASWRACCPPASPAAPHAPPRALPAAAEDNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eACGKNRLKALRGGAAGALELLSSESGSLHNSPTDSYLGSSRNSPGAGLQLEGEPMLTPSENOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aGSPVFGEGPPSLKSSPSGSSGHPLALGPCKLTNLQLAQSQVCEAGAAAGGEGEPEPAGTRNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eGSDTSAAPLSEAGRAGQRRSASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVNOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aGNLAHRHPNNVHHGRRAHKSRAKGHRAGEACGKNRLKALRGGAAGALELLSSESGSLHNSNOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10eTLMGAEVASGGCMKTGLWKSETTV------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRAGQRRSASRDSLKGGGANOV10b------------------------------------------------------------NOV10c------------------------------------------------------------NOV10d------------------------------------------------------------NOV10e------------------------------------------------------------NOV10f------------------------------------------------------------NOV10g------------------------------------------------------------NOV10h------------------------------------------------------------NOV10i------------------------------------------------------------NOV10j------------------------------------------------------------NOV10k------------------------------------------------------------NOV10aLEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASGGCMKTGLWKSETTVNOV10b-----------------------------------------------------NOV10c-----------------------------------------------------NOV10d-----------------------------------------------------NOV10e-----------------------------------------------------NOV10f-----------------------------------------------------NOV10g-----------------------------------------------------NOV10h-----------------------------------------------------NOV10i-----------------------------------------------------NOV10j-----------------------------------------------------NOV10k-----------------------------------------------------NOV10a(SEQ ID NO: 258)NOV10b(SEQ ID NO: 260)NOV10c(SEQ ID NO: 262)NOV10d(SEQ ID NO: 264)NOV10e(SEQ ID NO: 266)NOV10f(SEQ ID NO: 268)NOV10g(SEQ ID NO: 270)NOV10h(SEQ ID NO: 272)NOV10i(SEQ ID NO: 274)NOV10j(SEQ ID NO: 276)NOV10k(SEQ ID NO: 278)


Further analysis of the NOV10a protein yielded the following properties shown in Table 10C.

TABLE 10CProtein Sequence Properties NOV10aSignalP analysis:Cleavage site between residues 27 and 28PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 7; pos. chg 2; neg. chg 0H-region:length 15; peak value 9.66PSG score:5.26GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 5.33possible cleavage site: between 26 and 27>>> Seems to have a cleavable signal peptide (1 to 26)ALOM:Klein et al's method for TM region allocationInit position for calculation: 27Tentative number of TMS(s) for the threshold 0.5: 5INTEGRALLikelihood =Transmembrane 958-974−7.91INTEGRALLikelihood =Transmembrane  988-1004−1.22INTEGRALLikelihood =Transmembrane 1093-1109−6.26INTEGRALLikelihood =Transmembrane 1136-1152−8.17INTEGRALLikelihood =Transmembrane 1252-1268−3.50PERIPHERALLikelihood = 1.11 (at 1223)ALOM score: −8.17 (number of TMSs: 5)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 13Charge difference: −2.0 C(1.0)-N(3.0)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):12.41Hyd Moment(95):9.21G content: 1D/E content:1S/T content: 0Score: −2.41Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 68 RRV|VCNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PGPARRR (3) at 52pat7: PARRRVV (5) at 54pat7: PGKRRGV (5) at 849bipartite: nonecontent of basic residues: 9.1%NLS Score: 0.65KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: RGAPnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):43.5%: endoplasmic reticulum34.8%: nuclear13.0%: mitochondrial 4.3%: cytoplasmic 4.3%: peroxisomal>> prediction for CG50925-01 is end (k = 23)


A search of the NOV10a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 10D.

TABLE 10DGeneseq Results for NOV10aNOV10aResidues/Identities/GeneseqProtein/Organism/LengthMatchSimilarities for theExpectIdentifier[Patent #, Date]ResiduesMatched RegionValueABU54447Human tumour endothelial marker263 . . . 15421231/1292 (95%)0.0TEM 9 - Homo sapiens, 1331 aa. 63 . . . 13311236/1292 (95%)[WO200283874-A2, 24-OCT-2002]ABU54432Human tumour endothelial marker263 . . . 15421231/1292 (95%)0.0TEM 5 - Homo sapiens, 1331 aa. 63 . . . 13311236/1292 (95%)[WO200283874-A2, 24-OCT-2002]AAU74824Human REPTR 7 protein - Homo263 . . . 15421231/1292 (95%)0.0sapiens, 1331 aa. [WO200198354-A2, 63 . . . 13311236/1292 (95%)27-DEC-2001]ABB90740Human Tumour Endothelial Marker263 . . . 15421231/1292 (95%)0.0polypeptide SEQ ID NO 212 - Homo 63 . . . 13311236/1292 (95%)sapiens, 1331 aa. [WO200210217-A2,07-FEB-2002]ABB90725Human Tumour Endothelial Marker263 . . . 15421231/1292 (95%)0.0polypeptide SEQ ID NO 188 - Homo 63 . . . 13311236/1292 (95%)sapiens, 1331 aa. [WO200210217-A2,07-FEB-2002]


In a BLAST search of public sequence databases, the NOV10a protein was found to have homology to the proteins shown in the BLASTP data in Table 10E.

TABLE 10EPublic BLASTP Results for NOV10aNOV10aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96PE1Tumor endothelial marker 5263 . . . 15421231/1292 (95%)0.0precursor - Homo sapiens 63 . . . 13311236/1292 (95%)(Human), 1331 aa.Q8TEM3FLJ00170 protein - Homo sapiens263 . . . 15421230/1292 (95%)0.0(Human), 1310 aa (fragment). 42 . . . 13101235/1292 (95%)Q91ZV8Tumor endothelial marker 5280 . . . 15421083/1266 (85%)0.0precursor - Mus musculus 92 . . . 13291140/1266 (89%)(Mouse), 1329 aa.Q9P1Z7KIAA1531 protein - Homo sapiens923 . . . 1542 587/620 (94%)0.0(Human), 1206 aa (fragment).615 . . . 1206 589/620 (94%)Q8N3R1Hypothetical protein - Homo1031 . . . 1542  489/512 (95%)0.0sapiens (Human), 489 aa 1 . . . 489 489/512 (95%)(fragment).


PFam analysis predicts that the NOV10a protein contains the domains shown in the Table 10F.

TABLE 10FDomain Analysis of NOV10aIdentities/SimilaritiesPfamNOV10afor theExpectDomainMatch RegionMatched RegionValueLRR314 . . . 33710/25 (40%)0.008620/25 (80%)LRR338 . . . 36110/25 (40%)0.1520/25 (80%)LRRCT371 . . . 42119/54 (35%)1.3e-0840/54 (74%)HRM531 . . . 60024/92 (26%)0.008848/92 (52%)GPS887 . . . 93915/55 (27%)0.004134/55 (62%)7tm_2 950 . . . 128065/359 (18%) 0.0032207/359 (58%) 


Example 11

The NOV11 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 11A.

TABLE 11ANOV11 Sequence AnalysisNOV11a, CG51027-06SEQ ID NO: 2811271 bpDNA SequenceORF Start: ATG at 48ORF Stop: TAG at 1056GCGCAGTGTGCTGGAATTCGCCCTTCCTCCGTGCCGCCTCTGCCGCGATGCCCCCCCCTGCGCCCGGCGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGATGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTATAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGATGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTTGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGTGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCGCAACCCAGTGGAGCTCGCCTCCCCCTGCAGGGGGCCTCAGGCCAAGAGTGAGAGAAAAGGGGGAGCAAGAGCCCTGGGTCTCGTGGGGGCAGAACGGCTCTCGGCCACCAAGGAAGAAGAGAAGAGGAGAAAGAGGAGGAGGCAGAGGAAGAAAGATCTTTAGAGAACCCATCACTGTGAGGGATAACGCAAAATTATGCATCTTTCTACNOV11a, CG51027-06SEQ ID NO: 282336 aaMW at 36748.4kDProtein SequenceMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANVSARHYGNYTCCAANRLGASSASMRLLCPGSLENSAPRPPGLIJALLSALGWLWWRMNOV11b, 278391205SEQ ID NO: 2831033 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTCCCACCATGCCCCCCCCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGCTCGAGGGCNOV11b, 278391205SEQ ID NO: 284344 aaMW at 37634.3kDProtein SequenceTKLPTMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSPRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMLEGNOV11c, 278391050SEQ ID NO: 285976 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGCTCGAGGGCNOV11c, 278391050SEQ ID NO: 286325 aaMW at 35781.1kDProtein SequenceTKLLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMLEGNOV11d, 277582210SEQ ID NO: 2871760 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCCTCGAGGGCNOV11d, 277582210SEQ ID NO: 288253 aaMW at 28074.3kDProtein SequenceTKLGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRALEGNOV11e, 277582173SEQ ID NO: 289946 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGCTCGAGGGCNOV11e, 277582173SEQ ID NO: 290315 aaMW at 34843.0kDProtein SequenceTKLLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMELLRPGSLENSAPRPPGLLALLSALGWLWWRMLEGNov11f, CG51027-09SEQ ID NO: 291976 bpDNA SequenceORF Start: at 11ORF Stop: at 968CACCAAGCTTCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGCTCGAGGGCNOV11f, CG51027-09SEQ ID NO: 292319 aaMW at 35139.3kDProtein SequenceLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDENVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMNOV11g, 316763704SEQ ID NO: 293976 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCTCGCGACTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGGTCGACGGCNOV11g, 316763704SEQ ID NO: 294325 aaMW at 35755.0kDProtein SequenceTSRLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFAINSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMVDGNOV11h, CG51027-01SEQ ID NO: 2951018 bpDNA SequenceORF Start: at 1ORF Stop: TAG at 958CTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCTTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGACCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAATCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCGCAACCCAGTCCAGCTCACCTCCCCCTGCAGGGGGCCTCAAACCAAGAGTGAGAGANOV11h, CG51027-01SEQ ID NO: 296319 aaMW at 35169.4kDProtein SequenceLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRTALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMNOV11i, CG51027-02SEQ ID NO: 297926 bpDNA SequenceORF Start: at 1ORF Stop: TAG at 922TCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCNOV11i, CG51027-02SEQ ID NO: 298307 aaMW at 33974.9kDProtein SequenceSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMNOV11j, CG51027-03SEQ ID NO: 2991171 bpDNA SequenceORF Start: at 1ORF Stop: TAG at 958CTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGTGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGATGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTATAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGATGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCGCAACCCAGTGGAGCTCACCTCCCCCTGCAGGGGGCCTCAGGCCAAGAGTGAGAGAAACGGGGGAGCAAGAGCCGTGGGTCTCGTGGGGGCAGAAGAGCTCTCGGCCACCAAGGAAGAAGAGAGAGGAGAAGAGGAGGAGGCAGAGGAAGAAAGATCTTCAGAGAACCCATCACTGTGAGGGATAACGCAAAATTATGCATCTTTCTACNOV11j, CG51027-03SEQ ID NO: 300319 aaMW at 35229.6kDProtein SequenceLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVWLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANVSARHYGNYTCRAACRLGASSASMRLLRPGSLENSAPRPPGLLALLSALSWLWWRMNOV11k, CG51027-04SEQ ID NO: 301927 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGNOV11k, CG51 027-04SEQ ID NO: 302309 aaMW at 33881.8kDProtein SequenceLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALNOV11l, CG51027-05SEQ ID NO: 3031169 bpDNA SequenceORF Start: ATG at 71ORF Stop: TAG at 1079CTCCAACAAGCATAGCGCGCCCCGGACCGGCCCCCCCTTTCCCCTCCCCCTCCGTGCCGCCTCTGCCGCGATGCCCCCCGCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGGGGGCTGCTCTCCCAGAGGCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATGGACGAGCATGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTACGCCGGCAACGACCGCAGGACCAGGGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCGTCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCGTTGTGAACATCTCGTCGCCTGTGATGGTGAATGAGGGAGGTAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCTGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGT9ACCAGCGCCCGCACCGCGCTGGGGCCGGGCCGCCTACTGCGCTGCGAAGCCATGGCGGTTTCCCCCGCGGATTTCCAGTGGTATAAGGATGACAGACTACTGAGCAGCGGCACGGCCGAGGGCCTGAAGGTGCAGATGGAGCGCACTCGCTCGATGCTTCTCTTTGCCAACATGAGCGCCCGGCATTACGGCAACTATACGTGTTGCGCCGCCAACCGGCTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGTGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCCCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCGCAACCCAGTGGAGCTCGCCTCCCCCTGCAGGGGGCCTCAGGCCAAGAGTGAGAGAAAAGGGGGAGCAAGAGCCCTGGGTCTCGTGNOV11l, CG51027-05SEQ ID NO: 304336 aaMW at 36823.6kDProtein SequenceMPPAAPGARLRLLAAAALAGLAVISRGLLSQRLEFNSPADNYTVCEGDNATLSCFMDEHVTRVAWLNRSNILYAGNDRRTRDPRVRLLINTPEEFSILVTEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARVVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDILRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGPGRLLRCEAMAVSPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANMSARHYGNYTCCAANRLGAS SASMRLLCPGSLENSAPRPPGPLALLSALGWLWWRMNOV11m, CG51027-07SEQ ID NO: 305946 bpDNA SequenceORF Start: at 11ORF Stop: at 938CACCAAGCTTCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGCTCGAGGGCNOV11m, CG51027-07SEQ ID NO: 306309 aaMW at 34201.2kDProtein SequenceLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSMILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMNOV11n, CG51027-08SEQ ID NO: 3071033 bpDNA SequenceORF Start: ATG at 17ORF Stop: at 1025CACCAAGCTTCCCACCATGCCCCCCCCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGCTCGAGGGCNOV11n, CG51027-08SEQ ID NO: 308336 aaMW at 36794.3kDProtein SequenceMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANPLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWPMNOV11o, CG51027-10SEQ ID NO: 3091035 bpDNA SequenceORF Start: ATG at 16ORF Stop: TAG at 1024CACCGCGGCCGCACCATGCCCCCCCCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGTCGACGGCNOV11o, CG51027-10SEQ ID NO: 310336 aaMW at 36794.3kDProtein SequenceMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPALFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMNOV11p, CG51027-11SEQ ID NO: 311976 bpDNA SequenceORF Start: at 11ORF Stop: at 971CACCTCGCGACTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGGTCGACGGCNOV11p, CG51027-11SEQ ID NO: 312320 aaMW at 35238.5kDProtein SequenceLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMVNOV11q, CG51027-12SEQ ID NO: 3131030 bpDNA SequenceORF Start: ATG at 14ORF Stop: at 1022CACCTCGCGAACCATGCCCCCCCCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGACGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTACAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGACGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTCGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGCGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGGTCGACGGCNOV11q, CG51027-12SEQ ID NO: 314336 aaMW at 36794.3kDProtein SequenceMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANRLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMNOV11r, SNP13376340SEQ ID NO: 3151271 bpof CG51027-06,ORF Start: ATG at 48ORF Stop: TAG at 1056DNA SequenceSNP Pos: 888SNP Change: G to AGCGCAGTGTGCTGGAATTCGCCCTTCCTCCGTGCCGCCTCTGCCGCGATGCCCCCCCCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGATGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTATAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGATGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACATGAGCGCCCGGCATTACGGCAACTATACGTGTTGCGCCGCCAACCGACTGGGAGCGTCCAGCGCCTCCATGCGGCTCCTGTGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCGCAACCCAGTGGAGCTCGCCTCCCCCTGCAGGGGGCCTCAGGCCAAGAGTGAGAGAAAAGGGGGAGCAAGAGCCCTGGGTCTCGTGGGGGCAGAAGGGCTCTCGGCCACCAAGGAAGAAGAGAAGAGGAGAAAGAGGAGGAGGCAGAGGAAGAAAGATCTTTAGAGAACCCATCACTGTGAGGGATAACGCAAAATTATGCATCTTTCTACNOV11r, SNP13376340SEQ ID NO: 316336 aaMW at 36780.5kDof CG51027-06,SNP Pos: 281SNP Change:Protein SequenceVal to MetMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARTVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANMSARHYGNYTCCAANPLGASSASMRLLCPGSLENSAPRPPGLLALLSALGWLWWRMNOV11s, SNP13376341SEQ ID NO: 3171271 bpof CG51027-06,ORF Start: ATG at 48ORF Stop: TAG at 1056DNA SequenceSNP Pos: 941SNP Change: A to GGCGCAGTGTGCTGGAATTCGCCCTTCCTCCGTGCCGCCTCTGCCGCGATGCCCCCCCCTGCGCCCGGGGCCCGGCTCCGGCTTCTCGCCGCCGCCGCCCTGGCCGGCTTGGCCGTCATCAGCCGAGGGCTGCTCTCCCAGAGCCTGGAGTTCAACTCTCCTGCCGACAACTACACAGTGTGTGAAGGTGACAACGCCACCCTCAGCTGCTTCATCGACGAGCACGTGACCCGCGTGGCCTGGCTGAACCGCTCCAACATCCTGTATGCCGGCAATGACCGCTGGACCAGCGACCCGCGGGTGCGGCTGCTCATCAACACCCCCGAGGAGTTCTCCATCCTCATCACCGAGGTGGGGCTCGGCGACGAGGGCCTCTACACCTGCTCCTTCCAGACCCGCCACCAGCCGTACACCACTCAGGTCTACCTCATTGTCCACGTCCCTGCCCGCATTGTGAACATCTCGTCGCCTGTGATGGTGAATGAGGGGGGCAATGTGAACCTGCTTTGCCTGGCCGTGGGGCGGCCAGAGCCCACGGTCACCTGGAGACAGCTCCGAGACGGCTTCACCTCGGAGGGAGAGATCCTGGAGATCTCTGACATCCAGCGGGGCCAGGCCGGGGAGTATGAGTGCGTGACTCACAACGGGGTTAACTCGGCGCCCGACAGCCGCCGCGTGCTGGTCACAGTCAACTATCCTCCGACCATCACGGACGTGACCAGCGCCCGCACCGCGCTGGGCCGGGCCGCCCTCCTGCGCTGCGAAGCCATGGCGGTTCCCCCCGCGGATTTCCAGTGGTATAAGGATGACAGACTGCTGAGCAGCGGCACGGCCGAAGGCCTGAAGGTGCAGATGGAGCGCACCCGCTCGATGCTTCTCTTTGCCAACGTGAGCGCCCGGCATTACGGCAACTATACGTGTTGCGCCGCCAACCGACTGGGGGCGTCCAGCGCCTCCATGCGGCTCCTGTGCCCAGGATCCCTGGAGAACTCAGCCCCGAGGCCCCCAGGGCTCCTGGCCCTCCTCTCCGCCCTGGGCTGGCTGTGGTGGAGAATGTAGGCGCAACCCAGTGGAGCTCGCCTCCCCCTGCAGGGGGCCTCAGGCCAAGAGTGAGAGAAAAGGGGGAGCAAGAGCCCTGGGTCTCGTGGGGGCAGAAGGGCTCTCGGCCACCAAGGAAGAAGAGAAGAGGAGAAAGAGGAGGAGGCAGAGGAAGAAAGATCTTTAGAGAACCCATCACTGTGAGGGATAACGCAAAATTATGCATCTTTCTACNOV11s, SNP13376341SEQ ID NO: 318336 aaMW at 36748.4kDof CG51027-06,SNP Pos: 298SNP Change:Protein SequenceGly to GlyMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFIDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQTRHQPYTTQVYLIVHVPARIVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSEGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALLRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFAIVSARHYGNYTCCAANPLGASSASMRLLCPGSLENSAPRPPGLEALLSALGWLWWRM


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 11B.

TABLE 11BComparison of the NOV11 protein sequences.NOV11a -----MPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11b TKLPTMPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11c -------------------TKLLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11d ------------------------------------------------TKLGDNATLSCFNOV11e -----------------------------TKLLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11f ----------------------LAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11g -------------------TSRLAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11h ----------------------LAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11i ----------------------------------SQSLEFNSPADNYTVCEGDNATLSCFNOV11j ----------------------LAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11k -------------------------LAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11l -----MPPAAPGARLRLLAAAALAGLAVISRGLLSQRLEFNSPADNYTVCEGDNATLSCFNOV11m --------------------------------LLSQSLEFNSPADNYTVCEGDNATLSCFNOV11n -----MPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11o -----MPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11p ----------------------LAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11q -----MPPPAPGARLRLLAAAALAGLAVISRGLLSQSLEFNSPADNYTVCEGDNATLSCFNOV11a IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11b IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11c IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11d IDEHVTRVAWLNRSNTLYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11e IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11f IDEHVTRVAWLNRSNTLYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11g IDEBVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11h IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11i IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11j IDEHVTRVAWLNRSNILYAGNDRWTSDPRVWLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11k IDENVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11l MDEHVTRVAWLNRSNILYAGNDRRTRDPRVRLLINTPEEFSILVTEVGLGDEGLYTCSFQNOV11m IDENVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11n IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11o IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11p IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11q IDEHVTRVAWLNRSNILYAGNDRWTSDPRVRLLINTPEEFSILITEVGLGDEGLYTCSFQNOV11a TRHQPYTTQVYLIVHVPARIVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11b TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11c TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11d TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11e TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11f TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11g TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11h TRHQPYTTQVYLIVHVPARIVNISSPVRTNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11i TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11j TRHQPYTTQVYLIVHVPARIVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11k TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11l TRHQPYTTQVYLIVHVPARVVNISSPVMVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11m TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11n TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCIAVGRPEPTVTWRQLRDGFTSNOV11o TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11p TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11q TRHQPYTTQVYLIVHVPARIVNISSPVTVNEGGNVNLLCLAVGRPEPTVTWRQLRDGFTSNOV11a EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11b EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11c EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11d EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11e EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11f EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11g EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11h EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRTALNOV11i EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11j EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11k EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11l EGEILEISDILRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGPGRLNOV11m EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11n EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11o EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11p EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11q EGEILEISDIQRGQAGEYECVTHNGVNSAPDSRRVLVTVNYPPTITDVTSARTALGRAALNOV11a LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANVSARHYGNYTCCAANNOV11b LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11c LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11d LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRALENOV11e LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11f LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11g LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11h LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11i LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11j LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANVSARHYGNYTCRAANNOV11k LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11l LRCEAMAVSPADFQWYKDDRLLSSGTAEGLKVQMERTRSMLLFANMSARHYGNYTCCAANNOV11m LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11n LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCPAANNOV11o LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11p LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11q LRCEAMAVPPADFQWYKDDRLLSSGTAEGLKVQTERTRSMLLFANVSARHYGNYTCRAANNOV11a RLGASSASMRLLCPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11b RLGASSASMPLLRPGSLENSAPRPPGLLALLSALGWLWWRMLEGNOV11c RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMLEGNOV11d G-------------------------------------------NOV11e RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMLEGNOV11f RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWPM---NOV11g RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRMVDGNOV11h RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11i RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11j RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11k RLGASSASMRLLRPGSLENSAPRPPGLLALLSAL----------NOV11l RLGASSASMRLLCPGSLENSAPRPPGPLALLSALGWLWWRM---NOV11m RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11n RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11o RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11p RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWPMV--NOV11q RLGASSASMRLLRPGSLENSAPRPPGLLALLSALGWLWWRM---NOV11a (SEQ ID NO: 282)NOV11b (SEQ ID NO: 284)NOV11c (SEQ ID NO: 286)NOV11d (SEQ ID NO: 288)NOV11e (SEQ ID NO: 290)NOV11f (SEQ ID NO: 292)NOV11g (SEQ ID NO: 294)NOV11h (SEQ ID NO: 296)NOV11i (SEQ ID NO: 298)NOV11j (SEQ ID NO: 300)NOV11k (SEQ ID NO: 302)NOV11l (SEQ ID NO: 304)NOV11m (SEQ ID NO: 306)NOV11n (SEQ ID NO: 308)NOV11o (SEQ ID NO: 310)NOV11p (SEQ ID NO: 312)NOV11q (SEQ ID NO: 314)


Further analysis of the NOV11a protein yielded the following properties shown in Table 11C.

TABLE 11CProtein Sequence Properties NOV11aSignalP analysis:Cleavage site between residues 28 and 29PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 11; pos. chg 2; neg. chg 0H-region:length 14; peak value 8.95PSG score:4.55GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −2.40possible cleavage site: between 22 and 23>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 12-28−2.92PERIPHERALLikelihood = 2.49 (at 128)ALOM score: −2.92 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 19Charge difference: −4.0 C(−1.0)-N( 3.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 12)MITDISC:discrimination of mitochondrial targeting seqR content:3Hyd Moment (75):1.42Hyd Moment (95):2.81G content:3D/E content:1S/T content:3Score: −3.82Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 36 SRG|LLNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.6%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: cytoplasmic30.4%: mitochondrial13.0%: Golgi8.7%: endoplasmic reticulum 4.3%: extracellular, including cell wall 4.3%: nuclear 4.3%: vesicles of secretory system>> prediction for CG51027-06 is cyt (k = 23)


A search of the NOV11a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 11D.

TABLE 11DGeneseq Results for NOV11aNOV11aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE29924Human LP289 protein - Homo sapiens,1 . . . 336332/336 (98%)0.0336 aa. [WO200274906-A2, 26-SEP-2002]1 . . . 336332/336 (98%)ABG96271Human immunoglobulin superfamily1 . . . 336332/336 (98%)0.0protein IGSFP-9 - Homo sapiens, 3361 . . . 336332/336 (98%)aa. [WO200272794-A2, 19-SEP-2002]AAU84386Novel human secreted or membrane-1 . . . 336325/336 (96%)0.0associated protein #25 - Homo sapiens,1 . . . 336329/336 (97%)336 aa. [WO200204600-A2, 17-JAN-2002]ABG94639Human NOV5d protein - Homo sapiens,1 . . . 336322/336 (95%)0.0336 aa. [WO200266643-A2, 29-AUG-2002]1 . . . 336326/336 (96%)ABG94637Human NOV5b protein - Homo sapiens,18 . . . 336 315/319 (98%)0.0319 aa. [WO200266643-A2, 29-AUG-2002]1 . . . 319315/319 (98%)


In a BLAST search of public sequence databases, the NOV11a protein was found to have homology to the proteins shown in the BLASTP data in Table 11E.

TABLE 11EPublic BLASTP Results for NOV11aNOV11aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8HW98Adult male hypothalamus cDNA, RIKEN12 . . . 336313/325 (96%)0.0full-length enriched library, 1 . . . 325318/325 (97%)clone: A230106M20 product: weakly similarto limbic system-associated membraneprotein precursor (E19S) (CHLAMP, G19-isoform) - Mus musculus (Mouse), 325 aa(fragment).P11834Opioid binding protein/cell adhesion11 . . . 329168/328 (51%)1e−84molecule precursor (OBCAM) (Opioid-12 . . . 337224/328 (68%)binding cell adhesion molecule) (OPCML) -Bos taurus (Bovine), 345 aa.Q14982Opioid binding protein/cell adhesion11 . . . 329166/328 (50%)3e−84molecule precursor (OBCAM) (Opioid-12 . . . 337224/328 (67%)binding cell adhesion molecule) (OPCML) -Homo sapiens (Human), 345 aa.JC1238opioid-binding protein (clone DUZ1) - rat,12 . . . 333163/326 (50%)5e−83338 aa. 8 . . . 325221/326 (67%)P32736Opioid binding protein/cell adhesion11 . . . 333164/329 (49%)5e−83molecule precursor (OBCAM) (Opioid-12 . . . 332224/329 (67%)binding cell adhesion molecule) (OPCML) -Rattus norvegicus (Rat), 345 aa.


PFam analysis predicts that the NOV11a protein contains the domains shown in the Table 11F.

TABLE 11FDomain Analysis of NOV11aIdentities/SimilaritiesPfamNOV11afor theExpectDomainMatch RegionMatched RegionValueig 47 . . . 11416/72 (22%)0.0006540/72 (56%)ig147 . . . 19716/54 (30%)0.0005735/54 (65%)ig231 . . . 29312/66 (18%)2.7e-0545/66 (68%)


Example 12.

The NOV12 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 12A.

TABLE 12ANOV12 Sequence AnalysisNOV12a, CG51373-11SEQ ID NO: 3193212 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 2272ATGCTGAGCCTCCTCGTCTGGATCCTCACTCTCTCCGATACTTTCTCCCAAGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGAGCCTGGCTGGGGCATCTCTGCGGGGCAGAGGAGAAGGCTTTCGCAGCTGTTCCCTGATATTCAGGGACATTGCTCATTGCTCCCTTCTCGGACCAGCCTTCTTCCTCCCACCATGGCAGGTGGGGAGCAGGTCTCCCAGAGACACCCCGTCCCGAGGATGGTGCTCTGTGCATGCCCCAGCCTCCTGGGCCTGCCCTTCCCTCTTCTTCGGGAGGATGTGTCTCTTCTGACCTGCACTCTTGCCTGACCCTAGAATGGGGACAGGGAAAGTGAAGGTTAGGGAAAGCAGAGGGGGGCACTTTTTAGCATTCCCTTTCTATCCCACCCCTCTGATCTCCCATAAGTGGAAATGGGGGTACCCAGGGATGGGCAGGCTTTGGCCTAGGGACATGAAGTATGGGAGTGGGTGGCTGTGGCACAGACAGGTGGAAAACGGGATAGCCTGGCCAGTCCCTCTGTTGTCTGCATTCGTGCCCTGGGTGCCTCTCTCCTTCCTCAGGGTACTGCAGAAGGGAGCGAACAGGGTACTGTTCGCTCTTGTCTACAGAACAGCCCTGGCACTGCATTCAAATCCAGTCTTCATTCAGCTGGGATCAAAATGCCAGTCACCTTGGCTACCCACTGTGGACAGCTGTCTGTCAGCATGCAGAGGGATCCAGGAATCCCCCCGGCAGCACGGCCCGCTTTCCTTCTCCTCCATGCTGGGCCAGCCAGATAAGTCAGGGTCCTGGTGGAGAAAGAAAGGCTAGGACCATGTCCTCATTGACCCAGATACTGCTGTGTGCTGCACAGCAGTGAACCAACACTAGAGGGAGCCACACAAGCCTCCTCTCCCCAGTCTGCCCCACTTCCTGGCTTTAACTCTTGAGCTGGTTTGGGGAGTGGTGAGGTAGGGGTGGGGGTGCTGTAGGCTCTTTTTCAAAAAAAAACNOV12a, CG51373-11SEQ ID NO: 320757 aaMW at 83534.8kDProtein SequenceMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRIYIQTHVNOV12b, CG51373-10SEQ ID NO: 3213430 bpDNA SequenceORF Start: ATG at 351ORF Stop: TAG at 2490CTCTCCGATACTTTCTCCCAAGGGTCAGCTGCTTCTTCATTCCAAGTGGACAAGGAGCCAGCTGCTCACTGTCCTTGAGAGACTTCAGCGAGAGACCAGGGTGTCCAGGCTCCATGCAGGAAAGCCATGCGTATAAATTCCACCTCTGAGCCAGGCCTCACCAGCAAGCCCACTCTTAAGCCCTTGACTTGGGCTCCAGGGGCCATGGGAAGGAGAAACGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGGAAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATTGGGGCCCTGGCTTCTTGGTTCCCCACCCGAGGCTGCTCTCTCTGCCCAGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGAGCCTGGCTGGGGCATCTCTGCGGGGCAGAGGAGAAGGCTTTCGCAGCTGTTCCCTGATATTCAGGGACATTGCTCATTGCTCCCTTCTCGGACCAGCCTTCTTCCTCCCACCATGGCAGGTGGGGAGCAGGTCTCCCAGAGACACCCCGTCCCGAGGATGGTGCTCTGTGCATGCCCCAGCCTCCTGGGCCTGCCCTTCCCTCTTCTTCGGGAGGATGTGTCTCTTCTGACCTGCACTCTTGCCTGACCCTAGAATGGGGACAGGGAAAGTGAAGGTTAGGGAAAGCAGAGGGGGGCACTTTTTAGCATTCCCTTTCTATCCCACCCCTCTGATCTCCCATAAGTGGAAATGGGGCTACCCAGGGATGGGCAGGCTTTGGCCTAGGGACATGAAGTATGGGAGTGGGTGGCTGTGGCACAGACAGGTGGAAAACGGGATAGCCTGGCCAGTCCCTCTGTTGTCTGCATTCGTGCCCTGGGTGCCTCTCTCCTTCCTCAGGGTACTGCAGAAGGGAGCGAACAGGGTACTGTTCGCTCTTGTCTACAGAACAGCCCTGGCACTGCATTCAAATCCAGTCTTCATTCAGCTGGGATCAAAATGCCAGTCACCTTGGCTACCCACTGTGGACAGCTGTCTGTCAGCATGCAGAGGGATCCAGGAATCCCCCCGGCAGCACGGCCCGCTTTCCTTCTCCTCCATGCTGGGCCAGCCAGATAAGTCAGGGTCCTGGTGGAGAAAGAAAGGCTAGGACCATGTCCTCATTGACCCAGATACTGCTGTGTGCTGCACAGCAGTGAACCAACACTAGAGGGAGCCACACAAGCCTCCTCTCCCCAGTCTGCCCCACTTCCTGGCTTTAACTCTTGAGCTGGTTTGGGGAGTGGTGAGGTAGGGGTGGGGGTGCTGTAGGCTCTTTTTCAAAAAAAAACNOV12b, CG51373-10SEQ ID NO: 322713 aaMW at 78491.0kDProtein SequenceMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPETLGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGEIPPSLSPGPKRTQILGPWLLGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASTLLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12c, CG51373-08SEQ ID NO: 3232128 bpDNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119CAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12c, CG51373-08SEQ ID NO: 324696 aaMW at 76975.3kDProtein SequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNNVLSNSNQLLLKSVTQADAGTYTCHAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12d, 237582097SEQ ID NO: 3252100 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGAACACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGCTCGAGNOV12d, 237582097SEQ ID NO: 326700 aaMW at 77361.7kDProtein SequenceGSMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRPAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVLENOV12e, 294385961SEQ ID NO: 3272290 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTATGCTGAGCCTCCTCGTCTGGATCCTCACTCTCTCCGATACTTTCTCCCAAGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCGGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTCCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGGTCGACGGCNOV12e, 294385961SEQ ID NO: 328763 aaMW at 84148.5kDProtein SequenceTKLMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNNVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVVDGNOV12f, 294398021SEQ ID NO: 3292336 bpDNA SequenceORF Start: at 2ORF Stop: at 2336CACCAAGCTTATGCTGAGCCTCCTCGTCTGGATCCTCACTCTCTCCGATACTTTCTCCCAAGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGGGCCCAGGCCTCCTGGCTCCCCACCCGAGGCTGCTCTCTCTGCCCAGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGGTCGACGNOV12f, 294398021SEQ ID NO: 330778 aaMW at 85605.1kDProtein SequenceTKLMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQPAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVVDNOV12g, CG51373-01SEQ ID NO: 3311230 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 1003ATGCATTTGACTCTGGAAGTCTTAAACCATGGCCCCTTCCCTCTAAACCTTTCCTCCATTGCTTACAATCATGGAACTGTGTTTGGCCACTGGAAGAATAACGTCACTCGGGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGGAAGGAAACATTTCTGGTGAATGAGGAGGCAACGGGCGAGACCTCAGGAGACAATGTTGTTCATTCTAGGAATCTGTCTCAGACAATCTTCATCACCCGGAAACGATGGGAGGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCGTGAGTGGGCTGGGGGGAGCAGTCTGGAGCAGGGGGGTGGAAGAAGGGGTGTGTTTGAGAAGCACACTCTTAGTTTGAGAAACACAAACTAAGAGTCCCCCTATGGTCCCCAGGACAAACGCTTGCCTTCTTCACATCTTTCATTCCCTGGATTGAACCATGGGGACTAAGGGCTGGTAGAGCATTGGCTGTGGAGTCAGGCAGTCCCCAGGTCTAAACCAGCCTGTTATTAGTCAATGGTTTACACTCTCTGGGCCTCGGTTTCCAGTTCTGTATACTGTATATTGCAAAAGATAAAATACTGGCCTACAGCCCCANOV12g, CG51373-01SEQ ID NO: 332334 aaMW at 36655.8kDProtein SequenceMHLTLEVLNHGPFPLNLSSIAYNHGTVFGHWKNNVTRETLVKVKDAEDQLGARVGYIELDLNSGKETFLVNEEATGETSGDNVVHSRNLSQTIFITRKRWEGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTNNOV12h, CG51373-02SEQ ID NO: 3333464 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 2524ATGCATTTGACTCTGGAAGTCTTAAACCATGGCCCCTTCCCTCTAAACCTTTCCTCCATTGCTTACAATCATGGAACTGTGTTTGGCCACTGGAAGAATAACGTCACTCGGGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGGAAGGAAACATTTCTGGTGAATGAGGAGGCAACGGGCGAGACCTCAGGAGACAATGTTGTTCATTCTAGGAATCTGTCTCAGACAATCTTCATCACCCGGAAACGATGGGAGGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGAGCCTGGCTGGGGCATCTCTGCGGGGCAGAGGAGAAGGCTTTCGCAGCTGTTCCCTGATATTCAGGGACATTGCTCATTGCTCCCTTCTCGGACCAGCCTTCTTCCTCCCACCATGGCAGGTGGGGAGCAGGTCTCCCAGAGACACCCCGTCCCGAGGATGGTGCTCTGTGCATGCCCCAGCCTCCTGGGCCTGCCCTTCCCTCTTCTTCGGGAGGATGTGTCTCTTCTGACCTGCACTCTTGCCTGACCCTAGAATGGGGACAGGGAAAGTGAAGGTTAGGGAAAGCAGAGGGGGGCACTTTTTAGCATTCCCTTTCTATCCCACCCCTCTGATCTCCCATAAGTGGAAATGGGGGTACCCAGGGATGGGCAGGCTTTGGCCTAGGGACATGAAGTATGGGAGTGGGTGGCTGTGGCACAGACAGGTGGAAAACGGGATAGCCTGGCCAGTCCCTCTGTTGTCTGCATTCGTGCCCTGGGTGCCTCTCTCCTTCCTCAGGGTACTGCAGAAGGGAGCGAACAGGGTACTGTTCGCTCTTGTCTACAGAACAGCCCTGGCACTGCATTCAAATCCAGTCTTCATTCAGCTGGGATCAAAATGCCAGTCACCTTGGCTACCCACTGTGGACAGCTGTCTGTCAGCATGCAGAGGGATCCAGGAATCCCCCCGGCAGCACGGCCCGCTTTCCTTCTCCTCCATGCTGGGCCAGCCAGATAAGTCAGGGTCCTGGTGGAGAAAGAAAGGCTAGGACCATGTCCTCATTGACCCAGATACTGCTGTGTGCTGCACAGCAGTGAACCAACACTAGAGGGAGCCACACAAGCCTCCTCTCCCCAGTCTGCCCCACTTCCTGGCTTTAACTCTTGAGCTGGTTTGGGGAGTGGTGAGGTAGGGGTGGGGGTGCTGTAGGCTCTTTTTCAAAAAAAAACNOV12h, CG51373-02SEQ ID NO: 334841 aaMW at 92988.2kDProtein SequenceMHLTLEVLNHGPFPLNLSSIAYNHGTVFGHWKNNVTRETLVKVKDAEDQLGARVGYIELDLNSGKETFLVNEEATGETSGDNVVHSRNLSQTIFITRKRWEGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12i, CG51373-03SEQ ID NO: 3353379 bpDNA SequenceORF Start: ATG at 351ORF Stop: TAG at 2439CTCTCCGATACTTTCTCCCAAGGGTCAGCTGCTTCTTCATTCCAAGTGGACAAGGAGCCAGCTGCTCACTGTCCTTGAGAGACTTCAGCGAGAGACCAGGGTGTCCAGGCTCCATGCAGGAAAGCCATGCGTATAAATTCCACCTCTGAGCCAGGCCTCACCAGCAAGCCCACTCTTAAGCCCTTGACTTGGGCTCCAGGGGCCATGGGAAGGAGAAACGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGAGCCTGGCTGGGGCATCTCTGCGGGGCAGAGGAGAAGGCTTTCGCAGCTGTTCCCTGATATTCAGGGACATTGCTCATTGCTCCCTTCTCGGACCAGCCTTCTTCCTCCCACCATGGCAGGTGGGGAGCAGGTCTCCCAGAGACACCCCGTCCCGAGGATGGTGCTCTGTGCATGCCCCAGCCTCCTGGGCCTGCCCTTCCCTCTTCTTCGGGAGGATGTGTCTCTTCTGACCTGCACTCTTGCCTGACCCTAGAATGGGGACAGGGAAAGTGAAGGTTAGGGAAAGCAGAGGGGGGCACTTTTTAGCATTCCCTTTCTATCCCACCCCTCTGATCTCCCATAAGTGGAAATGGGGGTACCCAGGGATGGGCAGGCTTTGGCCTAGGGACATGAAGTATGGGAGTGGGTGGCTGTGGCACAGACAGGTGGAAAACGGGATAGCCTGGCCAGTCCCTCTGTTGTCTGCATTCGTGCCCTGGGTGCCTCTCTCCTTCCTCAGGGTACTGCAGAAGGGAGCGAACAGGGTACTGTTCGCTCTTGTCTACAGAACAGCCCTGGCACTGCATTCAAATCCAGTCTTCATTCAGCTGGGATCAAAATGCCAGTCACCTTGGCTACCCACTGTGGACAGCTGTCTGTCAGCATGCAGAGGGATCCAGGAATCCCCCCGGCAGCACGCCCCGCTTTCCTTCTCCTCCATGCTGGGCCAGCCAGATAAGTCAGGGTCCTGGTGGAGAAAGAAAGGCTAGGACCATGTCCTCATTGACCCAGATACTGCTGTGTGCTGCACAGCAGTGAACCAACACTAGAGGGAGCCACACAAGCCTCCTCTCCCCAGTCTGCCCCACTTCCTGGCTTTAACTCTTGAGCTGGTTTGGGGAGTGGTGAGGTAGGGGTGGGGGTGCTGTAGGCTCTTTTTCAAAAAAAAACNOV12i, CG51373-03SEQ ID NO: 336696 aaMW at 76928.3kDProtein SequenceMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDQRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12i, CG51373-04SEQ ID NO: 3371145 bpDNA SequenceORF Start: ATG at 351ORF Stop: TAA at 918CTCTCCGATACTTTCTCCCAAGGGTCAGCTGCTTCTTCATTCCAAGTGGACAAGGAGCCAGCTGCTCACTGTCCTTGAGAGACTTCAGCGAGAGACCAGGGTGTCCAGGCTCCATGCAGGAAAGCCATGCGTATAAATTCCACCTCTGAGCCAGGCCTCACCAGCAAGCCCACTCTTAAGCCCTTGACTTGGGCTCCAGGGGCCATGGGAAGGAGAAACGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCGTGAGTGGGCTGGGGGGAGCAGTCTGGAGCAGGGGGGTGGAAGAAGGGGTGTGTTTGAGAAGCACACTCTTAGTTTGAGAAACACAAACTAAGAGTCCCCCTATGGTCCCCAGGACAAACGCTTGCCTTCTTCACATCTTTCATTCCCTGGATTGAACCATGGGGACTAAGGGCTGGTAGAGCATTGGCTGTGGAGTCAGGCAGTCCCCAGGTCTAAACCAGCCTGTTATTAGTCAATGGTTTACACTCTCTGGGCCTCGGTTTCCAGTTCTGTATACTGTATATTGCAAAAGATAAAATACTGGCCTACAGCCCCANOV12j, CG51373-04SEQ ID NO: 338189 aaMW at 20595.8kDProtein SequenceMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTNNOV12k, CG51373-05SEQ ID NO: 3391148 bpDNA SequenceORF Start: ATG at 351ORF Stop: TAA at 918CTCTCCGATACTTTCTCCCAAGGGTCAGCTGCTTCTTCATTCCAAGTGGACAAGGAGCCAGCTGCTCACTGTCCTTGAGAGACTTCAGCGAGAGACCAGGGTGTCCAGGCTCCATGCAGGAAAGCCATGCGTATAAATTCCACCTCTGAGCCAGGCCTCACCAGCAAGCCCACTCTTAAGCCCTTGACTTGGGCTCCAGGGGCCATGGGAAGGAGAAACGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCGTGAGTGGGCTGGGGGGAGCAGTCTGGAGCAGGGGGGTGGAAGAAGGGGTGTGTTTGAGAAGCACACTCTTAGTTTGAGAAACACAAACTAAGAGTCCCCCTATGGTCCCCAGGACAAACGCTTGCCTTCTTCACATCTTTCATTCCCTGGATTGAACCATGGGGACTAAGGGCTGGTAGAGCATTGGCTGTGGAGTCAGGCAGTCCCCAGGTCTAAACCAGCCTGTTATTAGTCAATGGTTTACACTCTCTGGGCCTCGGTTTCCAGTTCTGTATACTGTATATTGCAAAAGATAAAATACTGGCCTACAGCCCCAAAANOV12k, CG51373-05SEQ ID NO: 340189 aaMW at 20595.8kDProtein SequenceMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTNNOV12l, CG51373-06SEQ ID NO: 3411148 bpDNA SequenceORF Start: ATG at 351ORF Stop: TAA at 918CTCTCCGATACTTTCTCCCAAGGGTCAGCTGCTTCTTCATTCCAAGTGGACAAGGAGCCAGCTGCTCACTGTCCTTGAGAGACTTCAGCGAGAGACCAGGGTGTCCAGGCTCCATGCAGGAAAGCCATGCGTATAAATTCCACCTCTGAGCCAGGCCTCACCAGCAAGCCCACTCTTAAGCCCTTGACTTGGGCTCCAGGGGCCATGGGAAGGAGAAACGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCGTGAGTGGGCTGGGGGGAGCAGTCTGGAGCAGGGGGGTGGAAGAAGGGGTGTGTTTGAGAAGCACACTCTTAGTTTGAGAAACACAAACTAAGAGTCCCCCTATGGTCCCCAGGACAAACGCTTGCCTTCTTCACATCTTTCATTCCCTGGATTGAACCATGGGGACTAAGGGCTGGTAGAGCATTGGCTGTGGAGTCAGGCAGTCCCCAGGTCTAAACCAGCCTGTTATTAGTCAATGGTTTACACTCTCTGGGCCTCGGTTTCCAGTTCTGTATACTGTATATTGCAAAAGATAAAATACTGGCCTACAGCCCCAAAANOV12l, CG51373-06SEQ ID NO: 342189 aaMW at 20595.8kDProtein SequenceMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTNNOV12m, CG51373-07SEQ ID NO: 3431407 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGCCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGANOV12m, CG51373-07SEQ ID NO: 344469 aaMW at 51246.2kDProtein SequenceRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEERNOV12n, CG51373-09SEQ ID NO: 3452128 bpDNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119CAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12n, CG51373-09SEQ ID NO: 346696 aaMW at 76975.3kDProtein SequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTNVNOV12o, CG51373-12SEQ ID NO: 3472336 bpDNA SequenceORF Start: ATG at 11ORF Stop: at 2330CACCAAGCTTATGCTGAGCCTCCTCGTCTGGATCCTCACTCTCTCCGATACTTTCTCCCAAGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGGGCCCAGGCCTCCTGGCTCCCCACCCGAGGCTGCTCTCTCTGCCCAGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGGTCGACGNOV12o, CG51373-12SEQ ID NO: 348773 aaMW at 85048.5kDProtein SequenceMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRHAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDTGSDVTLTCVWVGNPPLTLTWTKKDSNMGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRPRKGSRKDVTLRXLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12p, CG51373-13SEQ ID NO: 3492290 bpDNA SequenceORF Start: ATG at 11ORF Stop: at 2282CACCAAGCTTATGCTGAGCCTCCTCGTCTGGATCCTCACTCTCTCCGATACTTTCTCCCAAGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTAACAGCCAACCCGGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTCCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGGTCGACGGCNOV12p, CG51373-13SEQ ID NO: 350757 aaMW at 83534.8kDProtein SequenceMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAXPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12q, CG51373-14SEQ ID NO: 3512290 bpDNA SequenceORF Start: at 11ORF Stop: at 2282CACCAAGCTTCAAGGGACCCAGACCCGCTTCAGCCAGGAGCCAGCTGACCAGACGGTGGTGGCTGGACAGCGGGCCGTGCTCCCCTGTGTGCTGCTCAACTACTCTGGAATTGTGCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCGGAGATCTTGGGCTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGGGCCCAGGCCTCCTGGCTCCCCACCCGAGGCTGCTCTCTCTGCCCAGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTAAGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGGTCGACGGCNOV12q, CG51373-14SEQ ID NO: 352757 aaMW at 83227.3kDProtein SequenceQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRPAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12r, SNP13377352 ofSEQ ID NO: 3532128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 487SNP Change: T to CCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTCCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12r, SNP13377352 ofSEQ ID NO: 354696 aaMW at 76985.3kDCG51373-08, ProteinSNP Pos: 153SNP Change: Ser to ProSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETPIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYEAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12s, SNP13379746 ofSEQ ID NO: 3552128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 597SNP Change: C to GCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCGGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12s, SNP13379746 ofSEQ ID NO: 356696 aaMW at 76975.3kDCG51373-08, ProteinSNP Pos: 189SNP Change: Pro to ProSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYEAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12t, SNP13374859 ofSEQ ID NO: 3572128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 961SNP Change: C to TCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCTGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12t, SNP13374859 ofSEQ ID NO: 358696 aaMW at 77005.3kDCG51373-08, ProteinSNP Pos: 311SNP Change: Arg to TrpSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCWAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12u, SNP13374858 ofSEQ ID NO: 3592128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 1340SNP Change: T to CCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCACAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12u, SNP13374858 ofSEQ ID NO: 360696 aaMW at 76963.2kDCG51373-08, ProteinSNP Pos: 437SNP Change: Ile to ThrSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNNVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGITAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12v, SNP13374856 ofSEQ ID NO: 3612128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 1418SNP Change: G to ACAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGACGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCACAAAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12v, SNP13374856 ofSEQ ID NO: 362696 aaMW at 76956.2kDCG51373-08, ProteinSNP Pos: 463SNP Change: Arg to HisSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRPAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCEAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRHKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12w, SNP13374855 ofSEQ ID NO: 3632128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 1421SNP Change: A to GCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAGAGGCAGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12w, SNP13374855 ofSEQ ID NO: 364696 aaMW at 77003.3kDCG51373-08, ProteinSNP Pos: 464SNP Change: Lys to ArgSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRRGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAEEDRPSSEAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVJNOV12x, SNP13374854 ofSEQ ID NO: 3652128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 1426SNP Change: A to TCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCTGTCGCAAAGACGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12x, SNP13374854 ofSEQ ID NO: 366696 aaMW at 76991.4kDCG51373-08, ProteinSNP Pos: 466SNP Change: Ser to CysSequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGCRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHVNOV12y, SNP13374853 ofSEQ ID NO: 3672128 bpCG51373-08, DNA SequenceORF Start: ATG at 31ORF Stop: TAG at 2119SNP Pos: 1436SNP Change: A to GCAATGGACCAAGGACGGGCTGGCCCTGGGCATGGGCCAGGGCCTCAAAGCCTGGCCACGGTACCGGGTTGTGGGCTCCGCAGACGCTGGGCAGTACAACCTGGAGATCACAGATGCTGAGCTCTCTGACGACGCCTCTTACGAGTGCCAGGCCACGGAGGCCGCCCTGCGCTCTCGGCGGGCCAAACTCACCGTGCTCATCCCCCCAGAGGACACCAGGATTGACGGAGGCCCTGTGATTCTACTGCAGGCAGGCACCCCCCACAACCTCACATGCCGGGCCTTCAATGCGAAGCCTGCTGCCACCATCATCTGGTTCCGGGACGGGACGCAGCAGGAGGGCGCTGTGGCCAGCACGGAATTGCTGAAGGATGGGAAGAGGGAGACCACCGTGAGCCAACTGCTTATTAACCCCACGGACCTGGACATAGGGCGTGTCTTCACTTGCCGAAGCATGAACGAAGCCATCCCTAGTGGCAAGGAGACTTCCATCGAGCTGGATGTGCACCACCCTCCTACAGTGACCCTGTCCATTGAGCCACAGACGGTGCAGGAGGGTGAGCGTGTTGTCTTTACCTGCCAGGCCACAGCCAACCCCGAGATCTTGGACTACAGGTGGGCCAAAGGGGGTTTCTTGATTGAAGACGCCCACGAGAGTCGCTATGAGACAAATGTGGATTATTCCTTTTTCACGGAGCCTGTGTCTTGTGAGGTTCACAACAAAGTGGGAAGCACCAATGTCAGCACTTTAGTAAATGTCCACTTTGCTCCCCGGATTGTAGTTGACCCCAAACCCACAACCACAGACATTGGCTCTGATGTGACCCTTACCTGTGTCTGGGTTGGGAATCCCCCCCTCACTCTCACCTGGACCAAAAAGGACTCAAATATGGTCCTGAGTAACAGCAACCAGCTGCTGCTGAAGTCGGTGACTCAGGCAGACGCTGGCACCTACACCTGCCGGGCCATCGTGCCTCGAATCGGAGTGGCTGAGCGGGAGGTGCCGCTCTATGTGAACGGGCCCCCCATCATCTCCAGTGAGGCAGTGCAGTATGCTGTGAGGGGTGACGGTGGCAAGGTGGAGTGTTTCATTGGGAGCACACCACCCCCAGACCGCATAGCATGGGCCTGGAAGGAGAACTTCTTGGAGGTGGGGACCCTGGAACGCTATACAGTGGAGAGGACCAACTCAGGCAGTGGGGTGCTATCCACGCTCACCATCAACAATGTCATGGAGGCCGACTTTCAGACTCACTACAACTGCACCGCCTGGAACAGCTTCGGGCCAGGCACAGCCATCATCCAGCTGGAAGAGCGAGAGGTGTTACCTGTGGGCATCATAGCTGGGGCCACCATCGGCGCGAGCATCCTGCTCATCTTCTTCTTCATCGCCTTGGTATTCTTCCTCTACCGGCGCCGCAAAGGCAGTCGCAAAGGCGTGACCCTGAGGAAGCTGGATATCAAGGTGGAGACAGTGAACCGAGAGCCACTTACGATGCATTCTGACCGGGAGGATGACACCGCCAGCGTCTCCACAGCAACCCGGGTCATGAAGGCCATCTACTCGTCGTTTATGGATGATGTGGATCTGAAGCAGGACCTGCGCTGCGACACCATCGACACCCGGGAGGAGTATGAGATGAAGGACCCCACCAATGGCTACTACAACGTGCGTGCCCATGAAGACCGCCCGTCTTCCAGGGCAGTGCTCTATGCTGACTACCGTGCCCCTGGCCCTGCCCGCTTCGACGGCCGCCCCTCATCCCGTCTCTCCCACTCCAGCGGCTATGCCCAGCTCAACACCTATAGCCGGGGCCCTGCCTCTGACTATGGCCCTGAGCCCACACCCCCTGGCCCTGCTGCCCCAGCTGGCACTGACACAACCAGCCAGCTGTCCTACGAGAACTATGAGAAGTTCAACTCCCATCCCTTCCCTGGGGCAGCTGGGTACCCCACCTACCGACTGGGCTACCCCCAGGCCCCACCCTCTGGCCTGGAGCGGACCCCATATGAGGCGTATGACCCCATTGGCAAGTACGCCACAGCCACTCGATTCTCCTACACCTCCCAGCACTCGGACTACGGCCAGCGATTCCAGCAGCGCATGCAGACTCACGTGTAGGGGCCAGNOV12y, SNP13374853 ofSEQ ID NO: 368696 aaMW at 76917.3kDCG51373-08, ProteinSNP Pos: 469SNP Change: Asp to GlySequenceMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSNNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKGVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVEAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 12B.

TABLE 12BComparison of the NOV12 protein sequences.NOV12a ------------------------------------------------------------NOV12b ------------------------------------------------------------NOV12c ------------------------------------------------------------NOV12d ------------------------------------------------------------NOV12e ------------------------------------------------------------NOV12f ------------------------------------------------------------NOV12g MHLTLEVLNHGPFPLNLSSIAYNHGTVFGHWKNNVTRETLVKVKDAEDQLGARVGYIELDNOV12h MHLTLEVLNHGPFPLNLSSIAYNHGTVFGHWKNNVTRETLVKVKDAEDQLGARVGYIELDNOV12i ------------------------------------------------------------NOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m ------------------------------------------------------------NOV12n ------------------------------------------------------------NOV12o ------------------------------------------------------------NOV12p ------------------------------------------------------------NOV12q ------------------------------------------------------------NOV12a -------------------------MLSLLVWILTLSDTFSQGTQTRFSQEPADQWVAGQNOV12b ------------------------------------------------------------NOV12c ------------------------------------------------------------NOV12d ------------------------------------------------------------NOV12e ---------------------TKLMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQNOV12f ---------------------TKLMLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQNOV12g LNSGKETFLVNEEATGETSGDNVVBSRNLSQTIFITRKRWEGTQTRFSQEPADQTVVAGQNOV12h LNSGKETFLVNEEATGETSGDNVVHSRNLSQTIFITRKRWEGTQTRFSQEPADQTVVAGQNOV12i ------------------------------------------------------------NOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m ---------------------------------------------RFSQEPADQTVVAGQNOV12n ------------------------------------------------------------NOV12o ------------------------MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQNOV12p ------------------------MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQNOV12q ----------------------------------------QGTQTRFSQEPADQTVVAGQNOV12a RAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12b -------------------------MGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12c -------------------------MGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12d -----------------------GSMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12e RAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12f RAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12g RAVLPCVLLNYSGIVQWTKDGLALGMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12h RAVLPCVLLNYSGIVQWTKDGLALGMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12i -------------------------MGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12j -------------------------MGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12k -------------------------MGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12l -------------------------MGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12m RAVLPCVLLNYSGIVQWTKDGLALGMGQALKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12n -------------------------MGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12o RAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12p RAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12q RAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDANOV12a SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12b SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12c SYECQATEAALRSRRAXLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12d SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCEAFNAKPAATIIWNOV12e SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCEAFNAKPAATIIWNOV12f SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12g SYECQATEAALRSRRAXLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12h SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCEAFNAKPAATIIWNOV12i SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12j SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12k SYECQATEAALRSRPAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12l SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12m SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12n SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12o SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12p SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCHAFNAKPAATIIWNOV12q SYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWNOV12a FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12b FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12c FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12d FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12e FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12f FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12g FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12h FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12i FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12j FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12k FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12l FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12m FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12n FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSNNEAIPSGKETSIENOV12o FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12p FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12q FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIENOV12a LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12b LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12c LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVNOV12d LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVNOV12e LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12f LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12g LDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTN--------------------------NOV12h LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12i LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12j LDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTN--------------------------NOV12k LDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTN--------------------------NOV12l LDVHREWAGGSSLEQGGGRRGVFEKHTLSLRNTN--------------------------NOV12m LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12n LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILDYRWAKGGFLIEDAHESRYETNVNOV12o LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12p LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12q LDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVNOV12a DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12b DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGEIPNOV12c DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12d DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12e DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12f DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12g ------------------------------------------------------------NOV12h DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12i DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12n DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12o DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12p DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12q DYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPNOV12a LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCPAIVPRIGNOV12b PSLSPGPKRTQILGPWLLGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12c LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12d LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12e LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12f LTLTWTKKDSN-MGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCPAIVPRIGNOV12g ------------------------------------------------------------NOV12h LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12i LTLTWTKK-----------------DSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCRATVPRIGNOV12n LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12o LTLTWTKKDSN-MGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12p LTLTWTKKD-----------------SNMVLSNSNQLLLKSVTQADAGTYTCEAIVPRIGNOV12q LTLTWTKKDSN-MGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGNOV12a VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12b VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12c VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12d VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12e VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12f VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12g ------------------------------------------------------------NOV12h VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12i VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12n VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12o VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12p VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12q VAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLENOV12a RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12b RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12c RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12d RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12e RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12f RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12g ------------------------------------------------------------NOV12h RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12i RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEER---------NOV12n RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12o RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12p RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12q RYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIANOV12a GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12b GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12c GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12d GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12e GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12f GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12g ------------------------------------------------------------NOV12h GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12i GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m ------------------------------------------------------------NOV12n GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12o GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12p GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12q GATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTANOV12a SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12b SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVPAHEDRPSSRANOV12c SVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12d SVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSEANOV12e SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSEANOV12f SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12g ------------------------------------------------------------NOV12h SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12i SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m ------------------------------------------------------------NOV12n SVSTATRVMKAIYSSFMDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12o SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12p SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12q SVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRANOV12a VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12b VLYADYPAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12c VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12d VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12e VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYQPEPTPPGPAAPAGTDTTNOV12f VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12g ------------------------------------------------------------NOV12h VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12i VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m ------------------------------------------------------------NOV12n VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12o VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12p VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12q VLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTNOV12a SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12b SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12c SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12d SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12e SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12f SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12g ------------------------------------------------------------NOV12h SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12i SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12j ------------------------------------------------------------NOV12k ------------------------------------------------------------NOV12l ------------------------------------------------------------NOV12m ------------------------------------------------------------NOV12n SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12o SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12p SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12q SQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTNOV12a SQHSDYGQRFQQRMQTHV---NOV12b SQHSDYGQRFQQRMQTHV---NOV12c SQHSDYGQRFQQRMQTHV---NOV12d SQHSDYGQRFQQRMQTHVLE-NOV12e SQHSDYGQRFQQRMQTHVVDGNOV12f SQHSDYGQRFQQRMQTHVVD-NOV12g ---------------------NOV12h SQHSDYGQRFQQRMQTHV---NOV12i SQHSDYGQRFQQRMQTHV---NOV12j ---------------------NOV12k ---------------------NOV12l ---------------------NOV12m ---------------------NOV12n SQHSDYGQRFQQRMQTHV---NOV12o SQHSDYGQRFQQRMQTHV---NOV12p SQHSDYGQRFQQRMQTHV---NOV12q SQHSDYGQRFQQRMQTHV---NOV12a (SEQ ID NO: 320)NOV12b (SEQ ID NO: 322)NOV12c (SEQ ID NO: 324)NOV12d (SEQ ID NO: 326)NOV12e (SEQ ID NO: 328)NOV12f (SEQ ID NO: 330)NOV12g (SEQ ID NO: 332)NOV12h (SEQ ID NO: 334)NOV12i (SEQ ID NO: 336)NOV12j (SEQ ID NO: 338)NOV12k (SEQ ID NO: 340)NOV12l (SEQ ID NO: 342)NOV12m (SEQ ID NO: 344)NOV12n (SEQ ID NO: 346)NOV12o (SEQ ID NO: 348)NOV12p (SEQ ID NO: 350)NOV12q (SEQ ID NO: 352)


Further analysis of the NOV12a protein yielded the following properties shown in Table 12C.

TABLE 12CProtein Sequence Properties NOV12aSignalP analysis:Cleavage site between residues 17 and 18PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 12; peak value 10.45PSG score:6.05GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 0.73possible cleavage site: between 16 and 17>>> Seems to have a cleavable signal peptide (1 to 16)ALOM:Klein et al's method for TM region allocationInit position for calculation: 17Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 503-519−12.26PERIPHERALLikelihood = 3.61 (at 38)ALOM score: −12.26 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 8Charge difference: −2.0 C(−1.0)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 520 to 757)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):1.68Hyd Moment(95):2.60G content:1D/E content:2S/T content:8Score: −5.22Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 32 TRF|SQNUCDISC: discrimination of nuclear localization signalspat4: RRRK (5) at 522pat7: nonebipartite: nonecontent of basic residues: 9.6%NLS Score: −0.16KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: endoplasmic reticulum22.2%: Golgi22.2%: extracellular, including cell wall11.1%: plasma membrane>> prediction for CG51373-11 is end (k = 9)


A search of the NOV12a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 12D.

TABLE 12DGeneseq Results for NOV12aNOV12aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABB05749Human G protein-coupled receptor17 . . . 757739/741 (99%)0.0NOV1a protein SEQ ID NO: 2 - Homo101 . . . 841 740/741 (99%)sapiens, 841 aa. [WO200200691-A2,03-JAN-2002]ABP66884Human polypeptide SEQ ID NO 605 -57 . . . 757701/717 (97%)0.0Homo sapiens, 749 aa.33 . . . 749701/717 (97%)[US2002090672-A1, 11-JUL-2002]ABB10297Human cDNA SEQ ID NO: 605 - Homo57 . . . 757701/717 (97%)0.0sapiens, 749 aa. [WO200154474-A2,33 . . . 749701/717 (97%)02-AUG-2001]ABB05762Human G protein-coupled receptor62 . . . 757695/696 (99%)0.0NOV7b protein SEQ ID NO: 26 - Homo 1 . . . 696695/696 (99%)sapiens, 696 aa. [WO200200691-A2,03-JAN-2002]ABG65107Human albumin fusion protein #1782 -62 . . . 757696/712 (97%)0.0Homo sapiens, 712 aa. [WO200177137- 1 . . . 712696/712 (97%)A1, 18-OCT-2001]


In a BLAST search of public sequence databases, the NOV12a protein was found to have homology to the proteins shown in the BLASTP data in Table 12E.

TABLE 12EPublic BLASTP Results for NOV12aNOV12aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueCAD23338Sequence 1 from Patent17 . . . 757739/741 (99%)0.0WO0200691 - Homo sapiens101 . . . 841 740/741 (99%)(Human), 841 aa.AAO91769NEPH1 - Mus musculus (Mouse),18 . . . 757717/740 (96%)0.0789 aa.50 . . . 789728/740 (97%)Q8CJ59NEPH1 - Mus musculus (Mouse),18 . . . 757714/740 (96%)0.0789 aa.50 . . . 789726/740 (97%)CAD23349Sequence 25 from Patent62 . . . 757695/696 (99%)0.0WO0200691 - Homo sapiens 1 . . . 696695/696 (99%)(Human), 696 aa.AAP12626NEPH1 - Rattus norvegicus (Rat),53 . . . 754678/702 (96%)0.0702 aa (fragment). 1 . . . 702688/702 (97%)


PFam analysis predicts that the NOV12a protein contains the domains shown in the Table 12F.

TABLE 12FDomain Analysis of NOV12aIdentities/SimilaritiesPfamNOV12afor the MatchedExpectDomainMatch RegionRegionValueig 35 . . . 10212/72 (17%)1.3e−0547/72 (65%)ig136 . . . 20214/69 (20%)0.002348/69 (70%)ig322 . . . 37321/55 (38%)2.2e−0943/55 (78%)


Example 13

The NOV13 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 13A.

TABLE 13ANOV13 Sequence AnalysisNOV13a, CG51622-04SEQ ID NO: 3691826 bpDNA SequenceORF Start: ATG at 167ORF Stop: TGA at 1721AGGACTCCAGCGTGCCCAGTGACATAAACCCACTCTGCACATACCAGTTGCTCTGTCCCAGCTGTGCTGCGGTGGTGCTGGGACAAAAGAAGCATGAGACGCAGACTCTGTCCCTCAAAGAACTCCCAGTGCAGGACAATGTGGTGCCCAAGGAAGACCCAGGGACATGGGAGTGGAGGACAGTCAAAGAAGGGATCCTGGAAGAGGTCTGGCATCCTGCACTTGCTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACATCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGTCCTGTTGGACTCTGTGCTTCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAAAGGCTGCAGATAAGCTGGGATCTACCCAGATCGTGAAGATCCTAACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGCCCAACTGATCGTGCTGGAAGTGTTTCCCTCCAGTGAAGCCCTCCGCCCTTTGTTCACCCTGGGCATCGAAGCCAGCTCGGAAGCTCAGTTTTACACCAAAGGTGACCAACTTATACTCAACTTGAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAATGCCCCTTCTCTCTCTCACAGCCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTGCCGAACCAGAATGGCAAATTAAGATCTGGGGTCCCAGTGTCATTGGTGAAGGCCTTGGGCCAGCTCTCCTGTCTCCCAGTGAAGACTTGGATGGCAGCCATCAGGGAAGGCTGGGTCCCAGCTGGGAGTATGGGTGTGAGCTCTATAGACCATCCCTCTCTGCAATCAATAAACACTTGCCTGTGNOV13a, CG51622-04Protein SequenceSEQ ID NO: 370518 aaMW at 56020.9kDMGVEDSQRRDPGRGLASCTCCPLTPGKMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHIIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQCPFSLSQPDVLKNIITEIIHSILLPNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13b, CG51622-03SEQ ID NO: 3711452 bpDNA SequenceORF Start: ATG at 44ORF Stop: TGA at 1346AGGTCTGCCATCCTGCACTTGCTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCCTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCCATGCCAAGGTGGCCCAACTGATCGTGCTGGAAGTGTTTCCCTCCAGTGAAGCCCTCCGCCCTTTGTTCACCCTGGGCATCGAAGCCAGCTCGGAAGCTCAGTTTTACACCAAAGGTGACCAACTTATACTCAACTTGAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTGCCGAACCAGAATGGCAAATTAAGATCTGGGGTCCCAGTGTCATTGGTGAAGGCCTTGGGATTCGAGGCAGCTGAGTCCTCACTGAGCAAGGATGCCCTTGTGCTTACTCCAGCCTCCTTGTGGAAACCCACCTCTCCTGTCTCCCAGTGAAGACTTGGATGGCAGCCATCAGGGAAGGCTGGGTCCCAGCTGGGAGTATGGGTGTGAGCTCTATAGACCATCCCTCTCTGCAATCAATAAACACTTGCCTGTGANOV13b, CG51622-03Protein SequenceSEQ ID NO: 3721434 aaMW at 46965.5kDMAGPWTFTPLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASSEAQFYTKGDQLILNLNNISSDRIQLNNSGIGWFQPDVLKNIITEIIHSILLPNQNGKLRSGVPVSLVKALGFEAAESSLSKDALVLTPASLWKPTSPVSQNOV13c, CG51622-05SEQ ID NO: 3731580 bpDNA SequenceORF Start: ATG at 77ORF Stop: TGA at 1475AGAGAGGAGCGGGCCGAGGACTCCAGCGTGCCCAGGTCTGGCATCCTGCACTTGCTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGCTTCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAAAGGCTGCAGATAAGCTGGGATCTACCCAGATCGTGAAGATCCTAACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGCCCAACTGATCGTGCTGGAAGTGTTTCCCTCCAGTGAAGCCCTCCGCCCTTTGTTCACCCTGGGCATCGAAGCCAGCTCGGAAGCTCAGTTTTACACCAAAGGTGACCAACTTATACTCAACTTGAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTGCCGAACCAGAATGGCAAATTAAGATCTGGGGTCCCAGTGTCATTGGTGAAGGCCTTGGGATTCGAGGCAGCTGAGTCCTCACTGAGCAAGGATGCCCTTGTGCTTACTCCAGCCTCCTTGTGGAAACCCACCTCTCCTGTCTCCCAGTGAAGACTTGGATGGCAGCCATAGGGAAGGCTGGGTCCCAGCTGGGAGTATGGGTGTGAGCTCTATAGACCATCCCTCTCTGCAATCAATAAACACTTGCCTGTGNOV13c, CG51622-05Protein SequenceSEQ ID NO: 374466 aaMW at 50460.5kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQPDVLKNIITEIIHSILLPNQNGKLRSGVPVSLVKALGFEAAESSLSKDALVLTPASLWKPTSPVSQNOV13d, CG51622-01SEQ ID NO: 375963 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 961ATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAGTCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACATCACCCCATCCAGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGGTAGGTGCTCTGCTCTCTCTCCCACTTTTTCCTTTACTACGGAGCTGGCCTCCAGACCCGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCTAANov13d, CG51622-01Protein SequenceSEQ ID NO: 376320 aaMW at 34533.2kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKESLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHITPSRLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKGRCSALSPTFSFTTELASRPGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCPNOV13e, 275698370SEQ ID NO: 3771024 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCCTCGAGGGCNOV13e, 275698370Protein SequenceSEQ ID NO: 378341 aaMW at 36955.1kDTGSTMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCPLEIGNOV13f, 274054187SEQ ID NO: 379946 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCATCTGGCTGAAGGTTCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCCTCGAGNOV13f, 274054187Protein SequenceSEQ ID NO: 380315 aaMW at 34322.0kDTGSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCPLENOV13g, CG51622-02SEQ ID NO: 3811035 bpDNA SequenceORF Start: ATG at 79ORF Stop: TAA at 1033AGAGCGTTGGGTCACGTGAGGACTCCAGCGTGCCCAGGTCTGGCATCCTGCACTTACTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACATCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGGTAGGTGCTCTGCTCTCTCTCCCACTTTTTCCTTTACTACGGAGCTGGCCTCCAGACCCGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCTAANOV13g, CG51622-02Protein SequenceSEQ ID NO: 382318 aaMW at 34432.2kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHIIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKGRCSALSPTFSFTTELASRPGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVIVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCPNOV13h, CG51622-06SEQ ID NO: 3831572 bpDNA SequenceORF Start: ATG at 124ORF Stop: TGA at 1441GGTGTGCAGGATATAAGGTTGGACTTCCAGACCCACTGCCCGGGAGAGGAGAGGAGCGGGCCGAGGACTCCAGCGTGCCCAGGTCTGGCATCCTGCACTTGCTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACATCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCTTGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCACCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGCTTCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAAAGGAAGCCAGCTCGGAAGCTCAGTTTTACACCAAAGGTGACCAACTTATACTCAACTTGAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTCAGCTGAGTCCTCACTGACCAAGGATGCCCTTGTGCTTACTCCAGCCTCCTTGTGGAAACCCAGCTCTCCTGTCTCCCAGTGAAGACTTGGATGGCAGCCATCAGGGAAGGCTGGGTCCCAGTTGGGAGTATGGGTGTGAGCTCTATAGACCATCCCTCTCTGCAATCAATAAACACTTGCCTGTGAAAAAAAAAAAAAAATAAAAAAAAAANOV13h, CG51622-06Protein SequenceSEQ ID NO: 384439 aaMW at 47572.2kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHIIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLXTSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSHPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKEASSELGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13i, CG51622-07SEQ ID NO: 3851023 bpDNA SequenceORF Start: ATG at 19ORF Stop: TAA at 1021CCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCTAANOV13l, CG51622-07Protein SequenceSEQ ID NO: 386334 aaMW at 36309.5kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCPNOV13j, CG51622-08SEQ ID NO: 3871509 bpDNA SequenceORF Start: ATG at 31ORF Stop: TGA at 1480TGCACTTGCTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACATCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGCTTCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAAAGGCTGCAGATAAGCTGGGATCTACCCAGATCGTGAAGATCCTAACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGCCCAACTGATCGTGCTGGAAGTGTTTCCCTCCAGTGAAGCCCTCCGCCCTTTGTTCAAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTGCCGAACCAGAATGGCAAATTAAGATCTGGGGTCCCAGTGTCATTGGTGAAGGCCTTGGGATTCGAGGCAGCTGAGTCCTCACTGACCAAGGATGCCCTTGTGCTTACTCCAGCCTCCTTGTGGAAACCCAGCTCTCCTGTCTCCCAGTGAAGACTTGGATGGCAGCCATCAGGGAAGNOV13j, CG51622-08Protein SequenceSEQ ID NO: 3881483 aaMW at 52327.7kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVTKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHIIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQPDVLKNIITEIIHSILLPNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13k, CG51622-09SEQ ID NO: 3891392 bpDNA SequenceORF Start: at 7ORF Stop: at 1387AAGCTTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAACACATCATCTGGCTGATGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCGTCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCCTCAGCATTGACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGCTTCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAAAGGCTGCAGATAAGCTGGGACCTACCCAGATCGTGAAGATCCTAACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGCCCAACTGATCGTGCTGGAAGTGTTTCCCTCCAGTGAAGCCCTCCGCCCTTTGTTCACCCTGGGCATCGAAGCCAGCTCGGAAGCTCAGTTTTACACCAAAGGTGACCAACTTATACTCAACTTGAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTGCCGAACCAGAATGGCAAATTAAGATCTGGGGTCCCAGTGTCATTGGTGAAGGCCTTGGGATTCGAGGCAGCTGAGTCCTCACTGACCAAGGATGCCCTTGTGCTTACTCCAGCCTCCTTGTGGAAACCCAGCTCTCCTGTCTCCCAGCTCGAGNOV13k, CG51622-09Protein SequenceSEQ ID NO: 390460 aaMW at 49975.9kDPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSANREKPAGGIPVLGSLVNTVLKHIIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKAADKLGPTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQPDVLKNIITEIIHSILLPNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13l, CG51622-10SEQ ID NO: 3911684 bpDNA SequenceORF Start: ATG at 77ORF Stop: TGA at 1529AGAGAGGAGCGGGCCGAGGACTCCAGCGTGCCCAGGTCTGGCATCCTGCACTTGCTGCCCTCTGACACCTGGGAAGATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAAGCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCATCTGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCGTGATCTGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGTGCCCATTTCCTCAGCATTGAACCGTCTGGAGTTTGACCTTCTGTATCCTGCCATCAAGGGTGACACCATTCAGCTCTACCTGGGGGCCAAGTTGTTGGACTCACAGGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGCTTCCTGAGAGTGCCCATCGGCTGAAGTCAAGCATCGGGCTGATCAATGAAAAGGCTGCAGATAAGCTGGGATCTACCCAGATCGTGAAGATCCTAACTCAGGACACTCCCGAGTTTTTTATAGACCAAGGCCATGCCAAGGTGGCCCAACTGATCGTGCTGGAAGTGTTTCCCTCCAGTGAAGCCCTCCGCCCTTTGTTCACCCTGGGCATCGAAGCCAGCTCGGAAGCTCAGTTTTACACCAAAGGTGACCAACTTATACTCAACTTGAATAACATCAGCTCTGATCGGATCCAGCTGATGAACTCTGGGATTGGCTGGTTCCAACCTGATGTTCTGAAAAACATCATCACTGAGATCATCCACTCCATCCTGCTGCCGAACCAGAATGGCAAATTAAGATCTGGGGTCCCAGTGTCATTGGTGAAGGCCTTGGGATTCGAGGCAGCTGAGTCCTCACTGAGCAAGGATGCCCTTGTGCTTACTCCAGCCTCCTTGTGGAAACCCACCTCTCCTGTCTCCCAGTGAAGACTTGGATGGCAGCCATCAGGGAAGGCTGGGTCCCAGCTGGGAGTATGGGTGTGAGCTCTATAGACCATCCCTCTCTGCAATCAATAAACACTTGCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANOV13l, CG51622-10Protein SequenceSEQ ID NO: 392484 aaMW at 52426.8kDMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVIWLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQPDVLLNIITEIIHSILLPNQNGKLRSGVPVSLVKALGFEAAESSLSKDALVLTPASLWKPTSPVSQNOV13m,SNP13375774 ofSEQ ID NO: 393963 bpCG51622-01,ORF Start: ATG at 1ORF Stop: TAA at 961DNA SequenceSNP Pos: 248SNP Change: A to GATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAGTCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCGCATCACCCCATCCAGGCTGAAGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGGTAGGTGCTCTGCTCTCTCTCCCACTTTTTCCTTTACTACGGAGCTGGCCTCCAGACCCGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCTAANOV13m,SNP13375774 ofCG51622-01,SEQ ID NO: 394MW at 34552.3kDProtein SequenceSNP Pos: 83320 aaSNP Change: His to ArgMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKESLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKRITPSRLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHNTTEAQATIRMDTSASGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKGRCSALSPTFSFTTELASRPGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCPNOV13n,SNP1 3375775 ofSEQ ID NO: 395963 bpCG51622-01,ORF Start: ATG at 1ORF Stop: TAA at 961DNA SequenceSNP Pos: 250SNP Change: A to GATGGCCGGCCCGTGGACCTTCACCCTTCTCTGTGGTTTGCTGGCAGCCACCTTGATCCAAGCCACCCTCAGTCCCACTGCAGTTCTCATCCTCGGCCCAAAAGTCATCAAAGAAAGTCTGACACAGGAGCTGAAGGACCACAACGCCACCAGCATCCTGCAGCAGCTGCCGCTGCTCAGTGCCATGCGGGAAAAGCCAGCCGGAGGCATCCCTGTGCTGGGCAGCCTGGTGAACACCGTCCTGAAGCACGTCACCCCATCCAGGCTGTTGGTCATCACAGCTAACATCCTCCAGCTGCAGGTGAAGCCCTCGGCCAATGACCAGGAGCTGCTAGTCAAGATCCCCCTGGACATGGTGGCTGGATTCAACACGCCCCTGGTCAAGACCATCGTGGAGTTCCACATGACGACTGAGGCCCAAGCCACCATCCGCATGGACACCAGTGCAAGTGGCCCCACCCGCCTGGTCCTCAGTGACTGTGCCACCAGCCATGGGAGCCTGCGCATCCAACTGCTGCATAAGCTCTCCTTCCTGGTGAACGCCTTAGCTAAGCAGGTCATGAACCTCCTAGTGCCATCCCTGCCCAATCTAGTGAAAAACCAGCTGTGTCCCGTGATCGAGGCTTCCTTCAATGGCATGTATGCAGACCTCCTGCAGCTGGTGAAGGGTAGGTGCTCTGCTCTCTCTCCCACTTTTTCCTTTACTACGGAGCTGGCCTCCAGACCCGGAAAGGTGACCAAGTGGTTCAATAACTCTGCAGCTTCCCTGACAATGCCCACCCTGGACAACATCCCGTTCAGCCTCATCGTGAGTCAGGACGTGGTGAAAGCTGCAGTGGCTGCTGTGCTCTCTCCAGAAGAATTCATGGTCCTGTTGGACTCTGTGGTAAACCTCAGCACAAGGCAGAGAATAGGGCCGCCCAGGCCACATCATAGGAATTTCCTGAACACAGGGTGCCCCTAANOV13n,SNP13375775 ofCG51622-01,SEQ ID NO: 396MW at 3451 9.2kDProtein SequenceSNP Pos: 84320 aaSNP Change: Ile to ValMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPKVIKESLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVTPSRLKVITANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIPMDTSAsGPTRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVNNLLVPSLPNLVKNQLCPVIEASFNGMYADLLQLVKGRCSALSPTFSFTTELASRPGKVTKWFNNSAASLTMPTLDNIPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIGPPRPHHRNFLNTGCP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 13B.

TABLE 13BComparison of the NOV13 protein sequences.NOV13aMGVEDSQRRDPGRGLASCTCCPLTPGKMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13b---------------------------MAGPWTFTPLCGLLAATLIQATLSPTAVLILGPNOV13c---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13d---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13e-----------------------TGSTMAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13f------------------------------------------------TGSPTAVLILGPNOV13g---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13h---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13i---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13j---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13k---------------------------------------------------PTAVLILGPNOV13l---------------------------MAGPWTFTLLCGLLAATLIQATLSPTAVLILGPNOV13aKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHII--WLKVITNOV13bKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVI--WLKVITNOV13cKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVI--WLKVITNOV13dKVIKESLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHITPSRLKVITNOV13eKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVI--WLKVITNOV13fKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVI--WLKVITNOV13gKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHII--WLKVITNOV13hKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHII--WLKVITNOV13iKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVI--WLKVITNOV13jKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHII--WLKVITNOV13kKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHII--WLKVITNOV13lKVIKEKLTQELKDHNATSILQQLPLLSAMREKPAGGIPVLGSLVNTVLKHVI--WLKVITNOV13aANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13bANILQLQVKPSANDQELLVKIPLDMVAGFNT-----------------------------NOV13cANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13dANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13eANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13fANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13gANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13hANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13iANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13jANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13kANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13lANILQLQVKPSANDQELLVKIPLDMVAGFNTPLVKTIVEFHMTTEAQATIRMDTSASGPTNOV13aRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13b---------------------LSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13cRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKN-------------NOV13dRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13eRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13fRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13gRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13hRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13iRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13jRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13kRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13lRLVLSDCATSHGSLRIQLLHKLSFLVNALAKQVMNLLVPSLPNLVKNQLCPVIEASFNGMNOV13aYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13bYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13c-----QLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13dYADLLQLVKGRCSALSPTFSFTTELASRP------------G----KVTKWFNNSAASLTNOV13eYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13fYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13gYADLLQLVKGRCSALSPTFSFTTELASRP------------G----KVTKWFNNSAASLTNOV13hYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13iYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13jYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13kYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13lYADLLQLVKVPISLSIDRLEFDLLYPAIKGDTIQLYLGAKLLDSQGKVTKWFNNSAASLTNOV13aMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13bMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13cMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13dMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIG----NOV13eMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIG----NOV13fMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIG----NOV13gMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIG----NOV13hMPTLDNIPFSLIVSHPFSLIVSQDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13iMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVVNLSTRQRIG----NOV13jMPTLDNIPFSLVS---------QDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13kMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13lMPTLDNIPFSLIVS--------QDVVKAAVAAVLSPEEFMVLLDSVLPESAHRLKSSIGLNOV13aINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASNOV13bINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASNOV13cINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASNOV13d----------------------P--------------------P-----RP---------NOV13e----------------------P--------------------P-----RP---------NOV13f----------------------P--------------------P-----RP---------NOV13g----------------------P--------------------P-----RP---------NOV13hINEK-----------------------------------------------------EASNOV13i----------------------P--------------------P-----RP---------NOV13jINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASNOV13kINEKAADKLGPTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASNOV13lINEKAADKLGSTQIVKILTQDTPEFFIDQGHAKVAQLIVLEVFPSSEALRPLFTLGIEASNOV13aSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQCPFSLSQPDVLKNIITEIIHSILLPNOV13bSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQ-------PDVLKNIITEIIHSILLPNOV13cSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQ-------PDVLKNIITEIIHSILLPNOV13dHHRNFLNTGCP-------------------------------------------------NOV13eHHRNFLNTGCPLEG----------------------------------------------NOV13fHHRNFLNTGCPLE-----------------------------------------------NOV13gHHRNFLNTGCP-------------------------------------------------NOV13hSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQ-------PDVLKNIITEIIHSILLPNOV13iHHRNFLNTGCP-------------------------------------------------NOV13jSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQ-------PDVLKNIITEIIHSILLPNOV13kSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQ-------PDVLKNIITEIIHSILLPNOV13lSEAQFYTKGDQLILNLNNISSDRIQLMNSGIGWFQ-------PDVLKNIITEIIHSILLPNOV13aNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13bNQNGKLRSGVPVSLVKALGFEAAESSLSKDALVLTPASLWKPTSPVSQNOV13cNQNGKLRSGVPVSLVKALGFEAAESSLSKDALVLTPASLWKPTSPVSQNOV13d------------------------------------------------NOV13e------------------------------------------------NOV13f------------------------------------------------NOV13g------------------------------------------------NOV13hNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13i------------------------------------------------NOV13jNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13kNQNGKLRSGVPVSLVKALGFEAAESSLTKDALVLTPASLWKPSSPVSQNOV13lNQNGKLRSGVPVSLVKALGFEAAESSLSKDALVLTPASLWKPTSPVSQNOV13a(SEQ ID NO: 370)NOV13b(SEQ ID NO: 372)NOV13c(SEQ ID NO: 374)NOV13d(SEQ ID NO: 376)NOV13e(SEQ ID NO: 378)NOV13f(SEQ ID NO: 380)NOV13g(SEQ ID NO: 382)NOV13h(SEQ ID NO: 384)NOV13i(SEQ ID NO: 386)NOV13j(SEQ ID NO: 388)NOV13k(SEQ ID NO: 390)NOV13l(SEQ ID NO: 392)


Further analysis of the NOV13a protein yielded the following properties shown in Table 13C.

TABLE 13CProtein Sequence Properties NOV13aSignalP analysis:Cleavage site between residues 52 and 53PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 2; neg. chg 3H-region:length 2; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 1.17possible cleavage site: between 48 and 49>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 34-50−2.71PERIPHERALLikelihood = 1.16 (at 200)ALOM score: −2.71 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 41Charge difference: −0.5 C(1.5)-N(2.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 34)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):8.50Hyd Moment(95):5.94G content:1D/E content:2S/T content:0Score: −7.21Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.3%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: MGVEDSQPrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):30.4%: mitochondrial26.1%: cytoplasmic17.4%: Golgi 8.7%: endoplasmic reticulum 4.3%: vacuolar 4.3%: extracellular, including cell wall 4.3%: nuclear 4.3%: vesicles of secretory system>> prediction for CG51622-04 is mit (k = 23)


A search of the NOV13a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 13D.

TABLE 13DGeneseq Results for NOV13aNOV13aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAY77126Human neurotransmission-associated28 . . . 518484/491 (98%)0.0protein (NTAP) 2799056 - Homo 1 . . . 484484/491 (98%)sapiens, 484 aa. [WO200001821-A2,13-JAN-2000]AAG63976Amino acid sequence of a human28 . . . 518483/491 (98%)0.0Lng103 polypeptide - Homo sapiens, 1 . . . 484484/491 (98%)484 aa. [WO200161055-A2, 23-AUG-2001]ABU58539Human PRO polypeptide #140 - Homo28 . . . 518483/491 (98%)0.0sapiens, 484 aa. [US2003027272-A1, 1 . . . 484484/491 (98%)06-FEB-2003]ABU57070Human PRO polypeptide #140 - Homo28 . . . 518483/491 (98%)0.0sapiens, 484 aa. [US2003027280-A1, 1 . . . 484484/491 (98%)06-FEB-2003]ABU56075Human secreted/transmembrane28 . . . 518483/491 (98%)0.0protein, PRO1357 - Homo sapiens, 484 1 . . . 484484/491 (98%)aa. [US2003022298-A1, 30-JAN-2003]


In a BLAST search of public sequence databases, the NOV13a protein was found to have homology to the proteins shown in the BLASTP data in Table 13E.

TABLE 13EPublic BLASTP Results for NOV13aNOV13aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8TDL5Von Ebner minor protein - Homo28 . . . 518 484/491 (98%)0.0sapiens (Human), 484 aa.1 . . . 484484/491 (98%)Q96HK6Similar to DNA segment, Chr 2,28 . . . 518 481/491 (97%)0.0Massachusetts Institute of Technology1 . . . 484484/491 (97%)19 - Homo sapiens (Human), 484 aa.Q9BWZ6DJ1187J4.1.1 (Novel protein similar to227 . . . 518 285/292 (97%)e−154mouse von Ebner salivary gland1 . . . 285285/292 (97%)protein, isoform 1.) - Homo sapiens(Human), 285 aa (fragment).CAD13090Sequence 15 from Patent WO0174851 -28 . . . 337 276/310 (89%)e−146Homo sapiens (Human), 318 aa.1 . . . 294278/310 (89%)Q8SPF8Von Ebner minor salivary gland protein-28 . . . 508 269/481 (55%)e−144like protein - Bos taurus (Bovine), 473 aa.1 . . . 472353/481 (72%)


PFam analysis predicts that the NOV13a protein contains the domains shown in the Table 13F.

TABLE 13FDomain Analysis of NOV13aPfamNOV13aIdentities/ExpectDomainMatch RegionSimilaritiesValuefor theMatched Region


Example 14.

The NOV14 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 14A.

TABLE 14ANOV14 Sequence AnalysisNOV14a, CG51821-01SEQ ID NO: 3975103 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 5101ATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCAGCCGTTCTTCCCCCAGGCCAGGCCTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGGTGATCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAACGAGATTCACCTCCAAGTGAAGTGTGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGGCTCAAGGATGGGGTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCGTGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAATGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATACCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCACAGACCCGCCTCTCACTCCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGTCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTGTGAGCAGCCACCACGACAACCAACATTCACCACCAGGCTGGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGCCTCCTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCAAGAAGCACAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTACTCTATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCAGCTGCACCCATCAGCCTCCACGTGTCCTGTAAGGATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCTGACTTCGACGCTCAAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGGCAGCTGGTGAACCTGACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACAGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACCCTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTGTGCCCCTCAGGACGCTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTCCAGGTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATGTTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGAGTGGCGCGTGGTGGCAGAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCCCTGAACCTCAGCTGCCGCCTCCTGGGTGGCCCTGGGCCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTCTTCGTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCGCTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAATACATCTGTTCTGCCTCAAATGTCCTGGGCCCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATCCAGGGCGGGCATCGTCTTTCCATTTACTGCCTCTAGCTGGGTCTTCAAGGTGANOV14a, CG51821-01Protein SequenceSEQ ID NO: 3981700 aaMW at 180833.0kDMGFLPKLLLLASAVLPPGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTGDPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKCAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFVAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVTDPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHAIVARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLCEQPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSCKDAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQSCECAGVARGYRAGGAAGEPDLPCVDHSPGPAHLHMVPGWAAAPGCPLHPPAQRHSQGCHLLPLRCGPPWSGTPPLQTYHLGRPHAPRNLRLTYLLESHGGQLALvLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLCAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIAGRLQVEGEWRWAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGPASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENPGRASSFHLLPLAGSSRNOV14b, 229823091SEQ ID NO: 399717 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAGATCTTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTATGCCCCCAGGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGTCGACNOV14b, 229823091Protein SequenceSEQ ID NO: 400239 aaMw at 26882.1kDRSSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPRGVKILLSPSGRNILPVDNOV14c, 229823107SEQ ID NO: 401717 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAGATCTTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCATGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTATGCCCCCAGGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGTCGACNOV14c, 229823107Protein SequenceSEQ ID NO: 402239 aaMW at 26914.2kDRSSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFETSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSMANHRAQSEIHLQVKYAPRGVKILLSPSGRNILPVDNOV14d, 229823095SEQ ID NO: 403717 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAGATCTTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGTGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCATGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTATGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGTCGACNOV14d, 229823095Protein SequenceSEQ ID NO: 404239 aaMw at 26914.2kDRSSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSVDPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSMANHRAQSEIHLQVKYAPKGVKILLSPSGRNILPVDNOV14e, CG51821-02SEQ ID NO: 405717 bpDNA SequenceORF Start: at 7ORF Stop: at 712AGATCTTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTATGCCCCCAGGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGTCGACNOV14e, CG51821 -02Protein SequenceSEQ ID NO: 406235 aaMW at 26424.7kDSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPRGVKILLSPSGRNILPNOV14f, CG51821-03SEQ ID NO: 407717 bpDNA SequenceORF Start: at 7ORF Stop: at 712AGATCTTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGACCTTGGTCACAGTAACAGAGGAGCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCATGCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGTCGACNOV14f, CG51821-03Protein SequenceSEQ ID NO: 408235 aaMW at 26456.8kDSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSVDPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSMANHRAQSEIHLQVKYAPKGVKILLSPSGRNILPNOV14g, CG51821-04SEQ ID NO: 4095130 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 5128ATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCATTCTTCCCAGCAGGCCAGGCCTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAAATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGAGGAGCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTATGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCACTTCCATTAAGTGGCTCAAGGATGGGGTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCATGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAATGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATACCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCAACCACCCGCCTCTCACTCCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGCCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGGGGGCCGGGGCTGGACGGCGAGGCCTCCTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTACTCTATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGAGCCTGGGTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCATAACTTTGACACAAGCTGGGGCCTATCATTGCCAAGCCCAGGCCCCAGGCTCAGCCACCACGAGCCTAGCTGCACCCATCAGCCTCCACGTGTCCTATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGCGGCTGCTCCACGGGGATCGCCTTGTGGCCTCCACCCTACAAGGTGTGGGGGGACCCGAAGGCAGCTCTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCTGACTTCGACGCTCAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGGCAGCTGGTGAACCTGACCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACATGGTACCAGGATGGGCAGCAGCGCCTGGATGCCCACTCCAGCACCCCGCCTCTCCAGACCTATCACCTTGGACGTCCTCTACGCGCCCCGCAACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACAGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGGTGTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACCCTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTATGCCCCTCAGGACACTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTCCAGGTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATGTTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGCACGCGTGGTGGCAGAGCCTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTCTTCGTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCGCTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAATACATCTGTTCTGCCTCAAATGTCCTGGGCTCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATTCGGTGGAGATGGCTTTTCAGAAAGAGACCACGCAGCTCATTGATCCTGATGCAGCCACATGTGAGACCTCAACCTGTGCCCCACCCCTGGGCTGANOV14g, CG51821-04Protein SequenceSEQ ID NO: 4101709 aaMw at 182651.9kDMGFLPKLLLLASFFPAGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFMAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVNHPPLTPVLTAFLETQAGLVGILHCSVTSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANAARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLYPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPAPLQLLHKDRVVATSLPSGGGCSTCGGCSPRNKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSYAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQAVNVQVWPGATVREGQLVNLTCLVWTTHPAQLTYTWYQDGQQRLDAHSIPLPNVTVRDATSYRCGVGPPGRAPRLSRPITLDVLYAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEGVRVILAPEAAVPEGAPMMVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLYAPQDTVLSSFRDSPARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGARVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGSASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENSVEMAFQKETTQLIDPDAATCETSTCAPPLGNOV14h,SNP1 3382490SEQ ID NO: 4115103 bpof CG51821-01,ORF Start: ATG at 1ORF Stop: TGA at 5101DNA SequenceSNP Pos: 4056SNP Change: C to TATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCAGCCGTTCTTCCCCCAGGCCAGGCCTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGGTGATCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTGTGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCGGCAGCCTGGAGCGATGCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCGTGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAATGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATACCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCACAGACCCGCCTCTCACTCCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGTCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTGTGAGCAGCCACCACGACAACCAACATTCACCACCAGGCTGGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGCCTCCTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTACTCTATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGTGCCTGGGTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCATAACTTTGACACAAGCTGGGGCCTATCATTGCCAAGCCCAGGCCCCAGGCTCAGCCACCACGAGCCTAGCTGCACCCATCAGCCTCCACGTGTCCTGTAAGGATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGCGGCTGCTCCACGGGGATCGCCTTGTGGCCTCCACCCTACAAGGTGTGGGGGGACCCGAAGGCAGCTCCCCACGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCTGACTTCGACCCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACATGGTACCAGGATGGGCAGCAGCGCCTGGATGCCCACTCCATCCCCCTGCCCAACGTCACAGTCAGGGATGCCACCTCCTACCGCTGCGGTGTGGGCCCCCCTGGTCGGGCACCCCGCCTCTCCAGACCTATCACCTTGGACGTCCTCACGCGCCCCGCAACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACAGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACCCTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTGTGCCCCTCAGGATGCTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTCCAGGTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATGTTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGAGTGGCGGTGGCCCTGGGCCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTCTTCGTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCGCTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATCCAGGGCGGGCATCGTCTTTCCATTTACTGCCTCTAGCTGGGTCTTCAAGGTGANOV14h,SNP13382490 ofCG51821-01,SEQ ID NO: 412MW at 180833.0kDProtein SequenceSNP Pos: 13521700 aaSNP Change: Asp to AspMGFLPKLLLLASAVLPPGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTGDPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKCAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFVAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVTDPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANVARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLCEQPPRQPTFTTRLDLDAAGAGAGPRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSCKDAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDGPPWSGTPPLQTYHLGRPHAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLCAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGEWRVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGPASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENPGPASSFHLLPLAGSSRNOV14i,SNP1 3382489 ofSEQ ID NO: 4135103 bpCG51821-01,ORF Start: ATG at 1ORF Stop: TGA at 5101DNA SequenceSNP Pos: 4245SNP Change: G to AATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCAGCCGTTCTTCCCCCAGGCCAGGCCTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGGTGATCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTGTGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGGCTCAAGGATGGGGTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCGTGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAATGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATACCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCACAGACCCGCCTCTCACTCCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGTCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTGTGAGCAGCCACCACGACAACCAACATTCACCACCAGGCTGGACCTTCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTACTCTATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGTGCCTGGGTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCATACTaTTGACACAAGCTGGGGCCTATCATTGCCAAGCCCAGGCCCCAGGCTCAGCCACCACGAGCCTAGCTGCACCCATCAGCCTCCACGTGTCCTGTAAGGATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGCGGCTGCTCCACGGGGATCGCCTTGTGGCCTCCACCCTACAAGGTGTGGGGGGACCCGAAGGCAGCTCTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCTGACTTCGACGCTCAAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGGCAGCTGGTGAACCTGACCTGGATGCCCACTCCATCCCCCTGCCAACGTCACAGTCAGGGATGCCACCTCCTACCGCTGCGGTGTGGGCCCCCCTGGTCGGGCACCCCGCCTCTCCAGACCTATCACCTTGGACGTCCTCACGCGCCCCGCAACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACAGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACCCTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTGTGCCCCTCAGGACGCTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTCCAGGTGGCCCGAAACGCCCTACGGCTACAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATGTTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGAGTGGCGCGTGGTGGCAGAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCCCTGAACCTCAGCTGCCGCCTCCTGGGTGGCCCTGGGCCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCATGATGGTCTTCGTGGAGCCTGAGGGTGGCCTCCGGGGCATCCTGGATTGCCGAGTGGACAGCGAGCCGCTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAATACATCTGTTCTGCCTCAAATGTCCTGGGCCCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATCCAGGGCGGGCATCGTCTTTCCATTTACTGCCTCTAGCTGGGTCTTCAAGGTGANOV14i,SNP1 3382489 ofCG51821-01,SEQ ID NO: 414MW at 180833.0kDProtein SequenceSNP Pos: 14151700 aaSNP Change: Leu to LeuMGFLPKLLLLASAVLPPGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTGDPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKCAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFVAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATEADTGFYFCEVQNVHGSERSGPVSVVVTDPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANVARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPATLTVLCEQPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSCKDAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQSCECAGVARGYRAGGAAGEPDLPCVDHSPGPAHLHMVPGWAAAPGCPLHPPAQRHSQGCHLLPLRCGPPWSGTPPLQTYHLGRPHAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQTLCAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGEWRVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGPASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENPGRASSFHLLPLAGSSRNOV14j,SNP1 3377616 ofSEQ ID NO: 4155103 bpCG51821-01,ORF Start: ATG at 1ORF Stop: TGA at 5101DNA Sequence SNP Pos: 4768SNP Change: C to TATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCAGCCGTTCTTCCCCCAGGCCAGGCCTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTTCCCTGCCGACGTGGAGGTGCCCGACGGCATCACGGCCATCTGGTACTACGACTACTCGGGCCAGCGGGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGGTGATCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACTCAGAGCGAGATTCACCTCCAAGTGAAGTGTGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGGCTCAAGGATGGGGTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCGTGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAATGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAAGAACCATGTCCTGCTGGAGGATGCCCACTCCCATACCCTCCGGCTGCACTTGGCCACTAGGGCTGATACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCACAGACCCGCCTCTCACTCCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGTCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTGTGAGCAGCCACCACGACAACCAACATTCACCACCAGGCTGGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGCCTCCTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGACTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGTGCCTGGGTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCAGCTGCACCCATCAGCCTCCACGTGTCCTGTAAGGATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAGGGCTCAAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGGCAGCTGGTGAACCTGACCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACATGGTACCAGGATGGGCAGCAGCGCCTGGATGCCCACTCCATCCCCCTGCCCAACGTCACAGTCAGGGATGCCACCTCCTACCGCTGCGGTGTGGGCCCCCCTGGTCGGGCACCCCGCCTCTCCAGACCTATCACCTTGGACGTCCTCACGCGCCCCGCAACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACAGCCGCCCGCCCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGTGCGGGTGATCCTGGCTCCGGAGGCTGCCGTGCCTGAAGGTGCCCCCATCACAGTGACCTGTGCGGACCCTGCTGCCCACGCACCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTGTGCCCCTCAGGACGCTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGAGTGGCGCGTGGTGGCAGAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCCCTGAACCTCAGCTGCCGCCTCCTGGGTGGCCCTGGGCCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCGCTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGTCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAATACATCTGTTCTGCCTCAAATGTCCTGGGCCCTGCCTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATCCAGGGCGGGCATCGTCTTTCCATTTACTGCCTCTAGCTGGGTCTTCAAGGTGANOV14j,SNP13377616 ofCG51821-01,SEQ ID NO: 416MW at 180822.9kDProtein SequenceSNP Pos: 15901700 aaSNP Change: Pro to SerMGFLPKLLLLASAVLPPGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTGDPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKCAPKGVKILLSPSGPNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFVAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVTDPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANVARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLCEQPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAATLTQAGAYHCQAQAPGSATTSLAAPISLHVSCKDAPRHVTLTTLLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQSCECAGVARGYRAGGAAGEPDLPCVDHSPGPAHLHMVPGWAAAPGCPLHPPAQRHSQGCHLLPLRCGPPWSGTPPLQTYHLGRPHAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLCAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRIJQVEGEWRWAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSPLVASSQPQGAPAESHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGPASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENPGRASSFHLLPLAGSSRNOV14k, SNP13377617 ofSEQ ID NO: 4175103 bpCG51821-01,ORF Start: ATG at 1ORF Stop: TGA at 5101DNA SequenceSNP Pos: 4881SNP Change: C to TATGGGCTTCTTGCCCAAGCTTCTCCTCCTGGCCTCAGCCGTTCTTCCCCCAGGCCAGGCCTCATGGGGCGTCTCCAGTCCCCAGGACGTGCAGGGTGTGAAGGGGTCTTGCCTGCTTATCCCCTGCATCTTCAGCTCAGGTGGTGAGCCACTCGGCGGACCCCAAGCTGGTGGAGGCCCGCTTCCGCGGCCGCACCGAGTTCATGGGGAACCCCGAGCACAGGGTGTGCAACCTGCTGCTGAAGGACCTGCAGCCCGAGGACTCTGGTTCCTACAACTTCCGCTTCGAGATCAGTGAGGTCAACCGCTGGTCAGATGTGAAAGGCACCTTGGTCACAGTAACAGGTGATCCCAGGGTGCCCACCATTGCCTCCCCGGTGGAGCTTCTCGAGGGCACAGAGGTGGACTTCAACTGCTCCACTCCCTACGTATGCCTGCAGGAGCAGGTCAGACTGCAGTGGCAAGGCCAGGACCCTGCTCGCTCTGTCACCTTCAACAGCCAGAAGTTTGAGCCCACCGGCGTCGGCCACCTGGAGACCCTCCACATGGCCATGTCCTGGCAGGACCACGGCCGGATCCTGCGCTGCCAGCTCTCCGTGGCCAATCACAGGGCTCAGAGCGAGATTCACCTCCAAGTGAAGTGTGCCCCCAAGGGTGTGAAGATCCTCCTCAGCCCCTCGGGGAGGAACATCCTTCCAGGTGAGCTGGTCACACTCACCTGCCAGGTGAACAGCAGCTACCCTGCAGTCAGTTCCATTAAGTGGCTCAAGGATGGGGTACGCCTCCAAACCAAGACTGGTGTGCTGCACCTGCCCCAGGCAGCCTGGAGCGATGCTGGCGTCTACACCTGCCAAGCTGAGAACGGCGTGGGCTCTTTGGTCTCACCCCCCATCAGCCTCCACATCTTCGTGGCTGAGGTCCAGGTGAGCCCAGCAGGTCCCATCCTGGAGAACCAGACAGTGACACTAGTCTGCAACACACCCAATGAGGCACCCAGTGATCTCCGCTACAGCTGGTACAACTGGCTTCTACTTCTGTGAGGTGCAGAACGTCCATGGCAGCGAGCGCTCGGGCCCTGTCAGCGTGGTAGTCACAGACCCGCCTCTCACTCCAGTCCTGACAGCCTTCCTGGAGACCCAGGCGGGACTTGTGGGCATCCTTCACTGCTCTGTGGTCAGTGAGCCCCTGGCCACACTGGTGCTGTCACATGGGGGTCATATCCTGGCCTCCACCTCCGGGGACAGTGATCACAGCCCACGCTTCAGTGGTACCTCTGGTCCCAACTCCCTGCGCCTGGAGATCCGAGACCTGGAGGAAACTGACAGTGGGGAGTACAAGTGCTCAGCCACCAACTCCCTTGGAAATGCAACCTCCACCCTGGACTTCCATGCCAATGTCGCCCGTCTCCTCATCAGCCCGGCAGCCGAGGTGGTGGAAGGACAGGCAGTGACACTGAGCTGCAGAAGCGGCCTAAGCCCCACACCTGATGCCCGCTTCTCCTGGTACCTGAATGGAGCCCTGCTTCACGAGGGTCCCGGCAGCAGCCTCCTGCTCCCCGCGGCCTCCAGCACTGACGCCGGCTCATACCACTGCCGGGCCCGGGACGGCCACAGTGCCAGTGGCCCCTCTTCGCCAGCTGTTCTCACTGTGCTCTGTGAGCAGCCACCACGACAACCAACATTCACCACCAGGCTGGACCTTGATGCCGCTGGGGCCGGGGCTGGACGGCGAGGCCTCCTTTTGTGCCGTGTGGACAGCGACCCCCCCGCCAGGCTGCAGCTGCTCCACAAGGACCGTGTTGTGGCCACTTCCCTGCCATCAGGGGGTGGCTGCAGCACCTGTGGGGGCTGTTCCCCACGCATGAAGGTCACCAAAGCCCCCAACTTGCTGCGTGTGGAGATTCACAACCCTTTGCTGGAAGAGGAGGGCTTGTACCTCTGTGAGGCCAGCAATGCCCTGGGCAACGCCTCCACCTCAGCCACCTTCAATGGCCAGGCCACTGTCCTGGCCATTGCACCATCACACACACTTCAGGAGGGCACAGAAGCCAACTTGACTTGCAACGTGAGCCGGGAAGCTGCTGGCAGCCCTGCTAACTTCTCCTGGTTCCGAAATGGGGTGCTGTGGGCCCAGGGTCCCCTGGAGACCGTGACACTGCTGCCCGTGGCCAGAACTGATGCTGCCCTTTACGCCTGCCGCATCCTGACTGAGGCTGGTGCCCAGCTCTCCACTCCCGTGCTCCTGAGTGTACTCTATCCCCCGGACCGTCCAAAGCTGTCAGCCCTCCTAGACATGGGCCAGGGCCACATGGCTCTGTTCATCTGCACTGTGGACAGCCGCCCCCTGGCCTTGCTGGCCTTGTTCCATGGGGAGCACCTCCTGGCCACCAGCCTGGGTCCCCAGGTCCCATCCCATGGTCGGTTCCAGGCTAAAGCTGAGGCCAACTCCCTGAAGTTAGAGGTCCGAGAACTGGGCCTTGGGGACTCTGGCAGCTACCGCTGTGAGGCCACAAATGTTCTTGGATCATCCAACACCTCACTCTTCTTCCAGGTCCGAGGTGCCTGGGTCCAGGTGTCACCATCACCTGAGCTCCAAGAGGGCCAGGCTGTGGTCCTGAGCTGCCAGGTACACACAGGAGTCCCAGAGGGGACCTCATATCGTTGGTATCGGGATGGCCAGCCCCTCCAGGAGTCGACCTCGGCCACGCTCCGCTTTGCAGCCATAACTTTGACACAAGCTGGGGCCTATCATTGCCAAGCCCAGGCCCCAGGCTCAGCCACCACGAGCCTAGCTGCACCCATCAGCCTCCACGTGTCCTGTAAGGATGCCCCACGCCACGTCACACTCACTACCCTGATGGACACAGGCCCTGGACGACTGGGCCTCCTCCTGTGCCGTGTGGACAGTGACCCTCCGGCCCAGCTGCGGCTGCTCCACGGGGATCGCCTTGTGGCCTCCACCCTACAAGGTGTGGGGGGACCCGAAGGCAGCTCTCCCAGGCTGCATGTGGCTGTGGCCCCCAACACACTGCGTCTGGAGATCCACGGGGCTATGCTGGAGGATGAGGGTGTCTATATCTGTGAGGCCTCCAACACCCTGGGCCAGGCCTCGGCCTCAGCTGACTTCGACGCTCAAAGCTGTGAATGTGCAGGTGTGGCCCGGGGCTACCGTGCGGGAGGGGCAGCTGGTGAACCTGACCTGCCTTGTGTGGACCACTCACCCGGCCCAGCTCACCTACACATGGTACCAGGATGGGCAGCAGCGCGGGCCCCCCTGGTCGGGCACCCCGCCTCTCCAGACCTATCACCTTGGACGTCCTCACGCGCCCCGCAACCTGCGCCTGACCTACCTCCTGGAGAGCCATGGCGGGCAGCTGGCCCTGGTACTGTGCACTGTGGACACCGCCCGCACCGCCCAGCTGGCCCTCAGCCACGCCGGTCGCCTCTTGGCCTCCTCGACAGCAGCCTCTGTCCCCAACACCCTGCGCCTGGAGCTGCGAGGGCCACAGCCCAGGGATGAGGGTTTCTACAGCTGCTCTGCCCGCAGCCCTCTGGGCCAGGCCAACACGTCCCTGGAGCTGCGGCTGGAGGTGCGGGTGATCCTGGCCCACACTCTATACTTGGTACCACAACGGTCGTTGGCTGCAGGAGGGTCCAGCTGCCTCACTCTCATTCCTGGTGGCCACGCGGGCTCATGCAGGCGCCTACTCTTGCCAGGCCCAGGATGCCCAGGGCACCCGCAGCTCCCGTCCTGCTGCCCTGCAAGTCCTCTGTGCCCCTCAGGACGCTGTCCTGTCCTCCTTCCGGGACTCCAGGGCCAGATCCATGGCTGTGATACAGTGCACTGTGGACAGTGAGCCACCTGCTGAGCTGGCCCTATCTCATGATGGCAAGGTGCTGGCCACGAGCAGCGGGGTCCACAGCTTGGCATCAGGGACAGGCCATGTCCAGGTGGCCCGAAACGCCCTACGGCTGCAGGTGCAAGATGTGCCTGCAGGTGATGACACCTATGTTTGCACAGCCCAAAACTTGCTGGGCTCAATCAGCACCATCGGGCGGTTGCAGGTAGAAGGTGAGTGGCGCGTGGTGGCAGAGCCTGGCCTGGACGTGCCTGAGGGCGCTGCCCTGAACCTCAGCTGCCGCCTCCTGGGTGGCCCTGGGCCTGTGGGCAACTCCACCTTTGCATGGTTCTGGAATGACCGGCGGCTGCACGCGGAGCCTGTGCCCACTCTCGCCTTCACCCACGTGGCTCGTGCTCAAGCTGGGATGTACCACTGCCTGGCTGAGCTCCCCACTGGGGCTGCTGCCTCTGCTCCAGTCATGCTCCGTGTGCTCTACCCTCCCAAGACGCCCACCGCTCGCCAGCCTGACTCTCCACCTTGGCAGTCGACTGGTGGCCTCCAGTCAGCCCCAGGGTGCTCCTGCAGAGCCACACATCCATGTCCTGGCTTCCCCCAATGCCCTGAGGGTGGACATCGAGGCGCTGAGGCCCAGCGACCAAGGGGAATACATCTGTTCTGCCTCAAATGTCCTGGGCCCTGCTTCTACCTCCACCTACTTTGGGGTCAGAGCCCTGCACCGCCTGCATCAGTTCCAGCAGCTGCTCTGGGTCCTGGGACTGCTGGTGGGCCTCCTGCTCCTGCTGTTGGGCCTGGGGGCCTGCTACACCTGGAGAAGGAGGCGTGTTTGTAAGCAGAGCATGGGCGAGAATCCAGGGCGGGCATCGTCTTTCCATTTACTGCCTCTAGCTGGGTCTTCAAGGTGANOV14k,SNP1 3377617 ofCG51821-01,SEQ ID NO: 4181MW at 180833.0kDProtein SequenceSNP Pos: 16271700 aaSNP Change: Ala to AlaMGFLPKLLLLASAVLPPGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTGDPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKCAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFVAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVTDPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHANVARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCPARDGHSASGPSSPAVLTVLCEQPPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSLAAPISLHVSCKDAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGANLEDEGVYICEASNTLGQASASADFDAQSCECAGVARGYRAGGAAGEPDLPCVDHSPGPAHLHMVPGWAAAPGCPLHPPAQRHSQGCHLLPLRCGPPWSGTPPLQTYHLGRPHAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAGAYSCQAQDAQGTRSSRPAALQVLCAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELALSHDGKVLATSSGVHSLASGTGHVQVAPNALRLQVQDVPAGDDTYVCTAQNLLGSISTIGRLQVEGEWRVVAEPGLDVPEAGAALNLSCRLLGPGPVGNSTFAWFWNDRRLHAEPVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYICSASNVLGPASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKQSMGENPGRASSFHLLPTAGSSR


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 14B.

TABLE 14BComparison of the NOV14 protein sequences.NOV14aMGFLPKLLLLASAVLPPGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14b------------------RSSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14c------------------RSSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14d------------------RSSWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14e--------------------SWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14f--------------------SWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14gMGFLPKLLLLAS-FFPAGQASWGVSSPQDVQGVKGSCLLIPCIFSFPADVEVPDGITAIWNOV14aYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14bYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14cYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14dYYDYSGQRQVVSHSVDPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14eYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14fYYDYSGQRQVVSHSVDPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14gYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLLKDLQPEDSGSYNFRFEINOV14aSEVNRWSDVKGTLVTVTGDPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14bSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14cSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14dSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14eSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14fSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14gSEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNCSTPYVCLQEQVRLQWQGQDNOV14aPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKCAPKNOV14bPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPRNOV14cPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSMANHRAQSEIHLQVKYAPRNOV14dPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSMANHRAQSEIHLQVKYAPKNOV14ePARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPRNOV14fPARSVTFNSQKFEPTGVGHLETLHNAMSWQDHGRILRCQLSMANHRAQSEIHLQVKYAPKNOV14gPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQLSVANHRAQSEIHLQVKYAPKNOV14aGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDNOV14bGVKILLSPSGRNILPVD-------------------------------------------NOV14cGVKILLSPSGRNILPVD-------------------------------------------NOV14dGVKILLSPSGRNILPVD-------------------------------------------NOV14eGVKILLSPSGRNILP---------------------------------------------NOV14fGVKILLSPSGRNILP---------------------------------------------NOV14gGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKWLKDGVRLQTKTGVLHLPQAAWSDNOV14aAGVYTCQAENGVGSLVSPPISLHIFVAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gAGVYTCQAENGVGSLVSPPISLHIFMAEVQVSPAGPILENQTVTLVCNTPNEAPSDLRYSNOV14aWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVTDPPLTPVLTANOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEVQNVHGSERSGPVSVVVNHPPLTPVLTANOV14aFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gFLETQAGLVGILHCSVVSEPLATLVLSHGGHILASTSGDSDHSPRFSGTSGPNSLRLEIRNOV14aDLEETDSGEYKCSATNSLGNATSTLDFHANAARLLISPAAEVVEGQAVTLSCRSGLSPTPNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gDLEETDSGEYKCSATNSLGNATSTLDFHANAARLLISPAAEVVEGQAVTLSCRSGLSPTPNOV14aDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLCEQPNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gDARFSWYLNGALLHEGPGSSLLLPAASSTDAGSYHCRARDGHSASGPSSPAVLTVLY--PNOV14aPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gPRQPTFTTRLDLDAAGAGAGRRGLLLCRVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGNOV14aGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gGCSPRMKVTKAPNLLRVEIHNPLLEEEGLYLCEASNALGNASTSATFNGQATVLAIAPSHNOV14aTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gTLQEGTEANLTCNVSREAAGSPANFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILNOV14aTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLANOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gTEAGAQLSTPVLLSVLYPPDRPKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLANOV14aTSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gTSLGPQVPSHGRFQAKAEANSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWNOV14aVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGANOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gVQVSPSPELQEGQAVVLSCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGANOV14aYHCQAQAPGSATTSLAAPISLBVSCKDAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gYHCQAQAPGSATTSLAAPISLHVSY--APRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLNOV14aRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gRLLHGDRLVASTLQGVGGPEGSSPRLHVAVAPNTLRLETHGAMLEDEGVYICEASNTLGQNOV14aASASADFDAQSCECAGVARGYRAGGAAGEPDLPCVDHSPGPAHLHMVPGWAAAPGCPLHPNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gASASADFDAQAVNVQVWPGATVREGQLVN--LTCLVWTTHPAQLTYTWYQDGQQRLDAHSNOV14aPAQRHSQGCHLLPLRCG--PPWSGTPPLQTYHLGRPHAPRNLRLTYLLESHGGQLALVLCNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gIPLPNVTVRDATSYRCGVGPPGRAPRLSRPITLDVLYAPRNLRLTYLLESHGGQLALVLCNOV14aTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gTVDSRPPAQLALSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSNOV14aLELRLE-VRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gLELRLEGVRVILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVNOV14aATRAHAGAYSCQAQDAQGTRSSRPAALQVLCAPQDAVLSSFRDSRARSMAVIQCTVDSEPNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gATPAHAGAYSCQAQDAQGTRSSRPAALQVLYAPQDAVLSSFRDSRARSMAVIQCTVDSEPNOV14aPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gPAELALSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSNOV14aISTIGRLQVEGEWRVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gISTIGRLQVEGAR-VVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPNOV14aVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGILNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gVPTLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTNMVFVEPEGGLRGILNOV14aDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYINOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gDCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGEYINOV14aCSASNVLGPASTSTYFGVPALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKNOV14b------------------------------------------------------------NOV14c------------------------------------------------------------NOV14d------------------------------------------------------------NOV14e------------------------------------------------------------NOV14f------------------------------------------------------------NOV14gCSASNVLGSASTSTYFGVPALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRRRVCKNOV14aQSMGENP-----GEASSFHLLPLAGSSR---------NOV14b-------------------------------------NOV14c-------------------------------------NOV14d-------------------------------------NOV14e-------------------------------------NOV14f-------------------------------------NOV14gQSMGENSVEMAFQKETTQLIDPDAATCETSTCAPPLGNOV14a(SEQ ID NO: 398)NOV14b(SEQ ID NO: 400)NOV14c(SEQ ID NO: 402)NOV14d(SEQ ID NO: 404)NOV14e(SEQ ID NO: 406)NOV14f(SEQ ID NO: 408)NOV14g(SEQ ID NO: 410)


Further analysis of the NOV14a protein yielded the following properties shown in Table 14C.

TABLE 14CProtein Sequence Properties NOV14a SignalP analysis:Cleavage site between residues 21 and 22PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos. chg 1; neg. chg 0H-region:length 22; peak value 9.20PSG score:4.80GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 2.74possible cleavage site: between 20 and 21>>> Seems to have a cleavable signal peptide (1 to 20)ALOM:Klein et al's method for TM region allocationInit position for calculation: 21Tentative number of TMS(s) for the threshold 0.5: 3Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane−11.251647-1663PERIPHERALLikelihood =0.69 (at 36)ALOM score: −11.25 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 10Charge difference: −2.0 C(0.0)-N(2.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 1664 to 1700)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):6.81Hyd Moment(95):7.54G content:3D/E content:1S/T content:4Score: −4.95Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: RRRR (5) at 1671pat7: nonebipartite: nonecontent of basic residues: 7.3%NLS Score: −0.16KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: 1667Dileucine motif in the tail: foundLL at 1692checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):33.3%: endoplasmic reticulum22.2%: Golgi22.2%: extracellular, including cell wall11.1%: plasma membrane11.1%: vesicles of secretory system>> prediction for CG51821-01 is end (k = 9)


A search of the NOV14a protein against the Geneseq database, a proprietary database that contains sequences published in. patents and patent publication, yielded several homologous proteins shown in Table 14D.

TABLE 14DGeneseq Results for NOV14a NOV14aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABB05044Human NOV6 protein SEQ ID NO: 24 -1 . . . 17001700/1700 (100%) 0.0Homo sapiens, 1700 aa.1 . . . 17001700/1700 (100%) [WO200190155-A2, 29-NOV-2001]AAE09448Human sbg24878Sla protein #1 -1 . . . 16831588/1695 (93%)0.0Homo sapiens, 1709 aa.1 . . . 16721598/1695 (93%)[WO200160850-A1, 23-AUG-2001]ME09449Human sbg24878Sla protein #2 -1 . . . 16711576/1683 (93%)0.0Homo sapiens, 1694 aa.1 . . . 16601586/1683 (93%)[WO200160850-A1, 23-AUG-2001]ABG10466Novel human diagnostic protein18 . . . 1671 1562/1666 (93%)0.0#10457 - Homo sapiens, 1839 aa.162 . . . 1805 1573/1666 (93%)[WO200175067-A2, 11-OCT-2001]ABP69461Human polypeptide SEQ ID NO482 . . . 1683 1118/1214 (92%)0.01508 - Homo sapiens, 1263 aa.35 . . . 1232 1126/1214 (92%)[WO200270539-A2, 12-SEP-2002]


In a BLAST search of public sequence databases, the NOV14a protein was found to have homology to the proteins shown in the BLASTP data in Table 14E.

TABLE 14EPublic BLASTP Results for NOV14aNOV14aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9BZZ2Sialoadhesin precursor (Sialic acid 1 . . . 16831594/1695(94%)0.0binding Ig-like lectin-1) (Siglec- 1) 1 . . . 16781603/1695(94%)(CD169 antigen) - Homo sapiens(Human), 1709 aa.Q62230Sialoadhesin precursor (Sialic acid 8 . . . 16831167/1688(69%)0.0binding Ig-like lectin-1) (Siglec- 1) 7 . . . 16751333/1688(78%)(Sheep erythrocyte receptor) (SER) -Mus musculus (Mouse), 1694 aa.BAC56927FLJ00411 protein - Homo sapiens798 . . . 1091 288/294(97%) e−162(Human), 437 aa (fragment).17 . . . 308 289/294(97%)P35329B-cell receptor CD22 precursor (Leu-22 . . . 634 167/649(25%)5e−3714) (B-lymphocyte cell adhesion26 . . . 638 274/649(41%)molecule) (BL-CAM) (Siglec-2) - Musmusculus (Mouse), 862 aa.A46512CD22 homolog/B lymphocyte-22 . . . 634 163/653(24%)6e−35restricted adhesion molecule - mouse,26 . . . 644 271/653(40%)868 aa.


PFam analysis predicts that the NOV14a protein contains the domains shown in the Table 14F.

TABLE 14FDomain Analysis of NOV14aIdentities/SimilaritiesPfamNOV14afor theExpectDomainMatch RegionMatched RegionValueig256 . . . 30820/54 (37%)1.5e−1143/54 (80%)ig340 . . . 39317/55 (31%)4.3e−1044/55 (80%)ig427 . . . 49417/71 (24%)0.008251/71 (72%)ig525 . . . 57816/55 (29%)2.1e−0639/55 (71%)ig620 . . . 69412/78 (15%)0.9253/78 (68%)ig725 . . . 77915/56 (27%)0.02735/56 (62%)ig813 . . . 88113/72 (18%)0.02247/72 (65%)ig912 . . . 96516/55 (29%)2.1e−0739/55 (71%)ig1003 . . . 107414/75 (19%)0.02352/75 (69%)ig1191 . . . 124813/61 (21%)0.004840/61 (66%)ig1278 . . . 133015/55 (27%)0.1634/55 (62%)ig1364 . . . 143113/72 (18%)0.0008450/72 (69%)


Example 15

The NOV15 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 15A.

TABLE 15ANOV15 Sequence AnalysisNOV15a, CG51992-05SEQ ID NO: 4191511 bpDNA SequenceORF Start: ATG at 339ORF Stop: TGA at 1203AGAGAAGGAGCTAAGAAGCCTGTGGCTACTGAGAAGAGGCCAGCTAAGTGATGCTGGCATGTAGGAAGCAGGCACACTACTTGTGGATGGGGAGCATGGTGACTGGAGAGACCAGTGAGGGCAATTTGCATCCTTTGAGGGCAATTTGGTCTCCAAAGAACAGAGTGATCTGGAGGGAGTGACAGCCAAGAGTCAGCCAGAGCATCTCAGCCATCCCAGGAATCAGAGAAATCCAGGGAAGCCAGTCTTAATATGATGGAAACATCTCTGAACTTCTAAAAGACCAAGGTTGGCGTTTTAGCTCTATTAATTTTACTTCGTCTTGGCCAGAATTCACAATGACAACAGTGACAGTGACCACAGAAATTCCCCCAAGGGATAAGATGGAAGATAATTCTGCCTTGTATGAGTCTACGTCCGCTCACATTATTGAAGAAACCGAGTATGTGAAAAAGATTCGAACTACTCTGCAAAAGATCAGGACCCAGATGTTTAAAGATGAAATAAGACATGACAGTACAAATCACAAACTAGATGCAAAGCTGCAAGCCTCCAGAGAAGCAGGAGCAGCAGCTCTGAGAAACGTGGCCCAGAGATTATTTGAAAACTACCAAACGCAATCTGAAGAAGTGAGAAAGAAGCAGGAGGACAGTAAACAATTACTCCAGGTTAACAAGCTTGAAAAAGAACAGAAATTGAAACAACATGTTGAAAATCTGAATCAAGTTGCTGAAAAACTTGAAGAAAAACACAGTCAAATTACAGAATTGGAGAACCTTGTACAGAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAAGGTATCTAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACAGCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAAATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTTCTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAATCTTGTTTTCAGATTTTTTTTTCTACTGGNOV15a, CG51992-05SEQ ID NO: 420288 aaMW at 33581.0kDProtein SequenceMTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSTNHKLDAKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRKNOV15b, CG51992-01SEQ ID NO: 421705 bpDNA SequenceORF Start: at 3ORF Stop: TGA at 423TACAAAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAAGGTATCTAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACACCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAAATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTTCTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAAAAANOV15b, CG51992-01SEQ ID NO: 422140 aaMW at 16324.7kDProtein SequenceQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRKNOV15c, CG51992-02SEQ ID NO: 4231080 bpDNA SequenceORF Start: ATG at 45ORF Stop: TGA at 798GTTTAAAGATGAAATGAGACATGACAGTACAAATCACAAACTAGATGCAAAGTTTGGATTTGCTTATGAAAAAGATAAAAGGAAAAGACCTACAGCTCTTAGAAATGAACAAAGAGAATGAAGTATTGAAAATCAAGCTGCAAGCCTCCAGAGAAGCAGGAGCAGCAGCTCTGAGAAACGTGGCCCAGAGATTATTTGAAAACTACCAAACGCAATCTGAAGAAGTGAGAAAGAAGCAGGAGGGCAGTAAACAATTACTCCAGGTTAACAAGCTTGAAAAAGAACAGAAATTGAAACAACATGTTGAAAATCTGAATCAAGTTGCTGAAAAACTTGAAGAAAAACACAGTCAAATTACAGAATTGGAGAACCTTGTACAGAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAAGGTATCTAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACAGCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAAATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTTCTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAAAAANOV15c, CG51992-02SEQ ID NO: 424251 aaMW at 29229.5kDProtein SequenceMQSLDLLMKKIKGKDLQLLEMNKENEVLKIKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEGSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRKNOV15d, CG51992-03SEQ ID NO: 4251442 bpDNA SequenceORF Start: ATG at 155ORF Stop: TGA at 1160GCCCAGGGGAGGAGCAGCACCGGGACCCCGCGTCGGCTGGGCGCCCCACAAGGGAAGCCAGTCTTAATATGATGGAAACATCTCTGAACTTCTAAAAGACCAAGGTTGGCGTTTTAGCTCTATTAATTTTACTTCGTCTTGGCCAGAATTCACAATGACAACAGTGACAGTGACCACAGAAATTCCCCCAAGGGATAAGATGGAAGATAATTCTGCCTTGTATGAGTCTACGTCCGCTCACATTATTGAAGAAACCGAGTATGTGAAAAAGATTCGAACTACTCTGCAAAAGATCAGGACCCAGATGTTTAAAGATGAAATAAGACATGACAGTACAAATCACAAACTAGATGCAAAGCACTGTGGAAACCTTCAACAGGGCTCTGATTCTGAAATGGATCCTTCTTGTTGCAGTTTGGATTTGCTTATGAAAAAGATAAAAGGAAAAGACCTACAGCTCTTAGAAATGAACAAAGAGAATGAAGTATTGAAAATCAAGCTGCAAGCCTCCAGAGAAGCAGGAGCAGCAGCTCTGAGAAACGTGGCCCAGAGATTATTTGAAAACTACCAAACGCAATCTGAAGAAGTGAGAAAGAAGCAGGAGGACAGTAAACAATTACTCCAGGTTAACAAGCTTGAAAAAGAACAGAAATTGAAACAACATGTTGAAAATCTGAATCAAGTTGCTGAAAAACTTGAAGAAAAACACAGTCAAATTACAGAATTGGAGAACCTTGTACAGAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAAGGTATCTAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACAGCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAAATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTTCTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAAAAANOV15d, CG51992-03SEQ ID NO: 426335 aaMW at 38869.2kDProtein SequenceMTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSTNHKLDAKHCGNLQQGSDSEMDPSCCSLDLLMKKIKGKDLQLLENNKENEVLKIKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRKNOV15e, CG51992-04SEQ ID NO: 4271482bpDNA SequenceORF Start: ATG at 195ORF Stop: TGA at 1200GTGTGTGGGTGTCCAGGTGCCTTTCCAGCGGCTTCCCCAGTGGAGTTCCTGGCATCAAGGACATTTCCTGTAAAAGGGTCCTTGTTGAAGAGGGAAGCCAGTCTTAATATGATGGAAACATCTCTGAACTTCTAAAAGACCAAGGTTGGCGTTTTAGCTCTATTAATTTTACTTCGTCTTGGCCAGAATTCACAATGACAACAGTGGCAGTGACCACAGAAATTCCCCCAAGGGATAAGATGGAAGATAATTCTGCCTTGTATGAGTCTACGTCCGCTCACATTATTGAAGAAACCGAGTATGTGAAAAAGATTCGAACTACTCTGCAAAAGATCAGGACCCAGATGTTTAAAGATGAAATAAGACATGACAGTACAAATCACAAACTAGATGCAAAGCACTGTGGAAACCTTCAACAGGGCTCTGATTCTGAAATGGATCCTTCTTGTTGCAGTTTGGATTTGCTTATGAAAAAGATAAAAGGAAAAGACCTACAGCTCTTAGAAATGAACAAAGAGAATGAAGTATTGAAAATCAAGCTGCAAGCCTCCAGAGAAGCAGGAGCAGCAGCTCTGAGAAACGTGGCCCAGAGATTATTTGAAAACTACCAAACGCAATCTGAAGAAGTGAGAAAGAAGCAGGAGGACAGTAAACAATTACTCCAGGTTAACAAGCTTGAAAAAGAACAGAAATTGAAACAACATGTTGAAAATCTGAATCAAGTTGCTGAAAAACTTGAAGAAAAACACAGTCAAATTACAGAATTGGAGAACCTTGTACAGAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAAGGTATCTAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACAGCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAAATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTT+E,UNA CTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAAAAA NOV15e, CG51992-04SEQ ID NO: 428335 aaMW at 38839.1kDProtein SequenceMTTVAVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSTNHKLDAKHCGNLQQGSDSEMDPSCCSLDLLMKKIKGKDLQLLEMNKENEVLKIKLQASREAGAAALRINAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRKNOV15f, SNP13382486SEQ ID NO: 4291511 bpof CG51992-05,ORF Start: ATG at 339ORF Stop: TGA at 1203DNA SequenceSNP Pos: 506SNP Change: A to GAGAGAAGGAGCTAAGAAGCCTGTGGCTACTGAGAAGAGGCCAGCTAAGTGATGCTGGCATGTAGGAAGCAGGCACACTACTTGTGGATGGGGAGCATGGTGACTGGAGAGACCAGTGAGGGCAATTTGCATCCTTTGAGGGCAATTTGGTCTCCAAAGAACAGAGTGATCTGGAGGGAGTGACAGCCAAGAGTCAGCCAGAGCATCTCAGCCATCCCAGGAATCAGAGAAATCCAGGGAAGCCAGTCTTAATATGATGGAAACATCTCTGAACTTCTAAAAGACCAAGGTTGGCGTTTTAGCTCTATTAATTTTACTTCGTCTTGGCCAGAATTCACAATGACAACAGTGACAGTGACCACAGAAATTCCCCCAAGGGATAAGATGGAAGATAATTCTGCCTTGTATGAGTCTACGTCCGCTCACATTATTGAAAGAAACCGAGTATGTGAAAAAGATTCGAACTACTCTGCAAAGATCAGGACCCAGATGTTTAAAGATGAAATGAGACATGACAGTACAAATCACAAACTAGATGCAAAGCTGCAAGCCTCCAGAGAAGCAGGAGCAGCAGCTCTGAGAAACGTGGCCCAGAGATTATTTGAAAACTACCAAACGCAATCTGAAGAAGTGAGAAAGAAGCAGGAGGACAGTAAACAATTACTCCAGGTTAACAAGCTTGAAAAAGAACAGAAATTGAAACAACATGTTGAAAATCTGAATCAAGTTGCTGAAAAACTTGAAGAAAAACACAGTCAAATTACAGAATTGGAGAACCTTGTACAGAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAAGGTATCTAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACAGCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAAATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTTCTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAATCTTGTTTTCAGATTTTTTTTTCTACTGGNOV15f, SNP13382486SEQ ID NO: 430288 aaMW at 33599.1kDof CG51992-05,SNP Pos: 56SNP Change:Protein SequenceIle to MetMTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEMRHDSTNHKLDAKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRKNOV15g, SNP13382487SEQ ID NO: 4311511 bpof CG51992-05,ORF Start: ATG at 339ORF Stop: TGA at 1203DNA SequenceSNP Pos: 1360SNP Change: A to GAGAGAAGGAGCTAAGAAGCCTGTGGCTACTGAGAAGAGGCCAGCTAAGTGATGCTGGCATGTAGGAACCAGGCACACTACTTGTGGATGGGGAGCATGGTGACTGGAGAGACCAGTGAGGGCAATTTGCATCCTTTGAGGGCAATTTGGTCTCCAAAGAACAGAGTGATCTGGAGGGAGTGACAGCCAAGAGTCAGCCAGAGCATCTCAGCCATCCCAGGAATCAGAGAAATCCAGGGAAGCCAGTCTTAATATGATGGAAACATCTCTGAACTTCTAAAAGACCAAGGTTGGCGTTTTAGCTCTATTAATTTTACTTCGTCTTGGCCAGAATTCACAATGACAACAGTGACAGTGACCACAGAAATTCCCCCAAGGGATAAGATGGAAGATAATTCTGCCTTGTATGAGTCTACGTCCGCTCACATTATTGAAGAAACCGAGTATGTGAAAAAGATTCGAACTACTCTGCAAAAGATCAGGACCCAGATGTTTAAAGATGAAATAAGACATGACAGTACAAATCACAAACTAGATGCAAAGCTGCAAGCCTCCAGAGAAGCAGGAGCAGCAGCTCTGAGAAACGTGGCCCAGAGATTATTTGAAAACTACCAAACGCAATCTGAAGAAGTGAGAAAGAAGCAGGAGGACAGTAAACAATTACTCCAGGTTAACAAGCTTGAAAAAGAACAGAAATTGAAACAACATGTTGAAAATCTGAATCAAGTTGCTGAAAAACTTGAAGAAAAACACAGTCAAATTACAGAATTGGAGAACCTTGTACAGAGAATGGAAAAGGAAAAGAGAACACTACTAGAAAGAAAACTGTCTTTGGAAAACAAGCTACTGCAACTCAAATCCAGTGCTACATATGGAAAAAGTTGCCAGGATCTTCAGAGGGAGATTTCCATTCTCCAGGAGCAGATCTCTCATCTGCAGTTTGTGATTCACTCCCAACATCAGAACCTGCGCAGTGTCATCCAGGAGATGGAAGGATTAAAAAATAATTTAAAAGAACAAGACAAAAGAATTGAAAATCTCAGAGAAAAGGTTAACATACTTGAAGCCCAGAATAAAGAACTAAAAACCCAGGTAGCACTTTCATCTGAAACTCCTAGGACAAGGTATCTAAAGGCTGTCTCTACAAGTGAATTGAAGACCGAAGGTGTTTCCCCTTATTTAATGTTGATTAGGTTACGGAAATGAACTGGCTGGATGAAGATCTGATTTAGAAAGACTGCGTGAGTCTTATTTATTCTCTGAAACACAGCCCAAGTTTCATGTTAAAATGGCAAAATGCCATTATTTAAATGGAACTTATTACATACCAATGGCTTTGCAAGAAGATGACATTTCAGAAGATCAAACAAATCTATATTTAATGGATGGACTCTTCAAAACTTACCAAATAGTTGAAGAAACCAGGTGCCTTCTCATGATGGAAGACAGATTCTGCTTTAAATTAAAAAAAAAAAAATCTGAATCTTGTTTTCAGATTTTTTTTTCTACTGGNOV15g, SNP13382487SEQ ID NO: 4321288 aaMW at 33581.0kDof CG51992-05,SNP Change:Protein Sequenceno changeMTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSTNBKLDAKLQASREAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKLEEKHSQITELENLVQRMEKEKRTLLERKLSLENKLLQLKSSATYGKSCQDLQREISILQEQISHLQFVIHSQHQNLRSVIQEMEGLKNNLKEQDKRIENLREKVNILEAQNKELKTQVALSSETPRTKVSKAVSTSELKTEGVSPYLMLIRLRK


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 15B.

TABLE 15BComparison of the NOV15 protein sequences.NOV15aMTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSNOV15b------------------------------------------------------------NOV15c------------------------------------MQSLDLLMKKIKGKDLQ-LLEMNKNOV15dMTTVTVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSNOV15eMTTVAVTTEIPPRDKMEDNSALYESTSAHIIEETEYVKKIRTTLQKIRTQMFKDEIRHDSNOV15aTNHKLDAK-----------------------------------------------LQASRNOV15b------------------------------------------------------------NOV15cENEVLKIK-----------------------------------------------LQASRNOV15dTNHKLDAKHCGNLQQGSDSEMDPSCCSLDLLMKKIKGKDLQLLEMNKENEVLKIKLQASRNOV15eTNHKLDAKHCGNLQQGSDSEMDPSCCSLDLLMKKIKGKDLQLLEMNKENEVLKIKLQASRNOV15aEAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKNOV15b------------------------------------------------------------NOV15cEAGAAALRNVAQRLFENYQTQSEEVRKKQEGSKQLLQVNKLEKEQKLKQHVENLNQVAEKNOV15dEAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKNOV15eEAGAAALRNVAQRLFENYQTQSEEVRKKQEDSKQLLQVNKLEKEQKLKQHVENLNQVAEKNOV15aLEEKHSQITELENLVQRMEKEKRTLLERYLSLENKLLQLKSSATYGKSCQDLQREISILQNOV15b---------------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(SEQ ID NO: 420)NOV15b(SEQ ID NO: 422)NOV15c(SEQ ID NO: 424)NOV15d(SEQ ID NO: 426)NOV15e(SEQ ID NO: 428)


Further analysis of the NOV15a protein yielded the following properties shown in Table 15C.

TABLE 15CProtein Sequence Properties NOV15aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 9; pos. chg 0; neg. chg 1H-region:length 3; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −11.91possible cleavage site: between 49 and 50>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERAL Likelihood = 9.07 (at 189)ALOM score: 9.07 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):1.56Hyd Moment(95):1.30G content:0D/E content:2S/T content:5Score: −5.82Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 17.4%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regions 76 A0.56 77 A0.56 78 A0.56 79 L0.56 80 R0.56 81 N0.59 82 V0.67 83 A0.67 84 Q0.67 85 R0.67 86 L0.67 87 F0.67 88 E0.67 89 N0.67 90 Y0.67 91 Q0.67 92 T0.67 93 Q0.80 94 S0.80 95 E0.80 96 E0.80 97 V0.80 98 R0.80 99 K0.81100 K0.81101 Q0.81102 E0.81103 D0.81104 S0.81105 K0.81106 Q0.81107 L0.86108 L0.98109 Q0.98110 V0.98111 N0.98112 K0.98113 L0.98114 E0.98115 K0.98116 E0.99117 Q0.99118 K0.99119 L0.99120 K0.99121 Q0.99122 H0.99123 V0.99124 E1.00125 N1.00126 L1.00127 N1.00128 Q1.00129 V1.00130 A1.00131 E1.00132 K1.00133 L1.00134 E1.00135 E1.00136 K1.00137 H1.00138 S1.00139 Q1.00140 I1.00141 T1.00142 E1.00143 L1.00144 E1.00145 N1.00146 L1.00147 V1.00148 Q1.00149 R1.00150 M1.00151 E1.00152 K1.00153 E1.00154 K1.00155 R1.00156 T1.00157 L1.00158 L1.00159 E1.00160 R1.00161 K1.00162 L1.00163 S1.00164 L1.00165 E1.00166 N1.00167 K1.00168 L1.00169 L1.00170 Q1.00171 L1.00172 K1.00173 S1.00174 S1.00175 A1.00176 T0.99177 Y0.88178 G0.83179 K0.83180 S0.83181 C0.83182 Q0.83183 D0.83184 L0.83185 Q0.83186 R0.83187 E0.83188 I0.83189 S0.83190 I0.83191 L0.83192 Q0.83193 E0.83194 Q0.83195 I0.83196 S0.83197 H0.83198 L0.83199 Q0.83200 F0.62201 V0.62202 I0.87203 H0.87204 S0.99205 Q0.99206 H1.00207 Q1.00208 N1.00209 L1.00210 R1.00211 S1.00212 V1.00213 I1.00214 Q1.00215 E1.00216 M1.00217 E1.00218 G1.00219 L1.00220 K1.00221 N1.00222 N1.00223 L1.00224 K1.00225 E1.00226 Q1.00227 D1.00228 K1.00229 R1.00230 I1.00231 E1.00232 N1.00233 L1.00234 R1.00235 E1.00236 K1.00237 V1.00238 N1.00239 I1.00240 L1.00241 E1.00242 A1.00243 Q1.00244 N1.00245 K1.00246 E1.00247 L1.00248 K1.00249 T1.00250 Q1.00251 V1.00252 A1.00253 L1.00254 S1.00255 S1.00256 E1.00257 T1.00total: 182 residuesFinal Results (k = 9/23):69.6%: nuclear13.0%: cytoplasmic 8.7%: peroxisomal 4.3%: cytoskeletal 4.3%: mitochondrial>> prediction for CG51992-05 is nuc (k = 23)


A search of the NOV15a protein against the Geneseq database, a proprietary se that contains sequences published in patents and patent publication, yielded homologous proteins shown in Table 15D.

TABLE 15DGeneseq Results for NOV15aNOV15aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABB77426Human tumour marker protein se57-1 -1 . . . 288288/335(85%)e−151Homo sapiens, 335 aa. [WO200238803-1 . . . 335288/335(85%)A2, 16-MAY-2002]AAU08663Human NOV7 protein - Homo sapiens,1 . . . 288288/335(85%)e−151335 aa. [WO200168851-A2, 20-SEP-1 . . . 335288/335(85%)2001]AAU08662Human NOV6 protein - Homo sapiens,1 . . . 288287/335(85%)e−150335 aa. [WO200168851-A2, 20-SEP-1 . . . 335287/335(85%)2001]AAU08661Human NOV5 protein - Homo sapiens,62 . . . 288 222/227(97%)e−117251 aa. [WO200168851-A2, 20-SEP-25 . . . 251 222/227(97%)2001]ABG79634Human novel secreted protein SECP10,43 . . . 258 67/221(30%)1e−21 Incyte ID No. 7939381CD1 - Homo202 . . . 415 125/221(56%)sapiens, 466 aa. [WO200262841-A2,15-AUG-2002]


In a BLAST search of public sequence databases, the NOV15a protein was found to have homology to the proteins shown in the BLASTP data in Table 15E.

TABLE 15EPublic BLASTP Results for NOV15aNOV15aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9H2F9CTCL tumor antigen se57-1 -1 . . . 288288/335(85%) e−150Homo sapiens (Human), 335 aa.1 . . . 335288/335(85%)Q8BVC4Similar to CTCL tumor antigen1 . . . 288213/333(63%) e−106SE57-1 - Mus musculus (Mouse),1 . . . 333241/333(71%)333 aa.AAH46343Hypothetical protein - Mus1 . . . 155102/200(51%)3e−43musculus (Mouse), 242 aa.1 . . . 200120/200(60%)Q96NF5Hypothetical protein FLJ30973 -43 . . . 258 67/221(30%)5e−21Homo sapiens (Human), 466 aa.202 . . . 415 125/221(56%)A40809enamelin, 44K - bovine, 389 aa.22 . . . 261 61/275(22%)6e−1539 . . . 309 131/275(47%)


PFam analysis predicts that the NOV15a protein contains the domains shown in the Table 15F.

TABLE 15FDomain Analysis of NOV15aPfamNOV15aIdentities/ExpectDomainMatch RegionSimilaritiesValuefor theMatched Region


Example 16

The NOV16 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 16A.

TABLE 16ANOV16 Sequence AnalysisNOV16a, CG52171-04SEQ ID NO: 4332403 bpDNA SequenceORF Start: ATG at 81ORF Stop: TGA at 2292GGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGCACCTCAGCAACTATGCCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16a, CG52171-04SEQ ID NO: 434737 aaMW at 81398.4kDProtein SequenceMASCPDSDNSWVLAGSESLPVETLGPASRMDPESEEALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTEIAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16b, CG52171-01SEQ ID NO: 4352739 bpDNA SequenceORF Start: ATG at 670ORF Stop: TGA at 2275CTGCAACAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTCCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCGCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATTTTCCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGGTAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGGCAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCCTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCTAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATCTTTCACACAGTAGAGCAAAATCACCAGCCCTGCACTGATGTCACTTTATGTAGAAAAAGGCCTTAGCTGGACCTGCGTTGCCGTCTATGCAAATGCATGCAAATACTCCAGGCCCTGGGATGTGGGCTTGTGTTTTGTCACTGTGAAGGGGGAGATGGGAGAGGAGCCTGTTTTGGGGTGGGGTCTGGGGAAGGCAATCTGATTCTGAAGCTAAAGAGCTTTCATCCTCTTGAGTGTATGTCCCCATAGTGGGCCCCTTGACCCACATGCTGACCGGTGCCTTGGGATTTGACTAGAGTTGCTGCTTCCAAACCTAACAAAACCCCAGGGTAAGTCCTCGTGCTGGGCCTCGNOV16b, CG52171-01SEQ ID NO: 436535 aaMW at 60290.7kDProtein SequenceMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGGRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKGSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16c, CG52171-02SEQ ID NO: 4371665 bpDNA SequenceORF Start: ATG at 77ORF Stop: TGA at 1388CAACAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGGTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCTAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATCTTTCACACAGTAGAGCAAAATCACCAGCCCTGCACTGATGTCACTTTATGTAGAAAAAGGCCTTAGCTGGACCTGCGTTGCCGTCTATGCAAATGCATGCAAATACTCCAGGCCCTGGGATGTGGGCTTGTGTTTTGTCACTGTGAAGGGGGAGATGGGAGAGGANOV16c, CG52171-02SEQ ID NO: 438437 aaMW at 47136.5kDProtein SequenceMASCPDSDNSWVLAGSESLPVETLGPASRMDPESEHALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLPRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALGLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLPARLQGLEADCVRGPDGDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRSGKKDKHSQSPPAAGPREGHSHSHHHHHRGNOV16d, CG52171-03SEQ ID NO: 4392104 bpDNA SequenceORF Start: ATG at 76ORF Stop: at 2104AACAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGGTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGGTAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGGCAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCCTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTTCTAGAATTCAGCGGCCGCTNOV16d, CG52171-03SEQ ID NO: 440676 aaMW at 74306.8kDProtein SequenceMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALGLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGGRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKGSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVLEFSGRNOV16e, CG52171-05SEQ ID NO: 4411539 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCCTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCNOV16e, CG52171-05SEQ ID NO: 442513 aaMW at 58278.1kDProtein SequenceESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYPAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRSGKKDKHSQSPPAAGPREGHSHSHHHHHRGNOV16f, CG52171-06SEQ ID NO: 4432366 bpDNA SequenceORF Start: ATG at 76ORF Stop: TGA at 2266GAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAATCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGCCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCCTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCTAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGNOV16f, CG52171-06SEQ ID NO: 444730 aaMW at 80563.4kDProtein SequenceMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLNQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQAPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRSGKKDKHSQSPRAAGPREGHSHSHHHHRGNOV16g, 5NP13374628SEQ ID NO: 4452403 bpof CG52171-04,ORF Start: ATG at 81ORF Stop: TGA at 2292DNA SequenceSNP Pos: 373SNP Change: A to GGGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGGCACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16g, SNP13374628SEQ ID NO: 446737 aaMW at 81340.4kDof CG52171-04,SNP Pos: 98SNP Change:Protein SequenceAsp to GlyMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGGTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALEsELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRPLLGSVQQDLERSLQDASRGDPAHAGLAELGHPLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16h, SNP13377329SEQ ID NO: 4472403 bpof CG52171-04,ORF Start: ATG at 81ORF Stop: TGA at 2292DNA SequenceSNP Pos: 1150SNP Change: A to GGGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGOCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGGTAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16h, SNP13377329SEQ ID NO: 448737 aaMW at 81340.4kDof CG52171-04,SNP Pos: 357SNP Change:Protein SequenceAsp to GlyMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGGRGPQGDKAIRBQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16i, SNP13374627SEQ ID NO: 4492403 bpof CG52171-04,ORF Start: ATG at 81ORF Stop: TGA at 2292DNA SequenceSNP Pos: 1903SNP Change: C to TGGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTQCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGTCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16i, SNP13374627SEQ ID NO: 450737 aaMW at 81426.5kDof CG52171-04,SNP Pos: 608SNP Change:Protein SequenceAla to ValMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELVPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16j, SNP13374626SEQ ID NO: 4512403 bpof CG52171-04,ORF Start: ATG at 81ORF Stop: TGA at 2292DNA SequenceSNP Pos: 1918SNP Change:A to GGGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACGGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16j, SNP13374626SEQ ID NO: 452737 aaMW at 81426.5kDof CG52171-04,SNP Pos: 613SNP Change:Protein SequenceGln to ArgMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYHAPQGCSGVDECARQEGLTFFGTELAPVRQRELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16k, SNP13374625SEQ ID NO: 4532403 bpof CG52171-04,ORF Start: ATG at 81ORF Stop: TGA at 2292DNA SequenceSNP Pos: 1951SNP Change: C to TGGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGGACCTCAGCAACTATGGCCTCCTGCCCAGACTCTQATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGTACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16k, SNP13374625SEQ ID NO: 454737 aaMW at 81426.5kDof CG52171-04,SNP Pos: 624SNP Change:Protein SequenceAla to ValMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLVRLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRGNOV16l, SNP13374624SEQ ID NO: 4552403 bpof CG52171-04,ORF Start: ATG at 81ORF Stop: TGA at 2292DNA SequenceSNP Pos: 2124SNP Change: G to TGGCACGAGAAGTTAAGCTGAAGACCGAAGCAAGAGCTGGTTCAGGTGGCAGCCACAGCAGCCTCAGGCACCTCAGCAACTATGGCCTCCTGCCCAGACTCTGATAATAGCTGGGTGCTTGCTGGCTCCGAGAGCCTGCCAGTGGAGACACTGGGCCCGGCATCCAGGATGGACCCAGAATCTGAGAGAGCCCTGCAGGCCCCTCACAGCCCCTCCAAGACAGATGGGAAAGAATTAGCTGGGACCATGGATGGAGAAGGGACGCTCTTCCAGACTGAAAGCCCTCAGTCTGGCAGCATTCTAACAGAGGAGACTGAGGTCAAGGGCACCCTGGAAGGTGATGTTTGTGGTGTGGAGCCTCCTGGCCCAGGAGACACAGTAGTCCAGGGAGACCTGCAGGAGACCACCGTGGTGACAGGCCTGGGACCAGACACACAGGACCTGGAAGGCCAGAGCCCTCCACAGAGCCTGCCTTCAACCCCCAAAGCAGCTTGGATCAGGGAGGAGGGCCGCTGCTCCAGCAGTGACGATGACACCGACGTGGACATGGAGGGTCTGCGGAGACGGCGGGGCCGGGAGGCCGGCCCACCTCAGCCCATGGTGCCCCTGGCTGTGGAGAACCAGGCTGGGGGTGAGGGTGCAGGCGGGGAGCTGGGCATCTCCCTCAACATGTGCCTCCTTGGGGCCCTGGTTCTGCTTGGCCTGGGGGTCCTCCTCTTCTCAGGTGGCCTCTCAGAGTCTGAGACTGGGCCCATGGAGGAAGTGGAGCGGCAGGTCCTCCCAGACCCCGAGGTGCTGGAAGCTGTGGGGGACAGGCAGGATGGGCTAAGGGAACAGCTGCAGGCCCCAGTGCCTCCTGACAGTGTCCCCAGCCTGCAAAACATGGGTCTTCTGCTGGACAAGCTGGCCAAGGAGAACCAGGACATCCGGCTGCTGCAGGCCCAGCTGCAGGCCCAAAAGGAAGAGCTTCAGAGCCTGATGCACCAGCCCAAAGGGCTAGAGGAGGAGAATGCCCAGCTCCGGGGGGCTCTGCAGCAGGGCGAAGCCTTCCAGCGGGCTCTGGAGTCAGAGCTGCAGCAGCTGCGGGCCCGGCTCCAGGGGCTGGAGGCCGACTGTGTCCGGGGCCCAGATGGGGTGTGCCTCAGTGGGGATAGAGGCCCACAGGGTGACAAGGCCATCAGGGAGCAAGGCCCCAGGGAGCAGGAGCCAGAACTCAGCTTCCTGAAGCAGAAGGAACAGCTGGAGGCTGAGGCACAGGCATTAAGGCAAGAGTTAGAGAGGCAGCGACGGCTGCTGGGGTCTGTACAGCAGGATCTGGAGAGGAGCTTGCAGGATGCCAGCCGCGGGGACCCAGCTCATGCTGGCTTGGCTGAGCTGGGCCACAGATTGGCCCAGAAACTGCAGGGCCTGGAGAACTGGGGCCAGGACCCTGGGGTCTCTGCCAATGCCTCAAAGGCCTGGCACCAGAAGTCCCACTTCCAGAATTCTAGGGAGTGGAGTGGAAAGGAAAAGTGGTGGGATGGGCAGAGAGACCGGAAGGCTGAGCACTGGAAACATAAGAAGGAAGAATCTGGCCGGGAAAGGAAGAAGAACTGGGGAGGTCAGGAGGACAGGGAGCCAGCAGGAAGGTGGAAGGAGGGCAGGCCAAGGGTGGAGGAGTCGGGGAGCAAGAAGGAGGGCAAGCGACAGGGCCCGAAGGAACCCCCAAGGAAAAGTGGTAGCTTCCACTCCTCTGGAGAAAAGCAGAAGCAACCTCGGTGGAGGGAAGGGACTAAGGACAGCCATGACCCCCTGCCATCCTGGGCAGAGCTGTTGAGGCCCAAGTACCGGGCACCCCAGGGCTGCTCAGGTGTGGACGAGTGTGCCCGGCAGGAGGGCTTGACTTTCTTTGGCACAGAGCTAGCCCCAGTGCGGCAACAGGAGCTGGCCTCTCTGCTAAGAACATACTTGGCACGGCTGCCCTGGGCTGGGCAGCTGACCAAGGAGCTACCCCTCTCACCTGCTTTCTTTGGTGAGGATGGCATCTTCCGTCATGACCGCCTCCGCTTCCGGGATTTTGTGGATGCCCTGGAGGACAGCTTGGAGGAGGTGGCTGTGCAACAGACAGGTGATGATGATGAAGTATATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTGGAGACAAAGCACTGAAGAAGAGGCCTCTTCCTCCCCACAGGTCAGGGAAGAAGGACAAGCACTCACAGAGCCCAAGAGCTGCGGGGCCCAGGGAGGGGCACAGCCATAGCCACCACCACCACCACCGGGGCTGACACCCTGCCCCACAGGGAATGGCCTTGGCCTGGCCCAGCCCAAGATCCCAGCGTTATCCAACTCCTGGAGGGTGGACTCTGTCCTGGCTTGTTTGGTGTCCTCAGATATNOV16l, SNP13374624SEQ ID NO: 456737 aaMW at 81446.5kDof CG52171-04,SNP Pos: 682SNP Change:Protein SequenceAsp to TyrMASCPDSDNSWVLAGSESLPVETLGPASRMDPESEHALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLMHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVKWWDGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKEPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVDALEDSLEEVAVQQTGDDDEVYDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPRAAGPREGHSHSHHHHHRG


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 16B.

TABLE 16BComparison of the NOV16 protein sequences.NOV16aMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTNDGEGNOV16b------------------------------------------------------------NOV16cMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGNOV16dMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGNOV16e------------------------------------------------------------NOV16fMASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGNOV16aTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDNOV16b------------------------------------------------------------NOV16cTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDNOV16dTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDNOV16e------------------------------------------------------------NOV16fTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDNOV16aLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENNOV16b------------------------------------------------------------NOV16cLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENNOV16dLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRPRGREAGPPQPMVPLAVENNOV16e------------------------------------------------------------NOV16fLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENNOV16aQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLENOV16b----------------MCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLENOV16cQAGGEGAGGELGISLNMCLLGALGLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLENOV16dQAGGEGAGGELGISLNMCLLGALGLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLENOV16e--------------------------------------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----------NOV16dHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGGRGPNOV16eHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARLQGLEADCVRGPDGVCLSGDRGPNOV16fHQPKGLEEENAQLRGALQQGEAFQRALESELQQLRARIQGLEADCVRGPDGVCLSGDRGPNOV16aQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASNOV16bQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASNOV16c------------------------------------------------------------NOV16dQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASNOV16eQGDKAIREQGPREQEPELSFLKQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASNOV16fQGDKAIREQGPREQEPELSFLNQKEQLEAEAQALRQELERQRRLLGSVQQDLERSLQDASNOV16aRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDNOV16bRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDNOV16c------------------------------------------------------------NOV16dRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDNOV16eRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDNOV16fRGDPAHAGLAELGHRLAQKLQGLENWGQDPGVSANASKAWHQKSHFQNSREWSGKEKWWDNOV16aGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKENOV16bGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKENOV16c------------------------------------------------------------NOV16dGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKENOV16eGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQGPKENOV16fGQRDRKAEHWKHKKEESGRERKKNWGGQEDREPAGRWKEGRPRVEESGSKKEGKRQAPKENOV16aPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLNOV16bPPRKSGSFHSSGEKQKQPRWREGTKGSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLNOV16c------------------------------------------------------------NOV16dPPRKSGSFHSSGEKQKQPRWREGTKGSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLNOV16ePPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLNOV16fPPRKSGSFHSSGEKQKQPRWREGTKDSHDPLPSWAELLRPKYRAPQGCSGVDECARQEGLNOV16aTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVNOV16bTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVNOV16c--------------------------------------------GDGIFRHDRLRFRDFVNOV16dTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVNOV16eTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVNOV16fTFFGTELAPVRQQELASLLRTYLARLPWAGQLTKELPLSPAFFGEDGIFRHDRLRFRDFVNOV16aDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKRPLPPHRSGKKDKHSQSPPANOV16bDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKR------SGKKDKHSQSPRANOV16cDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKR------SGKKDKHSQSPRANOV16dDALEDSLEEVLEFSGR--------------------------------------------NOV16eDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKR------SGKKDKHSQSPRANOV16fDALEDSLEEVAVQQTGDDDEVDDFEDFIFSHFFGDKALKKR------SGKKDKHSQSPPANOV16aAGPREGHSHSHHHHHRGNOV16bAGPREGHSHSHHHHHRGNOV16cAGPREGHSHSHHHHHRGNOV16d-----------------NOV16eAGPREGHSHSHHHHHRGNOV16fAGPREGHSHSHHHHRG-NOV16a(SEQ ID NO: 434)NOV16b(SEQ ID NO: 436)NOV16c(SEQ ID NO: 438)NOV16d(SEQ ID NO: 440)NOV16e(SEQ ID NO: 442)NOV16f(SEQ ID NO: 444)


Further analysis of the NOV16a protein yielded the following properties shown in Table 16C.

TABLE 16CProtein Sequence Properties NOV16aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 8; pos. chg 0; neg. chg 2H-region:length 8; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −7.85possible cleavage site: between 23 and 24>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 197-213−10.46PERIPHERALLikelihood = 9.34 (at 12)ALOM score: −10.46 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 204Charge difference: −1.0 C(−4.0)-N(−3.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 197)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):2.09Hyd Moment(95):1.02G content:0D/E content:2S/T content:2Score: −7.60Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: RRRR (5) at 160pat4: KHKK (3) at 491pat4: KKRP (4) at 699pat7: nonebipartite: nonecontent of basic residues: 12.9%NLS Score: 0.28KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regions263 S0.81264 L0.87265 Q0.96266 N0.97267 M0.98268 G0.99269 L1.00270 L1.00271 L1.00272 D1.00273 K1.00274 L1.00275 A1.00276 K1.00277 E1.00278 N1.00279 Q1.00280 D1.00281 I1.00282 R1.00283 L1.00284 L1.00285 Q1.00286 A1.00287 Q1.00288 L1.00289 Q1.00290 A1.00291 Q1.00292 K1.00293 E1.00294 E1.00295 L1.00296 Q1.00297 S1.00298 L1.00299 M1.00300 H1.00301 Q1.00303 K0.71304 G0.71305 L0.87306 E0.87307 E0.87308 E0.87309 N0.87310 A0.87311 Q0.87312 L0.87313 R0.87314 G0.87315 A0.90316 L0.94317 Q0.94318 Q0.94319 G0.94320 E0.94321 A0.94322 F0.94323 Q0.94324 R0.94325 A0.94326 L0.94327 E0.94328 S0.94329 E0.94330 L0.94331 Q0.94332 Q0.94333 L0.94334 R0.94335 A0.94336 R0.94337 L0.94338 Q0.94339 G0.94340 L0.94341 E0.94342 A0.94343 D0.94344 C0.79376 E0.95377 L1.00378 S1.00379 F1.00380 L1.00381 K1.00382 Q1.00383 K1.00384 E1.00385 Q1.00386 L1.00387 E1.00388 A1.00389 E1.00390 A1.00391 Q1.00392 A1.00393 L1.00394 R1.00395 Q1.00396 E1.00397 L1.00398 E1.00399 R1.00400 Q1.00401 R1.00402 R1.00403 L1.00404 L1.00405 G1.00406 S1.00407 V1.00408 Q1.00409 Q1.00410 D1.00411 L1.00412 E1.00413 R1.00414 S0.99415 L0.94416 Q0.94417 D0.92418 A0.92419 S0.78420 R0.78421 G0.54total: 127 residuesFinal Results (k = 9/23):39.1%: nuclear26.1%: mitochondrial 8.7%: cytoplasmic 8.7%: Golgi 4.3%: plasma membrane 4.3%: vesicles of secretory system 4.3%: extracellular, including cell wall 4.3%: peroxisomal>> prediction for CG52171-04 is nuc (k = 23)


A search of the NOV16a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 16D.

TABLE 16DGeneseq Results for NOV16aNOV16aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAB93999Human protein sequence SEQ ID30 . . . 737 700/708 (98%)0.0NO: 14107 - Homo sapiens, 702 aa.1 . . . 702700/708 (98%)[EP1074617-A2, 07-FEB-2001]ABG20800Novel human diagnostic protein7 . . . 700624/697 (89%)0.0#20791 - Homo sapiens, 669 aa.2 . . . 668634/697 (90%)[WO200175067-A2, 11-OCT-2001]AAB95336Human protein sequence SEQ ID1 . . . 566564/566 (99%)0.0NO: 17611 - Homo sapiens, 662 aa.1 . . . 566566/566 (99%)[EP1074617-A2, 07-FEB-2001]AAB62402Human MBSP6 polypeptide (clone197 . . . 737 533/541 (98%)0.03207791.0.128) - Homo sapiens, 5351 . . . 535533/541 (98%)aa. [WO200127277-A2, 19-APR-2001]AAB80449Gene #23 associated peptide #1 -17 . . . 397 375/381 (98%)0.0Homo sapiens, 451 aa.8 . . . 388377/381 (98%)[WO200107459-A1, 01-FEB-2001]


In a BLAST search of public sequence databases, the NOV16a protein was found to have homology to the proteins shown in the BLASTP data in Table 16E.

TABLE 16EPublic BLASTP Results for NOV16aNOV16aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9HD85Hematopoietic PBX-interacting1 . . . 737730/737 (99%)0.0protein - Homo sapiens (Human),1 . . . 731730/737 (99%)731 aa.Q96AQ6Hematopoietic PBX-interacting1 . . . 737730/737 (99%)0.0protein - Homo sapiens (Human),1 . . . 731730/737 (99%)731 aa.Q9HA02Hypothetical protein FLJ12435 -30 . . . 737 700/708 (98%)0.0Homo sapiens (Human), 702 aa.1 . . . 702700/708 (98%)Q9H8X6Hypothetical protein FLJ13157 -1 . . . 566564/566 (99%)0.0Homo sapiens (Human), 662 aa.1 . . . 566566/566 (99%)CAC43890Sequence 11 from Patent197 . . . 737 533/541 (98%)0.0WO0127277 - Homo sapiens1 . . . 535533/541 (98%)(Human), 535 aa.


PFam analysis predicts that the NOV16a protein contains the domains shown in the Table 16F.

TABLE 16FDomain Analysis of NOV16aPfamNOV16aIdentities/ExpectDomainMatch RegionSimilaritiesValuefor theMatched Region


Example 17.

The NOV17 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 17A.

TABLE 17ANOV17 Sequence AnalysisNOV17a, CG52534-06SEQ ID NO: 4571275 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1273ATGGAGCGGCTTTGGGGTCTATTCCAGAGAGCGCAACAACTGTCCCCAAGATCCTCTCAGACCGTCTACCAGCGTGTGGAAGGCCCCCGGAAAGGGCACCTGGAGGAGGAAGAGGAAGACGGGGAGGAGGGGGCGGAGACATTGGCCCACTTCTGCCCCATGGAGCTGAGGGGCCCTGAGCCCCTGGGCTCTAGACCCAGGCAGCCAAACCTCATTCCCTGGGCGGCAGCAGGACGGAGGGCTGCCCCCTACCTGGTCCTGACGGCCCTGCTGATCTTCACTGGGGCCTTCCTACTGGGCTACGTCGCCTTCCGAGGGTCCTGCCAGGCGTGCGGAGACTCTGTGTTGGTGGTCAGTGAGGATGTCAACTATGAGCCTGACCTGGATTTCCACCAGGGCAGACTCTACTGGAGCGACCTCCAGGCCATGTTCCTGCAGTTCCTGGGGGAGGGGCGCCTGGAGGACACCATCAGGCAAACCAGCCTTCGGGAACGGGTGGCAGGCTCGGCCGGGATGGCCGCTCTGACTCAGGACATTCGCGCGGCGCTCTCCCGCCAGAAGCTGGACCACGTGTGGACCGACACGCACTACGTGGGGCTGCAATTCCCGGATCCGGCTCACCCCAACACCCTGCACTGGGTCGATGAGGCCGGGAAGGTCGGAGAGCAGCTGCCGCTGGAGGACCCTGACGTCTACTGCCCCTACAGCGCCATCGGCAACGTCACGGGAGAGCTGGTGTACGCCCACTACGGGCGGCCCGAAGACCTGCAGGACCTGCGGGCCAGGGGCGTGGATCCAGTGGGCCGCCTGCTGCTGGTGCGCGTGGGGGTGATCAGCTTCGCCCAGAAGGTGACCAATGCTCAGGACTTCGGGGCTCAAGGAGTGCTCATATACCCAGAGCCAGCGGACTTCTCCCAGGACCCACCCAAGCCAAGCCTGTCCAGCCAGCAGGCAGTGTATGGACATGTGCACCTGGGAACTGGAGACCCCTACACACCTGGCTTCCCTTCCTTCAATCAAACCCAGTTCCCTCCAGTTGCATCATCAGGCCTTCCCAGCATCCCAGCCCAGCCCATCAGTGCAGACATTGCCTCCCGCCTGCTGGACCACCTGCGGCTGCTGCGCTCCAACAGCTCCGGGACCCCCGGGGCCACCTCCTCCACTGGCTTCCAGGAGAGCCGTTTCCGGCGTCAGCTAGCCCTGCTCACCTGGACGCTGCAAGGGGCAGCCAATGCGCTTAGCGGGGATGTCTGGAACATTGATAACAACTTCTGANOV17a, CG52534-06SEQ ID NO: 458424aaMW at 46603.7kDProtein SequenceMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPEPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRGSCQACGDSVLVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTQDIPAALSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPAQPISADIASRLLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLALLTWTLQGAANALSGDVWNIDNNFNOV17b, CG52534-01SEQ ID NO: 4592857 bpDNA SequenceORF Start: ATG at 28ORF Stop: TGA at 1495CTGCAGGCTTCAGGAGGGGACACAAGCATGGAGCGGCTTTGGGGTCTATTCCAGAGAGCGCAACAACTGTCCCCAAGATCCTCTCAGACCGTCTACCAGCGTGTGGAAGGCCCCCGGAAAGGGCACCTGGAGGAGGAAGAGGAAGACGGGGAGGAGGGGGCGGAGACATTGGCCCACTTCTGCCCCATGGAGCTGAGGGGCCCTGAGCCCCTGGGCTCTAGACCCAGGCAGCCAAACCTCATTCCCTGGGCGGCAGCAGGACGGAGGGCTGCCCCCTACCTGGTCCTGACGGCCCTGCTGATCTTCACTGGGGCCTTCCTACTGGGCTACGTCGCCTTCCGAGGGTCCTGCCAGGCGTGCGGAGACTCTGTGTTGGTGGTCAGTGAGGATGTCAACTATGAGCCTGACCTGGATTTCCACCAGGGCAGACTCTACTGGAGCGACCTCCAGGCCATGTTCCTGCAGTTCCTGGGGGAGGGGCGCCTGGAGGACACCATCAGGCAAACCAGCCTTCGGGAACGGGTGGCAGGCTCGGCCGGGATGGCCGCTGCTGACTCAGGACATTCGCGCGGCCTCTCCCGCCAGAAGCTGGACCACGTGTGGACCGACACGCACTACGTGGGGCTGCAATTCCCGGATCCGGCTCACCCCAACACCCTGCACTGGGTCGATGAGGCCGGGAAGGTCGGAGAGCAGCTGCCGCTGGAGGACCCTGACGTCTACTGCCCCTACAGCGCCATCGGCAACGTCACGGGAGAGCTGGTGTACGCCCACTACGGGCGGCCCGAAGACCTGCAGGACCTGCGGGCCAGGGGCGTGGATCCAGTGGGCCGCCTGCTGCTGGTGCGCGTGGGGGTGATCAGCTTCGCCCAGAAGGTGACCAATGCTCAGGACTTCGGGGCTCAAGGAGTGCTCATATACCCAGAGCCAGCGGACTTCTCCCAGGACCCACCCAAGCCAAGCCTGTCCAGCCAGCAGGCAGTGTATGGACATGTGCACCTGGGAACTGGAGACCCCTACACACCTGGCTTCCCTTCCTTCAATCAAACCCAGTTCCCTCCAGTTGCATCATCAGGCCTTCCCAGCATCCCACGCCGAGCCCTATGGAGTCTCAAAAAAAAAAAGGGCCCCGGGCCACGACTGCGGCTAGTGGTCAACAATCACAGGACCTCCACCCCCATCAACAACATCTTCGGCTGCATCGAAGGCCGCTCAGAGCCAGATCACTACGTTGTCATCGGGGCCCAGAGGGATGCATGGGGCCCAGGAGCAGCTAAATCCGCTGTGGGGACGGCTATACTCCTGGAGCTGGTGCGGACCTTTTCCTCCATGGTGAGCAACGGCTTCCGGCCCCGCAGAAGTCTCCTCTTCATCAGCTGGGACGGTGGTGACTTTGGAAGCGTGGGCTCCACGGAGTGGCNTAGAGGGCTACCTCAGCGTGCTGCACCTCAAAGCCGTAGTGTACGTGAGCCTGGACAACGCAGTGAAGGGGGATGACAAGTTTCATGCCAAGACCAACCCCCTTCTGACAAGTCTCATTGAGAAGTGTCCTGAAGCAGATGGATTCTCCCAACCACAGTGGGCAGACTCTCTATGAACAGGTGGGGTTCACCAATTCCCAGCTGGGATTGCTGAGGTGATCCGGCCCCTACCCATGGGACAGCAGTGCCTATTTCCTTCACGGGCCTTTGTTGGGGAGTCCCTTGCCCTTCGAGTTCTTCCTTTTATTGGAGGGACGGACCAGGCCTTACCCATTTCCTTGCACAAAAAGGGAGGGACACTTATTGGAGGAACCTGCAGTAAGGTGCTGCAAGGCCGCCTGCCCGCCGTGGCCCAGGCCGTGGCCCAGCTCGCAGGGCAGCTCCTCATCCGGCTCAGCCACGATCGCCTGCTGCCCCTCGACTTCGGCCGCTACGGGGACGTCGTCCTCAGGCACATCGGGAACCTCAACGAGTTCTCTGGGGACCTCAAGCCCGCGGGCTGACCCTGCAGTGGGTGTACTCGGCGCGGGGGGACTACATCCGGGCGGCGGAAAAGCTGCGGCAGGTAGATCTACTTGCCGCAGCTCGGAGGAGAGAGACGAGCGACTGACACGCATGTACAACGTCCGCATAATGCGGGTGGAGTTCTACTTCCTTTCCCAGTACGTGTCGCCAGCCGACTCCCCGTTCCGCCACATCTTCATGGGCCGTGGAGACCACACGCTGGGCGCCCTGCTGGACCACCTGCGGCTGCTGCACTCCAACAGCTCCGGGACCCCCGGGGCCACCTCCTCCACTGGCTTCCAGGGAGAGCCGTTTCCGGGTCGTCAGCTAGCCCTGCTCACCTGGACGCTGCAAGGGGCAGCCAATGCGCTTAGCGGGGATGTCTGGAACATTGATAACAACTTCTGAGGCCCTGGGGATCCTCAAATCCCCGTCCCCCAGTCAAGAGCTCCTCTGCTCCTCGCTTGAATGATTCAGGGTCAGGGAGGTGGCTCAGAGTCCACCTCTCATTGCTGATCAATTTCTCATTACCCCTACACATCTCTCCACGGAGCCCAGACCCCAGCACAGATATCCACACACCCCAGCCCTGCAGTGTAGCTGACCCTAATGTGACGGTCATACTGTCGGTTAATCAGAGAGTAGCATCCCTTCAATCACAGCCCCTTCCCCTTTCTGGGGTCCTCCATACCTAGAGACCACTCTGGGAGGTTTGCTAGGCCCTGGGACCTGGCCAGCTCTGTTAGTGGGAGAGATCGCTGGCACCATAGCCTTATGGCCAACAGGTGGTCTGTGGTGAAAGGGGCGTGGAGTTTCAATATCAATAAACCACCTGATATCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAANOV17b, CG52534-01SEQ ID NO: 460489 aaMW at 53555.7kDProtein SequenceMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPEPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRGSCQACGDSVLVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAAADSGHSRGLSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPRRALWSLKKKKGPGPRLRLVVNNHRTSTPINNIFGCIEGRSEPDHYVVIGAQRDAWGPGAAKSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWDGGDFGSVGSTEWXRGLPQRAAPQSRSVREPGQRSEGGNOV17c, CG52534-02SEQ ID NO: 461865 bpDNA SequenceORF Start: ATG at 27ORF Stop: TGA at 837TGCGCGCGGATTCTGCTTCTCAGAAGATGCACTATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCCTTTTCAGCTACAACATCATCTTCTGGTTGGCTGGAGTTGTCTTCCTTGGAGTCGGGCTGTGGGCATGGAGCGAAAAGGGTGTGCTGTCCGACCTCACCAAAGTGACCCGGATGCATGGAATCGACCCTGTGGTGCTGGTCCTGATGGTGGGCGTGGTGATGTTCACCCTGGGGTTCGCCGGCTGCGTGGGGGCTCTGCGGGAGAATATCTGCTTGCTCAACTTTTTCTGTGGCACCATCGTGCTCATCTTCTTCCTGGAGCTGGCTGTGGCCGTGCTGGCCTTCCTGTTCCAGGACTGGGTGAGGGACCGGTTCCGGGAGTTCTTCGAGAGCAACATCAAGTCCTACCGGGACGATATCGATCTGCAAAACCTCATCGACTCCCTTCAGAAAGCTAACCAGTGCTGTGGCGCATATGGCCCTGAAGACTGGGACCTCAACGTCTACTTCAATTGCAGCGGTGCCAGCTACAGCCGAGAGAAGTGCGGGGTCCCCTTCTCCTGCTGCGTGCCAGATCCTGTGCAAAAAGTTGTGAACACACAGTGTGGATATGATGTCAGGATTCAGCTGAAGAGCAAGTGGGATGAGTCCATCTTCACGAAAGGCTGCATCCAGGCGCTGGAAAGCTGGCTCCCGCGGAACATTTACATTGTGGCTGGCGTCTTCATCGCCATCTCGCTGTTGCAGATATTTGGCATCTTCCTGGCAAGGACGCTGATCTCAGACATCGAGGCAGTGAAGGCCGGCCATCACTTCTGAGGAGCAGAGTTGAGGGAGCCGAGCTANOV17c, CG52534-02SEQ ID NO: 462270 aaMW at 30718.5kDProtein SequenceMHYYRYSNAKVSCWYKYLLFSYNIIFWLAGVVFLGVGLWAWSEKGVLSDLTKVTRMHGIDPVVLVLMVGVVMFTLGFAGCVGALRENICLLNFFCGTIVLIFFLELAVAVLAFLFQDWVRDRFREFFESNIKSYRDDIDLQNLIDSLQKANQCCGAYGPEDWDLNVYFNCSGASYSREKCGVPFSCCVPDPVQKVVNTQCGYDVRIQLKSKWDESIFTKGCIQALESWLPRNIYIVAGVFIAISLLQIFGIFLARTLISDIEAVKAGHHFNOV17d, CG52534-03SEQ ID NO: 463863 bpDNA SequenceORF Start: ATG at 26ORF Stop: TGA at 836GCGCGCGGATTCTGCTTCTCAGAAGATGCACTATTATAGATACTCTAACGCCAAGGTCAGCTGCTGGTACAAGTACCTCCTTTTCAGCTACAACATCATCTTCTGGTTGGCTGGAGTTGTCTTCCTTGGAGTCGGGCTGTGGGCATGGAGCGAAAAGGGTGTGCTGTCCGACCTCACCAAAGTGACCCGGATGCATGGAATCGACCCTGTGGTGCTGGTCCTGATGGTGGGCGTGGTGATGTTCACCCTGGGGTTCGCCGGCTGCGTGGGGGCTCTGCGGGAGAATATCTGCTTGCTCAACTTTTTCTGTGGCACCATCGTGCTCATCTTCTTCCTGGAGCTGGCTGTGGCCGTGCTGGCCTTCCTGTTCCAGGACTGGGTGAGGGACCGGTTCCGGGAGTTCTTCGAGAGCAACATCAAGTCCTACCGGGACGATATCGATCTGCAAAACCTCATCGACTCCCTTCAGAAAGCTAACCAGTGCTGTGGCGCATATGGCCCTGAAGACTGGGACCTCAACGTCTACTTCAATTGCAGCGGTGCCAGCTACAGCCGAGAGAAGTGCGGGGTCCCCTTCTCCTGCTGCGTGCCAGATCCTGCGCAAAAAGTTGTGAACACACAGTGTGGATATGATGTCAGGATTCAGCTGAAGAGCAAGTGGGATGAGTCCATCTTCACGAAAGGCTGCATCCAGGCGCTGGAAAGCTGGCTCCCGCGGAACATTTACATTGTGGCTGGCGTCTTCATCGCCATCTCGCTGTTGCAGATATTTGGCATCTTCCTGGCAAGGACGCTGATCTCAGACATCGAGGCAGTGAAGGCCGGCCATCACTTCTGAGGAGCAGAGTTGAGGGAGCCGAGCTNOV17d, CG52534-03SEQ ID NO: 464270 aaMW at 30690.5kDProtein SequenceMHYYRYSNAKVSCWYKYLLFSYNIIFWLAGVVFLGVGLWAWSEKGVLSDLTKVTRMHGIDPVVLVLMVGVVMFTLGFAGCVGALRENICLLNFFCGTIVLIFFLELAVAVLAFLFQDWVRDRFREFFESNIKSYRDDIDLQNLIDSLQKANQCCGAYGPEDWDLNVYFNCSGASYSREKCGVPFSCCVPDPAQKVVNTQCGYDVRIQLKSKWDESIFTKGCIQALESWLPRNIYIVAGVFIAISLLQIFGIFLARTLISDIEAVKAGHHFNOV17e, CG52534-04SEQ ID NO: 4652406 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 2404ATGGAGCGGCTTTGGGGTCTATTCCAGAGAGCGCAACAACTGTCCCCAAGATCCTCTCAGACCGTCTACCAGCGTGTGGAAGGCCCCCGGAAAGGGCACCTGGAGGAGGAAGAGGAAGACGGGGAGGAGGGGGCGGAGACATTGGCCCACTTCTGCCCCATGGAGCTGAGGGGCCCTGAGCCCCTGGGCTCTAGACCCAGGCAGCCAAACCTCATTCCCTGGGCGGCAGCAGGACGGAGGGCTGCCCCCTACCTGGTCCTGACGGCCCTGCTGATCTTCACTGGGGCCTTCCTACTGGGCTACGTCGCCTTCCGAGGGTCCTGCCAGGCGTGCGGAGACTCTGTGTTGGTGGTCAGTGAGGATGTCAACTATGAGCCTGACCTGGATTTCCACCAGGGCAGACTCTACTGGAGCGACCTCCAGGCCATGTTCCTGCAGTTCCTGGGGGAGGGGCGCCTGGAGGACACCATCAGGCAAACCAGCCTTCGGGAACGGGTGGCAGGCTCGGCCGGGATGGCCGCTCTGACTCAGGACATTCGCGCGGCGCTCTCCCGCCAGAAGCTGGACCACGTGTGGACCGACACGCACTACGTGGGGCTGCAATTCCCGGATCCGGCTCACCCCAACACCCTGCACTGGGTCGATGAGGCCGGGAAGGTCGGAGAGCAGCTGCCGCTGGAGGACCCTGACGTCTACTGCCCCTACAGCGCCATCGGCAACGTCACGGGAGAGCTGGTGTACGCCCACTACGGGCGGCCCGAAGACCTGCAGGACCTGCGGGCCAGGGGCGTGGATCCAGTGGGCCGCCTGCTGCTGGTGCGCGTGGGGGTGATCAGCTTCGCCCAGAAGGTGACCAATGCTCAGGACTTCGGGGCTCAAGGAGTGCTCATATACCCAGAGCCAGCGGACTTCTCCCAGGACCCACCCAAGCCAAGCCTGTCCAGCCAGCAGGCAGTGTATGGACATGTGCACCTGGGAACTGGAGACCCCTACACACCTGGCTTCCCTTCCTTCAATCAAACCCAGTTCCCTCCAGTTGCATCATCAGGCCTTCCCAGCATCCCAGCCCAGCCCATCAGTGCAGACATTGCCTCCCGCCTGCTGAGGAAGCTCAAAGGCCCTGTGGCCCCCCAAGAATGGCAGGGGAGCCTCCTAGGCTCCCCTTATCACCTGGGCCCCGGGCCACGACTGCGGCTAGTGGTCAACAATCACAGGACCTCCACCCCCATCAACAACATCTTCGGCTGCATCGAAGGCCGCTCAGAGCCAGATCACTACGTTGTCATCGGGGCCCAGAGGGATGCATGGGGCCCAGGAGCAGCTAAATCCGCTGTGGGGACGGCTATACTCCTGGAGCTGGTGCGGACCTTTTCCTCCATGGTGAGCAACGGCTTCCGGCCCCGCAGAAGTCTCCTCTTCATCAGCTGGGACGGTGGTGACTTTGGAAGCGTGGGCTCCACGGAGTGGCTAGAAGGCTACCTCAGCGTGCTGCACCTCAAAGCCGTAGTGTACGTGAGCCTGGACAACGCAGTGCTGGGGGATGACAAGTTTCATGCCAAGACCAGCCCCCTTCTGACAAGTCTCATTGAGAGTGTCCTGAAGCAGGTGGATTCTCCCAACCACAGTGGGCAGACTCTCTATGAACAGGTGGTGTTCACCAATCCCAGCTGGGATGCTGAGGTGATCCGGCCCCTACCCATGGACAGCAGTGCCTATTCCTTCACGGCCTTTGTGGGAGTCCCTGCCGTCGAGTTCTCCTTTATGGAGGACGACCAGGCCTACCCATTCCTGCACACAAAGGAGGACACTTATGAGAACCTGCATAAGGTGCTGCAAGGCCGCCTGCCCGCCGTGGCCCAGGCCGTGGCCCAGCTCGCAGGGCAGCTCCTCATCCGGCTCAGCCACGATCGCCTGCTGCCCCTCGACTTCGGCCGCTACGGGGACGTCGTCCTCAGGCACATCGGGAACCTCAACGAGTTCTCTGGGGACCTCAAGGCCCGCGGGCTGACCCTGCAGTGGGTGTACTCGGCGCGGGGGGACTACATCCGGGCGGCGGAAAAGCTGCGGCAGGAGATCTACAGCTCGGAGGAGAGAGACGAGCGACTGACACGCATGTACAACGTGCGCATAATGCGGGTGGAGTTCTACTTCCTTTCCCAGTACGTGTCGCCAGCCGACTCCCCGTTCCGCCACATCTTCATGGGCCGTGGAGACCACACGCTGGGCGCCCTGCTGGACCACCTGCGGCTGCTGCGCTCCAACAGCTCCGGGACCCCCGGGGCCACCTCCTCCACTGGCTTCCAGGAGAGCCGTTTCCGGCGTCAGCTAGCCCTGCTCACCTGGACGCTGCAAGGGGCAGCCAATGCGCTTAGCGGGGATGTCTGGAACATTGATAACAACTTCTGANOV17e, CG52534-04SEQ ID NO: 466801 aaMW at 88754.1kDProtein SequenceMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPEPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRGSCQACGDSVLVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTQDIPAALSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPAQPISADIASRLLRKLKGPVAPQEWQGSLLGSPYHLGPGPRLRLVVNNHRTSTPINNIFGCIEGRSEPDHYVVIGAQRDAWGPGAAKSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWDGGDFGSVGSTEWLEGYLSVLHLKAVVYVSLDNAVLGDDKFHAKTSPLLTSLIESVLKQVDSPNHSGQTLYEQVVFTNPSWDAEVIRPLPMDSSAYSFTAFVGVPAVEFSFMEDDQAYPFLHTKEDTYENLHKVLQGRLPAVAQAVAQLAGQLLIRLSHDRLLPLDFGRYGDVVLRHIGNLNEFSGDLKARGLTLQWVYSARGDYIRAAEKLRQEIYSSEERDERLTRMYNVRIMRVEFYFLSQYVSPADSPFRHIFMGRGDHTLGALLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLALLTWTLQGAANALSGDVWNIDNNFNOV17f, CG52534-05SEQ ID NO: 4672531 bpDNA SequenceORF Start: ATG at 65ORF Stop: TGA at 2468CTGCAGGCTTCAGGAGGGGTACTCTGCCTTCTGGACACTGCAGGCTTCAGGAGGGGACACAAGCATGGAGCGGCTTTGGGGTCTATTCCAGAGAGCGCAACAACTGTCCCCAAGATCCTCTCAGACCGTCTACCAGCGTGTGGAAGGCCCCCGGAAAGGGCACCTGGAGGAGGAAGAGGAAGACGGGGAGGAGGGGGCGGAGACATTGGCCCACTTCTGCCCCATGGAGCTGAGGGGCCCTGAGCCCCTGGGCTCTAGACCCAGGCAGCCAAACCTCATTCCCTGGGCGGCAGCAGGACGGAGGGCTGCCCCCTACCTGGTCCTGACGGCCCTGCTGATCTTCACTGGGGCCTTCCTACTGGGCTACGTCGCCTTCCGAGGGTCCTGCCAGGCGTGCGGAGACTCTGTGTTGGTGGTCAGTGAGGATGTCAACTATGAGCCTGACCTGGATTTCCACCAGGGCAGACTCTACTGGAGCGACCTCCAGGCCATGTTCCTGCAGTTCCTGGGGGAGGGGCGCCTGGAGGACACCATCAGGCAAACCAGCCTTCGGGAACGGGTGGCAGGCTCGGCCGGGATGGCCGCTCTGACTCAGGACATTCGCGCGGCGCTCTCCTGCCAGAAGCTGGACCACGTGTGGACCGACACGCACTACGTGGGGCTGCAATTCCCGGATCCGGCTCACCCCAACACCCTGCACTGGGTCGATGAGGCCGGGAAGGTCGGAGAGCAGCTGCCGCTGGAGGACCCTGACGTCTACTGCCCCTACAGCGCCATCGGCAACGTCACGGGAGAGCTGGTGTACGCCCACTACGGGCGGCCCGAAGACTTGCAGGACCTGCGGGCCAGGGGCGTGGATCCAGTGGGCCGCCTGCTGCTGGTGCGCGTGGGGGTGATCAGCTTCGCCCAGAAGGTGACCAATGCTCAGGACTTCGGGGCTCAAGGAGTGCTCATATACCCAGAGCCAGCGGACTTCTCCCAGGACCCACCCAAGCCAAGCCTGTCCAGCCAGCAGGCAGTGTATGGACATGTGCACCTGGGAACTGGGGACCCCTACACACCTGGCTTCCCTTCCTTCAATCAAACCCAGTTCCCTCCAGTTGCATCATCAGGCCTTCCCAGCATCCCAGCCCAGCCCATCAGTGCAGACATTGCCTCCCGCCTGCTGAGGAAGCTCAAAGGCCCTGTGGCCCCCCAAGAATGGCAGGGGAGCCTCCTAGGCTCCCCTTATCACCTGGGCCCCGGGCCACGACTGCGGCTAGTGGTCAACAATCACAGGACCTCCACCCCCATCAACAACATCTTCGGCTGCATCGAAGGCCGCTCAGAGCCAGATCACTACGTTGTCATCGGGGCCCAGAGGGATGCATGGGGCCCAGGAGCAGCTAAATCCGCTGTGGGGACGGCTATACTCCTGGAGCTGGTGCGGACCTTTTCCTCCATGGTGAGCAACGGCTTCCGGCCCCGCAGAAGTCTCCTCTTCATCAGCTGGGACGGTGGTGACTTTGGAAGCGTGGGCTCCACGGAGTGGCTAGAGGGCTACCTCAGCGTGCTGCACCTCAAAGCCGTAGTGTACGTGAGCCTGGACAACGCAGTGCTGGGGGATGACAAGTTTCATGCCAAGACCAGCCCCCTTCTGACAAGTCTCATTGAGAGTGTCCTGAAGCAGGTGGATTCTCCCAACCACAGTGGGCAGACTCTCTATGAACAGGTGGTGTTCACCAATCCCAGCTGGGATGCTGAGGTGATCCGGCCCCTACCCATGGACAGCAGTGCCTATTCCTTCACGGCCTTTGTGGGAGTCCCTGCCGTCGAGTTCTCCTTTATGGAGGACGACCAGGCCTACCCATTCCTGCACACAAAGGAGGACACTTATGAGAACCTGCATAAGGTGCTGCAAGGCCGCCTGCCCGCTGTGGCCCAGGCCGTGGCCCAGCCCGCAGGGCAGCTCCTCATCCGGCTCAGCCACGATCGCCTGCTGCCCCTCGACTTCGGCCGCTACGGGGACGTCGTCCTCAGGCACATCGGGAACCTCAACGAGTTCTCTGGGGACCTCAAGGCCCGCGGGCTGACCCTGCAGTGGGTGTACTCGGCGCGGGGGGACTACATCCGGGCGGCGGAAAAGCTGCGGCAGGAGATCTACAGCTCGGAGGAGAGAGACGAGCGACTGACACGCATGTACAACGTGCGCATAATGCGGGTGGAGTTCTACTTCCTTTCCCAGTACGTGTCGCCAGCCGACTCCCCGTTCCGCCACATCTTCATGGGCCGTGGAGACCACACGCTGGGCGCCCTGCTGGACCACCTGCGGCTGCTGCGCTCCAACAGCTCCGGGACCCCCGGGGCCACCTCCTCCACTGGCTTCCAGGAGAGCCGTTTCCGGCGTCAGCTAGCCCTGCTCACCTGGACGCTGCAAGGGGCAGCCAATGCGCTTAGCGGGGATGTCTGGAACATTGATAACAACTTCTGAGGCCCTGGGGATCCTCACATCCCCGTCCCCCAGTCAAGAGCTCCTCTGCACCTCGACTTGANOV17f, CG52534-05SEQ ID NO: 468801 aaMW at 88685.0kDProtein SequenceMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPEPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRGSCQACGDSVLVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTQDIRAALSCQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPAQPISADIASRLLRKLKGPVAPQEWQGSLLGSPYHLGPGPRLRLVVNNHRTSTPINNIFGCIEGRSEPDHYVVIGAQRDAWGPGAAKSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWDGGDFGSVGSTEWLEGYLSVLHLKAVVYVSLDNAVLGDDKFHAKTSPLLTSLIESVLKQVDSPNHSGQTLYEQVVFTNPSWDAEVIRPLPMDSSAYSFTAFVGVPAVEFSFMEDDQAYPFLHTKEDTYENLHKVLQGRLPAVAQAVAQPAGQLLIRLSHDRLLPLDFGRYGDVVLRHIGNLNEFSGDLKARGLTLQWVYSARGDYIRAAEKLRQEIYSSEERDEPLTRMYNVRIMRVEFYFLSQYVSPADSPFRHIFMGRGDHTLGALLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLALLTWTLQGAANALSGDVWNIDNNF


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 17B.

TABLE 17BComparison of the NOV17 protein sequences.NOV17aMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPENOV17bMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPENOV17c--------MHYYRYSNAKVSCWYKYLLFSYNIIFWLA---G-------VVFLGVGLWAWSNOV17d--------MHYYRYSNAKVSCWYKYLLFSYNIIFWLA---G-------VVFLGVGLWAWSNOV17eMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPENOV17fMERLWGLFQRAQQLSPRSSQTVYQRVEGPRKGHLEEEEEDGEEGAETLAHFCPMELRGPENOV17aPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRG-SCQA---CGDSVLNOV17bPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRG-SCQA---CGDSVLNOV17cEKGVLSDLTKVT--RMHGIDPVVLVLMVGVVMFTLGFAGCVGALRENICLLNFFCGTIVLNOV17dEKGVLSDLTKVT--RMHGIDPVVLVLMVGVVMFTLGFAGCVGALRENICLLNFFCGTIVLNOV17ePLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRG-SCQA---CGDSVLNOV17fPLGSRPRQPNLIPWAAAGRRAAPYLVLTALLIFTGAFLLGYVAFRG-SCQA---CGDSVLNOV17aVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTNOV17bVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAAADNOV17cIFFLELAVA-VLAFLF--QDW--VRDRFREFF-----ESNIK--SYRDDIDLQNLIDSLQNOV17dIFFLELAVA-VLAFLF--QDW--VRDRFREFF-----ESNIK--SYRDDIDLQNLIDSLQNOV17eVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTNOV17fVVSEDVNYEPDLDFHQGRLYWSDLQAMFLQFLGEGRLEDTIRQTSLRERVAGSAGMAALTNOV17aQDIRAALSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSNOV17bSGHSRGLSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSNOV17cKANQCCGAYGPED--WDLNVYFNCSGASYSREKCGVPFSCCVPDPVQKVV---NTQCGYDNOV17dKANQCCGAYGPED--WDLNVYFNCSGASYSREKCGVPFSCCVPDPAQKVV---NTQCGYDNOV17eQDIRAALSRQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSNOV17fQDIRAALSCQKLDHVWTDTHYVGLQFPDPAHPNTLHWVDEAGKVGEQLPLEDPDVYCPYSNOV17aAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLNOV17bAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLNOV17cVRIQLKSKWDESIFTKGC-IQALESW--LP--RNIYIVAGV--FIA-ISLLQIFG-----NOV17dVRIQLKSKWDESIFTKGC-IQALESW--LP--RNIYIVAGV--FIA-ISLLQIFG-----NOV17eAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLNOV17fAIGNVTGELVYAHYGRPEDLQDLRARGVDPVGRLLLVRVGVISFAQKVTNAQDFGAQGVLNOV17aIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPANOV17bIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPRNOV17cIFLARTLIS-D--IEAVK--A---GH-HF-------------------------------NOV17dIFLARTLIS-D--IEAVK--A---GH-HF-------------------------------NOV17eIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPANOV17fIYPEPADFSQDPPKPSLSSQQAVYGHVHLGTGDPYTPGFPSFNQTQFPPVASSGLPSIPANOV17aQPISADIASRLLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLALLTWTLQGAANALSGDNOV17bR------ALWSLKKKKGP---------------G----PRLRLVVNNHRTSTPINNIFGCNOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eQPISADIASRLLRKLKGPVAPQEWQGSLLGSPYHLGPGPRLRLVVNNHRTSTPINNIFGCNOV17fQPISADIASRLLRKLKGPVAPQEWQGSLLGSPYHLGPGPRLRLVVNNHRTSTPINNIFGCNOV17aVWNIDNNF----------------------------------------------------NOV17bIEGRSEPDHYVVIGAQRDAWGPGAAKSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWNOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eIEGRSEPDHYVVIGAQRDAWGPGAAXSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWNOV17fIEGRSEPDHYVVIGAQRDAWGPGAAKSAVGTAILLELVRTFSSMVSNGFRPRRSLLFISWNOV17a------------------------------------------------------------NOV17bDGGDFGSVGSTEWXRGLPQRAAPQSRSVREPGQRSEGG----------------------NOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eDGGDFGSVGSTEWLEGYLSVLHLKAVVYVSLDNAVLGDDKFHAKTSPLLTSLIESVLKQVNOV17fDGGDFGSVGSTEWLEGYLSVLHLKAVVYVSLDNAVLGDDKFHAKTSPLLTSLIESVLKQVNOV17a------------------------------------------------------------NOV17b------------------------------------------------------------NOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eDSPNHSGQTLYEQVVFTNPSWDAEVIRPLPMDSSAYSFTAFVGVPAVEFSFMEDDQAYPFNOV17fDSPNHSGQTLYEQVVFTNPSWDAEVIRPLPMDSSAYSFTAFVGVPAVEFSFMEDDQAYPFNOV17a------------------------------------------------------------NOV17b------------------------------------------------------------NOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eLHTKEDTYENLHKVLQGRLPAVAQAVAQLAGQLLIRLSHDRLLPLDFGRYGDVVLRHIGNNOV17fLHTKEDTYENLHKVLQGRLPAVAQAVAQPAGQLLIRLSHDRLLPLDFGRYGDVVLRHIGNNOV17a------------------------------------------------------------NOV17b------------------------------------------------------------NOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eLNEFSGDLKARGLTLQWVYSARGDYIRAAEKLRQEIYSSEERDERLTRMYNVRIMRVEFYNOV17fLNEFSGDLKARGLTLQWVYSARGDYIRAAEKLRQEIYSSEERDERLTRMYNVRIMRVEFYNOV17a------------------------------------------------------------NOV17b------------------------------------------------------------NOV17c------------------------------------------------------------NOV17d------------------------------------------------------------NOV17eFLSQYVSPADSPFRHIFMGRGDHTLGALLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLNOV17fFLSQYVSPADSPFRHIFMGRGDHTLGALLDHLRLLRSNSSGTPGATSSTGFQESRFRRQLNOV17a-------------------------NOV17b-------------------------NOV17c-------------------------NOV17d-------------------------NOV17eALLTWTLQGAANALSGDVWNIDNNFNOV17fALLTWTLQGAANALSGDVWNIDNNFNOV17a(SEQ ID NO: 458)NOV17b(SEQ ID NO: 460)NOV17c(SEQ ID NO: 462)NOV17d(SEQ ID NO: 464)NOV17e(SEQ ID NO: 466)NOV17f(SEQ ID NO: 468)


Further analysis of the NOV17a protein yielded the following properties shown in Table 17C.

TABLE 17CProtein Sequence Properties NOV17aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 2; neg. chg 1H-region:length 6; peak value −7.91PSG score:−12.31GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −6.46possible cleavage site: between 16 and 17>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 86-102−5.68PERIPHERALLikelihood = 1.54 (at 264)ALOM score: −5.68 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 93Charge difference: −3.0 C(0.0)-N(3.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 86)MITDISC:discrimination of mitochondrial targeting seqR content: 4Hyd Moment(75):8.36Hyd Moment(95):11.20G content:1D/E content: 2S/T content:4Score: −1.76Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 35 QRV|EGNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.0%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: ERLWnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):47.8%: mitochondrial26.1%: cytoplasmic 8.7%: Golgi 4.3%: vacuolar 4.3%: vesicles of secretory system 4.3%: nuclear 4.3%: endoplasmic reticulum>> prediction for CG52534-06 is mit (k = 23)


A search of the NOV17a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 17D.

TABLE 17DGeneseq Results for NOV17aNOV17aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAY96211Transferrin receptor-like (TfR2) protein -1 . . . 372369/372 (99%)0.0Homo sapiens, 801 aa.1 . . . 372370/372 (99%)[WO200027874-A2, 18-MAY-2000]ABB11600Human transferrin receptor homologue,1 . . . 372366/372 (98%)0.0SEQ ID NO: 1970 - Homo sapiens, 8021 . . . 372367/372 (98%)aa. [WO200157188-A2, 09-AUG-2001]AAB93873Human protein sequence SEQ ID1 . . . 158158/158 (100%)9e−90NO: 13773 - Homo sapiens, 158 aa.1 . . . 158158/158 (100%)[EP1074617-A2, 07-FEB-2001]ABP56714Human CD71 protein SEQ ID NO: 28 -84 . . . 371 112/299 (37%)1e−42Homo sapiens, 760 aa.68 . . . 349 159/299 (52%)[WO2002100336-A2, 19-DEC-2002]ABU04022Human expressed protein tag (EPT)84 . . . 371 112/299 (37%)1e−42#688 - Homo sapiens, 760 aa.68 . . . 349 159/299 (52%)[WO200278524-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV17a protein was found to have homology to the proteins shown in the BLASTP data in Table 17E.

TABLE 17EPublic BLASTP Results for NOV17aNOV17aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9UP52Transferrin receptor protein 2 (TfR2) - 1 . . . 372369/372 (99%)0.0Homo sapiens (Human), 801 aa. 1 . . . 372370/372 (99%)Q9JKX3Transferrin receptor protein 2 (TfR2) - 1 . . . 371293/371 (78%) e−166Mus musculus (Mouse), 798 aa. 1 . . . 366313/371 (83%)Q07891Transferrin receptor protein 1 (TfR1)87 . . . 371112/296 (37%)6e−45(TR) (TfR) (Trfr) - Cricetulus griseus71 . . . 346160/296 (53%)(Chinese hamster), 757 aa.P02786Transferrin receptor protein 1 (TfR1)84 . . . 371112/299 (37%)3e−42(TR) (TfR) (Trfr) (CD71 antigen) (T9)68 . . . 349159/299 (52%)(p90) - Homo sapiens (Human), 760 aa.Q8C872Transferrin receptor - Mus musculus84 . . . 367107/292 (36%)4e−42(Mouse), 515 aa.68 . . . 347152/292 (51%)


PFam analysis predicts that the NOV17a protein contains the domains shown in the Table 17F.


Table 17F. Domain Analysis of NOV17a

TABLE 17FDomain Analysis of NOV17aIdentities/SimilaritiesPfamNOV17afor theExpectDomainMatch RegionMatched RegionValuePA236 . . . 33536/112 (32%)8.7e−1470/112 (62%)TFR_dimer247 . . . 41634/188 (18%)0.45121/188 (64%) 


Example 18

The NOV18 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 18A.

TABLE 18ANOV18 Sequence AnalysisNOV18a, CG52979-03SEQ ID NO: 469458 bpDNA SequenceORF Start: ATG at 61Stop: TAG at 343TCTTCATTCTTTCCGCCATCTTGATTCTTTCTCACTGACCAAGACTCAGCCGTGGGAAATATGAGTGAGCTTGTAAGAACAACATCCCAATCCTCAGAAAGAGGAAATGACCAAGAGTCTTCCCAGCCAGTTGGATCTGTGATTGTCCAGGAGCCCACTGAGGAAAAGCGTCAAGAAGAGGAACCACCAACTGATAATCAGGGGTGATGTCAGGGAGGGTATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGGTTTCAAGCAAGACAAATGAAGACTGAAACCAAGAACGTTATTCTTAATCTGGAAATTTGACTGATAATATTCTCTTAATAAAGTTTTAAGTTTTCTGCAAAGAATCCTTAAANOV18a, CG52979-03Protein SequenceSEQ ID NO: 47094 aaMW at 10341.1kDMSELVRTTSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQPNOV18b, CG52979-01SEQ ID NO: 471458 bpDNA SequenceORF Start: ATG at 61ORF Stop: TAG at 343TCTTCATTCTTTCCGCCATCTTGATTCTTTCTCACTGACCAAAGACTCAGCCGTGGGAAAATATGTGAGCTTGTAAGAGCAAGATCCCAATCCTCAGAAAGAGGAAATGACCAAGAGTCTTCCCAGCCGGTTGGATCTGTGATTGTCCAGGAGCCCACTGAGGAAAAACGTCAAGAAGAGGAACCACCAACTGATAATCAGGGGCCTGACATGGAAGCTTTTCAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGTATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGGTTTCAAGCAAGACAAATGAAGACTGAAACCAAGAACGTTATTCTTAATCTGGAAATTTGACTGATAATATTCTCTTAATAAAGTTTTAAGTTTTCTGCAAAGAATCCTTAAANOV18b, CG52979-01Protein SequenceSEQ ID NO: 47294 aaMW at 10366.1kDMSELVRTTSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQPNOV18c, CG52979-02SEQ ID NO: 473336 bpDNA SequenceORF Start: at 1ORF Stop: at 112TGGTTGCGCATCACCTGCTTCCAGCACTTTAGTGAGATCAAAAGTGGGCATAATACCCTCCCTGACATCAGGACCATCTCCAGGCTCATCCTCTATCTTAAGCAGAGCCAGTTCCTGTTGAAAAGCTTCCATGTCAGGCCCTTGAAAAGCAGGCACTGCTTGATTTTCAATCTCCCCACTAGGTGCAATACCCTGATTATCAGTTGGTGGTTCCTCTTCTTGACGTTTTTCCTCAGTGGGCTCCTGGACAATCACAGATCCAACCGGCTGGGAAGACTCTTGGTCATTTCCTCTTTCTGAGGATTGGGATCTTGCTCTTACAAGCTCACTCATGGTNOV18c, CG52979-02Protein SequenceSEQ ID NO: 47437 aaMW at 4444.1kDWLRITCFQHFSEIKSGHNTLPDIRTISRLILYLKQSQ


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 18B.

TABLE 18BComparison of the NOV18 protein sequences.NOV18aMSELVRTTSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGPDMEAFQQELALNOV18bMSELVRARSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGPDMEAFQQELALNOV18c----------------------------WLRITCFQHFSEIKSGHNTLPDIRTISRLILYNOV18aLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQPNOV18bLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQPNOV18cLKQSQ-----------------------------NOV18a (SEQ ID NO: 470)NOV18b (SEQ ID NO: 472)NOV18c (SEQ ID NO: 474)


Further analysis of the NOV18a protein yielded the following properties shown in Table 18C.

TABLE 18CProtein Sequence Properties NOV18aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos. chg 1; neg. chg 1H-region:length 6; peak value −10.69PSG score:−15.09GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −14.03possible cleavage site: between 14 and 15>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 10.87 (at 76)ALOM score: 10.87 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content: 1Hyd Moment(75):5.19Hyd Moment(95):12.09G content:0D/E content: 2S/T content:6Score: −3.75Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 7.4%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):78.3%: nuclear21.7%: mitochondrial>> prediction for CG52979-03 is nuc (k = 23)


A search of the NOV18a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 18D.

TABLE 18DGeneseq Results for NOV18aNOV18aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE08580Human NOV1 protein - Homo sapiens,1 . . . 9492/94 (97%)1e−4794 aa. [WO200161009-A2, 23-AUG-1 . . . 9492/94 (97%)2001]AAE08593Protein encoded by pCR2.1-83350421-1 . . . 9492/111 (82%)2e−44S747-3A insert - Homo sapiens, 111 aa. 1 . . . 11192/111 (82%)[WO200161009-A2, 23-AUG-2001]AAE08582Human NOV3 protein - Homo sapiens,1 . . . 9492/111 (82%)2e−44111 aa. [WO200161009-A2, 23-AUG- 1 . . . 11192/111 (82%)2001]ABJ19255Human cancer/testis antigen - SEQ ID1 . . . 9492/111 (82%)4e−44No 37 - Homo sapiens, 111 aa. 1 . . . 11192/111 (82%)[WO200278526-A2, 10-OCT-2002]AAE08581Human NOV2 protein - Homo sapiens,1 . . . 9492/111 (82%)4e−44111 aa. [WO200161009-A2, 23-AUG- 1 . . . 11192/111 (82%)2001]


In a BLAST search of public sequence databases, the NOV18a protein was found to have homology to the proteins shown in the BLASTP data in Table 18E.

TABLE 18EPublic BLASTP Results for NOV18aNOV18aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8WWL9Page-5 protein - Homo sapiens1 . . . 9380/110 (72%)4e−35(Human), 130 aa.21 . . . 12984/110 (75%)Q96GU1Hypothetical protein - Homo sapiens1 . . . 9380/110 (72%)4e−35(Human), 110 aa. 1 . . . 10984/110 (75%)O60829G antigen family C 1 protein (Prostate-6 . . . 9439/104 (37%)1e−06associated gene protein 4) (PAGE-4) 4 . . . 10247/104 (44%)(PAGE-1) (JM27) (GAGE-9) - Homosapiens (Human), 102 aa.Q8WTP9Placenta-specific gene 6 protein (XAGE-31 . . . 94 31/82 (37%)4e−063 protein) (pp9012) - Homo sapiens28 . . . 10943/82 (51%)(Human), 111 aa.Q8WWM1XAGE-5 protein - Homo sapiens21 . . . 94 32/92 (34%)7e−06(Human), 108 aa.17 . . . 10649/92 (52%)


PFam analysis predicts that the NOV18a protein contains the domains shown in the Table 18F.

TABLE 18FDomain Analysis of NOV18aPfamNOV18a Match RegionIdentities/ExpectDomainSimilarities forValuethe MatchedRegion


Example 19.

The NOV19 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 19A.

TABLE 19ANOV19 Sequence AnalysisNOV19a, CG52988-02SEQ ID NO: 475501 bpDNA SequenceORF Start: ATG at 25ORF Stop: TAG at 358GGGAAGAGACCATGTGTGGGAAATATGAGTGAGCTTGTAAGAACAAGATCCCAATCCTCAGAAAGAGGAAATGACCAAGAGTCTTCCCAGCCGGTTGGATCTGTGATTGTCCAGGAGCCCACTGAGGAAAAACGTCAAGAAGAGGAACCACCAACTGATAATCAGGGTATTGCACCTAGTGGGGAGATTGAAAATCAAGCAGTGCCTGCTTTTCAAGGGCCTGACATGGAAGCTTTTCAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGTATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGGTTTCAAGCAAGACAAATGAAGACTGAAACCAAGAACGTTATTCTTAATCTGGAAATTTGACTGATAATATTCTCTTAATAAAGTTTTAAGTTTTCTGCAAAGAATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGNOV19a, CG52988-02SEQ ID NO: 476111 aaMW at 12106.0kDProtein SequenceMSELVRTRSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGIAPSGEIENQAVPAFQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQPNOV19b, CG52988-03SEQ ID NO: 477374 bpDNA SequenceORF Start: ATG at 23ORF Stop: TAG at 356GAAGAGACCATGTGTGGGAAATATGAGTGAGCTTGTAAGAGCAAGATCCCAATCCTCAGAAAGAGGAAATGACCAAGAGTCTTCCCAGCCGGTTGGATCTGTGATTGTCCAGGAGCCCACTGAGGAAAACGTCAGAAGAGGAACCACCAACTGATAATCAGGGTATTGCACCTAGTGGGGAGATTGAAAATCAAGCAGTGCCTGCTTTTCAAGGGCCTGACATGGAAGCTTTTCAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGTATTATGCCCACTTTTGATCTCACTAA~GTGCTGGAAGCAIGGTGATGCGCAACCATAQGTTTCAAGCAAGACAANOV19b, CG52988-03SEQ ID NO: 478111 aaMW at 12076 0kDProtein SequenceMSELVRARSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGIAPSGEIENQAVPAFQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTEDLTKVLEAGDAQPNOV19c, CG52988-04SEQ ID NO: 4791337 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 334ATGAGTGAGCATGTGAGAACAACATCCCAATCCTCAGAAGAGGAAATGACCAAGAGTCTTCCCAGCCAGTTGGATCTGTGATTGTCCAGGAGCCCACTGAGGAAAAACGTCAAGAAGAGGAACCACCAACTGATAATCAGGGTATTGCACCTAGTGGGGAGATCGAAAATGAAGGAGCACCTGCCGTTCAAGGGCCTGACATGGAAGCTTTTCAAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGGATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGTNOV19c, CG52988-04SEQ ID NO: 480111 aMW at 11985.8kDProtein SequenceMSEAAAATTSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGIAPSGEIENEGAPAVQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQPNOV19d, CG52988-06SEQ ID NO: 481344 bpDNA SequenceORF Start: ATG at 9ORF Stop: TAG at 342GTGGAATATGATTGAGCATGTGAGAACAATATCCCAATCCTCAGAAGAGGAAATGACCAAAGAGTCTAACTGATAATCAGGGTATTGCACCTAGTGGGGAGATCGAAAATGAAGGAGCACCTGCCGTTCAAGGGCCTGACATGGAAGCTTTTCAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGGATTATGCCCACTTTTGATCTCACTAGAGTGCTGGAAGCAGGTGATGCGCAACCATAGNOV19d, CG52988-06SEQ ID NO: 482111 aaMW at 12061 .0kDProtein SequenceMIEHVRTSQSSERGNDQESSHPVGSVIVQEPTEEKRQEEEPPTDNQGIAPSGEIENEGAPAVQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTRVLEAGDAQPlNOV19e, CG52988-07SEQ ID NO: 4831343 bpDNA SequenceORF Start: ATG at 8ORF Stop: TAG at 341GGGAAATATGAGTGAGCATGTGAGAACAAGATCCCAATCCTCAGAAAGAGGAAATGACCAAGAGTCTTACTGATAATCAGGGTATTGCGCCTAGTGGGGAGATCGAAAATGAAGGAGCACCTGCCGTTCAAGGGCCTGACATGGAAGCTTTTCAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGGATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGNOV19e, CG52988-07SEQ ID NO: 484111 aaMW at 12040.9kDProtein SequenceMSEHVRTRSQSSERGNDQESSQPVGSVTVQEPTEEKRQEEEPTDNQGIAGEIENEGAPAVQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQNOV19f, CG52988-01SEQ ID NO: 485475 bpDNA SequenceORF Start: ATG at 25ORF Stop: TAG at 358AAATGACCAAGAGTCTTCCCAGCCAGTTGGATCTGTGATTGTCCAGGAGCCCACTGAGGAAAAAAACGTCAAGAAGAGGAACCACCAACTGATAATCAGGGTATTGCACCTAGTGGGGAGATCGAATGAAAGGAGCACCTGCCGTTCAAGGGCCTGACATGGAAGCTTTTCACAGGAAACTGGCTCTGCTTAAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGGATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGGTTTCAAGCAGACATGAAGACTGAACCAGAACGTTATTCTTA14TCTGGAAATTTGACTGATAATATTCTCTTAATAGTTTTAGTTTTCTGCAGANOV19f,CG52988-01SEQ ID NO: 486111 aaMW at 12040.9kDProtein SequenceMSEHVRTRSQSSERGNDQESSQPVGSVIVQEPTEEKRQEEEPPTDNQGIAPSGEIENEGAPAVQGPDMIEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKvLEAGDAQPNOV19g, CG52988-05SEQ ID NO: 4871344 bpDNA SequenceORF Start: ATG at 9ORF Stop: TAG at 342TGGGAAATATGAGTGAGCATGTGAGAACAACATCCCAATCCTCAGAAAGAGGAAATGACCAAGAGTCTCTGACATGGAAGCTTTTCAACAGGAACTGGCTCTGCTTAAGATAGAGGATGAGCCTGGAGATGGTCCTGATGTCAGGGAGGGGATTATGCCCACTTTTGATCTCACTAAAGTGCTGGAAGCAGGTGATGCGCAACCATAGNOV19g, CG52988-05SEQ ID NO: 488111 aaaMW at 12027.9kDProtein SequenceMSEHVRTTSQSSERGNDQESSQPVVSVIVQEPTEEKRQEEEPPTDNQGIApSGEIENEGAPAVQGPDMEAFQQELALLKIEDEPGDGPDVREGIMPTFDLTKVLEAGDAQP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 19B.

TABLE 19BComparison of the NOV19 protein sequences.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(SEQ ID NO: 476)NOV19b(SEQ ID NO: 478)NOV19c(SEQ ID NO: 480)NOV19d(SEQ ID NO: 482)NOV19e(SEQ ID NO: 484)NOV19f(SEQ ID NO: 486)NOV19g(SEQ ID NO: 488)


Further analysis of the NOV19a protein yielded the following properties shown in Table 19C.

TABLE 19CProtein Sequence Properties NOV19aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 8; pos. chg 2; neg. chg 1H-region:length 4; peak value −13.93PSG score:−18.33GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −15.73possible cleavage site: between 14 and 15>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 10.87 (at 93)ALOM score: 10.87 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):8.54Hyd Moment(95):6.16G content:0D/E content:2S/T content:5Score: −3.37Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 7.2%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):69.6%: nuclear26.1%: mitochondrial 4.3%: cytoplasmic>> prediction for CG52988-02 is nuc (k = 23)


A search of the NOV19a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 19D.

TABLE 19DGeneseq Results for NOV19aNOV19aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE08593Protein encoded by pCR2.1-83350421-1 . . . 111110/111 (99%)3e−58S747-3A insert - Homo sapiens, 1111 . . . 111110/111 (99%)aa. [WO200161009-A2, 23-AUG-2001]AAE08582Human NOV3 protein - Homo sapiens,1 . . . 111110/111 (99%)3e−58111 aa. [WO200161009-A2, 23-AUG-1 . . . 111110/111 (99%)2001]ABJ19255Human cancer/testis antigen - SEQ ID1 . . . 111106/111 (95%)6e−56No 37 - Homo sapiens, 111 aa.1 . . . 111107/111 (95%)[WO200278526-A2, 10-OCT-2002]AAE08581Human NOV2 protein - Homo sapiens,1 . . . 111106/111 (95%)6e−56111 aa. [WO200161009-A2, 23-AUG-1 . . . 111107/111 (95%)2001]ABJ19247Human cancer/testis antigen - SEQ ID1 . . . 11093/110 (84%)7e−47No 21 - Homo sapiens, 110 aa.1 . . . 10999/110 (89%)[WO200278526-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV19a protein was found to have homology to the proteins shown in the BLASTP data in Table 19E.

TABLE 19EPublic BLASTP Results for NOV19aNOV19aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8WWL9Page-5 protein - Homo sapiens 1 . . . 11093/110(84%)2e−46(Human), 130 aa.21 . . . 12999/110(89%)Q96GU1Hypothetical protein - Homo sapiens 1 . . . 11093/110(84%)2e−46(Human), 110 aa. 1 . . . 10999/110(89%)O60829G antigen family C 1 protein (Prostate- 6 . . . 11145/106(42%)5e−11associated gene protein 4) (PAGE-4) 4 . . . 10253/106(49%)(PAGE-1) (JM27) (GAGE-9) - Homosapiens (Human), 102 aa.Q8WTP9Placenta-specific gene 6 protein (XAGE- 8 . . . 11142/108(38%)3e−103 protein) (pp9012) - Homo sapiens 4 . . . 10962/108(56%)(Human), 111 aa.Q8WWM1XAGE-5 protein - Homo sapiens21 . . . 11136/92(39%)5e−09(Human), 108 aa.17 . . . 10655/92(59%)


PFam analysis predicts that the NOV19a protein contains the domains shown in the Table 19F.

TABLE 19FDomain Analysis of NOV19aPfamNOV19aIdentities/ExpectDomainMatch RegionSimilaritiesValuefor theMatched Region


Example 20

The NOV20 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 20A.

TABLE 20ANOV20 Sequence AnalysisNOV20a, CG53449-04SEQ ID NO: 4892165 bpDNA SequenceORF Start: ATG at 153ORF Stop: TGA at 1194AGAACAGCGCAGTTTGCCCTCCGCTCACGCAGAGCCTCTCCGTGGCCTCCGCACCTTGAGCATTAGGCCAGTTCTCCTCTTCTCTCTAATCCATCCGTCACCTCTCCTGTCATCCGTTTCCATGCCGTGAGGTCCATTCACAGAACACATCCATGGCTCTCATGCTCAGTTTGGTTCTGAGTCTCCTCAAGCTGGGATCAGGGCTTCCTGTCTCCTAAGACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCTGAGGCTGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCACAGCGAAGTGGAAAGGTCCACAAGGACAGGATTTGTCCACAGACTCCAGGACAAACAGAGACATGCATGGCCTGTTTGATGTGGAGATCTCTCTGACCGTCCAAGAGAACGCCGGGAGCATATCCTGTTCCATGCGGCATGCTCATCTGAGCCGAGAGGTGGAATCCAGGGTACAGATAGGAGATACCTTTTTCGAGCCTATATCGTGGCACCTGGCTACCAAAGTACTGGGAATACTCTGCTGTGGCCTATTTTTTGGCATTGTTGGACTGAAGATTTTCTTCTCCAAATTCCAGTGTAAGCGAGAGAGAGAAGCATGGGCCGGTGCCTTATTCATGGTTCCAGCAGGGACAGGATCAGAGATGCTCCCACATCCAGCTGCTTCTCTTCTTCTAGTCCTAGCCTCCAGGGGCCCAGGCCCAAAAAAGGAAAATCCAGGCGGAACTGGACTGGAGAAGAAAGCACGGACAGGCAGAATTGAGAGACGCCCGGAAACACGCAGTGGAGGTGACTCTGGATCCAGAGACGGCTCACCCGAAGCTCTGCGTTTCTGATCTGAAAACTGTAACCCATAGAAAAGCTCCCCAGGAGGTGCCTCACTCTGAGAAGAGATTTACAAGGAAGAGTGTGGTGGCTTCTCAGAGTTTCCAAGCAGGGAAACATTACTGGGAGGTGGACGGAGGACACAATAAAAGGTGGCGCGTGGGAGTGTGCCGGGAATGGAGAACATTTGTATTTCACATTAAATCCCCGTTTTATCAGCGTCTTCCCCAGGACCCCACCTACAAAAATAGGGGTCTTCCTGGACTATGAGTGTGGGACCATCTCCTTCTTCAACATAAATGACCAGTCCCTTATTTATACCCTGACATGTCGGTTTGAAGGCTTATTGAGGCCCTACATTGAGTATCCGTCCTATAATGAGCAAAATGGAACTCCCATAGTCATCTGCCCAGTCACCCAGGAATCAGAGAAAGAGGCCTCTTGGCAAAGGGCCTCTGCAATCCCAGAGACAAGCAACAGTGAGTCCTCCTCACAGGCAACCACGCCCTTCCTCCCCAGGGGTGAAATGTAGGATGAATCACATCCCACATTCTTCTTTAGGGATATTAAGGTCTCTCTCCCAGATCCAAAGTCCCGCAGCAGCCGGCCAAGGTGGCTTCCAGATGAAGGGGGACTGGCCTGTCCACATGGCTCCATCTGGCTAAGTGATCTTGAAATACCACCTCTCAGGTGAAGAACCGTCAGGAATTCCCATCTCATATCCTAATGGTTTGTTCATTATATTACACTTTCAGTAAAAAAAAAAAAAAAAAAANOV20a, CG53449-04Protein SequenceSEQ ID NO: 490347 aaMW at 38208.5kDMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRISLRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDPDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQCKREREAWAGALFMVPAGTGSEMLPHPAASLLLVLASRGPGPKKENPGGTGLEKKARTGRIERRPETRSGGDSGSRDGSPEALRFNOV20b, CG53449-03SEQ ID NO: 4911969 bpDNA SequenceORF Start: ATG at 95ORF Stop: TAG at 1595AGCATTAGGCCAGTTCTCCTCTTCTCTCTAATCCATCCGTCACCTCTCCTGTCATCCGTTTCCATGCCGTGAGGTCCATTCACAGAACACATCCATGGCTCTCATGCTCAGTTTGGTTCTGAGTCTCCTCAAGCTGGGATCAGGGCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAGACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCTGAGGCTGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCACAGCGAAGTGGAAAGGTCCACAAGGACAGGATTTGTCCACAGACTCCAGGACAAACAGAGACATGCATGGCCTGTTTGATGTGGAGATCTCTCTGACCGTCCAAGAGAACGCCGGGAGCATATCCTGTTCCATGCGGCATGCTCATCTGAGCCGAGAGGTGGAATCCAGGGTACAGATAGGAGATACCTTTTTCGAGCCTATATCGTGGCACCTGGCTACCAAAGTACTGGGAATACTCTGCTGTGGCCTATTTTTTGGCATTGTTGGACTGAAGATTTTCTTCTCCAAATTCCAGTGGAAAATCCAGGCGGAACTGGACTGGAGAAGAAAGCACGGACAGGCAGAATTGAGAGACGCCCGGAAACACGCAGTGGAGGTGACTCTGGATCCAGAGACGGCTCACCCGAAGCTCTGCGTTTCTGATCTGAAPACTGTAACCCATAGAAAAGCTCCCCAGGAGGTGCCTCACTCTGAGAAGAGATTTACAAGGAAGAGTGTGGTGGCTTCTCAGAGTTTCCAAGCAGGGAAACATTACTGGGAGGTGGACGGAGGACACAATAAAAGGTGGCGCGTGGGAGTGTGCCGGGATGATGTGGACAGGAGGAAGGAGTACGTGACTTTGTCTCCCGATCATGGGTACTGGGTCCTCAGACTGAATGGAGAACATTTGTATTTCACATTAAATCCCCGTTTTATCAGCGTCTTCCCCAGGACCCCACCTACAAAAATAGGGGTCTTCCTGGACTATGAGTGTGGGACCATCTCCTTCTTCAACATAAATGACCAGTCCCTTATTTATACCCTGACATGTCGGTTTGAAGGCTTATTGAGGCCCTACATTGAGTATCCGTCCTATAATGAGCAAAATGGAACTCCCATAGTCATCTGCCCAGTCACCCAGGAATCAGAGAACCACGCCCTTCCTCCCCAGGGGTGAAATGTAGGATGAATCACATCCCACATTCTTCTTTAGGGATATTAAGGTCTCTCTCCCAGATCCAAAGTCCCGCAGCAGCCGGCCAAGGTGGCTTCCAGATGAAGGGGGACTGGCCTGTCCACATGGGAGTCAGGTGTCATGGCTGCCCTGAGCTGGGAGGGAAGAAGGCTGACATTACATTTAGTTTGCTCTCACTCCATCTGGCTAAGTGATCTTGAAATACCACCTCTCAGGTGAAGAACCGTCAGGAATTCCCATCTCACAGGCTGTGGTGTAGATTAAGTAGACAAGGAATGTGAATAATGCTTAGATCTTATTGATGACAGAGTGTATCCTAATGGTTTGTTCATTATATTACACTTTCAGTAAAAAAAAAAAANOV20b, CG53449-03Protein SequenceSEQ ID NO: 492500 aaMW at 56747.1kDMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRISLRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQWKIQAELDWRHYWEVDGGHNKRWRVGVCRDDVDRRKEYVTLSPDHGYWVLRLNGEHLYFTLNPRFISVFPRTPPTKIGVFLDYECGTISFFNINDQSLIYTLTCRFEGLLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSESSSQATTPFLPRGEMNOV20c, CG53449-01SEQ ID NO: 493252 bpDNA SequenceORF Start: ATG at 1ORF Stop: end of sequenceATGAACCCGGTACCTCAGATGGAAATGCAGAAATCACCCATGTTCTGCGTCGCTCACTCTGGGAGCTGTAGACCGGAGCTGTTCCTATTCGGCCATCTTGGCTCCTCCCCCTCACTACAAAGGGTTTTGATGATTTATCTTTTGTATGTCTTCCCACAGGGCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAAACCAATGNOV20c, CG53449-01Protein SequenceSEQ ID NO: 49484 aaMW at 9453.1kDMNPVPQMEMQKSPMFCVAHSGSCRPELFLFGHLGSSPSLQRVLMYLLYVFPQGSGRCLGQTSLSRPWWGRTQHSPVSCLLKPMNOV20d, CG53449-02SEQ ID NO: 4951495 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1492ATGAACCCGGTACCTCAGATGGAAATGCAGAAATCACCCATGTTCTGCGTCGCTCACTCTGGGAGCTGTAGACCGGAGCTGTTCCTATTCGGCCATCTTGGCTCCTCCCCCTCACTACAAAGGGTTTTGATGATTTATCTTTTGTATGTCTTCCCACAGGGCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAAACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCGAGGCTGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCGTGCAAGGAGCCAGCGTCGTGTTTGTGCCTTGTACACTCCTGTGTCCACCACTGAATATACTGTTTCTGTTTCAGGGAAAATCCAGGCGGAACTGGGTAAGTATGTGTCATGTCCTGAGCCTCCCACACATGGTTCTCCCGGGTCCCTCCCTGATCCACAGTTTGAGCCTCTGGACGACCCTGGCTGCAGGCTGGACAGGAAGCACCGACTGGAGAAGAAAGCACGGACAGGCAGAATTGAGAGACGCCCGGAAACACGCAGTGGAGGTGACTCTGGATCCAGAGACGGCTCACCCGAAGCTCTGCGTTTCTGATCTGAAAACTGTAACCCATAGAAAAGCTCCCCAGGAGGTGCCTCACTCTGAGAAGAGATTTACAAGGAAGAGTGTGGTGGCTTCTCAGAGTTTCCAAGCAGGGAAACATTACTGGGAGGTGGACGGAGGACACAATAAAAGGTGGCGCGTGGGAGTGTGCCGGGATGATGTGGACAGGAGGAAGGAGTACGTGACTTTGTCTCCCGATCATGGGTACTGGGTCCTCAGACTGAATGGAGAACATTTGTATTTCACATTAAATCCCCGTTTTATCAGCGTCTTCCCCAGGACCCCACCTACAAAAATAGGGGTCTTCCTGGACTATGAGTGTGGGACCATCTCCTTCTTCAACATAAATGACCAGTCCCTTATTTATACCCTGACATGTCGGTTTGAAGGCTTATTGAGGCCCTACATTGAGTATCCGTCCTATAATGAGCAAAATGGAACTCCCATAGTCATCTGCCCAGTCACCCAGGAATCAGAGAAAGAGGCCTCTTGGCAAAGGGCCTCTGCAATCCCAGAGACAAGCAACAGTGAAGTCCTCCTCACAGGCAACCACGCCCTTCCTCCCCAGGGGTGAANOV20d, CG53449-02Protein SequenceSEQ ID NO: 496497 aaMW at 55724.3kDMNPVPQMEMQKSPMFCVAHSGSCRPELFLFGHLGSSPSLQRVLMTYLLYVFPQGSGRCLGQTSLSRPWWGRTQHSPVSCLLKPMQRPWKCGSSGASSLAWSTSTGTGRTSHLCRCHSIKAGQNCEGFYCGGAHLSRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDRDIQLLCQSSGWFPRPVQGASVVFVPCTLLCPPLNILFLFQGKSRRNWVSMCHVLSLPHNVLPGPSLIHSLSLWTTLAAGWTGSTDWRRKHGQAELRDARKHAVEVTLDPETAHPKLCVSDLKTVTHRKAPQEVPHSEKRFTRKSVVASQSFQAGKHYWEVDGGHNKRWRVGVCRDDVDRRKEYVTLSPDHGYWVLRLNGEHLYFTLNPRFISVFPRTPPTKIGVFLDYECGTISFFNINDQSLIYTLTCRFEGLLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSEVLLTGNHALPPQGNOV20e, CG53449-05SEQ ID NO: 497661 bpDNA SequenceORF Start: at 11ORF Stop: at 653CACCAAGCTTCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAGACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCTGAGGCTGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCACAGCGAAGTGGAAAGGTCCACAAGGACAGGATTTGTCCACAGACTCCAGGACAAACAGAGACATGCATGGCCTGTTTGATGTGGAGATCTCTCTGACCGTCCAAGAGAACGCCGGGAGCATATCCTGTTCCATGCGGCATGCTCATCTGAGCCGAGAGGTGGAATCCAGGGTACAGATAGGAGATACCTTTTTCGAGCCTATACTCGAGGGCNOV20e, CG53449-05Protein SequenceSEQ ID NO: 498214 aaMW at 24072.0kDQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRISLRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVQENAGSISCSMRHAHLSREVESRVQIGDTFFEPINOV20f, CG53449-06SEQ ID NO: 4991066 bpDNA SequenceORF Start: ATG at 17ORF Stop: at 1058CACCAAGCTTCCCACCATGGCTCTCATGCTCAGTTTGGTTCTGAGTCTCCTCAAGCTGGGATCAGGGCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAGACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCTGAGGCTGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCACAGCGAAGTGGAAAGGTCCACAAGGACAGGATTTGTCCACAGACTCCAGGACAAACAGAGACATGCATGGCCTGTTTGATGTGGAGATCTCTCTGACCGTCCAAGAGAACGCCGGGAGCATATCCTGTTCCATGCGGCATGCTCATCTGAGCCGAGAGGTGGAATCCAGGGTACAGATAGGAGATACCTTTTTCGAGCCTATATCGTGGCACCTGGCTACCAAAGTACTGGGAATACTCTGCTGTGGCCTATTTTTTGGCATTGTTGGACTGAAGATTTTCTTCTCCAAATTCCAGTGTAAGCGAGAGAGAGAAGCATGGGCCGGTGCCTTATTCATGGTTCCAGCAGGGACAGGATCAGAGATGCTCCCACATCCAGCTGCTTCTCTTCTTCTAGTCCTAGCCTCCAGGGGCCCAGGCCCAAAAAAGGAAAATCCAGGCGGAACTGGACTGGAGAAGAAAGCACGGACAGGCAGAATTGAGAGACGCCCGGAAACACGCAGTGGAGGTGACTCTGGATCCAGAGACGGCTCACCCGAAGCTCTGCGTTTCCTCGAGGGCNOV20f, CG53449-06Protein SequenceSEQ ID NO: 500347 aaMW at 38208.5kDMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRISLRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQCKREREAWAGALFMVPAGTGSEMLPHPAASLLLVLASRGPGPKKENPGGTGLEKKARTGRIERRPETRSGGDSGSRDGSPEALRFSEQ ID NO: 5011969 bpNOV20g, SNP13382434 ofORF Start: ATG at 95ORF Stop: TAG at 1595CG53449-03, DNA SequenceSNP Pos: 396SNP Change: T to CAGCATTAGGCCAGTTCTCCTCTTCTCTCTAATCCATCCGTCACCTCTCCTGTCATCCGTTTCCATGCCGTGAGGTCCATTCACAGAACACATCCATGGCTCTCATGCTCAGTTTGGTTCTGAGTCTCCTCAAGCTGGGATCAGGGCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAGACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCTGAGGCCGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCACAGCGAAGTGGAAAGGTCCACAAGGACAGGATTTGTCCACAGACTCCAGGACAAACAGAGACATGCATGGCCTGTTTGATGTGGAGATCTCTCTGACCGTCCAAGAGAACGCCGGGAGCATATCCTGTTCCATGCGGCATGCTCATCTGAGCCGAGAGGTGGAATCCAGGGTACAGATAGGAGATACCTTTTTCGAGCCTATATCGTGGCACCTGGCTACCAAAGTACTGGGAAATCCAGGCGGAACTGGACTGGAGAAGAAAGCACGGACAGGCAGAATTGAGAGACGCCCGGAAACACGCAGTGGAGGTGACTCTGGATCCAGAGACGGCTCACCCGAAGCTCTGCGTTTCTGATCTGAAAACTGTAACCCATAGAAAAGCTCCCCAGGAGGTGCCTCACTCTGAGAAGAGATTTACAAGGAAGAGTGTGGTGGCTTCTCAGAGTTTCCAAGCAGGGAAACATTACTGGGAGGTGGACGGAGGACACAATAAAAGGTGGCGCGTGGGAGTGTGCCGGGATGATGTGGACAGGAGGAAGGAGTACGTGACTTTGTCTCCCGATCATGGGTACTGGGTCCTCAGACTGAATGGAGAACATTTGTATTTCACATTAAATCCCCGTTTTATCAGCGTCTTCCCCAGGACCCCACCTACAAAAATAGGGGTCTTCCTGGACTATGAGTGTGGGACCATCTCCTTCTTCAACATAAATGACCAGTCCCTTATTTATACCCTGACATGTCGGTTTGAAGGCTTATTGAGGCCCTACATTGAGTATCCGTCCTATAATGAGCAAAATGGAACTCCCATAGTCATCTGCCCAGTCACCCAGGAATCAGAGAAAGAGGCCTCTTGGCAAAGGGCCTCTGCAATCCCAGAGACAAGCAACAGTGAATCCTCCTCACAGGCAACCACGCCCTTCCTCCCCAGGGGTGAAATGTAGGATGAATCACATCCCACATTCTTCTTTAGGGATATTAAGGTCTCTCTCCCAGATCCAAAGTCCCGCAGCAGCCGGCCAAGGTGGCTTCCAGATGAAGGGGGACTGGCCTGTCCACATGGGAGTCAGGTGTCATGGCTGCCCTGAGCTGGGAGGGAAGAAGGCTGACATTACATTTAGTTTGCTCTCACTCCATCTGGCTAAGTGATCTTGAAATACCACCTCTCAGGTGAAGAACCGTCAGGAATTCCCATCTCACAGGCTGTGGTGTAGATTAAGTAGACAAGGAATGTGAATAATGCTTAGATCTTATTGATGACAGAGTGTATCCTAATGGTTTGTTCATTATATTACACTTTCAGTAAAAAAAAAAAANOV20g, SNP13382434 ofSEQ ID NO: 502MW at 56731.1kDCG53449-03, Protein SequenceSNP Pos: 101500 aaSNP Change: Leu to ProMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRISLRPENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQWKIQAELDWRRKNGQAELRDARKHAVEVTLDPETAHPKLCVSDLKTVTHRKAPQEVPHSEKRFTRKSVVASQSFQAGKHYWEVDGGHNKRWRVGVCRDDVDRRKEYVTLSPDHGYWVLRLNGEHLYFTLNPRFISVFPRTPPTKIGVFLDYECGTISFFNINDQSLIYTLTCRFEGLLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSESSSQATTPFLPRGEMSEQ ID NO: 5031969 bpNOV20h, SNP13382441 ofORF Start: ATG at 95ORF Stop: TAG at 1595CG53449-03, DNA SequenceSNP Pos: 1549SNP Change: A to GAGCATTAGGCCAGTTCTCCTCTTCTCTCTAATCCATCCGTCACCTCTCCTGTCATCCGTTTCCATGCCGTGAGGTCCATTCACAGAACACATCCATGGCTCTCATGCTCAGTTTGGTTCTGAGTCTCCTCAAGCTGGGATCAGGGCAGTGGCAGGTGTTTGGGCCAGACAAGCCTGTCCAGGCCTTGGTGGGGGAGGACGCAGCATTCTCCTGTTTCCTGTCTCCTAAGACCAATGCAGAGGCCATGGAAGTGCGGTTCTTCAGGGGCCAGTTCTCTAGCGTGGTCCACCTCTACAGGGACGGGAAGGACCAGCCATTTATGCAGATGCCACAGTATCAAGGCAGGACAAAACTGGTGAAGGATTCTATTGCGGAGGGGCGCATCTCTCTGAGGCTGGAAAACATTACTGTGTTGGATGCTGGCCTCTATGGGTGCAGGATTAGTTCCCAGTCTTACTACCAGAAGGCCATCTGGGAGCTACAGGTGTCAGCACTGGGCTCAGTTCCTCTCATTTCCATCACGGGATATGTTGATAGAGACATCCAGCTACTCTGTCAGTCCTCGGGCTGGTTCCCCCGGCCCACAGCGAAGTGGAAAGGTCCACAAGGACAGGATTTGTCCACAGACTCCAGGACAAACAGAGACATGCATGGCCTGTTTGATGTGGAGATCTCTCTGACCGTCCAAGAGAACGCCGGGAGCATATCCTGTTCCATGCGGCATGCTCATCTGAGCCGAGAGGTGGAATCCAGGGTACAGATAGGAGATACCTTTTTCGAGCCTATATCGTGGCACCTGGCTACCAAAGTACTGGGAATACTCTGCTGTGGCCTATTTTTTGGCATTGTTGGACTGAAGATTTTCTTCTCCAAATTCCAGTGGAAAATCCAGGCGGAACTGGACTGGAGAAGAAAGCACGGACAGGCAGAATTGAGAGACGCCCGGAAACACGCAGTGGAGGTGACTCTGGATCCAGAGACGGCTCACCCGAAGCTCTGCGTTTCTGATCTGAAAACTGTAACCCATAGAAAAGCTCCCCAGGAGGTGCCTCACTCTGAGAAGAGATTTACAAGGAAGAGTGTGGTGGCTTCTCAGAGTTTCCAAGCAGGGAAACATTACTGGGAGGTGGACGGAGGACACAATAAAAGGTGGCGCGTGGGAGTGTGCCGGGATGATGTGGACAGGAGGAAGGAGTACGTGACTTTGTCTCCCGATCATGGGTACTGGGTCCTCAGACTGAATGGAGAACATTTGTATTTCACATTAAATCCCCGTTTTATCAGCGTCTTCCATAAATGACCAGTCCCTTATTTATACCCTGACATGTCGGTTTGAAGGCTTATTGAGGCCCTACATTGAGTATCCGTCCTATAATGAGCAAAATGGAACTCCCATAGTCATCTGCCCAGTCACCCAGGAATCAGAGAAAGAGGCCTCTTGGCAAAGGGCCTCTGCAATCCCAGAGACAAGCAACAGTGAGTCCTCCTCACAGGCATGGCCTGTCCACATGGGAGTCAGGTGTCATGGCTGCCCTGAGCTGGGAGGGAAGAAGGCTGACATTACATTTAGTTTGCTCTCACTCCATCTGGCTAAGTGATCTTGAAATACCACCTCTCAGGTGAAGAACCGTCAGGAATTCCCATCTCACAGGCTGTGGTGTAGATTAAGTAGACAAGGAATGTGAATAATGCTTAGATCTTATTGATGACAGAGTGTATCCTAATGGTTTGTTCATTATATTACACTTTCAGTAAAAAAAAAAAANOV20h, SNP13382441 ofSEQ ID NO: 504MW at 56747.1kDCG53449-03, Protein Sequence500 aaSNP Pos: 485SNP Change: Glu to GluMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRISLRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQWKIQAELDWRRKHGQAELRDARKHAVEVTLDPETAHPKLCVSDLKTVTHRKAPQEVPHSEKRFTRKSVVASQSFQAGKHYWEVDGGHNKRWRVGVCRDDVDRRKEYVTLSPDHGYWVLRLNGEHLYFTLNPRFISVFPRTPPTKIGVFLDYECGTISFFNINDQSLIYTLTCRFEGLLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSESSSQATTPFLPRGEM


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 20B.

TABLE 20BComparison of the NOV20 protein sequences.NOV20aMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFNOV20bMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFNOV20c--MNPVPQMEMQKSPMFCVAHSGSCRPELFLFGHLGSSPSLQRVLMIYLLYVFPQNOV20d--MNPVPQMEMQKSPMFCVAHSGSCRPELFLFGHLGSSPSLQRVLMIYLLYVFPQNOV20e-----------------QWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFNOV20fMALMLSLVLSLLKLGSGQWQVFGPDKPVQALVGEDAAFSCFLSPKTNAEAMEVRFFRGQFNOV20aSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRIS----------------------NOV20bSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRIS----------------------NOV20cGSGRCLGQTSLSRPWWGRTQHSPVSCLLKPM-----------------------------NOV20dGSGRCLGQTSLSRPWWGRTQHSPVSCLLKPMQRPWKCGSSGASSLAWSTSTGTGRTSHLCNOV20eSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRIS----------------------NOV20fSSVVHLYRDGKDQPFMQMPQYQGRTKLVKDSIAEGRIS----------------------NOV20a---------------------LRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVNOV20b---------------------LRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVNOV20c------------------------------------------------------------NOV20dRCHSIKAGQNCEGFYCGGAHLSRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVNOV20e---------------------LRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVNOV20f---------------------LRLENITVLDAGLYGCRISSQSYYQKAIWELQVSALGSVNOV20aPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVNOV20bPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVNOV20c------------------------------------------------------------NOV20dPLISITGYVDRDIQLLCQSSGWFPRPVQGAS------VVFVPCTLLCPPLNILFLFQGKSNOV20ePLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVNOV20fPLISITGYVDRDIQLLCQSSGWFPRPTAKWKGPQGQDLSTDSRTNRDMHGLFDVEISLTVNOV20aQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFNOV20bQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFNOV20c------------------------------------------------------------NOV20dRRNWVSMCHVLSLPHMVLPGPSLIHSLSLWTTLAAGWTGSTDWRRKHGQAELRDARKHAVNOV20eQENAGSISCSMRHAHLSREVESRVQIGDTFFEPI--------------------------NOV20fQENAGSISCSMRHAHLSREVESRVQIGDTFFEPISWHLATKVLGILCCGLFFGIVGLKIFNOV20aFSKFQCKRERE--------------------------------AWAGALFMVPAGTGSEMNOV20bFSKFQWKIQAELDWRRKHGQAELRDARKHAVEVTLDPETAHPKLCVSDLKTVTHRKAPQENOV20c------------------------------------------------------------NOV20dEVTLDPETAHP-------------------------------KLCVSDLKTVTHRKAPQENOV20e------------------------------------------------------------NOV20fFSKFQCKRERE--------------------------------AWAGALFMVPAGTGSEMNOV20aLPHP---AASLLLVLASRGPGPKKENPGGTGLEKKARTGRIERRPETRSGGDSGSRDGSPNOV20bVPHSEKRFTRKSVVASQSFQAGKHYWEVDGGHNKRWRVGVCRDDVDRRKEYVTLSPDHGYNOV20c------------------------------------------------------------NOV20dVPHSEKRFTRKSVVASQSFQAGKHYWEVDGGHNKRWRVGVCRDDVDRRKEYVTLSPDHGYNOV20e------------------------------------------------------------NOV20fLPHP---AASLLLVLASRGPGPKKENPGGTGLEKKARTGRIERRPETRSGGDSGSRDGSPNOV20aEALRF-------------------------------------------------------NOV20bWVLRLNGEHLYFTLNPRFISVFPRTPPTKIGVFLDYECGTISFFNINDQSLIYTLTCRFENOV20c------------------------------------------------------------NOV20dWVLRLNGEHLYFTLNPRFISVFPRTPPTKIGVFLDYECGTISFFNINDQSLIYTLTCRFENOV20e------------------------------------------------------------NOV20fEALRF-------------------------------------------------------NOV20a------------------------------------------------------------NOV20bGLLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSESSSQATTPFLPRNOV20c------------------------------------------------------------NOV20dGLLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSEVLLTGNHALPPQNOV20e------------------------------------------------------------NOV20f------------------------------------------------------------NOV20a---NOV20bGEMNOV20c---NOV20dG--NOV20e---NOV20f---NOV20a(SEQ ID NO: 490)NOV20b(SEQ ID NO: 492)NOV20c(SEQ ID NO: 494)NOV20d(SEQ ID NO: 496)NOV20e(SEQ ID NO: 498)NOV20f(SEQ ID NO: 500)


Further analysis of the NOV20a protein yielded the following properties shown in Table 20C.

TABLE 20CProtein Sequence Properties NOV20aSignalP analysis:Cleavage site between residues 18 and 19PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 12; peak value 10.45PSG score:6.05GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.59possible cleavage site: between 17 and 18>>> Seems to have a cleavable signal peptide (1 to 17)ALOM:Klein et al's method for TM region allocationInit position for calculation: 18Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 242-258−7.06PERIPHERALLikelihood = 2.17 (at 126)ALOM score: −7.06 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 8Charge difference: 0.0 C(1.0)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 259 to 347)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):1.22Hyd Moment(95):2.65G content:3D/E content:1S/T content:3Score: −6.60Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 11.8%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 293checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas' s algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: endoplasmic reticulum22.2%: Golgi22.2%: extracellular, including cell wall11.1%: plasma membrane>> prediction for CG53449-04 is end (k = 9)


A search of the NOV20a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 20D.

TABLE 20DGeneseq Results for NOV20aNOV20aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAU83681Human PRO protein, Seq ID No 180 -1 . . . 268264/268 (98%)e−152Homo sapiens, 500 aa.1 . . . 268265/268 (98%)[WO200208288-A2, 31-JAN-2002]ABB84917Human PRO1347 protein sequence1 . . . 268264/268 (98%)e−152SEQ ID NO: 202 - Homo sapiens, 5001 . . . 268265/268 (98%)aa. [WO200200690-A2, 03-JAN-2002]AAE20317Human B7-H13 protein #1 - Homo1 . . . 268264/268 (98%)e−152sapiens, 461 aa. [WO200202587-A1 ,1 . . . 268265/268 (98%)10-JAN-2002]ABB95523Human angiogenesis related protein1 . . . 268264/268 (98%)e−152PRO1347 SEQ ID NO: 202 - Homo1 . . . 268265/268 (98%)sapiens, 500 aa. [WO200208284-A2,31-JAN-2002]ABG95892Human secreted/transmembrane1 . . . 268264/268 (98%)e−152protein PRO1347 - Homo sapiens, 5001 . . . 268265/268 (98%)aa. [US2002119130-A1, 29-AUG-2002]


In a BLAST search of public sequence databases, the NOV20a protein was found to have homology to the proteins shown in the BLASTP data in Table 20E.

TABLE 20EPublic BLASTP Results for NOV20aNOV20aProteinResidues/Identities/AccessionMatchSimilarities for theExpectNumberProtein/Organism/LengthResiduesMatched PortionValueQ9H730Hypothetical protein FLJ21458 - 1 . . . 347347/347(100%)0.0Homo sapiens (Human), 347 aa. 1 . . . 347347/347(100%)Q9Y2C7Butyrophilin like receptor - Homo 1 . . . 157111/157(70%)5e−55sapiens (Human), 432 aa. 1 . . . 157126/157(79%)O73716B-G-like protein - Grus americana 7 . . . 268103/266(38%)3e−45(whooping crane), 280 aa.17 . . . 280154/266(57%)Q96DM5Hypothetical protein FLJ32535 - 2 . . . 252104/259(40%)3e−44Homo sapiens (Human), 315 aa.19 . . . 277159/259(61%)P18892Butyrophilin precursor (BT) - Bos 3 . . . 24996/254(37%)3e−42taurus (Bovine), 526 aa.13 . . . 266153/254(59%)


PFam analysis predicts that the NOV20a protein contains the domains shown in the Table 20F.

TABLE 20FDomain Analysis of NOV20aPfamNOV20aIdentities/ExpectDomainMatch RegionSimilaritiesValuefor theMatched Region


Example 21

The NOV21 clone was analyzedi and the nucleotide and encoded polypeptide sequences are shown in Table 21A.

TABLE 21ANOV21 Sequence AnalysisNOV21a, CG53908-01SEQ ID NO: 5052881 bpDNA SequenceORF Start: ATG at 87ORF Stop: TGA at 2784AGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGAATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21a, CG53908-01Protein SequenceSEQ ID NO: 506899 aaMW at 99063.0kDMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECNOV21b, CG53908-02SEQ ID NO: 507993 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCNOV21b, CG53908-02Protein SequenceSEQ ID NO: 508331 aaMW at 36604.8kDQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGNOV21c, 306075989SEQ ID NO: 5091009 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGGCTGCCCACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCTATTTGCGCAAGAACTTCGAGCAGGAGCCACTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCAGAAAACAGCCTGCGCCACCCTGTGCCCAGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCGAATTCGGCNOV21c, 306075989Protein SequenceSEQ ID NO: 510336 aaMW at 36996.3kDTGSQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLPNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQKTACATLCPVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGEFGNOV21d, CG53908-03SEQ ID NO: 5111009 bpDNA SequenceORF Start: at 1ORF Stop: at 1006CAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACNOV21d, CG53908-03Protein SequenceSEQ ID NO: 512335 aaMW at 36949.6kDQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNNOV21e, CG53908-04SEQ ID NO: 5132752 bpDNA SequenceORF Start: ATG at 46ORF Stop: TGA at 2740CCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGGCTGCCCACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCAGAAAACAGCCTGCGCCACCCTGTGCCCAGTAGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAGCAGACAAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCACTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGNOV21e, CG53908-04Protein SequenceSEQ ID NO: 514898 aaMW at 98841.9kDMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQKTACATLCPVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECNOV21f, CG53908-05SEQ ID NO: 5151002 bpDNA SequenceORF Start: at 7ORF Stop: at 997GGATCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGGCTGCCCACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCTATTTGCGCAAGAACTTCGAGCAGGAGCCACTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCAGAAAACAGCCTGCGCCACCCTGTGCCCAGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCTCGAGNOV21f, CG53908-05Protein SequenceSEQ ID NO: 516330 aaMW at 36417.7kDQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQKTACATLCPVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGNOV21g, CG53908-06SEQ ID NO: 5172575 bpDNA SequenceORF Start: at 3ORF Stop: TGA at 2502CAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGGCTGCCCACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCTATTTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACACTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCACCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGAATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGNOV21g, CG53908-06Protein SequenceSEQ ID NO: 518833 aaMW at 91922.0kDALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVTERSTDGSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHTASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECNOV21h, CG53908-07SEQ ID NO: 5192463 bpDNA SequenceORF Start: at 7ORF Stop: at 2458GGATCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGGCTGCCCACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCTATTTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGGCTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACACTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGAATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCAAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCGCTGAGTGCAAGCTTNOV21h, CG53908-07Protein SequenceSEQ ID NO: 520817 aaMW at 90314.2kDQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVDGSWSPWSKWSACGLGCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHTASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5212881 bpNOV21i, SNP13382444 ofORF Start: at 423ORF Stop: end of sequenceCG53908-01, DNA SequenceSNP Pos: 420SNP Change: C to TAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGTAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21i, SNP13382444 ofSEQ ID NO: 522MW at 98934.8kDCG53908-01, Protein SequenceSNP Pos: 112898 aaSNP Change: Gln to EndMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPEYNIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPELIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5232881 bpNOV21j, SNP13375491 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 1139SNP Change: A to GAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTACGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTGCACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21j, SNP13375491 ofSEQ ID NO: 524MW at 99063.0kDCG53908-01, Protein SequenceSNP Pos: 351899 aaSNP Change: Val to ValMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRISIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPELIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5252881 bpNOV21k, SNP13375492 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 1225SNP Change: T to CAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCCCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGGGGGCCCTTCCCCACACCGGGGAGANOV21k, SNP13375492 ofSEQ ID NO: 526MW at 99046.9kDCG53908-1, Protein SequenceSNP Pos: 380899 aaSNP Change: Leu to ProMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVPILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNTRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5272881 bpNOV21l, SNP13375493 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 1660SNP Change: C to TAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTGTATCAGCCTCCTCATCCCCCCAGATGTCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21l, SNP13375493 ofSEQ ID NO: 528MW at 99091.0kDCG53908-01, Protein SequenceSNP Pos: 525899 aaSNP Change: Ala to ValMARRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDVIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKTPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5292881 bpNOV21m, SNP13375135 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 1855SNP Change: G to AAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCACCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACTGGGGCCCTTCCCCACACCGGGGAGANOV21m, SNP13375135 ofSEQ ID NO:530MW at 99043.9kDCG53908-01, Protein SequenceSNP Pos: 590899 aaSNP Change: Arg to HisMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVPINSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLHLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5312881 bpNOV21n, SNP13375136 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 1965SNP Change: G to AAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACATCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21n, SNP13375136 ofSEQ ID NO: 532MW at 99077.0kDCG53908-01, Protein SequenceSNP Pos: 627899 aaSNP Change: Val to Ile+TL,1, MARVPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKYQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYIFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5332881 bpNOV21o, SNP13375137 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2025SNP Change: G to AAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCACCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGCGGAGANOV21o, SNP13375137 ofSEQ ID NO: 534MW at 99093.0kDCG53908-01, Protein SequenceSNP Pos: 647899 aaSNP Change: Ala to ThrMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGPLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAATKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5352881 bpNOV21p, SNP13375138 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2038SNP Change: A to GAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTACGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCCCGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAGGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21p, SNP13375138 ofSEQ ID NO: 536MW at 99091.0kDCG53908-01, Protein SequenceSNP Pos: 651899 aaSNP Change: Lys to ArgMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTPNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVIIAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLRLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5372881 bpNOV21q, SNP13375494 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2130SNP Change: G to CAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGCTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21q, SNP13375494 ofSEQ ID NO: 538MW at 99077.0kDCG53908-01, Protein SequenceSNP Pos: 682899 aaSNP Change: Val to LeuMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNTVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKELVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5392881 bpNOV21r, SNP13375495 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2305SNP Change: G to AAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGACACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21r, SNP13375495 ofSEQ ID NO: 540MW at 99121.0kDCG53908-01, Protein SequenceSNP Pos: 740899 aaSNP Change: Gly to AspMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARPRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNDTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5412881 bpNOV21s, SNP13375140 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2325SNP Change: T to CAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAc3TGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACCGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21s, SNP13375140 ofSEQ ID NO: 542MW at 99116.0kDCG53908-01, Protein SequenceSNP Pos: 747899 aaSNP Change: Cys to ArgMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSFAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHRTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5432881 bpNOV21t, SNP13375139 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2404Change: A to GGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTGGACCACGTGATCGAGCGCAGCACAGACGGGAGCAGTGGTGAGCCGACCATGGAGGTCCGCATTAATGTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCAGGCTTCCAGCCCGTCAGCATCAAGCCCAGCAAAGCAGACAACCCCCATCTGCTCACCATCCAGCCGGACCTCAGCACCACCACCACCTACCAGGGCAGTCTCTGTCCCCGGCAGGATGGGCCCAGCCCCAAGTTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGCACAAGCCGGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCCATCGTTAGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGGCGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCCTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGTGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21t, SNP13375139 ofSEQ ID NO: 544MW at 99005.0kDCG53908-01, Protein SequenceSNP Pos: 773899 aaSNP Change: Asp to GlyMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYTVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVIERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGGGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECSEQ ID NO: 5452881 bpNOV21u, SNP13375496 ofORF Start: ATG at 87ORF Stop: TGA at 2784CG53908-01, DNA SequenceSNP Pos: 2676SNP Change: T to CAGCTGGGGCTCCGGGCTGAGGCGCTAAAGCCGCCCTCCCGCCCGCGGGGCCCCGCGCCCGGCCCGCCCGCCTGCCCGCCCGCGGCCATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCGCCGCTTGGCTCCGCGGCTCGGGTGCCCAGCAGAGTGCCACCGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTTCCCCACTTCCTGGTGGAGCCCGAGGATGTGTACATCGTCAAGAACAAGCCAGTGCTGCTTGTGTGCAAGGCCGTGCCCGCCACGCAGATCTTCTTCAAGTGCAACGGGGAGTGGGTGCGCCAGGTTCTCAAGGCAGCAGGTCGAGAAGGTGTTCGGGCTGGAGGAATACTGGTGCCAGTGCGTGGCATGGAGCTCCTCGGGCACCACCAAGAGTCAGAAGGCCTACATCCGCATAGCCAGATTGCGCAAGAACTTCGAGCAGGAGCCGCTGGCCAAGGAGGTGTCCCTGGAGCAGGGCATCGTGCTGCCCTGCCGTCCACCGGAGGGCATCCCTCCAGCCGAGGTGGAGTGGCTCCGGAACGAGGACCTGGTGGACCCGTCCCTGGACCCCAATGTATACATCACGCGGGAGCACAGCCTGGTGGTGCGACAGGCCCGCCTTGCTGACACGGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCACGTCGCCGCAGCGCCTCCGCTGCTGTCATCGTCTACGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGCGCCAGCTGTGGGCGCGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCGCCTCTCAACGGGGGCGCTTTCTGTGAGGGGCAGAATGTCCATGACCGCACCGTCTCCTCTCTGCTTGTCTCTGTGGACGGCAGCTGGAGCCCGTGGAGCAAGTGGTCGGCCTGTGGGCTGGACTGCACCCACTGGCGGAGCCGTGAGTGCTCTGACCCAGCACCCCGCAACGGAGGGGAGGAGTGCCAGGGCACTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGTGTACACAGTGCTTCTGGCCCTGAGGACGTGGCCCTCTATGTGGGCCTCATCGCCGTGGCCGTCTGCCTGGTCCTGCTGCTGCTTGTCCTCATCCTCGTTTATTGCCGGAAGAAGGAGGGGCTGGACTCAGATGTGGCTGACTCGTCCATTCTCACCTCCAGCTCACCAATGGGCACCTGCTCAGCCCCCTGGGTGGCGGCCGCCACACACTGCACCACAGCTCTCCCACCTCTGAGGCCGAGGAGTTCGTCTCCCGCCTCTCCACCCAGAACTACTTCCGCTCCCTGCCCCGAGGCACCAGCAACATGACCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTAATACAGGTATCAGCCTCCTCATCCCCCCAGATGCCATACCCCGAGGGAAGATCTATGAGATCTACCTCACGCTGTGGACCCCCTGGCGTCCTGCTCACCCGGCCAGTCATCCTGGCTATGGACCACTGTGGGGAGCCCAGCCCTGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCGTGCGAGGGCAGCTGGGAGCAGGATGTGCTGCACCTGGGCGAGGAGGCGCCCTCCCACCTCTACTACTGCCAGCTGGAGGCCAGTGCCTGCTACGTCTTCACCGAGCAGCTGGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCGCCAAGCGCCTCAAGCTGCTTCTGTTTGCGCCGGTGGCCTGCACCTCCCTCGAGTACAACATCCGGGTCTACTGCCTGCATGACACCCACGATGCACTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGGGGACAGCTGATCCAGGAGCCACGGGTCCTGCACTTCAAGGACAGTTACCACAACCTGCGCCTATCCATCCACGATGTGCCCAGCTCCCTGTGGAAGAGTAAGCTCCTTGTCAGCTACCAGGAGATCCCCTTTTATCACATCTGGAATGGCACGCAGCGGTACTTGCACTGCACCTTCACCCTGGAGCGTGTCAGCCCCAGCACTAGTGACCTGGCCTGCAAGCTGTGGGTGTGGCAGGTGGAGGGCGACGGGCAGAGCTTCAGCATCAACTTCAACATCACCAAGGACACAAGGTTTGCTGAGCTGCTGGCTCTGGAGAGTGAAGCGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCAGAAGATAATTTCCAGCCTGGACCCACCCTGTAGGCGGGGTGCCGACTGGCGGACTCTGGCCCAGAAACTCCACCTGGACAGCCATCTCAGCTTCTTTGCCTCCAAGCCCAGCCCCACAGCCATGATCCTCAACCTGCGGGAGGCGCGGCACTTCCCCAACGGCAACCTCAGCCAGCTGGCTGCAGCAGTGGCTGGACTGGGCCAGCCAGACGCTGGCCTCTTCACAGTGTCGGAGGCTGAGTGCTGAGGCCGGCCAGGCCCGACACCTACACTCTCACCAGCTTTGGCACCCACCAAGGACAGGCAGAAGCCGGACAGGGGCCCTTCCCCACACCGGGGAGANOV21u, SNP13375496 ofSEQ ID NO: 546MW at 99032.9kDCG53908-01, Protein SequenceSNP Pos: 864899 aaSNP Change: Trp to ArgMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPATQIFFKCNGEWVRQVDHVTERSTDGSSGEPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKAYIRIARLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVHDRTVSSLLVSVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLLTIQPDLSTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQKLHLDSHLSFFASKPSPTAMILNLREARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAEC


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 21B.

TABLE 21 BComparison of the NOV21 protein sequences.NOV21aMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNQV21b-------------------------QQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNOV21c----------------------TGSQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNOV21d-------------------------QQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNOV21eMAVRPGLWPALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNOV21f-------------------------QQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNOV21g---------ALLGIVLAAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPNOV21h-------------------------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-TACATLCPVDGNOV21dVDG---------------------------------------------------------NOV21eVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQ-NVQKTACATLCPVDGNOV21fVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAFCEGQNVQK-TACATLCPVDGNOV21gVDG---------------------------------------------------------NOV21hVDG---------------------------------------------------------NOV21aSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTPNCTSDLCVHSASGPEDVNOV21bSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASG----NOV21cSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGEFG-NOV21dSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVNOV21eSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASGPEDVNOV21fSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHSASG----NOV21gSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHTASGPEDVNOV21hSWSPWSKWSACGLGCTHWRSRECSDPAPRNGGEECQGTDLDTRNCTSDLCVHTASGPEDVNOV21aALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21dALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSTLTSGFQPVSIKPSKADN---NOV21eALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLNOV21f------------------------------------------------------------NOV21gALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLNOV21hALYVGLIAVAVCLVLLLLVLILVYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLNOV21aLTIQPDLS-TTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVNOV21f------------------------------------------------------------NOV21gLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVNOV21hLTIQPDLSTTTTTYQGSLCPRQDGPSPKFQLTNGHLLSPLGGGRHTLHHSSPTSEAEEFVNOV21aSRLSTQNYFRSLPRGTSNNTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eSRLSTQNYFRSLPRGTSNNTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHNOV21f------------------------------------------------------------NOV21gSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHNOV21hSRLSTQNYFRSLPRGTSNMTYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHNOV21aKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eKFEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSNOV21f------------------------------------------------------------NOV21gKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSNOV21hKPEDVRLPLAGCQTLLSPIVSCGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSNOV21aWEQDVLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVANOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eWED-VLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVANOV21f------------------------------------------------------------NOV21gWED-VLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVANOV21hWED-VLHLGEEAPSHLYYCQLEASACYVFTEQLGRFALVGEALSVAAAKRLKLLLFAPVANOV21aCTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eCTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSNOV21f------------------------------------------------------------NOV21gCTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSNOV21hCTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLHFKDSYHNLRLSIHDVPSSNOV21aLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINOv21f------------------------------------------------------------NOV21gLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINOV21hLWKSKLLVSYQEIPFYHIWNGTQRYLHCTFTLERVSPSTSDLACKLWVWQVEGDGQSFSINOV21aNFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eNFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQNOV21f------------------------------------------------------------NOV21gNFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQNOV21hNFNITKDTRFAELLALESEAGVPALVGPSAFKIPFLIRQKIISSLDPPCRRGADWRTLAQNOV21aKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECNOV21b------------------------------------------------------------NOV21c------------------------------------------------------------NOV21d------------------------------------------------------------NOV21eKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECNOV21f------------------------------------------------------------NOV21gKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAvAGLGQPDAGLFTVSEAECNOV21hKLHLDSHLSFFASKPSPTAMILNLWEARHFPNGNLSQLAAAVAGLGQPDAGLFTVSEAECNOV21a(SEQ ID NO: 506)NOV21b(SEQ ID NO: 508)NOV21c(SEQ ID NO: 510)NOV21d(SEQ ID NO: 512)NOV21e(SEQ ID NO: 514)NOV21f(SEQ ID NO: 516)NOV21g(SEQ ID NO: 518)NOV21h(SEQ ID NO: 520)


Further analysis of the NOV21a protein yielded the following properties shown in Table 21C.

TABLE 21CProtein Sequence Properties NOV21aSignalP analysis:Cleavage site between residues 26 and 27PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 4; pos. chg 1; neg. chg 0H-region:length 16; peak value 9.57PSG score:5.17GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 1.79possible cleavage site: between 25 and 26>>> Seems to have a cleavable signal peptide (1 to 25)ALOM:Klein et al's method for TM region allocationInit position for calculation: 26Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 367-383−19.00PERIPHERALLikelihood = 0.85 (at 61)ALOM score: −19.00 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 12Charge difference: −1.0 C(1.0)-N(2.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 384 to 899)MITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):9.35Hyd Moment(95):6.17G content:5D/E content:1S/T content:3Score: −4.19Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 31 LRG|SGNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 9.2%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: AVRPnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 420LL at 455LL at 519LL at 554LL at 567LL at 652LL at 653LL at 725checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: vesicles of secretory system11.1%: extracellular, including cell wall>> prediction for CG53908-01 is end (k = 9)


A search of the NOV21 a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 21D.

TABLE 21D Geneseq Results for NOV21aNOV21aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAU79939Human UNC5-like protein NOV1 -1 . . . 899899/899(100%)0.0Homo sapiens, 899 aa.1 . . . 899899/899(100%)[WO200229038-A2, 11-APR-2002]AAU85403Human protein NOV1 - Homo sapiens,1 . . . 899888/900(98%)0.0898 aa. [WO200210216-A2, 07-FEB-1 . . . 898890/900(98%)2002]AAU97899Human netrin binding membrane1 . . . 899886/900(98%)0.0receptor UNC5H-1 protein - Homo1 . . . 898889/900(98%)sapiens, 898 aa. [WO200233080-A2,25-APR-2002]AAU10543Rat netrin receptor UNC5H1 (YSG7)1 . . . 899852/900(94%)0.0polypeptide - Rattus sp, 898 aa.1 . . . 898871/900(96%)[WO200175440-A2, 11-OCT-2001]AAU97900Rat netrin binding membrane receptor1 . . . 899852/900(94%)0.0UNC5H-1 protein - Rattus sp, 898 aa.1 . . . 898871/900(96%)[WO200233080-A2, 25-APR-2002]


In a BLAST search of public sequence databases, the NOV21a protein was found to have homology to the proteins shown in the BLASTP data in Table 21E.

TABLE 21EPublic BLASTP Results for NOV21aNOV21aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8K1S4Netrin receptor Unc5h1 - Mus 1 . . . 899858/900 (95%)0.0musculus (Mouse), 898 aa. 1 . . . 898879/900 (97%)O08721Transmembrane receptor UNC5H1 - 1 . . . 899852/900 (94%)0.0Rattus norvegicus (Rat), 898 aa. 1 . . . 898871/900 (96%)Q96GP4Similar to transmembrane receptor359 . . . 899540/542 (99%)0.0Unc5H1 - Homo sapiens (Human), 4 . . . 544540/542 (99%)544 aa (fragment).O08747Rostral cerebellar malformation 9 . . . 898515/913 (56%)0.0protein - Mus musculus (Mouse), 931 26 . . . 930668/913 (72%)aa.Q8CD16Unc5 homolog - Mus musculus 9 . . . 898515/932 (55%)0.0(Mouse), 950 aa. 26 . . . 949668/932 (71%)


PFam analysis predicts that the NOV21a protein contains the domains shown in the Table 21F.

TABLE 21FDomain Analysis of NOV21aIdentities/SimilaritiesPfamNOV21afor theExpectDomainMatch RegionMatched RegionValueig163 . . . 22317/63(27%)0.001538/63(60%)tsp_1246 . . . 28720/53(38%)0.0003630/53(57%)tsp_1303 . . . 35021/53(40%)0.001732/53(60%)ZU5495 . . . 59847/115(41%)6.3e−56103/115(90%)death818 . . . 89826/88(30%)3.5e−1464/88(73%)


Example 22.

The NOV22 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 22A.

TABLE 22ANOV22 Sequence AnalysisNOV22a, CG53944-02SEQ ID NO: 5471887 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 1885ATGGCCATCCTCCCGTTGCTCCTGTGCCTGCTGCCGCTGGCCCCTGCCTCATCCCCACCCCAGTCAGCCACACCCAGCCCATGTCCCCGCCGCTGCCGCTGCCAGACACAGTCGCTGCCCCTAAGCGTGCTGTGCCCAGGGGCAGGCCTCCTGTTCGTGCCACCCTCGCTGGACCGCCGGGCAGCCGAGCTGCGGCTGGCAGACAACTTCATCGCCTCCGTGCGCCGCCGCGACCTGGCCAACATGACAGGCCTGCTGCATCTGAGCCTGTCGCGGAACACCATCCGCCACGTGGCTGCCGGCGCCTTCGCCGACCTGCGGGCCCTGCGTGCCCTGCACCATCCTCAGCAACAACCAGCTGGCAGCGCTGGCGGCCGGCGCCCTGGATGATTGTGCCGAGACACTGGAGGACCTCGACCTCTCCTACAACAACCTCGAGCAGCTGCCCTGGGAGGCCCTGGGCCGCCTGGGCAACGTCAACACGTTGGGCCTCGACCACAACCTGCTGGCTTCTGTGCCCGCCGGCGCTTTTTCCCGCCTGCACAAGCTGGCCCGGCTGGACATGACCTCCAACCGCCTGACCACAATCCCACCCGACCCACTCTTCTCCCGCCTGCCCCTGCTCGCCAGGCCCCGGGGCTCGCCCGCCTCTGCCCTGGTGCTGGCCTTTGGCGGGAACCCCCTGCACTGCAACTGCGAGCTGGTGTGGCTGCGTCGCCTGGCGCGGGAGGACGACCTCGAGGCCTGCGCGTCCCCACCTGCTCTGGGCGGCCGCTACTTCTGGGCGGTGGGCGAGGAGGAGTTTGTCTGCGAGCCGCCCGTGGTGACTCACCGCTCACCACCTCTGGCTGTGCCCGCAGGTCGGCCGGCTGCCCTGCGCTGCCGGGCAGTGGGGGACCCAGAGCCCCGTGTGCGTTGGGTGTCACCCCAGGGCCGGCTGCTAGGCAACTCAAGCCGTGCCCGCGCCTTCCCCAATGGGACGCTGGAGCTGCTGGTCACCGAGCCGGGTGATGGTGGCATCTTCACCTGCATTGCGGCCAATGCAGCTGGCGAGGCCACAGCTGCTGTGGAGCTGACTGTGGGTCCCCCACCACCTCCTCAGCTAGCCAACAGCACCAGCTGTGACCCCCCGCGGGACGGGGATCCTGATGCTCTCACCCCACCCTCCGCTGCCTCTGCTTCTGCCAAGGTGGCCGACACTGGGCCCCCTACCGACCGTGGCGTCCAGGTGACTGAGCACGCGGCCACAGCTGCTCTTGTCCAGTGGCCGGATCAGCGGCCTATCCCGGGCAGAGAGCCGCTCGTTCCTGCTGACGGACCTGGCGTCAGGCCGGACCTACGATCTGTGCGTGCTCGCCGTGTATGAGGACAGCGCCACGGGGCTCACGGCCACGCGGCCTGTGGGCTGCGCCCGCTTCTCCACCGAACCTGCGCTGCGGCCATGCGGGGCGCCGCACGCTCCCTTCCTGGGCGGCACGATGATCATCGCGCTGGGCGGCGTCATCGTAGCCTCGGTACTGGTCTTCATCTTCGTGCTGCTAATGCGCTACAAGGTGCACGGCGGCCAGCCCCCCGGCAAGGCCAAGATTCCCGCGCCTGTTAGCAGCGTTTGCTCCCAGACCAACGGCGCCCTGGGCCCCACGCCCACGCCCGCCCCGCCCGCCCCGGAGCCCGCGGCGCTCAGGGCCCACACCGTGGTCCAGCTGGACTGCGAGCCCTGGGGGCCCGGCCACGAACCTGTGGGACCCTAGNOV22a, CG53944-02Protein SequenceSEQ ID NO: 5481628 aaMW at 66259.1kDMAILPLLLCLLPLAPASSPPQSATPSPCPPRCRCQTQSLPLSVLCPGAGLLFVPPSLDRRAAELRLADNFIASVRRRDLANMTGLLHLSLSRNTIRHVAAGAFADLPALRALHLDGNRLTSLGEGQLRGLVNLRHLILSNNQLAALAAGALDDCAETLEDLDLSYNNLEQLPWEALGRLGNVNTLGLDHNLLASVPAGAFSRLHKLARLDMTSNRLTTIPPDPLFSRLPLLARPRGSPASALVLAFGGNPLHCNCELVWLRRLAREDDLEACASPPALGGRYFWAVGEEEFVCEPPVVTHRSPPLAVPAGRPAALRCRAVGDPEPRVRWVSPQGRLLGNSSRAPAFPNGTLELLVTEPGDGGIFTCIAANAAGEATAAVELTVGPPPPPQLANSTSCDPPRDGDPDALTPPSAASASAKVADTGPPTDRGVQVTEHGATAALVQWPDQRPIPGIRMYQIQYNSSADDILVYRMIPAESRSFLLTDLASGRTYDLCVLAVYEDSATGLTATRPVGCARFSTEPALRPCGAPHAPFLGGTMIIALGGVIVASVLVFIFVLLMRYKVHGGQPPGKAKIPAPVSSVCSQTNGALGPTPTPAPPAPEPAALRAHTVVQLDCEPWGPGHEPVGPNOV22b, CG53944-01SEQ ID NO: 5491887 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 1885ATGGCCATCCTCCCGTTGCTCCTGTGCCTGCTGCCGCTGGCCCCTGCCTCATCCCCACCCCAGTCAGCCACACCCAGCCCATGTCCCCGCCGCTGCCGCTGCCAGACACAGTCGCTGCCCCTAAGCGTGCTGTGCCAACTTCATCGCCTCCGTGCGCCGCCGCGACCTGGCCAACATGACAGGCCTGCTGCATCTGAGCCTGTCGCGGAACACCATCCGCCACGTGGCTGCCGGCGCCTTCGCCGACCTGCGGGCCCTGCGTGCCCTGCACCTGGATGGCAACCGGCTGACCTCACTGGGCGAGGGCCAGCTGCGCGGCCTGGTCAACTTGCGCCACCTCATCCTCAGCAACAACCAGCTGGCAGCGCTGGCGGCCGGCGCCCTGGATGATTGTGCCGAGACACTGGAGGACCTCGACCTCTCCTACAACAACCTCGAGCAGCTGCCCTGGGAGGCCCTGGGCCGCCTGGGCAACGTCAACACGTTGGGCCTCGACCACAACCTGCTGGCTTCTGTGCCCGCCGGCGCTTTTTCCCGCCTGCACAAGCTGGCCCGGCTGGACATGACCTCCAACCGCCTGACCACAATCCCACCCGACCCACTCTTCTCCCGCCTGCCCCTGCTCGCCAGGCCCCGGGGCTCGCCCGCCTCTGCCCTGGTGCTGGCCTTTGGCGGGAACCCCCTGCACTGCAACTGCGAGCTGGTGTGGCTGCGTCGCCTGGCGCGGGAGGACGACCTCGAGGCCTGCGCGTCCCCACCTGCTCTGGGCGGCCGCTACTTCTGGGCGGTGGGCGAGGAGGAGTTTGTCTGCGAGCCGCCCGTGGTGACTCACCGCTCACCACCTCTGGCTGTGCCCGCAGGTCGGCCGGCTGCCCTGCGCTGCCGGGCAGTGGGGGACCCAGAGCCCCGTGTGCGTTGGGTGTCACCCCAGGGCCGGCTGCTAGGCAACTCAAGCCGTGCCCGCGCCTTCCCCAATGGGACGCTGGAGCTGCTGGTCACCGAGCCGGGTGATGGTGGCATCTTCACCTGCATTGCGGCCAATGCAGCTGGCGAGGCCACAGCTGCTGTGGAGCTGACTGTGGGTCCCCCACCACCTCCTCAGCTAGCCAACAGCACCAGCTGTGACCCCCCGCGGGACGGGGATCCTGATGCTCTCACCCCACCCTCCGCTGCCTCTGCTTCTGCCAAGGTGGCCGACACTGGGCCCCCTACCGACCGTGGCGTCCAGGTGACTGAGCACGGGGCCACAGCTGCTCTTGTCCAGTGGCCGGATCAGCGGCCTATCCCGGGCATCCGCATGTACCAGATCCAGTACAACAGCTCGGCTGATGACATCCTCGTCTACAGGATGATCCCGGCGGAGAGCCGCTCGTTCCTGCTGACGGACCTGGCGTCAGGCCGGACCTACGATCTGTGCGTGCTCGCCGTGTATGAGGACAGCGCCACGGGGCTCACGGCCACGCGGCCTGTGGGCTGCGCCCGCTTCTCCACCGAACCTGCGCTGCGGCCATGCGGGGCGCCGCACGCTCCCTTCCTGGGCGGCACGATGATCATCGCGCTGGGCGGCGTCATCGTAGCCTCGGTACTGGTCTTCATCTTCGTGCTGCTAATGCGCTACAAGGTGCACGGCGGCCAGCCCCCCGGCAAGGCCAAGATTCCCGCGCCTGTTAGCAGCGTTTGCTCCCAGACCAACGGCGCCCTGGGCCCCACGCCCACGCCCGCCCCGCCCGCCCCGGAGCCCGCGGCGCTCAGGGCCCACACCGTGGTCCAGCTGGACTGCGAGCCCTGGGGGCCCGGCCACGAACCTGTGGGACCCTAGNOV22b, CG53944-01Protein SequenceSEQ ID NO: 550628 aaMW at 66259.1kDMAILPLLLCLLPLAPASSPPQSATPSPCPRRCRCQTQSLPLSVLCPGAGLLFVPPSLDRRAAELRLADNFIASVRRRDLANMTGLLHLSLSRNTIRHVAAGAFADLRALRALHLDGNRLTSLGEGQLRGLVNLRHLILSNNQLAALAAGALDDCAETLEDLDLSYNNLEQLPWEALGRLGNVNTLGLDHNLLASVPAGAFSRLHKLARLDMTSNRLTTIPPDPLFSRLPLLARPRGSPASALVLAFGGNPLHCNCELVWLRRLAREDDLEACASPPALGGRYFWAVGEEEFVCEPPVVTHRSPPLAVPAGRPAALRCRAVGDPEPRVRWVSPQGRLLGNSSRAPAFPNGTLELLVTEPGDGGIFTCIAANAAGEATAAVELTVGPPPPPQLANSTSCDPPRDGDPDALTPPSAASASAKVADTGPPTDRGVQVTEHGATAAIJVQWPDQRPIPGIRMYQIQYNSSADDILVYRMPAESRSFLLTDLASGRTYDLCVLAVYEDSATGLTATRPVGCARFSTEPALRPCGAPHAPFLGGTMIIALGGVIVASVLVFIFVLLMRYKVHGGQPPGKAKIPAPVSSVCSQTNGALGPTPTPAPPAPEPAALRAHTVVQLDCEPWGPGHEPVGP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 22B.

TABLE 22BComparison of the NOV22 protein sequences.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(SEQ ID NO: 548)NOV22b(SEQ ID NO: 550)


Further analysis of the NOV22a protein yielded the following properties shown in Table 22C.

TABLE 22CProtein Sequence Properties NOV22aSignalP analysis:Cleavage site between residues 17 and 18PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 29; peak value 9.99PSG score:5.59GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 4.65possible cleavage site: between 16 and 17>>> Seems to have a cleavable signal peptide (1 to 16)ALOM:Klein et al's method for TM region allocationInit position for calculation: 17Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 543-559−12.79PERIPHERALLikelihood = 2.44 (at 363)ALOM score: −12.79 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 8Charge difference: 2.0 C(3.0)-N(1.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptide>>> membrane topology: type 1a (cytoplasmic tail 560 to 628)MITDISC:discrimination of mitochondrial targeting seqR content:3Hyd Moment (75):2.94Hyd Moment (95):1.64G content:2D/E content:1S/T content:9Score: −1.46Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 43 CRC|QTNUCDISC: discrimination of nuclear localization signalspat 4: nonepat7: PCPRRCR (3) at 27pat7: PRRCRCQ (5) at 29bipartite: nonecontent of basic residues: 8.4%NLS Score: 0.22KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: extracellular, including cell wall22.2%: Golgi22.2%: endoplasmic reticulum11.1%: plasma membrane>> prediction for CG53944-02 is exc (k = 9)


A search of the NOV22a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 22D.

TABLE 22DGeneseq Results for NOV22aNOV22aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABP69326Human polypeplide SEQ ID NO 1373 -1 . . . 628628/628(100%)0.0Homo sapiens, 628 aa.1 . . . 628628/628(100%)[WO200270539-A2, 12-SEP-2002]AAG67523Amino acid sequence of a human1 . . . 628628/628(100%)0.0secreted polypeptide - Homo sapiens,1 . . . 628628/628(100%)628 aa. [WO200166690-A2, 13-SEP-2001]AAB84469Amino acid sequence of an interferon1 . . . 628628/628(100%)0.0omega-1 like protein NOV2 - Homo1 . . . 628628/628(100%)sapiens, 628 aa. [WO200142471-A2,14-JUN-2001]AAG65805Human leucine-rich repeat (LRR) family1 . . . 628626/628(99%)0.0member, 33395 polypeptide - Homo1 . . . 628627/628(99%)sapiens, 628 aa. [WO200172827-A2,04-OCT-2001]ABG34079Human Pro peptide #51 - Homo1 . . . 628627/628(99%)0.0sapiens, 627 aa. [WO200224888-A2,1 . . . 627627/628(99%)28-MAR-2002]


In a BLAST search of public sequence databases, the NOV22a protein was found to have homology to the proteins shown in the BLASTP data in Table 22E.

TABLE 22EPublic BLASTP Results for NOV22aNOV22aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9BTNOHypothetical protein - Homo sapiens1 . . . 628628/628(100%)0.0(Human), 628 aa.1 . . . 628628/628(100%)CAD10239Sequence 1 from Patent WO0172827 -1 . . . 628626/628(99%)0.0Homo sapiens (Human), 628 aa.1 . . . 628627/628(99%)Q8BLY3Hypothetical leucine-rich repeat1 . . . 628603/628(96%)0.0containing protein - Mus musculus1 . . . 626611/628(97%)(Mouse), 626 aa.Q9ULH4Hypothetical protein KIAA1246 -23 . . . 603 310/585(52%)e−174Homo sapiens (Human), 832 aa59 . . . 639 398/585(67%)(fragment).Q9BE71Hypothetical 84.7 kDa protein -23 . . . 603 309/585(52%)e−174Macaca fascicularis (Crab eating16 . . . 596 397/585(67%)macaque) (Cynomolgus monkey),789 aa.


PFam analysis predicts that the NOV22a protein contains the domains shown in the Table 22F.

TABLE 22FDomain Analysis of NOV22aIdentities/SimilaritiesPfamNOV22a Matchfor the MatchedDomainRegionRegionExpect ValueLRR 84 . . . 107 8/25 (32%)0.8621/25 (84%)LRR132 . . . 15510/25 (40%)0.2720/25 (80%)LRR157 . . . 18012/25 (48%)0.02619/25 (76%)LRRCT249 . . . 29421/55 (38%)4.3e−0537/55 (67%)ig310 . . . 36815/62 (24%)1.2e−0544/62 (71%)fn3425 . . . 50518/84 (21%)7.1e−0555/84 (65%)


Example 23.

The NOV23 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 23A.

TABLE 23ANOV23 Sequence AnalysisNOV23a, CG54308-04SEQ ID NO: 5511284 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 1276ATGGACTCTCTTGTTACAGCAAACACCAAATTTTGCTTTGATCTTTTTCAAGAGATAGGCAAAGATGATCGTCATAAAAACATATTTTTCTCTCCCCTGAGCCTCTCAGCTGCCCTTGGTATGGTACGCTTGGGTGCTAGAAGTGACAGTGCACATCAGATTGATGAGGTACTACACTTCGACGAATTTTCCCAGAATGAAAGCAAAGAACCTGACCCTTGTCTGAAAAGCAACAAACAAAAGTGCTGGCTGACAGCTCTCTGGAGGGGGCAGAAAAAAACGACAGAGCCTCTGGATCAGCAGGCTGGGTCCTTAAACAATGAGAGCGGACTGGTCAGCTGCTACTTTGGGCAGCTTCTCTCCAAATTAGACAGGATCAAGACTGATTACACACTGAGTATTGCCAACAGGCTTTATGGAGAGCAGGAATTCCCAATCTGTCAGGAATACTTAGATGGTGTGATTCAATTTTACCACACGACGATTGAAAGTGTTGATTTCCAAAAAAACCCTGAAAAATCCAGACAAGAGATTAACTTCTGGGTTGAATGTCAATCCCAAGGTAAAATCAAGGAACTCTTCAGCAAGGACGCTATTAATGCTGAGACTGTGCTGGTACTGGTGAATGCTGTTTACTTCAAGGCCAAATGGGAAACATACTTTGACCATGAAAACACGGTGGATGCACCTTTCTGTCTAAATGCGAATGAAAACAAGAGTGTGAAGATGATGATGCAAAAAGGCCTCTACAGAATTGGCTTCATAGAGGAGGTGAAGGCACAGATCCTGGAAATGAGGTACACCAAGGGGAAGCTCAGCATGTTCGTGCTGCTGCCATCTCACTCTAAAGATAACCTGAAGGGTCTGGAAGAGCTTGAAAGGAAAATCACCTATGAAAAAATGGTGGCTGGAGCAGCTCAGAAAACATGTCAGAAGAAATCGGTGGTCCTGTCCTTCCCCCGGTTCACCCTGGAAGACAGCTATGATCTCAATTCCATTTTACAAGACATGGGCATTACGGATATCTTTGATGAAACGAGGGCTGATCTTACTGGAATCTCTCCAAGTCCCAATTTGTACTTGTCAAAAATTATCCACAAAACCTTTGTGGAGGTGGATGAAAACGGTACCCAGGCAGCTGCAGCCACTGGGGCTGTTGTCTCGGAAAGGTCACTACGATCTTGGGTGGAGTTTAATGCCAACCACCCTTTTCTCTTTTTCATTAGACACAACAAAACCCAAACCATTCTCTTTTATGGAAGGGTCTGCTCTCCTTAAAAGGGGNOV23a, CG54308-04Protein SequenceSEQ ID NO: 552425 aaMW at 48476.3kDMDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHFDEFSQNESKEPDPCLKSNKQKVLADSSLEGQKKTTEPLDQQAGSLNNESGLVSCYFGQLLSJHDRIKTDYTLSIANRLYGEQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINFWVECQSQGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNANENKSVKMMMQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYEKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYGRVCSPNOV23b, CG54308-01SEQ ID NO: 5531200 bpDNA SequenceORF Start: ATG at 7ORF Stop: TAA at 1192TTTACAATGGACTCTCTTGTTACAGCAAACACCAAATTTTGCTTTGATCTTTTTCAAGAGATAGGCAAAGATGATCGTCATAAAAACATATTTTTCTCTCCCCTGAGCCTCTCAGCTGCCCTTGGTATGGTACGCTGAAAGCAAAGAACCTGCTGGGTCCTTAAACAATGAGAGCGGACTGGTCAGCTGCTACTTTGGGCAGCTTCTCTCCAAATTAGACAGGATCAAGACTGATTACACACTGAGTATTGCCAACAGGCTTTATGGAGAGCAGGAATTCCCAATCTGTCAGGAATACTTAGATGGTGTGATTCAATTTTACCACACGACGATTGAAAGTGTTGATTTCCAAAAAAACCCTGAAAAATCCAGACAAGAGATTAACTTCTGGGTTGAATGTCAATCCCAAGGTAAATCAAGGACCTCTTCAGCAAGGACGCTATTAATGCTGAGACTGTGCTGGTACTGGGTGAATGCTGTTTACTTCAAGGCCAAATGGGAAACATACTTTGACCATGAAAACACGGTGGATGCACCTTTCTGTCTAAATCAGAATGAAAACAAGAGTGTGAAGATGATGACGCAAAAAGGCCTCTACAGAATTGGCTTCATAGAGGAGGTGAAGGCACAGATCCTGGAAATGAGGTACACCAAGGGGAAGCTCAGCATGTTCGTGCTGCATGGTGGCCTGGAGCAGCTCAGAAAACATGTCAGAAGAATCGGTGGTCCTGTCCTTCCCCCGGTTCACCCTGGAAGACAGCTATGATCTCAATTCCATTTTACAAGACATGGGCATTACGGATATCTTTGATGAAACGAGGGCTGATCTTACTGGAATCTCTCCAAGTCCCAATTTGTACTTGTCAAAAATTATCCACAAAACCTTTGTGGAGGTGGATGAAAACGGTACCCAGGCAGCTGCAGCCACTGGGGCTGTTGTCTCGGAAAGGTCAAACCATTCTCTTTTATGGCAGGGTCTGCTCTCCTTAAAAGGGGNOV23b, CG54308-01Protein SequenceSEQ ID NO: 554395 aaMW at 45176.6kDMDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHFNEFSQNESKEPAGSLNNESGLVSCYFGQLLSKLDRIKTDYTLSIANRLYGEQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINFWVECQSQGKIKDLFSKDAINAETVLVLVNAVYFKAKEWTYFDHENTVDAPFCLNQNENKSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYEKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYGRVCSPNOV23c, CG54308-02SEQ ID NO: 5551278 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceACCATGGACTCTCTTGTTACAGCAAACACCAAATTTTGCTTTGATCTTTTTCAAGAGATAGGCAAAGATGATCGTCATAAAAACATATTTTTCTCTCCCCTGAGCCTCTCAGCTGCCCTTGGTATGGTACGCTTGGGTGCTAGAAGTGACAGTGCACATCAGATTGATGAGGTACTACACTTCAACGAATTTTCCCAGAATGAAAGCAAAGAACCTGACCCTTGTCTGAAAGCAACAAACAAAAAGTGCTGGACTGACAGCTCTCTGGAGGGGCAGAAAAAAACGACAGAGCCTCTGGATCAGCAGGCTGGGTCCTTAAACAATGAGAGcGGACTGGTCAGCTGCTACTTTGGGCAGCTTCTCTCCAAATTAGACAGGATCAAGACTGATTACACACTGAGTATTGCCAACAGGCTTTATGGAGGGCAGGAATTCCCAATCTGTCAGGAATACTTAGATGGTGTGATTCAATTTTACCACACGACGATTGAAAGTGTTGATTTCCAAAAAAACCCTGAAAAATCCAGACAAGAGATTAACTTCTGGGTTGAATGTCAATCCCAAGGTAAAATCAAGGAACTCTTCAGCAAGGACGCTATTAATGCTGAGACTGTGCTGGTACTGGTGAATGCTGTTTACTTCAAGGCCAAATGGGAAACATACTTTGACCATGAAAACACGGTGGATGCACCTTTCTGTCTAAATGCGAATGAAAACAAGAGTGTGAAGATGATGACGCAAAAAGGCCTCTACAGAATTGGCTTCATAGAGGAGGTGAAGGCACAGATCCTGGAAATGAGGTACACCAAGGGGAAGCTCAGCATGTTCGTGCTGCTGCCATCTCACTCTAAAGATAACCTGAAGGGTCTGGAAGAGCTTGAAAGGAAAATCACCTATGAAAAAATGGTGGCCTGGAGCAGCTCAGAAAACATGTCAGAAGAATCGGTGGTCCTGTCCTTCCCCCGGTTCACCCTGGAAGACAGCTATGATCTCAATTCCATTTTACAAGACATGGGCATTACGGATATCTTTGATGAAACGAGGGCTGATCTTACTGGAATCTCTCCAAGTCCCAATTTGTACTTGTCAAAAATTATCCACAAAACCTTTGTGGAGGTGGATGAAAACGGTACCCAGGCAGCTGCAGCCACTGGGGCTGTTGTCTCGGAAAGGTCACTACGATCTTGGGTGGAGTTTAATGCCAACCACCCTTTTCTCTTTTTCATTAGACACAACAAAACCCAAACCATTCTCTTTTATGGCAGGGTCTGCTCTCCTNOV23c, CG54308-02Protein SequenceSEQ ID NO: 556426 aaMW at 48474.3kDTMDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHFNEFSQNESKEPDPCLKSNKQKVLADSSLEGQKKTTEPLDQQAGSLNNESGLVSCYFGQLLSKLDRIKTDYTLSIANRLYGGQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINFWVECQSQGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNANENKSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYEKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYGRVCSPNOV23d, CG54308-03SEQ ID NO: 5571285 bpDNA SequenceORF Start: ATG at 8ORF Stop: TAA at 1277TTTTACAATGGACTCTCTTGTTACAGCAAACACCAAATTTTGCTTTGATCTTTTTCAAGAGATAGGCAAAGATGATCGTCATAAAAACATATTTTTCTCTCCCCTGAGCCTCTCAGCTGCCCTTGGTATGGTACGCTTGGGTGCTATAAGTGACAGTGCACATCACATTGATGAGGTACGTGTCCACTATTCCCAGAATGAAAGCAAAGAACCTGACCCTTGTCTGAAAAGCAACAAACAAAAAGTGCTGGCTGACAGCTCTCTGGAGGGGCAGAAAAAAACGACAGAGCCTCTGGATCAGCAGGCTGGGTCCTTAAACAATGGGAGCGGACTGGTCAGCCAGGCTTTATGGAGAGCGGGAATTCCCAATCTGTCAGGAATACTTAGATGGTGTGATTCAATTTTACCACACGACGATTGAAAGTGTTGATTTCCAAAAAAACCCTGAAAAATCCAGACAAGAGATTAACTTCTGGGTTGAATGTCAATCCCAAGGTAAAATCAAGGAACTCTTCAGCAAGGACGCTATTAATGCTGAGACTGTGCTGGTACTGGTGAATGCTGTTTACTTCAAGGCCAAATGGGAAAACATACTTTGACCATGAAACACGGTGGATGCACCTTTCTGTCTAAATGCGAATGAAAACAAGAGTGTGAAGATGATGACGCAAAAAGGCCTCCAGCATGTTCGTGCTGCTGCCATCTCACTCTAAAGATAACCTGAAGGGTCTGGAAGAGCTTGAAAGGAAAATCACCTATGAAAAAATGGTGGCCTGGAGCAGCTCAGAAAACATGTCAGAAGAATCGGTGGTCCTGTCCTTCCCCCGGTTCACCCTGGAAGACAGCTATGATCTCAATTCCATTTTACAAGACATGGGCATTACGGATATCTTTGATGAAACGAGGGCTGATCTTACTGGAATCTCTCCAAGTCCCAATTTGTACTTGTCAAAAATTATCCACAAAACCTTTGTGGAGGTGGATGAAAACGGTACCCAGGCAGCTGCAGCCACTGGGGCTGTTGTCTCGGAAAGGTCACTACGATCTTGGGTGGAGTTTAATGCCAACCACCCTTTTCTCTTTTTCATTAGACACAACAAAACCCAAACCATTCTCTTTTATGGAAGGGTCTGCTCTCCTTAAAAGGGGNOV23d, CG54308-03Protein SequenceSEQ ID NO: 558423 aaMW at 48136.0kDMDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGAISDSAHHIDEVRVHYSQNESKEPDPCLKSNKQKVLADSSLEGQKKTTEPLDQQAGSLNNGSGLVSCYFGQLLSKLDRIKTDYTLSIADRLLVNAVYFKAXWETYFDHENTVDAPFCLNANENKSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYEKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYGRVCSPNOV23e, CG54308-05SEQ ID NO: 5591196 bpDNA SequenceORF Start: ATG at 60ORF Stop: TAA at 1155CCAATCACCATTTTCTCTTTCTCCTTTTTTTTTGGTTTTAGATCGTTATAAGTTTTACAATGGACTCTCTTGTTACAGCAAACACCAAATTTTGCTTTGATCTTTTTCAAGAGATAGGCAAAGATGATCGTCATAAAAACATATTTTTCTCTCCCCTGAGCCTCTCAGCTGCCCTTGGTATGGTACGCTTGGGTGCTAGAAGTGACAGTGCACATCAGATTGATGAGGTACGTTCCTTAAACAATGAGAGCGGACTGGTCAGCTGCTACTTTGGGCAGCTTCTCTCCAAATTAGACAGGATCAAGACTGATTACACACTGAGTATTGCCAACAGGCTTTATGGAGAGTCCAGCCTGGGAGACAAGAGCGAAACTCTGTCTCAAAAAAAAAAAAAAAAAATTATCTACACAAATGCTTTTGATACAATTCATACTCAGGATATTCTCTGGGATCTTTTTTTTAGGTAAATCAAGGAACTCTTCAGCAAGGACGCTATTAATGCTGAGACTGTGCTGGTACTGGTGATTGCTGTTTACTTCAAGGCCAAATGGGAACATACTTTGACCATGAAAACACGGTGGATGCACCTTTCTGTCTLAATCAGAATGAAAAACAAGAGTGTGAAGATGATGACGCAAAAAGGCCTCTACAGAATTGGCTTCATAGAGGAGGTGAAGGCACAGATCCTGGAAATGAGGTACACCAAGGGGAAGCTCAGCATGTTCGTGCTGCTGCCATCTCACTCTAAAGATAACCTGAAGGGTATCACCTATGAAAAAATGGTGGCCTGGAGCAGCTCAGAAAACATGTCAGAAGAATCGGTGGTCCTGTCCTTCCCCCGGTTCACCCTGGAAGACAGCTATGATCTCAATTCCATTTTACAAGACATGGGCATTACGGATATCTTTGATGAAACGAGGGCTGATCTTACTGGAATCTCTCCAAGTCCCAATTTGTACTTGTCAAAAATTATCCACAAAACCTTTGTGGAGGTGGATGAAAACGGTACCCAGGCAGCTGCAGCCACTGGGGCTGTTGTCTCGGAAAGGTCACTACGATCTTGGGTGGAGTTTAATGCCAACCACCCTTTTCTCTTTTTCATTAGACACAACAAAACCCAAACCATTCTCTTTTATGGCAGGGTCTGCTCTCCTTAAAAGGGGAGCAGTGTCTAGTACTTTGGAGCTGGAGGAAAANOV23e,Protein SequenceSEQ ID NO: 560365 aaMW at 41476.8kDMDSLVTANTKFCFDLFQEIGKDDRhKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVRSLNNESGLVSCYFGQLLSKLDRIKTDYTLSIANPLYGESSLGDKSETLSQKKKKKIIYTNAFDTIHTQDILWDLFLGKIKELFSKDAINAETVLVLVNAVYFKACWETYFDHENTVDAPFCLNQNENKSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGITYEKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETPADLTGISPSPNLYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYGRVCSP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 23B.

TABLE 23BComparison of the NOV23 protein sequences.NOV23a-MDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHNOV23b-MDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHNOV23cTMDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVLHNOV23d-MDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAAIGMVRLGAISDSAHHIDEVR-NOV23e-MDSLVTANTKFCFDLFQEIGKDDRHKNIFFSPLSLSAALGMVRLGARSDSAHQIDEVR-NOV23aFDEFSQNESKEPDPCLKSNKQKVLADSSLEGQKKTTEPLDQQAGSLNNESGLVSCYFGQLNOV23bFNEFSQNESKEP------------------------------AGSLNNESGLVSCYFGQLNOV23cFNEFSQNESKEPDPCLKSNKQKVLADSSLEGQKKTTEPLDQQAGSLNNESGLVSCYFGQLNOV23d-VHYSQNESKEPDPCLKSNKQKVLADSSLEGQKKTTEPLDQQAGSLNNGSGLVSCYFGQLNOV23e--------------------------------------------SLNNESGLVSCYFGQLNOV23aLSKLDRIKTDYTLSIANRLYGEQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINNOV23bLSKLDRIKTDYTLSIANRLYGEQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINNOV23cLSKLDRIKTDYTLSIANRLYGGQEFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINNOV23dLSKLDRIKTDYTLSIADRLYGEREFPICQEYLDGVIQFYHTTIESVDFQKNPEKSRQEINNOV23eLSKLDRIKTDYTLSIANRLYGESSLGDKSETLS------QKKKKKIIYThAFDTIHTQDINOV23aFWVECQSQGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNANENKNOV23bFWVECQSQGKIKDLFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNQNENKNOV23cFWVECQSQGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNANENKNOV23dFWVECQSQGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNANENKNOV23eLWDLF--LGKIKELFSKDAINAETVLVLVNAVYFKAKWETYFDHENTVDAPFCLNQNENKNOV23aSVKMMMQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYENOV23bSVKMMTQKGLYRIGFIEEVKAQILENRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYENOV23cSVKNMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYENOV23dSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKGLEELERKITYENOV23eSVKMMTQKGLYRIGFIEEVKAQILEMRYTKGKLSMFVLLPSHSKDNLKG-------ITYENOV23aKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLNOV23bKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLNOV23cKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLNOV23dKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLNOV23eKMVAWSSSENMSEESVVLSFPRFTLEDSYDLNSILQDMGITDIFDETRADLTGISPSPNLNOV23aYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYNOV23bYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYNOV23cYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYNOV23dYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIRHNKTQTILFYNOV23eYLSKIIHKTFVEVDENGTQAAAATGAVVSERSLRSWVEFNANHPFLFFIPHNKTQTILFYNOV23aGRVCSPNOV23bGRVCSPNOV23cGRVCSPNOV23dGRVCSPNOV23eGRVCSPNOV23a(SEQ ID NO: 552)NOV23b(SEQ ID NO: 554)NOV23c(SEQ ID NO: 556)NOV23d(SEQ ID NO: 558)NOV23e(SEQ ID NO: 560)


Further analysis of the NOV23a protein yielded the following properties shown in Table 23C.

TABLE 23CProtein Sequence Properties NOV23aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 1; neg. chg 1H-region:length 3; peak value −2.92PSG score:−7.33GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −2.16possible cleavage site: between 40 and 41>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 0PERIPHERALLikelihood = 3.07 (at 197)ALOM score: 0.42 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment (75):7.00Hyd Moment (95):3.76G content:0D/E content:2S/T content:3Score: −6.29Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 10.8%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: cytoplasmicReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):47.8%: mitochondrial30.4%: cytoplasmic17.4%: nuclear 4.3%: vacuolar>> prediction for CG54308-04 is mit (k = 23)


A search of the NOV23a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 23D.

TABLE 23DGeneseq Results for NOV23aNOV23aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG70630Amino acid sequence for novel human1 . . . 425423/425 (99%)0.0thypin - Homo sapiens, 425 aa.1 . . . 425424/425 (99%)[WO200272769-A2, 19-SEP-2002]ABB53280Human polypeptide #20 - Homo 1 . . . 425392/425 (92%)0.0sapiens, 394 aa. [WO200181363-A1,1 . . . 394393/425 (92%)01-NOV-2001]AAM47207Human NOV1 protein - Homo sapiens,1 . . . 425391/425 (92%)0.0395 aa. [WO200174851-A2, 11-OCT-2001]1 . . . 395393/425 (92%)ABG28280Novel human diagnostic protein1 . . . 394388/394 (98%)0.0#28271 - Homo sapiens, 617 aa.109 . . . 502 389/394 (98%)[WO200175067-A2, 11-OCT-2001]ABG70294Human novel polypeptide #10 - Homo 1 . . . 425327/425 (76%)e−172sapiens, 365 aa. [WO200257452-A2,1 . . . 365332/425 (77%)25-JUL-2002]


In a BLAST search of public sequence databases, the NOV23a protein was found to have homology to the proteins shown in the BLASTP data in Table 23E.

TABLE 23EPublic BLASTP Results for NOV23aNOV23aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96P63Serpin B12 - Homo sapiens1 . . . 425403/425 (94%)0.0(Human), 405 aa.1 . . . 405404/425 (94%)CAD13083Sequence 1 from Patent1 . . . 425391/425 (92%)0.0WO0174851 - Homo sapiens1 . . . 395393/425 (92%)(Human), 395 aa.Q9D7P92300003F07Rik protein (Weakly1 . . . 425303/425 (71%)    e−174similar to serpin B12) - Mus1 . . . 423354/425 (83%)musculus (Mouse), 423 aa.I38202leupin precursor - human, 390 aa.1 . . . 425196/426 (46%)   4e−951 . . . 390267/426 (62%)P48594Squamous cell carcinoma antigen 21 . . . 425196/426 (46%)   4e−95(SCCA-2) (Leupin) - Homo sapiens1 . . . 390267/426 (62%)(Human), 390 aa.


PFam analysis predicts that the NOV23a protein contains the domains shown in the Table 23F.

TABLE 23FDomain Analysis of NOV23aIdentities/PfamNOV23a MatchSimilarities forDomainRegionthe Matched RegionExpect Valueserpin1 . . . 425161/441 (37%)1.9e−147350/441 (79%)


Example 24

The NOV24 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 24A.

TABLE 24ANOV24 Sequence AnalysisNOV24a, CG54764-02SEQ ID NO: 561339 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 337ATGAGCCCCTTTGGCAGTCTGGCGAAGCTCTTGGGTCCTTCTCAGATTGCATGGTGGTGCATCACGACCCTGTGCTGTTTTCCAGAGAGGTATGCTGGACGGGACCATAACAGCTGCAAACTCTCCCAGAGGGGGTTCCTAAACTTCATGAACACTGTACTGGTTGCCTTCACAAAGAACCAGAAGGGCTCTGGTGCCCTTGACTGCATGATGAAGAAACTGGACTTCAACTGTGATGGGCAGCTAGATTTTCAGGACTTTCTCAGTCTTACTGATGGTGTAGCTGTGGCTTGCCCTGACTCCTTCATCCCGGCTGGCCATGCCCATGAGAGAATCTGANOV24a, CG54764-02Protein SequenceSEQ ID NO: 562112 aaMW at 12368.1kDMSPFGSLAKLLGPSQIAWWCITTLCCFPERYAGRDHNSCKLSQRGFLNFMNTVLVAFTKNQKGSGALDCMMKKLDFNCDGQLDFQDFLSLTDGVAVACPDSFIPAGHAHERINOV24b, CG54764-01SEQ ID NO: 563348 bpDNA SequenceORF Start: ATG at 5ORF Stop: TGA at 341TGCCATGAGCCCCTTTGGCAGTCTGGCGAAGCTCTTGGGTCCTTCTCAGATTGCATGGTGGTGCATCACGACCTGTGCTGTTTTCCAGAGAGGGTATGCTGGACGGGACCATAACAGCTGCAAACTCTCCCAGAGGGGGTTCCTAAACTTCATGAACACTGTACTGGTTGCCTTCACAAAGAACCAGAAGGGCTCTGGTGCCCTTGACTGCATGATGAAGAAACTGGACTTCAACTGTGATGGGCAGCTAGATTTTCAGGACTTTCTCAGTCTTACTGATGGTGTAGCTGTGGCTTGCCCTGACTCCTTCATCCCGGCTGGCCATGCCCATGAGAGAATCTGAGGTGCNOV24b, 0G54764-01Protein SequenceSEQ ID NO: 564112 aaMW at 12281.0kDMSPFGSLAKLLGPSQIAWWCITTCAVFQRGYAGRDHNSCKLSQRGFLNFMNTVLVAFTKNQKGSGALDCMMKKLDFNCDGQLDFQDFLSLTDGVAVACPDSFIPAGHAHERI


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 24B.

TABLE 24BComparison of the NOV24 protein sequences.NOV24aMSPFGSLAKLLGPSQIAWWCITTLCCFPERYAGRDHNSCKLSQRGFLNFMNTVLVAFTKNNOV24bMSPFGSLAKLLGPSQIAWWCITTCAVFQRGYAGRDHNSCKLSQRGFLNFMNTVLVAFTKNNOV24aQKGSGALDCMMKKLDFNCDGQLDFQDFLSLTDGVAVACPDSFIPAGHAHERINOV24bQKGSGALDCMMKKLDFNCDGQLDFQDFLSLTDGVAVACPDSFIPAGHAHERINOV24a(SEQ ID NO: 562)NOV24b(SEQ ID NO: 564)


Further analysis of the NOV24a protein yielded the following properties shown in Table 24C.

TABLE 24CProtein Sequence Properties NOV24aSignalP analysis:Cleavage site between residues 33 and 34PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 9; pos. chg 1; neg. chg 0H-region:length 19; peak value 9.43PSG score:5.03GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −5.43possible cleavage site: between 28 and 29>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 3.18 (at 87)ALOM score: 3.18 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 6Charge difference: 0.0 C(1.0)-N(1.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment (75):0.38Hyd Moment (95):3.15G content:2D/E content:1S/T content:5Score: −5.58Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.9%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: cytoplasmicReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):43.5%: cytoplasmic21.7%: mitochondrial21.7%: nuclear 4.3%: extracellular, including cell wall 4.3%: vacuolar 4.3%: endoplasmic reticulum>> prediction for CG54764-02 is cyt (k = 23)


A search of the NOV24a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 24D.

TABLE 24DGeneseq Results for NOV24aNOV24aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE16342Human calgizzarin-like-like protein,1 . . . 112106/112(94%)4e−59POLY6 - Homo sapiens, 112 aa.1 . . . 112106/112(94%)[WO200185767-A2, 15-NOV-2001]AAG65905Amino acid sequence of GSK gene Id7 . . . 112100/106(94%)2e−5735812 - Homo sapiens, 106 aa.1 . . . 106104/106(97%)[WO200172961-A2, 04-OCT-2001]AAG65904Amino acid sequence of GSK gene Id20 . . . 108 88/89(98%)2e−4735812 - Homo sapiens, 114 aa.26 . . . 113 88/89(98%)[WO200172961-A2, 04-OCT-2001]AAB58356Lung cancer associated polypeptide6 . . . 11154/106(50%)6e−22sequence SEQ ID 694 - Homo sapiens,29 . . . 133 73/106(67%)134 aa. [WO200055180-A2, 21-SEP-2000]ABG96407Human ovarian cancer marker M471 -7 . . . 11154/105(51%)7e−22Homo sapiens, 105 aa.1 . . . 10472/105(68%)[WO200271928-A2, 19-SEP-2002]


In a BLAST search of public sequence databases, the NOV24a protein was found to have homology to the proteins shown in the BLASTP data in Table 24E.

TABLE 24EPublic BLASTP Results for NOV24aNOV24aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueP50543Calgizzarin (Endothelial monocyte-20 . . . 10352/84(61%)9e−24activating polypeptide) (EMAP) - Mus 8 . . . 91 65/84(76%)musculus (Mouse), 98 aa.P24480Calgizzarin (S100C protein) -20 . . . 11153/92(57%)1e−22Oryctolagus cuniculus (Rabbit), 10210 . . . 10166/92(71%)aa.BAC56502Similar to S100 calcium-binding 7 . . . 11055/104(52%)2e−22protein A11 - Bos taurus (Bovine), 103 1 . . . 10373/104(69%)aa (fragment).P31950Calgizzarin (S100C protein) - Sus 20 . . . 10350/84(59%)2e−21scrofa (Pig), 99 aa.11 . . . 94 63/84(74%)P31949Calgizzarin (S100C protein) (MLN 70) - 7 . . . 11154/105(51%)2e−21Homo sapiens (Human), 105 aa. 1 . . . 10472/105(68%)


PFam analysis predicts that the NOV24a protein contains the domains shown in the Table 24F.

TABLE 24FDomain Analysis of NOV24aIdentities/PfamNOV24aSimilarities forExpectDomainMatch Regionthe Matched RegionValueS_10020 . . . 6011/44 (25%)0.2328/44 (64%)efhand66 . . . 94 7/29 (24%)0.2222/29 (76%)


Example 25

The NOV25 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 25A.

TABLE 25ANOV25 Sequence AnalysisNOV25a, CG55033-04SEQ ID NO: 5651722 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 1591ATGAAGCTGTGGATTCATCTCTTTTATTCATCTCTCCTTGCCTGTATATCTTTACACTCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAACAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATAGATGGAGAGTTTGAGGGCTTTCGCAGAAATGCTGTGATTCTGTTTTAAGTCCATACCTTGTAAATTAGTGCCTTACGTGAGTGTGTCATCCATCAGAACCTAAGCACAGCAGTAAACTATGGAGAAAAAANOV25a, CG55033-04Protein SequenceSEQ ID NO: 566530 aaMW at 59730.7kDMKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRNKIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25b, CG55033-05SEQ ID NO: 5672058 bpSequenceORF Start: ATG at 1ORF Stop: TAG at 1927ATGAAGCTGTGGATTCATCTCTTTTATTCATCTCTCCTTGCCTGTATATCTTTACACTCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGCAGTTAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAACTACCCACCAAAGCACCACAGATTGACCTTGAGAGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGCCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATALAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATAGATGGAGAGTTTGAGGGCTTTCGCAGAAATGCTGTGATTCTGTTTTAAGTCCATACCTTGTAAATTAGTGCCTTACGTGAGTGTGTCATCCATCAGAACCTAAGCACAGCAGTAAACTATGGAGAAAAAANOV25b, CG55033-05Protein SequenceSEQ ID NO: 568642 aaMW at 72606.2kDMKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25c, CG55033-01SEQ ID NO: 5692397 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 2395ATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTGCTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATAGNOV25c, CG55033-01Protein SequenceSEQ ID NO: 570 798 aaMW at 90352.1kDMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRNSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLEAEPDYLEVLEQQTNOV25d, 237376826SEQ ID NO: 571990 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACACTCGAGGCGNOV25d, 237376826Protein SequenceSEQ ID NO: 572330 aaMW at 37853.8kDRGTPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEANOV25e, 310658403SEQ ID NO: 5731648 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCTCGCGAACCATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGAAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCGTCGACGGCNOV25e, 310658403Protein SequenceSEQ ID NO: 574549 aaMW at 61376.4kDTSRTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTVDGNOV25f, 317980876SEQ ID NO: 5751887 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTATAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTAACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTACCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACAAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACACTCGAGGCGNOV25f, 317980876Protein SequenceSEQ ID NO: 576629 aaMW at 71406.5kDRGTKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKILQRSLLEQENHSPLTGSNNKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEANOV25g, 318018151SEQ ID NO: 5771887 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCCCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGAGCCTCCTCCGCAAAATCCTAGAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTTACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACACTCGAGGCGNOV25g, 318018151Protein SequenceSEQ ID NO: 578629 aaMW at 71406.5kDRGTKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQPSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITVIFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEXTLEQQTLEANOV25h, 318176316SEQ ID NO: 5792433 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCACCATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGACAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCACCTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAGCTTATCAGGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTACCCAGATTGACCTTGAGTGAAGAAGGATCGTTTACGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAGAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACAGAACCATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAGCAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACACTCGAGGCGAAGGGCGAATTCCAGCACNOV25h, 318176316Protein SequenceSEQ ID NO: 580811 aaMW at 91777.6kDRGTTMLINCEAKGIKMVSIESVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFTNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYTMVTTPATTTNTADTILRSLTDAVPLSVTILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEAKGEFQHNOV25i, CG55033-02SEQ ID NO: 5811668 bpDNA SequenceORF Start: ATG at 1ORE Stop: at 1627ATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAATATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCATACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTGCTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGANOV25i, CG55033-02Protein SequenceSEQ ID NO: 582542 aaMW at 60659.6kDMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRXLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTNOV25j, CG55033-03SEQ ID NO: 5831668 bpDNA SequenceORF Start: ATG at 1ORF Stop: end of sequenceATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATATCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAGGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACCTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATCTGTCCAQGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGANOV25j,CG55033-03SequenceSEQ ID NO: 584556 aaMW at 62099.2kDMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIFAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNPLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRISTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRNOV25k, CG5503306SEQ ID NO: 5852539 bpDNA SequenceORF Start: ATG at 5ORF Stop: TAG at 2528CAACATGAAGCTGTGGATTCATCTCTTTTATTCATCTCTCCTTGCCTGTATATCTTTACACTCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATAGATGGAGAGTNOV25k, CG55033-06Protein SequenceSEQ ID NO: 586841 aaMW at 95108.5kDMKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKSLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRPRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLVELEQQTNOV25l, CG55033-07SEQ ID NO: 5871648 bpDNA SequenceORF Start: ATG at 14ORF Stop: at 1640CACCTCGCGAACCATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCGTCGACGGCNOV25l, CG55033-07Protein SequenceSEQ ID NO: 588542 aaMW at 60659.6kDMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKTLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTNOV25m, CG55033-08SEQ ID NO: 589643 bpDNA SequenceORF Start: ATG at 9ORF Stop: at 1635GCGGCCGCATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAGGAAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCCTCGAGGGCNOV25m, CG55033-08Protein SequenceSEQ ID NO: 590542 aaMW at 60659.6kDMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSETLCPGLVNNPSMPTQTSYLMVTNOV25n, CG55033-09SEQ ID NO: 5912838 bpDNA SequenceORF Start: ATG at 184ORF Stop: TAG at 2707AACTTTATGAAGCTATGGGACTTGACAAAAAGTGATATTTGAGAAGAAAGTACGCAGTGGTTGGTGTTTTCTTTTTTTTAATAAAGGAATTGAATTACTTTGAACACCTCTTCCAGCTGTGCATTACAGATAACGTCAGGAAGAGTCTCTGCTTTACAGAATCGGATTTCATCACATGACAACATGAAGCTGTGGATTCATCTCTTTTATTCATCTCTCCTTGCCTGTATATCTTTACACTCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCCTCGACAAAAAGGAATTGAAAGCCCTALATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATAGATGGAGAGTATGAGGGCTTTCGCAGAAATGCTGTGATTCTGTTTTAAGTCCATACCTTGTAAATTAGTGCCTTACGTGAGTGTGTCATCCATCAGAACCTAAGCACAGCAGTAAACTATGGAGAAAAAANOV25n, CG55033-09Protein SequenceSEQ ID NO: 592841 aaMW at 95108.5kDMKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFNGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25o, CG55033-10SEQ ID NO: 5932531 bpDNA SequenceORF Start: at 2ORF Stop: TAA at 2513GGATTCTCTCTTTTATTCATCTCTCCTTGCCTGTATATCTTTACACTCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCTACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACAATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAATGCTTCACTTGGGAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAAAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTGCTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAATCACAGAATACCTAAGGAAAAAACATTGCTCAGCTCCAGCCTGATATGGAGGCACATTATCCTGGAGCCACGAAGAGCTGAAGTTAATGGAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATAAGGGCGAATTCTGCTGTNOV25o, CG55033-10Protein SequenceSEQ ID NO: 594837 aaMW at 94517.6kDDSLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVLPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25p, CG55033-11SEQ ID NO: 5951758 bpDNA SequenceORF Start: at 10ORF Stop: at 1750CGCGGATCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTAGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAGAAGGAATCTAATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGAGCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTALATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTCGAGGCGNOV25p, CG55033-11Protein SequenceSEQ ID NO: 596580 aaMW at 64615.9kDQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSNOV25q, CG55033-12SEQ ID NO: 5971762 bpDNA SequenceORF Start: at 14ORF Stop: at 1754CACCTCGCGAACCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTGTCGACGGCNOV25q, CG55033-12Protein SequenceSEQ ID NO: 598580 aaMW at 64615.9kDQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINhNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSNOV25r, CG55033-13SEQ ID NO: 5992481 bpDNA SequenceORF Start: at 10ORF Stop: at 2473CGCGGATCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATAAATTACAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTACAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCATCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAAACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGACATTAATGTACTCACGTCACAAGGAAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACACTCGAGGCGNOV25r, CG55033-13Protein SequenceSEQ ID NO: 600821 aaMW at 92763.6kDQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSPLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRNSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25s, CG55033-14SEQ ID NO: 6012548 bpDNA SequenceORF Start: ATG at 14ORF Stop: TGA at 2537CACCGGTACCACCATGAAGCTGTGGATTCATCTCTTTTATTCATCTCTCCTTGCCTGTATATCTTTACACTCCCAAACTCCAGTGCTCTCATCCAGAGGCTCTTGTGATTCTCTTTGCAATTGTGAGGAAAAAGATGGCACAATGCTAATAAATTGTGAAGCAAAAGGTATCAAGATGGTATCTGAAATAAGTGTGCCACCATCACGACCTTTCCAACTAAGCTTATTAAATAACGGCTTGACGATGCTTCACACAAATGACTTTTCTGGGCTTACCAATGCTATTTCAATACACCTTGGATTTAACAATATTGCAGATATTGAGATAGGTGCATTTAATGGCCTTGGCCTCCTGAAACAACTTCATATCAATCACAATTCTTTAGAAATTCTTAAAGAGGATACTTTCCATGGACTGGAAAACCTGGAATTCCTGCAAGCAGATAACAATTTTATCACAGTGATTGAACCAAGTGCCTTTAGCAAGCTCAACAGACTCAAAGTGTTAATTTTAAATGACAATGCTATTGAGAGTCTTCCTCCAAACATCTTCCGATTTGTTCCTTTAACCCATCTAGATCTTCGTGGAAATCAATTACAAACATTGCCTTATGTTGGTTTTCTCGAACACATTGGCCGAATATTGGATCTTCAGTTGGAGGACAACAAATGGGCCTGCAATTGTGACTTATTGCAGTTAAAAACTTGGTTGGAGAACATGCCTCCACAGTCTATAATTGGTGATGTTGTCTGCAACAGCCCTCCATTTTTTAAAGGAAGTATACTCAGTAGACTAAAGAAGGAATCTATTTGCCCTACTCCACCAGTGTATGAAGAACATGAGGATCCTTCAGGATCATTACATCTGGCAGCAACATCTTCAATAAATGATAGTCGCATGTCAACTAAGACCACGTCCATTCTAAAACTACCCACCAAAGCACCAGGTTTGATACCTTATATTACAAAGCCATCCACTCAACTTCCAGGACCTTACTGCCCTATTCCTTGTAACTGCAAAGTCCTATCCCCATCAGGACTTCTAATACATTGTCAGGAGCGCAACATTGAAAGCTTATCAGATCTGAGACCTCCTCCGCAAAATCCTAGAAAGCTCATTCTAGCGGGAAATATTATTCACAGTTTAATGAAGTCTGATCTAGTGGAATATTTCACTTTGGAAATGCTTCACTTGGGAAACAATCGTATTGAAGTTCTTGAAGAAGGATCGTTTATGAACCTAACGAGATTGCAAAAACTCTATCTAAATGGTAACCACCTGACCAAATTAAGTAAAGGCATGTTCCTTGGTCTCCATAATCTTGAATACTTATATCTTGAATACAATGCCATTAAGGAAATACTGCCAGGAACCTTTAATCCAATGCCTAAACTTAAAGTCCTGTATTTAAATAACAACCTCCTCCAAGTTTTACCACCACATATTTTTTCAGGGGTTCCTCTAACTAAGGTAAATCTTAAAACAAACCAGTTTACCCATCTACCTGTAAGTAATATTTTGGATGATCTTGATTTACTAACCCAGATTGACCTTGAGGATAACCCCTGGGACTGCTCCTGTGACCTGGTTGGACTGCAGCAATGGATACAAAAGTTAAGCAAGAACACAGTGACAGATGACATCCTCTGCACTTCCCCCGGGCATCTCGACAAAAAGGAATTGAAAGCCCTAAATAGTGAAATTCTCTGTCCAGGTTTAGTAAATAACCCATCCATGCCAACACAGACTAGTTACCTTATGGTCACCACTCCTGCAACAACAACAAATACGGCTGATACTATTTTACGATCTCTTACGGACGCTGTGCCACTGTCTGTTCTAATATTGGGACTTCTGATTATGTTCATCACTATTGTTTTCTGTGCTGCAGGGATAGTGGTTCTTGTTCTTCACCGCAGGAGAAGATACAAAAAGAAACAAGTAGATGAGCAAATGAGAGACAACAGTCCTGTGCATCTTCAGTACAGCATGTATGGCCATAAAACCACTCATCACACTACTGAAAGACCCTCTGCCTCACTCTATGAACAGCACATGGTGAGCCCCATGGTTCATGTCTATAGAAGTCCATCCTTTGGTCCAAAGCATCTGGAAGAGGAAGAAGAGAGGAATGAGAAAGAAGGAAGTGATGCAAAACATCTCCAAAGAAGTCTTTTGGAACAGGAAAATCATTCACCACTCACAGGGTCAAATATGAAATACAAAACCACGAACCAATCAACAGAATTTTTATCCTTCCAAGATGCCAGCTCATTGTACAGAAACATTTTAGAAAAAGAAAGGGAACTTCAGCAACTGGGAAATCACAGAATACCTAAGGAAAAACATTGCTCAGCTCCAGCCTGATATGGAGAACATTATCCTGGAGCCCACGAAGAGCTGAAGTTAATGGAAACATTAATGTACTCACGTCCAAGGAAGGTATTAGTGGAACAGACAAAAAATGAGTATTTTGAACTTAAAGCTAATTTACATGCTGAACCTGACTATTTAGAAGTCCTGGAGCAGCAAACATGACTCGAGGGCNOV25s, CG55033-14Protein SequenceSEQ ID NO: 6021841 aaMW at 95108.5kDMKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYLYEYNAIKEILPGATFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQT


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 25B.

TABLE 25BComparison of the NOV25 protein sequences.NOV25a ------------------------------------------------------------NOV25b MKLWIHLFYSSLLACISLHSQTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKIMVSEISNOV25c ------------------------------------------------------------NOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ----------------RGTKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSNOV25g ----------------RGTKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSNOV25h ------------------------------------------------------------NOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k ------------------------------------------------------------NOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ------------------------------------------------------------NOV25o ------------------------------------------------------------NOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ------------------------------------------------------------NOV25s ------------------------------------------------------------NOV25a ----------------------------------------MKLWIHLFYSSLLACISLHSNOV25b VPPSRPFQLSLLNNGLTMLHTNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINNOV25c ------------------------------------------------------------NOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f RLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKNOV25g RLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKNOV25h RLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKNOV25i RLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKNOV25j RLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKNOV25k ----------------------------------------MKLWIHLFYSSLLACISLHSNOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ----------------------------------------MKLWIHLFYSSLLACISLHSNOV25o --------------------------------------------DSLFYSSLLACISLHSNOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ------------------------------------------------------------NOV25s ----------------------------------------MKLWIHLFYSSLLACISLHSNOV25a QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25b HNSLEILKEDTFHGLENLEFLQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFNOV25c -----------------------MLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25d ------------------------------------------------------------NOV25e -------------------TSRTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25f PSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMNOV25g PSTQLPGPYCPIPCNCKVLSPSGLLIHCQERNIESLSDLRPPPQNPRKLILAGNIIHSLMNOV25h -------------------RGTTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25i -----------------------MLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25j -----------------------MLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25k QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25l -----------------------MLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25m -----------------------MLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25n QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMIHNOV25o QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVLPSRPFQLSLLNNGLTMLHNOV25p QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25q QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25r QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25s QTPVLSSRGSCDSLCNCEEKDGTMLINCEAKGIKMVSEISVPPSRPFQLSLLNNGLTMLHNOV25a TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25b RFVPLTHLDLRGNQLQTLPYVGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSNOV25c TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25d ------------------------------------------------------------NOV25e TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25f KSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYNOV25g KSDLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMFLGLHNLEYNOV25h TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25i TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25j TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINNNSLEILKEDTFHGLENLEFNOV25k TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25l TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25m TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25n TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25o TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25p ThDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25q TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINNNSLEILKEDTFHGLENLEFNOV25r TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25s TNDFSGLTNAISIHLGFNNIADIEIGAFNGLGLLKQLHINHNSLEILKEDTFHGLENLEFNOV25a LQADNNFITVIEPSAFSKLNRLKVLILNDNATESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25b IIGDVVCNSPPFFKGSILSRLKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTNOV25c LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25d ------------------------------------------------------------NOV25e LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25f LYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVNOV25g LYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNQFTHLPVNOV25h LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25i LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25j LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25k LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25l LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25m LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25n LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25o LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25p LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25q LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25r LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25s LQADNNFITVIEPSAFSKLNRLKVLILNDNAIESLPPNIFRFVPLTHLDLRGNQLQTLPYNOV25a VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25b TSILKLPTKAPQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKANOV25c VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25d ---------------RGTPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKANOV25e VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25f SNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKANOV25g SNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHLDKKELKANOV25h VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25i VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25j VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25k VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25l VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25m VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25n VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25o VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25p VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25q VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25r VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25s VGFLEHIGRILDLQLEDNKWACNCDLLQLKTWLENMPPQSIIGDVVCNSPPFFKGSILSRNOV25a LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25b LNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFINOV25c LKKESICPTPPVYEEHEDPSGSLHIAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25d LNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFINOV25e LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25f LNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFINOV25g LNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLILGLLIMFINOV25h LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25i LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25j LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRISTKTTSILKLPTKAPGLIPYITKPNOV25k LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25l LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25m LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25n LKKESICPTPPVYEEHEDPSGSLHTAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25o LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25p LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25q LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25r LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25s LKKESICPTPPVYEEHEDPSGSLHLAATSSINDSRMSTKTTSILKLPTKAPGLIPYITKPNOV25a STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25b TIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYENOV25c STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25d TIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYENOV25e STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25f TIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYENOV25g TIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTERPSASLYENOV25h STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25i STQLPGPYCPIPCNCKVLSPSGLLIHCQEPN---------IESLSDLRPPPQNPRKLILANOV25j STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25k STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25l STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25m STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25n STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRXLILANOV25o STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25p STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25q STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25r STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25s STQLPGPYCPIPCNCKVLSPSGLLIHCQERN---------IESLSDLRPPPQNPRKLILANOV25a GNIIHSLMK---------------------------------------------------NOV25b QHMVSPMVHVYR------------------------------------------------NOV25c GNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFNNLTRLQKLYLNGNHLTKLSKGMFNOV25d QHMVSPMVHVYR------------------------------------------------NOV25e GNIIHSLMKS--------------------------------------------------NOV25f QHMVSPMVHVYR------------------------------------------------NOV25g QHMVSPMVHVYR------------------------------------------------NOV25h GNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFTNLTRLQKLYLNGNHLTKLSKGMFNOV25i GNIIHSLNKS--------------------------------------------------NOV25j GNIIHSLMKS--------------------------------------------------NOV25k GNIIHSLMKSDLVEYFTLEMLHLGNNRIEVLEEGSFTNLTRLQKLYLNGNHLTKLSKGMFNOV25l GNIIHSLMKS--------------------------------------------------NOV25m GNIIHSLMKS--------------------------------------------------NOV25n GNIIHSLMKS--------------------------------------------------NOV25o GNIIHSLMKS--------------------------------------------------NOV25p GNIIHSLMKS--------------------------------------------------NOV25q GNIIHSLMKS--------------------------------------------------NOV25r GNIIHSLMKS--------------------------------------------------NOV25s GNIIHSLMKS--------------------------------------------------NOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c LGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h LGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k LGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKTNOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ------------------------------------------------------------NOV25o ------------------------------------------------------------NOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ------------------------------------------------------------NOV25s ------------------------------------------------------------NOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c NQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHNOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h NQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHNOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k NQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGHNOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ------------------------------------------------------------NOV25o ------------------------------------------------------------NOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ------------------------------------------------------------NOV25s ------------------------------------------------------------NOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c LDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLINOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h LDKKELKALNSEILCPGLVNNPSMPTQTSYLNVTTPATTTNTADTILRSLTDAVPLSVLINOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k LDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLINOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ------------------------------------------------------------NOV25o ------------------------------------------------------------NOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ------------------------------------------------------------NOV25s ------------------------------------------------------------NOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c LGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTENOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h LGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTENOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k LGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTENOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ------------------------------------------------------------NOV25o ------------------------------------------------------------NOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ------------------------------------------------------------NOV25s ------------------------------------------------------------NOV25a --------------------SPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25b --------------------SPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25c RPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25d --------------------SPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25e -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25f --------------------SPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25g --------------------SPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25h RPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25i -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25j -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25k RPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEE---RN----EKEGSDAKHLQRSLLEQNOV25l -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25m -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25n -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25o -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25p -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25q -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25r -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25s -----------DLVEYFTLEMLHLGNNRIEVLEEGSFMNLTRLQKLYLNGNHLTKLSKGMNOV25a EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKNOV25b EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKNOV25c EN----------------------HSPLTGSNNKYKTTNQSTEFLSFQDASSLYRNILEKNOV25d EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYENILEKNOV25e FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25f EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKNOV25g EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKNOV25h EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKNOV25i FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25j FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25k EN----------------------HSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKNOV25l FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNMCNOV25m FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25n FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25o FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25p FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25q FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25r FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSOVPLTKVNLKNOV25s FLGLHNLEYLYLEYNAIKEILPGTFNPMPKLKVLYLNNNLLQVLPPHIFSGVPLTKVNLKNOV25a ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25b ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25c ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25d ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25e TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25f ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25g ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25h ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25i TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25j TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25k ERELQQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPR-------------NOV25l TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25m TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25n TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25o TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25p TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25q TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25r TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25s TNQFTHLPVSNILDDLDLLTQIDLEDNPWDCSCDLVGLQQWIQKLSKNTVTDDILCTSPGNOV25a -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQT---------------------NOV25b -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQT---------------------NOV25c -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQT---------------------NOV25d -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEA------------------NOV25e HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTVDG----------------------NOV25f -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEA------------------NOV25g -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEA------------------NOV25h -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQTLEAKGEFQH------------NOV25i HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMBT-------------------------NOV25j HLDKKELKALNSETLCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILR-----------NOV25k -------KVLVEQTKNEYFELKANLHAEPDYLEVLEQQT---------------------NOV25l HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVT-------------------------NOV25m HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVT-------------------------NOV25n HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLNOV25o HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLNOV25p HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRS----------NOV25q HLDKKELKALNSETLCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRS----------NOV25r HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLNOV25s HLDKKELKALNSEILCPGLVNNPSMPTQTSYLMVTTPATTTNTADTILRSLTDAVPLSVLNOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c ------------------------------------------------------------NOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h ------------------------------------------------------------NOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k ------------------------------------------------------------NOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTNOV25o ILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTNOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTNOV25s ILGLLIMFITIVFCAAGIVVLVLHRRRRYKKKQVDEQMRDNSPVHLQYSMYGHKTTHHTTNOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c ------------------------------------------------------------NOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h ------------------------------------------------------------NOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k ------------------------------------------------------------NOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n ERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLNOV25o ERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLNOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r ERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLNOV25s ERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKHLQRSLLEQENHSPLNOV25a ------------------------------------------------------------NOV25b ------------------------------------------------------------NOV25c ------------------------------------------------------------NOV25d ------------------------------------------------------------NOV25e ------------------------------------------------------------NOV25f ------------------------------------------------------------NOV25g ------------------------------------------------------------NOV25h ------------------------------------------------------------NOV25i ------------------------------------------------------------NOV25j ------------------------------------------------------------NOV25k ------------------------------------------------------------NOV25l ------------------------------------------------------------NOV25m ------------------------------------------------------------NOV25n TGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYNOV25o TGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYNOV25p ------------------------------------------------------------NOV25q ------------------------------------------------------------NOV25r TGSNNKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYNOV25s TGSNMKYKTTNQSTEFLSFQDASSLYRNILEKERELQQLGITEYLRKNIAQLQPDMEAHYNOV25a ---------------------------------------------------NOV25b ---------------------------------------------------NOV25c ---------------------------------------------------NOV25d ---------------------------------------------------NOV25e ---------------------------------------------------NOV25f ---------------------------------------------------NOV25g ---------------------------------------------------NOV25h ---------------------------------------------------NOV25i ---------------------------------------------------NOV25j ---------------------------------------------------NOV25k ---------------------------------------------------NOV25l ---------------------------------------------------NOV25m ---------------------------------------------------NOV25n PGAHEELKLMETLMYSRPRKVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25o PGAHEELKLMETLMYSRPRXVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25p ---------------------------------------------------NOV25q ---------------------------------------------------NOV25r PGAHEELKLMETLMYSRPRXVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25s PGAHEELKLMETLMYSRPRXVLVEQTKNEYFELKANLHAEPDYLEVLEQQTNOV25a (SEQ ID NO: 566)NOV25b (SEQ ID NO: 568)NOV25c (SEQ ID NO: 570)NOV25d (SEQ ID NO: 572)NOV25e (SEQ ID NO: 574)NOV25f (SEQ ID NO: 576)NOV25g (SEQ ID NO: 578)NOV25h (SEQ ID NO: 580)NOV25i (SEQ ID NO: 582)NOV25j (SEQ ID NO: 584)NOV25k (SEQ ID NO: 586)NOV25l (SEQ ID NO: 588)NOV25m (SEQ ID NO: 590)NOV25n (SEQ ID NO: 592)NOV25o (SEQ ID NO: 594)NOV25p (SEQ ID NO: 596)NOV25q (SEQ ID NO: 598)NOV25r (SEQ ID NO: 600)NOV25s (SEQ ID NO: 602)


Further analysis of the NOV25a protein yielded the following properties shown in Table 25C.

TABLE 25CProtein Sequence Properties NOV25aSignalP analysis:Cleavage site between residues 21 and 22PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 2; pos. chg 1; neg. chg 0H-region:length 25; peak value 8.53PSG score: 4.13GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −3.75possible cleavage site: between 15 and 16>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 1.48 (at 3)ALOM score: 1.48 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 6Charge difference: −2.0 C(0.5) − N(2.5)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):5.82Hyd Moment(95):9.48G content:1D/E content:1S/T content:8Score: −1.79Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 38 SRG|SCNUCDISC: discrimination of nuclear localization signalspat4: RPRK (4) at 496pat7: nonebipartite: nonecontent of basic residues: 9.2%NLS Score: −0.22KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regions386 K0.97387 H0.98388 L0.98389 E0.98390 E0.98391 E0.98392 E0.98393 E0.98394 R0.98395 N0.98396 E0.98397 K0.98398 E0.98399 G0.98400 S0.98401 D0.98402 A0.98403 K0.98404 H0.98405 L0.98406 Q0.98407 R0.98408 S0.98409 L0.98410 L0.98411 E0.98412 Q0.98413 E0.98414 N0.98415 H0.98416 S0.83total: 31 residuesFinal Results (k = 9/23):52.2%: nuclear26.1%: extracellular, including cell wall21.7%: mitochondrial>> prediction for CG55033-04 is nuc (k = 23)


A search of the NOV25a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 25D.

TABLE 25DGeneseq Results for NOV25aNOV25aIdentities/Residues/Similarities forGeneseqProtein/Organism/Length [Patent #,Matchthe MatchedExpectIdentifierDate]ResiduesRegionValueABB53270Human polypeptide #10 - Homo1 . . . 473401/480 (83%)0.0sapiens, 841 aa. [WO200181363-A1,1 . . . 475412/480 (85%)01-NOV-2001]AAE22222Human toll like receptor like molecule 51 . . . 473401/480 (83%)0.0(TLR-L5) protein - Homo sapiens, 8411 . . . 475412/480 (85%)aa. [WO200220569-A2, 14-MAR-2002]ABJ01956158P1D7 SSH protein sequence SEQ1 . . . 473401/480 (83%)0.0ID No 657 - Unidentified, 841 aa.1 . . . 475412/480 (85%)[WO200216593-A2, 28-FEB-2002]ABJ05539Breast cancer-associated protein 4 -1 . . . 473401/480 (83%)0.0Unidentified, 841 aa. [WO200259377-1 . . . 475412/480 (85%)A2, 01-AUG-2002]ABG78359Human protein, homologous to IGFBP-1 . . . 473401/480 (83%)0.0ALS, designated NOV1a - Homo1 . . . 475412/480 (85%)sapiens, 841 aa. [WO200226826-A2,04-APR-2002]


In a BLAST search of public sequence databases, the NOV25a protein was found to have homology to the proteins shown in the BLASTP data in Table 25E.

TABLE 25EPublic BLASTP Results for NOV25aNOV25aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueCAD42380Sequence 656 from Patent1 . . . 473401/480(83%)0.0WO0216593 - Homo sapiens1 . . . 475412/480(85%)(Human), 841 aa.Q8BLLOHypothetical cysteine-rich flanking1 . . . 381339/381(88%)0.0region - Mus musculus (Mouse),1 . . . 380358/381(92%)425 aa.Q8C110Hypothetical cysteine-rich flanking1 . . . 381339/381(88%)0.0region - Mus musculus (Mouse),1 . . . 380358/381(92%)840 aa.Q9H5Y7Hypothetical protein FLJ22774 -44 . . . 381 338/338(100%)0.0Homo sapiens (Human), 440 aa.1 . . . 338338/338(100%)BAC67207Neuronal transmembrane protein1 . . . 468189/491(38%)2e−85Slitrk4 - Mus musculus (Mouse),30 . . . 493 265/491(53%)866 aa.


PFam analysis predicts that the NOV25a protein contains the domains shown in the Table 25F.

TABLE 25FDomain Analysis of NOV25aIdentities/SimilaritiesPfamNOV25afor theExpectDomainMatch RegionMatched RegionValueLRR137 . . . 16010/25 (40%)0.1319/25 (76%)LRRCT218 . . . 26817/54 (31%)2.6e−0839/54 (72%)


Example 26

The NOV26 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 26A.

TABLE 26ANOV26 Sequence AnalysisNOV26a, CG55117-04SEQ ID NO: 6033766 bpDNA SequenceORF Start: ATG at 10ORF Stop: TGA at 2605TTGCTAGCTATGGCCCTCGTACTCGGCTCCCTGTTGCTGCTGGGGCTGTGCGGGAACTCCTTTTCAGGAGGGCAGCCTTCATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCATAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGATTTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGCCAGAAACTGTAATCTTAGGTCTAAAGATTGTCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCTGCTGTTTATTATTCTGATGCCTCTGGTGAAGTATTTCTTTTGTATGTGTCGTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAAAATGGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTATAATAATAAGCATTGGCATCTTCTATGGTTTTGTGGCAAATCACCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGCCCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCCACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGGACAGATTTGGATGGCCTGGTCCAACAGGGCTATCAATCCCTTAATGATATACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCAAAAGGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTTCCTATTCAGGATATACTCTCAGCATTCTCTGTTTATGTTAATAACACTGAAAGTTACATCCACAGAAATTTACCTACATTGGAAGAGTATGATTCATACTGGTGGCTGGGTGGCCTGGTCATCTGCTCTCTGCTGACCCTCATCGTGATTTTTTACTACCTGGGCTTACTGTGTGGCGTGTGCGGCTATGACAGGCATAACACCCCGACCACCCGAGGCTGTGTCTCCAACACCGGAGGCGTCTTCCTCATGGTTGGAGTTGGATTAAGTTTCCTCTTTTGCTGGATATTGATGATCATTGTGGTTCTTACCTTTGTCTTTGGTGCAAATGTGGAAAAACTGATCTGTGAACCTTACACGAGCAAGGAATTATTCCGGGTTTTGGATACACCCTACTTACTAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCTATTTAATAAATCAAAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAGAGGCACTTACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAACATCTCAACATTAATGAGCATACTGGAAGCATAAGCAGTGAATTGGAAAGTCTGAAGGTAAATCTTAATATCTTTCTGTTGGGTGCAGCAGGAAGAAAAAACCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGAATTATGACAGCTACTTGGCTCAGACTGGTAAATCCCCCGCAGGAGTGAATCTTTTATCATTTGCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCCCAGGAAATTTGAGGAACTCCCTGAAAAGAGATGCACAAACTATTAAACAATTCACCAGCAACGAGTCCTTCCTATAGAACAATCACTGAGCACTCTATACCAAAAGCGTCAAGATACTTCAACGCACAGGGAATGGATTGTTGGAGAGAGTAACTAGGATTCTAGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCTCTGTTATTATTGAGGAAACTAAGAAGTATGGGAGAACAATAATAGGATATTTTGAACATTATCTGCAGTGGATCGAGTTCTCTATCAGTGAGAAAGTGGCATCGTGCAAACCTGTGGCCACCGCTCTAGATACTGCTGTTGATGTCTTTCTGTGTAGCTACATTATCGACCCCTTGAATTTGTTTTGGTTTGGCATAGGAAAAGCTACTGTATTTTTACTTCCGGCTCTAATTTTTGCGGTAAAACTGGCTAAGTACTATCGTCGAATGGATTCGGAGGACGTGTACGATGATGTTGAAACTATACCCATGAAAAATATGGAAAATGGTAATAATGGTTATCATAAAGATCATGTATATGGTATTCACAATCCTGTTATGACAAGCCCATCACAACATTGATAGCTGATGTTGAAACTGCTTGAGCATCAGGATACTCAAAGTGGAAAGGATCACAGATTTTTGGTAGTTTCTGGGTCTACAAGGACTTTCCAAATCCAGGAGCAACGCCAGTGGCAACGTAGTGACTCAGGCGGGCACCAAGGCAACGGCACCATTGGTCTCTGGGTAGTGCTTTAAGAATGAACACAATCACGTTATAGTCCATGGTCCATCACTATTCAAGGATGACTCCCTCCCTTCCTGTCTATTTTTGTTTTTTACTTTTTTACACTGAGTTTCTATTTAGACACTACAACATATGGGGTGTTTGTTCCCATTGGATGCATTTCTATCAAAACTCTATCAAATGTGATGGCTAGATTCTAACATATTGCCATGTGTGGAGTGTGCTGAACACACACCAGTTTACAGGAAAGATGCATTTTGTGTACAGTAAACGGTGTATATACCTTTTGTTACCACAGAGTTTTTTAAACAAATGAGTATTATAGGACTTTCTTCTAAATGAGCTAAATAAGTCACCATTGACTTCTTGGTGCTGTTGAAAATAATCCATTTTCACTAAAAGTGTGTGAAACCTACAGCATATTCTTCACGCAGAGATTTTCATCTATTATACTTTATCAAAGATTGGCCATGTTCCACTTGGAAATGGCATGCAAAAGCCATCATAGAGAAACCTGCGTAACTCCATCTGACAAATTCAAAAGAGAGAGAGAGATCTTGAGAGAGAAATGCTGTTCGTTCAAAAGTGGAGTTGTTTTAACAGATGCCAATTACGGTGTACAGTTTAACAGAGTTTTCTGTTGCATTAGGATAAACATTAATTGGAGTGCAGCTAACATGAGTATCATCAGACTAGTATCAAGTGTTCTAAAATGAAATATGAGAAGATCCTGTCACAATTCTTAGATCTGGTGTCCAGCATGGATGAAACCTTTGAGTTTGGTCCCTAAATTTGCATGAAAGCACAAGGTAAATATTCATTTGCTTCAGGAGTTTCATGTTGGATCTGTCATTATCAAAAGTGATCAGCAATGAAGAACTGGTCGGACAAAATTTAACGTTGATGTAATGGAATTCCAGATGTAGGCATTCCCCCCAGGTCTTTTCATGTGCAGATTGCAGTTCTGATTCATTTGAATAAAAAGGAACTTGGNOV26a, CG55117-04SEQ ID NO: 604865 aaMW at 97200.8kDProtein SequenceMALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPATNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKPETVILGLKIVYYEAGIILCCVLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKWAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQHNOV26b, CG55117-01SEQ ID NO: 6053825 bpDNA SequenceORF Start: ATG at 69ORF Stop: TGA at 2664GNNNNNNANNNNATTCNTNCANTGNACNNNACCAAGTTCTACCTCATGTTTGGAGGATCTTGCTAGCTATGGCCCTCGTACTCGGCTCCCTGTTGCTGCTGGGGCTGTGCGGGAACTCCTTTTCAGGAGGGCAGCCTTCATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCATAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGATTTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGCCAGAAACTGTAATCTTAGGTCTAAAGATTGTCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCTGCTGTTTATTATTCTGATGCCTCTGGTGGGGTATTTCTTTTGTATGTGTCGTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAAAATGGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTATAATAATAAGCATTGGCATCTTCTATGGTTTTGTGGCAAATCACCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGCCCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCCACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGGACAGATTTGGATGGCCTGGTCCAACAGGGCTATCAATCCCTTAATGATATACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCAAAAGGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTTCCTATTCAGGATATACTCTCAGCATTCTCTGTTTATGTTAATAACACTGAAAGTTACATCCACAGAAATTTACCTACATTGGAAGAGTATGATTCATACTGGTGGCTGGGTGGCCTGGTCATCTGCTCTCTGCTGACCCTCATCGTGATTTTTTACTACCTGGGCTTACTGTGTGGCGTGTGCGGCTATGACAGGCATGCCACCCCGACCACCCGAGGCTGTGTCTCCAACACCGGAGGCGTCTTCCTCATGGTTGGAGTTGGATTAAGTTTCCTCTTTTGCTGGATATTGATGATCATTGTGGTTCTTACCTTTGTCTTTGGTGCAAATGTGGAAAAACTGATCTGTGAACCTTACACGAGCAAGGAATTATTCCGGGTTTTGGATACACCCTACTTACTAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCTATTTAATAAATCAAAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAGAGGCACTTACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAACATCTCAACATTAATGAGCATACTGGAAGCATAAGCAGTGAATTGGAAAGTCTGAAGGTAAATCTTAATATCTTTCTGTTGGGTGCAGCAGGAAGAAAAAACCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGAATTATGACAGCTACTTGGCTCAGACTGGTAAATCCCCCGCAGGAGTGAATCTTTTATCATTTGCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCCCAGGAAATTTGAGGAACTCCCTGA~AAGAGATGCACAAACTATTAAAACAATTCACCAGCAACGAGTCCTTCCTATAGAACAATCACTGAGCACTCTATACCAAAGCGTCAAGATACTTCAACGCACAGGGAATGGATTGTTGGAGAGAGTAACTAGGATTCTAGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCTCTGTTATTATTGAGGAAACTAAGAAGTATGGGAGAACAATAATAGGATATTTTGAACATTATCTGCAGTGGATCGAGTTCTCTATCAGTGAGAAAGTGGCATCGTGCAAACCTGTGGCCACCGCTCTAGATACTGCTGTTGATGTCTTTCTGTGTAGCTACATTATCGACCCCTTGAATTTGTTTTGGTTTGGCATAGGAAAAGCTACTGTATTTTTACTTCCGGCTCTAATTTTTGCGGTAAAACTGGCTAAGTACTATCGTCGAATGGATTCGGAGGACGTGTACGATGATGTTGAAACTATACCCATGAAAAATATGGAAAATGGTAATAATGGTTATCATAAAGATCATGTATATGGTATTCACAATCCTGTTATGACAAGCCCATCACAACATTGATAGCTGATGTTGAAACTGCTTGAGCATCAGGATACTCAAAGTGGAAAGGATCACAGATTTTTGGTAGTTTCTGGGTCTACAAGGACTTTCCAAATCCAGGAGCAACGCCAGTGGCAACGTAGTGACTCAGGCGGGCACCAAGGCAACGGCACCATTGGTCTCTGGGTAGTGCTTTAAGAATGAACACAATCACGTTATAGTCCATGGTCCATCACTATTCAAGGATGACTCCCTCCCTTCCTGTCTATTTTTGTTTTTTACTTTTTTACACTGAGTTTCTATTTAGACACTACAACATATGGGGTGTTTGTTCCCATTGGATGCATTTCTATCAAAACTCTATCAAATGTGATGGCTAGATTCTAACATATTGCCATGTGTGGAGTGTGCTGAACACACACCAGTTTACAGGAAAGATGCATTTTGTGTACAGTAAACGGTGTATATACCTTTTGTTACCACAGAGTTTTTTAAACAAATGAGTATTATAGGACTTTCTTCTAAATGAGCTAAATAAGTCACCATTGACTTCTTGGTGCTGTTGAAAATAATCCATTTTCACTAAAAGTGTGTGAAACCTACAGCATATTCTTCACGCAGAGATTTTCATCTATTATACTTTATCAAAGATTGGCCATGTTCCACTTGGAAATGGCATGCAAAAGCCATCATAGAGAAACCTGCGTAACTCCATCTGACAAATTCAAAAGAGAGAGAGAGATCTTGAGAGAGAAATGCTGTTCGTTCAAAAGTGGAGTTGTTTTAACAGATGCCAATTACGGTGTACAGTTTAACAGAGTTTTCTGTTGCATTAGGATAAACATTAATTGGAGTGCAGCTAACATGAGTATCATCAGACTAGTATCAAGTGTTCTAAAATGAAATATGAGAAGATCCTGTCACAATTCTTAGATCTGGTGTCCAGCATGGATGAAACCTTTGAGTTTGGTCCCTAAATTTGCATGAAAGCACAAGGTAAATATTCATTTGCTTCAGGAGTTTCATGTTGGATCTGTCATTATCAAAAGTGATCAGCAATGAAGAACTGGTCGGACAAAATTTAACGTTGATGTAATGGAATTCCAGATGTAGGCATTCCCCCCAGGTCTTTTCATGTGCAGATTGCAGTTCTGATTCATTTGAATAAAAAGGAACTTGGNOV26b, CG55117-01SEQ ID NO: 606865 aaMW at 97200.8kDProtein SequenceMALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPATNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKPETVILGLKIVYYEAGIILCCVLGLLFIILMPLVGYFFCNCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKNKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRNAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMMENGNNGYHKDHVYGIHNPVMTSPSQHNOV26c, CG55117-02SEQ ID NO: 607756 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGCCCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCCACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGGACAGATTTGGATGGCCTGGTCCAACAGGGCTATCAATCCCTTAATGATATACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCAAAAGGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTTCCTATTCAGGATATACTCTCAGCATTCTCTGTTTATGTTAATAACACTGAAAGTTACATCCACAGAAATTTACCTACATTGGAAGAGTATGATTCATACNOV26c, CG55117-02SEQ ID NO: 608252 aaMW at 28267.5kDProtein SequenceQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYNOV26d, CG55117-03SEQ ID NO: 609765 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTGTGAACCTTACACGAGCAAGGAATTATTCCGGGTTTTGGATACACCCTACTTACTAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCTATTTAATAAATCAAAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAGAGGCACTTACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAATATCTTTCTGTTGGGTGCAGCAGGAAGAAAAAACCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGAATTATGACAGCTACTTGGCTCAGACTGGTAAATCCCCCGCAGGAGTGAATCTTTTATCATTTGCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCCCAGGAAATTTGAGGAACTCCCTGAAAAGAGATGCACAAACTATTAAAACAATTCACCAGCAACGAGTCCTTCCTATAGAACAATCACTGAGCACTCTATACCAAAGCGTCAAGATACTTCAACGCACAGGGAATGGATTGTTGGAGAGAGTAACTAGGATTCTAGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCTCTGTTATTATTGAGGAAACTAAGAAGTATGGGAGAACAATAATAGGATATTTTGAACATTATCTGCAGTGGATCGAGTTCTCTATCAGTGAGAAAGTGGCATCGTGCAAACCTNOV26d, CG55117-03SEQ ID NO: 610255 aaMW at 28999.6kDProtein SequenceCEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPNOV26e, CG55117-05SEQ ID NO: 6112523 bpDNA SequenceORF Start: at 7ORF Stop: at 2518GGATCCGGAGGGCAGCCTTCATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCATAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGATTTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGATTGTCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCTGCTGTTTATTATTCTGATGCCTCTGGTGGGGTATTTCTTTTGTATGTGTCGTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAAAATGGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTATAATAATAAGCATTGGCATCTTCTATGGTTTTGTGGCAAATCACCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGCCCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCCACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGGACAGATTTGGATGGCCTGGTCCAACAGGGCTATCAATCCCTTAATGATATACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCAAAAGGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTTCCTATTCAGGATATACTCTCAGCATTCTCTGTTTATGTTAATAACACTGAAAGTTACATCCACAGAAATTTACCTACATTGGAAGAGTATGATTCATACTGGTGGCTGGGTGGCCTGGTCATCTGCTCTCTGCTGACCCTCATCGTGATTTTTTACTACCTGGGCTTACTGTGTGGCGTGTGCGGCTATGACAGGCATGCCACCCCGACCACCCGAGGCTGTGTCTCCAACACCGGAGGCGTCTTCCTCATGGTTGGAGTTGGATTAAGTTTCCTCTTTTGCTGGATATTGATGATCATTGTGGTTCTTACCTTTGTCTTTGGTGCAAATGTGGAAAAACTGATCTGTGAACCTTACACGAGCAAGGAATTATTCCGGGTTTTGGATACACCCTACTTACTAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCTATTTAATAAATCAAAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAGAGGCACTTACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAACATCTCAACATTAATGAGCATACTGGAAGCATAAGCAGTGAATTGGAAAGTCTGAAGGTAAATCTTAATATCTTTCTGTTGGGTGCAGCAGGAAGAAAAAACCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGGATTATGACAGCTACTTGGCTCAGACTGGTAAATCCCCCGCAGGAGTGAATCTTTTATCATTTGCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCCCAGGAAATTTGAGGAACTCCCTGAAAAGAGATGCACAAACTATTAAAACAATTCACCAGCAACGAGTCCTTCCTATAGAACAATCACTGAGCACTCTATACCAAAGCGTCAAGATACTTCAACGCACAGGGAATGGATTGTTGGAGAGAGTAACTAGGACTCTAGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCTCTGTTATTATTGAGGAAACTAAGAAGTATGGGAGGACAATAATAGGATATTTTGAACATTATCTGCAGTGGATCGAGTTCTCTATCAGTGAGAAAGTGGCATCGTGCAAACCTGTGGCCACCGCTCTAGATACTGCTGTTGATGTCTTTCTGTGTAGCTACATTATCGACCCCTTGAATTTGTTTTGGTTTGGCATAGGAAAAGCTACTGTATTTTTACTTCCGGCTCTAATTTTTGCGGTAAAACTGGCTAAGTACTATCGTCGAATGGATTCGGAGGACGTGTACGATGATGTTGAAACTATACCCATGAAAAATATGGAAAATGGTAATAATGGTTATCATAAAGATCATGTATATGGTATTCACAATCCTGTTATGACAAGCCCATCACAACATCTCGAGNOV26e, CG55117-05SEQ ID NO: 612837 aaMW at 94348.3kDProtein SequenceGGQPSSTDAPKAWNYELPATNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKIVYYEAGIILCCVLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMDYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRTLASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQHNOV26f, SNP13382440 ofSEQ ID NO: 6133766 bpCG55117-04, DNA SequenceORF Start: ATG at 10ORF Stop: TGA at 2605SNP Pos: 3274SNP Change: C to ATTGCTAGCTATGGCCCTCGTACTCGGCTCCCTGTTGCTGCTGGGGCTGTGCGGGAACTCCTTTTCAGGAGGGCAGCCTTCATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCATAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGATTTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGCCAGAAACTGTAATCTTAGGTCTAAAGATTGTCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCTGCTGTTTATTATTCTGATGCCTCTGGTGGGGTATTTCTTTTGTATGTGTCGTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAAAATGGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTATAATAATAAGCATTGGCATCTTCTATGGTTTTGTGGCAAATCACCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCAAATCAAATATATATTGGCCCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCCACCCGTGGATGCAGAACTTGACAACGTTAATAACGTTCTTAGGACAGATTTGGATGGCCTGGTCCAACAGGGCTATCAATCCCTTAATGATATACCTGACAGAGTACAACGCCAAACCACGACTGTCGTAGCAGGTATCAAAAGGGTCTTGAATTCCATTGGTTCAGATATCGACAATGTAACTCAGCGTCTTCCTATTCAGGATATACTCTCAGCATTCTCTGTTTATGTTAATAACACTGAAAGTTACATCCACAGAAATTTACCTACATTGGAAGAGTATGATTCATACTGGTGGCTGGGTGGCCTGGTCATCTGCTCTCTGCTGACCCTCATCGTGATTTTTTACTACCTGGGCTTACTGTGTGGCGTGTGCGGCTATGACAGGCATGCCACCCCGACCACCCGAGGCTGTGTCTCCAACACCGGAGGCGTCTTCCTCATGGTTGGAGTTGGATTAAGTTTCCTCTTTTGCTGGATATTGATGATCATTGTGGTTCTTACCTTTGTCTTTGGTGCAAATGTGGAAAAACTGATCTGTGAACCTTACACGAGCAAGGAATTATTCCGGGTTTTGGATACACCCTACTTACTAAATGAAGACTGGGAATACTATCTCTCTGGGAAGCTATTTAATAAATCAAAAATGAAGCTCACTTTTGAACAAGTTTACAGTGACTGCAAAAAAAATAGAGGCACTTACGGCACTCTTCACCTGCAGAACAGCTTCAATATCAGTGAACATCTCAACATTAATGAGCATACTGGAAGCATAAGCAGTGAATTGGAAAGTCTGAAGGTAAATCTTAATATCTTTCTGTTGGGTGCAGCAGGAAGAAAAAACCTTCAGGATTTTGCTGCTTGTGGAATAGACAGAATGAATTATGACAGCTACTTGGCTCAGACTGGTAAATCCCCCGCAGGAGTGAATCTTTTATCATTTGCATATGATCTAGAAGCAAAAGCAAACAGTTTGCCCCCAGGAAATTTGAGGAACTCCCTGAAAAGAGATGCACAAACTATTAAAACAATTCACCAGCAACGAGTCCTTCCTATAGAACAATCACTGAGCACTCTATACCAAAGCGTCAAGATACTTCAACGCACAGGGAATGGATTGTTGGAGAGAGTAACTAGGATTCTAGCTTCTCTGGATTTTGCTCAGAACTTCATCACAAACAATACTTCCTCTGTTATTATTGAGGAAACTAAGAAGTATGGGAGAACAATAATAGGATATTTTGAACATTATCTGCAGTGGATCGAGTTCTCTATCAGTGAGAAAGTGGCATCGTGCAAACCTGTGGCCACCGCTCTAGATACTGCTGTTGATGTCTTTCTGTGTAGCTACATTATCGACCCCTTGAATTTGTTTTGGTTTGGCATAGGAAAAGCTACTGTATTTTTACTTCCGGCTCTAATTTTTGCGGTAAAACTGGCTAAGTACTATCGTCGAATGGATTCGGAGGACGTGTACGATGATGTTGAAACTATACCCATGAAAAATATGGAAAATGGTAATAATGGTTATCATAAAGATCATGTATATGGTATTCACAATCCTGTTATGACAAGCCCATCACAACATTGATAGCTGATGTTGAAACTGCTTGAGCATCAGCATACTCAAAGTGGAAAGGATCACAGATTTTTGGTAGTTTCTGGGTCTACAAGGACTTTCCAAATCCAGGAGCAACGCCAGTGGCAACGTAGTGACTCAGGCGGGCACCAAGGCAACGGCACCATTGGTCTCTGGGTAGTGCTTTAAGAATGAACACAATCACGTTATAGTCCATGGTCCATCACTATTCAAGGATGACTCCCTCCCTTCCTGTCTATTTTTGTTTTTTACTTTTTTACACTGAGTTTCTATTTAGACACTACAACATATGGGGTGTTTGTTCCCATTGGATGCATTTCTATCAAAACTCTATCAAATGTGATGGCTAGATTCTAACATATTGCCATGTGTGGAGTGTGCTGAACACACACCAGTTTACAGGAAAGATGCATTTTGTGTACAGTAAACGGTGTATATACCTTTTGTTACCACAGAGTTTTTTAAACAAATGAGTATTATAGGACTTTCTTCTAAATGAGCTAAATAAGTCACCATTGACTTCTTGGTGCTGTTGAAAATAATCCATTTTCACTAAAAGTGTGTGAAACCTACAGCATATTCTTCACGCAGAGATTTTCATCTATTATACTTTATCAAAGATTGGCCATGTTCCACTTGGAAATGGCATGCAAAAGCAATCATAGAGAAACCTGCGTAACTCCATCTGACAAATTCAAAAGAGAGAGAGAGATCTTGAGAGAGAAATGCTGTTCGTTCAAAAGTGGAGTTGTTTTAACAGATGCCAATTACGGTGTACAGTTTAACAGAGTTTTCTGTTGCATTAGGATAAACATTAATTGGAGTGCAGCTAACATGAGTATCATCAGACTAGTATCAAGTGTTCTAAAATGALATATGAGAAGATCCTGTCACAATTCTTAGATCTGGTGTCCAGCATGGATGAAACCTTTGAGTTTGGTCCCTAAATTTGCATGAAAGCACAAGGTAAATATTCATTTGCTTCAGGAGTTTCATGTTGGATCTGTCATTATCAAAAGTGATCAGCAATGAAGAACTGGTCGGACAAAATTTAACGTTGATGTAATGGAATTCCAGATGTAGGCATTCCCCCCAGGTCTTTTCATGTGCAGATTGCAGTTCTGATTCATTTGAATAAAAAGGAACTTGGNOV26f, SNP13382440 ofSEQ ID NO: 614865 aaMW at 97200.8kDCG55117-04, ProteinSNP Change: no changeSequenceMALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPATNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRXFLQKAYESKIDYDKPETVILGLKIVYYEAGIILCCVLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQHNOV26g, SNP13382439 ofSEQ ID NO: 6153766 bpCG55117-04, DNA SequenceORF Start: ATG at 10ORF Stop: TGA at 2605SNP Pos: 3685SNP Change: G to ATTGCTAGCTATGGCCCTCGTACTCGGCTCCCTGTTGCTGCTGGGGCTGTGCGGGAACTCCTTTTCAGGAGGGCAGCCTTCATCCACAGATGCTCCTAAGGCTTGGAATTATGAATTGCCTGCAACAAATTATGAGACCCAAGACTCCCATAAAGCTGGACCCATTGGCATTCTCTTTGAACTAGTGCATATCTTTCTCTATGTGGTACAGCCGCGTGATTTCCCAGAAGATACTTTGAGAAAATTCTTACAGAAGGCATATGAATCCAAAATTGATTATGACAAGCCAGAAACTGTAATCTTAGGTCTAAAGATTGTCTACTATGAAGCAGGGATTATTCTATGCTGTGTCCTGGGGCTGCTGTTTATTATTCTGATGCCTCTGGTGGGGTATTTCTTTTGTATGTGTCGTTGCTGTAACAAATGTGGTGGAGAAATGCACCAGCGACAGAAGGAAAATGGGCCCTTCCTGAGGAAATGCTTTGCAATCTCCCTGTTGGTGATTTGTATAATAATAAGCATTGGCATCTTCTATGGTTTTGTGGCAAATCACCAGGTAAGAACCCGGATCAAAAGGAGTCGGAAACTGGCAGATAGCAATTTCAAGGACTTGCGAACTCTCTTGAATGAAACTCCAGAGCPAATCAAATATATATTGGCCCAGTACAACACTACCAAGGACAAGGCGTTCACAGATCTGAACAGTATCAATTCAGTGCTAGGAGGCGGAATTCTTGACCGACTGAGACCCAACATCATCCCTGTTCTTGATGAGATTAAGTCCATGGCAACAGCGATCAAGGAGACCAAAGAGGCGTTGGAGAACATGAACAGCACCTTGAAGAGCTTGCACCAACAAAGTACACAGCTTAGCAGCAGTCTGACCAGCGTGAAAACTAGCCTGCGGTCATCTCTCAATGACCCTCTGTGCTTGGTGCATCCATCAAGTGAAACCTGCAACAGCATCAGATTGTCTCTAAGCCAGCTGAATAGCAACCCTGAACTGAGGCAGCTTCCACCC


Further analysis of the NOV26a protein yielded the following properties shown in Table 26C.

TABLE 26CProtein Sequence Properties NOV26aSignalP analysis:Cleavage site between residues 20 and 21PSORTII analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 26; peak value 9.51PSG score:5.11GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 2.78possible cleavage site: between 19 and 20>>> Seems to have a cleavable signal peptide (1 to 19)ALOM:Klein et al's method for TM region allocationInit position for calculation: 20Tentative number of TMS(s) for the threshold 0.5: 6INTEGRALLikelihood =Transmembrane  50-66 −3.08INTEGRALLikelihood =Transmembrane 109-125−13.69INTEGRALLikelihood =Transmembrane 158-174−12.90INTEGRALLikelihood =Transmembrane 435-451 −8.55INTEGRALLikelihood =Transmembrane 491-507−13.06INTEGRALLikelihood =Transmembrane 797-813 −3.13PERIPHERALLikelihood = 0.90 (at 772)ALOM score: −13.69 (number of TMSs: 6)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 9Charge difference: −2.0 C(−1.0)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):0.43Hyd Moment(95):2.59G content:5D/E content:1S/T content:6Score: −6.77Gavel:Prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 9.5%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs:Leucine zipper pattern (PS00029): *** found *** LPPVDAELDNVNNVLRTDLDGL at 335nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regions251 V0.95252 L0.99253 D0.99254 E0.99255 I0.99256 K0.99257 S0.99258 M0.99259 A0.99260 T0.99261 A0.99262 I0.99263 K0.99264 E0.99265 T0.99266 K0.99267 E0.99268 A0.99269 L0.99270 E0.99271 N0.99272 M0.99273 N0.99274 S0.99275 T0.99276 L0.99277 K0.99278 S0.99279 L0.99280 H0.98281 Q0.98282 Q0.97283 S0.90284 T0.90285 Q0.90286 L0.90287 S0.90288 S0.90289 S0.89290 L0.89291 T0.67292 S0.51293 V0.51294 K0.51total: 44 residuesFinal Results (k = 9/23):77.8%: endoplasmic reticulum22.2%: mitochondrial>> prediction for CG55117-04 is end (k = 9)


A search of the NOV26a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 26D.

TABLE 26DGeneseq Results for NOV26aNOV26aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABJ37079Human breast cancer/ovarian cancer1 . . . 865865/865 (100%)0.0related protein #55 - Homo sapiens,1 . . . 865865/865 (100%)865 aa. [WO2003000012-A2, 03-JAN-2003]ABB82955Human PROML related protein1 . . . 865865/865 (100%)0.0(GenBank Identifier No. GI#131214442) -1 . . . 865865/865 (100%)Homo sapiens, 865 aa.[WO200299047-A2, 12-DEC-2002]ABB82954Human PROML related protein1 . . . 865865/865 (100%)0.0(GenBank Identifier No. GI#5174387) -1 . . . 865865/865 (100%)Homo sapiens, 865 aa.[WO200299047-A2, 12-DEC-2002]ABB82953Human PROML related protein1 . . . 865865/865 (100%)0.0(GenBank Identifier No. GI#7512271) -1 . . . 865865/865 (100%)Homo sapiens, 865 aa.[WO200299047-A2, 12-DEC-2002]AAU79943Human AC133 antigen-like protein1 . . . 865865/865 (100%)0.0NOV5 - Homo sapiens, 865 aa.1 . . . 865865/865 (100%)[WO200229038-A2, 11-APR-2002]


In a BLAST search of public sequence databases, the NOV26a protein was found to have homology to the proteins shown in the BLASTP data in Table 26E.

TABLE 26EPublic BLASTP Results for NOV26aNOV26aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueO43490Prominin-like protein 1 precursor1 . . . 865865/865(100%)0.0(Antigen AC133) (CD133 antigen) -1 . . . 865865/865(100%)Homo sapiens (Human), 865 aa.Q96EN6Similar to prominin (Mouse)-like 11 . . . 865856/865(98%)0.0(AC133-2 antigen) (MSTP061) -1 . . . 856856/865(98%)Homo sapiens (Human), 856 aa.Q91XN5Prominin - Rattus norvegicus (Rat),1 . . . 865528/866(60%)0.0857 aa.1 . . . 857681/866(77%)O54990Prominin-like protein 1 precursor1 . . . 865526/867(60%)0.0(Antigen AC133 homolog) - Mus 1 . . . 867683/867(78%)musculus (Mouse), 867 aa.T08881prominin - mouse, 858 aa.1 . . . 865521/867(60%)0.01 . . . 858676/867(77%)


PFam analysis predicts that the NOV26a protein contains the domains shown in the Table 26F.

TABLE 26FDomain Analysis of NOV26aPfamNOV26aIdentities/ExpectDomainMatch RegionSimilarities forValuethe Matched Region


Example 27

The MOV27 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 27A.

TABLE 27ANOV27 Sequence AnalysisNOV27a, CG55193-04SEQ ID NO: 6171336 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1327ATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTAATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAAGGTCAGTGCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCTGAGGAGGCTNOV27a, CG55193-04SEQ ID NO: 618442 aaMW at 50568.7kDProtein SequenceMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLIQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTPAKDAIMQMWLNARPDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGNOV27b, 214575880SEQ ID NO: 6191188 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCACCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTCATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGTCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAGGAGCTGGCCCGGAGCCTCCGGGCGGATATCGAACGCGTGGTCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAGGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCCTCGAGNOV27b, 214575880SEQ ID NO: 620396 aaMW at 45387.5kDProtein SequenceGSNVHVSTESNLQATEHRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLVKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVVRENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRRERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGLENOV27c, 214458684SEQ ID NO: 6211419 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGAGATTCCCCCCCCTCGGGTCTCTTGGGCCTGTCCATGCGCCCCCCCCTCCGTGCTCCATGGCCAGACCCATGGATCCATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTCATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAGGAGCTGGCCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCCTCGAGNOV27c, 214458684SEQ ID NO: 622470 aaMW at 53542.0kDProtein SequenceEIPPPRVSWACPCAPPSVLHGQTHGSMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLIQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGLENOV27d, 214458688SEQ ID NO: 6231404 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTTCGCCCTTGGATCCATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTCATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCGGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAGGAGCTGGCCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCCTCGAGGCCACTGGATGGGGCTACAGGGATGCCTCCGTGCTCCATGGCCAGACCCATGGATCCNOV27d, 214458688SEQ ID NO: 624468 aaMW at 53321.6kDProtein SequenceFALGSMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLIQFLITLGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELRHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGLEATGWGYPDASVLHGQTHGSNOV27e, CG55193-01SEQ ID NO: 6251366 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1327ATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTAATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAAGGTCAGTGCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCTGAGGAGGCTCCGGCACTGACCTAAGGGCGAATCCCAGGANOV27e, CG55193-01SEQ ID NO: 626442 aaMW at 50568.7kDProtein SequenceMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLIQFLIILGLVLFMVYGNVHVSTESNLQATERPAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLHASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGNOV27f, CG55193-02SEQ ID NO: 6271367 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1327ATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTAATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAAGGTCAGTGCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCTGAGGAGGCTCCGGCACTGACCTAAGGGCGAATCCCAGCAANOV27f, CG55193-02SEQ ID NO: 628442 aaMW at 50568.7kDProtein SequenceMGLAMEHGGSYAPAGGSSRGCWYYLRYFFLFVSLIQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGNOV27g, CG55193-03SEQ ID NO: 6291367 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1327ATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTAATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAAGGTCAGTGCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCTGAGGAGGCTCCGGCACTGACCTAAGGGCGAATCCCAGCAANOV27g, CG55193-03SEQ ID NO: 630442 aaMW at 50568.7kDProtein SequenceMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLIQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGNOV27h, CG55193-05SEQ ID NO: 6311188 bpDNA SequenceORF Start: at 7ORF Stop: at 1183GGATCCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCACCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTCATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACALACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGTCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAGGAGCTGGCCCGGAGCCTCCGGGCGGATATCGAACGCGTGGTCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAGGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCCTCGAGNOV27h, CG55193-05SEQ ID NO: 632392 aaMW at 45001.1kDProtein SequenceNVHVSTESNLQATEHRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLVKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVVRENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRRERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGNOV27i, CG55193-06SEQ ID NO: 6331188 bpDNA SequenceORF Start: at 7ORF Stop: at 1183GGATCCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCCGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTCATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAGGAGCTGGCCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCCTCGAGNOV27i, CG55193-06SEQ ID NO: 634392 aaMW at 44936.0kDProtein SequenceNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTEAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMNPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGNOV27j, CG55193-07SEQ ID NO: 6351410 bpDNA SequenceORF Start: ATG at 79ORF Stop: at 1405GAGATTCCCCCCCCTCGGGTCTCTTGGGCCTGTCCATGCGCCCCCCCCTCCGTGCTCCATGGCCAGACCCATGGATCCATGGGTCTGGCCATGGAGCACGGAGGGTCCTACGCTCGGGCGGGGGGCAGCTCTCGGGGCTGCTGGTATTACCTGCGCTACTTCTTCCTCTTCGTCTCCCTCATCCAATTCCTCATCATCCTGGGGCTCGTGCTCTTCATGGTCTATGGCAACGTGCACGTGAGCACAGAGTCCAACCTGCAGGCCACCGAGCGCCGAGCCGAGGGCCTATACAGTCAGCTCCTAGGGCTCACGGCCTCCCAGTCCAACTTGACCAAGGAGCTCAACTTCACCACCCGCGCCAAGGATGCCATCATGCAGATGTGGCTGAATGCTCGCcGCGACCTGGACCGCATCAATGCCAGCTTCCGCCAGTGCCAGGGTGACCGGGTCATCTACACGAACAATCAGAGGTACATGGCTGCCATCATCTTGAGTGAGAAGCAATGCAGAGATCAATTCAAGGACATGAACAAGAGCTGCGATGCCTTGCTCTTCATGCTGAATCAGAAGGTGAAGACGCTGGAGGTGGAGATAGCCAAGGAGAAGACCATTTGCACTAAGGATAAGGAAAGCGTGCTGCTGAACAAACGCGTGGCGGAGGAACAGCTGGTTGAATGCGTGAAAACCCGGGAGCTGCAGCACCAAGAGCGCCAGCTGGCCAAGGAGCAACTGCAAAAGGTGCAAGCCCTCTGCCTGCCCCTGGACAAGGACAAGTTTGAGATGGACCTTCGTAACCTGTGGAGGGACTCCATTATCCCACGCAGCCTGGACAACCTGGGTTACAACCTCTACCATCCCCTGGGCTCGGAATTGGCCTCCATCCGCAGAGCCTGCGACCACATGCCCAGCCTCATGAGCTCCAAGGTGGAGGAGCTGGCCCGGAGCCTCCGGGCGGATATCGAACGCGTGGCCCGCGAGAACTCAGACCTCCAACGCCAGAAGCTGGAAGCCCAGCAGGGCCTGCGGGCCAGTCAGGAGGCGAAACAGAAGGTGGAGAAGGAGGCTCAGGCCCGGGAGGCCAAGCTCCAAGCTGAATGCTCCCGGCAGACCCAGCTAGCGCTGGAGGAGAAGGCGGTGCTGCGGAAGGAACGAGACAACCTGGCCAAGGAGCTGGAAGAGAAGAAGAGGGAGGCGGAGCAGCTCAGGATGGAGCTGGCCATCAGAAACTCAGCCCTGGACACCTGCATCAAGACCAAGTCGCAGCCGATGATGCCAGTGTCAAGGCCCATGGGCCCTGTCCCCAACCCCCAGCCCATCGACCCAGCTAGCCTGGAGGAGTTCAAGAGGAAGATCCTGGAGTCCCAGAGGCCCCCTGCAGGCATCCCTGTAGCCCCATCCAGTGGCCTCGAGNOV27j, CG55193-07SEQ ID NO: 636442 aaMW at 50593.7kDProtein SequenceMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLIQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESvLLNKRvAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKTLESQRPPAGIPVAPSSG


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 27B.

TABLE 27BComparison of the NOV27 protein sequences.NOV27a --------------------------MGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLNOV27b ------------------------------------------------------------NOV27c EIPPPRVSWACPCAPPSVLHGQTHGSMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLNOV27d ---------------------FALGSMGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLNOV27e --------------------------MGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLNOV27f --------------------------MGLAMEHGGSYAHAGGSSRGCWYYLRYFFLFVSLNOV27g --------------------------MGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLNOV27h ------------------------------------------------------------NOV27i ------------------------------------------------------------NOV27j --------------------------MGLAMEHGGSYARAGGSSRGCWYYLRYFFLFVSLNOV27a IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27b --------------GSNVHVSTESNLQATEHRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27c IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27d IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27e IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27f IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27g IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27h ----------------NVHVSTESNLQATEHRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27i ----------------NVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27j IQFLIILGLVLFMVYGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRANOV27a KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27b KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27c KDAIMQMWLNAREDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27d KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27e KDAIMQMWLNARPDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27f KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27g KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27h KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYNAAIILSEKQCRDQFKDMNKSCNOV27i KDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27j KDAIMQMWLNARPDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCNOV27a DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27b DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLVNOV27c DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27d DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELRHQERQLANOV27e DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27f DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27g DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27h DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLVNOV27i DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27j DALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLANOV27a KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHNOV27b KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHNOV27c KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHNOV27d KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHNOV27e KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRPACDHNOV27f KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHNOV27g KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRPACDHNOV27h KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRPACDHNOV27i KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRPACDHNOV27j KEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHNOV27a MPSLMSSKVEGQCRSLRADIERVAPENSDLQRQKLEAQQGLEASQEAKQKVEKEAQAREANOV27b MPSLMSSKVEELARSLRADIERVVRENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREANOV27c MPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREANOV27d MPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLEASQEAKQKVEKEAQAREANOV27e MPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLEASQEAKQKVEKEAQAREANOV27f MPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLPASQEAKQKVEKEAQAREANOV27g MPSLMSSKVEGQCRSLRADIERVARENSDLQRQKLEAQQGLPASQEAKQKVEKEAQAREANOV27h MPSLMSSKVEELARSLHADIERVVRENSDLQRQKLEAQQGLEASQEAKQKVEKEAQAREANOV27i MPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREANOV27j MPSLMSSKVEELARSLEADIERVARENSDLQRQKLEAQQGLPASQEAKQKVEKEAQAREANOV27a KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMETAIRNSALDTCIKTKSNOV27b KLQAECSRQTQLALEEKAVLRRERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27c KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27d KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27e KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLPMELAIRNSALDTCIKTKSNOV27f KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27g KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRNELAIRNSALDTCIKTKSNOV27h KLQAECSRQTQLALEEKAVLRRERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27i KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27j KLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSNOV27a QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27b QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGLE----------NOV27c QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGLE----------NOV27d QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSGLEATGWGYRDASNOV27e QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27f QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27g QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27h QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27i QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27j QPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG------------NOV27a ---------NOV27b ---------NOV27c ---------NOV27d VLHGQTHGSNOV27e ---------NOV27f ---------NOV27g ---------NOV27h ---------NOV27i ---------NOV27j ---------NOV27a (SEQ ID NO: 618)NOV27b (SEQ ID NO: 620)NOV27c (SEQ ID NO: 622)NOV27d (SEQ ID NO: 624)NOV27e (SEQ ID NO: 626)NOV27f (SEQ ID NO: 628)NOV27g (SEQ ID NO: 630)NOV27h (SEQ ID NO: 632)NOV27i (SEQ ID NO: 634)NOV27j (SEQ ID NO: 636)


Further analysis of the NOV27a protein yielded the following properties shown in Table 27C.

TABLE 27CProtein Sequence Properties NOV27aSignalP analysis:Cleavage site between residues 51 and 52PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos. chg 0; neg. chg 1H-region:length 6; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −1.10possible cleavage site: between 50 and 51>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 29-25−11.41PERIPHERALLikelihood = 7.85 (at 156)ALOM score: −11.41 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 36Charge difference: −2.5 C(0.5)-N(3.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 29)MITDISC:discrimination of mitochondrial targeting seqR content:3Hyd Moment(75):5.06Hyd Moment (95):6.63G content:8D/E content:2S/T content:6Score: −6.39Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 36 LRY|FFNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: RKERDNLAKELEEKKRE at 355content of basic residues: 15.6%NLS Score: 0.02KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: 10, 22, 23, 26Dileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regions278 M0.60279 S0.85280 S0.96281 K0.96282 V0.96283 E0.98284 G0.98285 Q0.99286 C0.99287 R0.99288 S0.99289 L0.99290 R0.99291 A0.99292 D0.99293 I0.99294 E0.99295 R0.99296 V0.99297 A0.99298 R0.99299 E0.99300 N0.99301 S0.99302 D1.00303 L1.00304 Q1.00305 R1.00306 Q1.00307 K1.00308 L1.00309 E1.00310 A1.00311 Q1.00312 Q1.00313 G1.00314 L1.00315 R1.00316 A1.00317 S1.00318 Q1.00319 E1.00320 A1.00321 K1.00322 Q1.00323 K1.00324 V1.00325 E1.00326 K1.00327 E1.00328 A1.00329 Q1.00330 A0.99331 R0.96332 E0.96333 A0.96334 K0.96335 L0.96336 Q0.96337 A0.87338 E0.72339 C0.71340 S0.75341 R0.92342 Q0.92343 T0.93344 Q0.99345 L1.00346 A1.00347 L1.00348 E1.00349 E1.00350 K1.00351 A1.00352 V1.00353 L1.00354 R1.00355 K1.00356 E1.00357 R1.00358 D1.00359 N1.00360 L1.00361 A1.00362 K1.00363 E1.00364 L1.00365 E1.00366 E1.00367 K1.00368 K1.00369 R1.00370 E1.00371 A1.00372 E1.00373 Q1.00374 L1.00375 R1.00376 M1.00377 E1.00378 L1.00379 A1.00380 I1.00381 R0.97382 N0.97383 S0.97384 A0.97385 L0.97386 D0.97387 T0.91388 C0.64389 I0.64390 K0.64total: 113 residuesFinal Results (k = 9/23):39.1%: nuclear26.1%: mitochondrial17.4%: cytoplasmic 4.3%: extracellular, including cell wall 4.3%: Golgi 4.3%: plasma membrane 4.3%: peroxisomal>> prediction for CG55193-04 is nuc (k = 23)


A search of the NOV27a protein against the Geneseq database, a proprietary se that contains sequences published in patents and patent publication, yielded homologous proteins shown in Table 27D.

TABLE 27DGeneseq Results for NOV27aNOV27aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG78367Human protein, homologous to PV-11 . . . 442442/442 (100%) 0.0receptor, designated NOV7a - Homo1 . . . 442442/442 (100%) sapiens, 442 aa. [WO200226826-A2,04-APR-2002]AAE06600Human protein having hydrophobic1 . . . 442439/442 (99%)0.0domain, HP10787 - Homo sapiens, 442 aa.1 . . . 442439/442 (99%)[WO200149728-A2, 12-JUL-2001]AAM41619Human polypeptide SEQ ID NO 6550 -1 . . . 442439/442 (99%)0.0Homo sapiens, 457 aa.16 . . . 457 439/442 (99%)[WO200153312-A1, 26-JUL-2001]AAB42154Human ORFX ORF1918 polypeptide sequence1 . . . 442439/442 (99%)0.0SEQ ID NO: 3836 - Homo38 . . . 479 439/442 (99%)sapiens, 479 aa. [WO200058473-A2,05-OCT-2000]ABG78369Human protein, homologous to PV-11 . . . 442436/442 (98%)0.0receptor, designated NOV7c - Homo1 . . . 442439/442 (98%)sapiens, 442 aa. [WO200226826-A2,04-APR-2002]


In a BLAST search of public sequence databases, the NOV27a protein was found to have homology to the proteins shown in the BLASTP data in Table 27E.

TABLE 27EPublic BLASTP Results for NOV27aNOV27aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueAAH50365PLVAP protein - Homo sapiens1 . . . 442439/442 (99%)0.0(Human), 455 aa (fragment).14 . . . 455 439/442 (99%)Q9BX97PV1 protein - Homo sapiens1 . . . 442439/442 (99%)0.0(Human), 442 aa.1 . . . 442439/442 (99%)Q8ND68Hypothetical protein - Homo sapiens1 . . . 442439/442 (99%)0.0(Human), 456 aa (fragment).15 . . . 456 439/442 (99%)Q9BZD5Fenestrated-endothelial linked1 . . . 442438/442 (99%)0.0structure protein - Homo sapiens1 . . . 442438/442 (99%)(Human), 442 aa.Q8N8Y0Hypothetical protein FLJ38711 -1 . . . 442433/442 (97%)0.0Homo sapiens (Human), 437 aa.1 . . . 437433/442 (97%)


PFam analysis predicts that the NOV27a protein contains the domains shown in the Table 27F.

TABLE 27FDomain Analysis of NOV27aPfamNOV27aIdentities/ExpectDomainMatch RegionSimilaritiesValuefor theMatched Region


Example 28

The NOV28 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 28A.

TABLE 28ANOV28 Sequence AnalysisNOV28a, CG55256-07SEQ ID NO:6374294 bpDNA SequenceORF Start: ATG at 103ORF Stop: TGA at 2818GCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGCGGCGGCGGCGGCGCGGGGAGCGCGGGGCGCGGCGGCCGGAGCCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGAGTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGAGTCACCCTGTGAGGGTCCCCGCAGATTTCAGTGTAAGAGTGGCAAGCGCGTGGACGGCGGGAAAGTGTGTGATGTGCAGAGGGACTGCCGGGACTGGTCGGATGAGCTTCTGAAAGTGTGGTGCGGTGCCTGTCTACGCCCACTGGCTGGACTCAGTCTCCTACCATCCCCCTCCTGGTATCTAGGCTCAAGGCCCTCCAGTGCCCCCTGCCCTGACACTTTCTGCTCTGACCCTCTCTTTGGATTCATGTGCCGTCCTATGGCTTCACATGGGGCTTTTCGCCCCCAGGCCTCTGGTCTACATCTCTACAAAGTGCTAAGAGCTTGTCCATCCCAGAGGAGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCCAAGCTGCTGCTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGTACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAACAAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGATGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATGGAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAAGATGTTTGGTTGCTGGGGGGGCTTTTTTGGGTTTTGGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTAAAAAAAAAAAAAAAACACTTCAACTCCCTCCCCCATTTAGATTATTTATTAACATATTTTAAAAATCAGATGAGTTCTATAAATAATTTAGAGAAGTGAGAGTATTTATTTTTGGCATGTTTGGCCCACCACACAGACTCTGTGTGTGTATGTGTGTGTTTATATGTGTATGTGTGTGACAGGAAAATCTGTAGAGAAGAGGCACATCTATGGCTACTGTTCAAATACATAAAGATAAATTTATTTTCACACAGTCCACAAGGGCTATATCTTGTAGTTTTCAGAAAAGCCTTTGGAAATCTGGATCAGGAAATAGATACCATGGTTTGTGCAATTATGTAGTAAAAAGGCAAATCTTTTCACCTCTGGCTATTCCTGAGACCCCAGGAAGTCAGGAAAAAGCCTTTCAGCTCACCCATGGCTGCTGTGACTCCTACCAGGGCTTTCTTGGCTTTGGCGAAGGTCAGTGTACAGACATTCCATGGTACCAGAGTGCTCAGAAAGTCAAGATAGGATATGCCTCACCCTCAGCTACTCCTTGTTTTAAAGTTCAGCTCTTTGAGTAACTTCTTCAATTTCTTTCAGGACACTTGGGTTGAATTCAGTAAGTTTCCTCTGAAGCACCCTGAAGGGTGCCATCCTTACAGAGCTAAGTGGAGACGTTTCCAGATCAGCCCAAGTTTACTATAGAGACTGGCCCAGGCACTGAATGTCTAGGACATGCTGTGGATGAAGATAAAGATGGTGGAATAGGTTTTATCACATCTCTTATTTCTCTTTTCCCCTTACTCTCTACCATTTCCTTTATGTGGGGAAACATTTTAAGGTAATAAATAGGTTACTTACCATCATATGTTCATATAGATGAAACTAATTTTTGGCTTAAGTCAGAACAACTGGCCCCCAATTGAAGTCATATTTGTGGGGGGAAATGGCATACGCAATATTATATTATATTGGATATTTATGTTCACACAGGAATTTGGTTTACTGCTTTGTAAATAAAAGGGAAAACTCCGGGTANOV28a, CG55256-07SEQ ID NO:638905 aaMW at 100461.4 kDProtein SequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQESPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCGACLRPLAGLSLLPSPSWYLGSRPSSAPCPDTFCSDPLFGFMCRPMASHGAFRPQASGLHLYKVLRACPSQRRLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCQAAACKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPPAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPHAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPEAPSISPSTLSPATSINHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRAWKRATKSDAFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28b, CG55256-10SEQ ID NO:6391079 bpDNA SequenceORF Start: at 1ORF Stop: TGA at 1009GCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGACGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGATGGTGTATGANOV28b, CG55256-10SEQ ID NO:640336 aaMW at 36894.7 kDProtein SequenceAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28c, 272511714SEQ ID NO:6411983 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGGGCTGAACGAGTGTCGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGCTGGCAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGATGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGCCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGTTCCCAGCATCAGCCCGTCTACCCTAAGACCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGCTCGAGNOV28c, 272511714SEQ ID NO:642661 aaMW at 73025.5 kDProtein SequenceGSAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATSLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICADLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLMKHNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQPLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRVPSISPSTLRPATSNHSQHYANEDSKLENOV28d, CG55256-01SEQ ID NO:6432537 bpDNA SequenceORF Start: ATG at 30ORF Stop: TGA at 2505TGTTTTTTTTCTTCAGTACTTTAAATGTCATGCCACTCTCCTGGCCTGTAAGCTTTCCACAGAGAAGCCTGCTGCCAGACCCATTGGAGCTTCTTTGTGTGTTATTTGTTTCTTTTTTCTTGCTGCTTTTACAGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCTGCAGCCAAGAAGACACCTGTGAGAAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACTAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGCTGGCAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCTTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTGAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGACGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCAGAAGACCCAGCCCCTGCCCTGAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGNOV28d, CG55256-01SEQ ID NO:6441825 aaMW at 90830.8 kDProtein SequenceMPLSWPVSFPQRSLLPDPLELLCVLFVSFFLLLLQLQHLAAAAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPAAKKTPVRSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADAGCATSLGTCRGDEFQCGDGTCVIAAIAACNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRPRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHANEDSKMGSTVTAAVIGIIVPIANEDSKMGSTVTAAVIGIIVPIGDSPPVHEWIPDLEKLEAEEHQKHEFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKQRVALSLEDDGLPNOV28e, CG55256-02SEQ ID NO:6453039 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 3037ATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTAGTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTTGTGCGCCCCGCACGAGTTCCAGTGCGGCAACCGCTCGTGCCTGGCCGCCGTGTTCGTGTGCGACGGCGATGACGACTGTGGTGACGGCAGCGATGAGCGCGGCTGTGCAGACCCGGCCTGCGGGCCCCGCGAGTTCCGCTGCGGCGGCGATGGCGGCGGCGCCTGCATCCCGGAGCGCTGGGTCTGCGACCGCCAGTTTGACTGCGAGGACCGCTCGGACGAGGCAGCCGAGCTCTGCGGCCGCCCGGGCCCCGGGGCCACGTCCGCGCCCGCCGCCTGCGCCACCGTCTCCCAGTTCGCCTGCCGCAGCGGCGAGTGCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGGTCCCCGCAGATTTCAGTGTAAGAGTGGCAAGCGCGTGGACGGCGGGAAAGTGTGTGATGTGCAGAGGGACTGCCGGGACTGGTCGGATGAGCTTCTGAAAGTGTGGTGCGTCTCCTACCATCCCCCTCCTGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGCTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGANOV28e, CG55256-02SEQ ID NO:6461012 aaMW at 111334.6 kDProtein SequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATLCAPHEFQCGNRSCLAAVFVCDGDDDCGDGSDERGCADPACGPREFRCGGDGGGACIPERWVCDRQFDCEDRSDEAAELCGRPGPGATSAPAACATVSQFACRSGECVHLGWRCDGDRDCKDKSDEADCPLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQESPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCVSYHPPPGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPAAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28f, CG55256-03SEQ ID NO:6472537 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 2086ATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATAGTGCAAGGACCCAGATGOCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGCTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGAACATCTCTGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCACGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCCGGATGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTTTAAATTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAAGATGTTTGGTTGCTGGGGGGCTTTTTTAGGTTTTGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTNOV28f, CG55256-03SEQ ID NO:648695 aaMW at 77399.4 kDProtein SequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATSLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKNISGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRDANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPARVALSLEDDGLPNOV28g, CG55256-04SEQ ID NO:6492474 bpDNA SequenceORF Start: ATG at 30ORF Stop: TGA at 2442TGTTTTTTTTCTTCAGTACTTTAAATGTCATGCCACTCTCCTGGCCTGTAAGCTTTCCACAGAGAAGCCTGCTGCCAGACCCATTGGAGCTTCTTTGTGTGTTATTTGTTTCTTTTTTCTTGCTGCTTTTACAGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCTGCAGCCAAGAAGACACCTGTGAGAAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACTAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGATCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGCTGGCAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCTTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTGAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGACGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGNOV28g, CG55256-04SEQ ID NO:650804 aaMW at 88873.0 kDProtein Sequence1804MPLSWPVSFPQRSLLPDPLELLCVLFVSFFLLLLQLQHLAAAAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPAAKKTPVRSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATSLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTAAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPPAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLP+TL,1NOV28h, CG55256-05SEQ ID NO:6514150 bpDNA SequenceORF Start: ATG at 103ORF Stop: TGA at 2674GCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGCGGCGGCGGCGGCGCGGGGACCGCGGGGCGCGGCGGCCGGAGCCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGAGTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGAGTCACCCTGTGAGGGTCCCCGCAGATTTCAGTGTAAGAGTGGCAAGCGCGTGGACGGCGGGAAAGTGTGTGATGTGCAGAGGGACTGCCGGGACTGGTCGGATGAGCTTCTGAAAGTGTGGTGCGGTGCCTGTCTACGCCCACTGGCTGGACTCAGTCTCCTACCATCCCCCTCCTGGAGCCCATGTCCCTCCCCAGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTAACTGCAAGGCTGCTGCTGGCAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGATGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAAGATGTTTGGTTGCTGGGGGGGCTTTTTTGGGTTTTGGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTAAAAAAAAAAAAAAAACACTTCAACTCCCTCCCCCATTTAGATTATTTATTAACATATTTTAAAAATCAGATGAGTTCTATAAATAATTTAGAGAAGTGAGAGTATTTATTTTTGGCATGTTTGGCCCACCACACAGACTCTGTGTGTGTATGTGTGTGTTTATATGTGTATGTGTGTGACAGGAAAATCTGTAGAGAAGAGGCACATCTATGGCTACTGTTCAAATACATAAAGATAAATTTATTTTCACACAGTCCACAAGGGGTATATCTTGTAGTTTTCAGAAAAGCCTTTGGAAATCTGGATCAGGAAATAGATACCATGGTTTGTGCAATTATGTAGTAAAAAAGGCAAATCTTTTCACCTCTGGCTATTCCTGAGACCCCAGGAAGTCAGGAAAAGCCTTTCAGCTCACCCATGGCTGCTGTGACTCCTACCAGGGCTTTCTTGGCTTTGGCGAAGGTCAGTGTACAGACATTCCATGGTACCAGAGTGCTCAGAAAGTCAAGATAGGATATGCCTCACCCTCAGCTACTCCTTGTTTTCTCTGAAGCACCCTGAAGGGTGCCATCCTTACAGAGCTAAGTGGAGACGTTTCCAGATCAGCCCAAGTTTACTATAGAGACTGGCCCAGGCACTGAATGTCTAGGACATGCTGTGGATGAAGATAAAGATGGTGGAATAGGTTTTATCACATCTCTTATTTCTCTTTTCCCCTTACTCTCTACCATTTCCTTTATGTGGGGAAACATTTTAAGGTAATAAATAGGTTACTTACCATCATATGTTCATATAGATGAAACTAATTTTTGGCTTAAGTCAGAACAACTGGCCCCCAATTGAAGTCATATTTGTGGGGGGAAATGGCATACGCAATATTATATTATATTGGATATTTATGTTCACACAGGAATTTGGTTTACTGCTTTGTAAATAAAAGGGAAAACTCCGGGTANOV28h, CG55256-05SEQ ID NO:652857 aaMW at 95006.1 kDProtein Sequence+TL,1MGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQESPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCGACLRPLAGLSLLPSPSWSPCPSPGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28i, CG55256-06SEQ ID NO:6534180 bpDNA SequenceORF Start: ATG at 163ORF Stop: TGA at 2704ATGGCCTCCCCGAACGTATGGGCCTCCCCGAGCGTATGGGCCTCCCCGAGCGTATGGGCCTCCCCGAGCGTATGGGCCTCACCCGAGCGTATGGGCCTCCCCGAGCGTATGGGCCTCCCCGAGCGTATGGGCCTCCCCGAGCGTATGGGCCTCCCCGAGCGTATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCAGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCAGGGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGCTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAACAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAAGATGTTTGGTTGCTGGGGGGGCTTTTTTGGGTTTTGGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTAAAAAAAAAAAAAAAACACTTCAACTCCCTCCCCCATTTAGATTATTTATTAACATATTTTAAAAATCAGATGAGTTCTATAAATAATTTAGAGAAGTGAGAGTATTTATTTTTGGCATGTTTGGCCCACCACACAGACTCTGTGTGTGTATGTGTGTGTTTATATGTGTATGTGTGTGACAGGAAAATCTGTAGAGAAGAGGCACATCTATGGCTACTGTTCAAATACATAAAGATAAATTTATTTTCACACAGTCCACAAGGGGTATATCTTGTAGTTTTCAGAAAAGCCTTTGGAAATCTGGATCAGGAAATAGATACCATGGTTTGTGCAATTATGTAGTAAAAAAGGCAAATCTTTTCACCTCTGGCTATTCCTGAGACCCCAGGAAGTCAGGAAAAGCCTTTCAGCTCACCCATGGCTGCTGTGACTCCTACCAGGGCTTTCTTGGCTTTGGCGAAGGTCAGTGTACAGACATTCCATGGTACCAGAGTGCTCAGAAAGTCAAGATAGGAAGGACACTTGGGTTGAATTCAGTAAGTTTCCTCTGAAGCACCCTGAAGGGTGCCATCCTTACAGAGCTAAGTGGAGACGTTTCCAGATCAGCCCAAGTTTACTATAGAGACTGGCCCAGGCACTGAATGTCTAGGACATGCTGTGGATGAAGATAAAGATGGTGGAATAGGTTTTATCACATCTCTTATTTCTCTTTTCCCCTTACTCTCTACCATTTCCTTTATGTGGGGAAACATTTTAAGGTAATAAATAGGTTACTTACCATCATATCTTCATATAGATGAAACTAATTTTTGGCTTAAGTCAGAACAACTGGCCCCCAATTGAAGTCATATTTGTGGGGGGAAATGGCATACGCAATATTATATTATATTGGATATTTATGTTCACACAGGAATTTGGTTTACNOV28i, CG55256-06SEQ ID NO:654847 aaMW at 93663.5 kDProtein Sequence+TL,1MGLPEPGPLRLLALLLLLLLLLLLQLQHLAAAAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATSLGTCRGDEFQCGDGTCVIAIKHCNQEQDCPDGSDEAGCLQVPPTFLGNRRRPRGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVKGYFKCECYPGYEMDLLTKNGACKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDTVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPPAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPAPLSELPWKSKRVALSLEDDGLPNOV28, CG55256-08SEQ ID NO:6552656 bpDNA SequenceORF Start: ATG at 103ORF Stop: TGA at 2428GCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGCGGCGGCGGCGGCGCGGGGAGCGCGGGGCGCGGCGGCCGGAGCCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGAGTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGCTGGCAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGGCCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTTAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGCCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGACGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCACGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGAGGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACNOV28, CG55256-08SEQ ID NO:656775 aaMW at 85584.3 kDProtein Sequence+TL,1MGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATSLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVGPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVLDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYPAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSNNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPARVALSLEDDGLPNOV28k, CG55256-09SEQ ID NO:6572623 bpDNA SequenceORF Start: ATG at 103ORF Stop: TGA at 2605GCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGCGGCGGCGGCGGCGCGGGGAGCGCGGGGCGCGGCGGCCGGAGCCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGATTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCMATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGCTGGCAAGAGCCCATCCCTAATCTTCACCAACCGGCACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAACCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGCCAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGACGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCNOV28k, CG55256-09SEQ ID NO:658834 aaMW at 92099.7 kDProtein SequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATSLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFATDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFANPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV281, CG55256-11SEQ ID NO:6594607 bpDNA SequenceORF Start: ATG at 237ORF Stop: TGA at 3126GCTGGCGGCGGCCGCCCAGGGCCGGGGCCGCGCGCCCAGCCTGAGCCCGCCCCGCCGCCGAGCGTCACCGAACCTGCTTGAAATGCAGCCGAGGAGCCGGGGCGGGCGGCAGCGGCGGCGGCGGCGGCGGCGGGGGCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGCGGCGGCGGCGGCGCGGGGAGCGCGGGGCGCGGCGGCCGGAGCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATCTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGAGTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACAGCGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACCAAGCAGGTGTGTCCTGCAGAGAAGCTGAGCTGTGGACCCACCAGCCACAAGTGTGTACCTGCCTCGTGGCGCTGCGACGGGGAGAAGGACTGCGAGGGTGGAGCGGATGAGGCCGGCTGTGCTACCTTGTGCGCCCCGCACGAGTTCCAGTGCGGCAACCGCTCGTGCCTGGCCGCCGTGTTCGTGTGCGACGGCGATGACGACTGTGGTGACGGCAGCGATGAGCGCGGCTGTGCAGACCCGGCCTGCGGGCCCCGCGAGTTCCGCTGCGGCGGCGATGGCGGCGGCGCCTGCATCCCGGAGCGCTGGGTCTGCGACCGCCAGTTTGACTGCGAGGACCGCTCGGACGAGGCAGCCGAGCTCTGCGGCCGCCCGGGCCCCGGGGCCACGTCCGCGCCCGCCGCCTGCGCCACCGTCTCCCAGTTCGCCTGCCGCAGCGGCGAGTGCGTGCACCTGGGCTGGCGCTGCGACGGCGACCGCGACTGCAAAGACAAATCGGACGAGGCCGACTGCCCACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCAAGGCTGCTGGTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGTACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCcGAAAGAGcAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGATGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAAGATGTTTGGTTGCTGGGGGGGCTTTTTTGGGTTTTGGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTAAAAAAAAAAAAAAAACACTTCAACTCCCTCCCCCATTTAGATTATTTATTAACATATTTTAAAAATCAGATGAGTTCTATAAATAATTTAGAGAAGTGAGAGTATTTATTTTTGGCATGTTTGGCCCACCACACAGACTCTGTGTGTGTATGTGTGTGTTTATATGTGTATGTGTGTGACAGGAAAATCTGTAGAGAAGAGGCACATCTATGGCTACTGTTCAAATACATAAAGATAAATTTATTTTCACACAGTCCACAAGGGGTATATCTTGTAGTTTTCAGAAAAGCCTTTGGAAATCTGGATCAGGAAATAGATACCATGGTTTGTGCAATTATGTAGTAAAAAAGGCAAATCTTTTCACCTCTGGCTATTCCTGAGACCCCAGGAAGTCAGGAAAAGCCTTTCAGCTCACCCATGGCTGCTGTGACTCCTACCAGGGCTTTCTTGGCTTTGGCCAAGGTCAGTGTACAGACATTCCATGGTACCAGAGTGCTCAGAAAGTCAAGATAGGATATGCCTGACCCTCAGCTACTCCTTGTTTTAAAGTTCAGCTCTTTGAGTAACTTCTTCAATTTCTTTCAGGACACTTGGGTTGAATTCAGTAAGTTTCCTCTGAAGCACCCTGAAGGGTGCCATCCTTACAGAGCTAAGTGGAGACGTTTCCAGATCAGCCCAAGTTTACTATAGAGACTGGCCCAGGCACTGAATGTCTAGGACATGCTGTGGATGAAGATAAAGATGGTGGAATAGGTTTTATCACATCTCTTATTTCTCTTTTCCCCTTACTCTCTACCATTTCCTTTATGTGGGGAAACATTTTAAGGTAATAAATAGGTTACTTACCATCATATGTTCATATAGATGAAACTAATTTTTGGCTTAAGTCAGAACAACTGGCCCCCAATTGAAGTCATATTTGTGGGGGGAAATGGCATACGCAATATTATATTATATTGGATATTTATGTTCACACAGGAATTTGGTTTACTGCTTTGTAAATAAAAGGGAAAACTCCGGGTATATGTNOV281, CG55256-11SEQ ID NO:660963 aaMW at 105714.3 kDIProtein SequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATLCAPHEFQCGNRSCLAAVFVCDGDDDCGDGSDERGCADPACGPREFRCGGDGGGACIPERWVCDRQFDCEDRSDEAAELCGRPGPGATSAPAACATVSQFACRSGECVHLGWRCDGDRDCKDKSDEADCPLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAGGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYPAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPAAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRWKRKNTKSMNFDNPVYR1CTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28m, SNP13382497 ofSEQ ID NO:6614294 bpCG55256-07,ORF Start: ATG at 103ORF Stop: TGA at 2818DNA SequenceSNP Pos: 2550SNP Change: T to CGCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGCGGCGGCGGCGGCGCGGGGAGCGCGGGGCGCGGCGGCCGGAGCCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATCTTGCGGCGGCAGCGGCTACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCAATCAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGAGTCACCCTGTGAGGGTCCCCGCAGATTTCAGTGTAAGAGTGGCAAGCGCGTGGACGGCGGGAAAGTGTGTGATGTGCAGAGGGACTGCCGGGACTGGTCGGATGAGCTTCTGAAAGTGTGGTGCGGTGCCTGTCTACGCCCACTGGCTGGACTCAGTCTCCTACCATCCCCCTCCTGGTATCTAGGCTCAAGGCCCTCCAGTGCCCCCTGCCCTGACACTTTCTGCTCTGACCCTCTCTTTGGATTCATGTGCCGTCCTATGGCTTCACATGGGGCTTTTCGCCCCCAGGCCTCTGGTCTACATCTCTACAAAGTGCTAAGAGCTTGTCCATCCCAGAGGAGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCTACGAGATGGACCTACTGACCAAGAACTGCCAAGCTGCTGCTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGTACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATCTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGACCTCCAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATCCACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAAGACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGACGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATCAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCGAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGATGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAAGATGTTTGGTTGCTGGGGGGGCTTTTTTGGGTTTTGGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTAAAAAAAAAAAAAAAACACTTCAACTCCCTCCCCCATTTAGATTATTTATTAACATATTTTAAAAATCAGATGAGTTCTATAAATAATTTAGAGAAGTGAGAGTATTTATTTTTGGCATGTTTGGCCCACCACACAGACTCTGTGTGTGTATGTGTGTGTTTATATGTGTATGTGTGTGACAGGAAAATCTGTAGAGAAGAGGCACATCTATGGCTACTGTTCAAATACATAAAGATAAATTTATTTTCACACAGTCCACAAGGGGTATATCTTGTAGTTTTCAGAAAAGCCTTTGGAAATCTGGATCAGGAAATAGATACCATGGTTTGTGCAATTATGTAGTAAAAAAGGCAAATCTTTTCACCTCTGGCTATTCCTGAGACCCCAGGAAGTCAGGAAAAGCCTTTCAGCTCACCCATGGCTGCTGTGACTCCTACCAGGGCTTTCTTGGCTTTGGCGAAGGTCAGTGTACAGACATTCCATGGTACCAGAGTGCTCAGAAAGTCAAGATAGGATATGCCTCACCCTCAGCTACTCCTTGTTTTAAAGTTCAGCTCTTTGAGTAACTTCTTCAATTTCTTTCAGGACACTTGGGTTGAATTCAGTAAGTTTCCTCTGAAGCACCCTGAAGGGTGCCAATCCTTACAGAGCTAAGTAAGACGTTTCCAGATCAGCCCAAGTTTACTATAGAGACTGGCCCAGGCACTGAATGTCTAGGACATGCTGTGGATGAAGATAAAGATGGTGGAATAGGTTTTATCACATCTCTTATTTCTCTTTTCCCCTTACTCTCTACCATTTCCTTTATGTGGGGAAACATTTTAAGGTAATAAATAGGTTACTTACCATCATATGTTCATATAGATGAAACTAATTTTTGGCTTAAGTCAGAACAACTGGCCCCCAATTGAAGTCATATTTGTGGGGGGAAATGGCATACGCAATATTATATTATATTGGATATTTATGTTCACACAGGAATTTGGTTTACTGCTTTGTAAATAAAAGGGAAAACTCCGGGTANOV28m, SNP13382497 ofSEQ ID NO:662905 aaMW at 100461.4 kDCG55256-07, ProteinSNP Pos: 816SNP Change: Asp to AspSequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTLGTCRGDEFQCGDGTCVNAIKHCNQEQDCPDGSDEAGCLQESPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCGACLRPLAGLSLLPSPSWYLGSRPSSAPCPDTFCSDPLFGFMCRPMASHGAFRPQASGLHLYKVLRACPSQRRLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCQAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPAAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28n, SNP13382498 ofSEQ ID NO:6634294 bpCG55256-07,ORF Start: ATG at 103ORF Stop: TGA at 2818DNA SequenceSNP Pos: 2783SNP Change: G to AGCAGCGGCAACCCCGGCGCCGCGGCAAGGACTCGGAGGGCTGAGACGAGGCGGCGGCGGCGCGGGGAGCGCGGGGCGCGGCGGCCGGAGCCCCGGGCCCGCCATGGGCCTCCCCGAGCCGGGCCCTCTCCGGCTTCTGGCGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCGGCTCCAGCATGTTGCGGCGGCAGCGGCTGATCCGCTGCTCGGCGGCCAAGGGCCGGCCAAGGAGTGCGAAAAGGACCAATTCCAGTGCCGGAACGAGCGCTGCATCCCCTCTGTGTGGAGATGCGACGAGGACGATGACTGCTTAGACCACACAGACGAGGACGACTGCCCCAAGAAGACCTGTGCAGACAGTGACTTCACCTGTGACAACGGCCACTGCATCCACGAACGGTGGAAGTGTGACGGCGAGGAGGAGTGTCCTGATGGCTCCGATGAGTCCGAGGCCACTTGCACACTGGGCACCTGCCGTGGGGACGAGTTCCAGTGTGGGGATGGGACATGTGTCCTTGCATCAAAGCACTGCAACCAGGAGCAGGACTGTCCAGATGGGAGTGATGAAGCTGGCTGCCTACAGGAGTCACCCTGTGAGGGTCCCCGCAGATTTCAGTGTAAGAGTGGCAAGCGCGTGGACGGCGGGAAAGTGTGTGATGTGCAGAGGGACTGCCGGGACTGGTCGGATGAGCTTCTGAAAGTGTGGTGCGGTGCCTGTCTACGCCCACTGGCTGGACTCAGTCTCCTACCATCCCCCTCCTGGTATCTAGGCTCAAGGCCCTCCAGTGCCCCCTGCCCTGACACTTTCTGCTCTGACCCTCTCTTTGGATTCATGTGCCGTCCTATGGCTTCACATGGGGCTTTTCGCCCCCAGGCCTCTGGTCTACATCTCTACAAAGTGCTAAGAGCTTGTCCATCCCAGAGGAGGCTGAACGAGTGTCTGCACAACAATGGCGGCTGCTCACACATCTGCACTGACCTCAAGATTGGCTTTGAATGCACGTGCCCAGCAGGCTTCCAGCTCCTGGACCAGAAGACCTGTGGCGACATTGATGAGTGCAAGGACCCAGATGCCTGCAGCCAGATCTGTGTCAATTACAAGGGCTATTTTAAGTGTGAGTGCTACCCTGGCAACGAGATGGACCTACTGACCAAGAACTGCCAAGCTGCTGCTGGAAAGAGCCCATCCCTAATCTTCACCAACCGGTACGAGGTGCGGAGGATCGACCTGGTGAAGCGGAACTATTCACGCCTCATCCCCATGCTCAAGAATGTCGTGGCACTAGATGTGGAAGTTGCCACCAATCGCATCTACTGGTGTGACCTCTCCTACCGTAAGATCTATAGCGCCTACATGGACAAGGCCAGTGACCCGAAAGAGCAGGAGGTCCTCATTGACGAGCAGTTGCACTCTCCAGAGGGCCTGGCAGTGGACTGGGTCCACAAGCACATGTACTGGACTGACTCGGGCAATAAGACCATCTCAGTGGCCACAGTTGATGGTGGCCGCCGACGCACTCTCTTCAGCCGTAACCTCAGTGAACCCCGGGCCATCGCTGTTGACCCCCTGCGAGGGTTCATGTATTGGTCTGACTGGGGGGACCAGGCCAAGATTGAGAAATCTGGGCTCAACGGTGTGGACCGGCAAACACTGGTGTCAGACAATATTGAATGGCCCAACGGAATCACCCTGGATCTGCTGAGCCAGCGCTTGTACTGGGTAGACTCCAAGCTACACCAACTGTCCAGCATTGACTTCAGTGGAGGCAACAGAAAGACGCTGATCTCCTCCACTGACTTCCTGAGCCACCCTTTTGGGATAGCTGTGTTTGAGGACAAGGTGTTCTGGACAGACCTGGAGAACGAGGCCATTTTCAGTGCAAATCGGCTCAATGGCCTGGAAATCTCCATCCTGGCTGAGAACCTCAACAACCCACATGACATTGTCATCTTCCATGAGCTGAAGCAGCCAAGAGCTGGAGATGCCTGTGAGCTGAGTGTCCAGCCTAATGGAGGCTGTGAATACCTGTGCCTTCCTGCTCCTCAGATCTCCAGCCACTCTCCCAAGTACACATGTGCCTGTCCTGACACAATGTGGCTGGGTCCAGACATGAAGAGGTGCTACCGAGCACCTCAATCTACCTCAACTACGACGTTAGCTTCTACCATGACGAGGACAGTACCTGCCACCACAAGAGCCCCCGGGACCACCGTCCACAGATGGACCTACCAGAACCACAGCACAGAGACACCAAGCCTGACAGCTGCAGTCCCAAGCTCAGTTAGTGTCCCCAGGGCTCCCAGCATCAGCCCGTCTACCCTAAGCCCTGCAACCAGCAACCACTCCCAGCACTATGCAAATGAGAACAGTAAGATGGGCTCAACAGTCACTGCCGCTGTTATCGGGATCATCGTGCCCATAGTGGTGATAGCCCTCCTGTGCATGAGTGGATACCTGATCTGGAGAAACTGGAAGCGGAAGAACACCAAAAGCATGAATTTTGACAACCCAGTCTACAGGAAAACAACAGAAGAAGAAGATGAAGATGAGCTCCATATAGGGAGAACTGCTCAGATTGGCCATGTCTATCCTGCAGCAATGAGCAGCTTTGATCGCCCACTGTGGGCAGAGCCCTGTCTTGGGGAGACCAGAGAACCGGAAGACCCAGCCCCTGCCCTCAAGGAGCTTTTTGTCTTGCCGGGGGAACCAAGGTCACAGCTGCACCAACTCCCGAAGAACCCTCTTTCCGAGCTGCCTGTCGTCAAATCCAAGCAAGTGGCATTAAGCCTTGAAGATGATGGACTACCCTGAGGATGGGATCACCCCCTTCGTGCCTCATGGAATTCAGTCCCATGCACTACACTCTGGATGGTGTATGACTGGATGAATGGGTTTCTATATATGGGTCTGTGTGAGTGTATGTGTGTGTGTGATTTTTTTTTTAAATTTATGTTGCGGAAAGGTAACCACAAAGTTATGATGAACTGCAAACATCCAAAGGATGTGAGAGTTTTTCTATGTATAATGTTTTATACACTTTTTAACTGGTTGCACTACCCATGAGGAATTCGTGGAATGGCTACTGCTGACTAACATGATGCACATAACCAAATGGGGGCCAATGGCACAGTACCTTACTCATCATTTAAAAACTATATTTACAGAGATGTTTGGTTGCTGGGGGGGGCTTTTTTGGGTTTTGGGGCATTTGTTTTTTGTAAATAAGATGATTATGCTTTGTGGCTATCCATCAACATAAGTAAAAAAAAAAAAAAAACACTTCAACTCCCTCCCCCATTTAGATTATTTATTAACATATTTTAAAAATCAGATGAGTTCTATAAATAATTTAGAGAAGTGAGAGTATTTATTTTTGGCATGTTTGGCCCACCACACAGACTCTGTGTGTGTATGTGTGTGTTTATATGTGTATGTGTGTGACAGGAAAATCTGTAGAGAAGAGGCACATCTATGGCTACTGTTCAAATACATAAAGATAAATTTATTTTCACACAGTCCACAAGGGGTATATCTTGTAGTTTTCAGAAAAGCCTTTGGAAATCTGGATCAGGAAATAGATACCATGGTTTGTGCAATTATGTAGTAAAAAAGGCAAATCTTTTCACCTCTGGCTATTCCTGAGACCCCAGGAAGTCAGGAAAAGCCTTTCAGCTCACCCATGGCTGCTGTGACTCCTACCAGGGCTTTCTTGGCTTTGGCGAAGGTCAGTGTACAGACATTCCATGGTACCAGAGTGCTCAGAAAGTCAAGATAGGATATGCCTCACCCTCAGCTACTCCTTGTTTTAAAGTTCAGCTCTTTGAGTAACTTCTTCAATTTCTTTCAGGACACTTGGGTTGAATTCAGTAAGTTTCCTCTGAAGCACCCTGAAGGGTGCCATCCTTACAGAGCTAAGTGGAGACGTTTCCAGATCAGCCCAAGTTTACTATAGAGACTGGCCCAGGCACTGATGTCTAGGACATGCTGTGGATGAAGGATAAAGATGGTGGAATAGGTTTTATCACATCTCTTATTTCTCTTTTCCCCTTACTCTCTACCATTTCCTTTATGTGGGGAAACATTTTAAGGTAATAAATAGGTTACTTACCATCATATGTTCATATAGATGAAACTAATTTTTGGCTTAAGTCAGAACAACTGGCCCCCAATTGAAGTCATATTTGTGGGGGGAAATGGCATACGCAATATTATATTATATTGGATATTTATGTTCACACAGGAATTTGGTTTACTGCTTTGTAAATAAAAGGGAAAACTCCGGGTANOV28n, SNP13382498 ofSEQ ID NO:664905 aaMW at 100433.3 kDCG55256-07,SNP Pos: 894SNP Change: Arg to GlnProtein SequenceMGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQESPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCGACLRPLAGLSLLPSPSWYLGSRPSSAPCPDTFCSDPLFGFMCRPMASHGAFRPQASGLHLYKVLRACPSQRRLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCQAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPAAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKQVALSLEDDGLP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 28B.

TABLE 28BComparison of the NOV28 protein sequences.NOV28a----------MGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKD(SEQ ID NO:638)NOV28b------------------------------------------AADPLLGGQGPAKDCEKD(SEQ ID NO:640)NOV28c----------------------------------------GSAADPLLGGQGPAKDCEKD(SEQ ID NO:642)NOV28dMPLSWPVSFPQRSLLPDPLELLCVLFVSFFLLLLQLQHLAAAAADPLLGGQGPAKDCEKD(SEQ ID NO:644)NOV28e------MGLPE----PGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKD(SEQ ID NO:646)NOV28f------MGLPE----PGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKDCEKD(SEQ ID NO:648)NOV28gMPLSWPVSFPQRSLLPDPLELLCVLFVSFFLLLLQLQHLAAAAADPLLGGQGPAKDCEKD(SEQ ID NO:650)NOV28h----------MGLPEPGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKD(SEQ ID NO:652)NOV28i------MGLPE----PGPLRLLALLLLLLLLLLLQLQHLAAAAADPLLGGQGPAKDCEKD(SEQ ID NO:654)NOV28------MGLPE----PGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKD(SEQ ID NO:656)NOV28k------MGLPE----PGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKDCEKD(SEQ ID NO:658)NOV281------MGLPE----PGPLRLLALLLLLLLLLLLRLQHLAAAAADPLLGGQGPAKECEKD(SEQ ID NO:660)NOV28aQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCADSDFTCDNGHCIHERWKCDGENOV28bQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCADSDFTCDNGHCIHERWKCDGENOV28cQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCADSDFTCDNGHCIHERWKCDGENOV28dQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPAAKKTPVRSDFTCDNGHCIHERWKCDGENOV28eQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCVRSDFTCDNGHCIHERWKCDGENOV28fQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCVRSDFTCDNGHCIHERWKCDGENOV28gQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPAAKKTPVRSDFTCDNGHCIHERWKCDGENOV28hQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCVRSDFTCDNGHCIHERWKCDGENOV28iQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCADSDFTCDNGHCIHERWKCDGENOV28QFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCADSDFTCDNGHCIHERWKCDGENOV28kQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCVRSDFTCDNGHCIHERWKCDGENOV28lQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCP--KKTCADSDFTCDNGHCIHERWKCDGENOV28aEECPDGSDESEATCTLGTCRGDEFQC--GDGTCVLAIKHCNQEQDCPDGSDEAGCLQE--NOV28bEECPDGSDESEATCTKQVC-----------------------------------------NOV28cEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28dEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28eEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATLCANOV28fEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28gEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28hEECPDGSDESEATCTLGTCRGDEFQC--GDGTCVLAIKHCNQEQDCPDGSDEAGCLQE--NOV28lEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28EECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28kEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATS--NOV28lEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATLCANOV28a------------------------------------------------------------NOV28b------------------------------------------------------------NOV28c------------------------------------------------------------NOV28d------------------------------------------------------------NOV28ePHEFQCGNRSCLAAVFVCDGDDDCGDGSDERGCADPACGPREFRCGGDGGGACIPERWVCNOV28f------------------------------------------------------------NOV28g------------------------------------------------------------NOV28h------------------------------------------------------------NOV28i------------------------------------------------------------NOV28------------------------------------------------------------NOV28k------------------------------------------------------------NOV28lPHEFQCGNRSCLAAVFVCDGDDDCGDGSDERGCADPACGPREFRCGGDGGGACIPERWVCNOV28a------------------------------------------------------------NOV28b------------------------------------------------------------NOV28c------------------------------------------------------------NOV28d------------------------------------------------------------NOV28eDRQFDCEDRSDEAAELCGRPGPGATSAPAACATVSQFACRSGECVHLGWRCDGDRDCKDKNOV28f------------------------------------------------------------NOV28g------------------------------------------------------------NOV28h------------------------------------------------------------NOV28i------------------------------------------------------------NOV28------------------------------------------------------------NOV28k------------------------------------------------------------NOV28lDRQFDCEDRSDEAAELCGRPGPGATSAPAACATVSQFACRSGECVHLGWRCDGDRDCKDKNOV28a-------SPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCGACLRPLAGLSLNOV28c-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28d-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28eSDEADCPLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQESPCEGPRRFQCKNOV28f-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28g-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28h-------SPCEGPRRFQCKSGKRVDGGKVCDVQRDCRDWSDELLKVWCGACLRPLAGLSLNOV28i-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28k-------LGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQG------------NOV28aLPSPSWYLGSRPSSAPCPDTFCSDPLFGFMCRPMASHGAFRPQASGLHLYKVLRACPSQRNOV28b------------------------------------------------------------NOV28c------------------------------------------------------------NOV28d------------------------------------------------------------NOV28eSGKRVDGGKVCDVQRDCRDWSDELLKVWCVSYHPPPG-----------------------NOV28f------------------------------------------------------------NOV28g------------------------------------------------------------NOV28hLPSPSWSPCP----SP-------------------G------------------------NOV28iG-----------------------------------------------------------NOV28------------------------------------------------------------NOV28k------------------------------------------------------------NOV28l------------------------------------------------------------NOV28aRLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28b------------------------------------------------------------NOV28c-LNECLHNNGGCSHICADLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28d-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCG--------------------NOV28e-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28f-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28g-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCG--------------------NOV28h-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28i-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQTCVNYKGNOV28-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28k-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28l-LNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGNOV28aYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28b------------------------------------------------------------NOV28cYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28d----------------------AGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALNOV28eYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28fYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28g----------------------AGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28hYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28iYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28YFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28kYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28lYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRYEVRRIDLVKRNYSRLIPMLKNVVALNOV28aDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28b-----------------------------------------------------P--AEK-NOV28cDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28dDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28eDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28fDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28gDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28hDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28iDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28DVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28kDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28lDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDNOV28aSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRNOV28b------------------------------------------------------------NOV28cSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRNOV28dSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRNOV28eSGNKTISVATVDGGRPRTLFSRNLSEPBAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRNOV28fSGN-----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------------------------------------------------------------NOV28cQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28dQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28eQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28fQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28gQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28hQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28iQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28QTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28kQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28lQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIANOV28aVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28b------------------------------------------------------------NOV28cVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28dVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPEAPDACELSVQNOV28eVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28fVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28gVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPPAPDACELSVQNOV28hVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28iVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28VFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28kVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28lVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQNOV28aPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAP-----------------NOV28b-------------------------------LGPDMKRCYFAP-----------------NOV28cPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAP-----------------NOV28dPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYEAP-----------------NOV28ePNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYEAPQSTSTTTLASTMTRTVPNOV28fPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRD------------------NOV28gPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAP-----------------NOV28hPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAP-----------------NOV28iPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAP-----------------NOV28PNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPNOV28kPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAP-----------------NOV28lPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPNOV28a------------------------------------------------------------NOV28b------------------------------------------------------------NOV28c------------------------------------------------------------NOV28d------------------------------------------------------------NOV28eATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANENOV28f---------------------------------------------------------ANENOV28g------------------------------------------------------------NOV28h------------------------------------------------------------NOV28i------------------------------------------------------------NOV28ATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANENOV28k------------------------------------------------------------NOV28lATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANENOV28a-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISNOV28b-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPPAPSISNOV28c-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRVPSISNOV28d-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISNOV28eDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKN-TKSMMFDNPVYRKTTEEEDNOV28fDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKN-TKSMMFDNPVYRKTTEEEDNOV28g-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISNOV28h-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISNOV28i-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISNOV28DSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKN-TKSMMFDNPVYRKTTEEEDNOV28k-QSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISNOV28lDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKN-TKSMMFDNPVYRKTTEEEDNOV28aPSTLSPATSNHSQHYANEDSKNGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWK-----NOV28bPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVTALLCMSGYLIWRNWK-----NOV28cP-----------------------------------------------------------NOV28dPSTLSPATSNHSQHAN-EDSKMGSTVTAAVIGIIVPIANEDSKMGSTVTAAVIGIIVPIGNOV28eE-----------------------------------------------------------NOV28fE-----------------------------------------------------------NOV28gPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIV-------------VIALLCMSGNOV28hPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWK-----NOV28iP-----------------------------------------------------------NOV28E-----------------------------------------------------------NOV28kP-----------------------------------------------------------NOV28lE-----------------------------------------------------------NOV28a----------------RKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSNOV28b----------------RKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSNOV28c---------------------------------------STLRPATSNHSQHYAN-----NOV28dDSPPVHEWIPDLEKLEAEEHQKHEFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSNOV28e---------------------------------------DELHIGRTAQIGHVYPAAISSNOV28f---------------------------------------DELHIGRTAQIGHVYP-----NOV28g-------YLIWRNWKRKNTKSMNFDNPVYRICTTEEEDEDELHIGRTAQIGHVYPAAISSNOV28h---------------RKNTKSMNFDNPVYRICTTEEEDEDELHIGRTAQIGHVYPAAISSNOV28i---------------------------------------STLSPATSNHSQHYAN-----NOV28---------------------------------------DELHIGRTAQIGHVYP-----NOV28k---------------------------------------STLSPATSNHSQHYAN-----NOV28l---------------------------------------DELHIGRTAQIGHVYPAAISSNOV28aFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSK-RVALNOV28bFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSK-RVALNOV28c------------------------------------------------------------NOV28dFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKQRVALNOV28eFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSK-RVALNOV28f------------------------------------------------------A-RVALNOV28gFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSK-RVALNOV28hFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSK-RVALNOV28i------------------------------------------------------------NOV28------------------------------------------------------A-RVALNOV28k------------------------------------------------------------NOV28lFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSK-RVALNOV28aSLEDDGLP----------------------------------------------------NOV28bSLEDDGLP----------------------------------------------------NOV28c--EDSKLE----------------------------------------------------NOV28dSLEDDGLP----------------------------------------------------NOV28eSLEDDGLP----------------------------------------------------NOV28fSLEDDGLP----------------------------------------------------NOV28gSLEDDGLP----------------------------------------------------NOV28hSLEDDGLP----------------------------------------------------NOV28i--EDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEENOV28SLEDDGLP----------------------------------------------------NOV28k--EDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEENOV28lSLEDDGLP----------------------------------------------------NOV28a------------------------------------------------------------NOV28b------------------------------------------------------------NOV28d------------------------------------------------------------NOV28e------------------------------------------------------------NOV28f------------------------------------------------------------NOV28g------------------------------------------------------------NOV28h------------------------------------------------------------NOV28iEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSNOV28------------------------------------------------------------NOV28kEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSNOV28l------------------------------------------------------------NOV28a-------------------------------NOV28d-------------------------------NOV28e-------------------------------NOV28f-------------------------------NOV28g-------------------------------NOV28h-------------------------------NOV28iQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28-------------------------------NOV28kQLHQLPKNPLSELPVVKSKRVALSLEDDGLPNOV28l-------------------------------


Further analysis of the NOV28a protein yielded the following properties shown in Table 28C.

TABLE 28CProtein Sequence Properties NOV28aSignalP analysis:Cleavage site between residues 33 and 34PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 1; neg. chg 1H-region:length 14; peak value 11.70PSG score:7.30GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 4.70possible cleavage site: between 26 and 27>>> Seems to have a cleavable signal peptide (1 to 26)ALOM:Klein et al's method for TM region allocationInit position for calculation: 27Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 768-784−13.80PERIPHERALLikelihood = 2.81 (at 203)ALOM score: −13.80 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 13Charge difference: −0.5 C(0.5)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 785 to 905)MITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):5.68Hyd Moment(95):4.45G content:2D/E content:2S/T content:0Score: −6.43Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 35 LRL|QHNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PVVKSKR (3) at 888bipartite: nonecontent of basic residues: 10.3%NLS Score: −0.22KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: extracellular, including cell wall22.2%: Golgi22.2%: endoplasmic reticulum11.1%: plasma membrane>> prediction for CG55256-07 is exc (k = 9)


A search of the NOV28a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 28D.

TABLE 28DGeneseq Results for NOV28aNOV28aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAU91290Human NOV5i protein - Homo sapiens,1 . . . 905903/905 (99%)0.0905 aa. [WO200216600-A2, 28-FEB-2002]1 . . . 905903/905 (99%)AAU91288Human NOV5g protein - Homo sapiens, 857 aa.1 . . . 905851/905 (94%)0.0[WO200216600-A2, 28-FEB-2002]1 . . . 857854/905 (94%)ABP56838Human apolipoprotein E receptor 2 protein1 . . . 905800/973 (82%)0.0SEQ ID NO: 5 - Homo sapiens,1 . . . 963817/973 (83%)963 aa. [WO200299438-A2, 12-DEC-2002]AAU91289Human NOV5h protein - Homo sapiens,1 . . . 905786/908 (86%)0.0847 aa. [WO200216600-A2, 28-FEB-2002]1 . . . 847802/908 (87%)AAU91285Human NOV5d protein - Homo sapiens,42 . . . 905704/884 (79%)0.01012 aa. [WO200216600-A2, 28-FEB-2002]162 . . . 1012724/884 (81%)


In a BLAST search of public sequence databases, the NOV28a protein was found to have homology to the proteins shown in the BLASTP data in Table 28E.

TABLE 28EPublic BLASTP Results for NOV28aNOV28aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ14114Apolipoprotein E receptor 21 . . . 905800/973(82%)0.0precursor - Homo sapiens1 . . . 963817/973(83%)(Human), 963 aa.Q921B6ApoE receptor-2 - Mus musculus1 . . . 905719/912(78%)0.0(Mouse), 870 aa.1 . . . 870766/912(83%)O14968ApoER2delta4-7 - Homo sapiens1 . . . 905721/907(79%)0.0(Human), 793 aa.1 . . . 793736/907(80%)Q924X6Apolipoprotein E receptor 21 . . . 905689/1011(68%)0.0precursor - Mus musculus1 . . . 996743/1011(73%)(Mouse), 996 aa.JE0237apolipoprotein E receptor 21 . . . 905689/1009(68%)0.0precursor- mouse, 996 aa.1 . . . 996742/1009(73%)


PFam analysis predicts that the NOV28a protein contains the domains shown in the Table 28F.

TABLE 28FDomain Analysis of NOV28aIdentities/SimilaritiesNOV28a Matchfor theExpectPfam DomainRegionMatched RegionValueIdl_recept_a 45 . . . 8320/43 (47%)7.7e−1332/43 (74%)Idl_recept_a 84 . . . 12419/43 (44%)2.8e−1332/43 (74%)Idl_recept_a125 . . . 16319/43 (44%)1.2e−1132/43 (74%)Idl_recept_a165 . . . 20720/44 (45%)1.3e−0733/44 (75%)EGF282 . . . 31614/47 (30%)  9e−0627/47 (57%)TIL273 . . . 32213/67 (19%)0.3329/67 (43%)EGF322 . . . 35615/47 (32%)0.5 27/47 (57%)Idl_recept_b404 . . . 44917/48 (35%)1.2e−1040/48 (83%)Idl_recept_b451 . . . 49223/47 (49%)2.3e−1540/47 (85%)Idl_recept_b494 . . . 53619/47 (40%)  2e−1538/47 (81%)Idl_recept_b538 . . . 58121/47 (45%)2.9e−1239/47 (83%)Idl_recept_b582 . . . 62212/47 (26%)1.5e−0837/47 (79%)


Example 29

The NOV29 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 29A.

TABLE 29ANOV29 Sequence AnalysisNOV29a, CG55776-01SEQ ID NO:6657876 bpDNA SequenceORF Start: ATG at 6ORF Stop: TGA at 7857TCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGAACGCAATGAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTACAAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCAATTTTCACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAACACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGACAATGTGCTCTATCAGTCATCAAAGAGACAAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCAACAAGTAAGAGGCACACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAACTACAGCCATGTCAAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAGAGAGCATTTCCAAAGTAGACCCCCAATAACAAGTAAGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGAAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCATTGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAGTTAGTTTACAAAAAAGCACAACATGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACATCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCAACACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAIACAGTTGTCTCAGAATCATCCCAGGGAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGATTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAGGACTGAAGTGAAATTTTGGGGACAAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTTATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGAATGATCTGATACTGATGCATGTTAGCCTAAGACTGAPAACCTGCCAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAGATGAAATGAAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAACCAAACAAGAGAATCAAAAGCTGGAGACACACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAAATGGGTCTTTGACCAATCAACAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29a, CG55776-01SEQ ID NO:6662617 aaMW at 290200.2 kDProtein SequenceMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHNDHIANIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKSFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNNTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKKAAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRTLQVTPKDQGYYRCVAPAPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLPRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERATTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIAILASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMARLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLnTITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKNKLLDSGEVCVARNPSGDDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLAEMLRRPTFRNPFNEKMAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITRPPRSIVTRTGAAFQLHCVALAGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29b, 248210167SEQ ID NO:667475 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGACTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTGATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTCTCGAGGGCNOV29b, 248210167SEQ ID NO:668158 aaMW at 18415.2 kDProtein SequenceTLENVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPLEGNOV29c, 247679561SEQ ID NO:6692011 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceAGGCTCCGCGGCCGCCCCCTTCACCCTCGAGGGGGACAATTACTACTGAAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTACAGAAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGGATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGAGATCCCAAAACCAAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAATGTACAAACTGGATGTGGTCTCTAAACCTCCAATTAATCAAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAACTATATCTGTAAGGCTCAAAAAAAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAATCGTCCAACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGcCTTGGGAGTTCCCAAGCCAGAATCACATGGGAQATGCCTGACCACTCCCTTCTCTCAAACGGCAAAGTAAGAGAGGACACATGGAAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGATCCCCAAAQCTCCGATTCTGGGATATACAAATGCACAGCACTCGAGGGCAAGGGTGGGCGCGCCNOV29c, 247679561SEQ ID NO:670670 aaMW at 73997.1 kDProtein SequenceGSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRIIVHKNGTLERIAVRLSDSADFICVARNEGGESVLVVQLEVLEMIRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRIITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTALEGKGGRANOV29d, 248057904SEQ ID NO:671771 bPDNA SequenceORF Start: at 1ORF Stop: end of SequenceAAGCTTGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATAAACCCATGGAAACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGATGTATAAATGCAAAAAGAATAAGAAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCCTCGAGNOV29d, 248057904SEQ ID NO:672257 aaMW at 29661.2 kDProtein SequenceKLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYNPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCALENOV29e, 248057927SEQ ID NO:673771 bPDNA SequenceORF Start: at 1ORF Stop: end of SequenceAAGCTTGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAIAGCCAGATGTAATAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGTAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCCTCGAGNOV29e, 248057927SEQ ID NO:674257 aaMW at 29661.2 kDProtein SequenceKLACPPRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKAHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPSSAQQCPLCNNPRTSKGKPLAMVSAAAFQCALENOV29f, 249239821SEQ ID NO:675771 bPDNA SequenceORF Start: at 1ORF Stop: end of SequenceAAGCTTGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCATGTGGAACGCATCAATTTAAGGAATAACACAATTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAATGAAGCTATAAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTGATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCMAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGATGTAATAAATGCAAAAAGATAAGAGTCCCTCTAAAGTGCTCCGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGAGCTTTCCAGTGTGCCCTCGAGNOV29f, 249239821SEQ ID NO:676257 aaMW at 29582.1 kDProtein SequenceKLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHINNIFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPSSAPQCPLCMNPRTSKGKPLAMVSAAAFQCALENOV29g, 248057920SEQ ID NO:677771 bPDNA SequenceORF Start: at 1ORF Stop: end of SequenceAAGCTTGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTACACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTGATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAACTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGAACCTGTGATTGCCATTTAAGTGGTTGTTTGACTGGATACAGGAGAAGCCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCCTCGAGNOV29g, 248057920SEQ ID NO:678257aaMW at 29673.2 kDProtein SequenceKLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLAMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLFDWIQEKPDVIKCKKDRSPSSAQQCPLCAPRTSKGKPLAMVSAAAFQCALENOV29h, 247679541SEQ ID NO:6792059 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceAGGCTCCGCGGCCGCCCCCTTCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGAAACTGAAACCTGCCAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAAGTAGATTGCALAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAAGCGGAGGAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGAGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAGTGAAACTGCTCGATTCTGGAAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGAATGACACCAAAATGTAACAACTGGAATGTGGTCTCTAACCTCCATTATCAATGGTCTGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATATCTGGATTTTAACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCAAGGGTGGGCGCGCCNOV29h, 247679541SEQ ID NO:680686 aaMW at 75732.1 kDProtein SequenceGSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVAPNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFTISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAIAPLGSDYAATYIQVILEGKGGRANOV291, 249116954SEQ ID NO:6812011 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceAGGCTCCGCGGCCGCCCCCTTCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAAGTATATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAGTTATCAGTATCTGATAGCAAGCAAATGGTTCTTTTATCAATTTCTAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTACTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCACTCGAGGGCAAGGGTGGGCGCGCCNOV29i, 249116954SEQ ID NO:682670 aaMW at 73997.1 kDProtein SequenceGSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMTSFSIDRYTFHANGSLTINKVKLLDSGEYVCVAAAPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWTMPDNIFLTAPYYGSRITVHINGTLEIRNVRLSDSALFICVARIEGGESVLVVQLEVLEMLRRPTFPAPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAAAAKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTALEGKGGRANOV29, 248210155SEQ ID NO:6831420 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGAATTCTGGAGAGTACGTATGTGTAGCCCGAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAACAACTTTGAACTGCAGAGCTGAGGGACACCATCTCCTGAAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTAAGAAGAACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGATCTCTATCACTGCATTGTGTGTCTGATGGAAATCCCTAAGCCAAAATATCATGGACTAAATGCCAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCAATGACATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAATAAGTGTTGGTCATACACAGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAGTGAGCAAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29, 248210155SEQ ID NO:684473 aaMW at 52119.3 kDProtein SequenceTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTIYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIVVPDNIFLTAPYYGSRTVHKNGTLEIRNVRLSDSADFICVARNEGGESVLWQLEVLEMLRRPTFRIAAPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAG1ARCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGNOV29k, 248213764SEQ ID NO:6851372 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGAATTCTGGAAGAGTACGTATGTGTAGCCCGATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCAATTTAAATGAAATAAAGTTGCCCAGCTAGA3AGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTAATCATTTCTAAAACACTCGGGAGGATGCAGGAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAAAATTAGTCATATTAGAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACATCGTCCACCCAAGGAAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCACGGCAAGTAAAGAGAGGACACATCGAAGTGAAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCACTCGAGGGCNOV29k, 248213764SEQ ID NO:686457 aaMW at 50532.6 kDProtein SequenceTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHHSKKHFDCRAGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFPNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHcVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHRSEQLHLQGTLVIQNPQTSDSGIYKCTALEGNOV29l, 248213768SEQ ID NO:6871383 bPDNA SequenceORF Start: at 2ORF Stop: at 1382CACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAATGTACAAACTGGAATGTGGTCTCTAAACCTCCATTAATCAAATGGTCTGTAATACATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAACACTTTGACTGCAGAGCTGAAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCGGAAATMATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTAATCATTTCTAAACAAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCACTCGAGGGCAAGGGCGAATCNOV29l, 248213768SEQ ID NO:688460 aaMW at 50747.8 kDTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNLVRLSDSADFICVAPNEGGESVLWQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHVSDGIPKPNIKWTMPSGYAATDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVAMVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTALEGKGENOV29m, 248213772SEQ ID NO:6891372 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAAAATTACTACTGAAACTGCTCAGCCACTGGGGAGCCCACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATGGGTCGTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGcCCAGCTGGGAAAAGTCCACAGATTGAATTGCTCTGTTGATGGTAACCCACCACCGGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGATTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCAACCAGGGACAGTAAAGAATCAAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAATGGACTATGCCAAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAAGCCAGAATCACTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAACCTCCGATTCTGGGATATACATGCACAGCACTCGAGGGCNOV29m, 248213772SEQ ID NO:690457 aaMW at 50433.5 kDProtein SequenceTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRYTFGSLTINKKAKAALDSGEYVCVAPANPSGDDTKMAYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCAGTPSPEVMWIMPDNIFLTAAPYYGSRITVKKNGTLEIRNVRISDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIIWEMPDHSLLSTASKERTHGSEQLHLQGTLVTQNPQTSDSGTYKCTALEGNOV29n, 248586774SEQ ID NO:6911420 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCCCCCAAAAAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATGGGTCTTTGACCAATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAATCCCAGTGGGGATGACAACCAAATGTACAAACTGGAATGTGGTCTCTAAACCTCCAATTAAATCATGGTCTGTATACATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACAAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAAATGAAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAAACCCACCACCTGAAATAATCTGGATTTTACCAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAAATATCGCTGTGCAGCTAGGAATAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAPAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29n, 248586774SEQ ID NO:692473aaMW at 52168.4 kDProtein SequenceTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVVSKPPLINGLYTNRTVIKATAVPASKAFDCFAAGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRVRLSDSADFICVAR1AIEGGESVLVVQLEVLEMLRKPTFRNPFNEIVAQLGKSTALINCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGNOV29o, 248586793SEQ ID NO:6931420 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATGGGTCGTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAACTGGATGTGGTCTCTAACCTCCATTAATCAATGGTCTGTATACCAAAACAGAACTGTTATTAAAGCCACAGCTATGAGACATTCCAPAAACACTTTGACTGCAGAGCTGAAGGGAACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGATACTGGAAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCGGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAAAGAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAGGCATCAAGTGGAAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACATGGCACCTTAGTCAATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAGCCAGAAATCAACGTGGGAGATGCCTGACCACTGGGTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTAGATAATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29o, 248586793SEQ ID NO:694473 aaMW at 52200.5 kDProtein SequenceTLEGDKLLLNCSATGEPKPQIMWRTLPSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAMRHSKKIFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHNGTLEIRNKTRLSDSADFICVARNEGGESVLVQLEVLEMLRRPTFRIAPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGNOV29p, 248586820SEQ ID NO:6951420 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAGTCATGTGGATCATGCCAGACATATTTTCCTCACAGCCCCAATAACTATGGAAGCAGAATCACAGTCCATATATGGAACCTTGGAATTAGGAATGTGAGGCTTTCAGAATTCAAGCCGAACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGGCATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCGGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGATTACAATCGTCCACCCAGGAGTAAATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACANOV29p, 248586820SEQ ID NO:696473 aaMW at 52169.4 kDProtein SequenceTLEGDKLLLNCSATGEPKPQIMWRLQSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVAATSKKHFDCPAGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARIAIEGGESVLWQLEVLEMLRRPFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGNOV29q, 248586824SEQ ID NO:6971420 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGGGGGACAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAAAACACCAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGTACACATTTCATGCCAATAAGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAATCCCAAGTGGGGAGAACTGTTATTAAAGCCACAGCTGTGAAGACAAATTCCAACAACTTTGAAACTGCAGAAATGAAGACACCATCTCCTGAAGTCATGTGGATCAATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAGCAGAATCACAGTCCATAAAAAAATGGAACCTTGGATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTAAGAAGACCGACATTTAGAAAATCCAATTTAATGAAAAAATAGTTGCCCAGCTGGGAGTCCACAGCATTGATTGCTCTGTTGATGGTAACCCACCAAACCTGAAAAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAACAAACTCGGGAGGATGCAGGAATATCGCTGTGCAGCTAGGAATAAGTTGGCTAAATAAATTGAGATTAAGTAATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAAGTAAAGGCATCAGTGGAGGGTTATGTAGTAGACAGGCCTCAATTAATGGGAATACATATTGACATGACAAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATATGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAAATTACAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAAGCCAGAATCACGTGGGAGATGTCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29q, 248586824SEQ ID NO:698473 aaMW at 52198.4 kDProtein SequenceTLEGDKLLLNCSATGEPKHQIMWRLPSKAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALINCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLISNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGNOV29r, 247679817SEQ ID NO:699635 bPDNA SequenceORF Start: at 3ORF Stop: end of SequenceAGCTCCGCGGCCGCCCCCTTCACCCTCGAGTGTCCTCGCCGCTGTGCCCTGTTATATGCACCCTCGAGTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTAGATTGATGGCCTGACAAGACCTTCTCAGATTTGCAAAGGCCTTGCAAGGTCTTAAATGAAGCTATAACAAAGTCAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATACCCAAGAAAGGTTTTTGAAAAGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAGGATCAGCTCACTAAGCTCCACCCAGATACAAATTTGTCTCTTTGAGCTACCTCCAGATATTTAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTCTCGAGGGCAAGGGTGGGCGCGCCNOV29r, 247679817SEQ ID NO:700211 aaMW at 24064.7 kDProtein SequenceAPRPPPSPSSVLAAVPVICTLEPPACACYMPTEKAACTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPLEGKGGRANOV29s, 248210264SEQ ID NO:701646 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceAACCCTCGAGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCA1ATGTGGAACGCATCATTTAGGATACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGcAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTCTCGAGGGCNOV29s, 248210264SEQ ID NO:702215 aaMW at 24618.6 kDProtein SequenceATLENKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQTFKISFIKFLYLSDNFLTSLPQEMVSYMPLEGNOV29t, 248210551SEQ ID NO:703646 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAATGAGCTATAAACAAAAGTCCGAAACTTCAGAAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTCTCGAGGGCNOV29t, 248210551SEQ ID NO:704215 aaMW at 24618.6 kDProtein SequenceTLEMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNEFINPEVFYGLIAFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPLEGNOV29u, 248210824SEQ ID NO:705646 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAATCTCTTTCATTAGTTCCTATACTTGTCTGAT1AAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTCTCGAGGGCNOV29u, 248210824SEQ ID NO:706215 aaMW at 24618.6 kDProtein SequenceTLEMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPLEGNOV29v, 248679541SEQ ID NO:707646 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTCTCGAGGANOV29v, 248679541SEQ ID NO:708215 aaMW at 24618.6 kDProtein SequenceTLEMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPLEGNOV29w, 247679454SEQ ID NO:7092011 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceAGGCTCCGCGGCCGCCCCCTTCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAGAACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAAATATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGTATGTGAGGCTTTCAGATTGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGAACCACAAGTTATCAGTATCTGAATAGCAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCAACCAAGGGACAGTAAGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAAGCCAAATATCAAATGGAACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAATACATATTGCATGACAATGGACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATAATCTGTAAGGCTCAAAATAGTGTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCACTCGAGGGCAAGGGTGGGCGCGCCNOV29w, 247679454SEQ ID NO:710670 aaMW at 74046.2 kDProtein SequenceGSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDENKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRYVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEIKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTMAEGKGGRANOV29x, 314361407SEQ ID NO:7112902 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCCTCGAGAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGACTTGATTTATCTAAAGAGGAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTAACCAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTAATTCTAAAGAGCAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAGAGTAGTAAATATTTTGGGGACAATTACTACTGAAACTGCTCAAGCCACTGGGGAGCCCAAACCCCAAATATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTAACCCTATGGTGGGGGATGATCTGATACTGATGCATGTTAAGCCTAAGACTGACCTGCCAAATTGAACCAAACAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGAATTTCCAAGTAGATTGCAAGCTTCCGGCTCCCCAGTGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACAACCCTAGGGAAGAATGATGAAAGGTCCAACTTACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAAACCAACAAAGAGAATCAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAACCAAAATATTTTGGTTGCTGCCTTCCATAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCATGGGTCTTTGACCATCAAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAATAGAACTGTTATTAAACATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGAAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAAATTGCTCTGTTGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGATCTCTATCAACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAGTGGTTATGTAGTAAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29x, 314361407SEQ ID NO:712967 aaMW at 106911.3 kDProtein SequenceTLENSDAFLPCEAVGNPLPTIHWTRVPSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTAMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRAVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKAPLGSDYAATYIQVILEGNOV29y, 317803448SEQ ID NO:7132902 bPDNA SequenceORF Start: at 2ORF Stop: end of SequenceCACCAGATCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAGAGATCACAGTTCATTCCGGAGCACTGTGGAAACTGAAGTGCAGAAGCAGAGGTAAGGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAAATCTCAGTATTTATGACCGTGATTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAATACAAAGTCATTGCAGCATCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCAAGCGAAGAGTAGTAAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAAGGATAGAAGCTGCAATCCCAGAGGAAACTGAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGTATTTTAGAAAGCAAAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAGCTTCCGGCTCCCCAGTGAGGAAGGAAGAAATTATACTTGCTATGCCCAAAAGAACACCCTAGGGAAGATGTGAGGTCCACTTACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACAGACATGATTTCCTTCTCCATTGATAGGTACACAAAATTTCAATGCCATGGGTCTTTGACCATCACAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAAATCCCAGTGGGGATGACACCAAATGTGCCACAGCTGTGAGACATTCCMACACTTTGACTGCAGAGCTGAAAAGGGAACAAACCATCTCCTGAGTAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAATGGTAACCCACCACCTGAATAATCTGGATTTTACCATACACAACGATTTTCCAATGGAACCAAACAAAGTTATCAGTATCTGATAGCAAAGCAAATGGTTCTTTTAATCAAAATTTCTAACACTCGGGACATGCAAGAAGCCAGTTATTCTTACCTAATGCAACCAAGGGACAGTAAGGCATCAGTGGAGATCTCTATCACTGCAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTAATGACAGAGGAAACTATATCTGTAAGGCTCAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAAGCCAGAAACACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAGAGAGGACACATAAGTGAGCAGCTTCACTTACGAATAAATACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGAATATAAATGCAAACAGCAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTATCCTCGAGGGCNOV29y, 317803448SEQ ID NO:714967 aaMW at 106912.3 kDProtein SequenceTRSNSDAFLPCEAVGNPLPTIHWTRVPSGLDLSKRKQNSRVQVLPNGTLSIQRAEIQDRGQYLCSASNLFGTDHLHTLSVVSYPPRILERRATKEITVHSGSTVELKCPGRPSPTVTVVIAAAAQTVVSESSQGSRQAVATVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIPNAASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAAASQKATEVNFGDKLLLNCSATGEPKPQIMIPSAWDQQHRVGSWIHVYAAYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRTRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVVGHTLITVPVMIVAYPPRITAPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGNOV29z, CG55776-02SEQ ID NO:7157831 bPDNA SequenceORF Start: ATG at 6ORF Stop: TGA at 7812TCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAAGACCTTCTCAGATTTGCAGGCCTTGCAATGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAAACTTCAGAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAAAGCCTTTACCTGCATGGACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTTTTACCAGATGTAATAATGCAAAAAGATAGAAGTCCCTCTAGTGCTCAAAGCAAGTGTCCAACTTTAATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGGAGGCAAAGATCTCAAGAAGCAGATCCCTCTTGGTTATGCAGACCTTTCCTTGCAAGCTGAAACAGACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGGTCTTGGTAGGTGGACCGTTGGCCTGAACTGCCCAGGCCAGGAGACCCCACCCCAACACGTGGAATTGGCTTCTAGCTGATGGAAGTAGTGAGAGCCCCTTATGTCAGTGAGGATGGACAATCCTAAATAAGAACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCATTATGATGATGCAGATATTCTCACCTATAGGATACTGTGGTAAGAAACCTTTGGTCGAGCCTAATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTAAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCA7AGATGAGGACAAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCAGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAAGGAAATTCCCTGGCAAACAGAAACTTTGTATAACCATAACCCAAAGGCAGATTAAGGAATCAACATAAAGTTAAAGTTTACAAAAAGCACAGCTGTGATGCTTCCTAAACATCTCCTGCTTTACCACAAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCAACTCTTAATCTGACAGATGTGATTGAGAACTAGCCCAAGCAAGTACTCAAGACTTTGAAGAGCACAACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTACCACAGCATTAGTAAGCAGAAGGTTAAAAACATCCCCCAAGGTTCACCCAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAAATCTGTTACATTCTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGAATGGAACAGAGACACAAAGAAGAGTGACTTTGATAAGAACCAGTTCAAGAAGCAACAACTTCCAAAACTCCTTCCCTTTGACTCTTCCCATCAGGTATGTCAGGACTTGAATTTAAATCTAAGAGGCAGAAATAGCAAGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAAACAAAGTCATTGCAGCACTGTACTGCAAAGGAACTCCTCAAGCCCAAGCGTTTAAACTGGGTCCTCTCTGATGGCACTGAGTGACCATTACAGTTTACCATTCCAAGTTGTTCTTAATTTTCAAATGGGAACTTTGAATATAGAAAQCTAGCCTCTTCAGACAGGGGCACTTATGATGCATTGCTACCAGTTCCAACTGGTTCGGAAGCGAGAGTAAGTATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGTAAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTTATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGACCTGCCAAATTGAACCAACAAGCAAGTATTTTAGAAAGCAAGTGCTCCATGGGAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATCCCTTTTCAACAATGGACTTTATACTTCAAACAAAGTTGGGGTAAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACCGTGAGGTGACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGGGTCTTTGACCATCAAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAATCCCAGTGGGGATGACACCAAATGTAACAAACTGGAAATGTGGTCTCTACCTCCATTATCAAATGGTCTGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAAGAACAATTCCAAACACTTTGACTGCAGAGCTGGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAAGCGTGTTGGTAGTACAGTTAGAGTACTGGAAATGCTGAGAAGACCGACATTTAGAATCCATTTAATGAAATAAAGTTACCAGCTGGGATAAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTACCCACCACCTGTAATCTGGAAATTTTAAACCAAATGGCACACGATTTTCCAATGGACCACAGTTATCAGTATCTGATAGCAGCAATGGTTCTTTTAATCAATTTCTAAAAACAACTCGGGAGGATGCAGGAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTAATATTGAGAAAATTAGTCATATTAGAATTGGCCAGAAGCCAGTTATTCTTACCTATGCAACCAGGGACAGTAAAGGCAATTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAATCGTCCAACCCATGCCAAGTGGTTATGTAGTAGACAGGCCTCATTATGGGATACATAATTGCATGAAACAATAAACAACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAACTATATCTGTA3AGGCTCAAATAAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAAATTACATCGTCCAACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATGTGACATGAAATAATAAAGTCNOV29z, CG55776-02SEQ ID NO:7162602 aaMW at 288454.2 kDProtein SequenceMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEAHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHIPDKTFSDLQALQVRLMVLKMSYNKKTRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVAHLEGNQLTKLHPAFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEASYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYNTAPDVIKCYAADRSPSSAQQCPLCMNPRTSKGKPLAMKTSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFSPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPKAWQILAYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKAHSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQBWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIRHSTIPPFLSSSATLMPVPISPPFTQRAKTTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNNMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDREVTGDPKPKIFWLLPSNGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29aa, CG55776-03SEQ ID NO:7178270 bPDNA SequenceORF Start: ATG at 6ORF Stop: TGA at 7779TCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATAACTTTTTAATGGCCTCAAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCAATTTTCAACATTTTTGGTGTGCACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGAACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTCAGTCAAGATGAAAGGAACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAAGGTTGGAAACAAACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAAGTGACTGCTGACTCCAGAACAATAATCTGATAGTCCTATGACAACATAAATTATGGCACAGAAATTCTCTCCTGTTGTGATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAAGTCTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAGATGTCAATGTCAAAATGCTTAAGTAAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCACCATAACCCAAAAGGCAGATTAAAGGAAATCAACAATAAAGTTAGTTTACAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAGACTCAAGTAACAAGCAATCAATAATCAACGCAAACAAGCAAATACCAGCACACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCAACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAACAACAACTGGGAATTTCAAGCACAATCAGTTTTCATTCAGAACTCTTATCTGACAGATGTGATTGAAAGAAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACATTGCTTCTGAAACAACTTTGTCCAGCAATCACACCAGAGTACCACAACTAGGAAAGCAAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTACCAACAAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTACAACAGTTAAATCAACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAAGAAATTGCTGAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAGCCCAGGAAAATAGTTGGAAAAGAGCTGCAAGTTTTAACTATTCCAATAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTAATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAATAACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAGGAGGCAAGTCATTGTAAGGCAACTTGGGGTGAAAGTTTAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAAGCTGCATCCCAGAAGGACTGAAAGTGAATTTTGGGGACATTACTACTGAACTGCTCAGCCACTGGGGAAGCCCAAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAGACAAGTGGTGTCTAACTTGTGTGTGGCAAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAGAACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAAATGCAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAACCMACAAGAGAAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCATTCATGCCAAATGGGTCTTTGAACCATCAACAAGTGAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGAATGACACCAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTAATCTGTGTGGCCCGAAATGAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAAATGCTGAAGAAGACCGACATTTAGATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAATGGCACACGATTTTCCAATGGACCACAAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAACAACTCGGGAGGATGCAGGAAAAAATCATGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAMATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAGGCATCAGTGGAGAAACTCTAATCAACTAATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAGGTACCCTAAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCAACAACATCTGGGCAGAATTTATTTTTTGGAAGAAGTTTAATCAAGGCAGCCATAGGCATGTAAATGATTTGAATAACAATTTACAGTATTAAATTTACAATGAACATGCAAAATAAAAGGACTTGTAAATAAATGCATTATGAACTGATGATACTGATTTATTTAATGGATCTCAAAACAAACTTTTAACTTAAGGCACTTTTATTTTGCCAACAAATAACAATAAACAAACATTGAAACGGTTCACTATAAAATAACAAATGGCTAATGTACCTGAATTTTTCAGTAAAAAAATGAACTTCTAATACCAGTTGCCTAGTGTCCACCTCCTATCAATGTTACAAGCATGGCACTCAGAACAGAGACAATGGAAAATATTAAATCTGCAATCTTTATGATGTAAATTTACCATCCTGATGTATAAATATTTTGTGGTTTATAAATTTTTTTGCTAAAACCTACAGAAAAAAANOV29aa, CG55776-03SEQ ID NO:7182591 aaMW at 287114.6 kDProtein SequenceMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNTERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCDAPARTSKGKPLAMVSAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPTPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRIRYSIFRSTTRGSSEKSTTAFSATKLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSISTQTAIPATTPTFPASVITYETQTERSIAAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILEPRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIPNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLELKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVIHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLIRNVRLSDSADFICVARNEGGESVLLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTKTKGISGESLSLHCVSDGPKPNTKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRRITNRPPRSIVTRTGAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29ab, CG55776-04SEQ ID NO:719771 bPDNA SequenceORF Start: at 7ORF Stop: at 766AAGCTTGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTGATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGATGTAATAAAATGCAAAAAAAGATAGAGTCCCTCTAGTGCTCAGCACTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCCTCGAGNOV29ab, CG55776-04SEQ ID NO:720253 aaMW at 29129.5 kDProtein SequenceACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCANOV29ac, CG55776-05SEQ ID NO:7212011 bPDNA SequenceORF Start: at 32ORF Stop: at 1988AGGCTCCGCGGCCGCCCCCTTCACCCTCGAGGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAATTGACCACAAGCAGTATTTTAGAPAAGCAAGTGCTCCATGGGAAGATTTCCAAAGTAGATTGCAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAAGTTTGCCTGAATGGACCATGAATCACATGCAAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAAGCGGAAGGAAGGAGATTATACTTGCTATACCAGAACACCCTAAGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGAACACCAAAATGTACAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAATCCATTTAATGAAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAATAAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAGCAAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTGGACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACAATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCACTCGAGGGCAAGGGTGGGCGCGCCNOV29aC, CG55776-05SEQ ID NO:722652 aaMW at 72283.3 kDProtein SequenceGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKDHKQYFRKQKTLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMVHLTVITAAPRIRQSNKTNKRIKAGDTPAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKAYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVAPNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTANOV29ad, CG55776-06SEQ ID NO:7231975 bPDNA SequenceORF Start: at 11ORF Stop: at 1967CACCAGATCTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAAAGCCTAAGACTGACCTGCCAAAATTGACCACAAGCAGTATTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGAATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCACATGGAACAATTTAATACTTCAACAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGAATCCCAAACCAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACAACATTTCATGCCAATGGGTCTTTGACCATCACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTAATGTGTAGCCCGATCCCAGTGGGGATGACACCAAAAAATGTACAAACTGGATGTGGTCTCTAACCTCCAATTAATCAATGGTCTGTAATAACACAGACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTCCATAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTAATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAUATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTA1KAACACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAAAGATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAATATCAAATGGACTATGCCAAAGTGGTTATGTAGTAGACAGGCCTCAATTAATGGGAAATAAACATATTGCATGACATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAATAGTGTTGGTCATACAACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAGAATTCGGCNOV29ad, CG55776-06SEQ ID NO:724652 aaMW at 72283.3 kDProtein SequenceGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKAGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDWSKPPLINGLYTNRTVIKATAVRHSKKIHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVTQNPQTSDSGIYKCTANOV29ae, CG55776-07SEQ ID NO:7252902 bPDNA SequenceORF Start: at 11ORF Stop: at 2894CACCCTCGAGAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAGAGATCACAGTTCATTCCGGAAGCACTGTGGACTGAAGTGCAGAGCAGAAAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAGACAAAGTGGTGTCTAACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACCAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAAACAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTAACAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29ae, CG55776-07SEQ ID NO:726961 aaMW at 106268.6 kDProtein SequenceNSDAFLPCEAVGNPLPTIHWTRVPSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVTSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCREAGTPSPEVMWIMPDNIFLTAPYYGSRITVHKINGTLEIRNVRLSDSADFICVARNEGGESVLVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29af, CG55776-08SEQ ID NO:7272902 bPDNA SequenceORF Start: at 11ORF Stop: at 2894CACCAGATCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTATAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGAGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAATAGAACTGTTATTAAACATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACGTGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCCTCGAGGGCNOV29af, CG55776-08SEQ ID NO:728961 aaMW at 106268.6 kDProtein SequenceNSDAFLPCEAVGNPLPTIHWTRVPSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCEAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIENLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKDHKQYFRKQKTLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARAPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFANPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29ag, CG55776-09SEQ ID NO:729778 bPDNA SequenceORF Start: at 11ORF Stop: at 770CACCGGATCCGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGATACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTCTTAAAAATGAGCTATAACAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTGATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCCTCGAGGGCNOV29ag, CG55776-09SEQ ID NO:730253 aaMW at 29129.5 kDACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCANOV29ah, SNP13376522 ofSEQ ID NO:7317876 bPCG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 87SNP Change: G to ATCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGACCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCA7AGATGAAAGGACAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAAGAAGGAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAGGCAGATTAAGGAATCAAACATAAAGTTAGTTTACAA3AAAGCACAGCTGTGATGCTTCCTAAACATCTCCTGCTTTACCACAGAGAACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAIAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAGAAGAACAGGAACTGAACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGACTCTTAATCTGACAGATGTGATTGAIAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAACAACTTTGTCCAGCAAATCAACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCAACTAACAACAGTTAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCACCAAACAAAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGAGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29ah, SNP13376522 ofSEQ ID NO:7322617 aaMW at 290230.2 kDCG55776-01, Protein SequenceSNP Pos: 28SNP Change:Ala to ThrMKVKGRGITCLLVSFAVICLVATPGGKTCPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKNSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVEAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNAVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKTQVIAAPPDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29ai, SNP13377624SEQ ID NO:7337876 bPof CG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 113SNP Change: T to CTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTACATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAIAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCAGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAIAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAATAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTTGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29ai, SNP13377624 ofSEQ ID NO:7342617 aaMW at 2902002 kDCG55776-01, Protein SequenceSNP Pos:36SNP Change: Tyr to TyrMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCDAPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLPNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIPNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYThRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29a, SNP13377625SEQ ID NO:7357876 bPof CG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP PoS: 161SNP Change: A to GTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCGGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCACAATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAMATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGATACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAkAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAALAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGAATCATGCCAGACATATTTTCCTCACAGCCCCATACTATGGAAAGCAGATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCATTAIATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAGTAATCTGACATGAATAATAGTCNOV29a, SNP13377625 ofSEQ ID NO:7362617 aaMW at 290200.2 kDCG55776-01, Protein SequenceASNP PoS: 52SNP Change:Pro to ProMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVVSPPPWTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCPAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNTKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29ak, SNP13376523 ofSEQ ID NO:7377876 bPCG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 187SNP Change: G to ATCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACACATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATATGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAACTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAMAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29ak, SNP13376523SEQ ID NO:7382617 aaMW at 290181.1 kDof CG55776-01,SNP Pos: 61SNP Change:Protein SequenceArg to HisMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVEHINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTTIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIPNVRLSDSADFICVARNEGGESVLVVQLEVLEMLARRPTFAPFNEKIVAQLGKSTAIACSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29al, SNP13376524 ofSEQ ID NO:7397876 bPCG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 251SNP Change: A to TTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAATCTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAAACACAAATGGACTATGATTTCAAGGGATACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAGGAGACCCCACCCCACAACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAGCACAGTAAGAGGCACA1ACTATCGGGAAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAATATTTCAGGACTGAAATTTCCCAAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAAACATCCATACCCATGGAAACTCACAAAGTAAACGCCAAGTTACCCAACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAGCATCAATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGACCCAAACAAGAGAGATCTAGAGCACAAAACAATACAAAGAGAACAGGAGCCTCAAAGAAAGAAkCAGGACTGACCCAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAACACAAACTGGGAATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAGCAAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCATAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAAGATGAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAA)GTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATATGATTGTAGCCTACCCTCCCCG1ATTACATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29al, SNP13376524 ofSEQ ID NO:7402617 aaMW at 290186.1 kDCG55776-01, Protein SequenceSNP Pos: 82ISNP Change:LYS to ASnMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTNLELLMLHSNGIHTIPDKTFSDLQALQVRIMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVPAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTNTSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNTNYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPPNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPKTLRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKATKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDENKVHLTVITAAPRIRQSNKTAKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKAGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29am, SNP13376525 ofSEQ ID NO:7417876 bPCG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 493SNP Change: A to GTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCGCTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATCTTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAATGCAA3AAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACkAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAIAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTAACAAAGCATCAATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCAATTCCCCACCAGAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGTTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACAACCCTAAAGGGAAAGATGATGAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAAACCAAAATATTTTCATTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAACTGGATGTGGTCTCTAAACCTCCATTAATCAAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGAATAGCAAGCAATGGTTCTTTTATCATTTCTAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAAGAGCGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAGTGAAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCALACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAAAATCTGACATGAATAATAAAGTCNOV29am, SNP13376525 ofSEQ ID NO:7422617 aaMW at 290219.2 kDCG55776-01, Protein SequenceSNP Pos: 163SNP Change: His to ArgMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTPDKTFSDLQALQVRLMVLKMSYIAKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVRLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVPAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKNLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQEAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSQRVEIQDRGQYLCSASNLFGTDHLHVTLSKTVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29an, SNP13376526SEQ ID NO:7437876 bPof CG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 547SNP Change: G to ATCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAACTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTAAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTACCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29an, SNP13376526 ofSEQ ID NO:7442617 aaMW at 290227.2 kDCG55776-01,SNP Pos: 181SNP Change:Protein SequenceSer to AsnMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSTPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLNYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVAAAKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLIVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29ao, SNP13377626SEQ ID NO:7457876 bPof CG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 4405SNP Change: C to TTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAAAAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTACTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCTCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTQACATGAAATAATAAAGTCNOV29ao, SNP13377626 ofSEQ ID NO:7462617 aaMW at 290216.2 kDCG55776-01, Protein SequenceSNP Pos: 1467SNP Change: Pro to LeuMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFTSPQGFMAPFGSLTLNNTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVLISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRTFRNPFNIEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29ap, SNP13377641SEQ ID NO:7477876 bPof CG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 6716 ISNP Change: C to TTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGTCTTGGTAGGTGGAACCGTTGGCCTGAACTGCCCAGGCCAAGGAGACCCCACCCCACACGTGGATTGGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAGGCCCTTGGAGCATGATGGAGAAACAGAGGGATCTGGACTTGATGAGTCCAATCCTATTGCTCATCTTAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCAGTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCAATTACAAGGTTTTCAAGAAGGAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGCTTCCTAAAACATCTCCTGCTTTACCACAGAGACAAAGTCTCCCCTCGCACCACACTACGACCAAAACACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAAACCACAGCAATTAGTAGCAGAGGTTGCAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACAGCTGTCCTTGACTGTGAGGTCACTGGGGATCCCAAACCAAAAATATTTTGGTTGCTGCCTTCCAATGACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAATAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCATGTGGATCATGCCAGACAATATTTTCCTCACAGCCCCATACTATGGAAGCAGAATCACAGTCCATAAAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAATGAAGGTGGAGAGAGCGTGTTGGTAGTACAGTTAGAAGTACTGGAAATGCTGAGAAGACCGACATTTAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAGTTATCAGTATCTGATAGCAAGCAATGGTTCTTTTATCATTTCTAAAACAACTCGGGAGGATGCAGGAAAATATCGCTGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29ap, SNP13377641 ofSEQ ID NO:7482617 aaMW at 290200.2 kDCG55776-01, Protein SequenceSNP Pos: 2237SNP Change: Asn to AsnMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGTPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRPHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSYIFEKPRIVGGKAASFTIPAIASDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDAGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVINOV29aq, SNP13377640SEQ ID NO:7497876 bPof CG55776-01,ORF Start: ATG at 6ORF Stop: TGA at 7857DNA SequenceSNP Pos: 7218SNP Change: T to CTCAGGATGAAGGTAAAAGGCAGAGGAATCACCTGCTTGCTGGTCTCCTTTGCTGTGATCTGCCTGGTCGCCACCCCTGGGGGCAAGGCCTGTCCTCGCCGCTGTGCCTGTTATATGCCTACGGAGGTACACTGCACATTTCGGTACCTGACTTCCATCCCAGACAGCATCCCGCCCAATGTGGAACGCATCAATTTAGGGTACAACAGCTTGGTTAGATTGATGGAAACAGATTTTTCTGGCCTGACCAAACTGGAGTTACTCATGCTTCACAGCAATGGCATTCACACAATCCCTGACAAGACCTTCTCAGATTTGCAGGCCTTGCAGGTGAGACTGATGGTCTTAAAAATGAGCTATAATAAAGTCCGAAAACTTCAGAAAGATACTTTTTATGGCCTCAGGAGCTTGACACGATTGCACATGGACCACAACAATATTGAGTTTATAAACCCAGAGGTTTTTTATGGGCTCAACTTTCTCCGCCTGGTGCACTTGGAAGGAAATCAGCTCACTAAGCTCCACCCAGATACATTTGTCTCTTTGAGCTACCTCCAGATATTTAAAATCTCTTTCATTAAGTTCCTATACTTGTCTGATAACTTCCTGACCTCCCTCCCTCAAGAGATGGTCTCCTATATGCCTGACCTAGACAGCCTTTACCTGCATGGAAACCCATGGACCTGTGATTGCCATTTAAAGTGGTTGTCTGACTGGATACAGGAGAAGCCAGGTATCTATATTGTNTTACCAGATGTAATAAAATGCAAAAAAGATAGAAGTCCCTCTAGTGCTCAGCAGTGTCCACTTTGCATGAACCCTAGGACTTCTAAAGGCAAGCCGTTAGCTATGGTCTCAGCTGCAGCTTTCCAGTGTGCCAAGCCAACCATTGACTCATCCCTGAAATCAAAGAGCCTGACTATTCTGGAAGACAGTAGTTCTGCTTTCATCTCTCCCCAAGGTTTCATGGCACCCTTTGGCTCCCTCACTTTGAATATGACAGATCAGTCTGGAAATGAAGCTAACATGGTCTGCAGTATTCAAAAGCCCTCAAGGACATCACCCATTGCATTCACTGAAGAAAATGACTACATCGTGCTAAATACTTCATTTTCAACATTTTTGGTGTGCAACATAGATTACGGTCACATTCAGCCAGTGTGGCAAATTTTGGCTTTGTACAGTGATTCTCCTCTGATACTAGAAAGGAGCCACTTGCTTAGTGAAACACCGCAGCTCTATTACAAATATAAACAGGTGGCTCCTAAGCCTGAAGACATTTTTACCAACATAGAGGCAGATCTCAGAGCAGATCCCTCTTGGTTAATGCAAGACCAAATTTCCTTGCAGCTGAACAGAACTGCCACCACATTCAGTACATTACAGATCCAGTACTCCAGTGATGCTCAAATCACTTTACCAAGAGCAGAGATGAGGCCAGTGAAACACAAATGGACTATGATTTCAAGGGATAACAATACTAAGCTGGAACATACTGCTTCTAGCTGATGGAAGTAAAGTGAGAGCCCCTTATGTCAGTGAGGATGGACGGATCCTAATAGACAAAAGTGGAAAATTGGAACTCCAGATGGCTGATAGTTTTGACACAGGCGTATATCACTGTATAAGCAGCAATTATGATGATGCAGATATTCTCACCTATAGGATAACTGTGGTAGAACCTTTGGTCGAAGCCTATCAGGAAAATGGGATTCATCACACAGTTTTCATTGGTGAAACACTTGATCTTCCATGCCATTCTACTGGTATCCCAGATGCCTCTATTAGCTGGGTTATTCCAGGAAACAATGTGCTCTATCAGTCATCAAGAGACAAGAAAGTTCTAAACAATGGCACATTAAGAATATTACAGGTCACCCCGAAAGACCAAGGTTATTATCGCTGTGTGGCAGCCAACCCATCAGGGGTTGATTTTTTGATTTTCCAAGTTTCAGTCAAGATGAAAGGACAAAAAGGAGCCACCAGGTGCACAACTCCGTACATCTGCTCTGATGGAGGCTGAGGTTGGAAAACACACCTCAAGCACAAGTAAGAGGCACAACTATCGGGAATTAACACTCCAGCGACGTGGAGATTCAACACATCGACGTTTTAGGGAGAATAGGAGGCATTTCCCTCCCTCTGCTAGGAGAATTGACCCACAACATTGGGCGGCACTGTTGGAGAAAGCTAAAAAGAATGCTATGCCAGACAAGCGAGAAAATACCACAGTGAGCCCACCCCCAGTGGTCACCCAACTCCCAAACATACCTGGTGAAGAAGACGATTCCTCAGGCATGCTCGCTCTACATGAGGAATTTATGGTCCCGGCCACTAAAGCTTTGAACCTTCCAGCAAGGACAGTGACTGCTGACTCCAGAACAATATCTGATAGTCCTATGACAAACATAAATTATGGCACAGAATTCTCTCCTGTTGTGAATTCACAAATACTACCACCTGAAGAACCCACAGATTTCAAACTGTCTACTGCTATTAAAACTACAGCCATGTCAAAGAATATAAACCCAACCATGTCAAGCCAAATACAAGGCACAACCAATCAACATTCATCCACTGTCTTTCCACTGCTACTTGGAGCAACTGAATTTCAGGACTCTGACCAGATGGGAAGAGGAAGAGAGCATTTCCAAAGTAGACCCCCAATAACAGTAAGGACTATGATCAAAGATGTCAATGTCAAAATGCTTAGTAGCACCACCAACAAACTATTATTAGAGTCAGTAAATACCACAAATAGTCATCAGACATCTGTAAGAGAAGTGAGTGAACCCAGGCACAATCACTTCTATTCTCACACTACTCAAATACTTAGCACCTCCACGTTCCCTTCAGATCCACACACAGCTGCTCATTCTCAGTTTCCGATCCCTAGAAATAGTACAGTTAACATCCCGCTGTTCAGACGCTTTGGGAGGCAGAGGAAAATTGGCGGAAGGGGGCGGATTATCAGCCCATATAGAACTCCAGTTCTGCGACGGCATAGATACAGCATTTTCAGGTCAACAACCAGAGGTTCTTCTGAAAAAAGCACTACTGCATTCTCAGCCACAGTGCTCAATGTGACATGTCTGTCCTGTCTTCCCAGGGAGAGGCTCACCACTGCCACAGCAGCATTGTCTTTTCCAAGTGCTGCTCCCATCACCTTCCCCAAAGCTGACATTGCTAGAGTCCCATCAGAAGAGTCTACAACTCTAGTCCAGAATCCACTATTACTACTTGAGAACAAACCCAGTGTAGAGAAAACAACACCCACAATAAAATATTTCAGGACTGAAATTTCCCAAGTGACTCCAACTGGTGCAGTCATGACATATGCTCCAACATCCATACCCATGGAAAAAACTCACAAAGTAAACGCCACTTACCCACGTGTGTCTAGCACCAATGAAGCTAAAAGAGATTCAGTGATTACATCGTCACTTTCAGGTGCTATCACCAAGCCACCAATGACTATTATAGCCATTACAAGGTTTTCAAGAAGGAAAATTCCCTGGCAACAGAACTTTGTAAATAACCATAACCCAAAAGGCAGATTAAGGAATCAACATAAAGTTAGTTTACAAAAAAGCACAGCTGTGATGACACAATCCTGGAAGTCTTCCAACAAAGAAGGAGCTTCCCTTCCCACCCCTTAACCCTATGCTTCCTAGTATTATAAGCAAAGACTCAAGTACAAAAAGCATCATATCAACGCAAACAGCAATACCAGCAACAACTCCTACCTTCCCTGCATCTGTCATCACTTATGAAACCCAAACAGAGAGATCTAGAGCACAAACAATACAAAGAGAACAGGAGCCTCAAAAGAAGAACAGGACTGACCCAAACATCTCTCCAGACCAGAGTTCTGGCTTCACTACACCCACTGCTATGACACCTCCTGTTCTAACCACAGCCGAAACTTCAGTCAAGCCCAGTGTCTCTGCATTCACTCATTCCCCACCAGAAAACACAACTGGGATTTCAAGCACAATCAGTTTTCATTCAAGAACTCTTAATCTGACAGATGTGATTGAAGAACTAGCCCAAGCAAGTACTCAGACTTTGAAGAGCACAATTGCTTCTGAAACAACTTTGTCCAGCAAATCACACCAGAGTACCACAACTAGGAAAGCAATCATTAGACACTCAACCATACCACCATTCTTGAGCAGCAGTGCTACTCTAATGCCAGTTCCCATCTCCCCTCCCTTTACTCAGAGAGCAGTTACTGACAACGTGGCGACTCCCATTTCCGGGCTTATGACAAATACAGTGGTCAAGCTGCACGAATCCTCAAGGCACAATGCTAACCACAGCAATTAGTAGCAGAGGTTGCAAACATCCCCCAAGGTTCACCCAAATGCCAAGTTCACAATTGGAACCACTCACTTCATCTACTCTAATCTGTTACATTCTACTCCCATGCCAGCACTAACAACAGTTAAATCACAGAATTCTAAATTAACTCCATCTCCCTGGGCAGAAAACCAATTTTGGCACAAACCATACTCAGAAATTGCTGAAAAAGGCAAAAAGCCAGAAGTAAGCATGTTGGCTACTACAGGCCTGTCCGAGGCCACCACTCTTGTTTCAGATTGGGATGGACAGAAGAACACAAAGAAGAGTGACTTTGATAAGAAACCAGTTCAAGAAGCAACAACTTCCAAACTCCTTCCCTTTGACTCTTTGTCTAGGTATATATTTGAAAAGCCCAGGATAGTTGGAGGAAAAGCTGCAAGTTTTACTATTCCAGCTAACTCAGATGCCTTTCTTCCCTGTGAAGCTGTTGGAAATCCCCTGCCCACCATTCATTGGACCAGAGTCCCATCAGGTATGTCAGGACTTGATTTATCTAAGAGGAAACAGAATAGCAGGGTCCAGGTTCTCCCCAATGGTACCCTGTCCATCCAGAGGGTGGAAATTCAGGACCGCGGACAGTACTTGTGTTCCGCATCCAATCTGTTTGGCACAGACCACCTTCATGTCACCTTGTCTGTGGTTTCCTATCCTCCCAGGATCCTGGAGAGACGTACCAAAGAGATCACAGTTCATTCCGGAAGCACTGTGGAACTGAAGTGCAGAGCAGAAGGTAGGCCAAGCCCTACAGTTACCTGGATTCTTGCAAACCAAACAGTTGTCTCAGAATCATCCCAGGGAAGTAGGCAGGCTGTGGTGACGGTTGACGGAACATTGGTCCTCCACAATCTCAGTATTTATGACCGTGGCTTTTACAAATGTGTGGCCAGCAACCCAGGTGGCCAGGATTCACTGCTGGTTAAAATACAAGTCATTGCAGCACCACCTGTTATTCTAGAGCAAAGGAGGCAAGTCATTGTAGGCACTTGGGGTGAAAGTTTAAAACTGCCCTGTACTGCAAAAGGAACTCCTCAGCCCAGCGTTTACTGGGTCCTCTCTGATGGCACTGAAGTGAAACCATTACAGTTTACCAATTCCAAGTTGTTCTTATTTTCAAATGGGACTTTGTATATAAGAAACCTAGCCTCTTCAGACAGGGGCACTTATGAATGCATTGCTACCAGTTCCACTGGTTCGGAGCGAAGAGTAGTAATGCTTACAATGGAAGAGCGAGTGACCAGCCCCAGGATAGAAGCTGCATCCCAGAAAAGGACTGAAGTGAATTTTGGGGACAAATTACTACTGAACTGCTCAGCCACTGGGGAGCCCAAACCCCAAATAATGTGGAGGTTACCATCCAAGGCTGTGGTCGACCAGCAGCATAGGGTGGGCAGCTGGATCCACGTCTACCCTAATGGATCCCTGTTTATTGGATCAGTAACAGAAAAAGACAGTGGTGTCTACTTGTGTGTGGCAAGAAACAAAATGGGGGATGATCTGATACTGATGCATGTTAGCCTAAGACTGAAACCTGCCAAAATTGACCACAAGCAGTATTTTAGAAAGCAAGTGCTCCATGGGAAAGATTTCCAAGTAGATTGCAAAGCTTCCGGCTCCCCAGTGCCAGAGATATCTTGGAGTTTGCCTGATGGAACCATGATCAACAATGCAATGCAAGCCGATGACAGTGGCCACAGGACTAGGAGATATACCCTTTTCAACAATGGAACTTTATACTTCAACAAAGTTGGGGTAGCGGAGGAAGGAGATTATACTTGCTATGCCCAGAACACCCTAGGGAAAGATGAAATGAAGGTCCACTTAACAGTTATAACAGCTGCTCCCCGGATAAGGCAGAGTAACAAAACCAACAAGAGAATCAAAGCTGGAGACACACATGATTTCCTTCTCCATTGATAGGTACACATTTCATGCCAATGGGTCTTTGACCATCAACAAAGTGAAACTGCTCGATTCTGGAGAGTACGTATGTGTAGCCCGAAATCCCAGTGGGGATGACACCAAAATGTACAAACTGGATGTGGTCTCTAAACCTCCATTAATCAATGGTCTGTATACAAACAGAACTGTTATTAAAGCCACAGCTGTGAGACATTCCAAAAAACACTTTGACTGCAGAGCTGAAGGGACACCATCTCCTGAAGTCAAATGGAACCTTGGAAATTAGGAATGTGAGGCTTTCAGATTCAGCCGACTTTATCTGTGTGGCCCGAAAGAAATCCATTTAATGAAAAAATAGTTGCCCAGCTGGGAAAGTCCACAGCATTGAATTGCTCTGTTGATGGTAACCCACCACCTGAAATAATCTGGATTTTACCAAATGGCACACGATTTTCCAATGGACCACAAAAAATATCGCCGTGCAGCTAGGAATAAAGTTGGCTATATTGAGAAATTAGTCATATTAGAAATTGGCCAGAAGCCAGTTATTCTTACCTATGCACCAGGGACAGTAAAAGGCATCAGTGGAGAATCTCTATCACTGCATTGTGTGTCTGATGGAATCCCTAAGCCAAATATCAAATGGACTATGCCAAGTGGTTATGTAGTAGACAGGCCTCAAATTAATGGGAAATACATATTGCATGACAATGGCACCTTAGTCATTAAAGAAGCAACAGCTTATGACAGAGGAAACTATATCTGTAAGGCTCAAAATAGTGTTGGTCATACACTGATTACTGTTCCAGTAATGATTGTAGCCTACCCTCCCCGAATTACAAATCGTCCACCCAGGAGTATTGTCACCAGGACAGGGGCAGCCTTTCAGCTCCACTGTGTGGCCTTGGGAGTTCCCAAGCCAGAAATCACATGGGAGATGCCTGACCACTCCCTTCTCTCAACGGCAAGTAAAGAGAGGACACATGGAAGTGAGCAGCTTCACTTACAAGGTACCCTAGTCATTCAGAATCCCCAAACCTCCGATTCTGGGATATACAAATGCACAGCAAAGAACCCACTTGGTAGTGATTATGCAGCAACGTATATTCAAGTAATCTGACATGAAATAATAAAGTCNOV29aq, SNP13377640 ofSEQ ID NO:7502617 aaMW at 290253.2 kDCG55776-01, Protein SequenceSNP Pos: 2405SNP Change: Cys to ArgMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFLYLSDNFLTSLPQEMKTSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPGIYIVLPDVIKCKKDRSPSSAQQCPLCAPRTSKGKPLAMVSAAAFQCAKPTIDSSLKSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPEAEMRPVKHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPNVDWLLADGSKVRAPYVSEDGRILIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGETLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEATTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTAKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSERRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYKLDVVSKPPLINGLYTNRTVIKATAVRHSKKHFDCRAEGTPSPEVMWIMPDNIFLTAPYYGSRITVHKNGTLEIRNVRLSDSADFICVARNEQYLIASNGSFIISKTTREDAGKYPRAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVI


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 29B.

TABLE 29BComparison of the NOV29 protein sequences.NOV29aMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVENOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVENOV29aaMKVKGRGITCLLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVENOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aRINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zRINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKNOV29aaRINLGYNSLVRLMETDFSGLTKLELLMLHSNGIHTIPDKTFSDLQALQVRLMVLKMSYNKNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aVRKLQKDTFYGLRSLTRLHNDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLNOV29aaVRKLQKDTFYGLRSLTRLHMDHNNIEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQENOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQENOV29aaSYLQIFKISFIKFLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQENOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKNOV29aaKPGIYIVLPDVIKCKKDRSPSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCAKPTIDSSLKNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENNOV29aaSKSLTILEDSSSAFISPQGFMAPFGSLTLNMTDQSGNEANMVCSIQKPSRTSPIAFTEENNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aDYIVLNTSFSTFLVCNIDYGHIQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zDYIVLNTSFSTFLVCNIDYGHTQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPNOV29aaDYIVLNTSFSTFLVCNIDYGHTQPVWQILALYSDSPLILERSHLLSETPQLYYKYKQVAPNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPRAEMRPVKNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPPAENRPVKNOV29aaKPEDIFTNIEADLRADPSWLMQDQISLQLNRTATTFSTLQIQYSSDAQITLPEAENRPVKNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILNOV29aaHKWTMISRDNNTKLEHTVLVGGTVGLNCPGQGDPTPHVDWLLADGSKVRAPYVSEDGRILNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGENOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGENOV29aaIDKSGKLELQMADSFDTGVYHCISSNYDDADILTYRITVVEPLVEAYQENGIHHTVFIGENOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aTLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zTLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANNOV29aaTLDLPCHSTGIPDASISWVIPGNNVLYQSSRDKKVLNNGTLRILQVTPKDQGYYRCVAANNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGNOV29aaPSGVDFLIFQVSVKMKGQRPLEHDGETEGSGLDESNPIAHLKEPPGAQLRTSALMEAEVGNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPNOV29aaKHTSSTSKRHNYRELTLQRRGDSTHRRFRENRRHFPPSARRIDPQHWAALLEKAKKNAMPNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISNOV29aaDKRENTTVSPPPVVTQLPNIPGEEDDSSGMLALHEEFMVPATKALNLPARTVTADSRTISNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHNOV29aaDSPMTNINYGTEFSPVVNSQILPPEEPTDFKLSTAIKTTAMSKNINPTMSSQIQGTTNQHNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKMLSSTTNKLLLESVNNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKNLSSTTNKLLLESVNNOV29aaSSTVFPLLLGATEFQDSDQMGRGREHFQSRPPITVRTMIKDVNVKNLSSTTNKLLLESVNNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRNOV29b------------------------------------------------------------KOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zTTNSHQTSVREVSEPRHNHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRNOV29aaTTNSHQTSVREVSEPRENHFYSHTTQILSTSTFPSDPHTAAHSQFPIPRNSTVNIPLFRRNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zFGRQRKIGGRGRIISPYRTPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRNOV29aaFGRQRKIGGRGRIISPYRYPVLRRHRYSIFRSTTRGSSEKSTTAFSATVLNVTCLSCLPRNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFNOV29aaERLTTATAALSFPSAAPITFPKADIARVPSEESTTLVQNPLLLLENKPSVEKTTPTIKYFNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPNOV29aaRTEISQVTPTGAVMTYAPTSIPMEKTHKVNASYPRVSSTNEAKRDSVITSSLSGAITKPPNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPNOV29aaMTIIAITRFSRRKIPWQQNFVNNHNPKGRLRNQHKVSLQKSTAVMLPKTSPALPQRQSLPNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVNOV29aaSHHTTTKTHNPGSLPTKKELPFPPLNPMLPSIISKDSSTKSIISTQTAIPATTPTFPASVNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zITYETQTERSEAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSNOV29aaITYETQTERSRAQTIQREQEPQKKNRTDPNISPDQSSGFTTPTAMTPPVLTTAETSVKPSNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSNOV29aaVSAFTHSPPENTTGISSTISFHSRTLNLTDVIEELAQASTQTLKSTIASETTLSSKSHQSNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQPAVTDNVATPISGLMTNTVVKLHESSNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSNOV29aaTTTRKAIIRHSTIPPFLSSSATLMPVPISPPFTQRAVTDNVATPISGLMTNTVVKLHESSNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aRHNAKPQQLVAEVATSFKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zRHNAKPQQLVAEVATSPKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPNOV29aaRHNAKPQQLVAEVATSFKVHPNAKFTIGTTHFIYSNLLHSTPMPALTTVKSQNSKLTPSPNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEANOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------------------------------------NOV29y------------------------------------------------------------NOV29zWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEANOV29aaWAENQFWHKPYSEIAEKGKKPEVSMLATTGLSEATTLVSDWDGQKNTKKSDFDKKPVQEANOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae------------------------------------------------------------NOV29af------------------------------------------------------------NOV29ag------------------------------------------------------------NOV29aTTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x------------------------------TLENSDAFLPCEAVGNPLPTIHWTRVPS--NOV29y------------------------------TRSNSDAFLPCEAVGNPLPTIHWTRVPS--NOV29zTTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMNOV29aaTTSKLLPFDSLSRYIFEKPRIVGGKAASFTIPANSDAFLPCEAVGNPLPTIHWTRVPSGMNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae---------------------------------NSDAFLPCEAVGNPLPTIHWTRVPS--NOV29af---------------------------------NSDAFLPCEAVGNPLPTIHWTRVPS--NOV29ag------------------------------------------------------------NOV29aSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29x-GLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29y-GLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29zSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29aaSGLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29ae-GLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29af-GLDLSKRKQNSRVQVLPNGTLSIQRVEIQDRGQYLCSASNLFGTDHLHVTLSVVSYPPRNOV29ag------------------------------------------------------------NOV29aILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29xILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29yILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29zILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29aaILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29aeILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29afILERRTKEITVHSGSTVELKCRAEGRPSPTVTWILANQTVVSESSQGSRQAVVTVDGTLVNOV29ag------------------------------------------------------------NOV29aLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTANOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29xLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTANOV29yLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTANOV29zLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTANOV29aaLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQPRQVIVGTWGESLKLPCTANOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29aeLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTANOV29afLHNLSIYDRGFYKCVASNPGGQDSLLVKIQVIAAPPVILEQRRQVIVGTWGESLKLPCTANOV29ag------------------------------------------------------------NOV29aKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29b------------------------------------------------------------NOV29c------------------------------------------------------------NOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h------------------------------------------------------------NOV29i------------------------------------------------------------NOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w------------------------------------------------------------NOV29xKGTPQPSVYWVLSDGTEVKFLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29yKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29zKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29aaKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29ab------------------------------------------------------------NOV29ac------------------------------------------------------------NOV29ad------------------------------------------------------------NOV29aeKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29afKGTPQPSVYWVLSDGTEVKPLQFTNSKLFLFSNGTLYIRNLASSDRGTYECIATSSTGSENOV29ag------------------------------------------------------------NOV29aRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29b------------------------------------------------------------NOV29c-------------------GSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29h-------------------GSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29i-------------------GSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29w-------------------GSAAAPFTLEGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29xRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29yRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQTMWRLPSKAVVDQQNOV29zRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQTMWRLPSKAVVDQQNOV29aaRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQTMWRLPSKAVVDQQNOV29ab------------------------------------------------------------NOV29ac-----------------------------GDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29ad-----------------------------GDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29aeRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29afRRVVMLTMEERVTSPRIEAASQKRTEVNFGDKLLLNCSATGEPKPQIMWRLPSKAVVDQQNOV29ag------------------------------------------------------------NOV29aHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29b------------------------------------------------------------NOV29cHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29hHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29iHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29wHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29xHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29yHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29zHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29aaHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLELKPAKIDHKQYFNOV29ab------------------------------------------------------------NOV29acHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29adHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29aeHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29afHRVGSWIHVYPNGSLFIGSVTEKDSGVYLCVARNKMGDDLILMHVSLRLKPAKIDHKQYFNOV29ag------------------------------------------------------------NOV29aRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29b------------------------------------------------------------NOV29cRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29hRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29iRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29j------------------------------------------------------------NOV29k------------------------------------------------------------NOV29l------------------------------------------------------------NOV29m------------------------------------------------------------NOV29n------------------------------------------------------------NOV29o------------------------------------------------------------NOV29p------------------------------------------------------------NOV29q------------------------------------------------------------NOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29wRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29xRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29yRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29zRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29aaRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29ab------------------------------------------------------------NOV29acRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29adRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29aeRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29afRKQVLHGKDFQVDCKASGSPVPEISWSLPDGTMINNAMQADDSGHRTRRYTLFNNGTLYFNOV29ag------------------------------------------------------------NOV29aNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29b------------------------------------------------------------NOV29cNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29hNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29iNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29j----------------------------------------------TLEGDKLLLNCSATNOV29k----------------------------------------------TLEGDKLLLNCSATNOV29l----------------------------------------------TLEGDKLLLNCSATNOV29m----------------------------------------------TLEGDKLLLNCSATNOV29n----------------------------------------------TLEGDKLLLNCSATNOV29o----------------------------------------------TLEGDKLLLNCSATNOV29p----------------------------------------------TLEGDKLLLNCSATNOV29q----------------------------------------------TLEGDKLLLNCSATNOV29r-------------------------------------------------------APRPPNOV29s----------------------------------------------TLEMKVKGRGITCLNOV29t----------------------------------------------TLEMKVKGRGITCLNOV29u----------------------------------------------TLEMKVKGRGITCLNOV29v----------------------------------------------TLEMKVKGRGITCLNOV29wNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29xNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29yNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29zNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDREVTNOV29aaNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29ab------------------------------------------------------------NOV29acNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29adNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29aeNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29afNKVGVAEEGDYTCYAQNTLGKDEMKVHLTVITAAPRIRQSNKTNKRIKAGDTAVLDCEVTNOV29ag------------------------------------------------------------NOV29aGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29b-------------------------------------------TLENVERINLGYNSLVRNOV29cGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29d--------------KLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29e--------------KLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29f--------------KLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29g--------------KLACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29hGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29iGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29jGEPKPQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29kGEPKPQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29lGEPKPQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29mGEPKPQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29nGEPKPQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29oGEPKPQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29pGEPKPQIMWRLQS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29qGEPKHQIMWRLPS-KAVVDQQHRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29rPSPSSVLAAVPVICTLECPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29sLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29tLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29uLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29vLVSFAVICLVATPGGKACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29wGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29xGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29yGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29zGDPKPKIFWLLPS---------------NGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29aa--------------------------HANGSLTINKVKLLDSGEYVCVARNPSGDDTKMYNOV29ab----------------ACPRRCACYMPTEVHCTFRYLTSIPDSIPPNVERINLGYNSLVRNOV29acGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKIYNOV29adGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKIYNOV29aeGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKIYNOV29afGDPKPKIFWLLPSNDMISFSIDRYTFHANGSLTINKVKLLDSGEYVCVARNPSGDDTKIYNOV29ag----------------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--IEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29cGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29dTRLHMDHNN--IEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29eTRLHMDHNN--IEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29fTRLHMDHNN--IEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29gTRLHMDHNN--IEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29hGSRITVHKNGTLEIRNRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29iGSRITVHKNGTLEIRNRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29jGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29kGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29lGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29mGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29nGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29oGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29pGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPAFRNPFNEKNOV29qGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29rTRLHMDHNN--IEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29sTRLHMDHNN--IEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29tTRLHMDHNN--IEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29uTRLHMDHNN--IEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29vTRLHMDHNN--IEFINPEVFYGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29wGSRITVHKNGTLEIRYVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29xGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29yGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29zGGRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29aaGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29abTRLHMDHNN--IEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29acGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29adGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29aeGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29afGSRITVHKNGTLEIRNVRLSDSADFICVARNEGGESVLVVQLEVLEMLRRPTFRNPFNEKNOV29agTRLHMDHNN--IEFINPEVFDGLNFLRLVHLEGNQLTKLHPDTFVSLSYLQIFKISFIKFNOV29aIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29bLYLSDNFLTSLPQEMVSYMP------LEG-------------------------------NOV29cIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29dLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPNOV29eLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPNOV29fLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPNOV29gLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLFDWIQEKPDVIKCKKDRSPNOV29hIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29iIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29jIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29kIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29lIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29mIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29nIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29oIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29pIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29qIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29rLYLSDNFLTSLPQEMVSYMP------LEGKGGRA--------------------------NOV29sLYLSDNFLTSLPQEMVSYMP------LEG-------------------------------NOV29tLYLSDNFLTSLPQEMVSYMP------LEG-------------------------------NOV29uLYLSDNFLTSLPQEMVSYMP------LEG-------------------------------NOV29vLYLSDNFLTSLPQEMVSYMP------LEG-------------------------------NOV29wIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29xIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29yIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29zIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29aaIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29abLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPNOV29acIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29adIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29aeIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29afIVAQLGKSTALNCSVDGNPPPEIIWILPNGTRFSNGPQSYQYLIASNGSFIISKTTREDANOV29agLYLSDNFLTSLPQEMVSYMPDLDSLYLHGNPWTCDCHLKWLSDWIQEKPDVIKCKKDRSPNOV29aGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29b------------------------------------------------------------NOV29cGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29dSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCALE---------------------------NOV29eSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCALE---------------------------NOV29fSSAPQCPLCNNPRTSKGKPLAMVSAAAFQCALE---------------------------NOV29gSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCALE---------------------------NOV29hGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29iGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29jGKNRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29kGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29lGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29mGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29nGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29oGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29pGKYRCAAPNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29qGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29wGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29xGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29yGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29zGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29aaGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29abSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCA-----------------------------NOV29acGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29adGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29aeGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29afGKYRCAARNKVGYIEKLVILEIGQKPVILTYAPGTVKGISGESLSLHCVSDGIPKPNIKWNOV29agSSAQQCPLCMNPRTSKGKPLAMVSAAAFQCA-----------------------------NOV29aTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29b------------------------------------------------------------NOV29cTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29hTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29iTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29jTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29kTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29lTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29mTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29nTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29oTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29pTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29qTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29wTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29xTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29yTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29zTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29aaTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29ab------------------------------------------------------------NOV29acTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29adTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29aeTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29afTMPSGYVVDRPQINGKYILHDNGTLVIKEATAYDRGNYICKAQNSVGHTLITVPVMIVAYNOV29ag------------------------------------------------------------NOV29aPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29b------------------------------------------------------------NOV29cPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29d------------------------------------------------------------NOV29e------------------------------------------------------------NOV29f------------------------------------------------------------NOV29g------------------------------------------------------------NOV29hPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29iPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29jPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29kPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHRSEQLHLNOV29lPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29mPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29nPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29oPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29pPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29qPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMSDHSLLSTASKERTHGSEQLHLNOV29r------------------------------------------------------------NOV29s------------------------------------------------------------NOV29t------------------------------------------------------------NOV29u------------------------------------------------------------NOV29v------------------------------------------------------------NOV29wPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29xPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29yPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29zPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29aaPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29ab------------------------------------------------------------NOV29acPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29adPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29aePPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29afPPRITNRPPRSIVTRTGAAFQLHCVALGVPKPEITWEMPDHSLLSTASKERTHGSEQLHLNOV29ag------------------------------------------------------------NOV29aQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVI--------NOV29b---------------------------------------------NOV29cQGTLVIQNPQTSDSGIYKCTALE----------------GKGGRANOV29d---------------------------------------------NOV29e---------------------------------------------NOV29f---------------------------------------------NOV29g---------------------------------------------NOV29hQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEGKGGRANOV29iQGTLVIQNPQTSDSGIYKCTALE----------------GKGGRANOV29jQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29kQGTLVIQNPQTSDSGIYKCTALEG---------------------NOV29lQGTLVIQNPQTSDSGIYKCTALEGKGE------------------NOV29mQGTLVIQNPQTSDSGIYKCTALEG---------------------NOV29nQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29oQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29pQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29qQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29r---------------------------------------------NOV29s---------------------------------------------NOV29t---------------------------------------------NOV29u---------------------------------------------NOV29v---------------------------------------------NOV29wQGTLVIQNPQTSDSGIYKCTALE----------------GKGGRANOV29xQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29yQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVILEG-----NOV29zQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVI--------NOV29aaQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVI--------NOV29ab---------------------------------------------NOV29acQGTLVIQNPQTSDSGIYKCTA------------------------NOV29adQGTLVIQNPQTSDSGIYKCTA------------------------NOV29aeQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVI--------NOV29afQGTLVIQNPQTSDSGIYKCTAKNPLGSDYAATYIQVI--------NOV29ag---------------------------------------------NOV29a(SEQ ID NO: 666)NOV29b(SEQ ID NO: 668)NOV29c(SEQ ID NO: 670)NOV29d(SEQ ID NO: 672)NOV29e(SEQ ID NO: 674)NOV29f(SEQ ID NO: 676)NOV29g(SEQ ID NO: 678)NOV29h(SEQ ID NO: 680)NOV29i(SEQ ID NO: 682)NOV29j(SEQ ID NO: 684)NOV29k(SEQ ID NO: 686)NOV29l(SEQ ID NO: 688)NOV29m(SEQ ID NO: 690)NOV29n(SEQ ID NO: 692)NOV29o(SEQ ID NO: 694)NOV29p(SEQ ID NO: 696)NOV29q(SEQ ID NO: 698)NOV29r(SEQ ID NO: 700)NOV29s(SEQ ID NO: 702)NOV29t(SEQ ID NO: 704)NOV29u(SEQ ID NO: 706)NOV29v(SEQ ID NO: 708)NOV29w(SEQ ID NO: 710)NOV29x(SEQ ID NO: 712)NOV29y(SEQ ID NO: 714)NOV29z(SEQ ID NO: 716)NOV29aa(SEQ ID NO: 718)NOV29ab(SEQ ID NO: 720)NOV29ac(SEQ ID NO: 722)NOV29ad(SEQ ID NO: 724)NOV29ae(SEQ ID NO: 726)NOV29af(SEQ ID NO: 728)NOV29ag(SEQ ID NO: 730)


Further analysis of the NOV29a protein yielded the following properties shown in Table 29C.

TABLE 29CProtein Sequence Properties NOV29aSignalP analysis:Cleavage site between residues 29 and 30PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos. chg 3; neg. chg 0H-region:length 20; peak value 10.48PSG score:6.08GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −1.23possible cleavage site: between 28 and 29>>> Seems to have a cleavable signal peptide (1 to 28)ALOM:Klein et al's method for TM region allocationInit position for calculation: 29Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 0PERIPHERALLikelihood = 1.91 (at 1062)ALOM score: 0.37 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 14Charge difference: −2.0 C(2.0)-N(4.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:3Hyd Moment(75):5.15Hyd Moment(95):8.32G content:4D/E content:1S/T content:4Score: −2.83Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 56 FRY|LTNUCDISC: discrimination of nuclear localization signalspat4: RRHR (3) at 1043pat7: PVLRRHR (3) at 1040pat7: PQKKNRT (4) at 1341bipartite: RRIDPQHWAALLEKAKK at 760content of basic residues: 11.1%NLS Score: 0.81KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: foundILPN at 2366RNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):73.9%: nuclear 8.7%: mitochondrial 8.7%: extracellular, including cell wall 4.3%: cytoplasmic 4.3%: vacuolar>> prediction for CG55776-01 is nuc (k = 23)


A search of the NOV29a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 29D.

TABLE 29DGeneseq Results for NOV29aNOV29aIdentities/Residues/SimilaritiesGeneseqProtein/Organism/LengthMatchfor theExpectIdentifier[Patent #, Date]ResiduesMatched RegionValueABG31323Human OCP protein - Homo sapiens,1 . . . 26172535/2637 (96%)0.02589 aa. [WO200246364-A2,1 . . . 25892541/2637 (96%)13-JUN-2002]AAB47935Human OCP - Homo sapiens,1 . . . 26172534/2637 (96%)0.02587 aa. [US2002022026-A1,1 . . . 25872540/2637 (96%)21-FEB-2002]ABG32896Human osteoclast protein (OCP) #2 -1 . . . 26172534/2637 (96%)0.0Homo sapiens, 2587 aa.1 . . . 25872540/2637 (96%)[US2002086825-A1, 04-JUL-2002]ABG32891Human osteoclast protein (OCP) #1 -1 . . . 26162533/2636 (96%)0.0Homo sapiens, 2586 aa.1 . . . 25862539/2636 (96%)[US2002086825-A1, 04-JUL-2002]ABG31317Human 5 + 3 corrected OCP protein -1 . . . 26162526/2636 (95%)0.0Homo sapiens, 2586 aa.1 . . . 25862534/2636 (95%)[WO200246364-A2, 13-JUN-2002]


In a BLAST search of public sequence databases, the NOV29a protein was found to have homology to the proteins shown in the BLASTP data in Table 29E.

TABLE 29EPublic BLASTP Results for NOV29aNOV29aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8NA84Hypothetical protein FLJ35750 - Homo1982 . . . 2617636/636(100%)0.0sapiens (Human), 650 aa. 15 . . . 650636/636(100%)AAH31063Similar to hypothetical protein2016 . . . 2617601/602(99%)0.09030224D03 - Homo sapiens (Human), 1 . . . 602602/602(99%)602 aa.Q9NR99Adlican - Homo sapiens (Human), 28281637 . . . 2616451/980(46%)0.0aa.1851 . . . 2827640/980(65%)BAC28314Adult male colon cDNA, RIKEN full-length2079 . . . 2617419/539(77%)0.0enriched library, clone: 9030224D03 1 . . . 539479/539(88%)product: hypothetical Immunoglobulin andmajor histocompatibility complexdomain/Immunoglobulin C-2type/Immunoglobulin-like/Immunoglobulinsubtype containing protein, full insertsequence - Mus musculus (Mouse), 539aa (fragment).Q8N772Hypothetical protein FLJ25972 - Homo2219 . . . 2617399/399(100%)0.0sapiens (Human), 399 aa. 1 . . . 399399/399(100%)


PFam analysis predicts that the NOV29a protein contains the domains shown in the Table 29F.

TABLE 29FDomain Analysis of NOV29aIdentities/SimilaritiesPfamNOV29afor theExpectDomainMatch RegionMatched RegionValueLRRNT28 . . . 5612/35 (34%)0.0001523/35 (66%)LRR 82 . . . 105 8/25 (32%)0.2519/25 (76%)LRR134 . . . 157 8/25 (32%)0.08821/25 (84%)LRR158 . . . 18112/25 (48%)0.3219/25 (76%)LRRCT223 . . . 29117/69 (25%)  8e−1052/69 (75%)ig501 . . . 56416/67 (24%)0.5940/67 (60%)ig599 . . . 65818/63 (29%)1.9e−0744/63 (70%)ig1654 . . . 171914/69 (20%)0.00246/69 (67%)ig1754 . . . 181617/66 (26%)5.7e−0545/66 (68%)ig1851 . . . 191319/66 (29%)  1e−0747/66 (71%)ig1950 . . . 201221/66 (32%)5.1e−0846/66 (70%)ig2047 . . . 211513/72 (18%)0.000244/72 (61%)ig2150 . . . 220920/63 (32%)  6e−0945/63 (71%)ig2248 . . . 230913/65 (20%)0.3638/65 (58%)ig2346 . . . 240721/65 (32%)1.3e−0644/65 (68%)ig2441 . . . 250216/65 (25%)0.02241/65 (63%)ig2537 . . . 260116/68 (24%)1.1e−0546/68 (68%)


Example 30

The NOV30 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 30A.

TABLE 30ANOV30 Sequence AnalysisNOV30a, CG55784-03SEQ ID NO: 7513772 bpDNA SequenceORF Start: ATG at 251ORF Stop: TAA at 2549CTTCTCCGAGTGGGGACATTGCTGACAATCCCGGCTTCCCGAGGCGGCTAAGAACAGGCAGTTTGTGTCGGCTGGCTGCAGATACCCAGAGGCACAAAGAGACCGAAGCCACCCGGAGGGACCCACGGACGGACAGATGGTAGGCGCGAACCCGAGAGGACCGGCGGAGGCTGAGCACCGAGAGCCGCCAAGGAAGAGAAACTAACCACAGCCAAGTTACCCCGCCGGCTTTCCTTCGCTGCGCTAAGGAATGAAACCCTTCCAGCTCGATCTGCTCTTCGTCTGCTTCTTCCTCTTCAGTCAAGAGCTGGGCCTCCAGAAGAGAGGATGCTGTCTGGTGCTGGGCTACATGGCCAAGGACAAGTTTCGGAGAATGAATGAAGGCCAAGTCTATTCCTTCAGCCAGCAGCCCCAGGACCAGGTGGTGGTGTCGGGACAGCCAGTGACGCTACTTTGCGCCATCCCCGAATACGATGTACCTGGTGGTAGGGAACCACCTGTCAGGGGAGCACCACCTGAAGATCCTGAGGGCAGAGCTGCAAGACGATGCGGTGTACGAGTGCCAGGCCATCCAGGCCGCCATCCGCTCCCGCCCCGCACGCCTCACAGTCCTGGTGCCGCCTGATGACCCCGTCATCCTGGGGGGCCCTGTGATCAGCCTGCGTGCGGGGGACCCTCTCAACCTCACCTGCCACGCAGACAATGCCAAGCCTGCAGCCTCCATCATCTGGTTGCGAAAGGGAGAGGTCATCAATGGGGCCACCTACTCCAAGACCCTGCTTCGGGACGGCAAGCGGGAGAGCATCGTCAGCACCCTCTTCATCTCCCCTGGTGACGTGGAGAATGGCCAGAGCATCGTGTGTCGTGCCACCAACAAAGCCATCGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAACGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTCAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCGCCTCTGGTCGGGAGGGTGAGGAGCACTCCACCATCAAGCAGCTGATGATGGACCGGGGTGAATTCCAGCAAGACTCAGTCCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAAGAGTTTCAGAACCTGAAGGACCCCACCAATGGCTACTACAGCGTCAACACCTTCAAAGAGCACCACTCAACCCCGACCATCTCCCTCTCCAGCTGCCAGCCCGACCTGCGTCCTGCGGGTAAGCAGCGTGTGCCCACAGGCATGTCCTTCACCAACATCTACAGCACCCTGAGCGGCCAGGGCCGCCTCTACGACTACGGGCAGCGGTTTGTGCTGGGCATGGGCAGCTCGTCCATCGAGCTTTGTGAGCGGGAGTTCCAGAGAGGCTCCCTCAGCGACAGCAGCTCCTTCCTGGACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAGGATGGCTATGTGCAGTTCGACAAGACCCCTGCAGCGGCGGATGCAGACTCACGTCTAAGGATCACACACCGCGGGTGGGGACGGGCCAGGCAAGAGGTCAGGGCACGTTCTGGTTGTCCAGGGACGAGGGGTACTTTGCAGAGGACACCAGAATTGGCCACTTCCAGGACAGCCTCCCAGCGCCTCTGCCACTGCCTTCCTTCGAAGCTCTGATCAAGCACAAATCTGGGTCCCCAGGTGCTGTGTGCCAGAGGTGGGCGGGTGGGGAGACAGACAGAGGCTGCGGCTGAGTGCGCTGTGCTTAGTGCTGGACACCCGTGTCCCCGGCCCTTTCCTGGAGGCCCCTCTACCACCTGCTCTGCCCACAGGCACAAGTGGCAGCTATAACTCTGCTTTCATGAAACTGCGGTCCACTCTCTGGTCTCTCTGTGGGCTCTACCCCTCACTGACCACAAGCTCTACCTACCCCTGTGCCTGTGCTCCCATACAGCCCTGGGGGCCATACCCTGGAGGTTGACAAGCCAGAAATGGCCTTGGCTAAAGGAGCCTCTCTCTCACCAGGCTGGCCGGGAGCCCACCCCCAATTTGTTTGGTGTTTTGTGTCCATACTCTTGCAGTTCTGTCCTTGGACTTGATGCCGCTGAACTCTGCGGTGGGACCGGTCCCGTCAGAGCCTGGTGTACTGGGGGGAGGGAGGGAGGAGGGAGCCTGTGCTGACGGAGCACCTCGCCGGGTGTGCCCCTCCTGGGCTGTGTGACCCCAGCCTCCCCACCCACCTCCTGCTTTGTGTACTCCTCCCCTCCCCCTCAGCACAATCGGAGTTCATATAAGAAGTGCGCGAGCTTCTCTGGTCAGGGTTCTCTGAACACTTATGGAGAGAGTGCTTCCTGGGAAGTGTGGCGTTTGAAGGGGCTGGAGGGCAGGTCTTTAAGATGGCGAGACTGCCCTTCTCAGCTGATAAACACAAGAACGGCCATCCTGTCTTCAGTAAGGCTCCACGAGAAGAGAGGAAGTATATCTACACCTCAACCCTCCTAGTCACCACCTGAAATAATGTTAGGGACACTACTCCAACATGTTTGTTCTGTTCTTTTGTTCCTACAAAAGCCACAGGAAGAACCCAAGAGCTCATAGAATGCGTTGGGAACCCAAGGTTCTCTGCCCTCCTTTGATTCAATCATCCTAGACAATAAAGGCAGTTGATAGCTCTGNOV30a, CG55784-03Protein SequenceSEQ ID NO: 752766 aaMW at 84135.5kDMKPFQLDLLFVCFFLFSQELGLQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRPARLTVLVPPDDPVILGGPVISLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHVNOV30b, CG55784-01SEQ ID NO: 7531806 bpDNA SequenceORF Start: at 1ORF Stop: TAA at 1645AACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAAAGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTAAGCTGCGCCTGGACCGGAAACCCATCCCTGACCATCTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGGTTCGGAAATGAAGTCGGGAGCCGGGCTGGAAGCAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTGTCAGCCAAAAATGATATCCGAGTGGAAATTGTCCACAAGGAACCAGCCTCTGGTCGGGAGGGTGAGGAGCACTCCACCATCAAGCAGCTGATGATGGACCGGGGTGAATTCCAGCAAGACTCAGTCCTGAAACAGCTGGAAGGTCCTCAAAGAAGAGGAGAAGAGTTTCAGAACCTGAAGGACCCCACCAATGGCTACTACAGCGTCAACACCTTCAAAGAGCACCACTCAACCCCGACCATCTCCCTCTCCAGCTGCCAGCCCGACCTGCGTCCTGCGGGCAAGCAGCGTGTGCCCACAGGCATGTCCTTCACCAACATCTACAGCACCCTGAGCGGCCAGGGCCGCCTCTACGACTACGGGCAGCGGTTTGTGCTGGGCATGGGCAGCTCGTCCATCGAGCTTTGTGAGCGGGAGTTCCAGAGAGGCTCCCTCAGCGACAGCAGCTCCTTCCTGGACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAGGATGGCTATGTGCAGTTCGACAAGGCCAGCAAGGCTTCTGCTTCCTCCTCCCACCACTCCCAGTCCTCGTCCCAGAACTCTGACCCCAGTCGACCCCTGCAGCGGCGGATGCAGACTCACGTCTAAGGATCACACACCGCGGGTGGGGACGGGCCAGGGAAGAGGTCAGGGCACGTTCTGGTTGTCCAGGGACTGTGGGGTACTTTACAGAGGACACCAGAATGGCCCACTTCCAGGACAGCCTCCCAGCGCCTCTGCCACTGCCTTCCTTCGAAGCTCTGATCANOV30b, CG55784-01Protein SequenceSEQ ID NO: 754548 aaMW at 59933.8kDNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTKALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAILSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVPADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHVNOV30c, 312000579SEQ ID NO: 7551630 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCAACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAACGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTCAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTGTCAGCCAAAAATGATATCCGAGTGGAAATTGTCCACAAGGAACCAGCCTCTGGTCGGGAGGGTGAGGAGCACTCCACCATCAAGCAGCTGATGATGGACCGGGGTGAATTCCAGCAAGACTCAGTCCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAGAGTTTCAGAACCTGAAGGACCCCACCAATGGCTACTACAGCGTCAACACCTTCAAAAGAGCACCACTCAACCCCGACCATCTCCCTCTCCAGCTGCCAGCCCGACCTGCGTCCTGCGGGTAAGCAGCGTGTGCCCACAGGCATGTCCTTCACCAACATCTACAGCACCCTGAGCGGCCAGGGCCGCCTCTACGACTACGGGCAGCGGTTTGTGCTGGGCATGGGCAGCTCGTCCATCGAGCTTTGTGAGCGAAAGTTCCAGAGAGGCTCCCTCAGCGACAGCAGCTCCTTCCTGGACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAGGATGGCTATGTGCAGTTCGACAAGGCCAGCAAGGCTTCTGCTTCCTCCTCCCACCACTCCCAGTCCTCGTCCCAGAACTCTGACCCCAGTCGACCCCTGCAGCGGCGGATGCAGACTCACGTCCCCGGGGGCNOV30c, 312000579Protein SequenceSEQ ID NO: 756543 aaMW at 59393.1kDTGSTNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRNTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNTYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGNOV30d, 311333341SEQ ID NO: 7571036 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCAACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAACGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTCAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTGTCAGCCAAAAATGATATCCGAGTGGAACCCGGGGGCNOV30d, 311333341Protein SequenceSEQ ID NO: 758345 aaMW at 37329.1kDTGSTNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEPGGNOV30e, 311333338SEQ ID NO: 7591072 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCAACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAACGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTCAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGGTTCGGAAATGAAGTCGGGAGCCGGGCTGGAAGCAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTGTCAGCCAAAAATGATATCCGAGTGGAACCCGGGGGCNOV30e, 311333338Protein SequenceSEQ ID NO: 760357 aaMW at 38447.3kDTGSTNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEPGGNOV30f, 311346885SEQ ID NO: 761616 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCGAAATTGTCCACAAGGAACCAGCCTCTGGTCGGGAGGGTGAGGAGCACTCCACCATCAAGCAGCTGATGATGGACCGGGGTGAATTCCAGCAAGACTCAGTCCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAAGAGTTTCAGAACCTGAAGGACCCCACCAATGGCTACTACAGCGTCAACACCTTCAAAGAGCACCACTCAACCCCGACCATCTCCCTCTCCAGCTGCCAGCCCGACCTGCGTCCTGCGGGCAAGCAGCGTGTGCCCACAGGCATGTCCTTCACCAACATCTACAGCACCCTGAGCGGCCAGGGCCGCCTCTACGACTACGGGCAGCGGTTTGTGCTGGGCATGGGCAGCTCGTCCATCGAGCTTTGTGAGCGGGAGTTCCAGCAAGCAGGATGGCTATGTGCAGTTCGACAAGGCCAGCAAGGCTTCTGCTTCCTCCTCCCACCACTCCGGGCNOV30f, 311346885Protein SequenceSEQ ID NO: 762205 aaMW at 22667.6kDTGSEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNTYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHVPGNOV30g, 311333299SEQ ID NO: 7631666 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCAACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAACGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTCAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGGTTCGGAAATGAAGTCGGGAGCCGGGCTGGAAGCAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTGTCAGCCAAAAATGATATCCGAGTGGAAATTGTCCACAAGGAACCAGCCTCTGGTCGGGAGGGTGAGGAGCACTCCACCATCAAGCAGCTGATGATGGACCGGGGTGAATTCCAGCAAGACTCAGTCCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAAGAGTTTCAGAACCTGAAGGACCCCACCAATGGCTACTACAGCGTCAACACCTTCAAAGAGCACCACTCAACCCCGACCATCTCCCTCTCCAGCTGCCAGCCCGACCTGCGTCCTGCGGGCAAGCAGCGTGTGCCCACAGGCATGTCCTTCACCAACATCTACAGCACCCTGAGCGGCCAGGGCCGCCTCTACGACTACGGGCAGCGGTTTGTGCTGGGCATGGGCAGCTCGTCCATCGAGCTTTGTGAGCGGGAGTTCCAGAGAGGCTCCCTCAGCGACAGCAGCTCCTTCCTGGACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAGGATGGCTATGTGCAGTTCGACAAGGCCAGCAAGGCTTCTGCTTCCTCCTCCCACCACTCCCAGTCCTCGTCCCAGAACTCTGACCCCAGTCGACCCCTGCAGCGGCGGATGCAGACTCACGTCCCCGGGGGCNOV30g, 311333299Protein SequenceSEQ ID NO: 764555 aaMW at 60511.3kDTGSTNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCPAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHVPGGNOV30h, CG55784-02SEQ ID NO: 765891 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAACGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTCAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGGTTCGGAAATGAAGTCGGGAGCCGGGCTGNOV30h, CG55784-02Protein SequenceSEQ ID NO: 766297 aaMW at 32432.3kDNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCEAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLNOV30i,SNP13376439 ofSEQ ID NO: 7671806 bpCG55784-01,ORF Start: at 1ORF Stop: TAA at 1645DNA SequenceSNP Pos: 1305SNP Change: C to TAACAAAGCCATCCCCGGAGGAAAGGAGACGTCGGTCACCATTGACATCCAGCACCCTCCACTGGTCAACCTCTCGGTGGAGCCACAGCCAGTGCTGGAGGACAACGTCGTCACTTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAGCGGGGCCAGATCATCAAGGAGGCATCTGGAGAGGTGTACAGGACCACAGTGGACTACACGTACTTCTCAGAGCCCGTCTCCTGTGAGGTGACCAAAGCCCTGGGCAGCACCAACCTCAGCCGCACGGTTGACGTCTACTTTGGGCCCCGGATGACCACAGAACCCCAATCCTTGCTCGTGGATCTGGGCTCTGATGCCATCTTAAGCTGCGCCTGGACCGGCAACCCATCCCTGACCATCGTCTGGATGAAGCGGGGCTCCGGAGTGGTCCTGAGCAATGAGAAGACCCTGACCCTCAAATCCGTGCGCCAGGAGGACGCGGGCAAGTACGTGTGCCGGGCTGTGGTGCCCCGTGTGGGAGCCGGGGAGAGAGAGGTGACCCTGACCGTCAATGGACCCCCCATCATCTCCAGCACCCAGACCCAGCACGCCCTCCACGGCGAGAAGGGCCAGATCAAGTGCTTCATCCGGAGCACGCCGCCGCCGGACCGCATCGCCTGGTCCTGGAAGGAGAACGTTCTGGAGTCGGGCACATCGGGGCGCTATACGGTGGAGACCATCAGCACCGAGGAGGGCGTCATCTCCACCCTGACCATCAGCAACATCGTGCGGGCCGACTTCCAGACCATCTACAACTGCACGGCCTGGAACAGCTTCGGCTCCGACACTGAGATCATCCGGCTCAAGGAGCAAGGTTCGGAAATGAAGTCGGGAGCCGGGCTGGAAGCAGAGTCTGTGCCGATGGCCGTCATCATTGGGGTGGCCGTAGGAGCTGGTGTGGCCTTCCTCGTCCTTATGGCAACCATCGTGGCGTTCTGCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTGTCAGCCAAAAATGATATCCGAGTGGAAATTGTCCACAAGGAACCAGCCTCTGGTCGGGAGGGTGAGGAGCACTCCACCATCAAGCAGCTGATGATGGACCGGGGTGAATTCCAGCAAGACTCAGTCCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAAGAGTTTCAGAACCTGAAGGACCCCACCAATGGCTACTACAGCGTCAACACCTTCAAAGAGCACCACTCAACCCCGACCATCTCCCTCTCCAGCTGCCAGCCCGACCTGCGTCCTGCGGGTAAGCAGCGTGTGCCCACAGGCATGTCCTTCACCAACATCTACAGCACCCTGAGCGGCCAGGGCCGCCTCTACGACTACGGGCAGCGGTTTGTGCTGGGCATGGGCAGCTCGTCCATCGAGCTTTGTGAGCGGGAGTTCCAGAGAGGCTCCCTCAGCGACAGCAGCTCCTTCCTGGACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAGGATGGCTATGTGCAGTTCGACAAGGCCAGCAAGGCTTCTGCTTCCTCCTCCCACCACTCCCAGTCCTCGTCCCAGAACTCTGACCCCAGTCGACCCCTGCAGCGGCGGATGCAGACTCACGTCTAAGGATCACACACCGCGGGTGGGGACGGGCCAGGGAAGAGGTCAGGGCACGTTCTGGTTGTCCAGGGACTGTGGGGTACTTTACAGAGGACACCAGAATGGCCCACTTCCAGGACAGCCTCCCAGCGCCTCTGCCACTGCCTTCCTTCGAAGCTCTGATCANov30i,SNP1 3376439 ofCG55784-01,SEQ ID NO: 768MW at 59933.8kDProtein SequenceSNP Pos: 435548 aaSNP Change: Gly to GlyNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRTTVDYTYFSEPVSCEVTKALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAILSCAWTGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHV


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 30B.

TABLE 30BComparison of the NOV30 protein sequences.NOV30aMKPFQLDLLFVCFFLFSQELGLQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQVVVNOV30b------------------------------------------------------------NOV30c------------------------------------------------------------NOV30d------------------------------------------------------------NOV30e------------------------------------------------------------NOV30f------------------------------------------------------------NOV30g------------------------------------------------------------NOV30h------------------------------------------------------------NOV30aSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQNOV30b------------------------------------------------------------NOV30c------------------------------------------------------------NOV30d------------------------------------------------------------NOV30e------------------------------------------------------------NOV30f------------------------------------------------------------NOV30g------------------------------------------------------------NOV30h------------------------------------------------------------NOV30aDDAVYECQAIQAAIRSRPARLTVLVPPDDPVILGGPVISLRAGDPLNLTCHADNAKPAASNOV30b-------------------------------------------------------------NOV30c-------------------------------------------------------------NOV30d-------------------------------------------------------------NOV30e-------------------------------------------------------------NOV30f-------------------------------------------------------------NOV30g-------------------------------------------------------------NOV30h-------------------------------------------------------------NOV30aIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETNOV30b--------------------------------------------------NKAIPGGKETNOV30c----------------------------------------------TGSTNKAIPGGKETNOV30d----------------------------------------------TGSTNKAIPGGKETNOV30e----------------------------------------------TGSTNKAIPGGKETNOV30f-------------------------------------------------TGSEIVHKEPANOV30g----------------------------------------------TGSTNKAIPGGKETNOV30h--------------------------------------------------NKAIPGGKETNOV30aSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30bSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30cSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30dSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30eSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30fSGREGEEHSTIKQLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHNOV30gSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30hSVTIDIQHPPLVNLSVEPQPVLEDNVVTFHCSAKANPAVTQYRWAKRGQIIKEASGEVYRNOV30aTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNOV30bTTVDYTYFSEPVSCEVTKALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAILSCAWTGNOV30cTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNOV30dTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNOV30eTTVDYTYFSEPVSCEVTNALGSThLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNOV30fSTPTISLSSCQPDLRPAGKQRVPTGMSFTNIYSTLSGQGR----LYDYGQRFVLGMGSS-NOV30gTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNOV30hTTVDYTYFSEPVSCEVTNALGSTNLSRnTDVYFGPRMTTEPQSLLVDLGSDAIFSCAWTGNOV30aNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCBAVVPRVGAGEREVTLTVNGPPNOV30bNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPNOV30cNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPNOV30dNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPNOV30eNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPNOV30fSIELCEREFQRGS---LSDSSSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQ-NOV30gNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPNOV30hNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPNOV30aIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVINOV30bIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVINOV30cIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVINOV30dIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVINOV30eIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETISTEEGVINOV30f-S-SSQNSDPSRPLQRRMQTHVPGG-----------------------------------NOV30gIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETTSTEEGVINOV30hIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETTSTEEGVINOV30aSTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQ------------ESVPMAVIIGVNOV30bSTLTISNIVPADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVNOV30cSTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQ------------ESVPMAVIIGVNOV30dSTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQ------------ESVPMAVIIGVNOV30eSTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVNOV30f------------------------------------------------------------NOV30gSTLTISNIVPADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMAVIIGVNOV30hSTLTISNIVHADFQTIYNCTAWNSFGSDTEIIRLKEQG-------------------SEMNOV30aAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQNOV30bAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQNOV30cAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQNOV30dAVGAGVAFLVLMATIVAFCCARSQHNLKGVVSAKNDIRVEPGG-----------------NOV30eAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEPGG-----------------NOV30f------------------------------------------------------------NOV30gAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPASGREGEEHSTIKQNOV30hKSGAGL------------------------------------------------------NOV30aLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDNOV30bLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDNOV30cLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDNOV30d------------------------------------------------------------NOV30e------------------------------------------------------------NOV30f------------------------------------------------------------NOV30gLMMDRGEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDNOV30h------------------------------------------------------------NOV30aLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSNOV30bLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSNOV30cLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSNOV30d------------------------------------------------------------NOV30e------------------------------------------------------------NOV30f------------------------------------------------------------NOV30gLRPAGKQRVPTGMSFTNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSNOV30h------------------------------------------------------------NOV30aSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHV--NOV30bSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHV--NOV30cSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHVPGNOV30d------------------------------------------------------------NOV30e------------------------------------------------------------NOV30f------------------------------------------------------------NOV30gSFLDTQCDSSVSSSGKQDGYVQFDKASKASASSSHHSQSSSQNSDPSRPLQRRMQTHVPGNOV30h------------------------------------------------------------NOV30a-NOV30b-NOV30cGNOV30d-NOV30e-NOV30f-NOV30gGNOV30h-NOV30a(SEQ ID NO: 752)NOV30b(SEQ ID NO: 754)NOV30c(SEQ ID NO: 756)NOV30d(SEQ ID NO: 758)NOV30e(SEQ ID NO: 760)NOV30f(SEQ ID NO: 762)NOV30g(SEQ ID NO: 764)NOV30h(SEQ ID NO: 766)


Further analysis of the NOV30a protein yielded the following properties shown in Table 30C.

TABLE 30CProtein Sequence Properties NOV30aSignalP analysis:Cleavage site between residues 24 and 25PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 7; pos. chg 1; neg. chg 1H-region:length 11; peak value 13.09PSG score:8.69GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −3.29possible cleavage site: between 17 and 18>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 524-540−10.40PERIPHERALLikelihood = 1.54 (at 65)ALOM score: −10.40 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 531Charge difference: 5.0 C(3.0)-N(−2.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptide>>> membrane topology: type 1b (cytoplasmic tail 524 to 766)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):8.25Hyd Moment(95):6.93G content:0D/E content:2S/T content:1Score: −6.35Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: KRGCCLVLGYMAKDKFR at 24content of basic residues: 10.2%NLS Score: 0.02KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs:Leucine zipper pattern (PS00029): *** found *** LLFVCFFLFSQELGLQKRGCCL at 8nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):39.1%: nuclear21.7%: cytoplasmic17.4%: mitochondrial 8.7%: vesicles of secretory system 4.3%: vacuolar 4.3%: peroxisomal 4.3%: endoplasmic reticulum>> prediction for CG55784-03 is nuc (k = 23)


A search of the NOV30a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 30D.

TABLE 30DGeneseq Results for NOV30aNOV30aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG96273Human immunoglobulin superfamily1 . . . 766766/766(100%)0.0protein IGSFP-11 - Homo sapiens, 7661 . . . 766766/766(100%)aa. [WO200272794-A2, 19-SEP-2002]ABU58692Human PRO polypeptide #293 - Homo1 . . . 557554/569(97%)0.0sapiens, 600 aa. [US2003027272-A1,1 . . . 569555/569(97%)06-FEB-2003]ABU57223Human PRO polypeptide #293 - Homo1 . . . 557554/569(97%)0.0sapiens, 600 aa. [US2003027280-A1,1 . . . 569555/569(97%)06-FEB-2003]ABU56228Human secreted/transmembrane1 . . . 557554/569(97%)0.0protein, PRO19814 - Homo sapiens,1 . . . 569555/569(97%)600 aa. [US2003022298-A1, 30-JAN-2003]ABU10802Human secreted/transmembrane1 . . . 557554/569(97%)0.0protein #293 - Homo sapiens, 600 aa.1 . . . 569555/569(97%)[US2002127584-A1, 12-SEP-2002]


In a BLAST search of public sequence databases, the NOV30a protein was found to have homology to the proteins shown in the BLASTP data in Table 30E.

TABLE 30EPublic BLASTP Results for NOV30aNOV30aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8IZU9NPEH2 - Homo sapiens (Human),1 . . . 766766/778 (98%)0.0778 aa.1 . . . 778766/778 (98%)Q96JG0Hypothetical protein KIAA1867 -1 . . . 766766/778 (98%)0.0Homo sapiens (Human), 779 aa2 . . . 779766/778 (98%)(fragment).AAO41835Membrane protein mKirre - Mus1 . . . 766750/766 (97%)0.0musculus (Mouse), 766 aa.1 . . . 766762/766 (98%)Q8BR86Weakly similar to NEPH1 - Mus1 . . . 766750/778 (96%)0.0musculus (Mouse), 778 aa.1 . . . 778762/778 (97%)Q8BGQ5Weakly similar to NEPH1 - Mus1 . . . 591575/591 (97%)0.0musculus (Mouse), 612 aa.1 . . . 591588/591 (99%)


PFam analysis predicts that the NOV30a protein contains the domains shown in the Table 30F.

TABLE 30FDomain Analysis of NOV30aIdentities/SimilaritiesPfamNOV30afor theExpectDomainMatch RegionMatched RegionValueig 62 . . . 12914/72 (19%)0.0002241/72 (57%)ig163 . . . 22913/69 (19%)0.004543/69 (62%)ig349 . . . 40015/55 (27%)1.6e−0639/55 (71%)ig433 . . . 50112/70 (17%)0.3145/70 (64%)


Example 31

The NOV31 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 31A.

TABLE 31ANOV31 Sequence AnalysisNOV31a, CG55790-02SEQ ID NO: 7698250 bpDNA SequenceORF Start: ATG at 4ORF Stop: TAG at 1423ACCATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTGAGTTTGCCGTGGGAAGCAGCAGGTTCTGGGGGGCCCAGGGGAGGCTTGGCTGCCAGCTGTCTTTCAGAGTTTCAAAAAACTTTCAAAAGGCAAAAGTCCCTTGCCTTGAACAACTGTTGTTCCTGGAGACGCAGCGAAGCCCTCGATGGTGCGCATGGCATTTCCTGCAGCCTCCCCTTGGCATGGGATGGCATCCTGGTGTGCACTTTGTCACACTGCGATGGGATTTTCCCAACATGCACAGAAGCAGAGAGACGAGTGCTAGACCCCCGCGCTCCCCAGTGCCCAGCCCCGACCAGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCCATGGGGTGTCCGGAGTGGGTCCAGGCACCGGCGCCCAGCCCCCGTGGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCTGTGGGGTGTCCGGAGTGGGTCCGGGCACCGCCAGCTTCTCTCTGTGGCAGCCACTCCTGCAGCTCTCGTTTGCCCCTCAGTTCCAGGAGCAACATAGATGTGGATTCCTGTCCAATTTGGGAAAAATGTCCACACACGGTCACCCACCTGGCAGGTGCCTCTGGCTGCAAGGGGCGCTGGGCTTCGCAGGCAGGCCAGCCGGGCTCCCCGCCATGGGCCAGGATCCCCTCCGAGCGCTGTTTGCCGCCCAGGAGAAGGGGTTCCCCGGGGACAGTGGGCTCAGGGTGTGCGCAGCCACCATGCTGTGGTGTCACCTGTGGACCCAGGCGAGCTGATGGCCGACCGCAGAAACGCACTTCCAAGGCCAGGTCGGCCCATCCAGATGATGCAGGAACACAGCTTGCTAAAAACACGGCCGGCCTGTTCCCGTCGGAGCCAGTCGAAGTTCCCTGAACAGGCCGCTGTTTCCGAAGCTTTAAACCCTGTGTTTCCACCAAGCTGAGTCCTGAGAAAACCGACGTCTGCCTGCAGAAGGGAAAGGGGTGCTTCATGTTCCTCTCTCTCCTTCATCTCCCTTCCAAGGCCACGTTTGACCGGAGCTCACCGCCCAGAGCGTGGACAGGGCTTCCGTGAGACGCCACCGTGAGAGGCCAGGTGGCAGCTTGAGCATGGACTCCCAGACTGCAGGGGAGCACTTGGGGCAGCCCCCAGAAGGACCACTGCTGGATCCCAGGGAGAACCTGCTGGCGTTGGCTGTGATCCTGGAATGAGGCCCTTTCAAAAGCGTCATCCACACCAAAGGCAAATGTCCCCAAGTGAGTGGGCTCCCCGCTGTCACTGCCAGTCACCCACAGGAAGGGACTGGTGATGGGCTGTCTCTACCCGGAGCGTGCGGGATTCAGCACCAGGCTCTTCCCAGTACCCCAGACCCACTGTGGGTCTTCCCGTGGGATGCGGGATCCTGAGACCGAAGGGTGTTTGGTTTAAAAAGAAGACTGGGCGTCCGCTCTTCCAGGACGGCCTCTGTGCTGCTGGGGTCACGCGAGGCTGTTTGCAGGGGACACGGTCACAGGAGCTCTTCTGCCCTGAACGCTCCCAACCTGCCTCCCGCCCGGAAGCCACAGGACCCACTCATGTGTGTGCCCACAAGTGTAGTTAGCCGTCCACACCGAGGAGCCCCCGGAAGTCCCCACTGGGCTTCAGTGTCCTCTGCCACATTCCCTGGGAGGAACAATGTCCCTCGGCTGTTCCGGTGAAAAGTTGAGCCACCTTTGGAAGACGCACGGGTGGAGTTTGCCAGAAGAAAGGCTGTGCCAGGGCCGTGTTTGGCTACAGGGGCTGCCGGGGCTCTTGGCTCTGCAGCGAGAAAGACACAGCCCAGCAGGGCTGGAGACGCCCATGTCCAGCAGGCGCAGGCCTGGCAACACGGTCCCCAGAGTCCTGAGCAGCAGTTAGGTGCATGGAGAGGGTATCACCTGGTGGCCACAGTCCCCCTTCTCACCTCAGCAATGATCCCCAAAGTGAGAGCTGGCTCCCCCGGCCCCCACCACCCTCAGCAGCCCCACCCCACTCAACCCTGAGGGTCCCCAGGGTCCTGATGAAGACCTCCGACCCCAGCGCCAGGCTCCTCGGAGCCCAACAGTCCCAAGGGGGCAGGAGACGGGGTGGTCCAGTGCTGAGGGGTACAGCCCTGGGCCCTGACCAGCCCCGGCACCTGCCATGCTGGTTCCCCGAATGAATCAGCTGCTGACTGTCTCCAGAAGGGCTGGAAAGGATGCTGCCAGGTGACCCGAGGTGCACTCGCCCCAGGGAGATGGAGTAGACAGCCTGGCCTGGCCCTCGGGACACATTGTCTGCCCCGGGGCTATGGGCAAATGCCCCTCCTTCTTACTTCCCAGAATCCCCTGACATTCCCAGGGTCAGCCAGGACCTGTTAAAGCCCTGGTCACTTGGAACTGACAGCTGTGTGAGGCCTGCACTTCTCAGACCCAGACTTAGAACAAAAGGAGGAGTGAGGACTCAAGGCTACAATGAGGTTCCAGTACTTGTTACAAGAAATTGGTTTTCTGCAAAAGATGTGTGGTGTTTATAAAAGAACAGTTGTCATCACCGGGCATTGATTGGCAGGGACAAGGAGCTGCTTGGGTGTGGAAAGTTGGGGCGTTGGAAAGTGGGCTGTGGTGCCCATTTGCAGTGACTGTGAAGTGACTCCAGGACGGACCTGCGGGGGCACCCAGAGGTCCTAAGCCCCAGGACTGAGGGTCGTGCATCACCACTCGGGTGTCCCGGGAGGTGCCCTGGGCCCGGGGACCTCACAGGCAGGACGGCGACACTAATGCAGGGAGAGGGAGTCTGGCCCCAGCTTTTCCTATCAGAGGCGATTTTCCTTCACCAGGGGATGGGCAGGAAAGAGGCAGGGGCCCCAGAAGCTTCTGTCCCTCATGCCTGAGGGCACGGGGGACACTTGGAGGCTGCTGTCACCACTGTGCGTCCAAGGCCATGCTCTCTGCGGGTCAGTGCCTGAGTCTCGCCTCCCTGCTGGTCCCTGAAGCCCCCTCAGAAGCCCTGCCTGTCACGTCGGCATTTGTGAGACCTACCCTGTAACGCCTGCCCCTCTCAGCCCAACATCAGCTTCCTCTTTCTCCCTTGCTGTAGACAGGCTGGATTCCAGTGTTGGGACAGCCATCTCCAGAAACCTGACTTAAGAGAGTAAGATGCAAATCGTGCCTGTATCCAGTGGCTTTGGTGGGTCCAGGGAGTCTTGGGCACAGCCAGCTCAGCTGTCTGTGGTATGAGCAGGAACAGGTGCCACTCCTGCTCAGGGGACCCTGCCCTACACCAGGCTGTTCCGTCCCCCTGGAGGACATGGGGCCAGGTCTGGAGGCATTTTGGGTTGTCACAGCTGGGGGCTGTTCCTCGGCTTCAGCGGGTGGAAGCCTCAGATGCTGTTCAACATCTTCTGGACACGGGAGGCCCCGACAGAGAGAAGCGTCCACCCGCAAGTCCACAGTCTGAGGTCTCCCCTCAGAGACCCTGCCCTGCACACCCACCTCCAGCCAAAGGTCCTGCCTGCCCCAGGGCTCAGGGGAACCTTGCCGGTCTGTGGAACAGGAGAGGGGACTCTCGCCAGCTGCACCACCCTGCACGTAGTAGGTGTGCGGTAAACATCCACCAGGGAGGCTCCAGTCAAGGCTGGCAGATGGGGCGGTCCATCCCTAGGGCAGGTGACAGAAGGGAAAAGGCTGCCTGCTGGCCCCCGAGCCAGGTAGCACATGCTTGTGCCTCAGTTTCCCCTCCTGTAAAGTGAGGCGCTGGATCCAGGTTCTGTCTACTGGGCTCTGCAGCTTGGACGCTCCTAAGACCAAGCGACCCACCCTGGGGAGGGCAGCTATGGCTTTGGAATAGCTGTCCAGGCCCGGGTGCCTCCAAGACGGCCACCACACCCTGCCTGTGCTGCAGGGGTGCAGGGGTAAGGGGCAAGACTCCAGAGGCCTCCTCTCTGCATCTCCTTGTCTTCAGTGGCCGGAGGTGAGGCCTGAGCTCAGGGGAGGGGCTTCTGCCACGAACCCTATGGCGGGGCACAGCACACTTTTCCCAGGGAGGACCCCTGGGCCCCCTGCATTATCCCCAGCGGACTGTGGGGTCACCTTCCAAGAGCGACATTGAGAAGCTCCAGCTCTAGGAGTGTGCAGACTCTTAACCAGGCAGGCCCAGGCCCTGGGGCACACAAAGGCGGGGCCTGCTCTCCCCAGCTGCCCCTGCCAATGGGGGCTGGACTGTCCTACCCTCCTCCCTTCTACCTCCCCACTGTCTTCCCTCTCCACTGTCACCACTGCCTCCCTCTTCCACTGTCCTCCATGCACTGCCCTCCCTCCACCTTCCCCCACCCCCACCACTCCCCATGCTGTCCCCAGGCTCCCCCCGCTCTCCCCCCTCCCCACTGTCCCCCTCCCCATGCTGTACCCAGCTCACCCCCCTCTCCCCTCTCCCCACTGTCCCCCCTCCCACTCCCCATGCTGTCCCCAGCTCACCCTACATGGACTTGGCGATGTCCTTCCATGGCTCACCGGTCTGAATTTCCATGATGAGCCGGGCCTGCAGCTTTGCTCCCCTATCCCTGCCCAGGCTGCAGCTGTCCATGCAGGGAGCGAGCTCCAGCACCTGCGGAGTCCTTCCGTGCGGGCCTCTCCGTGCCACAGCAGCCAGGGACCTCAGGTGCCTGTGCATGACACCACCGCCCATCCTCATCCTGAGCCAGCCTCTCAGGATCAGGACTTGGTTTGGCGGCGTTAACCTTAGAGCCTGCAAGGGGCTTCCTCCTGGTGGGTCTGGCCGTAGCCTGGGGAGGCCACAGCTCCAGGCCACTCCAGACCTCCCTTCCTCTGGGCCTTCCATGTGGTGGCAACCACCGCAGCTGTAAGGGAGGGAAAATGGAGCGTTTGTTCTCGGGCTGGGCTGGGGTCTGGGGGAAGCCATGGGCGTGAAGACTGGAGTATTATTTGATGGAGAAGCGGCCACTCCTGGAGACCGGCGGCAAACACAGAAGCACAGCGTGGAAGGTGCTGGTGTCAGCCCACACGGGTGATGGAGCCCCAGACACAATGCATTCCTGTTGCCCTCGCCCTCAGCCAGGCCCCACGCAGGCCAGGGAGCACTGGCAAAGCTTGGCAACCCTCTGGGGGCCAGCCTTCATCCAGGCCGAAGGTGGTCAGTGGCCCACCATGGCCCAGGTAGAAAACTCACGGATTAAGATTTCATGCCCGACTCCAAAGGCAAGAGACTTTATTATTTTATTTTTTTTGAGCCAGAGTATCGCTCTGTCACCTAGGCTGGAGTGCAATCTCTGCTCATTGCAACATCTGCCTCCCGAACTCAAGCAATTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGTGCGCCACCATGCCCAGGTAATTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGTCAGGCTGGTTTCAAACTCCTGACCTCAAATGATCTGCCCACCTCGACCTCCCAAAGTGCTGGGATTACAGGTGCGAGCCACCGCACCTGGCTACCAGACACTTCAGAGTTACAGGTTAGTTTTTCTTTTTCTTTTATTTTTTTTTTTTTGGCGGAGGTGCAGGGGGAGTTAAACAAACAAACAAAATAAACAGGCCGGGTGCGGTGGCTCATGCCTGTAATCCCAGCACTTTAGGAGGCCTAGGTGGGTGGATCACGAGATCAGGGGTTCAAGACCAGCCTGGCCGAGATGGTAAAACCCCGTCTCCACTAAAAATACAAAAATTGGCCAGGCACGGTGGCTCACACCTGTAATCCCAGTACTTTGGGAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGAGTTCAAGACCAACCTGACCAACATGGAGAAACCCCATCTCTACTAAAAATACAkAATTAGCCAGGTGTGGTGGTGCATGCCTGTAATTCCAGCTGCCATTGCACTCCAGCCTGGGAACAAGAGCGAAACTCTGACTAAAAAAGAAAGAAAGAAAGAAAAAAATTAGTTGGGCACGGTGGCAGGCGCCTGTAATCCCAGGTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAACCCGGGAGGCAGAGGTCGCAGTGAGCCGAGATTGCACCACTGCCCTCCAGCCTGGGTGACAGAGCAAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAATTGGATACATTGTAATACCTCAAATACTTGTAAGTGAAGCACCCCAGTTCCCATAGAGCTGCCGCACTCAGAGGCTTCTGTAACCTGCCTGCTCCCAGCATTCTATTTAGGGTCTGGTATGTCCAGAATTTGCAGACACAGCAATTCCTGCAGCAGCAGTGCACCATGTGCAAGGGGCCCCATGACCAGCCCACTGTGAGCTCACACGTGATGACTGAGGCTTCTTCACACAGCAGGGCTCTGGGTGTGATACCCAGGGCACACGCGTTTGCACAGGCACAGGCCACACAAGTTCTCACATGCTCAGCCCCATAAGCCGTGCTGGACAGGCATGGCCATTTACACCCAGGATCCTGCTGAGAACAGCAACCAACTCACCACCCTCGCATCATGATCCTTGCCACACAGGGGCTCTGGTGGCTTTGGTGGCCTGGGCTGTGGCTCTGCTGCCAGCCACCTTGAGTGAAGATCCGGGTTCTCTGGGTGCTACTCAGCTGCTATGTGGGGAGCTCTGTCTGCATACAGACTTCTCAGGGGAGTTCTCAGCTTGGACCCTTATCTCCCCAGAATCCTGGAACCTGCTCCTTCTGCTCTCGTGACTGACTGTGTTCTCTATGCAACTTCCAATAAAACCTCTTCATTTGAAAGGAAAAAAGTCTGCATTATCTGTTTAGGAAGGGAGAGAGTTCATATTGCAATCTTTTTTTTTTTAATTAAAATAATCTCAGCCTGGGCAACATGGTGAGACCCCATCTCTGTAAAACATTTTTAAAAAATTAGCCGGGTATGGTGGCGCACACTTGTAGTCCCAGCTACTCAGGAGGCTGAAGCGGGAGGATCCATTGAACCTGAGAAGTCGAAGCTGCAGTGAGCTGTGATTGTGCCACTGTACTCCAGCCTGGACAACAGAGTGAGACGCCGTCTCAAATAAATAAATACATNOV31a, CG55790-02Protein SequenceSEQ ID NO: 7701473 aaMW at 51924.6kDMRLGSPGLLFLLFSSLEADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVTLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNNRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVATGWVCRDRCLQHSYAGAWAVSPETELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLQPPLGMGWHPGVHFVTLRWDFPNMHRSRETSARPPRSPVPSPDQGVQGGSRHRRPAPMGCPEWVQAPAPSPRGVSPAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGATNOV31b, 258668431SEQ ID NO: 7711393 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCATTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTGAGTTTGCCGTGGGAAGCAGCAGGTTCTGGGGGGCCCAGGGGAGGCTTGGCTGCCAGCTGTCTTTCAGAGTTTCAAAAAACTTTCAAAAGGCAAAAGTCCCTTGCCTTGAACAACTGTTGTTCCTGGAGACGCAGCGAAGCCCTCGATGGTGCGCATGGCATTTCCTGCAGCCTCCCCTTGGCATGGGATGGCATCCTGGTGTGCACTTTGTCACACTGCGATGGGATTTTCCCAACATGCACAGAAGCAGAGAGACGAGTGCTAGACCCCCGCGCTCCCCAGTGCCCAGCCCCAACCAGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCCATGGGGTGTCCGGAGTGGGTCCAGGCACCGGCGCCCAGCCCCCGTGGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCTGTGGGGTGTCCGGAGCGGGTCCGGGCACCGCCAGCTTCTCTCTGTGGCAGCCACTCCTGCAGCTCTCGTTTGCCCCTCAGTTCCAGGAGCAACACTCGAGGGCNOV31b, 258668431Protein SequenceSEQ ID NO: 772464 aaMW at 50961.4kDTRSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSIEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNNRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEIGAATWSILAVLCLLVVVAVAIGIWCRDRCLQHSYAGAWAVSPETELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLQPPLGMGWHPGVHFVTLRWDFPNMHRSRETSARPPRSPVPSPNQGVQGGSRHRRPAPMGCPEWVQAPAPSPRGVSRAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGATLEGNOV31c, 309303509SEQ ID NO: 7731393 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCATTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTGAGTTTGCCGTGGGAAGCAGCAGGTTCTGGGGGGCCCAGGGGAGGCTTGGCTGCCAGCTGTCTTTCAGAGTTTCAAAAAACTTTCAAAAGGCAAAAGTCCCTTGCCTTGAACAACTGTTGTTCCTGGAGACGCAGCGAAGCCCTCGATGGTGCGCATGGCATTTCCTGCAGCCTCCCCTTGGCATGGGATGGCATCCTGGTGTGCACTTTGTCACACTGCGATGGGATTTTCCCAACATGCACAGAAGCAGAGAGACGAGTGCTAGACCCCCGCGCTCCCCAGTGCCCAGCCCCGACCAGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCCATGGGGTGTCCGGAGTGGGTCCAGGCACCGGCGCCCAGCCCCCGTGGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCTGTGGGGTGTCCGGAGCGGGTCCGGGCACCGCCAGCTTCTCTCTGTGGCAGCCACTCCTGCAGCTCTCGTTTGCCCCTCAGTTCCAGGAGCAACACTCGAGGGCNOV31c, 309303509Protein SequenceSEQ ID NO: 774464 aaMW at 50962.4kDTRSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSIEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKAAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLQPPLGMGWHPGVHFVTLRWDFPNMHRSRETSARPPRSPVPSPDQGVQGGSRHRRPAPMGCPEWVQAPAPSPRGVSRAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGATLEGNOV31d, 315925314SEQ ID NO: 775871 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCGGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGCTCGAGGGCNOV31d, 315925314Protein SequenceSEQ ID NO: 776290 aaMW at 31946.7kDTGSDTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGHLLEGNOV31e, 315970230SEQ ID NO: 777928 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCGGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACCGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGCTCGAGGGCNOV31e, 315970230Protein SequenceSEQ ID NO: 778309 aaMW at 33978.1kDTGSTMRLGSPGLLFLLFSSLEADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYRINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGHLLEGNOV31f, CG55790-01SEQ ID NO: 779912 bpDNA SequenceORF Start: ATG at 4ORF Stop: TAA at 910ACCATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGTAANOV31f, CG55790-01Protein SequenceSEQ ID NO: 780302 aaMW at 33362.5kDMRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNNRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCPDRCLQHSYAGAWAVSPETELTGHLNOV31g, 0G55790-03SEQ ID NO: 781910 bpDNA SequenceORF Start: ATG at 2ORF Stop: TAA at 908CATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCATATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGAGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGTAANOV31g, CG55790-03Protein SequenceSEQ ID NO: 782302 aaMW at 33362.5kDMRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNEALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPWSAPHSPSQDELTFTCTSINGYPRPNVYVVINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGHLNOV31h, CG55790-04SEQ ID NO: 783705 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACNOV31h, CG55790-04Protein SequenceSEQ ID NO: 784235 aaMW at 26169.1kDDTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNNOV31i, CG55790-05SEQ ID NO: 785393 bpDNA SequenceORF Start: at 11ORF Stop: at 1385CACCAGATCTCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCATTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTGAGTTTGCCGTGGGAAGCAGCAGGTTCTGGGGGGCCCAGGGGAGGCTTGGCTGCCAGCTGTCTTTCAGAGTTTCAAAAAACTTTCAAAAGGCAAAAGTCCCTTGCCTTGAACAACTGTTGTTCCTGGAGACGCAGCGAAGCCCTCGATGGTGCGCATGGCATTTCCTGCAGCCTCCCCTTGGCATGGGATGGCATCCTGGTGTGCACTTTGTCACACTGCGATGGGATTTTCCCAACATGCACAGAAGCAGAGAGACGAGTGCTAGACCCCCGCGCTCCCCAGTGCCCAGCCCCAACCAGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCCATGGGGTGTCCGGAGTGGGTCCAGGCACCGGCGCCCAGCCCCCGTGGGGTGTCCAGGGCGGGTCCAGGCACCGGCGCCCAGCCCCTGTGGGGTGTCCGGAGCGGGTCCGGGCACCGCCAGCTTCTCTCTGTGGCAGCCACTCCTGCAGCTCTCGTTTGCCCCTCAGTTCCAGGAGCAACACTCGAGGGCNOV31i, CG55790-05Protein SequenceSEQ ID NO: 786458 aaMW at 50317.7kDLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSIEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSITAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLQPPLGMGWHPGVHFVTLRWDFPNNHRSRETSARPPRSPVPSPNQGVQGGSRHRRPAPMGCPEWVQAPAPSPRGVSRAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGATNOV31j, CG55790-06SEQ ID NO: 787928 bpDNA SequenceORF Start: ATG at 14ORF Stop: at 920CACCGGATCCACCATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCATTGAGGTTACACTCCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACCACGTGCTGAGGAATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGCTCGAGGGCNOV31j, CG55790-06Protein SequenceSEQ ID NO: 7881302 aaMW at 33376.5kDMRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSIEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGHLNOV31k, CG55790-07SEQ ID NO: 789871 bpDNA SequenceORF Start: at 11ORF Stop: at 863CACCGGATCCGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCTGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCGGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGTACATCCATAAACGGCTACCCCAGGCCCAACGTGTACTGGATCAATAAGACGGACAACAGCCTGCTGGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGCTCGAGGGCNOV31k, CG55790-07Protein SequenceSEQ ID NO: 790284 aaMW at 31402.1kDDTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNPALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLAVSPETELTGHLNOV31l,SNP1 3374852 ofSEQ ID NO: 791912 bpCG55790-01,ORF Start: ATG at 4ORF Stop: TAA at 910DNA SequenceSNP Pos: 296SNP Change: T to CACCATGCGGCTGGGCAGTCCTGGACTGCTCTTCCTGCTCTTCAGCAGCCTTCGAGCTGATACTCAGGAGAAGGAAGTCAGAGCGATGGTAGGCAGCGACGTGGAGCTCAGCTGCGCTTGCCCTGAAGGAAGCCGTTTTGATTTAAATGATGTTTACGTATATTGGCAAACCAGTGAGTCGAAAACCGTGGTGACCTACCACATCCCACAGAACAGCTCCTTGGAAAACGTGGACAGCCGCTACCGGAACCGAGCCCTGATGTCACCGGCCGGCATGCTGCGGGGCGACTTCTCCCAGCGCTTGTTCAACGTCACCCCCCAGGACGAGCAGAAGTTTCACTGCCTGGTGTTGAGCCAATCCCTGGGATTCCAGGAGGTTTTGAGCGTTGAGGTTACACTGCATGTGGCAGCAAACTTCAGCGTGCCCGTCGTCAGCGCCCCCCACAGCCCCTCCCAGGATGAGCTCACCTTCACGTGACCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATGACGTGGTCAGCGTGCTGAGGATCGCACGGACCCCCAGCGTGAACATTGGCTGCTGCATAGAGAACGTGCTTCTGCAGCAGAACCTGACTGTCGGCAGCCAGACAGGAAATGACATCGGAGAGAGAGACAAGATCACAGAGAATCCAGTCAGTACCGGCGAGAAAAACGCGGCCACGTGGAGCATCCTGGCTGTCCTGTGCCTGCTTGTGGTCGTGGCGGTGGCCATAGGCTGGGTGTGCAGGGACCGATGCCTCCAACACAGCTATGCAGGTGCCTGGGCTGTGAGTCCGGAGACAGAGCTCACTGGTCACCTGTAANOV31l,SNP13374852 ofCG55790-01,SEQ ID NO: 792MW at 33346.5kDProtein SequenceSNP Pos: 98302 aaSNP Change: Leu to ProMRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSPRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNDRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETELTGHL


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 31B.

TABLE 31BComparison of the NOV31 protein sequences.NOV31a----MRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31b----------------TRSLPADTQEKEVRANVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31c----------------TRSLRADTQEKEVPAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31d-------------------TGSDTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31eTGSTMRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31f----MRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31g----MRLGSPGLLFLLFSSLRADTQEKEVRANVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31h----------------------DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31i-------------------LRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV31j----MRLGSPGLLFLLFSSLRADTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTNOV3lk----------------------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-------------------------------------------NOV31iGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETNOV31jGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETNOV31kGERDKITENPVSTGEKNAATWSILAVLCLLVVVAVAIGWVCRDRCLQHSYAGAWAVSPETNOV31aELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLNOV31bELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLNOV31cELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLNOV31dELTGHLLEG---------------------------------------------------NOV31eELTGHLLEG---------------------------------------------------NOV31fELTGHL------------------------------------------------------NOV31gELTGHL------------------------------------------------------NOV31h------------------------------------------------------------NOV31iELTGEFAVGSSRFWGAQGRLGCQLSFRVSKNFQKAKVPCLEQLLFLETQRSPRWCAWHFLNOV31jELTGHL------------------------------------------------------NOV31kELTGHL------------------------------------------------------NOV31aQPPLGMGWHPGVHFVTLRWDFPNMHRSRETSARPPRSPVPSPDQGVQGGSRHRRPAPMGCNOV31bQPPLGMGWHPGVHFVTLRWDFPNMHRSRETSARPPRSPVPSPNQGVQGGSRHRRPAPMGCNOV31cQPPLGMGWHPGVHFVTLRWDFPNMHRSRETSARPPRSPVPSPDQGVQGGSRHRRPAPMGCNOV31d------------------------------------------------------------NOV31e------------------------------------------------------------NOV31f------------------------------------------------------------NOV31g------------------------------------------------------------NOV31h------------------------------------------------------------NOV31iQPPLGMGWHPGVHFVTLRWDFPNNHRSRETSARPPRSPVPSPNQGVQGGSRHRRPAPMGCNOV31j------------------------------------------------------------NOV31k------------------------------------------------------------NOV31aPEWVQAPAPSPRGVSEAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGAT---NOV31bPEWVQAPAPSPRGVSRAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGATLEGNOV31cPEWVQAPAPSPRGVSRAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGATLEGNOV31d------------------------------------------------------------NOV31e------------------------------------------------------------NOV3lf------------------------------------------------------------NOV31g------------------------------------------------------------NOV31h------------------------------------------------------------NOV31iPEWVQAPAPSPRGVSPAGPGTGAQPLWGVRSGSGHRQLLSVAATPAALVCPSVPGAT---NOV31j------------------------------------------------------------NOV31k------------------------------------------------------------NOV31a(SEQ ID NO: 770)NOV31b(SEQ ID NO: 772)NOV31c(SEQ ID NO: 774)NOV31d(SEQ ID NO: 776)NOV31e(SEQ ID NO: 778)NOV3lf(SEQ ID NO: 780)NOV3lg(SEQ ID NO: 782)NOV3lh(SEQ ID NO: 784)NOV31i(SEQ ID NO: 786)NOV31j(SEQ ID NO: 788)NOV31k(SEQ ID NO: 790)


Further analysis of the NOV31 a protein yielded the following properties shown in Table 31C.

TABLE 31CProtein Sequence Properties NOV31aSignalP analysis:Cleavage site between residues 19 and 20PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 2; pos. chg 1; neg. chg 0H-region:length 14; peak value 10.56PSG score:6.16GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 1.98possible cleavage site: between 20 and 21>>> Seems to have a cleavable signal peptide (1 to 20)ALOM:Klein et al's method for TM region allocationInit position for calculation: 21Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 258-274−13.27PERIPHERALLikelihood = 2.97 (at 127)ALOM score: −13.27 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 10Charge difference: −2.0 C(0.0)-N(2.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 275 to 473)MITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):5.99Hyd Moment(95):8.79G content:2D/E content:1S/T content:3Score: −2.92Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 27 LRA|DTNUCDISC: discrimination of nuclear localization signalspat4: RHRR (3) at 407pat7: nonebipartite: nonecontent of basic residues: 8.9%NLS Score: −0.29KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: RLGSnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 339LL at 454checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):55.6%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: extracellular, including cell wall>> prediction for CG55790-02 is end (k = 9)


A search of the NOV31a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 31D.

TABLE 31DGeneseq Results for NOV31aNOV31aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG30731Human B7-H2 transcript 2 (B7-H2 V2)1 . . . 473471/473(99%)0.0polypeptide - Homo sapiens, 473 aa.1 . . . 473473/473(99%)[WO200253733-A2, 11-JUL-2002]AAU00422Human B7-3 protein - Homo sapiens,1 . . . 473471/516(91%)0.0516 aa. [WO200112658-A2, 22-FEB-1 . . . 516471/516(91%)2001]AAU99789Human B7 related protein-1 (B7RP1)1 . . . 300300/300(100%)e−174#2 - Homo sapiens, 302 aa.1 . . . 300300/300(100%)[WO200244364-A2, 06-JUN-2002]AAG67292Amino acid sequence of a human hB7-1 . . . 300300/300(100%)e−174H2 polypeptide - Homo sapiens, 3021 . . . 300300/300(100%)aa. [WO200164704-A1, 07-SEP-2001]AAB08729Amino acid sequence of a human1 . . . 300300/300(100%)e−174B7RP1 polypeptide - Homo sapiens,1 . . . 300300/300(100%)302 aa. [WO200046240-A2, 10-AUG-2000]


In a BLAST search of public sequence databases, the NOV31 a protein was found to have homology to the proteins shown in the BLASTP data in Table 31E.

TABLE 31EPublic BLASTP Results for NOV31aNOV31aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueO75144ICOS ligand precursor (B7 homolog 2)1 . . . 300300/300(100%) e−174(B7-H2) (B7-like protein GI50) (B7-1 . . . 300300/300(100%)related protein-1) (B7RP-1) - Homosapiens (Human), 302 aa.CAD57064Sequence 11 from Patent WO0244364 -1 . . . 288288/288(100%) e−166Mus musculus (Mouse), 288 aa1 . . . 288288/288(100%)(fragment).Q9JHJ8ICOS ligand precursor (B7 homolog 2)7 . . . 295135/291(46%)2e−58(B7-H2) (B7-like protein GI50) (B7-32 . . . 317 172/291(58%)related protein-1) (B7RP-1) (LICOS) -Mus musculus (Mouse), 322 aa.O42404CD80-like protein - Gallus gallus2 . . . 28399/291(34%)6e−33(Chicken), 296 aa.3 . . . 288153/291(52%)Q9BXR1Costimulatory molecule - Homo2 . . . 27789/292(30%)3e−21sapiens (Human), 316 aa.4 . . . 270131/292(44%)


PFam analysis predicts that the NOV31a protein contains the domains shown in the Table 31F.

TABLE 31FDomain Analysis of NOV31aIdentities/SimilaritiesPfamNOV31afor theExpectDomainMatch RegionMatched RegionValueig30 . . . 11516/87 (18%)0.01160/87 (69%)


Example 32

The NOV32 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 32A.

TABLE 32ANOV32 Sequence AnalysisNOV32a, CG55906-04SEQ ID NO: 7934119 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 4117ATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGCGAGAGCCCGGCCGGCCGCTGACCCCACAGGAGCGCCTGCTGCCGAGGCTGCCCAACCACAGGCTCAGGTGGCTGCCCACCCAGAGCAGACGGCCCCATGGACGGAGAAGGAGCTGCAACCTTCGGAAAAGCAGATGGTGTCCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTAAGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCACCAAGACTGTCCTAACTGGCACCAAGAACACTGTCTGCAGTGGGGTGACCGGTGCCGTGAACTTGGCCAAAGAGGCCATCCAGGGGGGCCTGGATACCACCAAGTCTATGGTCATGGGTACGAAAGACACGATGTCCACTGGGCTCACAGGGGCAGCGAATGTGGCCAAGGGGGCCATGCAAACTGGGCTGAACACAACCCAAAATATCGCAACAGGTACAAAGGACACCGTCTGCAGTGGGGTGACTGGTGCCATGAATTTGGCCAGAGGAACCATCCAGACAGGCGTGGACACCACCAAGATCGTTCTAACTGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGCGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACGTGGCCAAAGGGACCGTCCAGACCGGCGTAGACACCACCAAGACTGTCCTAACCGGCACCAAGGACACCGTCTGCAGTGGGGTGACCAGTGCTGTGAACGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGTCTGTGGTCATAGGTACAAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCTGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTGACTACTGGGCTCGTGGGGGCAGTGAATGTCCTACAGTGGGGTCACCAGTGCCGTGAACGTGGCCAAGGGGGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCGACAGGTACAAAGAACACCTTTGGCAGTGGGGTGACCAGTGCTGTGAATGTGGCCAAAGGGGCTGCCCAGACAGGTGTAGACACGGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACTGTCCAGACCAGTGTGGACACCACCAAGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAAGGGGCCATCCAAGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAAGTTGGCCAAAGGGACTGTCCAGACCGGCATGGACACCACCAAGACTGTGTTAACTGGTACCAAGGATGCTGTGTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGATGGGTGTAGACACGGCCAAGACCGTGCTGACCGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAACGTGGCCAAGGGTGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCAACAGGTACAAAGAACACCCTTGGCAGTGGGGTGACCGGTGCTGCGAAAGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCCGTGTCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCGGTACCAAGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGGACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGACTACTGGAGTCACGGGGGCAGTGAATGTGGCCAAAGGAACCGTGCAGACCGGCGTGGACGCCTCCAAGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGACAGTGCTGACCGGAACCAAAGACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGACAATGGTGGGGAGCAGACTGCCCTGAGCCCCCAAGAGGCCAGCCACTACCAAGGGCCTTGCGACTGACGTGGCGACGTTCACCCAAGGGGCCGCCCCAGGCAGGGAGGACACGGGGCTTTTGGCCACCACACACGGCCCCGAAGAAGCCCCACGCTTGGCAATGCTGCAGAATGAGTTGGAGGGGCTGGGGGACATCTTCCACCCCATGAATGCGGAGGAGCAAGCTCAGCTGGCTGCCTCCCAGCCTGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTACTTCGTTCGTTTAGGTGACCTGGGTCCCAGCTTCCGCCAGCGGGCATTTGAACACGCGGTGAGCCACCTGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGCCCAGCTCCAGGACTGCTTCAGGCTGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGGAGCGGGATGCCGGGGTTCTGTCCAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGNOV32a, CG55906-04Protein SequenceSEQ ID NO: 7941372 aaMW at 136019.4kDMSAPDEGRRDPPKPKGKTLGSFFGSLPGFSSARNLVANAHSSARARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKQMVSGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVVQGGLDTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGAMNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVNVAKGAAQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTTLTGTKDTVCSGVTGAANVAKGAIQGGLDTTKSVLTGTKDAVSTGLTGAVKLAXGTVQTGMDTTKTVLTGTKDAVCSGVTGAANVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAANVAKGAVQTGLKTTQNIATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTALSPQEAPFSGISTPPDVLSVGPEPAWEAAATTKGLATDVATFTQGAAPGREDTGLLATTHGPEEAPRLAMLQNELEGLGDIFHPMNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGPSFRQPAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32b, CG55906-05SEQ ID NO: 7956600 bpDNA SequenceORF Start: ATG at 23ORF Stop: TAG at 4337TCCAGCTTCTCACGTTCTCACTATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAACTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGCGAGAGCCCGGCCGGCCGCTGACCCCACAGGAGCGCCTGCTGCCGAGGCTGCCCAACCACAGGCTCAGGTGGCTGCCCACCCAGAGCAGACGGCCCCATGGACGGAGAAGGAGCTGCAACCTTCGGAAAAGCAGATGGTGTCCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTAAGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCAAGGAGGCGGTGTCCAGCGGGGTCACAGGGGCCATGGACATGGCTAAGGGGGCCGTCCAAGGGGGTCTGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACCAGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCACCAAGACTGTCCTAACTGGCACCAAGAACACTGTCTGCAGTGGGGTGACCGGTGCCGTGAACTTGGCCAAAGAGGCCATCCAGGGGGGCCTGGATACCACCAAGTCTATGGTCATGGGTACGAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCCATGCAAACTGGGCTGAACACAACCCAAAATATCGCAACAGGTACAAAGGACACCGTCTGCAGTGGGGTGACTGGTGCCATGAATTTGGCCAGAGGAACCATCCAGACAGGCGTGGACACCACCAAGATCGTTCTAACTGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACGTGGCCAAAGGGACCGTCCAGACCGGCGTAGACACCACCAAGACTGTCCTAACCGGCACCAAGGACACCGTCTGCAGTGGGGTGACCAGTGCTGTGAACGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGTCTGTGGTCATAGGTACAAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCTGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTGACTACTGGGCTCGTGGGGGCAGTGAATGTCGCCAAAGGGACCGTCCAGACAGGCATGGACACCACCAAAACTGTCCTAACCGGTACCAAGGACACCATCTACAGTGGGGTCACCAGTGCCGTGAACGTGGCCAAGGGGGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCGACAGGTACAAAGAACACCTTTGGCAGTGGGGTGACCGGTGCTGTGAATGTGGCCAAAGGGGCCGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACTGTCCAGACCAGTGTGGACACCACCAAGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGACGGGTGTAGACACGGCCAAGACCGTGCTGACCGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGTGAACGTGGCCAAGGGTGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCAACAGGTACAAAGAACACCCTTGGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCATGGACACCACCAAGACTGTGTTAACTGGTACCAAGGATGCTGTGTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGACGGGTGTAGACACGGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACCGTCCAGACCAGTGTGGACACCACCAAGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGACACCGTATCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCGGTACCAAGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGCACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGACTACTGGAGTCACGGGGGCAGTGAATGTGGCCAAAGGAACCGTGCAGACCGGCGTGGACGCCTCCAAGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGACAGTGCTGACCGGAACCAAW3ACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGACAATGGTGGGGAGCAGACTGCCCTGAGCCCCCAAGAGGCCCCGTTCTCTGGCATCTCCACGCCCCCGGATGTGCTCAGTGTAGGCCCGGAGCCTGCCTGGGAAGCCGCAGCCACTACCAAGGGCCTTGCGACTGACGTGGCGACGTTCACCCAAGGGGCCGCCCCAGGCAGGGAGGACACGGGGCTTTTGACCACCACACACGGCCCCGAAGAAGCCCCACGCTTGGCAATGCTGCAGAATGAGTTGGAGGGGCTGGGGGACATCTTCCACCCCATGAATGCGGAGGAGCAAGCTCAGCTGGCTGCCTCCCAGCCCGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTACTTCGTTCGTTTAGGTGACCTGGGTCCCAGCTTCCGCCAGCGGGCATTTGAACACGCGGTGAGCCACCTGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGCCCAGCTCCAGGACTGCTTCAGGCTGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGGAGCGGGATGCCGGGGTTCTGTCCAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGCTGTAGGAGCCTGCAGGCCCGGCGCGGGGTCGCCCTGCTCTGTCCAGGGAGGAGCTGCCTCAGAACTTTCTCCCCGCCCCCAAACCTGGATCGGTTCCCTAAAGCCCTAGACCTTTGGGGCTGCAGCTGGCTGAGCGCCGAGGGGCTGCGGAGGCAGTGACCTTCTTAACTGAGCCACCCCACGCCCTGCTCCGGGCCTGCCTGCATCTCCCACCTCCTCCCCAGCGCTGCCTGCCCCTCTCGGAGCCTGGGGTCACTCAGACCACCAGCCAAGAGCCTTCCCTTGAAGTCCCCAAGCAAGCACTGCAATTAGGAAAGAGAAAAAGCAGCGTGCCCAGCCTGGAAGGGGATCTGTTTGCCCCGCTAGCAACCCTTTTATATCTAGCAGGGCTCTTCCAGTCCTGCAGCACGGGCCCCCAGCTATCAGCGGTGCAGGCAGTGCTGTGGCATCCCAGGCTCCGGGCAGCTCCGTTCTCATGCTGAAAGTGGGTCTCCGGCCTTAGCACACACACCTTGAGGGTCTTAAGAACCACATTCCCTCATAGTAGAAAGTACTAGAAAAAGCGACACTGCCATCATCATCCCAAGGCAGGCTGCTACTGCCTTTGCTGACCCCCGGGGTGGCCTCACGGTGGGGACAAAGCTGCCAGGAGCCACAGCAGCCACAGCTGGGGCTTTGCACCAGCCTGGCTTGAGACTGAGCAGTTTGCAGGGGGTGGGGGGTGCAAAAAACAAGCAAACAGGCTGCTGCTGCCTCCAGCTGCCCACCACAGGCCTGCCCCAGGCACCTGGGGCTCTGAGGCCCCTGGGGAGGCTGGGCCCAAAAGCTGCCCCTGGAGACACAGACAAAGGACTTCCCCGCAGGGAACTGTGCCCTATGGAGGGATCAGACAGGAACTGGGAACAGCCACAGAGGCTGCGTGCCTATGGCACAGCCCTTCCTCCGCCGCACACTCCCCCTGAATCCTCAGGCCCACCCAAGCGCCGGGCTGCAGAGGAAGCGGGGCTGGGGAGGCTGCAGGCATCAGAGACACTGGTGGTGGCGGACCCGGCCGCCGGGCCCCGTGCTCTCAGGCTAGCCCAGGTCGTGGAGGCTGGCAGGCTCAGGTCGGGTGTGAGACGTGCCGTGGCTGCGCTCAGTCCAGCGGGGAGGAGCCGTTCAGCCCAACCTCCCCAGGAAGCCATATCCCCACTCACCCGGTAAGAGAACCTTGTCGTCCCCTTTCCATGCTCTCCTAGGACACGAGCCCAGGAACCCCAGACCCAGGGGGAGGAAGGGTGGAGGGGCCCCAGGGGTCACCATGTGCACCAGGGGCCGTGAGGGGCCGGGGCATTCAGCTCAGCTCTGAACCGGGGAAGCTGGCACGGCAAGGACTGCCTCAGGTGACGGGCCGTGAGAGGGGACGGGTCAGGAGCCTTCCCAAGCCTTCTCCTCAGCCCGACACCCATGGCCATCGGAGGCTAGGATGCCAGACACAGCCATTTGCAGAAATCAGGCACAGTGACTGCAGCTCACGTCCAGCCAACCAAGCATGGGGCCGCAGCTCAGGAAGTCCCTTCCCGCCACACCACAGCCTAATTCTTACTGGGACGGAGGCAACTCGGCTACGCTGGGCAGGACGACAAACACGAGACGCCACTGTGGAATGAGCAACTTCGGAGCACGGGGTGACTTGCTTGGGACCGTGCCCACGTGACAGCCCCTTATGCAGAGGAGGAAAGAGAAGCCCCGAGTGGGAGGGGAACCTGTCCAAAGTCACACGGTGTGTGGGTGACACAGCTGGGGTGAGTCGAGGCTGGCCCCTGAGGCCCATGCTCCCTGAACGCTGGAGACCACTGTCGGCTAGCCGCGGCTCTCAGGGAAGGCCTGGTCTCCACCCTCCCAGCCTAGCCTCGCGGACCCTCGTCCTCCCCACATCGGACCTGCTCACCTGCCTGGACCCTGGGCTGCCAGATGCAGGAAGCATCAAACCCCCCAGCCTCGTGGGTGCGGGGCAGGGCGCAGGCAGCACAGCTTAGATGCCCTGGTTTGTCCCTCTTGTCTCCTGGGAAGAGCTTGCTCCCGCCCAGCTCTCCTGCCACTGGCCTTTCAGGGTTGGGCTGGGCCCAGAGTGCCTTTTAGTCGCTTCTCACGGTGGCCTGATGGCTCAACCCAGTCCCAAACGGGCCCAGTGACACTGCCGACTGCGCCCCAGCTCAGGCCCCCACTGCACCAGCAATGCTAGAAAACCAAGCCAATAAAAGTGATTTCTTTTTTCATTNOV32b, CG55906-05Protein SequenceSEQ ID NO: 7961438 aaMW at 142443.5kDMSAPDEGRRDPPKPKGKTLGSFFGSLPGFNSARNLVANAHSSARARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKQMVSGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVVQGGLDTTRSALTGTKEAVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTSAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGAMNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTGAAVNVAKGAVQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANVAXGAVQTGVDTAKTVLTGTKDTVCSGVTGAVVAKGAVQTGLKTTQNIATGTKNTLGSGVTGAAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGMDTTKTVLTGTKDAVCSGVTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDTVSTGLTGAVNLAKGTVQTGDVTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTALSPQEAPFSGISTPPDVLSVGPEPAVVEAATTKGLATDVATFTQGAAPGREDTGLLTTTHGPEEAPRLAMLQNELEGLDIFHPMNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGPSFRQRAFEHAVSHLQHGAQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32c, CG55906-01SEQ ID NO: 7973839 bpDNA SequenceORF Start: ATG at 131ORF Stop: TAG at 3806GTGAGGCCAGGCCTGCAGGTGGGTGTCGGGCTGCTCAGGCTTTCAGTGGGGAGTGGGTCTGGGATGGGAGGCTAGGGAACCCCCATTCACGCACCTTCTCTGCCCCCTTCCAGCTTCTCACGTTCTCACTATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGCCCCCCGCCCCCATGCAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGTCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTAAGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCAAGGAGGTGGTGTCCAGCGGGGTCACAGGGGCCATGGACATGGCTAAGGGGGCCGTCCAAGGGGGTCTGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCACCAAGACTGTCCTAACTGGCACCAAGAACACTGTCTGCAGTGGGGTGACCGGTGCCGTGAACTTGGCCAAAGAGGCCATCCAGGGGGGCCTGGATACCACCAAGTCTATGGTCATGGGTACGAAAGACACGATGTCCACTGGGCTCACAGGGGCAGCGAATGTGGCCAAGGGGGCCATGCAAACTGGGCTGAACACAACCCAAAATATCGCAACAGGTACAAAGGACACCGTCTGCAGTGGGGTGACTGGTGCCATGAATTTGGCCAGAGGAACCATCCAGACAGGCGTGGACACCACCAAGATCGTTCTAACTGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGCGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACGTGGCCAAAGGGACCGTCCAGACCGGCGTAGACACCACCAAGACTGTCCTAACCGGCACCAAGGACACCGTCTGCAGTGGGGTGACCAGTGCTGTGAACGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGTCTGTGGTCATAGGTACAAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCTGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTGACTACTGGGCTCGTGGGGGCAGTGAATGTCGCCAAAGGGACCGTCCAGACAGGCATGGACACCACCAAAACTGTCCTAACCGGTACCAAGGACACCATCTACAGTGGGGTCACCAGTGCCGTGAACGTGGCCAAGGGGGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCGACAGGTACAAAGAACACCTTTGGCAGTGGGGTGACCAGTGCTGTGAATGTGGCCAAAGGGGCTGCCCAGACAGGTGTAGACACGGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACTGTCCAGACCAGTGTGGACACCACCAAGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAAGGGGCCATCCAAGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAAGTTGGCCAAAGGGACTGTCCAGACCGGCATGGACACCACCAAGACTGTGTTAACTGGTACCAAGGATGCTGTGTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGATGGGTGTAGACACGGCCAAGACCGTGCTGACCGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAACGTGGCCAAGGGTGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCAACAGGTACAAAGAACACCCTTGGCAGTGGGGTGACCGGTGCTGCGAAAGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCCGTGTCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCGGTACCAAGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGGACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGACTACTGGAGTCACGGGGGCAGTGAATGTGGCCAAAGGAACCGTGCAGACCGGCGTGGACGCCTCCAAGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGACAGTGCTGACCGGAACCAAAGACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGCTCAGCTGGCTGCCTCCCAGCCTGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTACTTCGTTCGTTTAGGTGACCTGGGTCCCAGCTTCCGCCAGCGGGCATTTGAACACGCGGTGAGCCACCTGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGCCCAGCTCCAGGACTGCTTCAGGCTGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGCTGTAGGAGCCTGCAGGCCCGGCGCGGGGTCNOV32c, CG55906-01Protein SequenceSEQ ID NO: 7981225 aaMW at 120831.1kDMSAPDEGRPDPPKPKGKPPAPMQTLGSFFGSLPGFSSARNLVANAHSSVGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVVQGGLDTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGAMNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVNVAKGAAQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANVAKGAIQGGLDTTKSVLTGTKDAVSTGLTGAVKVAKGTVQTGMDTTKTVLTGTKDAVCSGVTGAANVAKGAVQMGVDTAXTVLTGTKDTVCSGVTGAANVAKGAVQTGLKTTQNIATGTKNTLGSGVTGAAKVAXGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTAQLAASQPGPKVLSAEQGSYFVRRGDLGPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQERVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32d, 277901794SEQ ID NO: 7992247 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAGATCTATCATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGGTGGCTGCCCACCCAGAGCAGACGGCCCCATGGACGGAGAAGGAGCTGCAACCTTCGGAAAAGATGGTGTCCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTACGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCAAGGAGGCGGTGTCCAGCGGGTTCACAGGGGCCATGGACATGGCTAAGGGGGCCGTCCAAGGGGGTCTGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACCAGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCGGTACCTTGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGCACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGACAGTGCTGACCGGAACCAAAGACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGACAATGGTGGGGAGCAGACTGCCCTGAGCCCCCAAGAGGCCCCGTTCTCTGGCATCTCCACGCCCCCGGATGTGCTCAGTGTAGGCCCGGAGCCTGCCTGGGAAGCCGCAGCCACTACCAAGGGCCTTGCGACTGACGTGGCGACGTTCACCCAAGGGGCCGCCCCAGGCAGGGAGGACACGGGGCTTTTGACCACCACACACGGCCCCGAAGAAGCCCCACGCTTGGCAATGCTGCAGAATGAGTTGGAGGGGCTGGGGGACATCTTCCACCCCATGAATGCGGAGGAGCAAGCTCAGCTGGCTGCCTCCCAGCCCGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTATGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGCCCAGCTCCAGGACTGCTTCAGGCTGATTGAATGCTGTCCAGGAGGAGCGGGATGCCGGGGTTCTGTCCAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGCTCGAGNOV32d, 277901794Protein SequenceSEQ ID NO: 800749 aaMW at 75993.5kDRSIMSAPDEGRRDPPKPKGKTLGSFFGSLPGFSSARNLVANAHSSARARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKMVSGAKDLVCSKMSRAKDAVSSGVASVVDVATGVVQGGLDTTRSALTGTKEAVSSGFTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTSAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTALSPQEAPFSGISTPPDVLSVGPEPAWEAAATTKGLATDVATFTQGAAPGREDTGLLTTTHGPEEAPRLAMLQNELEGLGDIFHPNNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQLENOV32e, CG55906-02SEQ ID NO: 801810 bpDNA SequenceORF Start: ATG at 123ORF Stop: TAG at 792AGGCCTGCAGGTGGGTGTCGGGCTGCTCAGGCTTTCAGTGGGGAGTGGGTGTGGGATGGGAGGCTAGGGAACCCCCATTCACGCACCTTCTCTGCCCCCTTCCAGCTTCTCACGTTCTCACTATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAACTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGCGAGAGCCCGGCCGGCCGCTGACCCCACAGGAGCGCCTGCTGCCGAGGCTGCCCAACCACAGGCTCAGGTGGCTGCCCACCCAGAGCAGACGGCCCCATGGACGGAGAAGGAGCTGCAACCTTCGGAAAAGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGGAGCGGGATGCCGGGGTTCTGTCCAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCGGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGCTGTAGGAGCCTGCAGNOV32e, CG55906-02Protein SequenceSEQ ID NO: 802223 aaMW at 23486.9kDMSAPDEGRRDPPKPKGKTLGSFFGSLPGFNSARNLVANAHSSARARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKIEKAQQAPEGQPRLDQGSGASAEDAAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLRGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32f, 230272941SEQ ID NO: 803714 bpDNA SequenceORF Start: at 2ORF Stop: TGA at 692GGCGCGCCCACCCTTCACCACCATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGCGAGAGCCCGGCCGGCCGCTGACCCCACAGGAGCGCCTGCTGCCGAGGCTGCCCAACCACAGGCTCAGGTGGCTGCCCACCCAGAGCAGACGGCCCCATGGACGGAGAAGGAGCTGCAACCTTCGGAAAAGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGGAGCGGGATGCCGGGGTTCTGTCCAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTGAAGGGGGCGGCCGCGGAGCCTNOV32f, 230272941Protein SequenceSEQ ID NO: 804230 aaMW at 24202.7kDARPPFTTMSAPDEGRRDPPKPKGKTLGSFFGSLPGFSSARNLVANAHSSAPARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKIEKAQQAPEGQPRLDQGSGASAEDAAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32g, CG55906-03SEQ ID NO: 805714 bpDNA SequenceORF Start: ATG at 23ORF Stop: TGA at 692GGCGCGCCCACCCTTCACCACCATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGCGAGAGCCCGGCCGGCCGCTGACCCCACAGGAGCGCCTGCTGCCGAGGCTGCCCAACCACAGGCTCAGGTGGCTGCCCACCCAGAGCAGACGGCCCCATGGACGGAGAAGGAGCTGCAACCTTCGGAAAAGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGGAGCGGGATGCCGGGGTTCTGTCCAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTGAAGGGGGCGGCCGCGGAGCCTNOV32g, CG55906-03SequenceSEQ ID NO: 806223 aaMW at 23431.8kDMSAPDEGRRDPPKPKGKTLGSFFGSLPGFSSARNLVANAHSSARARPAADPTGAPAAEAAQPQAQVAAHPEQTAPWTEKELQPSEKIEKAQQAPEGQPRLDQGSGASAEDAAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32h,SNP13382505 ofSEQ ID NO: 8073839 bpCG55906-01,ORF Start: ATG at 131ORF Stop: TAG at 3806DNA SequenceSNP Pos: 3393SNP Change: C to TGTGAGGCCAGGCCTGCAGGTGGGTGTCGGGCTGCTCAGGCTTTCAGTGGGGAGTGGGTGTGGGATGGCAGGCTAGGGAACCCCCATTCACGCACCTTCTCTGCCCCCTTCCAGCTTCTCACGTTCTCACTATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGCCCCCCGCCCCCATGCAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGTCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTAAGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCAAGGAGGTGGTGTCCAGCGGGGTCACAGGGGCCATGGACATGGCTAAGGGGGCCGTCCAAGGGGGTCTGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCACCAAGACTGTCCTAACTGGCACCAAGAACACTGTCTGCAGTGGGGTGACCGGTGCCGTGAACTTGGCCAAAGAGGCCATCCAGGGGGGCCTGGATACCACCAAGTCTATGGTCATGGGTACGAAAGACACGATGTCCACGGGACTCACAGGGGCAGCGAATGTGGCCAAGGGGGCCATGCAAACTGGGCTGAACACAACCCAAAATATCGCAACAGGTACAAAGGACACCGTCTGCAGTGGGGTGACTGGTGCCATGAATTTGGCCAGAGGAACCATCCAGACAGGCGTGGACACCACCAAGATCGTTCTAACTGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGCGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACGTGGCCAAAGGGACCGTCCAGACCGGCGTAGACACCACCAAGACTGTCCTAACCGGCACCAAGGACACCGTCTGCAGTGGGGTGACCAGTGCTGTGAACGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGTCTGTGGTCATAGGTACAAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCTGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTGACTACTGGGCTCGTGGGGGCAGTGAATGTCGCCAAAGGGACCGTCCAGACAGGCATGGACACCACCAAAACTGTCCTAACCGGTACCAAGGACGACCCAAAATATCGCGACAGGTACAAAGAACACCTTTGGCAGTGGGGTGACCAGTGCTGTGAATGTGGCCAAAGGGGCTGCCCAGACAGGTGTAGACACGGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACTGTCCAGACCAGTGTGGACACCACCAAGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAAGGGGCCATCCAAGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAAGTTGGCCAAAGGGACTGTCCAGACCGGCATGGACACCACCAAGACTGTGTTAACTGGTACCAAGGATGCTGTGTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGATGGGTGTAGACACGGCCAAGACCGTGCTGACCGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAACGTGGCCAAGGGTGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCAACAGGTACAAAGAACACCCTTGGCAGTGGGGTGACCGGTGCTGCGAAAGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCCGTGTCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCGGTACCAAGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGGACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGACTACTGGAGTCACGGGGGCAGTGAATGTGGCCAAAGGAACCGTGCAGACCGGCGTGGACGCCTCCAAGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACC)AGACAGTGCTGACCGGAACCAAAGACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGCTCAGCTGGCTGCCTCCCAGCCTGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTACTTCGTTCGTTTAGGTGACCTGGGTCCCAGCTTCCGCCAGCGGGCATTTGAACACGCGGTGAGCCACCTGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGTCCAGCTCCAGGACTGCTTCAGGCTGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGCTGTAGGAGCCTGCAGGCCCGGCGCGGGGTCNOV32h,SNP13382505 ofCG55906-01,SEQ ID NO: 808MW at 120859.1kDProtein SequenceSNP Pos: 10881225 aaSNP Change: Ala to ValMSAPDEGRRDPPKPKGKPPAPMQTLGSFFGSLPGFSSARNLVANAHSSVGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVVQGGLDTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGANNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGAMNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAIKTVLTGTKDTVTTGLVGAVNVAKGTVQTGDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVNVAXGAAQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANVAKGAQGGLDTTKSVLTGTKDAVSTGLTGAVKLAIKGTVQTGMDTTKTVLTGTKDAVCSGVTGAANVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAANVAKGAVQTGLKTTQNIATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAXDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTAQLAASQPGPKVLSAEQGSYFVRLGDLGPSFRQRAFEHAVSHLQHGQFQARDTLVQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQERVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGSNP1 3382504 ofSEQ ID NO: 8093839 bpG55906-01,ORF Start: ATG at 131ORF Stop: TAG at 3806DNA SequenceSNP Pos: 3409SNP Change: C to TGTGAGGCCAGGCCTGCAGGTGGGTGTCGGGCTGCTCAGGCTTTCAGTGGGGAGTGGGTGTGGGATCGGAGGCTAGGGAACCCCCATTCACGCACCTTCTCTGCCCCCTTCCAGCTTCTCACGTTCTCACTATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGCCCCCCGCCCCCATGCAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGTCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTAAGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCAAGGAGGTGGTGTCCAGCGGGGTCACAGGGGCCATGGACATGGCTAAGGGGGCCGTCCAAGGGGGTCTGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCACCAAGACTGTCCTAACTGGCACCAAGAACACTGTCTGCAGTGGGGTGACCGGTGCCGTGAACTTGGCCAAAGAGGCCATCCAGGGGGGCCTGGATACCACCAAGTCTATGGTCATGGGTACGAAAGACACGATGTCCACTGGGCTCACAGGGGCAGCGAATGTGGCCAAGGGGGCCATGCAAACTGGGCTGAACACAACCCAAAATATCGCAACAGGTACAAAGGACACCGTCTGCAGTGGGGTGACTGGTGCCATGAATTTGGCCAGAGGAACCATCCAGACAGGCGTGGACACCACCAAGATCGTTCTAACTGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGCGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACGTGGCCAAAGGGACCGTCCAGACCGGCGTAGACACCACCAAGACTGTCCTAACCGGCACCAAGGACACCGTCTGCAGTGGGGTGACCAGTGCTGTGAACGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGTCTGTGGTCATAGGTACAAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCTGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTGACTACTGGGCTCGTGGGGGCAGTGAATGTCGCCAAAGGGACCGTCCAGACAGGCATGGACACCACCAAAACTGTCCTAACCGGTACCAAGGACACCATCTACAGTGGGGTCACCAGTGCCGTGAACGTGGCCAAGGGGGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCGACAGGTACAAAGAACACCTTTGGCAGTGGGGTGACCAGTGCTGTGAATGTGGCCAAAGGGGCTGCCCAGACAGGTGTAGACACGGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACTGTCCAGACCAGTGTGGACACCACCAAGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAAGGGGCCATCCAAGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAAGTTGGCCAAAGGGACTGTCCAGACCGGCATGGACACCACCAAGACTGTGTTAACTGGTACCAAGGATGCTGTGTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGATGGGTGTAGACACGGCCAAGACCGTGCTGACCGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAACGTGGCCAAGGGTGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCAACAGGTACAAAGAACACCCTTGGCAGTGGGGTGACCGGTGCTGCGAAAGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCCGTGTCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCAATACCAAGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGGACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGACTACTGGAGTCACGGGGGCAGTGAATGTGGCCAAAGGAACCGTGCAGACCGGCGTGGACGCCTCCAAGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGACAGTGCTGACCGGAACCAAAGACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGCTCAGCTGGCTGCCTCCCAGCCTGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTACTTCGTTCGTTTAGGTGACCTGGGTCCCAGCTTCCGCCAGCGGGCATTTGAACACGCGGTGAGCCACCTGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGCCCAGCTCCAGGACTGTTTCAGGCTGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCTGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGCTGTAGGAGCCTGCAGGCCCGGCGCGGGGTCNOV32i,SNP13382504 ofCG559O6-01,SEQ ID NO: 810MW at 120831.1kDProtein SequenceSNP Pos: 10931225 aaSNP Change: Cys to CysMSAPDEGRRDPPKPKGKPPAPMQTLGSFFGSLPGFSSAENLVANAHSSVGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVVQGGLDTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGANNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGANNVAXGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGAMNTARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVVAKGAAQTGVDTAKTXTLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANVAKGAIQGGLDTTKSVLTGTKDAVSTGLTGAVKLAKGTVQTGMDTTKTVLTGTKDAVCSGVTGAAAVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAAINAKGAVQTGLKTTQNIATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTAQLAASQPGPKVLSAEQGSYFVRLGDLGPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQERVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQNOV32j,SNP13376440 ofSEQ ID NO: 8113839 bpCG55906-01,ORF Start: ATG at 131ORF Stop: TAG at 3806DNA Sequence SNP Pos: 3681SNP Change: T to CGTGAGGCCAGGCCTGCAGGTGGGTGTCGGGCTGCTCAGGCTTTCAGTGGGGAGTGGGTGTGGGATGGGAGGCTAGGGAACCCCCATTCACGCACCTTCTCTGCCCCCTTCCAGCTTCTCACGTTCTCACTATGTCTGCTCCAGACGAAGGGAGACGGGATCCCCCCAAACCGAAGGGCAAGCCCCCCGCCCCCATGCAGACCCTGGGCAGCTTCTTTGGGTCCCTGCCTGGCTTCAGCTCTGCCCGGAACCTGGTGGCCAACGCACATAGCTCGGTCGGGGCCAAAGACCTGGTGTGTTCCAAGATGTCCAGGGCCAAGGATGCCGTGTCCTCCGGGGTGGCCAGCGTGGTGGACGTGGCTAAGGGAGTGGTCCAGGGAGGCCTGGACACCACTCGGTCTGCACTTACGGGCACCAAGGAGGTGGTGTCCAGCGGGGTCACAGGGGCCATGGACATGGCTAAGGGGGCCGTCCAAGGGGGTCTGGACACCTCGAAGGCTGTCCTCACCGGCACCAAGGACACGGTGTCCACTGGGCTCACGGGGGCAGTGAATGTGGCCAAAGGGACCGTACAGGCCGGTGTGGACACCACCAAGACTGTGCTGACCGGCACCAAAGACACAGTGACTACTGGGGTCATGGGGGCAGTGAACTTGGCCAAAGGGACTGTCCAGACTGGCGTGGAAACCTCCAAGGCTGTGCTGACCGGCACCAAAGATGCTGTGTCCACTGGGCTCACAGGGGCAGTGAATGTGGCCAGAGGAAGCATTCAGACCGGTGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCAGTAAGACTGTCCTAACAGGTACCAAGGACACCGTCTGTAGTGGGGTGACTGGTGCCATGAATGTGGCCAAAGGAACCATCCAGACCGGCGTGGACACCACCAAGACTGTCCTAACTGGCACCAAGAACACTGTCTGCAGTGGGGTGACCGGTGCCGTGAACTTGGCCAAAGAGGCCATCCAGGGGGGCCTGGATACCACCAAGTCTATGGTCATGGGTACGAAAGACACGATGTCCACTGGGCTCACAGGGGCAGCGAATGTGGCCAAGGGGGCCATGCAAACTGGGCTGAACACAACCCAAAATATCGCAACAGGTACAAAGGACACCGTCTGCAGTGGGGTGACTGGTGCCATGAATTTGGCCAGAGGAACCATCCAGACAGGCGTGGACACCACCAAGATCGTTCTAACTGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAATGTGGCCAAAGGGGCCGTCCAGGGCGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAACGTGGCCAAAGGGACCGTCCAGACCGGCGTAGACACCACCAAGACTGTCCTAACCGGCACCAAGGACACCGTCTGCAGTGGGGTGACCAGTGCTGTGAACGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGTCTGTGGTCATAGGTACAAAAGACACGATGTCCACTGGGCTCACGGGGGCAGCGAATGTGGCCAAGGGGGCTGTCCAGACAGGTGTAGACACAGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTGACTACTGGGCTCGTGGGGGCAGTGAATGTCGCCAAAGGGACCGTCCAGACAGGCATGGACACCACCAAAACTGTCCTAACCGGTACCAAGGACACCATCTACAGTGGGGTCACCAGTGCCGTGAACGTGGCCAAGGGGGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCGACAGGTACAAAGAACACCTTTGGCAGTGGGGTGACCAGTGCTGTGAATGTGGCCAAAGGGGCTGCCCAGACAGGTGTAGACACGGCCAAGACCGTGCTGACCGGCACCAAGGACACAGTCACTACTGGGCTCATGGGGGCAGTGAATGTCGCCAAAGGGACTGTCCAGACCAGTGTGGACACCACCATGACTGTCCTAACTGGTACCAAGGACACCGTCTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAAGGGGCCATCCAAGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCTGTGTCCACTGGGCTCACAGGGGCTGTGAAGTTGGCCAAAGGGACTGTCCAGACCGGCATGGACACCACCAAGACTGTGTTAACTGGTACCAAGGATGCTGTGTGCAGTGGGGTGACCGGTGCTGCGAATGTGGCCAAGGGGGCCGTCCAGATGGGTGTAGACACGGCCAAGACCGTGCTGACCGGTACCAAGGACACTGTCTGCAGTGGGGTCACCGGTGCTGCGAACGTGGCCAAGGGTGCTGTGCAAACTGGGCTGAAAACGACCCAAAATATCGCAACAGGTACAAAGAACACCCTTGGCAGTGGGGTGACCGGTGCTGCGAAAGTGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACTACAAAGTCTGTCCTGACTGGCACTAAAGATGCCGTGTCCACTGGGCTCACAGGGGCTGTGAACTTGGCCAAAGGGACTGTCCAGACCGGCGTGGACACCAGCAAGACTGTCCTGACCGGTACCAAGGACACCGTCTGCAGTGGAGTCACTGGTGCCGTAAATGTGGCCAAAGGGACCGTCCAGACAGGTGTGGACACAGCCAAGACGGTGCTGAGTGGCGCTAAGGATGCAGTGACTACTGGAGTCACGGGGGCAGTGAATGTGGCCAAAGGAACCGTGCAGACCGGCGTGGACGCCTCCAAGGCTGTGCTTATGGGTACCAAGGACACTGTCTTCAGTGGGGTTACCGGTGCCATGAGCATGGCCAAAGGGGCCGTCCAGGGGGGCCTGGACACCACCAAGACAGTGCTGACCGGAACCAAAGACGCAGTGTCCGCTGGGCTCATGGGGTCAGGGAACGTGGCGACAGGGGCCACCCACACTGGCCTCAGCACCTTCCAGAACTGGTTACCTAGTACCCCCGCCACCTCCTGGGGTGGACTCACCAGTTCCAGGACCACAGCTCAGCTGGCTGCCTCCCAGCCTGGGCCAAAGGTGCTGTCGGCGGAACAGGGGAGCTACTTCGTTCGTTTAGGTGACCTGGGTCCCAGCTTCCGCCAGCGGGCATTTGAACACGCGGTGAGCCACCTGCAGCACGGCCAGTTCCAAGCCAGGGACACTCTGGCCCAGCTCCAGGACTGTTTCAGGCTGATTGAAAAGGCCCAGCAGGCTCCAGAAGGGCAGCCACGTCTGGACCAGGGCTCAGGTGCCAGTGCGGAGGACGCTGCTGTCCAGGAGAGGGTCTGCGGCCTTCTCCGGCAGCTGCACACGGCCTACAGTGGCCTGGTCTCCAGCCTCCAGGGCCTGCCCGCCGAGCTCCAGCAGCCAGTGGGGCGGGCGCGGCACAGCCTCTGTGAGCTCTATGGCATCGTGGCCTCAGCTGGCTCTGTAGAGGAGCTGCCCGCAGAGCGGCCGGTGCAGAGCCGCGAGGGTGTGCACCAGGCTTGGCAGGGGTTAGAGCAGCTGCTGGAGGGCCTACAGCACAATCCCCCGCTCAGCTGGCTGGTAGGGCCCTTCGCCTTGCCCGCTGGCGGGCAGTAGCTGTAGGAGCCTGCAGGCCCGGCGCGGGGTCNOV32j,SNP13376440 ofCG55906-01,SEQ ID NO: 812MW at 120815.0kDProtein SequenceSNP Pos: 11841225 aaSNP Change: Leu to ProMSAPDEGRPDPPKPKGKPPAPMQTLGSFFGSLPGFSSARNLVANAHSSVGAKDLVCSKMSRAKDAVSSGVASVVDVAXGVVQGGLDTTRSALTGTKEVVSSGVTGANDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTTCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGTKDTVCSGVTGANNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAXGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVNVAKGAAQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANVAKGATQGGLDTTKSVLTGTKDAVSTGLTGAVKLAKGTVQTGMDTTKTVLTGTKDAVCSGVTGAANVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAANVAKGAVQTGLKTTQNIATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTAQLAASQPGPKTLSAEQGSYFVRLGDLGPSFRQPAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDAAVQERVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASAGSVEELPAERPVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 32B.

TABLE 32BComparison of the NOV32 protein sequences.NOV32a------------------------------------------------------------(SEQ ID NO:794)NOV32bMSAPDEGRRDPPKPKGKTLGSFFGSLPGFNSARNLVANAHSSARARPAADPTGAPAAEAA(SEQ ID NO:796)NOV32c------------------------------------------------------------(SEQ ID NO:798)NOV32d------------------------------------------------------------(SEQ ID NO:800)NOV32e------------------------------------------------------------(SEQ ID NO:802)NOV32f------------------------------------------------------------(SEQ ID NO:804)NOV32g------------------------------------------------------------(SEQ ID NO:806)NOV32a------------------------------------------------------------NOV32bQPQAQVAAHPEQTAPWTEKELQPSEKQMVSGAKDLVCSKNSRAKDAVSSGVASVVDVAKGNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bVVQGGLDTTRSALTGTKEAVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGANOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bVNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bTGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTSAMNVAKGTIQTGVDTSKTVLTGNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bTGLNTTQNIATGTKDTVCSGVTGANNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bTSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDNOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a------------------------------------------------------------NOV32bTVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNINOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32a----------------------------------MSAPDEGRRDPPKPKGK------TLGNOV32bATGTKNTFGSGVTGAVNVAKGAVQTGVDTAKTVLTGTKDTVTTGLMGAVNV------AKGNOV32c----------------------------------MSAPDEGRPDPPKPKGKPPAPMQTLGNOV32d-------------------------------RSIMSAPDEGRRDPPKPKGK------TLGNOV32e----------------------------------MSAPDEGRRDPPKPKGK------TLGNOV32f---------------------------ARPPFTTMSAPDEGRPDPPKPKGK------TLGNOV32g----------------------------------MSAPDEGRRDPPKPKGK------TLGNOV32aSFFGSLPGFSSARNLVANAHSS-----------ARARPAADPTGAPAAEAAQPQAQVAAHNOV32bTVQTSVDTTKTVLTGTKDTVCS-----------GVTGAANVAKGAVQTGVDTAKTVLTGTNOV32cSFFGSLPGFSSAPNLVANAHSSVGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVVQGGLDNOV32dSFFGSLPGFSSARNLVANAHSS-----------ARARPAADPTGAPAAEAAQPQAQVAAHNOV32eSFFGSLPGFNSARNLVANAHSS-----------ARARPAADPTGAPAAEAAQPQAQVAAHNOV32fSFFGSLPGFSSARNLVANAHSS-----------ARARPAADPTGAPAAEAAQPQAQVAAHNOV32gSFFGSLPGFSSAPNLVANAHSS-----------ARARPAADPTGAPAAEAAQPQAQVAAHNOV32aPEQTAPWTEKELQPSEKQ--------MVSGAKDLVCSKMSRAKDAVSSGVASVVDVAKGVNOV32bKDTVCSGVTGAVNVAKG---------AVQTGLKTTQNIATGTKNTLGSGVTGAANVAKGANOV32cTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNVAKGTNOV32dPEQTAPWTEKELQPSEK---------MVSGAKDLVCSKMSRAYDAVSSGVASVVDVATGVNOV32ePEQTAPWTEKELQPSE--------------------------------------------NOV32fPEQTAPWTEKELQPSE--------------------------------------------NOV32gPEQTAPWTEKELQPSE--------------------------------------------NOV32aVQGGLDTTRSALTGTKEVVSSGVTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNOV32bVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGMDTTKTVLTGTKDAVCSGVTGAANOV32cVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAXGTVQTGVETSKAVLTGTKDAVSTGLTGAVNOV32dVQGGLDTTRSALTGTKEAVSSGFTGAMDMAKGAVQGGLDTSKAVLTGTKDTVSTGLTGAVNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTNOV32bNVAKGAVQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSNOV32cNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSNOV32dNVAKGTVQAGVDTTKTVLTGTKDTVTTGVMGAVNLAKGTVQTGVETSKAVLTGTKDAVSTNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTNOV32bGVTGAANWAKGAVQGGLDTTKSVLTGTKDTVSTGLTGAVNLAKGTVQTGVDTSKTVLTGTNOV32cGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTNOV32dGLTGAVNVARGSIQTGVDTSKTVLTGTKDTVCSGVTSAMNNAKGTIQTGVDTSKTVLTGTNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aKDTVCSGVTGAMNVAKGTIQTGVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKNOV32bKDTVCSGVT---------------------------------------------------NOV32cKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNOV32dKDTVCSGVT---------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTNOV32b------------------------------------------------------------NOV32cNIATGTKDTVCSGVTGAMNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVAKGAVQGNOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aGLNTTQNIATGTKDTVCSGVTGAMNLARGTIQTGVDTTKIVLTGTKDTVCSGVTGAANVANOV32b------------------------------------------------------------NOV32cG---LDTTKSVLTG----------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTNOV32b------------------------------------------------------------NOV32c------------------------------------------------------------NOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aSAVNVAKGAVQGGLDTTKSVVIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTNOV32b------------------------------------------------------------NOV32c--------------------------------------------------------TKDANOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aVTTGLVGAVNVAKGTVQTGMDTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIANOV32b------------------------------------------------------------NOV32cVSTGLTGAVNVAKGTVQTGVDTTKTVLTGTKDTVCSGVTSAVNVAKGAVQGGLDTTKSVVNOV32d------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aTGTKNTFGSGVTSAVNVAKGAAQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDNOV32b------------------------------------------------------------NOV32cIGTKDTMSTGLTGAANVAKGAVQTGVDTAKTVLTGTKDTVTTGLVGAVNVAKGTVQTGMDNOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aTTKTVLTGTKDTVCSGVTGAANVAKGAIQGGLDTTKSVLTGTKDAVSTGLTGAVKLAKGTNOV32b------------------------------------------------------------NOV32cTTKTVLTGTKDTIYSGVTSAVNVAKGAVQTGLKTTQNIATGTKNTFGSGVTSAVNVAKGANOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aVQTGMDTTKTVLTGTKDAVCSGVTGAANVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAANOV32b------------------------------------------------------------NOV32cAQTGVDTAKTVLTGTKDTVTTGLMGAVNVAKGTVQTSVDTTKTVLTGTKDTVCSGVTGAANOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aNVAKGAVQTGLKTTQNTATGTKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTNOV32b------------------------------------------------------------NOV32cNVAKGAIQGGLDTTKSVLTGTKDAVSTGLTGAVKLAKGTVQTGMDTTKTVLTGTKDAVCSNOV32d------------------------------------------------------------NOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aGLTGAVNLAKGTVQTGVDTSKTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGANOV32b------------------------------------GAVNVAKGTVQTGVDTAKTVLSGANOV32cGVTGAANVAKGAVQMGVDTAKTVLTGTKDTVCSGVTGAANVAKGAVQTGLKTTQNIATGTNOV32d------------------------------------GAVNVAKGTVQTGVDTAKTVLSGANOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKNOV32bKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKNOV32cKNTLGSGVTGAAKVAKGAVQGGLDTTKSVLTGTKDAVSTGLTGAVNLAKGTVQTGVDTSKNOV32dKDAVTTGVTGAVNVAKGTVQTGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32aTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTNOV32bTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTNOV32cTVLTGTKDTVCSGVTGAVNVAKGTVQTGVDTAKTVLSGAKDAVTTGVTGAVNVAKGTVQTNOV32dTVLTGTKDAVSAGLMGSGNVATGATHTGLSTFQNWLPSTPATSWGGLTSSRTTDNGGEQTNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aALSPQEAPFSGISTPPDVLSVGPEPAWEAAATTKGLATDVATFTQGAAPGREDTGL-LATNOV32bALSPQEAPFSGISTPPDVLSVGPEPAWEAAATTKGLATDVATFTQGAAPGREDTGL-LTTNOV32cGVDASKAVLMGTKDTVFSGVTGAMSMAKGAVQGGLDTTKTVLTGTKDAVSAGLMGSGNVANOV32dALSPQEAPFSGISTPPDVLSVGPEPAWEAAATTKGLATDVATFTQGAAPGREDTGL-LTTNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aTHGPEEAPRLAMLQNELEGLGDTFHPNNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGNOV32bTHGPEEAPRLAMLQNELEGLGDIFHPNNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGNOV32cTGATHTGLSTFQNWLPSTPATSWGGLTSSRTTAQLAASQPGPKVLSAEQGSYFVRLGDLGNOV32dTHGPEEAPRLAMLQNELEGLGDIFHPMNAEEQAQLAASQPGPKVLSAEQGSYFVRLGDLGNOV32e------------------------------------------------------------NOV32f------------------------------------------------------------NOV32g------------------------------------------------------------NOV32aPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDANOV32bPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDANOV32cPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDANOV32dPSFRQRAFEHAVSHLQHGQFQARDTLAQLQDCFRLIEKAQQAPEGQPRLDQGSGASAEDANOV32e----------------------------------KIEKAQQAPEGQPRLDQGSGASAEDANOV32f----------------------------------KIEKAQQAPEGQPRLDQGSGASAEDANOV32g----------------------------------KIEKAQQAPEGQPRLDQGSGASAEDANOV32aAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASANOV32bAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASANOV32cAVQER--------VCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASANOV32dAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASANOV32eAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLRGLPAELQQPVGRARHSLCELYGIVASANOV32fAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASANOV32gAVQEERDAGVLSRVCGLLRQLHTAYSGLVSSLQGLPAELQQPVGRARHSLCELYGIVASANOV32aGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ--NOV32bGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ--NOV32cGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ--NOV32dGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQLENOV32eGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ--NOV32fGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ--NOV32gGSVEELPAERLVQSREGVHQAWQGLEQLLEGLQHNPPLSWLVGPFALPAGGQ--


Further analysis of the NOV32a protein yielded the following properties shown in Table 32C.

TABLE 32CProtein Sequence Properties NOV32aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 2; neg. chg 3H-region:length 2; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −4.76possible cleavage site: between 32 and 33>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 1.43 (at 106)ALOM score: 1.43 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):6.59Hyd Moment(95):5.57G content:0D/E content:2S/T content:1Score: −6.74Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.6%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):56.5%: cytoplasmic26.1%: nuclear13.0%: mitochondrial 4.3%: vesicles of secretory system>> prediction for CG55906-04 is cyt (k = 23)


A search of the NOV32a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 32D.

TABLE 32DGeneseq Results for NOV32aNOV32aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAO21705Human secreted protein SEQ ID No1 . . . 13721297/1372(94%)0.047 - Homo sapiens, 1325 aa.1 . . . 13251306/1372(94%)[WO200238602-A2, 16-MAY-2002]ABB12254Human S3-12 homologue, SEQ ID1 . . . 12351234/1235(99%)0.0NO: 2624 - Homo sapiens, 1250 aa.17 . . . 1250 1234/1235(99%)[WO200157188-A2, 09-AUG-2001]ABP53597Human NOV19 protein SEQ ID1 . . . 10741026/1080(95%)0.0NO: 58 - Homo sapiens, 1225 aa.1 . . . 10331026/1080(95%)[WO200262999-A2, 15-AUG-2002]AAY95851Autoantigen diagnostic of314 . . . 749  431/436(98%)0.0endometriosis - Homo sapiens, 4394 . . . 439 432/436(98%)aa. [WO200047739-A2, 17-AUG-2000]AAY48492Human breast tumour-associated1049 . . . 1372  323/324(99%)0.0protein 37 - Homo sapiens, 324 aa.1 . . . 324 323/324(99%)[DE19813835-A1, 23-SEP-1999]


In a BLAST search of public sequence databases, the NOV32a protein was found to have homology to the proteins shown in the BLASTP data in Table 32E.

TABLE 32EPublic BLASTP Results for NOV32aNOV32aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96Q06Hypothetical protein KIAA1881 - 91 . . . 13721256/1348(93%)0.0Homo sapiens (Human), 1348 aa 1 . . . 13481264/1348(93%)(fragment).O88492S3-12 - Mus musculus (Mouse), 1 . . . 1370843/1432(58%)0.01403 aa. 1 . . . 14011043/1432(71%)Q98MG7Hypothetical glycine-rich protein141 . . . 1141352/1088(32%)5e−71mlr0587 - Rhizobium loti1078 . . . 2144 387/1088(35%)(Mesorhizobium loti), 3145 aa.Q98MG8Hypothetical glycine-rich protein128 . . . 1141325/1070(30%)8e−63mlr0585 - Rhizobium loti329 . . . 1379384/1070(35%)(Mesorhizobium loti), 2147 aa.Q96WU8Hypothetical 119.8 kDa protein -140 . . . 1088265/996(26%)4e−42Schizosaccharomyces pombe266 . . . 1173348/996(34%)(Fission yeast), 1195 aa (fragment).


PFam analysis predicts that the NOV32a protein contains the domains shown in the Table 32F.

TABLE 32FDomain Analysis of NOV32aIdentities/PfamNOV32aSimilarities forExpectDomainMatch Regionthe Matched RegionValueLEA 87 . . . 15616/75(21%)0.0007644/75(59%)LEA516 . . . 58915/76(20%)0.9646/76(61%)perilipin 592 . . . 135689/788(11%)0.045435/788(55%)


Example 33

The NOV33 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 33A.

TABLE 33ANOV33a,CG55908-01SEQ ID NO:813840 bpDNA SequenceORF Start: at 2ORF Stop: TAA at 836GGCCTCCGGGATTTGCTACCTTTTTGGCTCCCTGCTCGTCGAACTGCTCTTCTCACGGGCTGTCGCCTTCAATCTGGACGTGATGGGTGCCTTGCGCAAGGAGGGCGAGCCAGGCAGCCTCTTCGGCTTCTCTGTGGCCCTGCACCGGCAGTTGCAGCCCCGACCCCAGAGCTGGCTGCTGGTGGGTGCTCCCCAGGCCCTGGCTCTTCCTGGGCAGCAGGCGAATCGCACTGGAGGCCTCTTCGCTTGCCCGTTGAGCCTGGAGGAGACTGACTGCTACAGAGTGGACATCGACCAGGGAGCTGATATGCAAAAGGAAAGCAAGGAGAACCAGTGGTTGGGAGTCAGTGTTCGGAGCCAGGGGCCTGGGGGCAAGATTGTTGACTGCGCCCGGGGCACGGCCAACTGTGTGGTGTTCAGCTGCCCACTCTACAGCTTTGACCGCGCGGCTGTGCTGCATGTCTGGGGCCGTCTCTGGAACAGCACCTTTCTGGAGGAGTACTCAGCTGTGAAGTCCCTGGAAGTGATTGTCCGGGCCAACATCACAGTGAAGTCCTCCATAAAGAACTTGATGCTCCGAGATGCCTCCACAGTGATCCCAGTGATGGTATACTTGGACCCCATGGCTGTGGTGGCAGAAGGAGTGCCCTGGTGGGTCATCCTCCTGGCTGTACTGGCTGGGCTGCTGGTGCTAGCACTGCTGGTGCTGCTCCTGTGGAAGTGTGGCTTCTTCCATCGGAGCAGCCAGAGCTCATCTTTTCCCACCAACTATCACCGGGCCTGTCTGGCTGTGCAGCCTTCAGCCATGGAAGTTGGGGGTCCAGGGACTGTGGGGTAACTNOV33a, CG55908-01SEQ ID NO:814278 aaMW at 30129.7 kDProtein SequenceASGTCYLFGSLLVELLFSRAVAFNLDVMGALRKEGEPGSLFGFSVALHRQLQPRPQSWLLVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQGPGGKIVDCARGTANCVVFSCPLYSFDPAAVLHVWGRLWNSTFLEEYSAVKSLEVIVRANITVKSSIKNLMLRDASTVIPVMVYLDPMAVVAEGVPWWVILLAVLAGLLVLALLVLLLWKCGFFHRSSQSSSFPTNYHRACLAVQPSAMEVGGPGTVGNOV33b, 253116407SEQ ID NO:815780 bpDNA SequenceORF Start: at 1ORE Stop: end of sequenceGGATCCTTCAATCTGGACGTGATGGGTGCCTTGCGCAAGGAGGGCGAGCCAGGCAGCCTCTTCGGCTTCTCTGTGGCCCTGCACCGGCAGTTGCAGCCCCGACCCCAGAGCTGGCTGCTGGTGGGTGCTCCCCAGGCCCTGGCTCTTCCTGGGCAGCAGGCGAATCGCACTGGAGGCCTCTTCGCTTGCCCGTTGAGCCTGGAGGAGACTGACTGCTACAGAGTGGACATCGACCAGGGAGCTGATATGCAAAAGGAAAGCAAGGAGAACCAGTGGTTGGGAGTCAGTGTTCGGAGCCAGGGGCCTGGGGGCAAGATTGTTGACTGCGCCCGGGGCACGGCCAACTGTGTGGTGTTCAGCTGCCCACTCTACAGCTTTGACCGCGCGGCTGTGCTGCATGTCTGGGGCCGTCTCTGGAACAGCACCTTTCTGGAGGAGTACTCAGCTGTGAAGTCCCTGGAAGTGATTGTCCGGGCCAACATCACAGTGAAGTCCTCCATAAAGAACTTGATGCTCCGAGATGCCTCCACAGTGATCCCAGTGATGGTATACTTGGACCCCATGGCTGTGGTGGCAGAAGGAGTGCCCTGGTGGGTCATCCTCCTGGCTGTACTGGCTGGGCTGCTGGTGCTAGCACTGCTGGTGCTGCTCCTGTGGAAGTGTGGCTTCTTCCATCGGAGCAGCCAGAGCTCATCTTTTCCCACCAACTATCACCGGGCCTGTCTGGCTGTGCAGCCTTCAGCCATGGAAGTTGGGGGTCCAGGGACTGTGGGGCTCGAGNOV33b, 253116407SEQ ID NO:816260 aaMW at 28204.4 kDProtein SequenceGSFNLDVMGALRKEGEPGSLFGFSVALHRQLQPRPQSWLLVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQGPGGKIVDCARGTANCVVFSCPLYSFDRAAVLHVWGRLWNSTFLEEYSAVKSLEVIVRANITVKSSIKNLMLRDASTVIPVMVYLDPMAVVAEGVPWWVILLAVLAGLLVLALLVLLLWKCGFFHRSSQSSSFPTNYHRACLAVQPSAMEVGGPGTVGLENOV33c, 253116412SEQ ID NO:817567 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCTTCAATCTGGACGTGATGGGTGCCTTGCGCAAGGAGGGCGAGCCAGGCAGCCTCTTCGGCTTCTCTGTGGCCCTGCACCGGCAGTTGCAGCCCCGACCCCAGAGCTGGCTGCTGGTGGGTGCTCCCCAGGCCCTGGCTCTTCCTGGGCAGCAGGCGAATCGCACTGGAGGCCTCTTCGCTTGCCCGTTGAGCCTGGAGGAGACTGACTGCTACAGAGTGGACATCGACCAGGGAGCTGATATGCAAAAGGAAAGCAAGGAGAACCAGTGGTTGGGAGTCAGTGTTCGGAGCCAGGGGCCTGGGGGCAAGATTGTTGACTGCGCCCGGGGCACGGCCAACTGTGTGGTGTTCAGCTGCCCACTCTACAGCTTTGACCGCGCGGCTGTGCTGCATGTCTGGGGCCGTCTCTGGAACAGCACCTTTCTGGAGGAGTACTCAGCTGTGAAGTCCCTGGAAGTGATTGTCCGGGCCAACATCACAGTGAAGTCCTCCATAAAGAACTTGATGCTCCGAGATGCCTCCACAGTGATCCCAGTGATGGTATACTTGGACCCCCTCGAGNOV33c, 253116412SEQ ID NO:818189 aaMW at 20685.5 kDProtein SequenceGSFNLDVMGALRKEGEPGSLFGFSVALHRQLQPRPQSWLLVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQGPGGKIVDCARGTANCVVFSCPLYSFDRAAVLHVWGRLWNSTFLEEYSAVKSLEVIVRANTTVKSSIKNLMLRDASTVIPVMVYLDPLE


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 33B.

TABLE 33BComparison of the NOV33 protein sequences.NOV33aASGICYLFGSLLVELLFSRAVAFNLDVMGALRKEGEPGSLFGFSVALHRQLQPRPQSWLL(SEQ ID NO:814)NOV33b--------------------GSFNLDVMGALRKEGEPGSLFGFSVALHRQLQPRPQSWLL(SEQ ID NO:816)NOV33c--------------------GSFNLDVMGALRKEGEPGSLFGFSVALHRQLQPRPQSWLL(SEQ ID NO:818)NOV33aVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQNOV33bVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQNOV33cVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDQGADMQKESKENQWLGVSVRSQNOV33aGPGGKIVDCARGTANCVVFSCPLYSFDRAAVLHVWGRLWNSTFLEEYSAVKSLEVIVRANNOV33bGPGGKIVDCARGTANCVVFSCPLYSFDRAAVUHVWGRLWNSTFLEEYSAVKSLEVIVRANNOV33cGPGGKIVDCARGTANCVVFSCPLYSFDRAAVLHVWGRLWNSTFLEEYSAVKSLEVIVRANNOV33aITVKSSIKNLMLRDASTVIPVMVYLDPMAVVAEGVPWWVTLLAVLAGLLVLAILVLLLWKNOV33bITVKSSIKNLMLRDASTVIPVMVYLDPMAVVAEGVPWWVTLLAVLAGLLVLALLVLLLWKNOV33cITVKSSIKNLMLRDASTVIPVMVYLDPLE-------------------------------NOV33aCGFFHRSSQSSSFPTNYHRACLAVQPSAMEVGGPGTVG--NOV33bCGFFHRSSQSSSFPTNYHRACLAVQPSAMEVGGPGTVGLENOV33c----------------------------------------


Further analysis of the NOV33a protein yielded the following properties shown in Table 33C.

TABLE 33CProtein Sequence Properties NOV33aSignalP analysis:Cleavage site between residues 23 and 24PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 13; peak value 8.16PSG score:3.76GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.41possible cleavage site: between 22 and 23>>> Seems to have a cleavable signal peptide (1 to 22)ALOM:Klein et al's method for TM region allocationInit position for calculation: 23Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 219-235−15.81PERIPHERALLikelihood = 2.65 (at 136)ALOM score: −15.81 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 11Charge difference: −2.0 C(−1.0)-N( 1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 236 to 278)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):2.41Hyd Moment(95):4.01G content:2D/E content:2S/T content:3Score: −6.94Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 7.9%NLS Score: −0.471KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: 256Dileucine motif in the tail: foundLL at 236checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: vesicles of secretory system11.1%: extracellular, including cell wall>> prediction for CG55908-01 is end (k = 9)


A search of the NOV33a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 33D.

TABLE 33DGeneseq Results for NOV33aNOV33aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG69620Human NOV13 protein - Homo1 . . . 278278/278(100%) e−161sapiens, 278 aa. [WO200250277-A2,1 . . . 278278/278(100%)27-JUN-2002]ABG61788Novel ITGA7 precursor-like receptor58 . . . 278 167/230(72%)3e−84protein #1 - Homo sapiens, 1143 aa.917 . . . 1143 180/230(77%)[WO200229058-A2, 11-APR-2002]ABG61789Novel ITGA7 precursor-like receptor128 . . . 278 150/151(99%)4e−83protein #2 - Homo sapiens, 1035 aa.885 . . . 1035 151/151(99%)[WO200229058-A2, 11-APR-2002]ABU58459Human PRO polypeptide #60 - Homo1 . . . 130129/130(99%)3e−70sapiens, 1141 aa. [US2003027272-12 . . . 141 129/130(99%)A1, 06-FEB-2003]ABU56990Human PRO polypeptide #60 - Homo1 . . . 130129/130(99%)3e−70sapiens, 1141 aa. [US2003027280-12 . . . 141 129/130(99%)A1, 06-FEB-2003]


In a BLAST search of public sequence databases, the NOV33a protein was found to have homology to the proteins shown in the BLASTP data in Table 33E.

TABLE 33EPublic BLASTP Results for NOV33aNOV33aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueCAD48689Sequence 41 from Patent58 . . . 278167/230 (72%)1e−83WO0229058 - Homo sapiens917 . . . 1143180/230 (77%)(Human), 1143 aa.JC5950integrin alpha-7 chain precursor - 1 . . . 130129/130 (99%)9e−70human, 1137 aa.12 . . . 141129/130 (99%)AAH50280Integrin, alpha 7 - Homo sapiens 1 . . . 130129/130 (99%)9e−70(Human), 1137 aa.12 . . . 141129/130 (99%)Q13683Integrin alpha-7 precursor - Homo 1 . . . 130129/130 (99%)9e−70sapiens (Human), 1181 aa.12 . . . 141129/130 (99%)S40148integrin alpha-7A chain - rat, 148150 . . . 278 118/129 (91%)1e−61aa. 1 . . . 129122/129 (94%)


PFam analysis predicts that the NOV33a protein contains the domains shown in the Table 33F.

TABLE 33FDomain Analysis of NOV33aPfamNOV33aIdentities/ExpectDomainMatch RegionSimilarities forValuethe Matched Region


Example 34

The NOV34 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 34A.

TABLE 34ANOV34 Sequence AnalysisNOV34a, CG56077-06SEQ ID NO:8192106 bpDNA SequenceORF Start: at 1ORF Stop: TGA at 1636TCCCGAGACTTTGGAAGTTCTCAGCTATTACTTTATTACATAGGATTTCTGTGTCTTTTCTCATCTCTTTTCCTTTTGGAAAGACCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTGTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTGCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTGTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTGAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCCCACCCACAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCACAGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTGGGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCCGGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTCCTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGCTGCTGTGCCGCGACGGGCCCGGCTTTTACCCGCACCAGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAGGCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCACTGGACCGGTGGCCGCTTCGAGAGACGCACGGACATCCCCGAGGCCGAGGATGTCTATGCCACACGCCACTTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTGATGCGCTGGGACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGCTCATCGCCAGGGACCAGTTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGGTCCTCCGCCTTGAGCCTGACAAGGGGCTCCTGGAGCCACTGCAGGAGCTGGGGCCTCCGGCCCTGGTGGCCCCCCGTGCCTTTGCCCACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAGCATCACGAGATCGACCTCAGTGCCTGAGACCACCAACGGGACTCTGGGCATGGCTGGGGCCCCTGGACGGCCCCTTGGCTGGCTCCTGGCCCTACTTGGGGTGATGGTCCGCCTGTGAGCTGCTGACCGTGGGCCACGTTCATCAGCCACACGTCTAGGCCTTAAGCCCACTTCTTAAAGGATCTGCACCCATGGGGGGACATAGAGGGTCCCAGCCTACTGGACCTCCCGAGCTGCCCTTCAGGTCTAAAGCAACATCTGGACATCTCGGCTGGGGCAGATTCCCCATCAAAGGCAACCTGGGGGGTGCAGGCTGGTGCACTGCATGGAGTCGAGGTGGAGGTCACGTGCAGAGCTCCATGAGGTCCCGGAGCCCCCCTCTCAACCCGCTCCTACCCCGCTGGTCCCTCTGAAAGCACCGAGGGGTTGATGGCATTCCTTTCCCATTCTAAACTCCACGCAGCCAATCGCTTTCCTGGAGGAGCGCCAACCTCGNOV34a, CG56077-06SEQ ID NO:820545 aaMW at 60447.7 kDProtein SequenceSRDFGSSQLLLYYIGFLCLFSSLFLLERPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCPPTELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELPTAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWLEGQPCFVVADASKAGSTTLLCRDGPGFYPHQSLHAWHRDTDAEALELDGRPHLLLASASQRPVLFHWTGGRFERRTDIPEAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLLQQLPSRGAHVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPPALVAPRAFAHITMAGRRFLFAACFKGPTQIYQHHEIDLSANOV34b, CG56077-01SEQ ID NO:8211859 bpDNA SequenceORF Start: ATG at 101ORF Stop: TGA at 1760GTAACTCCTCTTCATCCACTGGGGAGGAAGGTGAGGCAGGCGGGCCCAATTCATTCGCCTCCGGTACTTGCAAGCCTCGCTCAGTCTTAAGCAAGAGGGGATGGATTCGCCCGCAGCACTGAGAATCCAGGGGCAGGCGGGATGGCGTTCAGGCGCTGTTGCTAGAAATCTCTGTCTTTACTCTGTTTTGGTCATTACGGAGGGAAGACAGCCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTCTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTCCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTCTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTAAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCAGAGCCATAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTGGGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCCGGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTCCTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGCTGCTGTGCCGCGACGGGCCCGGCTTTTACCCGCACCAGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAGGCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCACTGGACCGGTGGCCGCTTCGAGAGACGCACAGACATCCCGAGGGCCGAGGATGTCTATGCCACACGCCACTTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTCATGCGCTGGGACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGCTCATCGCCAGGGACCAGCTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGGTCCTCCGCCTTGAGCCTGACAAGGGGCTCCTGGAGCCACTGCAGGAGCTGGGGCCTCCGGCCCTGGTGGCCCCCCGTGCCTTTGCCCACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAGCATCACGAGATCGACCTCAGTGCCTGAGACCACCAACGGGACTCTGGGCATGGCTGGGGCCCCTGGACGGCCCCTTGGCTGGCTCCTGGCCCTACTTGGGGTGATGGCCCGCCTGTGAGCTGCTNOV34b, CG56077-01SEQ ID NO:822553 aaMW at 61118.5 kDProtein SequenceMDSPAALRIQGQAGWRSGAVARNLCLYSVLVITEGRQPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTNVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCRATELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELPAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWLEGQPCFVVADASKAGSTTLLCRDGPGFYPHQSLHAWHRDTDAEALELDGRPHLLLASASQRPVLFHWTGGRFERRTDIPRAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLLQQLPSRGAHVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPPALVAPRAFAHITMAGRRFLFAACFKGPTQIYQHHEIDLSANOV34c, CG56077-02SEQ ID NO:8231482 bpDNA SequenceORF Start: at 1ORF Stop: TGA at 1480TCCCGAGACTTTGGAAGTTCTCAGCTATTACTTTATTACATAGGATTTCTGTGTCTTTTCTCATCTCTTTTCCTTTTGGAAATTGGAAGACCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTGACTGCCCACATCCCCAGCTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTGCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTGTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTGAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCACAGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTGGGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCCGGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTCCTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAGGCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCACTGGACCGGTGGCCGCTTCGAGAGACGCACGGACATCCCCGAGGCCGAGGATGTCTATGCCACACGCCACTTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTCATGCGCTGGGACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGCTCATCGCCAGGGACCAATTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGGTCCTCCGCCTTGAGCCTGACAAGGGGCTCCTGGAGCCACTGCAGGAGCTGGGGCCTCTGGCCCTGGTGGCCCCCCGTGCCTTTGCCCACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAGCATCACGAGATCGACCTCAGTGCCTGANOV34c, CG56077-02SEQ ID NO:824493 aaMW at 54197.8 kDProtein SequenceSRDFGSSQLLLYYIGFLCLFSSLFLLEIGRPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLTAHIPSSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCTAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWLEGQPCFVVADASKAGSTTCSASGPRKSCPSLHAWHRDTDAEALELDGRPHLLLASASQRPVLFHWTGGRFERRTDIPEAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLLQQLPSRGAHVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPLALVAPRAFAHITMAGRRFLFAACFKGPTQIYQHHEIDLSANOV34d, CG56077-03SEQ ID NO:825741 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceACGGAGGGAAGACAGCCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTCTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTCCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTCTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTAAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCACGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCAGAGCCATAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGNOV34d, CG56077-03SEQ ID NO:826247 aaMW at 27380.0 kDProtein SequenceTEGRQPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCRAIELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELNOV34e, CG56077-04SEQ ID NO:827741 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceACGGAGGGAAGACAGCCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTCGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTCCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTCTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTAAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCACGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCAGAGCCATAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGNOV34e, CG56077-04SEQ ID NO:828247 aaMW at 27380.0 kDProtein SequenceTEGRQPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCRAIELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELNOV34f, CG56077-05SEQ ID NO:829741 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceACGGAGGGAAGACAGCCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTCTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTCCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTCTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTAAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCACGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCAGAGCCATAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGNOV34f, CG56077-05SEQ ID NO:830247 aaMW at 27380.0 kDProtein SequenceTEGRQPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCRAIELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELNOV34g, SNP13374715 ofSEQ ID NO:8312106 bpCG56077-06, DNA SequenceORF Start: at 1ORF Stop: TGA at 1636SNP Pos: 1263SNP Change: C to TTCCCGAGACTTTGGAAGTTCTCAGCTATTACTTTATTACATAGGATTTCTGTGTCTTTTCTCATCTCTTTTCCTTTTGGAAAGACCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTGTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTGCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTGTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTGAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCCCACCCACAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCACAGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTGGGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCCGGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTCCTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGCTGCTGTGCCGCGACGGGCCCGGCTTTTACCCGCACCAGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAGGCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCACTGGACCGGTGGCCGCTTCGAGAGACGCACGGACATCCCCGAGGCCGAGGATGTCTATGCCACACGTCACTTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTGATGCGCTGGGACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGCTCATCGCCAGGGACCAGTTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGGTCCTCCGCCTTGAGCCTGACAAGGGGCTCCTGGAGCCACTGCAGGAGCTGGGGCCTCCGGCCCTGGTGGCCCCCCGTGCCTTTGCCCACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAGCATCACGAGATCGACCTCAGTGCCTGAGACCACCAACGGGACTCTGGGCATGGCTGGGGCCCCTGGACGGCCCCTTGGCTGGCTCCTGGCCCTACTTGGGGTGATGGTCCGCCTGTGAGCTGCTGACCGTGGGCCACGTTCATCAGCCACACGTCTAGGCCTTAAGCCCACTTCTTAAAGGATCTGCACCCATGGGGGGACATAGAGGGTCCCAGCCTACTGGACCTCCCGAGCTGCCCTTCAGGTCTAAAGCAACATCTGGACATCTCGGCTGGGGCAGATTCCCCATCAAAGGCAACCTGGGGGGTGCAGGCTGGTGCACTGCATGGAGTCGAGGTGGAGGTCACGTGCAGAGCTCCATGAGGTCCCGGAGCCCCCCTCTCAACCCGCTCCTACCCCGCTGGTCCCTCTGAAAGCACCGAGGGGTTGATGGCATTCCTTTCCCATTCTAAACTCCACGCAGCCAATCGCTTTCCTGGAGGAGCGCCAACCTCGNOV34g, SNP13374715 ofSEQ ID NO:832545 aaMW at 60447.7 kDCG56077-06, Protein SequenceSNP Pos: 421SNP Change: Arg to ArgSRDFGSSQLLLYYIGFLCLFSSLFLLERPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCPPTELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELPTAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWLEGQPCFVVADASKAGSTTLLCRDGPGFYPHQSLHAWHRDTDAEALELDGRPHLLLASASQRPVLFHWTGGRFERRTDIPEAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLLQQLPSRGABVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPPALVAPRAFAHITMAGRRFLFAACFKGPTQIYQHHEIDLSANOV34h, SNP13374714 ofSEQ ID NO:8332106 bpCG56077-06, DNA SequenceORF Start: at 1ORF Stop: TGA at 1636SNP Pos: 1402SNP Change: C to TTCCCGAGACTTTGGAAGTTCTCAGCTATTACTTTATTACATAGGATTTCTGTGTCTTTTCTCATCTCTTTTCCTTTTGGAAAGACCCCCAAAGGGAAAGTGTCCCCTGCGCTGCTCCTGCTCTAAAGACAGCGCCCTGTGTGAGGGCTCCCCGGACCTGCCCGTCAGCTTCTCTCCGACCCTGCTGTCACTGTCACTCGTCAGGACGGGAGTCACCCAGCTGAAGGCCGGCAGCTTCCTGAGAATTCCGTCTCTGCACCTGCTGCTCTTCACCTCCAACTCCTTCTCCGTGATTGAGGACGATGCATTTGCGGGCCTGTCCCACCTGCAGTACCTGTTCATCGAGGACAATGAGATTGGCTCCATCTCTAAGAATGCCCTCAGAGGACTTCGCTCGCTTACACACCTGAGCCTGGCCAATAACCATCTGGAGACCCTCCCCAGATTCCTGTTCCGAGGCCTGGACACCCTTACTCATGTGGACCTCCGCGGGAACCCGTTCCAGTGTGACTGCCGCGTCCTCTGGCTCCTGCAGTGGATGCCCACCGTGAATGCCAGCGTGGGGACCGGCGCCTGTGCGGGCCCCGCCTCCCTGAGCCACATGCAGCTCCACCACCTCGACCCCAAGACTTTCAAGTGCCCACCCACAGAGCTGTCCTGGTTCCAGACGGTGGGGGAGTCGGCACTGAGCGTAGAGCCCTTCTCCTACCAAGGGGAGCCTCACATTGTGCTGGCACAGCCCTTCGCCGGCCGCTGCCTGATTCTCTCCTGGGACTACAGCCTGCAGCGCTTCCGGCCCGAGGAAGAGCTGCCCACAGCGGCCTCCGTGGTGTCCTGCAAGCCACTGGTGCTGGGCCCGAGCCTCTTCGTGCTGGCTGCCCGCCTGTGGGGGGGCTCACAGCTGTGGGCCCGGCCCAGTCCCGGCCTGCGCCTGGCCCCAACGCAGACCCTGGCCCCGCGGCGGCTGCTGCGGCCCAATGACGCCGAGCTCCTGTGGCTGGAAGGGCAACCCTGCTTCGTGGTGGCCGATGCCTCCAAGGCGGGCAGCACCACGCTGCTGTGCCGCGACGGGCCCGGCTTTTACCCGCACCAGAGCCTGCACGCCTGGCACCGGGACACGGACGCTGAGGCCCTGGAGCTGGACGGCCGGCCCCACCTGCTGCTGGCCTCGGCTTCCCAGCGGCCCGTGCTCTTCCACTGGACCGGTGGCCGCTTCGAGAGACGCACGGACATCCCCGAGGCCGAGGATGTCTATGCCACACGCCACTTCCAGGCTGGTGGGGACGTGTTCCTGTGCCTCACACGCTACATTGGGGACTCCATGGTGATGCGCTGGGACGGCTCCATGTTTCGTCTGCTGCAGCAACTTCCCTCGCGCGGTGCCCACGTCTTCCAGCCACTGTTCATCGCCAGGGACCAGTTGGCCATCCTAGGCAGCGACTTCGCCTTCAGCCAGTCCTCCGCCTTGAGCCTGACAAGGGGCTCCTGGAGGCCACTGCAGGAGCTGGGGCCTCCGGCCCTGGTGGCCCCCCGTGCCTTTGCCCACATCACTATGGCCGGCAGACGCTTCCTCTTTGCTGCTTGCTTTAAGGGCCCCACACAGATCTACCAGCATCACGAGATCGACCTCAGTGCCTGAGACCACCACCGGGACTCTGGGCATGGCTGGGGCCCCTGGACGGCCCCTTGGCTGGCTCCTGGCCCTACTTGGGGTGATGGTCCGCCTGTGAGCTGCTGACCGTGGGCCACGTTCATCAGCCACACGTCTAGGCCTTAAGCCCACTTCTTAAAGGATCTGCACCCATGGGGGGACATAGAGGGTCCCAGCCTACTGGACCTCCCGAGCTGCCCTTCAGGTCTAAAGCAACATCTGGACATCTCGGCTGGGGCAGATTCCCCATCAAAGGCAACCTGGGGGGTGCAGGCTGGTGCACTGCATGGAGTCGAGGTGGAGGTCACGTGCAGAGCTCCATGAGGTCCCGGAGCCCCCCTCTCAACCCGCTCCTACCCCGCTGGTCCCTCTGAAAGCACCGAGGGGTTGATGGCATTCCTTTCCCATTCTAAACTCCACGCAGCCAATCGCTTTCCTGGAGGAGCGCCAACCTCGNOV34h, SNP13374714 ofSEQ ID NO:834545 aaMW at 60481.7 kDCG56077-06, Protein SequenceSNP Pos: 468SNP Change: Leu to PheSRDFGSSQLLLYYIGFLCLFSSLFLLERPPKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLSLVRTGVTQLKAGSFLRIPSLHLLLFTSNSFSVIEDDAFAGLSHLQYLFIEDNEIGSISKNALRGLRSLTHLSLANNHLETLPRFLFRGLDTLTHVDLRGNPFQCDCRVLWLLQWMPTVNASVGTGACAGPASLSHMQLHHLDPKTFKCPPTELSWFQTVGESALSVEPFSYQGEPHIVLAQPFAGRCLILSWDYSLQRFRPEEELPTAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWLEGQPCFVVADASKAGSTTLLCRDGPGFYPHQSLHAWHRDTDAEALELDGRPHLLLASASQRPVLFHWTGGRFERRTDIPEAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLLQQLPSRGAHVFQPLFIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPPALVAPRAFAHITMAGRRFLFAACFKGPTQIYQHHEIDLSA


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 34B.

TABLE 34BComparison of the NOV34 protein sequences.NOV34a------------------------------SRDFGSSQLLLYYIGFLCLFSSLFLLERPP(SEQ ID NO:820)NOV34bMDSPAALRIQGQAGWRSGAVARNLCLYSVLVITEG---------------------RQPP(SEQ ID NO:822)NOV34c----------------------------SRDFGSSQLLLYYIGFLCLFSSLFLLEIGRPP(SEQ ID NO:824)NOV34d--------------------------------TEG---------------------RQPP(SEQ ID NO:826)NOV34e--------------------------------TEG---------------------RQPP(SEQ ID NO:828)NOV34f--------------------------------TEG---------------------RQPP(SEQ ID NO:830)NOV34aKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSL------SLVRTGVTQLKAGSFLRIPSNOV34bKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSL------SLVRTGVTQLKAGSFLRIPSNOV34cKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSLTAHIPSSLVRTGVTQLKAGSFLRIPSNOV34dKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSL------SLVRTGVTQLKAGSFLRIPSNOV34eKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSL------SLVRTGVTQLKAGSFLRIPSNOV34fKGKCPLRCSCSKDSALCEGSPDLPVSFSPTLLSL------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-----------------------------------TELSWFQTVGESALNOV34bHLDPKTFKCRA-----------------------------------IELSWFQTVGESALNOV34cHLDPKTFKCTAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLNOV34dHLDPKTFKCRA-----------------------------------IELSWFQTVGESALNOV34eHLDPKTFKCRA-----------------------------------IELSWFQTVGESALNOV34fHLDPKTFKCRA-----------------------------------IELSWFQTVGESALNOV34aS-------------------------------------------------VEPFSYQGEPNOV34bS-------------------------------------------------VEPFSYQGEPNOV34cLRPNDAELLWLEGQPCFVVADASKAGSTTCSASGPRKSCPSLHAWHRDTDAEALELDGRPNOV34dS-------------------------------------------------VEPFSYQGEPNOV34eS-------------------------------------------------VEPFSYQGEPNOV34fS-------------------------------------------------VEPFSYQGEPNOV34aHIVLAQP--------------------------------------------FAGRCLILSNOV34bHIVLAQP--------------------------------------------FAGRCLILSNOV34cHLLLASASQRPVLFHWTGGRFERRTDIPEAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRNOV34dHIVLAQP--------------------------------------------FAGRCLILSNOV34eHIVLAQP--------------------------------------------FAGRCLILSNOV34fHIVLAQP--------------------------------------------FAGRCLILSNOV34aWDYSLQRFR--------------------------------------------PEEELPTNOV34bWDYSLQRFR--------------------------------------------PEEELPANOV34cWDGSMFRLLQQLPSRGAHVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPNOV34dWDYSLQRFR--------------------------------------------PEEEL--NOV34eWDYSLQRFR--------------------------------------------PEEEL--NOV34fWDYSLQRFR--------------------------------------------PEEEL--NOV34aAASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWNOV34bASVVSCKPLVLGPSLFVLAARLWGGSQLWARPSPGLRLAPTQTLAPRRLLRPNDAELLWLNOV34cLALVAPRAFAHITMAGRRFLFAACFKGPTQIYQHHEIDLSA-------------------NOV34d------------------------------------------------------------NOV34e------------------------------------------------------------NOV34f------------------------------------------------------------NOV34aLEGQPCFVVADASKAGSTTLLCRDGPGFYPHQSLHAWHRDTDAEALELDGRPHLLLASASNOV34bEGQPCFVVADASKAGSTTLLCRDGPGFYPHQSLHAWHRDTDAEALELDGRPHLLLASASQNOV34c------------------------------------------------------------NOV34d------------------------------------------------------------NOV34e------------------------------------------------------------NOV34f------------------------------------------------------------NOV34aQRPVLFHWTGGRFERRTDIPEAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLNOV34bRPVLFHWTGGRFERRTDIPRAEDVYATRHFQAGGDVFLCLTRYIGDSMVMRWDGSMFRLLNOV34c------------------------------------------------------------NOV34d------------------------------------------------------------NOV34e------------------------------------------------------------NOV34f------------------------------------------------------------NOV34aLQQLPSRGAHVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPPALVAPRANOV34bQQLPSRGAHVFQPLLIARDQLAILGSDFAFSQVLRLEPDKGLLEPLQELGPPALVAPRAFNOV34c------------------------------------------------------------NOV34d------------------------------------------------------------NOV34e------------------------------------------------------------NOV34f------------------------------------------------------------NOV34aFAHITMAGRPFLFAACFKGPTQIYQHHEIDLSANOV34bAHITMAGRRFLFAACFKGPTQIYQHHEIDLSA-NOV34c---------------------------------NOV34d---------------------------------NOV34e---------------------------------NOV34f---------------------------------


Further analysis of the NOV34a protein yielded the following properties shown in Table 34C.

TABLE 34CProtein Sequence Properties NOV34aSignalP analysis:Cleavage site between residues 26 and 27PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 3; pos. chg 1; neg. chg 1H-region:length 23; peak value 10.35PSG score:5.95GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −5.07possible cleavage site: between 25 and 26>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 9-25−5.57PERIPHERALLikelihood = 2.70 (at 82)ALOM score: −5.57 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 16Charge difference: 2.0 C(3.0)-N(1.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptide>>> membrane topology: type 1b (cytoplasmic tail 9 to 545)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):11.37Hyd Moment(95):7.33G content: 2D/E content:2S/T content: 5Score: −4.71Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 47 LRC|SCNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.8%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: RDFGnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 9LL at 10LL at 25LL at 61LL at 87LL at 88LL at 176LL at 322LL at 330LL at 352LL at 386LL at 387checking 63 PROSITE DNA binding motifs:Leucine zipper pattern (PS00029): *** found *** LSLANNHLETLPRFLFRGLDTL at 136nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: nuclear21.7%: mitochondrial21.7%: cytoplasmic 8.7%: vesicles of secretory system 4.3%: vacuolar 4.3%: endoplasmic reticulum 4.3%: peroxisomal>> prediction for CG56077-06 is nuc (k = 23)


A search of the NOV34a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 34D.

TABLE 34DGeneseq Results for NOV34aNOV34aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABB09515Leu-rich glioma-inactivated protein-like17 . . . 545 518/531 (97%)0.0NOV7a protein, SEQ ID NO: 28 - Homo24 . . . 553 521/531 (97%)sapiens, 553 aa. [WO200253742-A2,11-JUL-2002]ABB98246Human CGDD SEQ ID NO 4 - Homo5 . . . 545519/541 (95%)0.0sapiens, 537 aa. [WO200248368-A2,3 . . . 537523/541 (95%)20-JUN-2002]AAE25347Human LP polypeptide, LP188 - Homo5 . . . 545519/541 (95%)0.0sapiens, 537 aa. [WO200248361-A2,3 . . . 537523/541 (95%)20-JUN-2002]ABG91403Primate LP286 - Mammalia, 513 aa.5 . . . 545495/541 (91%)0.0[WO200263009-A2, 15-AUG-2002]3 . . . 513499/541 (91%)ABU11596Human MDDT polypeptide SEQ ID 543 -55 . . . 545 482/493 (97%)0.0Homo sapiens, 567 aa.76 . . . 567 483/493 (97%)[WO200279449-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV34a protein was found to have homology to the proteins shown in the BLASTP data in Table 34E.

TABLE 34EPublic BLASTP Results for NOV34aNOV34aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8N135LGI4 (Leucine-rich glioma inactivated5 . . . 545519/541 (95%)0.0protein 4) (LGI1-like protein 3) - Homo  3 . . . 537 523/541 (95%)sapiens (Human), 537 aa.Q8K1S1LGI1-like protein 3 - Mus musculus16 . . . 545 460/530 (86%)0.0(Mouse), 537 aa.9 . . . 537491/530 (91%)Q8K4Y8Leucine-rich glioma inactivated16 . . . 545 458/530 (86%)0.0protein 4 - Mus musculus (Mouse),9 . . . 537489/530 (91%)537 aa.Q9JIA1Leucine-rich glioma-inactivated 11 . . . 545275/556 (49%)e−152protein - Mus musculus (Mouse), 557 aa.6 . . . 557375/556 (66%)Q8K4Y5Leucine-rich glioma inactivated16 . . . 545 270/540 (50%)e−151protein 1 - Rattus norvegicus (Rat),22 . . . 557 367/540 (67%)557 aa.


PFam analysis predicts that the NOV34a protein contains the domains shown in the Table 34F.

TABLE 34FDomain Analysis of NOV34aIdentities/Similarities forPfamNOV34athe MatchedExpectDomainMatch RegionRegionValueLRR108 . . . 1317/25(28%)0.09921/25(84%)LRR132 . . . 15510/25(40%)0.05820/25(80%)LRRCT165 . . . 21421/54(39%)  1.1e−1036/54(67%)EPTP222 . . . 35430/147(20%)  1.7e−0773/147(50%)EPTP405 . . . 53758/144(40%)   8e−3990/144(62%)


Example 35

The NOV35 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 35A.

TABLE 35ANOV35 Sequence AnalysisNOV35a, CG56110-03SEQ ID NO:835874 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 871ATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGATACACATTTGGAGGAGACGTAAANOV35a, CG56110-03SEQ ID NO:835290 aaMW at 33275.0 kDProtein SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQHARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35b, CG56110-07SEQ ID NO:837720 bpDNA SequenceORF Start: ATG at 3ORF Stop: TAG at 510TCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGGNOV35b, CG56110-07SEQ ID NO:838169 aaMW at 19327.9 kDProtein SequenceMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKARVIWTSSDHQVLSGDNOV35c, 274082305SEQ ID NO:839900 bpDNA SequenceORF Start: at 2ORF Stop: TAA at 887CACCAGATCTCCCACCATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGATACACATTTGGAGGAGACGTAAAGTCGACGGCCNOV35c, 274082305SEQ ID NO:840295 aaMW at 33817.6 kDProtein SequenceTRSPTMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35d, CG56110-01SEQ ID NO:841873 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 871ATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGATACACATTTGGAGGAGACGTAANOV35d, CG56110-01SEQ ID NO:842290 aaMW at 33275.0 kDProtein SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRAELLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRNMDVKKCGIQDTNSKKQSDTHLEETNOV35e, CG56110-02SEQ ID NO:843666 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACNOV35e, CG56110-02SEQ ID NO:844222 aaMW at 25431.6 kDProtein SequenceFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHNOV35f, CG56110-04SEQ ID NO:845745 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 535ATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGGNOV35f, CG56110-04SEQ ID NO:846178 aaMW at 20453.3 kDProtein SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGDNOV35g, CG56110-05SEQ ID NO:8477184 bpDNA SequenceORF Start: ATG at 62ORF Stop: TAA at 932GCAGCTTCCCGAGGCTCCGCACCAGCCGCGCTTCTGTCCGCCTGCAGGGCATTCCAGAAAGATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGATACACATTTGGAGGAGACGTAATCCAGCATTGGAACTTCTGATCTTCAAGCAGGGATTCTCAACCTGTGGTTTAGGGGTTCATCGGGGCTGAGCGTGACAAGAGGAAGGAATGGGCCCGTGGGATGCAGGCAATGTGGGACTTAAAAGGCCCAAGCACTGAAAATGGAACCTGGCGAAAGCAGAGGAGGAGAATGAAGAAAGATGGAGTCAAACAGGGAGCCTGGAGGGAGACCTTGATACTTTCAAATGCCTGAGGGGCTCATCGACGCCTGTGACAGGGAGAAAGGATACTTCTGAACAAGGAGCCTCCAAGCAAATCATCCATTGCTCATCCTAGGAAGACGGGTTGAGAATCCCTAATTTGAGGGTCAGTTCCTGCAGAAGTGCCCTTTGCCTCCACTCAATGCCTCAATTTGTTTTCTGCATGACTGAGAGTCTCAGTGTTGGAACGGGACAGTATTTATGTATGAGTTTTTCCTATTTATTTTGAGTCTGTGAGGTCTTCTTGTCATGTGAGTGTGGTTGTGAATGATTTCTTTTGAAGATATATTGTAGTAGATGTTACAATTTTGTCGCCAAACTAAACTTGCTGCTTAATGATTTGCTCACATCTAGTAAAACATGGAGTATTTGTAAGGTGCTTGGTCTCCTCTATAACTACAAGTATACATTGGAAGCATAAAGATCAAACCGTTGGTTGCATAGGATGTCACCTTTATTTAACCCATTAATACTCTGGTTGACCTAATCTTATTCTCAGACCTCAAGTGTCTGTGCAGTATCTGTTCCATTTAAATATCAGCTTTACAATTATGTGGTAGCCTACACACATAATCTCATTTCATCGCTGTAACCACCCTGTTGTGATAACCACTATTATTTTACCCATCGTACAGCTGAGGAAGCAAACAGATTAAGTAACTTGCCCAAACCAGTAAATAGCAGACCTCAGACTGCCACCCACTGTCCTTTTATAATACAATTTACAGCTATATTTTACTTTAAGCAATTCTTTTATTCAAAAACCATTTATTAAGTGCCCTTGCAATATCAATCGCTGTGCCAGGCATTGAATCTACAGATGTGAGCAAGACAAAGTACCTGTCCTCAAGGAGCTCATAGTATAATGAGGAGATTAACAAGAAAATGTATTATTACAATTTAGTCCAGTGTCATAGCATAAGGATGATGCGAGGGGAAAACCCGAGCAGTGTTGCCAAGAGGAGGAAATAGGCCAATGTGGTCTGGGACGGTTGGATATACTTAAACATCTTAATAATCAGAGTAATTTTCATTTACAAAGAGAGGTCGGTACTTAAAATAACCCTGAAAAATAACACTGGAATTCCTTTTCTAGCATTATATTTATTCCTGATTTGCCTTTGCCATATAATCTAATGCTTGTTTATATAGTGTCTGGTATTGTTTAACAGTTCTGTCTTTTCTATTTAAATGCCACTAAATTTTAAATTCATACCTTTCCATGATTCAAAATTCAAAAGATCCCATGGGAGATGGTTGGAAAATCTCCACTTCATCCTCCAAGCCATTCAAGTTTCCTTTCCAGAAGCAACTGCTACTGCCTTTCATTCATATGTTCTTCTAAAGATAGTCTACATTTGGAAATGTATGTTAAAAGCACGTATTTTTAAAATTTTTTTCCTAAATAGTAACACATTGTATGTCTGCTGTGTACTTTGCTATTTTTATTTATTTTAGTGTTTCTTATATAGCAGATGGAATGAATTTGAAGTTCCCAGGGCTGAGGATCCATGCCTTCTTTGTTTCTAAGTTATCTTTCCCATACCTTTTCATTATCTTTCATATGATCCAGTATATGTTAAATATGTCCTACATATACATTTAGACAACCACCATTTGTTAAGTATTTGCTCTAGGACAGAGTTTGGATTTGTTTATGTTTGCTCAAAAGGAGACCCATGGGCTCTCCAGGGTGCACTGAGTCAATCTAGTCCTAAAAAGCAATCTTATTATTAACTCTGTATGACAGAATCATGTCTGGAACTTTTGTTTTCTGCTTTCTGTCAAGTATAAACTTCACTTTGATGCTGTACTTGCAAAATCACATTTTCTTTCTGGAAATTCCGGCAGTGTACCTTGACTGCTAGCTACCCTGTGCCAGAAAAGCCTCATTCGTTGTGCTTGAACCCTTGAATGCCACCAGCTGTCATCACTACACAGCCCTCCTAAGAGCCTTCCTGGAGGTTTCGAGATTCAGATGCCCTGGGAGATCCCAGAGTTTCCTTTCCCTCTTGGCCATATTCTGGTGTCAATGACAAGGAGTACCTTGGCTTTGCCACATGTCAAGGCTGAAGAAACAGTGTCTCCAACAGAGCTCCTTGTGTTATCTGTTTGTACATGTGCATTTGTACAGTAATTGGTGTGACAGTGTTCTTTGTGTGAATTACAGGCAAGAATTGTGGCTGAGCAAGGCACATAGTCTACTCAGTCTATTCCTAAGTCCTAACTCCTCCTTGTGGTGTTGGATTTGTAAGGCACTTTATCCCTTTTGTCTCATGTTTCATCGTAAATGGCATAGGCAGAGATGATACCTAATTCTGCATTTGATTGTCACTTTTTGTACCTGCATTAATTTAATAAAATATTCTTATTTATTTTGTTACTTGGTAAAAAAAAAAAAAAAGCTTCCCGAGGCTCCGCACCAGCCGCGCTTCTGTCCGCCTGCAGGGCATTCCAGAAAGATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGATACACATTTGGAGGAGACGTAATCCAGCATTGGAACTTCTGATCTTCAAGCAGGGATTCTCAACCTGTGGTTTAGGGGTTCATCGGGGCTGAGCGTGACAAGAGGAAGGAATGGACCCGTGGGATGCAGGCAATGTGGGACTTAAAAGGCCCAAGCACTGAAAATGGAACCTGGCGAAAGCAGAGGAGGAGAATGAAGAAAGATGGAGTCAAACAGGGAGCCTGGAGGGAGACCTTGATACTTTCAAATGCCTGAGGGGCTCATCGACGCCTGTGACAGGGAGAAAGGATACTTCTGAACAAGGAGCCTCCAAGCAAATCATCCATTGCTCATCCTAGGAAGACGGGTTGAGAATCCCTAATTTGAGGGTCAGTTCCTGCAGAAGTGCCCTTTGCCTCCACTCAATGCCTCAATTTGTTTTCTGCATGACTGAGAGTCTCAGTGTTGGAACGGGACAGTATTTATGTATGAGTTTTTCCTATTTATTTTGAGTCTGTGAGGTCTTCTTGTCATGTGAGTGTGGTTGTGAATGATTTCTTTTGAACATATATTGTAGTAGATGTTACAATTTTGTCGCCAAACTAAACTTGCTGCTTAATGATTTGCTCACATCTAGTAAAACATGGAGTATTTGTAAGGTGCTTGGTCTCCTCTATAACTACAAGTATACATTGGAAGCATAAAGATCAAACCGTTGGTTGCATAGGATGTCACCTTTATTTAACCCATTAATACTCTGGTTGACCTAATCTTATTCTCAGACCTCAAGTGTCTGTGCAGTATCTGTTCCATTTAAATATCAGCTTTACAATTATGTGGTAGCCTACACACATAATCTCATTTCATCGCTGTAACCACCCTGTTGTGATAACCACTATTATTTTACCCATCGTACAGCTGAGGAAGCAAACAGATTAAGTAACTTGCCCAAACCAGTAAATAGCAGACCTCAGACTGCCACCCACTGTCCTTTTATAATACAATTTACAGCTATATTTTACTTTAAGCAATTCTTTTATTCAAAAACCATTTATTAAGTGCCCTTGCAATATCAATCGCTGTGCCAGGCATTGAATCTACAGATGTGAGCAAGACAAAGTACCTGTCCTCAAGGAGCTCATAGTATAATCAGGAGATTAACAAGAAAATGTATTATTACAATTTAGTCCAGTGTCATAGCATAAGGATGATGCGAGGGGAAAACCCGAGCAGTGTTGCCAAGAGCAGGAAATAGGCCAATGTGGTCTGGGACGGTTGGATATACTTAAAGATCTTAATAATCAGAGTAATTTTCATTTACAAAGAGAGGTCGGTACTTAAAATAACCCTGAAAAATAACACTGGAATTCCTTTTCTAGCATTATATTTATTCCTGATTTGCCTTTGCCATATAATCTAATGCTTGTTTATATAGTGTCTGGTATTGTTTAACAGTTCTGTCTTTTCTATTTAAATGCCACTAAATTTTAAATTCATACCTTTCCATGATTCAAAATTCAAAAGATCCCATGGGAGATGGTTGGAAAATCTCCACTTCATCCTCCAAGCCATTCAAGTTTCCTTTCCAGAAGCAACTGCTACTGCCTTTCATTCATATGTTCTTCTAAAGATAGTCTACATTTGGAAATGTATGTTAAAAGCACGTATTTTTAAAATTTTTTTCCTAAATAGTAACACATTGTATGTCTGCTGTGTACTTTCCTATTTTTATTTATTTTAGTGTTTCTTATATAGCAGATGGAATGAATTTGAAGTTCCCAGGGCTGAGGATCCATGCCTTCTTTGTTTCTAAGTTATCTTTCCCATAGCTTTTCATTATCTTTCATATGATCCAGTATATGTTAAATATGTCCTACATATACATTTAGACAACCACCATTTGTTAAGTATTTGCTCTAGGACAGAGTTTGGATTTGTTTATGTTTGCTCAAAAGGAGACCCATGGGCTCTCCAGGGTGCACTGAGTCAATCTAGTCCTAAAAAGCAATCTTATTATTAACTCTGTATGACAGAATCATGTCTGGAACTTTTGTTTTCTGCTTTCTGTCAAGTATAAACTTCACTTTGATGCTGTACTTGCAAAATCACATTTTCTTTCTGGAAATTCCGGCAGTGTACCTTGACTGCTAGCTACCCTGTGCCAGAAAAGCCTCATTCGTTGTGCTTGAACCCTTGAATGCCACCAGCTGTCATCACTACACAGCCCTCCTAAGAGGCTTCCTGGAGGTTTCGAGATTCAGATGCCCTGGGAGATCCCAGAGTTTCCTTTCCCTCTTGGCCATATTCTGGTGTCAATGACAAGGAGTACCTTGGCTTTGCCACATGTCAAGGCTGAAGAAACAGTGTCTCCAACAGAGCTCCTTGTGTTATCTGTTTGTACATGTGCATTTGTACAGTAATTGGTGTGACAGTGTTCTTTGTGTGAATTACAGGCAAGAATTGTGGCTGAGCAAGGCACATAGTCTACTCAGTCTATTCCTAAGTCCTAACTCCTCCTTGTGGTGTTGGATTTGTAAGGCACTTTATCCCTTTTGTCTCATGTTTCATCGTAAATGGCATAGGCAGAGATGATACCTAATTCTGCATTTGATTGTCACTTTTTGTACCTGCATTAATTTAATAAAATATTCTTATTTATTTTGTTACTTGGTACACCAGCATGTCCATTTTCTTGTTTATTTTGTGTTTAATAAAATGNOV35g, CG56110-05SEQ ID NO:848290 aaMW at 33275.0 kDProtein SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35h, CG56110-06SEQ ID NO:849900 bpDNA SequenceORF Start: ATG at 17ORF Stop: at 866CACCAGATCTCCCACCATGAGGATATTTGCTGTCTTTATATTCATGACCTACTGGCATTTGCTGAACGCATTTACTGTCACGGTTCCCAAGGACCTATATGTGGTAGAGTATGGTAGCAATATGACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCACTAATTGTCTATTGGGAAATGGAGGATAAGAACATTATTCAATTTGTGCATGGAGAGGAAGACCTGAAGGTTCAGCATAGTAGCTACAGACAGAGGGCCCGGCTGTTGAAGGACCAGCTCTCCCTGGGAAATGCTGCACTTCAGATCACAGATGTGAAATTGCAGGATGCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCGAATTACTGTGAAAGTCAATGCCCCATACAACAAAATCAACCAAAGAATTTTGGTTGTGGATCCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAAGGCCGAAGTCATCTGGACAAGCAGTGACCATCAAGTCCTGAGTGGTAAGACCACCACCACCAATTCCAAGAGAGAGGAGAAGCTTTTCAATGTGACCAGCACACTGAGAATCAACACAACAACTAATGAGATTTTCTACTGCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTCATCCCAGAACTACCTCTGGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCTTATTATGCCTTGGTGTAGCACTGACATTCATCTTCCGTTTAAGAAAAGGGAGAATGATGGATGTGAAAAAATGTGGCATCCAAGATACAAACTCAAAGAAGCAAAGTGATACACATTTGGAGGAGACGTAAAGTCGACGGCCNOV35h, CG56110-06SEQ ID NO:850283 aaMW at 32449.2 kDProtein SequenceMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQS


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 35B.

TABLE 35BComparison of the NOV35 protein sequences.NOV35a-----MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:836)NOV35b--------------MTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:838)NOV35cTRSPTMRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:840)NOV35d-----MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:842)NOV35e-----------------------FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:844)NOV35f-----MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:846)NOV35g-----MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:848)NOV35h-----MRIFAVFIFMTYWHLLNAFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIV(SEQ ID NO:850)NOV35aYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35bYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35cYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35dYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35eYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35fYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35gYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35hYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMNOV35aISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35bISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35cISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35dISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35eISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35fISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35gISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35hISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLNOV35aSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNOV35bSGD---------------------------------------------------------NOV35cSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNOV35dSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNOV35eSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNOV35fSGD---------------------------------------------------------NOV35gSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNOV35hSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNOV35aNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35b-------------------------------------------------------NOV35cNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35dNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35eNERTH--------------------------------------------------NOV35f-------------------------------------------------------NOV35gNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEETNOV35hNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQS-------


Further analysis of the NOV35a protein yielded the following properties shown in Table 35C.

TABLE 35CProtein Sequence Properties NOV35aSignalP analysis:Cleavage site between residues 19 and 20PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 2; pos. chg 1; neg. chg 0H-region:length 22; peak value 13.04PSG score:8.64GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −3.72possible cleavage site: between 18 and 19>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 242-258−11.36PERIPHERALLikelihood = 7.90 (at 40)ALOM score: −11.36 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 249Charge difference: 5.0 C(5.0)-N(0.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptide>>> Single TMS is located near the C-terminus>>> membrane topology: type Nt (cytoplasmic tail 1 to 241)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):5.40Hyd Moment(95):9.37G content:0D/E content:1S/T content:3Score: −2.89Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 12 MRI|FANUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 11.7%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: RIFAnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 15LL at 87checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):30.4%: nuclear26.1%: cytoplasmic13.0%: Golgi13.0%: endoplasmic reticulum 8.7%: mitochondrial 4.3%: vesicles of secretory system 4.3%: peroxisomal>> prediction for CG56110-03 is nuc (k = 23)


A search of the NOV35a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 35D.

TABLE 35DGeneseq Results for NOV35aNOV35aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #,Date]ResiduesRegionValueAAE33390Human membrane B7-4 (B7-4M)1 . . . 290290/290 (100%)e−169protein - Homo sapiens, 290 aa.1 . . . 290290/290 (100%)[WO200279499-A1, 10-OCT-2002]ABJ18518Human PD-L1M protein - Homo1 . . . 290290/290 (100%)e−169sapiens, 290 aa. [WO200278731-A1,1 . . . 290290/290 (100%)10-OCT-2002]AAE16964Human PD-1 ligand (PD-L1) - Homo1 . . . 290290/290 (100%)e−169sapiens, 290 aa. [WO200200730-A2,1 . . . 290290/290 (100%)03-JAN-2002]AAU81007B7-related protein, BSL1 - Homo1 . . . 290290/290 (100%)e−169sapiens, 290 aa. [WO200194413-A2,1 . . . 290290/290 (100%)13-DEC-2001]ABG64016Human albumin fusion protein #691 -1 . . . 290290/290 (100%)e−169Homo sapiens, 290 aa.1 . . . 290290/290 (100%)[WO200177137-A1, 18-OCT-2001]


In a BLAST search of public sequence databases, the NOV35a protein was found to have homology to the proteins shown in the BLASTP data in Table 35E.

TABLE 35EPublic BLASTP Results for NOV35aNOV35aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9NZQ7B7-H1 (PD-1-ligand precursor) -1 . . . 290290/290 (100%)e−168Homo sapiens (Human), 290 aa.1 . . . 290290/290 (100%)CAC34696Sequence 1 from Patent1 . . . 227227/227 (100%)e−130WO0114557 - Homo sapiens1 . . . 227227/227 (100%)(Human), 245 aa.Q9EP73PD-1-ligand precursor (B7-H11 . . . 290202/291 (69%) e−114protein) - Mus musculus (Mouse),1 . . . 290236/291 (80%) 290 aa.Q9NUZ5Hypothetical protein FLJ11032 -115 . . . 290 176/176 (100%)7e−99 Homo sapiens (Human), 176 aa.1 . . . 176176/176 (100%)Q9BQ51Butyrophilin precursor B7-DC (PD-19 . . . 240 90/223 (40%)2e−35 1-ligand 2 protein) - Homo sapiens21 . . . 218 123/223 (54%) (Human), 273 aa.


PFam analysis predicts that the NOV35a protein contains the domains shown in the Table 35F.

TABLE 35FDomain Analysis of NOV35aIdentities/PfamNOV35aSimilarities forExpectDomainMatch Regionthe Matched RegionValueig33 . . . 11613/85 (15%)0.1354/85 (64%)


Example 36

The NOV36 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 36A.

TABLE 36ANOV36 Sequence AnalysisNOV36a, CG56383-02SEQ ID NO:8512715 bpDNA SequenceORF Start: ATG at 34ORF Stop: TAA at 2566TGCCTGCACTGCAAGTGTCCCCAGGAGGAGCACATGGTGACAGTGATGCCGCTGGAGATGGAGAAGACCATCAGCAAACTCATGTTTGACTTTCAGAGGAACTCGACCTCAGATGATGACTCAGGCTGTGCTTTGGAAGAGTATGCCTGGGTCCCGCCGGGTCTGAAGCCTGAACAGGTACACCAGTACTATAGCTGTCTCCCAGAAGAGAAAGTCCCTTATGTCAACAGTCCTGGAGAGAAACTGCGAATCAAGCAGCTACTACACCAGCTGCCGCCACATGACAATGAGGTAAGGTATTGCAACTCCCTGGATGAGGAAGAGAAGAGGGAGCTGAAGCTTTTCAGCAGCCAGAGGAAACGCGAAAACTTGGGCCGCGGGAATGTCAGGCCTTTCCCAGTCACCATGACAGGAGCTATTTGTGAACAGTGCGGAGGCCAGATCAATGGTGGAGACATCGCTGTGTTTGCGTCACGCGCTGGCCACGGCGTTTGCTGGCACCCGCCGTGCTTCGTATGCACTGTCTGCAATGAGCTCCTGGTGGATCTGATCTACTTTTACCAAGATGGGAAGATATACTGTGGCAGGCACCATGCTGAGTGCCTGAAGCCGCGCTGTGCTGCCTGCGATGAGATCATCTTTGCAGATGAATGCACAGAAGCTGAGGGGCGACACTGGCACATGAAACACTTTTGCTGCTTCGAGTGTGAGACAGTGCTGGGCGGCCAGCGCTACATCATGAAGGAGGGAAGACCCTACTGTTGCCACTGCTTCGAGTCCTTGTATGCAGAATATTGTGACACCTGTGCCCAACATATAGGTATCGACCAAGGTCAAATGACCTATGATGGCCAACACTGGCATGCCACTGAGACCTGTTTCTGCTGTGCTCACTGCAAGAAATCCCTCCTGGGGCGGCCATTCCTCCCGAAGCAGGGCCAGATATTCTGCTCACGGGCCTGCAGTGCTGGGGAAGACCCCAATGGTTCTGACTCCTCTGATTCCGCCTTCCAGAACGCCAGGGCCAAGGAGTCCCGGCGCAGTGCCAAAATTGGCAAGAACAAGGGCAAGACGGAGGAGCCCATGCTGAACCAGCACAGCCAGCTGCAAGTGAGTTCTAACCGGCTGTCAGCCGACGTAGACCCCCTGTCACTGCAGATGGACATGCTCAGCCTGTCCAGCCAGACACCCAGCCTCAACCGGGACCCCATCTGGAGGAGCCGGGAAGAGCCCTACCATTATGGGAACAAGATGGAGCAGAACCAGACCCAGAGCCCTCTGCAGCTCCTCAGCCAGTGCAACATCAGAACTTCCTACAGTCCAGGAGGGCAAGGGGCTGGGGCCCAGCCCGAAATGTGGGGCAAGCACTTCAGCAACCCCAAAAGGAGCTCGTCACTGGCCATGACAGGACATGCTGGCAGCTTCATCAAGGAATGCCGAGAAGACTATTACCCGGGGAGGCTGAGATCTCAGGAGAGCTACAGTGATATGTCTAGTCAGAGTTTCAGTGAGACCCGAGGCAGCATCCAAGTCCCCAAATATGAGGAGGAAGAGGAAGAGGAAGGGGGCTTGTCCACTCAGCAGTGTCGGACCCGTCATCCCATCAGTTCCCTGAAATACACAGAGGACATGACGCCCACAGAGCAGACCCCTCGGGGCTCCATGGAATCCCTGGCCCTGTCTAATGCAACAGGTCTCTCTGCTGATGGTGGTGCCAAGCGCCAGGAGCACCTATCCCGATTTTCCATGCCTGACCTCAGCAAAGACTCTGGAATGAATGTGTCTGAGAAGCTGAGCAACATGGGCACTCTTAACTCGTCCATGCAGTTCCGGAGCGCAGAGTCAGTTCGCAGCCTGCTCTCTGCCCAGCAGTACCAGGAGATGGAGGGAAACCTCCACCAGCTCAGCAACCCCATTGGCTACAGAGACCTGCAGTCCCACGGAAGGATGCATCAGAGCTTTGATTTTGATGGAGGGATGGCGGGCAGCAAGCTGCCAGGGCAGGAGGGCGTGAGGATCCAGCCCATGAGTGAACGCACCCGGAGAAGAGCTACTTCACGCGACGACAACCGCCGTTTCCGACCTCACAGGTCCAGGCGTTCCCGACGCTCTCGCTCCGACAACGCCCTCCACCTGGCCAGCGAACGCGAGGCCATCTCCCGGTTAAAAGATAGGCCCCCTCTGAGAGCCAGGGAGGACTATGACCAATTTATGCGCCAGCGGAGCTTCCAGGAGAGCATGGGGCATGGGTCCCGGAGGGACCTGTACGGCCAGTGCCCTAGGACTGTGTCGGACCTGGCTTTGCAGAATGCCTTTGGGGACCGCTGGGGACCCTACTTCGCCGAGTATGATTGGTGTTCCACCTGCTCCTCCTCTTCAGAGTCTGACAACGAGGGCTATTTCCTAGGAGAACCCATCCCCCAGCCAGCGCGCCTGCGATACGTCACAAGCGATGAGCTGCTGCACAAATACAGCTCCTACGGCCTCCCCAAATCTTCCACATTAGGTGGCAGAGGACAGTTGCACAGCAGGAAAAGACAGAAGAGCAAAAACTGTATCATTTCTTAATATGATTGGGATCAGGGAATGGGAGAAGATGGGAGCTAAGAATGTAAAGTCAGAAACTTGCACTGTTTTAAATGTTAAAGCGCTTTTGGGGGTGGCTTATGGGGGAGAAAAGGGAAAATGCTGTCAGTAGATGGAGGCAAGGTTACANOV36a, CG56383-02SEQ ID NO:852844 aaMW at 95613.8 kDProtein SequenceMVTVMPLEMEKTISKLMFDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSCLPEEKVPYVNSPGEKLRIKQLLHQLPPHDNEVRYCNSLDEEEKRELKLFSSQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPPCFVCTVCNELLVDLIYFYQDGKIYCGRHHAECLKPRCAACDEIIFADECTEAEGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCHCFESLYAEYCDTCAQHIGIDQGQMTYDGQHWHATETCFCCAHCKKSLLGRPFLPKQGQIFCSRACSAGEDPNGSDSSDSAFQNARAKESRRSAKIGKNKGKTEEPMLNQHSQLQVSSNRLSADVDPLSLQMDMLSLSSQTPSLNRDPIWRSREEPYHYGNKMEQNQTQSPLQLLSQCNIRTSYSPGGQGAGAQPEMWGKHFSNPKRSSSLAMTGHAGSFIKECREDYYPGRLRSQESYSDMSSQSFSETRGSIQVPKYEEEEEEEGGLSTQQCRTRHPISSLKYTEDMTPTEQTPRGSMESLALSNATGLSADGGAKRQEHLSRFSMPDLSKDSGMINSEKLSNMGTLNSSMQFRSAESVRSLLSAQQYQEMEGNLHQLSNPIGURDLQSHGRMHQSFDFDGGMAGSKLPGQEGVRIQPMSERTRRRATSRDDNRRFRPHRSRRSRRSRSDNALHLASEREAISRLKDRPPLRAREDYDQFMRQRSFQESMGHGSRRDLYGQCPRTVSDLALQNAFGDRWGPYFAEYDWCSTCSSSSESDNEGYFLGEPIPQPARLRYVTSDELLHKYSSYGLPKSSTLGGRGQLHSRKRQKSKNCIISNOV36b, CG56383-01SEQ ID NO:8531190 bpDNA SequenceORF Start: ATG at 54ORF Stop: TGA at 1014TTATTGACAGTTTATCCTGCCGCACCTGGAATCCTGAGACAAACCAAGGTGCTATGTGTTTCACGTCCCAGTGCAGAGCTCTGAGCAGCTCATCAGCCTCTCCAATGTCTCTCATTTTTTTAGGTATCGACCAAGGTCAAATGACCTATGACGGCCAACACTGGCATGCCACTGAGACCTGTTTCTGCTGTGCTCACTGCAAGAAATCCCTCCTGGGGCGGCCATTCCTCCCGAAGCAGGGCCAGATATTCTGCTCACGGGCCTGCAGTGCTGGGGAAGACCCCAATGGTTCTGACTCCTCTGATTCCGCCTTCCAGAACGCCAGGGCCAAGGAGTCCCGGCGCAGTGCCAAAATTGGCAAGAACAAGGGCAAGACGGAGGAGCCCATGCTGAACCAGCACAGCCAGCTGCAAGTGAGTTCTAACCGGCTGTCAGCCGACGTAGACCCCCTGTCACTGCAGATGGACATGCTCAGCCTGTCCAGCCAGACACCCAGCCTCAACCGGGACCCCATCTGGAGGAGCCGGGAAGAGCCCTACCATTAAGGGAACAAGATGGAGCAGAACCAGACCCAGAGCCCTCTGCAGCTCCTCAGCCAGTGCAACATCAGAACTTCCTACAGTCCAGGAGGGCAAGGGGCTGGGGCCCAGCCCGAAATGTGGGGCAAGCACTTCAGCAACCCCAAAAGGAGCACGTCACTGGCCATGACAGGACATGCTGGCAGCTTCATCAAGGAATGCCGAGAAGACTATTACCCGGGGAGGCTGAGATCTCAGGAGAGCTACAGTGATATGTCTAGTCAGAGTTTCAGTGAGACCCGAGGCAGCATCCAAGTCCCCAAATATGAGGAGGAAGAGGAAGAGGAAGGGGGCTTGTCCACTCAGCAGTGTCGGACCCGTCATCCCATCAGTTCCCTGAAATACACAGAGGACATGACGCCCACAGAGCAGACCCCTCGGGGCTCCATGGAATCCCTGGCCCTGTCTAATGCAACAGGTAGGTTCTGTTCACCTTGAAAACAGATAGAAGGGGGTAGTCTCTGGGTGACTGGATGCTGGTCCCCAGGAATTTTTTTTTTTTTGAAATGGAGTCTCGCTCTGTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCCGCTCACTGCAAGCTCCACCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCACGANOV36b, CG56383-01SEQ ID NO:854320 aaMW at 35524.0 kDProtein SequenceMCFTSQCRALSSSSASPMSLTFLGIDQGQMTYDGQHWHATETCFCCAHCKKSLLGRPFLPKQGQIFCSEACSAGEDPNGSDSSDSAFQNARAXESRRSAKIGKNKGKTEEPMLNQHSQLQVSSNRLSADVDPLSLQMDMLSLSSQTPSLNRDPIWRSREEPYHYGNKMEQNQTQSPLQLLSQCNIRTSYSPGGQGAGAQPEMWGKHFSNPKRSTSLAMTGHAGSFIKECREDYYPGRLRSQESYSDMSSQSFSETRGSIQVPKYEEEEEEEGGLSTQQCRTRHPISSLKYTEDMTPTEQTPRGSMESLALSNATGRFCSPNOV36c, SNP13382513 ofSEQ ID NO:8552715 bpCG56383-02, DNA SequenceORF Start: ATG at 34ORF Stop: TAA at 2566SNP Pos: 989SNP Change: C to TTGCCTGCACTGCAAGTGTCCCCAGGAGGAGCACATGGTGACAGTGATGCCGCTGGAGATGGAGAAGACCATCAGCAAACTCATGTTTGACTTTCAGAGGAACTCGACCTCAGATGATGACTCAGGCTGTGCTTTGGAAGAGTATGCCTGGGTCCCGCCGGGTCTGAAGCCTGAACAGGTACACCAGTACTATAGCTGTCTCCCAGAAGAGAAAGTCCCTTATGTCAACAGTCCTGGAGAGAAACTGCGAATCAAGCAGCTACTACACCAGCTGCCGCCACATGACAATGAGGTAAGGTATTGCAACTCCCTGGATGAGGAAGAGAAGAGGGAGCTGAAGCTTTTCAGCAGCCAGAGGAAACGCGAAAACTTGGGCCGCGGGAATGTCAGGCCTTTCCCAGTCACCATGACAGGAGCTATTTGTGAACAGTGCGGAGGCCAGATCAATGGTGGAGACATCGCTGTGTTTGCGTCACGCGCTGGCCACGGCGTTTGCTGGCACCCGCCGTGCTTCGTATGCACTGTCTGCAATGAGCTCCTGGTGGATCTGATCTACTTTTACCAAGATGGGAAGATATACTGTGGCAGGCACCATGCTGAGTGCCTGAAGCCGCGCTGTGCTGCCTGCGATGAGATCATCTTTGCAGATGAATGCACAGAAGCTGAGGGGCGACACTGGCACATGAAACACTTTTGCTGCTTCGAGTGTGAGACAGTGCTGGGCGGCCAGCGCTACATCATGAAGGAGGGAAGACCCTACTGTTGCCACTGCTTCGAGTCCTTGTATGCAGAATATTGTGACACCTGTGCCCAACATATAGGTATCGACCAAGGTCAAATGACCTATGATGGCCAACACTGGCATGCCACTGAGACCTGTTTCTGCTGTGCTCACTGCAAGAAATCCCTCCTGGGGCGGCCATTCCTCCCGAAGCAGGGCCAGATATTCTGCTCACGGGCCTGCAGTGCTGGGGAAGACCCCAATGGTTTTGACTCCTCTGATTCCGCCTTCCAGAACGCCAGGGCCAAGGAGTCCCGGCGCAGTGCCAAAATTGGCAAGAACAAGGGCAAGACGGAGGAGCCCATGCTGAACCAGCACAGCCAGCTGCAAGTGAGTTCTAACCGGCTGTCAGCCGACGTAGACCCCCTGTCACTGCAGATGGACATGCTCAGCCTGTCCAGCCAGACACCCAGCCTCAACCGGGACCCCATCTGGAGGAGCCGGGAAGAGCCCTACCATTATGGGAACAAGATGGAGCAGAACCAGACCCAGAGCCCTCTGCAGCTCCTCAGCCAGTGCAACATCAGAACTTCCTACAGTCCAGGAGGGCAAGGGGCTGGGGCCCAGCCCGAAATGTGGGGCAAGCACTTCAGCAACCCCAAAAGGAGCTCGTCACTGGCCATGACAGGACATGCTGGCAGCTTCATCAAGGAATGCCGAGAAGACTATTACCCGGGGAGGCTGAGATCTCAGGAGAGCTACAGTGATATGTCTAGTCAGAGTTTCAGTGAGACCCGAGGCAGCATCCAAGTCCCCAAATATGAGGAGGAAGAGGAAGAGGAAGGGGGCTTGTCCACTCAGCAGTGTCGGACCCGTCATCCCATCAGTTCCCTGAAATACACAGAGGACATGACGCCCACAGAGCAGACCCCTCGGGGCTCCATGGAATCCCTGGCCCTGTCTAATGCAACAGGTCTCTCTGCTGATGGTGGTGCCAAGCGCCAGGAGCACCTATCCCGATTTTCCATGCCTGACCTCAGCAAAGACTCTGGAATGAATGTGTCTGAGAAGCTGAGCAACATGGGCACTCTTAACTCGTCCATGCAGTTCCGGAGCGCAGAGTCAGTTCGCAGCCTGCTCTCTGCCCAGCAGTACCAGGAGATGGAGGGAAACCTCCACCAGCTCAGCAACCCCATTGGCTACAGAGACCTGCAGTCCCACGGAAGGATGCATCAGAGCTTTGATTTTGATGGAGGGATGGCGGGCAGCAAGCTGCCAGGGCAGGAGGGCGTGAGGATCCAGCCCATGAGTGAACGCACCCGGAGAAGAGCTACTTCACGCGACGACAACCGCCGTTTCCGACCTCACAGGTCCAGGCGTTCCCGACGCTCTCGCTCCGACAACGCCCTCCACCTGGCCAGCGAACGCGAGGCCATCTCCCGGTTAAAAGATAGGCCCCCTCTGAGAGCCAGGGAGGACTATGACCAATTTATGCGCCAGCGGAGCTTCCAGGAGAGCATGGGGCATGGGTCCCGGAGGGACCTGTACGGCCAGTGCCCTAGGACTGTGTCGGACCTGGCTTTGCAGAATGCCTTTGGGGACCGCTGGGGACCCTACTTCGCCGAGTATGATTGGTGTTCCACCTGCTCCTCCTCTTCAGAGTCTGACAACGAGGGCTATTTCCTAGGAGAACCCATCCCCCAGCCAGCGCGCCTGCGATACGTCACAAGCGATGAGCTGCTGCACAAATACAGCTCCTACGGCCTCCCCAAATCTTCCACATTAGGTGGCAGAGGACAGTTGCACAGCAGGAAAAGACAGAAGAGCAAAAACTGTATCATTTCTTAATATGATTGGGATCAGGGAATGGGAGAAGATGGGAGCTAAGAATGTAAAGTCAGAAACTTGCACTGTTTTAAATGTTAAAGCGCTTTTGGGGGTGGCTTATGGGGGAGAAAAGGGAAAATGCTGTCAGTAGATGGAGGCAAGGTTACANOV36c, SNP13382513 ofSEQ ID NO:856844 aaMW at 95673.9 kDCG56383-02, Protein SequenceSNP Pos: 319SNP Change: Ser to PheMVTVMPLEMEKTISKLMFDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSCLPEEKVPYVNSPGEKLRIKQLLHQLPPHDNEVRYCNSLDEEEKRELKLFSSQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPPCFVCTVCNELLVDLIYFYQDGKIYCGRHHAECLKPRCAACDEIIFADECTEAEGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCHCFESLYAEYCDTCAQHIGIDQGQMTYDGQHWHATETCFCCAHCKKSLLGRPFLPKQGQIFCSRACSAGEDPNGFDSSDSAFQNARAKESRRSAKIGKNKGKTEEPMLNQHSQLQVSSNRLSADVDPLSLQMDMLSLSSQTPSLNRDPIWRSREEPYHYGNKMEQNQTQSPLQLLSQCNIRTSYSPGGQGAGAQPEMWGKHFSNPKRSSSLAMTGHAGSFIKECREDYYPGRLRSQESYSDMSSQSFSETRGSIQVPKYEEEEEEEGGLSTQQCRTRHPISSLKYTEDMTPTEQTPRGSMESLALSNATGLSADGGAKRQEHLSRFSMPDLSKDSGMNVSEKLSNMGTLNSSMQFRSAESVRSLLSAQQYQEMEGNLHQLSNPIGYRDLQSHGRMHQSFDFDGGMAGSKLPGQEGVRIQPMSERTRRRATSRDDNRRFRPHRSRRSRRSRSDNALHLASEREAISRLKDRPPLRAREDYDQFMRQRSFQESMGHGSRRDLYGQCPRTVSDLALQNAFGDRWGPYFAEYDWCSTCSSSSESDNEGYFLGEPIPQPARLRYVTSDELLHKYSSYGLPKSSTLGGRGQLHSRKRQKSKNCIIS


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 36B.

TABLE 36BComparison of the NOV36 protein sequences.NOV36aMVTVMPLEMEKTISKLMFDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSCLPEEKNOV36b------------------------------------------------------------NOV36aVPYVNSPGEKLRIKQLLHQLPPHDNEVRYCNSLDEEEKRELKLFSSQRKRENLGRGNVRPNOV36b------------------------------------------------------------NOV36aFPVTMTGAICEQCGGQINGGDIAVFASRAGHGVCWHPPCFVCTVCNELLVDLIYFYQDGKNOV36b------------------------------------------------------------NOV36aIYCGRHHAECLKPRCAACDEIIFADECTEAEGRHWHMXHFCCFECETVLGGQRYIMKEGRNOV36b-----------------------------------------------------------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-----------------------------------------NOV36aSAESVRSLLSAQQYQEMEGNLHQLSNPIGYRDLQSHGRMHQSFDFDGGMAGSKLPGQEGVNOV36b------------------------------------------------------------NOV36aRIQPMSERTRRRATSRDDNRRFRPHRSRRSRRSRSDNALHLASEREAISRLKDRPPLRARNOV36b------------------------------------------------------------NOV36aEDYDQFMRQRSFQESMGHGSRRDLYGQCPRTVSDLALQNAFGDRWGPYFAEYDWCSTCSSNOV36b------------------------------------------------------------NOV36aSSESDNEGYFLGEPIPQPARLRYVTSDELLHKYSSYGLPKSSTLGGRGQLHSRKRQKSKNNOV36b------------------------------------------------------------NOV36aCIISNOV36b----NOV36a(SEQ ID NO: 852)NOV36b(SEQ ID NO: 854)


Further analysis of the NOV36a protein yielded the following properties shown in Table 36C.

TABLE 36CProtein Sequence Properties NOV36aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 11; pos. chg 1; neg. chg 2H-region:length 3; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −10.32possible cleavage site: between 26 and 27>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 0PERIPHERALLikelihood = 8.54 (at 365)ALOM score: −1.17 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):5.58Hyd Moment(95):3.29G content:0D/E content:2S/T content:1Score: −7.15Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PHRSRRS (4) at 684bipartite: nonecontent of basic residues: 12.6%NLS Score: −0.13KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: foundRIKQLLHQL at 72VAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: CaaX motif in the C-terminus: CIISif X is S, A, or M, it will be farnesylatedotherwise, it will be geranylgeranylatedmemYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs:Leucine zipper pattern (PS00029): *** found *** LQVSSNRLSADVDPLSLQMDML at 358LIM domain signature (PS00478): *** found *** CEQCGGQINGGDIAVFASRAGHGVCWHPPCFV CTVCNELL at 130checking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):73.9%: nuclear 8.7%: mitochondrial 8.7%: plasma membrane 8.7%: cytoplasmic>> prediction for CG56383-02 is nuc (k = 23)


A search of the NOV36a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 36D.

TABLE 36DGeneseq Results for NOV36aNOV36aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #,Date]ResiduesRegionValueABG07025Novel human diagnostic protein49 . . . 844 721/807 (89%)0.0#7016 - Homo sapiens, 795 aa.1 . . . 795737/807 (90%)[WO200175067-A2, 11-OCT-2001]ABB82161Human NOV6b protein sequence -1 . . . 554 554/554 (100%)0.0Homo sapiens, 559 aa.1 . . . 554 554/554 (100%)[WO200270660-A2, 12-SEP-2002]ABU11832Human MDDT polypeptide SEQ ID5 . . . 844429/851 (50%)0.0779 - Homo sapiens, 839 aa.9 . . . 839542/851 (63%)[WO200279449-A2, 10-OCT-2002]ABU11569Human MDDT polypeptide SEQ ID5 . . . 844429/851 (50%)0.0516 - Homo sapiens, 839 aa.9 . . . 839542/851 (63%)[WO200279449-A2, 10-OCT-2002]ABG08405Novel human diagnostic protein49 . . . 350  302/302 (100%)0.0#8396 - Homo sapiens, 311 aa.1 . . . 302 302/302 (100%)[WO200175067-A2, 11-OCT-2001]


In a BLAST search of public sequence databases, the NOV36a protein was found to have homology to the proteins shown in the BLASTP data in Table 36E.

TABLE 36EPublic BLASTP Results for NOV36aNOV36aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueAAH50793Similar to prickle-like 1 (Drosophila) -1 . . . 844803/845 (95%)0.0Mus musculus (Mouse), 879 aa35 . . . 879 826/845 (97%)(fragment).AAP33399Prickle2 - Brachydanio rerio5 . . . 844569/852 (66%)0.0(Zebrafish) (Danio rerio), 840 aa.1 . . . 840664/852 (77%)Q96MT3Hypothetical protein FLJ31937 -5 . . . 844428/851 (50%)0.0Homo sapiens (Human), 831 aa.1 . . . 831541/851 (63%)Q90WV2LIM protein prickle b - Xenopus5 . . . 844413/861 (47%)0.0laevis (African clawed frog), 866 aa.35 . . . 866 538/861 (61%)AAP33398Prickle1 - Brachydanio rerio7 . . . 844387/853 (45%)0.0(Zebrafish) (Danio rerio), 793 aa.9 . . . 793499/853 (58%)


PFam analysis predicts that the NOV36a protein contains the domains shown in the Table 36F.

TABLE 36FDomain Analysis of NOV36aIdentities/PfamNOV36aSimilarities forDomainMatch Regionthe Matched RegionExpect ValueLIM130 . . . 19219/66 (29%)2.9e−1143/66 (65%)LIM195 . . . 25217/62 (27%)3.9e−1345/62 (73%)LIM255 . . . 31516/66 (24%)0.02234/66 (52%)


Example 37

The NOV37 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 37A.

TABLE 37ANOV37 Sequence AnalysisNOV37a, CG56449-04SEQ ID NO: 857877 bpDNA SequenceORF Start: ATG at 25ORF Stop: TAG at 535CCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCCGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAACGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGGAAGAGAGGCGTCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGNOV37a, CG56449-04Protein SequenceSEQ ID NO: 858170 aaMW at 17123.1kDMAPASAPLAAGAPAVPRPALPACTATTVGIPASARTEGPVTLSQACEHPCPPGFHGAGRQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREGGPLRLPENPSLAQGSAGTLPASSRPTSRSGGPARHNOV37b, CG56449-09SEQ ID NO: 8595172 bpDNA SequenceORF Start: ATG at 16ORF Stop: TAG at 4798GCACCGGCGCGCACGATGTCGTTCCTTGAAGAGGCGAGGGCAGCGGGGCGCGCGGTGGTCCTGGCGTTGGTGCTGCTGCTGCTCCCCGCCGTGCCCGTGGGCGCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAGGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGAACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGACGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGCTGAATGCAGCGCCAGCCTCTGTTTTCACGGTGGCCGTTGTGTGCCAGGCTCAGCCCAGCCGTGTCACTGTCCCCCCGGCTTCCAGGGACCCCGCTGTCAGTATGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCCTGGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCCGGGGCCTCGTCAGTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGACCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATAACTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGAAACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGAAGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTAACGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCGGGGCCTGTGCCACGCCAGCAAGCGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAAAGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGAATTCCACGAACACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGGGGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGGGCCGGGGGGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACTCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGGAAGAGAGGCGCCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGTCACAGTGCAGGGTGCAGTCACAGTGCAGCGTGCNOV37b, CG56449-09Protein SequenceSEQ ID NO: 8601594 aaMW at 166431.4kDMSFLEEARAAGRAVVLALVLLLLPAVPVGASVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTVPVWPAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGCLSAECSASLCFHGGRCVPGSAQPCHCPPGFQGPRCQYDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCLAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVTGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASGACATPASGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREGGPLRLPENPSLAQGSAGTLPASSRPTSRSGGPARHNOV37c, 191887507SEQ ID NO: 861522 bpDNA SequenceORF Start: at 2ORF Stop: at 521GGATCCGTGCCTCGCTGGTCCACCGCTCATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGCTCGAGNOV37c, 191887507Protein Sequence SEQ ID NO: 862173 aaMW at 17358.4kDDPCLAGPPLMAPASAPLAAGAPAVPRPALPACTATTVGIPASARTEGPVTLSQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREGGPLRLPENPSLAQGSAGTLPASSRPTSRSNOV37d, 316351371SEQ ID NO: 8634255 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGTCGTTCCTTGAAGAGGCGAGGGCAGCGGGGCGCGCGGTGGTCCTGGCGTTGGTGCTGCTGCTGCTCCCCGCCGTGCCCGTGGGCGCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGAACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGACGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGCTGAATGCAGCGCCGGCCTCTGTTTTCACGGTGGCCGTTGTGTGCCAGGCTCAGCCCAGCCGTGTCACTGTCCCCCCGGCTTCCAGGGACCCCGCTGTCAGTATGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCCTGGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGGATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCGATGTCGGCGACTGTGCAGACAGCCCGTGCTGCCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGTGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCCCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGTCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGACTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCACCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACGTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCACCGGGGAGGACTGGGGAAGACTGTGAGGCAGATTGTCCCGAGGGCCGCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCAGCACGCTGCCCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGACTTCGTCGGCAGCCGCTGCCAGGACGTGTGCCCAGCAGGCTGGTATGGTCCCAGCTGCCAGACAAGGTGCTCTTGTGCCAATGATGGGCACTGCCACCCAGCCACCGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCTGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAAGTGCCTCCCCCGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCNOV37d, 316351371Protein SequenceSEQ ID NO: 8641418 aaMW at 148398.2kDTGSTMSFLEEARAAGRAVVLALVLLLLPAVPVGASVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGCLSAECSAGLCFHGGRCVPGSAQPCHCPPGFQGPRCQYDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCLAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIDVGDCADSPCCQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSPLEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNETCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLTCPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEADCPEGRWGLGCQEICPACQHAARCDPETGACLCLPDFVGSRCQDVCPAGWYGPSCQTRCSCANDGHCHPATGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQQCPQGHFGPGCEQLCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAEKCLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPCLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQGCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCETACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRPGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREGGPLRLPENPSLAQGSAGTLPASSRPTSRSGGPARHGTGNOV37e, 316935396SEQ ID NO: 8655000 bpDNA SequenceORF Start: at 28ORF Stop: TGA at 4987TGCTGTTACAGGTGGAGGGCAGTGTAGTCTGAGCAGTACTCGTTGCTGCCGCGCGCGCCACCAGACATAATAGCTGACAGACTAACAGACTGTTCCTTTCCATGGGTCTTTTCTGCAGTCACCGTCCTTGACACGAAGCTTTCTAGAAGATCTTCGCGAGGATCCACCATGTCGTTCCTTGAAGAGGCGAGGGCAGCGGGGCGCGCGGTGGTCCTGGCGTTGGTGCTGCTGCTGCTCCCCGCCGTGCCCGTGGGCGCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACGTATTCCCCGGGCTCGAGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCACCACCATCACCATTGAGTTTAATTCATNOV37e, 316935396Protein SequenceSEQ ID NO: 8661653 aaMW at 173369.0kDSEQYSLLPRAPPDIIADRLTDCSFPWVFSAVTVLDTKLSRRSSRGSTMSFLEEARAAGRAVVLALVLLLLPAVPVGASVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTTPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHTACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAETYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRhSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREAGTLPASSRPTSRSGGPARHVFPGLEGKPIPNPLLGLDSTRTGHHHHHHNOV37f, 317004318SEQ ID NO: 8675005 bpDNA SequenceORF Start: at 126ORF Stop: TGA at 4986AACGGGTGGAGGGCAGTGTAGTCTGAGCAGTACTCGTTGCTGCCGCGCGCGCCACCAGACATAATAGCTGACAGACTAACAGACTGTTCCTTTCCATGGGTCTTTTCTGCAGTCACCGTCCTTGACACGAAGCTCTAGCCACCATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCGGCCCAGCCGGCCAGGCGCGCGCGCCGTACGAAGCTTTCGCGAGGATCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGaCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACGAATTCCCCGGGCTCGAGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCACCACCATCACCATTGAGTTTAATTCATTGATTTNOV37f, 317004318Protein SequenceSEQ ID NO: 8681620 aaMW at 169975.0kDHEALATMETDTLLLWVLLLWVPGSTGDAAQPARRARRTKLSRGSSVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREAGTLPASSRPTSRSGGPARHEFPGLEGKPIPNPLLGLDSTRTGHHHHHHNOV37g, CG56449-01SEQ ID NO: 8697337 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 4213ATGCCCATGGGACATTCTGACAGGTGGTCTTGGCGTCTCCTGAGGCTGGCACTGCCACTCCCAGTCTGGTTGCCGGCTGGGGGTGGCCGAGGCGCTGACTCTCCATGTCTCTGTTCCAGGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTAAAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGAACGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTAACTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGAAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCGCTGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAAAGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAAGCGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCACTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGAGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGAATCGCATGGCCGCTGGAAGAGAGGCGCCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGCAGGTGCAGGCACAGGGCCACTGTCCTCCAGGCAGGCTTTTTGGTGCTAGGCCCTGGGACTGGAAGTCGCCCAGCCCGTATTTATGTAAAGGTATTTATGGGCCACTGCACATGCCCGCTGCAGCCCTGGGATCAGCTGGAAGCTGCCTGTCATCTCCTGCCCAATCCCCAGAAACCCTGATTCAGGTCTGCAGGCTCCTGCGGGCTCACCAGGCTGCTGGCTCCGGTACCATGTACCTAAAGGTAAAAAAAGGAGCAGGCAACCTCCTCGTGGCCTGTGTGTTTGCTGTGTTACGTGGACTCTGTGTGGGCTCCTCCCTGGGGCCCGGCCAGCATAACGGTGCACCCAGGGACCTCCCAGTGCACCCGGGGCCCTTTGCAGGGGTGGGGGTGCCACACAAGTGAAGAAGTTGGGACTCATCTCAGTTCCCAGTGCTATTGAGGAGAACGCTGGGGCTGCATTCATTACCGCTGAGACCCAGAGACTGGCTGTTCCCAGAGAATGGCCCAGGGGGAGGAGAACTGGTGTAAAGGGGCAACCTGGACTGAGGCCGAACTCCCTTGGGCTCACCCCACCCACCCCTACCTGAGCATCAGCAGTGGGGGGAGGGCAGCATCGCAGGGGCAGGGACTCCCTGGGTGAGGACAGACCAGCCCTCCCGAGCACCTGGCACTCATGGGCTGAGGCTGACTTCTCCTGGAAGAAGGGCCCAGAGTGGAAGGAAGAGGCAGAGGCTAGAGGTGGTGGCTGGGGGCTCCTCTGCAGAGTGGGGTGGCCAATGGAGAGGGCTGCACTCACACCGCACATAGGAACTCTCTCTCCCTTAAGAAGGCCCCCTTAGGGTCTGGGCTGCCGCCCCCATCACCCTAAACCAGCCAAAGGTAGCTGAGGCCCCAGGGCAGACAATTTCACCAGCAGGANGAGGAGGAGTCCAGTGAGCTTGGTTGCTCACAGACAGCAAGGGAGCTGTCACAGAGGAAGCTGATGAATGGACCGCTGTGGGGAGACTTTAAAGTAGAACAGTGATAAGGGAGGGCAGGATGGTGGGGATGCAGAAGCAGCAGCCAGAGAGAGACGGACTGGGGTGCAGACGGAGTGTGGAAAACGCATACCTTGAAATGAAGCATCCAGCAGATGGGGTGAGTGGATACAGCTCAGGAGATTCTCCCAGGAATAGCAGGGAGGCGTAAAGAGAGACAACGTACAGAGATAGATGAATGGAAATGGGTAAGGGAGGTGTTCATTCACATCCATCTAACTGCAAAATACAAAAGTAAGAAGTCATTGACATGAAGCAACGACGACCAAGACGTTCTCAGATCTAAAGGTGAATGATCTCAGTCAGCCTGGAAATGCACAAGGTGGAAAAATAACATAAAAAAGCCATAAGACCTTGAAGAACATCAATGTCAAAGATAAATTCTAAAGTCCCAGAGAAAAAAGAATGGGAATCAAATTGACCTCAGACTATACGTGAGAAACACGGAGAGCCAGAAAACTGTGATGTTCCATCCTCAGAGTTTGAAGGAAATATTTGAAGGCTGAATTTTACATCCAGCTAAACTATCAAAGGCATGCAAAGTCCATGTTATTCTTAGGCCTTCAAGGCCTCGGCCATTTTTCTACAGAAAAGCCTGATTTTAAAATGCTCTTAGAGACGTTCTCCAGCCAGAAGAGAAAGAAGCCAGGAGGGTGCTCTGAGATATTCAGTCACCACAGTTCCCAAATGGCCTAGGAATTCAGAGAGTCAGAATATCACCATTACTCCCCAATGGGAACCCCCGACAGTCTCAGCATGGTGTGAGGGTGTGGACGGGGGGCCTGGCAGGTACCAATCACTCATCCCGCTCAGTGAAGACACAGTGTTCAGCTACGGAAGCCATAAGGCAGGCCGAGCTTCTGCCCATCCGGAGGAAATCTCAGCTATCCAACGGCGGTCAGGAGCAGAGGAAAATAAAGCAGAATAACTAGAAAACACGCTCACAGATCCTAATGTTAACGGTTACAAATGACGACGGAAAAACAAACTCCTGACCATATATTATATAGTTTCAAGCAGCAAGAAGGAGGATATTGAACATTCTCAACACACATAATAAACGCTTGAGATGATGATATGCTCATTACCCTGATTTGATCACTAGACATACCATGTATCAAAACATCACTGTGTATCCGATGAATATCTACAATTATTGTCAATTAAAACATCATTAAAAAACAANOV37g, CG56449-01Protein SequenceSEQ ID NO: 8701404 aaMW at 147886.8kDMPMGHSDRWSWRLLRLALPLPVWLPAGGGRGADSPCLCSRPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNTPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQPACDTGHWGPDCSHPCNCSAGHGSCDASGLCLCEAGYVTGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASKRQLLLWPGLDGAALRAGLSPWALRSRLPSGVLLPQQQHVNOV37h, CG56449-02SEQ ID NO: 8717319 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 4195ATGCCCATGGGACATTCTGACAGGTGGTCTTGGCGTCTCCTGAGGCTGGCACTGCCACTCCCAGTCTGGTTGCCGGCTGGGGGTGGCCGAGGCGCTGACTCTCCATGTCTCTGTTCCAGGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGAACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGACGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGCTGAATGCAGCGCCAGCCTCTGTTTTCACGGTGGCCGTTGTGTGCCAGGCTCAGCCCAGCCGTGTCACTGTCCCCCCGGCTTCCAGGGACCCCGCTGTCAGTATGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCCTGGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAAGCGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGCAACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGGAAGAGAGGCGCCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGCAGGTGAGGCACAAGGGCCACTGTCCTCCAGGCAGGCTTTTTGGTGCTAGGCCCTGGGACTGGAAGTCGCCCAGCCCGTATTTATGTAAAGGTATTTATGGGCCACTGCACATGCCCGCTGCAGCCCTGGGATCAGCTGGAAGCTGCCTGTCATCTCCTGCCCAATCCCCAGAAACCCTGATTCAGGTCTGCAGGCTCCTGCGGGCTCACCAGGCTGCTGGCTCCGGTACCATGTAAACCTAGGAAGGTAAAGGAGCAGGCAACCTCCTCGTGGCCTGTGTGTTTGCTGTGTTACGTGGACTCTGTGTGGGCTCCTCCCTGGGGCCCGGCCAGCATAACGGTGCACCCAGGGACCTCCCAGTGCACCCGGGGCCCTTTGCAGGGGTGGGGGTGCCACACAAGTGAAGAAGTTGGGACTCATCTCAGTTCCCAGTGCTATTGAGGAGAACGCTGGGGCTGCATTCATTACCGCTGAGACCCAGAGACTGGCTGTTCCCAGAGAATGGCCCAGGGGGAGGAGGGCTGGTGTGGAGGGGCAACCTGGACTGAGGCCGAACTCCCTTGGGCTCACCCCACCCACCCCTACCTGAGCATCAGCAGTGGGGGGAGGGCAGCATCGCAGGGGCAGGGACTCCCTGGGTGAGGACAGACCAGCCCTCCCGAGCACCTGGCACTCATGGGCTGAGGCTGACTTCTCCTGGAAGAAGGGCCCAGAGTGGAAGGAAGAGGCAGAGGGTAGAGGTGGTGGCTGGGGGCTCCTCTGCAGAGTGGGGTGGCCAATGGAGAGGGCTGCACTCACACCGCAACATAGGACTCTCTCTCCCTTAAGAAGGCCCCCTTAGGGTCTGGGCTGCCGCCCCCATCACCCTAAAACCAGCCAAGGTAGCTGAGGCCCCAGGGCAGACAATTTCACCAGCAGGANGAGGAGGAGTCCAGTGAGCTTGGTTGCTCACAGACAGCAAGGGAGCTGTCACAGAGGAAGCTGATGAATGGACCGCTGTGGGGAGACTTTAAAGTAGAACAGTGATAAGGGAGGGCAGGATGGTGGGGATGCAGAAGCAGCAGCCAGAGAGAGACGGACTGGGGTGCAGACCGAGTGTGGAAAACGCATACCTTGAAATGAAGCATCCAGCAGATGGGGTGAGTGGATACAGCTCAGGAGATTCTCCCAGGAATAGCAGGGAGGCGTAAAGAGAGACAACGTACAGAGATAGATGAATGGAAATGGGTAAGGGAGGTGTTCATTCACATCCATCTAACTGCAAAATACAAAAGTAAGAAGTCATTGACATGAAGCAACGACGACCAAGACGTTCTCAGATCTAAAGGTGAATGATCTCAGTCAGCCTGGAAATGCACAAGGTGCAAAAATAACATAAAAAAGCCATAAGACCTTGAAGAACATCAATGTCAAAGATAAATTCTAAAGTCCCAGAGAAAAAAGAATGGGAATCAAATTGACCTCAGACTATACGTGAGAAACACGGAGAGCCAGAAAACTGTGATGTTCCATCCTCAGAGTTTGAAGGAAATATTTGAAGGCTGAATTTTACATCCAGCTAAACTATCAAAGGCATGCAAAGTCCATGTTATTCTTAGGCCTTCAAGGCCTCGGCCATTTTTCTACAGAAAAGCCTGATTTTAAAATGCTCTTAGAGACGTTCTCCAGCCAGAAGAGAAAGAAGCCAGGAGGGTGCTCTGAGATATTCAGTCACCACAGTTCCCAAATGGCCTAGGAATTCAGAGAGTCAGAATATCACCATTACTCCCCAATGGGAACCCCCGACAGTCTCAGCATGGTGTGAGGGTGTGGACGGGGGGCCTGGCAGGTACCAATCACTCATCCCGCTCAGTGAAGACACAGTGTTCAGCTACGGAAGCCATAAGGCAGGCCGAGCTTCTGCCCATCCGGAGGAAATCTCAGCTATCCAACGGCGGTCAGGAGCAGAGGAAAATAAAGCAGAATAACTAGAAAACACGCTCACAGATCCTAATGTTAACGGTTACAAATGACGACGGAAAAACAAACTCCTGACCATATATTATATAGTTTCAAGCAGCAAGAAGGAGGATATTGAACATTCTCAACACACATAATAAACGCTTGAGATGATGATATGCTCATTACCCTGATTTGATCACTAGACATNCCATGTATCAAAACATCACTGTGTATCCGATCAATATCTACAATTATTGTCAATTAAAAACATCATTAAAAACAANOV37h, CG56449-02Protein SequenceSEQ ID NO: 8721398 aaMW at 147208.2kDMPMGHSDRWSWRLLRLALPLPVWLPAGGGRGADSPCLCSRPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGCLSAECSASLCFHGGRCVPGSAQPCHCPPGFQGPRCQYDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCLAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVPRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHTSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPFCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASKRQLLLWPGLDGAALRAGLSPWALRSRLPSGVLLPQQQHVNOV37i, CG56449-03SEQ ID NO: 8734733 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 4351ATGCCCATGGGACATTCTGACAGGTGGTCTTGGCGTCTCCTGAGGCTGGCACTGCCACTCCCAGTCTGGTTGCCGGCTGGGGGTGGCCGAGGCGCTGACTCTCCATGTCTCTGTTCCAGGCCCCACGTGTGTGCTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGAACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGACGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGCTGAATGCAGCGCCAGCCTCTGTTTTCACGGTGGCCGTTGTGTGCCAGGCTCAGCCCAGCCGTGTCACTGTCCCCCCGGCTTCCAGGGACCCCGCTGTCAGTATGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCCTGGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCTGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACGTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCACCGGGGAGGACTGGGGAAGACTGTGAGGCAGATTGTCCCGAGGGCCGCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCAGCACGCTGCCCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTCGTCGGCAGCCGCTGCCAGGACGTGTGCCCAGCAGGCTGGTATGGTCCCAGCTGCCAGACAAGGTGCTCTTGTGCCAATGATGGGCACTGCCACCCAGCCACCGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGCAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCTGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGTAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGAAGGAGTGCCCCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGTGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGGCTGGGGACAAGTGTCAGAGCCCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCACTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGGGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTCTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGGAAGAGAGGCGCCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGCAGGTGCAGGCACAGGGCCACTGTCCTCCAGGCAGGCTTNOV37i, CG56449-03Protein SequenceSEQ ID NO: 8741450 aaMW at 152213.4kDMPMGHSDRWSWRLLRLALPLPVWLPAGGGRGADSPCLCSRPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGCLSAECSASLCFHGGRCVPGSAQPCHCPPGFQGPRCQYDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCLAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGILACECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECELGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEADCPEGRWGLGCQEICPACQHAARCDPETGACLCLPGFVGSRCQDVCPAGWYGPSCQTRCSCANDGHCHPATGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQQCPQGHFGPGCEQLCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAETCPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLLACEKECPPRDVPAGCRHSGGCLNGGCDPHTGRCLCPAGWAGDKCQSPCLRGWFGEACAQHCSCPPGAACHHVTGACRCPPGFTGSGCEQGCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTGEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCNCVDGYMGPTCREGGPLRLPENPSLAQGSAGTLPASSRPTSRSGGPARHNOV37j, CG56449-05SEQ ID NO: 875522 bpDNA SequenceORF Start: ATG at 29ORF Stop: at 515GGATCCGTGCCTCGCTGGTCCACCGCTCATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGCTCGAGNOV37l, CG56449-05Protein SequenceSEQ ID NO: 876162 aaMW at 16251.2kDMAPASAPLAAGAPAVPRPALPACTATTVGIPASARTEGPVTLSQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREGGPLRLPENPSLAQGSAGTLPASSRPTSNOV37k, CG56449-06SEQ ID NO: 8777334 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 4210ATGCCCATGGGACATTCTGACAGGTGGTCTTGGCGTCTCCTGAGGCTGGCACTGCCACTCCCAGTCTGGTTGCCGGCTGGGGGTGGCCGAGGCGCTGACTCTCCATGTCTCTGTTCCAGGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAAGCGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGQAAGAGAGGCGCCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGCAGGTGCAGGCACAGGGCCACTGTCCTCCAGGCAGGCTTTTTGGTGCTAGGCCCTGGGACTGGAAGTCGCCCAGCCCGTATTTATGTAAAGGTATTTATGGGCCACTGCACATGCCCGCTGCAGCCCTGGGATCAGCTGGAAGCTGCCTGTCATCTCCTGCCCAATCCCCAGAAACCCTGATTCAGGTCTGCAGGCTCCTGCGGGCTCACCAGGCTGCTGGCTCCGGTACCATGTAAACCTAGGAAGGTAAAGGAGCAGGCAACCTCCTCGTGGCCTGTGTGTTTGCTGTGTTACGTGGACTCTGTGTGGGCTCCTCCCTGGGGCCCGGCCAGCATAACGGTGCACCCAGGGACCTCCCAGTGCACCCGGGGCCCTTTGCAGGGGTGGGGGTGCCACACAAGTGAAGAAGTTGGGACTCATCTCAGTTCCCAGTGCTATTGAGGAGAACGCTGGGGCTGCATTCATTACCGCTGAGACCCAGAGACTGGCTGTTCCCAGAGAATGGCCCAGGGGGAGGAGGGCTGGTGTGGAGGCGCAACCTGGACTGAGGCCGAACTCCCTTGGGCTCACCCCACCCACCCCTACCTGAGCATCAGCAGTGGGGGGAGGGCAGCATCGCAGGGGCAGGGACTCCCTGGGTGAGGACAGACCAGCCCTCCCGAGCACCTGGCACTCATGGGCTGAGGCTGACTTCTCCTGGAAGAAGGGCCCAGAGTGGAAGGAAGAGGCAGAGGGTAGAGGTGGTGGCTGGGGGCTCCTCTGCAGAGTGGGGTGGCCAATGGAGAGGGCTGCACTCACACCGCAACATAGGACTCTCTCTCCCTTAAGAAGGCCCCCTTAGGGTCTGGGCTGCCGCCCCCATCACCCTAAAACCAGCCAAGGTAGCTGAGGCCCCAGGGCAGACAATTTCACCAGCAGGANGAGGAGGAGTCCAGTGAGCTTGGTTGCTCACAGACAGCAAGGGAGCTGTCACAGAGGAAGCTGATGAATGGACCGCTGTGGGGAGACTTTAAAGTAGAACAGTGATAAGGGAGGGCAGGATGGTGGGGATGCAGAAGCAGCAGCCAGAGAGAGACGCACTGGGGTGCAGACGGAGTGTGGAAAACGCATACCTTGAAATGAAGCATCCAGCAGATGGGGTGAGTCGATACAGCTCAGGAGATTCTCCCAGGAATAGCAGGGAGGCGTAAAGAGAGACAACGTACAGAGATAGATGAATGGAAATGGGTAAGGGAGGTGTTCATTCACATCCATCTAACTGCAAAATACAAAAGTAAGAAGTCATTGACATGAAGCAACGACGACCAAGACGTTCTCAGATCTAAAGGTGAATGATCTCAGTCAGCCTGGAAATGCACAAGGTGGAAAAATAACATAAAAAAGCCATAAGACCTTGAAGAACATCAATGTCAAAGATAAATTCTAAAGTCCCAGAGAAAAAAGAATGGGAATCAAATTGACCTCAGACTATACGTGAGAAACACGGAGAGCCAGAAAACTGTGATGTTCCATCCTCAGAGTTTGAAGGAAATATTTGAAGGCTGAATTTTACATCCAGCTAAACTATCAAAGGCATGCAAAGTCCATGTTATTCTTAGGCCTTCAAGGCCTCGGCCATTTTTCTACAGAAAAGCCTGATTTTAAAATGCTCTTAGAGACGTTCTCCAGCCAGAAGAGAAAGAAGCCAGGAGGGTGCTCTGAGATATTCAGTCACCACAGTTCCCAAATGGCCTAGGAATTCAGAGAGTCAGAATATCACCATTACTCCCCAATGGGAACCCCCGACAGTCTCAGCATGGTGTGAGGGTGTGGACGGGGGGCCTGGCAGGTACCAATCACTCATCCCGCTCAGTGAAGACACAGTGTTCAGCTACGGAAGCCATAAGGCAGGCCGAGCTTCTGCCCATCCGGAGGAAATCTCAGCTATCCAACGGCGGTCAGGAGCAGAGGAAAATAAAGCAGAATAACTAGAAAACACGCTCACAGATCCTAATGTTAACGGTTACAAATGACGACGGAAAAACAAACTCCTGACCATATATTATATAGTTTCAAGCAGCAAGAAGGAGGATATTGAACATTCTCAACACACATAATAAACGCTTGAGATGATGATATGCTCATTACCCTGATTTGATCACTAGACATACCATGTATCAAAACATCACTGTGTATCCGATGAATATCTACAATTATTGTCAATTAAAAACATCATTAAAAACAANOV37k, CG56449-06Protein SequenceSEQ ID NO: 8781403 aaMW at 147829.8kDMPMGHSDRWSWRLLRLALPLPVWLPAGGGRGADSPCLCSRPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGIARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEAINGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAETCPAHTYGHNCSQACACFNGASCDPVHGQCHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASKRQLLLWPGLDGAALAAGLSPWALRSRLPSGVLLPQQQHVNOV37i, CG56449-07SEQ ID NO: 8794783 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 3595ATGCCCATGGGACATTCTGACAGGTGGTCTTGGCGTCTCCTGAGGCTGGCACTGCCACTCCCAGTCTGGTTGCCGGCTGGGGGTGGCCGAGGCGCTGACTCTCCATGTCTCTGTTCCAGGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGAACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGACGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGCTGAATGCAGCGCCAGCCTCTGTTTTCACGGTGGCCGTTGTGTGCCAGGCTCAGCCCAGCCGTGTCACTGTCCCCCCGGCTTCCAGGGACCCCGCTGTCAGTATGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCCTGGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCTGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACGTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCACCGGGGAGGACTGGGGAAGACTGTGAGGCAGATTGTCCCGAGGGCCGCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCAGCACGCTGCCCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTCGTCGGCAGCCGCTGCCAGGACGTGTGCCCAGCAGGCTGGTATGGTCCCAGCTGCCAGACAAGGTGCTCTTGTGCCAATGATGGGCACTGCCACCCAGCCACCGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGCAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCTGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGTAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGAAGGAGTGCCCCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCATCCCCACGGGCCCCTCCTAGAAGCCTCCGCAGCCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGCCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGTTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTCCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGGGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTGGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTCCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGGAAGAGAGGCGCCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGCAGGTGCAGGCACAGCGCCACTGTCCTCCAGGCAGGCTTNOV371, CG56449-07Protein SequenceSEQ ID NO: 8801198 aaMW at 126170.7kDMPMGHSDRWSWRLLRLALPLPVWLPAGGGRGADSPCLCSRPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGCLSAECSASLCFHGGRCVPGSAQPCHCPPGFQGPRCQYDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCLAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANPNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECELGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEADCPEGRWGLGCQEICPACQHAARCDPETGACLCLPGFVGSRCQDVCPAGWYGPSCQTRCSCANDGHCHPATGHCSCAPGWTGFSCQRACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQQCPQGHFGPGCEQLCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAETCPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEKECPPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSHPHGPLLEASAALIFLQPACGAGLERPVPSAAAARLPLPATTSLGPAAVPLASLAPAASRDVRPGGMGQAVNSCVGVSTGAPVMRPRGPAAAPLGSSGRTATSPVRRAASAPTAPTCVVVGRGRPATLNOV37m, CG56449-08SEQ ID NO: 8814835 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 4732ATGTCGTTCCTTGAAGAGGCGAGGGCAGCGGGGCGCGCGGTGGTCCTGGCGTTGGTGCTGCTGCTGCTCCCCGCCGTGCCCGTGGGCGCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGNOV37m, CG56449-08Protein SequenceSEQ ID NO: 8821577 aaMW at 164962.6kDMSFLEEARAAGRAVVLALVLLLLPAVPVGASVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQAACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPJHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREAGTLPASSRPTSRSGGPARHNOV37n, CG56449-10SEQ ID NO: 8835000 bpDNA SequenceORF Start: ATG at 169ORF Stop: at 4900TGCTGTTACAGGTGGAGGGCAGTGTAGTCTGAGCAGTACTCGTTGCTGCCGCGCGCGCCACCAGACATAATAGCTGACAGACTAACAGACTGTTCCTTTCCATGGGTCTTTTCTGCAGTCACCGTCCTTGACACGAAGCTTTCTAGAAGATCTTCGCGAGGATCCACCATGTCGTTCCTTGAAGAGGCGAGGGCAGCGGGGCGCGCGGTGGTCCTGGCGTTGGTGCTGCTGCTGCTCCCCGCCGTGCCCGTGGGCGCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAaGGCCCGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCACAGAGAAGTTTGTCTGCCTGGATGACTCCTTTGGCCATGACTGCAGCTTGACCTGTGATGACTGCAGGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTGCAATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCACTGTGCGTGCCCAGAGGGCTGGGCCGGCCTGGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGAGCCACCTGTAACCTGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACGTATTCCCCGGGCTCGAGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCACCACCATCACCATTGAGTTTAATTCATNOV37n, CG56449-10Protein SequenceSEQ ID NO: 8841577 aaMW at 164962.6kDMSFLEEARAAGRAVVLALVLLLLPAVPVGASVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGTAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMETVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRIGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGPAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREAGTLPASSRPTSRSGGPARHNOV37o, CG56449-1SEQ ID NO: 8855005 bpDNA SequenceORF Start: at 258ORF Stop: at 4899AACGGGTGGAGGGCAGTGTAGTCTGAGCAGTACTCGTTGCTGCCGCGCGCGCCACCAGACATAATAGCTGACAGACTAACAGACTGTTCCTTTCCATGGGTCTTTTCTGCAGTCACCGTCCTTGACACGAAGCTCTAGCCACCATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGGGTTCCAGGTTCCACTGGTGACGCGGCCCAGCCGGCCAGGCGCGCGCGCCGTACGAAGCTTTCGCGAGGATCCAGCGTTCCGCCGCGGCCCCTGCTCCCGCTGCAGCCCGGCATGCCCCACGTGTGTGCTGAGCAGGAGCTGACCCTGGTGGGCCGCCGCCAGCCGTGCGTGCAGGCCTTAAGCCACACGGTGCCGGTGTGGAAGGCCGGCTGTGGGTGGCAGGCGTGGTGCGTGGGTCATGAGCGGAGGACCGTCTACTACATGGGCTACAGGCAGGTGTATACCACGGAGGCCCGGACCGTGCTCAGGTGCTGCCGAGGGTGGATGCAGCAGCCCGACGAGGAGGGCTGCCTCTCGGATGTGGGTGAGTGTGCCAACGCCAACGGGGGCTGTGCGGGTCGGTGCCGGGACACCGTGGGGGGCTTCTACTGCCGCTGGCCCCCCCCCAGCCACCAGCTGCAGGGTGATGGCGAGACTTGCCAAGATGTGGACGAATGCCGAACCCACAACGGTGGCTGCCAGCACCGGTGCGTGAACACCCCAGGCTCCTACCTCTGTGAGTGCAAGCCCGGCTTCCGGCTCCACACTGACAGCAGGACCTGCGCCATTAACTCCTGCGCCCTGGGCAATGGCGGCTGCCAGCACCACTGTGTCCAGCTCACAATCACTCGGCATCGCTGCCAGTGCCGGCCCGGGTTCCAGCTCCAGGAGGACGGCAGGCATTGTGTCCGTAGAAGCCCGTGTGCCAACAGGAACGGCAGCTGCATGCACAGGTGCCAGGTGGTCCGGGGCCTCGCCCGCTGTGAGTGCCACGTGGGCTATCAGCTAGCAGCGGACGGCAAGGCCTGTGAAGATGTGGACGAATGTGCCGCAGGGCTGGCCCAGTGTGCCCATGGCTGCCTCAACACCCAGGGGTCCTTCAAGTGCGTGTGTCACGCGGGCTATGAGCTGGGCGCCGATGGCCGGCAGTGCTACCGTATTGAGATGGAAATCGTGAACAGCTGTGAGGCCAACAACGGCGGCTGCTCCCATGGCTGCAGCCACACCAGTGCTGGGCCCCTGTGCACCTGTCCCCGCGGCTACGAGCTGGACACAGATCAGAGGACCTGCATCAGATGTCGACGACTGTGCAGACAGCCCGTGCTGCAGCAGGTGTGCACCAACAACCCTGGCGGGTACGAGTGCGGCTGCTACGCCGGCTACCGGCTCAGTGCCGATGGCTGCGGCTGCGAGGATGTGGATGAGTGCGCCTCCAGCCGTGGCGGCTGCGAGCACCACTGCACCAACCTGGCCGGCTCCTTCCAGTGCTCCTGCGAGGCCGGCTACCGGCTGCACGAGGACCGTAGGGGCTGCAGCGCCCTGGAGGAGCCGATGGTGGACCTGGACGGCGAGCTGCCTTTCGTGCGGCCCCTGCCCCACATTGCCGTGCTCCAGGACGAGCTGCCGCAACTCTTCCAGGATGACGACGTCGGGGCCGATGAGGAAGAGGCAGAGTTGCGGGGCGAACACACGCTCGAACGGAGGGACCTGCCTCCTGGGCCTGGATGGCTGTGATTGCCCCGAGGGCTGGACTGGGCTCATCTAGCATGAGAGTTGTCCTCCGGACACCTTTGGGAAGAACTGCAGCTTCTCCTGCAGCTGTCAGAATGGTGGGACCTGCGACTCTGTCACGGGGGCCTGCCGCTGCCCCCCGGGTGTCAGTGGAACTAACTGTGAGGATGGCTGCCCCAAGGGCTACTATGGCAAGCACTGTCGCAAGAAATGCAACTGTGCCAACCGGGGCCGGTGCCACCGCCTCTACGGGGCCTGCCTCTGCGACCCAGGGCTCTACGGCCGCTTCTGCCACCTCGCCTGCCCGCCGTGGGCCTTTGGGCCGGGCTGCTCGGAGGAGTGCCAGTGTGTGCAGCCCCACACGCAGTCCTGTGACAAGAGGGATGGCAGCTGCTCCTGCAAGGCTGGCTTCCGGGGCGAGCGCTGTCAGGCAGAGTGTGAGCCGGGCTACTTTGGGCCGGGGTGCTGGCAGGCATGCACCTGCCCAGTGGGCGTGGCCTGTGACTCCGTGAGCGGCGAGTGTGGGAAGCGGTGTCCTGCTGGCTTCCAGGGAGAGGACTGTGGCCAAGAGTGCCCGGTGGGGACCTTTGGCGTGAACTGCTCGAGCTCCTGCTCCTGTGGGGGGGCCCCCTGCCACGGGGTCACGGGGCAGTGCCGGTGTCCGCCGGGGAGGACTGGGGAAGACTGTGAGGCAGGTGAGTGTGAGGGCCTCTGGGGGCTGGGCTGCCAGGAGATCTGCCCAGCATGCCATAACGCTGCTCGCTGCGACCCTGAGACCGGAGCCTGCCTGTGCCTCCCTGGCTTTGTCGGCAGCCGCTGCCAGGACTGTGAGGCAGGCTGGTATGGTCCCAGCTGCCAGACAATGTGCTCTTGTGCCAATGATGGGCACTGCCACCAAGACACGGGACACTGCAGCTGTGCCCCCGGGTGGACCGGCTTTAGCTGCCAGAGAGCCTGTGATACTGGGCACTGGGGACCTGACTGCAGCCACCCCTGCAACTGCAGCGCTGGCCACGGGAGCTGTGATGCCATCAGCGGCCTGTGTCTGTGTGAGGCTGGCTACGTGGGCCCGCGGTGCGAGCAGTCAGAGTGTCCCCAGGGCCACTTTGGGCCCGGCTGTGAGCAGCGGTGCCAGTGTCAGCATGGAGCAGCCTGTGACCACGTCAGCGGGGCCTGCACCTGCCCGGCCGGCTGGAGGGGCACCTTCTGCGAGCATGCCTGCCCGGCCGGCTTCTTTGGATTGGACTGTCGCAGTGCCTGCAACTGCACCGCCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGAGTGCCTGCCCAGCCCACACCTACGGGCACAATTGCAGCCAGGCCTGTGCCTGCTTTAACGGGGCCTCCTGTGACCCTGTCCACGGGCAGTGCCACTGTGCCCCTGGCTGGATGGGGCCCTCCTGCCTGCAGGCCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCTGTGAGGTAGAGTGCCTCCCCCGGGACGTCAGAGCTGGCTGCCGGCACAGCGGCGGTTGCCTCAACGGGGGCCTGTGTGACCCGCACACGGGCCGCTGCCTCTGCCCAGCCGGCTGGACTGGGGACAAGTGTCAGAGCCCTGCAGCCTGTGCCAAGGGCACATTCGGGCCTCACTGTGAGGGGCGCTGTGCCTGCCGGTGGGGAGGCCCCTGCCACCTTGCCACCGGGGCCTGCCTCTGCCCTCCGGGGTGGCGGGGGCCTCATCTTTCTGCAGCCTGCCTGCGGGGCTGGTTTGGAGAGGCCTGTGCCCAGCGCTGCAGCTGCCCGCCTGGCGCTGCCTGCCACCACGTCACTGGGGCCTGCCGCTGTCCCCCTGGCTTCACTGGCTCCGGCTGCGAGCAGGCCTGCCCACCCGGCAGCTTTGGGGAGGACTGTGCGCAGATGTGCCAGTGTCCCGGTGAGAACCCGGCCTGCCACCCTGCCACCGGGACCTGCTCATGTGCTGCTGGCTACCACGGCCCCAGCTGCCAGCAACGATGTCCGCCCGGGCGGTATGGGCCAGGCTGTGAACAGCTGTGTGGGTGTCTCAACGGGGGCTCCTGTGATGCGGCCACGGGGGCCTGCCGCTGCCCCACTGGGTTCCTCGGGACGGACTGCAACCTCACCTGTCCGCAGGGCCGCTTCGGCCCCAACTGCACCCACGTGTGTGGGTGTGGGCAGGGGGCGGCCTGCGACCCTGTGACCGGCACCTGCCTCTGCCCCCCGGGGAGAGCCGGCGTCCGCTGTGAGCGAGGCTGCCCCCAGAACCGGTTTGGCGTGGGCTGCGAGCACACCTGCTCCTGCAGAAATGGGGGCCTGTGCCACGCCAGCAACGGCAGCTGCTCCTGTGGCCTGGGCTGGACGGGGCGGCACTGCGAGCTGGCCTGTCCCCCTGGGCGCTACGGAGCCGCCTGCCATCTGGAGTGCTCCTGCCACAACAACAGCACGTGTGAGCCTGCCACGGGCACCTGCCGCTGCGGCCCCGGCTTCTATGGCCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCTGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGGGTGTGAGCCAGGTTCATTTGGAGAGGGCTGCCACCAGCGCTGTGACTGTGACGGGGGGGCACCCTGTGACCCTGTCACCGGTCTCTGCCTTTGCCCACCAGGGCGCTCAGGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACGAATTCCCCGGGCTCGAGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCACCACCATCACCATTGAGTTTAATTCATTGATTTNOV37o, CG56449-11Protein SequenceSEQ ID NO: 8861547 aaMW at 161933.0kDSVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGCLSDVGECANANGGCAGRCRDTVGGFYCRWPPPSHQLQGDGETCQDVDECRTHNGGCQHRCVNTPGSYLCECKPGFRLHTDSRTCAINSCALGNGGCQHHCVQLTITRHRCQCRPGFQLQEDGRHCVRRSPCANRNGSCMHRCQVVRGLARCECHVGYQLAADGKACEDVDECAAGLAQCAHGCLNTQGSFKCVCHAGYELGADGRQCYRIEMEIVNSCEANNGGCSHGCSHTSAGPLCTCPRGYELDTDQRTCIRCRRLCRQPVLQQVCTNNPGGYECGCYAGYRLSADGCGCEDVDECASSRGGCEHHCTNLAGSFQCSCEAGYRLHEDRRGCSALEEPMVDLDGELPFVRPLPHIAVLQDELPQLFQDDDVGADEEEAELRGEHTLTEKFVCLDDSFGHDCSLTCDDCRNGGTCLLGLDGCDCPEGWTGLICNESCPPDTFGKNCSFSCSCQNGGTCDSVTGACRCPPGVSGTNCEDGCPKGYYGKHCRKKCNCANRGRCHRLYGACLCDPGLYGRFCHLACPPWAFGPGCSEECQCVQPHTQSCDKRDGSCSCKAGFRGERCQAECEPGYFGPGCWQACTCPVGVACDSVSGECGKRCPAGFQGEDCGQECPVGTFGVNCSSSCSCGGAPCHGVTGQCRCPPGRTGEDCEAGECEGLWGLGCQEICPACHNAARCDPETGACLCLPGFVGSRCQDCEAGWYGPSCQTMCSCANDGHCHQDTGHCSCAPGWTGFSCQHACDTGHWGPDCSHPCNCSAGHGSCDAISGLCLCEAGYVGPRCEQSECPQGHFGPGCEQRCQCQHGAACDHVSGACTCPAGWRGTFCEHACPAGFFGLDCRSACNCTAGAACDAVNGSCLCPAGRRGPRCAESACPAHTYGHNCSQACACFNGASCDPVHGQCHCAPGWMGPSCLQACPAGLYGDNCRHSCLCQNGGTCDPVSGHCACPEGWAGLACEVECLPRDVRAGCRHSGGCLNGGLCDPHTGRCLCPAGWTGDKCQSPAACAKGTFGPHCEGRCACRWGGPCHLATGACLCPPGWRGPHLSAACLRGWFGEACAQRCSCPPGAACHHVTGACRCPPGFTGSGCEQACPPGSFGEDCAQMCQCPGENPACHPATGTCSCAAGYHGPSCQQRCPPGRYGPGCEQLCGCLNGGSCDAATGACRCPTGFLGTDCNLTCPQGRFGPNCTHVCGCGQGAACDPVTGTCLCPPGRAGVRCERGCPQNRFGVGCEHTCSCRNGGLCHASNGSCSCGLGWTGRHCELACPPGRYGAACHLECSCHNNSTCEPATGTCRCGPGFYGQACEHPCPPGFHGAGCQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERGCEPGSFGEGCHQRCDCDGGAPCDPVTGLCLCPPGRSGATCNLDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREAGTLPASSRPTSRSGGPARHNOV37p,SNP13382514 ofSEQ ID NO: 887877 bpCG56449-04,ORF Start: ATG at 25ORF Stop: TAG at 535DNA SequenceSNP Pos: 430SNP Change: G to ACCGGAGCTGCCTGTGATGCCGTGAATGGCTCCTGCCTCTGCCCCGCTGGCCGCCGGGGCCCCCGCTGTGCCGAGACCTGCCCTGCCGGCCTGTACGGCGACAACTGTCGGCATTCCTGCCTCTGCCAGAACGGAGGGACCTGTGACCCTGTCTCAGGCCTGCGAGCACCCCTGTCCCCCTGGCTTCCACGGGGCTGGCCGCCAGGGGTTGTGCTGGTGTCAACATGGAGCCCCCTGCGACCCCATCAGTGGCCGATGCCTCTGCCCTGCCGGCTTCCACGGCCACTTCTGTGAGAGGGATTGCAGAAGGGGCCAGTTTGGGCCCAGCTGCACCCTGCACTGTGACTGCGGGGGTGGGGCTGACTGCGACCCTGTCAGTGGGCAGTGTCACTGTGTGGATGGCTACATGGGGCCCACGTGCCGGGAAGGTAGGCCCCTCCGGCTCCCCGAGAACCCGTCCTTAGCCCAGGGCTCAGCGGGCACACTGCCCGCCTCCAGCAGACCCACATCCCGGAGCGGTGGACCAGCGAGGCACTAGTAGAGGCAGTCCCGTGGAGCCCGCCTCTCCAGTCCCAGCCAGAGGGGACCCTGGCCTTTGGTGACCACTGAGAAGGACACTTCACGGGCCCAGAGCTCCTGGTACTGCCCTTCCTTTGAGGGCCGTGGAGGGCTGTGGACAGCCCAGCAACCTGTCGCTCTTGGAGGCTGGTGTGGCCTTGAGGAGGGAAGCCTCGCATGGCCGCTGGAAGAGAGGCGTCTCCTGGCCTGGCTCTGCAGAACCCAGGGGCACGCTCTGGGCCTGGGCTGAGGAAGTCCCGCTCTCCCGCGGCTCTGAGTTGGACTGAGGACAGGTGTGGGCGCCAGTGTGGGTGCAGGCGNOV37p,SNP13382514 ofCG56449-04,SEQ ID NO: 888MW at 17222.2kDProtein SequenceSNP Pos: 136170 aaSNP Change: Gly to ArgMAPASAPLAAGAPAVPRPALPACTATTVGIPASARTEGPVTLSQACEHPCPPGFHGAGRQGLCWCQHGAPCDPISGRCLCPAGFHGHFCERDCRRGQFGPSCTLHCDCGGGADCDPVSGQCHCVDGYMGPTCREGRPLRLPENPSLAQGSAGTLPASSRPTSRSGGPARH


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 37B.

TABLE 37BComparison of the NOV37 protein sequences.NOV37a------------------------------------------------------------NOV37b-----------------------------------------------MSFLEEARAAGRANOV37c------------------------------------------------------------NOV37d-------------------------------------------TGSTMSFLEEARAAGRANOV37eSEQYSLLPRAPPDIIADRLTDCSFPWVFSAVTVLDTKLSRRSSRGSTMSFLEEAPAAGRANOV37f--------------------------------------------HEALATMETDTLLLWVNOV37g-----------------------------------------------MPMGHSDRWSWRLNOV37h-----------------------------------------------MPMGHSDRWSWRLNOV37i-----------------------------------------------MPMGHSDRWSWRLNOV37j------------------------------------------------------------NOV37k-----------------------------------------------MPMGHSDRWSWRLNOV37l-----------------------------------------------MPMGHSDRWSWRLNOV37m-----------------------------------------------MSFLEEARAAGRANOV37n-----------------------------------------------MSFLEEARAAGPANOV37o------------------------------------------------------------NOV37a------------------------------------------------------------NOV37bVVLALVLLLLPAVPVGASVPPRPLLPLQPG-----------MPHVCAEQELTLVGRRQPCNOV37c------------------------------------------------------------NOV37dVVLALVLLLLPAVPVGASVPPRPLLPLQPG-----------MPHVCAEQELTLVGRRQPCNOV37eVVLALVLLLLPAVPVGASVPPRPLLPLQPG-----------MPHVCAEQELTLVGRRQPCNOV37fLLLWVPGSTGDAAQPARRARRTKLSRGSSVPPRPLLPLQPGMPHVCAEQELTLVGRRQPCNOV37gLRLALPLPVWLPAGGGRGADSPCLCS---------------RPHVCAEQELTLVGRRQPCNOV37hLRLALPLPVWLPAGGGRGADSPCLCS---------------RPHVCAEQELTLVGRRQPCNOV37iLRLALPLPVWLPAGGGRGADSPCLCS---------------RPHVCAEQELTLVGRRQPCNOV37j------------------------------------------------------------NOV37kLRLALPLPVWLPAGGGRGADSPCLCS---------------RPHVCAEQELTLVGRRQPCNOV37lLRLALPLPVWLPAGGGRGADSPCLCS---------------RPHVCAEQELTLVGRRQPCNOV37mVVLALVLLLLPAVPVGASVPPRPLLPLQPG-----------MPHVCAEQELTLVGRRQPCNOV37nVVLALVLLLLPAVPVGASVPPRPLLPLQPG-----------MPHVCAEQELTLVGRRQPCNOV37o---SVPPRPLLPLQPG-------------------------MPHVCAEQELTLVGRRQPCNOV37a------------------------------------------------------------NOV37bVQALSHTVPVWRAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGNOV37c------------------------------------------------------------NOV37dVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGNOV37eVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGNOV37fVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGNOV37gVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGNOV37hVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGNOV37iVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGNOV37j------------------------------------------------------------NOV37kVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWMQQPDEEGNOV37lVQALSHTVPVWKAGCGWQAWCVGHERRTVYYMGYRQVYTTEARTVLRCCRGWTQQPDEEGNOV37ni 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(SEQ ID NO: 858)NOV37b(SEQ ID NO: 860)NOV37c(SEQ ID NO: 862)NOV37d(SEQ ID NO: 864)NOV37e(SEQ ID NO: 866)NOV37f(SEQ ID NO: 868)NOV37g(SEQ ID NO: 870)NOV37h(SEQ ID NO: 872)NOV37i(SEQ ID NO: 874)NOV37j(SEQ ID NO: 876)NOV37k(SEQ ID NO: 878)NOV37l(SEQ ID NO: 880)NOV37m(SEQ ID NO: 882)NOV37n(SEQ ID NO: 884)NOV37o(SEQ ID NO: 886)


Further analysis of the NOV37a protein yielded the following properties shown in Table 37C.

TABLE 37CProtein Sequence Properties NOV37aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 16; peak value 6.60PSG score:2.20GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −5.33possible cleavage site: between 14 and 15>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 5.83 (at 18)ALOM score: 5.83 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 6Charge difference: 0.0 C(1.0)-N(1.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):1.11Hyd Moment(95):2.89G content:2D/E content:1S/T content:6Score: −3.38Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 45 ART|EGNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 7.1%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):65.2%: nuclear21.7%: mitochondrial 8.7%: cytoplasmic 4.3%: extracellular, including cell wall>> prediction for CG56449-04 is nuc (k = 23)


A search of the NOV37a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 37D.

TABLE 37DGeneseq Results for NOV37aNOV37aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAY72716HMEIR04 clone human attractin-like38 . . . 170126/176(71%)6e−73protein - Homo sapiens, 310 aa.135 . . . 310 126/176(71%)[WO200116156-A1, 08-MAR-2001]AAY72715HFICU08 clone human attractin-like38 . . . 170107/133(80%)4e−65protein - Homo sapiens, 474 aa.342 . . . 474 108/133(80%)[WO200116156-A1, 08-MAR-2001]AAY72740HMEIR04 clone human attractin-like55 . . . 170113/159(71%)1e−63partial protein - Homo sapiens, 159 aa. 1 . . . 159113/159(71%)[WO200116156-A1, 08-MAR-2001 ]ABB72269Murine protein isolated from skin cells38 . . . 13474/140(52%)4e−40SEQ ID NO: 458 - Mus sp, 296 aa.135 . . . 274 79/140(55%)[WO200190357-A1, 29-NOV-2001]ABB72216Murine protein isolated from skin cells38 . . . 13474/140(52%)4e−40SEQ ID NO: 332 - Mus sp, 299 aa.138 . . . 277 79/140(55%)[WO200190357-A1, 29-NOV-2001]


In a BLAST search of public sequence databases, the NOV37a protein was found to have homology to the proteins shown in the BLASTP data in Table 37E.

TABLE 37EPublic BLASTP Results for NOV37aNOV37aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueO75095MEGF6 - Homo sapiens (Human),46 . . . 17099/134(73%)5e−571246 aa (fragment).1113 . . . 1246 105/134(77%)O88281MEGF6 - Rattus norvegicus (Rat),38 . . . 16482/170(48%)1e−411574 aa.1413 . . . 1571 93/170(54%)AAH39980Hypothetical protein - Mus musculus38 . . . 13474/140(52%)1e−39(Mouse), 546 aa.385 . . . 524 79/140(55%)Q8BKK7MEGF11 protein - Mus musculus46 . . . 13745/92(48%)7e−23(Mouse), 947 aa.183 . . . 274 54/92(57%)Q96KG6MEGF11 protein (Hypothetical46 . . . 13745/92(48%)7e−23protein KIAA1781) - Homo sapiens140 . . . 231 54/92(57%)(Human), 969 aa.


PFam analysis predicts that the NOV37a protein contains the domains shown in the Table 37F.

TABLE 37FDomain Analysis of NOV37aIdentities/SimilaritiesNOV37a Matchfor theExpectPfam DomainRegionMatched RegionValuelaminin_EGF63 . . . 10217/60 (28%)0.3730/60 (50%)EGF102 . . . 132 11/47 (23%)0.1623/47 (49%)


Example 38

The NOV38 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 38A.

TABLE 38ANOV38 Sequence AnalysisNOV38a, CG56594-01SEQ ID NO: 889695 bpDNA SequenceORF Start: ATG at 53ORF Stop: TGA at 662GCACCCTGGCCCAGCTCTGAGTCCTGGGACCCTCGGTCCTCTCTCCTGGGCCATGGCCAACTCAGGCCTCCAGCTCCTGGGCTACTTCTTGGCCCTGGGTGGCTGGGTGGGCATCATTGCTAGCACAGCCCTGCCACAGTGGAAGCAGTCTTCCTACGCAGGCGACGCCATCATCACTGCCGTGGGCCTCTATGAAGGGCTCTGGATGTCCTGCGCCTCCCAGAGCACTGGGCAAGTGCAGTGCAAGCTCTACGACTCGCTGCTCGCCCTGGACGGTAGGCCCCAGGCCGCGCGGGCCCTGATGGTGGTGGCCGTGCTCCTGGGCTTCGTGGCCATGGTCCTCAGCGTAGTTGGCATGAAGTGTACGCGGGTGGGAGACAGCAACCCCATTGCCAAGGGCCGTGTTGCCCCTGGTGACCCAGGAGTTCTTCAACCCAGAATTTGGCCCAGCCCTGTTCGTGGGCTGGGCCTCAGCTGGCCTGGCCGTGCTGGGCGGCTCCTTCCTCTGCTGCACATGCCCGGAGCCAGAGAGACCCAACAGCAGCCCACAGCCCTATCGGCCTGGACCCTCTGCTGCTGCCCGAGAGTACGTCTGAGCTCCGCCTGCCCTGGCCAGCCCCCCACCCANOV38a, CG56594-01Protein SequenceSEQ ID NO: 890203 aaMW at 21174.4kDMANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGRPQAARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPEFGPALFVGWASAGLAVLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYVNOV38b, 277685552SEQ ID NO: 891655 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGGCCAACTCAGGCCTCCAGCTCCTGGGCTACTTCTTGGCCCTGGGTGGCTGGGTGGGCATCATTGCTAGCACAGCCCTGCCACAGTGGAAGCAGTCTTCCTACGCAGGCGACGCCATCATCACTGCCGTGGGCCTCTATGAAGGGCTCTGGATGTCCTGCGCCTCCCAGAGCACTGGGCAAGTGCAGTGCAAGCTCTACGACTCGCTGCTCGCCCTGGACGGTCACATCCAATCAGCGCGGGCCCTGATGGTGGTGGCCGTGCTCCTGGGCTTCGTGGCCATGGTCCTCAGCGTAGTTGGCATGAAGTGTACGCGGGTGGGAGACAGCAACCCCATTGCCAAGGGCCGTGTTGCCATCGCCGGGGGAGCCCTCTTCATCCTGGCAGGCCTCTGCACTTTGACTGCTGTCTCGTGGTATGCCACCCTGGTGACCCAGGAGTTCTTCAACCCAAGCACACCTGTCAATGCCAGGTATGAATTTGGCCCAGCCCTGTTCGTGGGCTGGGCCTCAGCTGGCCTGGCCGTGCTGGGCGGCTCCTTCCTCTGCTGCACATGCCCGGAGCCAGAGAGACCCAACAGCAGCCCACAGCCCTATCGGCCTGGACCCTCTGCTGCTGCCCGAGAGTACGTCGTCGACGGCNOV38b, 277685552Protein SequenceSEQ ID NO: 892218 aaMW at 22694.0kDTGSTMANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYVVDGNOV38c, 277685649SEQ ID NO: 893583 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCAGCACAGCCCTGCCACAGTGGAAGCAGTCTTCCTACGCAGGCGACGCCATCATCACTGCCGTGGGCCTCTATGAAGGGCTCTGGATGTCCTGCGCCTCCCAGAGCACTGGGCAAGTGCAGTGCAAGCTCTACGACTCGCTGCTCGCCCTGGACGGTCACATCCAATCAGCGCGGGCCCTGATGGTGGTGGCCGTGCTCCTGGGCTTCGTGGCCATGGTCCTCAGCGTAGTTGGCATGAAGTGTACGCGGGTGGGAGACAGCAACCCCATTGCCAAGGGCCGTGTTGCCATCGCCGGGGGAGCCCTCTTCATCCTGGCAGGCCTCTGCACTTTGACTGCTGTCTCGTGGTATGCCACCCTGGTGACCCAGGAGTTCTTCAACCCAAGCACACCTGTCAATGCCAGGTATGAATTTGGCCCAGCCCTGTTCGTGGGCTGGGCCTCAGCTGGCCTGGCCGTGCTGGGCGGCTCCTTCCTCTGCTGCACATGCCCGGAGCCAGAGAGACCCAACAGCAGCCCACAGCCCTATCGGCCTGGACCCTCTGCTGCTGCCCGAGAGTACGTCGTCGACGGCNOV38c, 277685649Protein SequenceSEQ ID NO: 894194 aaMW at 20246.1kDTGSSTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYVVDGNOV38d, 277685616SEQ ID NO: 895556 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCTCAGGCCTCCAGCTCCTGGGCTACTTCTTGGCCCTGGGTGGCTGGGTGGGCATCATTGCTAGCACAGCCCTGCCACAGTGGAAGCAGTCTTCCTACGCAGGCGACGCCATCATCACTGCCGTGGGCCTCTATGAAGGGCTCTGGATGTCCTGCGCCTCCCAGAGCACTGGGCAAGTGCAGTGCAAGCTCTACGACTCGCTGCTCGCCCTGGACGGTCACATCCAATCAGCGCGGGCCCTGATGGTGGTGGCCGTGCTCCTGGGCTTCGTGGCCATGGTCCTCAGCGTAGTTGGCATGAAGTGTACGCGGGTGGGAGACAGCAACCCCATTGCCAAGGGCCGTGTTGCCATCGCCGGGGGAGCCCTCTTCATCCTGGCAGGCCTCTGCACTTTGACTGCTGTCTCGTGGTATGCCACCCTGGTGACCCAGGAGTTCTTCAACCCAAGCACACCTGTCAATGCCAGGTATGAATTTGGCCCAGCCCTGTTCGTGGGCTGGGCCTCAGCTGGCCTGGCCGTGCTGGGCGGCTCCTTCCTCGTCGACGGCNOV38d, 277685616Protein SequenceSEQ ID NO: 896185 aaMW at 19131.1kDTGSSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVLGGSFLVDG


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 38B.

TABLE 38BComparison of the NOV38 protein sequences.NOV38a----MANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASNOV38bTGSTMANSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASNOV38c------------------------TGSSTALPQWKQSSYAGDAIITAVGLYEGLWMSCASNOV38d----TGSSGLQLLGYFLALGGWVGIIASTALPQWKQSSYAGDAIITAVGLYEGLWMSCASNOV38aQSTGQVQCKLYDSLLALDGRPQAARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGNOV38bQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGNOV38cQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGNOV38dQSTGQVQCKLYDSLLALDGHIQSARALMVVAVLLGFVAMVLSVVGMKCTRVGDSNPIAKGNOV38aRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNP--------EFGPALFVGWASAGLAVNOV38bRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVNOV38cRVAIAGGALFILGGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVNOV38dRVAIAGGALFILAGLCTLTAVSWYATLVTQEFFNPSTPVNARYEFGPALFVGWASAGLAVNOV38aLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYV---NOV38bLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYVVDGNOV38cLGGSFLCCTCPEPERPNSSPQPYRPGPSAAAREYVVDGNOV38dLGGSFLVDG-----------------------------NOV38a(SEQ ID NO: 890)NOV38b(SEQ ID NO: 892)NOV38c(SEQ ID NO: 894)NOV38d(SEQ ID NO: 896)


Further analysis of the NOV38a protein yielded the following properties shown in Table 38C.

TABLE 38CProtein Sequence Properties NOV38aSignalP analysis:Cleavage site between residues 24 and 25PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 30; peak value 9.10PSG score:4.70GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −4.10possible cleavage site: between 17 and 18>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 4INTEGRALLikelihood =Transmembrane 8-24−1.75INTEGRALLikelihood =Transmembrane 84-100−12.15INTEGRALLikelihood =Transmembrane 118-134 −6.42INTEGRALLikelihood =Transmembrane 153-169 −0.64PERIPHERALLikelihood = 2.28 (at 39)ALOM score: −12.15 (number of TMSs: 4)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 15Charge difference: −1.0 C(0.0)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):4.90Hyd Moment(95):7.23G content:6D/E content:1S/T content:5Score: −6.38Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 5.4%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):66.7%: endoplasmic reticulum33.3%: mitochondrial>> prediction for CG56594-01 is end (k = 9)


A search of the NOV38a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 38D.

TABLE 38DGeneseq Results for NOV38aNOV38aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABG27980Novel human diagnostic protein1 . . . 203200/211 (94%) e−111#27971 - Homo sapiens, 254 aa.44 . . . 254 201/211 (94%)[WO200175067-A2, 11-OCT-2001 ]AAO20545Human Claudin-19 protein - Homo1 . . . 203197/211 (93%) e−109sapiens, 211 aa. [WO200214499-A2,1 . . . 211198/211 (93%)21-FEB-2002]AAO20546Murine Claudin-19 protein (partial11 . . . 195 172/193 (89%)1e−94sequence) - Mus sp, 193 aa.1 . . . 193175/193 (90%)[WO200214499-A2, 21-FEB-2002]AAU96166Human secreted protein, SEQ ID No84 . . . 203 120/128 (93%)2e−6368 - Homo sapiens, 128 aa.1 . . . 128120/128 (93%)[WO200224721-A1, 28-MAR-2002]ABG64826Human albumin fusion protein #1501 -84 . . . 203 120/128 (93%)2e−63Homo sapiens, 128 aa.1 . . . 128120/128 (93%)[WO200177137-A1, 18-OCT-2001]


In a BLAST search of public sequence databases, the NOV38a protein was found to have homology to the proteins shown in the BLASTP data in Table 38E.

TABLE 38EPublic BLASTP Results for NOV38aNOV38aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8N6F1Claudin-19 - Homo sapiens (Human),1 . . . 203200/211 (94%) e−111211 aa.1 . . . 211201/211 (94%)Q9ET38Claudin-19 - Mus musculus (Mouse),1 . . . 203191/211 (90%) e−105211 aa.1 . . . 211195/211 (91%)O95832Claudin-1 (Senescence-associated1 . . . 203115/212 (54%)1e−62epithelial membrane protein) - Homo1 . . . 211151/212 (70%)sapiens (Human), 211 aa.AAP35475Claudin 7 - Homo sapiens (Human),1 . . . 203112/211 (53%)3e−62211 aa.1 . . . 211149/211 (70%)Q8AVG4Similar to claudin 1 - Xenopus laevis1 . . . 203112/211 (53%)6e−62(African clawed frog), 211 aa.1 . . . 211142/211 (67%)


PFam analysis predicts that the NOV38a protein contains the domains shown in the Table 38F.

TABLE 38FDomain Analysis of NOV38aIdentities/SimilaritiesNOV38a Matchfor theExpectPfam DomainRegionMatched RegionValuePMP22_Claudin4 . . . 17461/188(32%)1.7e−56142/188(76%)


Example 39

The NOV39 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 39A.

TABLE 39ANOV39 Sequence AnalysisNOV39a, CG56653-08SEQ ID NO: 8971000 bpDNA SequenceORF Start: ATG at 2ORF Stop: TAG at 980CATGGAGCTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAGGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACAATATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCGAGCCTCAATGGTCTCTACCTCATGGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGACGGGCCAGGACCCCTAANOV39a, CG56653-08Protein SequenceSEQ ID NO: 898326 aaMW at 35064.1kDMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHNIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASSLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39b, 214374274SEQ ID NO: 899651 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAACCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39b, 214374274Protein SequenceSEQ ID NO: 900217 bpMW at 24272.7kDGSGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNIIQFAKYKSFKVADEAEKYKLVLGTFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPALENOV39c, 214374256SEQ ID NO: 901651 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAATGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39c, 214374256Protein SequenceSEQ ID NO: 902217 aaMW at 24242.6kDGSGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRPMDGSVDFYRDWAAYKQGFLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPALENOV39d, 214374252SEQ ID NO: 903651 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGGTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39d, 214374252Protein SequenceSEQ ID NO: 904217 aaMW at 24200.6kDGSGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTGFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPALENOV39e, 214458492SEQ ID NO: 905651 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGTTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCGGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39e, 214458492Protein SequenceSEQ ID NO: 906217 aaMW at 24270.7kDGSRLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPALENOV39f, 214458488SEQ ID NO: 907651 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAATGACGACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39f, 214458488Protein SequenceSEQ ID NO: 908217 aaMW at 24243.6kDGSGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDDIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPALENOV39g, 214374236SEQ ID NO: 909651 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGAGGGCACTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39g 214374236Protein SequenceSEQ ID NO: 910217 aaMW at 24216.6kDGSGPRNCKDLLDRGHFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPALENOV39h, CG56653-01SEQ ID NO: 9111187 bpDNA SequenceORF Start: ATG at 16ORF Stop: TAG at 973CTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGACGGGCCAGGACCCCTCCACATGCACCTGCTAGTGGGGAGGCCACACCCACAAGCGCTGCGTCGTGGAAGTCACCCCATTTCCCCAGCCAGACACACTCCCATGACGCCCACAGCTGCCCCTTTGCCCCCAGCTCAGTCAAGCCGCCACATGCCCACAACCTCACCAGAGGGAGAATTATGTTTCTAAATATGTTTACTTTGGGACAGAAAANOV39h, CG56653-01Protein SequenceSEQ ID NO: 912319 aaMW at 34388.3kDMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAOGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39i, CG56653-02SEQ ID NO: 9131332 bpDNA SequenceORF Start: ATG at 183ORF Stop: TAG at 1107TTTTAGGTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGAGTTGAGAAACTGTGGCACAAGGCGAGAGCTGGTTTCCTCTGCCCTGTTAGAGCTGGGGGACTCTTCAGAGTCAAAGGCCAGAGAGCATGGAGCTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGGCAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAATGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGACGGGCCAGGACCCCTCCACATGCACCTGCTAGTGGGGAGGCCACACCCACAAGCGCTGCGTCGTGGAAGTCACCCCATTTCCCCAGCCAGACACACTCCCATGACGCCCACAGCTCTAATATGTTTACTTTTGGGGACAGAAAAAAAAAAAAANOV39i, CG56653-02Protein SequenceSEQ ID NO: 914308 aaMW at 33393.2kDMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSGKLTILRGCPGLPGAPGPKGEAGVIGERGDRGEKGNRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRNDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39j, CG56653-03SEQ ID NO: 915728 bpDNA SequenceORF Start: at 1ORF Stop: TAG at 574CTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAGGAGGCCACACCCACAAGCGCTGCGTCGTGGAAGTCACCCCATTTCCCCAGCCAGACACACTCCCATGACGCCCACAGCTGCCCCTTTGCCCCCAGCTCAGTCAAGCCGCCACATGNOV39j, CG56653-03Protein SequenceSEQ ID NO: 916191 aaMW at 20220.7kDLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39k, CG56653-04SEQ ID NO: 9171104 bpDNA SequenceORF Start: ATG at 16ORF Stop: TAG at 883CTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCACGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGACCAAAGGGAGAGGCAGGTGTCATTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGAGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTTACTGTGCTCTGTGACATGGATACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAATGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAAAGTCACCCCATTTCCCCAGCCAGACACACTCCCATGACGCCCACAGCTGCCCCTTTGCCCCCAGCTCAGTCAAGCCGCCACATGCCCACAACCTCACCAGAGGGAGAATTATGTTTCTAAATATGTTTACTTTGGGACAGAAAAAAAAAAANOV39k, CG56653-04Protein SequenceSEQ ID NO: 918289 aaMW at 31404.9kDMARGLAVLLVLFLHITNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGEKGDAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39l, CG56653-05SEQ ID NO: 919900 bpDNA SequenceORF Start: at 7ORF Stop: at 895GGATCCGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGGGCCCGCCCTCGAGNOV39l, CG56653-05Protein SequenceSEQ ID NO: 920296 aaMW at 31819.1kDADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKGPANOV39m, CG56653-06SEQ ID NO: 921988 bpDNA SequenceORF Start: ATG at 56ORF Stop: TAG at 905GTTTCCTCTGCCCTGTTAGAGCTGGGGGACTCTTCAGAGTCAAAGGCCAGAGAGCATGGAGCTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGTCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGCAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGACGGGCCAGGACCCCTCCACATGCACCTGCTAGTGGGGAGGCCACACCCACAAGCGCTGCGTCGTGGAAGTCACCCATTTCNOV39m, CG56653-06Protein SequenceSEQ ID NO: 922283 aaMW at 30136.7kDMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINCSAAKGYKYSYKVSEMKVRPANOV39n, CG56653-07SEQ ID NO: 923970 bpDNA SequenceORF Start: at 2ORF Stop: TAG at 950GGGGCTCGCTGTCATGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAATGACAACATCCACGCCTTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGACGGGCCAGGACCCCTCCNOV39n, CG56653-07Protein SequenceSEQ ID NO: 924316 aaMW at 34047.9kDGLAVMLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTTLRGCPGLPGAPGPKGEAGVIGERGERGLPDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39o, CG56653-09SEQ ID NO: 9251144 bpDNA SequenceORF Start: ATG at 183ORF Stop: TAG at 981TTTTAGGTCTGTTTGTCGTAGGCAGATGGAGCTTGTTATAATTATGCCTCATAGGGATAGTACAAGGAGCCCTGTTAGAGCTGGGGGACTCTTCAGAGTCAAAGGCCAGAGAGCATGGAGCTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAQACGGGCCAGGACCCCTCCACATGCACCTGCTAGTGGGGAGGCCACACCCACAAGCGCTGCGTCGTGGAAGTCACCCCATTTCCCCAGCCAGACACACTCCCATGACGCCCACAGCTGCCCCTTTGCCCCCAGCTCAGTCAAGCCGCCACATGCCCACAACCNOV39o, CG56653-09Protein SequenceSEQ ID NO: 926266 aaMW at 28476.8kDMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39p, CG56653-10SEQ ID NO: 927651 bpDNA SequenceORF Start: at 7ORF Stop: at 646GGATCCGGCCCACGCAACTGCAAGGACCTGCTAGACCGAGGGCACTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAAGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39p, CG56653-10Protein SequenceSEQ ID NO: 928213 aaMW at 23830.2kDGPRNCKDLLDRGHFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39q, CG56653-11SEQ ID NO: 9291045 bpDNA SequenceORF Start: ATG at 38ORF Stop: TAG at 1016GAGCTGGGGGACTCTTCAGAGTCAAAGGCCAGAGAGCATGGAGCTGAGTGGAGCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCTAGACGGGCCAGGACCCCTCCACATGCACCNOV39q, CG56653-11Protein SequenceSEQ ID NO: 930326 aaMW at 35078.1kDMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPANOV39r, CG56653-12SEQ ID NO: 931972 bpDNA SequenceORF Start: ATG at 10ORF Stop: at 967GGATCCACCATGGCCCGGGGGCTCGCTGTCCTGCTAGTCTTGTTCCTGCATATCAAGAACCTGCCTGCCCAGGCTGCGGACACATGTCCAGAGGTGAAGGTGGTGGGCCTGGAGGGCTCTGACAAGCTCACCATTCTCCGAGGCTGCCCGGGGCTGCCCGGGGCCCCAGGGCCAAAGGGAGAGGCAGGTGTCATTGGAGAGAGAGGAGAACGCGGTCTCCCTGGAGCCCCTGGAAAGGCAGGACCAGTGGGGCCCAAAGGAGACCGAGGAGAGAAGGGGATGCGTGGAGAGAAAGGAGACGCTGGGCAGTCTCAGTCGTGTGCGACAGGCCCACGCAACTGCAAGGACCTGCTAGACCGGGGGTATTTCCTGAGCGGCTGGCACACCATCTACCTGCCCGACTGCCGGCCCCTGACTGTGCTCTGTGACATGGACACGGACGGAGGGGGCTGGACCGTTTTCCAGCGGAGGATGGATGGCTCTGTGGACTTCTATCGGGACTGGGCCGCATACAAGCAGGGCTTCGGCAGTCAGCTGGGGGAGTTCTGGCTGGGGAACGACAACATCCACGCCCTGACTGCCCAGGGAAGCAGCGAGCTCCGTGTAGACCTGGTGGACTTTGAGGGCAACCACCAGTTTGCTAAGTACAAATCATTCAAGGTGGCTGACGAGGCAGAGAAGTACAAGCTGGTACTGGGAGCCTTTGTCGGGGGCAGTGCGGGTAATTCTCTAACGGGCCACAACAACAACTTCTTCTCCACCAAAGACCAAGACAATGATGTGAGTTCTTCGAATTGTGCTGAGAAGTTCCAGGGAGCCTGGTGGTACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACCCCATGAGAGCTATGCCAATGGTATCAACTGGAGTGCGGCGAAGGGGTACAAATATAGCTACAAGGTGTCAGAGATGAAGGTGCGGCCCGCCCTCGAGNOV39r, CG56653-12Protein SequenceSEQ ID NO: 932319 aaMW at 34388.3kDMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYANGINWSAAKGYKYSYKVSEMKVRPA


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 39B.

TABLE 39BComparison of the NOV39 protein sequences.NOV39aMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39b------------------------------------------------------------NOV39c------------------------------------------------------------NOV39d------------------------------------------------------------NOV39e------------------------------------------------------------NOV39f------------------------------------------------------------NOV39g------------------------------------------------------------NOV39h-------MARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39iMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSGKLTILRGCPGLPGAPNOV39j-------------------LHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39k-------MARGLAVLLVLFLHITNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39l-----------------------------ADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39mMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39n----------GLAVMLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTTLRGCPGLPGAPNOV39oMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39p------------------------------------------------------------NOV39qMELSGATMARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39r-------MARGLAVLLVLFLHIKNLPAQAADTCPEVKVVGLEGSDKLTILRGCPGLPGAPNOV39aGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39b---------------------------------------------------GSGPRNCKDNOV39c---------------------------------------------------GSGPRNCKDNOV39d---------------------------------------------------GSGPRNCKDNOV39e---------------------------------------------------GSGPRNCKDNOV39f---------------------------------------------------GSGPRNCKDNOV39g---------------------------------------------------GSGPRNCKDNOV39hGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39iGPKGEAGVIGERG------------------DRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39jGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39kGPKGEAGVIGE------------------------------KGDAGQSQSCATGPRNCKDNOV39lGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39mGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39nGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39oGPKGEAGVIGERGERGLPGAPGKAGPVGPKGDRGEKGMRGEKGDAGQSQSCATGPRNCKDNOV39p-----------------------------------------------------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---------------------NOV39kLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGNOV39lLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGNOV39mLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFEGNHQFAKYKSFNOV39nLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFYRDWAAYKQGFGNOV39oLLDRGYFLSGWHTIYLPDCRPLTVLCDMDTDGGGWTVFQRRMDGSVDFY-----------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------------------------------------------------------------NOV39kSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVNOV39lSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVNOV39mKVADEAEKYKL------------------------------------V-------LGAFVNOV39nSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVNOV39o------RDWAA------------------------------------Y-------KQGFGNOV39pSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVNOV39qSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVNOV39rSQLGEFWLGNDNIHALTAQGSSELRVDLVDFEGNHQFAKYKSFKVADEAEKYKLVLGAFVNOV39aGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASSLNGLYLMGPHESYNOV39bGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39cGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39dGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39eGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39fGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39gGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39hGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39iGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39j----------------------------------GAWWYADCHASNLNGLYLMGPHESYNOV39kGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39lGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39mGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39nGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39oSQLGGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39pGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39qGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39rGGSAGNSLTGHNNNFFSTKDQDNDVSSSNCAEKFQGAWWYADCHASNLNGLYLMGPHESYNOV39aANGINWSAAKGYKYSYKVSEMKVRPA--NOV39bANGINWSAAKGYKYSYKVSEMKVRPALENOV39cANGINWSAAKGYKYSYKVSEMKVRPALENOV39dANGINWSAAKGYKYSYKVSEMKVRPALENOV39eANGINWSAAKGYKYSYKVSEMKVRPALENOV39fANGINWSAAKGYKYSYKVSEMKVRPALENOV39gANGINWSAAKGYKYSYKVSEMKVRPALENOV39hANGINWSAAKGYKYSYKVSEMKVRPA--NOV39iANGINWSAAKGYKYSYKVSEMKVRPA--NOV39jANGINWSAAKGYKYSYKVSEMKVRPA--NOV39kANGINWSAAKGYKYSYKVSEMKVRPA--NOV39lANGINWSAAKGYKYSYKVSEMKGPA---NOV39mANGINCSAAKGYKYSYKVSEMKVRPA--NOV39nANGINWSAAKGYKYSYKVSEMKVRPA--NOV39oANGINWSAAKGYKYSYKVSEMKVRPA--NOV39pANGINWSAAKGYKYSYKVSEMKVRPA--NOV39qANGINWSAAKGYKYSYKVSEMKVRPA--NOV39rANGINWSAAKGYKYSYKVSEMKVRPA--NOV39a(SEQ ID NO: 898)NOV39b(SEQ ID NO: 900)NOV39c(SEQ ID NO: 902)NOV39d(SEQ ID NO: 904)NOV39e(SEQ ID NO: 906)NOV39f(SEQ ID NO: 908)NOV39g(SEQ ID NO: 910)NOV39h(SEQ ID NO: 912)NOV39i(SEQ ID NO: 914)NOV39j(SEQ ID NO: 916)NOV39k(SEQ ID NO: 918)NOV39l(SEQ ID NO: 920)NOV39m(SEQ ID NO: 922)NOV39n(SEQ ID NO: 924)NOV39o(SEQ ID NO: 926)NOV39p(SEQ ID NO: 928)NOV39q(SEQ ID NO: 930)NOV39r(SEQ ID NO: 932)


Further analysis of the NOV39a protein yielded the following properties shown in Table 39C.

TABLE 39CProtein Sequence Properties NOV39aSignalP analysis:Cleavage site between residues 30 and 31PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 1; neg. chg 1H-region:length 12; peak value 11.35PSG score:6.95GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −3.79possible cleavage site: between 29 and 30>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 6-22−3.35PERIPHERALLikelihood = 4.51 (at 234)ALOM score: −3.35 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 13Charge difference: −1.5 C(−0.5)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 6)MITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):3.15Hyd Moment(95):5.83G content:2D/E content:2S/T content:2Score: −6.93Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 20 ARG|LANUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 10.7%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:KKXX-like motif in the C-terminus: KVRPSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: mitochondrial30.4%: cytoplasmic 8.7%: Golgi 8.7%: endoplasmic reticulum 4.3%: vacuolar 4.3%: extracellular, including cell wall 4.3%: nuclear 4.3%: vesicles of secretory system>> prediction for CG56653-08 is mit (k = 23)


A search of the NOV39a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 39D.

TABLE 39DGeneseq Results for NOV39aNOV39aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAR94183Human 35 kDa opsonin protein P35 -1 . . . 326248/326 (76%)e−146Homo sapiens, 313 aa. [JP08038182-A,1 . . . 313266/326 (81%)13-FEB-1996]AAR30971TGF-beta-1 binding protein - Sus1 . . . 324239/324 (73%)e−145scrofa, 324 aa. [WO9222319-A1,2 . . . 322269/324 (82%)23-DEC-1992]AAR94179Human 35 kDa opsonin protein P3528 . . . 326 235/299 (78%)e−140fragment (III) residues 26-313 - Homo2 . . . 288252/299 (83%)sapiens, 288 aa. [JP08038182-A,13-FEB-1996]AAR94178Human 35 kDa opsonin protein P35109 . . . 326 180/218 (82%)e−110fragment (II) residues 96-313 - Homo1 . . . 218194/218 (88%)sapiens, 218 aa. [JP08038182-A,13-FEB-1996]AAB29658Human membrane-associated protein25 . . . 324 143/300 (47%)1e−77 HUMAP-15 - Homo sapiens, 299 aa.21 . . . 299 189/300 (62%)[WO200065054-A2, 02-NOV-2000]


In a BLAST search of public sequence databases, the NOV39a protein was found to have homology to the proteins shown in the BLASTP data in Table 39E.

TABLE 39EPublic BLASTP Results for NOV39aNOV39aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueS61517ficolin-1 precursor - human, 326 aa.1 . . . 326326/326(100%)0.01 . . . 326326/326(100%)O00602Ficolin 1 precursor (Collagen/fibrinogen1 . . . 326324/326(99%)0.0domain-containing protein 1) (Ficolin-A)1 . . . 326325/326(99%)(Ficolin A) (M-Ficolin) - Homo sapiens(Human), 326 aa.Q29042Ficolin - Sus scrofa (Pig), 326 aa.1 . . . 324251/324(77%)e−1531 . . . 324278/324(85%)P57756Ficolin 2 precursor (Collagen/fibrinogen8 . . . 324242/317(76%)e−150domain-containing protein 2) (Ficolin-B)1 . . . 317270/317(84%)(Ficolin B) (Serum lectin p35) (EBP-37)(Hucolin) - Rattus norvegicus (Rat), 319aa.Q29041Ficolin - Sus scrofa (Pig), 323 aa.1 . . . 324240/324(74%)e−1441 . . . 321269/324(82%)


PFam analysis predicts that the NOV39a protein contains the domains shown in the Table 39F.

TABLE 39FDomain Analysis of NOV39aIdentities/SimilaritiesNOV39a Matchfor theExpectPfam DomainRegionMatched RegionValueCollagen50 . . . 10827/60(45%)3.1e−0946/60(77%)fibrinogen_C114 . . . 326 116/272(43%)2.2e−91159/272(58%)


Example 40

The NOV40 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 40A.

TABLE 40ANOV40 Sequence AnalysisNOV40a, CG56806-01SEQ ID NO: 9331500 bpDNA SequenceORF Start: ATG at 74ORF Stop: TGA at 1490CTTCCGAGCGGGCGCCCGTCCGCCCTGCCGCCGCCGCCGCCGCCGCTTCGCCTGCCGGCCTGAGAGCGGGACCATGGATGAAAGGTTCAACAAGTGGCTGCTGACGCCGGTGCTCACTCTCCTCTTCGTGGTCATCATGTACCAGTACGTGTCCCCCTCCTGCACCAGCTCCTGCACCAACTTCGGGGAGCAGCCCCGCGCGGGGGAGGCCGGCCCGCCCGCCGTCCCGGGTCCCGCCCGCCGGGCTCAGGCGCCGCCGGAGGAGTGGGAGCAGAGGAGGCCCCAGTTGCCCCCGCCGCCCCGGGGGCCCCCCGAGGGACCTCGGGGGGCCGCGGCGCCGGAGGAGGAGGACGAGGAGCCCGGAGACCCCCGGGAGGGGGAGGAAGAGGAGGAGGAAGACGAGCCGGACCCCGAGGCCCCGGAAAACGGCTCCCTGCCCCGATTCGTGCCGCGCTTCAACTTCAGCCTGAAGGACCTGACCCGCTTCGTGGATTTCAACATCAAAGGGCGCGACGTGATCGTGTTCCTCCACATCCAGAAGACGGGGGGCACCACTTTCGGCCGGCACCTGGTGAAGAACATCCGGCTGGAGCAGCCTTGTAGCTGCAAAGCCCGGCTGGAGCTGCGGGCTGCACGCCGACTGGACGGAGCTCACCAACTGCGTGCCGGCCATCATGGAGAAGAAGGACTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGCGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGTGACCTCCTGCNOV40a, CG56806-01Protein SequenceSEQ ID NO: 934472 aaMW at 54971.3kDMDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARRAQAPPEEWEQRRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWNOV40b, 248061366SEQ ID NO: 935750 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGCGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGGGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGCTCGAGGCGNOV40b, 248061366Protein SequenceSEQ ID NO: 936250 aaMW at 29889.3kDRGTCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDGLPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40c, 246837961SEQ ID NO: 937750 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGTGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCGCCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGCTCGAGGCGNOV40c, 246837961Protein SequenceSEQ ID NO: 938250 aaMW at 29980.4kDRGTCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLERQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40d, 246837965SEQ ID NO: 939750 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGTGAGTGGAAACATGCCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGCTCGAGGCGNOV40d, 246837965Protein SequenceSEQ ID NO: 940250 aaMW at 29933.3kDRGTCPRNHSHTRNFYYITMLRDPVSRYLSEWKHAQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40e, 246837975SEQ ID NO: 941750 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGTGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAGCATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCACCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACGCCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGCTCGAGGCGNOV40e, 246837975Protein SequenceSEQ ID NO: 942250 aaMW at 29882.2kDRGTCPRNHSHTPNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERAFNLKFISPFTQFNITRASNVEINEGAHQRIEDLNFLDMQLYEYAKDLFQQRYHHAKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40f, 248061376SEQ ID NO: 943750 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceCGCGGTACCTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGCGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGCTCGAGGCGNOV40f, 248061376Protein SequenceSEQ ID NO: 944250 aaMW at 29961.3kDRGTCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEASEQ ID NO: 9451500 bpNOV40g, SNP13381685 ofORF Start: ATG at 74ORF Stop: TGA at 1490CG56806-01, DNA SequenceSNP Pos: 870SNP Change: A to GCTTCCGAGCGGGCGCCCGTCCGCCCTGCCGCCGCCGCCGCCGCCGCTTCGCCTGCCGGCCTGAGAGCGGGACCATGGATGAAAGGTTCAACAAGTGGCTGCTGACGCCGGTGCTCACTCTCCTCTTCGTGGTCATCATGTACCAGTACGTGTCCCCCTCCTGCACCAGCTCCTGCACCAACTTCGGGGAGCAGCCCCGCGCGGGGGAGGCCGGCCCGCCCGCCGTCCCGGGTCCCGCCCGCCGGGCTCAGGCGCCGCCGGAGGAGTGGGAGCAGAGGAGGCCCCAGTTGCCCCCGCCGCCCCGGGGGCCCCCCGAGGGACCTCGGGGGGCCGCGGCGCCGGAGGAGGAGGACGAGGAGCCCGGAGACCCCCGGGAGGGGGAGGAAGAGGAGGAGGAAGACGAGCCGGACCCCGAGGCCCCGGAAAACGGCTCCCTGCCCCGATTCGTGCCGCGCTTCAACTTCAGCCTGAAGGACCTGACCCGCTTCGTGGATTTCAACATCAAAGGGCGCGACGTGATCGTGTTCCTCCACATCCAGAAGACGGGGGGCACCACTTTCGGCCGGCACCTGGTGAAGAACATCCGGCTGGAGCAGCCTTGTAGCTGCAAAGCGGGTCAGAAGAAGTGCACCTGCCACCGGCCTGGCAAGAAGGAGACGTGGCTCTTCTCCCGCTTCTCCACCGGCTGGAGCTGCGGGCTGCACGCCGACTGGACGGAGCTCACCAACTGCGTGCCGGCCATCATGGAGAAGAAGGACTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGCGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAGAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTCAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGTGACCTCCTGCNOV40g, SNP13381685 ofSEQ ID NO: 946MW at 54999.3kDCG56806-01, Protein SequenceSNP Pos: 266472 aaSNP Change: Lys to ArgMDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARRAQAPPEEWEQRRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWRTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWSEQ ID NO: 9471500 bpNOV40h, SNP13381686 ofORF Start: ATG at 74ORF Stop: TGA at 1490CG56806-01, DNA SequenceSNP Pos: 1190SNP Change: T to CCTTCCGAGCGGGCGCCCGTCCGCCCTGCCGCCGCCGCCGCCGCCGCTTCGCCTGCCGGCCTGAGAGCGGGACCATGGATGAAAGGTTCAACAAGTGGCTGCTGACGCCGGTGCTCACTCTCCTCTTCGTGGTCATCATGTACCAGTACGTGTCCCCCTCCTGCACCAGCTCCTGCACCAACTTCGGGGAGCAGCCCCGCGCGGGGGAGGCCGGCCCGCCCGCCGTCCCGGGTCCCGCCCGCCGGGCTCAGGCGCCGCCGGAGGAGTGGGAGCAGAGGAGGCCCCAGTTGCCCCCGCCGCCCCGGGGGCCCCCCGAGGGACCTCGGGGGGCCGCGGCGCCGGAGGAGGAGGACGAGGAGCCCGGAGACCCCCGGGAGGGGGAGGAAGAGGAGGAGGAAGACGAGCCGGACCCCGAGGCCCCGGAAAACGGCTCCCTGCCCCGATTCGTGCCGCGCTTCAACTTCAGCCTGAAGGACCTGACCCGCTTCGTGGATTTCAACATCAAAGGGCGCGACGTGATCGTGTTCCTCCACATCCAGAAGACGGGGGGCACCACTTTCGGCCGGCACCTGGTGAAGAACATCCGGCTGGAGCAGCCTTGTAGCTGCAAAGCGGGTCAGAAGAAGTGCACCTGCCACCGGCCTGGCAAGAAGGAGACGTGGCTCTTCTCCCGCTTCTCCACCGGCTGGAGCTGCGGGCTGCACGCCGACTGGACGGAGCTCACCAACTGCGTGCCGGCCATCATGGAGAAGAAGGACTGTCCCCGCAACCACAGCCACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGCGAGTGGAAACATGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAGCTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACAACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTTCATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTCTTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGCTCATCTCCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCATTGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCACCACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGCACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCAGGTGGTGAGATGGTGACCTCCTGCNOV40h, SNP13381686 ofSEQ ID NO: 948MW at 54937.3kDCG56806-01, Protein SequenceSNP Pos: 373472 aaSNP Change: Phe to LeuMDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARPAQAPPEEWEQRRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKLISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRW


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 40B.

TABLE 40BComparison of the NOV40 protein sequences.NOV40aMDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARRAQANOV40b------------------------------------------------------------NOV40c------------------------------------------------------------NOV40d------------------------------------------------------------NOV40e------------------------------------------------------------NOV40f------------------------------------------------------------NOV40aPPEEWEQRRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSNOV40b------------------------------------------------------------NOV40c------------------------------------------------------------NOV40d------------------------------------------------------------NOV40e------------------------------------------------------------NOV40f------------------------------------------------------------NOV40aLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKANOV40b------------------------------------------------------------NOV40c------------------------------------------------------------NOV40d------------------------------------------------------------NOV40e------------------------------------------------------------NOV40f------------------------------------------------------------NOV40aGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYNOV40b---------------------------------------------RGTCPRNHSHTRNFYNOV40c---------------------------------------------RGTCPRNHSHTRNFYNOV40d---------------------------------------------RGTCPRNHSHTRNFYNOV40e---------------------------------------------RGTCPRNHSHTRNFYNOV40f---------------------------------------------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--NOV40bHHTKQLEHQRDRQKEREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40cHHTKQLERQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40dHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40eHHAKQLEHQRDRQKEREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40fHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRWLEANOV40a(SEQ ID NO: 934)NOV40b(SEQ ID NO: 936)NOV40c(SEQ ID NO: 938)NOV40d(SEQ ID NO: 940)NOV40e(SEQ ID NO: 942)NOV40f(SEQ ID NO: 944)


Further analysis of the NOV40a protein yielded the following properties shown in Table 40C.

TABLE 40CProtein Sequence Properties NOV40aSignalP analysis:Cleavage site between residues 29 and 30PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 7; pos. chg 2; neg. chg 2H-region:length 31; peak value 11.61PSG score:7.21GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −5.24possible cleavage site: between 28 and 29>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 8-24−2.76PERIPHERALLikelihood =4.77 (at 312)ALOM score: −2.76 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 15Charge difference: −2.0 C(−1.0)-N(1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 8)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):8.01Hyd Moment(95):7.59G content:0D/E content:2S/T content:0Score: −6.61Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 13.6%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: DERFnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):39.1%: mitochondrial34.8%: nuclear13.0%: cytoplasmic 4.3%: extracellular, including cell wall 4.3%: Golgi 4.3%: peroxisomal>> prediction for CG56806-01 is mit (k = 23)


A search of the NOV40a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 40D.

TABLE 40DGeneseq Results for NOV40aNOV40aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAO26610Human 6-OST-3 protein sequence -1 . . . 472471/472 (99%)0.0Homo sapiens, 471 aa.1 . . . 471471/472 (99%)[WO200279258-A2, 10-OCT-2002]AAY83902Mouse HS6ST2 protein - Mus sp, 4701 . . . 472447/472 (94%)0.0aa. [JP2000060566-A, 29-FEB-2000]1 . . . 470458/472 (96%)ABP71364Mouse H6ST-3 protein - Mus sp, 4691 . . . 471446/471 (94%)0.0aa. [WO2003004610-A2, 16-JAN-2003]1 . . . 469457/471 (96%)ABP71362Human AK polypeptide - Homo111 . . . 440 242/330 (73%)e−149sapiens, 460 aa. [WO2003004610-A2,45 . . . 373 285/330 (86%)16-JAN-2003]AAO26608Human 6-OST-2A protein sequence -111 . . . 440 242/330 (73%)e−149Homo sapiens, 465 aa.51 . . . 379 285/330 (86%)[WO200279258-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV40a protein was found to have homology to the proteins shown in the BLASTP data in Table 40E.

TABLE 40EPublic BLASTP Results for NOV40aNOV40aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8IZP7Heparan sulfate 6-O-1 . . . 472469/472 (99%)0.0sulfotransferase 3 - Homo sapiens1 . . . 471469/472 (99%)(Human), 471 aa.Q9QYK4Heparan sulfate 6-sulfotransferase1 . . . 472447/472 (94%)0.03 - Mus musculus (Mouse), 470 aa.1 . . . 470458/472 (96%)AAH47151Hs6st2 protein - Mus musculus111 . . . 440 244/330 (73%)e−149(Mouse), 535 aa (fragment).114 . . . 442 285/330 (85%)Q96SJ4Hypothetical protein FLJ14814 -111 . . . 440 242/330 (73%)e−148Homo sapiens (Human), 459 aa.45 . . . 373 285/330 (86%)Q8NBN4Hypothetical protein111 . . . 440 241/330 (73%)e−148NT2RP2000358 - Homo sapiens51 . . . 379 285/330 (86%)(Human), 465 aa.


PFam analysis predicts that the NOV40a protein contains the domains shown in the Table 40F.

TABLE 40FDomain Analysis of NOV40aPfam DomainNOV40aIdentities/ExpectMatch RegionSimilaritiesValuefor theMatched Region


Example 41

The NOV41 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 41A.

TABLE 41ANOV41 Sequence AnalysisNOV41a, CG56878-01SEQ ID NO: 9492739 bpDNA SequenceORF Start: ATG at 86ORF Stop: TGA at 2090TTGCACTCTCCCACACCCTTTTCTTTTCGTCCGCTCTTCGCTTATTTCTCCCGCCGTCTCCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCTGCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGTAGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAGAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTG&GACCAACACTACACTTGACCCTTCACGGAATCCAGACTCTTCCTGGACTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAACCCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATACGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGAGAGAGCAACGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATTGAATTGCCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCCTGTCCCCTACAATTTGTGCTTCTGAGTTGAGGAGCCTTCACCTCTGTTGCTGAGGAAATGGTAGAATGCTGCCTATCACCTCCAGCACAATCCCAGCGAAAAAGGTGTGAAGCACCCACCATGTTCTTGAACAATCAGGTTTCTAPATAAACAACTGGACCATCANOV41a, CG56878-01Protein SequenceSEQ ID NO: 950668 aaMW at 75659.9kDMAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQAVDSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFNOV41b, 175070399SEQ ID NO: 9511773 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATGGACCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAGAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACCCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41b, 175070399Protein SequenceSEQ ID NO: 952591 aaMW at 67172.0kDGTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKTIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNMDQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLLNKPIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFVDNOV41c, 175070432SEQ ID NO: 9531788 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceTCAGAATTCGCCCTTGGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGGCCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41c, 175070432Protein SequenceSEQ ID NO: 954596 aaMW at 67601.5kDSEFALGTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRGRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFVDNOV41d, 175070419SEQ ID NO: 9551773 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAATAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41d, 175070419Protein SequenceSEQ ID NO: 956591 aaMW at 67124.0kDGTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGIKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDPDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFVDNOV41e, 175070438SEQ ID NO: 9571938 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAGAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41e, 175070438Protein SequenceSEQ ID NO: 958646 aaMW at 73427.1kDGTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFVDNOV41f, 175070408SEQ ID NO: 9591938 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41f, 175070408Protein SequenceSEQ ID NO: 960646 aaMW at 73427.1kDGTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVTPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFVDNOV41g, CG56878-02SEQ ID NO: 9611938 bpDNA SequenceORF Start: at 7ORF Stop: at 1933GGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAGAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41g, CG56878-02Protein SequenceSEQ ID NO: 962642 aaMW at 73054.8kDGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNINQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFNOV41h, CG56878-03SEQ ID NO: 9631773 bpDNA SequenceORF Start: at 7ORF Stop: at 1768GGTACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAATAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAAAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCGTCGACNOV41h, CG56878-03Protein SequenceSEQ ID NO: 964587 aaMW at 66751.6kDGGVGSLNLEELSEMRYGIETLPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGIKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTHNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFNOV41i, CG56878-04SEQ ID NO: 9652702 bpDNA SequenceORF Start: ATG at 86ORF Stop: TGA at 2036TTGCACTCTCCCACACCCTTTTCTTTTCGTCCGCTCTTCGCTTATTTCTCCCGCCGTCTCCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCTGCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATAAGATGAGGATGAACGGCAGTTACTGGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTGAGACCAACACTACACTTGACCCTTCACGGAATCCAGACTCTTCCTGGACTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAACCCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATAGGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGAGAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATGGAATTGCCTGTGGATTCCTTCTCCCTTCCCTGCCCACCGTGTCCTACAATTGTGCTCTGAGTGAGAGCCTTCCTCTCTGCTAGGAAGGTTATGTGCCTTACTCCGCAATCGGAAAGTTAGCCACGTTCTAATCGTTATACAAGGCTAAAAAAAATAAATATTTATACCCGTTTTTCCCTGATTTATTTTTAAATATTATATTATTTTTAATATAATTTGTGGGNOV41i, CG56878-04Protein SequenceSEQ ID NO: 966650 aaMW at 73601.9kDMAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDKMRMNGSYWGEFEKELEGILLPSDRDRLRSETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFSEQ ID NO: 9672739 bpNOV41j, SNP13382511 ofORF Start: ATG at 86ORF Stop: TGA at 2090CG56878-01, DNA SequenceSNP Pos: 2596SNP Change: C to TTTGCACTCTCCCACACCCTTTTCTTTTCGTCCGCTCTTCGCTTATTTCTCCCGCCGTCTCCTCTGCATAAGAAGGGGAACGAAAGATGGCGGCGGAAACGCTGCTGTCCAGTTTGTTAGGACTGCTGCTTCTGGGACTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGTCTCCTCTAAGTACAAACAGCGCTATGAGTGTCGCCTGCCAGCTGGAGCTATTCACTTCCAGCGTGAAAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGTAGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAGAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTGAGACCAACACTACACTTGACCCTTCACGGAATCCAGACTCTTCCTGGACTGGCTTGCCTCCTCCCCACCTCCCCACCCTGGAACCCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATACGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGAGAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATTGAATTGCCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCCTGTCCCCTACAATTTGTGCTTTTGAGTTGAGGAGCCTTCACCTCTGTTGCTGAGGAAATGGTAGAATGCTGCCTATCACCTCCAGCACAATCCCAGCGACAAAGGTGTGAAGCACCCACCATGTTCTTGAACAATCAGGTTTCTAAATAAACAACTGGACCATCANOV41j, SNP13382511 ofMW at 75659.9kDCG56878-01SEQ ID NO: 968668 aaSNP Change: no changeMAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQAVDSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDFSEQ ID NO: 9692739 bpNOV41k, SNP13382506 ofORF Start: ATG at 86ORF Stop: TGA at 2090CG56878-01, DNA SequenceSNP Pos: 2671SNP Change: C to TTTGCACTCTCCCACACCCTTTTCTTTTCGTCCGCTCTTCGCTTATTTCTCCCGCCGTCTCCTCTGCATCTCCTGTTACCCGCAAGTCTGACCGGCGGTGTCGGGAGCCTGAACCTGGAGGAGCTGAGTGAGATGCGTTATGGGATCGAGATCCTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAATCTTCGGACGTGGTGATTGAGGGAGGAGGAAACACCTGCTTACCAAGGGCCTGGGATCCCTGAGTTGTTGAGCCCAATGAGAGATGCTCCCTGCTTGCTGAAGACAAAGGACTGGTGGACATATGAATTCTGTTATGGACGCCACATCCAGCAATACCACATGGAAGATTCAGAGATCAAAGGTGAAGTCCTCTATCTCGGCTACTACCAATCAGCCTTCGACTGGGATGATGAAACAGCCAAGGCCTCCAAGCAGCATCGTCTTAAACGCTACCACAGCCAGACCTATGGCAATGGGTCCAAGTGCGACCTTAATGGGAGGCCCCGGGAGGCCGAGGTTCGGTTCCTCTGTGACGAGGGTGCAGGTATCTCTGGGGACTACATCGATCGCGTGGACGAGCCCTTGTCCTGCTCTTATGTGCTGACCATTCGCACTCCTCGGCTCTGCCCCCACCCTCTCCTCCGGCCCCCACCCAGTGCTGCACCGCAGGCCATCCTCTGTCACCCTTCCCTACAGCCTGAGGAGTACATGGCCTACGTTCAGAGGCAAGCCGTAGACTCAAAGCAGTATGGAGATAAAATCATAGAGGAGCTGCAAGATCTAGGCCCCCAAGTGTGGAGTGAGACCAAGTCTGGGGTGGCACCCCAAAAGATGGCAGGTGCGAGCCCGACCAAGGATGACAGTAAGGACTCAGATTTCTGGAAGATGCTTAATGAGCCAGAGGACCAGGCCCCAGGAGGGGAGGAGGTGCCGGCTGAGGAGCAGGACCCAAGCCCTGAGGCAGCAGATTCAGCTTCTGGTGCTCCCAATGATTTTCAGAACAACGTGCAGGTCAAAGTCATTCGAAGCCCTGCGGATTTGATTCGATTCATAGAGGAGCTGAAAGGTGGAACAAAAAAGGGGAAGCCAAATATAGGCCAAGAGCAGCCTGTGGATGATGCTGCAGAAGTCCCTCAGAGGGAACCAGAGAAGGAAAGGGGTGATCCAGAACGGCAGAGAGAGATGGAAGAAGAGGAGGATGAGGATGAGGATGAGGATGAAGATGAGGATGAACGGCAGTTACTGGGAGAATTTGAGAAGGAACTGGAAGGGATCCTGCTTCCGTCAGACCGAGACCGGCTCCGTTCGGAGGTGAAGGCTGGCATGGAGCGGGAACTGGAGAACATCATCCAGGAGACAGAGAAAGAGCTGGACCCAGATGGGCTGAAGAAGGAGTCAGAGCGGGATCGGGCAATGCTGGCTCTCACATCCACTCTCAACAAACTCATCAAAAGACTGGAGGAAAAACAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGTCCCCAAAAAGCCTCCCCCATCACCCCAACCTACAGAGGAGGATCCTGAGCACAGAGTCCGGGTCCGGGTCACCAAGCTCCGTCTCGGAGGCCCTAATCAGGATCTGACTGTCCTCGAGATGAAACGGGAAAACCCACAGCTGAAACAAATCGAGGGGCTGGTGAAGGAGCTGCTGGAGAGGGAGGGACTCACAGCTGCAGGGAAAATTGAGATCAAAATTGTCCGCCCATGGGCTGAAGGGACTGAAGAGGGTGCACGTTGGCTGACTGATGAGGACACGAGAAACCTCAAGGAGATCTTCTTCAATATCTTGGTGCCGGGAGCTGAAGAGGCCCAGAAGGAACGCCAGCGGCAGAAAGAGCTGGAGAGCAATTACCGCCGGGTGTGGGGCTCTCCAGGTGGGGAGGGCACAGGGGACCTGGACGAATTTGACTTCTG&GACCAACACTACACTTCCTGAGGGCCAAACAGCAGAGTGGAGCTGAGCTGTGGACCTCTCGGGCAACTCTGTGGGTGTGGGGGCCCTGGGTGAATGCTGCTGCCCCTGCTGGCAGCCACCTTGAGACCTCACCGGGCCTGTGATATTTGCTCTCCTGAACTCTCACTCAATCCTCTTCCTCTCCTCTGTGGCTTTTCCTGTTATTGTCCCCTAATGATAGGATATTCCCTGCTGCCTACCTGGAGATTCAGTAGGATCTTTTGAGTGGAGGTGGGTAGAGAGAGCAAGGAGGGCAGGACACTTAGCAGGCACTGAGCAAGCAGGCCCCCACCTGCCCTTAGTGATGTTTGGAGTCGTTTTACCCTCTTCTATTGAATTGCCTTGGGATTTCCTTCTCCCTTTCCCTGCCCACCCTGTCCCCTACCCTCCAGCACAATCCCAGTGAAAAAGGTGTGAAGCACCCACCATGTTCTTGAACAATCAGGTTTCTAAATAAACAACTGGACCATCANOV41k, SNP13382506 ofMW at 75659.9kDCG56878-01, Protein SequenceSEQ ID NO: 970668 aaSNP Change: no changeMAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQAVDSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 41B.

TABLE 41BComparison of the NOV41 protein sequences.NOV41aMAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:950)NOV4lb-------G----------------TGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:952)NOV41c--SEFALG----------------TGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:954)NOV41d-------G----------------TGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:956)NOV41e-------G----------------TGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:958)NOV41f-------G----------------TGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:960)NOV41g-------G------------------GVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:960)NOV41h-------G------------------GVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:962)NOV41iMAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDV(SEQ ID NO:964)NOV41aVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTY(SEQ ID NO:966)NOV41bVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYNOV41cVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYNOV4ldVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYNOV41eVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMPDAPCLLKTKDWWTYNOV41fVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYNOV41gVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMPDAPCLLKTKDWWTYNOV41hVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYNOV41iVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYNOV41aEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41bEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41cEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41dEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41eEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41fEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41gEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41hEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41iEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKNOV41aCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41bCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41cCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41dCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41eCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41fCDLNGRPREAIEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41gCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41hCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41iCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAANOV41aPQAILCHPSLQPEEYMAYVQRQAVDSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41bPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41cPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41dPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41ePQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41fPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41gPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41hPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41iPQAILCHPSLQPEEYMAYVQRQAD-SKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGANOV41aSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41bSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41cSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41dSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41eSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41fSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41gSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41hSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41iSPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVINOV41aRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41bRSPADLIRFIEELKGGTKKGKPNMDQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41cRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41dRSPADLIRFIEELKGGIKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41eRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41fRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41gRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41hRSPADLIRFIEELKGGIKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV41iRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDENOV4laDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41bDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41cDEDEDEDEDERQLLGEFEKELEGILLPSDRGRLRSEVKAGMERELENIIQETEKELDPDGNOV41dDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41eDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41fDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41gDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41hDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGNOV41iDED--KMPMNGSYWGEFEKELEGILLPSDRDRLR-------S--------ETEKELDPDGNOV41aLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHNOV41bLKKESERDRAMLALTSTLNKPIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPT------NOV41cLKKESERDHAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPT------NOV41dLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPT------NOV41eLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHNOV41fLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHNOV41gLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHNOV41hLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPT------NOV41iLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHNOV41aRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWNOV41b-------------------------------------------------GKIEIKIVRPWNOV41c-------------------------------------------------GKIEIKIVRPWNOV41d-------------------------------------------------GKIEIKIVRPWNOV41eRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWNOV41fRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWNOV41gRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWNOV41h-------------------------------------------------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--NOV41bGDLDEFDFVDNOV41cGDLDEFDFVDNOV41dGDLDEFDFVDNOV41eGDLDEFDFVDNOV41fGDLDEFDFVDNOV41gGDLDEFDF--NOV41hGDLDEFDF--NOV41iGDLDEFDF--


Further analysis of the NOV41a protein yielded the following properties shown in Table 41C.

TABLE 41CProtein Sequence Properties NOV41aSignalP analysis:Cleavage site between residues 26 and 27PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 4; pos. chg 0; neg. chg 1H-region:length 29; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 3.65possible cleavage site: between 25 and 26>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 6-22−6.74PERIPHERALLikelihood = 8.49 (at 48)ALOM score: −6.74 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 13Charge difference: −3.0 C(−3.0)-N(0.0)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 6)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):8.03Hyd Moment(95):5.55G content:5D/E content:2S/T content:6Score: −7.42Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: KKHK (3) at 515pat4: KHKK (3) at 516pat4: HKKK (3) at 517pat4: KKKR (5) at 518pat7: nonebipartite: KRLEEKQSPELVKKHKK at 503content of basic residues: 13.6%NLS Score: 1.06KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regions408 E0.52409 R0.52410 Q0.52411 R0.52412 E0.52413 M0.74414 E0.97415 E0.97416 E0.97417 E0.97418 D0.97419 E0.97420 D0.97421 E0.97422 D0.97423 E0.97424 D0.97425 E0.97426 D0.97427 E0.97428 D0.97429 E0.97430 R0.97431 Q0.97432 L0.97433 L0.97434 G0.97435 E0.97436 F0.97437 E0.97438 K0.97439 E0.97440 L0.97441 E0.97442 G0.84443 I0.82total: 36 residuesFinal Results (k = 9/23):43.5%: nuclear26.1%: mitochondrial 8.7%: cytoplasmic 4.3%: extracellular, including cell wall 4.3%: Golgi 4.3%: plasma membrane 4.3%: vesicles of secretory system 4.3%: peroxisomal>> prediction for CG56878-01 is nuc (k = 23)


A search of the NOV41a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 41D.

TABLE 41DGeneseq Results for NOV41aNOV41aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABP42584Human ovarian antigen HOFNF76,3 . . . 120111/118(94%)8e−59SEQ ID NO: 3716 - Homo sapiens, 1406 . . . 123115/118(97%)aa. [WO200200677-A1 , 03-JAN-2002]AAG76089Human colon cancer antigen protein508 . . . 668 100/161(62%)7e−45SEQ ID NO: 6853 - Homo sapiens, 1061 . . . 106102/161(63%)aa. [WO200122920-A2, 05-APR-2001]ABP42294Human ovarian antigen HNHBC18,454 . . . 534 81/81(100%)5e−39SEQ ID NO: 3426 - Homo sapiens, 931 . . . 81 81/81(100%)aa. [WO200200677-A1, 03-JAN-2002]AAG41826Arabidopsis thaliana protein fragment19 . . . 265 83/263(31%)1e−24SEQ ID NO: 52087 - Arabidopsis26 . . . 273 124/263(46%)thaliana, 282 aa. [EP1033405-A2,06-SEP-2000]AAG41828Arabidopsis thaliana protein fragment100 . . . 265 61/169(36%)6e−23SEQ ID NO: 52089 - Arabidopsis48 . . . 208 90/169(53%)thaliana, 217 aa. [EP1033405-A2,06-SEP-2000]


In a BLAST search of public sequence databases, the NOV41a protein was found to have homology to the proteins shown in the BLASTP data in Table 41E.

TABLE 41EPublic BLASTP Results for NOV41aNOV41aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ13438Protein OS-9 precursor - Homo1 . . . 668667/668 (99%)0.0sapiens (Human), 667 aa.1 . . . 667667/668 (99%)Q8IZ58Hypothetical protein - Homo sapiens1 . . . 668603/668 (90%)0.0(Human), 615 aa (fragment).4 . . . 615609/668 (90%)Q9BW99Similar to amplified in osteosarcoma -1 . . . 668603/668 (90%)0.0Homo sapiens (Human), 612 aa.1 . . . 612609/668 (90%)Q8K2C7Hypothetical protein - Mus musculus1 . . . 668489/676 (72%)0.0(Mouse), 617 aa.1 . . . 617544/676 (80%)Q922S5Unknown (Protein for47 . . . 668 447/630 (70%)0.0IMAGE: 3598453) - Mus musculus1 . . . 571501/630 (78%)(Mouse), 571 aa (fragment).


PFam analysis predicts that the NOV41a protein contains the domains shown in the Table 41F.

TABLE 41FDomain Analysis of NOV41aPfam DomainNOV41aIdentities/ExpectMatch RegionSimilaritiesValuefor theMatched Region


Example 42

The NOV42 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 42A.

TABLE 42ANOV42 Sequence AnalysisNOV42a, CG56904-01SEQ ID NO:9711311 bpDNA SequenceORF Start: ATG at 19ORF Stop: TAA at 1282GGAGCTCCCACACTTTCAATGGGGAGGCCCACCCAGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCGATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGGTCAGGCACAGGCTGGTTAGCCACACTGTCCCCAGGGCCCCTCCATCCCCCTGCCTGCCCTGCCACGTCCCAAACATTCTAGTTAGCTGGTAAAGCAATCAGAACAAGAAAATGATAAGANOV42a, CG56904-01SEQ ID NO:972421 aaMW at 46667.3 kDProtein SequenceMGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLDGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLPALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQVRHRLVSHTVPRAPPSPCLPCHVPNILVSWNOV42b, 272355087SEQ ID NO:9731278 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCACCATGGGGAGGCCCACCCAGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGGTCAGGCACAGGCTGGTTAGCCACACTGTCCCCAGGGCCCCTCCATCCCCCTGCCTGCCCTGCCACGTCCCAAACATTCTAGTTAGCTGGGAATTCNOV42b, 272355087SEQ ID NO:974426 aaMW at 47236.9 kDProtein SequenceGSTMGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQVRHRLVSHTVPRAPPSPCLPCHVPNILVSWEFNOV42c, 246862506SEQ ID NO:9751122 bpDNA SequenceORF Start: at 3ORF Stop: at 1122GGATCCACCATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGGTCAGGCACAGGCTGGTTAGCCACACTGTCCCCAGGGCCCCTCCATCCCCCTGCCTGCCCTGCCACGTCCCAAACATTCTAGTTAGCTGGGAATTCAAGGGCGAATTCCAAGCACNOV42c, 246862506SEQ ID NO:976373 aaMW at 41726.5 kDProtein SequenceIHHGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQVRHRLVSHTVPRAPPSPCLPCHVPNILVSWEFKGEFQANOV42d, CG56904-04SEQ ID NO:9771977 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1723ATGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGTCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTAGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCTGATCCTAGAGAGGCCCAGCAGAGCAGCTCAGACTCCTGGGACTCCGCTGGGCCGTGGACTGAGGAGACAACGCCCACCAGGGGCCCTTGGTCTGGCTCTCCTGGGCCTCCAGGGCTGGGCCTGCTCTGCCTGCCACTGGCCGAGACACAGAGGCACACAGCTGGCATACTCCAGGCTCACAGACCACGCCGGCCTGGCGGGACACACCCTACCCCAAACTCCCAACACAGATGGAGGCAGCAACAATAAAGCCANOV42d, CG56904-04SEQ ID NO:978574 aaMW at 63284.0 kDProtein SequenceMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADTAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSNNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQMNOV42e, 268824694SEQ ID NO:9791201 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGTTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCAGCATCCGGCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTAGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCAAGCTTGGCNOV42e, 268824694SEQ ID NO:980400 aaMW at 43934.7 kDProtein SequenceTGSTMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPPACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPKLGNOV42f, 283146542SEQ ID NO:9811744 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTGGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCAAGCTTGGCNOV42f, 283146542SEQ ID NO:982581 aaMW at 63928.7 kDProtein SequenceTGSTMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHEAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLIGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPKLGNOV42g, 308521214SEQ ID NO:9831744 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGCAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTGGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCGAATTCGGCNOV42g, 308521214SEQ ID NO:984581 aaMW at 63963.7 kDProtein SequenceTGSTMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQPNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPEFGNOV42h, CG56904-02SEQ ID NO:9851506 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1504ATGATGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTAGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACGGGCCCCTGANOV42h, CG56904-02SEQ ID NO:986501 aaMW at 55426.1 kDProtein SequenceMMNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPNOV42i, CG56904-03SEQ ID NO:9871239 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1237ATGATGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTAGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCTGANOV42i, CG56904-03SEQ ID NO:988412 aaMW at 45723.1 kDProtein SequenceMMNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGAPDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHKRLRVGDIGPGTWHELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPNOV42 , CG56904-05SEQ ID NO:9891710 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1456ATGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGTCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTAGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCTGATCCTAGAGAGGCCCAGCAGAGCAGCTCAGACTCCTGGGACTCCGCTGGGCCGTGGACTGAGGAGACAACGCCCACCAGGGGCCCTTGGTCTGGCTCTCCTGGGCCTCCAGGGCTGGGCCTGCTCTGCCTGCCACTGGCCGAGACACAGAGGCACACAGCTGGCATACTCCAGGCTCACAGACCACGCCGGCCTGGCGGGACACACCCTACCCCAAACTCCCAACACAGATGGAGGCAGCAACAATAAAGCCANOV42 , CG56904-05SEQ ID NO:990485aaMW at 53581.10 kDProtein SequenceMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWJELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPNOV42k, CG56904-06SEQ ID NO:9911992 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 1738ATGGGGAGGCCCACCCAGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGTCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTAGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCTGATCCTAGAGAGGCCCAGCAGAGCAGCTCAGACTCCTGGGACTCCGCTGGGCCGTGGACTGAGGAGACAACGCCCACCAGGGGCCCTTGGTCTGGCTCTCCTGGGCCTCCAGGGCTGGGCCTGCTCTGCCTGCCACTGGCCGAGACACAGAGGCACACAGCTGGCATACTCCAGGCTCACAGACCACGCCGGCCTGGCGGGACACACCCTACCCCAAACTCCCAACACAGATGCAGGCAGCAACAATAAAGCCANOV42k, CG56904-06SEQ ID NO:992579 aaMW at 63823.6 kDProtein SequenceMGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPPACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPNOV42l, CG56904-07SEQ ID NO:9931278 bpDNA SequenceORF Start: ATG at 10ORF Stop: at 1273GGATCCACCATGGGGAGGCCCACCCAGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGGTCAGGCACAGGCTGGTTAGCCACACTGTCCCCAGGGCCCCTCCATCCCCCTGCCTGCCCTGCCACGTCCCAAACATTCTAGTTAGCTGGGAATTCNOV421, CG56904-07SEQ ID NO:994421 aaMW at 46715.4 kDProtein SequenceMGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQVRHRLVSHTVPRAPPSPCLPCHVPNILVSWNOV42m, CG56904-08SEQ ID NO:9951744 bpDNA SequenceORF Start: ATG at 14ORF Stop: end of sequenceCACCGGATCCACCATGTGGCCGAGCCTGCTGCTGCTCCTGCTGTTGCCGGGGCCCCCGCCCGTCGCCGGCTTGGAAGACGCTGCCTTCCCCCACCTGGGGGAGAGCTTGCAGCCCCTGCCCCGGGCCTGTCCCCTGCGCTGCTCCTGCCCCCGAGTCGACACTGTGGACTGTGATGGCTTGGACCTTCGAGTGTTCCCGGACAACATCACCAGAGCCGCTCAGCACCTCTCCCTGCAGAACAACCAGCTCCAGGAACTCCCCTACAATGAGCTGTCCCGCCTCAGTGGCCTGCGAACCCTCAACCTCCACAACAACCTCATCTCCTCCGAAGGCCTGCCTGACGAGGCCTTCGAGTCCCTCACCCAGCTGCAGCACCTCTGCGTGGCTCACAACAAGCTCTCAGTGGCCCCTCAGTTTCTGCCCCGGTCCCTCCGTGTCGCGGATCTGGCTGCCAACCAAGTGATGGAGATCTTCCCCCTCACCTTTGGGGAGAAGCCGGCACTCAGGTCCGTGTACCTCCACAACAACCAGCTGAGCAACGCTGGCCTGCCCCCCGACGCCTTCCGCGGCTCCGAGGCCATCGCCACCCTCAGCCTCTCCAACAACCAGCTCAGCTACCTGCCGCCCAGCCTGCCGCCCTCACTCGAGCGGCTCCACCTGCAGAACAATCTCATCTCCAAGGTGCCCCGAGGAGCCCTGAGCCGCCAGACTCAACTCCGTGAGCTCTACCTCCAGCACAACCAGCTGACAGACAGTGGCCTGGATGCCACCACCTTCAGCAAGCTGCATAGCCTTGAATACCTGGATCTCTCCCACAACCAGCTGACCACAGTGCCCGCCGGCCTGCCCCGGACCCTGGCTATCCTGCACCTGGGCCGCAACCGCATCCGGCAGGTGGAGGCGGCTCGGCTGCACGGGGCGCGTGGTCTGCGCTATTTGTTGCTGCAGCACAACCAGCTGGGGAGCTCAGGGCTGCCCGCCGGGGCTCTGCGGCCGCTGCGGGGCCTGCACACGCTGCACCTCTATGGCAATGGGCTGGACCGCGTGCCTCCAGCCCTGCCCCGCCGCCTGCGTGCCCTGGTGCTGCCCCACAACCACGTGGCCGCGCTGGGTGCCCGTGACCTGGTCGCCACACCGGGCCTGACGGAGCTTAACCTGGCCTATAACCGCCTGGCCAGCGCCCGTGTGCACCACCGGGCCTTCCGCCGGTTGCGTGCCCTGCGCAGCCTCGACCTGGCAGGGAATCAGCTAACCCGGCTGCCCATGGGCCTGCCCACTGGCCTGCGCACCCTGCAGCTGCAACGCAACCAGCTGCGGATGCTCGAGCCCGAGCCTCTGGCCGGCCTGGACCAACTGCGGGAGCTCAGCCTGGCGCACAACCGGCTCCGGGTCGGCGACATCGGGCCAGGCACCTGGCATGAGCTCCAAGCCCTCCAGATGCTGGACCTCAGCCACAATGAGCTGTCCTTTGTGCCCCCGGACCTGCCTGAGGCCCTGGAGGAGCTGCACCTCGAGGGCAACCGCATCGGCCACGTGGGCCCCGAGGCCTTCCTCAGCACACCCCGCCTGCGTGCCCTCTTCCTCAGGGCCAACAGGCTTCACATGACGAGCATCGCGGCTGAGGCCTTCCTGGGGCTCCCAAACCTGCGTGTGGTGGACACGGCAGGGAATCCGGAGCAGGTCCTGATCCGGCTGCCTCCCACCACCCCACGTGGGCCACGGGCAGGGGGCCCCAAGCTTGGCNOV42m, CG56904-08SEQ ID NO:996577 aaMW at 63582.4 kDProtein SequenceMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVDCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEAFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRIRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQLTRLPMGLPTGLRTLQLQRNQLRMLEPEPLAGLDQLRELSLAHNRLRVGDIGPGTWHELQALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTSIAAEAFLGLPNLRWDTAGNPEQVLIRLPPTTPRGPRAGGPKLG


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 42B.

TABLE 42BComparison of the NOV42 protein sequences.NOV42a---MGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:972)NOV42bGSTMGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:974)NOV42c-----------------------------------------------------------I(SEQ ID NO:976)NOV42d--------MWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:978)NOV42e----TGSTMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:980)NOV42f----TGSTMWPSLLLLLLLPGPPPvAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:982)NOV42g----TGSTMWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:984)NOV42h------------------------------------------------------------(SEQ ID NO:986)NOV42i------------------------------------------------------------(SEQ ID NO:988)NOV42j--------MWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:990)NOV42k---MGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:992)NOV42l---MGRPTQWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:994)NOV42m--------MWPSLLLLLLLPGPPPVAGLEDAAFPHLGESLQPLPRACPLRCSCPRVDTVD(SEQ ID NO:996)NOV42aCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42bCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42cHHGLDLRVFPDNITPAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42dCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42eCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42fCDGLDLRVFPDNITPAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42gCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42h---------------------MMNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42i---------------------MMNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42jCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42kCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42lCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42mCDGLDLRVFPDNITRAAQHLSLQNNQLQELPYNELSRLSGLRTLNLHNNLISSEGLPDEANOV42aFESLTQLQHLCVAHNK--------------------------------------------NOV42bFESLTQLQHLCVAHNK--------------------------------------------NOV42cFESLTQLQHLCVAHNK--------------------------------------------NOV42dFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNNOV42eFESLTQLQHLCVAHNKLSVAPQFLPRSLR-------------------------------NOV42fFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNNOV42gFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNNOV42hFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNNOV42iFESLTQLQHLCVAHN---------------------------------------------NOV42jFESLTQLQHLCVAHNK--------------------------------------------NOV42kFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNNOV42lFESLTQLQHLCVAHNK--------------------------------------------NOV42mFESLTQLQHLCVAHNKLSVAPQFLPRSLRVADLAANQVMEIFPLTFGEKPALRSVYLHNNNOV42a---------------------------------------------NNLISKVPRGALSRQNOV42b---------------------------------------------NNLISKVPRGALSRQNOV42c---------------------------------------------NNLISKVPRGALSRQNOV42dQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQNOV42e----------------------------------------VADLAANQVMEIFPLTFGEKNOV42fQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQNOV42gQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQNOV42hQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQNOV42i------------------------K--------------------NNLISKVPRGALSRQNOV42j---------------------------------------------NNLISKVPRGALSRQNOV42kQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQNOV42l---------------------------------------------NNLISKVPRGALSRQNOV42mQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLISKVPRGALSRQNOV42aTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42bTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42cTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42dTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42ePALRSVYLHNNQLSNAGLPPDAFRGSEAIATLSLSNNQLSYLPPSLPPSLERLHLQNNLINOV42fTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42gTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42hTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGPNRINOV42iTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42jTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42kTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42lTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42mTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTLAILHLGRNRINOV42aRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLDGNGLDRVPPALPRRNOV42bRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42cRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42dRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42eSKVPRGALSRQTQLRELYLQHNQLTDSGLDATTFSKLHSLEYLDLSHNQLTTVPAGLPRTNOV42fRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42gRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42hRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42iRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42jRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42kRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42lRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42mRQVEAARLHGARGLRYLLLQHNQLGSSGLPAGALRPLRGLHTLHLYGNGLDRVPPALPRRNOV42aLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQNOV42bLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQNOV42cLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLHALRSLDLAGNQNOV42dLRALVLPHNHVAALGARDLVATPGLTELNLAYNRLASARVHHRAFRRLRALRSLDLAGNQNOV42eLAILHLGR---------------------------------------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--VPRAPPSPCLPCHVPNILVSW-------------------------NOV42bALQVRHRLVSHT--VPRAPPSPCLPCHVPNILVSWEF-----------------------NOV42cALQVRHRLVSHT--VPRAPPSPCLPCHVPNILVSWEFKGEFQA-----------------NOV42dALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42eNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPKLG--------------------------NOV42fALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42gALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42hALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42iALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42jALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42kALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42lALQVRHRLVSHT--VPEAPPSPCLPCHVPNILVSW-------------------------NOV42mALQMLDLSHNELSFVPPDLPEALEELHLEGNRIGHVGPEAFLSTPRLRALFLRANRLHMTNOV42a---------------------------------------------NOV42c---------------------------------------------NOV42dSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGP---NOV42e---------------------------------------------NOV42fSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPEAGGPKLGNOV42gSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPEFGNOV42hSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGP---NOV42iSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGP---NOV42jSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGP---NOV42kSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGP---NOV42l---------------------------------------------NOV42mSIAAEAFLGLPNLRVVDTAGNPEQVLIRLPPTTPRGPRAGGPKLG


Further analysis of the NOV42a protein yielded the following properties shown in Table 42C.

TABLE 42CProtein Sequence Properties NOV42aSignalP analysis:Cleavage site between residues 25 and 26PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 3; pos. chg 1; neg. chg 0H-region:length 22; peak value 10.55PSG score:6.15GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 2.50possible cleavage site: between 24 and 25>>> Seems to have a cleavable signal peptide (1 to 24)ALOM:Klein et al's method for TM region allocationInit position for calculation: 25Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 4.77 (at 187)ALOM score: 4.77 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 12Charge difference: −4.5 C(−2.5)-N(2.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:1Hyd Moment(75):6.51Hyd Moment(95):8.64G content:3D/E content:1S/T content:2Score: −4.56Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 13 GRP|TQNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PRRLRAL (5) at 266bipartite: nonecontent of basic residues: 10.7%NLS Score: −0.04KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: GRPTnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs:Leucine zipper pattern (PS00029): *** found *** LDLSHNQLTTVPAGLPRTLAIL at 180 LHLDGNGLDRVPPALPRRLRAL at 251 LDLAGNQLTRLPMGLPTGLRTL at 322nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):77.8%: extracellular, including cell wall11.1%: vacuolar11.1%: mitochondrial>> prediction for CG56904-01 is exc (k = 9)


A search of the NOV42a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 42D.

TABLE 42DGeneseq Results for NOV42aNOV42aIdentities/Residues/Similarities forGeneseqProtein/Organism/Length [Patent #,Matchthe MatchedExpectIdentifierDate]ResiduesRegionValueAAU91336Human novel secreted protein7 . . . 421414/415 (99%)0.0LP255(a) - Homo sapiens, 423 aa.9 . . . 423414/415 (99%)[WO200214358-A2, 21-FEB-2002]AAU91340Human novel secreted protein7 . . . 421413/459 (89%)0.0LP255(b) - Homo sapiens, 467 aa.9 . . . 467413/459 (89%)[WO200214358-A2, 21-FEB-2002]AAG65893Amino acid sequence of GSK gene Id4 . . . 405315/494 (63%)e−15390060 - Homo sapiens, 605 aa.30 . . . 523 336/494 (67%)[WO200172961-A2, 04-OCT-2001]AAG65892Amino acid sequence of GSK gene Id63 . . . 405 283/364 (77%)e−15290060 - Homo sapiens, 483 aa.38 . . . 401 300/364 (81%)[WO200172961-A2, 04-OCT-2001]ABU54399Human secreted/transmembrane39 . . . 392 178/443 (40%)6e−79 protein PRO332 - Homo sapiens, 64292 . . . 534 232/443 (52%)aa. [US2002132240-A1, 19-SEP-2002]


In a BLAST search of public sequence databases, the NOV42a protein was found to have homology to the proteins shown in the BLASTP data in Table 42E.

TABLE 42EPublic BLASTP Results for NOV42aNOV42aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueCAD29040Sequence 27 from Patent7 . . . 421414/415 (99%)0.0WO0214358 - Homo sapiens9 . . . 423414/415 (99%)(Human), 423 aa.CAD29044Sequence 35 from Patent7 . . . 421413/459 (89%)0.0WO0214358 - Homo sapiens9 . . . 467413/459 (89%)(Human), 467 aa.Q9H5G9Hypothetical protein FLJ23447 -131 . . . 421 290/291 (99%) e−166Homo sapiens (Human), 363 aa.73 . . . 363 290/291 (99%)Q8N641Similar to hypothetical protein131 . . . 392 141/262 (53%)4e−70FLJ23447 - Homo sapiens (Human),33 . . . 294 175/262 (65%)402 aa.Q8BM45Hypothetical leucine-rich131 . . . 392 140/262 (53%)5e−70repeat/leucine-rich repeat - Mus33 . . . 294 176/262 (66%)musculus (Mouse), 399 aa.


PFam analysis predicts that the NOV42a protein contains the domains shown in the Table 42F.

TABLE 42FDomain Analysis of NOV42aIdentities/PfamNOV42aSimilarities forExpectDomainMatch Regionthe Matched RegionValueLRRNT43 . . . 7111/35 (31%)0.2422/35 (63%)LRR 97 . . . 12211/26 (42%)0.004723/26 (88%)LRR150 . . . 17512/26 (46%)0.05625/26 (96%)LRR176 . . . 19511/25 (44%)0.007920/25 (80%)LRR221 . . . 24610/26 (38%)0.4722/26 (85%)LRR363 . . . 38810/26 (38%)0.4622/26 (85%)


Example 43

The NOV43 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 43A.

TABLE 43ANOV43 Sequence AnalysisNOV43a, CG56914-01SEQ ID NO:9972153 bpDNA SequenceORF Start: ATG at 16ORF Stop: TAA at 2137GATGCTGGCACTTACATGTGTGTGGCCCAGAACCCGGCTGGTACAGCCTTGGGCAAAATCAAGTTAAATGTCCAAGTTCCTCCAGTCATTAGCCCTCATCTAAAGGAATATGTTATTGCTGTGGACAAGCCCATCACGTTATCCTGTGAAGCAGATGGCCTCCCTCCGCCTGACATTACATGGCATAAAGATGGGCGTGCAATTGTGGAATCTATCCGCCAGCGCGTCCTCAGCTCTGGCTCTCTGCAAATAGCATTTGTCCAGCCTGGTGATGCTGGCCATTACACGTGCATGGCAGCCAATGTAGCAGGATCAAGCAGCACAAGCACCAAGCTCACCGTCCATGTACCACCCAGGATCAGAAGTACAGAAGGACACTACACGGTCAATGAGAATTCACAAGCCATTCTTCCATGCGTAGCTGATGGAATCCCCACACCAGCAATTAACTGGAAAAAAGACAATGTTCTTTTAGCTAACTTGTTAGGAAAATACACTGCTGAACCATATGGAGAACTCATTTTAGAAAATGTTGTGCTGGAGGATTCTGGCTTCTATACCTGTGTTGCTAACAATGCTGCAGGTGAAGATACACACACTGTCAGCCTGACTGTGCATGTTCTCCCCACTTTTACTGAACTTCCTGGAGACGTGTCATTAAATAAAGGAGAACAGCTACGATTAAGCTGTAAAGCTACTGGTATTCCATTGCCCAAATTAACATGGACCTTCAATAACAATATTATTCCAGCCCACTTTGACAGTGTGAATGGACACAGTGAACTTGTTATTGAAAGAGTGTCAAAAGAGGATTCAGGTACTTATGTGTGCACCGCAGAGAACAGCGTTGGCTTTGTGAAGGCAATTGGATTTGTTTATGTGAAAGAACCTCCAGTCTTCAAAGGTGATTATCCTTCTAACTGGATTGAACCACTTGGTGGGAATGCAATCCTGAATTGTGAGGTGAAAGGAGACCCCACCCCAACCATCCAGTGGAACAGAAAGGGAGTGGATATTGAAATTAGCCACAGAATCCGGCAACTGGGCAATGGCTCCCTGGCCATCTATGGCACTGTTAATGAAGATGCCGGTGACTATACATGTGTAGCTACCAATGAAGCTGGGGTGGTGGAGCGCAGCATGAGTCTGACTCTGCAAAGTCCTCCTATTATCACTCTTGAGCCAGTGGAAACTGTTATTAATGCTGGTGGCAAAATCATATTGAATTGTCAGGCAACTGGAGAGCCTCAACCAACCATTACATGGTCCCGTCAAGGGCACTCTATTTCCTGGGATGACCGGGTTAACGTGTTGTCCAACAACTCATTATATATTGCTGATGCTCAGAAAGAAGATACCTCTGAATTTGAATGCGTTGCTCGAAACTTAATGGGTTCTGTCCTTGTCAGAGTGCCAGTCATAGTCCAGGTTCATGGTGGATTTTCCCAGTGGTCTGCATGGAGAGCCTGCAGTGTCACCTGTGGAAAAGGCATCCAAAAGAGGAGTCGTCTGTGCAACCAGCCCCTTCCAGCCAATGGTGGGAAGCCCTGCCAAGGTTCAGATTTGGAAATGCGAAACTGTCAAAATAAGCCTTGTCCAGTGGATGGTAGCTGGTCGGAATGGAGTCTTTGGGAAGAATGCACAAGGAGCTGTGGACGCGGCAACCAAACCAGGACCAGGACTTGCAATAATCCATCAGTTCAGCATGGTGGGCGGCCATGTGAAGGGAATGCTGTGGAAATAATTATGTGCAACATTAGGCCTTGCCCAGTTCATGGAGCATGGAGCGCTTGGCAGCCTTGGGGAACATGCAGCGAAAGTTGTGGGAAAGGTACTCAGACAAGAGCAAGACTTTGTAATAACCCACCACCAGCGTTTGGTGGGTCCTACTGTGATGGAGCAGAAACACAGATGCAAGTTTGCAATGAAAGAAATTGTCCAATTCATGGCAAGTGGGCGACTTGGGCCAGTTGGAGTGCCTGTTCTGTGTCATGTGGAGGAGGTGCCAGACAGAGAACAAGGGGCTGCTCCGACCCTGTGCCCCAGTATGGAGGAAGGAAATGCGAAGGGAGTGATGTCCAGAGTGATTTTTGCAACAGTGACCCTTGCCCAAGTGAGTGTTGGAAATACCCATGGTAACTGGAGTCCTTGGANOV43a, CG56914-01SEQ ID NO:998707 aaMW at 76557.7 kDProtein SequenceMCVAQNPAGTALGKIKLNVQVPPVISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQIAFVQPGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPTPTIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLQSPPIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLPANGGKPCQGSDLEMRNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQPWGTCSESCGKGTQTRARLCNNPPPAFGGSYCDGAETQMQVCNERNCPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCEGSDVQSDFCNSDPCPSECWKYPWNOV43b, 262802367SEQ ID NO:9992136 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCACCATGTGTGTGGCCCAGAACCCGGCTGGTACAGCCTTGGGCAAAATCAAGTTAAATGTCCAAGTTCCTCCAGTCATTAGCCCTCATCTAAAGGAATATGTTATTGCTGTGGACAAGCCCATCACGTTATCCTGTGAAGCAGATGGCCTCCCTCCGCCTGACATTACATGGCATAAAGATGGGCGTGCAATTGTGGAATCTATCCGCCAGCGCGTCCTCAGCTCTGGCTCTCTGCAAATAGCATTTGTCCAGCCTGGTGATGCTGGCCATTACACGTGCATGGCAGCCAATGTAGCAGGATCAAGCAGCACAAGCACCAAGCTCACCGTCCATGTACCACCCAGGATCAGAAGTACAGAAGGACACTACACGGTCAATGAGAATTCACAAGCCATTCTTCCATGCGTAGCTGATGGAATCCCCACACCAGCAATTAACTGGAAAAAAGACAATGTTCTTTTAGCTAACTTGTTAGGAAAATACACTGCTGAACCATATGGAGAACTCATTTTAGAAAATGTTGTGCTGGAGGATTCTGGCTTCTATACCTGTGTTGCTAACAATGCTGCAGGTGAAGATACACACACTGTCAGCCTGACTGTGCATGTTCTCCCCACTTTTACTGAACTTCCTGGAGACGTGTCATTAAATAAAGGAGAACAGCTACGATTAAGCTGTAAAGCTACTGGTATTCCATTGCCCAAATTAACATGGACCTTCAATAACAATATTATTCCAGCCCACTTTGACAGTGTGAATGGACACAGTGAACTTGTTATTGAAAGAGTGTCAAAAGAGGATTCAGGTACTTATGTGTGCACCGCAGAGAACAGCGTTGGCTTTGTGAAGGCAATTGGATTTGTTTATGTGAAAGAACCTCCAGTCTTCAAAGGTGATTATCCTTCTAACTGGATTGAACCACTTGGTGGGAATGCAATCCTGAATTGTGAGGTGAAAGGAGACCCCACCCCAACCATCCAGTGGAACAGAAAGGGAGTGGATATTGAAATTAGCCTATACATGTGTAGCTACCAATGAAGCTGGGGTGGTGGAGCGCAGCATGAGTCTGACTCTGCGAAGTCCTCCTATTATCACTCTTGAGCCAGTGGAAACTGTTATTAATGCTGGTGGCAAAATCATATTGAATTGTCAGGCAACTGGAGAGCCTCAACCAACTATTACATGGTCCCGTCAAGGGCACTCTATTTCCTGGGATGACCGGGTTAACGTGTTGTCCAACAACTCATTATATATTGCTGATGCTCAGAAAGAAGATACCTCTGAATTTGAATGTGTTGCTCGAAACTTAATGGGTTCTGTCCTTGTCAGAGTGCCAGTCATAGTCCAGGTTCATGGTGGATTTTCCCAGTGGTCTGCATGGAGAGCCTGCAGTGTCACCTGTGGAAAAGGCATCCAAAAGAGTGCGAAACTGTCAAAATAAGCCTTGTCCAGTGGATGGTAGCTGGTCGGAATGGAGTCTTTGGGAAGAATGCACAAGGAGCTGTGGACGCGGCAACCAAACCAGGACCAGGACTTGCAATAATCCATCAGTTCAGCATGGTGGGCGGCCATGTGAAGGGAATGCTGTGGAAATAATTATGTGCAACATTAGGCCTTGCCCAGTTCATGGAGCATGGAGCGCTTGGCAGCTTTGGGGAACATGCAGCGAAAGTTGTGGGAAAGGTACTCAGACAAGAGCAAGACTTTGTAATAACCCACCACCAGCGTTTGGTGGGTCCTACTGTGATGGAGCAGAAACACAGATGCAAGTTTGCAATGAAAGAAATTGTCCAATTCATGGCAAGTGGGCGACTTGGGCCAGTTGGAGTGCCTGTTCTGTGTCATGTGGAGGAGGTGCCAGACAGAGAACAAGGGGCTGCTCCGACCCTGTGCCCCAGTATGGAGGAAGGAAATGCGAAGGGAGTGATGTCCAGAGTGATTTTTGCAACAGTGACCCTTGCCCAAGTGAGTGTTGGAAATACCCATGGCTCGAGNOV43b, 262802367SEQ ID NO:1000712 aaMW at 77089.2 kDProtein SequenceGSTMCVAQNPAGTALGKIKLNVQVPPVISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQIAFVQPGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPTPTIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLRSPPIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLPANGGKPCQGSDLEMRNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQLWGTCSESCGKGTQTRARLCNNPPPAFGGSYCDGAETQMQVCNERNCPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCEGSDVQSDFCNSDPCPSECWKYPWLENOV43c, CG56914-02SEQ ID NO:100115660 bpDNA SequenceORF Start: ATG at 1649ORF Stop: TAA at 15134GATTAGTGGCATAAACTGTAGGTCAGCTGGTGGAGGCAAGCCAGCAAGGGGCTTCATGGTAACCAGTGGAAACACAAAAATATAAQGGGCTTCTGAGGCGATCGGGCAGTGTCAGTCTTCAGCCGCTAAGCCGAGAAGATCTGGGAAGGAGTCAGTCAGAGAGCCTTGGGCCAGAGTTCCAGGGGCTCTGGGAGTGGCTGCCAGAAAATACCAGAAAATGAAAGGAATTGAAATTAAGAGAAGGGAGAGATTGAAGTGTGGCGCCAAGATTGAAAGGAGAAAGAGGTTGAAGGATAGGGAGGTTGGAGAAGAGAGTAAAAAGAGGCCACTTACTGGATTTGAAATTGAACCACCCAAAGTCACTGTGATGCCCAAGAATCAGTCTTTCACAGGAGGGTCTGAGGTCTCCATCATGTGTTCTGCAACAGGTTATCCCAAACCAAAGATTGCCTGGACCGTTAACGATATGTTTATCGTGGGTTCACACAGGTATAGGATGACCTCAGATGGTACCTTATTTATCAAAAATGCAGCTCCCAAAGATGCAGGGATCTATGGTTGCCTAGCAAAAGCCCCTAAGTTGATGGTAGTTCAGAGTGAGCTCTTGGTTGCCCTTGGGGATATAACCGTTATGGAATGCAAAACCTCTGGTATTCCTCCACCTCAAGTTAAATGGTTCAAAGGAGATCTTGAGTTGAGGCCCTCAACATTCCTCATTATTGACCCTCTCTTGGGACTTTTGAAGATTCAAGAAACACAAGATCTGGATGCTGGCGATTATACCTGTGTAGCCATCAATGAGGCTGGAAGAGCAACAAATTGGCTCAAATGTGACATTACCTTGTTATGTTCAGGGTTATCCAGAACCAACAATCAAATGGCGAAGATTAGACAACATGCCAATTTTCTCAAGACCTTTTTCAGTTAGTTCCATCAGCCAACTAAGAACAGGAGCTCTCTTTATTTTAAACTTATGGGCAAGTGATAAAGGAACCTATATTTGTGAAGCTGAAAACCAGTTTGGAAAGATCCAGTCAGAGACAACAGTAACAGTGACCGGACTTGTTGCTCCACTTATTGGAATCAGCCCTTCAGTGGCCAATGTTATTGAAGGACAGCAGCTTACTTTGCCCTGTACTCTGTTAGCTGGAAATCCCATTCCAGAACGTCGGTGGATTAAGAATTCAGCTATGTTGCTCCAAAATCCTTACATCACTGTGCGCAGTGATGGGAGCCTCCATATTGAAAGAGTTCAGCTTCAGGATGGTGGTGAATATACTTGTGTGGCCAGTAACGTTGCTGGGACCAATAACAAAACTACCTCTGTGGTTGTGCATGTTCTGCCAACCATTCAGCATGGGCAGCAGATACTCAGTACAATTGAAGGCATTCCAGTAACTTTACCATGCAAAGCAAGTGGAAATCCCAAACCGTCTGTCATCTGGTCCAAGGTAAATGATACATCTAGTTATATTTCCTGAAGAGCAGAGTGTGAAGTTCACCTGCAAGTTATCCCTAGTCTTGAGCAGGAGGCTCAGGAGTGGGGCATGGAAAGAAGATAAGTTAATAAAGGATTTCCTATGTGGCTGGACAGATGTGCTAGGAACCCTCCAAGAAACCATATAGATGCACCTCAGAAGGCTCCCTCGGCTTTTCGCCGTGTTTTGCAGAAAGGAGAGCTGATTTCAACCAGCAGTGCTAAGTTTTCAGCAGGAGCTGATGGTAGTCTGTATGTGGTATCACCTGGAGGAGAGGAGAGTGGGGAGTATGTCTGCACTGCCACCAATACAGCCGGCTACGCCAAAAGGAAAGTGCAGCTAACAGTCTATGTAAGGCCCAGAGTGTTTGGAGATCAACGAGGACTGTCCCAGGATAAGCCTGTTGAGATCTCCGTCCTTGCAGGGGAAGAGGTAACACTTCCATGTGAAGTGAAGAGCTTACCTCCACCCATAATTACTTGGGCCAAAGAAACCCAGCTCATCTCACCGTTCTCTCCAAGACACACATTCCTCCCTTCTGGTTCAATGAAGATCACTGAAACCCGCACTTCAGATAGTGGGATGTATCTTTGTGTTGCCACAAATATTGCTGGGAATGTGACTCAGGCTGTCAAATTAAATGTCCATGTTCCTCCAAAGATACAGCGTGGACCTAAACATCTCAAAGTCCAAGTTGGTCAAAGAGTGGATATTCCATGTAATGCTCAAGGGACTCCTCTTCCTGTAATCACCTGGTCCAAAGGTGGAAGCACTATGCTGGTTGATGGAGAGCACCATGTTAGCAATCCAGACGGAACTTTAAGCATCGACCAAGATAACGCTACATGTCCAAGAACCACCCACAGTGGAAGATCTAGAACCTCCATATAACACTACTTTCCAAGAAAGAGTGGCCAATCAACGCATTGAATTTCCATGTCCTGCAAAAGGTACCCCTAAACCAACCATCAAATGGTTACACAATGGTAGAGAGTTGACAGGCAGAGAGCCTGGCATTTCTATCTTGGAAGATGGCACATTGCTGGTTATTGCTTCTGTTACACCCTATGACAATGGGGAGTACATCTGTGTGGCAGTCAATGAAGCTGGAACCACAGAAAGAAAATATAACCTCAAAGTCCATGTTCCTCCAGTAATTAAAGATAAAGAACAAGTTACAAATGTGTCGGTGTTGTTAAATCAGCTGACCAATCTCTTCTGTGAAGTGGAAGGCACTCCATCTCCCATCATTATGTGGTATAAAGATAATGTCCAGGTGACTGAAAGCAGCACTATTCAGACTGTGAACAATGGGAAGATACTGAAGCTCTTCAGAGCCACTCCAGAGGATGCAGGAAGATATTCCTGCAAAGCAATTAATATTGCAGGCACTTCTCAGAAGTACTTTAACATTGATGTGCTAGGTACCAACTTCCCAAATGAAGTCTCAGTTGTCCTCAACCGTGACGTCGCCCTTGAATGCCAGGTCAAAGGCACTCCCTTTCCTGATATTCATTGGTTCAAAGATGGCAATATTAAAGGAGGAAATGTCACCACAGACATATCAGTATTGATCAACAGCCTTATTAAACTGGAATGTGAAACACGGGGACTTCCAATGCCTGCCATTACTTGGTATAAGGACGGGCAGCCAATCATGTCCAGCTCACAAGCACTTTATATTGATAAAGGACAATATCTTCATATTCCTCGAGCACAGGTCTCTGATTCAGCAACATATACGTGTCACGTAGCCAATGTTGCTGGAACTGCTGAAAAATCATTCCATGTGGATGTCTATGTTCCTCCAATGATTCAAGGCAACTTGGCCACGCCTTTGAATAAGCAAGTAGTTATTGCTCATTCTCTGACACTGGAGTGCAAAGCTGCTGGAAACCCTTCTCCCATTCTCACCTGGTTGAAAGATGGTGTACCTGTGAAAGCTAATGACAATATCCGCATAGAAGCTGGTGGGAAGAAACTCGAAATCATGAGTGCCCAAGAAATTGATCGAGGACAGTACATATGCGTGGCTACCAGTGTGGCAGGAGAAAAGGAAATCAAATATGAAGTTGATGTCTTGGTGCCACCAGCTATAGAAGGAGGAGATGAAACATCTTACTTCATTGTGATGGTTAATAACTTACTGGAGCTAGATTGTCATGTGACAGGCTCTCCCCCACCAACTATCATGTGGCTGAAGGATGGCCAGTTAATTGATGAAAGGGATGGATTCAAGATTTTATTAAATGGACGCAAACTGGTTATTGCTCAGGCTCAAGTGTCAAACACAGGCCTTTATCGGTGCATGGCAGCAAATACTGCTGGAGACCACAAGAAGGAATTTGAAGTGACTGTTCATGTTCCTCCAACAATCAAGTCCTCAGGCCTTTCTGAGAGAGTTGTGGTAAAATACAAGCCTGTCGCCTTGCAGTGCATAGCCAATGGGATTCCAAATCCTTCCATTACATGGTTAAAAGATGACCAGCCTGTGAACACTGCCCAAGGAAACCTTAAAATACAGTCTTCTGGTCGAGTTCTACAAATTGCCAAAACCCTGTTGGAAGATGCTGGCAGATACACATGTGTGGCTACCAACGCAGCTGGAGAAACACAACAGCACATTCAACTGCATGTTCATGAACCACCTAGTCTGGAAGATGCTGGAAAAATGCTGAATGAGACTGTGTTGGTGAGCAACCCTGTACAGCTGGAGTGTAAGGCAGCTGGAAATCCTGTGCCTGTTATTACATGGTACAAAGATAATCGTCTACTCTCAGGTTCCACCAGCATGACTTTCTTGAACAGAGGACAGATCATTGATATTGAAAGTGCCCAGATCTCAGATGCTGGCATATATAAATGCGTGGCCATCAACTCAGCTGGAGCTACAGAGTTATTTTACAGTCTGCAAGTTCATGTGGCCCCATCAATTTCTGGCAGCAATAACATGGTGGCAGTGGTGGTTAATAACCCGGTGAGGTTAGAATGTGAAGCCAGAGGTATTCCTGCCCCAAGTCTGACCTGGTTGAAAGATGGGAGTCCTGTTTCTAGTTTTTCTAATGGATTACAGGTTCTCTCTGGTGGTCGAATCCTAGCATTGACCAGTGCACAAATCAGCGACACAGGAAGGTACACCTGCGTGGCAGTGAATGCTGCTGGAGAAAAGCAAAGGGACATTGACCTCCGAGTATATGTTCCGCCAAATATTATGGGAGAAGAACAGAATGTCTCTGTCCTCATTAGCCAAGCTGTGGAATTACTATGTCAAAGTGATGCTATTCCCCCACCTACTCTTACTTGGTTAAAAGACGGCCACCCCTTGCTGAAGAAACCAGGCCTCAGTATATCTGAAAATAGAAGTGTGTTAAAGATTGAAGATGCTCAGGTTCAAGACACTGGTCGTTACACTTGTGAAGCAACAAATGTTGCTGGAAAAACTGAAAAAAACTACAATGTCAACATTTGGGTCCCCCCAAATATTGGTGGTTCTGATGAACTTACTCAACTTACAGTCATTGAAGGGAATCTCATTAGTCTGTTGTGTGAATCAAGTGGTATTCCACCCCCAAATCTCATCTGGAAGAAGAAAGGCTCTCCAGTGCTGACTGATTCCATGGGGCGAGTTAGAATTTTATCTGGGGGCAGGCAATTACAAATTTCAATTGCTGAAAAGTCTGATGCAGCACTCTATTCATGTGTGGCGTCGAATGTTGCTGGGACTGCAAAGAAAGAATACAATCTGCAAGTTTACATTAGACCAACCATAACCAACAGTGGCAGCCACCCTACTGAAATTATTGTGACCCGAGGGAAGAGTATCTCCTTGGAGTGTGAGGTGCAGGGTATTCCACCACCAACAGTGACCTGGATGAAAGATGGCCACCCCTTGATCAAGGCAAAGGGAGTAGAAATACTGGATGAAGGTCACATCCTTCAGCTGAAGAACATTCATGTATCTGACACAGGCCGTTATGTGTGTGTTGCTGTGAATGTAGCAGGAATGACTGACAAAAAATATGACTTAAGTGTCCATGGAGGCAGGATGCTACGGCTGATGCAGACCACAATGGAAGATGCTGGCCAATATACTTGCGTTGTAAGGAATGCAGCTGGTGAAGAAAGAAAAATCTTTGGGCTTTCAGTATTAGTACCACCTCATATTGTGGGTGAAAATACATTGGAAGATGTGAAGGTAAAAGAGAAACAGAGTGTTACGCTGACTTGTGAAGTGACAGGGAATCCAGTGCCAGAAATTACATGGCACAAAGATGGGCAGCCCCTCCAAGAAGATGAAGCCCATCACATTATATCTGGTGGCCGTTTTCTTCAAATTACCAATGTCCAGGTGCCACACACTGGAAGATATACATGTTTGGCTTCCAGTCCAGCTGGCCACAAGAGCAGGAGCTTCAGTCTTAATGTATTTGTATCTCCTACAATTGCTGGTGTAGGTAGTGATGGCAACCCTGAAGATGTCACTGTCATCCTTAACAGCCCTACATCTTTGGTCTGTGAAGCTTATTCATATCCTCCAGCTACCATCACCTGGTTTAAGGATGGCACTCCTTTAGAATCTAACCGAAATATTCGTATTCTTCCAGGAGGCAGAACTCTGCAGATCCTCAATGCACAGGAGGACAATGCTGGAAGATACTCTTGTGTAGCCACGAATGAGGCTGGAGAAATGATAAAGCACTATGAAGTGAAGGTGTACACACTTAATGCTAACATTGTTATAATTGAATCACAGCCCCTTAAATCCGATGATCATGTTAATATTGCTGCGAATGGACACACACTTCAAATAAAGGAGGCTCAAATATCAGACACCGGACGATATACTTGTGTAGCATCTAACATTGCAGGTGAAGATGAGTTGGATTTTGATGTGAATATTCAAGTTCCTCCAAGTTTTCAGAAACTCTGGGAAATAGGAAACATGCTAGATACTGGCAGGAATGGTGAAGCCAAAGATGTGATCATCAACAATCCCATTTCTCTTTACTGTGAGACAAATGCTGCTCCCCCTCCTACACTGACATGGTACAAAGATGGCCACCCTCTGACCTCAAGTGATAAAGTATTGATTTTGCCAGGAGGGCGAGTGTTGCAGATTCCTCGGGCTAAAGTAGAAGATGCTGGGAGATACACATGTGTGGCTGTGAATGAGGCTGGAGAAGATTCCCTTCAATATGATGTCCGTGTACTCGTGCCGCCAATTATCAAGGGAGCAAATAGTGATCTCCCTGAAGAGGTCACCGTGCTGGTGAACAAGAGTGCACTGATAGAGTGTTTATCCAGTGGCAGCCCAGCACCAAGGAATTCCTGGCAGAAAGATGGACAGCCCTTGCTAGAAGATGACCATCATAAATTTCTATCTAATGGACGAATTCTGCAGATTCTGAATACTCAAATAACAGATATCGGCAGGTATGTGTGTGTTGCTGAGAACACAGCTGGGAGTGCCAAAAAATATTTTAACCTCAATGTTCATGTTCCTCCAAGTGTCATTGGTCCTAAATCTGAAAATCTTACCGTCGTGGTGAACAATTTCATCTCTTTGACCTGTGAGGTCTCTGGTTTTCCACCTCGGTCGAACTCTACAGATTATTCGGGCCAAGGTATCAGATGGTGGTGAATACACTTGTATAGCTATCAATCAAGCTGGCGAAAGCAAGAAAAAGTTTTCCCTGACTGTTTATGTGCCCCCAAGCATTAAAGACCATGACAGTGAATCTCTTTCTGTAGTTAATGTAAGAGAGGGAACTTCTGTGTCTTTGGAGTGTGAGTCGAACGCTGTGCCACCTCCAGTCATCACTTGGTATAAGAATGGGCGGATGATAACAGAGTCTACTCATGTGGAGATTTTAGCTGATGGACAAATGCTACACATTAAGAAAGCTGAGGTATCTGACACAGGCCAGTATGTATGTAGAGCTATAAATGTAGCAGGACGGGATGATAAAAATTTCCACCTCAATGTATATGTGCCACCCAGTATTGAAGGACCTGAAAGAGAAGTGATTGTGGAGACGATCAGCAATCCTGTGACATTAACATGTGATGCCACTGGGATCCCACCTCCCACGATAGCATGGTTAAAGAACCACAAGCGCATAGAAAATTCTGACTCACTGGAAGTTCGTATTTTGTCTGGAGGTAGCAAACTCCAGATTGCCCGGTCTCAGCATTCAGATAGTGGAAACTATACATGTATTGCTTCAAATATGGAGGGAAAAGCCCAGAAATATTACTTTCTTTCAATTCAAGTTCCTCCAAGTGTTGCTGGTGCTGAAATTCCAAGTGATGTCAGTGTCCTTCTAGGAGAAAATGTTGAGCTGGTCTGCAATGCAAATGGCATTCCTACTCCACTTATTCAATGGCTTAAAGATGGAAAGCCCATAGCTAGTGGTGAAACAGAAAGAATCCGAGTGAGTGCAAATGGCAGCACATTAAACATTTATGGAGCTCTTACATCTGACACGGGGAAATACACATGTGTTGCTACTAATCCCGCTGGAGAAGAAGACCGAATTTTTAACTTGAATGTCTATGTTACACCTACAATTAGGGGTAATAAAGATGAAGCAGAGAAACTAATGACTTTAGTGGATACTTCAATAAATATTGAATGCAGAGCCACAGGGACGCCTCCACCACAGATAAACTGGCTGAAGAATGGACTTCCTCTGCCTCTCTCCTCCCATATCCGGTTACTGGCAGCAGGACAAGTTATCAGGATTGTGAGAGCTCAGGTGTCTGATGTCGCTGTGTATACTTGTGTGGCCTCCAACAGAGCTGGGGTGGATAATAAGCATTACAATCTTCAAGTGTTTGCACCACCAAATATGGACAATTCAATGGGGACAGAGGAAATCACAGTTCTCAAAGGTAGTTCCACCTCTATGGCATGCATTACTGATGGAACCCCAGCTCCCAGTATGGCCTGGCTTAGAGATGGCCAGCCTCTGGGGCTTGATGCCCATCTGACAGTCAGCACCCATGGAATGGTCCTGCAGCTCCTCAAAGCAGAGACTGAAGATTCGGGAAAGTACACCTGCATTGCCTCAAATGAAGCTGGAGAAGTCAGCAAGCACTTTATCCTCAAGGTCCTAGAACCACCTCACATTAATGGATCTGAAGAACATGAAGAGATATCAGTAATTGTTAATAACCCACTTGAACTTACCTGCATTGCTTCTGGAATCCCAGCCCCTAAAATGACCTGGATGAAAGATGGCCGGCCCCTTCCACAGACGGATCAAGTGCAAACTCTAGGAGGAGGAGAGGTTCTTCGAATTTCTACTGCTCAGGTGGAGGATACAGGAAGATATACATGTCTGGCATCCAGTCCTGCAGGAGATGATGATAAGGAATATCTAGTGAGAGTGCATGTACCTCCTAATATTGCTGGAACTGATGAGCCCCGGGATATCACTGTGTTACGGAACAGACAAGTGACATTGGAATGCAAGTCAGATGCAGTGCCCCCACCTGTAATTACTTGGCTCAGAAATGGAGAACGGTTACAGGCAACACCTCGAGTGCGAATCCTATCTGGAGGGAGATACTTGCAAATCAACAATGCTGACCTAGGTGATACAGCCAATTATACCTGTGTTGCCAGCAACATTGCAGGAAAGACTACAAGAGAATTTATTCTCACTGTAAATGTTCCTCCAAACATAAAGGGGGGCCCCCAGAGCCTTGTAATTCTTTTAAATAAGTCAACTGTATTGGAATGCATCGCTGAAGGTGTCCCAACTCCAAGGATAACATGGAGAAAGGATGGAGCTGTTCTAGCTGGGAATCATGCAAGATATTCCATCTTGGAAAATGGATTCCTTCATATTCAATCAGCACATGTCACTGACACTGGACGGTATTTGTGTATGGCCACCAATGCTGCTGGAACAGATCGCAGGCGAATAGATTTACAGGTCCATGTTCCTCCATCTATTGCTCCGGGTCCTACCAACATGACTGTAATAGTAAATGTTCAAACTACTCTGGCTTGTGAGGCTACTGGGATACCAAAACCATCAATCAATTGGAGAAAAAATGGGCATCTTCTTAATGTGGATCAAAATCAGAACTCATACAGGCTCCTTTCTTCAGGTTCACTAGTAATTATTTCCCCTTCTGTGGATGACACTGCAACCTATGAATGTACTGTGACAAACGGTGCTGGAGATGATAAAAGAACTGTGGATCTCACTGTCCAAGTTCCACCTTCCATAGCTGATGAGCCTACAGATTTCCTAGTAACCAAACATGCCCCAGCAGTAATTACCTGCACTGCTTCGGGAGTTTCTGTCCTCAGGAGCAATTGAAATACTTGCCACCCAATTAAACCATGCTGGAAGATACACTTGTGTCGCTAGGAATGCGGCTGGCTCTGCACATCGACACGTGACCCTTCATGTTCATGAGCCTCCAGTCATTCAGCCCCAACCAAGTGAACTACACGTCATTCTGAACAATCCTATTTTATTACCATGTGAAGCAACAGGGACACCCAGTCCTTTCATTACTTGGCAAAAAGAAGGCATCAATGTTAACACTTCAGGCAGAAACCATGCAGTTCTTCCTAGTGGCGGCTTACAGATCTCCAGAGCTGTCCGAGAGGATGCTGGCACTTACATGTGTGTGGCCCAGAACCCGGCTGGTACAGCCTTGGGCAAAATCAAGTTAAATGTCCAAGTTCCTCCAGTCATTAGCCCTCATCTAAAGGAATATGTTATTGCTGTGGACAAGCCCATCACGTTATCCTGTGAAGCAGATGGCCTCCCTCCGCCTGACATTACATGGCATAAAGATGGGCGTGCAATTGTGGAATCTATCCGCCAGCGCGTCCTCAGCTCTGGCTCTCTGCAAATAACATTTGTCCAGCCTGGTGATGCTGGCCATTACACGTGCATGGCAGCCAATGTAGCAGGATCAAGCAGCACAAGCACCAAGCTCACCGTCCATGTACCACCCAGGATCAGAAGTACAGAAGGACACTACACGGTCAATGAGAATTCACAAGCCATTCTTCCATGCGTAGCTGATGGAATCCCCACACCAGCAATTAACTGGAAAAAAGACAATGTTCTTTTAGCTAACTTGTTAGGAAAATACACTGCTGAACCATATGGAGAACTCATTTTAGAAAATGTTGTGCTGGAGGATTCTGGCTTCTATACCTGTGTTGCTAACAATGCTGCAGGTGAAGATACACACACTGTCAGCCTGACTGTGCATGTTCTCCCCACTTTTACTGAACTTCCTGGAGACGTGTCATTAAATAAAGGAGAACAGCTACGATTAAGCTGTAAAGCTACTGGTATTCCATTGCCCAAATTAACATGGACCTTCAATAACAATATTATTCCAGCCCACTTTGACAGTGTGAATGGACACAGTGAACTTGTTATTGAAAGAGTGTCAAAAGAGGATTCAGGTACTTATGTGTGCACCGCAGAGAACAGCGTTGGCTTTGTGAAGGCAATTGGATTTGTGTATGTGAAAGAACCTCCAGTCTTCAAAGGTGATTATCCTTCTCACTGGATTGAACCACTTGGTGGGAATGCAATCCTGAATTGTGAGGTGAAAGGAGACCCCACCCCAACCATCCAGTGGAACAGAAAGGGAGTGGATATTGAAATTAGCCACAGAATCCGGCAACTGGGCAATGGCTCCCTGGCCATCTATGGCACTGTTAATGAAGATGCCGGTGACTATACATGTGTAGCTACCAATGAAGCTGGGGTGGTGGAGCGCAGCATGAGTCTGACTCTGCAAAGTCCTCCTATTATCACTCTTGAGCCAGTGGAAACTGTTATTAATGCTGGTGGCAAAATCATATTGAATTGTCAGGCAACTGGAGAGCCTCAACCAACCATTACATGGTCCCGTCAAGGGCACTCTATTTCCTGGGATGACCGGGTTAACGTGTTGTCCAACAACTCATTATATATTGCTGATGCTCAGAAAGAAGATACCTCTGAATTTGAATGTGTTGCTCGAAACTTAATGGGTTCTGTCCTTGTCAGAGTGCCAGTCATAGTCCAGGTTCATGGTGGATTTTCCCAGTGGTCTGCATGGAGAGCCTGCAGTGTCACCTGTGGAAAAGGCATCCAAAAGAGGAGTCGTCTGTGCAACCAGCCCCTTCCAGCCAATGGTGGGAAGCCCTGCCAAGGTTCAGATTTGGAAATGCGAAACTGTCAAAATAAGCCTTGTCCAGTGGATGGTAGCTGGTCGGAATGGAGTCTTTGGGAAGAATGCACAAGGAGCTGTGGACGCGGCAACCAAACCAGGACCAGGACTTGCAATAATCCATCAGTTCAGCATGGTGGGCGGCCATGTGAAGGGAATGCTGTGGAAATAATTATGTGCAACATTAGGCCTTGCCCAGTTCATGGAGCATGGAGCGCTTGGCAGCCTTGGGGAACATGCAGCGAAAGTTGTGGGAAAGGTACTCAGACAAGAGCAAGACTTTGTAAAAAGAAATTGTCCAGTTCATGGCAAGTGGGCGACTTGGGCCAGTTGGAGTGCCTGTTCTGTGTCATGTGGAGGAGGTGCCAGACAGAGAACAAGGGGCTGCTCCGACCCTGTGCCCCAGTATGGAGGAAGGAAATGCGAAGGGAGTGATGTCCAGAGTGATTTTTGCAACAGTGACCCTTGCCCAACCCATGGTAACTGGAGTCCTTGGAGTGGCTGGGGAACATGCAGCCGGACGTGTAACGGAGGGCAGATGCGGCGGTACCGCACATGTGATAACCCTCCTCCCTCCAATGGGGGAAGAGCTTGTGGGGGACCAGACTCCCAGATCCAGAGGTGCAACACTGACATGTGTCCTGTGGATGGAAGTTGGGGAAGCTGGCATAGTTGGAGCCAGTGCTCTGCCTCCTGTGGAGGAGGTGAAAAGACTCGGAAGCGGCTGTGCGACCATCCTGTGCCAGTTAAAGGTGGCCGTCCCTGTCCCGGAGACACTACTCAGGTGACCAGGTGCAATGTACAAGCATGTCCAGGTGGGCCCCAGCGAGCCAGAGGAAGTGTTATTGGAAATATTAATGATGTTGAATTTGGAATTGCTTTCCTTAATGCCACAATAACTGATAGCCCTAACTCTGATACTAGAATAATACGTGCCAAAATTACCAATGTACCTCGTAGTCTTGGTTCAGCAATGAGAAAGATAGTTTCTATTCTAAATCCCATTTATTGGACAACAGCAAAGGAAATAGGAGAAGCAGTCAATGGCTTTACCCTCACCAATGCAGTCTTCAAAAGAGAAACTCAAGTGGAATTTGCAACTGGAGAAATCTTGCAGATGAGTCATATTGCCCGGGGCTTGGATTCCGATGGTTCTTTGCTGCTAGATATCGTTGTGAGTGGCTATGTCCTACAGCTTCAGTCACCTGCTGAAGTCACTGTAAAGGATTACACAGAGGACTACATTCAAACAGGTCCTGGGCAGCTGTACGCCTACTCAACCCGGCTGTTCACCATTGATGGCATCAGCATCCCATACACATGGAACCACACCGTTTTCTATGATCAGGCACAGGGAAGAATGCCTTTCTTGGTTGAAACACTTCATGCATCCTCTGTGGAATCTGACTATAACCAGATAGAAGAGACACTGGGTTTTAAAATTCATGCTTCAATATCCAAAGGAGATCGCAGTAATCAGTGCCCCTCCGGGTTTACCTTAGACTCAGTTGGACCTTTTTGTGCTGATGAGGATGAATGTGCAGCAGGGAATCCCTGCTCCCATAGCTGCCACAATGCCATGGGGACTTACTACTGCTCCTGCCCTAAAGGCCTCACCATAGCTGCAGATGGAAGAACTTGTCAAGATATTGATGAGTGTGCTTTGGGTAGGCATACCTGCCACGCTGGTCAGGACTGTGACAATACGATTGGATCTTATCGCTGTGTGGTCCGTTGTGGAAGTGGCTTTCGAAGAACCTCTGATGGGCTGAGTTGTCAAGATATTAATGAATGTCAAGAATCCAGCCCCTGTCACCAGCGCTGTTTCAATGCCATAGGAAGTTTCCATTGTGGATGTGAACCTGGGTATCAGCTCAAAGGCAGAAAATGCATGGATGTGAACGAGTGTAGACAAAATGTATGCAGACCAGATCAGCACTGTAAGAACACCCGTGGTGGCTATAAGTGCATTGATCTTTGTCCAAATGGAATGACCAAGGCAGAAAATGGAACCTGTATTGATATTGATGAATGTAAAGATGGGACCCATCAGTGCAGATATAACCAGATATGTGAGAATACAAGAGGCAGCTATCGTTGTGTATGCCCAAGAGGTTATCGGTCTCAAGGAGTTGGAAGACCCTGCATGGACATTAATGAATGTGAACAAGTGCCTAAACCTTGTGCACATCAGTGCTCCAACACCCCCGGCAGCTTCAAGTGTATCTGTCCACCAGGACAACATTTATTAGGGGACGGGAAATCTTGCGCTGGATTGGAGAGGCTGCCAAATTATGGCACTCAATACAGTAGCTATAACCTTGCACGGTTCTCCCCTGTGAGAAACAACTATCAACCTCAACAGCATTACAGACAGTACTCACATCTCTACAGCTCCTACTCAGAGTATAGAAACAGCAGAACATCTCTCTCCAGGACTAGAAGGACTATTAGGAAAACTTGCCCTGAAGGCTCTGAGGCAAGCCATGACACATGTGTAGATATTGATGAATGTGAAAATACAGATGCCTGCCAGCATGAGTGTAAGAATACCTTTGGAAGTTATCAGTGCATCTGCCCACCTGGCTATCAACTCACACACAATGGAAAGACATGCCAAGATATCGATGAATGTCTGGAGCAGAATGTGCACTGTGGACCCAATCGCATGTGCTTCAACATGAGAGGAAGCTACCAGTGCATCGATACACCCTGTCCACCCAACTACCAACGGGATCCTGTTTCAGGGTTCTGCCTCAAGAACTGTCCACCCAATGATTTGGAATGTGCCTTGAGCCCATATGCCTTGGAATACAAACTCGTCTCCCTCCCATTTGGAATAGCCACCAATCAAGATTTAATCCGGCTGGTTGCATACACACAGGATGGAGTGATGCATCCCAGGACAACTTTCCTCATGGTAGATGAGGAACAGACTGTTCCTTTTGCCTTGAGGGATGAAAACCTGAAAGGAGTGGTGTATACAACACGACCACTACGAGAAGCAGAGACCTACCGCATGAGGGTCCGAGCCTCATCCTACAGTGCCAATGGGACCATTGAATATCAGACCACATTCATAGTTTATATAGCTGTGTCCGCCTATCCATACTAAGGAACTCTCCAAAGCCTATTCCACATATTTAAACCGCATTAATCATGGCAATCAAGCCCCCTTCCAGATTACTGTCTCTTGAACAGTTGCAATCTTGGCAGCTTGAAAATGGTGCTACACTCTGTTTTGTGTGCCTTCCTTGGTACTTCTGAGGTATTTTCATGATCCCACCATGGTCATATCTTGAAGTATGGTCTAGAAAAGTCCCTTATTATTTTATTTATTACACTGCAGCACTTACTTCCCAAAGATTATTCTGAACATCTAACAGGACATATCAGTGATGGTTTACAGTAGTGTAGTACCTAAGATCATTTTCCTGAAAGCCAAACCAAACAACGAAAAACAAGAACAACTAATTCAGAATCAAATAGAGTTTTTGAGCATTTGACTATTTTTAGAATCATAAAATTAGTTACTAAGTATTTTGATCAAAGCTTATAAAATAACTTACGGAGATTTTTGTAAGTATTGATACATTATAATAGGACTTGCCTATTTTCATTTTTAAGAAGAAAAACCCGNOV43c, CG56914-02SEQ ID NO:10024495 aaMW at 488830.5 kDProtein SequenceMWLDRCARNPPRNHIDAPQKAPSAFRRVLQKGELISTSSAKFSAGADGSLYVVSPGGEESGEYVCTATNTAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEVTLPCEVKSLPPPTITWAKETQLISPFSPRHTFLPSGSMKITETRTSDSGMYLCVATNIAGNVTQAVKLNVHVPPKIQRGPKHLKVQVGQRVDIPCNAQGTPLPVITWSKGGSTMLVDGEHHVSNPDGTLSIDQATPSDAGIYTCVATNIAGTDETEITLHVQEPPTVEDLEPPYNTTFQERVANQRIEFPCPAKGTPKPTIKWLHNGRELTGREPGISILEDGTLLVIASVTPYDNGEYICVAVNEAGTTERKYNLKVHVPPVIKDKEQVTNVSVLLNQLTNLFCEVEGTPSPIIMWYKDNVQVTESSTIQTVNNGKILKLFRATPEDAGRYSCKAINIAGTSQKYFNIDVLGTNFPNEVSVVLNRDVALECQVKGTPFPDIHWFKDGNIKGGNVTTDISVLINSLIKLECETRGLPMPAITWYKDGQPIMSSSQALYIDKGQYLHIPRAQVSDSATYTCHVANVAGTAEKSFHVDVYVPPMIEGNLATPLMKQVVIAHSLTLECKAAGNPSPILTWLKDGVPVKANDNIRIEAGGKKLEIMSAQEIDRGQYICVATSVAGEKEIKYEVDVLVPPAIEGGDETSYFIVMVNNLLELDCHVTGSPPPTIMWLKDGQLIDERDGFKILLNGRKLVIAQAQVSNTGLYRCMAANTAGDHKKEFEVTVHVPPTIKSSGLSERVVVKYKPVALQCIANGIPNPSITWLKDDQPVNTAQGNLKIQSSGRVLQIAKTLLEDAGRYTCVATNAAGETQQHIQLHVHEPPSLEDAGKMLNETVLVSNPVQLECKAAGNPVPVITWYKDNRLLSGSTSMTFLNRGQIIDIESAQISDAGIYKCVAINSAGATELFYSLQVHVAPSISGSNNMVAVVVNNPVRLECEARGIPAPSLTWLKDGSPVSSFSNGLQVLSGGRILALTSAQISDTGRYTCVAVNAAGEKQRDIDLRVYVPPNIMGEEQNVSVLISQAVELLCQSDAIPPPTLTWLKDGHPLLKKPGLSISENRSVLKIEDAQVQDTGRYTCEATNVAGKTEKNYNVNIWVPPNIGGSDELTQLTVIEGNLISLLCESSGIPPPNLIWKKKGSPVLTDSMGRVRTLSGGRQLQISIAEKSDAALYSCVASNVAGTAKKEYNLQVYIRPTITNSGSHPTEIIVTRGKSISLECEVQGIPPPTVTWMKDGHPLIKAKGVEILDEGHILQLKNIHVSDTGRYVCVAVNVAGMTDKKYDLSVHGGRMLRLMQTTMEDAGQYTCVVRNAAGEERKIFGLSVLVPPHIVGENTLEDVKVKEKQSVTLTCEVTGNPVPEITWHKDGQPLQEDEAHHIISGGRFLQITNVQVPHTGRYTCLASSPAGHKSRSFSLNVFVSPTIAGVGSDGNPEDVTVILNSPTSLVCEAYSYPPATITWFKDGTPLESNPNIRILPGGRTLQILNAQEDNAGRYSCVATNEAGEMIKHYEVKVYTLNANIVIIESQPLKSDDHVNIAANGHTLQIKEAQISDTGRYTCVASNIAGEDELDFDVNIQVPPSFQKLWEIGNMLDTGRNGEAKDVIINNPISLYCETNAAPPPTLTWYKDGHPLTSSDKVLILPGGRVLQIPRAKVEDAGRYTCVAVNEAGEDSLQYDVRVLVPPIIKGANSDLPEEVTVLVNKSALIECLSSGSPAPRNSWQKDGQPLLEDDHHKFLSNGRILQILNTQITDIGRYVCVAENTAGSAKKYFNLNVHVPPSVIGPKSENLTVVVNNFISLTCEVSGFPPPDLSWLKNEQPIKLNTNTLIVPGGRTLQIIRAKVSDGGEYTCIAINQAGESKKKFSLTVYVPPSIKDHDSESLSVVNVREGTSVSLECESNAVPPPVITWYKNGRMITESTHVEILANGQMLHIKKAEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPSIEGPEREVIVETISNPVTLTCDATGIPPPTIAWLKNHKRIENSDSLEVRILSGGSKLQIARSQHSDSGNYTCIASNMEGKAQKYYFLSIQVPPSVAGAEIPSDVSVLLGENVELVCNANGIPTPLIQWLKDGKPIASGETERIRVSANGSTLNIYGALTSDTGKYTCVATNPAGEEDRIFNLNVYVTPTIRGNKDEAEKLMTLVDTSINIECRATGTPPPQINWLKNGLPLPLSSHIRLLAAGQVIRIVRAQVSDVAVYTCVASNRAGVDNKHYNLQVFAPPNMDNSMGTEEITVLKGSSTSMACITDGTPAPSMAWLRDGQPLGLDAHLTVSTHGMVLQLLKAETEDSGKYTCIASNEAGEVSKHFILKVLEPPHINGSEEHEEISVIVNNPLELTCIASGIPAPKMTWMKDGRPLPQTDQVQTLGGGEVLRISTAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGTDEPRDITVLRNRQVTLECKSDAVPPPVITWLRNGERLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTTREFILTVNVPPNIKGGPQSLVILLNKSTVLECIAEGVPTPRITWRKDGAVLAGNHARYSILENGFLHIQSAHVTDTGRYLCMATNAAGTDRRRIDLQVHVPPSIAPGPTNMTVIVNVQTTLACEATGIPKPSINWRKNGHLLNVDQNQNSYRLLSSGSLVIISPSVDDTATYECTVTNGAGDDKRTVDLTVQVPPSIADEPTDFLVTKHAPAVITCTASGVPFPSIHWTKNGIRLLPRGDGYRILSSGAIEILATQLNHAGRYTCVARNAAGSAHRHVTLHVHEPPVIQPQPSELHVILNNPILLPCEATGTPSPFITWQKEGINVNTSGRNHAVLPSGGLQISRAVREDAGTYMCVAQNPAGTALGKIKLNVQVPPVISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQITFVQPGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSHWIEPLGGNAILNCEVKGDPTPTIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLQSPPIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLPANGGKPCQGSDLEMENCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQPWGTCSESCGKGTQTRAELCNNPPPAFGGSYCDGAETQMQVCNERNCPVHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCEGSDVQSDFCNSDPCPTHGNWSPWSGWGTCSRTCNGGQMRRYRTCDNPPPSNGGRACGGPDSQIQRCNTDMCPVDGSWGSWHSWSQCSASCGGGEKTRKRLCDHPVPVKGGRPCPGDTTQVTRCNVQACPGGPQRARGSVIGNINDVEFGIAFLNATITDSPNSDTRIIRAKITNVPRSLGSAMRKIVSILNPIYWTTAKEIGEAVNGFTLTNAVFKRETQVEFATGEILQMSHIARGLDSDGSLLLDIVVSGYVLQLQSPAEVTVKDYTEDYIQTGPGQLYAYSTRLFTIDGISIPYTWNHTVFYDQAQGRMPFLVETLHASSVESDYNQIEETLGFKIHASISKGDRSNQCPSGFTLDSVGPFCADEDECAAGNPCSHSCHNAMGTYYCSCPKGLTIAADGRTCQDIDECALGRHTCHAGQDCDNTIGSYRCVVRCGSGFRRTSDGLSCQDINECQESSPCHQRCFNAIGSFHCGCEPGYQLKGRKCMDVNECRQNVCRPDQHCKNTRGGYKCIDLCPNGMTKAENGTCIDIDECKDGTHQCRYNQICENTRGSYRCVCPRGYRSQGVGRPCMDINECEQVPKPCAHQCSNTPGSFKCICPPGQHLLGDGKSCAGLERLPNYGTQYSSYNLARFSPVRNNYQPQQHYRQYSHLYSSYSEYRNSRTSLSRTRRTIRKTCPEGSEASHDTCVDIDECENTDACQHECKNTFGSYQCICPPGYQLTHNGKTCQDIDECLEQNVHCGPNRMCFNMRGSYQCIDTPCPPNYQRDPVSGFCLKNCPPNDLECALSPYALEYKLVSLPFGIATNQDLIRLVAYTQDGVMHPRTTFLMVDEEQTVPFALRDENLKGVVYTTRPLREAETYRMRVRASSYSANGTIEYQTTFIVYIAVSAYPYNOV43d, CG56914-03SEQ ID NO:10036343 bpDNA SequenceORF Start: ATG at 105ORF Stop: TAA at 5811AACCACCTCACATTAATGGATCTGAAGAACATGAAGAGATATCAGTAATTGTTAATAACCCACTTGAACTTACCTGCATTGCTTCTGGAATCCCAGCCCCTAAAATGACCTGGATGAAAGATGGCCGGCCCCTTCCACAGACGGATCAAGTGCAAACTCTAGGAGGAGGAGAGGTTCTTCGAATTTCTACTGCTCAGGTGGAGGATACAGGAAGATATACATGTCTGGCATCCAGTCCTGCAGGAGATGATGATAAGGAATATCTAGTGAGAGTGCATGTACCTCCTAATATTGCTGGAACTGATGAGCCCCGGGATATCACTGTGTTACGGAACAGACAAGTGACATTGGAATGCAAGTCAGATGCAGTGCCCCCACCTGTAATTACTTGGCTCAGAAATGGAGAACGGTTACAGGCAACACCTCGAGTGCGAATCCTATCTGGAGGGAGATACTTGCAAATCAACAATGCTGACCTAGGTGATACAGCCAATTATACCTGTGTTGCCAGCAACATTGCAGGAAAGACTACAAGAGAATTTATTCTCACTGTAAATGTTCCTCCAAACATAAAGGGGGGCCCCCAGAGCCTTGTAATTCTTTTAAATAAGTCAACTGTATTGGAATGCATCGCTGAAGGTGTGCCAACTCCAAGGATAACATGGAGAAAGGATGGAGCTGTTCTAGCTGGGAATCATGCAAGATATTCCATCTTGGAAAATGGATTCCTTCATATTCAATCAGCACATGTCACTGACACTGGACGGTATTTGTGTATGGCCACCAATGCTGCTGGAACAGATCGCAGGCGAATAGATTTACAGGTCCATGGTTCACTAGTAATTATTTCCCCTTCTGTGGATGACACTGCAACCTATGAATGTACTGTGACAAACGGTGCTGGAGATGATAAAAGAACTGTGGATCTCACTGTCCAAGTTCCACCTTCCATAGCTGATGAGCCTACAGATTTCCTAGTAACCAAACATGCCCCAGCAGTAATTACCTGCACTGCTTCGGGAGTTCCATTTCCCTCAATTCACTGGACCAAAAATGGTATAAGACTGCTTCCCAGGGGAGATGGCTATAGAATTCTGTCCTCAGGAGCAATTGAAATACTTGCCACCCAATTAAACCATGCTGGAAGATACACTTGTGTCGCTAGGAATGCGGCTGGCTCTGCACATCGACACGTGACCCTTCATGTTCATGAGCCTCCAGTCATTCAGCCCCAACCAAGTGAACTACACGTCATTCTGAACAATCCTATTTTATTACCATGTGAAGCAACAGGGACACCCAGTCCTTTCATTACTTGGCAAAAAGAAGGCATCAATGTTAACACTTCAGGCAGAAACCATGCAGTTCTTCCTAGTGGCGGCTTACAGATCTCCAGAGCTGTCCGAGAGGATGCTGGCACTTACATGTGTGTGGCCCAGAACCCGGCTGGTACAGCCTTGGGCAAAATCAAGTTAAATGTCCAAGTTCCTCCAGTCATTAGCCCTCATCTAAAGGAATATGTTATTGCTGTGGACAAGCCCATCACGTTATCCTGTGAAGCAGATGGCCTCCCTCCGCCTGACATTACATGGCATAAAGATGGGCGTGCAATTGTGGAATCTATCCGCCAGCGCGTCCTCAGCTCTGGCTCTCTGCAAATAGCATTTGTCCAGCCTGGTGATGCTGGCCATTACACGTGCATGGCAGCCAATGTAGCAGGATCAAGCAGCACAAGCACCAAGCTCACCGTCCATGTACCACCCAGGATCAGAAGTACAGAAGGACACTACACGGTCAATGAGAATTCACAAGCCATTCTTCCATGCGTAGCTGATGGAATCCCCACACCAGCAATTAACTGGAAAAAAGACAATGTTCTTTTAGCTAACTTGTTAGGAAAATACACTGCTGAACCATATGGAGAACTCATTTTAGAAAATGTTGTGCTGGAGGATTCTGGCTTCTATACCTGTGTTGCTAACAATGCTGCAGGTGAAGATACACACACTGTCAGCCTGACTGTGCATGTTCTCCCCACTTTTACTGAACTTCCTGGAGACGTGTCATTAAATAAAGGAGAACAGCTACGATTAAGCTGTAAAGCTACTGGTATTCCATTGCCCAAATTAACATGGACCTTCAATAACAATATTATTCCAGCCCACTTTGACAGTGTGAATGGACACAGTGAACTTGTTATTGAAAGAGTGTCAAAAGAGGATTCAGGTACTTATGTGTGCACCGCAGAGAACAGCGTTGGCTTTGTGAAGGCAATTGGATTTGTTTATGTGAAAGAACCTCCAGTCTTCAAAGGTGATTATCCTTCTAACTGGATTGAACCACTTGGTGGGAATGCAATCCTGAATTGTGAGGTGAAAGGAGACCCCACCCCAACCATCCAGTGGAACAGAAAGGGAGTGGATATTGAAATTAGCCACAGAATCCGGCAACTGGGCAATGGCTCCCTGGCCATCTATGGCACTGTTAATGAAGATGCCGGTGACTATACATGTGTAGCTACCAATGAAGCTGGGGTGGTGGAGCGCAGCATGAGTCTGACTCTGCAAAGTCCTCCTATTATCACTCTTGAGCCAGTGGAAACTGTTATTAATGCTGGTGGCAAAATCATATTGAATTGTCAGGCAACTGGAGAGCCTCAACCAACCATTACATGGTCCCGTCAAGGGCACTCTATTTCCTGGGATGACCGGGTTAACGTGTTGTCCAACAACTCATTATATATTGCTGATGCTCAGAAAGAAGATACCTCTGAATTTGAATGCGTTGCTCGAAACTTAATGGGTTCTGTCCTTGTCAGAGTGCCAGTCATAGTCCAGGTTCATGGTGGATTTTCCCAGTGGTCTGCATGGAGAGCCTGCAGTGTCACCTGTGGAAAAGGCATCCAAAAGAGGAGTCGTCTGTGCAACCAGCCCCTTCCAGCCAATGGTGGGAAGCCCTGCCAAGGTTCAGATTTGGAAATGCGAAACTGTCAAAATAAGCCTTGTCCAGTGGATGGTAGCTGGTCGGAATGGAGTCTTTGGGAAGAATGCACAAGGAGCTGTGGACGCGGCAACCAAACCAGGACCAGGACTTGCAATAATCCATCAGTTCAGCATGGTGGGCGGCCATGTGAAGGGAATGCTGTGGAAATAATTATGTGCAACATTAGGCCTTGCCCAGTTCATGGAGCATGGAGCGCTTGGCAGCCTTGGGGAACATGCAGCGAAAGTTGTGGGAAAGGTACTCAGACAAGAGCAAGACTTTGTAATAACCCACCACCAGCGTTTGGTGGGTCCTACTGTGATGGAGCAGAAACACAGATGCAAGTTTGCAATGAAAGAAATTGTCCAATTCATGGCAAGTGGGCGACTTGGGCCAGTTGGAGTGCCTGTTCTGTGTCATGTGGAGGAGGTGCCAGACAGAGAACAAGGGGCTGCTCCGACCCTGTGCCCCAGTATGGAGGAAGGAAATGCGAAGGGAGTGATGTCCAGAGTGATTTTTGCAACAGTGACCCTTGCCCAACCCATGGTAACTGGAGTCCTTGGAGTGGCTGGGGAACATGCAGCCGGACGTGTAACGGAGGGCAGATGCGGCGGTACCGCACATGTGATAACCCTCCTCCCTCCAATGGGGGAAGAGCTTGTGGGGGACCAGACTCCCAGATCCAGAGGTGCAACACTGACATGTGTCCTGTGGATGGAAGTTGGGGAAGCTGGCATAGTTGGAGCCAGTGCTCTGCCTCCTGTGGAGGAGGTGAAAAGACTCGGAAGCGGCTGTGCGACCATCCTGTGCCAGTTAAAGGTGGCCGTCCTTGTCCCGGAGACACTACTCAGGTGACCAGGTGCAATGTACAAGCATGTCCAGGTGGGCCCCAGCGAGCCAGAGGAAGTGTTATTGGAAATATTAATGATGTTGAATTTGGAATTGCTTTCCTTAATGCCACAATAACTGATAGCCCTAACTCTGATACTAGAATAATACGTGCCAAAATTACCAATGTACCTCGTAGTCTTGGTTCAGCAATGAGAAAGATAGTTTCTATTCTAAATCCCATTTATTGGACAACAGCAAAGGAAATAGGAGAAGCAGTCAATGGCTTTACCCTCACCAATGCAGTCTTCAAAAGAGAAACTCAAGTGGAATTTGCAACTGGAGAAATCTTGCAGATGAGTCATATTGCCCGGGGCTTGGATTCCGATGGTTCTTTGCTGCTAGATATCGTTGTGAGTGGCTATGTCCTACAGCTTCAGTCACCTGCTGAAGTCACTGTAAAGGATTACACAGAGGACTACATTCAAACAGGTCCTGGGCAGCTGTACGCCTACTCAACCCGGCTGTTCACCATTGATGGCATCAGCATCCCATACACATGGAACCACACCGTTTTCTATGATCAGGCACAGGGAAGAATGCCTTTCTTGGTTGAAACACTTCATGCATCCTCTGTGGAATCTGACTATAACCAGATAGAAGAGACACTGGGTTTTAAAATTCATGCTTCAATATCCAAAGGAGATCGCAGTAATCAGTGCCCCTCCGGGTTTACCTTAGACTCAGTTGGACCTTTTTGTGCTGATGAGGATGAATGTGCAGCAGGGAATCCCTGCTCCCATAGCTGCCACAATGCCATGGGGACTTACTACTGCTCCTGCCCTAAAGGCCTCACCATAGCTGCAGATGGAAGAACTTGTCAAGATATTGATGAGTGTGCTTTGGGTAGGCATACCTGCCACGCTGGTCAGGACTGTGACAATACGATTGGATCTTATCGCTGTGTGGTCCGTTGTGGAAGTGGCTTTCGAAGAACCTCTGATGGGCTGAGTTGTCAAGATATTAATGAATGTCAAGAATCCAGCCCCTGTCACCAGCGCTGTTTCAATGCCATAGGAAGTTTCCATTGTGGATGTGAACCTGGGTATCAGCTCAAAGGCAGAAAATGCATGGATGTGAACGAGTGTAGACAAAATGTATGCAGACCAGATCAGCACTGTAAGAACACCCGTGGTGGCTATAAGTGCATTGATCTTTGTCCAAATGGAATGACCAAGGCAGAAAATGGAACCTGTATTGATATTGATGAATGTAAAGATGGGACCCATCAGTGCAGATATAACCAGATATGTGAGAATACAAGAGGCAGCTATCGTTGTGTATGCCCAAGAGGTTATCGGTCTCAAGGAGTTGGAAGACCCTGCATGGATATTGATGAATGTGAAAATACAGATGCCTGCCTGCATGAGTGTAAGAATACCTTTGGAAGTTATCAGTGCATCTGCCCACCTGGCTATCAACTCACACACAATGGAAAGACATGCCAAGATATCGATGAATGTCTGGAGCAGAATGTGCACTGTGGACCCAATCGCATGTGCTTCAACATGAGAGGAAGCTACCAGTGCATCGATACACCCTGTCCACCCAACTACCAACGGGATCCTGCTTCAGGGTTCTGCCTCAAGAACTGTCCACCCAATGATTTGGAATGTGCCTTGAGCCCATATGCCTTGGAATACAAACTCGTCTCCCTCCCATTTGGAATAGCCACCAATCAAGATTTAATCCGGCTGGTTGCATACACACAGGATGGAGTGATGCATCCCAGGACAACTTTCCTCATGGTAGATGAGGAACAGACTGTTCCTTTTGCCTTGAGGGATGAAAACCTGAAAGGAGTGGTGTATACAACACGACCACTACGAGAAGCAGAGACCTACCGCATGAGGGTCCGAGCCTCATCCTACAGTGCCAATGGGACCATTGAATATCAGACCACATTCATAGTTTATATAGCTGTGTCCGCCTATCCATACTAAGGAACTCTCCAAAGCCTATTCCACATATTTAAACCGCATTAATCATGGCAATCAAGCCCCCTTCCAGATTACTGTCTCTTGAACAGTTGCAATCTTGGCAGCTTGAAAATGGTGCTACACTCTGTTTTGTGTGCCTTCCTTGGTACTTCTGAGGTATTTTCATGATCCCACCATGGTCATATCTTGAAGTATGGTCTAGAAAAGTCCCTTATTATTTTATTTATTACACTGGAGCAGTTACTTCCCAAAGATTATTCTGAACATCTAACAGGACATATCAGTGATGGTTTACAGTAGTGTAGTACCTAAGATCATTTTCCTGAAAGCCAAACCAAACAACGAAAAACAAGAACAACTAATTCAGAATCAAATAGAGTTTTTGAGCATTTGACTATTTTTAGAATCATAAAATTAGTTACTAAGTATTTTGATCAAAGCTTATAAAATAACTTACGGAGATTTTTGTAAGTATTGATACATTATAATAGGACTTGCCTATTTTCATTTTTAAGAAGAAAAACACCACTCATNOV43d, CG56914-03SEQ ID NO:10041902 aaMW at 207163.2 kDProtein SequenceMTWMKDGRPLPQTDQVQTLGGGEVLRISTAQVEDTGRYTCLASSPAGDDDKEYLVRVHVPPNIAGTDEPRDITVLRNRQVTLECKSDAVPPPVITWLRNGERLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTTREFILTVNVPPNIKGGPQSLVILLNKSTVLECIAEGVPTPRITWRKDGAVLAGNHARYSILENGFLHIQSAHVTDTGRYLCMATNAAGTDRRRIDLQVHGSLVIISPSVDDTATYECTVTNGAGDDKRTVDLTVQVPPSIADEPTDFLVTKHAPAVITCTASGVPFPSIHWTKNGIRLLPRGDGYRILSSGAIElLATQLNHAGRYTCVARNAAGSAHRHVTLHVHEPPVIQPQPSELHVILNNPILLPCEATGTPSPFITWQKEGINVNTSGRNHAVLPSGGLQISRAVREDAGTYMCVAQNPAGTALGKIKLNVQVPPVISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQIAFVQPGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPTPTIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLQSPPIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLPANGGKPCQGSDLEMRNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQPWGTCSESCGKGTQTRARLCNNPPPAFGGSYCDGAETQMQVCNERNCPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCEGSDVQSDFCNSDPCPTHGNWSPWSGWGTCSRTCNGGQMRRYRTCDNPPPSNGGRACGGPDSQIQRCNTDMCPVDGSWGSWHSWSQCSASCGGGEKTRKRLCDHPVPVKGGRPCPGDTTQVTRCNVQACPGGPQRARGSVIGNINDVEFGIAFLNATITDSPNSDTRIIRAKITNVPRSLGSAMRKIVSILNPIYWTTAKEIGEAVNGFTLTNAVFKRETQVEFATGEILQMSHIARGLDSDGSLLLDIVVSGYVLQLQSPAEVTVKDYTEDYIQTGPGQLYAYSTRLFTIDGISIPYTWNHTVFYDQAQGRMPFLVETLHASSVESDYNQIEETLGFKIHASISKGDRSNQCPSGFTLDSVGPFCADEDECAAGNPCSHSCHNAMGTYYCSCPKGLTIAADGRTCQDIDECALGRHTCHAGQDCDNTIGSYRCVVRCGSGFRRTSDGLSCQDINECQESSPCHQRCFNAIGSFHCGCEPGYQLKGRKCMDVNECRQNVCRPDQHCKNTRGGYKCIDLCPNGMTKAENGTCIDIDECKDGTBQCRYNQICENTRGSYRCVCPRGYRSQGVGRPCMDIDECENTDACLHECKNTFGSYQCICPPGYQLTHNGKTCQDIDECLEQNVHCGPNRNCFNMRGSYQCIDTPCPPNYQRDPASGFCLKNCPPNDLECALSPYALEYKLVSLPFGIATNQDLIRLVAYTQDGVMHPRTTFLMVDEEQTVPFALRDENLKGVVYTTRPLREAETYRMRVRASSYSANGTIEYQTTFIVYIAVSAYPY


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 43B.

TABLE 43BComparison of the NOV43 protein sequences.NOV43a------------------------------------------------------------(SEQ ID NO:998)NOV43b------------------------------------------------------------(SEQ ID NO:1000)NOV43cMWLDRCARNPPRNHIDAPQKAPSAFRRVLQKGELISTSSAKFSAGADGSLYVVSPGGEES(SEQ ID NO:1002)NOV43d------------------------------------------------------------(SEQ ID NO:1004)NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cGEYVCTATNTAGYAKRKVQLTVYVRPRVFGDQRGLSQDKPVEISVLAGEEVTLPCEVKSLNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPPPIITWAKETQLISPFSPRHTFLPSGSMKITETRTSDSGMYLCVATNIAGNVTQAVKLNNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cVHVPPKIQRGPKHLKVQVGQRVDIPCNAQGTPLPVITWSKGGSTMLVDGEHHVSNPDGTLNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cSIDQATPSDAGIYTCVATNIAGTDETEITLHVQEPPTVEDLEPPYNTTFQERVANQRIEFNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPCPAKGTPKPTIKWLHNGRELTGREPGISILEDGTLLVIASVTPYDNGEYICVAVNEAGTNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cTERKYNLKVHVPPVIKDKEQVTNVSVLLNQLTNLFCEVEGTPSPIIMWYKDNVQVTESSTNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43cIQTVNNGKILKLFEATPEDAGRYSCKAINIAGTSQKYFNIDVLGTNFPNEVSVVLNRDVANOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43cLECQVKGTPFPDIHWFKDGNIKGGNVTTDISVLINSLIKLECETRGLPMPAITWYKDGQPNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cIMSSSQALYIDKGQYLHIPRAQVSDSATYTCHVANVAGTAEKSFHVDVYVPPMIEGNLATNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPLNKQVVIAHSLTLECKAAGNPSPILTWLKDGVPVKANDNTRIEAGGKKLEIMSAQEIDRNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cGQYICVATSVAGEKEIKYEVDVLVPPAIEGGDETSYFIVMVNNLLELDCHVTGSPPPTIMNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cWLKDGQLIDERDGFKILLNGRKLVIAQAQVSNTGLYRCMAANTAGDHKKEFEVTVHVPPTNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cIKSSGLSERVVVKYKPVALQCIANGIPNPSITWLKDDQPVNTAQGNLKIQSSGRVLQIAKNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cTLLEDAGRYTCVATNAAGETQQHIQLHVHEPPSLEDAGKMLNETVLVSNPVQLECKAAGNNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPVPVITWYKDNRNLSGSTSMTFLNRGQIIDIESAQISDAGIYKCVAINSAGATELFYSLQNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cVHVAPSISGSNNMVAVVVNNPVRLECEARGIPAPSLTWLKDGSPVSSFSNGLQVLSGGRINOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cLALTSAQISDTGRYTCVAVNAAGEKQRDIDLRVYVPPNIMGEEQNVSVLISQAVELLCQSNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cDAIPPPTLTWLKDGHPLLKKPGLSISENRSVLKIEDAQVQDTGRYTCEATNVAGKTEKNYNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cNVNIWVPPNIGGSDELTQLTVIEGNLISLLCESSGIPPPNLIWKKKGSPVLTDSMGRVRINOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cLSGGRQLQISIAEKSDAALYSCVASNVAGTAKKEYNLQVYIRPTITNSGSHPTEIIVTRGNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cKSISLECEVQGIPPPTVTWMKDGHPLIKAKGVEILDEGHILQLKNIHVSDTGRYVCVAVNNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cVAGMTDKKYDLSVHGGRMLRLMQTTMEDAGQYTCVVPNAAGEERKIFGLSVLVPPHIVGENOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cNTLEDVKVKEKQSVTLTCEVTGNPVPEITWHKDGQPLQEDEAHHIISGGRFLQITNVQVPNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cHTGRYTCLASSPAGHKSRSFSLNVFVSPTIAGVGSDGNPEDVTVILNSPTSLVCEAYSYPNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPATITWFKDGTPLESNRNIRILPGGRTLQILNAQEDNAGRYSCVATNEAGEMIKHYEVKVNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cYTLNANIVIIESQPLKSDDHVNIAANGHTLQIKEAQISDTGRYTCVASNIAGEDELDFDVNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cNIQVPPSFQKLWEIGNNLDTGRNGEAKDVIINNPISLYCETNAAPPPTLTWYKDGHPLTSNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cSDKVLILPGGRVLQIPRAKVEDAGRYTCVAVNEAGEDSLQYDVRVLVPPIIKGANSDLPENOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cEVTVLVNKSALIECLSSGSPAPRNSWQKDGQPLLEDDHHKFLSNGRILQILNTQITDIGRNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cYVCVAENTAGSAKKYFNLNVHVPPSVIGPKSENLTVVVNNFISLTCEVSGFPPPDLSWLKNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cNEQPIKLNTNTLIVPGGRTLQIIRAKVSDGGEYTCIAINQAGESKKKFSLTVYVPPSIKDNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cHDSESLSVVNVREGTSVSLECESNAVPPPVITWYKNGRMITESTHVEILADGQMLHIKKANOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cEVSDTGQYVCRAINVAGRDDKNFHLNVYVPPSIEGPEREVIVETISNPVTLTCDATGIPPNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPTIAWLKNHKRIENSDSLEVRILSGGSKLQIARSQHSDSGNYTCIASNMEGKAQKYYFLSNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cIQVPPSVAGAEIPSDVSVLLGENVELVCNANGIPTPLIQWLKDGKPIASGETERIRVSANNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cGSTLNIYGALTSDTGKYTCVATNPAGEEDRIFNLNVYVTPTIRGNKDEAEKLMTLVDTSTNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cNIECRATGTPPPQINWLKNGLPLPLSSHIRLLAAGQVIRIVRAQVSDVAVYTCVASNRAGNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cVDNKHYNLQVFAPPNMDNSMGTEEITVLKGSSTSMACITDGTPAPSMAWLRNGQPLGLDANOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cHLTVSTHGMVLQLLKAETEDSGKYTCIASNEAGEVSKHFILKVLEPPHINGSEEHEEISVNOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cIVNNPLELTCIASGIPAPKMTWMKDGRPLPQTDQVQTLGGGEVLRISTAQVEDTGRYTCLNOV43d-------------------MTWMKDGRPLPQTDQVQTLGGGEVLRISTAQVEDTGRYTCLNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cASSPAGDDDKEYLVRVHVPPNTAGTDEPRDITVLRNRQVTLECKSDAVPPPVITWLRNGENOV43dASSPAGDDDKEYLVRVHVPPNIAGTDEPRDITVLRNRQVTLECKSDAVPPPVITWLRNGENOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cRLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTTREFILTVNVPPNIKGGPQNOV43dRLQATPRVRILSGGRYLQINNADLGDTANYTCVASNIAGKTTREFILTVNVPPNIKGGPQNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cSLVILLNKSTVLECIAEGVPTPRITWRKDGAVLAGNHARYSTLENGFLHIQSAHVTDTGRNOV43dSLVILLNKSTVLECIAEGVPTPRITWRKDGAVLAGNHARYSILENGFLHIQSAHVTDTGRNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cYLCMATNAAGTDRIRIDLQVHVPPSIAPGPTNMTVIVNVQTTLACEATGIPKPSINWRKNNOV43dYLCMATNAAGTDRRRIDLQVHG--------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cGHLLNVDQNQNSYRLLSSGSLVIISPSVDDTATYECTVTNGAGDDKRTVDLTVQVPPSIANOV43d-------------------SLVIISPSVDDTATYECTVTNGAGDDKRTVDLTVQVPPSIANOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cDEPTDFLVTKHAPAVITCTASGVPFPSIHWTKNGIRLLPRGDGYRILSSGAIEILATQLNNOV43dDEPTDFLVTKHAPAVITCTASGVPFPSIHWTKNGIRLLPRGDGYRILSSGAIEILATQLNNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cHAGRYTCVARNAAGSAHRHVTLHVHEPPVIQPQPSELHVILNNPILLPCEATGTPSPFITNOV43dHAGRYTCVARNAAGSAHRHVTLHVHEPPVIQPQPSELHVILNNPILLPCEATGTPSPFITNOV43a------------------------------------MCVAQNPAGTALGKIKLNVQVPPVNOV43b---------------------------------GSTMCVAQNPAGTALGKIKLNVQVPPVNOV43cWQKEGINVNTSGRNHAVLPSGGLQISRAVREDAGTYMCVAQNPAGTALGKIKLNVQVPPVNOV43dWQKEGINVNTSGRNHAVLPSGGLQISRAVREDAGTYMCVAQNPAGTALGKIKLNVQVPPVNOV43aISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQIAFVQPNOV43bISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQIAFVQPNOV43cISPHLKEYVIAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQITFVQPNOV43dISPHLKEYVTAVDKPITLSCEADGLPPPDITWHKDGRAIVESIRQRVLSSGSLQIAFVQPNOV43aGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINOV43bGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINOV43cGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINOV43dGDAGHYTCMAANVAGSSSTSTKLTVHVPPRIRSTEGHYTVNENSQAILPCVADGIPTPAINOV43aNWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPNOV43bNWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPNOV43cNWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPNOV43dNWKKDNVLLANLLGKYTAEPYGELILENVVLEDSGFYTCVANNAAGEDTHTVSLTVHVLPNOV43aTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKNOV43bTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKNOV43cTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKNOV43dTFTELPGDVSLNKGEQLRLSCKATGIPLPKLTWTFNNNIIPAHFDSVNGHSELVIERVSKNOV43aEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPTPTNOV43bEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPTPTNOV43cEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSHWIEPLGGNAILNCEVKGDPTPTNOV43dEDSGTYVCTAENSVGFVKAIGFVYVKEPPVFKGDYPSNWIEPLGGNAILNCEVKGDPTPTNOV43aIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLQSPPNOV43bIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLRSPPNOV43cIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLQSPPNOV43dIQWNRKGVDIEISHRIRQLGNGSLAIYGTVNEDAGDYTCVATNEAGVVERSMSLTLQSPPNOV43aIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQNOV43bIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQNOV43cIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQNOV43dIITLEPVETVINAGGKIILNCQATGEPQPTITWSRQGHSISWDDRVNVLSNNSLYIADAQNOV43aKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLNOV43bKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLNOV43cKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWRACSVTCGKGIQKRSRLCNQPLNOV43dKEDTSEFECVARNLMGSVLVRVPVIVQVHGGFSQWSAWPACSVTCGKGIQKRSRLCNQPLNOV43aPANGGKPCQGSDLEMRNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHNOV43bPANGGKPCQGSDLEMRNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHNOV43cPANGGKPCQGSDLEMRNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHNOV43dPANGGKPCQGSDLEMPNCQNKPCPVDGSWSEWSLWEECTRSCGRGNQTRTRTCNNPSVQHNOV43aGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQPWGTCSESCGKGTQTRARLCNNPPPAFGGSNOV43bGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQLWGTCSESCGKGTQTRARLCNNPPPAFGGSNOV43cGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQPWGTCSESCGKGTQTRARLCNNPPPAFGGSNOV43dGGRPCEGNAVEIIMCNIRPCPVHGAWSAWQPWGTCSESCGKGTQTRARLCNNPPPAFGGSNOV43aYCDGAETQMQVCNERICPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCENOV43bYCDGAETQMQVCNERNCPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCENOV43cYCDGAETQMQVCNERNCPVHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCENOV43dYCDGAETQMQVCNERNCPIHGKWATWASWSACSVSCGGGARQRTRGCSDPVPQYGGRKCENOV43aGSDVQSDFCNSDPCPSECWKYPW-------------------------------------NOV43bGSDVQSDFCNSDPCPSECWKYPWLE-----------------------------------NOV43cGSDVQSDFCNSDPCPTHGNWSPWSGWGTCSRTCNGGQMRRYRTCDNPPPSNGGRACGGPDNOV43dGSDVQSDFCNSDPCPTHGNWSPWSGWGTCSRTCNGGQMRRYRTCDNPPPSNGGRACGGPDNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cSQIQRCNTDMCPVDGSWGSWHSWSQCSASCGGGEKTRKRLCDHPVPVKGGRPCPGDTTQVNOV43dSQIQRCNTDMCPVDGSWGSWHSWSQCSASCGGGEKTRKRLCDHPVPVKGGRPCPGDTTQVNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cTRCNVQACPGGPQRARGSVIGNINDVEFGIAFLNATITDSPNSDTRIIRAKITNVPRSLGNOV43dTRCNVQACPGGPQRARGSVIGNINDVEFGIAFLNATITDSPNSDTRIIRAKITNVPRSLGNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cSAMRKIVSILNPIYWTTAKEIGEAVNGFTLTNAVFKRETQVEFATGEILQMSHIARGLDSNOV43dSAMRKIVSILNPIYWTTAKEIGEAVNGFTLTNAVFKRETQVEFATGEILQMSHIARGLDSNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cDGSLLLDIVVSGYVLQLQSPAEVTVKDYTEDYIQTGPGQLYAYSTRLFTIDGISIPYTWNNOV43dDGSLLLDIVVSGYVLQLQSPAEVTVKDYTEDYIQTGPGQLYAYSTRLFTIDGISIPYTWNNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cHTVFYDQAQGRNPFLVETLHASSVESDYNQIEETLGFKTHASISKGDRSNQCPSGFTLDSNOV43dHTVFYDQAQGRMPFLVETLHASSVESDYNQIEETLGFKIHASISKGDRSNQCPSGFTLDSNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cVGPFCADEDECAAGNPCSHSCHNAMGTYYCSCPKGLTIAADGRTCQDIDECALGRRTCHANOV43dVGPFCADEDECAAGNPCSHSCHNAMGTYYCSCPKGLTIAADGRTCQDIDECALGRHTCHANOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cGQDCDNTIGSYRCVVRCGSGFRRTSDGLSCQDINECQESSPCHQRCFNAIGSFHCGCEPGNOV43dGQDCDNTIGSYRCVVRCGSGFRRTSDGLSCQDINECQESSPCHQRCFNAIGSFHCGCEPGNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cYQLKGRKCMDVNECRQNVCRPDQHCKNTRGGYKCIDLCPNGMTKAENGTCIDIDECKDGTNOV43dYQLKGRKCMDVNECRQNVCRPDQHCKNTRGGYKCIDLCPNGMTKAENGTCIDIDECKDGTNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cHQCRYNQICENTRGSYRCVCPRGYRSQGVGRPCMDINECEQVPKPCAHQCSNTPGSFKCINOV43dHQCRYNQICENTRGSYRCVCPRGYRSQGVGRPCMDI------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cCPPGQHLLGDGKSCAGLERLPNYGTQYSSYNLARFSPVRNNYQPQQHYRQYSHLYSSYSENOV43d------------------------------------------------------------NOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cYRNSRTSLSRTRRTIRKTCPEGSEASHDTCVDIDECENTDACQHECKNTFGSYQCICPPGNOV43d---------------------------------DECENTDACLHECKNTFGSYQCICPPGNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cYQLTHNGKTCQDIDECLEQNVHCGPNRMCFNMRGSYQCIDTPCPPNYQRDPVSGFCLKNCNOV43dYQLTHNGKTCQDIDECLEQNVHCGPNRMCFNMRGSYQCIDTPCPPNYQRDPASGFCLKNCNOV43a------------------------------------------------------------NOV43b------------------------------------------------------------NOV43cPPNDLECALSPYALEYKLVSLPFGIATNQDLIRLVAYTQDGVMHPRTTFLMVDEEQTVPFNOV43dPPNDLECALSPYALEYKLVSLPFGIATNQDLIRLVAYTQDGVMHPRTTFLMVDEEQTVPFNOV43a-------------------------------------------------------NOV43b-------------------------------------------------------NOV43cALRDENLKGVVYTTRPLREAETYRMRVRASSYSANGTIEYQTTFIVYIAVSAYPYNOV43dALRDENLKGVVYTTRPLREAETYRNRVRASSYSANGTIEYQTTFIVYIAVSAYPY


Further analysis of the NOV43a protein yielded the following properties shown in Table 43C.

TABLE 43CProtein Sequence Properties NOV43aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 13; peak value 2.20PSG score:−2.20GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −8.62possible cleavage site: between 48 and 49>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 0.63 (at 454)ALOM score: 0.63 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):4.71Hyd Moment(95):3.65G content:2D/E content:1S/T content:2Score: −5.83Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.5%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:KKXX-like motif in the C-terminus: WKYPSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):73.9%: nuclear13.0%: mitochondrial13.0%: cytoplasmic>> prediction for CG56914-01 is nuc (k = 23)


A search of the NOV43a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 43D.

TABLE 43DGeneseq Results for NOV43aNOV43aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABU12084Human NOV25c CG56914-03 protein1 . . . 707701/707(99%)0.0SEQ ID 88 - Homo sapiens, 1902 aa.441 . . . 1147 702/707(99%)[WO200281625-A2, 17-OCT-2002]ABP60991Novel human protein. SEQ ID 78 -1 . . . 707701/707(99%)0.0Homo sapiens, 5635 aa.4057 . . . 4763 702/707(99%)[WO200250105-A1, 27-JUN-2002]AAB47771Human thrombospondin protein,1 . . . 707699/707(98%)0.0BTL.012 - Homo sapiens, 1336 aa.184 . . . 890 702/707(98%)[WO200174852-A2, 11-OCT-2001]ABU12083Human NOV25b CG93858-02 protein1 . . . 707698/707(98%)0.0SEQ ID 86 - Homo sapiens, 2572 aa.1111 . . . 1817 701/707(98%)[WO200281625-A2, 17-OCT-2002]ABG03933Novel human diagnostic protein #3924 -1 . . . 471471/471(100%)0.0Homo sapiens, 1240 aa.442 . . . 912 471/471(100%)[WO200175067-A2, 11-OCT-2001]


In a BLAST search of public sequence databases, the NOV43a protein was found to have homology to the proteins shown in the BLASTP data in Table 43E.

TABLE 43EPublic BLASTP Results for NOV43aNOV43aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96RW7Hemicentin - Homo sapiens1 . . . 707700/707 (99%)0.0(Human), 5636 aa.4058 . . . 4764 701/707 (99%)Q96SC3Fibulin-6 - Homo sapiens (Human),1 . . . 707698/707 (98%)0.02673 aa (fragment).1095 . . . 1801 701/707 (98%)Q8NDA2Hypothetical protein - Homo sapiens2 . . . 489208/503 (41%) e−111(Human), 1340 aa (fragment).824 . . . 1326 285/503 (56%)Q96DN3Hypothetical protein FLJ31995 -1 . . . 460159/475 (33%)1e−63Homo sapiens (Human), 1252 aa782 . . . 1252 235/475 (49%)(fragment).Q8I0L3C. elegans him-4 protein2 . . . 511159/529 (30%)1e−59(corresponding sequence F15G9.4a) -3014 . . . 3521 241/529 (45%)Caenorhabditis elegans, 5175 aa.


PFam analysis predicts that the NOV43a protein contains the domains shown in the Table 43F.

TABLE 43FDomain Analysis of NOV43aIdentities/PfamNOV43aSimilarities forExpectDomainMatch Regionthe Matched RegionValueig37 . . . 9421/61 (34%)1.1e−0944/61 (72%)ig127 . . . 18517/62 (27%)3.2e−0638/62 (61%)ig218 . . . 27420/60 (33%)1.3e−1044/60 (73%)ig308 . . . 36521/61 (34%)5.3e−0943/61 (70%)ig398 . . . 45518/61 (30%)3.5e−0842/61 (69%)tsp_1477 . . . 52728/53 (53%)8.9e−1737/53 (70%)tsp_1534 . . . 58425/53 (47%)4.8e−1442/53 (79%)tsp_1591 . . . 64122/53 (42%)2.7e−1236/53 (68%)tsp_1648 . . . 69823/53 (43%)1.7e−1437/53 (70%)


Example 44

The NOV44 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 44A.

TABLE 44ANOV44 Sequence AnalysisNOV44a, CG56959-02SEQ ID NO: 10051599 bpDNA SequenceORF Start: ATG at 25ORF Stop: TAA at 1594GACTCACGAAAGAAAAGCCAAACGATGAGTTTCCACAAGGAGGACGGAGTGAACAGTCTGTGCCAGAAGGCTCTGCACATCGTCACCGAGCTGTGCTTCGCCGGCCAGGTGGAGTGGGAGAAGTGCTCGGGCATCTTCCCTCGGGACAGGGGCAGCCAGGGCGGAAGCAGCACAGATATTTCAGTCAGCCTGTTAGCAGTCGTTGTCAGCTTTTGTGGACTGGCCTTGTTGGTTGTCTCACTTTTTGTCTTCTGGAAGCTGTGTTGGCCATGCTGGAAAAGCAAACCTGTGACTTCCAACATCACTACCCTTCCACAGAGCATTTCAAGTGCTCCTACTGAAGTTTTTGAGACTGAAGAGAAAAAAGAAATTAAGGAAAATGAAAAGCCAGCCGTAAAAGCTATTGAGCCTGCAATAAAAATCAGCCACACTTCCCCTGACATCCCAGCAGAAGTCCAAACTGCTTTAAAAGAACATTTAATTAAACATGCACGTGTGCAAAGACAAATTACTGAGCCTACGTCATCAACCCGCCACAGTTCCTTCCGAAGACACCTGCCGAGGCAAATGCAGGTTTCCAGTGTTGATTTTAGCATGGGCACAGAACCTGTTTTACAACGAGGAGAAACAACAACCAGCATTGGGAGGATAAAGCCAGAACTCTACAAACAGAAATCAGTTGACTCTGAGGGCAACCAAAACGAAGATGTCAAAATCTGTGGGAAACTTAACTTTACCCTCCAGTATGATTATGAAAATGAACTTCTAGTTGTTAAAATTATCAAAGCTTTAGATCTCCCTGCTAAAGACTTCACAGGAACTTCTGACCCTTATGTGAAGATGTATCTTCTTCCAGATAGGAAAAAGAAATTTCAGACCCGCGTGCACAGAAAGACTTTAAATCCTCTATTTGATGAAACTTTTCAATTTCCTGTAGCATATGATCAACTAAGCAACCGAAAACTACATTTCAGTGTGTATGATTTTGACAGATTTTCTAGACATGACATGATTGGGGAAGTGATTCTTGATAATTTGTTTGAAGTCTCTGATCTCTCCAGGGAAGCCACAGTATGGAAAGATATTCACTGTGCTACCACAGAAAGTATAGACCTGGGTGAAATCATGTTTTCCCTTTGTTACCTACCGACGGCTGGGCGTATGACATTGACAGTCATTAAGTGCAGAAATCTGAAGGCGATGGATATTACTGGCTCATCAGATCCTTATGTCAAAGTGTTCCTGATGTGTGAAGGTCGAAGATTAAAAAAGAGGAAAACAACTACAAAGAAAAACACTCTAAACCCTGTGTACAATGAGGCCATTATTTTTGACATCCCTCCAGAGAACGTGGACCAGGTCAGCCTCTCCATTGCGGTCATGGATTACGATAGGGTAGGACACAATGAGGTCATAGGAGTGTGCAGAACAGGACTGGATGCTGAGGGTCTTGGGCGAGACCACTGGAATGAAATGCTGGCCTATCATCGAAAACCAATAACGCACTGGCACCCATTGCTGGAGTTACCTGGCCGGGCGACCAGTTTTGATAGTCAAGGATCCTGCCCTTCTCCTAAACCACCTTCCACACCATAATGCNOV44a, CG56959-02SEQ ID NO: 1006523 aaMW at 59186.2kDProtein SequenceMSFHKEDGVNSLCQKALHIVTELCFAGQVEWEKCSGIFPRDRGSQGGSSTDISVSLLAVVVSFCGLALLVVSLFVFWKLCWPCWKSKPVTSNITTLPQSISSAPTEVFETEEKKEIKENEKPAVKAIEPAIKISHTSPDIPAEVQTALKEHLIKHARVQRQITEPTSSTRHSSFRRHLPRQMQVSSVDFSMGTEPVLQRGETTTSIGRIKPELYKQKSVDSEGNQNEDVKICGKLNFTLQYDYENELLVVKIIKALDLPAKDFTGTSDPYVKMYLLPDRKKKFQTRVHRKTLNPLFDETFQFPVAYDQLSNRKLHFSVYDFDRFSRHDMIGEVILDNLFEVSDLSREATVWKDIHCATTESIDLGEIMFSLCYLPTAGPMTLTVIKCRNLKAMDITGSSDPYVKVFLMCEGRRLKKRKTTTKKNTLNPVYNEAIIFDIPPENVDQVSLSIAVMDYDRVGHNEVIGVCRTGLDAEGLGRDHWNEMLAYHRKPITHWHPLLELPGRATSFDSQGSCPSPKPPSTPNOV44b, CG56959-01SEQ ID NO: 10071697 bpDNA SequenceORF Start: ATG at 78ORF Stop: TAA at 1647CAGAGGGGTTAGAGGTACGGGAAGAGGAAAAGACGGCTAACTGGGAAAAAAAGAGAAAACGAAAGAAAAGCCAAACGATGAGTTTCCACAAGGAGGACGGAGTGAACAGTCTGTGCCAGAAGGCTCTGCACATCGTCACCGAGCTGTGCTTCGCCGGCCAGGTGGAGTGGGAGAAGTGCTCGGGCATCTTCCCTCGGGACAGGGGCAGCCAGGGCGGAAGCAGCACAGATATTTCAGTCAGCCTGTTAGCTGTCGTTGTCAGCTTTTGTGGACTGGCCTTGTTGGTTGTCTCACTTTTTGTCTTCTGGAAGCTGTGTTGGCCATGCTGGAAAAGCAAACCTGTGACTTCCAACATCACTACGCTTCCACAGAGCATTTCAAGTGCTCCTACTGAAGTTTTTGAGACTGAAGAGAAAAAAGAAATTAAGGAAAATGAAAAGCCAGCCGTAAAAGCTATTGAGCCTGCAATAAAAATCAGCCACACTTCCCCTGACATCCCAGCAGAAGTCCAAACTGCTTTAAAAGAACATTTAATTAAACATGCACGTGTGCAAAGACAAATTACTGAGCCTACGTCATCAACCCGGCACAGTTCCTTCCGAAGACACCTGCCGAGGCAAATGCAGGTTTCCAGTGTTGATTTTAGCATGGGCACAGAACCTGTTTTACAACGAGGAGAAACAACAACCAGCATTGGGAGGATAAAGCCAGAACTCTACAAACAGAAATCAGTTGACTCTGAGGGCAACCAAAACGAAGATGTCAAAATCTGTGGGAAACTTAACTTTACCCTCCAGTATGATTATGAAAATGAACTTCTAGTTGTTAAAATTATCAAAGCTTTAGATCTCCCTGCTAAAGACTTCACAGGAACTTCTGACCCTTATGTGAAGATGTATCTTCTTCCAGATAGGAAAAAGAAATTTCAGACCCGCGTGCACAGAAAGACTTTAAATCCTCTATTTGATGAAACTTTTCAATTTCCTGTAGCATATGATCAACTAAGCAACCGAAAACTACATTTCAGTGTGTATGATTTTGACAGATTTTCTAGACATGACATGATTGGGGAAGTGATTCTTGATAATTTGTTTGAAGTCTCTGATCTCTCCAGGGAAGCCACAGTATGGAAAGATATTCACTGTGCTACCACAGAAAGTATAGACCTGGGTGAAATCATGTTTTCCCTTTGTTACCTACCGACGGCTGGGCGTATGACATTGACAGTCATTAAGTGCAGAAATCTGAAGGCGATGGATATTACTGGCTCATCAGATCCTTATGTCAAAGTGTCCCTGATGTGTGAAGGTCGAAGATTAAAAAAGAGGAAAACAACTACAAAGAAAAACACTCTAAACCCTGTGTACAATGAGGCCATTATTTTTGACATCCCTCCAGAGAACGTGGACCAGGTCAGCCTCTCCATTGCGGTCATGGATTACGATAGGGTAGGACACAATGAGGTCATAGGAGTGTGCAGAACAGGACTGGATGCTGAGGGTCTTGGGCGAGACCACTGGAATGAAATGCTGGCCTATCATCGAAAACCAATAACGCACTGGCACCCATTGCTGGAGTTACCTGGCCGGGCGACCAGTTTTGATAGTCAAGGATCCTGCCCTTCTCCTAAACCACCTTCCACACCATAATGCCTCCAAAATGAGACCATGATATTAAGCATCTAGGATCACGTGCTCNOV44b, CG56959-01SEQ ID NO: 1008523 aaMW at 59126.1kDProtein SequenceMSFHKEDGVNSLCQKALHIVTELCFAGQVEWEKCSGIFPRDRGSQGGSSTDISVSLLAVVVSFCGLALLVVSLFVFWKLCWPCWKSKPVTSNITTLPQSISSAPTEVFETEEKKEIKENEKPAVKAIEPAIKISHTSPDIPAEVQTALKEHLIKHARVQRQITEPTSSTRHSSFRRHLPRQMQVSSVDFSMGTEPVLQRGETTTSIGRIKPELYKQKSVDSEGNQNEDVKICGKLNFTLQYDYENELLVVKIIKALDLPAKDFTGTSDPYVKMYLLPDRKKKFQTRVHRKTLNPLFDETFQFPVAYDQLSNRKLHFSVYDFDRFSRHDMIGEVILDNLFEVSDLSREATVWKDIHCATTESIDLGEIMFSLCYLPTAGRMTLTVIKCRNLKAMDITGSSDPYVKVSLMCEGRRLKKRKTTTKKNTLNPVYNEAIIFDIPPENVDQVSLSIAVMDYDRVGHNEVIGVCRTGLDAEGLGRDHWNEMLAYHRKPITHWHPLLELPGRATSFDSQGSCPSPKPPSTP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 44B.

TABLE 44BComparison of the NOV44 protein sequences.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(SEQ ID NO: 1006)NOV44b(SEQ ID NO: 1008)


Further analysis of the NOV44a protein yielded the following properties shown in Table 44C.

TABLE 44CProtein Sequence Properties NOV44aSignalP analysis:Cleavage site between residues 32 and 33PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 7; pos. chg 1; neg. chg 2H-region:length 7; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −7.65possible cleavage site: between 28 and 29>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 59-75−11.36PERIPHERALLikelihood = 4.35 (at 247)ALOM score: −11.36 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 66Charge difference: 3.0 C(3.0)-N(0.0)C > N: C-terminal side will be inside>>> membrane topology: type 1b (cytoplasmic tail 59 to 523)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):5.17Hyd Moment(95):1.90G content:0D/E content:2S/T content:1Score: −7.37Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: RKKK (5) at 279pat4: KKRK (5) at 415pat7: PDRKKKF (5) at 277bipartite: nonecontent of basic residues: 12.6%NLS Score: 0.58KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 68LL at 247LL at 275LL at 498checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):39.1%: nuclear26.1%: cytoplasmic13.0%: mitochondrial 8.7%: vesicles of secretory system 4.3%: vacuolar 4.3%: peroxisomal 4.3%: endoplasmic reticulum>> prediction for CG56959-02 is nuc (k = 23)


A search of the NOV44a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 44D.

TABLE 44DGeneseq Results for NOV44aNOV44aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABJ04645Protein of NOVX 6 SEQ ID No 12 -1 . . . 523522/523 (99%)0.0Unidentified, 523 aa. [WO200246409-1 . . . 523522/523 (99%)A2, 13-JUN-2002]AAO19183Human neurotransmission-associated1 . . . 523521/533 (97%)0.0protein NTRAN6 - Homo sapiens, 5331 . . . 533522/533 (97%)aa. [WO200266646-A2, 29-AUG-2002]ABP47935Human polypeptide SEQ ID NO 365 -78 . . . 501 296/424 (69%)e−176Homo sapiens, 461 aa.33 . . . 450 348/424 (81%)[US2002042386-A1, 11-APR-2002]AAU19715Human novel extracellular matrix78 . . . 501 296/424 (69%)e−176protein, Seq ID No 365 - Homo33 . . . 450 348/424 (81%)sapiens, 461 aa. [WO200155368-A1,02-AUG-2001]ABP69319Human polypeptide SEQ ID NO 1366 -84 . . . 501 291/418 (69%)e−172Homo sapiens, 425 aa.3 . . . 414343/418 (81%)[WO200270539-A2, 12-SEP-2002]


In a BLAST search of public sequence databases, the NOV44a protein was found to have homology to the proteins shown in the BLASTP data in Table 44E.

TABLE 44EPublic BLASTP Results for NOV44aNOV44aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ925B8Synaptotagmin 10 - Rattus1 . . . 523487/523 (93%)0.0norvegicus (Rat), 523 aa.1 . . . 523502/523 (95%)Q9R0N4Synaptotagmin X (SytX) - Mus1 . . . 523485/523 (92%)0.0musculus (Mouse), 523 aa.1 . . . 523501/523 (95%)O08625Synaptotagmin X (SytX) - Rattus1 . . . 498462/498 (92%)0.0norvegicus (Rat), 498 aa1 . . . 498477/498 (95%)(fragment).Q9R0N8Synaptotagmin VI - Mus musculus13 . . . 501 329/496 (66%)0.0(Mouse), 511 aa.12 . . . 500 391/496 (78%)Q62746Synaptotagmin VI - Rattus13 . . . 501 329/496 (66%)0.0norvegicus (Rat), 511 aa.12 . . . 500 389/496 (78%)


PFam analysis predicts that the NOV44a protein contains the domains shown in the Table 44F.

TABLE 44FDomain Analysis of NOV44aIdentities/SimilaritiesPfamNOV44afor theExpectDomainMatch RegionMatched RegionValueC2248 . . . 33444/89 (49%)6.2e−3983/89 (93%)C2380 . . . 46838/90 (42%)1.6e−3578/90 (87%)


Example 45

The NOV45 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 45A.

TABLE 45ANOV45 Sequence AnalysisNOV45a, CG57111-01SEQ ID NO: 10092855 bpDNA SequenceORF Start: at 3ORF Stop: TGA at 2844GAGGGAATGCGCGCAGCTCACAGGCCCTGGGAGTGAGCTGGTGCCCGGCGACCTGGCACCCGCGCCTGGATATGGGGCGTCTACATCGTCCCAGGAGCAGCACCAGCTACAGGAACCTGCCGCATCTGTTTCTGTTTTTCCTCTTCGTGGGACCCTTCAGCTGCCTCGGGAGTTACAGCCGGGCCACCGAGCTTCTGTACAGCCTAAACGAGGGACTACCCGCGGGGGTGCTCATCGGCAGCCTGGCCGAGGACCTGCGGCTGCTGCCCAGGTCTGCAGGGAGGCCGGACCCGCAGTCGCAGCTGCCAGAGCGCACCGGTGCTGAGTGGAACCCCCCTCTCTCCTTCAGCCTGGCCTCCCGGGGACTGAGTGGCCAGTACGTGACCCTAGACAACCGCTCTGGGGAGCTGCACACTTCAGCTCAGGAGATCGACAGGGAGGCCCTGTGTGTTGAAGGGGGTGGAGGGACTGCGTGGAGCGGCAGCGTTTCCATCTCCTCCTCTCCTTCTGACTCTTGTCTTTTGCTGCTGGATGTGCTTGTCCTGCCTCAGGAATACTTCAGGTTTGTGAAGGTGAAGATCGCCATCAGAGACATCAATGACAACGCCCCGCAGTTCCCTGTTTCCCAGATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATAGAGCATCCTGCTGTGGACCCAGATGTTGGCATTAATGGGGTTCAGACCTATCGCTTACTGGACTACCATGGTATGTTCACCCTGGACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGTGCTTTGGACAGGGAAACCCAGGACCAGTATGTGAGCATCATCACAGCTGAGGATGGTGGGTCTCCACCACTTTTGGGCAGTGCCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGACTCACAAATCAATGTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTGGATAAAGACTTGGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAGGATTTATTTCACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTGGAGTCCCACAAGCTCACCATCCTTGCTAATGGACCAGGCTGCATCCCTGCTGTAATCACTGCTCTTGTGTCCATTATTAAAGTTATTTTCAGACCCCCTGAAATTGTCCCTCGTTACATAGCAAACGAGATAGATGGTGTTGTTTATCTGAAAGAACTGGAACCCGTTAACACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATGGTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAACCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCATGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCTTAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGCTGTATGCTACAGATGCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTTAGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGATACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTGTTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTTTCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCGCCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAAGTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCCTGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTTTGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCCAGGCTCCCCGGTTACAGAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAGAGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGCTGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTCCACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCTTTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATAGGAAGGTAGAATCTGTGTCTTGTATGCCCACCTTAGTAGCTCTGTCTGTAATAAGCTTGGGTTCCATCACACTGGTCACAGGGATGGGCATATACATCTGTTTAAGGAAAGGGGAAAAGCATCCCAGGGAAGATGAAAATTTGGAAGTACAGATTCCACTGAAAGGAAAAATTGACTTGCATATGCGAGAGAGAAAGCCAATGGATATTTCTAATATTTGATATTTCATGNOV45a, CG57111-01SEQ ID NO: 1010947 aaMW at 104405.1kDProtein SequenceGNARSSQALGVSWCPATWHPRLDMGRLHRPRSSTSYRNLPHLFLFFLFVGPFSCLGSYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLASRGLSGQYVTLDNRSGELHTSAQEIDREALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFRFVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIITAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANGPGCIPAVITALVSIIKVIFRPPEIVPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSDIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGNNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVESVSCMPTLVALSVISLGSITLVTGMGIYICLRKGEKHPREDENLEVQIPLKGKIDLHMRERKPMDISNINOV45b, 277726328SEQ ID NO: 10112854 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceGGGATCCGGGAATGCGCGCAGCTCACAGGCCCTGGGAGTGAGCTGGTGCCCGGCGACCTGGCACCCGCGCCTGGATATGGGGCGTCTACATCGTCCCAGGAGCAGCACCAGCTACAGGAACCTGCCGCATCTGTTTCTGTTTTTCCTCTTCGTGGGACCCTTCAGCTGCCTCGGGAGTTACAGCCGGGCCACCGAGCTTCTGTACAGCCTAAACGAGGGACTACCCGCGGGGGTGCTCATCGGCAGCCTGGCCGAGGACCTGCGGCTGCTGCCCAGGTCTGCAGGGAGGCCGGACCCGCAGTCGCAGCTGCCAGAGCGCACCGGTGCTGAGTGGAACCCCCCTCTCTCCTTCAGCCTGGCCTCCCGGGGACTGAGTGGCCAGTACGTGACCCTAGACAACCGCTCTGGGGAGCTGCACACTTCAGCTCAGGAGATCGACAGGGAGGCCCTGTGTGTTGAAGGGGGTGGAGGGACTGCGTGGAGCGGCAGCGTTTCCATCTCCTCCTCTCCTTCTGACTCTTGTCTTTTGCTGCTGGATGTGCTTGTCCTGCCTCAGGAATACTTCAGGTTTGTGAAGGTGAAGATCGCCATCAGAGACATCAATGACAACGCCCCGCAGTTCCCTGTTTCCCAGATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATAGAGCATCCTGCTGTGGACCCAGATGTAGGCATTAATGGGGTACAGACCTATCGCTTACTGGACTACCATGGTATGTTCACCCTGGACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGTGCTTTGGACAGGGAAACCCAGGACCAGTATGTGAGCATCATCATAGCTGAGGATGGTGGGTCTCCACCACTTTTGGGCAGTGCCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGACTCACAAATCAATGTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTGGATAAAGACTTGGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAGGATTTATTTCACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTGGAGTCCCACAAGCTCACCATCCTTGCTAATGGACCAGGCTGCATCCCTGCTGTAATCACTGCTCTTGTGTCCATTATTAAAGTTATTTTCAGACCCCCTGAAATTGTCCCTCGTTACATAGCAAACGAGATAGATGGTGTTGTTTATCTGAAAGAACTGGAACCCGTTAACACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATGGTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAACCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCATGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCTTAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGCTGTATGCTACAGATGCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTTAGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGATACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTGTTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTTTCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCGCCCTCTCTGTGGTGAACCAGAGTGGTATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAAGTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCCTGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTTTGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCCAGGCTCCCCGGTTACAGAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAGAGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGCTGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTCCACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCTTTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATAGGAAGGTAGAATCTGTGTCTTGTATGCCCACCTTAGTAGCTCTGTCTGTAATAAGCTTGGGTTCCATCACACTGGTCACAGGGATGGGCATATACATCTGTTTAAGGAAAGGGGAAAAGCATCCCAGGGAAGATGAAAATTTGGAAGTACAGATTCCACTGAAAGGAAAAATTGACTTGCATATGCGAGAGAGAAAGCCAATGGATATTTCTAATATTCTCGAGNOV45b, 277726328SEQ ID NO: 1012951 aaMW at 104745.6kDProtein SequenceGSGNARSSQALGVSWCPATWHPRLDMGRLHRPRSSTSYRNLPHLFLFFLFVGPFSCLGSYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLASRGLSGQYVTLDNRSGELHTSAQEIDREALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFRFVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANGPGCIPAVITALVSIIKVIFRPPEIVPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSGIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVESVSCMPTLVALSVISLGSITLVTGMGIYICLRKGEKHPREDENLEVQIPLKGKIDLHMRERKPMDISNILENOV45c, CG57111-02SEQ ID NO: 10131020 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceAGTTACAGCCGGGCCACCGAGCTTCTGTACAGCCTAAACGAGGGACTACCCGCGGGGGTGCTCATCGGCAGCCTGGCCGAGGACCTGCGGCTGCTGCCCAGGTCTGCAGGGAGGCCGGACCCGCAGTCGCAGCTGCCAGAGCGCACCGGTGCTGAGTGGAACCCCCCTCTCTCCTTCAGCCTGGCCTCCCGGGGACTGAGTGGCCAGTACGTGACCCTAGACAACCGCTCTGGGGAGCTGCACACTTCAGCTCAGGAGATCGACAGGGAGGCCCTGTGTGTTGAAGGGGGTGGAGGGACTGCGTGGAGCGGCAGCGTTTCCATCTCCTCCTCTCCTTCTGACTCTTGTCTTTTGCTGCTGGATGTGCTTGTCCTGCCTCAGGAATACTTCAGGTTTGTGAAGGTGAAGATCGCCATCAGAGACATCAATGACAACGCCCCGCAGTTCCCTGTTTCCCAGATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATAGAGCATCCTGCTGTGGACCCAGATGTAGGCATTAATGGGGTGCAGACCTATCGCTTACTGGACTACCATGGTATGTTCACCCTGGACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGTGCTTTGGACAGGGAAACCCAGGACCAGTATGTGAGCATCATCATAGCTGAGGATGGTGGGTCTCCACCACTTTTGGGCAGTGCCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGACTCACAAATCAATGTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTGGATAAAGACTTGGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAGGATTTATTTCACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTGGAGTCCCACAAGCTCACCATCCTTGCTNOV45c, CG57111-02SEQ ID NO: 1014340 aaMW at 36598.6kDProtein SequenceSYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLASRGLSGQYVTLDNRSGELHTSAQEIDREALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFRFVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANOV45d, CG57111-03SEQ ID NO: 10151032 bpDNA SequenceORF Start: at 7ORF Stop: at 1027GGATCCAGTTACAGCCGGGCCACCGAGCTTCTGTACAGCCTAAACGAGGGACTACCCGCGGGGGTGCTCATCGGCAGCCTGGCCGAGGACCTGCGGCTGCTGCCCAGGTCTGCAGGGAGGCCGGACCCGCAGTCGCAGCTGCCAGAGCGCACCGGTGCTGAGTGGAACCCCCCTCTCTCCTTCAGCCTGGCCTCCCGGGGACTGAGTGGCCAGTACGTGACCCTAGACAACCGCTCTGGGGAGCTGCACACTTCAGCTCAGGAGATCGACAGGGAGGCCCTGTGTGTTGAAGGGGGTGGAGGGACTGCGTGGAGCGGCAGCGTTTCCATCTCCTCCTCTCCTTCTGACTCTTGTCTTTTGCTGCTGGATGTGCTTGTCCTGCCTCAGGAATACTTCAGGTTTGTGAAGGTGAAGATCGCCATCAGAGACATCAATGACAACGCCCCGCAGTTCCCTGTTTCCCAGATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATAGAGCATCCTGCTGTGGACCCAGATGTAGGCATTAATGGGGTGCAGACCTATCGCTTACTGGACTACCATGGTATGTTCACCCTGGACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGTGCTTTGGACAGGGAAACCCAGGACCAGTATGTGAGCATCATCATAGCTGAGGATGGTGGGTCTCCACCACTTTTGGGCAGTGCCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGACTCACAAATCAATGTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTGGATAAAGACTTGGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAGGATTTATTTCACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTGGAGTCCCACAAGCTCACCATCCTTGCTCTCGAGNOV45d, CG57111-03SEQ ID NO: 1016340 aaMW at 36598.6kDProtein SequenceSYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLASRGLSGQYVTLDNRSGELHTSAQEIDREALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFRFVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANOV45e, CG57111-04SEQ ID NO: 10171390 bpDNA SequenceORF Start: at 8ORF Stop: at 1385GGGATCCCCTGAAATTGTCCCTCGTTACATAGCAAACGAGATAGATGGTGTTGTTTATCTGAAAGAACTGGAACCCGTTAACACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATGGTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAACCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCATGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCTTAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGCTGTATGCTACAGATGCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTTAGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGATACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTGTTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTTTCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCGCCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAAGTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCCTGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTTTGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCCAGGCTCCCCGGTTACAGAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAGAGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGCTGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTCCACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCTTTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATAGGAAGGTAGAACTCGAGNOV45e, CG57111-04SEQ ID NO: 1018459 aaMW at 51489.5kDProtein SequencePEIVPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSDIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVE


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 45B.

TABLE 45BComparison of the NOV45 protein sequences.NOV45a--GNARSSQALGVSWCPATWHPRLDMGRLHRPRSSTSYRNLPHLFLFFLFVGPFSCLGSYNOV45bGSGNARSSQALGVSWCPATWHPRLDMGRLHRPRSSTSYRNLPHLFLFFLFVGPFSCLGSYNOV45c----------------------------------------------------------SYNOV45d----------------------------------------------------------SYNOV45e-------------------------------------------PEIVPRYIANEIDGVVYNOV45aSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLANOV45bSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLANOV45cSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLANOV45dSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLANOV45eLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTKPMDYELQNOV45aSRGLSGQYVTLDN---------------------------------RSGELHTSAQEIDRNOV45bSRGLSGQYVTLDN---------------------------------RSGELHTSAQEIDRNOV45cSRGLSGQYVTLDN---------------------------------RSGELHTSAQEIDRNOV45dSRGLSGQYVTLDN---------------------------------RSGELHTSAQEIDRNOV45eQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATNOV45aEALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFR------------------NOV45bEALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFR------------------NOV45cEALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFR------------------NOV45dEALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFR------------------NOV45eDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGKPPRENOV45a-FVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLNOV45b-FVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLNOV45c-FVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLNOV45d-FVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLATEHPAVDPDVGINGVQTYRLLNOV45eSVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVIS-VTDADAGRNGWVALSVVNOV45aDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIITAEDGGSPPLLGSATLTIGINOV45bDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGINOV45cDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGINOV45dDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGINOV45eNQSDIFVIDTGKG-------MLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLNOV45aSDINDNCPLFTDSQIN---VTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASNOV45bSDINDNCPLFTDSQIN---VTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASNOV45cSDINDNCPLFTDSQIN---VTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASNOV45dSDINDNCPLFTDSQIN---VTVYGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASNOV45eLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRR-GPRNOV45aKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANGPGCIPAVITALVSIIKVIFRPPEIVNOV45bKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANGPGCIPAVITALVSIIKVIFRPPEIVNOV45cKDLFHLDENTGVIKLFSKIGGSVLESHKLTILA---------------------------NOV45dKDLFHLDENTGVIKLFSKIGGSVLESHKLTILA---------------------------NOV45ePESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESNOV45aPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYNOV45bPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45eYIESLLRKEPEINIEEKEPQISIEPTHRKVE-----------------------------NOV45aLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNOV45bLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYNOV45bNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADNOV45bTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aAGRNGWVALSVVNQSDIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKNOV45bAGRNGWVALSVVNQSGIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRNOV45bITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVNOV45bRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVESVSCMPTLVALSVISLGSITLVTGMNOV45bSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVESVSCMPTLVALSVISLGSITLVTGMNOV45c------------------------------------------------------------NOV45d------------------------------------------------------------NOV45e------------------------------------------------------------NOV45aGIYICLRKGEKHPREDENLEVQIPLKGKIDLHMRERKPMDISNI--NOV45bGIYICLRKGEKHPREDENLEVQIPLKGKIDLHMRERKPMDISNILENOV45c----------------------------------------------NOV45d----------------------------------------------NOV45e----------------------------------------------NOV45a(SEQ ID NO: 1010)NOV45b(SEQ ID NO: 1012)NOV45c(SEQ ID NO: 1014)NOV45d(SEQ ID NO: 1016)NOV45e(SEQ ID NO: 1018)


Further analysis of the NOV45a protein yielded the following properties shown in Table 45C.

TABLE 45CProtein Sequence Properties NOV45aSignalP analysis:Cleavage site between residues 57 and 58PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 4; pos. chg 1; neg. chg 0H-region:length 16; peak value 6.44PSG score:2.04GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.42possible cleavage site: between 56 and 57>>> Seems to have a cleavable signal peptide (1 to 56)ALOM:Klein et al's method for TM region allocationInit position for calculation: 57Tentative number of TMS(s) for the threshold 0.5: 2INTEGRALLikelihood =Transmembrane 401-417−7.38INTEGRALLikelihood =Transmembrane 886-902−3.77PERIPHERALLikelihood = 2.01 (at 173)ALOM score: −7.38 (number of TMSs: 2)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 28Charge difference: 0.5 C(3.5)-N(3.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptide>>> membrane topology: type 3bMITDISC:discrimination of mitochondrial targeting seqR content:2Hyd Moment(75):3.38Hyd Moment(95):4.41G content:2D/E content:1S/T content:4Score: −3.49Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 70 SRA|TENUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.6%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: NARSnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: GNARSSPrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: endoplasmic reticulum11.1%: Golgi11.1%: vacuolar11.1%: mitochondrial11.1%: nuclear11.1%: cytoplasmic>> prediction for CG57111-01 is end (k = 9)


A search of the NOV45a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 45D.

TABLE 45DGeneseq Results for NOV45aNOV45aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABP70119Human NOV32a - Homo sapiens, 9471 . . . 947 947/947 (100%)0.0aa. [WO200272771-A2, 19-SEP-2002]1 . . . 947 947/947 (100%)ABU52605Human NOVX protein, NOV16a -1 . . . 947946/947 (99%)0.0Homo sapiens, 962 aa.16 . . . 962 946/947 (99%)[WO200281518-A2, 17-OCT-2002]AAU09958Human cadherin-1 (CDHN-1) protein -24 . . . 947 923/924 (99%)0.0Homo sapiens, 924 aa.1 . . . 924923/924 (99%)[WO200179293-A2, 25-OCT-2001]ABP47865Human polypeptide SEQ ID NO 295 -551 . . . 947 396/397 (99%)0.0Homo sapiens, 397 aa.1 . . . 397397/397 (99%)[US2002042386-A1, 11-APR-2002]AAU19645Human novel extracellular matrix551 . . . 947 396/397 (99%)0.0protein, Seq ID No 295 - Homo1 . . . 397397/397 (99%)sapiens, 397 aa. [WO200155368-A1,02-AUG-2001]


In a BLAST search of public sequence databases, the NOV45a protein was found to have homology to the proteins shown in the BLASTP data in Table 45E.

TABLE 45EPublic BLASTP Results for NOV45a NOV45aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9NRT9Protocadherin 13 - Homo sapiens1 . . . 947 947/947 (100%)0.0(Human), 947 aa (fragment).1 . . . 947 947/947 (100%)Q8NDN4Hypothetical protein - Homo1 . . . 947946/947 (99%)0.0sapiens (Human), 951 aa.5 . . . 951946/947 (99%)Q8N6Y1Protocadherin 20 - Homo sapiens24 . . . 947 923/924 (99%)0.0(Human), 924 aa.1 . . . 924923/924 (99%)Q8BIV2PROTOCADHERIN 13 homolog -1 . . . 947874/948 (92%)0.0Mus musculus (Mouse), 952 aa.5 . . . 952910/948 (95%)Q8BIZ0PROTOCADHERIN 13 homolog -24 . . . 947 860/925 (92%)0.0Mus musculus (Mouse), 925 aa.1 . . . 925896/925 (95%)


PFam analysis predicts that the NOV45a protein contains the domains shown in the Table 45F.

TABLE 45FDomain Analysis of NOV45aIdentities/NOV45aSimilarities forExpectPfam DomainMatch Regionthe Matched RegionValuecadherin210 . . . 30736/111 (32%)2.5e−0872/111 (65%)cadherin321 . . . 41327/110 (25%)0.000466/110 (60%)cadherin536 . . . 62633/107 (31%)6.4e−1969/107 (64%)cadherin640 . . . 72939/107 (36%)5.8e−2069/107 (64%)cadherin747 . . . 84038/112 (34%)  4e−1170/112 (62%)


Example 46

The NOV46 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 46A.

TABLE 46ANOV46 Sequence AnalysisNOV46a, CG57409-05SEQ ID NO: 10191500 bpDNA SequenceORF Start: ATG at 12ORE Stop: TGA at 1476TGAGCCGAGACATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCGCCAGATCCGCTGGCCCCCAGTCCTGGCTCTGAGGACCCTGTCCTCTGGTCCCAAGCAGGGTATCCTCTGCAGAGCCCCACACCTCAGTTCTGACTTGGTTTCCCCGCTTGCTTTCTCAGCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCCCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGTGGTGGTGATGCCGGGCAGTGGAGCCCCCTGCCAGTCCAGCCCACAGCTGTGGGGGCCCATGGCCATCTTCCTCTTGGCGTTGCAGAGATGATGAGAGCTGTGTGGCCACCCCCNOV46a, CG57409-05SEQ ID NO: 1020488 aaMW at 54357.1kDProtein SequenceMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTERQIRWPPVLALRTLSSGPKQGILCRAPHLSSDLVSPLAFSAINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNPLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPGSGAPCQSSPQLWGPMAIFLLALQRNOV46b, 277731446SEQ ID NO: 10211363 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGTACCATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGTGGTGGTGATGCCGGGCAGTGGAGCCCCCTGCCAGTCCAGCCCACAGCTGTGGGGGCCCATGGCCATCTTCCTCTTGGCGTTGCAGAGACTCGAGGGCNOV46b, 277731446SEQ ID NO: 1022454 aaMW at 50606.7kDProtein SequenceTGTMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPGSGAPCQSSPQLWGPMAIFLLALQRLEGNOV46c, CG57409-07SEQ ID NO: 10231363 bpDNA SequenceORF Start: ATG at 11ORF Stop: at 1355CACCGGTACCATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGTGGTGGTGATGCCGGGCAGTGGAGCCCCCTGCCAGTCCAGCCCACAGCTGTGGGGGCCCATGGCCATCTTCCTCTTGGCGTTGCAGAGACTCGAGGGCNOV46c, CG57409-07SEQ ID NO: 1024448 aaMW at 50048.1kDProtein SequenceMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPGSGAPCQSSPQLWGPMAIFLLALQRNOV46d, 312102874SEQ ID NO: 10251369 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTCCCACCATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGTGGTGGTGATGCCGGGCAGTGGAGCCCCCTGCCAGTCCAGCCCACAGCTGTGGGGGCCCATGGCCATCTTCCTCTTGGCGTTGCAGAGAGAATTCGGCNOV46d, 312102874SEQ ID NO: 1026456 aaMW at 50922.1kDProtein SequenceTKLPTMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKATPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPGSGAPCQSSPQLWGPMAIFLLALQREFGNOV46e, 312102899SEQ ID NO: 10271249 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAAGCTTAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGGAATTCGGCNOV46e, 312102899SEQ ID NO: 1028416 aaMW at 46644.0kDProtein SequenceTKLSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPREFGNOV46f, CG57409-01SEQ ID NO: 10294141 bpDNA SequenceORF Start: ATG at 135ORF Stop: at 4140TCTTCGTCGCCGCTCTCTCTCTCACCTCTCAGGGAAAGGGGGGGACATAGGGGCGTCGCGGGGCCCCCGCGAATGCGCCCCCCGCCGCCTCTCGGGCTGCGCCGCCTCGCGGGGATGAAGCACCGGCCGTGAAGATGGAGGTGACCTGCCTTCTACTTCTGGCGCTGATCCCCTTCCACTGCCGGGGACAAGGAGTCTACGCTCCAGCCCAGGCGCAGATCGTGCATGCGGGCCAGGCATGTGTGGTGAAAGAGGACAATATCAGCGAGCGTGTCTACACCATCCGGGAGGGGGACACCCTCATGCTGCAGTGCCTTGTAACAGGGCACCCTCGACCCCAGGTACGGTGGACCAAGACGGCAGGTAGCGCCTCGGACAAGTTCCAGGAGACATCGGTGTTCAACGAGACGCTGCGCATCGAGCGTATTGCACGCACGCAGGGCGGCCGCTACTACTGCAAGGCTGAGAACGGCGTGGGGGTGCCGGCCATCAAGTCCATCCGCGTGGACGTGCAGTACCTGGATGAGCCAATGCTGACGGTGCACCAGACGGTGAGCGATGTGCGAGGCAACTTCTACCAGGAGAAGACGGTGTTCCTGCGCTGTACTGTCAACTCCAACCCGCCTGCCCGCTTCATCTGGAAGCGGGGTTCCGATACCCTATCCCACAGCCAGGACAATGGGGTTGACATCTATGAGCCCCTCTACACTCAGGGGGAGACCAAGGTCCTGAAGCTGAAGAACCTGCGGCCCCAGGACTATGCCAGCTACACCTGCCAGGTGTCTGTGCGTAACGTGTGCGGCATCCCAGACAAGGCCATCACCTTCCGGCTCACCAACACCACGGCACCACCAGCCCTGAAGCTGTCTGTGAACGAAACTCTGGTGGTGAACCCTGGGGAGAATGTGACGGTGCAGTGTCTGCTGACAGGCGGTGATCCCCTCCCCCAGCTGCAGTGGTCCCATGGGCCTGGCCCACTGCCCCTGGGTGCTCTGGCCCAGGGTGGCACCCTCAGCATCCCTTCAGTGCAGGCCCGGGACTCTGGCTACTACAACTGCACAGCCACCAACAATGTGGGCAACCCTGCCAAGAAGACTGTCAACCTGCTGGTGCGATCCATGAAGAACGCTACATTCCAGATCACTCCTGACGTGATCAAAGAGAGTGAGAACATCCAGCTGGGCCAGGACCTGAAGCTATCGTGCCACGTGGATGCAGTGCCCCAGGAGAAGGTGACCTACCAGTGGTTCAAGAATGGCAAGCCGGCACGCATGTCCAAGCGGCTGCTGGTGACCCGCAATGATCCTGAGCTGCCCGCAGTCACCAGCAGCCTAGAGCTCATTGACCTGCACTTCAGTGACTATGGCACCTACCTGTGCATGGCTTCTTTCCCAGGGGCACCCGTGCCCGACCTCAGCGTCGAGGTCAACATCTCCTCTGAGACAGTGCCGCCCACCATCAGTGTGCCCAAGGGTAGGGCCGTGGTGACCGTGCGCGAGGGATCGCCTGCCGAGCTGCAATGCGAGGTGCGGGGCAAGCCGCGGCCGCCAGTGCTCTGGTCCCGCGTGGACAAGGAGGCTGCACTGCTGCCCTCGGGGCTGCCCCTGGAGGAGACTCCGGACGGGAAGCTGCGGCTGGAGCGAGTGAGCCGAGACATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCGCCAGATCCGCTGGCCCCCAGTCCTGGCTCTGAGGACCCTGTCCTCTGGTCCCAAGCAGGGTATCCTCTGCAGAGCCCCACACCTCAGTTCTGACTTGGTTTCCCCGCTTGCTTTCTCAGCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGGTGCAAGACGGGAAGGAGCTGCCTGCGATGGCCTGAAATTCCACCTTTCATCCCCTATGGATGACGGAGAGCTTACAGATGACCCTATTGAATGCAAGCACCTTTGGATCCATAGAGTGGACAGTAAAGGTGCTCAGTACATGTTGGCTGAGCTGAACTGCATACATGTGGCCCCCAGGTTCCTGGTCTTTATGGACGAAGGGCACAAGGTTGGTGAAAAGGACTCCGGGGGCCAGCCCTTCCAAGTTTACACTGATTTCTCCTTTTACCCTCATGCTATCCCTGAGAAGATGTCAATAATGCCCACGTTACAGGTGGGAAAACTGAGGCTTAGAGAGGAGGAGGAATCTGCCTACGGTCACACAGCTGCAAAGGCTAGAGCTGGGACCAGGAGCTGGTCTCTTAACCGACCACCTGAGCTCAAGAGCTTTTCTCTCTGGACCAACATGACCCAAAGTGTGCGCGAGCCTATCACAGGTCCCCTGCAATGCCAAACATACACGCACAGCAATACACAACACCTGGGGACATGGATGAAGCTGGAAACCATCATTCTCAGCAAACTGACACAAGAACAGAAAACCAAACACCACATGTTCTCACTCACCACCCAGTCTGCCCCGCCCTCTCTCTTCTCACCTGAACTTCCCCTCTCCTCAAACTCTCGAGGCCACGCCTCTATGTCCTTGGATGATGATGATGACGACGACGACGATGATGATGATGATGATGACGACGATGACAATGATGATGATGATGGAAGGAAGACCTACAGAATCCCTCCAGGCTCTGACCTCAGTGCTTGTGGGTGGGTGAATGACCACATGTCGCAGGGAGACTCCACAGGTCCTCCCGATGAGAAGCACTCTTATGCCAAAGAGGAGACTCAGGCCAAACTGACAGGACCAGGAATTAGCTACCCTGGTAAACCCAGCTATCGACTGCACCCGAGCGGCTACACACCACTGGAGCAGTTCAGGGAGAAAGCCACCGGCATGCTCACCCCGTATGTCTCTGGCTCTGTTTCCTCTTTCTGCTTCCCCTTCCCCACCTCTGAGTCTCTGTGTTCTGCTCATGCCAATTCCCCTTCTGCCTGTCTCTGCCCGCTTCTNOV46f, CG57409-01SEQ ID NO: 10301335 aaMW at 147969.4kDProtein SequenceMEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIREGDTLMLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPMLTVHQTVSDVRGNFYQEKTVFLRCTVNSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFRLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLQWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSMKNATFQITPDVIKESENIQLGQDLKLSCHVDAVPQEKVTYQWFKNGKPARMSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGAPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLPLEETPDGKLRLERVSRDMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTERQIRWPPVLALRTLSSGPKQGILCRAPHLSSDLVSPLAFSAINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKGARREGAACDGLKFHLSSPMDDGELTDDPIECKHLWIHRVDSKGAQYMLAELNCIHVAPRFLVFMDEGHKVGEKDSGGQPFQVYTDFSFYPHAIPEKMSIMPTLQVGKLRLREEEESAYGHTAAKARAGTRSWSLNRPPELKSFSLWTNMTQSVREPITGPLQCQTYTHSNTQHLGTWNKLETIILSKLTQEQKTKHHMFSLTTQSAPPSLFSPELPLSSNSRGHASMSLDDDDDDDDDDDDDDDDDDNDDDDGRKTYRIPPGSDLSACGWVNDHMSQGDSTGPPDEKHSYAKEETQAKLTGPGISYPGKPSYRLHPSGYTPLEQFREKATGMLTPYVSGSVSSFCFPFPTSESLCSAHANSPSACLCPLNOV46g, CG57409-02SEQ ID NO: 1031720 bpDNA SequenceORF Start: at 7ORF Stop: at 715GGTACCTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCTGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGGAATTCNOV46g, CG57409-02SEQ ID NO: 1032236 aaMW at 26563.7kDProtein SequenceLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNACAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRNOV46h, CG57409-03SEQ ID NO: 10334169 bpDNA SequenceORF Start: ATG at 135ORF Stop: TGA at 4080TCTTCGTCGCCGCTCTCTCTCTCACCTCTCAGGGAAAGGGGGGGACATAGGGGCGTCGCGGGGCCCCGGCGAATGCGCCCCCCGCCGCCTCTCGGGCTGCGCCGCCTCGCGGGGATGAAGCACCGGCCGTGAAGATGGAGGTGACCTGCCTTCTACTTCTGGCGCTGATCCCCTTCCACTGCCGGGGACAAGGAGTCTACGCTCCAGCCCAGGCGCAGATCGTGCATGCGGGCCAGGCATGTGTGGTGAAAGAGGACAATATCAGCGAGCGTGTCTACACCATCCGGGAGGGGGACACCCTCATGCTGCAGTGCCTTGTAACAGGGCACCCTCGACCCCAGGTACGGTGGACCAAGACGGCAGGTAGCGCCTCGGACAAGTTCCAGGAGACATCGGTGTTCAACGAGACGCTGCGCATCGAGCGTATTGCACGCACGCAGGGCGGCCGCTACTACTGCAAGGCTGAGAACGGCGTGGGGGTGCCGGCCATCAAGTCCATCCGCGTGGACGTGCAGTACCTGGATGAGCCAATGCTGACGGTGCACCAGACGGTGAGCGATGTGCGAGGCAACTTCTACCAGGAGAAGACGGTGTTCCTGCGCTGTACTGTCAACTCCAACCCGCCTGCCCGCTTCATCTGGAAGCGGGGTTCCGATACCCTATCCCACAGCCAGGACAATGGGGTTGACATCTATGAGCCCCTCTACACTCAGGGGGAGACCAAGGTCCTGAAGCTGAAGAACCTGCGGCCCCAGGACTATGCCAGCTACACCTGCCAGGTGTCTGTGCGTAACGTGTGCGGCATCCCAGACAAGGCCATCACCTTCCGGCTCACCAACACCACGGCACCACCAGCCCTGAAGCTGTCTGTGAACGAAACTCTGGTGGTGAACCCTGGGGAGAATGTGACGGTGCAGTGTCTGCTGACAGGCGGTGATCCCCTCCCCCAGCTGCAGTGGTCCCATGGGCCTGGCCCACTGCCCCTGGGTGCTCTGGCCCAGGGTGGCACCCTCAGCATCCCTTCAGTGCAGGCCCGGGACTCTGGCTACTACAACTGCACAGCCACCAACAATGTGGGCAACCCTGCCAAGAAGACTGTCAACCTGCTGGTGCGATCCATGAAGAACGCTACATTCCAGATCACTCCTGACGTGATCAAAGAGAGTGAGAACATCCAGCTGGGCCAGGACCTGAAGCTATCGTGCCACGTGGATGCAGTGCCCCAGGAGAAGGTGACCTACCAGTGGTTCAAGAATGGCAAGCCGGCACGCATGTCCAAGCGGCTGCTGGTGACCCGCAATGATCCTGAGCTGCCCGCAGTCACCAGCAGCCTAGAGCTCATTGACCTGCACTTCAGTGACTATGGCACCTACCTGTGCATGGCTTCTTTCCCAGGGGCACCCGTGCCCGACCTCAGCGTCGAGGTCAACATCTCCTCTGAGACAGTGCCGCCCACCATCAGTGTGCCCAAGGGTAGGGCCGTGGTGACCGTGCGCGAGGGATCGCCTGCCGAGCTGCAATGCGAGGTGCGGGGCAAGCCGCGGCCGCCAGTGCTCTGGTCCCGCGTGGACAAGGAGGCTGCACTGCTGCCCTCGGGGCTGCCCCTGGAGGAGACTCCGGACGGGAAGCTGCGGCTGGAGCGAGTGAGCCGAGACATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGGTGCAAGACGGGAAGGAGCTGCCTGCGATGGCCTGAAATTCCACCTTTCATCCCCTATGGATGACGGAGAGCTTACAGATGACCCTATTGAATGCAAGCACCTTTGGATCCATAGAGTGGACAGTAAAGGTGCTCAGTACATGTTGGCTGAGCTGAACTGCATACATGTGGCCCCCAGGTTCCTGGTCTTTATGGACGAAGGGCACAAGGTTGGTGAAAAGGACTCCGGGGGCCAGCCCTTCCAAGTTTACACTGATTTCTCCTTTTACCCTCATGCTATCCCTGAGAAGATGTCAATAATGCCCACGTTACAGGTGGGAAAACTGAGGCTTAGAGAGGAGGAGGAATCTGCCTACGGTCACACAGCTGCAAAGGCTAGAGCTGGGACCAGGAGCTGGTCTCTTAACCGACCACCTGAGCTCAAGAGCTTTTCTCTCTGGACCAACATGACCCAAAGTGTGCGCGAGCCTATCACAGGTCCCCTGCAATGCCAAACATACACGCACAGCAATACACAACACCTGGGGACATGGATGAAGCTGGAAACCATCATTCTCAGCAAACTGACACAAGAACAGAAAACCAAACACCACATGTTCTCACTCACCACCCAGTCTGCCCCGCCCTCTCTCTTCTCACCTGAACTTCCCCTCTCCTCAAACTCTCGAGGCCACGCCTCTATGTCCTTGGATGATGATGATGACGACGACGACGATGATGATGATGATGATGACGACGATGACAATGATGATGATGATGGAAGGAAGACCTACAGAATCCCTCCAGGCTCTGACCTCAGTGCTTGTGGGTGGGTGAATGACCACATGTCGCAGGGAGACTCCACAGGTCCTCCCGATGAGAAGCACTCTTATGCCAAAGAGGAGACTCAGGCCAAACTGACAGGACCAGGAATTAGCTACCCTGGTAAACCCAGCTATCGACTGCACCCGAGCGGCTACACACCACTGGAGCAGTTCAGGGAGAAAGCCACCGGCATGCTCACCCCGTATGTCTCTGGCTCTGTTTCCTCTTTCTGCTTCCCCTTCCCCACCTCTGAGTCTCTGTGTTCTGCTCATGCCAATTCCCCTTCTGCCTGTCTCTGCCCGCTTCTCTCTGGGCTGGTCTCTCCGAGACTCTGTTCCCTTGGCTGGCATGCCCTCCACCTCCCCTGATGGTTCAGCAGAGATGAAGCCGGCCTGGCTCATGGGTGTGGGTAATGTACTAGTGCAGGAGAGTGGTGGGGCCCAGTCTGGGTGCAGNOV46h, CG57409-03SEQ ID NO: 10341315 aa MW at 145782.9kDProtein SequenceMEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIREGDTLMLQCLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKSIRVDVQYLDEPMLTVHQTVSDVRGNFYQEKTVFLRCTVNSNPPARFIWKRGSDTLSHSQDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFRLTNTTAPPALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLQWSHGPGPLPLGALAQGGTLSIPSVQARDSGYYNCTATNNVGNPAKKTVNLLVRSMKNATFQITPDVIKESENIQLGQDLKLSCHVDAVPQEKVTYQWFKNGKPARMSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPGAPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKEAALLPSGLPLEETPDGKLRLERVSRDMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKGARREGAACDGLKFHLSSPMDDGELTDDPIECKHLWIHRVDSKGAQYMLAELNCIHVAPRFLVFMDEGHKVGEKDSGGQPFQVYTDFSFYPHAIPEKMSIMPTLQVGKLRLREEEESAYGHTAAKARAGTRSWSLNRPPELKSFSLWTNMTQSVREPITGPLQCQTYTHSNTQHLGTWMKLETIILSKLTQEQKTKHHNFSLTTQSAPPSLFSPELPLSSNSRGHASMSLDDDDDDDDDDDDDDDDDDNDDDDGRKTYRIPPGSDLSACGWVNDHMSQGDSTGPPDEKHSYAKEETQAKLTGPGISYPGKPSYRLHPSGYTPLEQFREKATGMLTPYVSGSVSSFCFPFPTSESLCSAHANSPSACLCPLLSGLVSPRLCSLGWHALHLPNOV46i, CG57409-04SEQ ID NO: 1035720 bpDNA SequenceORF Start: at 7ORF Stop: at 715GGATCCTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCTGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGCTCGAGNOV46i, CG57409-04SEQ ID NO: 1036236 aaMW at 26563.7kDProtein SequenceLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNACAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRNOV46j, CG57409-06SEQ ID NO: 10371828 bpDNA SequenceORF Start: ATG at 12ORF Stop: TGA at 1521TGAGCCGAGACATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCGCCAGATCCGCTGGCCCCCAGTCCTGGCTCTGAGGACCCTGTCCTCTGGTCCCAAGCAGGGTATCCTCTGCAGAGCCCCACACCTCAGTTCTGACTTGGTTTCCCCGCTTGCTTTCTCAGCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCCCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGGTGCAAGACGGGAAGGAGGTGGGGGAGCTGAATCTGGAGGGAGCTGTGCGTGGCGGGGGTTCCTGTCTGTTGAGGGAGGGTGTTCGGGTCTGAATAGGGGTTCAGACTGTCTGATGATGGGAATCAGGTGGCTCTGACTGTGTTAACGTGTGCCCACAACTCACGTCAGGCTGAGAACTGGTGTAACACCATGAGAAAGCTTGGCCCCCACCATCGTGATGAGCATACCGACCTGGTCACCGGAACACAAACACCAACAACCACAGAGGGCGCCTCAGAATACCCAGAGGGCCCAATACGCCGACCCGCTGTCACGAGCGCCCACGAGCGGCAGAACACGACAGGCACACAACCAGCCGGAGCAAGACGGAGCCGAGAGCCCCGGGGACATAGACCCCAGCAAGCGACACACAAGGACGCGCACAGAGCGCACACACTAACANOV46j, CG57409-06SEQ ID NO: 1038503 aaMW at 55764.4kDProtein SequenceMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTERQIRWPPVLALRTLSSGPKQGILCRAPHLSSDLVSPLAFSAINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNPLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKGARREGGGGAESGGSCAWRGFLSVEGGCSGLNRGSDCLNMGIRWLNOV46k, CG57409-08SEQ ID NO: 10391369 bpDNA SequenceORF Start: ATG at 17ORF Stop: end of sequenceCACCAAGCTTCCCACCATGAGCGGGACCTACCGCTGCCAGACGGCCCGCTATAATGGCTTCAACGTGCGCCCCCGTGAGGCCCAGGTGCAGCTGAACGTGCAGTTCCCGCCGGAGGTGGAGCCCAGTTCCCAGGACGTGCGCCAGGCGCTGGGCCGGCCCGTGCTCCTGCGCTGCTCGCTGCTGCGAGGCAGCCCCCAGCGCATCGCCTCGGCTGTGTGGCGTTTCAAAGGGCAGCTGCTGCCGCCGCCGCCTGTTGTTCCCGCCGCCGCCGAGGCGCCGGATCACGCGGAGCTGCGCCTCGACGCCGTAACTCGCGACAGCAGCGGCAGCTACGAGTGCAGCGTCTCCAACGATGTGGGCTCGGCTGCCTGCCTCTTCCAGGTCTCCGCCAAAGCCTACAGCCCGGAGTTTTACTTCGACACCCCCAACCCCACCCGCAGCCACAAGCTGTCCAAGAACTACTCCTACGTGCTGCAGTGGACTCAGAGGGAGCCCGACGCTGTCGACCCTGTGCTCAACTACAGACTCAGCATCCGCCAGTTGAACCAGCACAATGCGGTGGTCAAGGCCATCCCGGTCCGGCGTGTGGAGAAGGGGCAGCTGCTGGAGTACATCCTGACCGATCTCCGTGTGCCCCACAGCTATGAGGTCCGCCTCACACCCTATACCACCTTCGGGGCTGGTGACATGGCCTCCCGCATCATCCACTACACAGAGCCCATCAACTCTCCGAACCTTTCAGACAACACCTGCCACTTTGAGGATGAGAAGATCTGTGGCTATACCCAGGACCTGACAGACAACTTTGACTGGACGCGGCAGAATGCCCTCACCCAGAACCCCAAACGCTCCCCCAACACTGGTCCCCCCACCGACATAAGTGGCACCCCTGAGGGCTACTACATGTTCATCGAGACATCGAGGCCTCGGGAGCTGGGGGACCGTGCAAGGTTAGTGAGTCCCCTCTACAATGCCAGCGCCAAGTTCTACTGTGTCTCCTTCTTCTACCACATGTACGGGAAACACATCGGCTCCCTCAACCTCCTGGTGCGGTCCCGGAACAAAGGGGCTCTGGACACGCACGCCTGGTCTCTCAGTGGCAATAAGGGCAATGTGTGGCAGCAGGCCCATGTGCCCATCAGCCCCAGTGGGCCCTTCCAGATTATTTTTGAGGGGGTTCGAGGCCCGGGCTACCTGGGGGATATTGCCATAGATGACGTCACACTGAAGAAGGGGGAGTGTCCCCGGAAGCAGACGGATCCCAATAAAGTGGTGGTGATGCCGGGCAGTGGAGCCCCCTGCCAGTCCAGCCCACAGCTGTGGGGGCCCATGGCCATCTTCCTCTTGGCGTTGCAGAGAGAATTCGGCNOV46k, CG57409-08SEQ ID NO: 1040451 aaMW at 50381.4kDProtein SequenceMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPEVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRLTPYTTFGAGDMASRIIHYTEPINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPEGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQTDPNKVVVMPGSGAPCQSSPQLWGPMAIFLLALQREFG


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 46B.

TABLE 46BComparison of the NOV46 protein sequences.NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fMEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIREGDTLMLQCNOV46g------------------------------------------------------------NOV46hMEVTCLLLLALIPFHCRGQGVYAPAQAQIVHAGQACVVKEDNISERVYTIREGDTLMLQCNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKNOV46g------------------------------------------------------------NOV46hLVTGHPRPQVRWTKTAGSASDKFQETSVFNETLRIERIARTQGGRYYCKAENGVGVPAIKNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fSIRVDVQYLDEPMLTVHQTVSDVRGNFYQEKTVFLRCTVNSNPPARFIWKRGSDTLSHSQNOV46g------------------------------------------------------------NOV46hSIRVDVQYLDEPMLTVHQTVSDVRGNFYQEKTVFLRCTVNSNPPARFIWKRGSDTLSHSQNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFRLTNTTAPPNOV46g------------------------------------------------------------NOV46hDNGVDIYEPLYTQGETKVLKLKNLRPQDYASYTCQVSVRNVCGIPDKAITFRLTNTTAPPNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLQWSHGPGPLPLGALAQGGTLSIPSVQANOV46g------------------------------------------------------------NOV46hALKLSVNETLVVNPGENVTVQCLLTGGDPLPQLQWSHGPGPLPLGALAQGGTLSIPSVQANOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fRDSGYYNCTATNNVGNPAKKTVNLLVRSMKNATFQITPDVIKESENIQLGQDLKLSCHVDNOV46g------------------------------------------------------------NOV46hRDSGYYNCTATNNVGNPAKKTVNLLVRSMKNATFQITPDVIKESENIQLGQDLKLSCHVDNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fAVPQEKVTYQWFKNGKPARMSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPNOV46g------------------------------------------------------------NOV46hAVPQEKVTYQWFKNGKPARMSKRLLVTRNDPELPAVTSSLELIDLHFSDYGTYLCMASFPNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fGAPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKNOV46g------------------------------------------------------------NOV46hGAPVPDLSVEVNISSETVPPTISVPKGRAVVTVREGSPAELQCEVRGKPRPPVLWSRVDKNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a---------------------------MSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46b------------------------TGTMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46c---------------------------MSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46d----------------------TKLPTMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46e-------------------------TKLSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46fEAALLPSGLPLEETPDGKLRLERVSRDMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46g------------------------------------------------------------NOV46hEAALLPSGLPLEETPDGKLRLERVSRDMSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46i------------------------------------------------------------NOV46j---------------------------MSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46k---------------------------MSGTYRCQTARYNGFNVRPREAQVQLNVQFPPENOV46aVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46bVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46cVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46dVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46eVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46fVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46g------------------------------------------------------------NOV46hVEPSSQDVRQALGRPVLLRCSLLRGSPQRIASAVWRFKGQLLPPPPVVPAAAEAPDHAELNOV46i------------------------------------------------------------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------------------------------------------------------------NOV46hRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLNOV46i------------------------------------------------------------NOV46jRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLNOV46kRLDAVTRDSSGSYECSVSNDVGSAACLFQVSAKAYSPEFYFDTPNPTRSHKLSKNYSYVLNOV46aQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46bQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46cQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46dQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46eQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46fQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46g----------------------LNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46hQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46i----------------------LNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46jQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46kQWTQREPDAVDPVLNYRLSIRQLNQHNAVVKAIPVRRVEKGQLLEYILTDLRVPHSYEVRNOV46aLTPYTTFGAGDMASRIIHYTERQIRWPPVLALRTLSSGPKQGILCRAPHLSSDLVSPLAFNOV46bLTPYTTFGAGDMASRIIHYTEP--------------------------------------NOV46cLTPYTTFGAGDMASRIIHYTEP--------------------------------------NOV46dLTPYTTFGAGDMASRIIHYTEP--------------------------------------NOV46eLTPYTTFGAGDMASRIIHYTEP--------------------------------------NOV46fLTPYTTFGAGDMASRIIHYTERQIRWPPVLALRTLSSGPKQGILCRAPHLSSDLVSPLAFNOV46gLTPYTTFGAGDMASRIIHYTEP---------IN---------------------------NOV46hLTPYTTFGAGDMASRIIHYTEP---------IN---------------------------NOV46iLTPYTTFGAGDMASRIIHYTEP--------------------------------------NOV46jLTPYTTFGAGDMASRIIHYTERQIRWPPVLALRTLSSGPKQGILCRAPHLSSDLVSPLAFNOV46kLTPYTTFGAGDMASRIIHYTEP--------------------------------------NOV46aSAINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46b--INSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46c--INSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46d--INSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46e--INSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46fSAINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46g----SPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46h----SPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46i--INSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46jSAINSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46k--INSPNLSDNTCHFEDEKICGYTQDLTDNFDWTRQNALTQNPKRSPNTGPPTDISGTPENOV46aGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNPLVRSRNKGALNOV46bGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALNOV46cGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALNOV46dGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHNYGKHIGSLNLLVRSRNKGALNOV46eGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALNOV46fGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSPNKGALNOV46gGYYMFIETSRPRELGDRARLVSPLYNACAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALNOV46hGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALNOV46iGYYMFIETSRPRELGDRARLVSPLYNACAKFYCVSFFYHMYGKHIGSLNLLVRSRNKGALNOV46jGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHNYGKHIGSLNPLVRSRNKGALNOV46kGYYMFIETSRPRELGDRARLVSPLYNASAKFYCVSFFYHNYGKHIGSLNLLVRSRNKGALNOV46aDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46bDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46cDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46dDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46eDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPREFNOV46fDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46gDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPR--NOV46hDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46iDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPR--NOV46jDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46kDTHAWSLSGNKGNVWQQAHVPISPSGPFQIIFEGVRGPGYLGDIAIDDVTLKKGECPRKQNOV46aTDPNKVVVMPGSGAPCQSSPQLWG--------------------PMAIFLLALQR-----NOV46bTDPNKVVVMPGSGAPCQSSPQLWG--------------------PMAIFLLALQRLEG--NOV46cTDPNKVVVMPGSGAPCQSSPQLWG--------------------PMAIFLLALQR-----NOV46dTDPNKVVVMPGSGAPCQSSPQLWG--------------------PMAIFLLALQREFG--NOV46eG-----------------------------------------------------------NOV46fTDPNKGARREGAACDGLKFHLSSPMDDGELTDDPIECKHLWIHRVDSKGAQYMLAELNCINOV46g------------------------------------------------------------NOV46hTDPNKGARREGAACDGLKFHLSSPMDDGELTDDPIECKHLWIHRVDSKGAQYMLAELNCINOV46i------------------------------------------------------------NOV46jTDPNKGARREGGGGAESGGSCAWRG-----------------FLSVEGGCSGLNRGSDCLNOV46kTDPNKVVVMPGSGAPCQSSPQLWG--------------------PMAIFLLALQREFG--NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fHVAPRFLVFMDEGHKVGEKDSGGQPFQVYTDFSFYPHAIPEKMSIMPTLQVGKLRLREEENOV46g------------------------------------------------------------NOV46hHVAPRFLVFMDEGHKVGEKDSGGQPFQVYTDFSFYPHAIPEKMSIMPTLQVGKLRLREEENOV46i------------------------------------------------------------NOV46jMMGIRWL-----------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fESAYGHTAAKAPAGTRSWSLNRPPELKSFSLWTNMTQSVREPITGPLQCQTYTHSNTQHLNOV46g------------------------------------------------------------NOV46hESAYGHTAAKARAGTRSWSLNRPPELKSFSLWTNMTQSVREPITGPLQCQTYTHSNTQHLNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fGTWMKLETIILSKLTQEQKTKHHMFSLTTQSAPPSLFSPELPLSSNSRGHASMSLDDDDDNOV46g------------------------------------------------------------NOV46hGTWMKLETIILSKLTQEQKTKHHMFSLTTQSAPPSLFSPELPLSSNSRGHASMSLDDDDDNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fDDDDDDDDDDDDDNDDDDGRKTYRIPPGSDLSACGWVNDHMSQGDSTGPPDEKHSYAKEENOV46g------------------------------------------------------------NOV46hDDDDDDDDDDDDDNDDDDGRKTYRIPPGSDLSACGWVNDHMSQGDSTGPPDEKHSYAKEENOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a------------------------------------------------------------NOV46b------------------------------------------------------------NOV46c------------------------------------------------------------NOV46d------------------------------------------------------------NOV46e------------------------------------------------------------NOV46fTQAKLTGPGISYPGKPSYRLHPSGYTPLEQFREKATGMLTPYVSGSVSSFCFPFPTSESLNOV46g------------------------------------------------------------NOV46hTQAKLTGPGISYPGKPSYRLHPSGYTPLEQFREKATGMLTPYVSGSVSSFCFPFPTSESLNOV46i------------------------------------------------------------NOV46j------------------------------------------------------------NOV46k------------------------------------------------------------NOV46a-----------------------------------NOV46b-----------------------------------NOV46c-----------------------------------NOV46d-----------------------------------NOV46e-----------------------------------NOV46fCSAHANSPSACLCPL--------------------NOV46g-----------------------------------NOV46hCSAHANSPSACLCPLLSGLVSPRLCSLGWHALHLPNOV46i-----------------------------------NOV46j-----------------------------------NOV46k-----------------------------------NOV46a(SEQ ID NO: 1020)NOV46b(SEQ ID NO: 1022)NOV46c(SEQ ID NO: 1024)NOV46d(SEQ ID NO: 1026)NOV46e(SEQ ID NO: 1028)NOV46f(SEQ ID NO: 1030)NOV46g(SEQ ID NO: 1032)NOV46h(SEQ ID NO: 1034)NOV46i(SEQ ID NO: 1036)NOV46j(SEQ ID NO: 1038)NOV46k(SEQ ID NO: 1040)


Further analysis of the NOV46a protein yielded the following properties shown in Table 46C.

TABLE 46CProtein Sequence Properties NOV46aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 11; pos. chg 2; neg. chg 0H-region:length 6; peak value −8.10PSG score:−12.50GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −2.49possible cleavage site: between 61 and 62>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 6.21 (at 260)ALOM score: 6.21 (number of TMSs: 0}MITDISC:discrimination of mitochondrial targeting seqR content:4Hyd Moment(75):3.44Hyd Moment(95):5.36G content:2D/E content:1S/T content:3Score: −1.85Gavel: prediction of cleavage sites for mitochondrial preseqR-2 motif at 28 VRP|RENUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 10.9%NLS Score: −0.47KDEL: ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):47.8%: mitochondrial47.8%: nuclear 4.3%: cytoplasmic>> prediction for CG57409-05 is mit (k = 23)


A search of the NOV46a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 46D.

TABLE 46DGeneseq Results for NOV46aIdentities/NOV46aSimilaritiesGeneseqProtein/Organism/LengthResidues/for theExpectIdentifier[Patent #, Date]Match ResiduesMatched RegionValueABU52337Human GPCR related protein1 . . . 488488/488 (100%)0.0NOV13b - Homo sapiens, 488 aa.1 . . . 488488/488 (100%)[WO200279398-A2, 10-OCT-2002]ABU52338Human GPCR related protein1 . . . 476461/476 (96%)0.0NOV13c - Homo sapiens, 503 aa.1 . . . 476461/476 (96%)[WO200279398-A2, 10-OCT-2002]ABJ10922Human secreted protein (SECP) #18 -1 . . . 458457/458 (99%)0.0Homo sapiens, 869 aa.308 . . . 765 457/458 (99%)[WO200270669-A2, 12-SEP-2002]ABG12100Novel human diagnostic protein1 . . . 458457/458 (99%)0.0#12091 - Homo sapiens, 1219 aa.556 . . . 1013 457/458 (99%)[WO200175067-A2, 11-OCT-2001]ABU52329Human GPCR related protein1 . . . 458456/458 (99%)0.0NOV12a - Homo sapiens, 1386 aa.508 . . . 965 456/458 (99%)[WO200279398-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV46a protein was found to have homology to the proteins shown in the BLASTP data in Table 46E.

TABLE 46EPublic BLASTP Results for NOV46aNOV46aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8NFP4Glycosyl-phosphatidyl-inositol-MAM - 1 . . . 488446/488 (91%)0.0Homo sapiens (Human), 955 aa.508 . . . 955446/488 (91%)Q8HYV1Glycosylphosphatidylinositol anchor 1 1 . . . 488430/488 (88%)0.0protein - Sus scrofa (Pig), 885 aa438 . . . 885435/488 (89%)(fragment).Q8HYV2MAM domain containing 1 . . . 488430/488 (88%)0.0glycosylphosphatidylinositol anchor 1 -439 . . . 886435/488 (89%)Sus scrofa (Pig), 886 aa (fragment).CAB86653DJ402N21.2 (novel protein with MAM176 . . . 488271/313 (86%)e−156domain) - Homo sapiens (Human), 1 . . . 273271/313 (86%)273 aa (fragment).Q8NBE3Hypothetical protein FLJ33358 -276 . . . 476185/201 (92%)e−108Homo sapiens (Human), 242 aa. 12 . . . 212185/201 (92%)


PFam analysis predicts that the NOV46a protein contains the domains shown in the Table 46F.

TABLE 46FDomain Analysis of NOV46aIdentities/PfamNOV46aSimilarities forExpectDomainMatch Regionthe Matched RegionValueig46 . . . 11017/66 (26%)0.009438/66 (58%)MAM286 . . . 451 78/185 (42%) 6.1e−74146/185 (79%) 


Example 47








TABLE 47A








NOV47 Sequence Analysis


















NOV47a, CG57448-01
SEQ ID NO: 1041
2907 bp



DNA Sequence
ORF Start: ATG at 10
ORF Stop: TGA at 2896









GGCCGCTGA
ATGCTAGCTAGAATTCAGCGGCCGCTGAATTCTAGGCGCTGCCGAGGGAATGCGCGCAG



CTCACAGGCCCTGGGAGTGAGCTGGTGCCCGGCGACCTGGCACCCGCGCCTGGATATGGGGCGTCTAC


ATCGTCCCAGGAGCAGCACCAGCTACAGGAACCTGCCGCATCTGTTTCTGTTTTTCCTCTTCGTGGGA


CCCTTCAGCTGCCTCGGGAGTTACAGCCGGGCCACCGAGCTTCTGTACAGCCTAAACGAGGGACTACC


CGCGGGGGTGCTCATCGGCAGCCTGGCCGAGGACCTGCGGCTGCTGCCCAGGTCTGCAGGGAGGCCGG


ACCCGCAGTCGCAGCTGCCAGAGCGCACCGGTGCTGAGTGGAACCCCCCTCTCTCCTTCAGCCTGGCC


TCCCGGGGACTGAGTGGCCAGTACGTGACCCTAGACAACCGCTCTGGGGAGCTGCACACTTCAGCTCA


GGAGATCGACAGGGAGGCCCTGTGTGTTGAAGGGGGTGGAGGGACTGCGTGGAGCGGCAGCGTTTCCA


TCTCCTCCTCTCCTTCTGACTCTTGTCTTTTGCTGCTGGATGTGCTTGTCCTGCCTCAGGAATACTTC


AGGTTTGTGAAGGTGAAGATCGCCATCAGAGACATCAATGACAACGCCCCGCAGTTCCCTGTTTCCCA


GATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATAGAGCATCCTGCTGTGG


ACCCAGATGTAGGCATTAATGGGGTACAGACCTATCGCTTACTGGACTACCATGGTATGTTCACCCTG


GACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGTGCTTTGGACAGGGA


AACCCAGGACCAGTATGTGAGCATCATCATAGCTGAGGATGGTGGGTCTCCACCACTTTTGGGCAGTG


CCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGACTCACAAATCAAT


GTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTGGATAAAGACTT


GGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAGGATTTATTTC


ACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTGGAGTCCCAC


AAGCTCACCATCCTTGCTAATGGACCAGGCTGCATCCCTGCTGTAATCACTGCTCTTGTGTCCATTAT


TAAAGTTATTTTCAGACCCCCTGAAATTGTCCCTCGTTACATAGCAAACGAGATAGATGGTGTTGTTT


ATCTGAAAGAACTGGAACCCGTTAACACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAA


TACAAGGTTAACTGCTACCTGGATGGTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAA


TGAATATTTACTAGAGACCACAAAACCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGG


TGGCTTGGAACTCTGAGGGATTTCATGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGAT


AATGCTCCAATTTTCCTTCAACCCTTAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTT


TTTGACTAAGCTGTATGCTACAGATGCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGAC


CTGATGCTCCATCATATTTTTCCTTAGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGAC


CGAGAAGAGAAAGAAAAGTACAGATACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATC


AGTAGCCACTGTGGCCCTCACAGTGTTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACT


TCAGCTTTTTTGTGCCTGAAAACTTTCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCT


GACGCTGGACGAAATGGATGGGTCGCCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATAC


AGGAAAGGGTATGCTGAGGGCTAAAGTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGG


TTGAAGCTGTTGATGGGGGTGAGCCTGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGAT


ATCAATGACAACCCTCCTCTTGTTTTGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTC


TACTCTGCCAGGCTCCCCGGTTACAGAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCA


TAGCTTACAGCATCATAGGGAGAAGAGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGC


AACATTACTTTGGAAGAGGCATTGCTGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAG


TGATCATGGTTATCCCGAGCCTCTCCACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCA


GTAATGAGAGTTACATTGAGAGTCTTTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCA


CAAATCTCAATAGAACCGACTCATAGGAAGGTAGAATCTGTGTCTTGTATGCCCACCTTAGTAGCTCT


GTCTGTAATAAGCTTGGGTTCCATCACACTGGTCACAGGGATGGGCATATACATCTGTTTAAGGAAAG


GGGAAAAGCATCCCAGGGAAGATGAAAATTTGGAAGTACAGATTCCACTGAAAGGAAAAATTGACTTG


CATATGCGAGAGAGAAAGCCAATGGATATTTCTAATATTTGATATTTCATG














NOV47a, CG57448-01
SEQ ID NO: 1042
962 aa
MW at 106269.4kD



Protein Sequence








MLARIQRPLNSRRCRGNARSSQALGVSWCPATWHPRLDMGRLHRPRSSTSYRNLPHLFLFFLFVGPFS



CLGSYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTGAEWNPPLSFSLASRG


LSGQYVTLDNRSGELHTSAQEIDREALCVEGGGGTAWSGSVSISSSPSDSCLLLLDVLVLPQEYFRFV


KVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVE


ENENGERTPYLIVMGALDRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTV


YGNATVGTPIAAVQAVDKDLGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLT


ILANGPGCIPAVITALVSIIKVIFRPPEIVPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKV


NCYLDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAP


IFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREE


KEKYRYTVPAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAG


RNGWVALSVVNQSDIFVIDTGKGMLRAKVSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDIND


NPPLVLFPQSNMSYLLVLPSTLPGSPVTEVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNIT


LEEALLQTDYGLHRLLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQIS


IEPTHRKVESVSCMPTLVALSVISLGSITLVTGMGIYICLRKGEKHPREDENLEVQIPLKGKIDLHMR


ERKPMDISNI













NOV47b, 247846705
SEQ ID NO: 1043
1377 bp



DNA Sequence
ORF Start: at 1
ORF Stop: end of sequence








GGATCCCCTCGTTACATAGCAAACGAGATAGATGGTGTTGTTTATCTGAAAGAACTGGAACCCGTTAA



CACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATG


GTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAA


CCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCA


TGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCT


TAATAGACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGGTGTATGCTACAGAT


GCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTT


AGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGAT


ACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTG


TTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTT


TCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCG


CCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAA


GTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCC


TGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTT


TGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCCAGGCTCCCCGGTTACA


GAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAG


AGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGC


TGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTC


CACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCT


TTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATA


GGAAGGTAGAACTCGAG














NOV47b, 247846705
SEQ ID NO: 1044
459 aa
MW at 51423.4kD



Protein Sequence








GSPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTK



PMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKVYATD


ADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVPAVDCGKPPRESVATVALTV


LDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSDIFVIDTGKGMLRAK


VSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVT


EVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPL


HSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVELE













NOV47c, 247846708
SEQ ID NO: 1045
1377 bp



DNA Sequence
ORF Start: at 1
ORF Stop: end of sequence








GGATCCCCTCGTTACATAGCAAACGAGATAGATGGTGTGGTTTATCTGAAAGAACTGGAACCCGTTAA



CACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATG


GTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAA


CCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCA


TGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCT


TAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGCTGTATGCTACAGAT


GCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTT


AGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGAT


ACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTG


TTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTT


TCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCG


CCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAA


GTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCC


TGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTT


TGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCTAGGCTCCCCGGTTACA


GAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAG


AGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGC


TGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTC


CACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCT


TTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATA


GGAAGGTAGAACTCGAG














NOV47c, 247846708
SEQ ID NO: 1046
459 aa
MW at 51453.4kD



Protein Sequence








GSPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTK



PMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATD


ADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVPAVDCGKPPRESVATVALTV


LDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSDIFVIDTGKGMLRAK


VSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLLGSPVT


EVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPL


HSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVELE













NOV47d, 237580295
SEQ ID NO: 1047
1377 bp



DNA Sequence
ORF Start: at 1
ORF Stop: end of sequence








GGATCCCCTCGTTACATAGCAAACGAGATAGATGGTGTTGTTTATCTGAAAGAACTGGAACCCGTTAA



CACTCCCATTGCGCTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATG


GTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAA


CCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCA


TGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCT


TAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGCTGTATGCTACAGAT


GCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTT


AGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGAT


ACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTG


TTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTT


TCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCG


CCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAA


GTCTCTTTGGACAGAGAGCAGCAAAGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCC


TGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTT


TGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCCAGGCTCCCCGGTTACA


GAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAG


AGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGC


TGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTC


CACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCT


TTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATA


GGAAGGTAGAACTCGAG














NOV47d, 237580295
SEQ ID NO: 1048
459 aa
MW at 51403.4kD



Protein Sequence








GSPRYIANEIDGVVYLKELEPVNTPIALFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTK



PMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATD


ADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTV


LDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSDIFVIDTGKGMLRAK


VSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVT


EVYAVDKDTGMNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPL


HSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVELE













NOV47e, 237579512
SEQ ID NO: 1049
1377 bp



DNA Sequence
ORF Start: at 1
ORF Stop: end of sequence








GGATCCCCTCGTTACATAGCAAACGAGATAGATGGTGTGGTTTATCTGAAAGAACTGGAACCCGTTAA



CACTCCCATTGCGTTTTTCACCATAAGAGATCCAGAAGGTAAATACAAGGTTAACTGCTACCTGGATG


GTGAAGGGCCGTTTAGGTTATCACCTTACAAACCATACAATAATGAATATTTACTAGAGACCACAAAA


CCTATGGACTATGAGCTACAGCAGTTCTATGAAGTAGCTGTGGTGGCTTGGAACTCTGAGGGATTTCA


TGTCAAAAGGGTCATTAAAGTGCAACTTTTAGATGACAATGATAATGCTCCAATTTTCCTTCAACCCT


TAATAGAACTAACCATCGAAGAGAACAACTCACCCAATGCCTTTTTGACTAAGCTGTATGCTACAGAT


GCCGACAGCGAGGAGAGAGGCCAAGTTTCATATTTTCTGGGACCTGATGCTCCATCATATTTTTCCTT


AGACAGTGTCACAGGAATTCTGACAGTTTCTACTCAGCTGGACCGAGAAGAGAAAGAAAAGTACAGAT


ACACTGTCAGAGCTGTTGACTGTGGGAAGCCACCCAGAGAATCAGTAGCCACTGTGGCCCTCACAGTG


TTGGATAAAAATGACAACAGTCCTCGGTTTATCAACAAGGACTTCAGCTTTTTTGTGCCTGAAAACTT


TCCAGGCTATGGTGAGATTGGAGTAATTAGTGTAACAGATGCTGACGCTGGACGAAATGGATGGGTCG


CCCTCTCTGTGGTGAACCAGAGTGATATTTTTGTCATAGATACAGGAAAGGGTATGCTGAGGGCTAAA


GTCTCTTTGGACAGAGAGCAGCA)AGCTCCTATACTTTGTGGGTTGAAGCTGTTGATGGGGGTGAGCC


TGCCCTCTCCTCTACAGCAAAAATCACAATTCTCCTTCTAGATATCAATGACAACCCTCCTCTTGTTT


TGTTTCCTCAGTCTAATATGTCTTATCTGTTAGTACTGCCTTCTACTCTGCCAGGCTCCCCGGTTACA


GAAGTCTATGCTGTCGACAAAGACACAGGCATGAATGCTGTCATAGCTTACAGCATCATAGGGAGAAG


AGGTCCTAGGCCTGAGTCCTTCAGGATTGACCCTAAAACTGGCAACATTACTTTGGAAGAGGCATTGC


TGCAGACAGATTATGGGCTCCATCGCTTACTGGTGAAAGTGAGTGATCATGGTTATCCCGAGCCTCTC


CACTCCACAGTCATGGTGAACCTATTTGTCAATGACACTGTCAGTAATGAGAGTTACATTGAGAGTCT


TTTAAGAAAAGAACCAGAGATTAATATAGAGGAGAAAGAACCACAAATCTCAATAGAACCGACTCATA


GGAAGGTAGAACTCGAG














NOV47e, 237579512
SEQ ID NO: 1050
459 aa
MW at 51437.4kD



Protein Sequence








GSPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLDGEGPFRLSPYKPYNNEYLLETTK



PMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNDNAPIFLQPLIELTIEENNSPNAFLTKLYATD


ADSEERGQVSYFLGPDAPSYFSLDSVTGILTVSTQLDREEKEKYRYTVEAVDCGKPPRESVATVALTV


LDKNDNSPRFINKDFSFFVPENFPGYGEIGVISVTDADAGRNGWVALSVVNQSDIFVIDTGKGMLRAK


VSLDREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGSPVT


EVYAVDKDTGHNAVIAYSIIGRRGPRPESFRIDPKTGNITLEEALLQTDYGLHRLLVKVSDHGYPEPL


HSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKVELE













NOV47f, CG57448-02
SEQ ID NO: 1051
576 bp



DNA Sequence
ORF Start: at 7
ORF Stop: at 571









GGATCCATCTCGGTGTGGGTCCCGGAAAATGCACCTGTAAACACCCGACTGGCCATAGAGCATCCTGC



TGTGGACCCAGATGTAGGCATTAATGGGGTACAGACCTATCGCTTACTGGACTACCATGGTATGTTCA


CCCTGGACGTGGAGGAGAATGAGAATGGGGAGCGCACCCCCTACCTAATTGTCATGGGTGCTTTGGAC


AGGGAAACCCAGGACCAGTATGTGAGCATCATCATAGCTGAGGATGGTGGGTCTCCACCACTTTTGGG


CAGTGCCACTCTCACCATTGGCATCAGTGACATTAATGACAATTGCCCTCTCTTCACAGACTCACAAA


TCAATGTCACTGTGTATGGGAATGCTACAGTGGGCACCCCAATTGCAGCTGTCCAGGCTGTGGATAAA


GACTTGGGGACCAATGCTCAAATTACTTATTCTTACAGTCAGAAAGTTCCACAAGCATCTAAGGATTT


ATTTCACCTGGATGAAAACACTGGAGTCATTAAACTTTTCAGTAAGATTGGAGGAAGTGTTCTGGAGT


CCCACAAGCTCACCATCCTTGCTAATCTCGAG














NOV47f, CG57448-02
SEQ ID NO: 1052
188 aa
MW at 20271.5kD



Protein Sequence








ISVWVPENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRE



TQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTVYGNATVGTPIAAVQAVDKDL


GTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLTILAN









A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 47B.

TABLE 47BComparison of the NOV47 protein sequences.NOV47aMLARIQRPLNSRRCRGNARSSQALGVSWCPATWHPRLDMGRLHRPRSSTSYRNLPHLFLFNOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aFLFVGPFSCLGSYSRATELLYSLNEGLPAGVLIGSLAEDLRLLPRSAGRPDPQSQLPERTNOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aGAEWNPPLSFSLASRGLSGQYVTLDNRSGELHTSAQEIDREALCVEGGGGTAWSGSVSISNOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aSSPSDSCLLLLDVLVLPQEYFRFVKVKIAIRDINDNAPQFPVSQISVWVPENAPVNTRLANOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDRETQDQYVSINOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQINVTVYGNATVGTPIAAVQAVDKDLNOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLTILANGPGCIPAVNOV47b------------------------------------------------------------NOV47c------------------------------------------------------------NOV47d------------------------------------------------------------NOV47e------------------------------------------------------------NOV47f------------------------------------------------------------NOV47aITALVSIIKVIFRPPEIVPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLNOV47b----------------GSPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLNOV47c----------------GSPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLNOV47d----------------GSPRYIANEIDGVVYLKELEPVNTPIALFTIRDPEGKYKVNCYLNOV47e----------------GSPRYIANEIDGVVYLKELEPVNTPIAFFTIRDPEGKYKVNCYLNOV47f------------------------------------------------------------NOV47aDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNNOV47bDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNNOV47cDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNNOV47dDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNNOV47eDGEGPFRLSPYKPYNNEYLLETTKPMDYELQQFYEVAVVAWNSEGFHVKRVIKVQLLDDNNOV47f------------------------------------------------------------NOV47aDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGNOV47bDNAPIFLQPLIELTIEENNSPNAFLTKVYATDADSEERGQVSYFLGPDAPSYFSLDSVTGNOV47cDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGNOV47dDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGNOV47eDNAPIFLQPLIELTIEENNSPNAFLTKLYATDADSEERGQVSYFLGPDAPSYFSLDSVTGNOV47f------------------------------------------------------------NOV47aILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPNOV47bILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPNOV47cILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPNOV47dILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPNOV47eILTVSTQLDREEKEKYRYTVRAVDCGKPPRESVATVALTVLDKNDNSPRFINKDFSFFVPNOV47f------------------------------------------------------ISVWVPNOV47aENFPGYGEIGVIS-VTDADAGRNGWVALSVVNQSDIFVIDTGKG-------MLRAKVSLDNOV47bENFPGYGEIGVIS-VTDADAGRNGWVALSVVNQSDIFVIDTGKG-------MLRAKVSLDNOV47cENFPGYGEIGVIS-VTDADAGRNGWVALSVVNQSDIFVIDTGKG-------MLRAKVSLDNOV47dENFPGYGEIGVIS-VTDADAGRNGWVALSVVNQSDIFVIDTGKG-------MLRAKVSLDNOV47eENFPGYGEIGVIS-VTDADAGRNGWVALSVVNQSDIFVIDTGKG-------MLRAKVSLDNOV47fENAPVNTRLAIEHPAVDPDVGINGVQTYRLLDYHGMFTLDVEENENGERTPYLIVMGALDNOV47aREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGNOV47bREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGNOV47cREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLLGNOV47dREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGNOV47eREQQSSYTLWVEAVDGGEPALSSTAKITILLLDINDNPPLVLFPQSNMSYLLVLPSTLPGNOV47fRETQDQYVSIIIAEDGGSPPLLGSATLTIGISDINDNCPLFTDSQ--IN-VTVYGNATVGNOV47aSPVTEVYAVDKDTGMNAVIAYSIIGRR-GPRPESFRIDPKTGNITLEEALLQTDYGLHRLNOV47bSPVTEVYAVDKDTGMNAVIAYSIIGRR-GPRPESFRIDPKTGNITLEEALLQTDYGLHRLNOV47cSPVTEVYAVDKDTGMNAVIAYSIIGRR-GPRPESFRIDPKTGNITLEEALLQTDYGLHRLNOV47dSPVTEVYAVDKDTGMNAVIAYSIIGRR-GPRPESFRIDPKTGNITLEEALLQTDYGLHRLNOV47eSPVTEVYAVDKDTGMNAVIAYSIIGRR-GPRPESFRIDPKTGNITLEEALLQTDYGLHRLNOV47fTPIAAVQAVDKDLGTNAQITYSYSQKVPQASKDLFHLDENTGVIKLFSKIGGSVLESHKLNOV47aLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKNOV47bLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKNOV47cLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKNOV47dLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKNOV47eLVKVSDHGYPEPLHSTVMVNLFVNDTVSNESYIESLLRKEPEINIEEKEPQISIEPTHRKNOV47fTILAN-------------------------------------------------------NOV47aVESVSCMPTLVALSVISLGSITLVTGMGIYICLRKGEKHPREDENLEVQIPLKGKIDLHMNOV47bVELE--------------------------------------------------------NOV47cVELE--------------------------------------------------------NOV47dVELE--------------------------------------------------------NOV47eVELE--------------------------------------------------------NOV47f------------------------------------------------------------NOV47aRERKPMDISNINOV47b-----------NOV47c-----------NOV47d-----------NOV47e-----------NOV47f-----------NOV47a(SEQ ID NO: 1042)NOV47b(SEQ ID NO: 1044)NOV47c(SEQ ID NO: 1046)NOV47d(SEQ ID NO: 1048)NOV47e(SEQ ID NO: 1050)NOV47f(SEQ ID NO: 1052)


Further analysis of the NOV47a protein yielded the following properties shown in Table 47C.

TABLE 47CProtein Sequence Properties NOV47aSignalP analysis:Cleavage site between residues 14 and 15PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 7; pos. chg 2; neg. chg 0H-region:length 4; peak value −19.10PSG score:−23.50GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −9.89possible cleavage site: between 29 and 30>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 3INTEGRALLikelihood =Transmembrane  54-70−2.76INTEGRALLikelihood =Transmembrane 416-432−7.38INTEGRALLikelihood =Transmembrane 901-917−3.77PERIPHERALLikelihood = 2.01 (at 188)ALOM score: −7.38 (number of TMSs: 3)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 61Charge difference: −5.0 C(−1.0)-N(4.0)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC: discrimination of mitochondrial targeting seqR content: 7Hyd Moment(75):5.23Hyd Moment(95):11.82G content:2D/E content: 1S/T content:5Score: 2.50Gavel: prediction of cleavage sites for mitochondrial preseqR-2 motif at 85 SRA|TENUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.9%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: LARInoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR:N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: mitochondrial34.8%: endoplasmic reticulum17.4%: nuclear 4.3%: cytoplasmic 4.3%: plasma membrane 4.3%: vesicles of secretory system>> prediction for CG57448-01 is mit (k = 23)


A search of the NOV47a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 47D.

TABLE 47DGeneseq Results for NOV47aNOV47aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABU52605Human NOVX protein, NOV16a -1 . . . 962 962/962 (100%)0.0Homo sapiens, 962 aa.1 . . . 962 962/962 (100%)[WO200281518-A2, 17-OCT-2002]ABP70119Human NOV32a - Homo sapiens, 94716 . . . 962 946/947 (99%)0.0aa. [WO200272771-A2, 19-SEP-2002]1 . . . 947946/947 (99%)AAU09958Human cadherin-1 (CDHN-1) protein -39 . . . 962  924/924 (100%)0.0Homo sapiens, 924 aa.1 . . . 924 924/924 (100%)[WO200179293-A2, 25-OCT-2001]ABP47865Human polypeptide SEQ ID NO 295 -566 . . . 962 396/397 (99%)0.0Homo sapiens, 397 aa.1 . . . 397397/397 (99%)[US2002042386-A1, 11-APR-2002]AAU19645Human novel extracellular matrix566 . . . 962 396/397 (99%)0.0protein, Seq ID No 295 - Homo1 . . . 397397/397 (99%)sapiens, 397 aa. [WO200155368-A1,02-AUG-2001]


In a BLAST search of public sequence databases, the NOV47a protein was found to have homology to the proteins shown in the BLASTP data in Table 47E.

TABLE 47EPublic BLASTP Results for NOV47aNOV47aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8NDN4Hypothetical protein - Homo13 . . . 962949/950 (99%)0.0sapiens (Human), 951 aa. 2 . . . 951949/950 (99%)Q9NRT9Protocadherin 13 - Homo sapiens16 . . . 962946/947 (99%)0.0(Human), 947 aa (fragment). 1 . . . 947946/947 (99%)Q8N6Y1Protocadherin 20 - Homo sapiens39 . . . 962 924/924 (100%)0.0(Human), 924 aa. 1 . . . 924 924/924 (100%)Q8BIV2PROTOCADHERIN 13 homolog -13 . . . 962877/951 (92%)0.0Mus musculus (Mouse), 952 aa. 2 . . . 952913/951 (95%)Q8BIZ0PROTOCADHERIN 13 homolog -39 . . . 962861/925 (93%)0.0Mus musculus (Mouse), 925 aa. 1 . . . 925897/925 (96%)


PFam analysis predicts that the NOV47a protein contains the domains shown in the Table 47F.

TABLE 47FDomain Analysis of NOV47aIdentities/SimilaritiesPfamNOV47afor theExpectDomainMatch RegionMatched RegionValuecadherin225 . . . 32236/111 (32%)1.3e−0873/111 (66%)cadherin336 . . . 42827/110 (25%)0.000466/110 (60%)cadherin551 . . . 64133/107 (31%)6.4e−1969/107 (64%)cadherin655 . . . 74439/107 (36%)5.8e−2069/107 (64%)cadherin762 . . . 85538/112 (34%)  4e−1170/112 (62%)


Example 48

The NOV48 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 48A.

TABLE 48ANOV48 Sequence AnalysisNOV48a, CG57574-03SEQ ID NO: 10531012 bpDNA SequenceORF Start: ATG at 23ORF Stop: TAG at 1010AGAGACCAGGTTCTGAGAAGCAATGGTGACGAAGGCCTTTGTCTTGTTGGCCATCTTTGCAGAAGCCTCTGCAAAATCGTGTGCTCCAAATAAAGCAGATGTCATTCTTGTGTTTTGCTATCCCAAAACCATCATCACCAAAATCCCCGAGTGTCCCTATGGATGGGAAGTTCATCAGCTGGCCCTCGGAGGGCTGTGTTACAATGGGGTCCACGAAGGAGGTTACTACCAATTTGTGATCCCAGATTTATCACCTAAAAACAAGTCCTATTGTGGAACCCAGTCTGAGTACAAGCCACCTATCTATCACTTCTACAGTCACATCGTTTCCAATGACACCACAGTGATTGTAAAAAACCAGCCTGTCAACTACTCCTTCTCCTGCACCTACCACTCCACCTACTTGGTGAACCAGGCTGCCTTTGACCAGAGAGTGGCCACTGTTCACGTGAAGAACGGGAGCATGGGCACATTTGAGAGCCAACTGTCTCTCAACTTCTACACTAATGCCAAGTTCTCCATCAAGAAAGAAGCTCCCTTTGTCCTGGAGGCATCGGAAATCGGTTCAGATCTGTTTGCAGGAGTGGAAGCCAAAGGGTTAAGCATTAGGTTTAAAGTGGTCTTGAACAGCTGTTGGGCCACCCCCTCGGCTGACTTCATGTATCCCTTGCAGTGGCAGCTGATCAACAAGGGCTGCCCCACGGATGAAACCGTCCTCGTGCATGAGAATGGGAGAGATCACAGGGCAACCTTCCAATTCAATGCTTTCCGGTTCCAGAACATCCCCAAACTCTCCAAGGTGTGGTTACACTGTGAGACGTTCATCTGCGACAGTGAGAAACTCTCCTGCCCAGTGACCTGCGATAAACGGAAGCGCCTCCTGCGAGACCAGACCGGGGGAGTCCTGGTCGTGGAGCTCTCCCTGCGGAGCAGGGGATTTTCCAGTCTCTATAGCTTCTCAGATGTTCTCCACCACCTCATCATGATGTTGGGGATTTGTGCCGTGTTATAGNOV48a, CG57574-03SEQ ID NO: 1054329 aaMW at 36955.2kDProtein SequenceMVTKAFVLLAIFAEASAKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEIGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGVLVVELSLRSRGFSSLYSFSDVLHHLIMMLGICAVLNOV48b, CG57574-01SEQ ID NO: 10551026 bpDNA SequenceORF Start: ATG at 85ORF Stop: TAG at 985GATCGAGGCTCAGGCCCTGGAAGGACCGTAAACATTTGGCCAGCTTGGTTTGGATACCTGGCAGAGACCAGGTTCTGAGAAGCAATGGTGACGAAGGCCTTTGTCTTGTTGGCCATCTTTGCAGAAGCCTCTGCAAAATCGTGTGCTCCAAATAAAGCAGATGTCATTCTTGTGTTTTGCTATCCCAAAACCATCATCACCAAAATCCCCGAGTGTCCCTATGGATGGGAAGTTCATCAGCTGGCCCTCGGAGGGCTGTGTTACAATGGGGTCCACGAAGGAGGTTACTACCAATTTGTGATCCCAGATTTATCACCTAAAAACAAGTCCTATTGTGGAACCCAGTCTGAGTACAAGCCACCTATCTATCACTTCTACAGTCACATCGTTTCCAATGACACCACAGTGATTGTAAAAAACCAGCCTGTCAACTACTCCTTCTCCTGCACCTACCACTCCACCTACTTGGTGAACCAGGCTGCCTTTGACCAGAGTGTCAATTTCCTTCCAAAGAATGCCAAGTTCTCCATCAAGAAAGAAGCTCCCTTTGTCCTGGAGGCATCCGAAATCGGTTCAGATCTGTTTGCAGGAGTGGAAGCCAAAGGGTTAAGCATTAGGTTTAAAGTGGTCTTGAACAGCTGTTGGGCCACCCCCTCGGCTGACTTCATGTATCCCTTGCAGTGGCAGCTGATCAACAAGGGCTGCCCCACGGATGAAACCGTCCTCGTGCATGAGAATGGGAGAGATCACAGGGCAACCTTCCAATTCAATGCTTTCCGGTTCCAGAACATCCCCAAACTCTCCAAGGTGTGGTTACACTGTGAGACGTTCATCTGCGACAGTGAGAAACTCTCCTGCCCAGTGACCTGCGATAAACGGAAGCGCCTCCTGCGAGACCAGACCGGGGGAGTCCTGGTCGTGGAGCTCTCCCTGCGGAATGTTCTCCACCACCTCATCATGATGTTGGGGATTTGTGCCGTGTTATAGGAGTTAGCCAGGCAGCTGCCGCTCCTCCACCCACAATAGNOV48b, CG57574-01SEQ ID NO: 1056300 aaMW at 33751.8kDProtein SequenceMVTKAFVLLAIFAEASAKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSCTYHSTYLVNQAAFDQSVNFLPKNAKFSIKKEAPFVLEASEIGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHEATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGVLVVELSLRNVLHHLIMMLGICAVLNOV48c, CG57574-02SEQ ID NO: 10571012 bpDNA SequenceORF Start: ATG at 23ORF Stop: TAG at 1010AGAGACCAGGTTCTGAGAAGCAATGGTGACGAAGGCCTTTGTCTTGTTGGCCATCTTTGCAGAAGCCTCTGCAAAATCGTGTGCTCCAAATAAAGCAGATGTCATTCTTGTGTTTTGCTATCCCAAAACCATCATCACCAAAATCCCCGAGTGTCCCTATGGATGGGAAGTTCATCAGCTGGCCCTCGGAGGGCTGTGTTACAATGGGGTCCACGAAGGAGGTTACTACCAATTTGTGATCCCAGATTTATCACCTAAAAACAAGTCCTATTGTGGAACCCAGTCTGAGTACAAGCCACCTATCTATCACTTCTACAGTCACATCGTTTCCAATGACGCCACAGTGATTGTAAAAAACCAGCCTGTCAACTACTCCTTCTCCTGCACCTACCACTCCACCTACTTGGTGAACCAGGCTGCCTTTGACCAGAGAGTGGCCACTGTTCACGTGAAGAACGGGAGCATGGGCACATTTGAGAGCCAACTGTCTCTCAACTTCTACACTAATGCCAAGTTCTCCATCAAGAAAGAAGCTCCCTTTGTCCTGGAGGCATCCGAAATCGGTTCAGATCTGTTTGCAGGAGTGGAAGCCAAAGGGTTAAGCATTAGGTTTAAAGTGGTCTTGAACAGCTGTTGGGCCACCCCCTCGGCTGACTTCATGTATCCCTTGCAGTGGCAGCTGATCAACAAGGGCTGCCCCACGGATGAAACCGTCCTCGTGCATGAGAATGGGAGAGATCACAGGGCAACCTTCCAATTCAATGCTTTCCGGTTCCAGAACATCCCCAAACTCTCCAAGGTGTGGTTACACTGTGAGACGTTCATCTGCGACAGTGAGAAACTCTCCTGCCCAGTGACCTGCGATAAACGGAAGCGCCTCCTGCGAGACCAGACCGGGGGAGTCCTGGTCGTGGAGCTCTCCCTGCGGAGCAGGGGATTTTCCAGTCTCTATAGCTTCTCAGATGTTCTCCACCACCTCATCATGATGTTGGGGATTTGTGCCGTGTTATAGNOV48c, CG57574-02SEQ ID NO: 1058329 aaMW at 36925.2kDProtein SequenceMVTKAFVLLAIFAEASAKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDATVIVKNQPVNYSFSCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEIGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGVLVVELSLRSRGFSSLYSFSDVLHHLIMMLGICAVLNOV48d, CG57574-04SEQ ID NO: 1059840 bpDNA SequenceORF Start: at 7ORF Stop: at 835GGATCCAAATCGTGTGCTCCAAATAAAGCAGATGTCATTCTTGTGTTTTGCTATCCCAAAACCATCATCACCAAAATCCCCGAGTGTCCCTATGGATGGGAAGTTCATCAGCTGGCCCTCGGAGGGCTGTGTTACAATGGGGTCCACGAAGGAGGTTACTACCAATTTGTGATCCCAGATTTATCACCTAAAAACAAGTCCTATTGTGGAACCCAGTCTGAGTACAAGCCACCTATCTATCACTTCTACAGTCACATCGTTTCCAATGACACCACAGTGATTGTAAAAAACCAGCCTGTCAACTACTCCTTCTCCTGCACCTACCACTCCACCTACTTGGTGAACCAGGCTGCCTTTGACCAGAGAGTGGCCACTGTTCACGTGAAGAACGGGAGCATGGGCACATTTGAGAGCCAACTGTCTCTCAACTTCTACACTAATGCCAAGTTCTCCATCAAGAAAGAAGCTCCCTTTGTCCTGGAGGCATCGGAAATCGGTTCAGATCTGTTTGCAGGAGTGGAAGCCAAAGGGTTAAGCATTAGGTTTAAAGTGGTCTTGAACAGCTGTTGGGCCACCCCCTCGGCTGACTTCATGTATCCCTTGCAGTGGCAGCTGATCAACAAGGGCTGCCCCACGGATGAAACCGTCCTCGTGCATGAGAATGGGAGAGATCACAGGGCAACCTTCCAATTCAATGCTTTCCGGTTCCAGAACATCCCCAAACTCTCCAAGGTGTGGTTACACTGTGAGACGTTCATCTGCGACAGTGAGAAACTCTCCTGCCCAGTGACCTGCGATAAACGGAAGCGCCTCCTGCGAGACCAGACCGGGGGACTCGAGNOV48d, CG57574-04SEQ ID NO: 1060276 aaMW at 31202.3kDProtein SequenceKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEIGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLTNKGCPTDETVLVHENGRDHPATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGNOV48e, CG57574-05SEQ ID NO: 1061821 bpDNA SequenceORF Start: at 1ORF Stop: at 820GGATCCAAATCGTGCGCTCCAAATAAAGCAGATGTCATTCTTGTGTTTTGCTATCCCAAAACCATCATCACCAAAATCCCCGAGTGTCCCTATGGATGGGAAGTTCATCAGCTGGCCCTCGGAGGGCTGTGTTACAATGGGGTCCACGAAGGAGGTTACTACCAATTTGTGATCCCAGATTTATCACCTAAAAACAAGTCCTATTGTGGAACCCAGTCTGAGTACAAGCCACCTATCTATCACTTCTACAGTCACATCGTTTCCAATGACACCACAGTGATTGTAAAAAACCAGCCTGTCAACTACTCCTTCTCCTGCACCTACCACTCCACCTACTTGGTGAACCAGGCTGCCTTTGACCAGAGAGTGGCCACTGTTCACGTGAAGAACGGGAGCATGGGCACATTTGAGAGCCAACTGTCTCTCAACTTCTACACTAATGCCAAGTTCTCCATCAAGAAAGAAGCTCCCTTTGTCCTGGAGGCATCCGAAATCGGTTCAGATCTGTTTGCAGGAGTGGAAGCCAAAGGGTTAAGCATTAGGTTTAAAGTGGTCTTGAACAGCTGTTGGGCCACCCCCTCGGCTGACTTCATGTATCCCTTGCAGTGGCAGCTGATCAACAAGGGCTGCCCCACGGATGAAACCGTCCTCGTGCATGAGAATGGGAGAGATCACAGGGCAACCTTCCAATTCAATGCTTTCCGGTTCCAGAACATCCCCAAACTCTCCAAGGTGTGGTTACACTGTGAGACGTTCATCTGCGACAGTGAGAAACTCTCCTGCCCAGTGACCTGCGATAAACGGAAGCGCCTCCTGCGAGANOV48e, CG57574-05SEQ ID NO: 1062273 aaMW at 30888.0kDProtein SequenceGSKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEIGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLR


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 48B.

TABLE 48BComparison of the NOV48 protein sequences.NOV48aMVTKAFVLLAIFAEASAKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNOV48bMVTKAFVLLAIFAEASAKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNOV48cMVTKAFVLLAIFAEASAKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNOV48d-----------------KSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNOV48e---------------GSKSCAPNKADVILVFCYPKTIITKIPECPYGWEVHQLALGGLCYNOV48aNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSNOV48bNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSNOV48cNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDATVIVKNQPVNYSFSNOV48dNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSNOV48eNGVHEGGYYQFVIPDLSPKNKSYCGTQSEYKPPIYHFYSHIVSNDTTVIVKNQPVNYSFSNOV48aCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEINOV48bCTYHSTYLVNQAAFDQSVNFLPK-----------------N-AKFSIKKEAPFVLEASEINOV48cCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEINOV48dCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEINOV48eCTYHSTYLVNQAAFDQRVATVHVKNGSMGTFESQLSLNFYTNAKFSIKKEAPFVLEASEINOV48aGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHNOV48bGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHNOV48cGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHNOV48dGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHNOV48eGSDLFAGVEAKGLSIRFKVVLNSCWATPSADFMYPLQWQLINKGCPTDETVLVHENGRDHNOV48aRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGVLVVELSNOV48bRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGVLVVELSNOV48cRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGGVLVVELSNOV48dRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLRDQTGG-------NOV48eRATFQFNAFRFQNIPKLSKVWLHCETFICDSEKLSCPVTCDKRKRLLR------------NOV48aLRSRGFSSLYSFSDVLHHLIMMLGICAVLNOV48bLRNVLHHLIMMLGICAVL-----------NOV48cLRSRGFSSLYSFSDVLHHLIMMLGICAVLNOV48d-----------------------------NOV48e-----------------------------NOV48a(SEQ ID NO: 1054)NOV48b(SEQ ID NO: 1056)NOV48c(SEQ ID NO: 105B)NOV48d(SEQ ID NO: 1060)NOV48e(SEQ ID NO: 1062)


Further analysis of the NOV48a protein yielded the following properties shown in Table 48C.

TABLE 48CProtein Sequence Properties NOV48aSignalP analysis:Cleavage site between residues 18 and 19PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 4; pos. chg 1; neg. chg 0H-region:length 9; peak value 11.46PSG score:7.06GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 0.86possible cleavage site: between 17 and 18>>> Seems to have a cleavable signal peptide (1 to 17)ALOM:Klein et al's method for TM region allocationInit position for calculation: 18Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 0PERIPHERALLikelihood = 2.76 (at 25)ALOM score: −1.44 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 8Charge difference: −2.0 C(0.0)-N(2.0)N >= C: N-terminal side will be insideMITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):8.58Hyd Moment(95):5.88G content:0D/E content:2S/T content:3Score: −5.83Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: KRKR (5) at 282pat7: nonebipartite: nonecontent of basic residues: 9.4%NLS Score: −0.16KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: CaaX motif in the C-terminus: CAVLif X is S, A, or M, it will be farnesylatedotherwise, it will be geranylgeranylatedmemYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):33.3%: endoplasmic reticulum22.2%: mitochondrial11.1%: cytoplasmic11.1%: vacuolar11.1%: nuclear11.1%: extracellular, including cell wall>> prediction for CG57574-03 is end (k = 9)


A search of the NOV48a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 48D.

TABLE 48DGeneseq Results for NOV48aNOV48aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE17314Human tectorin beta protein, 1 . . . 329 329/329 (100%)0.0sbg453915TECTORINa - Homo 1 . . . 329 329/329 (100%)sapiens, 329 aa. [WO200198342-A1,27-DEC-2001]ABG72418Cytochrome b45.65 - Unidentified, 41514 . . . 285 84/299 (28%)3e−17aa. [CN1345764-A, 24-APR-2002]27 . . . 318131/299 (43%)AAE17559Human pancreatic tumour protein, GP-77 . . . 282 62/215 (28%)1e−122 - Homo sapiens, 530 aa.270 . . . 472 101/215 (46%)[WO200194409-A2, 13-DEC-2001]AAB54274Human pancreatic cancer antigen77 . . . 282 62/215 (28%)1e−12protein sequence SEQ ID NO: 726 -146 . . . 348 101/215 (46%)Homo sapiens, 406 aa.[WO200055320-A1, 21-SEP-2000]AAR98963Human GP2 pancreatic glycoprotein -77 . . . 282 62/215 (28%)1e−12Homo sapiens, 384 aa. [WO9617873-124 . . . 326 101/215 (46%)A1, 13-JUN-1996]


In a BLAST search of public sequence databases, the NOV48a protein was found to have homology to the proteins shown in the BLASTP data in Table 48E.

TABLE 48EPublic BLASTP Results for NOV48aNOV48aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96PL2Tectorin beta - Homo sapiens1 . . . 329329/329(100%)0.0(Human), 329 aa.1 . . . 329329/329(100%)O08524Beta tectorin - Mus musculus1 . . . 329310/329(94%)0.0(Mouse), 329 aa.1 . . . 329317/329(96%)P54097Beta-tectorin precursor - Gallus1 . . . 329242/329(73%) e−150gallus (Chicken), 329 aa.1 . . . 328286/329(86%)Q95JJ6Hypothetical 45.5 kDa protein -14 . . . 285 85/299(28%)4e−17Macaca fascicularis (Crab eating27 . . . 318 131/299(43%)macaque) (Cynomolgus monkey),415 aa.Q8TCW7Hypothetical protein - Homo sapiens14 . . . 285 84/299(28%)5e−17(Human), 431 aa.43 . . . 334 131/299(43%)


PFam analysis predicts that the NOV48a protein contains the domains shown in the Table 48F.

TABLE 48FDomain Analysis of NOV48aIdentities/SimilaritiesPfamNOV48afor theExpectDomainMatch RegionMatched RegionValuezona_pellucida19 . . . 28355/310(18%)8.2e−49190/310(61%)


Example 49

The NOV49 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 49A.

TABLE 49ANOV49 Sequence AnalysisNOV49a, CG57689-01SEQ ID NO: 10631933 bpDNA SequenceORF Start: ATG at 75ORF Stop: TAA at 1881GGTCGGCTGCCCCGAGGGCTTTGAGCTGGACTCCCAGGGAGCGTTTTGTGTGGGTGAGCGCCCCCAACCCTGGCATGGATGTGGGAGGCCTCTCAGCCTGGGATGGATGGCTTCTCCCAGCCAGCTGGCAGGAGGGCCTGGGAGCGGAGACCACCAGACCCCTTCCTGCCCCAGACAGGGACGAGTGCTCAGGAGGCCCTAGCCCCTGCTCCCATGCCTGCCTTAATGCACCCGGCCGCTTCTCCTGCACCTGCCCCACTGGCTTCGCCCTGGCCTGGGATGACAGGAACTGCAGAGATGTGGACGAGTGTGCGTGGGATGCTCACCTCTGCCGAGAGGGACAGCGCTGTGTGAACCTGCTCGGGTCCTACCGCTGCCTCCCCGACTGTGGGCCTGGCTTCCGGGTGGCTGATGGGGCCGGCTGTGAAGATGTGGACGAATGCCTGGAGGGGTTGGACGACTGTCACTACAACCAGCTCTGCGAGAACACCCCAGGCGGTCACCGCTGCAGCTGCCCCAGGGGTTACCGGATGCAGGGCCCCAGCCTGCCCTGCCTAGATGTCAATGAGTGCCTGCAGCTGCCCAAGGCCTGCGCCTACCAGTGCCACAACCTCCAGGGCAGCTACCGCTGCCTGTGCCCCCCAGGCCAGACCCTCCTTCGCGACGGCAAGGCCTGCACCTCACTGGAGCGGAATGGACAAAATGTGACCACCGTCAGCCACCGAGGCCCTCTATTGCCCTGGCTGCGGCCCTGGGCCTCGATCCCCGGTACCTCCTACCACGCCTGGGTCTCTCTCCGTCCGGGTCCCATGGCCCTGAGCAGTGTGGGCCGGGCCTGGTGCCCTCCTGGTTTCATCAGGCAGAACGGAGTCTGCACAGACCTTGACGAGTGCCGCGTGAGGAACCTGTGTCAGCACGCCTGCCGCAACACTGAGGGCAGCTACCAGTGCCTGTGCCCACGCCGGCTACCGTCTGCTCCCCAGCGGGAAGAACTGCCAGGACATCAACGAGTGCGAGGAGGAGAGCATCGAGTGTGGACCCGGCCAGATGTGCTTCAACACCCGTGGCAGCTACCAGTGTGTGGACACACCCTGTCCTGCCACCTACCGGCAGGGCCCCAGCCCTGGGACGTGCTTCCGGCGCTGCTCGCAGGACTGCGGCACGGGCGGCCCCTCTACGCTGCAGTACCGGCTGCTGCCGCTGCCCCTGGGCGTGCGCGCCCACCACGACGTGGCCCGCCTCACCGCCTTCTCCGAGGTCGGCGTCCCCGCCAACCGCACCGAGCTCAGCATGCTGGAGCCCGACCCCCGCAGCCCCTTCGCGCTGCGTCCGCTGCGCGCGGGCCTTGGCGCGGTCTACACCCGTCGCGCGCTCACCCGCGCCGGCCTCTACCGGCTCACCGTGCGTGCTGCGGCACCGCGCCACCAAAGCGTCTTCGTCTTGCTCATCGCCGTGTCCCCCTACCCCTACTAAACGGGAGAGGGCATTGGCGGCCGCCCTGGCGTGACCCCCGAGGAAGGGGTCGAGGAGAAGCTTGGTCCACGCCACCTGCTGTGGCAAGCGGAGCGTCATCGTCTCCCGCCCCGTGCGTCAGCGAGACCTTGGGTCAACACGACCCTGCGCACAGCCTTGACCCCCGACAGCGAGGACCTGACCTCACAGAGGGAGGCGTCCAGGGCGGCCCTTGGGTGGCCAGTCCCGCAGGCAGGGCCCGGGGAAGCCCGGATCAGACCTCCAGGTCTGATCCGCCCCTCAGTGGGAGCGGGACAGGGACACAGGGCACCTGGACGCGCGGGAGAGGGGGCAGACCCCGCGTTAGGGGTGGCAGCAGCTGTCGCCCGGCCACACCTGGTGGTGTCATTCTGAACCCTGTTGCAATATAAAGGGATTTTTTTTTAACCAACTTGGTTTCTTTGTCTAATTATTTTGCTTTNOV49a, CG57689-01SEQ ID NO: 1064602 aaMW at 65011.8kDProtein SequenceMDVGGLSAWDGWLLPASWQEGLGAETTRPLPAPDRDECSGGPSPCSHACLNAPGRFSCTCPTGFALAWDDRNCRDVDECAWDAHLCREGQRCVNLLGSYRCLPDCGPGFRVADGAGCEDVDECLEGLDDCHYNQLCENTPGGHRCSCPRGYRMQGPSLPCLDVNECLQLPKACAYQCHNLQGSYRCLCPPGQTLLRDGKACTSLERNGQNVTTVSHRGPLLPWLRPWASIPGTSYHAWVSLRPGPMALSSVGRAWCPPGFIRQNGVCTDLDECRVRNLCQHACRNTEGSYQCLCPRRLPSAPQREELPGHQRVRGGEHRVWTRPDVLQHPWQLPVCGHTLSCHLPAGPQPWDVLPALLAGLRHGRPLYAAVPAAAAAPGRARPPRRGPPHRLLRGRRPRQPHRAQHAGARPPQPLRAASAARGPWRGLHPSRAHPRRPLPAHRACCGTAPPKRLRLAHRRVPLPLLNGRGHWRPPWRDPRGRGRGEAWSTPPAVASGASSSPAPCVSETLGQHDPAHSLDPRQRGPDLTEGGVQGGPWVASPAGRARGSPDQTSRSDPPLSGSGTGTQGTWTRGRGGRPRVRGGSSCRPATPGGVILNPVAI


Further analysis of the NOV49a protein yielded the following properties shown in Table 49B.

TABLE 49BProtein Sequence Properties NOV49aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 0; neg. chg 2H-region:length 9; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −3.99possible cleavage site: between 23 and 24>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 7.80 (at 586)ALOM score: 7.80 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):3.89Hyd Moment(95):4.99G content:2D/E content:2S/T content:1Score: −8.14Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: RRPR (4) at 396pat7: PPKRLRL (4) at 450pat7: PKRLRLA (5) at 451bipartite: nonecontent of basic residues: 12.3%NLS Score: 0.55KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):73.9%: nuclear17.4%: cytoplasmic 4.3%: mitochondrial 4.3%: peroxisomal>> prediction for CG57689-01 is nuc (k = 23)


A search of the NOV49a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 49C.

TABLE 49CGeneseq Results for NOV49aNOV49aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABU07377Human protein NOV9 - Homo sapiens,34 . . . 307266/277 (96%)e−1723931 aa. [WO200285922-A2, 31-OCT-3500 . . . 3776 269/277 (97%)2002]ABP69271Human polypeptide SEQ ID NO 1318 -34 . . . 307266/277 (96%)e−172Homo sapiens, 939 aa.508 . . . 784 269/277 (97%)[WO200270539-A2, 12-SEP-2002]ABG34007Human secreted protein #4 encoded by60 . . . 307231/251 (92%)e−146gene 57 - Homo sapiens, 638 aa.233 . . . 483 234/251 (93%)[WO200226931-A2, 04-APR-2002]ABG33919Human secreted protein encoded by60 . . . 307231/251 (92%)e−146gene 57 #1 - Homo sapiens, 575 aa.170 . . . 420 234/251 (93%)[WO200226931-A2, 04-APR-2002]AAE06183Human gene 57 encoded secreted60 . . . 307231/251 (92%)e−146protein fragment, SEQ ID NO: 245 -233 . . . 483 234/251 (93%)Homo sapiens, 638 aa.[WO200151504-A1, 19-JUL-2001]


In a BLAST search of public sequence databases, the NOV49a protein was found to have homology to the proteins shown in the BLASTP data in Table 49D.

TABLE 49DPublic BLASTP Results for NOV49aNOV49aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8BNH3Inferred: hemicentin - Mus34 . . . 546285/541 (52%) e−148musculus (Mouse), 729 aa.229 . . . 729 314/541 (57%)Q8K061Hypothetical protein - Mus34 . . . 307212/278 (76%) e−135musculus (Mouse), 787 aa.355 . . . 632 229/278 (82%)Q96SC3Fibulin-6 - Homo sapiens26 . . . 317124/302 (41%)5e−62(Human), 2673 aa (fragment).2222 . . . 2506 159/302 (52%)Q96RW7Hemicentin - Homo sapiens26 . . . 317122/302 (40%)1e−60(Human), 5636 aa.5185 . . . 5469 158/302 (51%)Q96K89Hypothetical protein FLJ14438 -34 . . . 317114/297 (38%)4e−48Homo sapiens (Human), 741 aa.330 . . . 574 147/297 (49%)


PFam analysis predicts that the NOV49a protein contains the domains shown in the Table 49E.

TABLE 49EDomain Analysis of NOV49aIdentities/SimilaritiesPfamNOV49afor theExpectDomainMatch RegionMatched RegionValueEGF38 . . . 7314/47 (30%)2.9e−0729/47 (62%)EGF123 . . . 16014/47 (30%)0.8624/47 (51%)EGF166 . . . 20115/47 (32%)8.6e−0626/47 (55%)


Example 50

The NOV50 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 50A.

TABLE 50ANOV50 Sequence AnalysisNOV50a, CG58567-01SEQ ID NO: 10653373 bpDNA SequenceORF Start: at 1ORF Stop: TGA at 3190TTGATTGTCCAAGCCACAGATAAAGGGATGCCCAGGCTTTCTAATACGACTGTAATCAAGGTACAGGTGACTGATATAAATGACAATGCCCCAGCTTTTCTCCCCTCTGAAGCAGTGGAAATTACAGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCGATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCCGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAGAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGATACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGACGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATATGAAACCACTGGGATGCCAAGTACCTGCTCACCATTGGTCATGAATGAATGAACAAAATGTTTTCAAGCCGGCAACTCGAGATTGGGCTCATTTTTATCTAAAAGCAAGTGATGTAATTTAGTTAGAGTTTTTAAAACTTCCCCATTAAAGTTTCTCCAATTTCAAAAAAAAAAAAAAAAAAAANOV50a, CG58567-01SEQ ID NO: 10661063 aaMW at 117200.4kDProtein SequenceLIVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTRFLVEASDGGNPDPRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNRTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRYSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQINOV50b, 220087646SEQ ID NO: 10673075 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCTGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAGGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATACGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAAAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGACACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGATGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAGGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATAGTCGACNOV50b, 220087646SEQ ID NO: 10681025 aaMW at 113052.6kDProtein SequenceGSEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTIRSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQIVDNOV50c, 194877960SEQ ID NO: 10693075 bpDNA SequenceORF Start: at 1ORF Stop: end of sequence jGGATCCGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTAATGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGCCCCGTATTACTTCTGGATACAATATCAACAACTCAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCCGACCTGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAAAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAkAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGACACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGATGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATAGTCGACNOV50c, 194877960SEQ ID NO: 10701025 aaMW at 112999.6kDProtein SequenceGSEVMTTSEDSLPGVTVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAXQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRNPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQIVDNOV50d, 192589297SEQ ID NO: 10711899 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCTGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAAAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTGTCGACNOV50d, 192589297SEQ ID NO: 1072633 aaMW at 69903.4kDProtein SequenceGSEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSVDNOV50e, CG58567-02SEQ ID NO: 10732956 bpDNA SequenceORF Start: ATG at 57ORF Stop: TGA at 2934CTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCTGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTCCCTTTCAGTAAATAAAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGACACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGATGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATATGAAACCACTGGGATGCCAAGTANOV50e, CG58567-02SEQ ID NO: 1074959 aaMW at 105826.6kDProtein SequenceMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPSLSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQINOV50f, CG58567-03SEQ ID NO: 10753075 bpDNA SequenceORF Start: at 7ORF Stop: at 3070GGATCCGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGCCCCGTATTACTTCTGGATACAATATCAACAACTCAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCCGACCTGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAAAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGACACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGATGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATAGTCGACNOV50f, CG58567-03SEQ ID NO: 10761021 aaMW at 112641.2kDProtein SequenceEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQINOV50g, CG58567-04SEQ ID NO: 10771899 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCTGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAAAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTGTCGACNOV50g, CG58567-04SEQ ID NO: 1078633 aaMW at 69903.4kDProtein SequenceGSEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLPALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSVDNOV50h, CG58567-05SEQ ID NO: 10799193 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 9010ATGGAGAAATGTGGGCTTAAAGAGGAAGGCAGTTACGCGGACATAGATCCAGTTGCCCACGATCCGGACGCCGGACTGTTCAGCACTCAGGGCTACACCCTGGTGCAACCGTCCGACCTGCCCAAGGACCCCGCAGGCCCGTTCTTCCAGTTGCGCTACCGGACTCCGGGGCCACTACCGTCACCGCTTTTGCCAGGCTCCTCGTCACCCCTGGAGCCTCTAGATCTGGTGCTGCTGCGGCGCTTGGACCGAGAGGAGGCGGCGGCGCACCGGCTGCAGATCGAGGCATGGGACGGCGGCCGACCCCGGCGCACCGGCCTCCTGAGCGTGGAGCTGCGCGTGCTGGATGAGAACGACAACCCGCCGGTCTTTGAGCAGGACGAGTACCGCGCCGCGGTGCGCGAGGACGCCCAGCCGGGCGCCGAGGTCTGTCGCGTGCGCGCCACCGACCGCGACCTGGGGCCCAATGGCTTCGTGCGCTACAGCGTCCGCGCCCGGCAAGTGCCTGGGGCGGGTAGCGGCGGCGGGGCACTGGGCGACGCGGCCTACTTCGCGGTGGAGGAGCTGAGCGGCGTGGTGCGAGTGTGGAGACCTCTGGACCGCGAGGCACAGGCCTGGCACCAGTTGGTGGTGGAGGCCCGCGATGGAGGCGCCGAGCCTGAGGTTGCCACGGTGCGCGTGTCCATCGCCGTGCTGGACGTGAATGACAACCGGCCAGCAATTCACGTGCTCTTTCTCACAGAGGGAGGCGTCGCCCGTGTCTCTGAAGGCGCCCGACCGGGCGACTACGTGGCTCGCGTCTCGGTGTCTGACGCGGACGGGGTAATAGGCAAAATTACAGCTATTGACATGGACTCTGGAAAGAATGGACAGCTATTATATTTCCTTTTGTCTGATGGAAAATTCTTCAAGATGAATCCTAATACAGGAGAGTTAATCAATTGGGTGGCACTGGATCGTGAGCACCGGGGGCACCATGAGATGACTGTGCTAGTGACAGACCGCGGCTCCCCACCACGAAACGCCACCATGGCGGTTTACGTCTCAGTTACTGACATCAATGATAACAGGCCCTTCTTCCCCCAGTGTCTCCCTGGAAAGGAGTTACACGTGAAGGTTCTGGAAGGTCAACCAGTAAATATGTTGGTTACAACTGTGTTTGCAAAGGATCCTGATGAAGGAAATAATGCAGAAGTTACATACTCAGTATCTTCAGAAGATAGTTCTGATCACTTTAAGATTGACGCCAACAATGGTGAAATAAGAACAACCACAATACTTTCGTATGATTATAGACCTTCCTACAGAATGAGTGTCATTGCCACTGACCAGGGAGTGCCTCCTCTTCAAGGACAGGCAGTTGTTAATATTCAGGAACTTGATTATGAGACGACATCTCATTATCTTTTCAGAGTGATTACTACAGACCATAGCAAAAACCTTTCCCTGAGTAGCACAGTCTTCCTTAGTATCGATGTGGAAGATCAGAATGACCATTCCCCATCTTTCCAGGATGAGCTCATTGTGATCAGTGTAGAGGAGAATGTTCCCATAGGAACCCTGGTGTATGTCTTCAATGCCAAAGATGATGACGGCAGTTTTTTGAACAGTAGAATACAATACTACATTGAATCCCACAACCCTGGCACGAATCCATTTCTCATCCACCCCTCATTTGGCACACTAGTCACTGTGTCCCGTCTTGACAGAGAAAGCATTCCAACTGTCATCCTGACAGTAACAGCATCTGATCAGGCTGTGAATGTGACAGACCGGCGACTGAGATCACTGACAGCACAAATAGTGATTTTGGATGTAAATGACCACAACCCCACTTTTATTTCTTTCCCCAATGCCCATGTCAAAGAGGATGTCACAGTGGGCTCCTTGGTCCACCACATAACTGCTCACGATCCAGACGAAGGAAGGAATGGAAAAGTAACATACAGCATCCTCTCAGGAAATGAAAACATGACGTTTATGCTAGATGAGTCATCAGGCTTACTAACCACAACCTGTCCTTTGGATTATGAAATGAAAACTCAGCATATTCTGACTGTTCTGGCACTGGATGATGGCACACCAGCACTTTCTTCATCCCAGACTTTGACAGTTACTGTTCTTGATGTAAATGATGAAGCTCCAGTATTTAAGCAGCACCTGTATGAAGCCTCAGTGAAAGAAAACCAAAATCCAGGGGAGTTTGTTACCAGGGTTGAAGCTCTGGACAGAGATTCAGTGTTCCTTAATACTAGAGAGCTTAACATGTGTTTTCTAGCATTCTACGATGCAGTTTTTAAAAATGGTGGGCTAAGTGCCCAAGCCTTTGTTCGTGTGGACCTGGAGGACGTGAATGATAATCATCCTGTGTTTAACCCATCAACCTATGTGACGAGCATCAGTGATGAGACCCAGCCAGGCACCGAGATCATCAATGTTCTTGCCACTGACCAGGACTCTGGGATATATGGGACAGTGGCTTATGAGCTTATTCCAGGAAACGTGTCGTCCCTTTTTACCATTGACTCCACCACAGGAATTATTTACTTAACATTACCTCTTAGTCATTTGGAATCTACCACACTTTCGTTGATGGTCTCTGCTCAAGACGGTGGTGGGCTCACAGCTGTCATTAATGCCGATGTCACCATACACATTTTCCAGACAACTCTGGCACCTGCTGAGTTTGAAAGGCCTAAGTACACTTTCTTAGTTTATGAAGATGTGCCTGAAGATAGTCCCATTGGAACAGTGAAAGCAAGAGAGCCCTTGAATCCACCTAGGAGCTCTGTAATACACCTGCAAGTTAGAGTTTTGGATGCCAATGACCACAGTCCTTCTTTTCCCACACTTTATTACCAGTCCTCTGTGAGAGAAGATGCTGAAGTGGGAACAGTGGTTCTTGTGCTTTCAGCTGTGGACAAGGATGAAGGCCTGAATGGGCAAACTGAGTATTTTCTGACTGATGAGGCTTGTGGTGCATTCACCATTGATCCTATGTCAGGCACATTGAAAACCAGCAACACCCTCGACCGTGAAGCCAGATCTCAGCATACATTTAGTGCTGTGGCCAGAGACTGTAGCATCCAGGGTTCACGAAGCACCACTGTAATTATAAAAGTATATGTCACTGATGTTAATGACAATGATCCAGTTTTGGAACAGAACCCTTTTGATGTGTTTCTTTCCCCCGAGTCGCCTACAAACCAGACAACTGTCATTGTGAGAGCTGATGACCTGGACTTGGGGCCCAATGGAACTGTGGACTCCGATGACTCCCCGCTGCTGGACGACTTCCACGTGCACCCGGACACCGGCATCATCCGCACTGCGCGGCGCCTGGACCGCGAGCGGCGGGACCACTACAGCTTCGTCGCCGCCACGCTGCTGGGCGCTGTGGTGCAGGTGGAGATTCGCGTCAACGACGTGAATGACCACTCGCCCCGCTTTCCCCTCGACTCCCTGCAACTCGACGTCTCCGAGCTCAGCCCGCCAGGGACCGCCTTCCGCCTGCCAGTTGCCCACGATCCGGACGCCGGACTGTTCAGCACTCAGGGCTACACCCTGGTGCAACCGTCCGACCTGCCCAAGGACCCCGCAGGCCCGTTCTTCCAGTTGCGCTACCGGACTCCGGGGCCACTACCGTCACCGCTTTTGCCAGGCTCCTCGTCACCCCTGGAGCCTCTAGATCTGGTGCTGCTGCGGCGCTTGGACCGAGAGGAGGCGGCGGCGCACCGGCTGCAGATCGAGGCATGGGACGGCGGCCGACCCCGGCGCACCGGCCTCCTGAGCGTGGAGCTGCGCGTGCTGGATGAGAACGACAACCCGCCGGTCTTTGAGCAGGACGAGTCCCGCGCCGCGGTGCGAGAGCACGCCCAGCCGGGCGCCGAGGTCTGTCGCGTGCGCGCCACCGCCCGCGACGTGGGGCCCAATGGCTTCGTGCGCTACAGCGTCCGCGCCCGGCAAGTGCCTGGGGCGGGTAGCGGCGGCGGGGCACTGGGCGACGCGGCCTACTTCGCGTTGGTGGTGGAGGCCCGCGATGGAGGCGCCGAGCATGAGGTTGCCGCGGTGCGTGTGTCCATCGCCGTGCTGGACGTGAATGACAACCGGCCAGCAATTCACGTGCTCTTTCTCACAGAGGGAGGTGTCGCCCGTGTCTCTGAAGGCGCCCGCCCGGGCGACTACGTGGCTCGCGTCTCGGTGTCTGACGCGGGCGGTGACTGGGAGAAGGAAGATGAGGCCACAGGGGAGCTTGGTGTGACAGCCTCTGATGCAGATTCAGGACTCTATGGCTTTATTGAATATTCTCTTTATGATGGATTCCTGAGCTATGAAGCACCTCAGGCATTCCGGATCGACCCTCATGATGGGCAAATCTGTGTTTCTCAAGATATCGACAGGGAAAGGGATCCAGCTACCTATGATCTCCTGGTGGAAGCTAAGGATGGGTTTGAAATCATGCCAGGTGCTTCATTTGAATTATTCGAGATAAATTCTGACACTGGAGAGGTAGTGACAACCACCATACTTGACAGAGAAATTCAAGAAGTCTTCACCCTTCGAGTACTAGTACGAGATGGGGGATTCCCTTCATTGTCCAGCACCACAACAATCCTCTGCACTGTTGAAGATGAAAACGATCACGCACCAGAGTTTATTGTTTCCAGTTATGACATTGAGGTTCTGGAAAACCAGGAACCAGAGGTTGTCTATACGGTTTTAGCCTCTGATATGGATGCTGGCAATAACAGAGCTGTTGAATATCACATAATTGACTCCTCAGAACCAATCTTTTACAGGATTTCTTCTGGTGATCTCGGCGGAAAGTTCTCCATTCACCCGCGGCTGGGCACTATTCGCACCCGGAAGCCCCTGGATCACGAGACGCAGCCCGTGGTTGTGCTCACGGTGCAGGCGCAGCTCGGCAGCGCCCCAGCCTGCAGCAGCACCGAGGTCAACATAACAGTCATGGATGTCAATGACAACCACCCAGCGTTCCTCAGGACCTCGGATGAGATTAGAATATCCCAGACCACGCCCCCTGGCACAGCCTTGTACCTCGCACGTGCGGAAGACAGAGACAGTGGGCGGAACGGACTCATCCGGTACTCCATCGCCAGCCCGCAGCCAGGCGTCTTTGCCATCGACAGAGCCCTGGGGGTGCTGTTCCTCAACGGCAGCCTGGGCGCGGGCGAGCAGCGGGAGCTCACGCTGACTCTCAGGGCCGAGGACCAAGGCGTGCATCCTCAGGCAGCCCTGCTGGTGCTGACAGTCGTTATCGAGAAACGCGAACACAGCCCATCCTGGACTTTCGAACATTTGGTCTATCAAGTGGAAGTCAGTACTTCTATTGTGACTGTTAAAGCTTTTGCTCCTGACTCAATTCAGGACAGCATGAAATATTCAATTTTTAGTGGAAATGAAGATGGAGTTCTTTCCCTGTGCTCTAAGTCAGGTGTGGTGAACTGCCTTGCTTCTCTCAGTCACACAGACTTTCTCTCCCTGAAATTTGAATCTTCGGTGAAGGGACACCAAGACAGAGACAAATTACAGCCAATTCATCTTGATGACAACAACTCAAAGAAGCTGTGCTTTACATTCCCTAGAGCCACTCAGGCTCTTGTATTCACTGGGCACTGTCTTTCTGATACATCTCTCCCCGGTTGGGTTTTTGCTACCGACTTGGACAGTGGTTTGAACGGCCTGATTGAGTATTCTATTCTGTCTGGCAACCAAGAAGAAGCATTCCAGATTGATGCACTGAGTGGTGTGATAACAACAAAAGCGATTCTAGATTACGAGCTCACCAGCTCCTACAGCTTGATTGTCCAAGCCACAGATAAAGGGATGCCCAGGCTTTCTAATACGACTGTAATCAAGGTACAGGTGACTGATATAAATGACAATGCCCCAGCTTTTCTCCCCTCTGAAGCAGTGGAAATTACAGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCGATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCCGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAGAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGATACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGACGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATATGAAACCACTGGGATGCCAAGTACCTGCTCACCATTGGTCATGAATGAATGAACAAAATGTTTTCAAGCCGGCAACTCGAGATTGGGCTCATTTTTATCTAAAAGCAAGTGATGTAATTTAGTTAGAGTTTTTAAAACTTCCCCATTAAAGTTTCTCCAATTTCAAAAAAAAAAAAAAAAAANOV50h, CG58567-05SEQ ID NO: 10803003 aaMW at 329184.0kDProtein SequenceMEKCGLKEEGSYADIDPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPFFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLLSVELRVLDENDNPPVFEQDEYRAAVREDAQPGAEVCRVRATDRDLGPNGFVRYSVRARQVPGAGSGGGALGDAAYFAVEELSGVVRVWRPLDREAQAWHQLVVEARDGGAEPEVATVRVSIAVLDVNDNRPAIHVLFLTEGGVARVSEGARPGDYVARVSVSDADGVIGKITAIDMDSGKNGQLLYFLLSDGKFFKMNPNTGELINWVALDREHRGHHEMTVLVTDRGSPPRNATMAVYVSVTDINDNRPFFPQCLPGKELHVKVLEGQPVNMLVTTVFAKDPDEGNNAEVTYSVSSEDSSDHFKIDANNGEIRTTTILSYDYRPSYRMSVIATDQGVPPLQGQAVVNIQELDYETTSHYLFRVITTDHSKNLSLSSTVFLSIDVEDQNDHSPSFQDELIVISVEENVPIGTLVYVFNAKDDDGSFLNSRIQYYIESHNPGTNPFLIHPSFGTLVTVSRLDRESIPTVILTVTASDQAVNVTDRRLRSLTAQIVILDVNDHNPTFISFPNAHVKEDVTVGSLVHHITAHDPDEGRNGKVTYSILSGNENMTFMLDESSGLLTTTCPLDYEMKTQHILTVLALDDGTPALSSSQTLTVTVLDVNDEAPVFKQHLYEASVKENQNPGEFVTRVEALDRDSVFLNTRELNMCFLAFYDAVFKNGGLSAQAFVRVDLEDVNDNHPVFNPSTYVTSISDETQPGTEIINVLATDQDSGIYGTVAYELIPGNVSSLFTIDSTTGIIYLTLPLSHLESTTLSLMVSAQDGGGLTAVINADVTIHIFQTTLAPAEFERPKYTFLVYEDVPEDSPIGTVKAREPLNPPRSSVIHLQVRVLDANDHSPSFPTLYYQSSVREDAEVGTVVLVLSAVDKDEGLNGQTEYFLTDEACGAFTIDPMSGTLKTSNTLDREARSQHTFSAVARDCSIQGSRSTTVIIKVYVTDVNDNDPVLEQNPFDVFLSPESPTNQTTVIVRADDLDLGPNGTVDSDDSPLLDDFHVHPDTGIIRTARRLDRERRDHYSFVAATLLGAVVQVEIRVNDVNDHSPRFPLDSLQLDVSELSPPGTAFRLPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPFFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLLSVELRVLDENDNPPVFEQDESRAAVREHAQPGAEVCRVRATARDVGPNGFVRYSVRARQVPGAGSGGGALGDAAYFALVVEARDGGAEHEVAAVRVSIAVLDVNDNRPAIHVLFLTEGGVARVSEGARPGDYVARVSVSDAGGDWEKEDEATGELGVTASDADSGLYGFIEYSLYDGFLSYEAPQAFRIDPHDGQICVSQDIDRERDPATYDLLVEAKDGFEIMPGASFELFEINSDTGEVVTTTILDREIQEVFTLRVLVRDGGFPSLSSTTTILCTVEDENDHAPEFIVSSYDIEVLENQEPEVVYTVLASDMDAGNNRAVEYHIIDSSEPIFYRISSGDLGGKFSIHPRLGTIRTRKPLDHETQPVVVLTVQAQLGSAPACSSTEVNITVMDVNDNHPAFLRTSDEIRISQTTPPGTALYLARAEDRDSGRNGLIRYSIASPQPGVFAIDPALGVLFLNGSLGAGEQRELTLTLRAEDQGVHPQAALLVLTVVIEKREHSPSWTFEHLVYQVEVSTSIVTVKAFAPDSIQDSMKYSIFSGNEDGVLSLCSKSGVVNCLASLSHTDFLSLKFESSVKGHQDRDKLQPIHLDDNNSKKLCFTFPRATQALVFTGHCLSDTSLPGWVFATDLDSGLNGLIEYSILSGNQEEAFQIDALSGVITTKAILDYELTSSYSLIVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTRFLVEASDGGNPDPRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNRTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRYSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQINOV50i, CG58567-06SEQ ID NO: 108110267 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 10084ATGGAGAAATGTGGGCTTAAAGAGGAAGGCAGTTACGCGGACATAGATCCAGTTGCCCACGATCCGGACGCCGGACTGTTCAGCACTCAGGGCTACACCCTGGTGCAACCGTCCGACCTGCCCAAGGACCCCGCAGGCCCGTTCTTCCAGTTGCGCTACCGGACTCCGGGGCCACTACCGTCACCGCTTTTGCCAGGCTCCTCGTCACCCCTGGAGCCTCTAGATCTGGTGCTGCTGCGGCGCTTGGACCGAGAGGAGGCGGCGGCGCACCGGCTGCAGATCGAGGCATGGGACGGCGGCCGACCCCGGCGCACCGGCCTCCTGAGCGTGGAGCTGCGCGTGCTGGATGAGAACGACAACCCGCCGGTCTTTGAGCAGGACGAGTACCGCGCCGCGGTGCGCGAGGACGCCCAGCCGGGCGCCGAGGTCTGTCGCGTGCGCGCCACCGACCGCGACCTGGGGCCCAATGGCTTCGTGCGCTACAGCGTCCGCGCCCGGCAAGTGCCTGGGGCGGGTAGCGGCGGCGGGGCACTGGGCGACGCGGCCTACTTCGCGGTGGAGGAGCTGAGCGGCGTGGTGCGAGTGTGGAGACCTCTGGACCGCGAGGCACAGGCCTGGCACCAGTTGGTGGTGGAGGCCCGCGATGGAGGCGCCGAGCCTGAGGTTGCCACGGTGCGCGTGTCCATCGCCGTGCTGGACGTGAATGACAACCGGCCAGCAATTCACGTGCTCTTTCTCACAGAGGGAGGCGTCGCCCGTGTCTCTGAAGGCGCCCGACCGGGCGACTACGTGGCTCGCGTCTCGGTGTCTGACGCGGACGGTGACTGGGAGAAGGAAGATGAGGCCACAGGGGAGCTTGGTGTGGGTCTTGGAGACGGGAGCATCTCTCTGTCCTTGGAAGGCGGAGAGGGAGACTTCGCGTTGCTACCCGGCGGCCCCCCAGGGGTATTTTTCCTTTGCGTGGAGGGGCCCCTGGACAGAGAGAGCCGCGATCTGTATGAGTTACTACTGGTGGCCACGGACGCGGGGTCCCCGCCGCTGAGCACGGAGGAGACGCTGCTACTCCGGGTCGCTGACCTCAATGACCAACCACCTCTCTTCAGCCAACAGCATTACAAGGCCTCAGTGTCCGAGGCCGCGGCCCCTGGCACTGTAGTCATGTGGGTCAGCGCCTCCGATGCCGACGAGGCAGGCAGTGATCACGCCTGGCTGCGCTACACTGTAGTCCAACTCTCGGCTCCCTGCAATCTCGGCTCCCTGCAATCAAAGATGGTCCACACCGCAGAGTGTGGACCATCTTTTGCCATTGATTCCGAAAGCGGTGCGATCAGCACTATCCGGACTCTAGACCGAGAGGTCCAGGAGGCGGTGGAGCTGAAAGTGGTGGCCCAGGACCTCGGAGAGCCCCCACTCTCTGCCACCTGCCTGGTGAGCATCACCGTAGATGATGTGAATGACAATGAGCCCATCTTCTGGAGGCAGGTGTACAATGCCACCATTGCAGAGCATGCCCCGGTTGGACACTGCTTTCTGCAGCTTATATCTGCTCAAGTTGCCTCTGTCAAAATCAAACACAAACACAAGGAGATACACGAGAAACACAATCTTGCCTATATTTCCTGTCCAGCAGGCACCATCTATGTCATAACCTGGGCAGATGGTGCTGCTGCCTTTAGTGGGACAGACTTTGCATTCAGTTCTGATGAACTTCAAGCCTTTGTTCTCAAGTCTCTGTTCTGTGAATTAGGAGAAGGAGAGTTAATCAATTGGGTGGCACTGGATCGTGAGCACCGGGGGCACCATGAGATGACTGTGCTAGTGACAGACCGCGGCTCCCCACCACGAAACGCCACCATGGCGGTTTACGTCTCAGTTACTGACATCAATGATAACAGGCCCTTCTTCCCCCAGTGTCTCCCTGGAAAGGAGTTACACGTGAAGGTTCTGGAAGGTCAACCAGTAAATATGTTGGTTACAACTGTGTTTGCAAAGGATCCTGATGAAGGAAATAATGCAGAAGTTACATACTCAGTATCTTCAGAAGATAGTTCTGATCACTTTAAGATTGACGCCAACAATGGTGAAATAAGAACAACCACAATACTTTCGTATGATTATAGACCTTCCTACAGAATGAGTGTCATTGCCACTGACCAGGGAGTGCCTCCTCTTCAAGGACAGGCAGTTGTTAATATTCAGGTGATCCCACTATCCAAAGGGAGAGCAATCATGTCTCAGAATATTAGACATTTAATTATACCAGAAAATTTGAAGCCCACAAAAATAATGAGCTTGATAAAGTCATCTGATCACCTTCAACAACATTATAATGGAAAGTTACATTTTAGTATTGTTGCAGATGATAAGGATGGACACTTTGAAATAGACAGCTCAACCGGAGACTTGTTTCTTTCTAAGGAACTTGATTATGAGACGACATCTCATTATCTTTTCAGAGTGATTACTACAGACCATAGCAAAAACCTTTCCCTGAGTAGCACAGTCTTCCTTAGTATCGATGTGGAAGATCAGAATGACCATTCCCCATCTTTCCAGGATGAGCTCATTGTGATCAGTGTAGAGGAGAATGTTCCCATAGGAACCCTGGTGTATGTCTTCAATGCCAAAGATGATGACGGCAGTTTTTTGAACAGTAGAATACAATACTACATTGAATCCCACAACCCTGGCACGAATCCATTTCTCATCCACCCCTCATTTGGCACACTAGTCACTGTGTCCCGTCTTGACAGAGAAAGCATTCCAACTGTCATCCTGACAGTAACAGGATGTAAATGACCACAACCCCACTTTTATTTCTTTCCCCAATGCCCATGTCAAAGAGGATGTCACAGTGGGCTCCTTGGTCCACCACATAACTGCTCACGATCCAGACGAAGGAAGGAATGGAAAAGTAACATACAGCATCCTCTCAGGAAATGAAAACATGACGTTTATGCTAGATGAGTCATCAGGCTTACTAACCACAACCTGTCCTTTGGATTATGAAATGAAAACTCAGCATATTCTGACTGTTCTGGCACTGGATGATGGCACACCAGCACTTTCTTCATCCCAGACTTTGACAGTTACTGTTCTTGATGTAAATGATGAAGCTCCAGTATTTAAGCAGCACCTGTATGAAGCCTCAGTGAAAGAAAACCAAAATCCAGGGGAGTTTGTTACCAGGGTTGAAGCTCTGGACAGAGATTCAGGTATGAGGCTGAATGGAGATCCAGACAGGGAGCTGTGTGCAGGAGGGAACCCTCTTGGAAGCAGGGCCCCTCCTGGAAGCAGGACCCCTCCTGAAGGTGGGCTAAGTGCCCAAGCCTTTGTTCGTGTGGACCTGGAGGACGTGAATGATAATCATCCTGTGTTTAACCCATCAACCTATGTGACGAGCATCAGTGATGAGACCCAGCCAGGCACCGAGATCATCAATGTTCTTGCCACTGACCAGGACTCTGGGATATATGGGACAGTGGCTTATGAGCTTATTCCAGGAAACGTGTCGTCCCTTTTTACCATTGACTCCACCACAGGAATTATTTACTTAACATTACCTCTTAGTCATTTGGAATCTACCACACTTTCGTTGATGGTCTCTGCTCAAGACGGTGGTGGGCTCACAGCTGTCATTAATGCCGATGTCACCATACACATTTTCCAGACAACTCTGGCACCTGCTGAGTTTGAAAGGCCTAAGTACACTTTCTTAGTTTATGAAGATGTGCCTGAAGATAGTCCCATTGGAACAGTGAAAGCAAGAGAGCCCTTGAATCCACCTAGGAGCTCTGTAATACACCTGCAAGTTAGAGTTTTGGATGCCAATGACCACAGTCCTTCTTTTCCCACACTTTATTACCAGTCCTCTGTGAGAGAAGATGCTGAAGTGGGAACAGTGGTTCTTGTGCTTTCAGCTGTGGACAAGGATGAAGGCCTGAATGGGCAAACTGAGTATTTTCTGACTGATGAGGCTTGTGGTGCATTCACCATTGATCCTATGTCAGGCACATTAGAAACCAGCAACACCCTCGACCGTGAAGCCAGATCTCAGCATACATTTAGTGCTGTGGCCAGAGACTGTAGCATCCAGGGTTCACGAAGCACCACTGTAATTATAAAAGTATATGTCACTGATGTTAATGACAATGATCCAGTTTTGGAACAGAACCCTTTTGATGTGTTTCTTTCCCCCGAGTCGCCTACAAACCAGACAACTGTCATTGTGAGAGCTGATGACCTGGACTTGGGGCCCAATGGAACTGTGGACTCCGATGACTCCCCGCTGCTGGACGACTTCCACGTGCACCCGGACACCGGCATCATCCGCACTGCGCGGCGCCTGGACCGCGAGCGGCGGGACCACTACAGCTTCGTCGCCGCCACGCTGCTGGGCGCTGTGGTGCAGGTGGAGATTCGCGTCAACGACGTGAATGACCACTCGCCCCGCTTTCCCCTCGACTCCCTGCAACTCGACGTCTCCGAGCTCAGCCCGCCAGGGACCGCCTTCCGCCTGCCAGTTGCCCACGATCCGGACGCCGGACTGTTCAGCACTCAGGGCTACACCCTGGTGCAACCGTCCGACCTGCCCAAGGACCCCGCAGGCCCGTTCCCCCAGTTGCGCTACCGGACTCCGGGGCCACTACCGTCACCGCTTTTGCCAGGCTCCTCGTCACCCCTGGAGCCTCTAGATCTGGTGCTGCTGCGGCGCTTGGACCGAGAGGAGGCGGCGGCGCACCGGCTGCAGATCGAGGCATGGGACGGCGGCCGACCCCGGCGCACCGGCCTCCTGAGCGTGGAGCTGCGCGTGCTGGATGAGAACGACAACCCGCCGGTCTTTGAGCAGGACGAGTCCCGCGCCGCGGTGCGAGAGCACGCCCAGCCGGGCGCCGAGGTCTGTCGCGTGCGCGCCACCGCCCGCGACGTGGGGCCCAATGGCTTCGTGCGCTACAGCGTCCGCGCCCGGCAAGTGCCTGGGGCGGGTAGCGGCGGCGGGGCACTGGGCGACGCGGCCTACTTCGCGTTGGTGGTGGAGGCCCGCGATGGAGGCGCCGAGCATGAGGTTGCCGCGGTGCGTGTGTCCATCGCCGTGCTGGACGTGAATGACAACCGGCCAGCAATTCACGTGCTCTTTCTCACAGAGGGAGGTGTCGCCCGTGTCTCTGAAGGCGCCCGCCCGGGCGACTACGTGGCTCGCGTCTCGGTGTCTGACGCGGGCGGTGACTGGGAGAAGGAAGATGAGGCCACAGGGGAGCTTGGTGTGACAGCCTCTGATGCAGATTCAGGACTCTATGGCTTTATTGAATATTCTCTTTATGATGGATTCCTGAGCTATGAAGCACCTCAGGCATTCCGGATCGACCCTCATGATGGGCAAATCTGTGTTTCTCAAGATATCGACAGGGAAAGGGATCCAGCTACCTATGATCTCCTGGTGGAAGCTAAGGATGGGTTTGAAATCATGCCAGGTGCTTCATTTGAATTATTCGAGATAAATTCTGACACTGGAGAGGTAGTGACAACCACCATACTTGACAGAGAAATTCAAGAAGTCTTCACCCTTCGAGTACTAGTACGAGATGGGGGATTCCCTTCATTGTCCAGCACCACAACAATCCTCTGCACTGTTGAAGATGAAAACGATCACGCACCAGAGTTTATTGTTTCCAGTTATGACATTGAGGTTCTGGAAAACCAGGAACCAGAGGTTGTCTATACGGTTTTAGCCTCTGATATGGATGCTGGCAATAACAGAGCTGTTGAATATCACATAATTGACTCCTCAGAACCAATCTTTTACAGGATTTCTTCTGGTGATCTCGGCGGAAAGTTCTCCATTCACCCGCGGCTGGGCACTATTCGCACCCGGAAGCCCCTGGATCACGAGACGCAGCCCGTGGTTGTGCTCACGGTGCAGGCGCAGCTCGGCAGCGCCCCAGCCTGCAGCAGCACCGAGGTCAACATAACAGTCATGGATGTCAATGACAACCACCCAGCGTTCCTCAGGACCTCGGATGAGATTAGAATATCCCAGACCACGCCCCCTGGCACAGCCTTGTACCTCGCACGTGCGGAAGACAGAGACAGTGGGCGGAACGGACTCATCCGGTACTCCATCGCCAGCCCGCAGCCAGGCGTCTTTGCCATCGACAGAGCCCTGGGGGTGCTGTTCCTCAACGGCAGCCTGGGCGCGGGCGAGCAGCGGGAGCTCACGCTGACTCTCAGGGCCGAGGACCAAGGCGTGCATCCTCAGGCAGCCCTGCTGGTGCTGACAGTCGTTATCGAGAAACGCGAACACAGCCCATCCTGGACTTTCGAACATTTGGTCTATCAAGTGGAAGTCAGTACTTCTATTGTGACTGTTAAAGCTTTTGCTCCTGACTCAATTCAGGACAGCATGAAATATTCAATTTTTAGTGGAAATGAAGATGGAGTTCTTTCCCTGTGCTCTAAGTCAGGTGTGGTGAACTGCCTTGCTTCTCTCAGTCACACAGACTTTCTCTCCCTGAAATTTGAATCTTCGGTGAAGGGACACCAAGACAGAGACAAATTACAGCCAATTCATCTTGATGACAACAACTCAAAGAAGCTGTGCTTTACATTCCCTAGAGCCACTCAGGCTCTTGTATTCACTGGGCACTGTCTTTCTGATACATCTCTCCCCGGTTGGGTTTTTGCTACCGACTTGGACAGTGGTTTGAACGGCCTGATTGAGTATTCTATTCTGTCTGGCAACCAAGAAGAAGCATTCCAGATTGATGCACTGAGTGGTGTGATAACAACAAAAGCGATTCTAGATTACGAGCTCACCAGCTCCTACAGCTTGATTGTCCAAGCCACAGATAAAGGGATGCCCAGGCTTTCTAATACGACTGTAATCAAGGTACAGGTGACTGATATAAATGACAATGCCCCAGCTTTTCTCCCCTCTGAAGCAGTGGAAATTACAGAAGTCATGACTATTTCAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTGAATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATTGATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAATATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTCCGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACAGTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAAAGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCTAAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACTCGATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCCGAGAGCTCTTACTTTAGTGGAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAATCTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGACCATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAGAACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGTTATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATTCTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTACTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAGGAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGGTCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTAGAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAATTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTGTTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGCTGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGATTTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACTCATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCATGCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAAGACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAATTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGACAGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATTCTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAGAACCAATGGAAATATTTATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAAATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTTGTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCAATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATGATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCTTTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACTGACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATAAGTATCATGGAGAAGGATATTGTCAATCTGTACAGATACTCAAACTCCAGTGGCCACTGTTCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTACAGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATCCCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCCGAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACCACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGCATCCTGGCTGACGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGTGCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAATTATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCAAAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTTTTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGAATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTACCATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTGACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACACATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATATGAAACCACTGGGATGCCAAGTACCTGCTCACCATTGGTCATGAATGAATGAACAAAATGTTTTCAAGCCGGCAACTCGAGATTGGGCTCATTTTTATCTAAAAGCAAGTGATGTAATTTAGTTAGAGTTTTTAAAACTTCCCCATTAAAGTTTCTCCAATTTCAAAAAAAAAAAAAAAAAAAANOV50i, CG58567-06SEQ ID NO: 10823361 aaMW at 367309.1kDProtein SequenceMEKCGLKEEGSYADIDPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPFFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLLSVELRVLDENDNPPVFEQDEYRAAVREDAQPGAEVCRVPATDRDLGPNGFVRYSVRARQVPGAGSGGGALGDAAYFAVEELSGVVRVWRPLDREAQAWHQLVVEARDGGAEPEVATVRVSIAVLDVNDNRPAIHVLFLTEGGVARVSEGARPGDYVARVSVSDADGDWEKEDEATGELGVGLGDGSISLSLEGGEGDFALLPGGPPGVFFLCVEGPLDRESRDLYELLLVATDAGSPPLSTEETLLLRVADLNDQPPLFSQQHYKASVSEAAAPGTVVMWVSASDADEAGSDHAWLRYTVVQLSAPCNLGSLQSKMVHTAECGPSFAIDSESGAISTIRTLDREVQEAVELKVVAQDLGEPPLSATCLVSITVDDVNDNEPIFWRQVYNATIAEHAPVGHCFLQLISAQVASVKIKHKHKEIHEKHNLAYISCPAGTIYVITWADGAAAFSGTDFAFSSDELQAFVLKSLFCELGEGELINWVALDREHRGHHEMTVLVTDRGSPPRNATMAVYVSVTDINDNRPFFPQCLPGKELHVKVLEGQPVNMLVTTVFAKDPDEGNNAEVTYSVSSEDSSDHFKIDANNGEIRTTTILSYDYRPSYRMSVIATDQGVPPLQGQAVVNIQVIPLSKGRAIMSQNIRHLIIPENLKPTKIMSLIKSSDHLQQHYNGKLHFSIVADDKDGHFEIDSSTGDLFLSKELDYETTSHYLFRVITTDHSKNLSLSSTVFLSIDVEDQNDHSPSFQDELIVISVEENVPIGTLVYVFNAKDDDGSFLNSRIQYYIESHNPGTNPFLIHPSFGTLVTVSRLDRESIPTVILTVTASDQAVNVTDRRLRSLTAQIVILDVNDHNPTFISFPNAHVKEDVTVGSLVHHITAHDPDEGRNGKVTYSILSGNENMTFMLDESSGLLTTTCPLDYEMKTQHILTVLALDDGTPALSSSQTLTVTVLDVNDEAPVFKQHLYEASVKENQNPGEFVTRVEALDRDSGMRLNGDPDRELCAGGNPLGSRAPPGSRTPPEGGLSAQAFVRVDLEDVNDNHPVFNPSTYVTSISDETQPGTEIINVLATDQDSGIYGTVAYELIPGNVSSLFTIDSTTGIIYLTLPLSHLESTTLSLMVSAQDGGGLTAVINADVTIHIFQTTLAPAEFERPKYTFLVYEDVPEDSPIGTVKAREPLNPPRSSVIHLQVRVLDANDHSPSFPTLYYQSSVREDAEVGTVVLVLSAVDKDEGLNGQTEYFLTDEACGAFTIDPMSGTLKTSNTLDREARSQHTFSAVARDCSIQGSRSTTVIIKVYVTDVNDNDPVLEQNPFDVFLSPESPTNQTTVIVRADDLDLGPNGTVDSDDSPLLDDFHVHPDTGIIRTARRLDRERRDHYSFVAATLLGAVVQVEIRVNDVNDHSPRFPLDSLQLDVSELSPPGTAFRLPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPFFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLLSVELRVLDENDNPPVFEQDESRAAVREHAQPGAEVCRVRATARDVGPNGFVRYSVRARQVPGAGSGGGALGDAAYFALVVEARDGGAEHEVAAVRVSIAVLDVNDNRPAIHVLFLTEGGVARVSEGARPGDYVARVSVSDAGGDWEKEDEATGELGVTASDADSGLYGFIEYSLYDGFLSYEAPQAFRIDPHDGQICVSQDIDRERDPATYDLLVEAKDGFEIMPGASFELFEINSDTGEVVTTTILDREIQEVFTLRVLVRDGGFPSLSSTTTILCTVEDENDHAPEFIVSSYDTEVLENQEPEVVYTVLASDMDAGNNRAVEYHIIDSSEPIFYRISSGDLGGKFSTHPRLGTIRTRKPLDHETQPVVVLTVQAQLGSAPACSSTEVNITVMDVNDNHPAFLRTSDEIRISQTTPPGTALYLARAEDRDSGRNGLIRYSIASPQPGVFAIDRALGVLFLNGSLGAGEQRELTLTLRAEDQGVHPQAALLVLTVVIEKREHSPSWTFEHLVYQVEVSTSIVTVKAFAPDSIQDSMKYSIFSGNEDGVLSLCSKSGVVNCLASLSHTDFLSLKFESSVKGHQDRDKLQPIHLDDNNSKKLCFTFPRATQALVFTGHCLSDTSLPGWVFATDLDSGLNGLIEYSILSGNQEEAFQIDALSGVITTKAILDYELTSSYSLIVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITEVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTRFLVEASDGGNPDPRALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNRTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPIAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRYSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQI


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 50B.

TABLE 50BComparison of the NOV50 protein sequences.NOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h MEKCGLKEEGSYADIDPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPFFQLRYRTPGPLPNOV50i MEKCGLKEEGSYADIDPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPFFQLRYRTPGPLPNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h SPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLLSVELRVLDENDNNOV50i SPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLLSVELRVLDENDNNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h PPVFEQDEYRAAVREDAQPGAEVCRVRATDRDLGPNGFVRYSVRARQVPGAGSGGGALGDNOV50i PPVFEQDEYRAAVREDAQPGAEVCRVRATDRDLGPNGFVRYSVRARQVPGAGSGGGALGDNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h AAYFAVEELSGVVRVWRPLDREAQAWHQLVVEARDGGAEPEVATVRVSIAVLDVNDNRPANOV50i AAYFAVEELSGVVRVWRPLDREAQAWHQLVVEARDGGAEPEVATVRVSIAVLDVNDNRPANOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h IHVLFLTEGGVARVSEGARPGDYVARVSVSDADGVIGKITAIDMDS--------------NOV50i IHVLFLTEGGVARVSEGARPGDYVARVSVSDADGDWEKEDEATGELGVGLGDGSISLSLENOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h ------------------------------------------------------------NOV50i GGEGDFALLPGGPPGVFFLCVEGPLDRESRDLYELLLVATDAGSPPLSTEETLLLRVADLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h ---------------------------------------------------------GK-NOV50i NDQPPLFSQQHYKASVSEAAAPGTVVMWVSASDADEAGSDHAWLRYTVVQLSAPCNLGSLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h ------------------------------------------------------------NOV50i QSKMVHTAECGPSFAIDSESGAISTIRTLDREVQEAVELKVVAQDLGEPPLSATCLVSITNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h --------------------------------NG-QLLYFLLSD----------------NOV50i VDDVNDNEPIFWRQVYNATIAEHAPVGHCFLQLISAQVASVKIKHKHKEIHEKHNLAYISNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h ----------G------------------------KFFKMNPNTGELINWVALDREHRGHNOV50i CPAGTIYVITWADGAAAFSGTDFAFSSDELQAFVLKSLFCELGEGELINWVALDREHRGHNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h HEMTVLVTDRGSPPRNATMAVYVSVTDINDNRPFFPQCLPGKELHVKVLEGQPVNMLVTTNOV50i HEMTVLVTDRGSPPRNATMAVYVSVTDINDNRPFFPQCLPGKELHVKVLEGQPVNMLVTTNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VFAKDPDEGNNAEVTYSVSSEDSSDHFKIDANNGEIRTTTILSYDYRPSYRMSVIATDQGNOV50i VFAKDPDEGNNAEVTYSVSSEDSSDHFKIDANNGEIRTTTILSYDYRPSYRMSVIATDQGNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VPPLQGQAVVNIQEL---------------------------------------------NOV50i VPPLQGQAVVNIQVIPLSKGRAIMSQNIRHLIIPENLKPTKIMSLIKSSDHLQQHYNGKLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h --------D--------------------YETTSHYLFRVITTDHSKNLSLSSTVFLSIDNOV50i HFSIVADDKDGHFEIDSSTGDLFLSKELDYETTSHYLFRVITTDHSKNLSLSSTVFLSIDNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VEDQNDHSPSFQDELIVISVEENVPIGTLVYVFNAKDDDGSFLNSRIQYYIESHNPGTNPNOV50i VEDQNDHSPSFQDELIVISVEENVPIGTLVYVFNAKDDDGSFLNSRIQYYIESHNPGTNPNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h FLIHPSFGTLVTVSRLDRESIPTVILTVTASDQAVNVTDRRLRSLTAQIVILDVNDHNPTNOV50i FLIHPSFGTLVTVSRLDRESIPTVILTVTASDQAVNVTDRRLRSLTAQIVILDVNDHNPTNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h FISFPNAHVKEDVTVGSLVHHITAHDPDEGRNGKVTYSILSGNENMTFMLDESSGLLTTTNOV50i FISFPNAHVKEDVTVGSLVHHITAHDPDEGRNGKVTYSILSGNENMTFMLDESSGLLTTTNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h CPLDYEMKTQHILTVLALDDGTPALSSSQTLTVTVLDVNDEAPVFKQHLYEASVKENQNPNOV50i CPLDYEMKTQHILTVTALDDGTPALSSSQTLTVTVLDVNDEAPVFKQHLYEASVKENQNPNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h GEFVTRVEALDRDSVFLNTRELNMCFLAFYDAVFK----------NGGLSAQAFVRVDLENOV50i GEFVTRVEALDRDSGNRLNGDPDRELCAGGNPLGSRAPPGSRTPPEGGLSAQAFVRVDLENOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h DVNDNHPVFNPSTYVTSISDETQPGTEIINVLATDQDSGTYGTVAYELIPGNVSSLFTIDNOV50i DVNDNHPVFNPSTYVTSISDETQPGTEIINVLATDQDSGTYGTVAYELIPGNVSSLFTIDNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h STTGIIYLTLPLSHLESTTLSLNVSAQDGGGLTAVINADVTIHIFQTTLAPAEFERPKYTNOV50i STTGIIYLTLPLSHLESTTLSLNVSAQDGGGLTAVINADVTIHIFQTTLAPAEFERPKYTNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h FLVYEDVPEDSPIGTVKAREPLNPPRSSVIHLQVRVLDANDHSPSFPTLYYQSSVREDAENOV50i FLVYEDVPEDSPIGTVKAREPLNPPRSSVIHLQVRVLDANDHSPSFPTLYYQSSVREDAENOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VGTVVLVLSAVDKDEGLNGQTEYFLTDEACGAFTTDPMSGTLKTSNTLDREARSQHTFSANOV50i VGTVVLVLSAVDKDEGLNGQTEYFLTDEACGAFTIDPMSGTLKTSNTLDREARSQHTFSANOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VARDCSIQGSRSTTVIIKVYVTDVNDNDPVLEQNPFDVFLSPESPTNQTTVIVRADDLDLNOV50i VARDCSIQGSRSTTVIIKVYVTDVNDNDPVLEQNPFDVFLSPESPTNQTTVIVRADDLDLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h GPNGTVDSDDSPLLDDFHVHPDTGIIRTARRLDRERRDHYSFVAATLLGAVVQVEIRVNDNOV50i GPNGTVDSDDSPLLDDFNVHPDTGIIRTARRLDRERRDHYSFVAATLLGAVVQVEIRVNDNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------N0V50h VNDHSPRFPLDSLQLDVSELSPPGTAFRLPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPNOV50i VNDHSPRFPLDSLQLDVSELSPPGTAFRLPVAHDPDAGLFSTQGYTLVQPSDLPKDPAGPNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h FFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLNOV50i FFQLRYRTPGPLPSPLLPGSSSPLEPLDLVLLRRLDREEAAAHRLQIEAWDGGRPRRTGLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h LSVELRVLDENDNPPVFEQDESRAAVREHAQPGAEVCRVRATARDVGPNGFVRYSVRARQNOV50i LSVELRVLDENDNPPVFEQDESRAAVREHAQPGAEVCRVRATARDVGPNGFVRYSVRARQNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VPGAGSGGGALGDAAYFALVVEARDGGAEHEVAAVRVSIAVLDVNDNRPAIHVLFLTEGGNOV50i VPGAGSGGGALGDAAYFALVVEARDGGAEHEVAAVRVSIAVLDVNDNRPAIHVLFLTEGGNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h VARVSEGARPGDYVARVSVSDAGGDWEKEDEATGELGVTASDADSGLYGFIEYSLYDGFLNOV50i VARVSEGARPGDYVARVSVSDAGGDWEKEDEATGELGVTASDADSGLYGFIEYSLYDGFLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h SYEAPQAFRIDPHDGQICVSQDIDRERDPATYDLLVEAKDGFEIMPGASFELFEINSDTGNOV50i SYEAPQAFRIDPHDGQICVSQDIDRERDPATYDLLVEAKDGFEIMPGASFELFEINSDTGNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h EVVTTTILDREIQEVFTLRVLVRDGGFPSLSSTTTILCTVEDENDHAPEFIVSSYDIEVLNOV50i EVVTTTILDREIQEVFTLRVLVRDGGFPSLSSTTTILCTVEDENDHAPEFIVSSYDIEVLNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h ENQEPEVVYTVLASDMDAGNNRAVEYHIIDSSEPIFYRISSGDLGGKFSIHPRLGTIRTRNOV50i ENQEPEVVYTVLASDMDAGNNRAVEYHIIDSSEPIFYRISSGDLGGKFSIHPRLGTIRTRNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h KPLDHETQPVVVLTVQAQLGSAPACSSTEVNITVMDVNDNHPAFLRTSDEIRISQTTPPGNOV50i KPLDHETQPVVVLTVQAQLGSAPACSSTEVNITVMDVNDNHPAFLRTSDEIRISQTTPPGNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h TALYLARAEDRDSGRNGLIRYSIASPQPGVFAIDRALGVLFLNGSLGAGEQRELTLTLRANOV50i TALYLARAEDRDSGRNGLIRYSIASPQPGVFAIDRALGVLFLNGSLGAGEQRELTLTLRANOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h EDQGVHPQAALLVLTVVIEKREHSPSWTFEHLVYQVEVSTSIVTVKAFAPDSIQDSMKYSNOV50i EDQGVHPQAALLVLTVVIEKREHSPSWTFEHLVYQVEVSTSIVTVKAFAPDSIQDSMKYSNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h IFSGNEDGVLSLCSKSGVVNCLASLSHTDFLSLKFESSVKGHQDRDKLQPIHLDDNNSKKNOV50i IFSGNEDGVLSLCSKSGVVNCLASLSHTDFLSLKFESSVKGHQDRDKLQPIHLDDNNSKKNOV50a ------------------------------------------------------------NOV50b ------------------------------------------------------------NOV50c ------------------------------------------------------------NOV50d ------------------------------------------------------------NOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ------------------------------------------------------------NOV50h LCFTFPRATQALVFTGHCLSDTSLPGWVFATDLDSGLNGLIEYSILSGNQEEAFQIDALSNOV50i LCFTFPRATQALVFTGHCLSDTSLPGWVFATDLDSGLNGLIEYSILSGNQEEAFQIDALSNOV50a ------------------LIVQATDKGMPRLSNTTVIKVQVTDTNDNAPAFLPSEAVEITNOV50b ----------------------------------------------------------GSNOV50c ----------------------------------------------------------GSNOV50d ----------------------------------------------------------GSNOV50e ------------------------------------------------------------NOV50f ------------------------------------------------------------NOV50g ----------------------------------------------------------GSNOV50h GVITTKAILDYELTSSYSLIVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITNOV50i GVITTKAILDYELTSSYSLIVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITNOV50a EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50b EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50c EVMTISEDSLPGVIVTHVSVHDVDTNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50d EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50e ------------------------------------------------------------NOV50f EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50g EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50h EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50i EVMTISEDSLPGVIVTHVSVHDVDLNSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFNOV50a EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50b EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50c EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50d EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50e --MTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50f EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50g EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50h EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50i EEMTEYELLIQISDSVHYTEGALVVRVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSNOV50a ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTRFLVEASDGGNPDPRNOV50b ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRNOV50c ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRNOV50d ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRNOV50e ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRNOV50f ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRNOV50g ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRNOV50h ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTRFLVEASDGGNPDPRNOV50i ATDLESNENISYRILSSSKEFSIDPKNGTIFTISPVLLLDTISTTRFLVEASDGGNPDPRNOV50a ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50b ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50c ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50d ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50e ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50f ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50g ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50h ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50i ALTLVEIGIEDMNNYAPEFTVKSYNLSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIINOV50a SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50b SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50c SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50d SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50e SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50f SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50g SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50h SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50i SGNSQNNFHVETKFFHSEYPYKQVGYLVLLHSLDREASASHELVILASDSGCPPLSSTAVNOV50a ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50b ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50c ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEITYNIISGNEKNOV50d ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50e ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50f ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50g ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50h ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKNOV50i ISIQVLDVNDNPPNFSSLSYHTHVKESTPLGSHITVVSANDRDTGSHAETIYNIISGNEKNOV50a GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50b GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50c GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50d GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50e GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50f GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50g GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50h GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50i GHFYLEENTGVLYLIKPLDYEKMTKFTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMNOV50a FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50b FSSFSCIVPENLPISSTIRSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50c FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50d FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50e FSSFSCIVPENLPISSTICSTNALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50f FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50g FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50h FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50i FSSFSCIVPENLPISSTICSINALDFDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLNOV50a TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50b TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50c TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50d TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50e TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50f TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50g TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50h TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50i TGDIHAKQILDYENGNKYCLTVQAKDKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPNOV50a EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNRTNGNIYLIRALPLIKSQLNKNOV50b EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKNOV50c EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKNOV50d EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKNOV50e EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPSLSVNKTNGNIYLIPALPLIKSQLNKNOV50f EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNTYLIRALPLIKSQLNKNOV50g EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKNOV50h EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNRTNGNIYLIRALPLIKSQLNKNOV50i EKNKDRQLIGRVEASDADAGIDGVILYSLGTSSPFFSVNRTNGNIYLIRALPLIKSQLNKNOV50a EDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICNOV50b EDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICNOV50c EDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLTLICNOV50d EDTLEMKIIAHSPKSDSKFASCTVFVNVSVD-----------------------------NOV50e EDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLTLICNOV50f EDTLEMKIIAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICNOV50g EDTLEMKIIAHSPKSDSKFASCTVFVNVSVD-----------------------------NOV50h EDTLEMKTTAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICNOV50i EDTLEMKITAHSPKSDSKFASCTVFVNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICN0V50a ILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50b ILIVNILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50c ILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50d ------------------------------------------------------------NOV50e ILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50f ILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50g ------------------------------------------------------------NOV50h ILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50i ILIVMILRHKQKDTINNYEEKKTSSLDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPENOV50a WLSLISIMEKDIVNLYRYSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50b WLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50c WLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50d ------------------------------------------------------------NOV50e WLSLISIMEKDTVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50f WLSLISIMEKDIVNLYRHSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50g ------------------------------------------------------------NOV50h WLSLISIMEKDIVNLYRYSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50i WLSLISIMEKDIVNLYRYSNSSGHCSVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVNOV50a PDSGIPPDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50b PDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50c PDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50d ------------------------------------------------------------NOV50e PDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50f PDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50g ------------------------------------------------------------NOV50h PDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50i PDSGIPRDSDQLSCLSGETDVMVTAETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKRNOV50a EAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50b EAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50c EAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50d ------------------------------------------------------------NOV50e EAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50f EAKESTLADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50g ------------------------------------------------------------NOV50h EAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50i EAKESILADVRKESVFISGDQEVRCAALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVNOV50a FNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRNOV50b FNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRNOV50c FNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRNOV50d ------------------------------------------------------------NOV50e FNDIAKLKDEHLHNPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRNOV50f FNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRNOV50g ------------------------------------------------------------NOV50h FNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRNOV50i FNDIAKLKDEHLHMPGIPKEKKSFVFPPPLITAVAQPGIKAVPPRNPAVNLGQVPPKHPRNOV50a SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50b SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50c SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50d ------------------------------------------------------------NOV50e SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50f SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50g ------------------------------------------------------------NOV50h SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50i SPIPYHLGSLPEGMTPNFSPSLSLLTMQPPALSPLLREGELLGTHISGTCHELKAEDEVQNOV50a I--NOV50b IVDNOV50c IVDNOV50d ---NOV50e I--NOV50f I--NOV50g ---NOV50h I--NOV50i I--NOV50a (SEQ ID NO: 1066)NOV50b (SEQ ID NO: 1068)NOV50c (SEQ ID NO: 1070)NOV50d (SEQ ID NO: 1072)NOV50e (SEQ ID NO: 1074)NOV50f (SEQ ID NO: 1076)NOV50g (SEQ ID NO: 1078)NOV50h (SEQ ID NO: 1080)NOV50i (SEQ ID NO: 1082)


Further analysis of the NOV50a protein yielded the following properties shown in Table 50C.

TABLE 50CProtein Sequence Properties NOV50aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 8; pos. chg 1; neg. chg 1H-region:length 3; peak value −4.35PSG score:−8.75GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −9.63possible cleavage site: between 38 and 39>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 690-706−18.15PERIPHERALLikelihood =  2.54 (at 195)ALOM score: −18.15 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 697Charge difference: 3.5 C(2.5)-N(−1.0)C > N: C-terminal side will be inside>>> membrane topology: type 1b (cytoplasmic tail 690 to 1063)MITDISC:discrimination of mitochondrial targeting seqR content: 1Hyd Moment(75):8.18Hyd Moment(95):10.59G content:1D/E content: 2S/T content:5Score: −4.29Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 22 PRL|SNNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PKEKKSF (5) at 960bipartite: nonecontent of basic residues: 7.1%NLS Score: −0.04KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 929LL at 1026LL at 1037LL at 1043checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: nuclear26.1%: cytoplasmic17.4%: mitochondrial 8.7%: vesicles of secretory system 4.3%: vacuolar 4.3%: peroxisomal 4.3%: endoplasmic reticulum>> prediction for CG58567-01 is nuc (k = 23)


A search of the NOV50a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 50D.

TABLE 50DGeneseq Results for NOV50aIdentities/NOV50aSimilaritiesGeneseqProtein/Organism/LengthResidues/for theExpectIdentifier[Patent #, Date]Match ResiduesMatched RegionValueABP70112Human NOV28c - Homo sapiens,1 . . . 10631063/1063(100%)0.03361 aa. [WO200272771-A2,2299 . . . 3361  1063/1063(100%)19-SEP-2002]ABP70111Human NOV28b - Homo sapiens,1 . . . 10631063/1063(100%)0.03003 aa. [WO200272771-A2,1941 . . . 3003  1063/1063(100%)19-SEP-2002]ABP70110Human NOV28a - Homo sapiens,1 . . . 10631063/1063(100%)0.01063 aa. [WO200272771-A2,1 . . . 10631063/1063(100%)19-SEP-2002]ABP43938FLJ20047 fis clone COL00577 -105 . . . 1063 959/959(100%)0.0Homo sapiens, 959 aa.1 . . . 959 959/959(100%)[WO200231111-A2, 18-APR-2002]AAO26793Human cadherin (CAD) protein, SEQ1 . . . 1036366/1073(34%)e−148ID No 16 - Homo sapiens, 3298 aa.2245 . . . 3270  543/1073(50%)[WO200299042-A2, 12-DEC-2002]


In a BLAST search of public sequence databases, the NOV50a protein was found to have homology to the proteins shown in the BLASTP data in Table 50E.

TABLE 50EPublic BLASTP Results for NOV50aNOV50aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ9NXU8Hypothetical protein FLJ20047 - 105 . . . 1063959/959(100%)0.0Homo sapiens (Human), 959 aa. 1 . . . 959959/959(100%)Q9NRU0Cadherin-like protein VR8 - Homo189 . . . 582391/394(99%)0.0sapiens (Human), 398 aa (fragment). 5 . . . 398393/394(99%)Q96JQ0Protocadherin 16 precursor  1 . . . 1036366/1073(34%)e−148(Cadherin 19) (Cadherin fibroblast2245 . . . 3270543/1073(50%)1) - Homo sapiens (Human), 3298 aa.Q24292Dachsous protein precursor  1 . . . 1037328/1165(28%)e−100(Adherin) - Drosophila melanogaster2343 . . . 3471528/1165(45%)(Fruit fly), 3503 aa.Q8AXB7Protocadherin - Brachydanio rerio 3 . . . 605206/615(33%)3e−77(Zebrafish) (Danio rerio), 4610 aa.2885 . . . 3471317/615(51%)


PFam analysis predicts that the NOV50a protein contains the domains shown in the Table 50F.

TABLE 50FDomain Analysis of NOV50aIdentities/PfamNOV50aSimilarities forExpectDomainMatch Regionthe Matched RegionValuecadherin 43 . . . 13026/108 (24%)1.6e−1464/108 (59%)cadherin144 . . . 23236/108 (33%)2.5e−1266/108 (61%)cadherin246 . . . 34836/113 (32%)  2e−1579/113 (70%)cadherin362 . . . 45235/107 (33%)8.4e−2475/107 (70%)cadherin466 . . . 56333/115 (29%)1.1e−1167/115 (58%)cadherin577 . . . 67126/110 (24%)0.1760/110 (55%)


Example 51

The NOV51 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 51A.

TABLE 51 ANOV51 Sequence AnalysisNOV51 a, CG59534-03SEQ ID NO:10833333 bpDNA SequenceORF Start: ATG at 15ORF Stop: TAG at 3219GCGCTCCAGACAAGATGGCGCGGCCGGTCCGGGGAGGGCTCGGGGCCCCGCGCCGCTCGCCTTGCCTTCTCCTTCTCTGGCTGGTTTTGGTTCGGCTGGAGCCGGTGACCGCCGCGGCCGGCCCGCGGGCGCCCTGCGCGGCCGCCTGCACTTGCGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCTGCGTTGCCCGGGGACCTGCCCTCCTGGACGCGGAGCCTAAACCTGAGTTACAACAAACTCTCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACCTACAGGAAGTGCAGCACAACAAGATTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCCTTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCACACGGACCGCCTATAAAGGAGCTCAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTGGGAGCATTTGATGGTCTGTCACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATCACCCAGCTTCCTGTAAGAGCATTCAAGCTACCCAGGCTGACACAACTGGACCTCAATCGGAACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGCTCAACAGCTTGGAGGTGCTGAAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGGACTGTCCAAGATGCATGTGCTGCACCTGGAGTACAACAGATTCCATCGCTCGCATTCACCGCAAGGGCTGGAGCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACACGGCTGGACGAGGAGAGCCTGGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCACAATTCCATCAGCCACATTGCGGAGGGTGCCTTCAAGGGACTCAGGAGCCTGCGAGTCTTGGATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACACGAGCGGCGCCTTCTCAGGGCTCGACAGCCTCAGCAAGCTGACTCTGTTTGGAAACAAGATCAAGTCTGTGGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAATGCGATCAGATCTGTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCATATCAGCAGCGACAGCTTCCTGTGTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATTGGCAGGATGCTGCAGGCCTTTGTGACAGCCACCTGTGCCCACCCAGAATCACTGAAGGGTCAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGATGACTTCCTGAAGCCACAGATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGTTTACATGCTCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTCCTGACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATGGAGTACACCACCATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAATGTGTCATCACCAACCACTTTGGCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAATGTGTTGCCATCATTCACCAAAACGCCCCACGACATAACCATCCGGACCACCACCGTGGCCCGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATTGCCTGGCAGAAGGATGGAGGCACGGATTTCCCCGCTGCCCGTGAGCGACGCATGCATGTCATGCCGGATGACGACGTGTTTTTCATCACTGATGTGAAAATAGATGACGCAGGGGTTTACAGCTGTACTGCTCAGAACTCAGCCGGTTCTATTTCAGCTAATGCCACCCTGACTGTCCTAGAGACCCCATCCTTGGTGGTCCCCTTGGAAGACCGTGTGGTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGCATCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACCTGACCCCTGACAACCAGCTCCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGTGAGATGTCCAACACCCTGGGCACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCAGCAGGCTGCAGGAAGGATGGGACCACGGTAGGCATCTTCACCATTGCTGTCGTGAGCAGCATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCAGACCAGGAAGAAGAGTGAAGAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCAAGCTACCTCTCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCTCAGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCAGAGCCCGACACTCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTATCACAAAGAGCCGTGGAAAGCGATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATGGAACACGGTGGCCGGGTCGTATGCAGTGACTGCAACACCGAAGTGGACTGTTACTCCAGGGGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGCACAGCCAAGTGCGCCAAATGGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGCAGCAGGACTGCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATGCTGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGGACTTTAGCAAGGTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACTTCAGGCAGTCCAGAGCGCGCGGAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCCAAAGCATGTGACGCCAGTCCCGAGTCCACGCCACTGACAGGACAGCTCCCCGGGAAACAGAGGGTGCCACTGCTGTTGGCACCAAAAAGCTAGGTTTTGTCTACCTCAGTTCTTGTCATACCAATCTCTACGGGAAAGAGAGGTAGGAGAGGCTGCGAGGAAGCTTGGGTTCAAGCGTCACTCATCTGTACATAGTTGTAACTCCNOV51a, CG59534-03SEQ ID NO:10841068 aaMW at 116452.8 kDProtein SequenceMARPVRGGLGAPRRSPCLLLLWLVLVRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCANPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTVARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSPDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKSNOV51b, CG59534-01SEQ ID NO:10855101 bpDNA SequenceORF Start: ATG at 77ORF Stop: TAG at 3308ATCCTCCTACCTCAGTCTACTGAGTAGCTAGGACTACAGGCATGTGCCACCACACCTGGCTAGTTTTTATTTTTTGATGAGATGGGATCTTGCTGTGTTGCCCAGACTGGTCTTGATCTCCTGGGCTCAAGTGATCCTCCTGCCTTGGCCTGCGGCCGCCTGCACTTGCGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCTGCGTTGCCCGGGGACCTGCCCTCCTGGACGCGGAGCCTAAACCTGAGTTACAACAAACTCTCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACCTACAGGAAGTGTACCTCAATAATAATGAGTTGTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCCTTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCACACGGACCGCCTATAAAGGAGCTGAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTGGGAGCATTTGATGGTCTGTCACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATCACCCAGCTTCCTGTAAGAGCATTCAAGCTACCCAGGCTGACACAACTGGACCTCAATCGGAACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGCTCAACAGCTTGGAGGTGCTGAAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGGACTGTCCAAGATGCATGTGCTGCACCTGGAGTACAACAGCCTGGTAGAAGTGAACAGCGGCTCGCTCTACGGCCTCACGGCCCTGCATCAGCTCCACCTCAGCAACAATTCCATCGCTCGCATTCACCGCAAGGGCTGGAGCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACACGGCTGGACGAGGAGAGCCTGGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCACAATTCCATCAGCCACATTGCGGAGGGTGCCTTCAAGGGACTCAGGAGCCTGCGAGTCTTGGATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACACGAGCGGCGCCTTCTCAGGGCTCGACAGCCTCAGCAAGCTGACTCTGTTTGGAAACAAGATCAAGTCTGTGGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAATGCGATCAGATCTGTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCATATCAGCAGCGACAGCTTCCTGTGTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATTGGCAGGATGCTGCAGGCCTTTGTGACAGCCACCTGTGCCCACCCAGAATCACTGAAGGGTCAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGATGACTTCCTGAAGCCACAGATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGTTTACATGCTCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTCCTGACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATGGAGTACACCACCATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAATGTGTCATCACCAACCACTTTGGCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAATGTGTTGCCATCATTCACCAAAACGCCCCACGACATAACCATCCGGACCACCACCATGGCCCGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATTGCCTGGCAGAAGGATGGAGGCACGGATTTCCCCGCTGCCCGTGAGCGACGCATGCATGTCATGCCGGATGACGACGTGTTTTTCATCACTGATGTGAAAATAGATGACGCAGGGGTTTACAGCTGTACTGCTCAGAACTCAGCCGGTTCTATTTCAGCTAATGCCACCCTGACTGTCCTAGAGACCCCATCCTTGGTGGTCCCCTTGGAAGACCGTGTGGTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGCATCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACCTGACCCCTGACAACCAGCTCCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGTGAGATGTCCAACACCCTGGGCACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCAGCAGGCTGCAGGAAGGATGGGACCACGGTAGGCATCTTCACCATTGCTGTCGTGAGCAGCATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCAGACCAGGAAGAAGAGTGAAGAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCAAGCTACCTCTCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCTCAGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCAGAGCCCGACACTCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTATCACAAAGAGCCGTGGAAAGCGATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATGGAACACGGTGGCCGGGTCGTATGCAGTGACTGCAACACCGAAGTGGACTGTTACTCCAGGGGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGCACAGCCAAGTGCGCCAAATGGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGCAGCAGGACTGCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATGCTGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGGACTTTAGCAAGGTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACTTCAGGCAGTCCAGAGCGCGCGGAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCCAAAGCATGTGACGCCAGTCCCGAGTCCACGCCACTGACAGGACAGCTCCCCGGGAAACAGAGGGTGCCACTGCTGTTGGCACCAAAAAGCTAGGTTTTGTCTACCTCAGTTCTTGTCATACCAATCTCTACGGGAAAGAGAGGTAGGAGAGGCTGCGAGGAAGCTTGGGTTCAAGCGTCACTCATCTGTACATAGTTGTAACTCCCATGTGGAGTATCAGTCGCTCACAGGACTTGGATCTGAAGCACAGTAAACGCAAGAGGGGATTTGTGTACAAAAGGCAAAAAAAAGTATTTGATATCATTGTACATAAGAGTTTTCAGAGATTTCATATATATCTTTTACAGAGGCTATTTTAATCTTTAGTGCATGGTTAACAGAAAAAAATTATACAATTTTGACAATATTATTTTTCGTATCAGGTTGCTGTTTAATTTTGGAGGGGGTGGGGAAATAGTTCTGGTGCCTTAACGCATGGCTGGAATTTATAGAGGCTACAACCACATTTGTTCACAGGAGTTTTTGGTGCGGGGTGGGAAGGATGGAAGGCCTTGGATTTATATTGCACTTCATAGACCCCTAGGCTGCTGTGCGGTGGGACTCCACATGCGCCGGAAGGAGCTTCAGGTGAGCACTGCTCATGTGTGGATGCCCCTGCAACAGGCTTCCCTGTCTGTAGAGCCAGGGGTGCAAGTGCCATCCACACTTGCAGTGAATGGCTTTTCCTTTTAGGTTTAAGTCCTGTCTGTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTGTTAATCCATCACAAGCAAAAGGTCAGAACAGTTAAACACTGCCTTTCCTCCTCCTCTTATTTTATGATAAAAGCAAATGTGGCCTTCTCAGTATCATTCGATTGCTATTTGAGACTTTTAAATTAAGGTAAAGGCTGCTGGTGTTGGTACCTGTGGATTTTTCTATACTGATGTTTTCGTTTTGCCAATATAATGAGTATTACATTGGCCTTGGGGGACAGAAAGGAGGAAGTTCTGACTTTTCAGGGCTACCTTATTTCTACTAAGGACCCAGAGCAGGCCTGTCCATGCCATTCCTTCGCACAGATGAAACTGAGCTGGGACTGGAAAGGACAGCCCTTGACCTGGGTTCTGGGTATAATTTGCACTTTTGAGACTGGTAGCTAACCATCTTATGAGTGCCAATGTGTCATTTAGTAAAACTTAAATAGAAACAAGGTCCTTCAAATGTTCCTTTGGCCAAAAGCTGAAGGGAGTTACTGAGAAAATAGTTAACAATTACTGTCAGGTGTCATCACTGTTCAAAAGGTAAGCACATTTAGAATTTTGTTCTTGACAGTTAACTGACTAATCTTACTTCCACAAAATATGTGAATTTGCTGCTTCTGAGAGGCAATGTGAAAGAGGGAGTATTACTTTTATGTACAAAGTTATTTATTTATAGAAATTTTGGTACAGTGTACATTGAAAACCATGTAAAATATTGAAGTGTCTAACAAATGGCATTGAAGTGTCTTTAATAAAGGTTCATTTATAAATGTCAGTATAGTTGGTGGTCCTTCTTTTACAAACGCAGTCATTCTGCCTTTAATTATCTTCCCCCAAAAAAGAAAAAAAAAATAGGCGAAGCAAAATCACATACTGTTTGTTTGCTCCAGGGCAGACAACACTGCTAGATTCCTGACATTTTGTTTTGAATTTTTCTACACCTGGAGCTTGTTAGTCAAGGTCTAAAATCCCTAAGTGTGGTGACCTTTCCATTTCATCCTGCCTTTTCAAAGCTGGCCCAGGCCCTCCTTTCAGTCTGACATGAGAATGGCGAGAATGGCTCACCCACCGTGCCCTCCTGCACGAAGCCAGCTGGGCCNOV51b, CG59534-01SEQ ID NO:10861077 aaMW at 117735.2 kDProtein SequenceMRWDLAVLPRLVLISWAQVILLPWPAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKNHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKSNOV51c, CG59534-02SEQ ID NO:10874762 bpDNA SequenceORF Start: ATG at 15ORF Stop: TAG at 3294GCGCTCCAGACAAGATGGCGCGGCCGGTCCGGGGAGGGCTCGGGGCCCCGCGCCGCTCGCCTTGCCTTCTCCTTCTCTGGCTGGTTTTGGTTCGGCTGGAGCCGGTGACCGCCGCGGCCGGCCCGCGGGCGCCCTGCGCGGCCGCCTGCACTTGCGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCTGCGTTGCCCGGGGACCTGCCCTCCTGGACGCGGAGCCTAAACCTGAGTTACAACAAACTCTCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACCTACAGGAAGTGTACCTCAATAATAATGAGTTGACAGCGGTACCATCCCTGGGCGCTGCTTCATCACATGTCGTCTCTCTCTTTCTGCAGCACAACAAGATTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCCTTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCACACGGACCGCCTATAAAGGAGCTCAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTGGGAGCATTTGATGGTCTGTCACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATCACCCAGCTTCCTGTAAGAGCATTCAAGCTACCCAGGCTGACACAACTGGACCTCAATCGGAACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGCTCAACAGCTTGGAGGTGCTGAAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGGACTGTCCAAGATGCATGTGCTGCACCTGGAGTACAACAGCCTGGTAGAAGTGAACAGCGGCTCGCTCTACGGCCTCACGGCCCTGCATCAGCTCCACCTCAGCAACAATTCCATCGCTCGCATTCACCGCAAGGGCTGGAGCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACACGGCTGGACGAGGAGAGCCTGGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCACAATTCCATCAGCCACATTGCGGAGGGTGCCTTCAAGGGACTCAGGAGCCTGCGAGTCTTGGATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACACGAGCGGCGCCTTCTCAGGGCTCGACAGCCTCAGCAAGCTGACTCTGTTTGGAAACAAGATCAAGTCTGTGGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAATGCGATCAGATCTGTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCATATCAGCAGCGACAGCTTCCTGTGTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATTGGCAGGATGCTGCAGGCCTTTGTGACAGCCACCTGTGCCCACCCAGAATCACTGAAGGGTCAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGATGACTTCCTGAAGCCACAGATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGTTTACATGCTCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTCCTGACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATGGAGTACACCACCATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAATGTGTCATCACCAACCACTTTGGCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAATGTGTTGCCATCATTCACCAAAACGCCCCACGACATAACCATCCGGACCACCACCGTGGCCCGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATTGCCTGGCAGAAGGATGGAGGCACGGATTTCCCCGCTGCCCGTGAGCGACGCATGCATGTCATGCCGGATGACGACGTGTTTTTCATCACTGATGTGAAAATAGATGACGCAGGGGTTTACAGCTGTACTGCTCAGAACTCAGCCGGTTCTATTTCAGCTAATGCCACCCTGACTGTCCTAGAGACCCCATCCTTGGTGGTCCCCTTGGAAGACCGTGTGGTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGCATCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACCTGACCCCTGACAACCAGCTCCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGTGAGATGTCCAACACCCTGGGCACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCAGCAGGCTGCAGGAAGGATGGGACCACGGTAGGCATCTTCACCATTGCTGTCGTGAGCAGCATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCAGACCAGGAAGAAGAGTGAAGAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCAAGCTACCTCTCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCTCAGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCAGAGCCCGACACTCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTATCACAAAGAGCCGTGGAAAGCGATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATGGAACACGGTGGCCGGGTCGTATGCAGTGACTGCAACACCGAAGTGGACTGTTACTCCAGGGGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGCACAGCCAAGTGCGCCAAATGGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGCAGCAGGACTGCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATGCTGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGGACTTTAGCAAGGTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACTTCAGGCAGTCCAGAGCGCGCGGAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCCAAAGCATGTGACGCCAGTCCCGAGTCCACGCCACTGACAGGACAGCTCCCCGGGAAACAGAGGGTGCCACTGCTGTTGGCACCAAAAAGCTAGGTTTTGTCTACCTCAGTTCTTGTCATACCAATCTCTACGGGAAAGAGAGGTAGGAGAGGCTGCGAGGAAGCTTGGGTTCAAGCGTCACTCATCTGTACATAGTTGTAACTCCCATGTGGAGTATCAGTCGCTCACAGGACTTGGATCTGAAGCACAGTAAACGCAAGAGGGGATTTGTGTACAAAAGGCAAAAAAAGTATTTGATATCATTGTACATAAGAGTTTTCAGAGATTTCATATATATCTTTTACAGAGGCTATTTTAATCTTTAGTGCATGGTTAACAGAAAAAAATTATACAATTTTGACAATATTATTTTTCGTATCAGGTTGCTGTTTAATTTTGGAGGGGGTGGGGAAATAGTTCTGGTGCCTTAACGCATGGCTGGAATTTATAGAGGCTACAACCACATTTGTTCACAGGAGTTTTTGGTGCGGGGTGGGAAGGATGGAAGGCCTTGGATTTATATTGCACTTCATAGACCCCTAGGCTGCTGTGCGGTGGGACTCCACATGCGCCGGAAGGAGCTTCAGGTGAGCACTGCTCATGTGTGGATGCCCCTGCAACAGGCTTCCCTGTCTGTAGAGCCAGGGGTGCAAGTGCCATCCACACTTGCAGTGAATGGCTTTTCCTTTTAGGTTTAAGTCCTGTCTGTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTGTTAATCCATCACAAGCAAAAGGTCAGAACAGTTAAACACTGCCTTTCCTCCTCCTCTTATTTTATGATAAAAGCAAATGTGGCCTTCTCAGTATCATTCGATTGCTATTTGAGACTTTTAAATTAAGGTAAAGGCTGCTGGTGTTGGTACCTGTGGATTTTTCTATACTGATGTTTTCGTTTTGCCAATATAATGAGTATTACATTGGCCTTGGGGGACAGAAAGGAGGAAGTTCTGACTTTTCAGGGCTACCTTATTTCTACTAAGGACCCAGAGCAGGCCTGTCCATGCCATTCCTTCGCACAGATGAAACTGAGCTGGGACTGGAAAGGACAGCCCTTGACCTGGGTTCTGGGTATAATTTGCACTTTTGAGACTGGTAGCTAACCATCTTATGAGTGCCAATGTGTCATTTAGTAAAACTTAAATAGAAACAAGGTCCTTCAAATGTTCCTTTGGCCAAAAGCTGAAGGGAGTTACTGAGAAAATAGTTAACAATTACTGTCAGGTGTCATCACTGTTCAAAAGGTAAGCACATTTAGAATTTTGTTCTTGACAGTTAACTGACTAATCTTACTTCCACAAAATATGTGAATTTGCTGCTTCTGAGAGGCAATGTGAAAGAGGGAGTATTACTTTTATGTACAAAGTTATTTATTTATAGAAATTTTGGTACAGTGTACATTGAAAACCATGTAAAATATTGAAGTGTCTAACAAATGGCATTGAAGTGTCTTTAATAAAGGTTCATTTATAAATGTCANOV51c, CG59534-02SEQ ID NO:10881093 aaMW at 119051.7 kDProtein SequenceMARPVRGGLGAPRRSPCLLLLWLVLVRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVEAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKAPLTVNVLPSFTKTPHDITIRTTTVARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLILAPKSNOV51d, SNP13377255 ofSEQ ID NO:10893333 bpCG59534-03, DNA SequenceORF Start: ATG at 15ORF Stop: TAG at 3219SNP Pos: 1912SNP Change: T to CGCGCTCCAGACAAGATGGCGCGGCCGGTCCGGGGAGGGCTCGGGGCCCCGCGCCGCTCGCCTTGCCTTCTCCTTCTCTGGCTGGTTTTGGTTCGGCTGGAGCCGGTGACCGCCGCGGCCGGCCCGCGGGCGCCCTGCGCGGCCGCCTGCACTTGCGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCTGCGTTGCCCGGGGACCTGCCCTCCTGGACGCGGAGCCTAAACCTGAGTTACAACAAACTCTCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACCTACAGGAAGTGCAGCACAACAAGATTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCCTTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCACACGGACCGCCTATAAAGGAGCTCAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTGGGAGCATTTGATGGTCTGTCACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATCACCCAGCTTCCTGTAAGAGCATTCAAGCTACCCAGGCTGACACAACTGGACCTCAATCGGAACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGCTCAACAGCTTGGAGGTGCTGAAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGGACTGTCCAAGATGCATGTGCTGCACCTGGAGTACAACAGCCTGGTAGAAGTGAACAGCGGCTCGCTCTACGGCCTCACGGCCCTGCATCAGCTCCACCTCAGCAACAATTCCATCGCTCGCATTCACCGCAAGGGCTGGAGCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACACGGCTGGACGAGGAGAGCCTGGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCACAATTCCATCAGCCACATTGCGGAGGGTGCCTTCAAGGGACTCAGGAGCCTGCGAGTCTTGGATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACACGAGCGGCGCCTTCTCAGGGCTCGACAGCCTCAGCAAGCTGACTCTGTTTGGAAACAAGATCAAGTCTGTGGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAATGCGATCAGATCTGTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCATATCAGCAGCGACAGCTTCCTGTGTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATTGGCAGGATGCTGCAGGCCTTTGTGACAGCCACCTGTGCCCACCCAGAATCACTGAAGGGTCAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGATGACTTCCTGAAGCCACAGATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGTTTACATGCTCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTCCTGACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATGGAGTACACCACCATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAATGTGTCATCACCAACCACTTTGGCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAATGTGTTGCCATCATTCACCAAAACGCCCCACGACATAACCATCCGGACCACCACCGTGGCCCGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATTGCCTGGCAGAAGGATGGAGGCACGGATTTCCCCGCTGCCCGTGAGCGACGCATGCATGTCATGCCGGATGACGACGTGTTTTCCATCACTGATGTGAAAATAGATGACGCAGGGGTTTACAGCTGTACTGCTCAGAACTCAGCCGGTTCTATTTCAGCTAATGCCACCCTGACTGTCCTAGAGACCCCATCCTTGGTGGTCCCCTTGGAAGACCGTGTGGTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGCATCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACCTGACCCCTGACAACCAGCTCCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGTGAGATGTCCAACACCCTGGGCACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCAGCAGGCTGCAGGAAGGATGGGACCACGGTAGGCATCTTCACCATTGCTGTCGTGAGCAGCATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCAGACCAGGAAGAAGAGTGAAGAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCAAGCTACCTCTCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCTCAGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCAGAGCCCGACACTCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTATCACAAAGAGCCGTGGAAAGCGATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATGGAACACGGTGGCCGGGTCGTATGCAGTGACTGCAACACCGAAGTGGACTGTTACTCCAGGGGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGCACAGCCAAGTGCGCCAAATGGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGCAGCAGGACTGCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATGCTGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGGACTTTAGCAAGGTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACTTCAGGCAGTCCAGAGCGCGCGGAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCCAAAGCATGTGACGCCAGTCCCGAGTCCACGCCACTGACAGGACAGCTCCCCGGGAAACAGAGGGTGCCACTGCTGTTGGCACCAAAAAGCTAGGTTTTGTCTACCTCAGTTCTTGTCATACCAATCTCTACGGGAAAGAGAGGTAGGAGAGGCTGCGAGGAAGCTTGGGTTCAAGCGTCACTCATCTGTACATAGTTGTAACTCCNOV51d, SNP13377255 ofSEQ ID NO:10901068 aaMW at 116392.7 kDCG59534-03, Protein SequenceSNP Pos: 633SNP Change: Phe to SerMARPVRGGLGAPRRSPCLLLLWLVLVRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGAFKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRSVQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCDDFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTTILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTVARLECAATGHPNPQIAWQKDGGTDFPAARERRMHVMPDDDVFSITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLEDRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLGTERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVPSYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKAMEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQCSRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERAEAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 51B.

TABLE 51BComparison of the NOV51 protein sequences.NOV51aMARPVRGGLGAPRRSPCLLLLWLVLVRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLA(SEQ ID NO:1084)NOV51b----MRWDLAVLPR--------LVLISWAQVILLPWP----AAACTCAGDSLDCGGRGLA(SEQ ID NO:1086)NOV51cMARPVRGGLGAPRRSPCLLLLWLVLVRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLA(SEQ ID NO:1088)NOV51aALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLGEV-----------------------NOV51bALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLNOV51cALPGDLPSWTRSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLNOV51a--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


Further analysis of the NOV51a protein yielded the following properties shown in Table 51C.

TABLE 51CProtein Sequence Properties NOV51aSignalP analysis:Cleavage site between residues 36 and 37PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos.chg 2; neg.chg 0H-region:length 6; peak value −9.10PSG score:−13.50GvH: von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.07possible cleavage site: between 33 and 34>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2INTEGRALLikelihood =Transmembrane 17-33−2.87INTEGRALLikelihood =Transmembrane 773-789−8.23PERIPHERALLikelihood = 3.76 (at 656)ALOM score: −8.23 (number of TMSs: 2)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 24Charge difference: −4.0 C(1.0)-N(5.0)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC:discrimination of mitochondrial targeting seqR content:5Hyd Moment(75):4.80Hyd Moment(95):12.52G content:3D/E content:1S/T content:1Score: −0.99Gavel: prediction of cleavage sites for mitochondrial preseqR-2 motif at 48 PRA|PCNUCDISC: discrimination of nuclear localization signalspat4: KKKP (4) at 983pat7: PAARERR (3) at 616bipartite: nonecontent of basic residues: 9.7%NLS Score: 0.04KDEL: ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: ARPVKKXX-like motif in the C-terminus: LAPKSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs:Leucine zipper pattern (PS00029): *** found *** LDCGGRGLAALPGDLPSWTRSL at 52nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: nuclear30.4%: mitochondrial21.7%: endoplasmic reticulum 4.3%: plasma membrane 4.3%: cytoplasmic 4.3%: peroxisomal>> prediction for CG59534-03 is nuc (k = 23)


A search of the NOV51a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 51D.

TABLE 51DGeneseq Results for NOV51aNOV51aIdentities/GeneseqProtein/Organism/LengthResidues/MatchSimilarities for theExpectIdentifier[Patent #, Date]ResiduesMatched RegionValueABG69660Human secreted protein SCEP-40 -1 . . . 10681065/1093 (97%)0.0Homo sapiens, 1093 aa.1 . . . 10931068/1093 (97%)[WO200248337-A2, 20-JUN-2002]AAY08008Human HLIG-1 protein - Homo sapiens,1 . . . 10681053/1101 (95%)0.01101 aa. [EP913472-A2,1 . . . 11011062/1101 (95%)06-MAY-1999]ABG69674Human secreted protein SCEP-54 -1 . . . 10681042/1093 (95%)0.0Homo sapiens, 1070 aa.1 . . . 10701045/1093 (95%)[WO200248337-A2, 20-JUN-2002]ABP70116Human NOV30a - Homo sapiens,42 . . . 1068 1026/1052 (97%)0.01077 aa. [WO200272771-A2,26 . . . 1077 1027/1052 (97%)19-SEP-2002]AAY97833Murine LIG-1 protein - Mus sp, 10911 . . . 1068 887/1102 (80%)0.0aa. [JP2000093043-A, 04-APR-2000]1 . . . 1091 945/1102 (85%)


In a BLAST search of public sequence databases, the NOV51 a protein was found to have homology to the proteins shown in the BLASTP data in Table 51E.

TABLE 51EPublic BLASTP Results for NOV51aProteinNOV51aIdentities/AccessionResidues/MatchSimilarities for theExpectNumberProtein/Organism/LengthResiduesMatched PortionValueQ96JA1Membrane glycoprotein LIG-1 -1 . . . 10681068/1093(97%)0.0Homo sapiens (Human), 1093 aa.1 . . . 10931068/1093(97%)Q9BYB8Membrane glycoprotein LIG-1 -1 . . . 10681064/1094(97%)0.0Homo sapiens (Human), 1094 aa.1 . . . 10941068/1094(97%)P70193Membrane glycoprotein -1 . . . 1068887/1102(80%)0.0Mus musculus (Mouse), 1091 aa.1 . . . 1091945/1102(85%)Q9UFI4Hypothetical protein -586 . . . 1068481/483(99%)0.0Homo sapiens (Human), 483 aa1 . . . 483481/483(99%)(fragment).O94898Hypothetical protein KIAA0806 -41 . . . 993491/1002(49%)0.0Homo sapiens (Human), 1065 aa.43 . . . 991624/1002(62%)


PFam analysis predicts that the NOV51a protein contains the domains shown in the Table 51F.

TABLE 51FDomain Analysis of NOV51aIdentities/SimilaritiesPfamNOV51afor theExpectDomainMatch RegionMatched RegionValueLRRNT40 . . . 6717/35(49%)0.001220/35(57%)LRR211 . . . 23410/25(40%)0.06321/25(84%)LRR283 . . . 30611/25(44%)0.1620/25(80%)LRR307 . . . 3309/25(36%)0.001623/25(92%)LRR382 . . . 4059/25(36%)0.1818/25(72%)LRRCT415 . . . 46522/54(41%)1.6e−1342/54(78%)ig484 . . . 55413/72(18%) 2e−0550/72(69%)ig588 . . . 64913/65(20%)0.02543/65(66%)ig682 . . . 74019/62(31%)1.4e−0845/62(73%)


Example 52

The NOV52 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 52A.

TABLE 52ANOV52 Sequence AnalysisNOV52a, CG59584-03SEQ ID NO:10914994 bpDNA SequenceORF Start: ATG at 36ORF Stop: TGA at 4815CCTGGACCTAATACCCCTTCTTCCTAAAGCACAAAATGATTAAATTGCTTAATCCAGACAACTCTCCAATCCCAAATGAAGTTGTTCAGTTGCATCTGAAGGACAAAATCGTGGGAAACTACACCACAGATGTAAATGGCATCGCTCAGTTTTTCTTGGACACATATACGTTTACATACCCAAATATCACTTTGAAAGCAGCCTACAAGGCCAATGAAAATTGCCAGGCTCATGGCTGGGTGTTGCCTCAATACCCTCAGCCCGAGTACTTTGCATATCGATTTTACTCCAAGATGAATAGCTTCCTAAAGATTGTCCAAGAGATGGAAGAACTGAGATGCAACCAGCAGAAGAGGGTGCTAGTGCACTGCATTCTCAATATGGAAGACTTTGAAGACAAAACCTACACAGCAGACTTCAATTATTTGGTGATTTCAAAAGGTGTAATCATTCTTCATGGGCAACAGAAAATTGAGATCAACGAAAATGGGAGGAAGGGCATATTTTCCATTTCTATAGACATTAACCCTGAATTAGCGCCCTCAGTAGATATGCTTGTCTATAGCTTGCATCCTGGAGGAGAAATGGTCACTGATAGCACCCAATTCCGAATTGAGAAATGCTTCGAAAATCAGGTCAACTTAAATTTTTCTAAAGAAAAAAGTTTACCAGGATCCAATATTGATCTTCAAGTCTCGGCTGCTTCAAACTCTCTTTGTGCTCTTTGGGCTGTAGACCAGAGTGTATTGCTACTAAGGAATTATGGTCAGCTGTCAGCACAAACTGTGTATAGTCAGCTATATTCCAGGGAACTACATGGCTATTACTTCAGAGGACTTAACTTAGAAGATGGCCTTAAAGTGCCGTGTCTTGAAGATGAACATATCCTTTACAATGGAATTTATTACACACCTGCATGGGCTGACTTTGGAAAAGATGGCTATGACCTTGTGAAGGATCCTCAAAACAATCGGATTTTTCAAAGGCAAAATGTGACTTCTTTCCGAAATATTACCCAACTCTCGTTCCAACTGATTTCAGAACCAATGTTTGGAGATTACTGGATTGTTGTGAAAAGAAACTCAAGGGAGACAGTGACACACCAATTTGCTGTTAAAAGATATGTGCTGCCCAAGTTTGAAGTTACAGTCAATGCACCACAAACAGTAACTATTTCAGATGATGAATTCCAAGTGGATGTATGTGCTAAGTACAACTTTGGCCAACCTGTGCAAGGGGAAACCCAAATCCGGGTGTGCAGAGAGTATTTTTCTTCAAGCAATTGTGAGAAAAATGAAAATGAAATATGTGAGCAATTTATTGCACAGTTGGAAAATGGTTGTGTTTCTCAAATTGTAAATACAAAAGTCTTCCAACTCTACCGTTCGGGATTGTTCATGACATTTCATGTCGCTGTAATTGTTACAGAATCTGGGACAGTTATGCAGATCAGCGAGAAGACCTCAGTTTTTATCACTCAATTGCTTGGAACTGTAAACTTTGAGAACATGGATACATTCTATAGAAGAGGGATTTCTTATTTTGGAACTCTTAAATTTTCGGATCCCAATAATGTACCTATGGTGAACAAGTTGTTGCAACTGGAGCTCAATGATGAATTTATAGGAAATTACACTACGGATGAGAATGGCGAAGCTCAATTTTCCATTGACACTTCAGACATATTTGATCCAGAGTTCAACCTAAAAGCCACATATGTTCGACCTGAGAGCTGCTATCTTCCCAGCTGGTTGACGCCTCAGTACTTGGATGCTCACTTCTTAGTCTCACGCTTTTACTCCCGAACCAACAGCTTCCTGAAGATTGTTCCAGAACCAAAGCAGCTTGAATGTAATCAACAGAAGGTTGTTACTGTGCATTACTCCCTAAACAGTGAAGCATATGAGGATGATTCCAATGTAAAGTTCTTCTATTTGATGATGGTAAAAGGAGCTATCTTACTCAGTGGACAAAAGGAAATCAGAAACAAAGCCTGGAATGGAAACTTCTCGTTCCCAATCAGCATCAGTGCTGATCTGGCTCCTGCAGCCGTCCTGTTTGTCTATACCCTTCACCCCAGTGGGGAAATTGTGGCTGACAGTGTCAGATTCCAGGTTGACAAGTGCTTTAAACACAAGGTTAACATAAAGTTCTCTAACGAGCAGGGCTTACCTGGTTCCAATGCTAGTCTCTGTCTTCAAGCGGCGCCTGTCTTATTCTGTGCCCTCAGGGCTGTGGATAGGAATGTCCTTCTACTGAAATCTGAACAACAGCTGTCAGCTGAAAGTGTGTATAACATGGTTCCAAGTATAGAGCCGTATGGTTATTTCTACCATGGCCTCAATCTTGATGATGGCAAGGAAGACCCTTGCATTCCTCAGAGGGATATGTTCTACAATGGTTTATATTACACACCTGTAAGCAACTATGGGGATGGAGATATCTATAATATTGTCAGGAACATGGGTCTAAAAGTCTTTACCAATCTCCATTACCGAAAACCAGAAAAAATTATGGTCCAATGCGTAGTGTTCCGTCTAGAATTGCATGTAGCTAGTGGAATCAGAGGGGAGAATGCTGACTATGTAGAACAGGCTATAATTCAAACAGTAAGAACAAACTTCCCAGAGACATGGATGTGGGACCTCGTCAGTGTCGATTCCTCAGGCTCTGCCAATCTTTCGTTCCTCATTCCTGATACGATAACCCAATGGGAGGCAAGTGGCTTTTGTGTGAATGGTGACGTTGGATTTGGCATTTCCTCTACAACCACTCTAGAAGTCTCCCAACCTTTCTTTATTGAGATTGCCTCACCCTTTTCGGTTGTTCAAAATGAACAATTTGATTTGATTGTCAATGTCTTCAGCTACCGGAATACATGTGTAGAGATTTCTGTTCAAGTGGAGGAGTCTCAGAATTATGAAGCAAATATTCATACCTTGAAAATCAATGGCAGTGAGGTTATTCAAGCTGGAGGGAGGAAAACAAACGTCTGGACTATTATACCTAAGAAATTGGGTAAAGTGAATATCACTGTAGTTGCTGAGTCCAAACAAAGCATTAGTAGAGCCTGAAGGTATTGAAAAGGAAAGGACCCAGAGTTTCCTTATCTGTACAGAAGGTGCCAACTGTTGTGGGGGATATTCTAGGACTTGCCTTGCAGAATCTGGTTGTTCTCCAAATGCCCTATGGAAGTGGAGAGCAGAATGCTGCCCTACTAGCATCTGATACTTATGTTCTGGACTATCTGAAATCTACTGAGCAACTGACAGAGGAAGTTCAATCTAAGGCTTTCTTTCTCTTATCTAATGGTTATCAAAGGCAATTATCTTTCAAAAACTCTGATGGTTCCTATAGTGTGTTTTGGCAGCAGAGTCAGAAAGGAAGCATATGGCTCAGTGCTCTTACTTTTAAGACATTGGAGAGAATGAAAAAATATGTATTCATTGATGAAAATGTTCAAAAACAGACCTTAATCTGGCTTTCAAGCCAACAGAAAACAAGCGGCTGCTTTAAGAATGATGGCCAGCTTTTCAACCACGCCTGGGAGGGTGGAGATGAAGAGGACATTTCACTCACTGCGTATGTTGTTGGGATGTTCTTTGAAGCTGGGCTCAATTTCACTTTTCCTGCTCTACGAAACGCACTCTTTTGCCTTGAAGCGGCATTGGACAGTGGTGTCACTAATGGCTATAATCATGCAATTCTAGCTTATGCTTTTGCCTTAGCTGGAAAAGAGAAGCAAGTGGAATCTTTACTCCAAACCCTGGATCAATCTGCCCCAAAACTAAATAATGTCATCTACTGGTGAGAAGAGTTGCTACGTGCTGTTGGCTGTCATTTCCCGGAAAATTCCTGACCTCACCTATGCTAGTAAGATTGTGCAGTGGCTTGCCCAACGGATGAATTCCCATGGAGGCTTTTCTTCCAACCAGACACCTGATGATACTCTGTTCAAATTATATACGGGCCAAAAAGAAAGCTTTCGCTCTAGTTCTGTGGGCTATACACTGGGAAAAGCAAATGAAAAGAAGGAAAACAGGAGAAATGGGGGTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAACCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGAGAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCACAAGAATGACCATGTTCTTTTCTACTTGGAAAATGTAGGTTTTGGTCGAGCAGACAGTTTCCCTTTTTCTGTTGAGCAGAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTATTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGTGAGACAAAGCAATTACTAGAAGAGTTGGAGAAGCATTTCTTGTAACAAACTGATTCTTCTGTATCAAACCTGGAAAAAAATCATGAACCATCTGACATCGTGAACAGTCTGCAGTGGGCTATGGTTTCTTGTCAAGTCTTATTTCCTTATCATCCCATTAAATGTTGTCATTTTGCAAANOV52a CG59584-03SEQ ID NO:10921593 aaMW at 180167.3 kDProteinMIKLLNPDNSPIPNEVVQLHLKDKIVGNYTTDVNGIAQFFLDTYTFTYPNITLKAAYKANENCQAHGWVLPQYPQPEYFAYRFYSKMNSFLKIVQEMEELRCNQQKRVLVHCILNMEDFEDKTYTADFNYLVISKGVIILHGQQKIEINENGRKGIFSISIDINPELAPSVDMLVYSLHPGGEMVTDSTQFRIEKCFENQVNLNFSKEKSLPGSNIDLQVSAASNSLCALWAVDQSVLLLRNYGQLSAQTVYSQLYSRELHGYYFRGLNLEDGLKVPCLEDEHILYNGIYYTPAWADFGKDGYDLVKDPQNNRIFQRQNVTSFRNITQLSFQLISEPMFGDYWIVVKRNSRETVTHQFAVKRYVLPKFEVTVNAPQTVTISDDEFQVDVCAKYNFGQPVQGETQIRVCREYFSSSNCEKNENEICEQFIAQLENGCVSQIVNTKVFQLYRSGLFMTFHVAVIVTESGTVMQISEKTSVFITQLLGTVNFENMDTFYRRGISYFGTLKFSDPNNVPMVNKLLQLELNDEFIGNYTTDENGEAQFSIDTSDIFDPEFNLKATYVRPESCYLPSWLTPQYLDAHFLVSRFYSRTNSFLKIVPEPKQLECNQQKVVTVHYSLNSEAYEDDSNVKFFYLMMVKGAILLSGQKEIRNKAWNGNFSFPISISADLAPAAVLFVYTLHPSGEIVADSVRFQVDKCFKHKVNIKFSNEQGLPGSNASLCLQAAPVLFCALRAVDRNVLLLKSEQQLSAESVYNMVPSIEPYGYFYHGLNLDDGKEDPCIPQRDMFYNGLYYTPVSNYGDGDIYNIVPNMGLKVFTNLHYRKPEKIMVQCVVFRLELHVASGIRGENADYVEQAIIQTVRTNFPETWMWDLVSVDSSGSANLSFLIPDTITQWEASGFCVNGDVGFGISSTTTLEVSQPFFIEIASPFSVVQNEQFDLIVNVFSYRNTCVEISVQVEESQNYEANIHTLKINGSEVIQAGGRKTNVWTIIPKKLGKVNITVVAESKQSSACPNEGMEQQKLNWKDTVVQSFLVEPEGIEKERTQSFLICTEGAKASKQGVLDLPNDVVEGSARGFFTVVGDILGLALQNLVVLQMPYGSGEQNAALLASDTYVLDYLKSTEQLTEEVQSKAFFLLSNGYQRQLSFKNSDGSYSVFWQQSQKGSIWLSALTFKTLERMKKYVFIDENVQKQTLIWLSSQQKTSGCFKNDGQLFNHAWEGGDEEDISLTAYVVGMFFEAGLNFTFPALRNALFCLEAALDSGVTNGYNHAILAYAFALAGKEKQVESLLQTLDQSAPKLNNVIYWERERKPKTEEFPSFIPWAPSAQTEKSCYVLLAVISRKIPDLTYASKIVQWLAQRMNSHGGFSSNQTPDDTLFKLYTGQKESFRSSSVGYTLGKANEKKENRRNGGEGSSEIFQVNGHNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENVGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSENOV52b, CG59584-02SEQ ID NO:10934501 bpDNA SequenceORF Start: at 1ORF Stop: TGA at 4309TCCATTTCTATAGACATTAACCCTGAATTAGCGCCCTCAGTAGATATGCTTGTCTATAGCTTGCATCCTGGAGGAGAAATGGTCACTGATAGCACCCAATTCCGAATTGAGAAATGCTTCGAAAATCAGGTCAACTTAAATTTTTCTAAAGAAAAAAGTTTACCAGGATCCAATATTGATCTTCAAGTCTCGGCTGCTTCAAACTCTCTTTGTGCTCTTTGGGCTGTAGACCAGAGTGTATTGCTACTAAGGAATTATGGTCAGCTGTCAGCACAAACTGTGTATAGTCAGCTATATTCCAGGGAACTACATGGCTATTACTTCAGAGGACTTAACTTAGAAGATGGCCTTAAAGTGCCGTGTCTTGAAGATGAACATATCCTTTACAATGGAATTTATTACACACCTGCATGGGCTGACTTTGGAAAAGATGGCTATGACCTTGTGAAGGATCCTCAAAACAATCGGATTTTTCAAAGGCAAAATGTGACTTCTTTCCGAAATATTACCCAACTCTCGTTCCAACTGATTTCAGAACCAATGTTTGGAGATTACTGGATTGTTGTGAAAAGAAACTCAAGGGAGACAGTGACACACCAATTTGCTGTTAAAAGATATGTGCTGCCCAAGTTTGAAGTTACAGTCAATGCACCACAAACAGTAACTATTTCAGATGATGAATTCCAAGTGGATGTATGTGCTAAGTACAACTTTGGCCAACCTGTGCAAGGGGAAACCCAAATCCGGGTGTGCAGAGAGTATTTTTCTTCAAGCAATTGTGAGAAAAATGAAAATGAAATATGTGAGCAATTTATTGCACAGTTGGAAAATGGTTGTGTTTCTCAAATTGTAAATACAAAAGTCTTCCAACTCTACCGTTCGGGATTGTTCATGACATTTCATGTCGCTGTAATTGTTACAGAATCTGGGACAGTTATGCAGATCAGCGAGAAGACCTCAGTTTTTATCACTCAATTGCTTGGAACTGTAAACTTTGAGAACATGGATACATTCTATAGAAGAGGGATTTCTTATTTTGGAACTCTTAAATTTTCGGATCCCAATAATGTACCTATGGTGAACAAGTTGTTGCAACTGGAGCTCAATGATGAATTTATAGGAAATTACACTACGGATGAGAATGGCGAAGCTCAATTTTCCATTGACACTTCAGACATATTTGATCCAGAGTTCAACCTAAAAGCCACATATGTTCGACCTGAGAGCTGCTATCTTCCCAGCTGGTTGACGCCTCAGTACTTGGATGCTCACTTCTTAGTCTCACGCTTTTACTCCCGAACCAACAGCTTCCTGAAGATTGTTCCAGAACCAAAGCAGCTTGAATGTAATCAACAGAAGGTTGATGATGGTAAAAGGAGCTATCTTACTCAGTGGACAAAAGGAAATCAGAAACAAAGCCTGGAATGGTTCACCCCAGTGGGGAAATTGTGGCTGACAGTGTCAGATTCCAGGTTGACAAGTGCTTTAAACACAAGGTTAACATAAAGTTCTCTAACGAGCAGGGCTTACCTGGTTCCAATGCTAGTCTCTGTCTTCAAGCGGCGCCTGTCTTATTCTGTGCCCTCAGGGCTGTGGATAGGAATGTCCTTCTACTGAAATCTGAACAACAGCTGTCAGCTGAAAGTGTGTATAACATGGTTCCAAGTATAGAGCCGTATGGTTATTTCTACCATGGCCTCAATCTTGATGATGGCAAGGAAGACCCTTGCATTCCTCAGAGGGATATGTTCTACAATGGTTTATATTACACACCTGTAAGCAACTATGGGGATGGAGATATCTATAATATTGTCAGGAACATGGGTCTAAAAGTCTTTACCAATCTCCATTACCGAAAACCAGAAAAAATTATGGTCCAATGCGTAGTGTTCCGTCTAGAATTGCATGTAGCTAGTGGAATCAGAGGGGAGAATGCTGACTATGTAGAACAGGCTATAATTCAAACAGTAAGAACAAACTTCCCAGAGACATGGATGTGGGACCTCGTCAGTGTCGATTCCTCAGGCTCTGCCAATCTTTCGTTCCTCATTCCTGATACGATAACCCAATGGGAGGCAAGTGGCTTTTGTGTGAATGGTGACGTTGGATTTGGCATTTCCTCTACAACCACTCTAGAAGTCTCCCAACCTTTCTTTATTGAGATTGCCTCACCCTTTTCGGTTGTTCAAAATGAACAATTTGATTTGATTGTCAATGTCTTCAGCTACCGGAATACATGTGTAGAGATTTCTGTTCAAGTGGAGGAGTCTCAGAATTATGAAGCAAATATTCATACCTTGAAAATCAATGGCAGTGAGGTTATTCAAGCTGGAGGGAGGAAAACAAACGTCTGGACTATTATACCTAAGAAATTGGGTAAAGTGAATATCACTGTAGTTGCTGAGTCCAAACAAAGCAGTGCTTGCCCAAATGAAGGAATGGAGCAGCAAAAGCTAAACTGGAAAGACACTGTGGTCCAAAGCTTCTTAGTAGAGCCTGAAGGTATTGAAAAGGAAAGGACCCAGAGTTTCCTTATCTGTACAGAAGGTGCCAAAGCCTCCAAGCAGGGAGTTTTGGACTTGCCAAACGATGTAGTAGAAGGGTCAGCCAGAGGCTTTTTCACTGTTGTGGGGGATATTCTAGGACTTGCCTTGCAGAATCTGGTTGTTCTCCAAATGCCCTATGGAAGTGGAGAGCAGAATGCTGCCCTACTAGCATCTGATACTTATGTTCTGGACTATCTGAAATCTACTGAGCAACTGACAGAGGAAGTTCAATCTAAGGCTTTCTTTCTCTTATCTAATGGTTATCAAAGGCAATTATCTTTCAAAAACTCTGATGGTTCCTATAGTGTGTTTTGGCAGCAGAGTCAGAAAGGAAGCATATGGCTCAGTGCTCTTACTTTTAAGACATTGGAGAGAATGAAAAAATATGTATTCATTGATGAAAATGTTCAAAAACAGACCTTAATCTGGCTTTCAAGCCAACAGAAAACAAGCGGCTGCTTTAAGAATGATGGCCAGCTTTTCAACCACGCCTGGGAGGGTGGAGATGAAGAGGACATTTCACTCACTGCGTATGTTGTTGGGATGTTCTTTGAAGCTGGGCTCAATTTCACTTTTCCTGCTCTACGAAACGCACTCTTTTGCCTTGAAGCGGCATTGGACAGTGGTGTCACTAATGGCTATAATCATGCAATTCTAGCTTATGCTTTTGCCTTAGCTGGAAAAGAGAAGCAAGTGGAATCTTTACTCCAAACCCTGGATCAATCTGCCCCAAAACTAAATAATGTCATCTACTGGGAAAGAGAAAGGAAACCCAAGACAGAAGAATTTCCATCCTTTATTCCCTGGGCACCTTCTGCTCAGACTGAGAAGAGTTGCTACGTGCTGTTGGCTGTCATTTCCCGGAAAATTCCTGACCTCACCTATGCTAGTAAGATTGTGCAGTGGCTTGCCCAACGGATGAATTCCCATGGAGGCTTTTCTTCCAACCAGACACCTGATGATACTCTGTTCAAATTATATACGGGCCAAAAAGAAAGCTTTCGCTCTAGTTCTGTGGGCTATACACTGGGAAAAGCAAATGAAAAGAAGGAAAACAGGAGAAATGGGGGTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAACCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGAGAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCATTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATGTAGGTTTTGGTCGAGCAGACAGTTTCCCTTTTTCTGTTGAGCAGAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTATTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGTGAGACAAAGCAATTACTAGAAGAGTTGGAGAAGCATTTCTTGTAACAAACTGATTCTTCTGTATCAAACCTGGAAAAAAATCATGAACCATCTGACATCGTGAACAGTCTGCAGTGGGCTATGGTTTCTTGTCAAGTCTTATTTCCTTATCATCCCATTAAATGTTGTCATTTTGCAAAAAAAAAAAAAAAANOV52b, CG59584-02SEQ ID NO:10941436 aaMW at 161836.4 kDProtein SequenceSISIDINPELAPSVDMLVYSLHPGGEMVTDSTQFRIEKCFENQVNLNFSKEKSLPGSNIDLQVSAASNSLCALWAVDQSVLLLRNYGQLSAQTVYSQLYSRELHGYYFRGLNLEDGLKVPCLEDEHILYNGIYYTPAWADFGKDGYDLVKDPQNNRIFQRQNVTSFRNITQLSFQLISEPMFGDYWIVVKRNSRETVTHQFAVKRYVLPKFEVTVNAPQTVTISDDEFQVDVCAKYNFGQPVQGETQIRVCREYFSSSNCEKNENEICEQFIAQLENGCVSQIVNTKVFQLYRSGLFMTFHVAVIVTESGTVMQISEKTSVFITQLLGTVNFENMDTFYRRGISYFGTLKFSDPNNVPMVNKLLQLELNDEFIGNYTTDENGEAQFSIDTSDIFDPEFNLKATYVRPESCYLPSWLTPQYLDAHFLVSRFYSRTNSFLKIVPEPKQLECNQQKVVTVHYSLNSEAYEDDSNVKFFYLMMVKGAILLSGQKEIRNKAWNGNFSFPISISADLAPAAVLFVYTLHPSGEIVADSVRFQVDKCFKHKVNIKFSNEQGLPGSNASLCLQAAPVLFCALRAVDRNVLLLKSEQQLSAESVYNMVPSIEPYGYFYHGLNLDDGKEDPCIPQRDMFYNGLYYTPVSNYGDGDIYNIVRNMGLKVFTNLHYRKPEKIMVQCVVFRLELHVASGIRGENADYVEQAIIQTVRTNFPETWMWDLVSVDSSGSANLSFLIPDTITQWEASGFCVNGDVGFGISSTTTLEVSQPFFIEIASPFSVVQNEQFDLIVNVFSYRNTCVEISVQVEESQNYEANIHTLKINGSEVIQAGGRKTNVWTIIPKKLGKVNITVVAESKQSSACPNEGMEQQKLNWKDTVVQSFLVEPEGIEKERTQSFLICTEGAKASKQGVLDLPNDVVEGSARGFFTVVGDILGLALQNLVVLQMPYGSGEQNAALLASDTYVLDYLKSTEQLTEEVQSKAFFLLSNGYQRQLSFKNSDGSYSVFWQQSQKGSIWLSALTFKTLERMKKYVFIDENVQKQTLIWLSSQQKTSGCFKNDGQLFNHAWEGGDEEDISLTAYVVGMFFEAGLNFTFPALRNALFCLEAALDSGVTNGYNHAILAYAFALAGKEKQVESLLQTLDQSAPKLNNVIYWERERKPKTEEFPSFIPWAPSAQTEKSCYVLLAVISRKIPDLTYASKIVQWLAQRMMSHGGFSSNQTPDDTLFKLYTGQKESFRSSSVGYTLGKANEKKENRRNGGEGSSEIFQVNGHNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKNLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENVGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSENOV52c 248210405SEQ ID NO:1095571 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATGTGAAGGATCCAGTGAGATTTTCCACCTTAACGGTCATAATCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGAGAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCATTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATGGTTTTGGTCGAGCAGACAGTTTCCCTTTTTCTGTTGAGCAGAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTACTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGCTCGAGGGCNOV52c, 248210405SEQ ID NO:1096190 aaMW at 21231.7 kDProtein SequenceTRSEGSSEIFQVNGHNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENGFGRASFPFSVEQSNLVFNIQPAPDYYEKEEYALAFYNIDSSSVSELEGNOV52d, 248210436SEQ ID NO:1097571 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAACCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGACAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCATTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATGGTTTTGGTCGAGCAGACAGTTTCCCTTTTTCTGTTGAGCAGAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTACTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGCTCGAGGGCNOV52d, 248210436SEQ ID NO:1098190 aaMW at 21230.7 kDProtein SequenceTRSEGSSEIFQVNGHNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEGNOV52e 249357737SEQ ID NO:1099571 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAACCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGACAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACGATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCATTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATGGTTTTGGTCGAGCAGACAGTTTCCCTTTTTCTGTTGAGCAGAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTACTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGCTCGAGGGCNOV52e, 249357737SEQ ID NO:1100190 aaMW at 21231.7 kDProtein SequenceTRSEGSSEIFQVNGHNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYDVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENGFGRADSFPFSVEQSNLVFNIQPAPDYYEKEEYALAFYNIDSSSVSELEGNOV52f, 248210430SEQ ID NO:1101529 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAACCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGACAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCATTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTACTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGCTCGAGGGCNOV52f, 248210430SEQ ID NO:1102176 aaMW at 19705.1 kDProtein SequenceTRSEGSSEIFQVNGHNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEGNOV52g, 249357755SEQ ID NO:1103547 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAATCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGACAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCACTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTACTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGCTCGAGGGCAAGGGCGAATCCAGCACANOV52g, 249357755SEQ ID NO:1104182 aaMW at 20282.6 kDProtein SequenceTRSEGSSEIFQVNGHNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIPNKSSMVVIDVKMLSGFTPTMSSTEELENKGQVMKTEVKNDHVLFYLENSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEGKGESSTNOV52h, CG59584-01SEQ ID NO:11054426 bpDNA SequenceORF Start: ATG at 1ORF Stop: TGA at 4234ATGTACTGTTTTTTGCGATCTGCCGTTTCGTTCTTCTGCCTCAGCCTCCCCAGGTGCTGGGGTTATAGGTGTGAGCCACTGTGCCTGGCTATTCTTTTATTACAGTATGTTCTGCTGATTCCTTCTGTTCTACAAGAAGGCTCTTTGGATAAAGCTTGTGCCCAGCTTTTTAATCTCACTGAATCTGTTGTTTTGACGGTCTCCCTCAACTATGGTGAGGTCCAGACCAAAATATTTGAAGAAAATGTTACTGGAGAAAATTTCTTCAAATGCATCAGCTTTGAGGTTCCTCAGGCCAGATCTGACCCACTGGCATTTATTACATTTTCTGCTAAAGGAGCCACTCTCAACCTGGAAGAGAGGAGATCTGTGGCAATCAGATCCAGAGAGAATGTGGTCTTTGTACAGACTGATAAACCCACCTACAAGCCTGGACAGTATAATAAAAAGCCGATCAGTCACATAATGCCAGTGATAGCAGTCACTGAACAGGATCCAGAAGGCAATCGAATACAACAGTGGGTGAATGAGGAGTCTGTGGGAGGGATTCTACAACTCTCCTTCCAGTTAATCTCAGAGCCCATCCTCGGATGGTATGAAATCACCGTGGAGATGCTCAATGAGAAGAAAACATATCACTCCTTCTCTGTGGAAGAATATGTGTTACCCAAATTTCAAATGACTGTGGATGCACCAGAAAATATCTTAGTTGTGGACTCTGAATTCAAAGTGAATGTCTGTGCCTTGTATACCTATGGTGAACCTGTGGACGGGAAGGTCCAACTTAGTGTGTGCAGAGAATCTACGGCTTATCATTCATGTGCTCATCTTATCAGTTCACTCTGTAAAAATTTTACCTTGGGGAAAGATGGCTGTGTCTCCAAGTTTATTAACACAGATGCTTTTGAGTTAAATCGGGAAGGATACTGGAGTTTCCTCAAAGTGCATGCTCTTGTTACAGAGCTTACAGGCTCCAAGTACGTATACATAGACTCATCAGTGGTGAAGATTAGTTTTGAGATGTAAATGGCATCGCTCAGTTTTTCTTGGACACATATACGTTTACATACCCAAATATCACTTTGAAAGCAGCCTACAAGGCCAATGAAAATTGCCAGGCTCATGGCTGGGTGTTGCCTCAATACCCTCAGCCCGAGTACTTTGCATATCGATTTTACTCCAAGATGAATAGCTTCCTAAAGATTGTCCAAGAGATGGAAGAACTGAGATGCAACCAGCAGAAGAGGGTGCTAGTGCACTGCATTCTCAATATGGAAGACTTTGAAGACAAAACCTACACAGCAGACTTCAATTATTTGGTGATTTCAAAAGGTGTAATCATTCTTCATGGGCAACAGAAAATTGAGATCAACGAAAATGGGAGGAAGGGCATATTTTCCATTTCTATAGACATTAACCCTGAATTAGCGCCCTCAGTACATATGCTTGTCTATAGCTTGCATCCTGGAGGAGAAATGGTCACTGATAGCACCCAATTCCAATTGAGAAATGTTAACATAAAGTTCTCTAACGAGCAGGGCTTACCTGGTTCCAATGCTAGTCTCTGTCTTCAAGCGGCGCCTGTCTTATTCTGTGCCCTCAGGGCTGTGGATAGGAATGTCCTTCTACTGAAATCTGAACAACAGCTGTCAGCTGAAAGTGTGTATAACATGGTTCCAAGTATAGAGCCGTATGGTTATTTCTACCATGGCCTCAATCTTGATGATGGCAAGGAAGACCCTTGCATTCCTCAGAGGGATATGTTCTACAATGGTTTATATTACACACCTGTAAGCAACTATGGGGATGGAGATATCTATAATATTGTCAGGAACATGGGTCTAAAAGTCTTTACCAATCTCCATTACCGAAAACCAGAAGTATGTGTGATGGAGAGAAGGCTGCCACTCCCTAAGCCGCTTTATCTGGAAACAGAAAATTATGGTCCAATGCGTAGTGTTCCGTCTAGAATTGCATCTAGTGGAATCAGAGGGGAGAATGCTGACTATGTAGAACAGGCTATAATTCAAACAGTAAGAACAAACTTCCCAGAGACATGGATGTGGGACCTCGTCAGTGTCGATTCCTCAGGCTCTGCCAATCTTTCGTTCCTCATTCCTGATACGATAACCCAATGGGAGGCAAGTGGCTTTTGTGTGAATGGTGACGTTGGATTTGGCATTTCCTCTACAACCACTCTAGAAGTCTCCCAACCTTTCTTTATTGAGATTGCCTCACCCTTTTCGGTTGTTCAAAATGAACAATTTGATTTGATTGTCAATGTCTTCAGCTACCGGAATACATGTGTAGAGATTTCTGTTCAAGTGGAGGAGTCTCAGAATTATGAAGCAAATATTCATACCTTGAAAATCAATGGCAGTGAGGTTATTCAAGCTGGAGGGAGGAAAACAAACGTCTGGACTATTATACCTAAGAAATTGGGCAAAGTGAATATCACTGTAGTTGCTGAGTCCAAACAAAGCAGTGCTTGCCCAAATGAAGGAATGGAGCAGCAAAAGCTAAACTGGAAAGACACTGTGGTCCAAAGCTTCTTAGTAGAGCCTGAAGGTATTGAAAAGGAAAGGACCCAGAGTTTCCTTATCTGTACAGAAGGTGCCAAAGCCTCCAAGCAGGGAGTTTTGGACTTGCCAAACGATGTAGTAGAAGGGTCAGCCAGAGGCTTTTTCACTGTTGTGGGGGATATTCTAGGACTTGCCTTGCAGAATCTGGTTGTTCTCCAAATGCCCTATGGAAGTGGAGAGCAGAATGCTGCCCTACTAGCATCTGATACTTATGTTCTGGACTATCTGAAATCTACTGAGCAACTGACAGAGGAAGTTCAATCTAAGGCTTTCTTTCTCTTATCTAATGGTTATCAAAGGCAATTATCTTTCAAAAACTCTGATGGTTCCTATAGTGTGTTTTGGCAGCAGAGTCAGAAAGGAAGCATATGGCTCAGTGCTCTTACTTTTAAGACATTGGAGAGAATGAAAAAATATGTATTCATTGATGAAAATGTTCAAAAACAGACCTTAATCTGGCTTTCAAGCCAACAGAAAACAAGCGGCTGCTTTAAGAATGATGGCCAGCTTTTCAACCACGCCTGGCAGGGTGGAGATGAAGAGGACATTTCACTCACTGCGTATGTTGTTGGGATGTTCTTTGAAGCTGGGGCGGCATTGGACAGTGGTGTCACTAATGGCTATAATCATGCAATTCTAGCTTATGCTTTTGCCTTAGCTGGAAAAGAGAAGCAAGTGGAATCTTTACTCCAAACCCTGGATCAATCTGCCCCAAAACTAAATAATGTCATCTACTGGGAAAGAGAAAGGAAACCCAAGACAGAAGAATTTCCATCCTTTATTCCCTGGGCACCTTCTGCTCAGACTGAGAAGAGTTGCTACGTGCTGTTGGCTGTCATTTCCCGGAAAATTCCTGACCTCACCTATGCTAGTAAGATTGTGCAGTGGCTTGCCCAACGGATGAATTCCCATGGAGGCTTTTCTTCCAACCAGGATCAAAACACTGTCACCTTTAGCAGTGAAGGATCCAGTGAGATTTTCCAGGTTAACGGTCATAACCGCCTACTGGTCCAACGTTCAGAAGTAACACAGGCACCTGGAGAATACACAGTAGATGTGGAAGGACACGGTTGTACATTTATCCAGGCCACCCTTAAGTACAATGTTCTCCTACCTAAGAAGGCATCTGGATTTTCTCTTTCCTTGGAAATAGTAAAGAACTACTCTTCGACTGCTTTTGACCTCACAGTGACCCTCAAATACACTGGAATTCGCAATAAATCCAGTATGGTGGTTATAGATGTAAAAATGCTATCAGGATTTACTCCAACCATGTCATCCATTGAAGAGCTTGAAAACAAGGGCCAAGTGATGAAGACTGAAGTCAAGAATGACCATGTTCTTTTCTACTTGGAAAATGTAGGTTTTGGTCGAGCAGACAGTTTCCCTTTTTCTGTTGAGCAGAGCAACCTTGTGTTCAACATTCAGCCAGCCCCAGCCATGGTCTACGATTATTATGAAAAAGAAGAATATGCCCTAGCTTTTTACAACATCGACAGTAGTTCAGTTTCCGAGTGAGACAAAGCAATTACTAGAAGAGTTGGAGAAGCATTTCTTGTAACAAACTGATTCTTCTGTATCAAACCTGGAAAAAAATCATGAACCATCTGACATCGTGAACAGTCTGCAGTGGGCTATGGTTTCTTGTCAAGTCTTATTTCCTTATCATCCCATTAAATGTTGTCATTTTGCAAAAAAAAAAAAAAAANOV52h, CG59584-01SEQ ID NO:11061411 aaMW at 158867.0 kDProtein SequenceMYCFLRSAVSFFCLSLPRCWGYRCEPLCLAILLLQYVLLIPSVLQEGSLDKACAQLFNLTESVVLTVSLNYGEVQTKIFEENVTGENFFKCISFEVPQARSDPLAFITFSAKGATLNLEERRSVAIRSRENVVFVQTDKPTYKPGQYNKKPISHIMPVIAVTEQDPEGNRIQQWVNEESVGGILQLSFQLISEPTLGWYEITVEMLNEKKTYHSFSVEEYVLPKFQMTVDAPENILVVDSEFKVNVCALYTYGEPVDGKVQLSVCRESTAYHSCAHLISSLCKNFTLGKDGCVSKFINTDAFELNREGYWSFLKVHALVTELTGSKYVYIDSSVVKISFENMDMSYKQGLPYFGQIKLLNPDNSPIPNEVVQLHLKDKIVGNYTTDVNGIAQFFLDTYTFTYPNITLKAAYKANENCQAHGWVLPQYPQPEYFAYRFYSKMNSFLKIVQENEELRCNQQKRVLVHCILNMEDFEDKTYTADFNYLVISKGVIILHGQQKIEINENGRKGIFSISIDINPELAPSVHMLVYSLHPGGEMVTDSTQFQLRNVNIKFSNEQGLPGSNASLCLQAAPVLFCALRAVDRNVLLLKSEQQLSAESVYNMVPSIEPYGYFYHGLNLDDGKEDPCIPQRDMFYNGLYYTPVSNYGDGDIYNIVRNMGLKVFTNLHYRKPEVCVMERRLPLPKPLYLETENYGPMRSVPSRIASSGIRGENADYVEQAIIQTVRTNFPETWMWDLVSVDSSGSANLSFLIPDTITQWEASGFCVNGDVGFGISSTTTLEVSQPFFIEIASPFSVVQNEQFDLIVNVFSYRNTCVEISVQVEESQNYEANIHTLKINGSEVIQAGGRKTNVWTIIPKKLGKVNITVVAESKQSSACPNEGMEQQKLNWKDTVVQSFLVEPEGIEKERTQSFLICTEGAKASKQGVLDLPNDVXTEGSARGFFTWGDILGLALQNLVVLQMPYGSGEQNAALLASDTYVLDYLKSTEQLTEEVQSKAFFLLSNGYQRQLSFKNSDGSYSVFWQQSQKGSIWLSALTFKTLERMKKYVFIDENVQKQTLIWLSSQQKTSGCFKNDGQLFNHAWQGGDEEDISLTAYVVGMFFEAGAALDSGVTNGYNHAILAYAFALAGKEKQVESLLQTLDQSAPKLNNVIYWERERKPKTEEFPSFIPWAPSAQTEKSCYVLLAVISRKIPDLTYASKIVQWLAQRMNSHGGFSSNQDQNTVTFSSEGSSEIFQVNGHNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTAFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENVGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSE


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 52B.

TABLE 52BComparison of the NOV52 protein sequences.NOV52aMIKLLNPDNSPIPNEVVQLHLKDKIVGNYTTDVNGIAQFFLDTYTFTYPNITLKAAYKAN(SEQ ID NO:1092)NOV52b------------------------------------------------------------(SEQ ID NO:1094)NOV52c------------------------------------------------------------(SEQ ID NO:1096)NOV52d------------------------------------------------------------(SEQ ID NO:1098)NOV52e------------------------------------------------------------(SEQ ID NO:1100)NOV52f------------------------------------------------------------(SEQ ID NO:1102)NOV52g------------------------------------------------------------(SEQ ID NO:1104)NOV52h------------------------------------------------------------(SEQ ID NO:1106)NOV52aENCQAHGWVLPQYPQPEYFAYRFYSKMNSFLKIVQEMEELRCNQQKRVLVHCILNMEDFENOV52b------------------------------------------------------------NOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52h--------------------------MYCFLRSAVSFFCLSLPR----------------NOV52aDKTYTADFNYLVISKGVIILHGQQKIEINENGRKGIFSISIDINPELAPSVDMLVYSLHPNOV52b-------------------------------------SISIDINPELAPSVDMLVYSLHPNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52h--------------------------------CWGYRCEPLCLAILLLQYVLLIPSVLQENOV52aGGEMVTDSTQFRIEKCFENQVNLNFSKEKSLPGSNIDLQVSAASNSLCALWAVDQSVLLLNOV52bGGEMVTDSTQFRIEKCFENQVNLNFSKEKSLPGSNIDLQVSAASNSLCALWAVDQSVLLLNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hGSLDKACAQLFNLTESVVLTVSLNYGEVQTK---IFEENVTGENFFKCISFEVPQARSDPNOV52aRNYGQLSAQTVYSQLYSRELHGYYFRGLNLEDGLKVPCLEDEHILYNGIYYTPAWADFGKNOV52bRNYGQLSAQTVYSQLYSRELHGYYFRGLNLEDGLKVPCLEDEHILYNGIYYTPAWADFGKNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hLAFITFSAKGATLNLEERRSVAIRSRENVVFVQTDKPTYKPGQYNKKPISHIMPVIAVT-NOV52aDGYDLVKDPQNNRIFQRQNVTSFRNITQLSFQLISEPMFGDYWIVVKRNSRETVTHQFAVNOV52bDGYDLVKDPQNNRIFQRQNVTSFRNITQLSFQLTSEPMFGDYWIVVKRNSRETVTHQFAVNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52h-----EQDPEGNRIQQWVNEESVGGILQLSFQLISEPILGWYEITVEMLNEKKTYHSFSVNOV52aKRYVLPKFEVTVNAPQTVTISDDEFQVDVCAKYNFGQPVQGETQIRVCREYFSSSNCEKNNOV52bKRYVLPKFEVTVNAPQTVTISDDEFQVDVCAKYNFGQPVQGETQIRVCREYFSSSNCEKNNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hEEYVLPKFQMTVDAPENILVVDSEFKVNVCALYTYGEPVDGKVQLSVCRESTAYHSCAHLNOV52aENEICEQFIAQLENGCVSQIVNTKVFQLYRSGLFMTFHVAVIVTESGTVMQISEKTSVFINOV52bENEICEQFIAQLENGCVSQIVNTKVFQLYRSGLFMTFHVAVIVTESGTVMQISEKTSVFINOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hISSLCKNFTLGKD-GCVSKFINTDAFELNREGYWSFLKVEALVTE------LTGSKYVYINOV52aTQLLGTVNFENMDTFYRRGISYFGTLKFSDPNNVPMVNKLLQLELNDEFIGNYTTDENGENOV52bTQLLGTVNFENMDTFYRRGISYFGTLKFSDPNNVPMVNKLLQLELNDEFIGNYTTDENGENOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hDSSVVKISFENMDMSYKQGLPYFGQIKLLNPDNSPIPNEVVQLHLKDKIVGNYTTDVNGINOV52aAQFSIDTSDIFDPEFNLKATYVRPESCYLPSWLTPQYLDAHFLVSRFYSRTNSFLKIVPENOV52bAQFSIDTSDIFDPEFNLKATYVRPESCYLPSWLTPQYLDAHFLVSRFYSRTNSFLKIVPENOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hAQFFLDTYTFTYPNITLKAAYKANENCQAHGWVLPQYPQPEYFAYRFYSKMNSFLKIVQENOV52aPKQLECNQQKVVTVHYSLNSEAYEDDS-NVKFFYLMMVKGAILLSGQK--EIRNKAWNGNNOV52bPKQLECNQQKVVTVHYSLNSEAYEDDS-NVKFFYLMMVKGAILLSGQK--EIRNKAWNGNNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hMEELRCNQQKRVLVHCILNMEDFEDKTYTADFNYLVISKGVIILHGQQKIEINENGRKGINOV52aFSFPISISADLAPAAVLFVYTLHPSGEIVADSVRFQVDKCFKHKVNIKFSNEQGLPGSNANOV52bFSFPISISADLAPAAVLFVYTLHPSGEIVADSVRFQVDKCFKHKVNIKFSNEQGLPGSNANOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hFSISIDINPELAPSVHMLVYSLHPGGEMVTDSTQFQLR-----NVNIKFSNEQGLPGSNANOV52aSLCLQAAPVLFCALRAVDRNVLLLKSEQQLSAESVYNMVPSIEPYGYFYHGLNLDDGKEDNOV52bSLCLQAAPVLFCALRAVDRNVLLLKSEQQLSAESVYNMVPSIEPYGYFYHGLNLDDGKEDNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hSLCLQAAPVLFCALRAVDRNVLLLKSEQQLSAESVYNMVPSIEPYGYFYHGLNLDDGKEDNOV52aPCIPQRDMFYNGLYYTPVSNYGDGDIYNIVPNMGLKVFTNLHYRKPEKIMVQCVVFR---NOV52bPCIPQRDMFYNGLYYTPVSNYGDGDIYNIVRNMGLKVFTNLHYRKPEKIMVQCVVFR---NOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hPCIPQRDMFYNGLYYTPVSNYGDGDIYNIVRNMGLKVFTNLHYRKPEVCVMERRLPLPKPNOV52a--------------LELHVASGIRGENADYVEQAIIQTVRTNFPETWMWDLVSVDSSGSANOV52b--------------LELRVASGIRGENADYVEQAIIQTVRTNFPETWMWDLVSVDSSGSANOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hLYLETENYGPMRSVPSRIASSGIRGENADYVEQAIIQTVRTNFPETWMWDLVSVDSSGSANOV52aNLSFLIPDTITQWEASGFCVNGDVGFGISSTTTLEVSQPFFIEIASPFSVVQNEQFDLIVNOV52bNLSFLIPDTITQWEASGFCVNGDVGFGISSTTTLEVSQPFFIEIASPFSVVQNEQFDLIVNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hNLSFLIPDTITQWEASGFCVNGDVGFGISSTTTLEVSQPFFIEIASPFSVVQNEQFDLIVNOV52aNVFSYRNTCVEISVQVEESQNYEANIHTLKINGSEVIQAGGRKTNVWTIIPKKLGKVNITNOV52bNVFSYRNTCVEISVQVEESQNYEANIHTLKINGSEVIQAGGRKTNVWTIIPKKLGKVNITNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hNVFSYRNTCVEISVQVEESQNYEANIHTLKINGSEVIQAGGRKTNVWTIIPKKLGKVNITNOV52aVVAESKQSSACPNEGMEQQKLNWKDTVVQSFLVEPEGIEKERTQSFLICTEGAKASKQGVNOV52bVVAESKQSSACPNEGMEQQKLNWKDTVVQSFLVEPEGIEKERTQSFLICTEGAKASKQGVNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hVVAESKQSSACPNEGMEQQKLNWKDTVVQSFLVEPEGIEKERTQSFLICTEGAKASKQGVNOV52aLDLPNDVVEGSARGFFTVVGDILGLALQNLVVLQMPYGSGEQNAALLASDTYVLDYLKSTNOV52bLDLPNDVVEGSARGFFTVVGDILGLALQNLVVLQMPYGSGEQNAALLASDTYVLDYLKSTNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hLDLPNDVVEGSARGFFTVVGDILGLALQNLVVLQMPYGSGEQNAALLASDTYVLDYLKSTNOV52aEQLTEEVQSKAFFLLSNGYQRQLSFKNSDGSYSVFWQQSQKGSIWLSALTFKTLERMKKYNOV52bEQLTEEVQSKAFFLLSNGYQRQLSFKNSDGSYSVFWQQSQKGSIWLSALTFKTLERMKKYNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hEQLTEEVQSKAFFLLSNGYQRQLSFKNSDGSYSVFWQQSQKGSIWLSALTFKTLERMKKYNOV52aVFIDENVQKQTLIWLSSQQKTSGCFKNDGQLFNHAWEGGDEEDISLTAYVVGMFFEAGLNNOV52bVFIDENVQKQTLIWLSSQQKTSGCFKNDGQLFNHAWEGGDEEDISLTAYVVGMFFEAGLNNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hVFIDENVQKQTLIWLSSQQKTSGCFKNDGQLFNHAWQGGDEEDISLTAYVVGMFFEAGA-NOV52aFTFPALRNALFCLEAALDSGVTNGYNHAILAYAFALAGKEKQVESLLQTLDQSAPKLNNVNOV52bFTFPALRNALFCLEAALDSGVTNGYNHAILAYAFALAGKEKQVESLLQTLDQSAPKLNNVNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52h---------------ALDSGVTNGYNHAILAYAFALAGKEKQVESLLQTLDQSAPKLNNVNOV52aIYWERERKPKTEEFPSFIPWAPSAQTEKSCYVLLAVISRKIPDLTYASKIVQWLAQRMNSNOV52bIYWERERKPKTEEFPSFIPWAPSAQTEKSCYVLLAVISRKIPDLTYASKIVQWLAQRMNSNOV52c------------------------------------------------------------NOV52d------------------------------------------------------------NOV52e------------------------------------------------------------NOV52f------------------------------------------------------------NOV52g------------------------------------------------------------NOV52hIYWERERKPKTEEFPSFIPWAPSAQTEKSCYVLLAVISRKIPDLTYASKIVQWLAQRMNSNOV52aHGGFSSNQTPDDTLFKLYTGQKESFRSSSVGYTLGKANEKKENRRNGGEGSSEIFQVNGHNOV52bHGGFSSNQTPDDTLFKLYTGQKESFRSSSVGYTLGKANEKKENRRNGGEGSSEIFQVNGHNOV52c------------------------TRS---------------------EGSSEIFQVNGHNOV52d------------------------TRS---------------------EGSSEIFQVNGHNOV52e------------------------TRS---------------------EGSSEIFQVNGHNOV52f------------------------TRS---------------------EGSSEIFQVNGHNOV52g------------------------TRS---------------------EGSSEIFQVNGHNOV52hHGGFSSNQDQNT----------VTFSS---------------------EGSSEIFQVNGHNOV52aNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52bNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52cNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52dNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52eNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYDVLLPKKASGFSLSLEIVKNYSSTANOV52fNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52gNRLLVQRSEVTQAPGQYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52hNRLLVQRSEVTQAPGEYTVDVEGHGCTFIQATLKYNVLLPKKASGFSLSLEIVKNYSSTANOV52aFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52bFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52CFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52dFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52eFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52fFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52gFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSTEELENKGQVMKTEVKNDHVLFYLENOV52hFDLTVTLKYTGIRNKSSMVVIDVKMLSGFTPTMSSIEELENKGQVMKTEVKNDHVLFYLENOV52aNVGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSE-------NOV52bNVGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSE-------NOV52cNG-FGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEG----NOV52dNG-FGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEG----NOV52eNG-FGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEG----NOV52fNS---------------NLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEG----NOV52gNS---------------NLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSELEGKGESNOV52hNVGFGRADSFPFSVEQSNLVFNIQPAPAMVYDYYEKEEYALAFYNIDSSSVSE-------NOV52a--NOV52b--NOV52c--NOV52d--NOV52e--NOV52f--NOV52gSTNOV52h--


Further analysis of the NOV52a protein yielded the following properties shown in Table 52C.

TABLE 52CProtein Sequence Properties NOV52aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 8; pos. chg 1; neg. chg 1H-region:length 6; peak value −6.21PSG score:−10.61GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −9.40possible cleavage site: between 45 and 46>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 3Number of TMS(s) for threshold 0.5: 0PERIPHERALLikelihood = 1.11 (at 629)ALOM score: −1.70 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):9.53Hyd Moment(95):7.08G content:0D/E content:2S/T content:1Score: −6.16Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: RKPK (4) at 1307pat7: nonebipartite: nonecontent of basic residues: 8.7%NLS Score: −0.22KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: foundKIVPEPKQL at 596VAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):47.8%: nuclear30.4%: cytoplasmic17.4%: mitochondrial 4.3%: vesicles of secretory system>> prediction for CG59584-03 is nuc (k = 23)


A search of the NOV52a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 52D.

TABLE 52DGeneseq Results for NOV52aNOV52aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABU52373Human GPCR related protein158 . . . 15931436/1436(100%)0.0NOV26b - Homo sapiens, 1436 aa. 1 . . . 14361436/1436(100%)[WO200279398-A2, 10-OCT-2002]ABU52372Human GPCR related protein307 . . . 1593997/1311(76%)0.0NOV26a - Homo sapiens, 1411 aa.164 . . . 14111072/1311(81%)[WO200279398-A2, 10-OCT-2002]ABG05715Novel human diagnostic protein308 . . . 1137742/852(87%)0.0#5706 - Homo sapiens, 1200 aa.30 . . . 819748/852(87%)[WO200175067-A2, 11-OCT-2001]ABG05716Novel human diagnostic protein393 . . . 1137655/767(85%)0.0#5707 - Homo sapiens, 1379 aa.294 . . . 998 662/767(85%)[WO200175067-A2, 11-OCT-2001]AAU81018Human alpha2 macroglobulin306 . . . 1589527/1320(39%)0.0(alpha2M) receptor #1 mature protein -144 . . . 1445798/1320(59%)Homo sapiens, 1451 aa.[WO200192474-A1, 06-DEC-2001]


In a BLAST search of public sequence databases, the NOV52a protein was found to have homology to the proteins shown in the BLASTP data in Table 52E.

TABLE 52EPublic BLASTP Results for NOV52aNOV52aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueP20740Ovostatin precursor306 . . . 1584577/1306 (44%)0.0(Ovomacroglobulin) - Gallus gallus178 . . . 1461844/1306 (64%)(Chicken), 1473 aa.P01023Alpha-2-macroglobulin precursor306 . . . 1589527/1320 (39%)0.0(Alpha-2-M) - Homo sapiens167 . . . 1468798/1320 (59%)(Human), 1474 aa.CAA01532ALPHA 2-MACROGLOBULIN 690-306 . . . 1589525/1320 (39%)0.0730 - Homo sapiens (Human), 1474167 . . . 1468795/1320 (59%)aa.CAA01533ALPHA 2-MACROGLOBULIN 690-306 . . . 1589524/1330 (39%)0.0740 - Homo sapiens (Human), 1484167 . . . 1478792/1330 (59%)aa.P06238Alpha-2-macroglobulin precursor306 . . . 1590520/1314 (39%)0.0(Alpha-2-M) - Rattus norvegicus170 . . . 1467782/1314 (58%)(Rat), 1472 aa.


PFam analysis predicts that the NOV52a protein contains the domains shown in the Table 52F.

TABLE 52FDomain Analysis of NOV52aIdentities/SimilaritiesPfamNOV52a Matchfor theDomainRegionMatched RegionExpect ValueA2M_N110 . . . 763171/707 (24%)6e−62420/707 (59%)A2M 852 . . . 1584258/858 (30%)3.9e−146503/858 (59%)


Example 53

The NOV53 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 53A.

TABLE 53ANOV53 Sequence AnalysisNOV53a, 56008-01SEQ ID NO:11073445 bpDNA SequenceORF Start: ATG at 117ORF Stop: TGA at 2381CACCGCGTGTTCGCGCCTGGTAGAGATTTCTCGAAGACACCAGTGGGCCCGTGTGGAACCAAACCTGCGCGCGTGGCCGGGCCGTGGGACAACGAGGCCGCGGAGACGAAGGCGCAATGGCGAGGAAGTTATCTGTAATCTTGATCCTGACCTTTGCCCTCTCTGTCACAAATCCCCTTCATGAACTAAAAGCAGCTGCTTTCCCCCAGACCACTGAGAAAATTAGTCCGAATTGGGAATCTGGCATTAATGTTGACTTGGCAATTTCCACACGGCAATATCATCTACAACAGCTTTTCTACCGCTATGGAGAAAATAATTCTTTGTCAGTTGAAGGGTTCAGAAAATTACTTCAAAATATAGGCATAGATAAGATTAAAAGAATCCATATACACCATGACCACGACCATCACTCAGACCACGAGCATCACTCAGACCATGAGCGTCACTCAGACCATGAGCATCACTCAGAGCACGAGCATCACTCTGACCATGATCATCACTCTCACCATAATCATGCTGCTTCTGGTAAAAATAAGCGAAAAGCTCTTTGCCCAGACCATGACTCAGATAGTTCAGGTAAAGATCCTAGAAACAGCCAGGGGAAAGGAGCTCACCGACCAGAACATGCCAGTGGTAGAAGGAATGTCAAGGACAGTGTTAGTGCTAGTGAAGTGACCTCAACTGTGTACAACACTGTCTCTGAAGGAACTCACTTTCTAGAGACAATAGAGACTCCAAGACCTGGAAAACTCTTCCCCAAAGATGTAAGCAGCTCCACTCCACCCAGTGTCACATCAAAGAGCCGGGTGAGCCGGCTGGCTGGTAGGAAAACAAATGAATCTGTGAGTGAGCCCCGAAAAGGCTTTATGTATTCCAGAAACACAAATGAAAATCCTCAGGAGTGTTTCAATGCATCAAAGCTACTGACATCTCATGGCATGGGCATCCAGGTTCCGCTGAATGCAACAGAGTTCAACTATCTCTGTCCAGCCATCATCAACCAAATTGATGCTAGATCTTGTCTGATTCATACAAGTGAAAAGAAGGCTGAAATCCCTCCAAAGACCTATTCATTACTGGCACTGGCCGTTGGGACTTTGAGTGGTGATGCTTTTTTACACCTTCTTCCACATTCTCATGCAAGTCACCACCATAGTCATAGCCATGAAGAACCAGCAATGGAAATGAAAAGAGGACCACTTTTCAGTCATCTGTCTTCTCAAAACATAGAAGAAAGTGCCTATTTTGATTCCACGTGGAAGGGTCTAACAGCTCTAGGAGGCCTGTATTTCATGTTTCTTGTTGAACATGTCCTCACATTGATCAAACAATTTAAAGATAAGAAGAAAAAGAATCAGAAGAAACCTGAAAATGATGATGATGTGGAGATTAAGA2GCAGTTGTCCAAGTATGAATCTCAACTTTCAACAAATGAGGAGAAAGTAGATACAGATGATCGAACTGAAGGCTATTTACGAGCAGACTCACAAGAGCCCTCCCACTTTGATTCTCAGCAGCCTGCAGTCTTGGAAGAAGAAGAGGTCATGATAGCTCATGCTCATCCACAGGAAGTCTACAATGAATATGTACCCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGCCAGTCAGACGATCTCATTCACCACCATCATGACTACCATCATATTCTCCATCATCACCACCACCAAAACCACCATCCTCACAGTCACAGCCAGCGCTACTCTCGGGAGGAGCTGAAAGATGCCGGCGTCGCCACTCTGGCCTGGATGGTGATAATGGGTGATGGCCTGCACAATTTCAGCGATGGCCTAGCAATTGGTGCTGCTTTTACTGAAGGCTTATCAAGTGGTTTAAGTACTTCTGTTGCTGTGTTCTGTCATGAGTTGCCTCATGAATTAGGTGACTTTGCTGTTCTACTAAAGGCTGGCATGACCGTTAAGCAGGCTGTCCTTTATAATGCATTGTCAGCCATGCTGGCGTATCTTGGAATGGCAACAGGAATTTTCATTGGTCATTATGCTGAAAATGTTTCTATGTGGATATTTGCACTTACTGCTGGCTTATTCATGTATGTTGCTCTGGTTGATATGGTACCTGAAATGCTGCACAATGATGCTAGTGACCATGGATGTAGCCGCTGGGGGTATTTCTTTTTACAGAATGCTGGGATGCTTTTGGGTTTTGGAATTATGTTACTTATTTCCATATTTGAACATAAAATCGTGTTTCGTATAAATTTCTAGTTAAGGTTTAAATGCTAGAGTAGCTTAAAAAGTTGTCATAGTTTCAGTAGGTCATAGGGAGATGAGTTTGTATGCTGTACTATGCAGCGTTTAAAGTTAGTGGGTTTTGTGATTTTTGTATTGAATATTGCTGTCTGTTACAAAGTCAGTTAAAGGTACGTTTTAATATTTAAGTTATTCTATCTTGGAGATAAAATCTGTATGTGCAATTCACCGGTATTACCAGTTTATTATGTAAACAAGAGATTTGGCATGACATGTTCTGTATGTTTCAGGGAAAAATGTCTTTAATGCTTTTTCAAGAACTAACACAGTTATTCCTATACTGGATTTTAGGTCTCTGAAGAACTGCTGGTGTTTAGGAATAAGAATGTGCATGAAGCCTAAAATACCAAGAAAGCTTATACTGAATTTAAGCAAAGAAATAAAGGAGAAAAGAGAAGAATCTGAGAATTGGGGAGGCATAGATTCTTATAAAAATCACAAAATTTGTTGTAAATTAGAGGGGAGAAATTTAGAATTAAGTATAAAAAGGCAGAATTAGTATAGAGTACATTCATTAAACATTTTTGTCAGGATTATTTCCCGTAAAAACGTAGTGAGCACTTTTCATATACTAATTTAGTTGTACATTTAACTTTGTATAATACAGAAATCTAAATATATTTAATGAATTCAAGCAATATATCACTTGACCAAGAAATTGGAATTTCAAAATGTTCGTGCGGGTATATACCAGATGAGTACAGTGAGTAGTTTTATGTATCACCAGACTGGGTTATTGCCAAGTTATATATCACCAAAAGCTGTATGACTGGATGTTCTGGTTACCTGGTTTACAAAATTATCAGAGTAGTAAAACTTTGATATATATGAGGATATTAAAACTACACTAAGTATCATTTGATTCGATTCAGAAAGTACTTTGATATCTCTCAGTGCTTCAGTGCTATCATTGTGAGCAATTGTCTTTTATATACGGTACTGTAGCCATACTAGGCCTGTCTGTGGCATTCTCTAGATGTTTCTTTTTTACACAATAAATTCCTTATATCAGCTTGNOV53a, CG56008-01SEQ ID NO:1108775 aaProtein SequenceMARKLSVILILTFALSVTNPLHELKAAAFPQTTEKISPNWESGINVDLAISTRQYHLQQLFYRYGENNSLSVEGFRKLLQNIGIDKIKRIHIHHDHDHHSDHEHHSDHERHSDHEHHSEHEHHSDHDHHSHHNHAASGKNKRKALCPDHDSDSSGKDPRNSQGKGAHRPEHASGRRNVKDSVSASEVTSTVYNTVSEGTHFLETIETPRPGKLFPKDVSSSTPPSVTSKSRVSRLAGRKTNESVSEPRKGFMYSRNTNENPQECFNASKLLTSHGMGIQVPLNATEFNYLCPAIINQIDARSCLIHTSEKKAEIPPKTYSLQTAWVGGFIAISIISFLSLLGVILVPLMNRVFFKFLLSFLVALAVGTLSGDAFLHLLPHSHASHHHSHSHEEPAMEMKRGPLFSHLSSQNIEESAYFDSTWKGLTALGGLYFMFLVEHVLTLIKQFKDKKKKNQKKPENDDDVEIKKQLSKYESQLSTNEEKVDTDDRTEGYLRADSQEPSHFDSQQPAVLEEEEVMIAHAHPQEVYNEYVPRGCKNKCHSHFHDTLGQSDDLIHHHHDYHHILHHHHHQNHHPHSHSQRYSREELKDAGVATLAWMVIMGDGLHNFSDGLAIGAAFTEGLSSGLSTSVAVFCHELPHELGDFAVLLKAGMTVKQAVLYNALSAMLAYLGMATGIFIGHYAENVSMWIFALTAGLFMYVALVDMVPENLHNDASDHGCSRWGYFFLQNAGMLLGFGIMLLISIFEHKIVFRINF


Further analysis of the NOV53a protein yielded the following properties shown in Table 53B.

TABLE 53BProtein Sequence Properties NOV53aSignalP analysis:Cleavage site between residues 18 and 19PSORT II analysis:Results Summary:plasma membrane --- Certainty = 0.6400(Affirmative) < succ>Golgi body --- Certainty = 0.4600(Affirmative) < succ>endoplasmic reticulum (membrane) --- Certainty =0.3700(Affirmative) < succ>endoplasmic reticulum (lumen) --- Certainty =0.1000(Affirmative) < succ>Reasoning Steps:>>> MUS belongs to the animal class*** Reasoning Step: 2SRCFLG:1Prelim. Calc. of ALOM (thresh: 0.5) count: 7mostN:5 at i = 4MTOP programImiddle: 12 Chg diff(C-N): −2.5McG:Length of UR: 17Peak Value of UR: 3.09Net Charge of CR: 2McG:Discrim Score: 9.90GvH:Signal Score (−3.5): 2.04Possible site: 20>>> Seems to have a cleavable N-term signal seq.Amino Acid Composition: calculated from 21involving clv.sig in the ALOMREC or not: 1BALOM programcount: 6 value: −11.15 threshold: 0.5INTEGRALLikelihood =Transmembrane−11.15324-340 (313-348)INTEGRALLikelihood =Transmembrane −5.26346-362 (342-369)INTEGRALLikelihood =Transmembrane −4.73681-697 (679-701)INTEGRALLikelihood =Transmembrane −2.44725-741 (723-742)INTEGRALLikelihood =Transmembrane −1.97656-672 (656-673)INTEGRALLikelihood =Transmembrane −1.59422-438 (421-438)PERIPHERALLikelihood =  3.45modified ALOM score: 2.83>>> Likely a Type IIIa membrane protein (clv)Gavel:Bound.Mitoch.Preseq. R-2 motif: 5 ARKLSVmtdisc (mit) Status: positive (3.31)*** Reasoning Step: 3KDELCount: 02nd signal for mitochondria (apolar)(5)from: 5 to: 17 Score: 8.5SKL motif: pos: 399(752), count: 3 SHLpox modified by SKL scr: 0.1PoxaacScore: 2.67aac not from the N-term. scr modified>>> POX Status: notclr>>> lys: −1.27 Status: negativeNuc-4 pos: 443 (5) KKKK>>> Nuclear Signal. Status: negative (0.00)


A search of the NOV53a protein against the Geneseq and public databases, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 53C. In a BLAST search of public sequence databases, the NOV53a protein was found to have homology to the proteins shown in the BLASTP data in Table 53D.

TABLE 53CGeneseq and Public Results for NOV53aNOV53aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAM51198Human breast cancer 4 gene32 . . . 773735/742 (99%)(BCR4)-encoded 1 . . . 736736/742 (99%)Q13433ESTROGEN REGULATED LIV-132 . . . 786748/755 (99%)PROTEIN. 1 . . . 749749/755 (99%)AAE07205Human LIV-1 protein32 . . . 773735/742 (99%) 1 . . . 736736/742 (99%)


PFam analysis predicts that the NOV53a protein contains the domains shown in the Table 53E.

TABLE 53EDomain Analysis of NOV53aScore forNOV53a Matchthe MatchedExpectPfam DomainRegionRegionValuePeptidase_C4251 . . . 2664.63.4e−56Osteopontin 4 . . . 300−85.10.95


Example 54

The NOV54 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 54A.

TABLE 54ANOV54 Sequence Analysis+TL,1NOV54a, CG59905-01SEQ ID NO:11093145 bpDNA SequenceORF Start: ATG at 34ORF Stop: TGA at 3046ATTTTACTTGCTGGATCTTCAGCCTTGACCTGTATGGCAAATGGCTTATGGGACCGATCCCTGCCCAAGTGTTTGGCTATATCCTGTGGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCCGTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGCCAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGAAATAATCCTGGATTCTGTGGTGATCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAGAGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACGTGTTTGCTCAATGGGTCATGGTCAGGACTGCAGCCGGTGTGTGAGGCCGTGTCCTGTGGCAACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGCTCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGCACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACTGCACAATTATAAGTTGTGGGGATCCAGGCACACTAGCAAATGGCATCCAGTTTGGGACCGACTTCACCTTCAACAAGACTGTGAGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGTACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGCCGTGCTGTGTCCTCAGCCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATAAGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGTCGCGGGGTGTGGAAAGGAGAGATCCCCAGTGTCTGCCTGTGTTCTGCGGAGACCCTGGCATCCCCGCAGAAGGGCGACTTAGTGGGAAAAGTTTCACCTATAAGTCCGAAGTCTTCTTCCAGTGCAAATCTCCATTTATACTCGTGGGATCCTCCAGAAGAGTCTGCCAAGCTGACGGCACGTGGAGCGGCATACAACCCACCTGCATTGGTAATAATTATCATACAGCTCTGGGGATACCTGGGAGTATTTGGAGATGAGGACGCTTCATTCCGAAATTGGGTCATTTGTGATTACATAGAAAGTGTTTCCATAGACAGTTTTCTGTACAAGGTTGGAAGCACGGTTTTTTTCAGGTGCAGAAAAGGCTACCATATTCAAGGTTCCACGACTCGCACCTGCCTTGCCAATTTAACATGGAGTGGGATACAGACCGAATGTATACCTCATGCCTGCAGACAGCCAGAAACCCCGGCACACGCGGATGTGAGAGCCATCGATCTTCCTACTTTCGGCTACACCTTAGTGTACACCTGCCATCCAGGCTTTTTCCTCGCAGGGGGATCTGAGCACAGAACATGTAAAGCAGACATGAAATGGACAGGAAAGTCGCCTGTGTGTAAAATTCCTTCAGATGTCTTTTTCGTCAATTCACTGTGGAAGGGGTATTATGAATATTTAGGGAAAAGACAACCCGCCACTCTAACTGTTGACTGGTTCAATGCAACAAGCAGTAAGGTGAATGCCACCTTCAGCGAAGCCTCGCCAGTGGAGCTGAAGTTGACAGGCATTTACAAGAAGGAGGAGGCCCACTTACTCCTGAAAGCTTTTCAAATTAAAGGCCAGGCAGATATTTTTGTAAGCAAGTTCGAAAATGACAACTGGGGACTAGATGGTTATGTGTCATCTGGACTTGAAAGAGGAGGATTTACTTTTCAAGGTGACATTCATGGAAAAGACTTTGGAAAATTTAAGCTAGAAAGGCAAGGATGGGTCACAATATTCTTGAGCCTATTTCTTCATCTTAAATCTCAGTATAGAAGTTCCCAAGGTTGTTACGAGATTGAGAGGCCACATCCTTTAAACCCAGATCAAGACTCTTCCAGTCATTACCACGGCACCAGCAGTGGCTCTGTGGCGGCTGCCATTCTGGTTCCTTTCTTTGCTCTAATTTTATCAGGGTTTGCATTTTACCTCTACAAACACAGAACGAGACCAAAAGTTCAATACAATGGCTATGCTGGGCATGAAAACAGCAATGGACAAGCATCGTTTGAAAACCCCATGTATGATACAAACTTAAAACCCACAGAAGCCAAGGCTAAAACCACACACGGGCTGCTCACGTCTCTGGACTCCAAGAGAAAGATGGTTATAACCCTGGCAGAGTCTTTACCCGCCCTGCGAACCTGCCTGTTGATCTGCTTCCCACTCCTCTTTAGACTTGCTCGTGAATCTTCAGCACAGCCATTTGTTTTGAGTATTCAAACACATAAAAAGATATCTGGCAACTTCCCACTTCTTATGACTTCTCAGACACCTTGTAACGGAGCTTCCCTTGGAGGTGGACAAACTTCTCACTCACAAGAGAGACGGAGAGCAACAGAGAGAGATGGGCAGGGATTGATTCGAGGATTGGCTGACTCGATTATGGAGGGTGAGAAGTCCCAGGACAGGCCGTCTGCAAGGCGTAAACCCAGGGAAGCTGCTGGTATGGCTCGGTTCAAGTCCAAAGGCCTCAGCACCAAGGAAACCAAGGTGATAACTCTCAGTTCGGGTCAAAGTCCTGGGAGTCTGCAAGGCCTCTCATTGAGTAAGTCTCACAAGATCCGATGGTTTTATAAAGGGCAGTTCCCCTGCACAAGCTCTCTTCTCTGCCACCACGCGTTTGCTGTTCATTCACCTTTCACCATGATTGTGAGGCCTCCCAGCCATGTGGAACTGTGCAAAGAGGATGTTCAAAGGGCATCTTGGACCAGGAGGAGGAAGCCCATTGTGAGGATGGCAAAGAACAATAAATGGAAGGCCCGTGGAACCCTGAGCGGCTGCCCCATCACTGTCCTCTCCATCCTTTCCATGCTTCCCATGGTGCATGGCTCTCAGGCTCTCCAAGCACCATGAGCTCCTGGCGCGTATGGCCTGGCCCCTGAGTGTGGAGTCTGTGGACTTTAAATGTGCCTTTACTCAGCCAGCATCTCCCTGCTGGGTGCGATGTTGNOV54a, CG59905-01SEQ ID NO:11101004 aaMW at 109752.7 kDProtein SequenceMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGAVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTKSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGTLFSSSVTYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTVSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSISYSCMDGYQLSHSAILSCEGRGVWKGEIPSVCLCSAETLASPQKGDLVGKVSPISPKSSSSANLHLYSWDPPEESAKLTARGAAYNPPALVIIIIQLWGYLGVFGDEDASFRNWVICDYIESVSIDSFLYKVGSTVFFRCRKGYEIQGSTTRTCLANLTWSGIQTECIPHACRQPETPAHADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKIPSDVFFVNSLWKGYYEYLGKRQPATLTVDWFNATSSKVNATFSEASPVELKLTGIYKKEEAHLLLKAFQIKGQADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFKLERQGWVTIFLSLFLHLKSQYRSSQGCYEIERPHPLNPDQDSSSHYHGTSSGSVAAAILVPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMYDTNLKPTEAKAKTTHGLLTSLDSKRKMVITLAESLPALRTCLLICFPLLFRLARESSAQPFVLSIQTHKKISGNFPLLMTSQTPCNGASLGGGQTSHSQERRRATERDGQGLIRGLADSIMEGEKSQDRPSARRKPREAAGMARFKSKGLSTKETKVITLSSGQSPGSLQGLSLSKSHKIRWFYKGQFPCTSSLLCHHAFAVHSPFTMIVRPPSHVELCKEDVQRASWTRRRKPIVRMAKNNKWKARGTLSGCPITVLSTLSMLPMVHGSQALQAPNOV54b, CG59905-03SEQ ID NO:111110989 bpDNA SequenceORF Start: ATG at 286ORF Stop: TGA at 10987CCTTCCTGCCCGGCTCGGCCGGCGCTCGTCCCCGGCCCCGGCCCCGCCAGCCCGGGTCTCCGCGCTCGGAGCAGCTCAGCCCTGCAGTGGCTCGGGACCCGATGCTATGAGAGGGAAGCGAGCCGGGCGCCCAGACCTTCAGGAGGCGTCGGATGCGCGGCGGGTCTTGGGACCGGGCTCTCTCTCCGGCTCGCCTTGCCCTCGGGTGATTATTTGGCTCCGCTCATAGCCCTGCCTTCCTCGGAGGAGCCATCGGTGTCGCGTGCGTGTGGAGTATCTGCAGACATGACTGCGTGGAGGAGATTCCAGTCGCTGCTCCTGCTTCTCGGGCTGCTGGTGCTGTGCGCGAGGCTCCTCACTGCAGCGAAGGGTCAGAACTGTGGAGGCTTAGTCCAGGGTCCCAATGGCACTATTGAGAGCCCAGGGTTTCCTCACGGGTATCCGAACTATGCCAACTGCACCTGGATCATCATCACGGGCGAGCGCAATAGGATACAGTTGTCCTTCCATACCTTTGCTCTTGAAGAAAATTTTGATATTTTATCAGTTTACGATGGACAGCCTCAACAAGGGAATTTAAAAGTGAGATTATCGGGATTTCAGCTGCCCTCCTCTATAGTGAGTACAGGATCTATCCTCACTCTGTGGTTCACGACAGACTTCGCTGTGAGTGCCCAAGGTTTCAAAGCATTATATGAAGTTTTACCTAGCCACACTTGTGGAAATCCTGGAGAAATCCTGAAAGGAGTTCTGCATGGAACGAGATTCAACATAGGAGACAAAATCCGGTACAGCTGCCTCCCTGGCTACATCTTGGAAGGCCACGCCATCCTGACCTGCATCGTCAGCCCAGGAAATGGTGCATCGTGGGACTTCCCAGCTCCCTTTTGCAGAGCTGAGGGAGCCTGCGGAGGAACCTTACGCGGGACCAGCAGCTCCATCTCCAGCCCGCACTTCCCTTCAGAGTACGAGAACAACGCGGACTGCACCTGGACCATTCTGGCTGAGCCCGGGGACACCATTGCGCTGGTCTTCACTGACTTTCAGCTAGAAGAAGGATATGATTTCTTAGAGATCAGTGGCACGGAAGCTCCATCCATATGGCTAACTGGCATGAACCTCCCCTCTCCAGTTATCAGTAGCAAGAATTGGCTACGACTCCATTTCACCTCTGACAGCAACCACCGACGCALAGGATTTAACGCTCAGTTCCAAGTGAAAAAGGCGATTGAGTTGAAGTCAAGAGGAGTCAAGATGCTGCCCAGCAAGGATGGAAGCCATAAAAACTCTGTCTTGAGCCAAGGAGGTGTTGCATTGGTCTCTCACATGTGTCTAGATCCTGGGATTCCAGAAAATGGTAGAAGAGCAGGTTCCGACTTCAGTAGGGTTGGTGCAAATGTACAGTTTTCATGTGAGGACAATTACGTGCTCCAGGGATCTAAAAGCATCACCTGTCAGAGAGTTACAGAGACGCTCGCTGCTTGGAGTGACCACAGGCCCATCTGCCGAGCGAGAACATGTGGATCCAATCTGCGTGGGCCCAGCGGCGTCATTACCTCCCCTAATTATCCGGTTCAGTATGAAGATAATGCACACTGTGTGTGGGTCATCACCACCACCGACCCGGACAAGGTCATCAAGCTTGCCTTTGAAGAGTTTGAGCTGGAGCGAGGCTATGACACCCTGACGGTTGGTGATGCTGGGAAGGTGGGAGACACCAGATCGGTCTTGTACGTGCTCACGGGATCCAGTGTTCCTGACCTCATTGTGAGCATGAGCAACCAGATGTGGCTACATCTGCAGTCGGATGATAGCATTGGCTCACCTGGGTTTAAAGCTGTTTACCAAGAAATTGAAAAGGGAGGGTGTGGGGATCCTGGAATCCCCGCCTATGGGAAGCGGACGGGCAGCAGTTTCCTCCATGGAGATACACTCACCTTTGAATGCCCGGCGGCCTTTGAGCTGGTGGGGGAGAGAGTTATCACCTGTCAGCAGAACAATCAGTGGTCTGGCAACAAGCCCAGCTGTGTATTTTCATGTTTCTTCAACTTTACGGCATCATCTGGGATTATTCTGTCACCAAATTATCCAGAGGAATATGGGAACAACATGAACTGTGTCTGGTTGATTATCTCGGAGCCAGGAAGTCGAATTCACCTAATCTTTAATGATTTTGATGTTGAGCCTCAATTTGACTTTCTCGCGGTCAAGGATAATGGCATTTCTGACATAACTGTCCTGGGTACTTTTTCTGGCAATGAAGTGCCTTCCCAGCTGGCCAGCAGTGGGCATATAGTTCGCTTGGAATTTCAGTCTGACCATTCCACTACTGGCAGAGGGTTCAACATCACTTACACCACATTTGGTCAGAATGAGTGCCATGATCCTGGCATTCCTATAAACGGACGACGTTTTGGTGACAGGTTTCTACTCGGGAGCTCGGTTTCTTTCCACTGTGATGATGGCTTTGTCAAGACCCAGGGATCCGAGTCCATTACCTGCATACTGCAAGACGGGAACGTGGTCTGGAGCTCCACCGTGCCCCGCTGTGAAGCTCCATGTGGTGGACATCTGACAGCGTCCAGCGGAGTCATTTTGCCTCCTGGATGGCCAGGATATTATAAGGATTCTTTACATTGTGAATGGATAATTGAAGCAAAACCAGGCCACTCTATCAAAATGACTTTTGACAGATTTCAGACAGAGGTCAATTATGACACCCCAGTTCCTCATCAGCACCGGGAACTTCATGTACCTGCTGTTCACCACTGACAACAGCCGCTCCAGCATCGGCTTCCTCATCCACTATGAGAGTGTGACGCTTGAGTCGGATTCCTGCCTGGACCCGGGCATCCCTGTGAACGGCCATCGCCACGGTGGAGACTTTGGCATCAGGTCCACAGTGACTTTCAGCTGTGACCCGGGGTACACACTAAGTGACGACGAGCCCCTCGTCTGTGAGAGGAACCACCAGTGGAACCACGCCTTGCCCAGCTGCGACGCTCTATGTGGAGGCTACATCCAAGGGAAGAGTGGAACAGTCCTTTCTCCTGGGTTTCCAGATTTTTATCCAAACTCTCTAAACTGCACGTGGACCATTGAAGTGTCTCATGGGAAAGGAGTTCAAATGCTCTTTCACACCTTTCATCTTGAGAGTTCCCACGACTATTTACTGATCACAGAGGATGGAAGTTTTTCCGAGCCCGTTGCCAGGCTCACCGGGTCGGTGTTGCCTCATACGATCAAGGCAGGCCTGTTTGGAAACTTCACTGCCCAGCTTCGGTTTATATCAGACTTCTCAATTTCGTACGAGGGCTTCAATATCACATTTTCAGAATATGACCTGGAGCCATGTGATGATCCTGGAGTCCCTGCCTTCAGCCGAAGAATTGGTTTTCACTTTGGTGTGGGAGACTCTCTGACGTTTTCCTGCTTCCTGGGATATCGTTTAGAAGGTGCCACCAAGCTTACCTGCCTGGGTGGGGGCCGCCGTGTGTGGAGTGCACCTCTGCCAAGGTGTGTGGCCGAATGTGGAGCAAGTGTCAAAGGAAATGAAGGAACATTACTGTCTCCAAATTTTCCATCCAATTATGATAATACCCATGAGTGTATCTATAAAATAGAAACAGAAGCCGGCAAGGGCATCCACCTTAGAACACGAAGCTTCCAGCTGTTTGAAGGAGATACTCTAAAGGTATATGATGGAAAAGACAGTTCCTCACGTCCACTGGGCACGTTCACTAAAAATGAACTTCTGGGGCTGATCCTAAACAGCACATCCAATCACCTATGGCTAGAGTTCAACACCAATGGATCTGACACCGACCAAGGTTTTCAACTCACCTATACCAGTTTTGATCTGGTAAAATGTGAGGATCCGGGCATCCCTAACTACGGCTATAGGATCCGTGATGAAGGCCACTTTACCGACACTGTAGTTCTGTACAGTTGCAACCCGGGGTACGCCATGCATGGCAGCAACACCCTGACCTGTTTGAGTGGAGACAGGAGAGTGTGGGACAAACCACTACCTTCGTGCATAGCGGAATGTGGTGGTCAGATCCATGCAGCCACATCAGGACGAATATTGTCCCCTGGCTATCCAGCTCCGTATGACAACAACCTCCACTGCACCTGGATTATAGAGGCAGACCCAGGAAAGACCATTAGCCTCCATTTCATTGTTTTCGACACGGAGATGGCTCACGACATCCTCAAGGTCTGGGACGGGCCGGTGGACAGTGACATCCTGCTGAAGGAGTGGAGTGGCTCCGCCCTTCCGGAGGACATCCACAGCACCTTCAACTCACTCACCCTGCAGTTCGACAGCGACTTCTTCATCAGCAAGTCTGGCTTCTCCATCCAGTTCTCCACCTCAATTGCAGCCACCTGTAACGATCCAGGTATGCCCCAAAATGGCACCCGCTATGGAGACAGCAGAGAGGCTGGAGACACCGTCACATTCCAGTGTGACCCTGGCTATCAGCTCCAAGGACAAGCCAAAATCACCTGTGTGCAGCTGAATAACCGGTTCTTTTGGCAACCAGACCCTCCTACATGCATAGCTGCTTGTGGAGGGAATCTGACGGGCCCAGCAGGTGTTATTTTGTCACCCAACTACCCACAGCCGTATCCTCCTGGGAAGGAATGTGACTGGAGAGTAAAAGTGAACCCGGACTTTGTCATCGCCTTGATATTCAAAAGTTTCAACATGGAGCCCAGCTATGACTTCCTACACATCTATGAAGGGGAAGATTCCAACAGCCCCCTCATTGGGAGTTACCAGGGCTCTCAGGCCCCAGAAAGAATAGAGAGTAGCGGAAACAGCCTGTTTCTGGCATTTCGGAGTGATGCCTCCGTGGGCCTTTCAGGGTTCGCCATTGAATTTAAAGAGAAACCACGGGAAGCTTGTTTTGACCCAGGAAATATAATGAATGGGACAAGAGTTGGAACAGACTTCAAGCTTGGCTCCACCATCACCTACCAGTGTGACTCTGGCTATAAGATTCTTGACCCCTCATCCATCACCTGTGTGATTGGGGCTGATGGGAAACCCTCCTGGGACCAAGTGCTGCCCTCCTGCAATGCTCCCTGTGGAGGCCAGTACACGGGATCAGAAGGGGTAGTTTTATCACCAAACTACCCCCATAATTACACAGCTGGTCAAATATGCCTCTATTCCATCACGGTACCAAAGGAATTCGTGGTCTTTGGACAGTTTGCCTATTTCCAGACAGCCCTGAATGATTTGGCAGAATTATTTGATGGAACCCATGCACAGGCCAGACTTCTCAGCTCACTCTCGGGGTCTCACTCAGGGGAAACATTGCCCTTGGCTACGTCAAATCAAATTCTGCTCCGATTCAGTGCAAAGAGCGGTGCCTCTGCCCGCGGCTTCCACTTCGTGTATCAAGCTGTTCCTCGTACCAGTGACACCCAATGCAGCTCTGTCCCCGAGCCCAGATACGGAAGGAGAATTGGTTCTGAGTTTTCTGCCGGCTCCATCGTCCGATTCGAGTGCAACCCGGGATACCTGCTTCAGGGTTCCACGGCGCTCCACTGCCAGTCCGTGCCCAACGCCTTGGCACAGTGGAACGACACGATCCCCAGCTGTGTGGTACCCTGCAGTGGCAATTTCACTCAACGAAGAGGTACAATCCTGTCCCCCGGCTACCCTGAGCCATACGGAAACAACTTGAACTGTATATGGAAGATCATAGTTACGGAGGGCTCGGGAATTCAAGATCCAAGTGATCAGTTTTGCCACGGAGCAGAACTGGGACTTCCCTTTGAGATCCACGATGGTGGGGATGTGACCGCACCCAGACTGGGAAGCTTCTCAGGCACCACAGTACCGGCACTGCTGAACAGTACTTCCAACCAACTCTACCTGCATTTCCAGTCTGACATTAGTGTGGCAGCTGCTGGTTTCCACCTGGAATACAAAACTGTAGGTCTTGCTGCATGCCAAGAACCAGCCCTCCCCAGCAACAGCATCAAAATCGGAGATCGGTACATGGTGAACGACGTGCTCTCCTTCCAGTGCGAGCCCGGGTACACCCTGCAGGGCCGTTCCCACATTTCCTGTATGCCAGGGACCGTTCGCCGTTGGAACTATCCGTCTCCCCTGTGCATTGCAACCTGTGGAGGGACGCTGAGCACCTTGGGTGGTGTGATCCTGAGCCCCGGCTTCCCAGGTTCTTACCCCAACAACTTAGACTGCACCTGGAGGATCTCATTACCCATCGGCTATGGTGCACATATTCAGTTTCTGAATTTTTCTACCGAAGCTAATCATGACTTCCTTGAAATTCAAAATGGACCTTACCACACCAGCCCCATGATTGGACAATTTAGCGGCACGGATCTCCCCGCGGCCCTGCTGAGCACAACGCATGAAACCCTCATCCACTTTTATAGTGACCATTCGCAAAACCGGCAAGGATTTAAACTTGCTTACCAAGCCTATGAATTACAGAACTGTCCAGATCCACCCCCATTTCAGAATGGGTACATGATCAACTCGGATTACAGCGTGGGGCAATCAGTATCTTTCGAGTGTTATCCTGGGTACATTCTAATAGGCCATCCTGTCCCCACTTGTCAGCATGGGATCAACAGAAACTGGAACTACCCTTTTCCAAGATGTGATGCCCCTTGTGGGTACAACGTAACTTCTCAGAACGGCACCATCTACTCCCCTGGCTTTCCTGATGAGTATCCGATCCTGAAGGACTGCATTTGGCTCATCACGGTGCCTCCAGGGCACGGAGTTTACATCAACTTCACCCTGTTACAGATTCAGTGGCAACACAGCCCTCGAAACGGCGTATAGCTCCACCAACCAAGTCCTGCTCAAGTTCCACAGCGACTTTTCAAATGGAGGCTTCTTTGTCCTCAATTTCCACGCATTTCAGCTCAAGAAATGTCAACCTCCCCCAGCGGTTCCACAGGCAGAAATGCTTACTGAGGATGATGATTTCGAAATAGGAGATTTTGTGAAGTACCAGTGCCACCCCGGGTACACCTTGGTGGGGACCGACATTCTGACTTGCAAGCTCAGTTCCCAGTTGCAGTTTGAGGGTTCTCTCCCAACATGTGAAGCACAATGCCCAGCAAATGAAGTCCGGACTGGATCATCGGGAGTCATTCTCAGTCCAGGGTATCCGGGTAATTATTTTAACTCCCAGACTTGCTCTTGGAGTATTACTGGAAGTGTTTGATGGTTCTTCTGGGCAAAGTCCTCTGCTAGTAGTCTTAAGTGGGAATCATACTGAACAATCAAATTTTACAAGCAGGAGTAATCAGTTATATCTCCGCTGGTCCACTGACCATGCCACCAGTAAGAAAGGATTCAAGATTCGCTATGCAGCACCTTACTGCAGTTTGACCCACCCCCTGAAGAATGGGGGTATTCTAAACAGGACTGCAGGAGCGGTTGGAAGCAAAGTGCATTATTTTTGCAAGCCTGGATACCGAATGGTCGGCCACAGCAATGCAACCTGTAGACGAAACCCACTTGGCATGTACCAGTGGGACTCCCTCACGCCACTCTGCCAGGCTGTGTCCTGTGGAATCCCAGAATCCCCAGGAAACGGTTCATTTACCGGGAACGAGTTCACTTTGGACAGTAAAGTGGTCTATGAATGTCATGAAGGCTTCAAGCTTGAATCCAGCCAGCAAGCAACAGCCGTGTGTCM~GAAGATGGGCTGTGGAGTAACAAGGGGAAGCCGCCCATGTGTAAGCCGGTCGCTTGCCCCAGCATTGAAGCTCAGCTCTCAGAACATGTCATCTGGAGGCTGGTTTCAGGATCCTTGAATGAGTACGGTGCTCAAGTATTGCTGAGCTGCAGTCCTGGTTACTACTTAGAAGGCTGGAGGCTCCTGCGGTGCCAGGCCAATGGGACGTGGAACATAGGAGATGAGAGGCCAAGCTGTCGAGTTATCTCGTGTGGAAGCCTTTCCTTTCCCCCAAATGGCAACAAGATTGGAACGTTGACAGTTTATGGGGCCACAGCTATATTTACGTGCAACACCGGCTACACGCTTGTGGGGTCTCATGTCAGAGAGTGCTTGGCAAATGGGCTCTGGAGCGGCAGCGAAACTCGATGTTTAGCTGGCCACTGCGGTTCCCCAGACCCGATTGTGAACGGTCACATTAGTGGAGATGGCTTCAGTTACAGAGACACGGTGGTTTACCAGTGCAATCCTGGTTTCCGGCTTGTGGGAACTTCCGTGAGGATATGCCTGCAAGACCACAAGTGGTCTGGACAAACGCCTGTCTGTGTCCGTATCACATGTGGTCACCCTGGAAACCCTGCCCACGGATTCACTAATGGCAGTGAGTTCAACCTGAATGATGTCGTGAATTTCACCTGCAACACGGGCTATTTGCTGCAGGGCGTGTCTCGAGCCCAGTGTCGGAGCAACGGCCAGTGGAGTAGCCCTCTGCCCACGTGTCGAGTGGTGAACTGTTCTGATCCAGGCTTTGTGGAAAATGCCATTCGTCACGGGCAACAGAACTTCCCTGAGAGTTTTGAGTATGGAATGAGTATCCTGTACCATTGCAAGAAGGGATTTTACTTGCTGGGATCTTCAGCCTTGACCTGTATGGCAAATGGCTTATGGGACCGATCCCTGCCCAAGTGTTTGGCTATATCGTGTGGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCCGTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGCCAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGAAATAATCCTGGATTCTGTGGTGATCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAGAGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACGTGTTTGCTCAATGGGTCATGGTCAGGACTGCAGCCGGTGTGTGAGGCCGTGTCCTGTGGCAACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGCTCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGCACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACCTTCAACAAGACTGTGAGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGTACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGCCGTGCTGTGTCCTCAGCCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATAAGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGTCGCGGGGTGTGGAAAGGAGAGATCCCCCAGTGTCTCGCTGTGTTCTGCGGAGACCCTGGCATCCCCGCAGAAGGGCGACTTAGTGGGAAAAGTTTCACCTATAAGTCCGAAGTCTTCTTCCAGTGCAAATCTCCATTTATACTCGTGGGATCCTCCAGAAGAGTCTGCCAAGCTGACGGCACGTGGAGCGGCATACAACCCACCTGCATTGGTGATCCTGCTCATAACACCTGCCCAGACCCTGGTACGCCACACTTTGGAATACAGAATAGCTCCAGAGGCTATGAGGTTGGAAGCACGGTTTTTTTCAGGTGCAGAAAAGGCTACCATATTCAAGGTTCCACGACTCGCACCTGCCTTGCCAATTTAACATGGAGTGGGATACAGACCGAATGTATACGTCATGCCTGCAGACAGCCAGAAACCCCGGCACACGCGGATGTGAGAGCCATCGATCTTCCTACTTTCGGCTACACCTTAGTGTACACCTGCCATCCAGGCTTTTTCCTCGCAGGGGGATCTGAGCACAGAACATGTAAAGCAGACATGAAATGGACAGGCTTCAGATGTCTTTTTCGTCAATTCACTGTGGAAGGGGTATTATGAATATTTAGGGAAAAGACAACCCGCCACTCTAACTGTTGACTGGTTCAATGCAACAAGCAGTAAGGTGAATGCCACCTTCAGCGAAGCCTCGCCAGTGGAGCTGAAGTTGACAGGCATTTACAAGAAGGAGGAGGCCCACTTACTCCTGAAAGCTTTTCAAATTAAAGGCCAGGCAGATATTTTTGTAAGCAAGTTCGAAAATGACAACTGGGGACTAGATGGTTATATTTAAGCTAGAAAGGCAAGATCCTTTAAACCCAGATCAAGACTCTTCCAGTCATTACCACGGCACCAGCAGTGGCTCTGTGGCGGCTGCCATTCTGGTTCCTTTCTTTGCTCTAATTTTATCAGGGTTTGCATTTTACCTCTACAAACACAGAACGAGACCAAAAGTTCAATACAATGGCTATGCTGGGCATGAAAACAGCAATGGACAAGCATCGTTTGAAAACCCCATGTATGATACAAACTTAAAACCCACAGAAGCCAAGGCTGTGAGGTTTGACACAAGTGTGAACACAGTCTGTACAGTGGTATGANOV54b, CG59905-03SEQ ID NO:11123567 aaMW at 389007.4 kDProtein SequenceMTAWRRFQSLLLLLGLLVLCARLLTAAKGQNCGGLVQGPNGTIESPGFPHGYPNYANCTWIIITGERNRIQLSFHTFALEENFDILSVYDGQPQQGNLKVRLSGFQLPSSIVSTGSILTLWFTTDFAVSAQGFKALYEVLPSHTCGNPGEILKGVLHGTRFNIGDKIRYSCLPGYILEGHAILTCIVSPGNGASWDFPAPFCRAEGACGGTLRGTSSSISSPHFPSEYENNADCTWTILAEPGDTIALVFTDFQLEEGYDFLEISGTEAPSIWLTGMNLPSPVISSKNWLRLHFTSDSNHRRKGFNAQFQVKKAIELKSRGVKMLPSKDGSHKNSVLSQGGVALVSHMCLDPGIPENGRPAGSDFSRVGANVQFSCEDNYVLQGSKSITCQRVTETLAAWSDHRPICRARTCGSNLRGPSGVITSPNYPVQYEDNAHCVWVITTTDPDKVIKLAFEEFELERGYDTLTVGDAGKVGDTRSVLYVLTGSSVPDLIVSMSNQMWLHLQSDDSIGSPGFKAVYQEIEKGGCGDPGIPAYGKRTGSSFLHGDTLTFECPAAFELVGERVITCQQNNQWSGNKPSCVFSCFFNFTASSGIILSPNYPEEYGNNMNCVTTGRGFNITYTTFGQNECHDPGIPINGRRFGDRFLLGSSVSFHCDDGFVKTQGSESITCILQDGNVVWSSTVPRCEAPCGGHLTASSGVILPPGWPGYYKDSLHCEWIIEAKPGHSIKMTFDRFQTEVNYDTLEVRDGPASSSPLIGEYHGTQAPQFLISTGNFMYLLFTTDNSRSSIGFLIHYESVTLESDSCLDPGIPVNGHRHGGDFGIRSTVTFSCDPGYTLSDDEPLVCERNHQWNHALPSCDALCGGYIQGKSGTVLSPGFPDFYPNSLNCTWTIEVSHGKGVQMIFHTFHLESSHDYLLITEDGSFSEPVARLTGSVLPHTIKAGLFGNFTAQLRFISDFSISYEGFNITFSEYDLEPCDDPGVPAFSRRIGFHFGVGDSLTFSCFLGYRLEGATKLTCLGTLKVYDGKDSSSRPLGTFTKNELLGLILNSTSNHLWLEFNTNGSDTDQGFQLTYTSFDLVKCEDPGIPNYGYRIRDEGHFTDTVVLYSCNPGYAMHGSNTLTCLSGDRRVWDKPLPSCIAECGGQIHAATSGRILSPGYPAPYDNNLHCTWIIEADPGKTISLHFIVFDTEMAHDILKVWDGPVDSDILLKEWSGSALPEDIHSTFNSLTLQFDSDFFISKSGFSIQFSTSIAATCNDPGMPQNGTRYGDSREAGDTVTFQCDPGYQLQGQAKITCVQLNNRFFWQPDPPTCIAACGGNLTGPAGVILSPNYPQPYPPGKECDWRVKVNPDFVIALIFKSFNMEPSYDFLHIYEGEDSNSPLIGSYQGSQAPERIESSGNSLFLAFRSDASVGLSGFAIEFKEKPREACFDPGNIMNGTRVGTDFKLGSTITYQCDSGYKILDPSSITCVIGADGKPSWDQVLPSCNAPCGGQYTGSEGVVLSPNYPHNYTAGQICLYSITVPKEFVVFGQFAYFQTALNDLAELFDGTHAQARLLSSLSGSHSGETLPLATSNQILLRFSAKSGASARGFHFVYQAVPRTSDTQCSSVPEPRYGRRIGSEFSAGSIVRFECNPGYLLQGSTALHCQSVPNALAQWNDTIPSCVVPCSGNFTQRRGTILSPGYPEPYGNNLNCIWKIIVTEGSGIQDPSDQFCHGAELGLPFEIHDGGDVTAPRLGSFSGTTVPALLNSTSNQLYLHFQSDISVAAAGFHLEYKTVGLAACQEPALPSNSIKIGDRYMVNDVLSFQCEPGYTLQGRSHISCMPGTVRRWNYPSPLCIATCGGTLSTLGGVILSPGFPGSYPNNLDCTWRISLPIGYGAHIQFLNFSTEANHDFLEIQNGPYHTSPMIGQFSGTDLPAALLSTTHETLIHFYSDHSQNRQGFKLAYQAYELQNCPDPPPFQNGYMINSDYSVGQSVSFECYPGYILIGHPVLTCQHGINRNWNYPFPRCDAPCGYNVTSQNGTIYSPGFPDEYPILKDCIWLITVPPGHGVYINFTLLQTEAVNDYIAVWDGPDQNSPQLGVFSGNTALETAYSSTNQVLLKFHSDFSNGGFFVLNFHAFQLKKCQPPPAVPQAEMLTEDDDFEIGDFVKYQCHPGYTLVGTDILTCKLSSQLQFEGSLPTCEAQCPANEVRTGSSGVILSPGYPGNYFNSQTCSWSIKVEPNYNITIFVDTFQSEKQFDALEVFDGSSGQSPLLVVLSGNHTEQSNFTSRSNQLYLRWSTDHATSKKGFKIRYAAPYCSLTHPLKNGGILNRTAGAVGSKVHYFCKPGYRMVGHSNATCRRNPLGMYQWDSLTPLCQAVSCGIPESPGNGSFTGNEFTLDSKVVYECHEGFKLESSQQATAVCQEDGLWSNKGKPPMCKPVACPSIEAQLSEHVIWRLVSGSLNEYGAQVLLSCSPGYYLEGWRLLRCQANGTWNIGDERPSCRVISCGSLSFPPNGNKIGTLTVYGATAIFTCNTGYTLVGSHVRECLANGLWSGSETRCLAGHCGSPDPIVNGHISGDGFSYRDTVVYQCNPGFRLVGTSVRICLQDHKWSGQTPVCVRITCGHPGNPAHGFTNGSEFNLNDVVNFTCNTGYLLQGVSRAQCRSNGQWSSPLPTCRVVNCSDPGFVENAIRHGQQNFPESFEYGMSILYHCKKGFYLLGSSALTCMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGAVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTKSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSSSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTVISCGDPGTLANGIQFGTDFTFNKTVSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSISYSCMDGYQLSHSAILSCEGRGVWKGEIPQCLAVFCGDPGIPAEGRLSGKSFTYKSEVFFQCKSPFILVGSSRRVCQADGTWSGIQPTCIGDPAHNTCPDPGTPHFGIQNSSRGYEVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIRHACRQPETPAHADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKSKGVREVNETVTKTPVPSDVFFVNSLWKGYYEYLGKRQPATLTVDWFNATSSKVNATFSEASPVELKLTGTYKKEEAHLLLKAFQIKGQADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFKLERQDPLNPDQDSSSHYHGTSSGSVAAAILVPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMYDTNLKPTEAKAVRFDTTLNTVCTVVNOV54c, 275631102SEQ ID NO:1113898 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCAGATCTTGTGGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCCGTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGCCAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGAAATAATCCTGGATTCTGTGGTGATCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAGAGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACGTGTTTGCTCAATGGGTCATGGTCAGGACTGCAGCCGGTGTGTGAGGCCGTGTCCTGTGGCAACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGCTCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGCACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACTGCACAATTATAAGTTGTGGGGATCCAGGCACACTAGCAAATGGCATCCAGTTTGGGACCGACTTCACCTTCAACAAGACTGTGAGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGTACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGCCGTGCTGTGTCCTCAGCCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATAAGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGTCGCGGGGTGTGGAAAGGAGAGATCCCCAGTGTCTGCCTCGAGGGCNOV54c, 275631102SEQ ID NO:1114299 aaMW at 31648.2 kDProtein SequenceTRSCGHPGVPANAVLTGELFTYGAVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTKSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSSSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTVSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSISYSCMDGYQLSHSAILSCEGRGVWKGEIPSVCLEGNOV54d, CG59905-02SEQ ID NO:11153019 bpDNA SequenceORF Start: ATG at 184ORF Stop: TAA at 2614CTGCCAGGCTGTGTCCTGTGGAATCCCAGAATCCCCAGGAAACGGTTCATTACCGGGAACGAGTTCACTTTGGACAGTAAAGTGGTCTATGAATGTCATGAGGGCTTCAAGCTTGAATCCAGCCAGCAAGCAACAGCCGTGTGTCAAGAAGATGGGCTGTGGAGTAACAAGGGGAAGCCGCCCATGTGTAAGCCGGTCGCTTGCCCCAGCATTGAAGCTCAGCTCTCAGAACATGTCATCTGGAGGCTGGTTTCAGGATCCTTGAATGAGTACGGTGCTCAAGTATTGCTGAGCTGCAGTCCTGGTTACTACTTAGAAGGCTGGAGGCTCCTGCGGTGCCAGGCCAATGGGACGTGGAGCATAGGAGATGAGAGGCCAAGCTGTCGAGTTATCTCGTGTGGAAGCCTTTCCTTTCCCCCAAATGGCAACAAGATTGGAACGTTGACAGTTTATGGGGCCACAGCTATATTTACGTGCAACACCGGCTACACGCTTGTGGGGTCTCATGTCAGAGAGTGCTTGGCAAATGGGCTCTGGAGCGGCAGCGAAACTCGATGTCTGGCTGGCCACTGCGGTTCCCCAGACCCGATTGTGAACGGTCACATTAGTGGAGATGGCTTCAGTTACAGAGACACGGTGGTTTACCAGTGCAATCCTGGTTTCCGGCTTGTGGGAACTTCCGTGAGGATATGCCTGCAAGACCACAAGTGGTCTGGACAAACGCCTGTCTGTGTCGCCATCACATGTGGTCACCCTGGAAACCCTGCCCACGGATTCACTAATGGCAGTGAGTTCAACCTGAATGATGTCGTGAATTTCACCTGCAACACGGGCTATTTGCTGCAGGGCGTGTCTCGAGCCCAGTGTCGGAGCAACGGCCAGTGGAGTAGCCCTCTGCCCACGTCTCGACACGTGGGCAGAGGGCTACTCCACTGGCGGTTGCTCCGACACGGGGCTCCGAGACACGCCCTGCAGCAAATAGCCCGTGTTGCAGGTGAAATTCACGACATCATTATTTTACTTGCTGGATCTTCAGCCTTGACCTGTATGGCAAATGGCTTATGGGACCGATCCCTGCCCAAGTGTTTGGCTATATCGTGTGGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCCGTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGCCAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGTAATAATCCTGGATTCTGTGGTGATCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAGAGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACGTGTTTGCTCAATGGGTCATGGTCAGGACTGCAGTCGGTGTGTGAGGCCGTGTCCTGTGGCAACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGCTCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGCACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACTGCTTAGTTATAAGTTGTGGGGATCCAGGCACACTAGCAAATGGCATCCAGTTTGGGACCGACTTCACCTTCAACAAGACTGTGAGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGTACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGGTTTGCTGTGTCCTCAGCCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATAAGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGTCGCGGGGTGTGGAAAGGAGAGATCCCCCAGTGTCTGCCTGTGTTCTGCGGAGACCCTGGCATCCCCGCAGAGGGGCGACTTAGTGGGAAAAGTTTCACCTATAAGTCCGAAGTCTTCTTCCAGTGCAAATCTCCATTTATACTCGTGGGATCCTCCAGAAGAGTCTGCCAAGCTGACGGCACGTGGAGCGGCATACAACCCACCTGCATTGATCCTGCTCATAACACCTGCCCAGACCCTGGTACGCCACACTTTGGAATACAGAATAGCTCCAGAGGCTATGAGGTTGGAAGCACGGTTTTTTTCAGGTGCAGAAAAGGCTACCATATTCAAGGTTCCACGACTCGCACCTGCCTTGCCAATTTAACATGGAGTGGGATACAGACCGAATGTATACCTCATGCCTGCAGACAGCCAGAAACCCCGGCACACGCGGATGTGAGAGCCATCGATCTTCCTACTTTCGGCTACACCTTAGTGTACACCTGCCATCCAGGCTTTTTCCTCGCAGGGGGATCTGAGCACAGAACATGTAAAGCAGACATGAAATGGACAGGAAAGTCGCCTGTGTGTAAAAGTAAGTCATTGATCAAGGAGTGGCATACTTTTCCAAAGAGCAAGGGAAAGGATCAGCTGCCCATCAGGCTAGTGTCCACGTGTGAGACCCATAAGTAAGAGCATGAGGCTGCAGCATCGCCACCTGCCTCAGTCGTGCTTCATGTGCGTGAGCTGTGTGTGTGAACGTGTGTATCCTCAGTGTTTGATGCAATGGTGGCATAGCTAAATATATAGTACAATTAGTATGCAATTAGTACATAATAAAATGATTGCATTATTTCAAGCCAAAAGAGAAAAGCTATTTGGTTAAAGAAAAGCATTCTGAGTTAAAAGGAAAATAATACTTGGTATAGTTTCAGGAAAAGTAGTTCTTAGAGATGAAAATGAATAAAGGTCACCAAGTATCCGAGAATAGGTAAATGAAACTCTGTCTTATTTTTAAGACCACATGCTGTTTGTGGTAAAACCTCAGTTTATGTGTCCCTGACATTGATTCTGCTTTTTAAAATTACAAAAGCAANOV54d, CG59905-02SEQ ID NO:1116810 aaMW at 87208.0 kDProtein SequenceMCKPVACPSIEAQLSEHVTWRLVSGSLNEYGAQVLLSCSPGYYLEGWRLLRCQANGTWSIGDERPSCRVISCGSLSFPPNGNKIGTLTVYGATAIFTCNTGYTLVGSHVRECLANGLWSGSETRCLAGHCGSPDPIVNGHISGDGFSYRDTVVYQCNPGFRLVGTSVRICLQDHKWSGQTPVCVAITCGHPGNPAHGFTNGSEFNLNDVVNFTCNTGYLLQGVSRAQCRSNGQWSSPLPTSRHVGRGLLHWRLLRHGAPRRALQQIARVAGEIHDIIILLAGSSALTCMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGAVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTKSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQSVCEAVSCGNPGTPTNGMIVSSDGILFSSSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCLVISCGDPGTLANGIQFGTDFTFNKTVSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKGLLCPQPPPVQNGTVEGSDFRWGSSISYSCMDGYQLSHSAILSCEGRGVWKGEIPQCLPVFCGDPGIPAEGRLSGKSFTYKSEVFFQCKSPFILVGSSRRVCQADGTWSGIQPTCIDPAHNTCPDPGTPHFGIQNSSRGYEVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIPHACRQPETPAHADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKSKSLIKEWHTFPKSKGKDQLPIRLVSTCETHKNOV54e, SNP13382527 ofSEQ ID NO:11173145 bpCG59905-01, DNA SequenceORF Start: ATG at 34ORF Stop: TGA at 3046SNP Pos: 392SNP Change: T to CATTTTACTTGCTGGATCTTCAGCCTTGACCTGTATGGCAAATGGCTTATGGGACCGATCCCTGCCCAAGTGTTTGGCTATATCCTGTGGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCCGTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGCCAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGAAATAATCCTGGATTCTGTGGTGATCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAGAGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACGTGTTCGCTCAATGGGTCATGGTCAGGACTGCAGCCGGTGTGTGAGGCCGTGTCCTGTGGCAACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGCTCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGCACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACTGCACAATTATAAGTTGTGGGGATCCAGGCACACTAGCAAATGGCATCCAGTTTGGGACCGACTTCACCTTCAACAAGACTGTGAGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGTACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGCCGTGCTGTGTCCTCAGCCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATAAGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGTCGCGGGGTGTGGAAAGGAGAGATCCCCAGTGTCTGCCTGTGTTCTGCGGAGACCCTGGCATCCCCGCAGAAGGGCGACTTAGTGGGAAAAGTTTCACCTATAAGTCCGAAGTCTTCTTCCAGTGCAAATCTCCATTTATACTCGTGGGATCCTCCAGAAGAGTCTGCCAAGCTGACGGCACGTGGAGCGGCATACAACCCACCTGCATTGGTAATAATTATCATACAGCTCTGGGGATACCTGGGAGTATTTGGAGATGAGGACGCTTCATTCCGAAATTGGGTCATTTGTGATTACATAGAAAGTGTTTCCATAGACAGTTTTCTGTACAAGGTTGGAAGCACGGTTTTTTTCAGGTGCAGAAAAGGCTACCATATTCAAGGTTCCACGACTCGCACCTGCCTTGCCAATTTAACATGGAGTGGGATACAGACCGAATGTATACCTCATGCCTGCAGACAGCCAGAAACCCCGGCACACGCGGATGTGAGAGCCATCGATCTTCCTACTTTCGGCTACACCTTAGTGTACACCTGCCATCCAGGCTTTTTCCTCGCAGGGGGATCTGAGCACAGAACATGTAAAGCAGACATGAAATGGACAGGAAAGTCGCCTGTGTGTAAAATTCCTTCAGATGTCTTTTTCGTCAATTCACTGTGGAAGGGGTATTATGAATATTTAGGGAAAAGACAACCCGCCACTCTAACTGTTGACTGGTTCAATGCAACAAGCAGTAAGGTGAATGCCACCTTCAGCGAAGCCTCGCCAGTGGAGCTGAAGTTGACAGGCATTTACAAGAAGGAGGAGGCCCACTTACTCCTGAAAGCTTTTCAAATTAAAGGCCAGGCAGATATTTTTGTAAGCAAGTTCGAAAATGACAACTGGGGACTAGATGGTTATGTGTCATCTGGACTTGAAAGAGGAGGATTTACTTTTCAAGGTGACATTCATGGAAAAGACTTTGGAAAATTTAAGCTAGAAAGGCAAGGATGGGTCACAATATTCTTGAGCCTATTTCTTCATCTTAAATCTCAGTATAGAAGTTCCCAAGGTTGTTACGAGATTGAGAGGCCACATCCTTTAAACCCAGATCAAGACTCTTCCAGTCATTACCACGGCACCAGCAGTGGCTCTGTGGCGGCTGCCATTCTGGTTCCTTTCTTTGCTCTAATTTTATCAGGGTTTGCATTTTACCTCTACAAACACAGAACGAGACCAAAAGTTCAATACAATGGCTATGCTGGGCATGAAAACAGCAATGGACAAGCATCGTTTGAAAACCCCATGTATGATACAAACTTAAAACCCACAGAAGCCAAGGCTAAAACCACACACGGGCTGCTCACGTCTCTGGACTCCAAGAGAAAGATGGTTATAACCCTGGCAGAGTCTTTACCCGCCCTGCGAACCTGCCTGTTGATCTGCTTCCCACTCCTCTTTAGACTTGCTCGTGAATCTTCAGCACAGCCATTTGTTTTGAGTATTCAAACACATAAAAAGATATCTGGCAACTTCCCACTTCTTATGACTTCTCAGACACCTTGTAACGGAGCTTCCCTTGGAGGTGGACAAACTTCTCACTCACAAGAGAGACGGAGAGCAACAGAGAGAGATGGGCAGGGATTGATTCGAGGATTGGCTGACTCGATTATGGAGGGTGAGAAGTCCCAGGACAGGCCGTCTGCAAGGCGTAAACCCAGGGAAGCTGCTGGTATGGCTCGGTTCAAGTCCAAAGGCCTCAGCACCAAGGAAACCAAGGTGATAACTCTCAGTTCGGGTCAAAGTCCTGGGAGTCTGCAAGGCCTCTCATTGAGTAAGTCTCACAAGATCCGATGGTTTTATAAAGGGCAGTTCCCCTGCACAAGCTCTCTTCTCTGCCACCACGCGTTTGCTGTTCATTCACCTTTCACCATGATTGTGAGGCCTCCCAGCCATGTGGAACTGTGCAAAGAGGATGTTCAAAGGGCATCTTGGACCAGGAGGAGGAAGCCCATTGTGAGGATGGCAAAGAACAATAAATGGAAGGCCCGTGGAACCCTGAGCGGCTGCCCCATCACTGTCCTCTCCATCCTTTCCATGCTTCCCATGGTGCATGGCTCTCAGGCTCTCCAAGCACCATGAGCTCCTGGCGCGTATGGCCTGGCCCCTGAGTGTGGAGTCTGTGGACTTTAAATGTGCCTTTACTCAGCCAGCATCTCCCTGGCTGGGTGCGATGTTGNOV54e, SNP13382527 ofSEQ ID NO:11181004 aaMW at 109726.6 kDCG59905-01, ProteinSNP Pos: 120SNP Change: Leu to SerSequenceMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGAVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTKSLLRFSCEMGHQLRGSPERTCSLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSSSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTVSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSISYSCMDGYQLSHSAILSCEGRGVWKGEIPSVCLCSAETLASPQKGDLVGKVSPISPKSSSSANLHLYSWDPPEESAKLTARGAAYNPPALVTIIIQLWGYLGVFGDEDASFRNWVICDYIESVSIDSFLYKVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIPHACRQPETPAHADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKIPSDVFFVNSLWKGYYEYLGKRQPATLTVDWFNATSSKVNATFSEASPVELKLTGIYKKEEAHLLLKAFQIKGQADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFKLERQGWVTIFLSLFLHLKSQYRSSQGCYEIERPHPLNPDQDSSSHYHGTSSGSVAAAILVPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMYDTNLKPTEAKAKTTHGLLTSLDSKRKMVITLAESLPALRTCLLICFPLLFRLARESSAQPFVLSIQTHKKISGNFPLLMTSQTPCNGASLGGGQTSHSQERRRATERDGQGLIRGLADSIMEGEKSQDRPSARRKPREAAGMARFKSKGLSTKETKVITLSSGQSPGSLQGLSLSKSHKIRWFYKGQFPCTSSLLCHHAFAVHSPFTMIVRPPSHVELCKEDVQRASWTRRRKPIVRMAKNNKWKARGTLSGCPITVLSILSMLPMVHGSQALQAPNOV54f, SNP13382520 ofSEQ ID NO:11193145 bpCG59905-01, DNA SequenceORF Start: ATG at 34ORF Stop: TGA at 3046SNP Pos: 1620SNP Change: A to TATTTTACTTGCTGGATCTTCAGCCTTGACCTGTATGGCAAATGGCTTATGGGACCGATCCCTGCCCAAGTGTTTGGCTATATCCTGTGGACACCCAGGGGTCCCTGCCAACGCCGTCCTCACTGGAGAGCTGTTTACCTATGGCGCCGTCGTGCACTACTCCTGCAGAGGGAGCGAGAGCCTCATAGGCAACGACACGAGAGTGTGCCAGGAAGACAGTCACTGGAGCGGGGCACTGCCCCACTGCACAGGAAATAATCCTGGATTCTGTGGTGCTCCGGGGACCCCAGCACATGGGTCTCGGCTTGGTGATGACTTTAAGACAAAGAGTCTTCTCCGCTTCTCCTGTGAAATGGGGCACCAGCTGAGGGGCTCCCCTGAACGCACGTGTTTGCTCAATGGGTCATGGTCAGGACTGCAGCCGGTGTGTGAGGCCGTGTCCTGTGGCAACCCTGGCACACCCACCAACGGAATGATTGTCAGTAGTGATGGCATTCTGTTCTCCAGCTCGGTCATCTATGCCTGCTGGGAAGGCTACAAGACCTCAGGGCTCATGACACGGCATTGCACAGCCAATGGGACCTGGACAGGCACTGCTCCCGACTGCACAATTATAAGTTGTGGGGATCCAGGCACACTAGCAAATGGCATCCAGTTTGGGACCGACTTCACCTTCAACAAGACTGTGAGCTATCAGTGTAACCCAGGCTATGTCATGGAAGCAGTCACATCCGCCACTATTCGCTGTACCAAAGACGGCAGGTGGAATCCGAGCAAACCTGTCTGCAAAGCCGTGCTGTGTCCTCAGCCGCCGCCGGTGCAGAATGGAACAGTGGAGGGAAGTGATTTCCGCTGGGGCTCCAGCATAAGTTACAGCTGCATGGACGGTTACCAGCTCTCTCACTCCGCCATCCTCTCCTGTGAAGGTCGCGGGGTGTGGAAAGGAGAGATCCCCAGTGTCTGCCTGTGTTCTGCGGAGACCCTGGCATCCCCGCAGAAGGGCGACTTAGTGGGAAAAGTTTCACCTATAAGTCCGAAGTCTTCTTCCAGTGCAAATCTCCATTTATACTCGTGGGATCCTCCAGAAGAGTCTGCCAAGCTGACGGCACGTGGAGCGGCATACAACCCACCTGCATTGGTAATAATTATCATACAGCTCTGGGGATACCTGGGAGTATTTGGAGATGAGGACGCTTCATTCCGAAATTGGGTCATTTGTGATTACATAGAAAGTGTTTCCATAGACAGTTTTCTGTACAAGGTTGGAAGCACGGTTTTTTTCAGGTGCAGAAAAGGCTACCATATTCAAGGTTCCACGACTCGCACCTGCCTTGCCAATTTAACATGGAGTGGGATACAGACCGAATGTATACCTCATGCCTGCAGACAGCCAGAAACCCCGGCACACGCGGATGTGAGAGCCATCGATCTTCCTACTTTCGGCTACACCTTAGTGTACACCTGCCATCCAGGCTTTTTCCTCGCAGGGGGATCTGAGCACAGAACATGTAAAGCAGACATGAAATGGACAGGAAAGTCGCCTGTGTGTAAAATTCCTTCAGATGTCTTTTTCGTCAATTCACTGTGGAAGGGGTATTATGAATATTTAGGGAAAAGACATCCCGCCACTCTAACTGTTGACTGGTTCAATGCAACAAGCAGTAAGGTGAATGCCACCTTCAGCGAAGCCTCGCCAGTGGAGCTGAAGTTGACAGGCATTTACAAGAAGGAGGAGGCCCACTTACTCCTGAAAGCTTTTCAAATTAAAGGCCAGGCAGATATTTTTGTAAGCAAGTTCGAAAATGACAACTGGGGACTAGATGGTTATGTGTCATCTGGACTTGAAAGAGGAGGATTTACTTTTCAAGGTGACATTCATGGAAAAGACTTTGGAAAATTTAAGCTAGAAAGGCAAGGATGGGTCACAATATTCTTGAGCCTATTTCTTCATCTTAAATCTCAGTATAGAAGTTCCCAAGGTTGTTACGAGATTGAGAGGCCACATCCTTTAAACCCAGATCAAGACTCTTCCAGTCATTACGTTTGCATTTTACCTCTACAAACACAGAACGAGACCAAAAGTTCAATACAATGGCTATGCTGGGCATGAAAACAGCAATGGACAAGCATCGTTTGAAAACCCCATGTATGATACAAACTTAAAACCCACAGAAGCCAAGGCTAAAACCACACACGGGCTGCTCACGTCTCTGGACTCCAAGAGAAAGATGGTTATAACCCTGGCAGAGTCTTTACCCGCCCTGCGAACCTGCCTGTTGATCTGCTTCCCACTCCTCTTTAGACTTGCTCGTGAATCTTCAGCACAGCCATTTGTTTTGAGTATTCAAACACATAAAAAGATATCTGGCAACTTCCCACTTCTTATGACTTCTCAGACACCTTGTAACGGAGCTTCCCTTGGAGGTGGACAAACTTCTCACTCACAAGAGAGACGGAGAGCAACAGAGAGAGATGGGCAGGGATTGATTCGAGGATTGGCTGACTCGATTATGGAGGGTGAGAAGTCCCAGGACAGGCCGTCTGCAAGGCGTAAACCCAGGGAAGCTGCTGGTATGGCTCGGTTCAAGTCCAAAGGCCTCAGCACCAAGGAAACCAAGGTGATAACTCTCAGTTCGGGTCAAAGTCCTGGGAGTCTGCAAGGCCTCTCATTGAGTAAGTCTCACAAGATCCGATGGTTTTATAAAGGGCAGTTCCCCTGCACAAGCTCTCTTCTCTGCCACCACGCGTTTGCTGTTCATTCACCTTTCACCATGATTGTGAGGCCTCCCAGCCATGTGGAACTGTGCAAAGAGGATGTTCAAAGGGCATCTTGGACCAGGAGGAGGAAGCCCATTGTGAGGATGGCAAAGAACAATAAATGGAAGGCCCGTGGAACCCTGAGCGGCTGCCCCATCACTGTCCTCTCCATCCTTTCCATGCTTCCCATGGTGCATGGCTCTCAGGCTCTCCAAGCACCATGAGCTCCTGGCGCGTATGGCCTGGCCCCTGAGTGTGGAGTCTGTGGACTTTAAATGTGCCTTTACTCAGCCAGCATCTCCCTGGCTGGGTGCGATGTTGNOV54f, SNP13382520 ofSEQ ID NO:11201004 aaMW at 109761.8 kDCG59905-01, Protein SequenceSNP Pos: 529SNP Change: Gln to HisMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGAVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTKSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSSSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTVSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSISYSCMDGYQLSHSAILSCEGRGVWKGEIPSVCLCSAETLASPQKGDLVGKVSPISPKSSSSANLHLYSWDPPEESAKLTARGAAYNPPALVIIIIQLWGYLGVFGDEDASFRNWVICDYIESVSIDSFLYKVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIPHACRQPETPAHADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKIPSDVFFVNSLWKGYYEYLGKRHPATLTVDWFNATSSKVNATFSEASPVELKLTGIYKKEEAHLLLKAFQIKGQADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFKLERQGWVTIFLSLFLHLKSQYRSSQGCYEIERPHPLNPDQDSSSHYHGTSSGSVAAAILVPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMYDTNLKPTEAKAKTTHGLLTSLDSKRKMVITLAESLPALRTCLLICFPLLFRLARESSAQPFVLSIQTHKKISGNFPLLMTSQTPCNGASLGGGQTSHSQERRRATERDGQGLIRGLADSIMEGEKSQDRPSARRKPREAAGMARFKSKGLSTKETKVITLSSGQSPGSLQGLSLSKSHKIRWFYKGQFPCTSSLLCHHAFAVHSPFTMIVRPPSNVELCKEDVQRASWTRRRKPIVRMAKNNKWKARGTLSGCPITVLSILSMLPMVHGSQALQAP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 54B.

TABLE 54BComparison of the NOV54 protein sequences.NOV54a ------------------------------------------------------------NOV54b MTAWRRFQSLLLLLGLLVLCARLLTAAKGQNCGGLVQGPNGTIESPGFPHGYPNYANCTWNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b IIITGERNRIQLSFHTFALEENFDILSVYDGQPQQGNLKVRLSGFQLPSSIVSTGSILTLNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b WFTTDFAVSAQGFKALYEVLPSHTCGNPGEILKGVLHGTRFNIGDKIRYSCLPGYILEGHNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b AILTCIVSPGNGASWDFPAPFCHAEGACGGTLRGTSSSISSPHFPSEYENNADCTWTILANOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b EPGDTIALVFTDFQLEEGYDFLEISGTEAPSIWLTGMNLPSPVISSKNWLRLHFTSDSNHNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b RRKGFNAQFQVKKAIELKSRGVKMLPSKDGSHKNSVLSQGGVALVSHMCLDPGIPENGRRNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b AGSDFSRVGANVQFSCEDNYVLQGSKSTTCQRVTETLAAWSDHRPICRARTCGSNLRGPSNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b GVITSPNYPVQYEDNAHCVWVITTTDPDKVIKLAFEEFELERGYDTLTVGDAGKVGDTRSNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b VLYVLTGSSVPDLIVSMSNQMWLHLQSDDSIGSPGFKAVYQEIEKGGCGDPGIPAYGKRTNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b GSSFLHGDTLTFECPAAFELVGERVITCQQNNQWSGNKPSCVFSCFFNFTASSGIILSPNNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b YPEEYGNNMNCVWLTISEPGSRIHLIFNDFDVEPQFDFLAVKDNGISDITVLGTFSGNEVNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b PSQLASSGHIVRLEFQSDHSTTGRGFNITYTTFGQNECHDPGIPINGRRFGDRFLLGSSVNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b SFHCDDGFVKTQGSESITCILQDGNVVWSSTVPRCEAPCGGHLTASSGVILPPGWPGYYKNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b DSLHCEWIIEAKPGHSIKMTFDRFQTEVNYDTLEVRDGPASSSPLIGEYHGTQAPQFLISNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b TGNFMYLLFTTDNSRSSIGFLIHYESVTLESDSCLDPGIPVNGHRHGGDFGIRSTVTFSCNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b DPGYTLSDDEPLVCERNHQWNHALPSCDALCGGYIQGKSGTVLSPGFPDFYPNSLNCTWTNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b IEVSHGKGVQMIFHTFHLESSHDYLLITEDGSFSEPVARLTGSVLPHTIKAGLFGNFTAQNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b LRFISDFSISYEGFNITFSEYDLEPCDDPGVPAFSRRIGFHFGVGDSLTFSCFLGYRLEGNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b ATKLTCLGGGRRVWSAPLPRCVAECGASVKGNEGTLLSPNFPSNYDNTHECIYKIETEAGNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b KGIHLRTRSFQLFEGDTLKVYDGKDSSSRPLGTFTKNELLGLILNSTSNHLWLEFNTNGSNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b DTDQGFQLTYTSFDLVKCEDPGIPNYGYRIRDEGHFTDTVVLYSCNPGYANHGSNTLTCLNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b SGDPRVWDKPLPSCIAECGGQIHAATSGRILSPGYPAPYDNNLHCTWIIEADPGKTISLHNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b FIVFDTEMAHDILKVWDGPVDSDILLKEWSGSALPEDIHSTFNSLTLQFDSDFFISKSGFNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b SIQFSTSIAATCNDPGMPQNGTRYGDSREAGDTVTFQCDPGYQLQGQAKITCVQLNNRFFNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b WQPDPPTCIAACGGNLTGPAGVILSPNYPQPYPPGKECDWRVKVNPDFVIALIFKSFNMENOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b PSYDFLHIYEGEDSNSPLIGSYQGSQAPERIESSGNSLFLAFRSDASVGLSGFAIEFKEKNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b PREACFDPGNIMNGTRVGTDFKLGSTITYQCDSGYKILDPSSITCVIGADGKPSWDQVLPNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b SCNAPCGGQYTGSEGVVLSPNYPHNYTAGQICLYSITVPKEFVVFGQFAYFQTALNDLAENOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b LFDGTHAQARLLSSLSGSHSGETLPLATSNQILLRFSAKSGASARGFHFVYQAVPRTSDTNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b QCSSVPEPRYGRRIGSEFSAGSIVRFECNPGYLLQGSTALHCQSVPNALAQWNDTIPSCVNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b VPCSGNFTQRRGTILSPGYPEPYGNNLNCIWKIIVTEGSGIQDPSDQFCHGAELGLPFEINOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b HDGGDVTAPRLGSFSGTTVPALLNSTSNQLYLHFQSDISVAAAGFHLEYKTVGLAACQEPNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b ALPSNSIKIGDRYMVNDVLSFQCEPGYTLQGRSHISCMPGTVRRWNYPSPLCIATCGGTLNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b STLGGVILSPGFPGSYPNNLDCTWRISLPIGYGAHIQFLNFSTEANHDFLEIQNGPYHTSNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b PMIGQFSGTDLPAALLSTTHETLIHFYSDHSQNRQGFKLAYQAYELQNCPDPPPFQNGYMNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b INSDYSVGQSVSFECYPGYILIGHPVLTCQHGINRNWNYPFPRCDAPCGYNVTSQNGTIYNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b SPGFPDEYPILKDCIWLITVPPGHGVYINFTLLQTEAVNDYIAVWDGPDQNSPQLGVFSGNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b NTALETAYSSTNQVLLKFHSDFSNGGFFVLNFHAFQLKKCQPPPAVPQAEMLTEDDDFEINOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b GDFVKYQCHPGYTLVGTDILTCKLSSQLQFEGSLPTCEAQCPANEVRTGSSGVILSPGYPNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b GNYFNSQTCSWSIKVEPNYNITIFVDTFQSEKQFDALEVFDGSSGQSPLLVVLSGNHTEQNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b SNFTSRSNQLYLRWSTDHATSKKGFKIRYAAPYCSLTHPLKNGGILNRTAGAVGSKVHYFNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b CKPGYRMVGHSNATCRRNPLGMYQWDSLTPLCQAVSCGIPESPGNGSFTGNEFTLDSKVVNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ------------------------------------------------------------NOV54b YECHEGFKLESSQQATAVCQEDGLWSNKGKPPMCKPVACPSIEAQLSEHVIWRLVSGSLNNOV54c ------------------------------------------------------------NOV54d --------------------------------MCKPVACPSIEAQLSENVIWRLVSGSLNNOV54a ------------------------------------------------------------NOV54b EYGAQVLLSCSPGYYLEGWRLLRCQANGTWNIGDERPSCRVISCGSLSFPPNGNKIGTLTNOV54c ------------------------------------------------------------NOV54d EYGAQVLLSCSPGYYLEGWRLLRCQANGTWSIGDERPSCRVISCGSLSFPPNGNKIGTLTNOV54a ------------------------------------------------------------NOV54b VYGATAIFTCNTGYTLVGSHVRECLANGLWSGSETRCLAGHCGSPDPIVNGHISGDGFSYNOV54c ------------------------------------------------------------NOV54d VYGATAIFTCNTGYTLVGSHVRECLANGLWSGSETRCLAGHCGSPDPIVNGHISGDGFSYNOV54a ------------------------------------------------------------NOV54b RDTVVYQCNPGFRLVGTSVRICLQDHKWSGQTPVCVRITCGHPGNPAHGFTNGSEFNLNDNOV54c ------------------------------------------------------------NOV54d RDTVVYQCNPGFRLVGTSVRICLQDHKWSGQTPVCVAITCGHPGNPAHGFTNGSEFNLNDNOV54a ------------------------------------------------------------NOV54b VVNFTCNTGYLLQGVSPAQCRSNGQWSSPLPTCRVVNCSDPGFVENAIRHGQQNFPESFENOV54c ------------------------------------------------------------NOV54d VVNFTCNTGYLLQGVSRAQCRSNGQWSSPLPTSRHVGRG--LLHWRLLRHG-APRHALQQNOV54a -----------------------MANGLWDRSLPKCLATSCGHPGVPANAVLTGELFTYGNOV54b YGMSILYHCKKGFYLLGSSALTCMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGNOV54c -------------------------------------TRSCGHPGVPANAVLTGELFTYGNOV54d IARVAGEIHDIIILLAGSSALTCMANGLWDRSLPKCLAISCGHPGVPANAVLTGELFTYGNOV54a AVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTNOV54b AVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTNOV54c AVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTNOV54d AVVHYSCRGSESLIGNDTRVCQEDSHWSGALPHCTGNNPGFCGDPGTPAHGSRLGDDFKTNOV54a KSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSNOV54b KSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSNOV54c KSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQPVCEAVSCGNPGTPTNGMIVSSDGILFSNOV54d KSLLRFSCEMGHQLRGSPERTCLLNGSWSGLQSVCEAVSCGNPGTPTNGMIVSSDGILFSNOV54a SSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTNOV54b SSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTVISCGDPGTLANGIQFGTDFTFNKTNOV54c SSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCTIISCGDPGTLANGIQFGTDFTFNKTNOV54d SSVIYACWEGYKTSGLMTRHCTANGTWTGTAPDCLVISCGDPGTLANGIQFGTDFTFNKTNOV54a VSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSNOV54b VSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSNOV54c VSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKAVLCPQPPPVQNGTVEGSDFRWGSSNOV54d VSYQCNPGYVMEAVTSATIRCTKDGRWNPSKPVCKGLLCPQPPPVQNGTVEGSDFRWGSSNOV54a ISYSCMDGYQLSHSAILSCEGRGVWKGEIPSVCLCSAETIASPQKGDLVGKVSPISPKSSNOV54b ISYSCMDGYQLSHSAILSCEGRGVWKGEIPQCLAVFCGDPGIPAEGRLSGKSFTYKSEVFNOV54c ISYSCMDGYQLSHSAILSCEGRGVWKGEIPSVCLEG------------------------NOV54d ISYSCMDGYQLSHSAILSCEGRGVWKGEIPQCLPVFCGDPGIPAEGRLSGKSFTYKSEVFNOV54a SSANLHLYSWDPPEESAKLTARGAAYNPPALVIIIIQLWGYLGVFGDEDASFRNWVICDYNOV54b FQCKSPFILVGSSRRVCQADGTWSGIQPTCIGDPAHNTCPDPGTPHFG------------NOV54c ------------------------------------------------------------NOV54d FQCKSPFILVGSSRRVCQADGTWSGIQPTCI-DPAHNTCPDPGTPHFG------------NOV54a IESVSIDSFLYKVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIPHACRQPETPAIWV54b ---IQNSSRGYEVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIRHACRQPETPANOV54c ------------------------------------------------------------NOV54d ---IQNSSRGYEVGSTVFFRCRKGYHIQGSTTRTCLANLTWSGIQTECIPHACRQPETPANOV54a HADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKIP---------NOV54b HADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKSKGVREVNETVNOV54c ------------------------------------------------------------NOV54d HADVRAIDLPTFGYTLVYTCHPGFFLAGGSEHRTCKADMKWTGKSPVCKSK---------NOV54a ------SDVFFVNSLWKGYYEYLGKRQPATLTVDWFNATSSKVNATFSEASPVELKLTGINOV54b TKTPVPSDVFFVNSLWKGYYEYLGKRQPATLTVDWFNATSSKVNATFSEASPVELKLTGINOV54c ------------------------------------------------------------NOV54d -------------SLIKEWHTFPKSKGKDQLPIRLVSTCETHK-----------------NOV54a YKKEEAHLLLKAFQIKGQADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFNOV54b YKKEEAHLLLKAFQIKGQADIFVSKFENDNWGLDGYVSSGLERGGFTFQGDIHGKDFGKFNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a KLERQGWVTIFLSLFLHLKSQYRSSQGCYEIERPHPLNPDQDSSSHYHGTSSGSVAAAILNOV54b KLERQ-----------------------------DPLNPDQDSSSHYHGTSSGSVAAAILNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a VPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMYDTNLKPTEAKAXTTNOV54b VPFFALILSGFAFYLYKHRTRPKVQYNGYAGHENSNGQASFENPMYDTNLKPTEAKAVRFNOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a HGLLTSLDSKRKMVITLAESLPALRTCLLICFPLLFRLARESSAQPFVLSIQTHKKISGNNOV54b DTTLNTVCTVV-------------------------------------------------NOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a FPLLMTSQTPCNGASLGGGQTSHSQERPPATERDGQGLIRGLADSIMEGEKSQDRPSARRNOV54b ------------------------------------------------------------NOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a KPREAAGMARFKSKGLSTKETKVITLSSGQSPGSLQGLSLSKSHKIRWFYKGQFPCTSSLNOV54b ------------------------------------------------------------NOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a LCHHAFAVHSPFTMIVRPPSHVELCKEDVQRASWTRPRKPIVRMAKNNKWKARGTLSGCPNOV54b ------------------------------------------------------------NOV54c ------------------------------------------------------------NOV54d ------------------------------------------------------------NOV54a ITVLSILSMLPMVHGSQALQAPNOV54b ----------------------NOV54c ----------------------NOV54d ----------------------NOV54a (SEQ ID NO: 1110)NOV54b (SEQ ID NO: 1112)NOV54c (SEQ ID NO: 1114)NOV54d (SEQ ID NO: 1116)


Further analysis of the NOV54a protein yielded the following properties shown in Table 54C.

TABLE 54CProtein Sequence Properties NOV54aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 8; pos. chg 1; neg. chg 1H-region:length 3; peak value 2.05PSG score:−2.35GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −6.14possible cleavage site: between 46 and 47>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 4INTEGRALLikelihood =Transmembrane 367-383−4.57INTEGRALLikelihood =Transmembrane 677-693−7.80INTEGRALLikelihood =Transmembrane 762-778−1.91INTEGRALLikelihood =Transmembrane 979-995−0.80PERIPHERALLikelihood = 1.75 (at 148)ALOM score: −7.80 (number of TMSs: 4)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 374Charge difference: −2.0 C(−3.0)-N(−1.0)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC:discrimination of mitochondrial targeting seqR content: 1Hyd Moment(75):14.35Hyd Moment(95):11.39G content: 4D/E content: 2S/T content: 3Score: −5.37Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 55 CRG|SENUCDISC: discrimination of nuclear localization signalspat4: RRKP (4) at 861pat4: RKPR (4) at 862pat4: RRRK (5) at 958pat4: RRKP (4) at 959pat7: PSARRKP (4) at 858bipartite: RRKPIVRMAKNNKWKAR at 959content of basic residues: 10.4%NLS Score: 1.43KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs:Ribosomal protein L23 signature (PS00050): *** found ***RKMVITLAESLPALRT at 753checking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 70.6COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: nuclear30.4%: mitochondrial26.1%: endoplasmic reticulum 4.3%: cytoplasmic 4.3%: peroxisomal>> prediction for CG59905-01 is nuc (k = 23)


A search of the NOV54a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 54D.

TABLE 54DGeneseq Results for NOV54aNOV54aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE20901Human C3b/C4b complement receptor1 . . . 328312/328 (95%)0.0like protein #2, alternative version -2378 . . . 2705 313/328 (95%)Homo sapiens, 3100 aa.[WO200210199-A2, 07-FEB-2002]AAE20900Human C3b/C4b complement receptor1 . . . 328312/328 (95%)0.0like protein #1 , alternative version -2347 . . . 2674 313/328 (95%)Homo sapiens, 3069 aa.[WO200210199-A2, 07-FEB-2002]AAE20789Human C3b/C4b complement receptor1 . . . 328312/328 (95%)0.0like protein #2 - Homo sapiens, 31002378 . . . 2705 313/328 (95%)aa. [WO200210199-A2, 07-FEB-2002]AAE20787Human C3b/C4b complement receptor1 . . . 328312/328 (95%)0.0like protein #1 - Homo sapiens, 30692347 . . . 2674 313/328 (95%)aa. [WO200210199-A2, 07-FEB-2002]ABU11478Human MDDT polypeptide SEQ ID 425 -27 . . . 328 286/302 (94%)e−176Homo sapiens, 474 aa.44 . . . 345 287/302 (94%)[WO200279449-A2, 10-OCT-2002]


In a BLAST search of public sequence databases, the NOV54a protein was found to have homology to the proteins shown in the BLASTP data in Table 54E.

TABLE 54EPublic BLASTP Results for NOV54aNOV54aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueAAK73475CUB and sushi multiple domains 11 . . . 328312/328 (95%)0.0protein - Homo sapiens (Human),2844 . . . 3171 313/328 (95%)3566 aa.CAD26764Sequence 6 from Patent1 . . . 328312/328 (95%)0.0WO0210199 - Homo sapiens2378 . . . 2705 313/328 (95%)(Human), 3100 aa (fragment).CAD26763Sequence 1 from Patent1 . . . 328312/328 (95%)0.0WO0210199 - Homo sapiens2347 . . . 2674 313/328 (95%)(Human), 3069 aa.Q96RM4CUB and sushi multiple domains 11 . . . 328312/328 (95%)0.0protein - Homo sapiens (Human),2786 . . . 3113 313/328 (95%)3508 aa.Q923L3CSMD1 - Mus musculus (Mouse),1 . . . 328279/328 (85%)e−1753564 aa.2842 . . . 3169 297/328 (90%)


PFam analysis predicts that the NOV54a protein contains the domains shown in the Table 54F.

TABLE 54FDomain Analysis of NOV54aIdentities/SimilaritiesPfamNOV54afor theDomainMatch RegionMatched RegionExpect Valuesushi18 . . . 7118/63 (29%)2.9e−1143/63 (68%)sushi 79 . . . 13215/63 (24%)  4e−1141/63 (65%)sushi137 . . . 19120/64 (31%)1.4e−0841/64 (64%)sushi196 . . . 25121/65 (32%)5.2e−1446/65 (71%)sushi256 . . . 31020/64 (31%)  4e−0945/64 (70%)sushi395 . . . 44518/62 (29%)3.2e−0739/62 (63%)sushi450 . . . 50518/64 (28%)1.4e−0941/64 (64%)


Example 55

The NOV55 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 55A.

TABLE 55ANOV55 Sequence AnalysisNOV55a, CG59932-01SEQ ID NO: 11211171 bpDNA SequenceORF Start: at 2ORF Stop: TAA at 1031GGAGGCCGCAAGGCCCTTCGCCCGGGAGTGGAGGGCCCAGTCCCTACCCCTGGCAGTAGGGGGCGTTTTGAAGCTGCGGCTCTGTGAGCTGTGGCTACTGCTACTGGGTTCTAGTTTGAACGCCAGATTTTTGCCAGACGAGGAGGACGTAGACTTTATCAACGAGTACGTGAACCTCCACAATGAGCTGCGGGGCGACGTTATTCCCCGAGGGTCTAACTTGCGCTTCATGACTTGGGATGTAGCTTTATCACGGACTGCTAGAGCATGGGGAAAAAAATGTTTGTTTACGCATAATATTTATTTACAAGATGTACAAATGGTCCATCCTAAATTTTATGGTATTGGTGAAAATATGTGGGTCGGCCCTGAAAATGAATTTACTGCAAGTATTGCTATCAGAAGTTGGCATGCAGAGAAGAAAATGTACAATTTTGAAAATGGCAGTTGCTCTGGAGACTGTTCTAATTATATTCAGCTTGTTTGGGACCACTCTTACAAAGTTGGTTGTGCTGTTACTCCATGTTCAAAAATTGGACATATTATACATGCAGCAATTTTCATATGCAACTATGCGCCAGGAGGAACACTGACGAGAAGACCTTATGAACCAGGAATATTTTGTACTCGATGTGGCAGACGTGACAAATGCACAGATTTTCTATGCAGTAATGCAGATCGTGACCAAGCCACATATTACCGATTTTGGTATCCAAAATGGGAAATGCCCCGGCCAGTTGTGTGTGATCCACTGTGCACATTCATTTTATTATTGAGAATATTATGTTTTATCCTGTGTGTCATAACTGTTTTGATAGTACAGTCTCAGTTTCCAAATATCTTGTTGGAACAACAAATGATATTTACCCCTGAGGAATCTGAAGCAGGGAATGAAGAGGAGGAAAAAGAGGAAGAGAAGAAAGAGAAAGAGGAAATGGAAATGGAAATAATGGAAATGGAGGAGGAAAAAGAAGAGAGAGAGGAGGAGGAGGAGGAAACACAAAAAGAAAAGATGGAGGAAGAGGAAAAATAAGAGTAGAAAGAGGAGGAAAAAGATGTATCACCAATATAAACCAAAAGTGTAATACAAAAAAAGACAGAAAAAAAAAAAAAGTAAAACACTGAGTTTTAACAAGAAAGAAAATATGCAAACCACCATTGGAATGTTTTTNOV55a, CG59932-01SEQ ID NO: 1122343 aaMW at 40047.3kDProtein SequenceEAARPFAREWRAQSLPLAVGGVLKLRLCELWLLLLGSSLNARFLPDEEDVDFINEYVNLHNELRGDVIHAEKKMYNFENGSCSGDCSNYIQLVWDHSYKVGCAVTPCSKIGHIIHAAIFICNYAPGGTLTRRPYEPGIFCTRCGRRDKCTDFLCSNADRDQATYYRFWYPKWEMPRPVVCDPLCTFILLLRILCFILCVITVLIVQSQFPNILLEQQMIFTPEESEAGNEEEEKEEEKKEKEEMEMEIMEMEEEKEEREEEEEETQKEKMEEEEK


Further analysis of the NOV55a protein yielded the following properties shown in Table 55B.

TABLE 55BProtein Sequence Properties NOV55aSignalP analysis:Cleavage site between residues 42 and 43PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 11; pos. chg 3; neg. chg 2H-region:length 12; peak value 7.44PSG score:3.04GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.10possible cleavage site: between 41 and 42>>> Seems to have a cleavable signal peptide (1 to 41)ALOM:Klein et al's method for TM region allocationInit position for calculation: 42Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 257-273−13.43PERIPHERALLikelihood = 2.70 (at 172)ALOM score: −13.43 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 20Charge difference: −1.0 C(1.0)-N(2.0)N >= C: N-terminal side will be inside>>> membrane topology: type 1a (cytoplasmic tail 274 to 343)MITDISC:discrimination of mitochondrial targeting seqR content: 4Hyd Moment(75):9.56Hyd Moment(95):18.17G content:2D/E content: 3S/T content:1Score: −4.20Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 11.4%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: AARPnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 281checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 94.1COIL:Lupas's algorithm to detect coiled-coil regions290 E1.00291 E1.00292 S1.00293 E1.00294 A1.00295 G1.00296 N1.00297 E1.00298 E1.00299 E1.00300 E1.00301 K1.00302 E1.00303 E1.00304 E1.00305 K1.00306 K1.00307 E1.00308 K1.00309 E1.00310 E1.00311 M1.00312 E1.00313 M1.00314 E1.00315 I1.00316 M1.00317 E1.00318 M1.00319 E1.00320 E1.00321 E1.00322 K1.00323 E1.00324 E1.00325 R1.00326 E1.00327 E1.00328 E1.00329 E1.00330 E1.00331 E1.00332 T1.00333 Q1.00334 K1.00335 E1.00336 K1.00337 M1.00338 E1.00339 E1.00340 E1.00341 E1.00342 K1.00total: 53 residuesFinal Results (k = 9/23):55.6%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: extracellular, including cell wall>> prediction for CG59932-01 is end (k = 9)


A search of the NOV55a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 55C.

TABLE 55CGeneseq Results for NOV55aNOV55aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABP69108Human polypeptide SEQ ID NO 1155 - 1 . . . 223220/223(98%) e−134Homo sapiens, 253 aa. [WO200270539- 2 . . . 224222/223(98%)A2, 12-SEP-2002]AAB64953Human secreted protein sequence45 . . . 222177/178(99%) e−109encoded by gene 12 SEQ ID NO: 131 - 1 . . . 178177/178(99%)Homo sapiens, 178 aa. [WO200076530-A1, 21-DEC-2000]AAB43408Human cancer associated protein38 . . . 282102/250(40%)6e−51sequence SEQ ID NO: 853 - Homo55 . . . 300143/250(56%)sapiens, 302 aa. [WO200055350-A1,21-SEP-2000]ABB53121Human ORF27 protein - Homo sapiens,94 . . . 17885/85(100%)2e−4885 aa. [WO200177155-A2, 18-OCT-2001]1 . . . 8585/85(100%)AAE18962Mouse testes-specific, vespid and37 . . . 28296/247(38%)1e−46pathogenic protein (RTVP) - Mus sp,18 . . . 253138/247(55%)255 aa. [WO200206344-A2, 24-JAN-2002]


In a BLAST search of public sequence databases, the NOV55a protein was found to have homology to the proteins shown in the BLASTP data in Table 55D.

TABLE 55DPublic BLASTP Results for NOV55aIdentities/ProteinNOV55aSimilaritiesAccessionResidues/for theExpectNumberProtein/Organism/LengthMatch ResiduesMatched PortionValueQ8NA43Hypothetical protein FLJ35856 -1 . . . 228228/228 (100%)e−139Homo sapiens (Human), 229 aa.2 . . . 229228/228 (100%)Q8N6N0Similar to RIKEN cDNA 4921508O111 . . . 223223/223 (100%)e−135gene - Homo sapiens (Human), 2532 . . . 224223/223 (100%)aa.Q9CQ354921508O11 Rik protein - Mus1 . . . 343209/345 (60%)e−117musculus (Mouse), 332 aa.2 . . . 331252/345 (72%)P48060Glioma pathogenesis-related protein38 . . . 282 103/250 (41%)1e−51 (GliPR) (RTVP-1 protein) - Homo19 . . . 264 144/250 (57%)sapiens (Human), 266 aa.JC5308testis-specific, vespid, and38 . . . 282 103/250 (41%)1e−51 pathogenesis-related protein 119 . . . 264 144/250 (57%)precursor - human, 266 aa.


PFam analysis predicts that the NOV55a protein contains the domains shown in the Table 55E.

TABLE 55EDomain Analysis of NOV55aIdentities/PfamNOV55aSimilarities forExpectDomainMatch Regionthe Matched RegionValueSCP57 . . . 19042/167 (25%)3.1e−3395/167 (57%)


Example 56

The NOV56 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 56A.

TABLE 56ANOV56 Sequence AnalysisNOV56a, CG92715-01SEQ ID NO: 11232800 bpDNA SequenceORF Start: ATG at 26ORF Stop: end of sequenceTAGACGCGGAGCCCAAGGAGGTAAAATGCACACTTGCTGCCCCCCAGTAACTTTGGAACAGGACCTTCACAGAAAAATGCATAGCTGGATGCTGCAGACTCTAGCGTTTGCTGTAACATCTCTCGTCCTTTCGTGTGCAGAAACCATCGATTATTACGGGGAAATCTGTGACAATGCATGTCCTTGTGAGGAAAAGGACGGCATTTTAACTGTGAGCTGTGAAAACCGGGGGATCATCAGTCTCTCTGAAATTAGCCCTCCCCGTTTCCCAATCTACCACCTCTTGTTGTCCGGAAACCTTTTGAACCGTCTCTATCCCAATGAGTTTGTCAATTACACTGGGGCTTCAATTTTGCATCTAGGTAGCAATGTTATCCAGGACATTGAGACCGGGGCTTTCCATGGGCTACGGGGTTTGAGGAGATTGCATCTAAACAATAATAAACTGGAACTTCTGCGAGATGATACCTTCCTTGGCTTGGAGAACCTGGAGTACCTACAGGTCGATTACAACTACATCAGCGTCATTGAACCCAATGCTTTTGGGAAACTGCATTTGTTGCAGGTGCTTATCCTCAATGACAATCTTTTGTCCAGTTTACCCAACAATCTTTTCCGTTTTGTGCCCTTAACGCACTTGGACCTCCGGGGGAACCGGCTGAAACTTCTGCCCTACGTGGGGCTCTTGCAGCACATGGATAAAGTTGTGGAGCTACAGCTGGAGGAAAACCCTTGGAATTGTTCTTGTGAGCTGATCTCTCTAAAGGATTGGTTGGACAGCATCTCCTATTCAGCCCTGGTGGGGGATGTAGTTTGTGAGACCCCCTTCCGCTTACACGGAAGGGACTTGGACGAGGTATCCAAGCAGGAACTTTGCCCAAGGAGACTTATTTCTGACTACGAGATGAGGCCGCAGACGCCTTTGAGCACCACGGGGTATTTACACACCACCCCGGCGTCAGTGAATTCTGTGGCCACTTCTTCCTCTGCTGTTTACAAACCCCCTTTGAAGCCCCCTAAGGGGACTCGCCAACCCAACAAGCCCAGGGTGCGCCCCACCTCTCGGCAGCCCTCTAAGGACTTGGGCTACAGCAACTATGGCCCCAGCATCGCCTATCAGACCAAATCCCCGGTGCCTTTGGAGTGTCCCACCGCGTGCTCTTGCAACCTGCAGATCTCTGATCTGGGCCTCAACGTAAACTGCCAGGAGCGAAAGATCGAGAGCATCGCTGAACTGCAGCCCAAGCCCTACAATCCCAAGAAAATGTATCTGACAGAGAACTACATCGCTGTCGTGCGCAGGACAGACTTCCTGGAGGCCACGGGGCTGGACCTCCTGCACCTGGGGAATAACCGCATCTCGATGATCCAGGACCGCGCTTTCGGGGATCTCACCAACCTGAGGCGCCTCTACCTGAATGGCAACAGGATCGAGAGGCTGAGCCCGGAGTTATTCTATGGCCTGCAGAGCCTGCAGTATCTCTTCCTCCAGTACAATCTCATCCGCGAGATTCAGTCTGGAACTTTTGACCCGGTCCCAAACCTCCAGCTGCTATTCTTGAATAACAACCTCCTGCAGGCCATGCCCTCAGGCGTCTTCTCTGGCTTGACCCTCCTCAGGCTAAACCTGAGGAGTAACCACTTCACCTCCTTGCCAGTGAGTGGAGTTTTGGACCAGCTGAAGTCACTCATCCAAATCGACCTGCATGACAATCCTTGGGATTGTACCTGTGACATTGTGGGCATGAAGCTGTGGGTGGAGCAGCTCAAAGTGGGCGTCCTAGTGGACGAGGTGATCTGTAAGGCGCCCAAAAAATTCGCTGAGACCGACATGCGCTCCATTAAGTCGGAGCTGCTGTGCCCTGACTATTCAGATGTAGTAGTTTCCACGCCCACACCCTCCTCTATCCAGGTCCCTGCGAGGACCAGCGCCGTGACTCCTGCGGTCCGGTTGAATAGCACCGGGGCCCCCGCGAGCTTGGGCGCAGGCGGAGGGGCGTCGTCGGTGCCCTTGTCTGTGTTAATTCTCAGCCTCCTGCTGGTTTTCATCATGTCCGTCTTCGTGGCCGCCGGGCTCTTCGTGCTGGTCATGAAGCGCAGGAAGAAGAACCAGAGCGACCACACCAGCACCAACAACTCCGACGTGAGCTCCTTTAACATGCAGTACAGCGTGTACGGCGGCGGCGGCGGCACGGGCGGCCACCCACACGCGCACGTGCATCACCGCGGGCCCGCGCTGCCCAAGGTGAAGACGCCCGCGGGCCACGTGTATGAATACATCCCCCACCCACTGGGCCACATGTGCAAAAACCCCATCTACCGCTCCCGAGAGGGCAACTCCGTAGAGGATTACAAAGACCTGCACGAGCTCAAGGTCACCTACAGCAGCAACCACCACCTGCAGCAGCAGCAGCAGCCGCCGCCGCCACCGCAGCAGCCACAGCAGCAGCCCCCGCCGCAGCTGCAGCTGCAGCCTGGGGAGGAGGAGAGGCGGGAAAGCCACCACTTGCGGAGCCCCGCCTACAGCGTCAGCACCATCGAGCCCCGGGAGGACCTGCTGTCGCCGGTGCAGGACGCCGACCGCTTTTACAGGGGCATTTTAGAACCAGACAAACACTGCTCCACCACCCCCGCCGGCAATAGCCTCCCGGAATATCCCAAATTCCCGTGCAGCCCCGCTGCTTACACTTTCTCCCCCAACTATGACCTGAGACGCCCCCATCAGTATTTGCACCCGGGGGCAGGGGACAGCAGGCTACGGGAACCGGTGCTCTACAGCCCCCCGAGTGCTGTCTTTGTANOV56a, CG92715-01SEQ ID NO: 1124925 aaMW at 103516.1kDProtein SequenceMHTCCPPVTLEQDLHRKMHSWMLQTLAFAVTSLVLSCAETIDYYGEICDNACPCEEKDGTLTVSCENRGIISLSEISPPRFPIYHLLLSGNLLNRLYPNEFVNYTGASILHLGSNVIQDIETGAFHGLRGLRRLHLNNNKLELLRDDTFLGLENLEYLQVDYNYISVIEPNAFGKLHLLQVLILNDNLLSSLPNNLFRFVPLTHLDLRGNRLKLLPYVGLLQHMDKVVELQLEENPWNCSCELISLKDWLDSISYSALVGDVVCETPFRLHGRDLDEVSKQELCPRRLISDYEMRPQTPLSTTGYLHTTPASVNSVATSSSAVYKPPLKPPKGTRQPNKPRVRPTSRQPSKDLGYSNYGPSIAYQTKSPvPLECPTACSCNLQISDLGLNVNCQERKIESIAELQPKPYNPKKMYLTENYIAVVRRTDFLEATGLDLLHLGNNRISMIQDRAFGDLTNLRRLYLNGNRIERLSPELFYGLQSLQYLFLQYNLIREIQSGTFDPVPNLQLLFLNNNLLQAMPSGVFSGLTLLRLNLRSNHFTSLPVSGVLDQLKSLIQIDLHDNPWDCTCDIVGMKLWVEQLKVGVLVDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTGAPASLGAGGGASSVPLSVLILSLLLVFIMSVFVAAGLFVLVMKRRKKNQSDHTSTNNSDVSSFNMQYSVYGGGGGTGGHPHAHVHHRGPALPKVKTPAGHVYEYIPHPLGHMCKNPIYRSREGNSVEDYKDLHELKVTYSSNHHLQQQQQPPPPPQQPQQQPPPQLQLQPGEEERRESHHLRSPAYSVSTIEPREDLLSPVQDADRFYRGILEPDKHCSTTPAGNSLPEYPKFPCSPAAYTFSPNYDLRRPHQYLHPGAGDSRLREPVLYSPPSAVFVNOV56b, 248576233SEQ ID NO: 11251872 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCATCGATTATTACGGGGAAATCTGTGACAATGCATGTCCTTGTGAGGAAAAGGACGGCATTTTAACTGTGAGCTGTGAAAACCGGGGGATCATCAGTCTCTCTGAAATTAGCCCTCCCCGTTTCCCAATCTACCACCTCTTGTTGTCCGGAAACCTTTTGAACCGTCTCTATCCCAATGAGTTTGTCAATTACACTGGGGCTTCAATTTTGCATCTAGGTAGCAATGTTATCCAGGACATTGAGACCGGGGCTTTCCATGGGCTACGGGGTTTGAGGAGATTGCATCTAAACAATAATAAACTGGAACTTCTGCGAGATGATACCTTCCTTGGCTTGGAGAACCCGGAGTACCTACAGGTCGATTACAACTACATCAGCGTCATTGAACCCAATGCTTTTGGGAAACTGCATTTGTTGCAGGTGCTTATCCTCAATGACAATCTTTTGTCCAGTTTACCCAACAATCTTTTCCGTTTTGTGCCCTTAACGCACTTGGACCTCCGGGGGAACCGGCTGAAACTTCTGCCCTACGTGGGGCTCTTGCAGCACATGGATAAAGTTGTGGAGCTACAGCTGGAGGAAAACCCTTGGAATTGTTCTTGTGAGCTGATCTCTCTAAAGGATTGGTTGGACAGCATCTCCTATTCAGCCCTGGTGGGGGATGTAGTTTGTGAGACCCCCTTCCGCTTACACGGAAGGGACTTGGACGAGGTATCCAAGCAGGAACTTTGCCCAAGGAGACTTATTTCTGACTACGAGATGAGGCCGCAGACGCCTTTGAGCACCACGGGGTATTTACACACCACCCCGGCGTCAGTGAATTCTGTGGCCACTTCTTCCTCTGCTGTTTACAAACCCCCTTTGAAGCCCCCTAAGGGGACTCGCCAACCCAACAAGCCCAGGGTGCGCCCCACCTCTCGGCAGCCCTCTAAGGACTTGGGCTACAGCAACTATGGCCCCAGCATCGCCTATCAGACCAAATCCCCGGTGCCTTTGGAGTGTCCCACCGCGTGCTCTTGCAACCTGCAGATCTCTGATCTGGGCCTCAACGTAAACTGCCAGGAGCGAAAGATCGAGAGCATCGCTGAACTGCAGCCCAAGCCCTACAATCCCAAGAAAATGTATCTGACAGAGAACTACATCGCTGTCGTGCGCAGGACAGACTTCCTGGAGGCCACGGGGCTGGACCTCCTGCACCTGGGGAATAACCGCATCTCGATGATCCAGGACCGCGCTTTCGGGGATCTCACCAACCTGAGGCGCCTCTACCTGAATGGCAACAGGATCGAGAGGCTGAGCCCGGAGTTATTCTATGGCCTGCAGAGCCTGCAGTATCTCTTCCTCCAGTACAATCTCATCCGCGAGATTCAGTCTGGAACTTTTGACCCGGTCCCAAACCTCCAGCTGCTATTCTTGAATAACAACCTCCTGCAGGCCATGCCCTCAGGCGTCTTCTCTGGCTTGACCCTCCTCAGGCTAAACCTGAGGAGTAACCACTTCACCTCCTTGCCAGTGAGTGGAGTTTTGGACCAGCTGAAGTCACTCATCCAAATCGACCTGCATGACAATCCTTGGGATTGTACCTGTGACATTGTGGGCATGAAGCTGTGGGTGGAGCAGCTCAAAGTGGGCGTCCTAGTGGACGAGGTGATCTGTAAGGCGCCCAAAAAATTCGCTGAGACCGACATGCGCTCCATTAAGTCGGAGCTGCTGTGCCCTGACTATTCAGATGTAGTAGTTTCCACGCCCACACCCTCCTCTATCCAGGTCCCTGCGAGGACCAGCGCCGTGACTCCTGCGGTCCGGTTGAATAGCACCGGGGCCCCCGCGAGCTTGGGCGCAGGCGGAGGGGCGTCGTCGCTCGAGNOV56b, 248576233SEQ ID NO: 1126624 aaMW at 69694.9kDProtein SequenceGSIDYYGEICDNACPCEEKDGILTVSCENRGIISLSEISPPRFPIYHLLLSGNLLNRLYPNEFVNYTGASILHLGSNVIQDIETGAFHGLRGLRRLHLNNNKLELLRDDTFLGLENPEYLQVDYNYISVIEPNAFGKLHLLQVLILNDNLLSSLPNNLFRFVPLTHLDLRGNRLKLLPYVGLLQHMDKVVELQLEENPWNCSCELISLKDWLDSISYSALVGDVVCETPFRLHGRDLDEVSKQELCPRRLISDYEMRPQTPLSTTGYLHTTPASVNSVATSSSAVYKPPLKPPKGTRQPNKPRVRPTSRQPSKDLGYSNYGPSIAYQTKSPVPLECPTACSCNLQISDLGLNVNCQERKIESIAELQPKPYNPKKMYLTENYIAVVRRTDFLEATGLDLLHLGNNRISMIQDRAFGDLTNLRPLYLNGNRIERLSPELFYGLQSLQYLFLQYNLIREIQSGTFDPVPNLQLLFLNNNLLQAMPSGVFSGLTLLRLNLRSNHFTSLPVSGVLDQLKSLIQIDLHDNPWDCTCDIVGMKLWVEQLKVGVLVDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTGAPASLGAGGGASSLENOV56c, CG92715-02SEQ ID NO: 11274500 bpDNA SequenceORF Start: ATG at 178ORF Stop: TAA at 3094CGGAACCCGCGGTCGCCACCGCGGCGGCGGCCCCAGGCTGGAGGCGTCCGGGCGCCTCTTTCCTCCAGCCTCTGGGACTGCGCTGCTCGCAGTCTCCTCGCCCTGCCTGGGCTTGAGAAACCTAGTGCATACCCCAAAGAGGGTTTTTGTGTATGTGTGTGTTTTTAAAGGGTGGCTATGATGACTGGGCCTTGGAGACGCGGAGACCAAGGAGGTAAAATGCACACTTGCTGCCCCCCAGTAACTTTGGAACAGGACCTTCACAGAAAAATGCATAGCTGGATGCTGCAGACTCTAGCGTTTGCTGTAACATCTCTCGTCCTTTCGTGTGCAGAAACCATCGATTATTACGGGGAAATCTGTGACAATGCATGTCCTTGTGAGGAAAAGGACGGCATTTTAACTGTGAGCTGTGAAAACCGGGGGATCATCAGTCTCTCTGAAATTAGCCCTCCCCGTTTCCCAATCTACCACCTCTTGTTGTCCGGAAACCTTTTGAACCGTCTCTATCCCAATGAGTTTGTCAATTACACTGGGGCTTCAATTTTGCATCTAGGTAGCAATGTTATCCAGGACATTGAGACCGGGGCTTTCCATGGGCTACGGGGTTTGAGGAGATTGCATCTAAACAATAATAAACTGGAACTTCTGCGAGATGATACCTTCCTTGGCTTGGAGAACCTGGAGTACCTACAGGTCGATTACAACTACATCAGCGTCATTGAACCCAATGCTTTTGGGAAACTGCATTTGTTGCAGGTGCTTATCCTCAATGACAATCTTTTGTCCAGTTTACCCAACAATCTTTTCCGTTTTGTGCCCTTAACGCACTTGGACCTCCGGGGGAACCGGCTGAAACTTCTGCCCTACGTGGGGCTCTTGCAGCACATGGATAAAGTTGTGGAGCTACAGCTGGAGGAAAACCCTTGGAATTGTTCTTGTGAGCTGATCTCTCTAAAGGATTGGTTGGACAGCATCTCCTATTCAGCCCTGGTGGGGGATGTAGTTTGTGAGACCCCCTTCCGCTTACACGGAAGGGACTTGGACGAGGTATCCAAGCAGGAACTTTGCCCAAGGAGACTTATTTCTGACTACGAGATGAGGCCGCAGACGCCTTTGAGCACCACGGGGTATTTACACACCACCCCGGCGTCAGTGAATTCTGTGGCCACTTCTTCCTCTGCTGTTTACAAACCCCCTTTGAAGCCCCCTAAGGGGACTCGCCAACCCAACAAGCCCAGGGTGCGCCCCACCTCTCGGCAGCCCTCTAAGGACTTGGGCTACAGCAACTATGGCCCCAGCATCGCCTATCAGACCAAATCCCCGGTGCCTTTGGAGTGTCCCACCGCGTGCTCTTGCAACCTGCAGATCTCTGATCTGGGCCTCAACGTAAACTGCCAGGAGCGAAAGATCGAGAGCATCGCTGAACTGCAGCCCAAGCCCTACAATCCCAAGAAAATGTATCTGACAGAGAACTACATCGCTGTCGTGCGCAGGACAGACTTCCTGGAGGCCACGGGGCTGGACCTCCTGCACCTGGGGAATAACCGCATCTCGATGATCCAGGACCGCGCTTTCGGGGATCTCACCAACCTGAGGCGCCTCTACCTGAATGGCAACAGGATCGAGAGGCTGAGCCCGGAGTTATTCTATGGCCTGCAGAGCCTGCAGTATCTCTTCCTCCAGTACAATCTCATCCGCGAGATTCAGTCTGGAACTTTTGACCCGGTCCCAAACCTCCAGCTGCTATTCTTGAATAACAACCTCCTGCAGGCCATGCCCTCAGGCGTCTTCTCTGGCTTGACCCTCCTCAGGCTAAACCTGAGGAGTAACCACTTCACCTCCTTGCCAGTGAGTGGAGTTTTGGACCAGCTGAAGTCACTCATCCAAATCGACCTGCATGACAATCCTTGGGATTGTACCTGTGACATTGTGGGCATGAAGCTGTGGGTGGAGCAGCTCAAAGTGGGCGTCCTAGTGGACGAGGTGATCTGTAAGGCGCCCAAAAAATTCGCTGAGACCGACATGCGCTCCATTAAGTCGGAGCTGCTGTGCCCTGACTATTCAGATGTAGTAGTTTCCACGCCCACACCCTCCTCTATCCAGGTCCCTGCGAGGACCAGCGCCGTGACTCCTGCGGTCCGGTTGAATAGCACCGGGGCCCCCGCGAGCTTGGGCGCAGGCGGAGGGGCGTCGTCGGTGCCCTTGTCTGTGTTAATTCTCAGCCTCCTGCTGGTTTTCATCATGTCCGTCTTCGTGGCCGCCGGGCTCTTCGTGCTGGTCATGAAGCGCAGGAAGAAGAACCAGAGCGACCACACCAGCACCAACAACTCCGACGTGAGCTCCTTTAACATGCAGTACAGCGTGTACGGCGGCGGCGGCGGCACGGGCGGCCACCCACACGCGCACGTGCATCACCGCGGGCCCGCGCTGCCCAAGGTGAAGACGCCCGCGGGCCACGTGTATGAATACATCCCCCACCCACTGGGCCACATGTGCAAAAACCCCATCTACCGCTCCCGAGAGGGCAACTCCGTAGAGGATTACAAAGACCTGCACGAGCTCAAGGTCACCTACAGCAGCAACCACCACCTGCAGCAGCAGCAGCAGCCGCCGCCGCCACCGCAGCAGCCACAGCAGCAGCCCCCGCCGCAGCTGCAGCTGCAGCCCGGGGAGGAGGAGAGGCGGGAAAGCCACCACTTGCGGAGCCCCGCCTACAGCGTCAGCACCATCGAGCCCCGGGAGGACCTGCTGTCGCCGGTGCAGGACGCCGACCGCTTTTACAGGGGCATTTTAGAACCAGACAAACACTGCTCCACCACCCCCGCCGGCAATAGCCTCCCGGAATATCCCAAATTCCCGTGCAGCCCCGCTGCTTACACTTTCTCCCCCAACTATGACCTGAGACGCCCCCATCAGTATTTGCACCCGGGGGCAGGGGACAGCAGGCTACGGGAACCGGTGCTCTACAGCCCCCCGAGTGCTGTCTTTGTAGAACCCAACCGGAACGAATATCTGGAGTTAAAAGCAAAACTAAACGTTGAGCCGGACTACCTCGAAGTGCTGGAAAAACAGACCACGTTTAGCCAGTTCTAAAAGCAAAGAAACTCTCTTGGAGCTTTTGCATTTAAAACAAACAAGCAAGCAGACACACACAGTGAACACATTTGATTAATTGTGTTGTTTCAACGTTTAGGGTGAAGTGCCTTGGCACGGGATTTCTCAGCTTCGGTGGAAGATACGAAAAGGGTGTGCAATTTCCTTTAAAATTTACACGTGGGAAACATTTGTGTAAACTGGGCACATCACTTTCTCTTCTTGCGTGTGGGGCAGGTGTGGAGAAGGGCTTTAAGGAGGCCAATTTGCTGCGCGGGTGACCTGTGAAAGGTCACAGTCATTTTTGTAGTGGTTGGAAGTGCTAAGAATGGTGGATGATGGCAGAGCATAGATTCTACTCTTCCTCTTTAGCTTCCTCCCCATCCAACGAACCCTGCCCAACACTCTAAATATCCACCAGATAAGACATGGAATGAGGTCTAAATGACACAAAGTGAAGAAATCAACACAACACAAACTTTACAGCTAACAACAAATGATCAACAAAAACCGAACCAACAAGACAACCATCGAACCTCACCACTCCACACTCACAACAACTCATATCAAGACAACAACACAATGACGTTAAAGGAAACGAAATCAATGCAAAAATAGACATTTGACAATACAAAAAAACAAGAACCGTGATCACACTACAACCGAAGCAACCATAGATGTGAGAAAAAACAACAAACAAAACACCGAGCTATATGATCCATAATTGATTAGTCAAAATAACTTATTGATGAAATATACAAATATTTTATTGTAGCACCTATTTTTATATGCACATTTAGCATTCCTCTTTCCTTCACTATTTAGCCTATGATTTTGCAGAGGTGTCACACTGTATTAGGATCTGCATTTCTAAAACTGACGTGGTATCAGGAAGGCATTTTCAATCATTCAAAATGTGGAGAATTTAATGGCTAAATCTTTAAAAGCCAATGCAACCCACCCAATTGAATCTGCATTTTCTTTTAAGAAAACAGAGCTGATTGTATCCCAATGTATTTTAAAAAATAGGGCAATTGATTGGGCCATTCCGAGAGAATTGTTTGCAAGTTTTGGGTTTTATTAGAAAATATTTGAAAGTATTTTTATTAATGAACCAAAATGACATGTTCATTTGACTACTATTGTAGCCGATTTTCGATTGTTTAACCAAACCCAGTTGCATTTGTACAGATCCACGTGTACTGGCACCTCAGAAGACCAAATCATGGACTGTACAAGTCTCTATACAATGTCTTTATCCCTGTGGGCAGCAAGCAATGATGATAATGACAAACAGGATATCTGTAAGATGGGGCTACTGTTGTTACAGTCTCATATGTATCCCAGCACATGTAATTTTTTAAATAGTTTCTGAATAAACACTTGATAACTATGTCAAAAAAANOV56c, CG92715-02SEQ ID NO: 1128972 aaMW at 109043.3kDProtein SequenceMMTGPWRRGDQGGKMHTCCPPVTLEQDLHRKMHSWMLQTLAFAVTSLVLSCAETIDYYGEICDNACPCEEKDGILTVSCENRGIISLSEISPPRFPIYHLLLSGNLLNRLYPNEFVNYTGASILHLGSNVIQDIETGAFHGLRGLRRLHLNNNKLELLRDDTFLGLENLEYLQVDYNYISVIEPNAFGKLHLLQVLILNDNLLSSLPNNLFRFVPLTHLDLRGNRLKLLPYVGLLQHNDKVVELQLEENPWNCSCELISLKDWLDSISYSALVGDVVCETPFRLHGRDLDEVSKQELCPRRLISDYEMRPQTPLSTTGYLHTTPASVNSVATSSSAVYKPPLKPPKGTRQPNKPRVRPTSRQPSKDLGYSNYGPSIAYQTKSPVPLECPTACSCNLQISDLGLNVNCQERKIESIAELQPKPYNPKKNYLTENYIAVVRRTDFLEATGLDLLHLGNNRTSMIQDRAFGDLTNLRRLYLNGNRIERLSPELFYGLQSLQYLFLQYNLIREIQSGTFDPVPNLQLLFLNNNLLQAMPSGVFSGLTLLRLNLRSNHFTSLPVSGVLDQLKSLIQIDLHDNPWDCTCDIVGMKLWVEQLKVGVLVDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTGAPASLGAGGGASSVPLSVLILSLLLVFIMSVFVAAGLFVLVMKRRKKNQSDHTSTNNSDVSSFNMQYSVYGGGGGTGGHPHAHVHHRGPALPKVKTPAGHVYEYIPHPLGHMCKNPIYRSREGNSVEDYKDLHELKVTYSSNHHLQQQQQPPPPPQQPQQQPPPQLQLQPGEEERRESHHLRSPAYSVSTIEPREDLLSPVQDADRFYRGILEPDKHCSTTPAGNSLPEYPKFPCSPAAYTFSPNYDLRRPHQYLHPGAGDSRLREPVLYSPPSAVFVEPNRNEYLELKAKLNVEPDYLEVLEKQTTFSQFNOV56d, CG92715-03SEQ ID NO: 11291872 bpDNA SequenceORF Start: at 7ORF Stop: at 1867GGATCCATCGATTATTACGGGGAAATCTGTGACAATGCATGTCCTTGTGAGGAAAAGGACGGCATTTTAACTGTGAGCTGTGAAAACCGGGGGATCATCAGTCTCTCTGAAATTAGCCCTCCCCGTTTCCCAATCTACCACCTCTTGTTGTCCGGAAACCTTTTGAACCGTCTCTATCCCAATGAGTTTGTCAATTACACTGGGGCTTCAATTTTGCATCTAGGTAGCAATGTTATCCAGGACATTGAGACCGGGGCTTTCCATGGGCTACGGGGTTTGAGGAGATTGCATCTAAACAATAATAAACTGGAACTTCTGCGAGATGATACCTTCCTTGGCTTGGAGAACCTGGAGTACCTACAGGTCGATTACAACTACATCAGCGTCATTGAACCCAATGCTTTTGGGAAACTGCATTTGTTGCAGGTGCTTATCCTCAATGACAATCTTTTGTCCAGTTTACCCAACAATCTTTTCCGTTTTGTGCCCTTAACGCACTTGGACCTCCGGGGGAACCGGCTGAAACTTCTGCCCTACGTGGGGCTCTTGCAGCACATGGATAAAGTTGTGGAGCTACAGCTGGAGGAAAACCCTTGGAATTGTTCTTGTGAGCTGATCTCTCTAAAGGATTGGTTGGACAGCATCTCCTATTCAGCCCTGGTGGGGGATGTAGTTTGTGAGACCCCCTTCCGCTTACACGGAAGGGACTTGGACGAGGTATCCAAGCAGGAACTTTGCCCAAGGAGACTTATTTCTGACTACGAGATGAGGCCGCAGACGCCTTTGAGCACCACGGGGTATTTACACACCACCCCGGCGTCAGTGAATTCTGTGGCCACTTCTTCCTCTGCTGTTTACAAACCCCCTTTGAAGCCCCCTAAGGGGACTCGCCAACCCAACAAGCCCAGGGTGCGCCCCACCTCTCGGCAGCCCTCTAAGGACTTGGGCTACAGCAACTATGGCCCCAGCATCGCCTATCAGACCAAATCCCCGGTGCCTTTGGAGTGTCCCACCGCGTGCTCTTGCAACCTGCAGATCTCTGATCTGGGCCTCAACGTAAACTGCCAGGAGCGAAAGATCGAGAGCATCGCTGAACTGCAGCCCAAGCCCTACAATCCCAAGAAAATGTATCTGACAGAGAACTACATCGCTGTCGTGCGCAGGACAGACTTCCTGGAGGCCACGGGGCTGGACCTCCTGCACCTGGGGAATAACCGCATCTCGATGATCCAGGACCGCGCTTTCGGGGATCTCACCAACCTGAGGCGCCTCTACCTGAATGGCAACAGGATCGAGAGGCTGAGCCCGGAGTTATTCTATGGCCTGCAGAGCCTGCAGTATCTCTTCCTCCAGTACAATCTCATCCGCGAGATTCAGTCTGGAACTTTTGACCCGGTCCCAAACCTCCAGCTGCTATTCTTGAATAACAACCTCCTGCAGGCCATGCCCTCAGGCGTCTTCTCTGGCTTGACCCTCCTCAGGCTAAACCTGAGGAGTAACCACTTCACCTCCTTGCCAGTGAGTGGAGTTTTGGACCAGCTGAAGTCACTCATCCAAATCGACCTGCATGACAATCCTTGGGATTGTACCTGTGACATTGTGGGCATGAAGCTGTGGGTGGAGCAGCTCAAAGTGGGCGTCCTAGTGGACGAGGTGATCTGTAAGGCGCCCAAAAAATTCGCTGAGACCGACATGCGCTCCATTAAGTCGGAGCTGCTGTGCCCTGACTATTCAGATGTAGTAGTTTCCACGCCCACACCCTCCTCTATCCAGGTCCCTGCGAGGACCAGCGCCGTGACTCCTGCGGTCCGGTTGAATAGCACCGGGGCCCCCGCGAGCTTGGGCGCAGGCGGAGGGGCGTCGTCGCTCGAGNOV56d, CG92715-03SEQ ID NO: 1130620 aaMW at 69324.6kDProtein SequenceIDYYGEICDNACPCEEKDGILTVSCENRGIISLSEISPPRFPIYHLLLSGNLLNRLYPNEFVNYTGASILHLGSNVIQDIETGAFHGLRGLRRLHLNNNKLELLRDDTFLGLENLEYLQVDYNYISVIEPNAFGKLHLLQVLILNDNLLSSLPNNLFRFVPLTHLDLRGNRLKLLPYVGLLQHMDKVVELQLEENPWNCSCELISLKDWLDSISYSALVGDVVCETPFRLHGRDLDEVSKQELCPRRLISDYEMRPQTPLSTTGYLHTTPASVNSVATSSSAVYKPPLKPPKGTRQPNKPRVRPTSRQPSKDLGYSNYGPSIAYQTKSPVPLECPTACSCNLQISDLGLNVNCQERKIESIAELQPKPYNPKKMYLTENYIAVVRRTDFLEATGLDLLHLGNNRISMIQDRAFGDLTNLRRLYLNGNRIERLSPELFYGLQSLQYLFLQYNLIREIQSGTFDPVPNLQLLFLNNNLLQAMPSGVFSGLTLLRLNLRSNHFTSLPVSGVLDQLKSLIQIDLHDNPWDCTCDIVGMKLWVEQLKVGVLVDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTGAPASLGAGGGASS


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 56B.

TABLE 56BComparison of the NOV56 protein sequences.NOV56b VDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTNOV56c VDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTNOV56d VDEVICKAPKKFAETDMRSIKSELLCPDYSDVVVSTPTPSSIQVPARTSAVTPAVRLNSTNOV56a GAPASLGAGGGASSVPLSVLILSLLLVFIMSVFVAAGLFVLVMKRRKKNQSDHTSTNNSDNOV56b GAPASLGAGGGASSLE--------------------------------------------NOV56c GAPASLGAGGGASSVPLSVLILSLLLVFIMSVFVAAGLFVLVMKRRKKNQSDHTSTNNSDNOV56d GAPASLGAGGGASS----------------------------------------------NOV56a VSSFNMQYSVYGGGGGTGGHPHAHVHHRGPALPKVKTPAGHVYEYIPHPLGHMCKNPIYRNOV56b ------------------------------------------------------------NOV56c VSSFNMQYSVYGGGGGTGGHPHAHVHHRGPALPKVKTPAGHVYEYIPHPLGHMCKNPIYRNOV56d ------------------------------------------------------------NOV56a SREGNSVEDYKDLHELKVTYSSNHHLQQQQQPPPPPQQPQQQPPPQLQLQPGEEERRESHNOV56b ------------------------------------------------------------NOV56c SREGNSVEDYKDLHELKVTYSSNHHLQQQQQPPPPPQQPQQQPPPQLQLQPGEEERRESHNOV56d ------------------------------------------------------------NOV56a HLRSPAYSVSTIEPREDLLSPVQDADRFYRGILEPDKHCSTTPAGNSLPEYPKFPCSPAANOV56b ------------------------------------------------------------NOV56c HLRSPAYSVSTIEPREDLLSPVQDADRFYRGILEPDKHCSTTPAGNSLPEYPKFPCSPAANOV56d ------------------------------------------------------------NOV56a YTFSPNYDLRRPHQYLHPGAGDSRLREPVLYSPPSAVFV---------------------NOV56b ------------------------------------------------------------NOV56c YTFSPNYDLRRPHQYLHPGAGDSRLREPVLYSPPSAVFVEPNRNEYLELKAKLNVEPDYLNOV56d ------------------------------------------------------------NOV56a ------------NOV56b ------------NOV56c EVLEKQTTFSQFNOV56d ------------NOV56a (SEQ ID NO: 1124)NOV56b (SEQ ID NO: 1126)NOV56c (SEQ ID NO: 1128)NOV56d (SEQ ID NO: 1130)


Further analysis of the NOV56a protein yielded the following properties shown in Table 56C.

TABLE 56CProtein Sequence Properties NOV56aSignalP analysis:Cleavage site between residues 41 and 42PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 11; pos. chg 0; neg. chg 1H-region:length 1; peak value 0.00PSG score:−4.40GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −2.49possible cleavage site: between 38 and 39>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 665-681−13.59PERIPHERALLikelihood = 2.44 (at 571)ALOM score: −13.59 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 672Charge difference: 2.5 C(4.5)-N(2.0)C > N: C-terminal side will be inside>>> membrane topology: type 1b (cytoplasmic tail 665 to 925)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):2.75Hyd Moment(95):4.92G content:0D/E content:2S/T content:2Score: −7.05Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: KRRK (5) at 690pat4: RRKK (5) at 691pat7: nonebipartite: nonecontent of basic residues: 9.2%NLS Score: 0.15KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 670LL at 671checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):69.6%: nuclear 8.7%: mitochondrial 8.7%: cytoplasmic 4.3%: vacuolar 4.3%: plasma membrane 4.3%: vesicles of secretory system>> prediction for CG92715-01 is nuc (k = 23)


A search of the NOV56a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 56D.

TABLE 56DGeneseq Results for NOV56aNOV56aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABU12071Human NOV16b CG92715-02 protein1 . . . 925925/925(100%)0.0SEQ ID 62 - Homo sapiens, 972 aa.15 . . . 939 925/925(100%)[WO200281625-A2, 17-OCT-2002]ABU12070Human NOV16a CG92715-01 protein1 . . . 925925/925(100%)0.0SEQ ID 60 - Homo sapiens, 925 aa.1 . . . 925925/925(100%)[WO200281625-A2, 17-OCT-2002]AAU98780Human haematopoietic cell line Kg-1a1 . . . 925925/925(100%)0.0protein KIAA0918 - Homo sapiens, 9581 . . . 925925/925(100%)aa. [WO200236804-A2, 10-MAY-2002]AAB95753Human protein sequence SEQ ID1 . . . 925925/925(100%)0.0NO: 18665 - Homo sapiens, 958 aa.1 . . . 925925/925(100%)[EP1074617-A2, 07-FEB-2001]ABB12025Human IGFALS homologue, SEQ ID1 . . . 925924/925(99%)0.0NO: 2395 - Homo sapiens, 977 aa.20 . . . 944 924/925(99%)[WO200157188-A2, 09-AUG-2001]


In a BLAST search of public sequence databases, the NOV56a protein was found to have homology to the proteins shown in the BLASTP data in Table 56E.

TABLE 56EPublic BLASTP Results for NOV56aNOV56aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueO94991Hypothetical protein KIAA0918 -1 . . . 925925/925(100%)0.0Homo sapiens (Human), 966 aa9 . . . 933925/925(100%)(fragment).CAD48653Sequence 18 from Patent22 . . . 925 904/904(100%)0.0WO0220569 - Homo sapiens1 . . . 904904/904(100%)(Human), 937 aa.BAC67208Neuronal transmembrane protein1 . . . 925895/926(96%)0.0Slitrk5 - Mus musculus (Mouse),1 . . . 924908/926(97%)957 aa.Q9H156BG115M3.1 (Novel protein)46 . . . 925 423/886(47%)0.0(SLITL1 protein) - Homo sapiens27 . . . 812 563/886(62%)(Human), 845 aa.Q8NBC7Hypothetical protein FLJ33696 -46 . . . 781 393/742(52%)0.0Homo sapiens (Human), 724 aa27 . . . 717 511/742(67%)(fragment).


PFam analysis predicts that the NOV56a protein contains the domains shown in the Table 56F.

TABLE 56FDomain Analysis of NOV56aIdentities/PfamNOV56a MatchSimilarities forExpectDomainRegionthe Matched RegionValueLRR154 . . . 1778/25(32%)0.05321/25(84%)LRRCT235 . . . 28518/54(33%)2.7e−0834/54(63%)LRRNT373 . . . 40610/36(28%)0.009919/36(53%)LRR434 . . . 4578/25(32%)0.2817/25(68%)LRR458 . . . 48112/25(48%)0.002223/25(92%)LRR482 . . . 5055/25(20%)0.5919/25(76%)LRR506 . . . 52912/25(48%)0.007719/25(76%)LRRCT563 . . . 61310/54(19%)1.9e−0536/54(67%)


Example 57

The NOV57 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 57A.

TABLE 57ANOV57 Sequence AnalysisNOV57a, CG92813-01SEQ ID NO: 113115603 bpDNA SequenceORF Start: ATG at 518ORF Stop: TGA at 15401CTAATAGAATTCAGCGGCCGCTTTCCCCGGTGCGCAGTTGTGCTTGGACGTTTGTTCCTCCCTCTTCACGCTCTTCGCTGCGGGTAAGTTCTAAAGTTTCTGAAGGCCGTTCTTTGCAATGATTCCTCATATACCTTAGATACAGGCAACTTCTCCCAACTCTCATCCACCCGGGTGAAAACGCTCAGACTATCTGGATTCAAAAACAAAGTAAAAGGGGGCATATATAAGAGGCTTGAGAAACTTTTCTGGGAACTCAGCTCACAGGAGTGTCCCGCGGAATGCCCTGCCGCTTTTCGCCACAGCATCTCTCTTGCACTCCGCGTTCAACTGGCTACCTAGAGTCTTTTGCTGATGCTACTTGCTTTTGCCGGACTGGAGGTTCTTTGAAATAGCAGAGGTCTCAGACCAAGCCGTCAGCTGAATCTTTGCTGGCGCTCCTTAATCCCTGTAAATATCATTGCGTTTGCTTCACCCCTTCCTTCTCTTTATCACATCGTTTTAGGGAGCCAGGACCATGGACTTAGCACCAGACAGGGCTACTGGCCGCCCGTGGCTCCCGTTGCACACTCTATCAGTATCTCAGCTCCTTCGAGTGTTTTGGCTACTGTCATTGCTTCCGGGGCAGGCCTGGGTCCACGGGGCCGAGCCGCGCCAGGTGTTCCAAGTGCTGGAAGAGCAACCTCCAGGCACTCTGGTAGGCACCATCCAGACGCGCCCCGGCTTCACCTACAGGCTCAGCGAAAGCCACGCCCTGTTTGCCATAAACAGTAGCACCGGAGCCCTGTACACCACCTCCACCATCGACCGCGAGAGCCTGCCCAGCGACGTGATCAACCTGGTGGTCCTTTCCAGCGCGCCCACCTACCCCACCGAAGTGCGAGTGCTGGTGCGGGACCTCAATGACAACGCCCCCGTTTTCCCGGACCCCTCTATCGTGGTCACTTTCAAGGAAGACAGTAGCAGCGGACGCCAAGTCATCTTAGACACCGCCACCGACTCGGACATCGGCTCAAACGGTGTGGACCACCGCTCCTACCGCATCATCCGCGGCAATGAGGCGGGGCGCTTCCGTCTGGACATCAACCTGAACCCGAGCGGCGAGGGAGCGTTCCTGCATCTGGTGTCCAAGGGCGGACTGGACCGTGAGGTCACTCCGCAGTACCAGCTCCTGGTTGAGGTGGAGGACAAGGGTGAGCCTAAGCGGCGGGGCTACCTTCAGGTAAACGTGACTGTGCAAGACATTAATGACAACCCCCCGGTTTTTGGCAGTTCTCACTACCAGGCGGGGGTGCCTGAGGACGCGGTTGTGGGTTCCAGCGTCCTCCAGGTGGCGGCGGCGGACGCGGACGAGGGCACCAACGCGGACATCCGCTATCGCCTGCAGGACGAGGGGACCCCCTTCCAAATGGACCCTGAGACGGGACTTATCACGGTGCGGGAGCCCCTGGACTTCGAAGCTCGGCGCCAATACTCGCTTACGGTGCAGGCGATGGACAGAGGCGTGCCTTCCCTCACTGGGCGCGCCGAGGCGCTGATTCAGCTGCTGGACGTGAATGACAATGACCCGGTAGTGAAGTTCCGCTACTTCCCGGCCACCTCGOGCTACGCCTCGGTAGATGAGAATGCTCAAGTGGGCACCGTGGTGGCTCTGCTCACCGTGACGGACGCAGATTCTCCCGCGGCCAACGGGAACATCTCCGTGCAAATTCTCGGGGGCAATGAGCAGCGCCACTTTGAAGTGCAAAGCAGCAAAGTGCCGAACCTGAGCCTAATCAAGGTGGCCAGCGCCTTGGACCGCGAGCGCATCCCTTCCTACAACCTCACAGTTTCCGTCTCTGATAACTACGGGGCGCCCCCTGGCGCAGCAGTCCAGGCGCGCTCTTCTGTGGCAAGCCTGGTGATTTTTGTTAATGACATCAATGACCATCCTCCTGTCTTTTCACAGCAAGTGTACAGAGTGAACCTGAGCGAGGAGGCGCCTCCGGGAAGCTATGTGAGTGGGATATCTGCCACTGATGGCGACTCTGGTCTCAATGCTAATCTGCGTTACAGCATTGTCTCTGGCAATGGACTGGGATGGTTCCATATCAGTGAACATAGCGGCCTCGTGACCACTGGGTCCTCTGGGGGCCTGGACCGTGAACTTGCTTCCCAGATTGTTCTGAATATAAGTGCCCGGGACCAGGGAGTTCACCCCAAGGTGTCCTATGCCCAGCTTGTAGTAACTCTCCTAGATGTGAATGATGAAAAGCCAGTATTTAGCCAGCCAGAAGGGTATGATGTGTCTGTGGTTGAGAATGCCCCAACAGGGACAGAACTGTTGATGCTCAGGGCAACTGACGGGGACCTGGGTGACAACGGAACAGTGCGCTTCTCCTTACAAGAGGCAGAGACTGACCGGAGGTCCTTCCGTCTGGATCCTGTGTCTGGGAGGTTGAGTACTATTTCCTCCTTGGACAGAGAAGAGCAAGCCTTCTACTCCCTGTTGGTTCTGGCCACAGATCTGGGCTCCCCTCCCCAGTCATCAATGGCTCGCATAAATGTGAGTCTTCTGGATATAAATGATAACAGCCCTGTCTTCTACCCGGTCCAATACTTTGCTCACATTAAGGAGAATGAGCCTGGAGGTAGCTACATCACCACTGTGTCTGCCACTGACCCAGACTTGGGTACCAATGGTACTGTCAAATATAGCATATCTGCTGGGGACAGGTCTCGGTTTCAGGTCAATGCTCAGAGTGGGGTTATTTCTACAAGAATGGCCCTAGACAGAGAAGAAAAAACAGCTTATCAGTTGCAAATAGTAGCTACTGATGGTGGCAATTTACAATCTCCCAACCAGGCAATAGTAACCATCACTGTATTGGACACTCAAGACAACCCACCTGTATTCAGTCAGGTTGCCTACAGCTTTGTGGTTTTTGAGAACGTGGCGCTGGGATATCATGTGGGTAGTGTGTCTGCATCCACCATGGATCTCAATTCCAACATCAGTTATCTCATTACTACTGGGGATCAGAAAGGTATGTTTGCTATCAACCAGGTCACTGGGCAGCTTACCACAGCAAATGTGATTGATAGAGAAGAGCAATCCTTTTATCAGCTGAAGGTAGTGGCCAGTGGGGGCACAGTGACTGGAGACACTATGGTTAACATAACAGTTAAGGATTTGAATGACAACTCTCCCCATTTCCTTCAGGCAATAGAGAGTGTAAATGTGGTGGAGAATTGGCAGGCAGGTCACAGCATTTTCCAGGCCAAAGCTGTGGACCCTGATGAAGGTGTCAATGGCATGGTACTCTATAGTCTGAAGCAAAACCCCAAGAACCTGTTTGCTATCAATGAAAAGAATGGCACTATTAGTCTGCTTGGGCCCCTGGATGTTCATGCTGGCTCCTACCAAATAGAGATCTTGGCATCTGACATGGGTGTCCCACAGCTCTCCTCTAGTGTCATCCTAACAGTTTATGTCCATGATGTAAATGACAATTCACCAGTGTTTGACCAACTCTCTTATGAAGTCACCCTTTCTGAGTCAGAACCTGTGAATTCTCGATTCTTTAAAGTACAAGCTTCTGATAAGGATTCAGGAGCAAATGATGGTCAATTGTATATAAAAAGTGAACTGGACCGTGAACTTCAAGACAGATATGTTTTAATGGTTGTTGCTTCTGACAGAGCAGTGGAACCCCTTAGTGCTACTGTGAATGTTACTGTAATTTTAGAAGATGTAAATGATAACAGACCTCTTTTTAACAGTACCAATTACACATTTTACTTCGAAGAAGAGCAGAGGGCTGGGTCGTTTGTGGGCAAAGTAAGTGCTGTAGATAAAGACTTTGGGCCAAATGGAGAAGTAAGGTATTCTTTTGAAATGGTGCAGCCAGATTTTGAGTTGCATGCCATCAGTGGGGAAATTACAAATACTCATCAGTTTGACAGGGAGTCTCTTATGAGGCGGAGAGGGACTGCTGTGTTTAGCTTTACAGTCATAGCAACAGATCAGGGGATCCCTCAGCCTCTCAAGGATCAGGCCACTGTACATGTTTACATGAAGGATATAAATGATAATGCTCCCAAATTTTTAAAAGACTTTTACCAAGCTACAATATCAGAATCAGCAGCCAATCTGACACAAGTGTTAAGAGTATCTGCCTCAGATGTTGATGAAGGTAATAATGGACTTATTCACTATTCTATAATAAAAGGAAATGAAGAAAGACAGTTTGCTATAGACAGTACCTCTGGTCAGGTAACACTAATTGGCAAATTAGACTATGAAGCAACACCTGCCTATTCCCTTGTAATTCAAGCAGTGGATTCAGGGACAATCCCCCTCAATTCAACGTGTACTTTAAATATTGATATTTTAGATGAAAATGACAATACCCCTTCTTTCCTTAAATCAACACTGTTTGTTGATGTTTTGGAAAACATGAGAATTGGTGAACTCGTGTCCTCTGTTACTGCAACTGATTCCGATTCAGGTGACAATGTTGATTTATATTACAGTATTACTGGGACTAACAACCACGGAACTTTTAGCATTAGCCCAAACACTGGGAGTATTTTTCTTGCCAAAAAACTGGACTTTGAAACACAGTCTTTGTATAAATTAAATATAACAGCAAAAGACCAAGGAAGACCTCCTCGTTCATCTACAATGTCAGTGGTTATTCACGTGAGGGACTTTAATGACAATCCTCCTAGCTTTCCTCCTGGAGATATTTTCAAGTCTATTGTTGAGAACATTCCCATTGGTACATCTGTCATTTCAGTGACTGCACATGACCCTGATGCAGACATTAATGGTCAACTATCCTACACAATCATTCAACAGATGCCAAGAGGCAACCACTTTACCATAGATGAAGTCAAAGGGACTATATATACTAATGCTGAAATAGATCGGGAATTTGCTAATCTCTTTGAGTTGACTGTAAAAGCCAATGATCAAGCTGTGCCAATAGAAACTAGACGGTATGCTTTGAAGAACGTGACCATTTTGGTTACAGACCTCAATGACAATGTCCCAATGTTTATATCACAAAACGCCCTTGCTGCAGACCCATCAGCTGTGATTGGTTCCGTTCTGACAACAATTATGGCTGCTGACCCAGATGAAGGTGCTAATGGAGAAATAGAGTATGAGATCATCAATGGGGACACAGACACCTTCATTGTTGATCGTTATAGTGGAGACCTGAGAGTGGCTTCAGCGTTGGTGCCTTCACAGTTGATCTACAATCTCATAGTTTCAGCAACAGACCTTGGGCCTGAAAGGAGGAAATCGACCACTGAATTGACCATCATTCTTCAGGGCCTTGATGGACCTGTTTTTACTCAACCCAAATATATAACTATTTTGAAGGAAGGAGAACCCATTGGCACAAACGTGATATCAATAGAAGCAGCTAGCCCCAGAGGATCTGAGGCCCCAGTGGAGTATTATATTGTTTCAGTTCGTTGTGAAGAAAAAACTGTTGGACGCCTCTTTACTATTGGACGACATACTGGTATAATTCAGACCGCAGCCATTCTGGACCGGGAGCAAGGAGCATGTCTTTACCTGGTGGATGTTTATGCCATAGAAAAATCAACTGCTTTTCCCAGAACACAGAGAGCAGAGGTAGAAACAACACTTCAGGATATCAATGACAATCCACCAGTATTTCCAACGGACATGCTGGATCTCACGGTAGAGGAGAACATTGGAGATGGCTCTAAGATTATGCAGCTGACAGCCATGGATGCTGACGAGGTGCAAATGCTCTCGTCACATACACTATCATTAGTGGGTTCTTTGGTAGCAGCCATTTTAGCCACGGATGATGACTCTGGTGTGAATGGAGAAATTACATATATTGTGAATGAAGATGATGAAGATGGCATCTTTTTCCTGAATCCTATTACTGGGGTCTTTAATTTGACTCGATTATTAGATTATGAAGTACAGCAATATTATATCCTCACTGTTCGAGCAGAAGATGGTGGGGGACAATTTACTACCATCAGAGTTTATTTCAATATTCTAGATGTAAATGATAATCCACCTATTTTCAGCTTGAATTCATACAGCACATCTTTAATGGAGAATCTACCTGTGGGATCTACTGTTCTTGTGTTTAATGTTACTGATGCAGATATGATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAGTGAGAACAAAGACACAACATACCAGAATCTCTGGGACACATTCAAAGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGAGAAAGCAGGAAAGATCCAAAATTGACACCCTAACATCACAATTAAAAGAACTAGAAAAGCAAGAGCAAACACATTCAAAAGCTAGCAGAAGGCAAGAAATAACTAAAATCAGAGCAGAACTGAAGGATATAGAGACACAAAAAACCCTTCAAAAAATTAATGAATCCAGGAGCTGGTTTTTTGAAAGGATCAACAAAATTGATAGACCGCTAGCAAGACTAATAAAGAAGAAAACAGAGAAGAATCAAATAGACGCAATAAAAAATGATAAAGGGGATATCACCATCGATCCCACAGAAATACAAACTACCATCAGAGAATACTGCAAACACCTCTATGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACATACACCCTCCCAAGACTAAACCAGGAAGAAGTTGAATCTCTGAATAGACCAATAACAGACTCTGAAACTGTGGCAATAATCAATAGCTTACCAACCAAAAAGAGTCCAGGACCAGATGGATTCACAGCCGAATTCTACCAGATGATAACAACCCCAGTCTTTGCACAAGCTTTGTATAAAGTGGAGATTAATGAAAACACACTTACTGGAACAGATATAATACAAGTGTTCGCAGCAGATGGAGATGAAGGCACAAATGGACAGGTTCGCTATGGCATTGTTAATGGTAATACCAATCAGGAATTTCGGATAGACTCTGTCACAGGTGCCATCACTGTCGCTAAACCTTTGGATAGAGAAAAGACCCCTACCTACCATTTAACTGTTCAGGCAACAGATCGAGGCAGCACACCCAGAACTGATACCTCCACGGTCAGCATTGTTCTACTGGATATTAATGACTTTGTTCCTGTATTTGAGCTATCTCCATATTCTGTAAATGTCCCTGAGAATTTAGGGACACTACCCAGAACAATTCTTCAGACTGCTTCGCCTTGCGTGAGGTTTGCCAGCGCCAGTAAAGCGTATTTCACAACAATTCCTGAGGATGCACCAACTGGAACAGATGTTTTATTGGTAAATGCCTCAGATGCTGATGCTTCAAAGAATGCAGTTATAAGTTATAGGATCATCGGTGGAAACTCTCAGTTCACGATCAACCCATCGACAGGACAAATCATCACCAGCGCATTGTTAGATAGGGAAACAAAAGATAATTATACTTTGGTAGTGGTCTGCAGTGATGCGGGATCCCCAGAGCCTCTTTCCAGTTCCACCAGTGTGCTTGTCACTGTGACTGATGTCCATGACAATCCACCAAGATTTCAGCATCACCCATATGTCACTCACATCCCATCTCCTACTCTTCCAGGTTCCTTTGTCTTTGCGGTTACAGTCACAGATGCTGATATTGGACCAAATTCTGAACTGCATTATTCTCTTTCGGGTAGAAATTCTGAAAAATTTCACATTGACCCACTGAGGGGAGCCATTATGGCCGCCGGACCACTAAACGGAGCTTCAGAAGTGACATTTTCTGTGCATGTAAAAGATGGTGGCTCATTTCCAAAGACAGATTCTACAACAGTGACTGTTAGATTCGTGAATAAGGCCGATTTCCCTAAAGTCAGAGCCAAAGAACAAACGTTCATGTTTCCTGAAAACCAACCAGTCAGCTCTCTTGTCACCACCATCACAGGATCCTCTTTAAGAGGAGAACCTATGTCATATTATATCGCAAGTGGGAATCTTGGCAATACTTTCCAGATTGATCAGTTAACAGGGCAGGTGTCTATTAGTCAACCTCTGGATTTTGAAAAGATACAAAAATATGTTGTATGGATAGAGGCCAGAGACGGTGGTTTCCCTCCTTTCTCCTCTTACGAGAAACTTGATATAACAGTATTAGATGTCAATGATAATGCCCCAATTTTTAAGGAAGACCCATTTATATCTGAAATATTGGAAAACCTTTCCCCTCGAAAAATACTTACTGTTTCGGCAATGGACAAGGACAGTGGACCCAATGGACAGTTAGATTATGAAATTGTTAATGGCAACATGGAAAATAGTTTCAGTATCAATCATGCTACTGGTGAAATTAGAAGCGTTAGACCTTTGGACAGGGAAAAAGTATCTCATTATGTCCTAACCATAAAATCATCAGACAAAGGGTCCCCGTCTCAGAGTACTTCAGTAAAAGTCATGATTAACATTTTAGATGAAAATGATAATGCCCCTAGGTTTTCTCAGATATTTAGTGCCCATGTTCCTGAAAATTCCCCCTTAGGATACACAGTTACCCGTGTCACAACTTCTGATGAAGACATTGGGATCAATGCAATTAGTAGATATTCTATAATGGATGCAAGTCTTCCATTTACAATTAATCCCAGCACAGGGGATATTGTCATAAGCAGACCTTTAAATAGGGAAGATACAGACCGTTACAGAATTCGAGTTTCCGCACATGATTCTGGGTGGACTGTAAGTACAGATGTCACAATATTTGTGACAGACATCAATGACAATGCTCCAAGATTTAGCAGAACTTCCTATTATTTAGATTGCCCTGAACTTACTGAGATTGGCTCCAAAGTAACTCAGGTATTTGCAACAGATCCTGATGAGGGATCAAATGGACAAGTGTTTTATTTCATAAAATCCCAATCAGAATATTTCAGGATTAATGCCACCACTGGAGAGATTTTCAATAAACAGATCTTAAAATACCAAAATGTCACTGGCTTCAGTAATGTGAATATCAACAGGCATAGTTTTATAGTGACATCTTCAGATCGAGGTAAACCTTCCTTAATTAGTGAGACAACAGTTACCATCAATATAGTGGACAGTAATGACAATGCACCTCAATTTCTTAAAAGTAAATATTTCACTCCAGTCACCAAAAATGTTAAGGTTGGTACGAAGTTAATCAGAGTTACAGCAATAGATGACAAAGATTTTGGACTGAATTCAGAAGTGGAGTATTTCATTTCTAATGATAACCATTTAGGAAAATTTAAGTTGGACAATGATACGGGGTGGATTTCAGTAGCATCCTCCCTGATTTCTGACTTGAACCAAAACTTTTTTATCACAGTCACTGCAAAGGATAAGGGAAACCCTCCACTTTCTTCCCAAGCAACTGTTCACATAACTGTCACTGAGGAAAACTACCATACACCTGAATTCTCTCAAAGCCACATGAGTGCAACCATCCCTGAGAGCCATAGCATTGGGTCCATTGTCAGAACTGTTTCTGCAAGAGATAGAGATGCAGCGATGAATGGCTTGATTAAGTACAGCATTTCTTCAGGAAATGAAGAAGGCATTTTTGCAATCAATTCTTCTACAGGTATATTAACACTAGCCAAAGCTCTTGATTATGAGCTATGCCAGAAACACGAAATGACGATTAGTGCTATAGATGGAGGATGGGTTGCAAGAACTGGTTACTGCAGTGTGACCGTAAATGTGATTGATGTGAATGATAATTCTCCAGTATTCCTCTCTGATGACTATTTCCCTACTGTTTTGGAAAATGCCCCAAGTGGAACAACAGTTATCCACCTAAATGCAACAGATGCTGACTCTGGAACAAATGCTGTGATTGCGTATACTGTACAGTCATCTGACAGTGACCTCTTTGTCATTGACCCTAACACAGGAGTCATAACCACTCAAGGCTTCTTGGATTTTGAAACCAAGCAGAGCTACCATCTTACTGTGAAAGCCTTCAATGTCCCCGATGAGGAAAGGTGTAGCTTTGCCACTGTTAATATACAATTAAAAGGGACAAATGAATATGTGCCCCGTTTTGTTTCCAAACTTTACTATTTTGAAATCTCAGAAGCAGCTCCTAAAGGTACTATTGTTGGAGAAGTGTTTGCTAGCGACCGTGATTTGGGCACTGATGGGGAGGTACACTATTTGATTTTTGGTAATAGTCGAAAGAAGGGTTTCCAGATCAATAAGAAGACTGGACAGATTTATGTTTCTGGAATTCTTGATCGAAAAAAAGAAGAAAGGGTGTCTTTGAAGGTATTGGCCAAGAACTTTGGCAGCATTAGAGGTGCAGATATAGATGAGGTCACTGTAAATGTCACCGTGCTTGATGCAAATGACCCACCCATTTTTACTCTAAACATCTACAGTGTGCAGATCAGTGAAGGGGTCCCAATAGGAACTCATGTGACCTTTGTCAGTGCCTTTGACTCAGACTCCATCCCCAGCTGGAGCAGGTTTTCTTACTTCATCGGATCAGGGAATGAAAATGGTGCCTTTTCTATTAATCCGCAGACAGGACAGATCACCGTTACTGCAGAATTAGATCGAGAAACCCTTCCCATCTATAATCTCTCAGTTTTGGCTGTTGATTCAGGGACCCCCTCAGCTACAGGTAGTGCCTCTTTATTAGTCACCCTGGAAGATATAAATGATAACGGGCCCATGCTGACTGTCAGTGAAGGAGAAGTCATGGAAAACAAACGGCCAGGCACTTTGGTGATGACCCTTCAGTCCACTGACCCTGATCTCCCTCCAAATCAAGGTCCCTTTACTTATTACTTGCTGAGCACAGGTCCTGCCACCAGTTATTTCAGTCTGAGCACTGCTGGAGTTCTGAGCACAACCAGAGAGATTGACAGAGAGCAGATTGCAGACTTCTATCTGTCTGTGGTTACCAAGGATTCTGGTGTTCCTCAAATGTCTTCCACAGGAACTGTGCATATCACAGTTATAGACCAAAATGACAATCCTTCACAGTCTCGGACGGTGGAGATATTTGTTAATTATTATGGTAACTTGTTTCCCGGTGGGATTTTAGGCTCTGTGAAGCCACAGGATCCAGATGTGTTAGACAGCTTCCACTGCTCCCTTACTTCAGGAGTTACCAGCCTCTTCAGTATTCCAGGGGGTACTTGTGATCTGAATTCCCAGCCAAGGTCCACAGATGGCACGTTTGATCTGACTGTCCTTAGCAATGATGGAGTTCACAGCACAGTCACGAGCAACATCCGAGTTTTCTTTGCTGGATTTTCCAATGCCACAGTGGATAACAGCATCTTACTTCGTCTCGGCGTACCAACAGTAAAGGACTTCTTGACCAACCACTATCTTCATTTTTTACGCATTGCCAGCTCACAGCTGACAGGCTTAGGGACTGCTGTGCAACTGTACAGTGCATATGAAGAGAACAATAGAACGTTTCTTTTGGCAGCTGTGAAGCGAAATCATAATCAGTATGTGAATCCCAGTGGCGTAGCCACCTTCTTTGAAAGCATCAAAGAGATCCTTCTCCGGCAGAGTGGAGTAAAGGTGGAATCTGTGGATCATGACTCCTGTGTGCATGGCCCATGTCAGAATGGAGGGAGCTGTCTACGAAGATTGGCTGTGAGCTCCGTATTAAAAAGCCGTGAGAGTCTTCCAGTCATCATCGTGGCAAATGAACCTCTGCAGCCTTTCTTATGCAAGTGTCTGCCAGGATATGCGGGTAGCTGGTGTGAAATAGATATAGATGAATGTCTTCCATCACCTTGCCACAGTGGTGGAACCTGTCACAATTTAGTGGGAGGATTTTCATGCAGCTGCCCAGATGGCTTCACTGGTAGGGCGTGTGAGAGAGATATCAATGAGTGCCTGCAGAGTCCTTGCAAGAATGGTGCCATCTGCCAGAATTTTCCAGGAAGCTTCAACTGTGTTTGCAAAACTGGATACACAGGTATGACAACGTTTGTACTTTTCTCACTAAGACTTGGAAAATGTGTGAATCTTCAGTCAATTACTGTGAATGCAACCCCTGCTTTAATGGTGGTTCCTGCCAAAGTGGTGTGGATTCTTATTATTGTCATTGTCCATTTGGTGTCTTTGGAACACTGCGAGTTGAACAGTTATGGATTTGAGGAGTTATCATACATGGAATTTCCAAGCTTGGACCCCAATAACAACTATATTTATGTCAAATTTGCCACGATTAAAAGTCATGCCTTATTGCTTTACAACTATGACAACCAGACAGGCGACCGGGCTGAGTTTTTGGCCCTTGAAATTGCCGAAGAAAGACTAAGATTCTCTTATAATTTAGGCAGTGGTACATATAAGCTCACCACCATGAAGAAGGTGTCAGATGGACATTTTCACACTGTGATTGCCAGGAGAGCAGGAATGGCAGCCTCCTTAACTGTGGACTCCTGTTCTGAGAACCAAGAGCCAGGATATTGTACTGTCAGTAATGTGGCAGTTTCAGATGACTGGACTCTTGATGTTCAGCCAAATAGAGTTACAGTTGGAGGTATCAGATCTCTAGAACCAATCCTTCAGAGAAGAGGACACGTGGAAAGCCATGATTTTGTTGGGTGTATAATGGAGTTTGCAGTCAATGGAAGGCCTCTGGAACCCAGCCAAGCTTTGGCAGCACAAGGCATCCTAGATCAGTATGGCGATTTTATTTCTTACTGTTTTAAAGAAAAAAAATGCAAAAAAGTATGCTTCACTGTTACTCCTGACACTGCCTTATCATTAGAAGGCAAAGGGCGCTTGGACTACCACATGAGTCAGAATGAGAAGCGGGAATATTTGTTAAGGCAAAGCTTACGAGGTGCCATGTTGGAGCCTTTTGGTGTGAACAGTCTGGAAGTAAAATTTAGGACCAGAAGCGAGAATGGCGTTTTAATCCATATCCAAGAAAGCAGCAATTACACTACTGTGAAGGGAATGTGTGAATCTTCAGTCAATTACTGTGAATGCAACCCCTGCTTTAATGGTGGTTCCTGCCAAAGTGGTGTGGATTCTTATTATTGTCATTGTCCATTTGGTGTCTTTGGAAAACACTGCGAGTTGAACAGTTATGGATTTGAGGAGTTATCATACATGGAATTTCCAAGCTTGGACCCCAATAACAACTATATTTATGTCAAATTTGCCACGATTAAAAGTCATGCCTTATTGCTTTACAACTATGACAACCAGACAGGCGACCGGGCTGAGTTTTTGGCCCTTGAAATTGCCGAAGAAAGACTAAGATTCTCTTATAATTTAGGCAGTGGTACATATAAGCTCACCACCATGAAGAAGGTGTCAGATGGACATTTTCACACTGTGATTGCCAGGAGAGCAGGAATGACTCTTGATGTTCAGCCAAATAGAGTTACAGTTGGAGGTATCAGATCTCTAGAACCAATCCTTCAGAGAAGAGGACACGTGGAAAGCCATGATTTTGTTGGGTGTATAATGGAGTTTGCAGTCAATGGAAGGCCTCTGGAACCCAGCCAAGCTTTGGCAGCACAAGGCATCCTAGATCAGTATGGCGATTTTATTTCTTACTGTTTTAAAGAAAAAAAATGCAAAAAGTATGCTTCACTTGGCCTCCATCTCGGGAAGCATAGCTTGGCCTCCATCTCAAAAACAGATCCCTCAGTGAAGATTGGCTGCCGTGGCCCGAACATTTGTGCCAGCAACCCCTGCTGGGGTGATTTGCTGTGCATTAATCAGTGGTATGCCTACAGGTGTGTCCCTCCTGGGGACTGTGCCTCCCACCCGTGCCAGAATGGTGGCAGCTGTGAGCCAGGCCTGCACTCCGGCTTCACCTGTAGCTGCCCAGACTCGCACACGGGAAGGACCTGTGAGATGGTGGTGGCCTGTCTTGGCGTCCTCTGTCCTCAGGGGAAGGTGTGCAAAGCTGGAAGTCCTGCGGGGCATGTCTGTGTTCTGAGTCAGGGCCCTGAAGAGATCTCTCTGCCTTTGTGGGCTGTGCCTGCCATCGTGGGCAGCTGCGCAACCGTCTTGGCCCTCCTGGTCCTTAGCCTGATCCTGTGTAACCAGTGCAGGGGGAAGAAGGCCAAAAATCCCAAAGAGGAGAAGAAACCGAAGGAGAAGAAGAAAAAGGGAAGTGAGAACGTTGCTTTTGATGACCCTGACAATATCCCTCCCTATGGGGATGACATGACTGTGAGGAAGCAGCCTGAAGGGAACCCAAAACCAGATATCATTGAAAGGGAAAACCCCTACCTTATCTATGATGAAACTGATATTCCTCACAACTCAGAAACCATCCCCAGCGCCCCTTTGGCATCTCCAGAGCAGGAGATAGAGCACTATGACATTGACAACGCCAGCAGCATCGCCCCTTCGGATGCAGACATCATTCAACACTACAAGCAGTTCCGCAGCCACACACCAAAATTTTCAATCCAGAGGCACAGTCCCCTAGGCTTTGCAAGGCAATCCCCCATGCCCTTAGGAGCAAGCAGTTTGACTTACCAGCCTTCATATGGTCAAGGTTTGAGAACCAGCTCCCTAAGCCACTCAGCATGCCCAACTCCCAACCCTCTGTCTCGACACAGTCCAGCCCCTTTCTCCAAATCTTCTACGTTCTATAGAAACAGCCCAGCAAGGGAATTGCATCTTCCTATAAGGGATGGTAATACTTTGGAAATGCATGGTGACACCTGCCAACCTGGCATTTTCAACTATGCCACAAGGCTGGGAAGGAGAAGCAAGAGTCCTCAGGCCATGGCATCACATGGTTCTAGACCAGGGAGTCGCCTAAAGCAGCCGATTGGGCAGATTCCACTGGAATCTTCTCCTCCAGTCGGACTTTCTATTGAAGAAGTGGAGAGGCTCAACACACCTCGCCCTAGAAACCCAAGTATCTGCAGTGCAGACCATGGGAGGTCTTCTTCAGAGGAGGACTGCAGAAGGCCACTGTCTAGAACAAGGAATCCAGCGGATGGCATTCCAGCTCCAGAATCCTCTTCTGATAGTGACTCCCATGAATCTTTCACTTGCTCAGAAATGGAATATGACAGGGAGAAGCCAATGGTATATACTTCCAGAATGCCCAAATTATCTCAAGTCAATGAATCTGATGCAGATGATGAAGATAATTATGGAGCCAGACTGAAGCCTCGAAGGTACCACGGTCGCAGGGCCGAGGGAGGACCTGTGGGCACCCAGGCAGCAGCACCAGGCACTGCTGACAACACACTGCCCATGAAGCTAGGGCAGCAAGCAGGGACTTTCAACTGGGACAACCTTTTGAACTGGGGCCCTGGCTTTGGCCATTATGTAGATGTTTTTAAAGATTTGGCATCTCTTCCAGAAAAAGCAGCAGCAAATGAAGAAGGCAAAGCTGGGACAACTAAACCAGTCCCCAAAGATGGGGAAGCAGAACAGTATGTGTGAAGTTTATGTACTGGCACTATAAAATATAAAAACAAGAAATAATACTCAAACCATTGTAAAGTTGCTGACTAGGTTGGGTCACATTTGAAAAACAGGCCAGTATGGACTAGTGGTGGAGGGAAAACTTTAAAAATAATAACCACAATGCTGCTGAAACAGACTCACAACAACTCTTAATTTAAACATGTGTGGTTGAATTCNOV57a, CG92813-01SEQ ID NO: 11324961 aaMW at 543673.9kDProtein SequenceMDLAPDRATGRPWLPLHTLSVSQLLRVFWLLSLLPGQAWVHGAEPRQVFQVLEEQPPGTLVGTIQTRPGFTYRLSESHALFAINSSTGALYTTSTIDRESLPSDVINLVVLSSAPTYPTEVRVLVRDLNDNAPVFPDPSIVVTFKEDSSSGRQVILDTATDSDIGSNGVDHRSYRIIRGNEAGRFRLDINLNPSGEGAFLHLVSKGGLDREVTPQYQLLVEVEDKGEPKRRGYLQVNVTVQDINDNPPVFGSSHYQAGVPEDAVVGSSVLQVAAADADEGTNADIRYRLQDEGTPFQMDPETGLITVREPLDFEARRQYSLTVQAMDRGVPSLTGRAEALIQLLDVNDNDPVVKFRYFPATSRYASVDENAQVGTVVALLTVTDADSPAANGNISVQILGGNEQRHFEVQSSKVPNLSLIKVASALDRERIPSYNLTVSVSDNYGAPPGAAVQARSSVASLVIFVNDINDHPPVFSQQVYRVNLSEEAPPGSYVSGISATDGDSGLNANLRYSIVSGNGLGWFHISEHSGLVTTGSSGGLDRELASQIVLNISARDQGVHPKVSYAQLVVTLLDVNDEKPVFSQPEGYDVSVVENAPTGTELLMLRATDGDLGDNGTVRFSLQEAETDRRSFRLDPVSGRLSTISSLDREEQAFYSLLVLATDLGSPPQSSMARINVSLLDINDNSPVFYPVQYFAHIKENEPGGSYITTVSATDPDLGTNGTVKYSISAGDRSRFQVNAQSGVISTRMALDREEKTAYQLQIVATDGGNLQSPNQAIVTITVLDTQDNPPVFSQVAYSFVVFENVALGYHVGSVSASTMDLNSNISYLITTGDQKGMFAINQVTGQLTTANVIDREEQSFYQLKVVASGGTVTGDTMVNITVKDLNDNSPHFLQAIESVNVVENWQAGHSIFQAKAVDPDEGVNGMVLYSLKQNPKNLFAINEKNGTISLLGPLDVHAGSYQIEILASDMGVPQLSSSVILTVYVHDVNDNSPVFDQLSYEVTLSESEPVNSRFFKVQASDKDSGANDGQLYIKSELDRELQDRYVLMVVASDRAVEPLSATVNVTVILEDVNDNRPLFNSTNYTFYFEEEQRAGSFVGKVSAVDKDFGPNGEVRYSFEMVQPDFELHAISGEITNTHQFDRESLMRRRGTAVFSFTVIATDQGIPQPLKDQATVHVYMKDINDNAPKFLKDFYQATISESAANLTQVLRVSASDVDEGNNGLIHYSIIKGNEERQFAIDSTSGQVTLIGKLDYEATPAYSLVIQAVDSGTIPLNSTCTLNIDILDENDNTPSFLKSTLFVDVLENMRIGELVSSVTATDSDSGDNVDLYYSITGTNNHGTFSISPNTGSIFLAKKLDFETQSLYKLNITAKDQGRPPRSSTMSVVIHVRDFNDNPPSFPPGDIFKSIVENIPIGTSVISVTAHDPDADINGQLSYTIIQQMPRGNHFTIDEVKGTIYTNAEIDREFANLFELTVKANDQAVPIETRRYALKNVTILVTDLNDNVPMFISQNALAADPSAVIGSVLTTIMAADPDEGANGEIEYEIINGDTDTFIVDRYSGDLRVASALVPSQLIYNLIVSATDLGPERRKSTTELTIILQGLDGPVFTQPKYITILKEGEPIGTNVISIEAASPRGSEAPVEYYIVSVRCEEKTVGRLFTIGRHTGIIQTAAILDREQGACLYLVDVYAIEKSTAFPRTQRAEVETTLQDINDNPPVFPTDMLDLTVEENIGDGSKIMQLTAMDADEVQMLSSHTLSLVGSLVAAILATDDDSGVNGEITYIVNEDDEDGIFFLNPITGVFNLTRLLDYEVQQYYILTVRAEDGGGQFTTIRVYFNILDVNDNPPIFSLNSYSTSLMENLPVGSTVLVFNVTDADMMKAEIKMFFETSENKDTTYQNLWDTFKAVCRGKFIALNAHKRKQERSKIDTLTSQLKELEKQEQTHSKASRRQEITKIRAELKDIETQKTLQKINESRSWFFERINKIDRPLARLIKKKTEKNQIDAIKNDKGDITIDPTEIQTTIREYCKHLYANKLENLEEMDKFLDTYTLPRLNQEEVESLNRPITDSETVATINSLPTKKSPGPDGFTAEFYQMITTPVFAQALYKVEINENTLTGTDIIQVFAADGDEGTNGQVRYGIVNGNTNQEFRIDSVTGAITVAKPLDREKTPTYHLTVQATDRGSTPRTDTSTVSIVLLDINDFVPVFELSPYSVNVPENLGTLPRTILQTASPCVRFASASKAYFTTIPEDAPTGTDVLLVNASDADASKNAVISYRIIGGNSQFTINPSTGQIITSALLDRETKDNYTLVVVCSDAGSPEPLSSSTSVLVTVTDVHDNPPRFQHHPYVTHIPSPTLPGSFVFAVTVTDADIGPNSELHYSLSGRNSEKFHIDPLRGAIMAAGPLNGASEVTFSVHVKDGGSFPKTDSTTVTVRFVNKADFPKVRAKEQTFMFPENQPVSSLVTTTTGSSLRGEPMSYYIASGNLGNTFQIDQLTGQVSISQPLDFEKIQKYVVWIEARDGGFPPFSSYEKLDITVLDVNDNAPIFKEDPFISEILENLSPRKILTVSAMDKDSGPNGQLDYEIVNGNMENSFSINHATGEIRSVRPLDREKVSHYVLTIKSSDKGSPSQSTSVKVMINILDENDNAPRFSQIFSAHVPENSPLGYTVTRVTTSDEDIGINAISRYSIMDASLPFTINPSTGDIVISRPLNPEDTDRYRIRVSAHDSGWTVSTDVTIFVTDTNDNAPRFSRTSYYLDCPELTEIGSKVTQVFATDPDEGSNGQVFYFIKSQSEYFRINATTGEIFNKQILKYQNVTGFSNVNINRHSFIVTSSDRGKPSLISETTVTINIVDSNDNAPQFLKSKYFTPVTKNVKVGTKLIRVTAIDDKDFGLNSEVEYFISNDNHLGKFKLDNDTGWISVASSL


Further analysis of the NOV57a protein yielded the following properties shown in Table 57C.

TABLE 57CProtein Sequence Properties NOV57aSignalP analysis:Cleavage site between residues 43 and 44PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 11; pos. chg 2; neg. chg 2H-region:length 14; peak value 5.01PSG score:0.61GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −0.05possible cleavage site: between 42 and 43>>> Seems to have a cleavable signal peptide (1 to 42)ALOM:Klein et al's method for TM region allocationInit position for calculation: 43Tentative number of TMS(s) for the threshold 0.5: 4INTEGRALLikelihood =Transmembrane 1753-1769 −0.59INTEGRALLikelihood =Transmembrane 2193-2209 0.26INTEGRALLikelihood =Transmembrane 3837-3853−11.25INTEGRALLikelihood =Transmembrane 4488-4504 −9.08PERIPHERALLikelihood =  1.38 (at 1566)ALOM score: −11.25 (number of TMSs: 4)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 21Charge difference: −0.5 C(1.0)-N(1.5)N >= C: N-terminal side will be inside>>> membrane topology: type 3aMITDISC:discrimination of mitochondrial targeting seqR content: 0Hyd Moment(75):8.27Hyd Moment(95):10.66G content:0D/E content: 2S/T content:0Score: −6.21Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: PKRR (4) at 228pat4: HKRK (3) at 1918pat4: KKPK (4) at 4521pat4: KKKK (5) at 4526pat4: KPRR (4) at 4869pat7: PKRRGYL (5) at 228pat7: PERRKST (5) at 1588pat7: PKEKKKK (5) at 4523bipartite: KKTGQIYVSGILDRKKE at 3227bipartite: KKAKNPKEEKKPKEKKK at 4512content of basic residues: 8.4%NLS Score: 3.22KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: foundKIDTLTSQL at 1926VAC: possible vacuolar targeting motif: foundTLPI at 3349RNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regions1912 I0.911913 A0.911914 L0.911915 N0.911916 A0.971917 H0.981918 K1.001919 R1.001920 K1.001921 Q1.001922 E1.001923 R1.001924 S1.001925 K1.001926 I1.001927 D1.001928 T1.001929 L1.001930 T1.001931 S1.001932 Q1.001933 L1.001934 K1.001935 E1.001936 L1.001937 E1.001938 K1.001939 Q1.001940 E1.001941 Q1.001942 T1.001943 H1.001944 S1.001945 K1.001946 A0.981947 S0.971948 R0.961949 R0.961950 Q0.961951 E0.961952 I0.921953 T0.731954 K0.59total: 43 residuesFinal Results (k = 9/23):47.8%: endoplasmic reticulum21.7%: nuclear13.0%: mitochondrial 4.3%: vacuolar 4.3%: Golgi 4.3%: plasma membrane 4.3%: vesicles of secretory system>> prediction for CG92813-01 is end (k = 23)


A search of the NOV57a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 57D.

TABLE 57DGeneseq Results for NOV57aNOV57aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueABU12072Human NOV17a CG92813-01 protein 1 . . . 49614892/4961(98%)0.0SEQ ID 64 - Homo sapiens, 4903 aa. 1 . . . 49034896/4961(98%)[WO200281625-A2, 17-OCT-2002]ABB59831Drosophila melanogaster polypeptide49 . . . 1878748/1932(38%)0.0SEQ ID NO 6285 - Drosophila71 . . . 19511066/1932(54%)melanogaster, 5147 aa.[WO200171042-A2, 27-SEP-2001]AAO26789Human cadherin (CAD) protein, SEQ364 . . . 3239 839/3033(27%)0.0ID No 12 - Homo sapiens, 3354 aa.39 . . . 29111324/3033(42%)[WO200299042-A2, 12-DEC-2002]AAO26793Human cadherin (CAD) protein, SEQ 5 . . . 1877621/1950(31%)0.0ID No 16 - Homo sapiens, 3298 aa.12 . . . 1883925/1950(46%)[WO200299042-A2, 12-DEC-2002]ABB05430Human dachsous protein SEQ ID 5 . . . 1877621/1950(31%)0.0NO: 2 - Homo sapiens, 3298 aa.12 . . . 1883925/1950(46%)[JP2001327295-A, 27-NOV-2001]


In a BLAST search of public sequence databases, the NOV57a protein was found to have homology to the proteins shown in the BLASTP data in Table 57E.

TABLE 57EPublic BLASTP Results for NOV57aNOV57aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueP33450Cadherin-related tumor suppressor49 . . . 1878748/1932(38%)0.0precursor (Fat protein) - Drosophila71 . . . 19511066/1932(54%)melanogaster (Fruit fly), 5147 aa.IJFFTMcadherin-related tumor suppressor49 . . . 1878744/1932(38%)0.0precursor - fruit fly (Drosophila71 . . . 19511065/1932(54%)melanogaster), 5147 aa.Q8CD68Hypothetical ATP/GTP-binding site4515 . . . 4961 422/448(94%)0.0motif A - Mus musculus (Mouse),3 . . . 448430/448(95%)448 aa.Q96JQ0Protocadherin 16 precursor 5 . . . 1877621/1950(31%)0.0(Cadherin 19) (Cadherin fibroblast12 . . . 1883925/1950(46%)1) - Homo sapiens (Human), 3298aa.Q8BM82Hypothetical cadherin domain1384 . . . 1900 393/518(75%)0.0containing protein - Mus musculus1 . . . 490420/518(80%)(Mouse), 575 aa.


PFam analysis predicts that the NOV57a protein contains the domains shown in the Table 57F.

TABLE 57FDomain Analysis of NOV57aIdentities/PfamNOV57aSimilarities forExpectDomainMatch Regionthe Matched RegionValuecadherin 47 . . . 12619/107(18%)0.01859/107(55%)cadherin140 . . . 24135/113(31%)6.6e−1577/113(68%)cadherin255 . . . 34442/107(39%)4.5e−2875/107(70%)cadherin363 . . . 46644/113(39%)3.2e−2379/113(70%)cadherin480 . . . 57337/109(34%)  9e−2575/109(69%)cadherin588 . . . 68046/108(43%)1.2e−2676/108(70%)cadherin694 . . . 78443/107(40%)1.5e−2668/107(64%)cadherin798 . . . 88433/107(31%)5.7e−1866/107(62%)cadherin898 . . . 98732/107(30%)7.5e−1865/107(61%)cadherin1001 . . . 107124/107(22%)1.2e−0858/107(54%)cadherin1085 . . . 118134/111(31%)7.6e−1471/111(64%)cadherin1195 . . . 128639/107(36%)  5e−2776/107(71%)cadherin1300 . . . 139142/107(39%)  5e−2771/107(66%)cadherin1405 . . . 150038/108(35%)6.1e−1973/108(68%)cadherin1506 . . . 160230/114(26%)2.3e−1271/114(62%)cadherin1614 . . . 171128/112(25%) 0.006963/112(56%)cadherin1754 . . . 184031/107(29%)2.4e−1364/107(60%)cadherin2107 . . . 219844/107(41%)4.7e−3176/107(71%)cadherin2244 . . . 233445/107(42%)2.6e−2873/107(68%)cadherin2348 . . . 243633/107(31%)2.8e−1266/107(62%)cadherin2449 . . . 253737/107(35%)  4e−1167/107(63%)cadherin2551 . . . 264136/107(34%)1.2e−2572/107(67%)cadherin2654 . . . 274038/107(36%)2.7e−1764/107(60%)cadherin2754 . . . 285132/116(28%)  4e−1675/116(65%)cadherin2865 . . . 295741/107(38%)1.26-1568/107(64%)cadherin2971 . . . 306236/107(34%)3.2e−2675/107(70%)cadherin3076 . . . 316436/108(33%)2.9e−2167/108(62%)cadherin3180 . . . 327333/107(31%)1.7e−1671/107(66%)cadherin3286 . . . 337837/107(35%)4.2e−2879/107(74%)cadherin3390 . . . 348238/109(35%)7.7e−2276/109(70%)EGF3743 . . . 377418/47(38%)2.2e−0825/47(53%)EGF3781 . . . 381215/47(32%)9.2e−0525/47(53%)laminin_G3890 . . . 403349/158(31%)6.4e−16101/158(64%)EGF4148 . . . 417916/47(34%)0.01424/47(51%)laminin_G4208 . . . 432246/156(29%)  9e−0585/156(54%)EGF4410 . . . 444216/47(34%)3.1e−0626/47(55%)


Example 58

The NOV58 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 58A.

TABLE 58ANOV58 Sequence AnalysisNOV58a, CG93387-05SEQ ID NO:11435432 bpDNA SequenceORF Start: ATG at 187ORF Stop: TAA at 3991GGTGCCGAGCACTCCGGACTCTACGTGAACAACAACGGGATCATCTCCTTCCTGAAGGAGGTTTCTCAGTTCACCCCAGTGGCCTTCCCCATTGCCAAGGACCGCTGCGTGGTGGCAGCCTTCTGGGCAGATGTGGACAACCGGCGTGCAGGCGACGTGTACTACCGGGAGGCCACCGACCCAGCCATGCTGCGCCGAGCCACGGAGGACGTCAGGCACTACTTCCCCGAGCTCCTGGACTTCAATGCCACCTGGGTTTTTGTTGCCACCTGGTACCGAGTGACCTTCTTTGGAGGCAGTTCCTCATCCCCTGTCAACACATTCCAGACTGTGCTCATCACAGACGGCAAGCTCTCCTTCACCATCTTCAACTATGAGTCCATCGTGTGGACCACAGGCACACACGCCAGCAGCGGGGGCAACGCCACTGGCCTCGGGGGCATCGCAGCCCAGGCTGGCTTCAACGCAGGCGATGGGCAGCGTTACTTCAGTATCCCCGGCTCGCGCACAGCAGACATGGCCGAGGTGGAGACCACCACCAACGTGGGTGTGCCCGGGCGCTGGGCGTTCAGAATCGATGATGCCCAGGTGCGCGTGGGGGGCTGCGGCCATACAACGTCCGTGTGCCTGGCCCTGCGCCCCTGCCTCAACGGCGGCAAGTGCATCGACGACTGCGTCACGGGCAACCCCTCCTACACCTGCTCCTGCCTCTCGGGCTTCACGGGGCGGAGGTGCCACCTGGACGTGAACGAATGTGCCTCCCAGCCCTGTCAGAATGGTGGGACCTGTACTCACGGCATCAACAGTTTCCGCTGCCAGTGCCCGGCTGGCTTTGGGGGACCCACCTGTGAGACAGCCCAATCCCCCTGTGACACCAAAGAGTGTCAACATGGTGGCCAGTGCCAGGTGGAGAACGGCTCTGCGGTGTGTGTGTGCCAGGCCGGATACACCGGAGCAGCCTGCGAGATGGATGTGGACGACTGCAGCCCTGACCCCTGCCTGAATGGAGGCTCTTGTGTTGACCTAGTGGGGAATTACACCTGCTTGTGTGCCGAGCCCTTCAAGGGACTTCGCTGTGAGACAGGAGACCATCCAGTGCCAGACGCCTGCCTCTCGGCCCCTTGCCACAATGGGGGCACCTGTGTGGATGCGGACCAGGGCTACGTGTGCGAGTGCCCCGAAGGCTTCATGGGCCTGGACTGCAGGGAGAGAGTCCCCGATGACTGTGAGTGCCGCAACGGAGGCAGATGCCTGGGCGCCAACACCACCCTCTGCCAGTGCCCCCTGGGATTCTTTGGGCTTCTCTGTGAATTTGAAATCACAGCCATGCCCTGCAACATGAACACACAGTGCCCAGATGGGGGCTACTGCATGGAGCACGGCGGGAGCTACCTCTGCGTCTGCCACACCGACCACAATGCCAGCCACTCCCTGCCATCACCCTGCGACTCGGACCCCTGCTTCAACGGAGGCTCCTGCGATGCCCATGACGACTCCTACACCTGCGAGTGCCCGCGCGGGTTCCACGGCAAGCACTGCGAGAAAGCCCGGCCACACCTGTGCAGCTCAGGGCCCTGCCGGAACGGGGGCACGTGCAAGGAGGCGGGCGGCGAGTACCACTGCAGCTGCCCCTACCGCTTCACTGGGAGGCACTGTGAGATCGGGAAGCCAGACTCGTGTGCCTCTGGCCCCTGTCACAACGGCGGCACCTGCTTCCACTACATTGGCAAATACAAGTGTGACTGTCCCCCAGGCTTCTCCGGGCGGCACTGCGAGATAGCCCCCTCCCCCTGCTTCCGGAGCCCGTGTGTGAATGGGGGCACCTGCGAGGACCGGGACACGGATTTCTTCTGCCACTGCCAAGCAGGGTACATGGGACGCCGGTGCCAGGCAGAGGTGGACTGCGGCCCCCCGGAGGAGGTGAAGCACGCCACACTGCGCTTCAACGGCACGCGGCTGGGCGCGGTGGCCCTGTATGCATGTGACCGTGGCTACAGCCTGAGCGCCCCCAGCCGCATCCGGGTCTGCCAGCCACACGGTGTCTGGAGTGAGCCTCCCCAGTGCCTTGAAATCGATGAGTGCCGGTCTCAGCCGTGCCTGCATGGGGGCTCTTGTCAGGACCGCGTTGCTGGGTACCTGTGCCTCTGCAGCACAGGCTATGAGGGCGCCCACTGTGAGCTGGAGAGGGATGAGTGCCGAGCTCACCCGTGCAGAAATGGAGGGTCCTGCAGGAACCTCCCAGGGGCCTATGTCTGCCGGTGCCCTGCAGGCTTCGTTGGAGTCCACTGTGAGACAGAGGTGGACGCCTGCGACTCCAGCCCCTGCCAGCATGGAGGCCGGTGTGAGAGCGGCGGCGGGGCCTACCTGTGCGTCTGCCCAGAGAGCTTCTTCGGCTACCACTGCGAGACAGTGAGTGACCCCTGCTTCTCCAGCCCCTGTGGGGGCCGTGGCTATTGCCTGGCCAGCAACGGCTCCCACAGCTGCACCTGCAAAGTGGGCTACACGGGCGAGGACTGCGCCAAAGAGCTCTTCCCACCGACGGCCCTCAAGATGGAGAGAGTGGAGGAGAGTGGGGTCTCTATCTCCTGGAACCCGCCCAATGGTCCAGCCGCCAGGCAGATGCTTGATGGCTACGCGGTCACCTACGTCTCCTCCGACGGCTCCTACCGCCGCACAGACTTTGTGGACAGGACCCGCTCCTCGCACCAGCTCCAGGCCCTGGCGGCCGGCAGGGCCTACAACATCTCCGTCTTCTCAGTGAAGCGAAACAGTAACAACAAGAATGACATCAGCAGGCCTGCCGTGCTGCTGGCCCGCACGCGACCCCGCCCTGTGGAAGGCTTCGAGGTCACCAATGTGACGGCTAGCACCATCTCAGTGCAGTGGGCCCTGCACAGGATCCGCCATGCCACCGTCAGTGGGGTCCGTGTGTCCATCCGCCACCCTGAGGCCCTCAGGGACCAGGCCACCGATGTGGACAGGAGTGTGGACAGGTTCACCTTTAGGGCCCTGCTGCCTGGGAAGAGGTACACCATCCAGCTGACCACCCTCAGTGGGCTCAGGGGAGAGGAGCACCCCACAGAGAGCCTGGCCACCGCGCCGACGCACGTGTGGACCCGGCCCCTGCCCAGGCAGCTTGCTGGAGGCTTATGTCATCAATGTGACCACCAGCCAGAGCACCAAGAGCCGCTATGTCCCCAACGGGAAGCTGGCGTCCTACACGGTGCGCGACCTGCTGCCGGGACGGCGGTACCAGCCCTCTGTGATAGCAGTGCAGAGCACGGAGCTCGGGCCGCAGCAGCACCAGGGAGGACACCACCCTCGGGTGCTCAAGAACAGACCGCCCCCGGCGCGCCTGCCGGAGCTGCGCCTGCTCAATGACCACAGCGCCCCCGAGACCCCCACCCAGCCCCCCAGGTTCTCGGAGTTTGTGGACGGCAGAGGAAGAGTGAGCGCCAGGTTCGGTGGCTCACCCAGCAAAGCAGCCACCGTGAGATCACAACCCACAGCCTCGGCGCAGCTCGAGAACATGGAGGAAGCCCCCAAGCGGGTCAGCCCGGCCCTCCAGCTCCCTGAACACGGCAGCAAGGACATCGGAAACGTCCCTGGCAACTGTTCAGAAAACCCCTGTCAGAACGGAGGCACTTGTGTGCCGGGCGCAGACGCCCACAGCTGTGACTGCGGGCCAGGGTTCAAAGGCAGACGCTGCGAGCTCGCCTGTATAAAGGTGTCCCGCCCCTGCACAAGGCTGTTCTCCGAGACAAAGGCCTTTCCAGTCTGGGAGGGAGGCGTCTGTCACCACGTGTATAAAAGAGTCTACCGAGTTCACCAAGACATCTGCTTCAAAGAGAGCTGTGAAAGCACAAGCCTCAAGAAGACCCCAAACAGGAAACAAAGTAAGAGTCAGACACTGGAGAAATCTTAAGGATTTAAGACGTTCTTGTTACACTCCACCAACCTCACGAGTTTCTAACACCCAGGAAGATGAGGTCTAAAAACTGGATGAAAAAGGACACCCTGAGAAAAGGTCCTAGCTGGAGTCAGTCCCCTCTGTGACCTCTCTCCTCAGGCCTCTAGAGGACAGATGGCCAGGCCTGTGCACACACCAGCCCACCCTGAGAGACCCCTCTGGGACCAACCACCTGTGAGTCCTGCGATGCGTTTAAGCAGCCTGTGCCCTCACCCAAGCTGCAGTTCCTGAAGGTGTAGTCTGTGTCTCTGCGGATGAGATGACAGCTCGCCATTCCCCGGAATCAGTGAGGCTGTCAGTCAGCCACGCTTCTGCAGTATGCAGAAACCTGTTCTTAGACTCCAAAGCCAGAGAAAGAATTCTCCCTTCGAGGCCCAACAAATTGAGAAGGAACTGTGATGGACCACTTCCAAAACAGAGACGGGGGCAGGGGCTGAAGGGCAGAGACCAGGTGATGTCAGAAGGAAAGCCGGGTTGCAGACACAGCCGCCCCTGCTCTGGTCCTCCAGCGTGTTTATGACGCTCGTGCAGGTCGACGAGCCATCCTATGGACTAGTTAACACTAAGGTGGAGTTCAGACTTTTTTAGACAACGGCGCGACTGGCAGCCTTTCTCTATCAAGGGTCAGACGGTAAACGTTTTCAGCTTTGCAGACCAGAGGTCCCTGTGGCTACAGTAGCGCAGACACAGCCACAGGCATGTCATTGAATGGCTGCGGCTATGTTCCAATAAAAACTTATTTACAATAACAGGTGGTGGCCAAATTGGCCCATGGGCCTTATTTGGTGAACCCTGTTCTATGAGATCACCTAGGCTTCAGCCTTAAACAGTGGAAGCCATCCCCTGAATGACAAGTCACAAGGGTATCAAAGAAAGACCCCTGAATTTTCATGGAAAAAGCTATTCAGACCCCTGCTTGGAAAGCTAAGGCACACTGCCACGAAGCAGCAAGGACGCCTTACAAGTCTCAGTGCAACAGAGATGGACACCTGGGCTGGGCTGGACAATGTTTAAGGTTCCTTTTAGTCCATGACTCAAGTGATACTGTTTTAGGCTATCAGCTAGTAAACACGATCTTAGACATCCCCATCTTTGTAAGCAGAACAGTACGGCACTTCACCACATCTGCTTCCCACCATGCTTCTAAGCAGCTGTCTTCCCCCTGCTAATGTTACAACCAAAGCAGCCACCCCACCTCCTCTCGTGTTGAGCCTCACGACCGCTGACCCAGCTGGAAAGCCAGCGCCCTGCCGCGTCACCCTGACTCTGCTCAGAGCCAGCATTCCAGCCACAAAGAGGGCCTCCTTCCTTTCCTCTTTCATAAAAATGTTTTTTGAAGAGTTAGAGTATATTTTAGGCTTTTTATCTTTATTAAAATTTCATGTGCATGTGTANOV58a, CG93387-05SEQ ID NO:11441268 aaMW at 136576.5 kDProtein SequenceMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTIFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGDHPVPDACLSAPCHNGGTCVDADQGYVCECPEGFMGLDCRERVPDDCECRNGGRCLGANTTLCQCPLGFFGLLCEFEITAMPCNMNTQCPDGGYCMEHGGSYLCVCHTDHNASHSLPSPCDSDPCFNGGSCDAHDDSYTCECPRGFHGKHCEKARPHLCSSGPCRNGGTCKEAGGEYHCSCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCVNGGTCEDRDTDFFCHCQAGYMGRRCQAEVDCGPPEEVKHATLRFNGTRLGAVALYACDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYEGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKELFPPTALKNERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQQHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPSKAATVRSQPTASAQLENNEEAPKRVSPALQLPEHGSKDIGNVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLEKSNOV58b, CG93387-01SEQ ID NO:11454401 bpDNA SequenceORF Start: ATG at 187ORF Stop: TAA at 4051GGTGCCGAGCACTCCGGACTCTACGTGAACAACAACGGGATCATCTCCTTCCTGAAGGAGGTTTCTCAGTTCACCCCAGTGGCCTTCCCCATTGCCAAGGACCGCTGCGTGGTGGCAGCCTTCTGGGCAGATGTGCACAACCGGCGTGCAGGCGACGTGTACTACCGGGAGGCCACCGACCCAGCCATGCTGCGCCGAGCCACGGAGGACGTCAGGCACTACTTCCCCGAGCTCCTGGACTTCAATGCCACCTGGGTTTTTGTTGCCACCTGGTACCGAGTGACCTTCTTTGGAGGCAGTTCCTCATCCCCTGTCAACACATTCCAGACTGTGCTCATCACAGACGGCAAGCTCTCCTTCACCATCTTCAACTATGAGTCCATCGTGTGGACCACAGGCACACACGCCAGCAGCGGGGGCAACGCCACTGGCCTCGGGGGCATCGCAGCCCAGGCTGGCTTCAACGCAGGCGATGGGCAGCGTTACTTCAGTATCCCCGGCTCGCGCACAGCAGACATGGCCGAGGTGGAGACCACCACCAACGTGGGTGTGCCCGGGCGCTGGGCGTTCAGAATCGATGATGCCCAGGTGCGCGTGGGGGGCTGCGGCCATACAACGTCCGTGTGCCTGGCCCTGCGCCCCTGCCTCAACGGCGGCAAGTGCATCGACGACTGCGTCACGGGCAACCCCTCCTACACCTGCTCCTGCCTCTCGGGCTTCACGGGGCGGAGGTGCCACCTGGACGTGAACGAATGTGCCTCCCAGCCCTGTCAGAATGGTGGGACCTGTACTCACGGCATCAACAGTTTCCGCTGCCAGTGCCCGGCTGGCTTTGGGGGACCCACCTGTGAGACAGCCCAATCCCCCTGTGACACCAAAGAGTGTCAACATGGTGGCCAGTGCCAGGTGGAGAACGGCTCTGCGGTGTGTGTGTGCCAGGCCGGATACACCGGAGCAGCCTGCGAGATGGATGTGGACGACTGCAGCCCTGACCCCTGCCTGAATGGAGGCTCTTGTGTTGACCTAGTGGGGAATTACACCTGCTTGTGTGCCGAGCCCTTCAAGGGACTTCGCTGTGAGACAGGAGACCATCCAGTGCCAGACGCCTGCCTCTCGGCCCCTTGCCACAATGGGGGCACCTGTGTGGATGCGGACCAGGGCTACGTGTGCGAGTGCCCCGAAGGCTTCATGGGCCTGGACTGCAGGGAGAGAGTCCCCGATGACTGTGAGTGCCGCAACGGAGGCAGATGCCTGGGCGCCAACACCACCCTCTGCCAGTGCCCCCTGGGATTCTTTGGGCTTCTCTGTGAATTTGAAATCACAGCCATGCCCTGCAACATGAACACACAGTGCCCAGATGGGGGCTACTGCATGGAGCACGGCGGGAGCTACCTCTGCGTCTGCCACACCGACCACAATGCCAGCCACTCCCTGCCATCACCCTGCGACTCGGACCCCTGCTTCAACGGAGGCTCCTGCGATGCCCATGACGACTCCTACACCTGCGAGTGCCCGCGCGGGTTCCACGGCAAGCACTGCGAGAAAGCCCGGCCACACCTGTGCAGCTCAGGGCCCTGCCGGAACGGGGGCACGTGCAAGGAGGCGGGCGGCGAGTACCACTGCAGCTGCCCCTACCGCTTCACTGGGAGGCACTGTGAGATCGGGAAGCCAGACTCGTGTGCCTCTGGCCCCTGTCACAACGGCGGCACCTGCTTCCACTACATTGGCAAATACAAGTGTGACTGTCCCCCAGGCTTCTCCGGGCGGCACTGCGAGATAGCCCCCTCCCCCTGCTTCCGGAGCCCGTGTGTGAATGGGGGCACCTGCGAGGACCGGGACACGGATTTCTTCTGCCACTGCCAAGCAGGGTACATGGGACGCCGGTGCCAGGCAGAGGTGGACTGCGGCCCCCCGGAGGAGGTGAAGCACGCCACACTGCGCTTCAACGGCACGCGGCTGGGCGCGGTGGCCCTGTATGCATGTGACCGTGGCTACAGCCTGAGCGCCCCCAGCCGCATCCGGGTCTGCCAGCCACACGGTGTCTGGAGTGAGCCTCCCCAGTGCCTTGAAATCGATGAGTGCCGGTCTCAGCCGTGCCTGCATGGGGGCTCTTGTCAGGACCGCGTTGCTGGGTACCTGTGCCTCTGCAGCACAGGCTATGAGGGCGCCCACTGTGAGCTGGAGAGGGATGAGTGCCGAGCTCACCCGTGCAGAAATGGAGGGTCCTGCAGGAACCTCCCAGGGCTCCAGCCCCTGCCAGCATGGAGGCCGGTGTGAGAGCGGCGGCGGGGCCTACCTGTGCGTCTGCCCAGGCCTATGTCTGCCGGTGCCCTGCAGGCTTCGTTGGAGTCCACTGTGAGACAGAGGTGGACGCCTGCGAAGAGCTTCTTCGGCTACCACTGCGAGACAGTGAGTGACCCCTGCTTCTCCAGCCCCTGTGGGGGCCGTGGCTATTGCCTGGCCAGCAACGGCTCCCACAGCTGCACCTGCAAAGTGGGCTACACGGGCGAGGACTGCGCCAAAGAGCTCTTCCCACCGACGGCCCTCAAGATGGAGAGAGTGGAGGAGAGTGGGGTCTCTATCTCCTGGAACCCGCCCAATGGTCCAGCCGCCAGGCAGATGCTTGATGGCTACGCGGTCACCTACGTCTCCTCCGACGGCTCCTACCGCCGCACAGACTTTGTGGACAGGACCCGCTCCTCGCACCAGCTCCAGGCCCTGGCGGCCGGCAGGGCCTACAACATCTCCGTCTTCTCAGTGAAGCGAAACAGTAACAACAAGAATGACATCAGCAGGCCTGCCGTGCTGCTGGCCCGCACGCGACCCCGCCCTGTGGAAGGCTTCGAGGTCACCAATGTGACGGCTAGCACCATCTCAGTGCAGTGGGCCCTGCACAGGATCCGCCATGCCACCGTCAGTGGGGTCCGTGTGTCCATCCGCCACCCTGAGGCCCTCAGGGACCAGGCCACCGATGTGGACAGGAGTGTGGACAGGTTCACCTTTAGGGCCCTGCTGCCTGGGAAGAGGTACACCATCCAGCTGACCACCCTCAGTGGGCTCAGGGGAGAGGAGCACCCCACAGAGAGCCTGGCCACCGCGCCGACGCACGTGTGGACCCGGCCCCTGCCTCCAGCAAACCTGACCGCCGCCCGAGTCACTGCCACCTCTGCCCACGTGGTCTGGGATGCCCCGACTCCAGGCAGCTTGCTGGAGGCTTATGTCATCAATGTGACCACCAGCCAGAGCACCAAGAGCCGCTATGTCCCCAACGGGAAGCTGGCGTCCTACACGGTGCGCGACCTGCTGCCGGGACGGCGGTACCAGCCCTCTGTGATAGCAGTGCAGAGCACGGAGCTCGGGCCGCAGCACAGCGAGCCCGCCCACCTCTACATCATCACCTCCCCCAGGGATGGCGCTGACAGACGCTGGCACCAGGGAGGACACCACCCTCGGGTGCTCAAGAACAGACCGCCCCCGGCGCGCCTGCCGGAGCTGCGCCTGCTCAATGACCACAGCGCCCCCGAGACCCCCACCCAGCCCCCCAGGTTCTCGGAGTTTGTGGACGGCAGAGGAAGAGTGAGCGCCAGGTTCGGTGGCTCACCCAGCAAAGCAGCCACCGTGAGATCACAACCCACAGCCTCGGCGCAGCTCGAGAACATGGAGGAAGCCCCCAAGCGGGTCAGCCCGGCCCTCCAGCTCCCTGAACACGGCAGCAAGGACATCGGAAACGTCCCTGGCAACTGTTCAGAAAACCCCTGTCAGAACGGAGGCACTTGTGTGCCGGGCGCAGACGCCCACAGCTGTGACTGCGGGCCAGGGTTCAAAGGCAGACGCTGCGAGCTCGCCTGTATAAAGGTGTCCCGCCCCTGCACAAGGCTGTTCTCCGAGACAAAGGCCTTTCCAGTCTGGGAGGGAGGCGTCTGTCACCACGTGTATAAAAGAGTCTACCGAGTTCACCAAGACATCTGCTTCAAAGAGAGCTGTGAAAGCACAAGCCTCAAGAAGACCCCAAACAGGAAACAAAGTAAGAGTCAGACACTGGAGAAATCTTAAGAAAGAAGGAACAGGCAATGTAGAGAAGCTGTCAAATGGTGGACTCCCAAACCGTTCCACCACTGCCTCAAAAAACATCTTGACCAGCAGAAGGTGGAGCTCAATGAAGGGTCAAGAGCTCAGCGAAGGGTAACTAGGTGGAACTGAGAGAAACCACGTTCACAAACTGCGTAATGCGGACTTCCTGCCGCCCTGGAGACCCCTCAACTCTCTGTCCATGTAAGGCCCTTAAAGAGATTCATAGGAACTTTGAGCATCCTTNAGATGTGAATATTGTTGGGGGCAGGATTGGGGGATAAATAGAAGGGAAGGCCACTCCACGAGTATCCCATGAACCTGGCCAGATCTNOV58b, CG93387-01SEQ ID NO:11461288 aaMW at 138908.1 kDProtein SequenceMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTIFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGDHPVPDACLSAPCHNGGTCVDADQGYVCECPEGFMGLDCRERVPDDCECRNGGRCLGANTTLCQCPLGFFGLLCEFEITAMPCNMNTQCPDGGYCMEHGGSYLCVCHTDHNASHSLPSPCDSDPCFNGGSCDAHDDSYTCECPRGFHGKHCEKARPHLCSSGPCRNGGTCKEAGGEYHCSCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCVNGGTCEDRDTDFFCHCQAGYMGRRCQAEVDCGPPEEVKHATLRFNGTRLGAVALYACDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYEGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQHSEPAHLYIITSPRDGADRRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPSKAATVRSQPTASAQLENMEEAPKRVSPALQLPEHGSKDIGNVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLEKSNOV58c, CG93387-02SEQ ID NO:11474413 bpDNA SequenceORF Start: ATG at 181ORF Stop: TAG at 4405GAGCACTCCGGACTCTACGTGAACAACAACGGGATCATCTCCTTCCTGAAGGAGGTTTCTCAGTTCACCCCAGTGGCCTTCCCCATTGCCAAGGACCGCTGCGTGGTGGCAGCCTTCTGGGCAGATGTGGACAACCGGCGTGCAGGCGACGTGTACTACCGGGAGGCCACCGACCCAGCCATGCTGCGCCGAGCCACGGAGGACGTCAGGCACTACTTCCCCGAGCTCCTGGACTTCAATGCCACCTGGGTTTTTGTTGCCACCTGGTACCGAGTGACCTTCTTTGGAGGCAGTTCCTCATCCCCTGTCAACACATTCCAGACTGTGCTCATCACAGACGGCAAGCTCTCCTTCACCATCTTCAACTATGAGTCCATCGTGTGGACCACAGGCACACACGCCAGCAGCGGGGGCAACGCCACTGGCCTCGGGGGCATCGCAGCCCAGGCTGGCTTCAACGCAGGCGATGGGCAGCGTTACTTCAGTATCCCCGGCTCGCGCACAGCAGACATGGCCGAGGTGGAGACCACCACCATCGTGGTTGTGCCCGGGCGCTGGGCGTTCATAATCGATGATGCCCAGGTGCGCGTGGGGGGCTGCGGCCATACAACGTCCGTGTGCCTGGCCCTGCGCCCCTGCCTCAACGGCGGCAAGTGCATCGACGACTGCGTCACGGGCAACCCCTCCTACACCTGCTCCTGCCTCTCGGGCTTCACGGGGCGGAGGTGCCACCTGGACGTGAACGAATGTGCCTCCCAGCCCTGTCAGAATGGTGGGACCTGTACTCACGGCATCAACAGTTTCCGCTGCCAGTGCCCGGCTGGCTTTGGGGGACCCACCTGTGAGACAGCCCAATCCCCCTGTGACACCAAAGAGTGTCAACATGGTGGCCAGTGCCAGGTGGAGAATGGCTCTGCGGTGTGTGTGTGCCAGGCCGGATACACCGGAGCAGCCTGCGAGATGGATGTGGACGACTGCAGCCCTGACCCCTGCCTGAATGGAGGCTCTTGTGTTGACCTAGTGGGGAATTACACCTGCTTGTGTGCCGAGCCCTTCAAGGGACTTCGCTGTGAGACAGGAGACCATCNNCAGTGCCAGACGCCTGCCTCTCGGCCCCTTGCCACAATGGGGGCACCTGTGTGGATGCGGACCAGGGCTACGTGTGCGAGTGCCCCGAAGGCTTCATGGGCCTGGACTGCAGGGAGAGAGTCCCCGATGACTGTGAGTGCCGCAACGGAGGCAGATGCCTGGGCGCCAACACCACCCTCTGCCCAGTGCCCCCTGGGATTCTTTGGGCTTCTCTGTGAATTTGAAATCACAGCCATGCCCTGCAACATGAACACACAGTGCCCAGATGGGGGCTACTGCATGGAGCACGGCGGGAGCTACCTCTGCGTCTGCCACACCGACCACAATGCCAGCCACTCCCTGCCATCACCCTGCGACTCGGACCCCTGCTTCAACGGAGGCTCCTGCGATGCCCATGACGACTCCTACACCTGCGAGTGCCCGCGCGGGTTCCACGGCAAGCACTGCGAGAAAGCCCGGCCACACCTGTGCAGCTCAGGGCCCTGCCGGAACGGGGGCACGTGCAAGGAGGCGGGCGGCGACTACCACTGCAGCTGCCCCTACCGCTTCACTGGGAGGCACTGTGAGATCGGGAAGCCAGACTCGTGTGCCTCTGGCCCCTGTCACAACGGCGGCACCTGCTTCCACTACATTGGCAAATACAAGTGTGACTGTCCCCCAGGCTTCTCCGGGCGGCACTGCGAGATAGCCCCCTCCCCCTGCTTCCGGAGCCCGTGTGTGAATGGGGGCACCTGCGAGGACCGGGACACGGATTTCTTCTGCCACTGCCAAGCAGGGTACATGGGACGCCGGTGCCAGGCAGAGGTGGACTGCGGCCCCCCGGAGGAGGTGAAGCACGCCACACTGCGCTTCAACGGCACGCGGCTGGGCGCGGTGGCCCTGTATGCATGTGACCGTGGCTACAGCCTGAGCGCCCCCAGCCGCATCCGGGTCTGCCAGCCACACGGTGTCTGGAAAATCGATGAGTGCCGGTCTCAGCCGTGCCTGCATGGGGGCTCTTGTCAGGACCGCGTTGCTGGGTACCTGTGCCTCTGCAGCACAGGCTATGAGGGCGCCCACTGTGAGCTGGAGAGGGATGAGTGCCGAGCTCACCCGTGCAGAAATGGAGGGTCCTGCAGGAACCTCCCAGGGGCCTATGTCTGCCGGTGCCCTGCAGGCTTCGTTGGAGTCCACTGTGAGACAGAGGTGGACGCCTGCGACTCCAGCCCCTGCCAGCATGGAGGCCGGTGTGAGAGCGGCGGCGGGGCCTACCTGTGCGTCTGCCCAGAGAGCTTCTTCGGCTACCACTGCGAGACAGTGAGTGACCCCTGCTTCTCCAGCCCCTGTGGGGGCCGTGGCTATTGCCTGGCCAGCAACGGCTCCCACAGCTGCACCTGCAAAGTGGGCTACACGGGCGAGGACTGCGCCAAAGAGCTCTTCCCACCGACGGCCCTCAAGATGGAGAGAGTGGAGGAGAGTGGGGTCTCTATCTCCTGGAACCCGCCCAATGGTCCAGCCGCCAGGCAGATGCTTGATGGCTACGCGGTCACCTACGTCTCCTCCGACGGCTCCTACCGCCGCACAGACTTTGTGGACAGGACCCGCTCCTCGCACCAGCTCCAGGCCCTGGCGGCCGGCAGGGCCTACAACATCTCCGTCTTCTCAGTGAAGCGAAACAGTAACAACAAGAATGACATCAGCAGGCCTGCCGTGCTGCTGGCCCGCACGCGACCCCGCCCTGTGGAAGGCTTCGAGGTCACCAATGTGACGGCTAGCACCATCTCAGTGCAGTGGGCCCTGCACAGGATCCGCCATGCCACCGTCAGTGGGGTCCGTGTGTCCATCCGCCACCCTGAGGCCCTCAGGGACCAGGCCACCGATGTGGACAGGAGTGTGGACAGGTTCACCTTTAGGGCCCTGCTGCCTGGGAAGAGGTACACCATCCAGCTGACCACCCTCAGTGGGCTCAGGGGAGAGGAGCACCCCACAGAGAGCCTGGCCACCGCGCCGACGCACGTGTGGACCCGGCCCCTGCCTCCAGCAAACCTGACCGCCGCCCGAGTCACTGCCACCTCTGCCCACGTGGTCTGGGATGCCCCGACTCCAGGCAGCTTGCTGGAGGCTTATGTCATCAATGTGACCACCAGCCAGAGCACCAAGAGCCGCTATGTCCCCAACGGGAAGCTGGCGTCCTACACGGTGCGCGACCTGCTGCCGGGACGGCGGTACCAGCTCTCTGTGATAGCAGTGCAGAGCACGGAGCTCGGGCCGCAGCACAGCGAGCCCGCCCACCTCTACATCATCACCTCCCCCAGGGATGGCGCTGACAGACGCTGGCACCAGGGAGGACACCACCCTCGGGTGCTCAAGAACAGACCGCCCCCGGCGCGCCTGCCGGAGCTGCGCCTGCTCAATGACCACAGCGCCCCCGAGACCCCCACCCAGCCCCCCAGGTTCTCGGAGCTTGTGGACGGCAGAGGAAGAGTGAGCGCCAGGTTCGGTGGCTCACCCAGCAAAGCAGCCACCGTGAGATCACGTCCTGTCCCCTACATGATGAGCCCACCCCCACCGCCAGCGCAGTCTCCAGCCAGTGACCCCCACCCCGACTGTGCACAAGGCGCGGGGCTCGTGGGCCGCCGGCAGCATGCACCTCCATGGCAGGAGGGGCAGCTCGGACATCCGTGCTCCCTGAGATATAGAAGCACTCAAAAGGGTGGCCCCAGGACCATCCCGGGTGCAAAGCAGCTGCGCCGTGTGGTCACCGCCTGGCTTCTCCTAGAACCCACAGCCTCGGCGCAGCTCGAGAACATGGAGGAAGCCCCCAAGCGGGTCAGCCTGGCCCTCCAGCTCCCTGAACACGGCAGCAAGGACATCGGAAGTTATGCAGGACCTGAACTGTCTCCTAGTCCGGGGCTCTGCCTCGTGAGGATCGAGGCCAGCACGTCCCTGCAGGGCACCAAGCATCTGCTGAGCACCTGCAGCACACAAGCAAAGGAGCAGGGTGGAGCCTTCACGCTGCCGTGCCTGTGTGGACCAGTCCAGGGTGACCACGGGGTAGGTGAGGGAAAGCCTGTCTTCACAGACCACTCTCCAGCTGACGTCCCTGGCAACTGTTCAGAAAACCCCTGTCAGAACGGAGGCACTTGTGTGCCGGGCGCAGACGCCCACAGCTGTGACTGCGGGCCAGGGTTCAAAGGCAGACGCTGCGAGCTCGGTATAAAAGAGTCTACCGAGTTCACCAAGACATCTGCTTCAAAGAGAGCTGTGAAAGCACAAGCCTCAAGAAGACCCCAAACAGGTGCCTCTGGGGAGCAGGCCCATGCCGTGTCCTGCATGTAGNNNNNNNOV58c, CG93387-02SEQ ID NO:11481408 aaMW at 150587.4 kDProtein SequenceMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTIFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTIVVVPGRWAFIIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVFNYTCLCAEPFKGLRCETGDHXQCQTPASRPLATMGAPVWMRTRATCASAPKASWAWTAGRESPMTVSAATEADAWAPTPPSAWCPLGFFGLLCEFEITAMPCNMNTWCPDGGYCMEHGGSYLCVCHTDHNASHSLPSPCDSDPCFNGGSCDAHDDSYTCECPRGFHGKHCEKARPHLCSSGPCRNGGTCKEAGGEYHCSCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCVNGGTCEDRDTDFFCHCQAGYMGRRCQAEVDCGPPEEVKHATLRFNGTRLGAVALYACDRGYSLSAPSRIRVCQPHGVWKIDECRSQPCLHGGSCQDRVAGYLCLCSTGYEGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSHQLQALAAGPAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQSTKSRYVPNGKLASYTVRDLLPGRRYQLSVIAVQSTELGPQHSEPAHLYIITSPRDGADRRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSELVDGRGRVSARFGGSPSKAATVRSRPVPYMMSPPPPPAQSPASDPHPDCAQGAGLVGRRQHAPPWQEGQLGHPCSLRYRSTQKGGPRTIPGAKQLRRVVTAWLLLEPTASAQLENMEEAPKRVSLALQLPEHGSKDIGSYAGPELSPSPGLCLVRIEASTSLQGTKHLLSTCSTQAKEQGGAFTLPCLCGPVQGDHGVGEGKPVFTDHSPADVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELGIKESTEFTKTSASKRAVKAQASRPPQTGASGEQAHAVSCMNOV58d, CG93387-03SEQ ID NO:11492877 bpDNA SequenceORF Start: ATG at 6ORF Stop: TAA at 2868GCTTCATGGGCCTGGACTGCAGGGAGAGAGTCCCCGATGACTGTGAGTGCCGCAACGGAGGCAGATGCCTGGGCGCCAACACCACCCTCTGCCAGTGCCCCCTGGGATTCTTTGGGCTTCTCTGTGAATTTGAAATCACAGCCATGCCCTGCAACATGAACACACAGTGCCCAGATGGGGGCTACTGCATGGAGCACGGCGGGAGCTACCTCTGCGTCTGCCACACCGACCACAATGCCAGCCACTCCCTGCCATCACCCTGCGACTCGGACCCCTGCTTCAACGGAGGCTCCTGCGATGCCCATGACGACTCCTACACCTGCGAGTGCCCGCGCGGGTTCCACGGCAAGCACTGCGAGAAAGCCCGGCCACACCTGTGCAGCTCAGGGCCCTGCCGGAACGGGGGCACGTGCAAGGAGGCGGGCGGCGAGTACCACTGCAGCTGCCCCTACCGCTTCACTGGGAGGCACTGTGAGATCGGGAAGCCAGACTCGTGTGCCTCTGGCCCCTGTCACAACGGCGGCACCTGCTTCCACTACATTGGCAAATACAAGTGTGACTGTCCCCCAGGCTTCTCCGGGCGGCACTGCGAGATAGCCCCCTCCCCCTGCTTCCGGAGCCCGTGTGTGAATGGGGGCACCTGCGAGGACCGGGACACGGATTTCTTCTGCCACTGCCAAGCAGGGTACATGGGACGCCGGTGCCAGGCAGAGGTGGACTGCGGCCCCCCGGAGGAGGTGAAGCACGCCACACTGCGCTTCAACGGCACGCGGCTGGGCGCGGTGGCCCTGTATGCATGTGACCGTGGCTACAGCCTGAGCGCCCCCAGCCGCATCCGGGTCTGCCAGCCACACGGTGTCTGGAGTGAGCCTCCCCAGTGCCTTGAAATCGATGAGTGCCGGTCTCAGCCGTGCCTGCATGGGGGCTCTTGTCAGGACCGCGTTGCTGGGTACCTGTGCCTCTGCAGCACAGGCTATGAGGGCGCCCACTGTGAGCTGGAGAGGGATGAGTGCCGAGCTCACCCGTGCAGAAATGGAGGGTCCTGCAGGAACCTCCCAGGGGCCTATGTCTGCCGGTGCCCTGCAGGCTTCGTTGGAGTCCACTGTGAGACAGAGGTGGACGCCTGCGACTCCAGCCCCTGCCAGCATGGAGGCCGGTGTGAGAGCGGCGGCGGGGCCTACCTGTGCGTCTGCCCAGAGAGCTTCTTCGGCTACCACTGCGAGACAGTGAGTGACCCCTGCTTCTCCAGCCCCTGTGGGGGCCGTGGCTATTGCCTGGCCAGCAACGGCTCCCACAGCTGCACCTGCAAAGTGGGCTACACGGGCGAGGACTGCGCCAAAGAGCTCTTCCCACCGACGGCCCTCAAGATGGAGAGAGTGGAGGAGAGTGGGGTCTCTATCTCCTGGAACCCGCCCAATGGTCCAGCCGCCAGGCAGATGCTTGATGGCTACGCGGTCACCTACGTCTCCTCCGACGGCTCCTACCGCCGCACAGACTTTGTGGACAGGACCCGCTCCTCGCACCAGCTCCAGGCCCTGGCGGCCGGCAGGGCCTACAACATCTCCGTCTTCTCAGTGAAGCGAAACAGTAACAACAAGAATGACATCAGCAGGCCTGCCGTGCTGCTGGCCCGCACGCGACCCCGCCCTGTGGAAGGCTTCGAGGTCACCAATGTGACGGCTAGCACCATCTCAGTGCAGTGGGCCCTGCACAGGATCCGCCATGCCACCGTCAGTGGGGTCCGTGTGTCCATCCGCCACCCTGAGGCCCTCAGGGACCAGGCCACCGATGTGGACAGGAGTGTGGACAGGTTCACCTTTAGGGCCCTGCTGCCTGGGAAGAGGTACACCATCCAGCTGACCACCCTCAGTGGGCTCAGGGGAGAGGAGCACCCCACAGAGAGCCTGGCCACCGCGCCGACGCACGTGTGGACCCGGCCCCTGCCTCCAGCAAACCTGACCGCCGCCCGAGTCACTGCCACCTCTGCCCACGTGGTCTGGGATGCCCCGACTCCAGGCAGCTTGCTGGAGGCTTATGTCATCAATGTGACCACCAGCCAGAGCACCAAGAGCCGCTATGTCCCCAACGGGAAGCTGGCGTCCTACACGGTGCGCGACCTGCTGCCGGGACGGCGGTACCAGCCCTCTGTGATAGCAGTGCAGAGCACGGAGCTCGGGCCGCAGCACAGCGAGCCCGCCCACCTCTACATCATCACCTCCCCCAGGGATGGCGCTGACAGACGCTGGCACCAGGGAGGACACCACCCTCGGGTGCTCAAGAACAGACCGCCCCCGGCGCGCCTGCCGGAGCTGCGCCTGCTCAATGACCACAGCGCCCCCGAGACCCCCACCCAGCCCCCCAGGTTCTCGGAGTTTGTGGACGGCAGAGGAAGAGTGAGCGCCAGGTTCGGTGGCTCACCCAGCAAAGCAGCCACCGTGAGATCACAACCCACAGCCTCGGCGCAGCTCGAGAACATGGAGGAAGCCCCCAAGCGGGTCAGCCCGGCCCTCCAGCTCCCTGAACACGGCAGCAAGGACATCGGAAACGTCCCTGGCAACTGTTCAGAAAACCCCTGTCAGAACGGAGGCACTTGTGTGCCGGGCGCAGACGCCCACAGCTGTGACTGCGGGCCAGGGTTCAAAGGCAGACGCTGCGAGCTCGCCTGTATAAAGGTGTCCCGCCCCTGCACAAGGCTGTTCTCCGAGACAAAGGCCTTTCCAGTCTGGGAGGGAGGCGTCTGTCACCACGTGTATAAAAGAGTCTACCGAGTTCACCAAGACATCTGCTTCAAAGAGAGCTGTGAAAGCACAAGCCTCAAGAAGACCCCAAACAGGAAACAAAGTAAGAGTCAGACACTGGAGAAATCTTAAGAAAGAANOV58d, CG93387-03SEQ ID NO:1150954 aaMW at 103791.4 kDProtein SequenceMGLDCRERVPDDCECRNGGRCLGANTTLCQCPLGFFGLLCEFEITAMPCNNNTQCPDGGYCMEHGGSYLCVCHTDHNASHSLPSPCDSDPCFNGGSCDAHDDSYTCECPRGFHGKHCEKARPHLCSSGPCRNGGTCKEAGGEYHCSCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCVNGGTCEDRDTDFFCHCQAGYMGRRCQAEVDCGPPEEVKHATLRFNGTRLGAVALYACDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYEGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQHSEPAHLYIITSPRDGADRRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPSKAATVRSQPTASAQLENMEEAPKRVSPALQLPEHGSKDIGNVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLEKSNOV58e, CG93387-04SEQ ID NO:1151j7459 bpDNA SequencelORF Start: ATG at 182lORF Stop: TAA at 4046GAGCACTCCGGACTCTACGTGAACAACAACGGGATCATCTCCTTCCTGAAGGAGGTTTCTCAGTTTCACCCCAGTGGCCTTCCCCATTGCCAAGGACCGCTGCGTGGTGGCAGCCTTCTGGGCAGATGTGGACAACCGGCGTGCAGGCGACGTGTACTACCGGGAGGCCACCGACCCAGCCATGCTGCGCCGAGCCACGGAGGACGTCAGGCACTACTTCCCCGAGCTCCTGGACTTCAATGCCACCTGGGTTTTTGTTGCCACCTGGTACCGAGTGACCTTCTTTGGAGGCAGTTCCTCATCCCCTGTCAACACATTCCAGACTGTGCTCATCACAGACGGCAAGCTCTCCTTCACCATCTTCAACTATGAGTCCATCGTGTGGACCACAGGCACACACGCCAGCAGCGGGGGCAACGCCACTGGCCTCGGGGGCATCGCAGCCCAGGCTGGCTTCAACGCAGGCGATGGGCAGCGTTACTTCAGTATCCCCGGCTCGCGCACAGCAGACATGGCCGAGGTGGAGACCACCACCAACGTGGGTGTGCCCGGGCGCTGGGCGTTCAGAATCGATGATGCCCAGGTGCGCGTGGGGGGCTGCGGCCATACAACGTCCGTGTGCCTGGCCCTGCGCCCCTGCCTCAACGGCGGCAAGTGCATCGACGACTGCGTCACGGGCAACCCCTCCTACACCTGCTCCTGCCTCTCGGGCTTCACGGGGCGGAGGTGCCACCTGGACGTGAACGAATGTGCCTCCCAGCCCTGTCAGAATGGTGGGACCTGTACTCACGGCATCAACAGTTTCCGCTGCCAGTGCCCGGCTGGCTTTGGGGGACCCACCTGTGAGACAGCCCAATCCCCCTGTGACACCAAAGAGTGTCAACATGGTGGCCAGTGCCAGGTGGAGAATGGCTCTGCGGTGTGTGTGTGCCAGGCCGGATACACCGGAGCAGCCTGCGAGATGGATGTGGACGACTGCAGCCCTGACCCCTGCCTGAATGGAGGCTCTTGTGTTGACCTAGTGGGGAATTACACCTGCTTGTGTGCCGAGCCCTTCAAGGGACTTCGCTGTGAGACAGGAGACCATCCAGTGCCAGACGCCTGCCTCTCGGCCCCTTGCCACAATGGGGGCACCTGTGTGGATGCGGACCAGGGCTACGTGTGCGAGTGCCCCGAAGGCTTCATGGGCCTGGACTGCAGGGAGAGAGTCCCCGATGACTGTGAGTGCCGCAACGGAGGCAGATGCCTGGGCGCCAACACCACCCTCTGCCAGTGCCCCCTGGGATTCTTTGGGCTTCTCTGTGAATTTGAAATCACAGCCATGCCCTGCAACATGAACACACAGTGCCCAGATGGGGGCTACTGCATGGAGCACGGCGGGAGCTACCTCTGCGTCTGCCACACCGACCACAATGCCAGCCACTCCCTGCCATCACCCTGCGACTCGGACCCCTGCTTCAACGGAGGCTCCTGCGATGCCCATGACGACTCCTACACCTGCGAGTGCCCGCGCGGGTTCCACGGCAAGCACTGCGAGAAAGCCCGGCCACACCTGTGCAGCTCAGGGCCCTGCCGGAACGGGGGCACGTGCAAGGAGGCGGGCGGCGAGTACCACTGCAGCTGCCCCTACCGCTTCACTGGGAGGCACTGTGAGATCGGGAAGCCAGACTCGTGTGCCTCTGGCCCCTGTCACAACGGCGGCACCTGCTTCCACTACATTGGCAAATACAAGTGTGACTGTCCCCCAGGCTTCTCCGGGCGGCACTGCGAGATAGCCCCCTCCCCCTGCTTCCGGAGCCCGTGTGTGAATGGGGGCACCTGCGAGGACCGGGACACGGATTTCTTCTGCCACTGCCAAGCAGGGTACATGGGACGCCGGTGCCAGGCAGAGGTGGACTGCGGCCCCCCGGAGGAGGTGAAGCACGCCACACTGCGCTTCAACGGCACGCGGCTGGGCGCGGTGGCCCTGTATGCATGTGACCGTGGCTACAGCCTGAGCGCCCCCAGCCGCATCCGGGTCTGCCAGCCACACGGTGTCTGGAGTGAGCCTCCCCAGTGCCTTGAAATCGATGAGTGCCGGTCTCAGCCGTGCCTGCATGGGGGCTCTTGTCAGGACCGCGTTGCTGGGTACCTGTGCCTCTGCAGCACAGGCTATGAGGGCGCCCACTGTGAGCTGGAGAGGGATGAGTGCCGAGCTCACCCGTGCAGAAATGGAGGGTCCTGCAGGAACCTCCCAGGGGCCTATGTCTGCCGGTGCCCTGCAGGCTTCGTTGGAGTCCACTGTGAGACAGAGGTGGACGCCTGCGACTCCAGCCCCTGCCAGCATGGAGGCCGGTGTGAGAGCGGCGGCGGGGCCTACCTGTGCGTCTGCCCAGAGAGCTTCTTCGGCTACCACTGCGAGACAGTGAGTGACCCCTGCTTCTCCAGCCCCTGTGGGGGCCGTGGCTATTGCCTGGCCAGCAACGGCTCCCACAGCTGCACCTGCAAAGTGGGCTACACGGGCGAGGACTGCGCCAAAGAGCTCTTCCCACCGACGGCCCTCAAGATGGAGAGAGTGGAGGAGAGTGGGGTCTCTATCTCCTGGAACCCGCCCAATGGTCCAGCCGCCAGGCAGATGCTTGATGGCTACGCGGTCACCTACGTCTCCTCCGACGGCTCCTACCGCCGCACAGACTTTGTGGACAGGACCCGCTCCTCGCACCAGCTCCAGGCCCTGGCGGCCGGCAGGGCCTACAACATCTCCGTCTTCTCAGTGAAGCGAAACAGTAACAACAAGAATGACATCAGCAGGCCTGCCGTGCTGCTGGCCCGCACGCGACCCCGCCCTGTGGAAGGCTTCGAGGTCACCAATGTGACGGCTAGCACCATCTCAGTGCAGTGGGCCCTGCACAGGATCCGCCATGCCACCGTCAGTGGGGTCCGTGTGTCCATCCGCCACCCTGAGGCCCTCAGGGACCAGGCCACCGATGTGGACAGGAGTGTGGACAGGTTCACCTTTAGGGCCCTGCTGCCTGGGAAGAGGTACACCATCCAGCTGACCACCCTCAGTGGGCTCAGGGGAGAGGAGCACCCCACAGAGAGCCTGGCCACCGCGCCGACGCACGTGTGGACCCGGCCCCTGCCTCCAGCAAACCTGACCGCCGCCCGAGTCACTGCCACCTCTGCCCACGTGGTCTGGGATGCCCCGACTCCAGGCAGCTTGCTGGAGGCTTATGTCATCAATGTGACCACCAGCCAGAGCACCAAGAGCCGCTATGTCCCCAACGGGAAGCTGGCGTCCTACACGGTGCGCGACCTGCTGCCGGGACGGCGGTACCAGCCCTCTGTGATAGCAGTGCAGAGCACGGAGCTCGGGCCGCAGCACAGCGAGCCCGCCCACCTCTACATCATCACCTCCCCCAGGGATGGCGCTGACAGACGCTGGCACCAGGGAGGACACCACCCTCGGGTGCTCAAGAACAGACCGCCCCCGGCGCGCCTGCCGGAGCTGCGCCTGCTCAATGACCACAGCGCCCCCGAGACCCCCACCCAGCCCCCCAGGTTCTCGGAGTTTGTGGACGGCAGAGGAAGAGTGAGCGCCAGGTTCGGTGGCTCACCCAGCAAAGCAGCCACCGTGAGATCACAACCCACAGCCTCGGCGCAGCTCGAGAACATGGAGGAAGCCCCCAAGCGGGTCAGCCCGGCCCTCCAGCTCCCTGAACACGGCAGCAAGGACGTCGGAAACGTCCCTGGCAACTGTTCAGAAAACCCCTGTCAGAACGGAGGCACTTGTGTGCCGGGCGCAGACGCCCACAGCTGTGGCTGCGGGCCAGGGTTCAAAGGCAGACGCTGCGAGCTCGCCTGTATAAAGGTGTCCCGCCCCTGCACAAGGCTGTTCTCCGAGACAAAGGCCTTTCCAGTCTGGGAGGGAGGCGTCTGTCACCACGTGTATAAAAGAGTCTACCGAGTTCACCAAGACATCTGCTTCAAAGAGAGCTGTGAAAGCACAAGCCTCAAGAAGACCCCAAACAGGAAACAAAGTAAGAGTCAGACACTGGAGAAATCTTAAGGATTTAAGACGTTCTTGTTACACTCCACCAACCTCACGAGTTTCTAACACCCAGGAACATGACCTCTAAAAACTGGATGAAAAAGGACACCCTGAGAAAAGGTCCTAGCTGGAGTCAGTCCCCTCTGTGACCTCTCTCCTCAGGCCTCTAGAGGACAGATGGCCAGGCCTGTGCACACACCAGCCCACCCTGAGAGACCCCTCTGGGACCAACCACCTGTGAGTCCTGCGATGCCTTTAAGCAGCCTGTGCCCTCACCCAAGCTGCAGTTCCTGAAGGTGTAGTCTGTGTCTCTGCGGATGACATGACAGCTCGCCATTCCCCGGAATCAGTGAGGCTGTCAGTCAGCCACGCTTCTGCAGTATGCAGAAACCTGTTCTTAGACTCCAAAGCCAGAGAAAGAATTCTCCCTTCGAGGCCCAACAAATTGAGAAGGAACTGTGATGGACCACTTCCAAAACAGAGACGGGGGCAGGGGCTGAAGGGCAGAGACCAGGTGATGTCAGAAGGAAAGCCGGGTTGCAGACACAGCCGCCCCTGCTCTGGTCCTCCAGCGTGTTTATGACGCTCGTGCAGGTCGACGAGCCATCCTATGGACTAGTTAACACTAAGGTGGAGTTCAGACTTTTTTAGACAACGGCGCCACTGGCAGCCTTTCTCTATCAAGGGTCAGACGGTAAACGTTTTCAGCTTTGCAGACCAGAGGTCCCTCTGGCTACAGTAGCGCAGACACAGCCACAGGCATGTCATTGAATGGCTGCGGCTATGTTCCAATAAAAACTTATTTACAATAACAGGTGGTGGCCAAATTGGCCCATGGGCCTTATTTGGTGAACCCTGTTCTATGAGATCACCTAGGCTTCAGCCTTAAACAGTGGAAGCCATCCCCTGAATGACAAGTCACAAGGGTATCAAAGAAAGACCCCTGAATTTTCATGGAAAAAGCTATTCAGACCCCTGCTTGGAAAGCTAAGGCACACTGCCACGAAGCAGCAAGGACGCCTTACAAGTCTCAGTGCAACAGAGATGGACACCTGGGCTGGGCTGGACAATGTTTAAGGTTCCTTTTAGTCCATGACTCAAGTGATACTGTTTTAGGCTATCAGGTAGTAAACACGATCTTAGACATCCCCATCTTTGTAAGCAGAACAGTACGGCACTTCACCACATCTGCTTCCCACCATGCTTCTAAGCAGCTGTCTTCCCCCTGCTAATGTTACAACCAAAGCAGCCACCCCACCTCCTCTCGTGTTGAGCCTCACGACCGCTGACCCAGCTGGAAAGCCAGCGCCCTGCCGCGTCACCCTGACTCTGCTCAGAGCCAGCATTCCAGCCACAAAGAGGGCCTCCTTCCTTTCCTCTTTCATAAAAATGTTTTTTGAAGAGTTAGAGTATATTTTAGGCTTTTTATCTTTATTAAAATTTCATGTGCATGTGTCTGTGTATTCTGCAATTTGTCATTTTCAGAAAGAAGGAACAGGCAATTCGAGAAGTTTCACCTGTACTCCCGAGCTGTTCCCCAGGCTCCAGACCCACTTGAGAGCAGAAGGTGGAGCTCAATGAAGGGTCTCGAGCTCAGCGAAGGGTCACTGGGTGAACTGAGAGAAACCACGTTCACAAACGCGTACTGCGGACTTCCTGCCGCCCTGGGACCTGTCACTGTTTGTCCATGTAAGCTACAGCATTACTAGCAGATGCTAAGATCGAGTGATATCACTGGAAAAGTAGGTGAATCCTACTAGGAAACTTTCTACTCCCTACTAGGACCTCAAGCCCCTCAGCCACACAGCAAATGCTAATATGCTCCAGTGTTAGCTTAGAAGCCTTGTGTCAACAAGAACTGGCTCCTGAGTCCCAAGCTTGGTGCCACACAGCAAATGCTAATATGCTCCAGTGTTAGCTTAGAAGCCTTGTGTCAACAAGAACTGGCTCCTGAGTCCCAAGCTTGGTGCCACACAGCAAATGCTAATATGCTCCAGTGTTAGCTTAGAAGCCTTGTGTCAACAAGAACTGGCTCCTGAGTCCCAAGTGCTGTCACAGGACTTGCCCATTGGGATGTTTTCCACATTAAATATCAAGTAAAAAGACTTCCTGGTGCTCAGGAATTACAGTTCGTTCTTGAAACATTCCAAAGAGGCCACCACAGCTTTTCCCATGTGGCTTCTTTTAAAAACTCAAATGGCTTCCTTGAAAATACTCAAAGTCCACCCAAGGAAATTAGTAATAATAGAATCAGAAAACTGTCAGGAGCATAAAGATTTCTGTATCAAAATGAAAGAAGCAATCCTGAGTTGCTGAATTACCCATCTGCTAATGAAACCGGGATGGACTGATCATCTAACCAAGTGCAGACTGAGGATTCTACTTAGTCCTCCGACTGGGTACAACAACAGCCTAGGTTCTAGGGAGGGTGGCAGTGACCGGGATGCCACAATGGAAGAGAAAATGAAAACACTGGCACAGTGAAATGTCTCATTTCCAAACAGTTTTGCCTATGGCCAAGCAAGGCAATAAAGACAAACTCTCCCTTTTCCCCATTGCGTGTGGGCTGCCAGGTACAAGTAAGGGAATCTTTGCTGTGCCCACTGTCCTCCAGTAGAGAACCCAACAGGCAACGGCCCCACTCAGGTATCAGCTCACCTCCTGCACCTCCCCTTAGCAGGAACTCCTTCCACTGGCAAAGGACTGCCACTGCCATCTGACTCAACTGTGGCGATGTGGACGGAGTCACCGAGCTGCTTTTCTTTTGCAAAACAAAAGTCTTTTTCTTTGCAGTCACGCTGTAAGACGAGGCTGCTGGAGAAAACAAAAGCACCTAGATTTCAGTGCTGAATCCCCACAATTGCATGCAGCTCACACCTACCAGGGGTATTCCAGTGCATAGGGGAAAGGAACCCGGCTGAAAAACCAGCTCCTTATTTTTCTTTTAAATAAAATAATACGATCCTAAGTCCATTTACCATCTGAAGTTGTCACGAGTGAACAGTCACATTACTGTTGTGGACCAGGCCTTAGATGAGTTTCTCAGGCTCAGCACTGACATTCTGGGCCGGATCATCCTCTCTTGTGGGACCATCCTGTGCACTGCAGGATGTTTCACAGCACCCCTGGCCTCTACCCACTAGAATCCTACAATCTACCAGATCCTAGGATCTAGTTGATCCTAGAATGCTACCAAGGAGACTTGAATTTTGGTCCCATCATCAAAAATGCCTTCTGCATCAATCCTATGCCAGTTTCCCCTAAAAGAGGGCTAACTGGAATGTTCTAGGATGTACCAATCCTCCAGGACCCTCTTAGAGCTCATGCCATCAGAGACAGGCCTCTACCTCAGGGATCACCCCGGCTGACATCAAATTCCTCTTCTCTTTTCCCAACATTTCAAATTGTTCTTCGGACTCATTGAGTTCCNOV58e, CG93387-04SEQ ID NO:11521288 aaMW at 138836.0 kDProtein SequenceMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTIFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGDHPVPDACLSAPCHNGGTCVDADQGYVCECPEGFMGLDCRERVPDDCECRNGGRCLGANTTLCQCPLGFFGLLCEFEITAMPCNMNTQCPDGGYCMEHGGSYLCVCHTDHNASHSLPSPCDSDPCFNGGSCDAHDDSYTCECPRGFHGKHCEKARPHLCSSGPCRNGGTCKEAGGEYHCSCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCVNGGTCEDRDTDFFCHCQAGYMGRRCQAEVDCGPPEEVKHATLRFNGTRLGAVALYACDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYEGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQWALHRIREATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQHSEPAHLYIITSPRDGADRRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPSKAATVRSQPTASAQLENMEEAPKRVSPALQLPEHGSKDVGNVPGNCSENPCQNGGTCVPGADAHSCGCGPGFKGRRCELACIKVSRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLEKSNOV58f, SNP13382519 ofSEQ ID NO:11535432 bpCG93387-05, DNA SequenceORF Start: ATG at 187ORF Stop: TAA at 3991SNP Pos: 4223SNP Change: C to TGGTGCCGAGCACTCCGGACTCTACGTGAACAACAACGGGATCATCTCCTTCCTGAAGGAGGTTTCTCAGTTCACCCCAGTGGCCTTCCCCATTGCCAAGGACCGCTGCGTGGTGGCAGCCTTCTGGGCAGATGTGGACAACCGGCGTGCAGGCGACGTGTACTACCGGGAGGCCACCGACCCAGCCATGCTGCGCCGAGCCACGGAGGACGTCAGGCACTACTTCCCCGAGCTCCTGGACTTCAATGCCACCTGGGTTTTTGTTGCCACCTGGTACCGAGTGACCTTCTTTGGAGGCAGTTCCTCATCCCCTGTCAACACATTCCAGACTGTGCTCATCACAGACGGCAAGCTCTCCTTCACCATCTTCAACTATGAGTCCATCGTGTGGACCACAGGCACACACGCCAGCAGCGGGGGCAACGCCACTGGCCTCGGGGGCATCGCAGCCCAGGCTGGCTTCAACGCAGGCGATGGGCAGCGTTACTTCAGTATCCCCGGCTCGCGCACAGCAGACATGGCCGAGGTGGAGACCACCACCAACGTGGGTGTGCCCGGGCGCTGGGCGTTCAGAATCGATGATGCCCAGGTGCGCGTGGGGGGCTGCGGCCATACAACGTCCGTGTGCCTGGCCCTGCGCCCCTGCCTCAACGGCGGCAAGTGCATCGACGACTGCGTCACGGGCAACCCCTCCTACACCTGCTCCTGCCTCTCGGGCTTCACGGGGCGGAGGTGCCACCTGGACGTGAACGAATGTGCCTCCCAGCCCTGTCAGAATGGTGGGACCTGTACTCACGGCATCAACAGTTTCCGCTGCCAGTGCCCGGCTGGCTTTGGGGGACCCACCTGTGAGACAGCCCAATCCCCCTGTGACACCAAAGAGTGTCAACATGGTGGCCAGTGCCAGGTGGAGAACGGCTCTGCGGTGTGTGTGTGCCAGGCCGGATACACCGGAGCAGCCTGCGAGATGGATGTGGACGACTGCAGCCCTGACCCCTGCCTGAATGGAGGCTCTTGTGTTGACCTAGTGGGGAATTACACCTGCTTGTGTGCCGAGCCCTTCAAGGGACTTCGCTGTGAGACAGGAGACCATCCAGTGCCAGACGCCTGCCTCTCGGCCCCTTGCCACAATGGGGGCACCTGTGTGGATGCGGACCAGGGCTACGTGTGCGAGTGCCCCGAAGGCTTCATGGGCCTGGACTGCAGGGAGAGAGTCCCCGATGACTGTGAGTGCCGCAACGGAGGCAGATGCCTGGGCGCCAACACCACCCTCTGCCAGTGCCCCCTGGGATTCTTTGGGCTTCTCTGTGAATTTGAAATCACAGCCATGCCCTGCAACATGAACACACAGTGCCCAGATGGGGGCTACTGCATGGAGCACGGCGGGAGCTACCTCTGCGTCTGCCACACCGACCACAATGCCAGCCACTCCCTGCCATCACCCTGCGACTCGGACCCCTGCTTCAACGGAGGCTCCTGCGATGCCCATGACGACTCCTACACCTGCGAGTGCCCGCGCGGGTTCCACGGCAAGCACTGCGAGAAAGCCCGGCCACACCTGTGCAGCTCAGGGCCCTGCCGGAACGGGGGCACGTGCAAGGAGGCGGGCGGCGAGTACCACTGCAGCTGCCCCTACCGCTTCACTGGGAGGCACTGTGAGATCGGGAAGCCAGACTCGTGTGCCTCTGGCCCCTGTCACAACGGCGGCACCTGCTTCCACTACATTGGCAAATACAAGTGTGACTGTCCCCCAGGCTTCTCCGGGCGGCACTGCGAGATAGCCCCCTCCCCCTGCTTCCGGAGCCCGTGTGTGAATGGGGGCACCTGCGAGGACCGGGACACGGATTTCTTCTGCCACTGCCAAGCAGGGTACATGGGACGCCGGTGCCAGGCAGAGGTGGACTGCGGCCCCCCGGAGGAGGTGAAGCACGCCACACTGCGCTTCAACGGCACGCGGCTGGGCGCGGTGGCCCTGTATGCATGTGACCGTGGCTACAGCCTGAGCGCCCCCAGCCGCATCCGGGTCTGCCAGCCACACGGTGTCTGGAGTGAGCCTCCCCAGTGCCTTGAAATCGATGAGTGCCGGTCTCAGCCGTGCCTGCATGGGGGCTCTTGTCAGGACCGCGTTGCTGGGTACCTGTGCCTCTGCAGCACAGGCTATGAGGGCGCCCACTGTGAGCTGGAGAGGGATGAGTGCCGAGCTCACCCGTGCAGAAATGGAGGGTCCTGCAGGAACCTCCCAGGGGCCTATGTCTGCCGGTGCCCTGCAGGCTTCGTTGGAGTCCACTGTGAGACAGAGGTGGACGCCTGCGACTCCAGCCCCTGCCAGCATGGAGGCCGGTGTGAGAGCGGCGGCGGGGCCTACCTGTGCGTCTGCCCAGAGAGCTTCTTCGGCTACCACTGCGAGACAGTGAGTGACCCCTGCTTCTCCAGCCCCTGTGGGGGCCGTGGCTATTGCCTGGCCAGCAACGGCTCCCACAGCTGCACCTGCAAAGTGGGCTACACGGGCGAGGACTGCGCCAAAGAGCTCTTCCCACCGACGGCCCTCAAGATGGAGAGAGTGGAGGAGAGTGGGGTCTCTATCTCCTGGAACCCGCCCAATGGTCCAGCCGCCAGGCAGATGCTTGATGGCTACGCGGTCACCTACGTCTCCTCCGACGGCTCCTACCGCCGCACAGACTTTGTGGACAGGACCCGCTCCTCGCACCAGCTCCAGGCCCTGGCGGCCGGCAGGGCCTACAACATCTCCGTCTTCTCAGTGAAGCGAAACAGTAACAACAAGAATGACATCAGCAGGCCTGCCGTGCTGCTGGCCCGCACGCGACCCCGCCCTGTGGAAGGCTTCGAGGTCACCAATGTGACGGCTAGCACCATCTCAGTGCAGTGGGCCCTGCACAGGATCCGCCATGCCACCGTCAGTGGGGTCCGTGTGTCCATCCGCCACCCTGAGGCCCTCAGGGACCAGGCCACCGATGTGGACAGGAGTGTGGACAGGTTCACCTTTAGGGCCCTGCTGCCTGGGAAGAGGTACACCATCCAGCTGACCACCCTCAGTGGGCTCAGGGGAGAGGAGCACCCCACAGAGAGCCTGGCCACCGCGCCGACGCACGTGTGGACCCGGCCCCTGCCTCCAGCAAACCTGACCGCCGCCCGAGTCACTGCCACCTCTGCCCACGTGGTCTGGGATGCCCCGACTCCAGGCAGCTTGCTGGAGGCTTATGTCATCAATGTGACCACCAGCCAGAGCACCAAGAGCCGCTATGTCCCCAACGGGAAGCTGGCGTCCTACACGGTGCGCGACCTGCTGCCGGGACGGCGGTACCAGCCCTCTGTGATAGCAGTGCAGAGCACGGAGCTCGGGCCGCAGCAGCACCAGGGAGGACACCACCCTCGGGTGCTCAAGAACAGACCGCCCCCGGCGCGCCTGCCGGAGCTGCGCCTGCTCAATGACCACAGCGCCCCCGAGACCCCCACCCAGCCCCCCAGGTTCTCGGAGTTTGTGGACGGCAGAGGAAGAGTGAGCGCCAGGTTCGGTGGCTCACCCAGCAAAGCAGCCACCGTGAGATCACAACCCACAGCCTCGGCGCAGCTCGAGAACATGGAGGAAGCCCCCAAGCGGGTCAGCCCGGCCCTCCAGCTCCCTGAACACGGCAGCAAGGACATCGGAAACGTCCCTGGCAACTGTTCAGAAAACCCCTGTCAGAACGGAGGCACTTGTGTGCCGGGCGCAGACGCCCACAGCTGTGACTGCGGGCCAGGGTTCAAAGGCAGACGCTGCGAGCTCGCCTGTATAAAGGTGTCCCGCCCCTGCACAAGGCTGTTCTCCGAGACAAAGGCCTTTCCAGTCTGGGAGGGAGGCGTCTGTCACCACGTGTATAAAAGAGTCTACCGAGTTCACCAAGACATCTGCTTCAAAGAGAGCTGTGAAAGCACAAGCCTCAAGAAGACCCCAAACAGGAAACAAAGTAAGAGTCAGACACTGGAGAAATCTTAAGGATTTAAGACGTTCTTGTTACACTCCACCAACCTCACGAGTTTCTAACACCCAGGAAGATGAGGTCTAAAAACTGGATGAAAAAGCACACCCTGAGAAAAGGTCCTAGCTGGAGTCAGTCCCCTCTGTGACCTCTCTCCTCAGGCCTCTAGAGCACAGATGGCCAGGCCTGTGCACACACCAGCCCACCCTGAGAGACCCCTCTGGGACCAACCACCTGTGAGTCCTGTGATGCGTTTAAGCAGCCTGTGCCCTCACCCAAGCTGCAGTTCCTGAAGGTGTAGTCTGTGTCTCTGCGGATGAGATGACAGCTCGCCATTCCCCGGAATCAGTGAGGCTGTCAGTCAGCCACGCTTCTGCAGTATGCAGAAACCTGTTCTTAGACTCCAAAGCCAGAGAAAGAATTCTCCCTTCGAGGCCCAACAAATTGAGAAGGAACTGTGATGGACCACTTCCAAAACAGAGACGGGGGCAGGGGCTGAAGGGCAGAGACCAGGTGATGTCAGAAGGAAAGCCGGGTTGCAGACACAGCCGCCCCTGCTCTGGTCCTCCAGCGTGTTTATGACGCTCGTGCAGGTCGACGAGCCATCCTATGGACTAGTTAACACTAAGGTGGAGTTCAGACTTTTTTAGACAACGGCGCGACTGGCAGCCTTTCTCTATCAAGGGTCAGACGGTAAACGTTTTCAGCTTTGCAGACCAGAGGTCCCTGTGGCTACAGTAGCGCAGACACAGCCACAGGCATGTCATTGAATGGCTGCGGCTATGTTCCAATAAAAACTTATTTACAATAACAGGTGGTGGCCAAATTGGCCCATGGGCCTTATTTGGTGAACCCTGTTCTATGAGATCACCTAGGCTTCAGCCTTAAACAGTGGAAGCCATCCCCTGAATGACAAGTCACAAGGGTATCAAAGAAAGACCCCTGAATTTTCATGGAAAAAGCTATTCAGACCCCTGCTTGGAAAGCTAAGGCACACTGCCACGAAGCAGCAAGGACGCCTTACAAGTCTCAGTGCAACAGAGATGGACACCTGGGCTGGGCTGGACAATGTTTAAGGTTCCTTTTAGTCCATGACTCAAGTGATACTGTTTTAGGCTATCAGGTAGTAAACACGATCTTAGACATCCCCATCTTTGTAAGCAGAACAGTACGGCACTTCACCACATCTGCTTCCCACCATGCTTCTAAGCAGCTGTCTTCCCCCTGCTAATGTTACAACCAAAGCAGCCACCCCACCTCCTCTCGTGTTGAGCCTCACGACCGCTGACCCAGCTGGAAAGCCAGCGCCCTGCCGCGTCACCCTGACTCTGCTCAGAGCCAGCATTCCAGCCACAAAGAGGGCCTCCTTCCTTTCCTCTTTCATAAAAATGTTTTTTGAAGAGTTAGAGTATATTTTAGGCTTTTTATCTTTATTAAAATTTCATGTGCATGTGTANOV58f, SNP13382519 ofSEQ ID NO:11541268 aaMW at 136576.5 kDCG93387-05, Protein SequenceSNP Change: no changeMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTIFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCIALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGDHPVPDACLSAPCHNGGTCVDADQGYVCECPEGFMGLDCRERVPDDCECRNGGRCLGANTTLCQCPLGFFGLLCEFEITAMPCNMNTQCPDGGYCMEHGGSYLCVCHTDHNASHSLPSPCDSDPCFNGGSCDAHDDSYTCECPRGFHGKHCEKARPHLCSSGPCRNGGTCKEAGGEYHCSCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCVNGGTCEDRDTDFFCHCQAGYMGRRCQAEVDCGPPEEVKHATLRFNGTRLGAVALYACDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYEGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFHALLPGKRYTIQLTTLSGLRGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQQHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPSKAATVRSQPTASAQLENMEEAPKRVSPALQLPEHGSKDIGNVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLEKS


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 58B.

TABLE 58BComparison of the NOV58 protein sequences.NOV58aMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTI(SEQ ID NO:1144)NOV58bMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTI(SEQ ID NO:1146)NOV58cMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTI(SEQ ID NO:1148)NOV58d------------------------------------------------------------(SEQ ID NO:1150)NOV58eMLRRATEDVRHYFPELLDFNATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGKLSFTI(SEQ ID NO:1152)NOV58aFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVNOV58bFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVNOV58cFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTIVNOV58d------------------------------------------------------------NOV58eFNYESIVWTTGTHASSGGNATGLGGIAAQAGFNAGDGQRYFSIPGSRTADMAEVETTTNVNOV58aGVPGRWAFRIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGNOV58bGVPGRWAFRIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGNOV58cVVPGRWAFIIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGNOV58d------------------------------------------------------------NOV58eGVPGRWAFRIDDAQVRVGGCGHTTSVCLALRPCLNGGKCIDDCVTGNPSYTCSCLSGFTGNOV58aRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQNOV58bRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQNOV58cRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQNOV58d------------------------------------------------------------NOV58eRRCHLDVNECASQPCQNGGTCTHGINSFRCQCPAGFGGPTCETAQSPCDTKECQHGGQCQNOV58aVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGNOV58bVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGNOV58cVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGNOV58d------------------------------------------------------------NOV58eVENGSAVCVCQAGYTGAACEMDVDDCSPDPCLNGGSCVDLVGNYTCLCAEPFKGLRCETGNOV58aDNPVPDACLSAPCHNGGTCVDADQGYVCE-CPEGFMGLDCRERVPDDCECRNGGRCLGANNOV58bDHPVPDACLSAPCHNGGTCVDADQGYVCE-CPEGFMGLDCRERVPDDCECRNGGRCLGANNOV58cDHXQCQTPASRPLATMGAPVWMRTRATCASAPKASWAWTAGRESPMTVSAATEADAWAPTNOV58d-----------------------------------MGLDCRERVPDDCECRNGGRCLGANNOV58eDHPVPDACLSAPCHNGGTCVDADQGYVCE-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-------IDECRSQPCLHGGSCQDRVAGYLCLCSTGYENOV58dDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYENOV58eDRGYSLSAPSRIRVCQPHGVWSEPPQCLEIDECRSQPCLHGGSCQDRVAGYLCLCSTGYENOV58aGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCNOV58bGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCNOV58cGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCNOV58dGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCNOV58eGAHCELERDECRAHPCRNGGSCRNLPGAYVCRCPAGFVGVHCETEVDACDSSPCQHGGRCNOV58aESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKNOV58bESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKNOV58cESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKNOV58dESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKNOV58eESGGGAYLCVCPESFFGYHCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGEDCAKNOV58aELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSNOV58bELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSNOV58cELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSNOV58dELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSNOV58eELFPPTALKMERVEESGVSISWNPPNGPAARQMLDGYAVTYVSSDGSYRRTDFVDRTRSSNOV58aHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQNOV58bHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQNOV58cHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQNOV58dHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQNOV58eHQLQALAAGRAYNISVFSVKRNSNNKNDISRPAVLLARTRPRPVEGFEVTNVTASTISVQNOV58aWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRNOV58bWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRNOV58cWALHRIRHATVSGVRVSIRHPFALRDQATDVDRSVDRFTFPALLPGKRYTIQLTTLSGLRNOV58dWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRNOV58eWALHRIRHATVSGVRVSIRHPEALRDQATDVDRSVDRFTFRALLPGKRYTIQLTTLSGLRNOV58aGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQNOV58bGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQNOV58cGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQNOV58dGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQNOV58eGEEHPTESLATAPTHVWTRPLPPANLTAARVTATSAHVVWDAPTPGSLLEAYVINVTTSQNOV58aSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQQ------------------NOV58bSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQHSEPAHLYIITSPRDGADRNOV58cSTKSRYVPNGKLASYTVRDLLPGRRYQLSVIAVQSTELGPQHSEPAHLYIITSPRDGADRNOV58dSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQHSEPAHLYIITSPRDGADRNOV58eSTKSRYVPNGKLASYTVRDLLPGRRYQPSVIAVQSTELGPQHSEPAHLYIITSPRDGADRNOV58a--HQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPNOV58bRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPNOV58cRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSELVDGRGRVSARFGGSPNOV58dRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPNOV58eRWHQGGHHPRVLKNRPPPARLPELRLLNDHSAPETPTQPPRFSEFVDGRGRVSARFGGSPNOV58aSKAATVRS----------------------------------------------------NOV58bSKAATVRS----------------------------------------------------NOV58cSKAATVRSRPVPYMMSPPPPPAQSPASDPHPDCAQGAGLVGRRQHAPPWQEGQLGHPCSLNOV58dSKAATVRS----------------------------------------------------NOV58eSKAATVRS----------------------------------------------------NOV58a-----------------------------QPTASAQLENMEEAPKRVSPALQLPEHGSKDNOV58b-----------------------------QPTASAQLENMEEAPKRVSPALQLPEHGSKDNOV58cRYRSTQKGGPRTIPGAKQLRRVVTAWLLLEPTASAQLENMEEAPKRVSLALQLPEHGSKDNOV58d-----------------------------QPTASAQLENMEEAPKRVSPALQLPEHGSKDNOV5Be-----------------------------QPTASAQLENMEEAPKRVSPALQLPEHGSKDNOV58aIG----------------------------------------------------------NOV58bIG----------------------------------------------------------NOV58cIGSYAGPELSPSPGLCLVRIEASTSLQGTKHLLSTCSTQAKEQGGAFTLPCLCGPVQGDHNOV58dIG----------------------------------------------------------NOV58eVG----------------------------------------------------------NOV58a---------------NVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSNOV58b---------------NVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSNOV58cGVGEGKPVFTDHSPADVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELG-IKESNOV58d---------------NVPGNCSENPCQNGGTCVPGADAHSCDCGPGFKGRRCELACIKVSNOV58e---------------NVPGNCSENPCQNGGTCVPGADAHSCGCGPGFKGRRCELACIKVSNOV58aRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNPKQSKSQTLNOV58bRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLNOV5BcTEFTKTSASKRAVKAQAS-----------RRPQTGASGEQAHAVSCM-------------NOV58dRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNPKQSKSQTLNOV58eRPCTRLFSETKAFPVWEGGVCHHVYKRVYRVHQDICFKESCESTSLKKTPNRKQSKSQTLNOV58aEKSNOV58bEKSNOV58c---NOV58dEKSNOV58eEKS


Further analysis of the NOV58a protein yielded the following properties shown in Table 58C.

TABLE 58CProtein Sequence Properties NOV58aSignalP analysis:Cleavage site between residues 41 and 42PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 10; pos. chg 3; neg. chg 2H-region:length 4; peak value −6.27PSG score:−10.68GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −3.98possible cleavage site: between 40 and 41>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 2.12 (at 365)ALOM score: 2.12 (number of TMSs: 0)MITDISC:discrimination of mitochondrial targeting seqR content: 2Hyd Moment(75):18.26Hyd Moment(95):17.56G content: 0D/E content: 2S/T content: 1Score: −1.90Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 14 RRA|TENUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PGFKGRR (3) at 1190bipartite: nonecontent of basic residues: 9.5%NLS Score: −0.22KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: LRRAKKXX-like motif in the C-terminus: TLEKSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):52.2%: nuclear34.8%: mitochondrial 8.7%: cytoplasmic 4.3%: cytoskeletal>> prediction for CG93387-05 is nuc (k = 23)


A search of the NOV58a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 58D.

TABLE 58DGeneseq Results for NOV58aNOV58aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #,Date]ResiduesRegionValueAAB82249Human insulin-responsive sequence261 . . . 1268 1004/1028(97%)0.0DNA binding protein-1 - Homo1 . . . 10281004/1028(97%)sapiens, 1028 aa. [WO200132873-A1,10-MAY-2001]AAB82247Rat insulin-responsive sequence DNA271 . . . 1253 797/1003(79%)0.0binding protein-1 - Rattus sp, 1008 aa.1 . . . 1002876/1003(86%)[WO200132873-A1, 10-MAY-2001]AAB42900Human ORFX ORF2664 polypeptide1 . . . 627 592/629(94%)0.0sequence SEQ ID NO: 5328 - Homo61 . . . 689 593/629(94%)sapiens, 694 aa. [WO200058473-A2,05-OCT-2000]AAB82251Rat insulin-responsive sequence DNA780 . . . 1253 368/494(74%)0.0binding protein-1 (truncated) - Rattus1 . . . 493 414/494(83%)sp, 499 aa. [WO200132873-A1, 10-MAY-2001]AAB82250Human insulin-responsive sequence813 . . . 1161 344/369(93%)0.0DNA binding protein-1 (variant) - Homo1 . . . 369 345/369(93%)sapiens, 387 aa. [WO200132873-A1,10-MAY-2001]


In a BLAST search of public sequence databases, the NOV58a protein was found to have homology to the proteins shown in the BLASTP data in Table 58E.

TABLE 58EPublic BLASTP Results for NOV58aNOV58aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ8TER0FLJ00133 protein - Homo sapiens 7 . . . 12681258/1282(98%)0.0(Human), 1282 aa (fragment). 1 . . . 12821258/1282(98%)AAO41836Secreted protein SST3 - Mus 1 . . . 939800/939(85%)0.0musculus (Mouse), 1065 aa.126 . . . 1064862/939(91%)Q8N369Hypothetical protein - Homo sapiens666 . . . 1252549/607(90%)0.0(Human), 600 aa.26 . . . 599551/607(90%)Q8TEP7FLJ00146 protein - Homo sapiens706 . . . 1268498/583(85%)0.0(Human), 522 aa (fragment). 1 . . . 522499/583(85%)P10079Fibropellin I precursor (Epidermal140 . . . 777 261/679(38%)e−145growth factor-related protein 1)249 . . . 895 339/679(49%)(UEGF-1) - Strongylocentrotuspurpuratus (Purple sea urchin), 1064aa.


PFam analysis predicts that the NOV58a protein contains the domains shown in the Table 58F.

TABLE 58FDomain Analysis of NOV58aIdentities/Similarities forPfamNOV58athe MatchedExpectDomainMatch RegionRegionValueEGF147 . . . 18316/47 (34%)3.4e−0528/47 (60%)EGF190 . . . 22115/47 (32%)6.9e−0828/47 (60%)EGF228 . . . 25913/47 (28%)1.4e−0521/47 (45%)EGF266 . . . 29717/47 (36%)1.5e−0926/47 (55%)EGF308 . . . 33918/47 (38%)2.9e−0925/47 (53%)EGF420 . . . 45117/47 (36%)4.3e−0725/47 (53%)EGF459 . . . 49016/47 (34%)1.5e−0526/47 (55%)EGF498 . . . 52918/47 (38%)4.9e−0929/47 (62%)EGF536 . . . 56715/47 (32%)4.7e−0622/47 (47%)sushi573 . . . 62616/64 (25%)3.8e−0536/64 (56%)EGF632 . . . 66314/47 (30%)7.8e−0721/47 (45%)EGF670 . . . 70117/47 (36%)3.3e−0723/47 (49%)EGF708 . . . 73913/47 (28%)1.4e−0525/47 (53%)EGF746 . . . 77713/47 (28%)3.5e−0526/47 (55%)fn3781 . . . 86224/88 (27%)3.9e−0860/88 (68%)fn3880 . . . 96318/87 (21%)  2e−0962/87 (71%)fn3 979 . . . 106127/86 (31%)9.3e−0858/86 (67%)EGF1166 . . . 119717/47 (36%)4.2e−0828/47 (60%)


Example 59

The NOV59 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 59A.

TABLE 59ANOV59 Sequence AnalysisNOV59a, CG93871-01SEQ ID NO: 11552018 bpDNA SequenceORF Start: ATG at 101ORF Stop: TAA at 1937CTCCCCACGGCGCCAGGAGGAGGGGCGAGGGCCGGCAGCCCCCTCTCCCGCGCGCGGCGCAGGAGCCCAGCCCAGCCCGGGGGACCCGCCGCCGCCGGTCATGTGGGCCGGACTGCTCCTTCGGGCCGCCTGTGTCGCGCTCCTGCTGCCGGGGGCACCAGCCCGAGGCTACACCGGGAGGAAGCCGCCCGGGCACTTCGCGGCCGAGAGGCGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCTGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCGTCTCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGTTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGGACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCCATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGATGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGTTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCGTGCATGTTTCGTGAGTGTCCTTTTGGCCCGTGTGAGACCCCCCATAAAGACAGATGCTATTTCCACGGCCGGTGGTACGCAGACGGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCTGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGTTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTGTAGATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCCCAGACTGTTCAGCAGGTAATCCCCTGCCTCTGCCCCAAGCCCCCAGGGCAGGGCATCTCAGGCATCGGGCTCCTTAAGCCCTATACAGCCTTCATCTCNOV59a, CG93871-01SEQ ID NO: 1156612 aaMW at 65156.4kDProtein SequenceMWAGLLLRAACVALLLPGAPARGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSMGGGHCTLRLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCMFRECPFGPCETPHKDRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPELACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCRWLGELQEDRLCGLLPSPDPDPWTVLPRLFSRNOV59b, CG93871-05SEQ ID NO: 11572819 bpDNA SequenceORF Start: ATG at 5ORF Stop: TAA at 2807GGTCATGTGGGCCGGACTGCTCCTTCGGGCCGCCTGTGTCGCGCTCCTGCTGCCGGGGGCACCAGCCCGAGGCTACACCGGGAGGAAGCCGCCCGGGCACTTCGCGGCCGAGAGACGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCTGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCCCCTCTGCTCCCTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGTTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGGACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCCATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGACGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGCTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCCTGCATCTCTCCTGAGTGTCCTTCTGGCCCCTGTCAGACCCCCCCACAGACGGATTGCTGTACTTGTGTTCCAGTGAGATGCTATTTCCACGGCCGGTGGTACGCAGACGGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCTGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGCTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTGTGAGTTATGCATCTGCCAGGCAGATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCCCAGACTGTTCAGCAGGCTGCACCTACACAGGCAGAATCTTCTATAACAACGAGACCTTCCCGTCTGTGCTGGACCCATGTCTGAGCTGCATCTGCCTGCTGGGCTCAGTGGCCTGTTCCCCCGTGGACTGCCCCATCACCTGTACCTACCCTTTCCACCCTGACGGGGAGTGCTGCCCCGTGTGCCGAGACTGCAACTACGAGGGAAGGAAGGTGGCGAATGGCCAGGTGTTCACCTTGGATGATGAACCCTGCACCCGGTGCACGTGCCAGCTGGGAGAGGTGAGCTGTGAGAAGGTTCCCTGCCAGCGGGCCTGTGCCGACCCTGCCCTGCTTCCTGGGGACTGCTGCTCTTCCTGTCCAGATTCCCTGTCTCCTCTGGAAGAAAAGCAGGGGCTCTCCCCTCACGGAAATGTGGCATTCAGCAAAGCTGGTCGGAGCCTGCATGGAGACACTGAGGCCCCTGTCAACTGTAGCTCCTGTCCTGGGCCCCCGACAGCATCACCCTCGAGGCCGGTGCTTCATCTCCTCCAGCTCCTTTTAAGAACGAACTTGATGAAAACACAGACTTTACCTACAAGCCCGGCAGGAGCTCATGGTCCACACTCACTCGCTTTGGGGCTGACAGCCACTTTCCCAGGGGAGCCTGGGGCCTCCCCTCGACTCTCACCAGGGCCTTCGACCCCTCCAGGAGCCCCCACTCTACCTCTAGCTTCCCCAGGGGCTCCTCAGCCACCTCCTGTGACTCCAGAGCGCTCGTTCTCAGCCTCTGGGGCCCAGATAGTGTCCAGGTGGCCTCCTCTGCCTGGCACCCTCCTGACGGAAGCTTCAGCACTTTCCATGATGGACCCCAGCCCCTCGAAGACCCCCATCACCCTCCTCGGGCCTCGCGTGCTTTCTCCCACCACCTCTAGACTCTCCACAGCCCTTGCAGCCACCACCCACCCTGGCCCCCAGCAGCCCCCAGTGGGGGCTTCTCGGGGGGAAGAGTCCACCATGTAAGGAGGTCACTNOV59b, CG93871-05SEQ ID NO: 1158934 aaMW at 97715.0kDProtein SequenceMWAGLLLRAACVALLLPGAPARGYTGRKPPGHFAAERRRLGPRVCLSGFGSGCCPGWAPSMGGGHCTLPLCSLGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRIAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPECPSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPELACPRBEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCELCICQADGSVSCKRTDCVDSCPHPIRIPGQCCPDCSAGCTYTGRIFYNNETFPSVLDPCLSCICLLGSVACSPVDCPITCTYPFHPDGECCPVCRDCNYEGRKVANGQVFTLDDEPCTRCTCQLGEVSCEKVPCQRACADPALLPGDCCSSCPDSLSPLEEKQGLSPHGNVAFSKAGRSLHGDTEAPVNCSSCPGPPTASPSRPVLHLLQLLLRTNLMKTQTLPTSPAGAHGPHSLALGLTATFPGEPGASPRLSPGPSTPPGAPTLPLASPGAPQPPPVTPERSFSASGAQIVSRWPPLPGTLLTEASALSMMDPSPSKTPITLLGPRVLSPTTSRLSTALAATTHPGPQQPPVGASRGEESTMNOV59c, 198488432SEQ ID NO: 11591814 bpDNA SequenceORF Start: at 1ORF Stop: at 1813GGTACCCGAGGCTACACCGGGAGGAAGCCGCCCGGGCACTTCGCGGCCGAGAGACGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCTGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCCCCCCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAAGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGTTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGGACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCCATGTATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGTTGGTGCGAGGACGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGCTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCCTGCATCTCTCCTGAGTGTCCTTCTGGCCCCTGTCAGACCCCCCCACAGACGGATTGCTGTACTTGTGTTCCAGTGAGATGCTATTTCCACGGCCGGTGGTACGCAGACGGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCTGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGCTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTGTGAGTTATGCATCTGCCAGGCAGATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCTCGAGNOV59c, 198488432SEQ ID NO: 11601604 aaMW at 63737.5kDProtein SequenceGTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSMGGGHCTLPPCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTESGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMYESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPECPSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPELACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCELCICQADGSVSCKRTDCVDSCPHPIRIPGQCCSNOV59d, 198488424SEQ ID NO: 11611790 bpDNA SequenceORF Start: at 1ORF Stop: at 1789GGTACCCGAGGCTACACCGGGAGGAAGCCGCCCGGGCACTTCGCGGCCGAGAGACGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCCGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCCCCTCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGCTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGAACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCGATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGACGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGCTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCCTGCATCTCTCCTGAGGGTCCTTCTGGCCCCTGTCAGACCCCCCCACAGACGGATTGCTGTACTTGTGTTCCAGTGAGATGCTATTTCCACGGCCGGTGGTACGCAGACGGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCTGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGCTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCTCGAGNOV59d, 198488424SEQ ID NO: 1162596 aaMW at 62805.5kDProtein SequenceGTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGHAPSMGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVPAEGDVFSPPNENCTVCVCLAGNVSCISPEGPSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPELACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPYGSVSCKRTDCVDSCPHPIRIPGQCCSNOV59e, 198488428SEQ ID NO: 11631661 bpDNA SequenceORF Start: at 1ORF Stop: at 1660GGTACCCGAGGCTACACCGGGAGGAAGCCGCCCGGGCACTTCGCGGCCGAGAGACGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCTGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCCCCTCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGGGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGTTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGCTTCCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGGACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCCATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGACGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGCTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCCTGCATCTCTCCTGAGTGTCCTTCTGGCCCCTGTCAGACCCCCCCACAGACGGATTGCTGTACTTGTGTTCCAGTGAGATGCTATTTCCACGGCCGGTGGTACGCAGACAGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCTGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGCTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCTCGAGNOV59e, 198488428SEQ ID NO: 1164553 aaMW at 57922.0kDProtein SequenceGTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSMGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVASPKAVLAPSAILQPRQHPSKNLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPECPSGPCQTPPQTDCCTCVPVRCYFHGRWYADRAVFSGGGDECTTCVCQNGEVECSFMPCPELACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPYGSVSCKRTDCVDSCPHPIRIPGQCCSNOV59f, 198488440SEQ ID NO: 11651814 bpDNA SequenceORF Start: at 1ORF Stop: at 1813GGTACCCGAGGCTACACCGGGAAGAAGCCGCCCGGGCACTTCGCGGCCGAGAGACGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCTGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCCCCTCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGCTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGGACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCGATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGACGGGAAGGTGACCTGTGAAAAGGTGCGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGCTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCCTGCATCTCTCCTGAGTGTCCTTCTGGCCCCTGTCAGACCCCCCCACAGACGGATTGCTGTACTTGTGTTCCAGTGAGATGCTATTTCCACGGCCGGTGGTACGCAGACGGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCCGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGCTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTGTGAGTTATGCATCTGCCAGGCAGATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCTCGAGNOV59f, 198488440SEQ ID NO: 1166604 aaMW at 63653.5kDProteing SequenceGTRGYTGKKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSMGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPECPSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPELACPREEWRPGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCELCICQADGSVSCKRTDCVDSCPHPIRIPGQCCSNOV59g, CG93871-02SEQ ID NO: 1167585 bpDNA SequenceORF Start: at 7ORF Stop: at 580GGTACCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCTAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGGGATGTAAAAACAGCATTGGCAGCTACAAGTGTTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTCGAGACAGGCTCCGAGCTGACCGCGTGTCCTGTCTCGAGNOV59g, CG93871-02SEQ ID NO: 1168191 aaMW at 20429.7kDProtein SequenceCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQGCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPRDRLRADRVSCNOV59h, CG93871-03SEQ ID NO: 11692063 bpDNA SequenceORF Start: ATG at 20ORF Stop: TAG at 1229AGTGTTACAGTTCTTCAAGATGGTGTGTCCGGAGTTTGTTCCTTCAGATGTTCAGATGTGTCCAGAATTTCTTCCTTCTGGTGGGTTCGTGCTCTCACTGGCTTCAATGGGTGGTGGGCACTGCACCCTGCCCCTCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGTTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGGACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGAGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCCATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGTGGGTACTAGGGGTCCCCGGAGCTTCCTTGGTCTGGGGCCTTGTGGTGGCTCTAACTCCTGCTTGTGCTTCTAGGACGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGTGAGAGCTGGTGCCAGGGAAGGACATAGTCCTGCCTCTTCCTTTCTCTGCACCCTCCTGCAGCCAAAAGGCTGTTCTTTAACATTAGCATACATTAGAATCTCCTGGAGGGTTTACTAAGCCACACTTCCCTTGGCGCTGCTCCCAGAGCTTCTGATGTAGTAGGTCTGGGATAGGGCCAGAACATGTGCGTTTTGAACAGGTTCCCAGGTGATACTGACGTGGCTGGTCTAGGGACCATACTTTGAGAACCACTCATTTATGCTGTAATATGCCTCAAGGGGGACTTTTCATCTTCTGGGCATCTGTTTTCAGCTTCATTCGCTCATTTTCATTTATTCAGTCATTCATTGAGCCTCTTTAAATCCACTGTGCAGTCCCCTTCTCTTTCCGACTCACCTTCTCACATGGGGAGGAGTAGTTGCTTTACAATTCCCCCCCTTTTTAATCTCCCCCCCCCCATATATCGATACTTCTTTATCTTCTTCCCATTCCTGGAAGAATAGGGACCCATATTCTTCACCAAATCCTCAACTAGAGTTTCTTCTGAACTACCTCTCCAGCCCCTCAAGGGTATTCTGATGACAAATACAAATTCTTCTCGTTGGCACGCTGTCTCTATCGCAGAACCCCACCACTTTGGGAGTCCAATTTTGTCTTATCACTTGAGCGGACCATTCAGGGACTGTTTGGGATACTCGGTGATAAACGTCGPGCATTCGTCTTTCTATCTCCTCANOV59h, CG93871-03SEQ ID NO: 1170403 aaMW at 42493.0kDProtein SequenceMVCPEFVPSDVQMCPEFLPSGGFVLSLASMGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMRTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEVGTRGPRSFLGLGPCGGSNSCLCFNOV59i, CG93871-04SEQ ID NO: 11711790 bpDNA SequenceORF Start: at 1ORF Stop: at 1789GGTACCCGAGGCTACACCGGGAGGAAGCCGCCCGGGCACTTCGCGGCCGAGAGACGCCGACTGGGCCCCCACGTCTGCCTCTCTGGGTTTGGGAGTGGCTGCTGCCCTGGCCGGGCGCCCTCTATGGGTGGTGGGCACTGCACCCTGCCCCTCTGCTCCTTCGGCTGTGGGAGTGGCATCTGCATCGCTCCCAATGTCTGCTCCTGCCAGGATGGAGAGCAAGGGGCCACCTGCCCAGAAACCCATGGACCATGTGGGGAGTACGGCTGTGACCTTACCTGCAACCATGGAGGCTGTCAGGAGGTGGCCCGAGTGTGCCCCGTGGGCTTCTCGATGACGGAGACAGCTGTTGGCATCAGGTGTACAGACATTGACGAATGTGTAACCTCCTCCTGCGAGGGCCACTGTGTGAACACAGAAGGTGGGTTTGTGTGCGAGTGTGGGCCGGGCATGCAGCTGTCTGCCGACCGCCACAGCTGCCAAGACACTGACGAATGCCTAGGGACTCCCTGTCAGCAGAGATGTAAAAACAGCATTGGCAGCTACAAGTGCTCCTGTCGAACTGGCTTCCACCTTCATGGCAACCGGCACTCCTGTGTAGATGTAAACGAGTGTCGGAGGCCATTGGAGAGGCGAGTCTGTCACCATTCCTGCCACAACACCGTGGGCAGCTTCCTATGCACATGCCGACCTGGCTTCAGGCTCCGAGCTGACCGCGTGTCCTGTGAAGCTTTCCCGAAAGCCGTGCTGGCCCCATCTGCCATCCTGCAACCCCGGCAACACCCGTCCAAGATGCTTCTGTTGCTTCCTGAGGCCGGCCGGCCTGCCCTGTCCCCAGGACATAGCCCTCCTTCTGGGGCTCCAGGGCCCCCAGCCGGAGTCAGAACCACCCGCCTGCCATCTCCCACCCCACGACTACCCACATCCTCCCCTTCTGCCCCTGTGTGGCTGCTGTCCACCCTGCTGGCCACCCCAGTGCCTACTGCCTCCCTGCTGGGGAACCTCAGACCCCCCTCACTCCTTCAGGGGGAGGTGATGGGGACCCCTTCCTCACCCAGGGGCCCTGAGTCCCCCCGACTGGCAGCAGGGCCCTCTCCCTGCTGGCACCTGGGAGCGATGCATGAATCAAGGAGTCGCTGGACAGAGCCTGGGTGTTCCCAGTGCTGGTGCGAGGACGGGAAGGTGACCTGTGAAAAGGTGAGGTGTGAAGCTGCTTGTTCCCACCCAATTCCCTCCAGAGATGGTGGGTGCTGCCCATCGTGCACAGGCTGTTTTCACAGTGGTGTCGTCCGAGCTGAAGGGGATGTGTTTTCACCTCCCAATGAGAACTGCACCGTCTGTGTCTGTCTGGCTGGAAACGTGTCCTGCATCTCTCCTGAGGGTCCTTCTGGCCCCTGTCAGACCCCCCCACAGACGGATTGCTGTACTTGTGTTCCAGTGAGATGCTATTTCCACGGCCGGTGGTACGCAGACGGGGCTGTGTTCAGTGGGGGTGGTGACGAGTGTACCACCTGTGTTTGCCAGAATGGGGAGGTGGAGTGCTCCTTCATGCCCTGCCCTGAGCTGGCCTGCCCCCGAGAAGAGTGGCGGCTGGGCCCTGGGCAGTGTTGCTTCACCTGCCAGGAGCCCACACCCTCGACAGGCTGCTCTCTTGACGACAACGGGGTTGAGTTTCCGATTGGACAGATCTGGTCGCCTGGTGACCCCTATGGCTCGGTGAGCTGCAAGAGGACAGACTGTGTGGACTCCTGCCCTCACCCGATCCGGATCCCTGGACAGTGCTGCTCGAGNOV59i, CG93871-04SEQ ID NO: 1172596 aaMW at 62805.5kDProtein SequenceGTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGRAPSMGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQEVARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQDTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTVGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHSPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLQGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRCEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPEGPSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPELACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPYGSVSCKRTDCVDSCPHPIRIPGQCCS


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 59B.

TABLE 59BComparison of the NOV59 protein sequences.NOV59a MWAGLLLRAACVALLLPGAPARGYTGRKPPGHFAAERRRLGPNVCLSGFGSGCCPGWAPSNOV59b MWAGLLLRAACVALLLPGAPARGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSNOV59c -------------------GTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSNOV59d -------------------GTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGRAPSNOV59e -------------------GTRGYTGRKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSNOV59f -------------------GTRGYTGKKPPGHFAAERRRLGPHVCLSGFGSGCCPGWAPSNOV59g ------------------------------------------------------------NOV59h -------------MVCP--------------EFVPS----DVQMCPEFLPSGGFVLSLASNOV59i -------------------GTRGYTGRKPPGHFAAERPRLGPHVCLSGFGSGCCPGRAPSNOV59a MGGGHCTLRLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59b MGGGHCTLPLCSLGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59c MGGGHCTLPPCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59d MGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59e MGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59f MGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59g ----------CSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59h MGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59i MGGGHCTLPLCSFGCGSGICIAPNVCSCQDGEQGATCPETHGPCGEYGCDLTCNHGGCQENOV59a VARVCPVGFSMTETAVGIRC-DIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59b VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTE---------------------NOV59c VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTESGFVCECGPGMQLSADRHSCQNOV59d VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59e VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59f VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59g VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59h VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59i VARVCPVGFSMTETAVGIRCTDIDECVTSSCEGHCVNTEGGFVCECGPGMQLSADRHSCQNOV59a DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59b DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59c DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59d DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59e DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVAS-------------------NOV59f DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59g DTDECLGTPCQQGCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59h DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59i DTDECLGTPCQQRCKNSIGSYKCSCRTGFHLHGNRHSCVDVNECRRPLERRVCHHSCHNTNOV59a VGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59b VGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59c VGSFLCTCRPGFRLEADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59d VGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59e ------------------------PKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59f VGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59g VGSFLCTCRPRDRLRADRVSC---------------------------------------NOV59h VGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59i VGSFLCTCRPGFRLRADRVSCEAFPKAVLAPSAILQPRQHPSKMLLLLPEAGRPALSPGHNOV59a SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59b SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59c SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59d SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59e SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59f SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59g ------------------------------------------------------------NOV59h SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59i SPPSGAPGPPAGVRTTRLPSPTPRLPTSSPSAPVWLLSTLLATPVPTASLLGNLRPPSLLNOV59a QGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59b QGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59c QGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMYESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59d QGEVMGTPSSPRGPESPRIAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59e QGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59f QGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59g ------------------------------------------------------------NOV59h QGEVMRTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEVG--------NOV59i QGEVMGTPSSPRGPESPRLAAGPSPCWHLGAMHESRSRWTEPGCSQCWCEDGKVTCEKVRNOV59a CEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCMFRECNOV59b CEAACSHPIPSRDGGCCPSCTGCFHSGVVPAEGDVFSPPNENCTVCVCLAGNVSCISPECNOV59c CEAACSHPIPSRDGGCCPSCTGCFHSGVVPAEGDVFSPPNENCTVCVCLAGNVSCISPECNOV59d CEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPEGNOV59e CEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPECNOV59f CEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPECNOV59g ------------------------------------------------------------NOV59h ----------------------------TRGPRSFLG-----------------------NOV59i CEAACSHPIPSRDGGCCPSCTGCFHSGVVRAEGDVFSPPNENCTVCVCLAGNVSCISPEGNOV59a PFGPCETPHK--D------RCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59b PSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59c PSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59d PSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59e PSGPCQTPPQTDCCTCVPVRCYFHGRWYADRAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59f PSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59g ------------------------------------------------------------NOV59h -LGPCGGSNS--C------LCF--------------------------------------NOV59i PSGPCQTPPQTDCCTCVPVRCYFHGRWYADGAVFSGGGDECTTCVCQNGEVECSFMPCPENOV59a LACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCR-------WLGNOV59b LACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCELCICQADGSVNOV59c LACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQTWSPGDPCELCICQADGSVNOV59d LACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQTWSPGDP--------YGSVNOV59e LACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGD--------PYGSVNOV59f LACPREEWRPGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDPCELCICQADGSVNOV59g ------------------------------------------------------------NOV59h ------------------------------------------------------------NOV59i LACPREEWRLGPGQCCFTCQEPTPSTGCSLDDNGVEFPIGQIWSPGDP--------YGSVNOV59a ELQEDRLCGLLPSPDPDPWTVLPRLFSR--------------------------------NOV59b SCKRTDCVDSCPHPIRIPGQCCPDCSAGCTYTGRIFYNNETFPSVLDPCLSCICLLGSVANOV59c SCKRTDCVDSCPHPIRIPGQCCS-------------------------------------NOV59d SCKRTDCVDSCPHPIRIPGQCCS-------------------------------------NOV59e SCKRTDCVDSCPHPIRIPGQCCS-------------------------------------NOV59f SCKRTDCVDSCPHPIRIPGQCCS-------------------------------------NOV59g ------------------------------------------------------------NOV59h ------------------------------------------------------------NOV59i SCKRTDCVDSCPHPIRIPGQCCS-------------------------------------NOV59a ------------------------------------------------------------NOV59b CSPVDCPITCTYPFHPDGECCPVCRDCNYEGRKVANGQVFTLDDEPCTRCTCQLGEVSCENOV59c ------------------------------------------------------------NOV59d ------------------------------------------------------------NOV59e ------------------------------------------------------------NOV59f ------------------------------------------------------------NOV59g ------------------------------------------------------------NOV59h ------------------------------------------------------------NOV59i ------------------------------------------------------------NOV59a ------------------------------------------------------------NOV59b KVPCQRACADPALLPGDCCSSCPDSLSPLEEKQGLSPHGNVAFSKAGRSLHGDTEAPVNCNOV59c ------------------------------------------------------------NOV59d ------------------------------------------------------------NOV59e ------------------------------------------------------------NOV59f ------------------------------------------------------------NOV59g ------------------------------------------------------------NOV59h ------------------------------------------------------------NOV59i ------------------------------------------------------------NOV59a ------------------------------------------------------------NOV59b SSCPGPPTASPSRPVLHLLQLLLRTNLMKTQTLPTSPAGAHGPHSLALGLTATFPGEPGANOV59c ------------------------------------------------------------NOV59d ------------------------------------------------------------NOV59e ------------------------------------------------------------NOV59f ------------------------------------------------------------NOV59g ------------------------------------------------------------NOV59h ------------------------------------------------------------NOV59i ------------------------------------------------------------NOV59a ------------------------------------------------------------N0V59b SPRLSPGPSTPPGAPTLPLASPGAPQPPPVTPERSFSASGAQIVSRWPPLPGTLLTEASANOV59c ------------------------------------------------------------NOV59d ------------------------------------------------------------NOV59e ------------------------------------------------------------NOV59f ------------------------------------------------------------NOV59g ------------------------------------------------------------NOV59h ------------------------------------------------------------NOV59i ------------------------------------------------------------NOV59a -------------------------------------------------------NOV59b LSMMDPSPSKTPITLLGPRVLSPTTSRLSTALAATTHPGPQQPPVGASRGEESTMNOV59c -------------------------------------------------------NOV59d -------------------------------------------------------NOV59e -------------------------------------------------------NOV59f -------------------------------------------------------NOV59g -------------------------------------------------------NOV59h -------------------------------------------------------NOV59i -------------------------------------------------------NOV59a (SEQ ID NO: 1156)NOV59b (SEQ ID NO: 1158)NOV59c (SEQ ID NO: 1160)NOV59d (SEQ ID NO: 1162)NOV59e (SEQ ID NO: 1164)NOV59f (SEQ ID NO: 1166)NOV59g (SEQ ID NO: 1168)NOV59h (SEQ ID NO: 1170)NOV59i (SEQ ID NO: 1172)


Further analysis of the NOV59a protein yielded the following properties shown in Table 59C.

TABLE 59CProtein Sequence Properties NOV59aSignalP analysis:Cleavage site between residues 22 and 23PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 8; pos. chg 1; neg. chg 0H-region:length 13; peak value 9.31PSG score:4.91GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 1.18possible cleavage site: between 21 and 22>>> Seems to have a cleavable signal peptide (1 to 21)ALOM:Klein et al's method for TM region allocationInit position for calculation: 22Tentative number of TMS(s) for the threshold 0.5: 0number of TMS(s) . . . fixedPERIPHERALLikelihood = 1.70 (at 70)ALOM score: 1.70 (number of TMSs: 0)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 10Charge difference: 0.5 C(2.5)-N(2.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptideMITDISC:discrimination of mitochondrial targeting seqR content:3Hyd Moment(75):9.30Hyd Moment(95):7.43G content:5D/E content:1S/T content:1Score: −3.74Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 79 LRL|CSNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 8.3%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/NucleardiscriminationPrediction: nuclearReliability: 89COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):33.3%: extracellular, including cell wall33.3%: mitochondrial33.3%: nuclear>> prediction for CG93871-01 is exc (k = 9)


A search of the NOV59a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 59D.

TABLE 59DGeneseq Results for NOV59aNOV59aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #,Date]ResiduesRegionValueABU12085Human NOV26a CG93871-01 protein1 . . . 612612/612(100%)0.0SEQ ID 90 - Homo sapiens, 612 aa.1 . . . 612612/612(100%)[WO200281625-A2, 17-OCT-2002]ABU11798Human MDDT polypeptide SEQ ID381 . . . 580 192/208(92%)e−126745 - Homo sapiens, 514 aa.1 . . . 208195/208(93%)[WO200279449-A2, 10-OCT-2002]AAB85364Novel Von Willebrand/thrombosporin-283 . . . 490 201/208(96%)e−124like polypeptide - Homo sapiens, 2351 . . . 208203/208(96%)aa. [WO200153485-A1, 26-JUL-2001]AAM99920Human polypeptide SEQ ID NO 36 -388 . . . 580 185/201(92%)e−119Homo sapiens, 272 aa.5 . . . 205188/201(93%)[WO200155173-A2, 02-AUG-2001]AAM99933Human polypeptide SEQ ID NO 49 -388 . . . 580 181/201(90%)e−117Homo sapiens, 212 aa.5 . . . 205185/201(91%)[WO200155173-A2, 02-AUG-2001]


In a BLAST search of public sequence databases, the NOV59a protein was found to have homology to the proteins shown in the BLASTP data in Table 59E.

TABLE 59EPublic BLASTP Results for NOV59aNOV59aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96DN2Hypothetical protein FLJ32009 -1 . . . 580570/589 (96%)0.0Homo sapiens (Human), 955 aa.1 . . . 589573/589 (96%)AAH434731300015B04Rik protein - Mus1 . . . 606507/615 (82%)0.0musculus (Mouse), 608 aa.1 . . . 607538/615 (87%)AAO47606URG11 - Homo sapiens (Human),283 . . . 580 290/306 (94%)0.0673 aa.1 . . . 306293/306 (94%)Q8IVT0Similar to hypothetical protein1 . . . 218216/219 (98%)    e−137FLJ32009 - Homo sapiens (Human),19 . . . 237 217/219 (98%)237 aa (fragment).Q8IXK1C1q receptor protein precursor -80 . . . 375 104/300 (34%)   6e−32Homo sapiens (Human), 652 aa.300 . . . 566 134/300 (44%)


PFam analysis predicts that the NOV59a protein contains the domains shown in the Table 59F.

TABLE 59FDomain Analysis of NOV59aIdentities/PfamNOV59aSimilarities forExpectDomainMatch Regionthe Matched RegionValueEGF145 . . . 17816/47 (34%)0.004723/47 (49%)EGF184 . . . 21712/47 (26%)0.01225/47 (53%)TIL165 . . . 22313/70 (19%)0.5340/70 (57%)EGF223 . . . 26012/48 (25%)0.04526/48 (54%)vwc385 . . . 43921/84 (25%)7.8e−0840/84 (48%)vwc442 . . . 49218/84 (21%)0.0001739/84 (46%)vwc493 . . . 55021/84 (25%)1.8e−0740/84 (48%)


Example 60

The NOV60 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 60A.

TABLE 60ANOV60 Sequence AnalysisNOV60a, CG94946-01SEQ ID NO: 11736224 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 6196CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGATGCTCAACTCCAGCCTCATGCGGATCACCCTGCGGAACCTGGAGGAGGTGGAGTTCTGTGTGGAAGATAAACCCGGGACCCACTTCACTCCAGTGCCTCCGACGCCTCCTGATGCGTGCCGGGGAATGCTGTGCGGCTTCGGCGCCGTGTGCGAGCCCAACGCGGAGGGGCCGGGCCGGGCGTCCTGCGTCTGCAAGAAGAGCCCGTGCCCCAGCGTGGTGGCGCCTGTGTGTGGGTCGGACGCCTCCACCTACAGCAACGAATGCGAGCTGCAGCGGGCGCAGTGCAGCCAGCAGCGCCGCATCCGCCTGCTCAGCCGCGGGCCGTGCGGCTCGCGGGACCCCTGCTCCAACGTGACCTGCAGCTTCGGCAGCACCTGTGCGCGCTCGGCCGACGGGCTGACGGCCTCGTGCCTGTGCCCCGCGACCTGCCGTGGCGCCCCCGAGGGGACCGTCTGCGGCAGCGACGGCGCCGACTACCCCGGCGAGTGCCAGCTCCTGCGCCGCGCCTGCGCCCGCCAGGAGAATGTCTTCAAGAAGTTCGACGGCCCTTGTGACCCCTGTCAGGGCGCCCTCCCTGACCCGAGCCGCAGCTGCCGTGTGAACCCGCGCACGCGGCGCCCTGAGATGCTCCTACGGCCCGAGAGCTGCCCTGCCCGGCAGGCGCCAGTGTGTGGGGACGACGGAGTCACCTACGAAAACGACTGTGTCATGGGCCGATCGGGGGCCGCCCGGGGTCTCCTCCTGCAGAAAGTGCGCTCCGGCCAGTGCCAGGGTCGAGACCAGTGCCCGGAGCCCTGCCGGTTCAATGCCGTGTGCCTGTCCCGCCGTGGCCGTCCCCGCTGCTCCTGCGACCGCGTCACCTGTGACGGGGCCTACAGGCCCGTGTGTGCCCAGGACGGGCGCACGTATGACAGTGATTGCTGGCGGCAGCAGGCTGAGTGCCGGCAGCAGCGTGCCATCCCCAGCAAGCACCAGGGCCCGTGTGACCAGGCCCCGTCCCCATGCCTCGGGGTGCAGTGTGCATTTGGGGCGACGTGTGCTGTGAAGAACGGGCAGGCAGCGTGTGAATGCCTGCAGGCGTGCTCGAGCCTCTACGATCCTGTGTGCGGCAGCGACGGCGTCACATACGGCAGCGCGTGCGAGCTGGAGGCCACGGCCTGTACCCTCGGGCGGGAGATCCAGGTGGCGCGCAAAGGACCCTGTGACCGCTGCGGGCAGTGCCGCTTTGGAGCCCTGTGCGAGGCCGAGACCGGGCGCTGCGTGTGCCCCTCTGAATGCGTGGCTTTGGCCCAGCCCGTGTGTGGCTCCGACGGGCACACGTACCCCAGCGAGTGCATGCTGCACGTGCACGCCTGCACACACCAGATCAGCCTGCACGTGGCCTCAGCTGGACCCTGTGAGACCTGTGGAGATGCCGTGTGTGCTTTTGGGGCTGTGTGCTCCGCAGGGCAGTGTGTGTGTCCCCGGTGTGAGCACCCCCCGCCCGGCCCCGTGTGTGGCAGCGACGGTGTCACCTACGGCAGTGCCTGCGAGCTACGGGAAGCCGCCTGCCTCCAGCAGACACAGATCGAGGAGGCCCGGGCAGGGCCGTGCGAGCAGGCCGAGTGCGGTTCCGGAGGCTCTGGCTCTGGGGAGGACGGTGACTGTGAGCAGGAGCTGTGCCGGCAGCGCGGTGGCATCTGGGACGAGGACTCGGAGGACGGGCCGTGTGTCTGTGACTTCAGCTGCCAGAGTGTCCCAGGCAGCCCGGTGTGCGGCTCAGATGGGGTCACCTACAGCACCGAGTGTGAGCTGAAGAAGGCCAGGTGTGAGTCACAGCGAGGGCTCTACGTAGCGGCCCAGGGAGCCTGCCGAGGCCCCGCCTTCGCCCCGCTGCCGCCTGTGGCCCCCTTACACTGTGCCCAGACGCCCTACGGCTGCTGCCAGGACAATATCACCGCAGCCCGGGGCGTGGGCCTGGCTGGCTGCCCCAGTGCCTGCCAGTGCAACCCCCATGGCTCTTACGGCGGCACCTGTGACCCAGCCACAGGCCAGTGCTCCTGCCGCCCAGGTGTGGGGGGCCTCAGGTGTGACCGCTGTGAGCCTGGCTTCTGGAACTTTCGAGGCATCGTCACCGATGGCCGGAGTGGCTGTACACCCTGCAGCTGTGATCCCCAAGGCGCCGTGCGGGATGACTGTGAGCAGATGACGGGGCTGTGCTCGTGTAAGCCCGGGGTGGCTGGACCCAAGTGTGGGCAGTGTCCAGACGGCCGTGCCCTGGGCCCCGCGGGCTGTGAAGCTGACGCTTCTGCGCCTGCGACCTGTGCGGAGATGCGCTGTGAGTTCGGTGCGCGGTGCGTGGAGGAGTCTGGCTCAGCCCACTGTGTCTGCCCGATGCTCACCTGTCCAGAGGCCAACGCTACCAAGGTCTGTGGGTCAGATGGAGTCACATACGGCAACGAGTGTCAGCTGAAGACCATCGCCTGCCGCCAGGGCCTGCAAATCTCTATCCAGAGCCTGGGCCCGTGCCAGGAGGCTGTTGCTCCCAGCACTCACCCGACATCTGCCTCCGTGACTGTGACCACCCCAGGGCTCCTCCTGAGCCAGGCACTGCCGGCCCCCCCCGGCGCCCTCCCCCTGGCTCCCAGCAGTACCGCACACAGCCAGACCACCCCTCCGCCCTCATCGCGACCTCGGACCACTGCCAGCGTCCCCAGGACCACCGTGTGGCCCGTGCTGACGGTGCCCCCCACGGCACCCTCCCCTGCACCCAGCCTGGTGGCGTCCGCCTTTGGTGAATCTGGCAGCACTGATGGAAGCAGCGATGAGGAACTGAGCGGGGACCAGGAGGCCAGTGGGGGTGGCTCTGGGGGGCTCGAGCCCTTGGAGGGCAGCAGCGTGGCCACCCCTGGGCCACCTGTCGAGAGGGCTTCCTGCTACAACTCCGCGTTGGGCTGCTGCTCTGATGGGAAGACGCCCTCGCTGGACGCAGAGGGCTCCAACTGCCCCGCCACCAAGGTGTTCCAGGGCGTCCTGGAGCTGGAGGGCGTCGAGGGCCAGGAGCTGTTCTACACGCCCGAGATGGCTGACCCCAAGTCAGAACTGTTCGGGGAGACAGCCAGGAGCATTGAGAGCACCCTGGACGACCTCTTCCGGAATTCAGACGTCAAGAAGGATTTCCGGAGTGTCCGCTTGCGGGACCTGGGGCCCGGCAAATCCGTCCGCGCCATTGTGGATGTGCACTTTGACCCCACCACAGCCTTCAGGGCACCCGACGTGGCCCGGGCCCTGCTCCGGCAGATCCAGGTGTCCAGGCGCCGGTCCTTGGGGGTGAGGCGGCCGCTGCAGGAGCACGTGCGATTTATGGACTTTGACTGGTTTCCTGCGTTTATCACGGGGGCCACGTCAGGAGCCATTGCTGCGGGAGCCACGGCCAGAGCCACCACTGCATCGCGCCTGCCGTCCTCTGCTGTGACCCCTCGGGCCCCGCACCCCAGTCACACAAGCCAGCCCGTTGCCAAGACCACGGCAGCCCCCACCACACGTCGGCCCCCCACCACTGCCCCCAGCCGTGTGCCCGGACGTCGGCCCCCGGCCCCCCAGCAGCCTCCAAAGCCCTGTGACTCACAGCCCTGCTTCCACGGGGGGACCTGCCAGGACTGGGCATTGGGCGGGGGCTTCACCTGCAGCTGCCCGGCAGGCAGGGGAGGCGCCGTCTGTGAGAAGGTGCTTGGCGCCCCTGTGCCGGCCTTCGAGGGCCGCTCCTTCCTGGCCTTCCCCACCCTCCGCGCCTACCACACGCTGCGCCTGGCACTGGAATTCCGGGCGCTGGAGCCTCAGGGGCTGCTGCTGTACAATGGCAACGCCCGGGGCAAGGACTTCCTGGCATTGGCGCTGCTAGATGGCCGCGTGCAGCTCAGGTTTGACACAGGTTCGGGGCCGGCGGTGCTGACCAGTGCCGTGCCGGTAGAGCCGGGCCAGTGGCACCGCCTGGAGCTGTCCCGGCACTGGCGCCGGGGCACCCTCTCGGTGGATGGTGAGACCCCTGTTCTGGGCGAGAGTCCCAGTGGCACCGACGGCCTCAACCTGGACACAGACCTCTTTGTGGGCGGCGTACCCGAGGACCAGGCTGCCGTGGCGCTGGAGCGGACCTTCGTGGGCGCCGGCCTGAGGGGGTGCATCCGTTTGCTGGACGTCAACAACCAGCGCCTGGAGCTTGGCATTGGGCCGGGGGCTGCCACCCGAGGCTCTGGCGTGGGCGAGTGCGGGGACCACCCCTGCCTGCCCAACCCCTGCCATGGCGGGGCCCCATGCCAGAACCTGGAGGCTGGAAGGTTCCATTGCCAGTGCCCGCCCGGCCGCGTCGGACCAACCTGTGCCGATGAGAAGAGCCCCTGCCAGCCCAACCCCTGCCATGGGGCGGCGCCCTGCCGTGTGCTGCCCGAGGGTGGTGCTCAGTGCGAGTGCCCCCTGGGGCGTGAGGGCACCTTCTGCCAGACAGCCTCGGGGCAGGACGGCTCTGGGCCCTTCCTGGCTGACTTCAACGGCTTCTCCCACCTGGAGCTGAGAGGCCTGCACACCATTGCACGGGACCTGGGGGAGAAGATGGCGCTGGAGGCCGTGTTCCTGGCACGAGGCCCCAGCGGCCTCCTGCTCTACAACGGGCAGAAGACGGACGGCAAGGGGGACTTCGTGTCGCTGGCACTGCGGGACCGCCGCCTGGAGTTCCGCTACGACCTGGGCAAGGGGGCAGCGGTCATCAGGAGCAGGGAGCCAGTCACCCTGGGAGCCTGGACCAGGGTCTCACTGGAGCGAAACGGCCGCAAGGGTGCCCTGCGTGTGGGCGACGGCCCCCGTGTGTTGGGGGAGTCCCCGAAATCCCGCAAGGTTCCGCACACCGTCCTCAACCTGAAGGAGCCGCTCTACGTAGGGGGCGCTCCCGACTTCAGCAAGCTGGCCCGTGCTGCTGCCGTGTCCTCTGGCTTCGACGGTGCCATCCAGCTGGTCTCCCTCGGAGGCCGCCAGCTGCTGACCCCGGAGCACGTGCTGCGGCAGGTGGACGTCACGTCCTTTGCAGGTCACCCCTGCACCCGGGCCTCAGGCCACCCCTGCCTCAATGGGGCCTCCTGCGTCCCGAGGGAGGCTGCCTATGTGTGCCTGTGTCCCGGGGGATTCTCAGGACCGCACTGCGAGAAGGGGCTGGTGGAGAAGTCAGCGGGGGACGTGGATACCTTGGCCTTTGACGGGCGGACCTTTGTCGAGTACCTCAACGCTGTGACCGAGAGCGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCCTGCGCACTGAGGCCACGCAGGGGCTGGTGCTCTGGAGTGGCAAGGCCACGGAGCGGGCAGACTATGTGGCACTGGCCATTGTGGACGGGCACCTGCAACTGAGCTACAACCTGGGCTCCCAGCCCGTGGTGCTGCGTTCCACCGTGCCCGTCAACACCAACCGCTGGTTGCGGGTCGTGGCACATAGGGAGCAGAGGGAAGGTTCCCTGCAGGTGGGCAATGAGGCCCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGAGCTGGCACCAGAGCCCCGCGCCCGCTNOV60a, CG94946-01SEQ ID NO: 11742053 aaMW at 215628.0kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRTFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQPAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEAPAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGAPCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSTESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTIARDLGEKMALEAVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60b, 275631590SEQ ID NO: 1175388 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCGCCACCAAGGTGTTCCAGGGCGTCCTGGAGCTGGAGGGCGTCGAGGGCCAGGAGCTGTTCTACACGCCCGAGATGGCTGACCCCAAGTCAGAACTGTTCGGGGAGACAGCCAGGAGCATTGAGAGCACCCTGGACGACCTCTTCCGGAATTCAGACGTCAAGAAGGATTTCCGGAGTGTCCGCTTGCGGGACCTGGGGCCCGGCAAATCCGTCCGCGCCATTGTGGATGTGCACTTTGACCCCACCACAGCCTTCAGGGCACCCGACGTGGCCCGGGCCCTGCTCCGGCAGATCCAGGTGTCCAGGCGCCGGTCCTTGGGGGTGAGGCGGCCGCTGCAGGAGCACGTGCGATTTATGGACTTTGACTGGGTCGACGGCNOV60b, 275631590SEQ ID NO: 1176129 aaMW at 14652.4kDProtein SequenceTGSATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWVDGNOV60c, 275631564SEQ ID NO: 1177316 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCTGCCAGTGCAACCCCCATGGCTCTTACGGCGGCACCTGTGACCCAGCCACAGGCCAGTGCTCCTGCCGCCCAGGTGTGGGGGGCCTCAGGTGTGACCGCTGTGAGCCTGGCTTCTGGAACTTTCGAGGCATCGTCACCGATGGCCGGAGTGGCTGTACACCCTGCAGCTGTGATCCCCAAGGCGCCGTGCGGGATGACTGTGAGCAGATGACGGGGCTGTGCTCGTGTAAGCCCGGGGTGGCTGGACCCAAGTGTGGGCAGTGTCCAGACGGCCGTGCCCTGGGCCCCGCGGGCTGTGTCGACGGCNOV60c, 275631564SEQ ID NO: 1178105 aaMW at 10579.7kDProtein SequenceTGSCQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCVDGNOV60d, CG94946-02SEQ ID NO: 11794760 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 4732CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGATGCTCAACTCCAGCCTCATGCGGATCACCCTGCGGAACCTGGAGGAGGTGGAGTTCTGTGTGGAAGATAAACCCGGGACCCACTTCACTCCAGTGCCTCCGACGCCTCCTGATGCGTGCCGGGGAATGCTGTGCGGCTTCGGCGCCGTGTGCGAGCCCAACGCGGAGGGGCCGGGCCGGGCGTCCTGCGTCTGCAAGAAGAGCCCGTGCCCCAGCGTGGTGGCGCCTGTGTGTGGGTCGGACGCCTCCACCTACAGCAACGAATGCGAGCTGCAGCGGGCGCAGTGCAGCCAGCAGCGCCGCATCCGCCTGCTCAGCCGCGGGCCGTGCGGCTCGCGGGACCCCTGCTCCAACGTGACCTGCAGCTTCGGCAGCACCTGTGCGCGCTCGGCCGACGGGCTGACGGCCTCGTGCCTGTGCCCCGCGACCTGCCGTGGCGCCCCCGAGGGGACCGTCTGCGGCAGCGACGGCGCCGACTACCCCGGCGAGTGCCAGCTCCTGCGCCGCGCCTGCGCCCGCCAGGAGAATGTCTTCAAGAAGTTCGACGGCCCTTGTGACCCCTGTCAGGGCGCCCTCCCTGACCCGAGCCGCAGCTGCCGTGTGAACCCGCGCACGCGGCGCCCTGAGATGCTCCTACGGCCCGAGAGCTGCCCTGCCCGGCAGGCGCCAGTGTGTGGGGACGACGGAGTCACCTACGAAAACGACTGTGTCATGGGCCGATCGGGGGCCGCCCGGGGTCTCCTCCTGCAGAAAGTGCGCTCCGGCCAGTGCCAGGGTCGAGACCAGTGCCCGGAGCCCTGCCGGTTCAATGCCGTGTGCCTGTCCCGCCGTGGCCGTCCCCGCTGCTCCTGCGACCGCGTCACCTGTGACGGGGCCTACAGGCCCGTGTGTGCCCAGGACGGGCGCACGTATGACAGTGATTGCTGGCGGCAGCAGGCTGAGTGCCGGCAGCAGCGTGCCATCCCCAGCAAGCACCAGGGCCCGTGTGACCAGGCCCCGTCCCCATGCCTCGGGGTGCAGTGTGCATTTGGGGCGACGTGTGCTGTGAAGAACGGGCAGGCAGCGTGTGAATGCCTGCAGGCGTGCTCGAGCCTCTACGATCCTGTGTGCGGCAGCGACGGCGTCACATACGGCAGCGCGTGCGAGCTGGAGGCCACGGCCTGTACCCTCGGGCGGGAGATCCAGGTGGCGCGCAAAGGACCCTGTGACCGCTGCGGGCAGTGCCGCTTTGGAGCCCTGTGCGAGGCCGAGACCGGGCGCTGCGTGTGCCCCTCTGAATGCGTGGCTTTGGCCCAGCCCGTGTGTGGCTCCGACGGGCACACGTACCCCAGCGAGTGCATGCTGCACGTGCACGCCTGCACACACCAGATCAGCCTGCACGTGGCCTCAGCTGGACCCTGTGAGACCTGTGGAGATGCCGTGTGTGCTTTTGGGGCTGTGTGCTCCGCAGGGCAGTGTGTGTGTCCCCGGTGTGAGCACCCCCCGCCCGGCCCCGTGTGTGGCAGCGACGGTGTCACCTACGGCAGTGCCTGCGAGCTACGGGAAGCCGCCTGCCTCCAGCAGACACAGATCGAGGAGGCCCGGGCAGGGCCGTGCGAGCAGGCCGAGTGCGGTTCCGGAGGCTCTGGCTCTGGGGAGGACGGTGACTGTGAGCAGGAGCTGTGCCGGCAGCGCGGTGGCATCTGGGACGAGGACTCGGAGGACGGGCCGTGTGTCTGTGACTTCAGCTGCCAGAGTGTCCCAGGCAGCCCGGTGTGCGGCTCAGATGGGGTCACCTACAGCACCGAGTGTGAGCTGAAGAAGGCCAGGTGTGAGTCACAGCGAGGGCTCTACGTAGCGGCCCAGGGAGCCTGCCGAGGCCCCGCCTTCGCCCCGCTGCCGCCTGTGGCCCCCTTACACTGTGCCCAGACGCCCTACGGCTGCTGCCAGGACAATATCACCGCAGCCCGGGGCGTGGGCCTGGCTGGCTGCCCCAGTGCCTGCCAGTGCAACCCCCATGGCTCTTACGGCGGCACCTGTGACCCAGCCACAGGCCAGTGCTCCTGCCGCCCAGGTGTGGGGGGCCTCAGGTGTGACCGCTGTGAGCCTGGCTTCTGGAACTTTCGAGGCATCGTCACCGATGGCCGGAGTGGCTGTACACCCTGCAGCTGTGATCCCCAAGGCGCCGTGCGGGATGACTGTGAGCAGATGACGGGGCTGTGCTCGTGTAAGCCCGGGGTGGCTGGACCCAAGTGTGGGCAGTGTCCAGACGGCCGTGCCCTGGGCCCCGCGGGCTGTGAAGCTGACGCTTCTGCGCCTGCGACCTGTGCGGAGATGCGCTGTGAGTTCGGTGCGCGGTGCGTGGAGGAGTCTGGCTCAGCCCACTGTGTCTGCCCGATGCTCACCTGTCCAGAGGCCAACGCTACCAAGGTCTGTGGGTCAGATGGAGTCACATACGGCAACGAGTGTCAGCTGAAGACCATCGCCTGCCGCCAGGGCCTGCAAATCTCTATCCAGAGCCTGGGCCCGTGCCAGGAGGCTGTTGCTCCCAGCACTCACCCGACATCTGCCTCCGTGACTGTGACCACCCCAGGGCTCCTCCTGAGCCAGGCACTGCCGGCCCCCCCCGGCGCCCTCCCCCTGGCTCCCAGCAGTACCGCACACAGCCAGACCACCCCTCCGCCCTCATCGCGACCTCGGACCACTGCCAGCGTCCCCAGGACCACCGTGTGGCCCGTGCTGACGGTGCCCCCCACGGCACCCTCCCCTGCACCCAGCCTGGTGGCGTCCGCCTTTGGTGAATCTGGCAGCACTGATGGAAGCAGCGATGAGGAACTGAGCGGGGACCAGGAGGCCAGTGGGGGTGGCTCTGGGGGGCTCGAGCCCTTGGAGGGCAGCAGCGTGGCCACCCCTGGGCCACCTGTCGAGAGGGCTTCCTGCTACAACCCCTGCCATGGGGCGGCGCCCTGCCGTGTGCTGCCCGAGGGTGGTGCTCAGTGCGAGTGCCCCCTGGGGCGTGAGGGCACCTTCTGCCAGACAGCCTCGGGGCAGGACGGCTCTGGGCCCTTCCTGGCTGACTTCAACGGCTTCTCCCACCTGGAGCTGAGAGGCCTGCACACCATTGCACGGGACCTGGGGGAGAAGATGGCGCTGGAGGCCGTGTTCCTGGCACGAGGCCCCAGCGGCCTCCTGCTCTACAACGGGCAGAAGACGGACGGCAAGGGGGACTTCGTGTCGCTGGCACTGCGGGACCGCCGCCTGGAGTTCCGCTACGACCTGGGCAAGGGGGCAGCGGTCATCAGGAGCAGGGAGCCAGTCACCCTGGGAGCCTGGACCAGGGTCTCACTGGAGCGAAACGGCCGCAAGGGTGCCCTGCGTGTGGGCGACGGCCCCCGTGTGTTGGGGGAGTCCCCGAAATCCCGCAAGGTTCCGCACACCGTCCTCAACCTGAAGGAGCCGCTCTACGTAGGGGGCGCTCCCGACTTCAGCAAGCTGGCCCGTGCTGCTGCCGTGTCCTCTGGCTTCGACGGTGCCATCCAGCTGGTCTCCCTCGGAGGCCGCCAGCTGCTGACCCCGGAGCACGTGCTGCGGCAGGTGGACGTCACGTCCTTTGCAGGTCACCCCTGCACCCGGGCCTCAGGCCACCCCTGCCTCAATGGGGCCTCCTGCGTCCCGAGGGAGGCTGCCTATGTGTGCCTGTGTCCCGGGGGATTCTCAGGACCGCACTGCGAGAAGGGGCTGGTGGAGAAGTCAGCGGGGGACGTGGATACCTTGGCCTTTGACGGGCGGACCTTTGTCGAGTACCTCAACGCTGTGACCGAGAGCGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCCTGCGCACTGAGGCCACGCAGGGGCTGGTGCTCTGGAGTGGCAAGGCCACGGAGCGGGCAGACTATGTGGCACTGGCCATTGTGGACGGGCACCTGCAACTGAGCTACAACCTGGGCTCCCAGCCCGTGGTGCTGCGTTCCACCGTGCCCGTCAACACCAACCGCTGGTTGCGGGTCGTGGCACATAGGGAGCAGAGGGAAGGTTCCCTGCAGGTGGGCAATGAGGCCCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGAGCTGGCACCAGAGCCCCGCGCCCGCTNOV60d, CG94946-02SEQ ID NO: 11801565 aaMW at 163817.1kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQPRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTIARDLGEKMALEAVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTTLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALATVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60e, CG94946-03SEQ ID NO: 1181893 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 865CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGGTGCTCTGGAGTGGCAAGGCCACGGAGCGGGCAGACTATGTGGCACTGGCCATTGTGGACGGGCACCTGCAACTGAGCTACAACCTGGGCTCCCAGCCCGTGGTGCTGCGTTCCACCGTGCCCGTCAACACCAACCGCTGGTTGCGGGTCGTGGCACATAGGGAGCAGAGGGAAGGTTCCCTGCAGGTGGGCAATGAGGCCCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGAGCTGGCACCAGAGCCCCGCGCCCGCTNOV60e, CG94946-03SEQ ID NO: 1182276 aaMW at 29688.8kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60f, CG94946-04SEQ ID NO: 11831931 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 1903CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGATGCTCAACTCCAGCCTCATGCGGATCACCCTGCGGAACCTGGAGGAGGTGGAGTTCTGTGTGGAAGATAAACCCGGGACCCACTTCACTCCAGTGCCTCCGACGCCTCCTGATGCGTGCCGGGGAATGCTGTGCGGCTTCGGCGCCGTGTGCGAGCCCAACGCGGAGGGGCCGGGCCGGGCGTCCTGCGTCTGCAAGAAGAGCCCGTGCCCCAGCGTGGTGGCGCCTGTGTGTGGGTCGGACGCCTCCACCTACAGCAACGAATGCGAGCTGCAGCGGGCGCAGTGCAGCCAGCAGCGCCGCATCCGCCTGCTCAGCCGCGGGCCGTGCGGCTCGCGGGACCCCTGCTCCAACGTGACCTGCAGCTTCGGCAGCACCTGTGCGCGCTCGGCCGACGGGCTGACGGCCTCGTGCCTGTGCCCCGCGACCTGCCGTGGCGCCCCCGAGGGGACCGTCTGCGGCAGCGACGGCGCCGACTACCCCGGCGAGTGCCAGCTCCTGCGCCGCGCCTGCGCCCGCCAGGAGAATGTCTTCAAGAAGTTCGACGGCCCTTGTGACCCCTGTCAGGGCGCCCTCCCTGACCCGAGCCGCAGCTGCCGTGTGAACCCGCGCACGCGGCGCCCTGAGATGCTCCTACGGCCCGAGAGCTGCCCTGCCCGGCAGGCGCCAGTGTGTGGGGACGACGGAGTCACCTACGAAAACGACTGTGTCATGGGCCGATCGGGGGCCGCCCGGGGTCTCCTCCTGCAGAAAGTGCGCTCCGGCCAGTGCCAGGGTCGAGACCAGTGCCCGGAGCCCTGCCGGTTCAATGCCGTGTGCCTGTCCCGCCGTGGCCGTCCCCGCTGCTCCTGCGACCGCGTCACCTGTGACGGGGCCTACAGGCCCGTGTGTGCCCAGGACGGGCGCACGTATGACAGTGATTGCTGGCGGCAGCAGGCTGAGTGCCGGCAGCAGCGTGCCATCCCCAGCAAGCACCAGGGCCCGTGTGACCAGGCCCCGTCCCCATGCCTCGGGGTGCAGTGTGCATTTGGGGCGACGTGTGCTGTGAAGAACGGGCAGGCAGCGTGTGAATGCCTGCAGGCGTGCTCGAGCCTCTACGATCCTGTGTGCGGCAGCGACGGCGTCACATACGGCAGCGCGTGCGAGCTGGAGGCCACGGCCTGTACCCTCGGGCGGGAGATCCAGGTGGCGCGCAAAGGACCCTGTGACCGCTGCGGGCAGTGCCGCTTTGGAGCCCTGCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGAGCTGGCACCAGAGCCCCGCGCCCGCTNOV60f, CG94946-04SEQ ID NO: 1184622 aaMW at 66353.9kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEAIWVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60g, CG94946-05SEQ ID NO: 11854697 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 4669CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGATGCTCAACTCCAGCCTCATGCGGATCACCCTGCGGAACCTGGAGGAGGTGGAGTTCTGTGTGGAAGATAAACCCGGGACCCACTTCACTCCAGTGCCTCCGACGCCTCCTGATGCGTGCCGGGGAATGCTGTGCGGCTTCGGCGCCGTGTGCGAGCCCAACGCGGAGGGGCCGGGCCGGGCGTCCTGCGTCTGCAAGAAGAGCCCGTGCCCCAGCGTGGTGGCGCCTGTGTGTGGGTCGGACGCCTCCACCTACAGCAACGAATGCGAGCTGCAGCGGGCGCAGTGCAGCCAGCAGCGCCGCATCCGCCTGCTCAGCCGCGGGCCGTGCGGCTCGCGGGACCCCTGCTCCAACGTGACCTGCAGCTTCGGCAGCACCTGTGCGCGCTCGGCCGACGGGCTGACGGCCTCGTGCCTGTGCCCCGCGACCTGCCGTGGCGCCCCCGAGGGGACCGTCTGCGGCAGCGACGGCGCCGACTACCCCGGCGAGTGCCAGCTCCTGCGCCGCGCCTGCGCCCGCCAGGAGAATGTCTTCAAGAAGTTCGACGGCCCTTGTGACCCCTGTCAGGGCGCCCTCCCTGACCCGAGCCGCAGCTGCCGTGTGAACCCGCGCACGCGGCGCCCTGAGATGCTCCTACGCCCCGAGAGCTGCCCTGCCCGGCAGGCGCCAGTGTGTGGGGACGACGGAGTCACCTACGAAAACGACTGTGTCATGGGCCGATCGGGGGCCGCCCGGGGTCTCCTCCTGCAGAAAGTGCGCTCCGGCCAGTGCCAGGGTCGAGACCAGTGCCCGGAGCCCTGCCGGTTCAATGCCGTGTGCCTGTCCCGCCGTGGCCGTCCCCGCTGCTCCTGCGACCGCGTCACCTGTGACGGGGCCTACAGGCCCGTGTGTGCCCAGGACGGGCGCACGTATGACAGTGATTGCTGGCGGCAGCAGGCTGAGTGCCGGCAGCAGCGTGCCATCCCCAGCAAGCACCAGGGCCCGTGTGACCAGGCCCCGTCCCCATGCCTCGGGGTGCAGTGTGCATTTGGGGCGACGTGTGCTGTGAAGAACGGGCAGGCAGCGTGTGAATGCCTGCAGGCGTGCTCGAGCCTCTACGATCCTGTGTGCGGCAGCGACGGCGTCACATACGGCAGCGCGTGCGAGCTGGAGGCCACGGCCTGTACCCTCGGGCGGGAGATCCAGGTGGCGCGCAAAGGACCCTGTGACCGCTGCGGGCAGTGCCGCTTTGGAGCCCTGTGCGAGGCCGAGACCGGGCGCTGCGTGTGCCCCTCTGAATGCGTGGCTTTGGCCCAGCCCGTGTGTGGCTCCGACGGGCACACGTACCCCAGCGAGTGCATGCTGCACGTGCACGCCTGCACACACCAGATCAGCCTGCACGTGGCCTCAGCTGGACCCTGTGAGACCTGTGGAGATGCCGTGTGTGCTTTTGGGGCTGTGTGCTCCGCAGGGCAGTGTGTGTGTCCCCGGTGTGAGCACCCCCCGCCCGGCCCCGTGTGTGGCAGCGACGGTGTCACCTACGGCAGTGCCTGCGAGCTACGGGAAGCCGCCTGCCTCCAGCAGACACAGATCGAGGAGGCCCGGGCAGGGCCGTGCGAGCAGGCCGAGTGCGGTTCCGGAGGCTCTGGCTCTGGGGAGGACGGTGACTGTGAGCAGGAGCTGTGCCGGCAGCGCGGTGGCATCTGGGACGAGGACTCGGAGGACGGGCCGTGTGTCTGTGACTTCAGCTGCCAGAGTGTCCCAGGCAGCCCGGTGTGCGGCTCAGATGGGGTCACCTACAGCACCGAGTGTGAGCTGAAGAAGGCCAGGTGTGAGTCACAGCGAGGGCTCTACGTAGCGGCCCAGGGAGCCTGCCGAGGCCCCGCCTTCGCCCCGCTGCCGCCTGTGGCCCCCTTACACTGTGCCCAGACGCCCTACGGCTGCTGCCAGGACAATATCACCGCAGCCCGGGGCGTGGGCCTGGCTGGCTGCCCCAGTGCCTGCCAGTGCAACCCCCATGGCTCTTACGGCGGCACCTGTGACCCAGCCACAGGCCAGTGCTCCTGCCGCCCAGGTGTGGGGGGCCTCAGGTGTGACCGCTGTGAGCCTGGCTTCTGGAACTTTCGAGGCATCGTCACCGATGGCCGGAGTGGCTGTACACCCTGCAGCTGTGATCCCCAAGGCGCCGTGCGGGATGACTGTGAGCAGATGACGGGGCTGTGCTCGTGTAAGCCCGGGGTGGCTGGACCCAAGTGTGGGCAGTGTCCAGACGGCCGTGCCCTGGGCCCCGCGGGCTGTGAAGCTGACGCTTCTGCGCCTGCGACCTGTGCGGAGATGCGCTGTGAGTTCGGTGCGCGGTGCGTGGAGGAGTCTGGCTCAGCCCACTGTGTCTGCCCGATGCTCACCTGTCCAGAGGCCAACGCTACCAAGGTCTGTGGGTCAGATGGAGTCACATACGGCAACGAGTGTCAGCTGAAGACCATCGCCTGCCGCCAGGGCCTGCAAATCTCTATCCAGAGCCTGGGCCCGTGCCAGGAGGCTGTTGCTCCCAGCACTCACCCGACATCTGCCTCCGTGACTGTGACCACCCCAGGGCTCCTCCTGAGCCAGGCACTGCCGGCCCCCCCCGGCGCCCTCCCCCTGGCTCCCAGCAGTACCGCACACAGCCAGACCACCCCTCCGCCCTCATCGCGACCTCGGACCACTGCCAGCGTCCCCAGGACCACCGTGTGGCCCGTGCTGACGGTGCCCCCCACGGCACCCTCCCCTGCACCCAGCCTGGTGGCGTCCGCCTTTGGTGAATCTGGCAGCACTGATGGAAGCAGCGATGAGGAACTGAGCGGGGACCAGGAGGCCAGTGGGGGTGGCTCTGGGGGGCTCGAGCCCTTGGAGGGCAGCAGCGTGGCCACCCCTGGGCCACCTGTCGAGAGGGCTTCCTGCTACAACTCCGCGTTGGGCTGCTGCTCTGATGGGAAGACGCCCTCGCTGGACGCAGAGGGCTCCAACTGCCCCGCCACCAAGGTGTTCCAGGGCGTCCTGGAGCTGGAGGGCGTCGAGGGCCAGGAGCTGTTCTACACGCCCGAGATGGCTGACCCCAAGTCAGAACTGTTCGGGGAGACAGCCAGGAGCATTGAGAGCACCCTGGACGACCTCTTCCGGAATTCAGACGTCAAGAAGGATTTCCGGAGTGTCCGCTTGCGGGACCTGGGGCCCGGCAAATCCGTCCGCGCCATTGTGGATGTGCACTTTGACCCCACCACAGCCTTCAGGGCACCCGACGTGGCCCGGGCCCTGCTCCGGCAGATCCAGGTGTCCAGGCGCCGGTCCTTGGGGGTGAGGCGGCCGCTGCAGGAGCACGTGCGATTTATGGACTTTGACTGGTTTCCTGCGTTTATCACGGGGGCCACGTCAGGAGCCATTGCTGCGGGAGCCACGGCCAGAGCCACCACTGCATCGCGCCTGCCGTCCTCTGCTGTGACCCCTCGGGCCCCGCACCCCAGTCACACAAGCCAGCCCGTTGCCAAGACCACGGCAGCCCCCACCACACGTCGGCCCCCCACCACTGCCCCCAGCCGTGTGCCCGGACGTCGGCCCCCGGCCTCCTGCGTCCCGAGGGAGGCTGCCTATGTGTGCCTGTGTCCCGGGGGATTCTCAGGACCGCACTGCGAGAAGGGGCTGGTGGAGAAGTCAGCGGGGGACGTGGATACCTTGGCCTTTGACGGGCGGACCTTTGTCGAGTACCTCAACGCTGTGACCGAGAGCGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCCTGCGCACTGAGGCCACGCAGGGGCTGGTGCTCTGGAGTGGCAAGGCCACGGAGCGGGCAGACTATGTGGCACTGGCCATTGTGGACGGGCACCTGCAACTGAGCTACAACCTGGGCTCCCAGCCCGTGGTGCTGCGTTCCACCGTGCCCGTCAACACCAACCGCTGGTTGCGGGTCGTGGCACATAGGGAGCAGAGGGAAGGTTCCCTGCAGGTGGGCAATGAGGCCCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGACCGCTNOV60g, CG94946-05SEQ ID NO: 11861544 aaMW at 162003.7kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERAIERREEEAINVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGPASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCCRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGPRPPASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60h, CG94946-06SEQ ID NO: 11876494 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 6466CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGATGCTCAACTCCAGCCTCATGCGGATCACCCTGCGGAACCTGGAGGAGGTGGAGTTCTGTGTGGAAGATAAACCCGGGACCCACTTCACTCCAGTGCCTCCGACGCCTCCTGATGCGTGCCGGGGAATGCTGTGCGGCTTCGGCGCCGTGTGCGAGCCCAACGCGGAGGGGCCGGGCCGGGCGTCCTGCGTCTGCAAGAAGAGCCCGTGCCCCAGCGTGGTGGCGCCTGTGTGTGGGTCGGACGCCTCCACCTACAGCAACGAATGCGAGCTGCAGCGGGCGCAGTGCAGCCAGCAGCGCCGCATCCGCCTGCTCAGCCGCGGGCCGTGCGGCTCGCGGGACCCCTGCTCCAACGTGACCTGCAGCTTCGGCAGCACCTGTGCGCGCTCGGCCGACGGGCTGACGGCCTCGTGCCTGTGCCCCGCGACCTGCCGTGGCGCCCCCGAGGGGACCGTCTGCGGCAGCGACGGCGCCGACTACCCCGGCGAGTGCCAGCTCCTGCGCCGCGCCTGCGCCCGCCAGGAGAATGTCTTCAAGAAGTTCGACGGCCCTTGTGACCCCTGTCAGGGCGCCCTCCCTGACCCGAGCCGCAGCTGCCGTGTGAACCCGCGCACGCGGCGCCCTGAGATGCTCCTACGGCCCGAGAGCTGCCCTGCCCGGCAGGCGCCAGTGTGTGGGGACGACGGAGTCACCTACGAAAACGACTGTGTCATGGGCCGATCGGGGGCCGCCCGGGGTCTCCTCCTGCAGAAAGTGCGCTCCGGCCAGTGCCAGGGTCGAGACCAGTGCCCGGAGCCCTGCCGGTTCAATGCCGTGTGCCTGTCCCGCCGTGGCCGTCCCCGCTGCTCCTGCGACCGCGTCACCTGTGACGGGGCCTACAGGCCCGTGTGTGCCCAGGACGGGCGCACGTATGACAGTGATTGCTGGCGGCAGCAGGCTGAGTGCCGGCAGCAGCGTGCCATCCCCAGCAAGCACCAGGGCCCGTGTGACCAGGCCCCGTCCCCATGCCTCGGGGTGCAGTGTGCATTTGGGGCGACGTGTGCTGTGAAGAACGGGCAGGCAGCGTGTGAATGCCTGCAGGCGTGCTCGAGCCTCTACGATCCTGTGTGCGGCAGCGACGGCGTCACATACGGCAGCGCGTGCGAGCTGGAGGCCACGGCCTGTACCCTCGGGCGGGAGATCCAGGTGGCGCGCAAAGGACCCTGTGACCGCTGCGGGCAGTGCCGCTTTGGAGCCCTGTGCGAGGCCGAGACCGGGCGCTGCGTGTGCCCCTCTGAATGCGTGGCTTTGGCCCAGCCCGTGTGTGGCTCCGACGGGCACACGTACCCCAGCGAGTGCATGCTGCACGTGCACGCCTGCACACACCAGATCAGCCTGCACGTGGCCTCAGCTGGACCCTGTGAGACCTGTGGAGATGCCGTGTGTGCTTTTGGGGCTGTGTGCTCCGCAGGGCAGTGTGTGTGTCCCCGGTGTGAGCACCCCCCGCCCGGCCCCGTGTGTGGCAGCGACGGTGTCACCTACGGCAGTGCCTGCGAGCTACGGGAAGCCGCCTGCCTCCAGCAGACACAGATCGAGGAGGCCCGGGCAGGGCCGTGCGAGCAGGCCGAGTGCGGTTCCGGAGGCTCTGGCTCTGGGGAGGACGGTGACTGTGAGCAGGAGCTGTGCCGGCAGCGCGGTGGCATCTGGGACGAGGACTCGGAGGACGGGCCGTGTGTCTGTGACTTCAGCTGCCAGAGTGTCCCAGGCAGCCCGGTGTGCGGCTCAGATGGGGTCACCTACAGCACCGAGTGTGAGCTGAAGAAGGCCAGGTGTGAGTCACAGCGAGGGCTCTACGTAGCGGCCCAGGGAGCCTGCCGAGGCCCCGCCTTCGCCCCGCTGCCGCCTGTGGCCCCCTTACACTGTGCCCAGACGCCCTACGGCTGCTGCCAGGACAATATCACCGCAGCCCGGGGCGTGGGCCTGGCTGGCTGCCCCAGTGCCTGCCAGTGCAACCCCCATGGCTCTTACGGCGGCACCTGTGACCCAGCCACAGGCCAGTGCTCCTGCCGCCCAGGTGTGGGGGGCCTCAGGTGTGACCGCTGTGAGCCTGGCTTCTGGAACTTTCGAGGCATCGTCACCGATGGCCGGAGTGGCTGTACACCCTGCAGCTGTGATCCCCAAGGCGCCGTGCGGGATGACTGTGAGCAGATGACGGGGCTGTGCTCGTGTAAGCCCGGGGTGGCTGGACCCAAGTGTGGGCAGTGTCCAGACGGCCGTGCCCTGGGCCCCGCGGGCTGTGAAGCTGACGCTTCTGCGCCTGCGACCTGTGCGGAGATGCGCTGTGAGTTCGGTGCGCGGTGCGTGGAGGAGTCTGGCTCAGCCCACTGTGTCTGCCCGATGCTCACCTGTCCAGAGGCCAACGCTACCAAGGTCTGTGGGTCAGATGGAGTCACATACGGCAACGAGTGTCAGCTGAAGACCATCGCCTGCCGCCAGGGCCTGCAAATCTCTATCCAGAGCCTGGGCCCGTGCCAGGAGGCTGTTGCTCCCAGCACTCACCCGACATCTGCCTCCGTGACTGTGACCACCCCAGGGCTCCTCCTGAGCCAGGCACTGCCGGCCCCCCCCGGCGCCCTCCCCCTGGCTCCCAGCAGTACCGCACACAGCCAGACCACCCCTCCGCCCTCATCGCGACCTCGGACCACTGCCAGCGTCCCCAGGACCACCGTGTGGCCCGTGCTGACGGTGCCCCCCACGGCACCCTCCCCTGCACCCAGCCTGGTGGCGTCCGCCTTTGGTGAATCTGGCAGCACTGATGGAAGCAGCGATGAGGAACTGAGCGGGGACCAGGAGGCCAGTGGGGGTGGCTCTGGGGGGCTCGAGCCCTTGGAGGGCAGCAGCGTGGCCACCCCTGGGCCACCTGTCGAGAGGGCTTCCTGCTACAACTCCGCGTTGGGCTGCTGCTCTGATGGGAAGACGCCCTCGCTGGACGCAGAGGGCTCCAACTGCCCCGCCACCAAGGTGTTCCAGGGCGTCCTGGAGCTGGAGGGCGTCGAGGGCCAGGAGCTGTTCTACACGCCCGAGATGGCTGACCCCAAGTCAGAACTGTTCGGGGAGACAGCCAGGAGCATTGAGAGCACCCTGGACGACCTCTTCCGGAATTCAGACGTCAAGAAGGATTTCCGGAGTGTCCGCTTGCGGGACCTGGGGCCCGGCAAATCCGTCCGCGCCATTGTGGATGTGCACTTTGACCCCACCACAGCCTTCAGGGCACCCGACGTGGCCCGGGCCCTGCTCCGGCAGATCCAGGTGTCCAGGCGCCGGTCCTTGGGGGTGAGGCGGCCGCTGCAGGAGCACGTGCGATTTATGGACTTTGACTGGTTTCCTGCGTTTATCACGGGGGCCACGTCAGGAGCCATTGCTGCGGGAGCCACGGCCAGAGCCACCACTGCATCGCGCCTGCCGTCCTCTGCTGTGACCCCTCGGGCCCCGCACCCCAGTCACACAAGCCAGCCCGTTGCCAAGACCACGGCAGCCCCCACCACACGTCGGCCCCCCACCACTGCCCCCAGCCGTGTGCCCGGACGTCGGCCCCCGGCCCCCCAGCAGCCTCCAAAGCCCTGTGACTCACAGCCCTGCTTCCACGGGGGGACCTGCCAGGACTGGGCATTGGGCGGGGGCTTCACCTGCAGCTGCCCGGCAGGCAGGGGAGGCGCCGTCTGTGAGAAGGTGCTTGGCGCCCCTGTGCCGGCCTTCGAGGGCCGCTCCTTCCTGGCCTTCCCCACCCTCCGCGCCTACCACACGCTGCGCCTGGCACTGGAATTCCGGGCGCTGGAGCCTCAGGGGCTGCTGCTGTACAATGGCAACGCCCGGGGCAAGGACTTCCTGGCATTGGCGCTGCTAGATGGCCGCGTGCAGCTCAGGTTTGACACAGGTTCGGGGCCGGCGGTGCTGACCAGTGCCGTGCCGGTAGAGCCGGGCCAGTGGCACCGCCTGGAGCTGTCCCGGCACTGGCGCCGGGGCACCCTCTCGGTGGATGGTGAGACCCCTGTTCTGGGCGAGAGTCCCAGTGGCACCGACGGCCTCAACCTGGACACAGACCTCTTTGTGGGCGGCGTACCCGAGGACCAGGCTGCCGTGGCGCTGGAGCGGACCTTCGTGGGCGCCGGCCTGAGGGGGTGCATCCGTTTGCTGGACGTCAACAACCAGCGCCTGGAGCTTGGCATTGGGCCGGGGGCTGCCACCCGAGGCTCTGGCGTGGGCGAGTGCGGGGACCACCCCTGCCTGCCCAACCCCTGCCATGGCGGGGCCCCATGCCAGAACCTGGAGGCTGGAAGGTTCCATTGCCAGTGCCCGCCCGGCCGCGTCGGACCAACCTGTGCCGATGAGAAGAGCCCCTGCCAGCCCAACCCCTGCCATGGGGCGGCGCCCTGCCGTGTGCTGCCCGAGGGTGGTGCTCAGTGCGAGTGCCCCCTGGGGCGTGAGGGCACCTTCTGCCAGACAGCCTCGGGGCAGGACGGCTCTGGGCCCTTCCTGGCTGACTTCAACGGCTTCTCCCACCTGGAGCTGAGAGGCCTGCACACCATTGCACGGGACCTGGGGGAGAAGATGGCGCTGGAGGCCGTGTTCCTGGCACGAGGCCCCAGCGGCCTCCTGCTCTACAACGGGCAGAAGACGGACGGCAAGGGGGACTTCGTGTCGCTGGCACTGCGGGACCGCCGCCTGGAGTTCCGCTACGACCTGGGCAAGGGGGCAGCGGTCATCAGGAGCAGGGAGCCAGTCACCCTGGGAGCCTGGACCAGGGTCTCACTGGAGCGAAACGGCCGCAAGGGTGCCCTGCGTGTGGGCGACGGCCCCCGTGTGTTGGGGGAGTCCCCGAAATCCCGCAAGGTTCCGCACACCGTCCTCAACCTGAAGGAGCCGCTCTACGTAGGGGGCGCTCCCGACTTCAGCAAGCTGGCCCGTGCTGCTGCCGTGTCCTCTGGCTTCGACGGTGCCATCCAGCTGGTCTCCCTCGGAGGCCGCCAGCTGCTGACCCCGGAGCACGTGCTGCGGCAGGTGGACGTCACGTCCTTTGCAGGTCACCCCTGCACCCGGGCCTCAGGCCACCCCTGCCTCAATGGGGCCTCCTGCGTCCCGAGGGAGGCTGCCTATGTGTGCCTGTGTCCCGGGGGATTCTCAGGACCGCACTGCGAGAAGGGGCTGGTGGAGAAGTCAGCGGGGGACGTGGATACCTTGGCCTTTGACGGGCGGACCTTTGTCGAGTACCTCAACGCTGTGACCGAGAGCGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCCTGCGCACTGAGGCCACGCAGGGGCTGGTGCTCTGGAGTGGCAAGGCCACGGAGCGGGCAGACTATGTGGCACTGGCCATTGTGGACGGGCACCTGCAACTGAGCTACAACCTGGGCTCCCAGCCCGTGGTGCTGCGTTCCACCGTGCCCGTCAACACCAACCGCTGGTTGCGGGTCGTGGCACATAGGGAGCAGAGGGAAGGTTCCCTGCAGGTGGGCAATGAGGCCCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGTGAGTGTTTTGGGGAGACTAGAGAGGGATGCCCAAGGGTCTCAGATATCCGAGGGACAGACTCCACCCCCCAGCGCCCACCCTTGAGTCAGGGTGCATGTGAGCCGGCGGGCTGGGCTCTCTTCTCCCGCTGTAGCCCCTGCAGTTCCCAGTGCTGTGGGGCCGGGAGGCGGGTGCCCAGGTGTGGGCCCCCTGCTGGTCACCTGCTCGTTGGGGTGCCCATCAGCATCACTGAGTCACAGCCGGGTGACTCCCACTGTCTGTGCTGCAGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGAGCTGGCACCAGAGCCCCGCGCCCGCTNOV60h, CG94946-06SEQ ID NO: 11882143 aaMW at 225054.6kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRPACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQENVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTIARDLGEKMALEAVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRIEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGECFGETREGCPRVSDIRGTDSTPQRPPLSQGACEPAGWALFSRCSPCSSQCCGAGRRVPRCGPPAGHLLVGVPISITESQPGDSHCLCCRGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60i, CG94946-07SEQ ID NO: 11895688 bpDNA SequenceORF Start: ATG at 37ORF Stop: TGA at 4735CCGGCGCGGCCCGCGCGCTCTTCCGCCGCCTCTCGCATGCGCCATGGCCGGCCGGTCCCACCCGGGCCCGCTGCGGGGCGGCCGCTGCTGCCTCTCCTTGTGGTGGCCGCGTGCGTCCTGCCCGGAGCCGGCGGGACATGCCCGGAGCGCGCGCTGGAGCGGCGCGAGGAGGAGGCGAACGTGGTGCTCACCGGGACGGTGGAGGAGATCCTCAACGTGGACCCGGTGCAGCACACGTACTCCTGCAAGGTTCGGGTCTGGCGGTACTTGAAGGGCAAAGACCTGGTGGCCCGGGAGAGCCTGCTGGACGGCGGCAACAAGGTGGTGATCAGCGGCTTTGGAGACCCCCTCATCTGTGACAACCAGGTGTCCACTGGGGACACCAGGATCTTCTTTGTGAACCCTGCACCCCCATACCTGTGGCCAGCCCACAAGAACGAGCTGATGCTCAACTCCAGCCTCATGCGGATCACCCTGCGGAACCTGGAGGAGGTGGAGTTCTGTGTGGAAGATAAACCCGGGACCCACTTCACTCCAGTGCCTCCGACGCCTCCTGATGCGTGCCGGGGAATGCTGTGCGGCTTCGGCGCCGTGTGCGAGCCCAACGCGGAGGGGCCGGGCCGGGCGTCCTGCGTCTGCAAGAAGAGCCCGTGCCCCAGCGTGGTGGCGCCTGTGTGTGGGTCGGACGCCTCCACCTACAGCAACGAATGCGAGCTGCAGCGGGCGCAGTGCAGCCAGCAGCGCCGCATCCGCCTGCTCAGCCGCGGGCCGTGCGGCTCGCGGGACCCCTGCTCCAACGTGACCTGCAGCTTCGGCAGCACCTGTGCGCGCTCGGCCGACGGGCTGACGGCCTCGTGCCTGTGCCCCGCGACCTGCCGTGGCGCCCCCGAGGGGACCGTCTGCGGCAGCGACGGCGCCGACTACCCCGGCGAGTGCCAGCTCCTGCGCCGCGCCTGCGCCCGCCAGGAGAATGTCTTCAAGAAGTTCGACGGCCCTTGTGACCCCTGTCAGGGCGCCCTCCCTGACCCGAGCCGCAGCTGCCGTGTGAACCCGCGCACGCGGCGCCCTGAGATGCGCCTACGGCCCGAGAGCTGCCCTGCCCGGCAGGCGCCAGTGTGTGGGGACGACGGAGTCACCTACGAAAACGACTGTGTCATGGGCCGATCGGGGGCCGCCCGGGGTCTCCTCCTGCAGAAAGTGCGCTCCGGCCAGTGCCAGGGTCGAGACCAGTGCCCGGAGCCCTGCCGGTTCAATGCCGTGTGCCTGTCCCGCCGTGGCCGTCCCCGCTGCTCCTGCGACCGCGTCACCTGTGACGGGGCCTACAGGCCCGTGTGTGCCCAGGACGGGCGCACGTATGACAGTGATTGCTGGCGGCAGCAGGCTGAGTGCCGGCAGCAGCGTGCCATCCCCAGCAAGCACCAGGGCCCGTGTGACCAGGCCCCGTCCCCATGCCTCGGGGTGCAGTGTGCATTTGGGGCGACGTGTGCTGTGAAGAACGGGCAGGCAGCGTGTGAATGCCTGCAGGCGTGCTCGAGCCTCTACGATCCTGTGTGCGGCAGCGACGGCGTCACATACGGCAGCGCGTGCGAGCTGGAGGCCACGGCCTGTACCCTCGGGCGGGAGATCCAGGTGGCGCGCAAAGGACCCTGTGACCGCTGCGGGCAGTGCCGCTTTGGAGCCCTGTGCGAGGCCGAGACCGGGCGCTGCGTGTGCCCCTCTGAATGCGTGGCTTTGGCCCAGCCCGTGTGTGGCTCCGACGGGCACACGTACCCCAGCGAGTGCATGCTGCACGTGCACGCCTGCACACACCAGATCAGCCTGCACGTGGCCTCAGCTGGACCCTGCGAGACCTGTGGAGATGCCGTGTGTGCTTTTGGGGCTGTGTGCTCCGCAGGGCAGTGTGTGTGTCCCCGGTGTGAGCACCCCCCGCCCGGCCCCGTGTGTGGCAGCGACGGTGTCACCTACGGCAGTGCCTGCGAGCTACGGGAAGCCGCCTGCCTCCAGCAGACACAGATCGAGGAGGCCCGGGCAGGGCCGTGCGAGCAGGCCGAGTGCGGTTCCGGAGGCTCTGGCTCTGGGGAGGACGGTGACTGTGAGCAGGAGCTGTGCCGGCAGCGCGGTGGCATCTGGGACGAGGACTCGGAGGACGGGCCGTGTGTCTGTGACTTCAGCTGCCAGAGTGTCCCAGGCAGCCCGGTGTGCGGCTCAGATGGGGTCACCTACAGCACCGAGTGTGAGCTGAAGAAGGCCAGGTGTGAGTCACAGCGAGGGCTCTACGTAGCGGCCCAGGGAGCCTGCCGAGGCCCCACCTTCGCCCCGCTGCCGCCTGTGGCCCCCTTACACTGTGCCCAGACGCCCTACGGCTGCTGCCAGGACAATATCACCGCAGCCCGGGGCGTGGGCCTGGCTGGCTGCCCCAGTGCCTGCCAGTGCAACCCCCATGGCTCTTACGGCGGCACCTGTGACCCAGCCACAGGCCAGTGCTCCTGCCGCCCAGGTGTGGGGGGCCTCAGGTGTGACCGCTGTGAGCCTGGCTTCTGGAACTTTCGAGGCATCGTCACCGATGGCCGGAGTGGCTGTACACCCTGCAGCTGTGATCCCCAAGGCGCCGTGCGGGATGACTGTGAGCAGATGACGGGGCTGTGCTCGTGTAAGCCCGGGGTGGCTGGACCCAAGTGTGGGCAGTGTCCAGACGGCCGTGCCCTGGGCCCCGCGGGCTGTGAAGCTGACGCTTCTGCGCCTGCGACCTGTGCGGAGATGCGCTGTGAGTTCGGTGCGCGGTGCGTGGAGGAGTCTGGCTCAGCCCACTGTGTCTGCCCGATGCTCACCTGTCCAGAGGCCAACGCTACCAAGGTCTGTGGGTCAGATGGAGTCACATACGGCAACGAGTGTCAGCTGAAGACCATCGCCTGCCGACGGTGTCACCTACGCCAGGGCCTGCAAATCTCTATCCAGAGCCTGGGCCCGTGCCAGGAGGCTGTTGCTCCCAGCACTCACCCGACATCTGCCTCCGTGACTGTGACCACCCCAGGGCTCCTCCTGAGCCAGGCACTGCCGGCCCCCCCCGGCGCCCTCCCCCTGGCTCCCAGCAGTACCGCACACAGCCAGACCACCCCTCCGCCCTCATCGCGACCTCGGACCACTGCCAGCGTCCCCAGGACCACCGTGTGGCCCGTGCTGACGGTGCCCCCCACGGCACCCTCCCCTGCACCCAGCCTGGTGGCGTCCGCCTTTGGTGAATCTGGCAGCACTGATGGAAGCAGCGATGAGGAACTGAGCGGGGACCAGGAGGCCAGTGGGGGTGGCTCTGGGGGGCCCGAGCCCTTGGAGGGCAGCAGCGTGGCCACCCCTGGGCCACCTGTCGAGAGGGCTTCCTGCTACAACCCCTGCCATGGGGCGGCGCCCTGCCGTGTGCTGCCCGAGGGTGGTGCTCAGTGCGAGTGCCCCCTGGGGCGTGAGGGCACCTTCTGCCAGACAGCCTCGGGGCAGGACGGCTCTGGGCCCTTCCTGGCTGACTTCAACGGCTTCTCCCACCTGGAGCTGAGAGGCCTGCACACCTTTGCACGGGACCTGGGGGAGAAGATGGCGCTGGAGGTCGTGTTCCTGGCACGAGGCCCCAGCGGCCTCCTGCTCTACAACGGGCAGAAGACGGACGGCAAGGGGGACTTCGTGTCGCTGGCACTGCGGGACCGCCGCCTGGAGTTCCGCTACGACCTGGGCAAGGGGGCAGCGGTCATCAGGAGCAGGGAGCCAGTCACCCTGGGAGCCTGGACCAGGGTCTCACTGGAGCGAAACGGCCGCAAGGGTGCCCTGCGTGTGGGCGACGGCCCCCGTGTGTTGGGGGAGTCCCCGGTTCCGCACACCGTCCTCAACCTGAAGGAGCCGCTCTACGTAGGGGGCGCTCCCGACTTCAGCAAGCTGGCCCGTGCTGCTGCCGTGTCCTCTGGCTTCGACGGTGCCATCCAGCTGGTCTCCCTCGGAGGCCGCCAGCTGCTGACCCCGGAGCACGTGCTGCGGCAGGTGGACGTCACGTCCTTTGCAGGTCACCCCTGCACCCGGGCCTCAGGCCACCCCTGCCTCAATGGGGCCTCCTGCGTCCCGAGGGAGGCTGCCTATGTGTGCCTGTGTCCCGGGGGATTCTCAGGACCGCACTGCGAGAAGGGGCTGGTGGAGAAGTCAGCGGGGGACGTGGATACCTTGGCCTTTGACGGGCGGACCTTTGTCGAGTACCTCAACGCTGTGACCGAGAGCGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCCTGCGCACTGAGGCCACGCAGGGGCTGGTGCTCTGGAGTGGCAAGGCCACGGAGCGGGCAGACTATGTGGCACTGGCCATTGTGGACGGGCACCTGCAACTGAGCTACAACCTGGGCTCCCAGCCCGTGGTGCTGCGTTCCACCGTGCCCGTCAACACCAACCGCTGGTTGCGGGTCGTGGCACATAGGGAGCAGAGGGAAGGTTCCCTGCAGGTGGGCAATGAGGCCCCTGTGACCGGCTCCTCCCCGCTGGGCGCCACGCAGCTGGACACTGATGGAGCCCTGTGGCTTGGGGGCCTGCCGGAGCTGCCCGTGGGCCCAGCACTGCCCAAGGCCTACGGCACAGGCTTTGTGGGCTGCTTGCGGGATGTGGTGGTGGGCCGGCACCCGCTGCACCTGCTGGAGGACGCCGTCACCAAGCCAGAGCTGCGGCCCTGCCCCACCCCATGAGCTGGCACCAGAGCCCCGCGCCCGCTGTAATTATTTTCTATTTTTGTAAACTTGTTGCTTTTTGATATGATTTTCTTGCCTGAGTGTTGGCCGGAGGGACTGCTGGCCCGGCCTCCCTTCCGTCCAGGCAGCCGTGCTGCAGACAGACCTAGTGCTGAGGGATGGACAGGCGAGGTGGCAGCGTGGAGGGCTCGGCGTGGATGGCAGCCTCAGGACACACACCCCTGCCTCAAGGTGCTGAGCCCCCGCCTTGCACTGCGCCTGCCCCACGGTGTCCCCGCCGGGAAGCAGCCCCGGCTCCTGAATCACCCTCGCTCCGTCAGGCGGGACTCGTGTCCCAAAAAGGAAGGGGCTGCTGAGGTCTGATGGGGCCCTTCCTCCGGGTGACCCCACAGGGCCTTTCCAAGCCCCTATTTGAGCTGCTCCTTCCTGTGTGTGCTCTGGACCCTGCCTCGGCCTCCTGCGCCAATACTGTGACTTCCAAACAATGTTACTGCTGGGCACAGCTCTGCGTTGCTCCCGTGCTGCCTGCGCCAGCCCCAGGCTGCTGAGGAGCAGAGGCCAGACCAGGGCCGATCTGGGTGTCCTGACCCTCAGCTGGCCCTGCCCAGCCACCCTGGACATGACCGTATCCCTCTGCCACACCCCAGGCCCTGCGAGGGGCTATCGAGAGGAGCTCACTGTGGGATGGGGTTGACCTCTGCCGCCTGCCTGGGTATCTGGGCCTGGCCATGGCTGTGTTCTTCATGTGTTGATTTTATTTGACCCCTGGAGTGGTGGGTCTCATCTTTCCCATCTCGCCTGAGAGCGGCTGAGGGCTGCCTCACTGCAAATCCTCCCCACAGCGTCAGTGAAAGTCGTCCTTGTCTCAGAATGACCAGGGGCCAGCCAGTGTCTGACCAAGGTCAAGGGGCAGGTGCAGAGGTGGCAGGGATGGCTCCGAAGCCAGAANOV60i, CG94946-07SEQ ID NO: 11901566 aaMW at 164102.4kDProtein SequenceMRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMRLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRRCHLRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGPEPLEGSSVATPGPPVERASCYNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 60B.

TABLE 60BComparison of the NOV60 protein sequences.NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g MRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERALERREEEAINVLTGTVEEILNNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g VDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g VNPAPPYLWPAHKNELMLNSSLNRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g CGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g LSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g QLLRPACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g CGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCNOV60h ------------------------------------------------------------NOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ----------------------MRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g DRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGNOV60h ------------------------------------------------------------NOV60i ----------------------MRHGRPVPPGPAAGRPLLPLLVVAACVLPGAGGTCPERNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVINOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRNOV60h ------------------------------------------------------------NOV60i ALERREEEAIWVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVINOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d SGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVENOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g CGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASANOV60h ------------------------------------------------------------NOV60i SGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVENOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d DKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDANOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g GPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQINOV60h ------------------------------------------------------------NOV60i DKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDANOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d STYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPANOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g EEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPNOV60h ------------------------------------------------------------NOV60i STYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPANOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d TCRGAPEGTVCGSDGADYPGECQLLREACARQENVFKKFDGPCDPCQGALPDPSRSCRVNNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g VCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNOV60h ------------------------------------------------------------NOV60i TCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d PRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g NITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGINOV60h ------------------------------------------------------------NOV60i PRTRRPEMRLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d CPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g VTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASNOV60h ------------------------------------------------------------NOV60i CPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d SKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACENOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g APATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRNOV60h ------------------------------------------------------------NOV60i SKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACENOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d LEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g QGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTNOV60h ------------------------------------------------------------NOV60i LEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYNOV60a --------------------------------MRHGRPVPPGPAAGRPLLPLLVVAACVLNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d PSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSNOV60e --------------------------------MRHGRPVPPGPAAGRPLLPLLVVAACVLNOV60f --------------------------------MRHGRPVPPGPAAGRPLLPLLVVAACVLNOV60g TPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQENOV60h --------------------------------MRHGRPVPPGPAAGRPLLPLLVVAACVLNOV60i PSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSNOV60a PGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESNOV60b --------------------------------------------------TGSATKVFQGNOV60c ------------------------------------------------------------NOV60d DGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWNOV60e PGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESNOV60f PGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESNOV60g ASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGNOV60h PGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESNOV60i DGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWNOV60a LLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNOV60b VLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLG-NOV60c ------------------------------------------------------------NOV60d DEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPANOV60e LLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELVLWSG-------NOV60f LLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNOV60g VLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLG-NOV60h LLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNOV60i DEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTNOV60a NLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSNOV60b --------------------------------PGKSVRAIVDVHFDP-------------NOV60c -----------------------------------TGSCQCNPHG---------------NOV60d FAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHG---------------NOV60e ---------------K------------ATERADYVALAIVDGHLQ--------------NOV60f NLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSNOV60g --------------------------------PGKSVRAIVDVHFDP-------------NOV60h NLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSNOV60i FAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHG---------------NOV60a VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADNOV60b ----------TTAFRAPDVARALLRQIQVS------------------------------NOV60c -----------SYGGTCDPATGQCS-----------------------------------NOV60d -----------SYGGTCDPATGQCS-----------------------------------NOV60e ----------LSYNLGSQPVVLRST-----------------------------------NOV60f VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADNOV60g ----------TTAFRAPDVARALLRQIQVS------------------------------NOV60h VVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADNOV60i -----------SYGGTCDPATGQCS-----------------------------------NOV60a GLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRPACARQENVFKKFDGPCDPCQGALNOV60b ------------RRRSLGVRRP-----------------LQEHVRFMDFDWVDG------NOV60c -----------CRPGVGGLRCD------------------RCEPGFWNFRG---------NOV60d -----------CRPGVGGLRCD------------------RCEPGFWNFRG---------NOV60e -------------VPVNTNRWL------------------RVVAHREQREG---------NOV60f GLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALNOV60g ------------RRRSLGVRRP-----------------LQEHVRFMDFDWFPAFITGATNOV60h GLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALNOV60i -----------CRPGVGGLRCD------------------RCEPGFWNFRG---------NOV60a PDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVNOV60b ------------------------------------------------------------NOV60c -------------------------------IVTD-----------GRS-----------NOV60d -------------------------------IVTD-----------GRS-----------NOV60e -----------------------------SLQVGN-------------------------NOV60f PDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVNOV60g SGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRNOV60h PDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVNOV60i -------------------------------IVTD-----------GRS-----------NOV60a RSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQNOV60b ------------------------------------------------------------NOV60c -------------------------GCTPCSCD---PQGAVRDDCEQ-------------NOV60d -------------------------GCTPCSCD---PQGAVRDDCEQ-------------NOV60e---------------------------EAPVTGS--SPLGATQLDTDG-------------NOV60f RSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQNOV60g RPPASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKNOV60h RSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQNOV60i -------------------------GCTPCSCD---PQGAVRDDCEQ-------------NOV60a AECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSNOV60b ------------------------------------------------------------NOV60c ------------------------------MTGLCSCKPG----------------VAG-NOV60d ------------------------------MTGLCSCKPG----------------VAG-NOV60e ---------------------------ALWLGGLPELPVG--------------------NOV60f AECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSNOV60g ALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPNOV60h AECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSNOV60i ------------------------------MTGLCSCKPG----------------VAG-NOV60a DGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQNOV60b ------------------------------------------------------------NOV60c ------------------------------PKCGQC------------------------NOV60d ------------------------------PKCGQC------------------------NOV60e ------------------------------------------------------------NOV60f DGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRF----------------------NOV60g VNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPNOV60h DGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQNOV60i ------------------------------PKCGQC------------------------NOV60a PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCENOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g KAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP---------------------NOV60h PVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCENOV60i ------------------------------------------------------------NOV60a HPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h HPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQNOV60i ------------------------------------------------------------NOV60a ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVNOV60i ------------------------------------------------------------NOV60a AAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h AAQGACRGPAFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTNOV60i ------------------------------------------------------------NOV60a CDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h CDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGNOV60i ------------------------------------------------------------NOV60a LCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h LCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPNOV60i ------------------------------------------------------------NOV60a MLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h MLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVNOV60i ------------------------------------------------------------NOV60a TVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h TVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTNOV60i ------------------------------------------------------------NOV60a APSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVEEASNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h APSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASNOV60i ------------------------------------------------------------NOV60a CYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGENOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h CYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGENOV60i ------------------------------------------------------------NOV60a TARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVEAIVDVHFDPTTAFRAPDVAEALLNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h TARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVEAIVDVHFDPTTAFRAPDVARALLNOV60i ------------------------------------------------------------NOV60a RQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h RQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVNOV60i ------------------------------------------------------------NOV60a TPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h TPRAPHPSHTSQPVAXTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCNOV60i ------------------------------------------------------------NOV60a QDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALENOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h QDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALENOV60i ------------------------------------------------------------NOV60a PQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h PQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRNOV60i ------------------------------------------------------------NOV60a RGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h RGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDNOV60i ------------------------------------------------------------NOV60a VNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h VNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTNOV60i ------------------------------------------------------------NOV60a CADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h CADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSNOV60i ------------------------------------------------------------NOV60a HLELRGLHTIARDLGEKMALEAVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h HLELRGLHTIARDLGEKMALEAVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYNOV60i ------------------------------------------------------------NOV60a DLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h DLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKNOV60i ------------------------------------------------------------NOV60a EPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEBVLRQVDVTSFAGHPCTRNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h EPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRNOV60i ------------------------------------------------------------NOV60a ASGHPCLNGASCVPREAAYSICLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNANOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNANOV60i ------------------------------------------------------------NOV60a VTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVNOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h VTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVNOV60i ------------------------------------------------------------NOV60a LRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWL-------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f -----------------------------GALPVTGSSPLGATQLDTDGALWL-------NOV60g ------------------------------------------------------------NOV60h LRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGECFGETNOV60i ------------------------------------------------------------NOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ------------------------------------------------------------NOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h REGCPRVSDIRGTDSTPQRPPLSQGACEPAGWALFSRCSPCSSQCCGAGRRVPRCGPPAGNOV60i ------------------------------------------------------------NOV60a -----------------------GGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLNOV60b ------------------------------------------------------------NOV60c -----------------------------------PDGRALGPAGCVDG-----------NOV60d -----------------------------------PDGRALGPAGCEADASAPATCAEMRNOV60e --------------------------------PALPKAYGTGFVGCLRDVVVGRHPLHLLNOV60f -----------------------GGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLNOV60g ------------------------------------------------------------NOV60h HLLVGVPISITESQPGDSHCLCCRGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLNOV60i -----------------------------------PDGRALGPAGCEADASAPATCAENRNOV60a EDAVTKPELRPCPTP---------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d CEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSNOV60e EDAVTKPELRPCPTP---------------------------------------------NOV60f EDAVTKPELRPCPTP---------------------------------------------NOV60g ------------------------------------------------------------NOV60h EDAVTKPELRPCPTP---------------------------------------------NOV60i CEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRRCHLRQGLQNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d LGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i ISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d TTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i SSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d EPLEGSSVATPGPPVERASCYNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i GSGGPEPLEGSSVATPGPPVERASCYNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d GPFLADFNGFSHLELRGLHTIARDLGEKMALEAVFLARGPSGLLLYNGQKTDGKGDFVSLNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i GQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d ALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i DFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d RKVPHTVLNLKEPLYVGGAPDFSKLAPAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i ESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d VTSFAGHPCTPASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i DVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLANOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d DGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i FDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLNOV60a ------------------------------------------------------------NOV60b ------------------------------------------------------------NOV60c ------------------------------------------------------------NOV60d LSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGNOV60e ------------------------------------------------------------NOV60f ------------------------------------------------------------NOV60g ------------------------------------------------------------NOV60h ------------------------------------------------------------NOV60i QLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDNOV60a ---------------------------------------------------------NOV60b ---------------------------------------------------------NOV60c ---------------------------------------------------------NOV60d ALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP-NOV60e ---------------------------------------------------------NOV60f ---------------------------------------------------------NOV60g ---------------------------------------------------------NOV60h ---------------------------------------------------------NOV60i GALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTPNOV60a (SEQ ID NO: 1174)NOV60b (SEQ ID NO: 1176)NOV60c (SEQ ID NO: 1178)NOV60d (SEQ ID NO: 1180)NOV60e (SEQ ID NO: 1182)NOV60f (SEQ ID NO: 1184)NOV60g (SEQ ID NO: 1186)NOV60h (SEQ ID NO: 1188)NOV60i (SEQ ID NO: 1190)


Further analysis of the NOV60a protein yielded the following properties shown in Table 60C.

TABLE 60CProtein Sequence Properties NOV60aSignalP analysis:Cleavage site between residues 34 and 35PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 5; pos. chg 2; neg. chg 0H-region:length 9; peak value 3.79PSG score:−0.61GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 0.49possible cleavage site: between 33 and 34>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 17-33−4.35PERIPHERALLikelihood = 0.53 (at 609)ALOM score: −4.35 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 24Charge difference: −6.5 C(−2.0)-N(4.5)N >= C: N-terminal side will be inside>>> membrane topology: type 2 (cytoplasmic tail 1 to 17)MITDISC:discrimination of mitochondrial targeting segR content: 3Hyd Moment(75):2.17Hyd Moment(95):10.07G content:6D/E content: 1S/T content:1Score: −4.90Gavel:prediction of cleavage sites for mitochondrial preseqR-2 motif at 25 GRP|LLNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PRTRRPE (5) at 339pat7: PKSRKVP (5) at 1755bipartite: nonecontent of basic residues: 9.5%NLS Score: 0.39KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:XXRR-like motif in the N-terminus: RHGRnoneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: noneDileucine motif in the tail: nonechecking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: nuclearReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):34.8%: mitochondrial34.8%: nuclear13.0%: cytoplasmic 4.3%: extracellular, including cell wall 4.3%: vacuolar 4.3%: Golgi 4.3%: peroxisomal>> prediction for CG94946-01 is mit (k = 23)


A search of the NOV60a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 60D.

TABLE 60DGeneseq Results for NOV60aNOV60aIdentities/Protein/Organism/LengthResidues/Similarities forGeneseq[Patent #,Matchthe MatchedExpectIdentifierDate]ResiduesRegionValueABU52400Human GPCR related protein160 . . . 20531841/1894(97%)0.0NOV40a - Homo sapiens, 1931 aa. 51 . . . 19311853/1894(97%)[WO200279398-A2, 10-OCT-2002]ABP43859Human mRNA precursor - Homo137 . . . 16691530/1533(99%)0.0sapiens, 1741 aa. [WO200231111- 1 . . . 15331530/1533(99%)A2, 18-APR-2002]AAW26609Human agrin - Homo sapiens, 4921591 . . . 2053 460/471(97%)0.0aa. [WO9721811-A2, 19-JUN-1997]22 . . . 492461/471(97%)AAB93754Human protein sequence SEQ ID583 . . . 968 381/386(98%)0.0NO: 13424 - Homo sapiens, 413 aa. 1 . . . 386384/386(98%)[EP1074617-A2, 07-FEB-2001]AAY73993Human prostate tumor EST fragment1634 . . . 2053 414/420(98%)0.0derived protein #180 - Homo sapiens, 1 . . . 416414/420(98%)416 aa. [DE19820190-A1, 04-NOV-1999]


In a BLAST search of public sequence databases, the NOV60a protein was found to have homology to the proteins shown in the BLASTP data in Table 60E.

TABLE 60EPublic BLASTP Results for NOV60aNOV60aProteinResidues/Identities/AccessionMatchSimilarities for theExpectNumberProtein/Organism/LengthResiduesMatched PortionValueO00468AGRIN precursor - Homo sapiens24 . . . 2053 2022/2030(99%)0.0(Human), 2026 aa (fragment).1 . . . 20262022/2030(99%)P25304Agrin precursor - Rattus160 . . . 2053 1558/1914(81%)0.0norvegicus (Rat), 1959 aa.51 . . . 1959 1663/1914(86%)P31696Agrin precursor - Gallus gallus128 . . . 2050 1234/1970(62%)0.0(Chicken), 1955 aa.1 . . . 19521479/1970(74%)Q90404Agrin - Discopyge ommata716 . . . 2051 733/1353(54%)0.0(Electric ray), 1328 aa (fragment).1 . . . 1325932/1353(68%)Q96IC1Hypothetical protein - Homo1562 . . . 2053  486/492(98%)0.0sapiens (Human), 488 aa1 . . . 488 486/492(98%)(fragment).


PFam analysis predicts that the NOV60a protein contains the domains shown in the Table 60F.

TABLE 60FDomain Analysis of NOV60aIdentities/PfamNOV60aSimilarities forExpectDomainMatch Regionthe Matched RegionValueNtA 34 . . . 161125/135(93%)1.1e−111128/135(95%)kazal201 . . . 24625/61(41%)7.2e−1836/61(59%)kazal276 . . . 32121/62(34%)5.1e−1333/62(53%)kazal346 . . . 39318/61(30%)7.9e−1233/61(54%)kazal420 . . . 46521/61(34%)4.1e−1638/61(62%)kazal494 . . . 53824/61(39%)3.6e−1938/61(62%)kazal559 . . . 60319/61(31%)1.4e−1838/61(62%)kazal624 . . . 66826/62(42%)1.5e−1737/62(60%)kazal709 . . . 75424/62(39%)1.2e−1640/62(65%)laminin_EGF797 . . . 84828/61(46%)1.1e−2046/61(75%)laminin_EGF851 . . . 89521/59(36%)3.6e−1137/59(63%)kazal927 . . . 97325/62(40%)5.2e−1841/62(66%)SEA1134 . . . 125638/132(29%)4.6e−37111/132(84%)EGF1337 . . . 137016/47(34%)0.0005524/47(51%)laminin_G1404 . . . 153571/155(46%)7.4e−55120/155(77%)EGF1557 . . . 158916/47(34%)5.2e−0627/47(57%)EGF1596 . . . 162816/47(34%)0.0002125/47(53%)laminin_G1672 . . . 180770/155(45%)7e−52123/155(79%)EGF1826 . . . 186014/47(30%)4.3e−0725/47(53%)laminin_G1905 . . . 203659/155(38%)2e−50125/155(81%)


Example 61

The NOV61 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 61A.

TABLE 61ANOV61 Sequence AnalysisNOV61a, CG96384-01SEQ ID NO: 1191789 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 787ATGCCTTGGGTATGCCGCCCCACTGGCTGGACAAAGCGGCGCGTGGATGTGCCTGTGGGGCCTTGCCCTGGGCATCGCTGCTGCTGCCAGCGCCCTTCACCCATGCTTCAGCATGGTGCCCATACGCACTTCCTGCAGGAGTCTGCTGGATACCTGCAGCTGGAGCACAGGAGAGATTTCAGCTCTTCTGGGAGTAGGAAGCTCTCCTTTGACACTCGTTCCTTAGTGTGCTTTCTGGAAGACCATGGGTTTGCTACTCAGCAAGCAGAAATCATTGTGTCTGCATTGGTCCAGGTACTGGAGGCCAACGTGGACATCGTCTACAAAGATATGGCCACCAAGATGAAGCAGGAGATCGCTCTTCAGCAAATAATGTCTCAGACTGTGAATGTGAAAAACGATATGATTACTTTGGAGAAGAGTGAATTTTCAGCCCTCAGAGCAGATCGTGAGAAAATAAAACTCAAACTACATCAGTTAAAACAAGTAATGGATGAAGTGATTAAAGTCCGAACAGATACTAAATTAGACTTCAACCTAGAAAAGAGCAGAGTAAAAGAATTGTATTCGTTGAATGAAAGGAAGCTGCTGGAATTGAGAACAGAAATAGTGACATTGCATGCCCAGCAAGATTGGGCCGTCACCCAGAGAGATAGGAAGATAGAAACTGAGGATGCTGGCCCCAAAACCATGCTTGAGTCATACAAGCTTGATAATATTAAATATTTAGCAGGGTCTATATTTACGTGCCTAACAGTAGCTCTGGGATTTTATCACCTGTGGATCTAANOV61a, CG96384-01Protein SequenceSEQ ID NO: 1192262 aaMW at 30251.7kDMPWVCRPTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAHTHFLQESAGYLQLEHRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKLDNIKYLAGSIFTCLTVALGFYHLWINOV61b, 277580745SEQ ID NO: 11931808 bpDNA SequenceORF Start: at 2ORF Stop: end of sequenceCACCGGATCCACCATGCCTTGGGTATGCCGCCCCACTGGCTGGACAAAGCGGCGCGTGGATGTGCCTGTGGGGCCTTGCCCTGGGCATCGCTGCTGCTGCCAGCGCCCTTCACCCATGCTTCAGCATGGTGCCTATACTCACTTCCTGCAGGAGTCTGCTGGATACCTGCAGCTGGAGCACAGGAGAGATTTCAGCTCTTCTGGGAGTAGGAAGCTCTCCTTTGACACTCGTTCCTTAGTGTGCTTTCTGGAAGACCATGGGTTTGCTACTCAGCAAGCAGAAATCATTGTGTCTGCATTGGTCCAGGTACTGGAGGCCAACGTGGACATCGTCTACAAAGATATGGCCACCAAGATGAAGCAGGAGATCGCTCTTCAGCAAATAATGTCTCAGACTGTGAATGTGAAAAACGATATGATTACTTTGGAGAAGAGTGAATTTTCAGCCCTCAGAGCAGAACGTGAGAAAATAAAACTCAAACTACATCAGTTAAAACAAGTAATGGATGAAGTGATTAAAGTCCGAACAGATACTAAATTAGACTTCAACCTAGAAAAGAGCAGAGTAAAAGAATTGTATTCGTTGAATGAAAGGAAGCTGCTGGAATTGAGAACAGAAATAGTGACATTGCATGCCCAGCAAGATTGGGCCGTCACCCAGAGAGATAGGAAGATAGAAACTGAGGATGCTGGCCCCAAAACCATGCTTGAGTCATACAAGCTTGATAATATTAAATATTTAGCAGGGTCTATATTTACGTGCCTAACAGTAGCTCTGGGATTTTATCACCTGTGGATCCTCGAGGGCNOV61b, 277580745Protein SequenceSEQ ID NO: 1194269 aaMW at 30923.4kDTGSTMPWVCRPTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAYTHFLQESAGYLQLEHRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKLDNIKYLAGSIFTCLTVALGFYHLWILEGNOV61c, CG96384-02SEQ ID NO: 1195789 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAA at 787ATGCCTTGGGTATGCCGCCCCACTGGCTGGACAAAGCGGCGCGTGGATGTGCCTGTGGGGCCTTGCCCTGGGCATCGCTGCTGCTGCCAGCGCCCTTCACCCATGCTTCAGCATGGTGCCCATACGCACTTCCTGCAGGAGTCTGCTGGATACCTGCAGCTGGAGCACAGGAGAGATTTCAGCTCTTCTGGGAGTAGGAAGCTCTCCTTTGACACTCGTTCCTTAGTGTGCTTTCTGGAAGACCATGGGTTTGCTACTCAGCAAGCAGAAATCATTGTGTCTGCATTGGTCCAGGTACTGGAGGCCAACGTGGACATCGTCTACAAAGATATGGCCACCAAGATGAAGCAGGAGATCGCTCTTCAGCAAATAATGTCTCAGACTGTGAATGTGAAAAACGATATGATTACTTTGGAGAAGAGTGAATTTTCAGCCCTCAGAGCAGAACGTGAGAAAATAAAACTCAAACTACATCAGTTAAAACAAGTAATGGATGAAGTGATTAAAGTCCGAACAGATACTAAATTAGACTTCAACCTAGAAAAGAGCAGAGTAAAAGAATTGTATTCGTTGAATGAAAGGAAGCTGCTGGAATTGAGAACAGAAATAGTGACATTGCATGCCCAGCAAGATTGGGCCGTCACCCAGAGAGATAGGAAGATAGAAACTGAGGATGCTGGCCCCAAAACCATGCTTGAGTCATACAAGCTTGATAATATTAAATATTTAGCAGGGTCTATATTTACGTGCCTAACAGTAGCTCTGGGATTTTATCACCTGTGGATCTAANOV61c, CG96384-02Protein SequenceSEQ ID NO: 11961262 aaMW at 30251.7kDMPWVCRPTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAHTHFLQESAGYLQLEHRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKLDNIKYLAGSIFTCLTVALGFYHLWINOV61d, CG96384-03SEQ ID NO: 11971285 bpDNA SequenceORF Start: at 31ORF Stop: at 280GGATCCACCATGCCTTGGGTATGCCGCCCCACTGGCTGGACAAAGCGGCGCGTGGATGTGCCTGTGGGGCCTTGCCCTGGGCATCGCTGCTGCTGCCAGCGCCCTTCACCCATGCTTCAGCATGGTGCCTATACTCACTTCCTGCAGGAGTCTGCTGGATACCTGCAGCTGGAGCACAGGAGAGATTTCAGCTCTTCTGGGAGTAGGAAGCTCTCCTTTGACACTCGTTCCTTAGTGTGCTTTCTGGAAGACCATGGGTTTGCTACTCAGCAAGCAGAACTCGAGNOV61d, CG96384-03Protein SequenceSEQ ID NO: 1198183 aaMW at 9410.5kDTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAYTHFLQESAGYLQLEHRPDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAENOV61e, CG96384-04SEQ ID NO: 11991801 bpDNA SequenceORF Start: at 31ORF Stop: at 772GGATCCACCATGCCTTGGGTATGCCGCCCCACTGGCTGGACAAAGCGGCGCGTGGATGTGCCTGTGGGGCCTTGCCCTGGGCATCGCTGCTGCTGCCAGCGCCCTTCACCCATGCTTCAGCATGGTGCCTATACTCACTTCCTGCAGGAGTCTGCTGGATACCTGCAGCTGGAGCACAGGAGAGATTTCAGCTCTTCTGGGAGTAGGAAGCTCTCCTTTGACACTCGTTCCTTAGTGTGCTTTCTGGAAGACCATGGGTTTGCTACTCAGCAAGCAGAAATCATTGTGTCTGCATTGGTCCAGGTACTGGAGGCCAACGTGGACATCGTCTACAAAGATATGGCCACCAAGATGAAGCAGGAGATCGCTCTTCAGCAAATAATGTCTCAGACTGTGAATGTGAAAAACGATATGATTACTTTGGAGAAGAGTGAATTTTCAGCCCTCAGAGCAGAACGTGAGAAAATAAAACTCAAACTACATCAGTTAAACAAGTAATGGATGAAGTGATTTAAAGTCCGAACAGATACTAAATTAGACTTCAACCTAGAAAAGAGCAGAGTAAAAGAATTGTATTCGTTGAATGAAAGGAAGCTGCTGGAATTGAGAACAGAAATAGTGACATTGCATGCCCAGCAAGATTGGGCCGTCACCCAGAGAGATAGGAAGATAGAAACTGAGGATGCTGGCCCCAAAACCATGCTTGAGTCATACAAGCTTGATAATATTAAATATTTAGCAGGGTCTATATTTACGTGCCTAACAGTAGCTCTGGGATTTTATCACCTGTGGATCCTCGAGNOV61e, CG96384-04Protein SequenceSEQ ID NO: 1200247 aaMW at 28377.4kDTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAYTHFLQESAGYLQLEHRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKLDNIKYLAGSIFTCLTVA


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 61B.

TABLE 61BComparison of the NOV61 protein sequences.NOV61a----MPWVCRPTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAHTHFLQESAGYLQLEHNOV61bTGSTMPWVCRPTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAYTHFLQESAGYLQLEHNOV61c----MPWVCRPTGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAHTHFLQESAGYLQLEHNOV61d-----------TGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAYTHFLQESAGYLQLEHNOV61e-----------TGWTKRRVDVPVGPCPGHRCCCQRPSPMLQHGAYTHFLQESAGYLQLEHN0V61aRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKN0V61bRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKN0V61cRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKNOV61dRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAE--------------------------N0V61eRRDFSSSGSRKLSFDTRSLVCFLEDHGFATQQAEIIVSALVQVLEANVDIVYKDMATKMKNOV61aQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLNOV61bQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLNOV61cQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLNOV61d------------------------------------------------------------NOV61eQEIALQQIMSQTVNVKNDMITLEKSEFSALRAEREKIKLKLHQLKQVMDEVIKVRTDTKLNOV61aDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKNOV61bDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKNOV61cDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKNOV61d------------------------------------------------------------NOV61eDFNLEKSRVKELYSLNERKLLELRTEIVTLHAQQDWAVTQRDRKIETEDAGPKTMLESYKNOV61aLDNIKYLAGSIFTCLTVALGFYHLWI---NOV61bLDNIKYLAGSIFTCLTVALGFYHLWILEGNOV61cLDNIKYLAGSTFTCLTVALGFYHLWI---NOV61d-----------------------------NOV61eLDNIKYLAGSIFTCLTVA-----------NOV61a(SEQ IDNO: 1192)NOV61b(SEQ IDNO: 1194)NOV61c(SEQ IDNO: 1196)NOV61d(SEQ IDNO: 1198)NOV61e(SEQ IDNO: 1200)


Further analysis of the NOV61a protein yielded the following properties shown in Table 61C.

TABLE 61CProtein Sequence Properties NOV61aSignalP analysis:No Known Signal Sequence PredictedPSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 6; pos. chg 1; neg. chg 0H-region:length 5; peak value −14.81PSG score:−19.21GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): −11.24possible cleavage site: between 29 and 30>>> Seems to have no N-terminal signal peptideALOM:Klein et al's method for TM region allocationInit position for calculation: 1Tentative number of TMS(s) for the threshold 0.5: 2Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 91-107−2.13PERIPHERALLikelihood = 11.30 (at 68)ALOM score: −2.13 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 98Charge difference: 1.5 C(−1.0)-N(−2.5)C > N: C-terminal side will be inside>>> membrane topology: type 1b (cytoplasmic tail 91 to 262)MITDISC:discrimination of mitochondrial targeting seqR content:3Hyd Moment(75):7.07Hyd Moment(95):9.21G content:1D/E content:1S/T content:2Score: −1.63Gavel:prediction of cleavage sites for mitochondrial preseqR-10 motif at 68 RRD FSNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: nonebipartite: nonecontent of basic residues: 14.1%NLS Score: −0.47KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals: noneSKL: peroxisomal targeting signal in the C-terminus: nonePTS2: 2nd peroxisomal targeting signal: foundKIKLKLHQL at 152VAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 196checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 76.7COIL:Lupas's algorithm to detect coiled-coil regions138 E0.87139 K0.87140 S0.87141 E0.87142 F0.87143 S0.87144 A0.87145 L0.87146 R0.87147 A0.87148 E0.87149 R0.87150 E0.87151 K0.87152 I0.87153 K0.87154 L0.87155 K0.87156 L0.87157 H0.87158 Q0.87159 L0.87160 K0.87161 Q0.87162 V0.87163 M0.87164 D0.87165 E0.87166 V0.86167 I0.79168 K0.79169 V0.59170 R0.59171 T0.59172 D0.59total: 35 residuesFinal Results (k = 9/23):34.8%: nuclear21.7%: mitochondrial21.7%: cytoplasmic 8.7%: vesicles of secretory system 4.3%: vacuolar 4.3%: peroxisomal 4.3%: endoplasmic reticulum>> prediction for CG96384-01 is nuc (k = 23)


A search of the NOV61a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 61D.

TABLE 61DGeneseq Results for NOV61aNOV61aIdentities/Protein/Organism/LengthResidues/Similarities forGeneseq[Patent #,Matchthe MatchedExpectIdentifierDate]ResiduesRegionValueAAM40693Human polypeptide SEQ ID NO 5624 -45 . . . 262185/220(84%)2e−95Homo sapiens, 246 aa.27 . . . 246197/220(89%)[WO200153312-A1, 26-JUL-2001]AAM38907Human polypeptide SEQ ID NO 2052 -104 . . . 262 138/160(86%)3e−70Homo sapiens, 160 aa. 1 . . . 160145/160(90%)[WO200153312-A1, 26-JUL-2001]AAG73708Human colon cancer antigen protein142 . . . 262 110/122(90%)9e−54SEQ ID NO: 4472 - Homo sapiens, 129 8 . . . 129114/122(93%)aa. [WO200122920-A2, 05-APR-2001]ABU53059Human testes-derived protein from59 . . . 26196/204(47%)3e−48DKFZphtes3_19f19 - Homo sapiens,50 . . . 253138/204(67%)254 aa. [WO200112659-A2, 22-FEB-2001]ABB89935Human polypeptide SEQ ID NO 2311 -59 . . . 26196/204(47%)3e−48Homo sapiens, 245 aa.41 . . . 244138/204(67%)[WO200190304-A2, 29-NOV-2001]


In a BLAST search of public sequence databases, the NOV61a protein was found to have homology to the proteins shown in the BLASTP data in Table 61E.

TABLE 61EPublic BLASTP Results for NOV61aNOV61aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueQ96JS7Similar to RIKEN cDNA 6230416A0545 . . . 262186/220(84%)1e−96gene - Homo sapiens (Human), 321102 . . . 321 198/220(89%)aa (fragment).Q96AQ8Similar to RIKEN cDNA 6230416A0545 . . . 262185/220(84%)6e−95gene - Homo sapiens (Human), 359140 . . . 359 197/220(89%)aa.Q9NUI2DJ500L14.1 (Novel protein) - Homo45 . . . 262185/220(84%)6e−95sapiens (Human), 220 aa (fragment). 1 . . . 220197/220(89%)Q9CXD66230416A05Rik protein - Mus45 . . . 262163/220(74%)3e−83musculus (Mouse), 340 aa.121 . . . 340 188/220(85%)Q9GZT6MDS011 (MDS025) (Hypothetical59 . . . 26196/204(47%)9e−48protein) - Homo sapiens (Human),50 . . . 253138/204(67%)254 aa.


PFam analysis predicts that the NOV61a protein contains the domains shown in the Table 61F.

TABLE 61FDomain Analysis of NOV61aPfamNOV61aIdentities/ExpectDomainMatch RegionSimilarities forValuethe Matched Region


Example 62

The NOV62 clone was analyzed, and the nucleotide and encoded polypeptide sequences are shown in Table 62A.

TABLE 62ANOV62 Sequence AnalysisNOV62a, CG98011-01SEQ ID NO: 120112631 bpDNA SequenceORF Start: ATG at 1ORF Stop: TAG at 2629ATGATTTACATACAAGTAATTTTTCAAGTAATGACCATTGAAAAAATGTTTTCTTTTTATTTTTTAGATTATTTCTCTTTATTCAGAAGCATACAGTTGTTTGCTGATTGCAAGAAGATGTTTCTGTGGCTGTTTCTGATTTTGTCAGCCCTGATTTCTTCGACAAATGCAGATTCTGACATATCGGTGGAAATTTGCAATGTGTGTTCCTGCGTGTCAGTTGAGAATGTGCTCTATGTCAACTGTGAGAAGGTTTCAGTCTACAGACCAAACAGCTAGAAACCACCTTGGTCTAATTTTTATCACCTCAATTTCCAAAATAATTTTTTAAATATTCTGTATCCAAATACATTCTTGAATTTTTCACATGCAGTCTCCCTGCATCTGGGGAATAATAAACTGCAGAACATTGAGGGAGGAGCCTTTCTTGGGCTCAGTGCATTAAAGCAGTTGCACTTGAACAACAATGAATTAAAGATTCTCCGAGCTGACACTTTCCTTGGCATAGAGAACTTGGAGTATCTCCAGGCTGACTACAATTTAATCAAGTATATTGAACTGAGGAGCCTTCAATAAGCTCCACAAACTGAAGTTCTCATTCTTTTTGACAATCTGATTTCATTCCTTCCTGATAATATTTTCCGATTCGCATCTTTGACCCATCTGGATATACGAGGGAACAGAATCCAGAAGCTCCCTTATATCGGGGTTCTGGAACACATTGGCCGTGTCGTTGAATTGCAACTGGAACATTTACATAGGAGAAGCTATCTGTGAAACTCCCAGTGACTTATATGGAAGGCTTTTAAAAGAAACAGCTGGAAAATGGCTACACCACTCCCAATGGTCACACTACCCAAACATCTTTACACAGATTAGTAACTAAACCACCAAAAACAACAAATCCTTCCAAGATCTCTGGAATCGTTGCAGGCAAAGCCCTCTCCAACCGCAATCTCAGTCAGATTGTGTCTTACCAAACAAGGGTGCCTCCTCTAACACCTTGCCCGGCACCTTGCTTCTGCAAAACACACCCTTCAGATTTGGGACTAAGTGTGAACTGCCAAGAGAAAAATATACAGTCTATGTCTGAACTGATACCGAAACCTTTAAATGCGAAGAAGCTGCACGTCAATGGCAATAGCATCAAGGATGTGGACGTATCAGACTTCACTGACTTTGAAGGACTGGATTTGCTTCATCTAGGCAGCAATCAAATTACAGTGATTAAGGGAGACGTATTTCACAATCTCACTAATTTACGCAGGCTATATCTCAATGGCAATCAAATTGAGAGACTCTATCCTGAAATATTTTCAGGTCTTCATAACCTGCAGTATCTGTATTTGGAATACAATTTGATTAAGGAAATCTCAGCAGGCACCTTTGACTCCATGCCAAATTTGCAGTTACTGTACTTAAACAATAATCTCCTAAAGAGCCTGCCTGTTTACATCTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACAAATTCATGTACCTGCCTGTCAGTGGGGTCCTTGATCAGTTGCAATCTCTTACACAGATTGACTTGGAGGGCAACCCATGGGACTGTACTTGTGACTTGGTGGCATTAAAGCTGTGGGTGGAGAAGTTGAGCGACGGGATTGTTGTGAAAGAACTGAAATGTGAGACGCCTGTTCAGTTTGCCAACATTGAACTGAAGTCCCTCAAAAATGAAATCTTATGTCCCAAACTTTTAAATAAGCCGTCTGCACCATTCACAAGCCCTGCACCTGCCATTACATTCACCACTCCTTTGGGTCCCATTCGAAGTCCTCCTGGTGGGCCAGTGCCTCTGTCTATTTTAATCTTAAGTATCTTAGTGGTCCTCATTTTAACGGTGTTTGTTGCTTTTTGCCTTCTTGTTTTTGTCCTGCGACGCAACAAGAAACCCACAGTGAAGCACGAAGGCCTGGGGAATCCTGACTGTGGCTCCATGCAGCTGCAGCTAAGGAAGCATGACCACAAAACCAATAAAAAAGATGGACTGAGCACAGAAGCTTTCATTCCACAAACTATAGAACAGATGAGCAAGAGCCACACTTGTGGCTTGAAAGAGTCAGAAACTGGGTTCATGTTTTCAGATCCTCCAGGACAGAAAGTTGTTATGAGAAATGTGGCCGACAAGGAGAAAGATTTATTACATGTAGATACCAGGAAGAGACTGAGCACAATTGATGAGCTGGATGAATTATTCCCTAGCAGGGATTCCAATGTGTTTATTCAGAATTTTCTTGAAAGCAAAAAGGAGTATAATAGCATAGGTGTCAGTGGCTTTGAGATCCGCTATCCAGAAAAACAACCAGACAAAAAAAGTAAGAAGTCACTGATAGGTGGCAACCACAGTAAAATTGTTGTGGAACAAAGGAAGAGTGAGTATTTTGAACTGAAGGCGAAACTGCAGAGTTCCCCTGACTACCTACAGGTCCTTGAGGAGCAAACAGCTTTGAACAAGATCTAGNOV62a, CG98011-01Protein SequenceSEQ ID NO: 1202876 aaMW at 99167.6kDMIYIQVIFQVMTIEKMFSFYFLDYFSLFRSIQLFADCKKMFLWLFLILSALISSTNADSDISVEICNVCSCVSVENVLYVNCEKVSVYRPNQLKPPWSNFYHLNFQNNFLNILYPNTFLNFSHAVSLHLGNNKLQNIEGGAFLGLSALKQLHLNNNELKILRADTFLGIENLEYLQADYNLIKYIERGAFNKLHKLKVLILNDNLISFLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRVVELQLEDNPWNCSCDLLPLKAWLENMPYNIYIGEAICETPSDLYGRLLKETNKQELCPMGTGSDFDVRILPPSQLENGYTTPNGHTTQTSLHRLVTKPPKTTNPSKISGIVAGKALSNRNLSQIVSYQTRVPPLTPCPAPCFCKTHPSDLGLSVNCQEKNIQSMSELIPKPLNAKKLHVNGNSIKDVDVSDFTDFEGLDLLHLGSNQITVIKGDVFHNLTNLRPLYLNGNQIERLYPEIFSGLHNLQYLYLEYNLIKEISAGTFDSMPNLQLLYLNNNLLKSLPVYIFSGAPLAELNLRNNKFMYLPVSGVLDQLQSLTQIDLEGNPWDCTCDLVALKLWVEKLSDGIVVKELKCETPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPAITFTTPLGPIRSPPGGPVPLSILILSILVVLILTVFVAFCLLVFVLRRNKKPTVKHEGLGNPDCGSMQLQLRKHDHKTAKKDGLSTEAFIPQTIEQMSKSHTCGLKESETGFMFSDPPGQKVVMRNVADKEKDLLHVDTRKRLSTIDELDELFPSRDSNVFIQNFLESKKEYNSIGVSGFEIRYPEKQPDKKSKKSLIGGNHSKIVVEQRKSEYFELKAXLQSSPDYLQVLEEQTALNKINOV62b, 192586956SEQ ID NO: 12031800 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGATTCTGACATATCGGTGGAAATTTGCAATGTGTGTTCCTGCGTGTCAGTTGAGAATGTGCTCTATGTCAACTGTGAGAAGGTTTCAGTCTACAGACCAAATCAGCTGAAACCACCTTGGTCTAATTTTTATCACCTCAATTTCCAAAATAATTTTTTAAATATTCTGTATCCAAATACATTCTTGAATTTTTCACATGCAGTCTCCCTGCATCTGGGGAATAATAAACTGCAGAACATTGAGGGAGGAGCCTTTCTTGGGCTCAGTACATTAAAGCAGTTGCACTTGAACAACAATGAATTAAAGATTCTCCGAGCTGACACTTTCCTTGGCATAGAGAACTTGGAGTATCTCCAGGCTGACTACAATTTAATCAAGTATATTGAACGAGGAGCCTTCAATAAGCTCCACAAACTGAAAGTTCTCATTCTTAATGACAATCTGATTTCATTCCTTCCTGATAATATTTTCCGATTCGCATCTTTGACCCATCTGGATATACGAGGGAACAGAATCCAGAAGCTCCCTTATATCGGGGTTCTGGAACACATTGGTCGTGTCGTTGAATTGCAACTGGAAGATAACCCTTGGAACTGTAGCTGTGATTTATTGCCCTTAAAAGCTTGGCTGGAGAACATGCCATATAACATTTACATAGGAGAAGCTATCTGTGAAACTCCCAGTGACTTATATGGAAGGCTTTTAAAAGAAACCAACAAACAAGAGCTATGTCCCATGGGCACCGGCAGTGATTTTGACGTGCGCATCCTGCCTCCATCTCAGCTGGAAAATGGCTACACCACTCCCAATGGTCACACTACCCAAACATCTTTACACAGATTAGTAACTAAACCACCAAAAACAACAAATCCTTCCAAGATCTCTGGAATCGTTGCAGGTAAAGCCCTCTCCAACCGCAATCTCAGTCAGATTGTGTCTTACCAAACAAGGGTGCCTCCTCTAACACCTTGCCCGGCACCTTGCTTCTGCAAAACACACCCTTCAGATTTGGGACTAAGTGTGAACTGCCAAGAGAAAAATATACAGTCTATGTCTGAACTGATACCGAAACCTTTAAATGCGAAGAAGCTGCACGTCAATGGCAATAGCATCAAGGATGTGGACGTATCAGACTTCACTGACTTTGAAGGACTGGATTTGCTTCATTTAGGCAGCAATCAAATTACAGTGATTAAGGGAGACGTATTTCACAATCTCACTAATTTACGCAGGCTATATCTCAATGGCAATCAAATTGAGAGACTCTATCCTGAAATATTTTCAGGTCTTCATAACCTGCAGTATCTGTATTTGGAATACAATTTGATTAAGGAAATCTCAGCAGGCACCTTTGACTCCATGCCAAATTTGCAGTTACTGTACTTAAGCAATAATCTCCTAAAGAGCCTGCCTGTTTACATCTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACATATTCATGTACCTGCCTGTCAGTGGGGTCCTTGATCAGTTGCAATCTCTTACACAGATTGACTTGGAGGGCAGCCCATGGGACTATACTTGTGACTTGGTGGCATTAAAGCTGTGGGTGGAGAAGTTGAGCGACGGGATTGTTGTGAAAGAACTGAAATGTTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACAAATTCATGTACCTGCCTGTCAGTGGTTTAAATAAGCCGTCTGCACCATTCACAAGCCCTGCACCTGCCATTACATTCACCACTCCTTTGGGTCCCATTCGAAGTCCTCCTGGTGGGCCACTCGAGNOV62b, 192586956Protein SequenceSEQ ID NO: 12041600 aaMW at 67403.8kDGSDSDISVEICNVCSCVSVENVLYVNCEKVSVYRPNQLKPPWSNFYHLNFQNNFLNILYPNTFLNFSHAVSLHLGNNKLQNIEGGAFLGLSTLKQLHLNNNELKILRADTFLGIENLEYLQADYNLIKYIERGAFNKLHKLKVLILNDNLISFLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRVVELQLEDNPWNCSCDLLPLKAWLENMPYNIYIGEAICETPSDLYGRLLKETNKQELCPMGTGSDFDVRILPPSQLENGYTTPNGHTTQTSLHRLVTKPPKTTNPSKISGIVAGKALSNRNLSQIVSYQTRVPPLTPCPAPCFCKTHPSDLGLSVNCQEKNIQSMSELIPKPLNAKKLHVNGNSIKDVDVSDFTDFEGLDLLHLGSNQITVIKGDVFHNLTNLRRLYLNGNQIERLYPEIFSGLHNLQYLYLEYNLIKEISAGTFDSMPNLQLLYLSNNLLKSLPVYIFSGAPLARLNLRNNKFMYLPVSGVLDQLQSLTQIDLEGSPWDYTCDLVALKLWVEKLSDGIVVKELKCIETPVQFTNIELKSLKEILCPKLLNKPSAPFTSPAPAITFTTPLGPIRSPPGGPLENOV62c, 191999007SEQ ID NO: 12051800 bpDNA SequenceORF Start: at 1ORF Stop: end of sequenceGGATCCGATTCTGACATATCGGTGGAAATTTGCAATGTGTGTTCCTGCGTGTCAGTTGAGAATGTGCTCTATGTCAACTGTGAGAAGGTTTCAGTCTACAGACCAAATCAGCTGAAACCACCTTGGTCTAATTTTTATCACCTCAATTTCCAATAATTTTTTAATTTTATTCTGTATCCAAATACATTCTTGAATTTTTCACATGCAGTCTCCCTGCATCTGGGGAATAATAAACTGCAGAACATTGAGGGAGGAGCCTTTCTTGGGCTCAGTGCATTAAAGCAGTTGCACTTGAACAACAATGAATTAAAGATTCTCCGAGCTGACACTTTCCCTGGCATAGAGAACTTGGAGTATCTCCAGGCTGACTACAATTTAATCAAGTATATTGAACGAGGAGCCTTCAATAAGCTCCACAAACTGAAAGTTCTCATTCTTAATGACAATCTGATTTCATTCCTTCCTGATAATATTTTCCGATTCGCATCTTTGACCCATCTGGATATACGAGGGAACAGAATCCAGAAGCTCCCTTATATCGGGGTTCTGGAACACATTGGCCGTGTCGTTGAATTGCAACTGGAAGATAACCCTTGGAACTGTAGCTGTGATTTATTGCCCTTAAAAGCTTGGCTGGAGAACATGCCATATAACATTTACATAGGAGAAGCTATCTGTGAAACTCCCAGTGACTTATATGGAAGGCTTTTAAAAGAAACCAACAAACAAGAGCTATGTCCCATGGGCACCGGCAGTGATTTTGACGTGCGCATCCTGCCTCCATCTCAGCTGGAAAATGGCTACACCACTCCCAATGGTCACACTACCCAAACATCTTTACACAGATTAGTAACTAAACCACCAAAAACAACAAATCCTTCCAAGATCTCTGGAATCGTTGCAGGCAAAGCCCTCTCCAACCGCAATCTCAGTCAGATTGTGTCTTACCAAACAAGGGTGCCTCCTCTAACACCTTGCCCGGCACCTTGCTTCTGCAAAACACACCCTTCAGATTTGGGACTAAGTGTGAACTGCCAAGAGAAAAATATACAGTCTATGTCTGAACTGATACCGAAACCTTTAAATGCGAAGAAGCTGCACGTCAATGGCAATAGCATCAAGGATGTGGACGTATCAGACTTCACTGACTTTGAAGGACTGGATTTGCTTCATTTAGGCAGCAATCAAATTACAGTGATTAAGGGAGACGTATTTCACAATCTCACTAATTTACGCAGGCTATATCTCAATGGCAATCAAATTGAGAGACTCTATCCTGAAATATTTTCAGGTCTTCATAACCTGCAGTATCTGTATTTGGAATACAATTTGATTAAGGAAATCTCAGCAGGCACCTTTGACTCCATGCCAAATTTGCAGTTACTGTACTTAAACAATAATCTCCTAAAGAGCCTGCCTGTTTACATCTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACAAATTCATGTACCTGCCTGTCAGTGGGGTCCTTGATCAGTTGCAATCTCTTACACAGATTGACTTGGAGGGCAACCCATGGGACTGTACTTGTGACTTGGTGGCATTAAAGCTGTGGGTGGGGAAGTTGAGCGACGGGATTGTTGTGAAAGAACTGAAATGTGAGACGCCTGTTCAGTTTGCCAACATTGAACTGAAGTCCCTCAAAAATGAAATCTTATGTCCCAAACTTTTAAATAAGCCGTCTGCACCATTCACAAGCCCTGCACCTACCATTACATTCACCACTCCTTTGGGTCCCATTCGAAGTCCTCCTGGTGGGCCACTCGAGNOV62c, 191999007Protein SequenceSEQ ID NO: 1206600 aaMW at 67279.7kDGSDSDISVEICNVCSCVSVENVLYVNCEKVSVYRPNQLKPPWSNFYHLNFQNNFLNTLYPNTFLNFSHAVSLHLGNNKLQNIEGGAFLGLSALKQLHLNNNELKILRADTFPGIENLEYLQADYNLIKYIERGAFNKLHKLKVLILNDNLISFLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRVVELQLEDNPWNCSCDLLPLKAWLENMPYNIYIGEAICETPSDLYGRLLKETNKQELCPMGTGSDFDVRILPPSQLENGYTTPNGHTTQTSLHRLVTKPPKTTNPSKISGIVAGKALSNRNLSQIVSYQTRVPPLTPCPAPCFCKTHPSDLGLSVNCQEKNIQSMSELIPKPLNAKKLHVNGNSIKDVDVSDFTDFEGLDLLHLGSNQITVIKGDVFHNLTNLRRLYLNGNQIERLYPEIFSGLHNLQYLYLEYNLIKEISAGTFDSMPNLQLLYLNNNLLKSLPVYIFSGAPLARLNLRNNKFMYLPVSGVLDQLQSLTQIDLEGNPWDCTCDLVALKLWVGKLSDGIVVKELKCETPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPTITFTTPLGPIRSPPGGPLENOV62d, CG98011-02SEQ ID NO: 120712654 bpDNA SequenceORF Start: ATG at 19ORF Stop: TAG at 2647CCATGTGTTCACTATGGTATGATTTACATACAAGTAATTTTTCAAGTAATGACCATTGAAAAAATGTTTTCTTTTTATTTTTTAGATTATTTCTCTTTATTCAGAAGCATACAGTTGTTTGCTGATTGCAAGAAGATGTTTCTGTGGCTGTTTCTGATTTTGTCAGCCCTGATTTCTTCGACAAATGCAGATTCTGACATATCGGTGGAAATTTGCAATGTGTGTTCCTGCGTGTCAGTTGAGAATGTGCTCTATGTCAACTGTGAGAAGGTTTCAGTCTACAGACCAAATCAGCTGAAACCACCTTGGTCTAATTTTTATCACCTCAATTTCCAAAATAATTTTTTAAATATTCTGTATCCAAATACATTCTTGAATTTTTCACATGCAGTCTCCCTGCATCTGGGGAATAATAAACTGCAGAACATTGAGGGAGGAGCCTTTCTTGGGCTCAGTGCATTAAAGCAGTTGCACTTGAACAACAATGAATTAAAGATTCTCCGAGCTGACACTTTCCTTGGCATAGAGAACTTGGAGTATCTCCAGGCTGACTACAATTTAATCAAGTATATTGAACGAGGAGCCTTCAATAAGCTCCACAAACTGAAAGTTCTCATTCTTAATGACAATCTGATTTCATTCCTTCCTGATAATATTTTCCGATTCGCATCTTTGACCCATCTGGATATACGAGGGAACAGAATCCAGAAGCTCCCTTATATCGGGGTTCTGGAACACATTGGCCGTGTCGTTGAATTGCAACTGGAAGATAACCCTTGGAACTGTAGCTGTGATTTATTGCCCTTAAAAGCTTGGCTGGAGAACATGCCATATAACATTTACATAGGAGAAGCTATCTGTGAAACTCCCAGTGACTTATATGGAAGGCTTTTAAAGAAACCAACAAACAAGAGCTATGATCCCATGGGCACCGGCAGTGATTTTGACGTGCGCATCCTGCCTCCATCTCAGCTGGAAAATGGCTACACCACTCCCAATGGTCACACTACCCAAACATCTTTACACAGATTAGTAACTAACCACCAAAAACAACAAATCACTTCCAAGATCTCTGGAATCGTTGCAGGCAAAGCCCTCTCCAACCGCAATCTCAGTCAGATTGTGTCTTACCAAACAAGGGTGCCTCCTCTAACACCTTGCCCGGCACCTTGCTTCTGCAAAACACACCCTTCAGATTTGGGACTAAGTGTGAACTGCCAAGAGAAAATATACAGTCTATGTCTGAACTGATACCGAAACCTTTAAAATGCGAAGAAGCTGCACGTCAATGGCAATAGCATCAAGGATGTGGACGTATCAGACTTCACTGACTTTGAAGGACTGGATTTGCTTCATTTAGGCAGCAATCAAATTACAGTGATTAAGGGAGACGTATTTCACAATCTCACTAATTTACGCAGGCTATATCTCAATGGCAATCAAATTGAGAGACTCTATCCTGAAATATTTTCAGGTCTTCATAACCTGCAGTATCTGTATTTGGAATACAATTTGATTAAGGAAATCTCAGCAGGCACCTTTGACTCCATGCCAAATTTGCAGTTACTGTACTTAAACAATAATCTCCTAAAGAGCCTGCCTGTTTACATCTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACAAATTCATGTACCTGCCTGTCAGTGGGGTCCTTGATCAGTTGCAATCTCTTACACAGATTGACTTGGAGGGCAACCCATGGGACTGTACTTGTGACTTGGTGGCATTAAAGCTGTGGGTGGAGAAGTTGAGCGACGGGATTGTTGTGAAAGAACTGAAATGTGAGACGCCTGTTCAGTTTGCCAACATTGAACTGAAGTCCCTCAAAAATGAAATCTTATGTCCCAAACTTTTAAATAAGCCGTCTGCACCATTCACAAGCCCTGCACCTGCCATTACATTCACCACTCCTTTGGGTCCCATTCGAAGTCCTCCTGGTGGGCCAGTGCCTCTGTCTATTTTAATCTTAAGTATCTTAGTGGTCCTCATTTTAACGGTGTTTGTTGCTTTTTGCCTTCTTGTTTTTGTCCTGCGACGCAACAAGAAACCCACAGTGAAGCACGAAGGCCTGGGGAATCCTGACTGTGGCTCCATGCAGCTGCAGCTAAGGAAGCATGACCACAAAACCAATAAAAAAGATGGACTGAGCACAGAAGCTTTCATTCCACAAACTATAGAACAGATGAGCAAGAGCCACACTTGTGGCTTGAAAGAGTCAGAAACTGGGTTCATGTTTTCAGATCCTCCAGGACAGAAAGTTGTTATGAGAAATGTGGCCGACAAGGAGAAAGATTTATTACATGTAGATACCAGGAAGAGACTGAGCACAATTGATGAGCTGGATGAATTATTCCCTAGCAGGGATTCCAATGTGTTTATTCAGAATTTTCTTGAAAGCAAAAAGGAGTATAATAGCATAGGTGTCAGTGGCTTTGAGATCCGCTATCCAGAAAAACAACCAGACAAAAAAAGTAAGAAGTCACTGATAGGTGGCAACCACAGTAAAATTGTTGTGGAACAAAGGAAGAGTGAGTATTTTGAACTGAAGGCGAAACTGCAGAGTTCCCCTGACTACCTACAGGTCCTTGAGGAGCAAACAGCTTTGAACAAGATCTAGGTCATNOV62d, CG9801 -02Protein SequenceSEQ ID NO: 1208876 aaMW at 99167.6kDMIYIQVIFQVMTIEKMFSFYFLDYFSLFRSIQLFADCKKMFLWLFLILSALISSTNADSDISVEICNVCSCVSVENVLYVNCEKVSVYRPNQLKPPWSNFYHLNFQNNFLNILYPNTFLNFSHAVSLHLGNNKLQNIEGGAFLGLSALKQLHLNNNELKILRADTFLGIENLEYLQADYNLIKYIERGAFNKLHKLKVLILNDNLISFLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRWELQLEDNPWNCSCDLLPLKAAWLENMPYNIYIGEAICETPSDLYGRLLKETNKQELCPMGTGSDFDVRILPPSQLENGYTTPNGHTTQTSLHRLVTKPPKTTNPSKISGIVAGKALSNRNLSQIVSYQTRVPPLTPCPAPCFCKTHPSDLGLSVNCQEKNIQSMSELIPKPLNAKKLHVNGNSIKDVDVSDFTDFEGLDLLHLGSNQITVIKGDVFHNLTNLRRLYLNGNQIERLYPEIFSGLHNLQYLYLEYNLIKEISAGTFDSMPNLQLLYLNNNLLKSLPVYIFSGAPLARLNLRNNKFMYLPVSGVLDQLQSLTQIDLEGNPWDCTCDLVALKLWVEKLSDGIVVKELKCETPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPAITFTTPLGPIRSPPGGPVPLSILILSILVVLILTVFVAFCLLVFVLRRNKKPTVKHEGLGNPDCGSMQLQLRKHDHKTNKKDGLSTEAFIPQTIEQMSKSHTCGLKESETGFMFSDPPGQKVTMRNVADKEKDLLHVDTRKRLSTIDELDELFPSRDSNVFIQNFLESKKEYNSIGVSGFEIRYPEKQPDKKSKKSLIGGNHSKIVVEQRKSEYFELKAXLQSSPDYLQVLEEQTALNKINOV62e, CG98011-03SEQ ID NO: 120912469 bpDNA SequenceORF Start: at 7ORF Stop: at 2464GGATCCGATTCTGACATATCGGTGGAAATTTGCAATGTGTGTTCCTGCGTGTCAGTTGAGAATGTGCTCTATGTCAACTGTGAGAAGGTTTCAGTCTACAGGCCAAATCAGCTGAAACCACCTTGGTCTAATTTTTATCACCTCAATTTCCAAAATAATTTTTTAAATATTCTGTATCCAAATACATTCTTGAATTTTTCACATGCAGTCTCCCTGCATCTGGGGAATAATAAACTGCAGAACATTGAGGGAGGAGCCTTTCTTGGGCTCAGTGCATTAAAGCAGTTGCACTTGAACAACAATGAATTAAAGATTCTCCGAGCTGACACTTTCCTTGGCATAGAGAACTTGGAGTATCTCCAGGCTGACTACAATTTAATCMiGTATATTGAACGAGGAGCCTTCAATAAGCTCCACAAACTGAAAGTTCTCATTCTTAATGACAATCTGATTTCATTCCTTCCTGATAATATTTTCCGATTCGCATCTTTGACCCATCTGGATATACGAGGGAACAGAATCCAGAAGCTCCCTTATATCGGGGTTCTGGATCACATTGGCCGTGTCGTTGAATTGCAACTGGAAGATAACCCTTGGAACTGTAGCTGTGATTTATTGCCCTTAAAAGCTTGGCTGGAGAACATGCCATATAACATTTACATAGGAGAAGCTATCTGTGAAACTCCCAGTGACCTATATGGAAGGCTTTTAAAAGAAACCAACAAACAAGAGCTATGTCCCATGGGCACCGGCAGTGATTTTGACGTGCGCATCCTGCCTCCATCTCAGCTGGAAAATGGCTACACCACTCCCAATGGTCACACTACCCAAACATCTTTACACAGATTAGTAACTAAACCACCAAAAACAACAAATCCTTCCAAGATCTCTGGAATCGTTGCAGGCAAAGCCCTCTCCAACCGCAATCTCAGTCAGATTGTGTCTTACCAAACAAGGGTGCCTCCTCTAACACCTTGCCCGGCACCTTGCTTCTGCAAAACACACCCTTCAGATTTGGGACTAAGTGTGAACTGCCAAGAGAAAAATATACAGTCTATGTCTGAACTGATACCGAAACCTTTAAATGCGAAGAAGCTGCACGTCAATGGCAATAGCATCAAGGATGTGGACGTATCAGACTTCACTGACTTTGAAGGACTGGATTTGCTTCATTTAGGCAGCAATCAAATTACAGTGATTAAGGGAGACGTATTTCACAATCTCACTAATTTACGCAGGCTATATCTCAATGGCAATCAAATTGAGAGACTCTATCCTGAAATATTTTCAGGTCTTCATAACCTGCAGTATCCGTATTTGGAATACAATTTGATTAAGGAAATCTCAGCAGGCACCTTTGACTCCATGCCAAATTTGCAGTTACTGTACTTAAACAATAATCTCCTAAAGAGCCTGCCTGTTTACATCTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACAAATTCATGTACCTGCCTGTCAGTGGGGTCCTTGATCAGTTGCAATCTCTTACACAGATTGACTTGGAGGGCAACCCATGGGACTGTACTTGTGACTTGGTGGCATTAAAGCTGTGGGTGGAGAAGTTGAGCGACGGGATTGTTGTGAAAGAACTGAAATGTGAGACGCCTGTTCAGTTTGCCAACATTGAACTGAAGTCCCTCAAAAATGAAATCTTATGTCCCAAACTTTTAAATAAGCCGTCTGCACCATTCACAAGCCCTGCACCTGCCATTACATTCACCACTCCTTTGGGTCCCATTCGAAGTCCTCCTGGTGGGCCAGTGCCTCTGTCTATTTTAATCTTAAGTATCTTAGTGGTCCTCATTTTAACGGTGTTTGTTGCTTTTTGCCTTCTTGTTTTTGTCCTGCGACGCAACAAGAAACCCACAGTGAAGCACGAAGGCCTGGGGAATCCTGACTGTGGCTCCATGCAGCTGCAGCTAAGGAAGCATGACCACAAAACCAATAAAAAAGATGGACTGAGCACAGAAGCTTTCATTCCACAAACTATAGAACAGATGAGCAAGAGCCACACTTGTGGCTTGAAAGAGTCAGAAACTGGGTTCATGTTTTCAGATCCTCCAGGACAGAAAGTTGTTATGAGAAATGTGGCCGACAAGGAGAAAGATTTATTACATGTAGATACCAGGAAGAGACTGAGCACAATTGATGAGCTGGATGAATTATTCCCTAGCAGGGATTCCAATGTGTTTATTCAGAATTTTCTTGAAAGCAAAAAGGAGTATAATAGCATAGGTGTCAGTGGCTTTGAGATCCGCTATCCAGAAAAACAACCAGACAAAAAAAGTAAGAAGTCACTGATAGGTGGCAACCACAGTAAAATTGTTGTGGAACAAAGGAAGAGTGAGTATTTTGAACTGAAGGCGAAACTGCAGAGTTCCCCTGACTACCTACAGGTCCTTGAGGAGCAAACAGCTTTGAACAAGATCCTCGAGNOV62e, CG98011-03Protein SequenceSEQ ID NO: 1210819 aaMW at 92291.4kDDSDISVEICNVCSCVSVENVLYVNCEKVSVYRPNQLKPPWSNFYHLNFQNNFLNILYPNTFLNFSHAVSLHLGNNKLQNIEGGAFLGLSALKQLHLNNNELKILRADTFLGIENLEYLQADYNLIKYIERGAFNKLHKLKVLILNDNLISFLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRVVELQLEDNPWNCSCDLLPLKAWLENMPYNIYIGEAICETPSDLYGRLLKETNKQELCPMGTGSDFDVRILPPSQLENGYTTPNGHTTQTSLHRLVTKPPKTTNPSKISGIVAGKALSNRNLSQIVSYQTRVPPLTPCPAPCFCKTHPSDLGLSVNCQEKNIQSMSELIPKPLNAKKLHVNGNSIKDVDVSDFTDFEGLDLLHLGSNQITVIKGDVFHNLTNLRRLYLNGNQIERLYPEIFSGLHNLQYPYLEYNLIKEISAGTFDSMPNLQLLYLNNNLLKSLPVYIFSGAPLARLNLRNNKFMYLPVSGVLDQLQSLTQIDLEGNPWDCTCDLVALKLWVEKLSDAGIWKELKCETPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPAITFTTPLGPIRSPPGGPVPLSILILSILVVLILTVFVAFCLLVFVLRRNKKPTVKHEGLGNPDCGSMQLQLRKHDHKTNKKDGLSTEAFIPQTIEQMSKSHTCGLKESETGFMFSDPPGQKVVMRNVADKEKDLLHVDTRKRLSTIDELDELFPSRDSNVFIQNFLESKKEYNSIGVSGFEIRYPEKQPDKKSKKSLIGGNHSKIVVEQRKSEYFELKAKLQSSPDYLQVLEEQTALNKINOV62f, CG98011-04SEQ ID NO: 12111800 bpDNA SequenceORF Start: at 7ORF Stop: at 1795GGATCCGATTCTGACATATCGGTGGAAATTTGCAATGTGTGTTCCTGCGTGTCAGTTGAGAATGTGCTCTATGTCAACTGTGAGAAGGTTTCAGTCTACAGACCAAATCAGCTGAAACCACCTTGGTCTAATTTTTATCACCTCAATTTCCAAAATAATTTTTTAAATATTCTGTATCCAAATACATTCTTGAATTTTTCACATGCAGTCTCCCTGCATCTGGGGAATAATAAACTGCAGAACATTGAGGGAGGAGCCTTTCTTGGGCTCAGTGCATTAAAGCAGTTGCACTTGAACAACAATGAATTAAAGATTCTCCGAGCTGACACTTTCCCTGGCATAGAGAACTTGGAGTATCTCCAGGCTGACTACAATTTAATCAAGTATATTGAACGAGGAGCCTTCAATAAGCTCCACAAACTGAAAGTTCTCATTCTTAATGACAATCTGATTTCATTCCTTCCTGATAATATTTTCCGATTCGCATCTTTGACCCATCTGGATATACGAGGGAACAGAATCCAGAAGCTCCCTTATATCGGGGTTTCTGGACACATTGGCCGTGTCGTTGAATTGCAACTGGAAGATAACCCTTGGAACTGTAGCTGTGATTTATTGCCCTTAAAAGCTTGGCTGGAGAACATGCCATATAACATTTACATAGGAGAAGCTATCTGTGAAACTCCCAGTGACTTATATGGAAGGCTTTTAAAAGAAACCAACAAACAAGAGCTATGTCCCATGGGCACCGGCAGTGATTTTGACGTGCGCATCCTGCCTCCATCTCAGCTGGAAAATGGCTACACCACTCCCAATGGTCACACTACCCAAACATCTTTACACAGATTAGTAACTAAACCACCAAAAACAACAAATCCTTCCAAGATCTCTGGAATCGTTGCAGGCAAAGCCCTCTCCAACCGCAATCTCAGTCAGATTGTGTCTTACCAAACAAGGGTGCCTCCTCTAACACCTTGCCCGGCACCTTGCTTCTGCAAAACACACCCTTCAGATTTGGGACTAAGTGTGAACTGCCAAGAGAAAAATATACAGTCTATGTCTGAACTGATACCGAAACCTTTAAATGCGATGAAGCTGCACGTCAATGGCAATAGCATCAAGGATGTGGACGTATCAGACTTCACTGACTTTGAAGGACTGGATTTGCTTCATTTAGGCAGCAATCAAATTACAGTGATTAAGGGAGACGTATTTCACAATCTCACTAATTTACGCAGGCTATATCTCAATGGCAATCAAATTGAGAGACTCTATCCTGAAATATTTTCAGGTCTTCATAACCTGCAGTATCTGTATTTGGAATACAATTTGATTAAGGAAATCTCAGCAGGCACCTTTGACTCCATGCCAAATTTGCAGTTACTGTACTTAAACAATAATCTCCTAAAGAGCCTGCCTGTTTACATCTTTTCCGGAGCACCCTTAGCTAGACTGAACCTGAGGAACAACAAATTCATGTACCTGCCTGTCAGTGGGGTCCTTGATCAGTTGCAATCTCTTACACAGATTGACTTGGAGGGCAACCCATGGGACTGTACTTGTGACTTGGTGGCATTAAAGCTGTGGGTGGGGAAGTTGAGCGACGGGATTGTTGTGAAAGAACTGAAATGTGAGACGCCTGTTCAGTTTGCCAACATTGAACTGAAGTCCCTCAAAAATGAAATCTTATGTCCCAAACTTTTAAATAAGCCGTCTGCACCATTCACAAGCCCTGCACCTACCATTACATTCACCACTCCTTTGGGTCCCATTCGAAGTCCTCCTGGTGGGCCACTCGAGNOV62f, CG98011-04Protein SequenceSEQ ID NO: 1212596 aaMW at 66893.3kDDSDISVEICNVCSCVSVENVLYVNCEKVSVYRPNQLKPPWSNFYHLNFQNNFLNILYPNTFLNFSHAVSLHLGNNKLQNIEGGAFLGLSALKQLHLNNNELKILRADTFPGIENLEYLQADYNLIKYIERGAFNKLHKLKVLILNDNLISFLPDNIFRFASLTHLDIRGNRIQKLPYIGVLEHIGRWELQLEDANPWNCSCDLLPLKWLENMPYNIYIGEAICETPSDLYGRLLKETNKQELCPMGTGSDFDVRILPPSQALENGYTTPNGHTTQTSLHRLVTKPPKTTNPSKISGIVAGKALSNRNLSQIVSYQTRVPPLTPCPAPCFCKTHPSDLGLSVNCQEKNIQSMSELIPKPLNAKKLHVNGNSIKDVDVSDFTDFEGLDLLHLGSNQITVIKGDVFHNLTNLRRLYLNGNQIERLYPEIFSGLHNLQYLYLEYNLIKEISAGTFDSMPNLQLLYLNNNLLKSLPVYIFSGAPLARLNLRNNKFMYLPVSGVLDQLQSLTQIDLEGNPWDCTCDLVALKLWVGKLSDGIVVKELKCETPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPTITFTTPLGPIRSPPGGP


A ClustalW comparison of the above protein sequences yields the following sequence alignment shown in Table 62B.

TABLE 62BComparison of the NOV62 protein sequences.NOV62aMIYIQVIFQVMTIEKMFSFYFLDYFSLFRSIQLFADCKKMFLWLFLILSALISSTNADSDNOV62b-------------------------------------------------------GSDSDNOV62c-------------------------------------------------------GSDSDNOV62dMIYIQVIFQVMTTEKMFSFYFLDYFSLFRSIQLFADCKKMFLWLFLILSALISSTNADSDNOV62e---------------------------------------------------------DSDNOV62f---------------------------------------------------------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-----NOV62cTPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPTITFTTPLGPIRSPPGGPLE-----NOV62dTPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPAITFTTPLGPIRSPPGGPVPLSILINOV62eTPVQFANIELKSLKNEILCPKLLNKPSAPFTSPAPAITFTTPLGPIRSPPGGPVPLSILINOV62fTPVQFANIELKSLKNETLCPKLLNKPSAPFTSPAPTITFTTPLGPIRSPPGGP-------NOV62aLSILVVLILTVFVAFCLLVFVLRRNKKPTVKHEGLGNPDCGSMQLQLRKHDHKTNKKDGLNOV62b------------------------------------------------------------NOV62c------------------------------------------------------------NOV62dLSILVVLILTVFVAFCLLVFVLRRNKKPTVKHEGLGNPDCGSMQLQLRKHDHKTNKKDGLNOV62eLSILVVLILTVFVAFCLLVFVLRRNKKPTVKHEGLGNPDCGSMQLQLRKHDHKTNKKDGLNOV62f------------------------------------------------------------NOV62aSTEAFIPQTIEQMSKSHTCGLKESETGFMFSDPPGQKVVMRNVADKEKDLLHVDTRKRLSNOV62b------------------------------------------------------------NOV62c------------------------------------------------------------NOV62dSTEAFIPQTIEQMSKSHTCGLKESETGFMFSDPPGQKVVMRNVADKEKDLLHVDTRKRLSNOV62eSTEAFIPQTIEQMSKSHTCGLKESETGFMFSDPPGQKVVMRNVADKEKDLLHVDTRKRLSNOV62f------------------------------------------------------------NOV62aTIDELDELFPSRDSNVPIQNFLESKKEYNSIGVSGFETRYPEKQPDKKSKKSLIGGNHSKNOV62b------------------------------------------------------------NOV62c------------------------------------------------------------NOV62dTIDELDELFPSRDSNVFIQNFLESKKEYNSIGVSGFETRYPEKQPDKKSKKSLIGGNHSKNOV62eTIDELDELFPSRDSNVFIQNFLESKKEYNSIGVSGFEIRYPEKQPDKKSKKSLIGGNHSKNOV62f------------------------------------------------------------NOV62aIVVEQRKSEYFELKAKLQSSPDYLQVLEEQTALNKINOV62b------------------------------------NOV62c------------------------------------NOV62dIVVEQRKSEYFELKAKLQSSPDYLQVLEEQTALNKINOV62eIVVEQRKSEYFELKAKLQSSPDYLQVLEEQTALNKINOV62f------------------------------------NOV62a(SEQ ID NO: 1202)NOV62b(SEQ ID NO: 1204)NOV62c(SEQ ID NO: 1206)NOV62d(SEQ ID NO: 1208)NOV62e(SEQ ID NO: 1210)NOV62f(SEQ ID NO: 1212)


Further analysis of the NOV62a protein yielded the following properties shown in Table 62C.

TABLE 62CProtein Sequence Properties NOV62aSignalP analysis:Cleavage site between residues 58 and 59PSORT II analysis:PSG:a new signal peptide prediction methodN-region:length 0; pos. chg 0; neg. chg 0H-region:length 13; peak value 8.16PSG score:3.76GvH:von Heijne's method for signal seq. recognitionGvH score (threshold: −2.1): 2.51possible cleavage site: between 57 and 58>>> Seems to have a cleavable signal peptide (1 to 57)ALOM:Klein et al's method for TM region allocationInit position for calculation: 58Tentative number of TMS(s) for the threshold 0.5: 1Number of TMS(s) for threshold 0.5: 1INTEGRALLikelihood =Transmembrane 663-679−16.61PERIPHERALLikelihood = 2.92 (at 578)ALOM score: −16.61 (number of TMSs: 1)MTOP:Prediction of membrane topology (Hartmann et al.)Center position for calculation: 28Charge difference: 1.0 C(2.0)-N(1.0)C > N: C-terminal side will be inside>>>Caution: Inconsistent mtop result with signal peptide>>> membrane topology: type 1a (cytoplasmic tail 680 to 876)MITDISC:discrimination of mitochondrial targeting seqR content:0Hyd Moment(75):4.59Hyd Moment(95):1.85G content:0D/E content:2S/T content:2Score: −7.15Gavel:prediction of cleavage sites for mitochondrial preseqcleavage site motif not foundNUCDISC: discrimination of nuclear localization signalspat4: nonepat7: PDKKSKK (4) at 825bipartite: nonecontent of basic residues: 10.4%NLS Score: −0.13KDEL:ER retention motif in the C-terminus: noneER Membrane Retention Signals:KKXX-like motif in the C-terminus: ALNKSKL: peroxisomal targeting signal in the C-terminus: NKIPTS2: 2nd peroxisomal targeting signal: noneVAC: possible vacuolar targeting motif: noneRNA-binding motif: noneActinin-type actin-binding motif:type 1: nonetype 2: noneNMYR: N-myristoylation pattern: nonePrenylation motif: nonememYQRL: transport motif from cell surface to Golgi: noneTyrosines in the tail: too long tailDileucine motif in the tail: foundLL at 770checking 63 PROSITE DNA binding motifs: nonechecking 71 PROSITE ribosomal protein motifs: nonechecking 33 PROSITE prokaryotic DNA binding motifs: noneNNCN:Reinhardt's method for Cytoplasmic/Nuclear discriminationPrediction: cytoplasmicReliability: 55.5COIL:Lupas's algorithm to detect coiled-coil regionstotal: 0 residuesFinal Results (k = 9/23):44.4%: endoplasmic reticulum22.2%: Golgi11.1%: plasma membrane11.1%: vesicles of secretory system11.1%: extracellular, including cell wall>> prediction for CG98011-01 is end (k = 9)


A search of the NOV62a protein against the Geneseq database, a proprietary database that contains sequences published in patents and patent publication, yielded several homologous proteins shown in Table 62D.

TABLE 62DGeneseq Results for NOV62aNOV62aIdentities/Residues/Similarities forGeneseqProtein/Organism/LengthMatchthe MatchedExpectIdentifier[Patent #, Date]ResiduesRegionValueAAE18209Human MOL2 protein - Homo sapiens,1 . . . 876876/876 (100%)0.0876 aa. [WO200206339-A2, 24-JAN-2002]1 . . . 876876/876 (100%)AAG67510Amino acid sequence of a human11 . . . 876 866/866 (100%)0.0secreted polypeptide - Homo sapiens,1 . . . 866866/866 (100%)866 aa. [WO200166690-A2, 13-SEP-2001]AAE22221Human toll like receptor like molecule 440 . . . 876 837/837 (100%)0.0(TLR-L4) protein - Homo sapiens, 8371 . . . 837837/837 (100%)aa. [WO200220569-A2, 14-MAR-2002]ABJ01959Human IGFALS similar protein SEQ ID40 . . . 871 832/832 (100%)0.0No 660 - Homo sapiens, 832 aa.1 . . . 832832/832 (100%)[WO200216593-A2, 28-FEB-2002]ABG36257Human peptide encoded by genome-24 . . . 598 575/575 (100%)0.0derived single exon probe SEQ ID1 . . . 575575/575 (100%)25922 - Homo sapiens, 575 aa.[WO200186003-A2, 15-NOV-2001]


In a BLAST search of public sequence databases, the NOV62a protein was found to have homology to the proteins shown in the BLASTP data in Table 62E.

TABLE 62EPublic BLASTP Results for NOV62aNOV62aIdentities/ProteinResidues/Similarities forAccessionMatchthe MatchedExpectNumberProtein/Organism/LengthResiduesPortionValueBAC67207Neuronal transmembrane protein11 . . . 876840/866 (96%)0.0Slitrk4 - Mus musculus (Mouse), 866 1 . . . 866857/866 (97%)aa.Q8IW52Similar to KIAA0848 protein - Homo40 . . . 876 837/837 (100%)0.0sapiens (Human), 837 aa. 1 . . . 837 837/837 (100%)Q8BWB0Hypothetical leucine-rich repeat40 . . . 876814/837 (97%)0.0cysteine-containing subfamily/ 1 . . . 837829/837 (98%)cysteine-rich flanking region - Musmusculus (Mouse), 837 aa.Q8C056Hypothetical leucine-rich repeat40 . . . 876813/837 (97%)0.0cysteine-containing subfamily/ 1 . . . 837828/837 (98%)cysteine-rich flanking region - Musmusculus (Mouse), 837 aa.Q8BYG4Hypothetical leucine-rich repeat40 . . . 821761/782 (97%)0.0cysteine-containing subfamily/ 1 . . . 782775/782 (98%)cysteine-rich flanking region - Musmusculus (Mouse), 782 aa (fragment).


PFam analysis predicts that the NOV62a protein contains the domains shown in the Table 62F.

TABLE 62FDomain Analysis of NOV62aIdentities/Similarities forPfamNOV62athe MatchedDomainMatch RegionRegionExpect ValueLRR218 . . . 239 8/25 (32%)0.2419/25 (76%)LRRCT252 . . . 30218/54 (33%)9.7e−1135/54 (65%)LRRNT380 . . . 41311/36 (31%)0.003924/36 (67%)LRR441 . . . 464 9/25 (36%)0.3116/25 (64%)LRR465 . . . 48813/25 (52%)0.007223/25 (92%)LRR489 . . . 512 6/25 (24%)0.01523/25 (92%)LRR513 . . . 53612/25 (48%)0.0319/25 (76%)LRRCT570 . . . 62014/54 (26%)8.2e−0635/54 (65%)


Example B
Sequencing Methodology and Identification of NOVX Clones

1. GeneCalling™ Technology: This is a proprietary method of performing differential gene expression profiling between two or more samples developed at CuraGen and described by Shimkets, et al., “Gene expression analysis by transcript profiling coupled to a gene database query” Nature Biotechnology 17:198-803 (1999). cDNA was derived from various human samples representing multiple tissue types, normal and diseased states, physiological states, and developmental states from different donors. Samples were obtained as whole tissue, primary cells or tissue cultured primary cells or cell lines. Cells and cell lines may have been treated with biological or chemical agents that regulate gene expression, for example, growth factors, chemokines or steroids. The cDNA thus derived was then digested with up to as many as 120 pairs of restriction enzymes and pairs of linker-adaptors specific for each pair of restriction enzymes were ligated to the appropriate end. The restriction digestion generates a mixture of unique cDNA gene fragments. Limited PCR amplification is performed with primers homologous to the linker adapter sequence where one primer is biotinylated and the other is fluorescently labeled. The doubly labeled material is isolated and the fluorescently labeled single strand is resolved by capillary gel electrophoresis. A computer algorithm compares the electropherograms from an experimental and control group for each of the restriction digestions. This and additional sequence-derived information is used to predict the identity of each differentially expressed gene fragment using a variety of genetic databases. The identity of the gene fragment is confirmed by additional, gene-specific competitive PCR or by isolation and sequencing of the gene fragment.


2. SeqCalling™ Technology: cDNA was derived from various human samples representing multiple tissue types, normal and diseased states, physiological states, and developmental states from different donors. Samples were obtained as whole tissue, primary cells or tissue cultured primary cells or cell lines. Cells and cell lines may have been treated with biological or chemical agents that regulate gene expression, for example, growth factors, chemokines or steroids. The cDNA thus derived was then sequenced using CuraGen's proprietary SeqCalling technology. Sequence traces were evaluated manually and edited for corrections if appropriate. cDNA sequences from all samples were assembled together, sometimes including public human sequences, using bioinformatic programs to produce a consensus sequence for each assembly. Each assembly is included in CuraGen Corporation's database. Sequences were included as components for assembly when the extent of identity with another component was at least 95% over 50 bp. Each assembly represents a gene or portion thereof and includes information on variants, such as splice forms single nucleotide polymorphisms (SNPs), insertions, deletions and other sequence variations.


3. PathCalling™ Technology: The NOVX nucleic acid sequences are derived by laboratory screening of cDNA library by the two-hybrid approach. cDNA fragments covering either the full length of the DNA sequence, or part of the sequence, or both, are sequenced. In silico prediction was based on sequences available in CuraGen Corporation's proprietary sequence databases or in the public human sequence databases, and provided either the full length DNA sequence, or some portion thereof.


The laboratory screening was performed using the methods summarized below:


cDNA libraries were derived from various human samples representing multiple tissue types, normal and diseased states, physiological states, and developmental states from different donors. Samples were obtained as whole tissue, primary cells or tissue cultured primary cells or cell lines. Cells and cell lines may have been treated with biological or chemical agents that regulate gene expression, for example, growth factors, chemokines or steroids. The cDNA thus derived was then directionally cloned into the appropriate two-hybrid vector (Gal4-activation domain (Gal4-AD) fusion). Such cDNA libraries as well as commercially available cDNA libraries from Clontech (Palo Alto, Calif.) were then transferred from E. coli into a CuraGen Corporation proprietary yeast strain (disclosed in U.S. Pat. Nos. 6,057,101 and 6,083,693, incorporated herein by reference in their entireties).


Gal4-binding domain (Gal4-BD) fusions of a CuraGen Corportion proprietary library of human sequences was used to screen multiple Gal4-AD fusion cDNA libraries resulting in the selection of yeast hybrid diploids in each of which the Gal4-AD fusion contains an individual cDNA. Each sample was amplified using the polymerase chain reaction (PCR) using non-specific primers at the cDNA insert boundaries. Such PCR product was sequenced; sequence traces were evaluated manually and edited for corrections if appropriate. cDNA sequences from all samples were assembled together, sometimes including public human sequences, using bioinformatic programs to produce a consensus sequence for each assembly. Each assembly is included in CuraGen Corporation's database. Sequences were included as components for assembly when the extent of identity with another component was at least 95% over 50 bp. Each assembly represents a gene or portion thereof and includes information on variants, such as splice forms single nucleotide polymorphisms (SNPs), insertions, deletions and other sequence variations.


Physical clone: the cDNA fragment derived by the screening procedure, covering the entire open reading frame is, as a recombinant DNA, cloned into pACT2 plasmid (Clontech) used to make the cDNA library. The recombinant plasmid is inserted into the host and selected by the yeast hybrid diploid generated during the screening procedure by the mating of both CuraGen Corporation proprietary yeast strains N106′ and YULH (U.S. Pat. Nos. 6,057,101 and 6,083,693).


4. RACE: Techniques based on the polymerase chain reaction such as rapid amplification of cDNA ends (RACE), were used to isolate or complete the predicted sequence of the cDNA of the invention. Usually multiple clones were sequenced from one or more human samples to derive the sequences for fragments. Various human tissue samples from different donors were used for the RACE reaction. The sequences derived from these procedures were included in the SeqCalling Assembly process described in preceding paragraphs.


5. Exon Linking: The NOVX target sequences identified in the present invention were subjected to the exon linking process to confirm the sequence. PCR primers were designed by starting at the most upstream sequence available, for the forward primer, and at the most downstream sequence available for the reverse primer. In each case, the sequence was examined, walking inward from the respective termini toward the coding sequence, until a suitable sequence that is either unique or highly selective was encountered, or, in the case of the reverse primer, until the stop codon was reached. Such primers were designed based on in silico predictions for the full length cDNA, part (one or more exons) of the DNA or protein sequence of the target sequence, or by translated homology of the predicted exons to closely related human sequences from other species. These primers were then employed in PCR amplification based on the following pool of human cDNAs: adrenal gland, bone marrow, brain—amygdala, brain—cerebellum, brain—hippocampus, brain—substantia nigra, brain—thalamus, brain—whole, fetal brain, fetal kidney, fetal liver, fetal lung, heart, kidney, lymphoma - Raji, mammary gland, pancreas, pituitary gland, placenta, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testis, thyroid, trachea, uterus. Usually the resulting amplicons were gel purified, cloned and sequenced to high redundancy. The PCR product derived from exon linking was cloned into the pCR2.1 vector from Invitrogen. The resulting bacterial clone has an insert covering the entire open reading frame cloned into the pCR2.1 vector. The resulting sequences from all clones were assembled with themselves, with other fragments in CuraGen Corporation's database and with public ESTs. Fragments and ESTs were included as components for an assembly when the extent of their identity with another component of the assembly was at least 95% over 50 bp. In addition, sequence traces were evaluated manually and edited for corrections if appropriate. These procedures provide the sequence reported herein.


6. Physical Clone: Exons were predicted by homology and the intron/exon boundaries were determined using standard genetic rules. Exons were further selected and refined by means of similarity determination using multiple BLAST (for example, tBlastN, BlastX, and BlastN) searches, and, in some instances, GeneScan and Grail. Expressed sequences from both public and proprietary databases were also added when available to further define and complete the gene sequence. The DNA sequence was then manually corrected for apparent inconsistencies thereby obtaining the sequences encoding the full-length protein.


The PCR product derived by exon linking, covering the entire open reading frame, was cloned into the pCR2.1 vector from Invitrogen to provide clones used for expression and screening purposes.


Example C
Quantitative Expression Analysis of Clones in Various Cells and Tissues

The quantitative expression of various clones was assessed using microfiter plates containing RNA samples from a variety of normal and pathology-derived cells, cell lines and tissues using real time quantitative PCR (RTQ PCR). RTQ PCR was performed on an Applied Biosystems ABI PRISM® 7700 or an ABI PRISM® 7900 HT Sequence Detection System. Various collections of samples are assembled on the plates, and referred to as Panel 1 (containing normal tissues and cancer cell lines), Panel 2 (containing samples derived from tissues from normal and cancer sources), Panel 3 (containing cancer cell lines), Panel 4 (containing cells and cell lines from normal tissues and cells related to inflammatory conditions), Panel 5D/5I (containing human tissues and cell lines with an enphasis on metabolic diseases), AI_comprehensive_panel (containing normal tissue and samples from autoinflammatory diseases), Panel CNSD.01 (containing samples from normal and diseased brains) and CNS_neurodegeneration_panel (containing samples from normal and Alzheimer's diseased brains).


RNA integrity from all samples is controlled for quality by visual assessment of agarose gel electropherograms using 28S and 18S ribosomal RNA staining intensity ratio as a guide (2:1 to 2.5:1 28s:18s) and the absence of low molecular weight RNAs that would be indicative of degradation products. Samples are controlled against genomic DNA contamination by RTQ PCR reactions run in the absence of reverse transcriptase using probe and primer sets designed to amplify across the span of a single exon.


First, the RNA samples were normalized to reference nucleic acids such as constitutively expressed genes (for example, □-actin and GAPDH). Normalized RNA (5 ul) was converted to cDNA and analyzed by RTQ-PCR using One Step RT-PCR Master Mix Reagents (Applied Biosystems; Catalog No. 4309169) and gene-specific primers according to the manufacturer's instructions.


In other cases, non-normalized RNA samples were converted to single strand cDNA (sscDNA) using Superscript II (Invitrogen Corporation; Catalog No. 18064-147) and random hexamers according to the manufacturer's instructions. Reactions containing up to 10 □g of total RNA were performed in a volume of 20 □l and incubated for 60 minutes at 42° C. This reaction can be scaled up to 50 □g of total RNA in a final volume of 100 □l. sscDNA samples are then normalized to reference nucleic acids as described previously, using 1×TaqMan® Universal Master mix (Applied Biosystems; catalog No. 4324020), following the manufacturer's instructions.


Probes and primers were designed for each assay according to Applied Biosystems Primer Express Software package (version I for Apple Computer's Macintosh Power PC) or a similar algorithm using the target sequence as input. Default settings were used for reaction conditions and the following parameters were set before selecting primers: primer concentration=250 nM, primer melting temperature (Tm) range=58°-60° C., primer optimal Tm=59° C., maximum primer difference=2° C., probe does not have 5′ G, probe Tm must be 10° C. greater than primer Tm, amplicon size 75 bp to 100 bp. The probes and primers selected (see below) were synthesized by Synthegen (Houston, Tex., USA). Probes were double purified by HPLC to remove uncoupled dye and evaluated by mass spectroscopy to verify coupling of reporter and quencher dyes to the 5′ and 3′ ends of the probe, respectively. Their final concentrations were: forward and reverse primers, 900 nM each, and probe, 200 nM.


PCR conditions: When working with RNA samples, normalized RNA from each tissue and each cell line was spotted in each well of either a 96 well or a 384-well PCR plate (Applied Biosystems). PCR cocktails included either a single gene specific probe and primers set, or two multiplexed probe and primers sets (a set specific for the target clone and another gene-specific set multiplexed with the target probe). PCR reactions were set up using TaqMan® One-Step RT-PCR Master Mix (Applied Biosystems, Catalog No. 4313803) following manufacturer's instructions. Reverse transcription was performed at 48° C. for 30 minutes followed by amplification/PCR cycles as follows: 95° C. 10 min, then 40 cycles of 95° C. for 15 seconds, 60° C. for 1 minute. Results were recorded as CT values (cycle at which a given sample crosses a threshold level of fluorescence) using a log scale, with the difference in RNA concentration between a given sample and the sample with the lowest CT value being represented as 2 to the power of delta CT. The percent relative expression is then obtained by taking the reciprocal of this RNA difference and multiplying by 100. CT values below 28 indicate high expression, CT values between 28 and 32 indicate moderate expression, and CT values between 32 and 35 indicate low expression. CT values above 35 reflect levels of expression that are too low to be reliably measured.


When working with sscDNA samples, normalized sscDNA was used as described previously for RNA samples. PCR reactions containing one or two sets of probe and primers were set up as described previously, using 1×TaqMan® Universal Master mix (Applied Biosystems; catalog No. 4324020), following the manufacturer's instructions. PCR amplification was performed as follows: 95° C. 10 min, then 40 cycles of 95° C. for 15 seconds, 60° C. for 1 minute. Results were analyzed and processed as described previously.


Panels 1, 1.1, 1.2, and 1.3D


The plates for Panels 1, 1.1, 1.2 and 1.3D include 2 control wells (genomic DNA control and chemistry control) and 94 wells containing cDNA from various samples. The samples in these panels are broken into 2 classes: samples derived from cultured cell lines and samples derived from primary normal tissues. The cell lines are derived from cancers of the following types: lung cancer, breast cancer, melanoma, colon cancer, prostate cancer, CNS cancer, squamous cell carcinoma, ovarian cancer, liver cancer, renal cancer, gastric cancer and pancreatic cancer. Cell lines used in these panels are widely available through the American Type Culture Collection (ATCC), a repository for cultured cell lines, and were cultured using the conditions recommended by the ATCC. The normal tissues found on these panels are comprised of samples derived from all major organ systems from single adult individuals or fetuses. These samples are derived from the following organs: adult skeletal muscle, fetal skeletal muscle, adult heart, fetal heart, adult kidney, fetal kidney, adult liver, fetal liver, adult lung, fetal lung, various regions of the brain, the spleen, bone marrow, lymph node, pancreas, salivary gland, pituitary gland, adrenal gland, spinal cord, thymus, stomach, small intestine, colon, bladder, trachea, breast, ovary, uterus, placenta, prostate, testis and adipose.


In the results for Panels 1, 1.1, 1.2 and 1.3D, the following abbreviations are used:

    • ca.=carcinoma,
    • *=established from metastasis,
    • met=metastasis,
    • s cell var=small cell variant,
    • non-s=non-sm=non-small,
    • squam=squamous,
    • pl. elf=pl effusion=pleural effusion,
    • glio=glioma,
    • astro=astrocytoma, and
    • neuro=neuroblastoma.


GENERAL_SCREENING_PANEL_V1.4, V1.5, V1.6 AND 1.7


The plates for Panels 1.4, 1.5, 1.6 and 1.7 include 2 control wells (genomic DNA control and chemistry control) and 88 to 94 wells containing cDNA from various samples. The samples in Panels 1.4, 1.5, 1.6 and 1.7 are broken into 2 classes: samples derived from cultured cell lines and samples derived from primary normal tissues. The cell lines are derived from cancers of the following types: lung cancer, breast cancer, melanoma, colon cancer, prostate cancer, CNS cancer, squamous cell carcinoma, ovarian cancer, liver cancer, renal cancer, gastric cancer and pancreatic cancer. Cell lines used in Panels 1.4, 1.5, 1.6 and 1.7 are widely available through the American Type Culture Collection (ATCC), a repository for cultured cell lines, and were cultured using the conditions recommended by the ATCC. The normal tissues found on Panels 1.4, 1.5, 1.6 and 1.7 are comprised of pools of samples derived from all major organ systems from 2 to 5 different adult individuals or fetuses. These samples are derived from the following organs: adult skeletal muscle, fetal skeletal muscle, adult heart, fetal heart, adult kidney, fetal kidney, adult liver, fetal liver, adult lung, fetal lung, various regions of the brain, the spleen, bone marrow, lymph node, pancreas, salivary gland, pituitary gland, adrenal gland, spinal cord, thymus, stomach, small intestine, colon, bladder, trachea, breast, ovary, uterus, placenta, prostate, testis and adipose. Abbreviations are as described for Panels 1, 1.1, 1.2, and 1.3D.


Panels 2D, 2.2, 2.3, and 2.4


The plates for Panels 2D, 2.2, 2.3 and 2.4 generally include 2 control wells and 94 test samples composed of RNA or cDNA isolated from human tissue procured by surgeons working in close cooperation with the National Cancer Institute's Cooperative Human Tissue Network (CHTN) or the National Disease Research Initiative (NDRI) or from Ardais or Clinomics). The tissues are derived from human malignancies and in cases where indicated many malignant tissues have “matched margins” obtained from noncancerous tissue just adjacent to the tumor. These are termed normal adjacent tissues and are denoted “NAT” in the results below. The tumor tissue and the “matched margins” are evaluated by two independent pathologists (the surgical pathologists and again by a pathologist at NDRI/CHTN/Ardais/Clinomics). Unmatched RNA samples from tissues without malignancy (normal tissues) were also obtained from Ardais or Clinomics. This analysis provides a gross histopathological assessment of tumor differentiation grade. Moreover, most samples include the original surgical pathology report that provides information regarding the clinical stage of the patient. These matched margins are taken from the tissue surrounding (i.e. immediately proximal) to the zone of surgery (designated “NAT”, for normal adjacent tissue, in Table RR). In addition, RNA and cDNA samples were obtained from various human tissues derived from autopsies performed on elderly people or sudden death victims (accidents, etc.). These tissues were ascertained to be free of disease and were purchased from various commercial sources such as Clontech (Palo Alto, Calif.), Research Genetics, and Invitrogen.


HASS Panel V 1.0


The HASS panel v 1.0 plates are comprised of 93 cDNA samples and two controls. Specifically, 81 of these samples are derived from cultured human cancer cell lines that had been subjected to serum starvation, acidosis and anoxia for different time periods as well as controls for these treatments, 3 samples of human primary cells, 9 samples of malignant brain cancer (4 medulloblastomas and 5 glioblastomas) and 2 controls. The human cancer cell lines are obtained from ATCC (American Type Culture Collection) and fall into the following tissue groups: breast cancer, prostate cancer, bladder carcinomas, pancreatic cancers and CNS cancer cell lines. These cancer cells are all cultured under standard recommended conditions. The treatments used (serum starvation, acidosis and anoxia) have been previously published in the scientific literature. The primary human cells were obtained from Clonetics (Walkersville, Md.) and were grown in the media and conditions recommended by Clonetics. The malignant brain cancer samples are obtained as part of a collaboration (Henry Ford Cancer Center) and are evaluated by a pathologist prior to CuraGen receiving the samples. RNA was prepared from these samples using the standard procedures. The genomic and chemistry control wells have been described previously.


ARDAIS Panel V 1.0


The plates for ARDAIS panel v 1.0 generally include 2 control wells and 22 test samples composed of RNA isolated from human tissue procured by surgeons working in close cooperation with Ardais Corporation. The tissues are derived from human lung malignancies (lung adenocarcinoma or lung squamous cell carcinoma) and in cases where indicated many malignant samples have “matched margins” obtained from noncancerous lung tissue just adjacent to the tumor. These matched margins are taken from the tissue surrounding (i.e. immediately proximal) to the zone of surgery (designated “NAT”, for normal adjacent tissue) in the results below. The tumor tissue and the “matched margins” are evaluated by independent pathologists (the surgical pathologists and again by a pathologist at Ardais). Unmatched malignant and non-malignant RNA samples from lungs were also obtained from Ardais. Additional information from Ardais provides a gross histopathological assessment of tumor differentiation grade and stage. Moreover, most samples include the original surgical pathology report that provides information regarding the clinical state of the patient.


ARDAIS Prostate V 1.0


The plates for ARDAIS prostate 1.0 generally include 2 control wells and 68 test samples composed of RNA isolated from human tissue procured by surgeons working in close cooperation with Ardais Corporation. The tissues are derived from human prostate malignancies and in cases where indicated malignant samples have “matched margins” obtained from noncancerous prostate tissue just adjacent to the tumor. These matched margins are taken from the tissue surrounding (i.e. immediately proximal) to the zone of surgery (designated “NAT”, for normal adjacent tissue) in the results below. The tumor tissue and the “matched margins” are evaluated by independent pathologists (the surgical pathologists and again by a pathologist at Ardais). RNA from unmatched malignant and non-malignant prostate samples were also obtained from Ardais. Additional information from Ardais provides a gross histopathological assessment of tumor differentiation grade and stage. Moreover, most samples include the original surgical pathology report that provides information regarding the clinical state of the patient.


ARDAIS Kidney V 1.0


The plates for ARDAIS kidney 1.0 generally include 2 control wells and 44 test samples composed of RNA isolated from human tissue procured by surgeons working in close cooperation with Ardais Corporation. The tissues are derived from human prostate malignancies and in cases where indicated malignant samples have “matched margins” obtained from noncancerous prostate tissue just adjacent to the tumor. These matched margins are taken from the tissue surrounding (i.e. immediately proximal) to the zone of surgery (designated “NAT”, for normal adjacent tissue) in the results below. The tumor tissue and the “matched margins” are evaluated by independent pathologists (the surgical pathologists and again by a pathologist at Ardais). RNA from unmatched malignant and non-malignant prostate samples were also obtained from Ardais. Additional information from Ardais provides a gross histopathological assessment of tumor differentiation grade and stage. Moreover, most samples include the original surgical pathology report that provides information regarding the clinical state of the patient.


Panel 3D and 3.1 and 3.2


The plates of Panel 3D, 3.1, and 3.2 are comprised of 94 cDNA samples and two control samples. Specifically, 92 of these samples are derived from cultured human cancer cell lines, 2 samples of human primary cerebellar tissue and 2 controls. The human cell lines are generally obtained from ATCC (American Type Culture Collection), NCI or the German tumor cell bank and fall into the following tissue groups: Squamous cell carcinoma of the tongue, breast cancer, prostate cancer, melanoma, epidermoid carcinoma, sarcomas, bladder carcinomas, pancreatic cancers, kidney cancers, leukemnias/lymphomas, ovarian/uterine/cervical, gastric, colon, lung and CNS cancer cell lines. In addition, there are two independent samples of cerebellum. These cells are all cultured under standard recommended conditions and RNA extracted using the standard procedures. The cell lines in panel 3D, 3.1, 3.2, 1, 1.1., 1.2, 1.3D, 1.4, 1.5, and 1.6 are of the most common cell lines used in the scientific literature.


Panels 4D, 4R, and 4.1D


Panel 4 includes samples on a 96 well plate (2 control wells, 94 test samples) composed of RNA (Panel 4R) or cDNA (Panels 4D/4.1D) isolated from various human cell lines or tissues related to inflammatory conditions. Total RNA from control normal tissues such as colon and lung (Stratagene, La Jolla, Calif.) and thymus and kidney (Clontech) was employed. Total RNA from liver tissue from cirrhosis patients and kidney from lupus patients was obtained from BioChain (Biochain Institute, Inc., Hayward, Calif.). Intestinal tissue for RNA preparation from patients diagnosed as having Crohn's disease and ulcerative colitis was obtained from the National Disease Research Interchange (NDRI) (Philadelphia, Pa.).


Astrocytes, lung fibroblasts, dermal fibroblasts, coronary artery smooth muscle cells, small airway epithelium, bronchial epithelium, microvascular dermal endothelial cells, microvascular lung endothelial cells, human pulmonary aortic endothelial cells, human umbilical vein endothelial cells were all purchased from Clonetics (Walkersville, Md.) and grown in the media supplied for these cell types by Clonetics. These primary cell types were activated with various cytokines or combinations of cytokines for 6 and/or 12-14 hours, as indicated. The following cytokines were used; IL-1 beta at approximately 1-5 ng/ml, TNF alpha at approximately 5-10 ng/ml, IFN gamma at approximately 20-50 ng/ml, IL-4 at approximately 5-10 ng/ml, IL-9 at approximately 5-10 ng/ml, IL- 13 at approximately 5-10 ng/ml. Endothelial cells were sometimes starved for various times by culture in the basal media from Clonetics with 0.1% serum.


Mononuclear cells were prepared from blood of employees at CuraGen Corporation, using Ficoll. LAK cells were prepared from these cells by culture in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco/Life Technologies, Rockville, Md.), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco) and Interleukin 2 for 4-6 days. Cells were then either activated with 10-20 ng/ml PMA and 1-2 μg/ml ionomycin, IL-12 at 5-10 ng/ml, IFN gamma at 20-50 ng/ml and IL-18 at 5-10 ng/ml for 6 hours. In some cases, mononuclear cells were cultured for 4-5 days in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco) with PHA (phytohemagglutinin) or PWM (pokeweed mitogen) at approximately 5 μg/ml. Samples were taken at 24, 48 and 72 hours for RNA preparation. MLR (mixed lymphocyte reaction) samples were obtained by taking blood from two donors, isolating the mononuclear cells using Ficoll and mixing the isolated mononuclear cells 1:1 at a final concentration of approximately 2×106 cells/mil in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol (5.5×10−5 M) (Gibco), and 10 mM Hepes (Gibco). The MLR was cultured and samples taken at various time points ranging from 1-7 days for RNA preparation.


Monocytes were isolated from mononuclear cells using CD14 Miltenyi Beads, +ve VS selection columns and a Vario Magnet according to the manufacturer's instructions. Monocytes were differentiated into dendritic cells by culture in DMEM 5% fetal calf serum (FCS) (Hyclone, Logan, Utah), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5M (Gibco), and 10 mM Hepes (Gibco), 50 ng/ml GMCSF and 5 ng/ml IL-4 for 5-7 days. Macrophages were prepared by culture of monocytes for 5-7 days in DMEM 5% FCS (Hyclone), 100 μm non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), 10 mM Hepes (Gibco) and 10% AB Human Serum or MCSF at approximately 50 ng/ml. Monocytes, macrophages and dendritic cells were stimulated for 6 and 12-14 hours with lipopolysaccharide (LPS) at 100 ng/ml. Dendritic cells were also stimulated with anti-CD40 monoclonal antibody (Pharmingen) at 10 μg/ml for 6 and 12-14 hours.


CD4 lymphocytes, CD8 lymphocytes and NK cells were also isolated from mononuclear cells using CD4, CD8 and CD56 Miltenyi beads, positive VS selection columns and a Vario Magnet according to the manufacturer's instructions. CD45RA and CD45RO CD4 lymphocytes were isolated by depleting mononuclear cells of CD8, CD56, CD14 and CD19 cells using CD8, CD56, CD14 and CD19 Miltenyi beads and positive selection. CD45RO beads were then used to isolate the CD45RO CD4 lymphocytes with the remaining cells being CD45RA CD4 lymphocytes. CD45RA CD4, CD45RO CD4 and CD8 lymphocytes were placed in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco) and plated at 106 cells/ml onto Falcon 6 well tissue culture plates that had been coated overnight with 0.5 μg/ml anti-CD28 (Pharmingen) and 3 ug/fffl anti-CD3 (OKT3, ATCC) in PBS. After 6 and 24 hours, the cells were harvested for RNA preparation. To prepare chronically activated CD8 lymphocytes, we activated the isolated CD8 lymphocytes for 4 days on anti-CD28 and anti-CD3 coated plates and then harvested the cells and expanded them in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco) and EL-2. The expanded CD8 cells were then activated again with plate bound anti-CD3 and anti-CD28 for 4 days and expanded as before. RNA was isolated 6 and 24 hours after the second activation and after 4 days of the second expansion culture. The isolated NK cells were cultured in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco) and IL-2 for 4-6 days before RNA was prepared.


To obtain B cells, tonsils were procured from NDRI. The tonsil was cut up with sterile dissecting scissors and then passed through a sieve. Tonsil cells were then spun down and resupended at 106 cells/ml in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco). To activate the cells, we used PWM at 5 μg/ml or anti-CD40 (Pharmingen) at approximately 10 μg/ml and IL-4 at 5-10 ng/ml. Cells were harvested for RNA preparation at 24,48 and 72 hours.


To prepare the primary and secondary Th1/Th2 and Tr1 cells, six-well Falcon plates were coated overnight with 10 μg/ml anti-CD28 (Pharmingen) and 2 μg/ml OKT3 (ATCC), and then washed twice with PBS. Umbilical cord blood CD4 lymphocytes (Poietic Systems, German Town, Md.) were cultured at 105-106 cells/ml in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), 10 mM Hepes (Gibco) and IL-2 (4 ng/ml). IL-12 (5 ng/ml) and anti-IL4 (1 □g/ml) were used to direct to Th1, while IL-4 (5 ng/ml) and anti-IFN gamma (1 □g/ml) were used to direct to Th2 and IL-10 at 5 ng/ml was used to direct to Tr1. After 4-5 days, the activated Th1, Th2 and Tr1 lymphocytes were washed once in DMEM and expanded for 4-7 days in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), 10 mM Hepes (Gibco) and IL-2 (1 ng/ml). Following this, the activated Th1, Th2 and Tr1 lymphocytes were re-stimulated for 5 days with anti-CD28/OKT3 and cytokines as described above, but with the addition of anti-CD95L (1 □g/ml) to prevent apoptosis. After 4-5 days, the Th1, Th2 and Tr1 lymphocytes were washed and then expanded again with IL-2 for 4-7 days. Activated Th1 and Tb2 lymphocytes were maintained in this way for a maximum of three cycles. RNA was prepared from primary and secondary Th1, Th2 and Tr1 after 6 and 24 hours following the second and third activations with plate bound anti-CD3 and anti-CD28 mAbs and 4 days into the second and third expansion cultures in Interleukin 2.


The following leukocyte cells lines were obtained from the ATCC: Ramos, EOL-1, KU-812. EOL cells were further differentiated by culture in 0.1 mM dbcAMP at 5×105 cells/ml for 8 days, changing the media every 3 days and adjusting the cell concentration to 5×105 cells/ml. For the culture of these cells, we used DMEM or RPMI (as recommended by the ATCC), with the addition of 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), 10 mM Hepes (Gibco). RNA was either prepared from resting cells or cells activated with PMA at 10 ng/ml and ionomycin at 1 μg/ml for 6 and 14 hours. Keratinocyte line CCD106 and an airway epithelial tumor line NCI-H292 were also obtained from the ATCC. Both were cultured in DMEM 5% FCS (Hyclone), 100 μM non essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco), mercaptoethanol 5.5×10−5 M (Gibco), and 10 mM Hepes (Gibco). CCD1106 cells were activated for 6 and 14 hours with approximately 5 ng/ml TNF alpha and 1 ng/ml IL-1 beta, while NCI-H292 cells were activated for 6 and 14 hours with the following cytokines: 5 ng/ml IL-4, 5 ng/ml IL-9, 5 ng/ml IL-13 and 25 ng/ml IFN gamma.


For these cell lines and blood cells, RNA was prepared by lysing approximately 107 cells/ml using Trizol (Gibco BRL). Briefly, 1/10 volume of bromochloropropane (Molecular Research Corporation) was added to the RNA sample, vortexed and after 10 minutes at room temperature, the tubes were spun at 14,000 rpm in a Sorvall SS34 rotor. The aqueous phase was removed and placed in a 15 ml Falcon Tube. An equal volume of isopropanol was added and left at −20 degrees C. overnight. The precipitated RNA was spun down at 9,000 rpm for 15 min in a Sorvall SS34 rotor and washed in 70% ethanol. The pellet was redissolved in 300 μl of RNAse-free water and 35 μl buffer (Promega) 5 μl DTT, 7 μl RNAsin and 8 μl DNAse were added. The tube was incubated at 37 degrees C. for 30 minutes to remove contaminating genomic DNA, extracted once with phenol chloroform and re-precipitated with 1/10 volume of 3 M sodium acetate and 2 volumes of 100% ethanol. The RNA was spun down and placed in RNAse free water. RNA was stored at −80 degrees C.


AI_comprehensive Panel_v1.0


The plates for AI_comprehensive_panel_v1.0 include two control wells and 89 test samples comprised of cDNA isolated from surgical and postmortem human tissues obtained from the Backus Hospital and Clinomics (Frederick, Md.). Total RNA was extracted from tissue samples from the Backus Hospital in the Facility at CuraGen. Total RNA from other tissues was obtained from Clinomics.


Joint tissues including synovial fluid, synovium, bone and cartilage were obtained from patients undergoing total knee or hip replacement surgery at the Backus Hospital. Tissue samples were immediately snap frozen in liquid nitrogen to ensure that isolated RNA was of optimal quality and not degraded. Additional samples of osteoarthritis and rheumatoid arthritis joint tissues were obtained from Clinomics. Normal control tissues were supplied by Clinomics and were obtained during autopsy of trauma victims.


Surgical specimens of psoriatic tissues and adjacent matched tissues were provided as total RNA by Clinomics. Two male and two female patients were selected between the ages of 25 and 47. None of the patients were taking prescription drugs at the time samples were isolated.


Surgical specimens of diseased colon from patients with ulcerative colitis and Crohns disease and adjacent matched tissues were obtained from Clinomics. Bowel tissue from three female and three male Crohn's patients between the ages of 41-69 were used. Two patients were not on prescription medication while the others were taking dexamethasone, phenobarbital, or tylenol. Ulcerative colitis tissue was from three male and four female patients. Four of the patients were taking lebvid and two were on phenobarbital.


Total RNA from post mortem lung tissue from trauma victims with no disease or with emphysema, asthma or COPD was purchased from Clinomics. Emphysema patients ranged in age from 40-70 and all were smokers, this age range was chosen to focus on patients with cigarette-linked emphysema and to avoid those patients with alpha-1anti-trypsin deficiencies. Asthma patients ranged in age from 36-75, and excluded smokers to prevent those patients that could also have COPD. COPD patients ranged in age from 35-80 and included both smokers and non-smokers. Most patients were taking corticosteroids, and bronchodilators.


In the labels employed to identify tissues in the AI_comprehensive panel_v1.0 panel, the following abbreviations are used:

    • AI=Autoimmunity
    • Syn=Synovial
    • Normal=No apparent disease
    • Rep22/Rep20=individual patients
    • RA=Rheumatoid arthritis
      • Backus=From Backus Hospital
      • OA=Osteoarthritis
    • (SS) (BA) (MF)=Individual patients
    • Adj=Adjacent tissue
    • Match control=adjacent tissues
    • -M=Male
    • -F=Female
    • COPD=Chronic obstructive pulmonary disease


AI.05 Chondrosarcoma


The AI.05 chondrosarcoma plates are comprised of SW1353 cells that had been subjected to serum starvation and treatment with cytokines that are known to induce MMP (1, 3 and 13) synthesis (eg. IL1 beta). These treatments include: IL-1□ (10 ng/ml), IL-1□+ TNF-□ (50 ng/ml), IL-1□+ Oncostatin (50 ng/ml) and PMA(100 ng/ml). The SW1353 cells were obtained from the ATCC (American Type Culture Collection) and were all cultured under standard recommended conditions. The SW1353 cells were plated at 3×105 cells/ml (in DMEM medium-10% FBS) in 6-well plates. The treatment was done in triplicate, for 6 and 18 h. The supernatants were collected for analysis of MMP 1, 3 and 13 production and for RNA extraction. RNA was prepared from these samples using the standard procedures.


Panels 5D and 5I


The plates for Panel 5D and 5I include two control wells and a variety of cDNAs isolated from human tissues and cell lines with an emphasis on metabolic diseases. Metabolic tissues were obtained from patients enrolled in the Gestational Diabetes study. Cells were obtained during different stages in the differentiation of adipocytes from human mesenchymal stem cells. Human pancreatic islets were also obtained.


In the Gestational Diabetes study subjects are young (18-40 years), otherwise healthy women with and without gestational diabetes undergoing routine (elective) Caesarean section. After delivery of the infant, when the surgical incisions were being repaired/closed, the obstetrician removed a small sample (<1 cc) of the exposed metabolic tissues during the closure of each surgical level. The biopsy material was rinsed in sterile saline, blotted and fast frozen within 5 minutes from the time of removal. The tissue was then flash frozen in liquid nitrogen and stored, individually, in sterile screw-top tubes and kept on dry ice for shipment to or to be picked up by CuraGen. The metabolic tissues of interest include uterine wall (smooth muscle), visceral adipose, skeletal muscle (rectus) and subcutaneous adipose. Patient descriptions are as follows:

Patient 2Diabetic Hispanic, overweight, not on insulinPatient 7-9Nondiabetic Caucasian and obese (BMI > 30)Patient 10Diabetic Hispanic, overweight, on insulinPatient 11Nondiabetic African American and overweightPatient 12Diabetic Hispanic on insulin


Adipocyte differentiation was induced in donor progenitor cells obtained from Osirus (a division of Clonetics/BioWhittaker) in triplicate, except for Donor 3U which had only two replicates. Scientists at Clonetics isolated, grew and differentiated human mesenchymal stem cells (HuMSCs) for CuraGen based on the published protocol found in Mark F. Pittenger, et al., Multilineage Potential of Adult Human Mesenchymal Stem Cells Science Apr. 2, 1999: 143-147. Clonetics provided Trizol lysates or frozen pellets suitable for mRNA isolation and ds cDNA production. A general description of each donor is as follows:

Donor 2 and 3UMesenchymalUndifferentiated AdiposeStem cellsDonor 2 and 3AMAdiposeAdipose Midway DifferentiatedDonor 2 and 3ADAdiposeAdipose Differentiated


Human cell lines were generally obtained from ATCC (American Type Culture Collection), NCI or the German tumor cell bank and fall into the following tissue groups: kidney proximal convoluted tubule, uterine smooth muscle cells, small intestine, liver HepG2 cancer cells, heart primary stromal cells, and adrenal cortical adenoma cells. These cells are all cultured under standard recommended conditions and RNA extracted using the standard procedures. All samples were processed at CuraGen to produce single stranded cDNA.


Panel 5I contains all samples previously described with the addition of pancreatic islets from a 58 year old female patient obtained from the Diabetes Research Institute at the University of Miami School of Medicine. Islet tissue was processed to total RNA at an outside source and delivered to CuraGen for addition to panel 5I.


In the labels employed to identify tissues in the 5D and 5I panels, the following abbreviations are used:

    • GO Adipose=Greater Omentum Adipose
    • SK=Skeletal Muscle
    • UT=Uterus
    • PL=Placenta
    • AD=Adipose Differentiated
    • AM=Adipose Midway Differentiated
    • U=Undifferentiated Stem Cells


Human Metabolic RTQ-PCR Panel


The plates for the Human Metabolic RTQ-PCR Panel include two control wells (genomic DNA control and chemistry control) and 211 cDNAs isolated from human tissues and cell lines with an emphasis on metabolic diseases. This panel is useful for establishing the tissue and cellular expression profiles for genes believed to play a role in the etiology and pathogenesis of obesity and/or diabetes and to confirm differential expression of such genes derived from other methods.


Metabolic tissues were obtained from patients enrolled in the CuraGen Gestational Diabetes study and from autopsy tissues from Type II diabetics and age, sex and race-matched control patients. One or more of the following were used to characterize the patients: body mass index [BMI=wt (kg)/ht (m2)], serum glucose, HgbA1c. Cell lines used in this panel are widely available through the American Type Culture Collection (ATCC), a repository for cultured cell lines. RNA from human Pancreatic Islets was also obtained.


In the Gestational Diabetes study, subjects are young (18-40 years), otherwise healthy women with and without gestational diabetes undergoing routine (elective) Caesarian section. After delivery of the infant, when the surgical incisions were being repaired/closed, the obstetrician removed a small sample (<1 cc) of the exposed metabolic tissues during the closure of each surgical level. The biopsy material was rinsed in sterile saline, blotted, and then flash frozen in liquid nitrogen and stored, individually, in sterile screw-top tubes and kept on dry ice for shipment to or to be picked up by CuraGen. The metabolic tissues of interest include uterine wall (smooth muscle), visceral adipose, skeletal muscle (rectus), and subcutaneous adipose. Patient descriptions are as follows:

Patient 7Non-diabetic Caucasian and obesePatient 8Non-diabetic Caucasian and obesePatient 12Diabetic Caucasian with unknown BMI and on insulinPatient 13Diabetic Caucasian, overweight, not on insulinPatient 15Diabetic Caucasian, obese, not on insulinPatient 17Diabetic Caucasian, normal weight, not on insulinPatient 18Diabetic Hispanic, obese, not on insulinPatient 19Non-diabetic Caucasian and normal weightPatient 20Diabetic Caucasian, overweight, and on insulinPatient 21Non-diabetic Caucasian and overweightPatient 22Diabetic Caucasian, normal weight, on insulinPatient 23Non-diabetic Caucasian and overweightPatient 25Diabetic Caucasian, normal weight, not on insulinPatient 26Diabetic Caucasian, obese, on insulinPatient 27Diabetic Caucasian, obese, on insulin


Total RNA was isolated from metabolic tissues of 12 Type II diabetic patients and 12 matched control patients included hypothalamus, liver, pancreas, small intestine, psoas muscle, diaphragm muscle, visceral adipose, and subcutaneous adipose. The diabetics and non-diabetics were matched for age, sex, ethnicity, and BMI where possible.


The panel also contains pancreatic islets from a 22 year old male patient (with a BMI of 35) obtained from the Diabetes Research Institute at the University of Miami School of Medicine. Islet tissue was processed to total RNA at CuraGen.


Cell lines used in this panel are widely available through the American Type Culture Collection (ATCC), a repository for cultured cell lines, and were cultured at an outside facility. The RNA was extracted at CuraGen according to CuraGen protocols. All samples were then processed at CuraGen to produce single stranded cDNA.


In the labels used to identify tissues in the Human Metabolic panel, the following abbreviations are used:

Panel CNSD.01Pl =placentaGo =greater omentumSk =skeletal muscleUt =uterusCC =CaucasianHI =HispanicAA =African AmericanAS =AsianDiab =Type II diabeticNorm =Non-diabeticOverwt =Overweight; med BMIObese =Hi BMILow BM =20-25Med BM =26-30Hi BMI =Greater than 30M =Male# =Patient identifierVis. =VisceralSubQ =Subcutaneous


The plates for Panel CNSD.01 include two control wells and 94 test samples comprised of cDNA isolated from postmortem human brain tissue obtained from the Harvard Brain Tissue Resource Center. Brains are removed from calvaria of donors between 4 and 24 hours after death, sectioned by neuroanatomists, and frozen at −80° C. in liquid nitrogen vapor. All brains are sectioned and examined by neuropathologists to confirm diagnoses with clear associated neuropathology.


Disease diagnoses are taken from patient records. The panel contains two brains from each of the following diagnoses: Alzheimer's disease, Parkinson's disease, Huntington's disease, Progressive Supernuclear Palsy, Depression, and “Normal controls”. Within each of these brains, the following regions are represented: cingulate gyrus, temporal pole, globus palladus, substantia nigra, Brodman Area 4 (primary motor strip), Brodman Area 7 (parietal cortex), Brodman Area 9 (prefrontal cortex), and Brodman area 17 (occipital cortex). Not all brain regions are represented in all cases; e.g., Huntington's disease is characterized in part by neurodegeneration in the globus palladus, thus this region is impossible to obtain from confirmed Huntington's cases. Likewise Parkinson's disease is characterized by degeneration of the substantia nigra making this region more difficult to obtain. Normal control brains were examined for neuropathology and found to be free of any pathology consistent with neurodegeneration.


In the labels employed to identify tissues in the CNS panel, the following abbreviations are used:

    • PSP=Progressive supranuclear palsy
    • Sub Nigra=Substantia nigra
    • Glob Palladus=Globus palladus
    • Temp Pole=Temporal pole
    • Cing Gyr=Cingulate gyrus
    • BA 4=Brodman Area 4


Panel CNS_Neurodegeneration_V1.0


The plates for Panel CNS_Neurodegeneration_V1.0 include two control wells and 47 test samples comprised of cDNA isolated from postmortem human brain tissue obtained from the Harvard Brain Tissue Resource Center (McLean Hospital) and the Human Brain and Spinal Fluid Resource Center (VA Greater Los Angeles Healthcare System). Brains are removed from calvaria of donors between 4 and 24 hours after death, sectioned by neuroanatomists, and frozen at −80° C. in liquid nitrogen vapor. All brains are sectioned and examined by neuropathologists to confirm diagnoses with clear associated neuropathology.


Disease diagnoses are taken from patient records. The panel contains six brains from Alzheimer's disease (AD) patients, and eight brains from “Normal controls” who showed no evidence of dementia prior to death. The eight normal control brains are divided into two categories: Controls with no dementia and no Alzheimer's like pathology (Controls) and controls with no dementia but evidence of severe Alzheimer's like pathology, (specifically senile plaque load rated as level 3 on a scale of 0-3; 0=no evidence of plaques, 3=severe AD senile plaque load). Within each of these brains, the following regions are represented: hippocampus, temporal cortex (Brodman Area 21), parietal cortex (Brodman area 7), and occipital cortex (Brodman area 17). These regions were chosen to encompass all levels of neurodegeneration in AD. The hippocampus is a region of early and severe neuronal loss in AD; the temporal cortex is known to show neurodegeneration in AD after the hippocampus; the parietal cortex shows moderate neuronal death in the late stages of the disease; the occipital cortex is spared in AD and therefore acts as a “control” region within AD patients. Not all brain regions are represented in all cases.


In the labels employed to identify tissues in the CNS_Neurodegeneration_V1.0 panel, the following abbreviations are used:

    • AD=Alzheimer's disease brain; patient was demented and showed AD-like pathology upon autopsy
    • Control=Control brains; patient not demented, showing no neuropathology
    • Control (Path)=Control brains; pateint not demented but showing sever AD-like pathology
    • SupTemporal Ctx=Superior Temporal Cortex
    • Inf Temporal Ctx=Inferior Temporal Cortex


The expression of the gene was analyzed after normalization using scaling factor. The scaling factor is calculated from the Grand mean of CT values for a panel and the Well mean which is specific to the tissue. The Grand mean is the average CT value for all wells across all runs. For example, if a panel has 50 samples and has had 100 probe/primer sets run on it, the grand mean would be the average of these 5000 CT scores. The well mean is tissue-specific. On the above described panel there would be 50 different well means, each taking the average of the 100 CT values generated for each sample on the panel from the 100 probe/primer sets.


The asumption is that across a large number of genes, all samples should have the same CT value. If a well is lower than the average across a large number of genes, it is “scaled up” by that difference or the “scaling factor”.


Scaling Factor=Grand mean−Well mean


The new CT value for the well is:


Scaled CT value=Raw CT+Scaling Factor.


Statistical Analysis of CNS_Neurodegeneration_V1.0 Data


All data were analyzed by analysis of covariance (ANCOVA). As a covariate, the average CT value (or number of rounds of PCR until signal from the well was detected) was calculated for 1000 PCR runs on different genes. This number is therefore an estimate of total cDNA quantity and quality for each sample. When RTQ PCR is run for a given gene, CT values are therefore compared to these average values to correct for differences in well loading or original RNA quality. Stats were run on data from the temporal cortex, as this regions shows sever neurodegeneration in the mid to late stages of the disease, and because the largest number of samples were available for this region giving the most statistical power. Covariates for each well corresponding to Temporal Cortex samples are listed below. The well numbers (10-25) are listed under “Order” in the table of CT values given for each gene run. For this analysis, Controls and Control (Path) cases were grouped together as the intention was to find genes associated with dementia as opposed to amyloid deposition.

Panel CNS_Neurodegeneration_V2.010AD133.01411AD232.30912AD334.19513AD432.68914AD5 Inf30.82915AD5 Sup31.51916AD6 Inf31.51717AD6 Sup31.41518Con134.23619Con232.35220Con333.21521Con433.66122Con5 (Path)31.68523Con6 (Path)32.18724Con7 (Path)34.42725Con8 (Path)32.238


The plates for Panel CNS_Neurodegeneration_V2.0 include two control wells and 47 test samples comprised of cDNA isolated from postmortem human brain tissue obtained from the Harvard Brain Tissue Resource Center (McLean Hospital) and the Human Brain and Spinal Fluid Resource Center (VA Greater Los Angeles Healthcare System). Brains are removed from calvaria of donors between 4 and 24 hours after death, sectioned by neuroanatomists, and frozen at −80° C. in liquid nitrogen vapor. All brains are sectioned and examined by neuropathologists to confirm diagnoses with clear associated neuropathology.


Disease diagnoses are taken from patient records. The panel contains sixteen brains from Alzheimer's disease (AD) patients, and twenty-nine brains from “Normal controls” who showed no evidence of dementia prior to death. The twenty-nine normal control brains are divided into two categories: Fourteen controls with no dementia and no Alzheimer's like pathology (Controls) and fifteen controls with no dementia but evidence of severe Alzheimer's like pathology, (specifically senile plaque load rated as level 3 on a scale of 0-3; 0=no evidence of plaques, 3=severe AD senile plaque load). Tissue from the temporal cotex (Broddmann Area 21) was selected for all samples from the Harvard Brain Tissue Resource Center; from the two sample from the Human Brain and Spinal Fluid Resource Center (samples 1 and 2) tissue from the inferior and superior temporal cortex was used; each sample on the panel represents a pool of inferior and superior temporal cortex from an individual patient. The temporal cortex was chosen as it shows a loss of neurons in the intermediate stages of the disease. Selection of a region which is affected in the early stages of Alzheimer's disease (e.g., hippocampus or entorhinal cortex) could potentially result in the examination of gene expression after vulnerable neurons are lost, and missing genes involved in the actual neurodegeneration process.


In the labels employed to identify tissues in the CNS_Neurodegeneration_V2.0 panel, the following abbreviations are used:

    • AD=Alzheimer's disease brain; patient was demented and showed AD-like pathology upon autopsy
    • Control=Control brains; patient not demented, showing no neuropathology
      • AH3=Control brains; pateint not demented but showing sever AD-like pathology
    • Inf & Sup Temp Ctx Pool=Pool of inferior and superior temporal cortex for a given individual


A. CG50253-01: Epidermal Growth Factor


Expression of full-length physical clone CG50253-01 was assessed using the primer-probe set Ag2505, described in Table AA. Results of the RTQ-PCR runs are shown in Tables AB, AC, AD, AE, AF and AG.

TABLE AAProbe Name Ag2505StartSEQ IDPrimersSequencesLengthPosition NoForward5′-aaagaaggataccagggtgatg-3′229691213ProbeTET-5′-atgattgaaccttcaggtccaattca-3′2610201214Reverse5′-ggtaccatttccctttggtaca-3′2210461215









TABLE AB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
15.3



110980 COPD-F
11.8



110968 COPD-M
8.9



110977 COPD-M
28.1



110989 Emphysema-F
9.6



110992 Emphysema-F
1.9



110993 Emphysema-F
7.7



110994 Emphysema-F
5.2



110995 Emphysema-F
3.6



110996 Emphysema-F
0.4



110997 Asthma-M
4.6



111001 Asthma-F
2.3



111002 Asthma-F
3.5



111003 Atopic Asthma-F
22.5



111004 Atopic Asthma-F
10.4



111005 Atopic Asthma-F
7.3



111006 Atopic Asthma-F
1.6



111417 Allergy-M
5.1



112347 Allergy-M
1.4



112349 Normal Lung-F
0.7



112357 Normal Lung-F
7.1



112354 Normal Lung-M
7.6



112374 Crohns-F
9.0



112389 Match Control Crohns-F
11.2



112375 Crohns-F
10.2



112732 Match Control Crohns-F
1.2



112725 Crohns-M
0.9



112387 Match Control Crohns-M
11.4



112378 Crohns-M
1.3



112390 Match Control Crohns-M
16.7



112726 Crohns-M
21.8



112731 Match Control Crohns-M
15.3



112380 Ulcer Col-F
5.8



112734 Match Control Ulcer Col-F
3.7



112384 Ulcer Col-F
19.2



112737 Match Control Ulcer Col-F
13.5



112386 Ulcer Col-F
8.4



112738 Match Control Ulcer Col-F
3.8



112381 Ulcer Col-M
5.0



112735 Match Control Ulcer Col-M
9.7



112382 Ulcer Col-M
11.7



112394 Match Control Ulcer Col-M
3.1



112383 Ulcer Col-M
5.1



112736 Match Control Ulcer Col-M
5.0



112423 Psoriasis-F
14.0



112427 Match Control Psoriasis-F
16.7



112418 Psoriasis-M
14.0



112723 Match Control Psoriasis-M
0.2



112419 Psoriasis-M
18.2



112424 Match Control Psoriasis-M
6.8



112420 Psoriasis-M
13.9



112425 Match Control Psoriasis-M
13.6



104689 (MF) OA Bone-Backus
25.3



104690 (MF) Adj “Normal” Bone-Backus
27.9



104691 (MF) OA Synovium-Backus
2.9



104692 (BA) OA Cartilage-Backus
0.0



104694 (BA) OA Bone-Backus
5.8



104695 (BA) Adj “Normal” Bone-Backus
14.1



104696 (BA) OA Synovium-Backus
2.3



104700 (SS) OA Bone-Backus
28.9



104701 (SS) Adj “Normal” Bone-Backus
25.5



104702 (SS) OA Synovium-Backus
11.7



117093 OA Cartilage Rep7
7.5



112672 OA Bone5
19.2



112673 OA Synovium5
6.4



112674 OA Synovial Fluid cells5
6.8



117100 OA Cartilage Rep14
1.9



112756 OA Bone9
26.4



112757 OA Synovium9
2.8



112758 OA Synovial Fluid Cells9
8.1



117125 RA Cartilage Rep2
14.8



113492 Bone2 RA
84.7



113493 Synovium2 RA
40.9



113494 Syn Fluid Cells RA
61.1



113499 Cartilage4 RA
90.1



113500 Bone4 RA
100.0



113501 Synovium4 RA
71.2



113502 Syn Fluid Cells4 RA
48.6



113495 Cartilage3 RA
77.9



113496 Bone3 RA
92.0



113497 Synovium3 RA
53.6



113498 Syn Fluid Cells3 RA
98.6



117106 Normal Cartilage Rep20
3.0



113663 Bone3 Normal
2.0



113664 Synovium3 Normal
0.5



113665 Syn Fluid Cells3 Normal
1.6



117107 Normal Cartilage Rep22
3.7



113667 Bone4 Normal
4.3



113668 Synovium4 Normal
9.1



113669 Syn Fluid Cells4 Normal
6.6









Column A - Rel. Exp. (%) Ag2505, Run 248588456














TABLE AC










CNS_neurodegeneration_v1.0











Tissue Name
A
B















AD 1 Hippo
38.7
51.4



AD 2 Hippo
48.0
64.6



AD 3 Hippo
18.8
30.6



AD 4 Hippo
34.6
33.0



AD 5 Hippo
36.9
39.2



AD 6 Hippo
100.0
97.9



Control 2 Hippo
26.8
33.7



Control 4 Hippo
39.8
52.5



Control (Path) 3 Hippo
18.4
21.8



AD 1 Temporal Ctx
48.6
58.6



AD 2 Temporal Ctx
45.1
43.2



AD 3 Temporal Ctx
37.4
33.4



AD 4 Temporal Ctx
50.3
66.4



AD 5 Inf Temporal Ctx
23.0
26.4



AD 5 Sup Temporal Ctx
42.3
43.8



AD 6 Inf Temporal Ctx
88.3
100.0



AD 6 Sup Temporal Ctx
70.2
88.3



Control 1 Temporal Ctx
16.6
16.3



Control 2 Temporal Ctx
16.7
21.6



Control 3 Temporal Ctx
7.2
12.9



Control 3 Temporal Ctx
19.8
43.5



Control (Path) 1 Temporal Ctx
20.6
24.1



Control (Path) 2 Temporal Ctx
20.6
32.1



Control (Path) 3 Temporal Ctx
14.0
17.8



Control (Path) 4 Temporal Ctx
20.9
23.3



AD 1 Occipital Ctx
31.9
35.4



AD 2 Occipital Ctx (Missing)
5.2
4.8



AD 3 Occipital Ctx
16.7
23.5



AD 4 Occipital Ctx
39.5
46.3



AD 5 Occipital Ctx
24.7
22.8



AD 6 Occipital Ctx
59.9
87.7



Control 1 Occipital Ctx
19.8
14.9



Control 2 Occipital Ctx
26.2
29.9



Control 3 Occipital Ctx
15.2
15.7



Control 4 Occipital Ctx
34.2
36.6



Control (Path) 1 Occipital Ctx
25.5
24.1



Control (Path) 2 Occipital Ctx
15.1
20.3



Control (Path) 3 Occipital Ctx
9.5
14.2



Control (Path) 4 Occipital Ctx
30.6
37.6



Control 1 Parietal Ctx
13.3
14.0



Control 2 Parietal Ctx
46.3
57.8



Control 3 Parietal Ctx
10.1
12.9



Control (Path) 1 Parietal Ctx
24.5
29.5



Control (Path) 2 Parietal Ctx
28.3
35.6



Control (Path) 3 Parietal Ctx
12.7
18.9



Control (Path) 4 Parietal Ctx
28.3
32.5









Column A - Rel. Exp. (%) Ag2505, Run 208123723






Column B - Rel. Exp. (%) Ag2505, Run 224116291














TABLE AD










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
1.8



Pancreas
13.7



Pancreatic ca. CAPAN 2
1.5



Adrenal gland
3.6



Thyroid
100.0



Salivary gland
4.1



Pituitary gland
37.6



Brain (fetal)
44.1



Brain (whole)
9.3



Brain (amygdala)
8.1



Brain (cerebellum)
1.8



Brain (hippocampus)
10.2



Brain (substantia nigra)
29.3



Brain (thalamus)
3.6



Cerebral Cortex
7.7



Spinal cord
15.2



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.3



neuro*; met SK-N-AS
0.4



astrocytoma SF-539
1.8



astrocytoma SNB-75
2.7



glioma SNB-19
0.0



glioma U251
9.3



glioma SF-295
0.4



Heart (fetal)
10.0



Heart
3.1



Skeletal muscle (fetal)
12.8



Skeletal muscle
20.9



Bone marrow
1.2



Thymus
6.0



Spleen
6.7



Lymph node
6.7



Colorectal
23.5



Stomach
12.0



Small intestine
54.3



Colon ca. SW480
1.1



Colon ca.* SW620(SW480 met)
1.4



Colon ca. HT29
7.3



Colon ca. HCT-116
7.3



Colon ca. CaCo-2
10.7



Colon ca. tissue(ODO3866)
8.5



Colon ca. HCC-2998
2.9



Gastric ca.* (liver met) NCI-N87
71.7



Bladder
14.8



Trachea
21.9



Kidney
38.2



Kidney (fetal)
27.5



Renal ca. 786-0
0.0



Renal ca. A498
0.2



Renal ca. RXF 393
39.8



Renal ca. ACHN
51.1



Renal ca. UO-31
0.2



Renal ca. TK-10
0.0



Liver
1.4



Liver (fetal)
2.3



Liver ca. (hepatoblast) HepG2
11.8



Lung
75.3



Lung (fetal)
54.7



Lung ca. (small cell) LX-1
5.5



Lung ca. (small cell) NCI-H69
5.6



Lung ca. (s. cell var.) SHP-77
0.2



Lung ca. (large cell)NCI-H460
1.2



Lung ca. (non-sm. cell) A549
1.2



Lung ca. (non-s. cell) NCI-H23
3.2



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
1.8



Lung ca. (squam.) NCI-H596
14.6



Mammary gland
11.9



Breast ca.* (pl. ef) MCF-7
89.5



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
24.7



Breast ca. BT-549
2.3



Breast ca. MDA-N
0.0



Ovary
3.5



Ovarian ca. OVCAR-3
6.4



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
0.2



Ovarian ca. IGROV-1
14.5



Ovarian ca.* (ascites) SK-OV-3
9.3



Uterus
27.7



Placenta
2.9



Prostate
25.0



Prostate ca.* (bone met)PC-3
0.0



Testis
2.5



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
19.3









Column A - Rel. Exp. (%) Ag2505, Run 165531061














TABLE AE










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.2



Secondary Th1 rest
0.0



Secondary Th2 rest
0.3



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.2



Primary Tr1 act
0.1



Primary Th1 rest
0.1



Primary Th2 rest
0.0



Primary Tr1 rest
0.1



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.1



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.2



B lymphocytes CD40L and IL-4
0.1



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.1



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.3



HUVEC IL-1beta
0.2



HUVEC IFN gamma
0.1



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.1



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1 beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.2



Bronchial epithelium TNFalpha + IL1beta
2.0



Small airway epithelium none
0.5



Small airway epithelium TNFalpha + IL-1beta
19.6



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
2.0



Astrocytes TNFalpha + IL-1beta
2.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.4



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
1.3



Liver cirrhosis
7.5



Lupus kidney
13.3



NCI-H292 none
21.8



NCI-H292 IL-4
42.0



NCI-H292 IL-9
41.8



NCI-H292 IL-13
20.9



NCI-H292 IFN gamma
14.4



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.5



Lung fibroblast none
4.5



Lung fibroblast TNF alpha + IL-1 beta
0.3



Lung fibroblast IL-4
14.6



Lung fibroblast IL-9
3.9



Lung fibroblast IL-13
8.7



Lung fibroblast IFN gamma
14.9



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.2



IBD Colitis 2
0.3



IBD Crohn's
9.9



Colon
41.2



Lung
61.6



Thymus
100.0



Kidney
21.3









Column A - Rel. Exp. (%) Ag2505, Run 164318134














TABLE AF










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
32.3



97476_Patient-07sk_skeletal muscle
8.2



97477_Patient-07ut_uterus
31.4



97478_Patient-07pl_placenta
3.5



99167_Bayer Patient 1
9.5



97482_Patient-08ut_uterus
90.1



97483_Patient-08pl_placenta
7.4



97486_Patient-09sk_skeletal muscle
1.4



97487_Patient-09ut_uterus
78.5



97488_Patient-09pl_placenta
0.6



97492_Patient-10ut_uterus
66.0



97493_Patient-10pl_placenta
3.1



97495_Patient-11go_adipose
28.3



97496_Patient-11sk_skeletal muscle
5.8



97497_Patient-11ut_uterus
35.4



97498_Patient-11pl_placenta
2.0



97500_Patient-12go_adipose
35.1



97501_Patient-12sk_skeletal muscle
9.9



97502_Patient-12ut_uterus
100.0



97503_Patient-12pl_placenta
4.1



94721_Donor 2 U - A_Mesenchymal Stem Cells
0.0



94722_Donor 2 U - B_Mesenchymal Stem Cells
0.0



94723_Donor 2 U - C_Mesenchymal Stem Cells
0.0



94709_Donor 2 AM - A_adipose
0.0



94710_Donor 2 AM - B_adipose
0.0



94711_Donor 2 AM - C_adipose
0.0



94712_Donor 2 AD - A_adipose
0.0



94713_Donor 2 AD - B_adipose
0.0



94714_Donor 2 AD - C_adipose
0.0



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
0.0



94730_Donor 3 AM - A_adipose
0.0



94731_Donor 3 AM - B_adipose
0.0



94732_Donor 3 AM - C_adipose
0.2



94733_Donor 3 AD - A_adipose
0.0



94734_Donor 3 AD - B_adipose
0.0



94735_Donor 3 AD - C_adipose
0.0



77138_Liver_HepG2untreated
21.2



73556_Heart_Cardiac stromal cells (primary)
0.0



81735_Small Intestine
36.9



72409_Kidney_Proximal Convoluted Tubule
4.6



82685_Small intestine_Duodenum
27.0



90650_Adrenal_Adrenocortical adenoma
0.3



72410_Kidney_HRCE
7.2



72411_Kidney_HRE
10.7



73139_Uterus_Uterine smooth muscle cells
0.0









Column A - Rel. Exp. (%) Ag2505, Run 248045752














TABLE AG










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
7.7



Colon NAT 1
2.5



Colon cancer 2
26.4



Colon NAT 2
8.4



Colon cancer 3
38.2



Colon NAT 3
16.7



Colon malignant cancer 4
69.7



Colon NAT 4
8.7



Lung cancer 1
7.2



Lung NAT 1
8.0



Lung cancer 2
26.1



Lung NAT 2
17.4



Squamous cell carcinoma 3
19.6



Lung NAT 3
8.3



Metastatic melanoma 1
14.5



Melanoma 2
1.1



Melanoma 3
2.5



Metastatic melanoma 4
13.5



Metastatic melanoma 5
12.6



Bladder cancer 1
1.0



Bladder NAT 1
0.0



Bladder cancer 2
2.4



Bladder NAT 2
0.3



Bladder NAT 3
0.7



Bladder NAT 4
1.5



Prostate adenocarcinoma 1
100.0



Prostate adenocarcinoma 2
12.9



Prostate adenocarcinoma 3
15.1



Prostate adenocarcinoma 4
15.9



Prostate NAT 5
14.7



Prostate adenocarcinoma 6
13.1



Prostate adenocarcinoma 7
16.2



Prostate adenocarcinoma 8
4.5



Prostate adenocarcinoma 9
33.4



Prostate NAT 10
7.7



Kidney cancer 1
9.6



Kidney NAT 1
33.4



Kidney cancer 2
19.8



Kidney NAT 2
64.6



Kidney cancer 3
7.1



Kidney NAT 3
29.7



Kidney cancer 4
8.5



Kidney NAT 4
7.9









Column A - Rel. Exp. (%) Ag2505, Run 267145080







AI_comprehensive panel_v1.0 Summary: Ag2505 Highest expression of this gene was seen in a sample derived from rheumatoid arthritis bone (CT=27). This gene displayed ubiquitous expression, but expression of this gene was higher in bone, synovium, cartilage and synovial fluid from RA patients as compared to expression in samples from OA patients, normal and diseased lung samples. Expression of this gene was downregulated in Crohn's samples as compared to the corresponding control samples. This gene encodes a putative novel adhesion molecule which is homologous to mouse POEM (preosteoblast epidermal growth factor-like repeat protein with meprin)or nephronectin. Murine nephronectin may function in multiple biological processes including development of the kidney (Miner J H. J Cell Biol 2001 Jul 23; 154(2):257-9) and bone and contribute to liver and lung fibrosis (Levine et al., 2000, Am J Pathol 2000 June; 156(6):1927-35). Therapeutic modulation of this gene or gene product is useful in the treatment of autoimmune and inflammatory diseases such as rheumatoid and osteoarthritis, Inflammatory bowel disease, COPD, asthma, psoriasis, liver and lung fibrosis.


CNS_neurodegeneration_v1.0 Summary: Ag2505 Greater expression of this gene in the temporal cortex of Alzheimer's disease patients demonstrates a functional role for this gene, an EGF homolog, in neurodegenerative disease. Alpha secretase activity, which is generally believed to be a beneficial processing alternative to beta secretase, is increased by EGF in neuronal cells. (Slack B E. C.Biochem J 1997 October 1;327 ( Pt 1):245-9) This suggests the increased expression observed here is a compensatory action in the brain to counter the mechanisms of Alzheimer's Disease. This gene or the protein encoded by this gene is a potential therapeutic agent for the treatment of Alzheimer's disease and other neurodegenerative diseases.


EGF is also known to facilitate long term potentiation (LTP) in the hippocampus, a process thought to underlie learning and memory. (Abe K Brain Res 1992 Octobter 16;593(2):335-8 ) This gene has utility in treating disorders of memory, such as neurodegenerative diseases and aging, when used alone or incombination with other growth factors such as bFGF.


In addition, EGF supports the growth and differentiation of dopaniinergic neurons, which are selectively vulnerable to loss in Parkinson's disease. (Storch A. Exp Neurol 2001 August; 170(2):317-25 ). This gene or gene product has utility in treating Parkinson's Disease.


Panel 1.3D Summary: Ag2505 Highest expression of this gene was detected in the thyroid (CT=29.3), with lower but still significant levels of expression seen in other metabolic tissues, including skeletal muscle, fetal skeletal muscle, small intestine, stomach, pancreas, adipose and fetal heart. Very low levels were also seen in heart and placenta. This gene encodes a putative novel adhesion molecule. Studies in mouse have revealed a gene (perhaps the mouse ortholog of this human gene) very homologous to this gene that is called POEM (preosteoblast epidermal growth factor-like repeat protein with meprin, A5 protein, and receptor protein-tyrosine phosphatase mu domain; Morimura N et al., J Biol Chem 2001 November 9;276(45):42172-81) or nephronectin (Brandenberger R et al., J Cell Biol 2001 July 23;154(2):447-58). POEM/nephronectin seems to be a ligand for the alpha8betal integrin as evidenced by two independent sets of published data. Integrins are known to mediate development and organogenesis. (Schwartz et al., Annu. Rev. Cell Dev. Biol. 11, 549-599, 1995; Clark and Brugge, Science 268, 233-239, 1995)Other known ligands for the alpha8beta1 integrin include fibronectin, vitronectin, tenascin, and osteopontin. Modulation of the expression or activity of this gene or gene product by protein, small molecule, or antibody therapeutics is an effective therapeutic for disorders involving alpha8beta1 integrin signaling.


Overall, this gene is expressed at a low to moderate level in the normal tissues on this panel. The brain, prostate, lung and colon cancer cell lines show a very low level of expression compared to the normal organs. This molecule is a candidate as a therapeutic inhibitor for these cancers.


Expression in the brain in the substantia nigra, hippocampus, cortex, amygdala, thalamus and spinal cord indicates a further functional role for this gene product in CNS processes mediated by these regions. Please see CNS_neurodegeneration_v1.0 for discussion of utility in the central nervous system.


Panel 4D Summary: Ag 2505 Highest expression of this transcript was found in the thymus and the lung (CTs=27-28). Consistent with this lung expression, this transcript was found in the pulmonary mucoepidermoid cell line H292 and was up-regulated upon treatment with the Th2 cytokines IL4 and IL9. This gene was also expressed at lower levels in lung fibroblasts treated with IL4. Modulation of the expression or activity of this gene or gene product by small molecule, protein or antibody therapeutics is beneficial for the treatment of inflammatory lung diseases such as asthma, emphysema and chronic obstructive pulmonary diseases. Therapeutics designed with the gene or the protein encoded for by this transcript are important for maintaining or restoring normal function of thymus during inflammation.


Panel 5 Islet Summary: Ag2505 Highest expression of this gene was detected in uterus (CT=30). Moderate expression of this gene was also seen in adipose and skeletal muscle of gestational diabetic patients both requiring and not requiring daily injections of insulin. This gene was also expressed in samples derived from a pregnant and a nondiabetic, but overweight patient. In addition, this gene was also expressed in islet beta cells (those that are insulin producing) and small intestine. Therefore, therapeutic modulation of this gene or gene product is useful in the treatment of metabolically related diseases including obesity, Type I and Type II diabetes.


general oncology screening panel_v—2.4 Summary: Ag2505 Highest expression of this gene was detected in prostate cancer (CT=27.7). Moderate to low levels of expression of this gene was seen in both normal and cancer samples derived from colon, lung, prostate and kidney. Consistent with panels 2.2 and 2D, expression of this gene was downregulated in kidney cancer as compared to normal kidney. But higher expression of this gene was seen in colon cancer as compared to corresponding normal adjacent sample. Therefore, expression of this gene distinguishes between cancer and normal kidney and colon tissue. Please see panel 1.3, 1.6, 2.2 for further discussion on the utility of this gene.


B. CG50377-01 and CG50377-02 and CG50377-03 and CG50377-04 and CG50377-05 and CG50377-06: (Cub-Sushi)x Domain Containing Protein


Expression of gene CG50377-01 and variants CG50377-02, CG50377-03, CG50377-04, CG50377-05 and CG50377-06 was assessed using the primer-probe sets Ag2420 and Ag5824, described in Tables BA and BB. Results of the RTQ-PCR runs are shown in Tables BC, BD, BE and BF.

TABLE BAProbe Name Ag2420StartSEQ IDPrimersSequencesLengthPositionNoForward5′-ctgcacttggctggaactta-3′2094651216ProbeTET-5′-tttcatctcctactccaggtgtacca-3′-2694981217TAMRAReverse5′-atctccacaggccctgtaat-3′2095251218









TABLE BB










Probe Name Ag5824















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-ccgcttcatctctgatttctc-3′
21
2319
1219






Probe
TET-5′-tgaaggattcaacatcaccttctcag-3′-
26
2349
1220



TAMRA





Reverse
5′-acagggctccaagtcgtact-3′
20
2375
1221
















TABLE BC










General_screening_panel_v1.5










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
6.7



Melanoma* Hs688(B).T
4.6



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.3



Testis Pool
0.5



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.1



Placenta
0.0



Uterus Pool
0.1



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. IGROV-1
0.2



Ovarian ca. OVCAR-8
0.8



Ovary
0.1



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.2



Trachea
0.3



Lung
0.0



Fetal Lung
0.0



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.0



Lung ca. NCI-H526
0.5



Lung ca. NCI-H23
0.3



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.1



Lung ca. NCI-H522
0.8



Liver
0.0



Fetal Liver
0.1



Liver ca. HepG2
0.0



Kidney Pool
0.1



Fetal Kidney
0.9



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
1.6



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.2



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.1



Colon cancer tissue
1.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.1



Small Intestine Pool
0.6



Stomach Pool
0.3



Bone Marrow Pool
0.1



Fetal Heart
0.1



Heart Pool
0.1



Lymph Node Pool
0.2



Fetal Skeletal Muscle
0.3



Skeletal Muscle Pool
0.1



Spleen Pool
0.7



Thymus Pool
0.5



CNS cancer (glio/astro) U87-MG
100.0



CNS cancer (glio/astro) U-118-MG
4.6



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
1.0



CNS cancer (glio) SNB-19
0.6



CNS cancer (glio) SF-295
19.6



Brain (Amygdala) Pool
4.0



Brain (cerebellum)
9.5



Brain (fetal)
86.5



Brain (Hippocampus) Pool
5.4



Cerebral Cortex Pool
5.8



Brain (Substantia nigra) Pool
4.9



Brain (Thalamus) Pool
7.7



Brain (whole)
7.5



Spinal Cord Pool
3.3



Adrenal Gland
1.4



Pituitary gland Pool
0.2



Salivary Gland
0.0



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
0.3









Column A - Rel. Exp. (%) Ag5824, Run 246273272














TABLE BD










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.0



Pancreas
0.9



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
1.1



Thyroid
0.2



Salivary gland
0.0



Pituitary gland
0.4



Brain (fetal)
100.0



Brain (whole)
13.6



Brain (amygdala)
27.4



Brain (cerebellum)
3.9



Brain (hippocampus)
95.3



Brain (substantia nigra)
5.0



Brain (thalamus)
7.9



Cerebral Cortex
37.6



Spinal cord
2.5



glio/astro U87-MG
50.3



glio/astro U-118-MG
2.7



astrocytoma SW1783
10.9



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.8



glioma U251
1.4



glioma SF-295
7.8



Heart (fetal)
0.0



Heart
0.2



Skeletal muscle (fetal)
1.3



Skeletal muscle
0.0



Bone marrow
0.0



Thymus
0.0



Spleen
1.2



Lymph node
0.2



Colorectal
0.0



Stomach
0.2



Small intestine
1.7



Colon ca. SW480
0.3



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
1.1



Colon ca. HCC-2998
0.0



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.6



Trachea
0.2



Kidney
0.0



Kidney (fetal)
0.6



Renal ca. 786-0
0.0



Renal ca. A498
0.2



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
0.2



Liver ca. (hepatoblast) HepG2
0.0



Lung
0.6



Lung (fetal)
0.2



Lung ca. (small cell) LX-1
0.0



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.2



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
0.4



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
1.3



Breast ca. MDA-N
0.0



Ovary
0.5



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0



Uterus
0.0



Placenta
0.2



Prostate
0.4



Prostate ca.* (bone met)PC-3
0.0



Testis
1.3



Melanoma Hs688(A).T
2.4



Melanoma* (met) Hs688(B).T
0.3



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
0.5









Column A - Rel. Exp. (%) Ag2420, Run 159253002














TABLE BE










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
10.7



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.7



Small airway epithelium none
1.3



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
2.4



Coronery artery SMC TNFalpha + IL-1beta
4.5



Astrocytes rest
15.7



Astrocytes TNFalpha + IL-1beta
20.6



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
3.6



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
3.9



Liver cirrhosis
1.6



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
75.8



Lung fibroblast TNF alpha + IL-1 beta
5.4



Lung fibroblast IL-4
28.9



Lung fibroblast IL-9
100.0



Lung fibroblast IL-13
2.9



Lung fibroblast IFN gamma
45.7



Dermal fibroblast CCD1070 rest
52.1



Dermal fibroblast CCD1070 TNF alpha
39.0



Dermal fibroblast CCD1070 IL-1 beta
47.3



Dermal fibroblast IFN gamma
4.6



Dermal fibroblast IL-4
11.8



Dermal Fibroblasts rest
26.4



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.0



Colon
0.0



Lung
0.8



Thymus
0.0



Kidney
0.0









Column A - Rel. Exp. (%) Ag5824, Run 246921321














TABLE BF










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
11.3



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
1.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
3.9



Coronery artery SMC TNFalpha + IL-1 beta
3.2



Astrocytes rest
100.0



Astrocytes TNFalpha + IL-1beta
32.8



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
0.0



Lupus kidney
0.0



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
36.3



Lung fibroblast TNF alpha + IL-1 beta
4.6



Lung fibroblast IL-4
52.9



Lung fibroblast IL-9
78.5



Lung fibroblast IL-13
37.4



Lung fibroblast IFN gamma
37.6



Dermal fibroblast CCD1070 rest
63.3



Dermal fibroblast CCD1070 TNF alpha
27.0



Dermal fibroblast CCD1070 IL-1 beta
20.3



Dermal fibroblast IFN gamma
8.6



Dermal fibroblast IL-4
4.2



IBD Colitis 2
0.0



IBD Crohn's
0.0



Colon
0.7



Lung
0.8



Thymus
1.9



Kidney
0.0









Column A - Rel. Exp. (%) Ag2420, Run 159255381







General_screeningpanel_v1.5 Summary: Ag5824 Highest expression of this gene was detected in a brain cancer cell line (CT=28.2). Significant expression of this gene was also seen in two melanoma and three brain cancer cell lines. Therefore, expression of this gene detects the presence of melanoma and brain cancer. Therapeutic modulation of this gene or its protein product is useful in the treatment of these cancers.


In addition, this gene was moderately expressed in all the regions of central nervous system examined, including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. Therapeutic modulation of this gene or gene product is useful in the treatment of central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Panel 1.1 Summary: Ag575 Expression of this gene was restricted to normal brain derived tissue. In addition, expression was associated with a number of samples derived from brain cancer cell lines, with highest expression seen in the brain cancer cell line U87-MG (CT=23.5). Thus, the expression of this gene distinguishes these brain derived tissues from other samples in the panel. Therapeutic modulation of this gene or gene product, through the use of antibodies, small molecule drugs or protein therapeutics is of benefit in the treatment of brain cancer.


This gene also had moderate levels of expression in a number of metabolic tissues including adrenal, pituitary, heart, and fetal skeletal muscle.


Panel 1.3D Summary: Ag2420 Expression of this gene was restricted to normal brain derived tissue, with highest expression seen in the fetal brain (CT=29.8). In addition, expression was associated with a number of samples derived from brain cancer cell lines. Thus, the expression of this gene distinguishes these brain derived tissues from other samples in the panel. Therapeutic modulation of this gene or gene product, through the use of antibodies, small molecule drugs or protein therapeutics is of benefit in the treatment of brain cancer.


This gene represents a novel protein containing CUB and sushi domains. Its expression profile was highly brain-preferential; levels in the CNS appear 10-fold greater than in other tissues. At least one brain-specific protein containing CUB and sushi domains has been linked to seizures, and shows differential expression in response to pentylentetrazole (Shimizu-Nishikawa K, Brain Res Mol Brain Res 1995 February; 28(2):201-10). This gene or its protein product is a drug target for the treatment of epilepsy or any seizure disorder.


Panel 4.1D Summary: Ag5824 Highest expression of this gene was detected in IL-9 activated lung fibroblast cells (CT=32). Significant expression of this gene was also seen in resting and activated astrocytes, lung fibroblasts and dermal fibroblasts. The expression pattern in this panel was in agreement with that seen in panel 4D. Please see panel 4D for further discussion on the utility of this gene.


Panel 4D Summary: Ag2420 This gene encodes a cub-domain and sushi-domain containing single-pass membrane protein. Highest expression of this gene was seen in resting astrocytes (CT=30.6). Significant expression of this gene was also detected in TNF-alpha+IL-1-beta-stimulated astrocytes, resting and cytokine-stimulated lung fibroblasts and dermal fibroblasts. Therefore, an isolated extracellular domain of this gene or its protein product is useful as a therapeutic protein to reduce or eliminate the symptoms of multiple sclerosis, chronic obstructive pulmonary disease, asthma, or emphysema, and psoriasis.


Agonist or antagonist antibodies that stimulate or inhibit the function of this gene or its protein product are useful as therapeutics to reduce or eliminate the symptoms of multiple sclerosis, chronic obstructive pulmonary disease, asthma, or emphysema, and psoriasis.


C. CG5038904: Interleukin-1 Receptor Related Protein Like


Expression of gene CG50389-04 was assessed using the primer-probe set Ag48 11, described in Table CA. Results of the RTQ-PCR runs are shown in Tables CB and CC.

TABLE CAProbe Name Ag4811StartSEQ IDPrimersSequencesLengthPositionNoForward5′-catgtctcttttcgggaacata-3′229181222ProbeTET-5′-cacagtaaacatcaccttcttggaagtg-3′-289471223TAMRAReverse5′-gaaggccataatcttccatttt-3′229751224









TABLE CB










General_screening_panel_v1.4










Tissue Name
A














Adipose
4.9



Melanoma* Hs688(A).T
1.4



Melanoma* Hs688(B).T
2.9



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
45.4



Testis Pool
1.7



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
2.7



Placenta
0.5



Uterus Pool
0.4



Ovarian ca. OVCAR-3
1.4



Ovarian ca. SK-OV-3
0.3



Ovarian ca. OVCAR-4
45.1



Ovarian ca. OVCAR-5
0.0



Ovarian ca. IGROV-1
0.4



Ovarian ca. OVCAR-8
25.9



Ovary
6.0



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
32.1



Breast ca. BT 549
10.9



Breast ca. T47D
1.2



Breast ca. MDA-N
0.0



Breast Pool
6.3



Trachea
8.1



Lung
0.1



Fetal Lung
5.3



Lung ca. NCI-N417
0.0



Lung ca. LX-1
3.9



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.1



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
3.8



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.1



Lung ca. NCI-H522
0.0



Liver
1.2



Fetal Liver
4.3



Liver ca. HepG2
0.0



Kidney Pool
3.2



Fetal Kidney
5.1



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
5.3



Renal ca. TK-10
71.7



Bladder
6.5



Gastric ca. (liver met.) NCI-N87
100.0



Gastric ca. KATO III
24.1



Colon ca. SW-948
2.4



Colon ca. SW480
7.1



Colon ca.* (SW480 met) SW620
0.5



Colon ca. HT29
16.8



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.5



Colon cancer tissue
12.1



Colon ca. SW1116
6.9



Colon ca. Colo-205
3.7



Colon ca. SW-48
0.0



Colon Pool
1.6



Small Intestine Pool
1.2



Stomach Pool
7.0



Bone Marrow Pool
3.1



Fetal Heart
0.4



Heart Pool
1.3



Lymph Node Pool
2.8



Fetal Skeletal Muscle
0.6



Skeletal Muscle Pool
3.0



Spleen Pool
4.2



Thymus Pool
3.8



CNS cancer (glio/astro) U87-MG
0.2



CNS cancer (glio/astro) U-118-MG
0.8



CNS cancer (neuro; met) SK-N-AS
0.3



CNS cancer (astro) SF-539
0.4



CNS cancer (astro) SNB-75
4.9



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala) Pool
4.6



Brain (cerebellum)
0.4



Brain (fetal)
4.7



Brain (Hippocampus) Pool
3.0



Cerebral Cortex Pool
6.9



Brain (Substantia nigra) Pool
4.9



Brain (Thalamus) Pool
9.2



Brain (whole)
7.0



Spinal Cord Pool
0.8



Adrenal Gland
12.5



Pituitary gland Pool
0.1



Salivary Gland
2.8



Thyroid (female)
6.3



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
21.0









Column A - Rel. Exp. (%) Ag4811, Run 223204488














TABLE CC










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
1.5



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.9



Primary Th2 act
5.1



Primary Tr1 act
2.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
3.3



CD8 lymphocyte act
0.7



Secondary CD8 lymphocyte rest
2.9



Secondary CD8 lymphocyte act
0.7



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
3.9



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
1.2



LAK cells IL-2 + IL-18
0.7



LAK cells PMA/ionomycin
11.1



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.7



Two Way MLR 7 day
1.6



PBMC rest
0.0



PBMC PWM
4.8



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
10.7



B lymphocytes CD40L and IL-4
0.8



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
2.7



Dendritic cells none
9.4



Dendritic cells LPS
0.6



Dendritic cells anti-CD40
15.5



Monocytes rest
0.0



Monocytes LPS
8.8



Macrophages rest
1.6



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
1.7



HUVEC IFN gamma
2.3



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
8.5



Small airway epithelium none
11.1



Small airway epithelium TNFalpha + IL-1beta
14.9



Coronery artery SMC rest
3.1



Coronery artery SMC TNFalpha + IL-1beta
1.4



Astrocytes rest
2.2



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
100.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
89.5



Liver cirrhosis
4.6



NCI-H292 none
2.4



NCI-H292 IL-4
5.0



NCI-H292 IL-9
1.9



NCI-H292 IL-13
6.0



NCI-H292 IFN gamma
3.9



HPAEC none
2.4



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
26.1



Lung fibroblast TNF alpha + IL-1 beta
17.1



Lung fibroblast IL-4
33.9



Lung fibroblast IL-9
27.7



Lung fibroblast IL-13
25.9



Lung fibroblast IFN gamma
52.1



Dermal fibroblast CCD1070 rest
9.8



Dermal fibroblast CCD1070 TNF alpha
5.4



Dermal fibroblast CCD1070 IL-1 beta
4.3



Dermal fibroblast IFN gamma
5.6



Dermal fibroblast IL-4
14.9



Dermal Fibroblasts rest
7.5



Neutrophils TNFa + LPS
54.7



Neutrophils rest
0.9



Colon
0.8



Lung
4.4



Thymus
4.5



Kidney
29.9









Column A - Rel. Exp. (%) Ag4811, Run 223273603







General_screeningpanel_v1.4 Summary: Ag4811 The highest expression of this gene was detected in a sample derived from a gastric cancer cell line (NCI-N87) (CT=29.2). There was low to moderate expression associated with other gastric cancer cell lines, colon cancer cell lines, breast cancer cell lines, ovarian cancer cell lines and a renal cancer cell line. The expression of this gene or protein product is useful as a marker for gastric cancer, colon cancer, breast cancer, ovarian cancer or renal cancer. Therapeutic modulation of this gene or its protein product through the use of antibodies, protein therapeutics or small molecule drugs is useful in the treatment of gastric cancer, colon cancer, breast cancer, ovarian cancer or renal cancer.


This gene was also moderately expressed in a number of metabolic tissues including adipose, fetal liver, skeletal muscle, adrenal, thyroid and pancreas. Therapeutic modulation of this gene, expressed protein and/or use of antibodies, protein therapeutics or small molecule drugs targeting the gene or gene product are useful in the treatment of metabolic diseases, including obesity and Types 1 and 2 diabetes.


This gene product was expressed at low levels in the brain. This gene encodes a protein with homology to an interleukin 1 receptor-like protein. Interleukins are involved in many brain pathologies due to the pro-inflammatory action of interleukins. Inflammation is a key pathological process mediating the damage seen in many disease states including stroke, Alzheimer's disease, and spinocerebellar ataxias (J Neurosci 2001 August 1;21(15):5389-96). IL-1 receptor like protein has been shown to bind IL-18 (J Immunol 1999 May 1;162(9):5041-4), which is involved in CNS inflammation. Moreover, anti-inflammatory agents that are associated with reduced risk of Alzheimer's Disease, such as NSAIDS (Drug Saf 2001;24(11):801-11), have been shown to inhibit IL-18 expression. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drugs targeting the gene or gene product are useful in the treatment of stroke, Alzheimer's disease, and spinocerebellar ataxias and any other CNS disorder in which inflammation plays a role.


Panel 4.1D Summary: Ag 4811 The highest expression of this gene was detected in keratinocytes, regardless of their treatment. This transcript encodes a novel IL-1 receptor related protein that may have the potential to trigger novel members of the IL-1 family members as described by Debets et. al. (J Immunol 2001 August 1;167(3):1440-6; J Biol Chem 1996 February 23;271(8):3967-70). Novel IL1 receptor like molecules have been cloned and reported to lead to activation of NF-KB and IL18 production, a potent inflammatory cytokine associated with lung inflammation, IBD and psoriasis. IL1 R family members have also been shown to mediate inflammatory signals through NF-kB activation, among other signalling pathways. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drugs targeting the gene or gene product are useful in the treatment of inflammatory lung and skin diseases such as asthma, bronchitis, emphysema and psoriasis.


This gene was also expressed at moderate levels in neutrophils stimulated with TNF-a and LPS. The expression of this transcript in neutrophils demonstrates an important role of this receptor in innate immunity and in inflammation associated with neutrophil accummulation such as observed in inflammatory bowel diseases, psoriasis, asthma, chronic bronchitis and rheumatoid arthritis. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drugs targeting the gene or gene product are useful in the treatment of inflammatory disorders including inflammatory bowel diseases, psoriasis, asthma, chronic bronchitis and rheumatoid arthritis.


D. CG50389-06: Interleukin-1 Receptor Related Protein


Expression of full-length physical clone CG50389-06 was assessed using the primer-probe sets Ag4944 and Ag4945, described in Tables DA and DB. Results of the RTQ-PCR runs are shown in Table DC.

TABLE DAProbe Name Ag4944StartSEQ IDPrimersSequencesLengthPositionNoForward5′-cagggaagcagtacgaggttt-3′2111611225ProbeTET-5′-tggaggaagtgtccctaaaatcatttatcc-30109112263′-TAMRAReverse5′-tgactctccagcatcgtaga-3′209901227










TABLE DB










Probe Name Ag4945
















Start
SEQ ID



Primers
Sequences
Length
Positon
No















Forward
5′-cagtgagataccacatgccg-3′
20
175
1228






Probe
TET-5′-agctgccgcagcacacaccttgctac-
26
118
1229



3′-TAMRA





Reverse
5′-gtcatcttccagccataggtgt-3′
22
22
1230
















TABLE DC










Panel 4.1D









Tissue Name
A
B












Secondary Th1 act
0.0
52.1


Secondary Th2 act
0.0
0.0


Secondary Tr1 act
0.0
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
32.3


Secondary Tr1 rest
0.0
64.2


Primary Th1 act
0.0
12.9


Primary Th2 act
0.0
18.3


Primary Tr1 act
0.0
0.0


Primary Th1 rest
0.0
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
0.0
17.1


CD45RA CD4 lymphocyte act
0.0
34.2


CD45RO CD4 lymphocyte act
0.0
0.0


CD8 lymphocyte act
0.0
50.7


Secondary CD8 lymphocyte rest
0.0
20.2


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
12.9


LAK cells rest
0.0
20.0


LAK cells IL-2
0.0
0.0


LAK cells IL-2 + IL-12
0.0
43.5


LAK cells IL-2 + IFN gamma
0.0
21.6


LAK cells IL-2 + IL-18
0.0
49.0


LAK cells PMA/ionomycin
7.7
40.3


NK Cells IL-2 rest
0.0
61.1


Two Way MLR 3 day
0.0
12.4


Two Way MLR 5 day
0.0
11.8


Two Way MLR 7 day
0.0
0.0


PBMC rest
0.0
21.3


PBMC PWM
0.0
48.0


PBMC PHA-L
0.0
25.3


Ramos (B cell) none
0.0
0.0


Ramos (B cell) ionomycin
0.0
16.6


B lymphocytes PWM
10.0
35.1


B lymphocytes CD40L and IL-4
0.0
0.0


EOL-1 dbcAMP
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
30.4


Dendritic cells none
6.9
0.0


Dendritic cells LPS
0.0
40.3


Dendritic cells anti-CD40
6.2
0.0


Monocytes rest
0.0
47.6


Monocytes LPS
0.0
0.0


Macrophages rest
0.0
27.7


Macrophages LPS
0.0
8.9


HUVEC none
0.0
27.9


HUVEC starved
0.0
17.3


HUVEC IL-1beta
0.0
14.7


HUVEC IFN gamma
0.0
17.2


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
0.0
58.6


HUVEC IL-11
0.0
35.1


Lung Microvascular EC none
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
0.0
37.9


Microvascular Dermal EC none
0.0
19.9


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.0


Bronchial epithelium TNFalpha + IL1beta
10.7
10.1


Small airway epithelium none
2.7
44.8


Small airway epithelium TNFalpha + IL-1beta
8.1
33.4


Coronery artery SMC rest
0.0
18.7


Coronery artery SMC TNFalpha + IL-1beta
0.0
34.9


Astrocytes rest
0.0
18.6


Astrocytes TNFalpha + IL-1beta
0.0
12.4


KU-812 (Basophil) rest
0.0
18.9


KU-812 (Basophil) PMA/ionomycin
0.0
0.0


CCD1106 (Keratinocytes) none
99.3
68.8


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
100.0
21.6


Liver cirrhosis
0.0
0.0


NCI-H292 none
5.7
48.3


NCI-H292 IL-4
0.0
19.9


NCI-H292 IL-9
3.1
20.4


NCI-H292 IL-13
8.0
0.0


NCI-H292 IFN gamma
3.2
0.0


HPAEC none
0.0
55.9


HPAEC TNF alpha + IL-1 beta
0.0
19.1


Lung fibroblast none
3.9
74.7


Lung fibroblast TNF alpha + IL-1 beta
10.2
48.3


Lung fibroblast IL-4
19.5
44.8


Lung fibroblast IL-9
31.9
56.6


Lung fibroblast IL-13
23.8
0.0


Lung fibroblast IFN gamma
33.2
37.9


Dermal fibroblast CCD1070 rest
0.0
100.0


Dermal fibroblast CCD1070 TNF alpha
4.5
24.8


Dermal fibroblast CCD1070 IL-1 beta
0.0
23.2


Dermal fibroblast IFN gamma
0.0
11.8


Dermal fibroblast IL-4
12.2
46.0


Dermal Fibroblasts rest
3.8
0.0


Neutrophils TNFa + LPS
23.5
51.8


Neutrophils rest
2.5
0.0


Colon
0.0
0.0


Lung
0.0
29.1


Thymus
0.0
8.9


Kidney
15.3
39.2







Column A - Rel. Exp. (%) Ag4944, Run 223598842





Column B - Rel. Exp. (%) Ag4945, Run 223625870







Panel 4.1D Summary: 4845/4944 Highest expression of the CG50389-06 transcript was seen in keratinocytes, regardless of their treatment with TNF-a plus IL-1b.


It was also found to a lesser extent in neutrophils and lung fibroblasts. This transcript encodes for a novel IL-1 receptor related protein that may have the potential to trigger novel members of the IL-1 family members as described by Debets and Parnet. (J Immunol Aug. 1, 2001; 167(3):1440-6; J Biol Chem. Feb. 23, 1996; 271(8):3967-70 ). Novel IL1 receptor like molecules have been cloned and reported to lead to activation of NF-kB and IL18 production, a potent inflammatory cytokine associated with lung inflammation, IBD and psoriasis. IL1 R family members have also been shown to mediate inflammatory signals through NF-kB activation, among other signalling pathways. Modulation of the function of this receptor by the use of protein therapeutics or antibodies prevents or reduces the severity of inflammatory processes observed in inflammatory lung and skin diseases such as asthma, bronchitis, emphysema and psoriasis.


E. CG50391-08 and CG50391-09 and CG50391-01:


Expression of gene CG50391-08 was assessed using the primer-probe sets Ag2654, Ag2726, and Ag2826, described in Tables EA, EB, and EC. Results of the RTQ-PCR runs are shown in Tables ED, EE, EF and EG.

TABLE EAProbe Name Ag2654PrimerSequencesLengthStart PositionSEQ ID NoForward5′-tagaggagctgctcagcaaata-3′222971231ProbeTET-5′-cacaacgagtctcactcccgggt-3′-TAMRA233231232Reverse5′-catgaggatctcctccttgtc-3′213681233









TABLE EB










Probe Name Ag2726











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-tagaggagctgctcagcaaata-3′
22
297
1234






Probe
TET-5′-cacaacgagtctcactcccgggt-3′-TAMRA
23
323
1235





Reverse
5′-catgaggatctcctccttgtc-3′
21
368
1236
















TABLE EC










Probe Name Ag2826











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-ccctcttactctagcccatttg-3′
22
2108
1237






Probe
TET-5′-tgtctgaccagcaaacattaaaacca-3′-TAMRA
26
2080
1238





Reverse
5′-acagtccgtccctaccaataga-3′
22
2052
1239
















TABLE ED










Panel 1.3D











Tissue Name
A
B
C














Liver adenocarcinoma
5.9
3.9
4.5



Pancreas
1.7
1.5
1.1


Pancreatic ca. CAPAN 2
0.1
0.2
0.2


Adrenal gland
10.4
10.7
10.5


Thyroid
6.3
6.9
5.6


Salivary gland
1.0
2.5
2.6


Pituitary gland
1.8
1.8
1.4


Brain (fetal)
0.2
0.4
0.3


Brain (whole)
1.6
1.4
0.9


Brain (amygdala)
1.6
1.8
1.7


Brain (cerebellum)
0.5
0.8
0.5


Brain (hippocampus)
6.2
6.3
5.0


Brain (substantia nigra)
1.5
1.9
0.5


Brain (thalamus)
1.4
1.4
2.0


Cerebral Cortex
0.9
1.4
1.6


Spinal cord
8.3
6.0
6.0


glio/astro U87-MG
0.0
0.1
0.0


glio/astro U-118-MG
4.0
3.1
2.5


astrocytoma SW1783
13.0
12.6
9.7


neuro*; met SK-N-AS
1.3
1.3
0.5


astrocytoma SF-539
0.4
0.1
0.7


astrocytoma SNB-75
5.7
5.8
4.2


glioma SNB-19
0.2
0.0
0.0


glioma U251
0.0
0.1
0.1


glioma SF-295
2.4
2.4
0.8


Heart (fetal)
17.4
19.1
9.7


Heart
5.7
6.0
4.9


Skeletal muscle (fetal)
100.0
100.0
100.0


Skeletal muscle
0.6
0.4
0.6


Bone marrow
5.0
6.3
5.1


Thymus
3.6
2.8
2.4


Spleen
8.8
9.1
11.3


Lymph node
4.7
5.6
3.8


Colorectal
27.9
29.7
44.4


Stomach
8.7
12.9
12.2


Small intestine
16.6
8.7
23.3


Colon ca. SW480
2.0
2.5
1.4


Colon ca.* SW620(SW480 met)
0.0
0.1
0.1


Colon ca. HT29
0.3
0.0
0.2


Colon ca. HCT-116
0.4
0.7
0.6


Colon ca. CaCo-2
0.2
0.7
0.4


Colon ca. tissue(ODO3866)
20.0
24.7
17.2


Colon ca. HCC-2998
0.3
0.6
0.7


Gastric ca.* (liver met) NCI-N87
0.3
0.6
1.4


Bladder
14.2
17.8
7.9


Trachea
13.6
11.9
7.6


Kidney
1.3
1.6
1.1


Kidney (fetal)
8.1
9.0
4.7


Renal ca. 786-0
2.4
4.4
2.4


Renal ca. A498
4.0
7.2
4.7


Renal ca. RXF 393
4.2
6.3
5.7


Renal ca. ACHN
79.0
79.0
38.7


Renal ca. UO-31
15.4
17.0
6.9


Renal ca. TK-10
0.3
0.3
0.1


Liver
1.1
1.0
0.7


Liver (fetal)
2.7
5.3
4.5


Liver ca. (hepatoblast) HepG2
0.0
0.0
0.0


Lung
25.0
24.5
27.4


Lung (fetal)
29.1
26.1
22.2


Lung ca. (small cell) LX-1
0.8
0.0
1.1


Lung ca. (small cell) NCI-H69
0.4
0.0
0.8


Lung ca. (s. cell var.) SHP-77
2.7
4.6
1.8


Lung ca. (large cell) NCI-H460
0.1
0.0
0.0


Lung ca. (non-sm. cell) A549
0.1
0.2
0.1


Lung ca. (non-s. cell) NCI-H23
0.4
0.7
0.4


Lung ca. (non-s. cell) HOP-62
0.8
1.6
0.5


Lung ca. (non-s. cl) NCI-H522
0.1
0.2
0.0


Lung ca. (squam.) SW 900
0.8
1.3
2.4


Lung ca. (squam.) NCI-H596
0.1
0.0
0.5


Mammary gland
16.7
11.7
10.8


Breast ca.* (pl. ef) MCF-7
0.9
0.0
0.1


Breast ca.* (pl. ef) MDA-MB-231
3.3
5.2
2.9


Breast ca.* (pl. ef) T47D
1.4
0.5
2.8


Breast ca. BT-549
2.6
2.4
2.3


Breast ca. MDA-N
0.0
0.0
0.0


Ovary
28.1
28.3
47.6


Ovarian ca. OVCAR-3
7.0
7.6
5.5


Ovarian ca. OVCAR-4
0.0
0.4
0.2


Ovarian ca. OVCAR-5
2.1
1.4
1.3


Ovarian ca. OVCAR-8
0.3
0.4
0.0


Ovarian ca. IGROV-1
0.1
0.1
0.0


Ovarian ca.* (ascites) SK-OV-3
0.0
0.1
0.0


Uterus
26.2
23.3
22.4


Placenta
27.4
29.1
32.5


Prostate
29.5
33.2
38.2


Prostate ca.* (bone met)PC-3
0.1
0.0
0.1


Testis
13.4
12.2
10.5


Melanoma Hs688(A).T
47.0
42.0
20.7


Melanoma* (met) Hs688(B).T
3.6
4.5
4.2


Melanoma UACC-62
0.0
0.1
0.1


Melanoma M14
0.0
0.1
0.0


Melanoma LOX IMVI
2.3
0.0
0.0


Melanoma* (met) SK-MEL-5
0.1
0.5
0.0


Adipose
81.8
77.9
44.8







Column A - Rel. Exp. (%) Ag2654, Run 153009856





Column B - Rel. Exp. (%) Ag2726, Run 153622446





Column C - Rel. Exp. (%) Ag2826, Run 153774039














TABLE EE










Panel 2D












Tissue Name
A
B
C
















Normal Colon
52.1
75.3
58.2



CC Well to Mod Diff (ODO3866)
18.7
19.5
15.2



CC Margin (ODO3866)
18.4
18.4
13.2



CC Gr.2 rectosigmoid
8.2
10.4
2.8



(ODO3868)



CC Margin (ODO3868)
9.0
8.2
2.3



CC Mod Diff (ODO3920)
8.7
9.2
3.6



CC Margin (ODO3920)
20.2
20.9
5.0



CC Gr.2 ascend colon
16.4
17.9
15.9



(ODO3921)



CC Margin (ODO3921)
16.4
18.2
11.0



CC from Partial Hepatectomy
14.4
11.7
10.4



(ODO4309) Mets



Liver Margin (ODO4309)
8.9
6.0
7.9



Colon mets to lung (OD04451-01)
16.5
12.2
4.5



Lung Margin (OD04451-02)
33.9
28.3
9.9



Normal Prostate 6546-1
66.0
45.1
51.8



Prostate Cancer (OD04410)
30.4
30.1
31.9



Prostate Margin (OD04410)
75.8
82.9
67.8



Prostate Cancer (OD04720-01)
58.2
47.6
31.0



Prostate Margin (OD04720-02)
51.1
46.3
31.0



Normal Lung 061010
16.4
17.9
20.4



Lung Met to Muscle (ODO4286)
4.5
2.8
2.5



Muscle Margin (ODO4286)
22.2
20.7
10.3



Lung Malignant Cancer
10.4
6.4
9.4



(OD03126)



Lung Margin (OD03126)
15.8
16.0
12.7



Lung Cancer (OD04404)
16.6
14.1
11.8



Lung Margin (OD04404)
87.7
54.3
40.1



Lung Cancer (OD04565)
15.2
14.5
7.7



Lung Margin (OD04565)
17.8
13.8
3.5



Lung Cancer (OD04237-01)
5.9
7.2
11.8



Lung Margin (OD04237-02)
64.6
48.3
43.8



Ocular Mel Met to Liver
0.4
0.7
0.6



(ODO4310)



Liver Margin (ODO4310)
12.6
10.6
9.8



Melanoma Mets to Lung
1.6
1.1
1.1



(OD04321)



Lung Margin (OD04321)
32.8
33.2
32.8



Normal Kidney
21.0
17.6
25.7



Kidney Ca, Nuclear grade 2
33.7
31.6
22.4



(OD04338)



Kidney Margin (OD04338)
48.0
39.5
14.4



Kidney Ca Nuclear grade 1/2
3.1
3.1
1.4



(OD04339)



Kidney Margin (OD04339)
9.3
7.6
4.5



Kidney Ca, Clear cell type
27.4
23.7
17.0



(OD04340)



Kidney Margin (OD04340)
34.9
28.3
19.1



Kidney Ca, Nuclear grade 3
9.3
8.0
5.3



(OD04348)



Kidney Margin (OD04348)
27.0
23.2
10.9



Kidney Cancer (OD04622-01)
12.7
11.6
6.9



Kidney Margin (OD04622-03)
8.4
7.4
4.5



Kidney Cancer (OD04450-01)
10.6
6.4
8.5



Kidney Margin (OD04450-03)
29.7
24.8
8.2



Kidney Cancer 8120607
3.6
4.9
2.5



Kidney Margin 8120608
12.8
16.5
6.8



Kidney Cancer 8120613
0.4
0.0
0.1



Kidney Margin 8120614
9.9
9.4
5.4



Kidney Cancer 9010320
35.4
33.4
18.7



Kidney Margin 9010321
27.5
24.8
11.3



Normal Uterus
20.4
24.1
12.9



Uterus Cancer 064011
50.0
48.6
12.2



Normal Thyroid
11.4
7.2
10.4



Thyroid Cancer 064010
1.6
2.1
2.5



Thyroid Cancer A302152
13.8
12.5
3.5



Thyroid Margin A302153
9.0
7.7
9.9



Normal Breast
33.2
32.5
14.9



Breast Cancer (OD04566)
9.9
8.5
2.1



Breast Cancer (OD04590-01)
7.5
9.9
9.6



Breast Cancer Mets
14.2
14.7
12.7



(OD04590-03)



Breast Cancer Metastasis
2.5
1.8
2.0



(OD04655-05)



Breast Cancer 064006
20.7
19.8
18.4



Breast Cancer 1024
15.4
16.2
12.9



Breast Cancer 9100266
12.0
11.7
18.2



Breast Margin 9100265
15.2
15.2
21.8



Breast Cancer A209073
10.7
10.2
12.8



Breast Margin A209073
7.7
11.1
8.2



Normal Liver
2.1
2.0
1.9



Liver Cancer 064003
1.1
1.0
1.4



Liver Cancer 1025
5.2
5.3
2.6



Liver Cancer 1026
7.3
7.6
7.7



Liver Cancer 6004-T
9.3
11.6
3.4



Liver Tissue 6004-N
3.8
3.8
2.6



Liver Cancer 6005-T
10.2
10.7
8.0



Liver Tissue 6005-N
4.1
3.9
2.1



Normal Bladder
37.1
43.2
35.1



Bladder Cancer 1023
11.3
9.2
11.2



Bladder Cancer A302173
4.4
5.6
10.2



Bladder Cancer (OD04718-01)
28.1
30.6
27.2



Bladder Normal Adjacent
100.0
100.0
78.5



(OD04718-03)



Normal Ovary
10.4
8.1
10.2



Ovarian Cancer 064008
92.7
95.9
100.0



Ovarian Cancer (OD04768-07)
1.9
1.7
1.4



Ovary Margin (OD04768-08)
71.2
68.3
32.8



Normal Stomach
70.7
69.7
43.5



Gastric Cancer 9060358
12.8
16.5
24.3



Stomach Margin 9060359
23.7
25.2
21.2



Gastric Cancer 9060395
25.2
32.5
32.5



Stomach Margin 9060394
43.2
49.0
55.9



Gastric Cancer 9060397
28.1
26.4
29.3



Stomach Margin 9060396
8.7
10.2
9.2



Gastric Cancer 064005
18.6
26.4
33.9









Column A - Rel. Exp. (%) Ag2654, Run 153009865






Column B - Rel. Exp. (%) Ag2726, Run 153622484






Column C - Rel. Exp. (%) Ag2826, Run 153774078














TABLE EF










Panel 4D












Tissue Name
A
B
C
















Secondary Th1 act
0.3
0.5
0.0



Secondary Th2 act
0.0
0.0
0.0



Secondary Tr1 act
0.1
0.0
0.0



Secondary Th1 rest
0.0
0.0
0.0



Secondary Th2 rest
0.1
0.1
0.0



Secondary Tr1 rest
0.0
0.0
0.0



Primary Th1 act
0.1
0.0
0.0



Primary Th2 act
0.1
0.1
0.0



Primary Tr1 act
0.0
0.0
0.0



Primary Th1 rest
0.1
0.2
0.2



Primary Th2 rest
0.0
0.1
0.1



Primary Tr1 rest
0.0
0.0
0.0



CD45RA CD4 lymphocyte act
24.7
20.9
15.3



CD45RO CD4 lymphocyte act
0.2
0.1
0.1



CD8 lymphocyte act
0.0
0.1
0.0



Secondary CD8 lymphocyte rest
0.0
0.0
0.0



Secondary CD8 lymphocyte act
0.3
0.3
0.0



CD4 lymphocyte none
0.4
0.1
0.2



2ry Th1/Th2/Tr1 anti-CD95 CH11
0.0
0.2
0.0



LAK cells rest
1.4
2.7
2.0



LAK cells IL-2
0.2
0.2
0.0



LAK cells IL-2 + IL-12
0.1
0.3
0.1



LAK cells IL-2 + IFN gamma
0.4
0.3
0.2



LAK cells IL-2 + IL-18
0.4
0.0
0.2



LAK cells PMA/ionomycin
2.2
1.0
1.0



NK Cells IL-2 rest
0.0
0.4
0.0



Two Way MLR 3 day
2.2
3.6
1.9



Two Way MLR 5 day
0.8
1.7
0.8



Two Way MLR 7 day
0.1
0.4
0.1



PBMC rest
3.0
3.6
3.9



PBMC PWM
3.8
3.1
4.1



PBMC PHA-L
4.3
3.6
5.9



Ramos (B cell) none
0.9
1.2
2.5



Ramos (B cell) ionomycin
1.0
1.1
3.5



B lymphocytes PWM
0.3
0.5
0.0



B lymphocytes CD40L and IL-4
0.6
0.4
0.1



EOL-1 dbcAMP
0.2
0.1
0.1



EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.1



Dendritic cells none
0.2
0.2
0.2



Dendritic cells LPS
0.4
0.5
0.3



Dendritic cells anti-CD40
0.0
0.1
0.0



Monocytes rest
31.0
31.9
33.4



Monocytes LPS
1.4
2.2
1.3



Macrophages rest
0.1
0.7
0.6



Macrophages LPS
4.8
6.3
5.3



HUVEC none
0.0
0.0
0.0



HUVEC starved
0.3
0.0
0.0



HUVEC IL-1beta
0.0
0.0
0.0



HUVEC IFN gamma
0.1
0.6
0.1



HUVEC TNF alpha + IFN gamma
0.0
0.0
0.0



HUVEC TNF alpha + IL4
0.0
0.0
0.0



HUVEC IL-11
0.2
0.0
0.0



Lung Microvascular EC none
0.0
0.0
0.0



Lung Microvascular EC
0.0
0.1
0.0



TNFalpha + IL-1beta



Microvascular Dermal EC none
1.4
1.6
1.1



Microsvasular Dermal EC
0.1
0.0
0.1



TNFalpha + IL-1 beta



Bronchial epithelium TNFalpha +
0.0
0.0
0.1



IL1beta



Small airway epithelium none
1.6
1.0
1.6



Small airway epithelium
2.2
3.5
6.4



TNFalpha + IL-1beta



Coronery artery SMC rest
0.3
0.3
0.5



Coronery artery SMC TNFalpha +
0.1
0.4
0.0



IL-1beta



Astrocytes rest
18.9
20.6
21.2



Astrocytes TNFalpha + IL-1beta
9.0
8.3
11.6



KU-812 (Basophil) rest
0.0
0.1
0.1



KU-812 (Basophil) PMA/ionomycin
0.6
0.0
0.1



CCD1106 (Keratinocytes) none
0.3
0.4
0.5



CCD1106 (Keratinocytes)
0.0
0.0
0.1



TNFalpha + IL-1 beta



Liver cirrhosis
18.3
22.4
12.5



Lupus kidney
10.3
9.6
9.9



NCI-H292 none
0.5
0.3
1.3



NCI-H292 IL-4
0.7
0.3
1.4



NCI-H292 IL-9
0.5
0.7
1.9



NCI-H292 IL-13
0.6
0.1
0.7



NCI-H292 IFN gamma
0.5
0.4
0.4



HPAEC none
0.0
0.0
0.0



HPAEC TNF alpha + IL-1 beta
0.0
0.4
0.0



Lung fibroblast none
12.0
12.2
10.4



Lung fibroblast TNF alpha + IL-1
8.3
9.7
11.5



beta



Lung fibroblast IL-4
17.8
16.4
25.0



Lung fibroblast IL-9
16.3
16.2
16.8



Lung fibroblast IL-13
14.2
13.9
13.9



Lung fibroblast IFN gamma
29.9
33.0
40.9



Dermal fibroblast CCD1070 rest
89.5
100.0
100.0



Dermal fibroblast CCD1070 TNF
63.3
51.4
76.8



alpha



Dermal fibroblast CCD1070 IL-1
100.0
94.0
70.7



beta



Dermal fibroblast IFN gamma
9.7
9.8
7.9



Dermal fibroblast IL-4
2.8
1.6
1.8



IBD Colitis 2
22.8
30.4
22.4



IBD Crohn's
11.2
14.7
10.4



Colon
18.2
22.1
16.6



Lung
51.8
46.3
40.6



Thymus
11.2
10.3
11.9



Kidney
10.6
8.7
8.0









Column A - Rel. Exp. (%) Ag2654, Run 153594146






Column B - Rel. Exp. (%) Ag2726, Run 153622627






Column C - Rel. Exp. (%) Ag2826, Run 153774117














TABLE EG










Panel 5D











Tissue Name
A
B















97457_Patient-02go_adipose
100.0
74.7



97476_Patient-07sk_skeletal muscle
37.4
31.9



97477_Patient-07ut_uterus
83.5
95.3



97478_Patient-07pl_placenta
22.4
22.5



97481_Patient-08sk_skeletal muscle
12.2
8.2



97482_Patient-08ut_uterus
46.3
48.6



97483_Patient-08pl_placenta
19.5
22.5



97486_Patient-09sk_skeletal muscle
5.8
7.5



97487_Patient-09ut_uterus
19.9
18.7



97488_Patient-09pl_placenta
13.2
24.8



97492_Patient-10ut_uterus
29.7
19.9



97493_Patient-10pl_placenta
31.0
30.4



97495_Patient-11go_adipose
45.1
31.4



97496_Patient-11sk_skeletal muscle
11.0
5.0



97497_Patient-11ut_uterus
66.9
57.0



97498_Patient-11pl_placenta
11.5
21.3



97500_Patient-12go_adipose
62.0
54.7



97501_Patient-12sk_skeletal muscle
15.5
25.9



97502_Patient-12ut_uterus
90.1
62.0



97503_Patient-12pl_placenta
11.6
11.6



94721_Donor 2 U-
31.9
10.9



A_Mesenchymal Stem Cells



94722_Donor 2 U -
15.6
16.6



B_Mesenchymal Stem Cells



94723_Donor 2 U -
18.6
25.3



C_Mesenchymal Stem Cells



94709_Donor 2 AM - A_adipose
81.8
100.0



94710_Donor 2 AM - B_adipose
77.4
84.1



94711_Donor 2 AM - C_adipose
66.4
33.9



94712_Donor 2 AD - A_adipose
15.5
14.8



94713_Donor 2 AD - B_adipose
16.6
18.8



94714_Donor 2 AD - C_adipose
22.5
22.5



94742_Donor 3 U - A_Mesenchymal
33.9
21.6



Stem Cells



94743_Donor 3 U - B_Mesenchymal
27.0
25.2



Stem Cells



94730_Donor 3 AM - A_adipose
75.3
65.1



94731_Donor 3 AM - B_adipose
52.1
80.1



94732_Donor 3 AM - C_adipose
42.0
35.6



94733_Donor 3 AD - A_adipose
12.1
9.9



94734_Donor 3 AD - B_adipose
0.2
14.5



94735_Donor 3 AD - C_adipose
10.7
13.0



77138_Liver_HepG2untreated
1.3
2.6



73556_Heart_Cardiac stromal cells
0.2
0.0



(primary)



81735_Small Intestine
21.0
27.2



72409_Kidney_Proximal Convoluted
2.7
2.8



Tubule



82685_Small intestine_Duodenum
10.2
13.1



90650_Adrenal_Adrenocortical
11.0
15.8



adenoma



72410_Kidney_HRCE
25.2
56.3



72411_Kidney_HRE
7.6
7.0



73139_Uterus_Uterine smooth muscle
1.3
3.1



cells









Column A - Rel. Exp. (%) Ag2654, Run 169270118






Column B - Rel. Exp. (%) Ag2726, Run 169315037







Panel 1.3D Summary: Ag2654/Ag2726/Ag2826 Highest expression of this gene was detected in fetal skeletal muscle (CTs=27-28.2). This gene was expressed at much higher levels in fetal when compared to adult skeletal muscle (CT=35). The relative overexpression of this gene in fetal skeletal muscle demonstrates that the gene or protein product enhances muscular growth or development in the fetus and also acts in a regenerative capacity in the adult.


Moderate to low levels of expression of this gene were also seen in tissues with - metabolic/endocrine-functions-including pancreas,- adipose, adrenal gland, thyroid, pituitary gland, skeletal muscle, heart, liver and the gastrointestinal tract. -Therapeutic modulation of the activity of this gene or gene product is useful in the treatment of endocrine/metabolically related diseases, such as obesity and diabetes.


Moderate expression of this gene was also seen in number of cell lines derived from melanoma, liver, renal, breast, lung, ovarian, and brain cancers.


Panel 2D Summary: Ag2654/Ag2726/Ag2826 Highest expression of this gene was detected in a normal bladder sample (CTs=26-27.5). Significant expression of this gene was seen in both normal and cancer samples used in this panel. Expression of this gene was higher in normal lung, bladder and breast samples as compared to their corresponding cancer samples.


Panel 4D Summary: Ag2654/Ag2726/Ag2826 Highest expression of this gene was detected in dermal fibroblasts (CTs=26.4-28). High to moderate expression of this gene was detected in dermal and lung fibroblasts, activated CD45RA CD4 lymphocyte cells, two way MLR, PBMC cells, resting monocytes, LPS treated macrophage, small airway epithelium, liver cirrhosis, lupus kidney, IBD colon, and normal tissues represented by colon, lung, thymus and kidney.


Panel 5D Summary: Ag2654/Ag2726 Highest expression of this gene was seen in adipose (CTs=30). This gene showed ubiquitous expression in this panel, with moderate expression in adipose, skeletal muscle, small intesting, uterus, placenta, kidney, and mesenchmyal stem cells.


F. CG50426-15 and CG50426-17 and CG50426-21: NEURESTIN


Expression of full-length physical clone CG50426-15 and variants CG50426-17 and CG50426-21 was assessed using the primer-probe sets Ag2679, Ag2728, Ag2975, Ag332, Ag047, and Ag47b, described in Tables FA, FB, FC, FD, FE, and FF. Results of the RTQ-PCR runs are shown in Tables FG, FH, Fl and FJ. The CG50426-15 variant only corresponds to Ag332 and Ag47b.

TABLE FAProbe Name Ag2679PrimersSequencesLengthStart PositionSEQ ID NoForward5′-ttgacctcaggaacggtttac-3′2111361240ProbeTET-5′-ctgctgcccaggaatactttctccag-3′-TAMRA2611721241Reverse5′-agtatttggagggcttcttcag-3′2212111242









TABLE FB










Probe Name Ag2728











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-ggagcttggtctcatgaccta-3′
21
4810
1243






Probe
TET-5′-actgggctcctggccaccaagag-3′-TAMRA
23
4841
1244





Reverse
5′-agtcgtccatcctgtttcatc-3′
21
4865
1245
















TABLE FC










Probe Name Ag2975











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-ggagcttggtctcatgaccta-3′
21
4810
1246






Probe
TET-5′-actgggctcctggccaccaagag-3′-TAMRA
23
4841
1247





Reverse
5′-agtcgtccatcctgtttcatc-3′
21
4865
1248
















TABLE FD










Probe Name Ag332











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-gctgccctgacttgtgcaa-3′
19
2211
1249






Probe
TET-5′-tctgacccagtgtgcatctcccgtt-3′-TAMRA
25
2234
1250





Reverse
5′-ccggtctggcagacacact-3′
19
2268
1251
















TABLE FE










Probe Name Ag047











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-ccaatgacctggccacca-3′
18
987
1252






Probe
TET-5′-ccagagtccgttcagcttcaggacagc-3′-TAMRA
27
1007
1253





Reverse
5′-gtggcacgttgctgtttagc-3′
20
1039
1254
















TABLE FF










Probe Name Ag47b











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-gaacgccggagcatacaga-3′
19
1574
1255






Probe
TET-5′-ccaggtactgcacaaacacggcttcat-3′-TAMRA
27
1605
1256





Reverse
5′-gatgccacaggcccaca-3′
17
1633
1257
















TABLE FG










Panel 1












Tissue Name
A
B
C
D
E















Endothelial cells
0.0
0.0
0.0
0.0
0.0


Endothelial cells (treated)
0.0
0.0
0.0
0.0
0.0


Pancreas
0.0
0.3
0.0
0.0
0.0


Pancreatic ca. CAPAN 2
0.0
0.0
0.0
0.0
0.0


Adrenal gland
0.0
1.3
0.2
0.0
0.0


Thyroid
0.0
0.4
100.0
0.1
0.0


Salivary gland
0.0
0.2
0.0
0.0
0.0


Pituitary gland
0.0
0.1
2.1
0.0
0.0


Brain (fetal)
0.0
15.0
3.4
11.0
21.8


Brain (whole)
95.9
67.8
3.2
4.5
32.5


Brain (amygdala)
0.0
8.8
3.8
8.7
12.2


Brain (cerebellum)
0.0
22.2
1.4
0.1
14.3


Brain (hippocampus)
0.0
24.7
3.4
8.7
15.6


Brain (substantia nigra)
3.4
3.6
1.3
2.3
3.8


Brain (thalamus)
3.5
4.7
3.3
5.0
9.1


Brain (hypothalamus)
0.0
0.7
0.2
0.0
0.0


Spinal cord
0.7
1.5
0.9
1.4
1.1


glio/astro U87-MG
0.6
2.6
1.6
1.7
3.0


glio/astro U-118-MG
0.0
0.6
0.2
1.1
0.1


astrocytoma SW1783
33.7
29.5
9.0
42.9
36.1


neuro*; met SK-N-AS
0.0
0.0
0.0
0.0
0.0


astrocytoma SF-539
31.0
36.9
10.3
68.3
48.3


astrocytoma SNB-75
33.7
32.8
7.1
23.0
50.3


glioma SNB-19
100.0
100.0
30.1
100.0
100.0


glioma U251
49.0
44.1
16.5
57.8
41.5


glioma SF-295
6.0
8.1
2.1
19.8
8.7


Heart
61.1
26.8
36.6
70.7
39.8


Skeletal muscle
0.0
0.1
0.2
0.0
0.0


Bone marrow
0.0
0.1
0.0
0.0
0.0


Thymus
18.7
18.4
1.1
2.7
17.2


Spleen
0.0
0.0
0.0
0.0
0.0


Lymph node
0.0
0.2
0.0
0.0
0.0


Colon (ascending)
0.5
0.7
0.7
0.9
4.5


Stomach
0.1
1.1
0.2
0.2
0.2


Small intestine
0.0
0.1
0.0
0.0
0.0


Colon ca. SW480
0.6
1.0
1.6
7.0
1.4


Colon ca.* SW620 (SW480 met)
0.0
0.0
0.0
0.0
0.0


Colon ca. HT29
0.0
0.0
0.0
0.0
0.0


Colon ca. HCT-116
0.0
0.0
0.0
0.0
0.0


Colon ca. CaCo-2
0.0
0.1
0.0
0.0
0.0


Colon ca. HCT-15
0.0
0.1
0.2
0.0
0.0


Colon ca. HCC-2998
0.0
0.0
0.0
0.0
0.0


Gastric ca. * (liver met) NCI-N87
0.0
0.4
0.2
0.0
0.0


Bladder
0.3
1.0
0.2
0.0
0.1


Trachea
0.0
0.4
0.2
0.0
0.0


Kidney
0.2
0.9
0.7
1.2
1.0


Kidney (fetal)
1.3
3.9
0.8
3.2
2.7


Renal ca. 786-0
10.6
11.7
3.4
7.9
12.2


Renal ca. A498
0.0
0.1
0.0
0.0
0.0


Renal ca. RXF 393
17.9
14.0
4.1
16.6
15.2


Renal ca. ACHN
0.0
0.0
0.0
0.0
0.0


Renal ca. UO-31
0.0
0.2
0.1
0.0
0.2


Renal ca. TK-10
0.0
0.0
0.0
0.0
0.0


Liver
0.0
3.2
0.8
0.9
1.9


Liver (fetal)
0.0
0.1
0.0
0.0
0.0


Liver ca. (hepatoblast) HepG2
0.0
0.0
0.0
0.0
0.0


Lung
0.0
0.2
0.0
0.0
0.0


Lung (fetal)
0.0
0.5
0.0
0.0
0.0


Lung ca. (small cell) LX-1
0.0
0.0
0.0
0.0
0.0


Lung ca. (small cell) NCI-H69
2.0
3.3
1.4
5.4
2.9


Lung ca. (s. cell var.) SHP-77
0.0
0.0
0.0
0.0
0.0


Lung ca. (large cell)NCI-H460
0.0
0.0
1.3
0.7
0.3


Lung ca. (non-sm. cell) A549
0.0
0.0
0.1
0.0
0.0


Lung ca. (non-s. cell) NCI-H23
0.0
0.0
0.0
0.0
0.0


Lung ca. (non-s. cell) HOP-62
5.2
4.7
3.9
11.3
6.7


Lung ca. (non-s. cl) NCI-H522
1.7
3.1
1.5
2.8
4.8


Lung ca. (squam.) SW 900
0.0
0.0
0.0
0.0
0.0


Lung ca. (squam.) NCI-H596
1.3
2.5
2.0
4.2
3.0


Mammary gland
11.9
9.1
4.6
7.0
10.6


Breast ca.* (pl. ef) MCF-7
0.0
0.0
0.0
0.0
0.0


Breast ca.* (pl. ef) MDA-MB-231
0.0
0.1
0.0
0.0
0.0


Breast ca.* (pl. ef) T47D
11.8
7.5
7.9
31.6
31.4


Breast ca. BT-549
0.0
0.0
4.5
13.8
28.3


Breast ca. MDA-N
0.0
0.1
0.0
0.0
0.0


Ovary
0.1
0.6
0.4
0.1
0.2


Ovarian ca. OVCAR-3
0.0
0.0
0.0
0.0
0.0


Ovarian ca. OVCAR-4
0.0
0.1
1.3
4.4
0.0


Ovarian ca. OVCAR-5
73.2
38.2
14.4
57.0
36.1


Ovarian ca. OVCAR-8
0.0
0.7
1.0
3.4
0.2


Ovarian ca. IGROV-1
0.0
0.0
0.8
2.6
0.0


Ovarian ca. (ascites) SK-OV-3
0.0
0.0
0.0
0.0
0.0


Uterus
0.4
1.2
0.6
1.7
2.9


Placenta
0.0
0.1
0.0
0.0
0.0


Prostate
0.9
1.9
0.7
1.9
1.7


Prostate ca.* (bone met) PC-3
0.0
0.0
0.1
0.0
0.0


Testis
25.7
22.2
0.7
0.7
26.4


Melanoma Hs688(A).T
23.8
21.6
5.0
20.0
28.9


Melanoma* (met) Hs688(B).T
4.6
6.5
3.4
9.6
9.2


Melanoma UACC-62
0.0
0.0
0.0
0.0
0.0


Melanoma M14
0.0
0.1
0.0
0.0
0.0


Melanoma LOX IMVI
3.7
3.4
0.6
0.2
2.7


Melanoma* (met) SK-MEL-5
0.0
0.0
0.0
0.0
0.0


Melanoma SK-MEL-28
0.0
0.0
3.6
17.4
0.0







Column A - Rel. Exp. (%) Ag047, Run 87354354





Column B - Rel. Exp. (%) Ag047, Run 87354779





Column C - Rel. Exp. (%) Ag332, Run 97803603





Column D - Rel. Exp. (%) Ag332, Run 98747043





Column E - Rel. Exp. (%) Ag47b, Run 88164379














TABLE FH










Panel 2D











Tissue Name
A
B
C
D














Normal Colon
5.5
8.9
7.4
10.4


CC Well to Mod Diff (ODO3866)
1.4
0.2
0.2
1.3


CC Margin (ODO3866)
0.4
0.5
0.4
0.1


CC Gr.2 rectosigmoid (ODO3868)
0.4
0.1
1.7
0.2


CC Margin (ODO3868)
2.5
0.9
0.7
0.7


CC Mod Diff (ODO3920)
0.0
0.0
0.0
0.2


CC Margin (ODO3920)
0.7
0.9
1.1
0.3


CC Gr.2 ascend colon (ODO3921)
0.0
0.7
0.6
0.2


CC Margin (ODO3921)
0.2
0.2
0.6
0.8


CC from Partial Hepatectomy
0.6
0.4
1.1
1.0


(ODO4309) Mets


Liver Margin (ODO4309)
11.1
13.6
30.4
21.2


Colon mets to lung (OD04451-01)
0.0
0.4
0.1
0.2


Lung Margin (OD04451-02)
1.3
1.0
0.5
0.7


Normal Prostate 6546-1
19.1
0.7
1.0
2.3


Prostate Cancer (OD04410)
5.8
2.9
4.7
3.4


Prostate Margin (OD04410)
4.7
5.7
7.2
4.9


Prostate Cancer (OD04720-01)
2.9
3.3
3.4
3.3


Prostate Margin (OD04720-02)
12.5
10.7
8.3
15.2


Normal Lung 061010
1.5
2.7
6.8
5.4


Lung Met to Muscle (ODO4286)
0.3
0.1
0.0
0.5


Muscle Margin (ODO4286)
0.3
0.2
0.3
0.6


Lung Malignant Cancer (OD03126)
0.4
0.2
0.8
1.5


Lung Margin (OD03126)
0.4
1.0
1.2
0.5


Lung Cancer (OD04404)
86.5
100.0
100.0
100.0


Lung Margin (OD04404)
18.3
3.3
2.2
3.0


Lung Cancer (OD04565)
100.0
52.1
62.0
77.9


Lung Margin (OD04565)
0.2
0.1
0.0
0.6


Lung Cancer (OD04237-01)
6.3
1.5
3.2
3.3


Lung Margin (OD04237-02)
1.4
0.5
0.5
0.6


Ocular Mel Met to Liver (ODO4310)
0.4
0.3
0.5
0.7


Liver Margin (ODO4310)
2.3
1.8
3.5
2.8


Melanoma Mets to Lung (OD04321)
0.0
0.3
0.4
1.6


Lung Margin (OD04321)
2.1
2.6
2.2
3.0


Normal Kidney
6.7
4.9
8.4
7.0


Kidney Ca, Nuclear grade 2
0.0
0.0
0.3
1.4


(OD04338)


Kidney Margin (OD04338)
3.5
1.5
4.1
3.5


Kidney Ca Nuclear grade 1/2
0.0
0.1
0.3
0.7


(OD04339)


Kidney Margin (OD04339)
18.4
10.3
8.4
15.5


Kidney Ca, Clear cell type
0.0
0.8
0.7
1.1


(OD04340)


Kidney Margin (OD04340)
6.5
4.4
4.5
6.5


Kidney Ca, Nuclear grade 3
90.8
36.1
54.3
50.3


(OD04348)


Kidney Margin (OD04348)
4.6
3.2
3.8
4.0


Kidney Cancer (OD04622-01)
2.7
1.8
4.1
3.5


Kidney Margin (OD04622-03)
2.0
0.2
0.3
1.1


Kidney Cancer (OD04450-01)
0.0
0.0
0.0
0.0


Kidney Margin (OD04450-03)
3.1
1.4
6.9
5.4


Kidney Cancer 8120607
1.5
0.3
0.5
1.8


Kidney Margin 8120608
3.4
0.8
1.3
2.2


Kidney Cancer 8120613
0.4
0.8
4.3
2.0


Kidney Margin 8120614
2.8
1.2
4.9
4.0


Kidney Cancer 9010320
76.8
39.0
36.3
52.9


Kidney Margin 9010321
10.4
5.1
4.9
4.3


Normal Uterus
0.0
0.2
0.3
0.0


Uterus Cancer 064011
0.9
0.0
0.3
1.9


Normal Thyroid
0.3
0.0
0.9
2.2


Thyroid Cancer 064010
0.0
0.0
0.0
0.0


Thyroid Cancer A302152
1.4
0.1
0.4
0.0


Thyroid Margin A302153
1.7
0.9
6.4
4.1


Normal Breast
20.3
5.7
13.0
20.9


Breast Cancer (OD04566)
0.3
0.1
0.5
0.3


Breast Cancer (OD04590-01)
2.4
2.2
1.1
1.9


Breast Cancer Mets (OD04590-03)
0.7
0.1
0.3
0.0


Breast Cancer Metastasis
6.9
3.3
7.7
9.3


(OD04655-05)


Breast Cancer 064006
5.6
8.3
4.2
4.9


Breast Cancer 1024
47.0
19.3
30.1
23.5


Breast Cancer 9100266
14.9
9.3
15.7
21.8


Breast Margin 9100265
4.6
1.5
4.4
8.4


Breast Cancer A209073
48.3
12.9
28.3
40.9


Breast Margin A209073
38.7
16.6
27.5
29.7


Normal Liver
0.2
0.1
0.0
0.2


Liver Cancer 064003
11.8
5.6
4.6
4.5


Liver Cancer 1025
4.5
1.6
4.2
2.8


Liver Cancer 1026
6.2
6.7
9.0
6.4


Liver Cancer 6004-T
15.6
3.3
3.5
2.8


Liver Tissue 6004-N
0.1
0.2
0.2
0.4


Liver Cancer 6005-T
14.6
8.0
8.7
7.9


Liver Tissue 6005-N
6.4
7.0
5.9
3.2


Normal Bladder
1.3
0.9
1.6
0.9


Bladder Cancer 1023
0.4
0.2
0.3
0.3


Bladder Cancer A302173
7.5
5.3
12.1
19.8


Bladder Cancer (OD04718-01)
27.7
23.0
35.6
41.5


Bladder Normal Adjacent
0.4
0.3
0.6
0.7


(OD04718-03)


Normal Ovary
1.4
0.4
1.1
0.3


Ovarian Cancer 064008
1.3
0.5
1.3
1.3


Ovarian Cancer (OD04768-07)
0.4
0.2
0.0
0.0


Ovary Margin (OD04768-08)
1.0
0.8
0.6
1.6


Normal Stomach
4.3
2.0
0.3
3.1


Gastric Cancer 9060358
2.4
0.7
0.7
1.2


Stomach Margin 9060359
0.0
0.0
0.7
4.3


Gastric Cancer 9060395
0.9
2.0
1.9
2.4


Stomach Margin 9060394
0.2
0.3
1.1
0.2


Gastric Cancer 9060397
0.4
0.5
1.6
0.7


Stomach Margin 9060396
0.0
0.1
0.2
0.2


Gastric Cancer 064005
0.7
0.7
0.9
1.5







Column A - Rel. Exp. (%) Ag047, Run 144771648





Column B - Rel. Exp. (%) Ag047, Run 152940364





Column C - Rel. Exp. (%) Ag2679, Run 158633803





Column D - Rel. Exp. (%) Ag2728, Run 158561830














TABLE FI










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
0.9



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
0.0



SNB-78- Glioma
100.0



SF-268- Glioblastoma
79.0



T98G- Glioblastoma
1.4



SK-N-SH- Neuroblastoma (metastasis)
2.0



SF-295- Glioblastoma
0.1



Cerebellum
4.5



Cerebellum
2.9



NCI-H292- Mucoepidermoid lung carcinoma
3.8



DMS-114- Small cell lung cancer
0.0



DMS-79- Small cell lung cancer
0.1



NCI-H146- Small cell lung cancer
0.0



NCI-H526- Small cell lung cancer
1.0



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
7.6



NCI-H157- Squamous cell lung cancer (metastasis)
0.8



NCI-H1155- Large cell lung cancer
0.0



NCI-H1299- Large cell lung cancer
0.2



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
0.0



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
0.0



KM12- Colon cancer
0.0



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
0.7



KATO III- Gastric carcinoma
0.0



NCI-SNU-16- Gastric carcinoma
0.2



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.3



RF-48- Gastric adenocarcinoma
0.1



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
0.0



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
4.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma (metastasis)
0.3



ES-2- Ovarian clear cell carcinoma
6.7



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
0.0



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
0.0



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
0.1



Caki-2- Clear cell renal carcinoma
0.1



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
6.0



Hs766T- Pancreatic carcinoma (LN metastasis)
0.0



CAPAN-1- Pancreatic adenocarcinoma (liver metastasis)
0.0



SU86.86- Pancreatic carcinoma (liver metastasis)
0.1



BxPC-3- Pancreatic adenocarcinoma
0.6



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.0



CFPAC-1- Pancreatic ductal adenocarcinoma
0.1



PANC-1- Pancreatic epithelioid ductal carcinoma
0.0



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
3.8



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
10.3



A204- Rhabdomyosarcoma
0.0



HT-1080- Fibrosarcoma
59.9



MG-63- Osteosarcoma
0.5



SK-LMS-1- Leiomyosarcoma (vulva)
4.2



SJRH30- Rhabdomyosarcoma (met to bone marrow)
0.3



A431- Epidermoid carcinoma
0.6



WM266-4- Melanoma
0.0



DU 145- Prostate carcinoma (brain metastasis)
0.0



MDA-MB-468- Breast adenocarcinoma
0.0



SCC-4- Squamous cell carcinoma of tongue
0.2



SCC-9- Squamous cell carcinoma of tongue
1.1



SCC-15- Squamous cell carcinoma of tongue
0.3



CAL 27- Squamous cell carcinoma of tongue
7.0









Column A - Rel. Exp. (%) Ag047, Run 158634002














TABLE FJ










Panel 4.1D










Tissue Name
A
B
C













Secondary Th1 act
0.0
0.0
0.0


Secondary Th2 act
0.0
0.0
0.1


Secondary Tr1 act
0.0
0.0
0.0


Secondary Th1 rest
0.0
0.0
0.0


Secondary Th2 rest
0.0
0.0
0.2


Secondary Tr1 rest
0.0
0.0
0.0


Primary Th1 act
0.0
0.0
0.1


Primary Th2 act
0.0
0.0
0.0


Primary Tr1 act
0.0
0.0
0.0


Primary Th1 rest
0.0
0.0
0.0


Primary Th2 rest
0.0
0.0
0.0


Primary Tr1 rest
0.0
0.0
0.0


CD45RA CD4 lymphocyte act
0.6
0.8
1.7


CD45RO CD4 lymphocyte act
0.2
0.0
0.2


CD8 lymphocyte act
0.0
0.0
0.0


Secondary CD8 lymphocyte rest
0.1
0.1
0.0


Secondary CD8 lymphocyte act
0.0
0.0
0.0


CD4 lymphocyte none
0.0
0.0
0.2


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0
0.0


LAK cells rest
0.0
0.0
0.0


LAK cells IL-2
0.0
0.0
0.1


LAK cells IL-2 + IL-12
0.0
0.0
0.0


LAK cells IL-2 + IFN gamma
0.0
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.1
0.2


LAK cells PMA/ionomycin
0.0
0.0
0.0


NK Cells IL-2 rest
0.0
0.0
0.0


Two Way MLR 3 day
0.0
0.0
0.0


Two Way MLR 5 day
0.0
0.0
0.1


Two Way MLR 7 day
0.0
0.1
0.0


PBMC rest
0.0
0.0
0.0


PBMC PWM
0.0
0.0
0.0


PBMC PHA-L
0.0
0.0
0.0


Ramos (B cell) none
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0


B lymphocytes PWM
0.0
0.0
0.0


B lymphocytes CD40L and IL-4
0.0
0.0
0.0


EOL-1 dbcAMP
0.0
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0


Dendritic cells none
0.0
0.0
0.0


Dendritic cells LPS
0.0
0.0
0.0


Dendritic cells anti-CD40
0.0
0.1
0.3


Monocytes rest
0.0
0.0
0.0


Monocytes LPS
0.0
0.0
0.2


Macrophages rest
0.0
0.0
0.0


Macrophages LPS
0.0
0.0
0.0


HUVEC none
0.0
0.0
0.3


HUVEC starved
0.0
0.4
0.2


HUVEC IL-1beta
0.0
0.0
0.0


HUVEC IFN gamma
0.0
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0
0.2


HUVEC TNF alpha + IL4
0.0
0.0
0.0


HUVEC IL-11
0.0
0.0
0.2


Lung Microvascular EC none
0.0
0.0
0.0


Lung Microvascular EC TNFalpha +
0.0
1.1
0.0


IL-1beta


Microvascular Dermal EC none
0.0
0.0
0.0


Microsvasular Dermal EC
0.0
0.0
0.0


TNFalpha + IL-1beta


Bronchial epithelium TNFalpha +
17.3
37.4
28.5


IL1beta


Small airway epithelium none
40.3
47.6
49.0


Small airway epithelium TNFalpha +
100.0
100.0
100.0


IL-1beta


Coronery artery SMC rest
0.7
0.8
0.4


Coronery artery SMC TNFalpha + IL-
0.5
0.6
1.3


1beta


Astrocytes rest
26.1
51.8
24.5


Astrocytes TNFalpha + IL-1 beta
24.1
78.5
42.6


KU-812 (Basophil) rest
0.0
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0
0.0


CCD1106 (Keratinocytes) none
52.5
45.1
90.1


CCD1106 (Keratinocytes)
14.4
33.7
20.9


TNFalpha + IL-1 beta


Liver cirrhosis
0.3
0.2
0.2


NCI-H292 none
0.4
0.5
0.7


NCI-H292 IL-4
9.9
12.6
19.1


NCI-H292 IL-9
0.8
0.6
1.6


NCI-H292 IL-13
14.0
10.9
13.2


NCI-H292 IFN gamma
0.1
0.9
0.4


HPAEC none
0.0
1.2
0.4


HPAEC TNF alpha + IL-1 beta
0.0
0.3
0.0


Lung fibroblast none
0.7
1.5
1.6


Lung fibroblast TNF alpha + IL-1
0.2
0.0
0.7


beta


Lung fibroblast IL-4
0.1
0.1
0.0


Lung fibroblast IL-9
0.2
0.2
0.1


Lung fibroblast IL-13
0.2
1.3
0.4


Lung fibroblast IFN gamma
0.0
0.8
0.0


Dermal fibroblast CCD1070 rest
4.8
4.1
5.7


Dermal fibroblast CCD1070 TNF
1.3
2.2
2.1


alpha


Dermal fibroblast CCD1070 IL-1 beta
1.1
0.8
1.8


Dermal fibroblast IFN gamma
3.1
2.4
3.4


Dermal fibroblast IL-4
3.9
5.4
6.0


Dermal Fibroblasts rest
7.4
9.3
9.3


Neutrophils TNFa + LPS
0.0
0.6
0.0


Neutrophils rest
0.0
0.0
0.0


Colon
0.0
0.5
0.0


Lung
0.1
1.6
0.1


Thymus
1.6
9.3
2.3


Kidney
1.5
11.3
1.7







Column A - Rel. Exp. (%) Ag047, Run 264524965





Column B - Rel. Exp. (%) Ag2975, Run 171818669





Column C - Rel. Exp. (%) Ag47b, Run 263594820







Panel 1 Summary: Ag047/Ag332/Ag47b Highest expression of this gene was detected in a brain cancer cell line (CTs=23-25). There was also significant expression in clusters of samples from melanoma, ovarian cancer, breast, lung, renal colon and brain cancer lines. Expression of this gene is associated with these forms of cancer and is useful as a diagnostic marker for the presence of these cancers. Therapeutic modulation of the expression or function of these genes or gene products is useful in the treatment of ovarian, breast, lung, renal, and brain cancer and melanoma.


In addition to significant expression in brain cancer cell lines, this gene was preferentially expressed in the brain. This expression profile shows that this gene product plays a role in CNS processes. This gene encodes a homolog of a member of the neurestin farnily, Ten M2, and plays a role in neuronal regeneration. (Otaki, J. Dev Biol 1999 August 1;212(1):165-81 ) Agents that induce the expression or activity of this gene or gene product have utility as neuronal regeneration drugs and specifically for the treatment of neurodegenerative diseases, stroke, and neuronal trauma.


Among tissues with metabolic function, this gene showed consistent expression in thyroid, adult and fetal heart, liver and skeletal muscle. Thus, this gene product is an antibody target for the treatment of metabolic and endocrine disease, including obesity and Types 1 and 2 diabetes. In addition, this gene was eressed at higher levels in adult liver than in fetal liver and is be useful for differentiating between the two sources of liver tissue.


Panel 2D Summary: Ag047/Ag2679/Ag2728 Highest expression of this gene was detected in lung cancer samples (CTs=27-29). This gene was expressed at a higher level in gastric, bladder, and two samples each of lung and kidney cancer relative to the normal adjacent tissues. Expression of this gene is useful as a marker to detect the presence of these cancers. Therapeutic modulation of this gene, expressed protein and/or use of antibodies, protein therapeutics or small molecule drugs are useful in the treatment of these cancers.


Panel 3D Summary: Ag047 This gene was expressed in squamous cell carcinoma, glioma, small cell lung cancer cell lines. Expression of this gene is associated with these cancers and therapeutic modulation of this gene expressed protein and/or use of antibodies, protein therapeutics or small molecule drugs is of use in the treatment of these cancers.


Panel 4.1D Summary: Ag047/Ag047b/Ag2975 Highest expression of this gene was detected in activated small airway epithelium (CT=27-32). This gene was expressed at moderate level in small airway epithelium, astrocytes and keratinocytes. The expression of this gene in these tissues was up-regulated upon treatment with the inflammatory cytokines TNF-a and IL1. This gene encodes for a neurestin like molecule whose role in neuronal regeneration has been demonstrated. Modulation of the expression of this gene or its expressed protein is useful in the regeneration or repair mechanism of these tissues during inflammation and for the treatment of inflammatory brain and lung diseases such as bronchitis, chronic pulmonary diseases, skin and CNS inflammatory diseases, multiple sclerosis or stroke.


G. CG50646-04: Polydom Protein


Expression of gene CG50646-04 was assessed using the primer-probe sets Ag768 and Ag984, described in Tables GA and GB. Results of the RTQ-PCR runs are shown in Tables GC and GD.

TABLE GAProbe Name Ag768PrimersSequencesLengthStart PositionSEQ ID NoForward5′-gggctataagtcagtcggaagt-3′2267721258ProbeTET-5′-cctgtatttgtctgccaagccaatcg-3′-TAMRA2667941259Reverse5′-acagtcgagaggaacacacatc-3′2268441260









TABLE GB










Probe Name Ag984
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-cccgggttacactgatacct-3′
20
6755
1261






Probe
TET-5′-tgctccagaaagccattctcaacctt-3′-
26
6698
1262



TAMRA





Reverse
5′-cggtatcttgtggtgaacca-3′
20
6675
1263
















TABLE GC










Panel 1.2










Tissue Name
A














Endothelial cells
0.0



Heart (Fetal)
0.0



Pancreas
0.0



Pancreatic ca. CAPAN 2
0.0



Adrenal Gland
0.1



Thyroid
0.0



Salivary gland
0.0



Pituitary gland
0.0



Brain (fetal)
0.0



Brain (whole)
0.0



Brain (amygdala)
0.0



Brain (cerebellum)
0.0



Brain (hippocampus)
0.0



Brain (thalamus)
0.0



Cerebral Cortex
0.0



Spinal cord
0.0



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.0



glioma U251
0.0



glioma SF-295
0.0



Heart
0.1



Skeletal Muscle
0.2



Bone marrow
0.0



Thymus
0.0



Spleen
0.0



Lymph node
0.7



Colorectal Tissue
0.0



Stomach
0.0



Small intestine
0.8



Colon ca. SW480
0.0



Colon ca.* SW620 (SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. Tissue (ODO3866)
0.0



Colon ca. HCC-2998
0.0



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.0



Trachea
0.0



Kidney
0.0



Kidney (fetal)
0.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
0.6



Lung (fetal)
0.0



Lung ca. (small cell) LX-1
0.0



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
4.4



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
0.0



Breast ca. MDA-N
0.0



Ovary
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca. (ascites) SK-OV-3
0.0



Uterus
0.2



Placenta
100.0



Prostate
0.0



Prostate ca.* (bone met) PC-3
0.0



Testis
0.0



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0









Column A - Rel. Exp. (%) Ag768, Run 116422776














TABLE GD










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.1



Secondary Tr1 act
0.0



Secondary Th1 rest
0.1



Secondary Th2 rest
0.1



Secondary Tr1 rest
0.2



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.2



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
6.4



CD45RO CD4 lymphocyte act
0.1



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.1



Secondary CD8 lymphocyte act
0.1



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.2



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.1



LAK cells IL-2 + IFN gamma
0.3



LAK cells IL-2 + IL-18
0.2



LAK cells PMA/ionomycin
3.5



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
2.5



PBMC PHA-L
0.3



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.1



B lymphocytes PWM
0.1



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.2



Dendritic cells anti-CD40
0.1



Monocytes rest
0.1



Monocytes LPS
3.1



Macrophages rest
0.1



Macrophages LPS
3.3



HUVEC none
0.0



HUVEC starved
0.2



HUVEC IL-1beta
0.2



HUVEC IFN gamma
0.3



HUVEC TNF alpha + IFN gamma
0.1



HUVEC TNF alpha + IL4
0.1



HUVEC IL-11
0.1



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.1



Microsvasular Dermal EC TNFalpha + IL-1beta
0.2



Bronchial epithelium TNFalpha + IL1beta
0.2



Small airway epithelium none
2.3



Small airway epithelium TNFalpha + IL-1beta
1.3



Coronery artery SMC rest
15.1



Coronery artery SMC TNFalpha + IL-1beta
8.2



Astrocytes rest
1.8



Astrocytes TNFalpha + IL-1beta
2.4



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.4



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
1.4



Liver cirrhosis
10.6



Lupus kidney
7.0



NCI-H292 none
24.1



NCI-H292 IL-4
21.6



NCI-H292 IL-9
30.4



NCI-H292 IL-13
15.3



NCI-H292 IFN gamma
14.6



HPAEC none
0.4



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
31.0



Lung fibroblast TNF alpha + IL-1 beta
7.7



Lung fibroblast IL-4
55.5



Lung fibroblast IL-9
37.4



Lung fibroblast IL-13
86.5



Lung fibroblast IFN gamma
100.0



Dermal fibroblast CCD1070 rest
28.9



Dermal fibroblast CCD1070 TNF alpha
23.7



Dermal fibroblast CCD1070 IL-1 beta
20.2



Dermal fibroblast IFN gamma
22.5



Dermal fibroblast IL-4
47.0



IBD Colitis 2
0.5



IBD Crohn's
2.0



Colon
9.2



Lung
20.3



Thymus
13.0



Kidney
6.4









Column A - Rel. Exp. (%) Ag768, Run 138175130







Panel 1.2 Summary: Ag768 Highest expression of this gene was seen in placenta (CT=21). This gene encodes a polydom-like protein and was also highly expressed in mammary gland, skeletal muscle. This gene may be involved in cellular adhesion (Gilges D. Biochem J 2000 November 15;352 Pt 1:49-59). Expression of this gene is useful in differentiating between placental tissues and other tissues on this panel. Modulation of this gene or its protein product is useful in reproductive and skeletal muscle physiology.


This gene was more highly expressed in fetal kidney (CT=33) than in adult kidney (CT=40). Conversely, this gene was more highly expressed in adult lung and liver (CTs=28-32) than in fetal lung and liver (CTs=38-40). Thus, expression of this gene is useful in differentiating between the adult and fetal sources of these tissues.


Panel 4D Summary: Ag768 Highest expression of this gene was seen in lung fibroblasts stimulated with IFN-gamma (CT=27.4). Significant expression was seen in many samples derived from the lung including lung fibroblasts stimulated with different cytokines, the pulmonary mucoepidermoid cell line H292 stimulated with the same cytokines, and normal lung tissue. The expression of this gene in lung cells and lung tissue shows that this gene or its protein product is involved in normal homeostasis of the lung, as well as pathological and inflammatory lung disorders, including chronic obstructive pulmonary disease, asthma, allergy and emphysema.


Significant levels of expression of this gene in dermal fibroblasts show that this gene and its protein product is involved in skin disorders, including psoriasis.


Moderate to low expression of this gene was also seen in many other cells with important immune function, including stimulated macrophages and monocytes, coronary artery smooth muscle cells, stimulated peripheral blood mononuclear cells, lymphocyte activated killer cells (LAK), astrocytes, activated CD45RA cells, and normal colon, thymus and kidney. This widespread expression demonstrates that this gene or the protein encoded by this gene is involved in other inflammatory and autoimmune conditions, including inflammatory bowel disease, rheumatoid arthritis and osteoarthritis.


H. CG5073609: CD44-Like Precursor Fell


Expression of gene CG50736-09 was assessed using the primer-probe sets Ag2742, Ag2743, Ag2744, Ag2745, Ag2746, Ag793, Ag812, Ag03 and Ag068, described in Tables HA, HB, HC, HD, HE, HF, HG, HH and HI. Results of the RTQ-PCR runs are shown in Tables HJ, HK, HL, HM, HN and HO.

TABLE HAProbe Name Ag2742SEQStartIDPrimersSequencesLengthPositionNoForward5′-ctgcaaaatcttacgactttgg-3′224291264ProbeTET-5′-caacaaacaatggctacatcaaatttagca-3′-304511265TAMRAReverse5′-atgacactcagcaaacctgagt-3′224931266









TABLE HB










Probe Name Ag2743
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctgcaaaatcttacgacttgg-3′
22
429
1267






Probe
TET-5′-caacaaacaatggctacatcaaatttagca-3′-
30
451
1268



TAMRA





Reverse
5′-atgacactcagcaaacctgagt-3′
22
493
1269
















TABLE HC










Probe Name Ag2744
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctgcaaaatcttacgactttgg-3′
22
429
1270






Probe
TET-5′-caacaaacaatggctacatcaaatttagca-3′-
30
451
1271



TAMRA





Reverse
5′-tcagcaaacctgagtcctgta-3′
21
487
1272
















TABLE HD










Probe Name Ag2745
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctgcaaaatcttacgactttgg-3′
22
429
1273






Probe
TET-5′-caacaaacaatggctacatcaaatttagca-3′-
30
451
1274



TAMRA





Reverse
5′-atgacactcagcaaacctgagt-3′
22
493
1275
















TABLE HD










Probe Name Ag2746
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctgcaaaatcttacgactttgg-3′
22
429
1276






Probe
TET-5′-caacaaacaatggctacatcaaatttagca-3′-
30
451
1277



TAMRA





Reverse
5′-atgacactcagcaaacctgagt-3′
22
493
1278
















TABLE HF










Probe Name Ag793
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ccaaggttttagctgtggatct-3′
22
664
1279






Probe
TET-5′-acatccactgcctggaagaccctg-3′-
24
690
1280



TAMRA





Reverse
5′-cacatttcacactcagctctga-3′
22
720
1281
















TABLE HG










Probe Name Ag812
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-caggagcatttcgtgaaaga-3′
20
57
1282






Probe
TET-5′-ttttgcacctttatctgcagcctttg-3′-
26
104
1283



TAMRA





Reverse
5′-tttaacccgagcttcctcat-3′
20
130
1284
















TABLE HH










Probe Name Ag03
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctggttgtaggttgccatggt-3′
21
1884
1285






Probe
TET-5′-cagcttcgttggcacaggcctctc-3′-
24
1858
1286



TAMRA





Reverse
5′-ccagtataagctgacctttgacaaag-3′
26
1829
1287
















TABLE HI










Probe Name Ag068
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctggttgtaggttgccatggt-3′
21
1884
1288






Probe
TET-5′-cagcttcgttggcacaggcctctc-3′-
24
1858
1289



TAMRA





Reverse
5′-ccagtataagctgacctttgacaaag-3′
26
1829
1290
















TABLE HJ










Panel 1











Tissue Name
A
B















Endothelial cells
0.0
0.0



Endothelial cells (treated)
0.0
0.0



Pancreas
0.0
2.3



Pancreatic ca. CAPAN 2
0.0
0.0



Adrenal gland
0.5
1.3



Thyroid
1.8
2.1



Salivary gland
2.5
3.0



Pituitary gland
0.8
0.8



Brain (fetal)
0.1
0.4



Brain (whole)
0.3
0.6



Brain (amygdala)
0.1
0.4



Brain (cerebellum)
0.4
0.7



Brain (hippocampus)
0.3
0.9



Brain (substantia nigra)
0.1
0.3



Brain (thalamus)
0.1
0.2



Brain (hypothalamus)
0.4
0.5



Spinal cord
0.0
0.3



glio/astro U87-MG
0.0
0.1



glio/astro U-118-MG
0.1
0.2



astrocytoma SW1783
0.0
0.0



neuro*; met SK-N-AS
0.0
0.0



astrocytoma SF-539
0.0
0.1



astrocytoma SNB-75
0.1
0.2



glioma SNB-19
0.6
1.6



glioma U251
0.2
0.9



glioma SF-295
0.1
0.2



Heart
0.3
0.3



Skeletal muscle
0.3
0.4



Bone marrow
3.5
3.0



Thymus
0.3
0.2



Spleen
100.0
100.0



Lymph node
29.3
81.2



Colon (ascending)
0.8
1.0



Stomach
1.2
1.5



Small intestine
1.6
1.7



Colon ca. SW480
0.0
0.0



Colon ca.* SW620 (SW480 met)
0.0
0.1



Colon ca. HT29
0.0
0.1



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
0.1
0.1



Colon ca. HCT-15
0.1
0.7



Colon ca. HCC-2998
0.0
0.3



Gastric ca. * (liver met) NCI-N87
0.1
0.2



Bladder
1.1
0.2



Trachea
2.4
2.2



Kidney
0.1
0.4



Kidney (fetal)
1.0
1.3



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.1
0.5



Renal ca. RXF 393
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.1



Renal ca. TK-10
0.2
0.9



Liver
14.2
12.9



Liver (fetal)
25.7
15.6



Liver ca. (hepatoblast) HepG2
0.0
0.1



Lung
0.5
0.1



Lung (fetal)
4.4
6.2



Lung ca. (small cell) LX-1
0.0
0.0



Lung ca. (small cell) NCI-H69
0.1
0.4



Lung ca. (s. cell var.) SHP-77
0.0
0.0



Lung ca. (large cell)NCI-H460
0.0
0.0



Lung ca. (non-sm. cell) A549
0.0
0.1



Lung ca. (non-s. cell) NCI-H23
0.0
0.3



Lung ca. (non-s. cell) HOP-62
0.0
0.1



Lung ca. (non-s. cl) NCI-H522
0.0
0.0



Lung ca. (squam.) SW 900
0.0
0.1



Lung ca. (squam.) NCI-H596
0.1
0.3



Mammary gland
4.7
5.1



Breast ca.* (pl. ef) MCF-7
0.0
0.2



Breast ca.* (pl. ef) MDA-MB-231
0.1
0.4



Breast ca.* (pl. ef) T47D
0.1
0.4



Breast ca. BT-549
0.0
0.0



Breast ca. MDA-N
0.1
0.6



Ovary
3.6
1.3



Ovarian ca. OVCAR-3
0.0
0.1



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
0.1
0.6



Ovarian ca. OVCAR-8
0.2
0.7



Ovarian ca. IGROV-1
0.0
0.3



Ovarian ca. (ascites) SK-OV-3
0.0
0.2



Uterus
0.2
0.4



Placenta
1.9
1.6



Prostate
0.7
1.0



Prostate ca.* (bone met) PC-3
0.0
0.0



Testis
23.5
22.1



Melanoma Hs688(A).T
0.0
0.1



Melanoma* (met) Hs688(B).T
0.0
0.1



Melanoma UACC-62
0.0
0.0



Melanoma M14
0.1
0.5



Melanoma LOX IMVI
0.1
0.5



Melanoma* (met) SK-MEL-5
0.0
0.0



Melanoma SK-MEL-28
0.3
1.3









Column A - Rel. Exp. (%) Ag03, Run 87353672






Column B - Rel. Exp. (%) Ag068, Run 87361479














TABLE HK










Panel 1.2











Tissue Name
A
B















Endothelial cells
0.0
0.0



Heart (Fetal)
0.3
5.6



Pancreas
2.0
0.6



Pancreatic ca. CAPAN 2
0.0
0.0



Adrenal Gland
0.3
0.9



Thyroid
2.0
0.7



Salivary gland
6.3
6.7



Pituitary gland
0.1
0.4



Brain (fetal)
0.1
0.0



Brain (whole)
1.2
0.1



Brain (amygdala)
0.0
0.1



Brain (cerebellum)
7.6
0.0



Brain (hippocampus)
0.1
0.2



Brain (thalamus)
0.0
0.0



Cerebral Cortex
0.1
0.1



Spinal cord
0.0
0.1



glio/astro U87-MG
0.0
0.0



glio/astro U-118-MG
0.0
0.0



astrocytoma SW1783
0.0
0.0



neuro*; met SK-N-AS
0.0
0.0



astrocytoma SF-539
0.0
0.1



astrocytoma SNB-75
0.0
0.0



glioma SNB-19
0.0
0.1



glioma U251
0.0
0.1



glioma SF-295
0.0
0.0



Heart
0.8
1.8



Skeletal Muscle
2.4
1.5



Bone marrow
2.7
3.5



Thymus
0.2
0.3



Spleen
44.8
44.4



Lymph node
39.2
51.8



Colorectal Tissue
0.0
0.2



Stomach
1.0
2.9



Small intestine
1.2
2.7



Colon ca. SW480
0.0
0.0



Colon ca.* SW620 (SW480 met)
0.0
0.0



Colon ca. HT29
0.0
0.1



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
0.0
0.0



Colon ca. Tissue (ODO3866)
0.1
0.4



Colon ca. HCC-2998
0.0
0.0



Gastric ca.* (liver met) NCI-N87
0.0
0.1



Bladder
3.7
3.8



Trachea
1.1
1.9



Kidney
0.1
0.4



Kidney (fetal)
0.8
2.1



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.3
0.1



Renal ca. RXF 393
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.0
0.0



Liver
100.0
100.0



Liver (fetal)
37.9
58.2



Liver ca. (hepatoblast) HepG2
0.0
0.0



Lung
0.4
0.8



Lung (fetal)
2.4
2.5



Lung ca. (small cell) LX-1
0.0
0.0



Lung ca. (small cell) NCI-H69
0.1
0.6



Lung ca. (s. cell var.) SHP-77
0.0
0.0



Lung ca. (large cell) NCI-H460
0.0
0.2



Lung ca. (non-sm. cell) A549
0.1
0.2



Lung ca. (non-s. cell) NCI-H23
0.0
0.1



Lung ca. (non-s. cell) HOP-62
0.0
0.0



Lung ca. (non-s. cl) NCI-H522
0.0
0.0



Lung ca. (squam.) SW 900
0.0
0.0



Lung ca. (squam.) NCI-H596
0.1
0.2



Mammary gland
2.9
2.3



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0



Breast ca.* (pl. ef) T47D
0.1
0.3



Breast ca. BT-549
0.0
0.0



Breast ca. MDA-N
0.0
0.1



Ovary
1.4
4.2



Ovarian ca. OVCAR-3
2.9
0.0



Ovarian ca. OVCAR-4
4.1
0.0



Ovarian ca. OVCAR-5
0.2
0.3



Ovarian ca. OVCAR-8
0.0
0.1



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca. (ascites) SK-OV-3
0.0
0.0



Uterus
0.2
0.7



Placenta
0.8
0.9



Prostate
0.3
0.5



Prostate ca.* (bone met) PC-3
0.0
0.0



Testis
12.2
8.4



Melanoma Hs688(A).T
0.0
0.0



Melanoma* (met) Hs688(B).T
0.0
0.1



Melanoma UACC-62
0.0
0.0



Melanoma M14
0.1
0.2



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.1
0.0









Column A - Rel. Exp. (%) Ag812, Run 118348259






Column B - Rel. Exp. (%) Ag812, Run 121953945














TABLE HL










Panel 1.3D












Tissue Name
A
B
C
D
E















Liver adenocarcinoma
0.0
0.0
0.0
0.0
0.0


Pancreas
0.2
0.4
0.3
0.2
0.2


Pancreatic ca. CAPAN 2
0.0
0.0
0.0
0.0
0.0


Adrenal gland
0.2
0.0
0.0
0.4
0.2


Thyroid
0.5
0.6
1.1
1.4
0.6


Salivary gland
1.0
1.0
0.7
0.7
0.1


Pituitary gland
0.0
0.0
0.1
0.0
0.0


Brain (fetal)
0.0
0.0
0.0
0.0
0.0


Brain (whole)
0.0
0.0
0.1
0.0
0.0


Brain (amygdala)
0.0
0.3
0.1
0.0
0.0


Brain (cerebellum)
0.0
0.0
0.0
0.0
0.0


Brain (hippocampus)
0.3
0.0
0.1
0.0
0.0


Brain (substantia nigra)
0.0
0.0
0.0
0.0
0.0


Brain (thalamus)
0.0
0.0
0.0
0.0
0.0


Cerebral Cortex
0.0
0.0
0.0
0.1
0.0


Spinal cord
0.0
0.0
0.0
0.0
0.0


glio/astro U87-MG
0.0
0.0
0.0
0.0
0.0


glio/astro U-118-MG
0.0
0.0
0.0
0.0
0.0


astrocytoma SW1783
0.0
0.0
0.0
0.0
0.0


neuro*; met SK-N-AS
0.0
0.0
0.0
0.0
0.0


astrocytoma SF-539
0.0
0.0
0.0
0.0
0.0


astrocytoma SNB-75
0.0
0.0
0.0
0.0
0.0


glioma SNB-19
0.0
0.0
0.0
0.0
0.0


glioma U251
0.0
0.0
0.0
0.0
0.0


glioma SF-295
0.0
0.0
0.0
0.0
0.0


Heart (fetal)
1.8
0.7
2.1
2.1
1.5


Heart
0.1
0.2
0.0
0.1
0.2


Skeletal muscle (fetal)
8.5
6.9
9.3
9.5
6.8


Skeletal muscle
0.0
0.0
0.0
0.1
0.0


Bone marrow
2.6
2.2
3.3
2.4
3.1


Thymus
0.1
0.0
0.1
0.1
0.2


Spleen
100.0
100.0
100.0
100.0
100.0


Lymph node
20.7
17.9
25.5
26.4
32.8


Colorectal
1.6
1.1
0.6
0.7
0.4


Stomach
1.3
0.5
1.2
0.7
0.9


Small intestine
1.3
1.0
1.1
1.1
1.2


Colon ca. SW480
0.0
0.0
0.0
0.0
0.0


Colon ca.* SW620(SW480 met)
0.0
0.0
0.0
0.0
0.0


Colon ca. HT29
0.0
0.0
0.0
0.0
0.0


Colon ca. HCT-116
0.0
0.0
0.0
0.0
0.0


Colon ca. CaCo-2
0.0
0.0
0.0
0.0
0.0


Colon ca. tissue(ODO3866)
0.1
0.1
0.0
0.2
0.1


Colon ca. HCC-2998
0.0
0.0
0.0
0.0
0.0


Gastric ca.* (liver met) NCI-N87
0.0
0.0
0.0
0.0
0.1


Bladder
0.3
0.4
0.6
0.6
0.4


Trachea
1.3
0.7
1.4
1.2
1.3


Kidney
0.0
0.0
0.1
0.0
0.0


Kidney (fetal)
2.4
3.4
3.8
2.1
3.0


Renal ca. 786-0
0.0
0.0
0.0
0.0
0.0


Renal ca. A498
0.0
0.0
0.0
0.0
0.0


Renal ca. RXF 393
0.0
0.0
0.0
0.0
0.0


Renal ca. ACHN
0.0
0.0
0.0
0.0
0.0


Renal ca. UO-31
0.0
0.0
0.0
0.0
0.0


Renal ca. TK-10
0.0
0.0
0.0
0.0
0.0


Liver
5.6
8.8
6.1
6.4
10.3


Liver (fetal)
33.4
33.2
33.9
33.4
36.6


Liver ca. (hepatoblast) HepG2
0.0
0.0
0.0
0.0
0.0


Lung
0.6
0.6
0.6
0.7
0.2


Lung (fetal)
2.0
1.9
2.4
1.0
3.1


Lung ca. (small cell) LX-1
0.0
0.0
0.0
0.0
0.0


Lung ca. (small cell) NCI-H69
0.0
0.0
0.0
0.0
0.0


Lung ca. (s. cell var.) SHP-77
0.0
0.0
0.0
0.0
0.0


Lung ca. (large cell)NCI-H460
0.0
0.0
0.0
0.0
0.0


Lung ca. (non-sm. cell) A549
0.0
0.0
0.0
0.0
0.0


Lung ca. (non-s. cell) NCI-H23
0.0
0.0
0.0
0.0
0.0


Lung ca. (non-s. cell) HOP-62
0.0
0.0
0.0
0.0
0.0


Lung ca. (non-s. cl) NCI-H522
0.0
0.0
0.0
0.0
0.0


Lung ca. (squam.) SW 900
0.0
0.0
0.0
0.0
0.0


Lung ca. (squam.) NCI-H596
0.0
0.0
0.0
0.0
0.0


Mammary gland
1.6
1.5
1.1
1.7
1.5


Breast ca.* (pl. ef) MCF-7
0.0
0.0
0.0
0.0
0.0


Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0
0.0
0.0
0.0


Breast ca.* (pl. ef) T47D
0.0
0.0
0.0
0.0
0.0


Breast ca. BT-549
0.0
0.0
0.0
0.0
0.0


Breast ca. MDA-N
0.0
0.0
0.0
0.0
0.0


Ovary
5.0
5.4
4.5
6.0
4.6


Ovarian ca. OVCAR-3
0.0
0.0
0.0
0.0
0.0


Ovarian ca. OVCAR-4
0.0
0.0
0.0
0.0
0.0


Ovarian ca. OVCAR-5
0.0
0.0
0.0
0.0
0.0


Ovarian ca. OVCAR-8
0.0
0.0
0.0
0.0
0.0


Ovarian ca. IGROV-1
0.0
0.0
0.0
0.0
0.0


Ovarian ca.* (ascites) SK-OV-3
0.0
0.0
0.0
0.0
0.0


Uterus
0.2
0.2
0.5
0.3
0.1


Placenta
0.4
0.0
0.4
0.1
0.2


Prostate
0.2
0.1
0.1
0.1
0.1


Prostate ca.* (bone met)PC-3
0.0
0.0
0.0
0.0
0.0


Testis
7.5
5.9
4.7
6.5
5.6


Melanoma Hs688(A).T
0.0
0.0
0.0
0.0
0.0


Melanoma* (met) Hs688(B).T
0.0
0.0
0.0
0.0
0.0


Melanoma UACC-62
0.0
0.0
0.0
0.0
0.0


Melanoma M14
0.0
0.0
0.0
0.0
0.0


Melanoma LOX IMVI
0.0
0.0
0.0
0.0
0.0


Melanoma* (met) SK-MEL-5
0.0
0.0
0.0
0.0
0.0


Adipose
1.0
1.5
0.6
0.5
1.1







Column A - Rel. Exp. (%) Ag2742, Run 153641674





Column B - Rel. Exp. (%) Ag2743, Run 153658349





Column C - Rel. Exp. (%) Ag2744, Run 153670718





Column D - Rel. Exp. (%) Ag2745, Run 153664738





Column E - Rel. Exp. (%) Ag2746, Run 153675151














TABLE HM










Panel 2D












Tissue Name
A
B
C
D
E















Normal Colon
3.2
3.8
4.4
4.8
4.5


CC Well to Mod Diff (ODO3866)
0.1
0.1
0.1
0.0
0.0


CC Margin (ODO3866)
0.7
0.5
0.9
1.2
0.3


CC Gr.2 rectosigmoid (ODO3868)
0.2
0.4
0.2
0.2
0.1


CC Margin (ODO3868)
0.1
0.1
0.0
0.2
0.1


CC Mod Diff (ODO3920)
0.0
0.1
0.1
0.0
0.0


CC Margin (ODO3920)
1.1
2.0
3.1
1.0
1.4


CC Gr.2 ascend colon (ODO3921)
0.2
0.3
0.9
0.5
0.7


CC Margin (ODO3921)
0.7
0.3
0.9
1.0
0.2


CC from Partial Hepatectomy (ODO4309) Mets
7.6
8.4
10.2
9.5
8.8


Liver Margin (ODO4309)
100.0
100.0
100.0
100.0
100.0


Colon mets to lung (OD04451-01)
0.4
0.3
0.3
1.5
0.4


Lung Margin (OD04451-02)
0.2
0.1
0.8
0.2
0.2


Normal Prostate 6546-1
0.4
0.0
0.2
0.1
0.1


Prostate Cancer (OD04410)
0.2
0.2
0.0
0.3
0.0


Prostate Margin (OD04410)
0.0
0.2
0.3
0.0
0.6


Prostate Cancer (OD04720-01)
0.6
0.1
0.2
0.2
0.3


Prostate Margin (OD04720-02)
0.5
0.5
0.6
0.3
0.2


Normal Lung 061010
5.6
5.2
6.3
8.1
5.9


Lung Met to Muscle (ODO4286)
0.0
0.0
0.0
0.0
0.0


Muscle Margin (ODO4286)
0.5
0.0
0.1
0.2
0.0


Lung Malignant Cancer (OD03126)
1.0
1.0
1.3
0.8
1.0


Lung Margin (OD03126)
0.9
1.1
0.9
1.4
1.4


Lung Cancer (OD04404)
1.3
0.8
1.6
1.5
1.4


Lung Margin (OD04404)
2.0
2.5
4.3
3.4
3.2


Lung Cancer (OD04565)
0.2
0.1
0.5
0.0
0.2


Lung Margin (OD04565)
0.3
0.0
0.3
0.4
0.7


Lung Cancer (OD04237-01)
0.4
0.9
1.2
1.5
1.2


Lung Margin (OD04237-02)
5.6
5.4
7.9
6.3
5.6


Ocular Mel Met to Liver (ODO4310)
0.2
0.0
0.0
0.1
0.4


Liver Margin (ODO4310)
52.9
64.6
79.6
81.8
63.3


Melanoma Mets to Lung (OD04321)
0.0
0.0
0.0
0.1
0.3


Lung Margin (OD04321)
0.2
2.0
0.9
1.5
0.5


Normal Kidney
0.5
0.3
0.3
0.7
0.3


Kidney Ca, Nuclear grade 2 (OD04338)
0.0
0.1
0.0
0.0
0.1


Kidney Margin (OD04338)
0.0
0.2
0.5
0.1
0.3


Kidney Ca Nuclear grade 1/2 (OD04339)
0.0
0.0
0.0
0.0
0.0


Kidney Margin (OD04339)
0.0
0.1
0.1
0.3
0.1


Kidney Ca, Clear cell type (OD04340)
0.0
0.1
0.0
0.0
0.1


Kidney Margin (OD04340)
0.2
0.1
0.8
0.0
0.0


Kidney Ca, Nuclear grade 3 (OD04348)
0.0
0.0
0.2
0.0
0.1


Kidney Margin (OD04348)
0.0
0.1
0.2
0.1
0.1


Kidney Cancer (OD04622-01)
0.2
0.1
0.1
1.2
0.2


Kidney Margin (OD04622-03)
0.0
0.1
0.0
0.0
0.0


Kidney Cancer (OD04450-01)
0.0
0.0
0.0
0.0
0.0


Kidney Margin (OD04450-03)
0.1
0.0
0.1
0.1
0.1


Kidney Cancer 8120607
0.0
0.0
0.0
0.0
0.0


Kidney Margin 8120608
0.1
0.0
0.2
0.0
0.0


Kidney Cancer 8120613
0.3
0.2
0.2
0.1
0.5


Kidney Margin 8120614
0.3
0.1
0.4
0.3
0.0


Kidney Cancer 9010320
0.0
0.1
0.1
0.1
0.2


Kidney Margin 9010321
0.1
0.0
0.0
0.1
0.2


Normal Uterus
0.5
0.1
0.8
0.1
0.5


Uterus Cancer 064011
0.8
0.7
1.3
0.4
0.6


Normal Thyroid
1.8
1.1
1.5
0.9
2.3


Thyroid Cancer 064010
0.0
0.0
0.0
0.0
0.0


Thyroid Cancer A302152
0.5
1.0
1.3
0.6
0.8


Thyroid Margin A302153
2.3
2.4
3.5
3.9
2.4


Normal Breast
9.0
6.8
9.0
9.9
5.5


Breast Cancer (OD04566)
0.1
0.0
0.1
0.0
0.0


Breast Cancer (OD04590-01)
0.3
0.4
0.2
0.7
0.2


Breast Cancer Mets (OD04590-03)
1.7
2.4
2.5
2.5
1.4


Breast Cancer Metastasis (OD04655-05)
10.7
13.2
22.1
15.6
12.8


Breast Cancer 064006
0.5
0.8
0.8
0.7
0.7


Breast Cancer 1024
4.1
2.5
5.1
3.1
3.6


Breast Cancer 9100266
0.9
0.2
0.2
0.2
0.2


Breast Margin 9100265
0.7
0.4
1.0
0.7
1.2


Breast Cancer A209073
1.2
1.2
1.1
1.9
0.8


Breast Margin A209073
1.5
1.0
2.1
1.5
0.6


Normal Liver
27.5
27.9
37.4
42.3
27.2


Liver Cancer 064003
0.8
0.5
0.3
0.6
0.5


Liver Cancer 1025
33.7
36.6
36.6
39.0
27.5


Liver Cancer 1026
4.6
3.6
5.7
5.8
5.2


Liver Cancer 6004-T
36.9
38.7
50.3
46.7
38.7


Liver Tissue 6004-N
1.5
1.0
1.3
1.3
1.6


Liver Cancer 6005-T
4.5
3.9
4.2
4.3
2.9


Liver Tissue 6005-N
22.2
24.5
32.8
27.5
28.7


Normal Bladder
5.3
3.4
4.8
4.2
5.5


Bladder Cancer 1023
1.6
1.3
1.5
1.6
1.7


Bladder Cancer A302173
0.9
0.4
1.5
1.0
0.3


Bladder Cancer (OD04718-01)
0.0
0.0
0.0
0.0
0.0


Bladder Normal Adjacent (OD04718-03)
5.2
6.2
7.8
6.0
4.1


Normal Ovary
1.6
3.0
5.4
3.6
3.6


Ovarian Cancer 064008
0.0
0.1
0.1
0.6
0.5


Ovarian Cancer (OD04768-07)
0.0
0.0
0.0
0.1
0.0


Ovary Margin (OD04768-08)
2.3
1.7
3.1
2.4
2.6


Normal Stomach
0.9
0.8
1.7
0.8
0.8


Gastric Cancer 9060358
0.0
0.4
0.8
0.4
0.2


Stomach Margin 9060359
1.2
0.8
1.3
1.0
0.9


Gastric Cancer 9060395
0.1
0.2
0.3
0.5
0.6


Stomach Margin 9060394
0.8
0.6
1.7
0.7
0.6


Gastric Cancer 9060397
0.1
0.1
0.1
0.0
0.0


Stomach Margin 9060396
0.3
0.1
0.6
0.1
0.3


Gastric Cancer 064005
0.7
0.6
0.4
1.1
0.3







Column A - Rel. Exp. (%) Ag2742, Run 153641758





Column B - Rel. Exp. (%) Ag2743, Run 153658357





Column C - Rel. Exp. (%) Ag2744, Run 153670751





Column D - Rel. Exp. (%) Ag2745, Run 153664739





Column E - Rel. Exp. (%) Ag2746, Run 153675220














TABLE HN










Panel 4D













Tissue Name
A
B
C
D
E
F
















Secondary Th1 act
0.0
0.0
0.0
0.0
0.0
0.0


Secondary Th2 act
0.0
0.7
0.0
0.0
0.0
0.0


Secondary Tr1 act
1.7
0.0
1.9
0.0
7.5
2.8


Secondary Th1 rest
0.0
0.0
0.0
0.0
0.0
0.0


Secondary Th2 rest
0.0
0.0
0.0
0.0
0.0
0.0


Secondary Tr1 rest
0.0
0.0
0.0
0.0
0.0
2.8


Primary Th1 act
0.0
0.0
0.0
0.0
0.0
0.0


Primary Th2 act
1.6
0.0
0.0
0.0
0.0
4.8


Primary Tr1 act
0.0
0.0
0.0
0.0
0.0
0.0


Primary Th1 rest
0.0
0.0
0.0
0.0
0.0
0.0


Primary Th2 rest
0.0
0.0
0.0
0.0
0.0
0.0


Primary Tr1 rest
0.0
0.0
0.0
0.0
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
0.0
0.0
0.0
0.0
0.0


CD45RO CD4 lymphocyte act
0.0
0.0
0.0
0.0
0.0
0.0


CD8 lymphocyte act
0.0
0.0
0.0
0.0
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0
0.0
0.0
0.0
0.0


Secondary CD8 lymphocyte act
0.7
0.0
0.0
7.8
0.0
1.8


CD4 lymphocyte none
0.0
0.0
0.0
0.0
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0
0.0
0.0
0.0
0.0


LAK cells rest
0.0
0.0
0.0
0.0
0.0
0.0


LAK cells IL-2
0.0
0.0
0.0
0.0
0.0
4.7


LAK cells IL-2 + IL-12
0.0
0.0
0.0
0.0
1.9
0.0


LAK cells IL-2 + IFN gamma
0.0
1.5
3.3
5.0
0.0
3.0


LAK cells IL-2 + IL-18
0.0
0.0
2.1
0.0
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0
0.0
0.0
0.0
0.0


NK Cells IL-2 rest
0.0
0.0
0.0
0.0
0.0
0.0


Two Way MLR 3 day
0.0
0.0
0.0
0.0
0.0
0.0


Two Way MLR 5 day
0.0
0.0
0.0
0.0
0.0
0.0


Two Way MLR 7 day
2.0
0.0
6.4
0.0
0.0
11.3


PBMC rest
0.0
0.0
0.0
0.0
0.0
5.1


PBMC PWM
0.0
2.3
0.0
0.0
0.0
2.7


PBMC PHA-L
0.0
1.5
2.5
0.0
0.0
2.2


Ramos (B cell) none
0.0
0.0
0.0
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0
0.0
0.0
0.0


B lymphocytes PWM
0.0
0.0
0.0
0.0
0.0
2.0


B lymphocytes CD40L and IL-4
0.0
0.0
0.0
0.0
0.0
6.0


EOL-1 dbcAMP
0.0
0.0
0.0
0.0
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0
0.0
0.0
0.0


Dendritic cells none
1.4
2.9
0.0
0.0
4.9
0.0


Dendritic cells LPS
0.0
0.0
0.0
0.0
0.0
0.0


Dendritic cells anti-CD40
0.0
0.0
3.3
0.0
0.0
0.0


Monocytes rest
0.0
0.0
0.0
0.0
0.0
0.0


Monocytes LPS
0.0
0.0
0.0
0.0
0.0
0.0


Macrophages rest
0.0
0.0
0.0
0.0
0.0
0.0


Macrophages LPS
0.0
0.0
0.0
2.4
0.0
0.0


HUVEC none
0.0
0.0
0.0
0.0
0.0
0.0


HUVEC starved
0.0
0.0
0.0
0.0
0.0
0.0


HUVEC IL-1beta
0.0
0.0
0.0
0.0
0.0
0.0


HUVEC IFN gamma
3.7
0.0
0.0
0.0
2.7
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0
0.0
0.0
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0
0.0
0.0
0.0
0.0


HUVEC IL-11
1.4
0.0
0.0
0.0
0.0
2.6


Lung Microvascular EC none
1.6
0.0
2.2
0.0
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
3.1
2.9
0.0
0.0
0.0
0.0


Microvascular Dermal EC none
0.0
3.1
5.0
0.0
0.0
12.6


Microsvasular Dermal EC TNFalpha + IL-1beta
1.4
1.1
0.0
0.0
1.6
0.0


Bronchial epithelium TNFalpha + IL1beta
0.0
0.0
0.0
0.0
0.0
0.0


Small airway epithelium none
0.0
0.0
0.0
0.0
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
0.0
0.0
0.0
0.0
0.0
0.0


Coronery artery SMC rest
0.0
0.0
0.0
0.0
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0
0.0
0.0
0.0
0.0


Astrocytes rest
0.0
0.0
0.0
0.0
0.0
0.0


Astrocytes TNFalpha + IL-1 beta
0.0
0.0
0.0
0.0
0.0
0.0


KU-812 (Basophil) rest
0.0
0.0
0.0
0.0
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0
0.0
0.0
0.0
0.0


CCD1106 (Keratinocytes) none
0.0
0.0
0.0
0.0
0.0
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0
0.0
0.0
0.0
0.0
0.0


Liver cirrhosis
100.0
100.0
100.0
100.0
100.0
100.0


Lupus kidney
0.0
1.4
0.0
0.0
0.0
0.0


NCI-H292 none
0.0
0.0
0.0
0.0
0.0
0.0


NCI-H292 IL-4
0.0
0.0
0.0
0.0
2.3
0.0


NCI-H292 IL-9
0.0
0.0
0.0
0.0
0.0
0.0


NCI-H292 IL-13
0.0
0.0
0.0
0.0
0.0
0.0


NCI-H292 IFN gamma
0.0
0.0
0.0
0.0
0.0
0.0


HPAEC none
4.8
1.4
0.0
0.0
0.0
3.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0
0.0
0.0
0.0
0.0


Lung fibroblast none
0.0
0.0
0.0
0.0
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0
0.0
0.0
0.0
0.0


Lung fibroblast IL-4
0.0
0.0
0.0
0.0
0.0
0.0


Lung fibroblast IL-9
0.0
0.0
0.0
0.0
0.0
0.0


Lung fibroblast IL-13
0.0
0.0
0.0
0.0
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0
0.0
0.0
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0
0.0
0.0
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
0.0
0.0
0.0
0.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.0
0.0
0.0
0.0
0.0


Dermal fibroblast IFN gamma
0.0
0.0
0.0
0.0
0.0
5.3


Dermal fibroblast IL-4
0.0
0.0
0.0
0.0
0.0
0.0


IBD Colitis 2
1.7
0.0
0.0
0.0
0.0
0.0


IBD Crohn's
4.5
1.6
6.7
6.1
3.3
3.0


Colon
5.5
2.1
23.0
8.3
4.7
11.1


Lung
41.5
26.2
27.7
39.2
37.1
49.7


Thymus
4.2
0.0
4.1
0.0
1.4
20.6


Kidney
4.7
5.2
8.7
5.9
6.3
6.7







Column A - Rel. Exp. (%) Ag2742, Run 153641803





Column B - Rel. Exp. (%) Ag2743, Run 153658360





Column C - Rel. Exp. (%) Ag2744, Run 153670759





Column D - Rel. Exp. (%) Ag2745, Run 153664740





Column E - Rel. Exp. (%) Ag2746, Run 153675321





Column F - Rel. Exp. (%) Ag812, Run 138175358














TABLE HO










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
2.9



Colon NAT 1
3.9



Colon cancer 2
10.2



Colon NAT 2
5.0



Colon cancer 3
4.8



Colon NAT 3
10.2



Colon malignant cancer 4
0.0



Colon NAT 4
3.8



Lung cancer 1
5.0



Lung NAT 1
1.3



Lung cancer 2
9.0



Lung NAT 2
0.0



Squamous cell carcinoma 3
6.1



Lung NAT 3
0.0



Metastatic melanoma 1
43.8



Melanoma 2
0.5



Melanoma 3
4.0



Metastatic melanoma 4
82.4



Metastatic melanoma 5
100.0



Bladder cancer 1
1.8



Bladder NAT 1
0.0



Bladder cancer 2
0.7



Bladder NAT 2
0.7



Bladder NAT 3
0.0



Bladder NAT 4
9.0



Prostate adenocarcinoma 1
0.7



Prostate adenocarcinoma 2
0.6



Prostate adenocarcinoma 3
0.9



Prostate adenocarcinoma 4
0.9



Prostate NAT 5
2.3



Prostate adenocarcinoma 6
0.0



Prostate adenocarcinoma 7
0.0



Prostate adenocarcinoma 8
1.0



Prostate adenocarcinoma 9
0.7



Prostate NAT 10
0.2



Kidney cancer 1
1.1



Kidney NAT 1
1.6



Kidney cancer 2
16.2



Kidney NAT 2
3.0



Kidney cancer 3
4.7



Kidney NAT 3
0.0



Kidney cancer 4
0.0



Kidney NAT 4
0.0









Column A - Rel. Exp. (%) Ag812, Run 262228032







Panel 1 Summary: Ag03/Ag068 Highest expression of this gene was detected in the spleen (CTs=21-25). This gene was more highly expressed in normal tissue than in cancer cell lines. There were however detectable levels of expression in cell lines derived from melanoma, breast, renal, ovarian, lung, gastric and colon cancers. The difference in levels of expression of this gene is useful for differentiating between these cancer cell line samples and other samples on this panel and between normal tissues and malignancies from those cancers.


There were also higher levels of expression in lung, and kidney tissue from fetal sources (CTs=25-28) when compared to levels of expression in the adult (CTs=38-31). 10 Expression of this gene or its protein product is useful for differentiating between adult and fetal lung and kidney tissue.


Among tissues with metabolic function, this gene was expressed in the liver, pituitary, thyroid, heart, skeletal muscle and adrenal gland. This demonstrates that the protein encoded by this gene is involved in the homeostasis of these tissues. Therapeutic 15 modulation of the expression or function of this gene product is effective in the treatment of metabolic disorders, including obesity and diabetes.


This gene is a homolog of Stablin-1, and was also expressed at moderate levels in all brain regions examined. Because stablin is involved in angiogenesis, the therapeutic modulation of this gene or its protein product is of benefit in the treatment of 20 stroke/cerebral ischemia/cerebral infarct.


Panel 1.2 Summary: Ag812 Highest expression of this gene was detected in the liver (CTs=25). Significant expression was also found in other metabolic tissues including fetal and adult heart, skeletal muscle, pancreas, thyroid, pituitary and adrenal gland. The high expression of this gene in the liver shows that this gene is involved in the normal homeostasis of that organ. Therapeutic modulation of the expression or function of this gene or protein product is effective in the treatment of diseases that involve the liver.


This gene also showed low to moderate expression in the brain. Please see Panel 1 for discussion of potential utility of this gene in the central nervous system.


While this gene showed a greater association for normal tissue, there were significant levels of expression in a cluster of ovarian cancer cell lines. Expression of this gene is useful for differentiating between those samples and other samples on this panel, and between normal and malignant ovarian tissue. Therapeutic modulation of the expression or function of this gene or protein product is effective in the treatment of ovarian cancer.


Panel 1.3D Summary: Ag2742, Ag2743, Ag2744, Ag2745, Ag2746 Highest expression of this gene was detected in the liver (CTs=25). Significant expression was also found in the spleen (CTs=28-29). This result was in concordance with the results from Panel 1.


This gene was expressed at higher levels in the fetal kidney and skeletal muscle (CTs=32-34) than in the comparable adult tissues (CTs=40). Thus, expression of this gene is useful for differentiating between kidney and skeletal muscle tissue from adult and fetal sources. The higher levels of expression of this gene in the fetal tissues show that this gene product is involved in the development of these organs. Therapeutic modulation of the expression or function of these genes or their protein products is effective in treating disease of these organs in the adult.


In this panel, expression this gene was exclusively associate with normal tissue samples, a preference that was also observed in panels 1 and 1.2. Absence of expression of this gene is useful in differentiating between the cancerous cell lines on this panel, and their corresponding normal tissues.


Panel 2D Summary: Ag2742/Ag2743/Ag2744/Ag2745/Ag2746 Expression of this gene was highest and almost exclusive in the liver (CTs=27-29). This result was in concordance with the results from previous panels. The low/undetectable levels of expression in cancer samples was also in agreement with the results observed in the preceding experiments. Expression of this gene or its protein product is useful for differentiating between liver tissue and other samples on this panel and as a marker for liver tissue. Therapeutic modulation of the expression or function of the gene or the protein encoded by this gene is effective in the treatment of liver cancer or other disease that involve the liver. This gene is useful as a marker for breast cancer metastasis as it shows elevated expression in a metastatic breast cancer compared to expression in the primary cancer.


Panel 4D Summary: Ag812/Ag2742/Ag2743/Ag2744/Ag2745/Ag2746 The expression of this gene appears was highest in samples from cirrhotic liver, (CTs=32-33). Low level expression was also detected in samples derived from normal lung. The presence of this gene in liver cirrhosis (a component of which involves liver inflammation and fibrosis) shows that therapeutic agents involving this gene or its protein product are useful in reducing or inhibiting the inflammation associated with fibrotic and other inflammatory diseases.


general oncology screening panel_V—2.4 Summary: Ag812 Highest expression of this gene was seen in metastatic melanoma (CTs=31). Gene, protein, antibodies or small molecule therapeutics that target this gene or its protein product are useful in the treatment of melanoma.


I. CG50925-01 and CG50925-02: TENM5


Expression of gene CG50925-01 and variant CG50925-02 was assessed using the primer-probe set Ag1402 described in Table IA. Results of the RTQ-PCR runs are shown in Tables B, IC, ID, IE, IF, IG, and IH.

TABLE IAProbe Name Ag1402StartSEQ IDPrimersSequencesLengthPositionNoForward5′-cttacaacaccacgggactaag-3′2255461291ProbeTET-5′-aagagcacttccttgcctccgtaagg-3′-2655691292TAMRAReverse5′-agatcaaatgattgggatggtt-3′2256051293









TABLE IB










HASS Panel v1.0










Tissue Name
A














MCF-7 C1
2.4



MCF-7 C2
4.2



MCF-7 C3
3.6



MCF-7 C4
4.9



MCF-7 C5
4.2



MCF-7 C6
5.2



MCF-7 C7
3.3



MCF-7 C9
3.4



MCF-7 C10
6.7



MCF-7 C11
0.9



MCF-7 C12
5.2



MCF-7 C13
1.6



MCF-7 C15
2.6



MCF-7 C16
6.3



MCF-7 C17
7.5



T24 D1
0.5



T24 D2
2.0



T24 D3
2.0



T24 D4
1.8



T24 D5
2.5



T24 D6
2.4



T24 D7
1.1



T24 D9
6.6



T24 D10
2.2



T24 D11
2.2



T24 D12
3.1



T24 D13
2.0



T24 D15
6.2



T24 D16
1.1



T24 D17
1.7



CAPaN B1
2.3



CAPaN B2
0.7



CAPaN B3
0.3



CAPaN B4
0.3



CAPaN B5
1.2



CAPaN B6
1.1



CAPaN B7
0.4



CAPaN B8
0.5



CAPaN B9
0.8



CAPaN B10
0.9



CAPaN B11
0.8



CAPaN B12
0.6



CAPaN B13
0.7



CAPaN B14
1.4



CAPaN B15
0.4



CAPaN B16
0.6



CAPaN B17
1.2



U87-MG F1 (B)
1.0



U87-MG F2
2.7



U87-MG F3
5.6



U87-MG F4
2.9



U87-MG F5
6.7



U87-MG F6
7.3



U87-MG F7
4.5



U87-MG F8
11.7



U87-MG F9
3.4



U87-MG F10
5.0



U87-MG F11
12.9



U87-MG F12
7.9



U87-MG F13
9.7



U87-MG F14
24.3



U87-MG F15
10.7



U87-MG F16
6.8



U87-MG F17
11.4



LnCAP A1
0.9



LnCAP A2
2.0



LnCAP A3
2.2



LnCAP A4
2.5



LnCAP A5
2.0



LnCAP A6
1.8



LnCAP A7
5.3



LnCAP A8
5.3



LnCAP A9
3.0



LnCAP A10
1.8



LnCAP A11
6.2



LnCAP A12
0.5



LnCAP A13
0.5



LnCAP A14
0.8



LnCAP A15
0.8



LnCAP A16
1.7



LnCAP A17
4.6



Primary Astrocytes
27.0



Primary Renal Proximal Tubule Epithelial cell A2
0.8



Primary melanocytes A5
100.0



126443 - 341 medullo
0.6



126444 - 487 medullo
28.1



126445 - 425 medullo
1.2



126446 - 690 medullo
8.8



126447 - 54 adult glioma
28.1



126448 - 245 adult glioma
76.3



126449 - 317 adult glioma
9.5



126450 - 212 glioma
5.9



126451 - 456 glioma
27.0









Column A - Rel. Exp. (%) Ag1402, Run 318641615














TABLE IC










Human Metabolic










Tissue Name
A














137857_psoas-AA.M.Diab.-hi BMI-6
0.5



135760_psoas-HI.M.Diab.-hi BMI-21
1.4



134827_psoas-CC.M.Diab.-hi BMI-4
2.1



137860_psoas-AA.M.Diab.-med BMI-8
1.1



137834_psoas-CC.M.Diab.-med BMI-2
2.8



137828_psoas-CC.M.Diab.-med BMI-1
2.1



135763_psoas-HI.M.Diab.-med BMI-23
1.2



142740_psoas-AS.M.Diab.-low BMI-20
18.4



134834_psoas-AA.M.Diab.-low BMI-17
1.2



137850_psoas-AS.M.Norm-hi BMI-34
1.8



135769_psoas-HI.M.Norm-hi BMI-31
0.6



135766_psoas-AA.M.Norm-hi BMI-25
1.3



142746_psoas-AA.M.Norm-med BMI-37
38.2



142745_psoas-HI.M.Norm-med BMI-35
15.7



137855_psoas-AA.M.Norm-med BMI-47
0.9



137844_psoas-CC.M.Norm-med BMI-26
0.5



142742_psoas-CC.M.Norm-low BMI-40
29.9



137873_psoas-AS.M.Norm-low BMI-28
0.6



137853_psoas-HI.M.Norm-low BMI-41
0.9



135775_psoas-CC.M.Norm-low BMI-39
0.5



137858_diaphragm-AA.M.Diab.-hi BMI-6
1.6



135772_diaphragm-AS.M.Diab-hi BMI-9
2.1



135761_diaphragm-HI.M.Diab.-hi BMI-21
5.9



134828_diaphragm-CC.M.Diab.-hi BMI-4
1.1



137835_diaphragm-CC.M.Diab.-med BMI-2
1.6



135764_diaphragm-HI.M.Diab.-med BMI-23
1.8



134835_diaphragm-AA.M.Diab.-low BMI-17
1.8



142738_diaphragm-CC.M.Norm-hi BMI-29
2.1



139517_diaphragm-AS.M.Norm-hi BMI-34
3.3



137848_diaphragm-HI.M.Norm-hi BMI-31
0.9



137843_diaphragm-AA.M.Norm-hi BMI-25
1.7



137879_diaphragm-AA.M.Norm-med BMI-47
1.7



137872_diaphragm-CC.M.Norm-med BMI-26
1.2



135773_diaphragm-HI.M.Norm-med BMI-35
51.1



139542_diaphragm-HI.M.Norm-low BMI-41
1.9



137877_diaphragm-CC.M.Norm-low BMI-39
1.2



137874_diaphragm-AS.M.Norm-low BMI-28
0.9



141340_subQadipose-AA.M.Diab.-hi BMI-6
15.8



137836_subQadipose-HI.M.Diab.-hi BMI-21
2.1



135771_subQadipose-AS.M.Diab-hi BMI-9
4.0



141329_subQadipose-AA.M.Diab-medbmi-8
3.1



137862_subQadipose-CC.M.Diab.-med BMI-1
3.8



135762_subQadipose-HI.M.Diab.-med BMI-23
1.1



141338_subQadipose-AS.M.Diab.-low BMI-20
1.4



139547_subQadipose-HI.M.Diab.-low BMI-22
4.4



135757_subQadipose-CC.M.Diab.-low BMI-13
2.4



134832_subQadipose-AA.M.Diab.-low BMI-17
2.8



141332_subQadipose-HI.M.Norm-hi BMI-31
4.0



135767_subQadipose-CC.M.Norm-hi BMI-29
0.4



135765_subQadipose-AS.M.Norm-hi BMI-34
0.9



141339_subQadipose-HI.M.Norm-med BMI-35
6.0



141334_subQadipose-CC.M.Norm-med BMI-26
1.5



139544_subQadipose-AA.M.Norm-med BMI-47
3.3



137875_subQadipose-AA.M.Norm-med BMI-37
1.9



141331_subQadipose-AS.M.Norm-low BMI-28
2.1



137878_subQadipose-HI.M.Norm-low BMI-41
2.2



137876_subQadipose-CC.M.Norm-low BMI-39
2.1



137859_vis.adipose-AA.M.Diab.-hi BMI-6
0.7



135770_vis.adipose-AS.M.Diab-hi BMI-9
5.6



135759_vis.adipose-HI.M.Diab.-hi BMI-21
0.9



143502_vis.adipose-CC.M.Diab.-med BMI-2
9.8



139510_vis.adipose-AA.M.Diab.-med BMI-8
1.4



137861_vis.adipose-CC.M.Diab.-med-1
2.2



137839_vis.adipose-HI.M.Diab.-med BMI-23
1.5



139546_vis.adipose-HI.M.Diab.-low BMI-22
7.3



137831_vis.adipose-CC.M.Diab.-low BMI-13
1.6



139522_vis.adipose-HI.M.Norm-hi BMI-31
2.7



139516_vis.adipose-AS.M.Norm-hi BMI-34
33.9



137846_vis.adipose-CC.M.Norm-hi BMI-29
0.2



137841_vis.adipose-AA.M.Norm-hi BMI-25
0.7



139543_vis.adipose-AA.M.Norm-med BMI-47
20.2



139532_vis.adipose-AA.M.Norm-med BMI-37
2.2



139530_vis.adipose-HI.M.Norm-med BMI-35
1.5



139539_vis.adipose-HI.M.Norm-low BMI-41
2.0



139535_vis.adipose-CC.M.Norm-low BMI-40
13.1



137852_vis.adipose-CC.M.Norm-low BMI-39
2.8



135768_vis.adipose-AS.M.Norm-low BMI-28
0.5



141327_liver-CC.M.Diab.-hi BMI-4
1.1



139514_liver-HI.M.Diab.-hi BMI-21
2.0



139526_liver-CC.M.Diab.-med BMI-2
2.2



139511_liver-AA.M.Diab.-med BMI-8
2.0



137840_liver-HI.M.Diab.-med BMI-23
1.1



137827_liver-CC.M.Diab.-med BMI-1
0.8



137838_liver-HI.M.Diab.-low BMI-22
0.4



135758_liver-CC.M.Diab.-low BMI-13
0.6



139519_liver-CC.M.Norm-hi BMI-29
1.9



139518_liver-AA.M.Norm-hi BMI-25
1.9



137849_liver-AS.M.Norm-hi BMI-34
1.1



137847_liver-HI.M.Norm-hi BMI-31
0.5



142741_liver-AA.M.Norm-med BMI-37
1.8



141341_liver-HI.M.Norm-med BMI-35
1.1



141335_liver-CC.M.Norm-med BMI-26
1.3



139540_liver-HI.M.Norm-low BMI-41
7.1



139534_liver-CC.M.Norm-low BMI-39
5.8



139521_liver-AS.M.Norm-low BMI-28
0.8



141328_pancreas-CC.M.Diab.-hi BMI-4
28.9



139525_pancreas-AS.M.Diab.-hi BMI-9
5.1



137856_pancreas-AA.M.Diab.-hi BMI-6
0.3



137837_pancreas-HI.M.Diab.-hi BMI-21
1.3



141337_pancreas-CC.M.Diab.-med BMI-2
4.4



139527_pancreas-CC.M.Diab.-med BMI-1
7.9



139515_pancreas-HI.M.Diab.-med BMI-23
27.0



139512_pancreas-AA.M.Diab.-med BMI-8
40.3



142739_pancreas-AS.M.Diab.-low BMI-20
25.9



139513_pancreas-CC.M.Diab.-low BMI-13
16.8



142743_pancreas-AA.M.Norm-hi BMI-25
11.0



139523_pancreas-HI.M.Norm-hi BMI-31
16.7



139520_pancreas-CC.M.Norm-hi BMI-29
27.9



142744_pancreas-HI.M.Norm-med BMI-35
3.4



139545_pancreas-AA.M.Norm-med BMI-47
27.9



139531_pancreas-AA.M.Norm-med BMI-37
8.1



137871_pancreas-CC.M.Norm-med BMI-26
1.3



139541_pancreas-Hi.M.Norm-low BMI-41
16.5



139537_pancreas-CC.M.Norm-low BMI-40
14.7



139533_pancreas-CC.M.Norm-low BMI-39
2.8



137845_pancreas-AS.M.Norm-low BMI-28
0.9



143530_small intestine-AA.M.Diab.-hi BMI-6
7.7



143529_small intestine-CC.M.Diab.-hi BMI-4
35.1



143538_small intestine-HI.M.Diab.-med BMI-23
55.1



143531_small intestine-AA.M.Diab.-med BMI-8
44.1



143528_small intestine-CC.M.Diab.-med BMI-2
28.7



143537_small intestine-HI.M.Diab.-low BMI-22
17.6



143535_small intestine-AS.M.Diab.-low BMI-20
16.7



143534_small intestine-AA.M.Diab.-low BMI-17
0.8



143544_small intestine-AS.M.Norm-hi BMI-34
16.8



143543_small intestine-HI.M.Norm-hi BMI-31
3.7



143542_small intestine-CC.M.Norm-hi BMI-29
10.3



143539_small intestine-AA.M.Norm-hi BMI-25
31.9



143548_small intestine-AA.M.Norm-med BMI-47
0.0



143547_small intestine-AA.M.Norm-med BMI-37
24.5



143540_small intestine-CC.M.Norm-med BMI-26
3.9



143550_small intestine-CC.M.Norm-low BMI-40
1.4



143549_small intestine-CC.M.Norm-low BMI-39
24.0



143546_small intestine-HI.M.Norm-low BMI-41
4.7



143525_hypothalamus-HI.M.Diab.-hi BMI-21
22.8



143515_hypothalamus-CC.M.Diab.-hi BMI-4
8.4



143513_hypothalamus-AA.M.Diab.-hi BMI-6
1.5



143507_hypothalamus-AS.M.Diab.-hi BMI-9
1.8



143506_hypothalamus-CC.M.Diab.-med BMI-1
3.9



143505_hypothalamus-HI.M.Diab.-med BMI-23
31.0



143509_hypothalamus-AA.M.Diab.-low BMI-17
2.6



143508_hypothalamus-CC.M.Diab.-low BMI-13
2.4



143503_hypothalamus-AS.M.Diab.-low BMI-20
2.7



143522_hypothalamus-HI.M.Norm-hi BMI-31
1.5



143516_hypothalamus-AS.M.Norm-hi BMI-34
1.1



143511_hypothalamus-CC.M.Norm-hi BMI-29
2.4



143504_hypothalamus-AA.M.Norm-hi BMI-25
1.6



143517_hypothalamus-AA.M.Norm-med BMI-47
12.9



143514_hypothalamus-HI.M.Norm-med BMI-35
1.0



143521_hypothalamus-AS.M.Norm-low BMI-28
11.0



143512_hypothalamus-CC.M.Norm-low BMI-40
10.3



145454_Patient-25pl (CC.Diab.low BMI.no insulin)
29.1



110916_Patient-18pl (HI.Diab.obese.no insulin)
11.5



110913_Patient-18go (HI.Diab.obese.no insulin)
15.7



110911_Patient-17pl (CC.Diab.low BMI.no insulin)
6.7



110908_Patient-17go (CC.Diab.low BMI.no insulin)
14.0



100752_Patient-15sk (CC.Diab.obese.no insulin)
5.9



97828_Patient-13pl (CC.Diab.overwt.no insulin)
16.6



160114_Patient 27-ut (CC.Diab.obese.insulin)
34.4



160113_Patient 27-pl (CC.Diab.obese.insulin)
20.2



160112_Patient 27-sk (CC.Diab.obese.insulin)
2.9



160111_Patient 27-go (CC.Diab.obese.insulin)
11.7



145461_Patient-26sk (CC.Diab.obese.insulin)
4.8



145441_Patient-22sk (CC.Diab.low BMI.insulin)
3.3



145438_Patient-22pl (CC.Diab.low BMI.insulin)
30.6



145427_Patient-20pl (CC.Diab.overwt.insulin)
46.7



97503_Patient-12pl (CC.Diab.unknown
2.2



BMI.insulin)



145443_Patient-23pl (CC.Non-diab.overwt)
34.9



145435_Patient-21pl (CC.Non-diab.overwt)
42.0



110921_Patient-19pl (CC.Non-diab.low BMI)
16.3



110918_Patient-19go (CC.Non-diab.low BMI)
8.5



97481_Patient-08sk (CC.Non-diab.obese)
10.4



97478_Patient-07pl (CC.Non-diab.obese)
9.2



160117_Human Islets-male, obese
0.9



145474_PANC1 (pancreas carcinoma) 1
1.5



154911_Capan2 (pancreas adenocarcinoma)
1.2



141190_SW579 (thyroid carcinoma)
25.3



145489_SK-N-MC (neuroblastoma) 1
75.8



145495_SK-N-SH (neuroblastoma) 1
34.4



145498_U87 MG (glioblastoma) 2
1.4



145484_HEp-2 (larynx carcinoma) 1
2.6



145479_A549 (lung carcinoma)
3.3



145488_A427 (lung carcinoma) 2
2.4



145472_FHs 738Lu (normal lung) 1
59.0



141187_SKW6.4 (B lymphocytes)
0.5



154644_IM-9 (immunoglobulin secreting
0.3



lymphoblast)



154645_MOLT-4 (acute lymphoblastic leukemia
1.3



derived from peripheral blood)



154648_U-937 (histiocystic lymphoma)
100.0



154647_Daudi (Burkitt's lymphoma)
0.6



145494_SK-MEL-2 (melanoma) 2
11.7



141176_A375 (melanoma)
2.9



154642_SW 1353 (humerus chondrosarcoma)
13.8



141179_HT-1080 (fibrosarcoma)
4.3



145491_MG-63 (osteosarcoma) 1
35.4



141186_MCF7 (breast carcinoma)
5.3



141193_T47D (breast carcinoma)
1.9



154641_BT-20 (breast carcinoma)
0.8



141175_293 (kidney transformed with adenovirus
1.3



5 DNA)



141182_HUH hepatoma 1
2.4



141184_HUH7 hepatoma 1
3.2



145478_HT1376 (bladder carcinoma)
0.3



145481_SCaBER (bladder carcinoma)
1.0



141192_SW620 (lymph node metastatsis, colon
1.8



carcinoma) 2



141180_HT29 (colon carcinoma) 1
0.5



141188_SW480 (colon carcinoma) 1
0.9



154646_CAOV-3 (ovary adenocarcinoma)
1.0



141194_HeLa (cervix carcinoma)- 2
2.2



145482_HeLa S3 (cervix carcinoma) 1
1.4



145486_DU145 (prostate carcinoma)
1.1



154643_PC-3 (prostate adenocarcinoma)
2.0



154649_HCT-8 (ileocecal adenocarcinoma)
5.9









Column A - Rel. Exp. (%) Ag1402, Run 390904156














TABLE ID










Panel 1.2











Tissue Name
A
B















Endothelial cells
15.3
15.8



Heart (Fetal)
45.4
33.9



Pancreas
1.3
0.5



Pancreatic ca. CAPAN 2
0.2
0.5



Adrenal Gland
22.2
17.7



Thyroid
1.5
0.5



Salivary gland
12.0
10.4



Pituitary gland
1.7
2.5



Brain (fetal)
0.2
0.3



Brain (whole)
1.3
1.6



Brain (amygdala)
3.0
3.0



Brain (cerebellum)
2.8
0.8



Brain (hippocampus)
9.5
9.5



Brain (thalamus)
5.8
5.6



Cerebral Cortex
15.4
12.3



Spinal cord
1.5
1.1



glio/astro U87-MG
1.2
1.4



glio/astro U-118-MG
6.7
7.7



astrocytoma SW1783
1.8
2.3



neuro*; met SK-N-AS
32.1
18.9



astrocytoma SF-539
43.5
30.4



astrocytoma SNB-75
2.9
2.7



glioma SNB-19
1.7
1.1



glioma U251
7.0
5.8



glioma SF-295
24.8
19.5



Heart
100.0
100.0



Skeletal Muscle
19.6
16.4



Bone marrow
1.7
2.2



Thymus
1.6
0.9



Spleen
10.2
7.6



Lymph node
1.4
1.3



Colorectal Tissue
1.6
4.2



Stomach
5.1
4.7



Small intestine
40.1
35.6



Colon ca. SW480
1.2
0.5



Colon ca.* SW620 (SW480 met)
3.1
2.0



Colon ca. HT29
0.2
0.2



Colon ca. HCT-116
5.6
3.0



Colon ca. CaCo-2
1.7
0.9



Colon ca. Tissue (ODO3866)
2.2
2.3



Colon ca. HCC-2998
8.7
4.6



Gastric ca.* (liver met) NCI-N87
1.2
1.3



Bladder
20.9
18.6



Trachea
1.4
0.8



Kidney
27.5
30.6



Kidney (fetal)
46.3
13.7



Renal ca. 786-0
0.5
0.4



Renal ca. A498
1.6
1.4



Renal ca. RXF 393
2.5
2.3



Renal ca. ACHN
1.7
2.1



Renal ca. UO-31
1.6
1.1



Renal ca. TK-10
3.3
3.3



Liver
13.2
12.2



Liver (fetal)
8.3
6.2



Liver ca. (hepatoblast) HepG2
2.5
0.8



Lung
1.5
1.4



Lung (fetal)
12.2
5.4



Lung ca. (small cell) LX-1
3.3
1.9



Lung ca. (small cell) NCI-H69
3.2
2.7



Lung ca. (s. cell var.) SHP-77
0.9
0.5



Lung ca. (large cell)NCI-H460
2.8
2.1



Lung ca. (non-sm. cell) A549
3.1
1.5



Lung ca. (non-s. cell) NCI-H23
2.3
3.4



Lung ca. (non-s. cell) HOP-62
5.3
11.7



Lung ca. (non-s. cl) NCI-H522
11.3
9.3



Lung ca. (squam.) SW 900
1.2
1.1



Lung ca. (squam.) NCI-H596
2.0
2.0



Mammary gland
4.2
29.5



Breast ca.* (pl. ef) MCF-7
0.6
0.7



Breast ca.* (pl. ef) MDA-MB-231
0.3
0.2



Breast ca.* (pl. ef) T47D
3.5
2.5



Breast ca. BT-549
0.4
0.4



Breast ca. MDA-N
12.2
14.2



Ovary
79.6
54.3



Ovarian ca. OVCAR-3
1.0
0.8



Ovarian ca. OVCAR-4
1.8
0.0



Ovarian ca. OVCAR-5
7.6
4.5



Ovarian ca. OVCAR-8
20.4
12.2



Ovarian ca. IGROV-1
1.4
1.1



Ovarian ca. (ascites) SK-OV-3
14.4
11.2



Uterus
34.4
19.6



Placenta
6.7
4.8



Prostate
70.2
74.2



Prostate ca.* (bone met) PC-3
7.9
8.5



Testis
1.2
0.6



Melanoma Hs688(A).T
15.8
15.3



Melanoma* (met) Hs688(B).T
33.0
26.6



Melanoma UACC-62
9.9
8.8



Melanoma M14
3.3
2.9



Melanoma LOX IMVI
0.8
1.0



Melanoma* (met) SK-MEL-5
5.8
6.1









Column A - Rel. Exp. (%) Ag1402, Run 138249713






Column B - Rel. Exp. (%) Ag1402, Run 138253129














TABLE IE










Panel 2.2










Tissue Name
A














Normal Colon
29.9



Colon cancer (OD06064)
41.2



Colon Margin (OD06064)
34.2



Colon cancer (OD06159)
2.6



Colon Margin (OD06159)
30.1



Colon cancer (OD06297-04)
7.0



Colon Margin (OD06297-05)
36.6



CC Gr.2 ascend colon (ODO3921)
7.2



CC Margin (ODO3921)
8.7



Colon cancer metastasis (OD06104)
2.7



Lung Margin (OD06104)
14.3



Colon mets to lung (OD04451-01)
1.8



Lung Margin (OD04451-02)
17.6



Normal Prostate
86.5



Prostate Cancer (OD04410)
12.4



Prostate Margin (OD04410)
32.3



Normal Ovary
74.7



Ovarian cancer (OD06283-03)
8.5



Ovarian Margin (OD06283-07)
34.4



Ovarian Cancer 064008
17.3



Ovarian cancer (OD06145)
17.3



Ovarian Margin (OD06145)
25.0



Ovarian cancer (OD06455-03)
6.4



Ovarian Margin (OD06455-07)
100.0



Normal Lung
18.9



Invasive poor diff. lung adeno (ODO4945-01
7.7



Lung Margin (ODO4945-03)
20.3



Lung Malignant Cancer (OD03126)
8.8



Lung Margin (OD03126)
7.7



Lung Cancer (OD05014A)
8.8



Lung Margin (OD05014B)
29.1



Lung cancer (OD06081)
11.1



Lung Margin (OD06081)
6.5



Lung Cancer (OD04237-01)
8.7



Lung Margin (OD04237-02)
27.7



Ocular Melanoma Metastasis
3.1



Ocular Melanoma Margin (Liver)
9.3



Melanoma Metastasis
10.3



Melanoma Margin (Lung)
18.2



Normal Kidney
10.6



Kidney Ca, Nuclear grade 2 (OD04338)
24.5



Kidney Margin (OD04338)
4.9



Kidney Ca Nuclear grade 1/2 (OD04339)
5.2



Kidney Margin (OD04339)
10.7



Kidney Ca, Clear cell type (OD04340)
12.9



Kidney Margin (OD04340)
10.1



Kidney Ca, Nuclear grade 3 (OD04348)
14.6



Kidney Margin (OD04348)
32.3



Kidney malignant cancer (OD06204B)
2.2



Kidney normal adjacent tissue (OD06204E)
9.1



Kidney Cancer (OD04450-01)
2.9



Kidney Margin (OD04450-03)
7.8



Kidney Cancer 8120613
1.2



Kidney Margin 8120614
9.5



Kidney Cancer 9010320
5.8



Kidney Margin 9010321
3.7



Kidney Cancer 8120607
16.6



Kidney Margin 8120608
6.0



Normal Uterus
88.9



Uterine Cancer 064011
14.3



Normal Thyroid
7.6



Thyroid Cancer 064010
2.2



Thyroid Cancer A302152
11.6



Thyroid Margin A302153
3.9



Normal Breast
95.3



Breast Cancer (OD04566)
4.0



Breast Cancer 1024
58.6



Breast Cancer (OD04590-01)
19.3



Breast Cancer Mets (OD04590-03)
22.7



Breast Cancer Metastasis (OD04655-05)
12.1



Breast Cancer 064006
16.8



Breast Cancer 9100266
13.9



Breast Margin 9100265
51.4



Breast Cancer A209073
13.8



Breast Margin A2090734
42.0



Breast cancer (OD06083)
26.6



Breast cancer node metastasis (OD06083)
39.5



Normal Liver
16.4



Liver Cancer 1026
11.6



Liver Cancer 1025
13.4



Liver Cancer 6004-T
11.3



Liver Tissue 6004-N
11.8



Liver Cancer 6005-T
28.5



Liver Tissue 6005-N
23.2



Liver Cancer 064003
4.3



Normal Bladder
12.3



Bladder Cancer 1023
16.5



Bladder Cancer A302173
8.9



Normal Stomach
47.0



Gastric Cancer 9060397
5.8



Stomach Margin 9060396
17.6



Gastric Cancer 9060395
19.1



Stomach Margin 9060394
50.0



Gastric Cancer 064005
8.4









Column A - Rel. Exp. (%) Ag1402, Run 173860059














TABLE IF










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
1.9



TE671- Medulloblastoma
8.0



D283 Med- Medulloblastoma
13.0



PFSK-1- Primitive Neuroectodermal
61.1



XF-498- CNS
16.0



SNB-78- Glioma
81.8



SF-268- Glioblastoma
4.5



T98G- Glioblastoma
16.3



SK-N-SH- Neuroblastoma (metastasis)
39.2



SF-295- Glioblastoma
5.3



Cerebellum
7.7



Cerebellum
13.4



NCI-H292- Mucoepidermoid lung carcinoma
2.1



DMS-114- Small cell lung cancer
31.2



DMS-79- Small cell lung cancer
31.9



NCI-H146- Small cell lung cancer
3.1



NCI-H526- Small cell lung cancer
9.5



NCI-N417- Small cell lung cancer
0.2



NCI-H82- Small cell lung cancer
3.7



NCI-H157- Squamous cell lung cancer (metastasis)
1.4



NCI-H1155- Large cell lung cancer
9.2



NCI-H1299- Large cell lung cancer
1.5



NCI-H727- Lung carcinoid
1.5



NCI-UMC-11- Lung carcinoid
2.0



LX-1- Small cell lung cancer
1.5



Colo-205- Colon cancer
0.9



KM12- Colon cancer
0.9



KM20L2- Colon cancer
0.5



NCI-H716- Colon cancer
5.6



SW-48- Colon adenocarcinoma
0.3



SW1116- Colon adenocarcinoma
5.3



LS 174T- Colon adenocarcinoma
1.1



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
0.4



NCI-SNU-5- Gastric carcinoma
0.8



KATO III- Gastric carcinoma
2.4



NCI-SNU-16- Gastric carcinoma
0.2



NCI-SNU-1- Gastric carcinoma
1.6



RF-1- Gastric adenocarcinoma
0.5



RF-48- Gastric adenocarcinoma
0.9



MKN-45- Gastric carcinoma
0.7



NCI-N87- Gastric carcinoma
0.9



OVCAR-5- Ovarian carcinoma
1.7



RL95-2- Uterine carcinoma
6.7



HelaS3- Cervical adenocarcinoma
3.3



Ca Ski- Cervical epidermoid carcinoma (metastasis)
1.9



ES-2- Ovarian clear cell carcinoma
3.2



Ramos- Stimulated with PMA/ionomycin 6 h
0.8



Ramos- Stimulated with PMA/ionomycin 14 h
0.6



MEG-01- Chronic myelogenous leukemia
1.0



(megokaryoblast)



Raji- Burkitt's lymphoma
0.1



Daudi- Burkitt's lymphoma
1.0



U266- B-cell plasmacytoma
1.0



CA46- Burkitt's lymphoma
2.0



RL- non-Hodgkin's B-cell lymphoma
0.2



JM1- pre-B-cell lymphoma
2.6



Jurkat- T cell leukemia
5.1



TF-1- Erythroleukemia
1.0



HUT 78- T-cell lymphoma
3.6



U937- Histiocytic lymphoma
100.0



KU-812- Myelogenous leukemia
1.5



769-P- Clear cell renal carcinoma
0.7



Caki-2- Clear cell renal carcinoma
0.9



SW 839- Clear cell renal carcinoma
0.5



Rhabdoid kidney tumor
34.9



Hs766T- Pancreatic carcinoma (LN metastasis)
0.9



CAPAN-1- Pancreatic adenocarcinoma (liver
5.5



metastasis)



SU86.86- Pancreatic carcinoma (liver metastasis)
1.7



BxPC-3- Pancreatic adenocarcinoma
0.8



HPAC- Pancreatic adenocarcinoma
0.2



MIA PaCa-2- Pancreatic carcinoma
0.0



CFPAC-1- Pancreatic ductal adenocarcinoma
2.1



PANC-1- Pancreatic epithelioid ductal carcinoma
0.7



T24- Bladder carcinma (transitional cell)
1.1



5637- Bladder carcinoma
0.9



HT-1197- Bladder carcinoma
0.6



UM-UC-3- Bladder carcinma (transitional cell)
0.6



A204- Rhabdomyosarcoma
12.3



HT-1080- Fibrosarcoma
7.3



MG-63- Osteosarcoma
11.9



SK-LMS-1- Leiomyosarcoma (vulva)
5.0



SJRH30- Rhabdomyosarcoma (met to bone marrow)
2.3



A431- Epidermoid carcinoma
1.3



WM266-4- Melanoma
8.7



DU 145- Prostate carcinoma (brain metastasis)
0.0



MDA-MB-468- Breast adenocarcinoma
1.8



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.3



SCC-15- Squamous cell carcinoma of tongue
0.1



CAL 27- Squamous cell carcinoma of tongue
0.7









Column A - Rel. Exp. (%) Ag1402, Run 182113343














TABLE IG










Panel 4D









Tissue Name
A
B












Secondary Th1 act
0.5
1.0


Secondary Th2 act
0.2
0.4


Secondary Tr1 act
0.9
0.6


Secondary Th1 rest
0.3
0.1


Secondary Th2 rest
0.4
0.2


Secondary Tr1 rest
0.3
0.2


Primary Th1 act
1.1
0.8


Primary Th2 act
0.9
0.8


Primary Tr1 act
1.9
1.4


Primary Th1 rest
1.6
1.1


Primary Th2 rest
0.7
0.5


Primary Tr1 rest
1.6
1.2


CD45RA CD4 lymphocyte act
16.7
13.4


CD45RO CD4 lymphocyte act
0.5
0.3


CD8 lymphocyte act
0.4
0.3


Secondary CD8 lymphocyte rest
0.5
0.4


Secondary CD8 lymphocyte act
0.6
0.3


CD4 lymphocyte none
0.5
0.4


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.6
0.3


LAK cells rest
0.4
0.4


LAK cells IL-2
0.5
0.4


LAK cells IL-2 + IL-12
0.7
0.5


LAK cells IL-2 + IFN gamma
0.9
0.5


LAK cells IL-2 + IL-18
0.5
0.3


LAK cells PMA/ionomycin
0.1
0.1


NK Cells IL-2 rest
0.6
0.5


Two Way MLR 3 day
0.5
0.3


Two Way MLR 5 day
0.4
0.2


Two Way MLR 7 day
0.3
0.3


PBMC rest
0.9
0.6


PBMC PWM
1.5
1.2


PBMC PHA-L
2.1
1.5


Ramos (B cell) none
0.6
1.0


Ramos (B cell) ionomycin
1.1
0.9


B lymphocytes PWM
1.0
0.7


B lymphocytes CD40L and IL-4
0.5
0.3


EOL-1 dbcAMP
7.0
6.6


EOL-1 dbcAMP PMA/ionomycin
1.0
0.7


Dendritic cells none
1.0
0.5


Dendritic cells LPS
0.1
0.1


Dendritic cells anti-CD40
0.5
0.9


Monocytes rest
3.7
2.5


Monocytes LPS
4.4
3.7


Macrophages rest
0.2
0.3


Macrophages LPS
0.3
0.2


HUVEC none
7.6
5.5


HUVEC starved
13.4
12.4


HUVEC IL-1beta
2.6
2.2


HUVEC IFN gamma
20.3
18.2


HUVEC TNF alpha + IFN gamma
1.5
1.1


HUVEC TNF alpha + IL4
2.4
2.4


HUVEC IL-11
14.0
12.5


Lung Microvascular EC none
14.0
12.0


Lung Microvascular EC TNFalpha + IL-1beta
7.0
7.2


Microvascular Dermal EC none
39.2
40.1


Microsvasular Dermal EC TNFalpha + IL-1beta
14.4
12.4


Bronchial epithelium TNFalpha + IL1beta
0.5
0.5


Small airway epithelium none
2.1
1.5


Small airway epithelium TNFalpha + IL-1beta
0.2
0.3


Coronery artery SMC rest
18.7
17.9


Coronery artery SMC TNFalpha + IL-1beta
17.4
13.6


Astrocytes rest
0.5
0.3


Astrocytes TNFalpha + IL-1beta
0.6
0.6


KU-812 (Basophil) rest
0.6
0.4


KU-812 (Basophil) PMA/ionomycin
1.0
0.6


CCD1106 (Keratinocytes) none
0.1
0.2


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.6
0.2


Liver cirrhosis
4.9
5.7


Lupus kidney
5.3
4.9


NCI-H292 none
0.6
0.7


NCI-H292 IL-4
0.4
0.7


NCI-H292 IL-9
1.1
0.7


NCI-H292 IL-13
0.6
0.5


NCI-H292 IFN gamma
0.7
0.5


HPAEC none
12.0
11.7


HPAEC TNF alpha + IL-1 beta
6.2
5.1


Lung fibroblast none
64.6
66.0


Lung fibroblast TNF alpha + IL-1 beta
26.6
24.7


Lung fibroblast IL-4
64.6
66.9


Lung fibroblast IL-9
51.4
42.9


Lung fibroblast IL-13
100.0
100.0


Lung fibroblast IFN gamma
84.1
82.4


Dermal fibroblast CCD1070 rest
71.2
60.7


Dermal fibroblast CCD1070 TNF alpha
41.8
39.0


Dermal fibroblast CCD1070 IL-1 beta
45.7
43.8


Dermal fibroblast IFN gamma
25.3
19.5


Dermal fibroblast IL-4
45.7
42.0


IBD Colitis 2
1.2
1.1


IBD Crohn's
2.0
1.6


Colon
8.4
8.2


Lung
29.9
23.7


Thymus
11.3
10.8


Kidney
7.1
5.2







Column A - Rel. Exp. (%) Ag1402, Run 139531667





Column B - Rel. Exp. (%) Ag1402, Run 145633378














TABLE IH










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
32.5



97476_Patient-07sk_skeletal muscle
23.2



97477_Patient-07ut_uterus
27.0



97478_Patient-07pl_placenta
62.9



99167_Bayer Patient 1
6.9



97482_Patient-08ut_uterus
45.1



97483_Patient-08pl_placenta
55.9



97486_Patient-09sk_skeletal muscle
6.4



97487_Patient-09ut_uterus
68.8



97488_Patient-09pl_placenta
42.0



97492_Patient-10ut_uterus
100.0



97493_Patient-10pl_placenta
51.4



97495_Patient-11go_adipose
21.2



97496_Patient-11sk_skeletal muscle
11.7



97497_Patient-11ut_uterus
61.1



97498_Patient-11pl_placenta
22.7



97500_Patient-12go_adipose
40.6



97501_Patient-12sk_skeletal muscle
22.2



97502_Patient-12ut_uterus
73.2



97503_Patient-12pl_placenta
22.8



94721_Donor 2 U - A_MesenchymalStem Cells
51.8



94722_Donor 2 U - B_Mesenchymal Stem Cells
40.9



94723_Donor 2 U - C_Mesenchymal Stem Cells
37.9



94709_Donor 2 AM - A_adipose
35.8



94710_Donor 2 AM - B_adipose
23.3



94711_Donor 2 AM - C_adipose
17.1



94712_Donor 2 AD - A_adipose
49.3



94713_Donor 2 AD - B_adipose
69.3



94714_Donor 2 AD - C_adipose
66.9



94742_Donor 3 U - A_Mesenchymal Stem Cells
17.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
23.5



94730_Donor 3 AM - A_adipose
26.2



94731_Donor 3 AM - B_adipose
16.2



94732_Donor 3 AM - C_adipose
19.9



94733_Donor 3 AD - A_adipose
34.4



94734_Donor 3 AD - B_adipose
25.9



94735_Donor 3 AD - C_adipose
34.2



77138_Liver_HepG2untreated
19.5



73556_Heart_Cardiac stromal cells (primary)
25.2



81735_Small Intestine
20.6



72409_Kidney_Proximal Convoluted Tubule
0.6



82685_Small intestine_Duodenum
5.3



90650_Adrenal_Adrenocortical adenoma
0.2



72410_Kidney_HRCE
0.7



72411_Kidney_HRE
1.8



73139_Uterus_Uterine smooth muscle cells
45.1









Column A - Rel. Exp. (%) Ag1402, Run 258861208







HASS Panel v1.0 Summary: Ag1402 Highest expression of this gene was detected in primary melanocytes A5 sample (CT=27). This gene showed wide spread expression in this panel with significant expression in astrocytes, glioma and the cancer cell lines subjected to serum starvation, acidosis and anoxia for different time periods. This expression pattern demonstrates that this gene or its protein product is important for cell survival and proliferation.


Human Metabolic Summary: Ag1402 Highest expression of this gene was detected in histiocystic lymphoma cell line(CT=23.6). This gene showed a ubiquitous expression with high expression in cancer cell lines, and metabolic tissues. Please see panel 1.2 for further discussion on the utility of this gene.


Panel 1.2 Summary: Ag1402 This gene was expressed at highest levels in heart (CT=22-23), prostate, and ovarian tissue and was expressed to a lesser degree across a number of samples in Panel 1.2. This gene was expressed largely in normal tissues when compared to cultured cell lines. Expression of this gene is useful for differentiating between heart, prostate and ovarian tissue from other tissues.


Among other metabolically relevant tissues, this gene was moderately expressed in thyroid, pituitary gland, and pancreas, and was highly expressed in adrenal gland, skeletal muscle and fetal/adult liver. High expression of this gene in insulin-responsive tissues such as skeletal muscle and liver demonstrates that this gene or gene product is an effective drug target for the treatment of Type 2 diabetes. High expression in adrenal gland and heart also shows that this gene or its protein product is an antibody, protein, or small molecule target for the treatment of diseases involving these two tissues.


This gene encodes a putative leucine-rich-repeat (LRR), GPCR-like protein. In Drosophilia, the LRR region of axon guidance proteins has been shown to be critical for function, especially in axon repulsion (Battye R. J. Neurosci. 21: 4290-4298.). The leucine-rich-repeat protein encoded by this gene showed high expression across all brain regions, with expression detected in amygdala, cerebellum, hippocampus, thalamus, cerebral cortex and spinal cord, making it an excellent candidate neuronal guidance protein for axons, dendrites and/or growth cones in general. Therapeutic modulation of the levels of this gene or protein, or possible signaling via this protein, is of utility in enhancing/directing compensatory synaptogenesis and fiber growth in the CNS in response to neuronal death (stroke, head trauma), axon lesion (spinal cord injury), or neurodegeneration (Alzheimer's, Parkinson's, Huntington's, vascular dementia or any neurodegenerative disease). In addition, the this gene showed very high levels of expression in the substantia nigra, where loss of dopaminergic neurons is a hallmark of Parkinson's disease and progressive supranuclear palsy. Therapies based upon this gene or its protein product are useful in the treatment of these diseases.


This protein also contains homology to the GPCR family of receptors. Several neurotransmitter receptors are GPCRs, including the dopamine receptor family, the serotonin receptor family, the GABAB receptor, muscarinic acetylcholine receptors, and others; thus this GPCR represents a novel neurotransmitter receptor. Targeting various neurotransmitter receptors (dopamine, serotonin) has proven to be an effective therapy in psychiatric illnesses such as schizophrenia, bipolar disorder and depression. Furthermore the cerebral cortex and hippocampus are regions of the brain that are known to play critical roles in Alzheimer's disease, seizure disorders, and in the normal process of memory formation. Therapeutic modulation of this gene or its protein product is beneficial in one or more of these diseases, as is stimulation and/or blockade of the receptor coded for by the gene. Levels of this gene were high, however, in areas outside of the central nervous system (such as the heart, ovaries, and prostate), demonstrating the possibility of a wider role in intercellular signaling.


Panel 2.2 Summary: Ag1402 Among the samples on this panel, expression of this gene was highest in a sample of normal ovarian tissue adjacent to an ovarian cancer (CT=28.5). There was also a predominance of this expression pattern in a number of other matched normal tissue samples including lung, colon and kidney. Umatched normal tissue samples, such as breast, uterus, ovary and prostate also showed appreciable expression of this gene. This gene or its protein product are useful in differentiating adjacent normal tissue from corresponding malignant tissue for the above listed tissue types. The therapeutic modulation of the gene or the protein encoded by this gene is of use in the treatment of the above listed malignancies.


Panel 3D Summary: : Ag1402 Expression of this gene was highest in a sample derived from a histiocytic lymphoma cell line (CT=28.2). This gene was also highly expressed in several CNS cancer cell lines and cell lines derived from a variety of sarcomas. The expression of this gene is useful for the differentiation of cell lines derived from sarcomas and CNS cancers vs. other cell lines. Therapeutic modulation of this gene or gene product is of therapeutic benefit in the treatment of sarcomas or CNS cancers.


Panel 4D Summary: This gene was highly expressed in endothelium and fibroblasts and was selectively down regulated in these tissues by treatment with IL-1beta and TNFalpha. This gene encodes a putative LRR-containing GPCR that is down regulated in response to the proinflammatory cytokines IL-1beta and TNFalpha in endothelium and fibroblasts. The natural ligand for this GPCR is induced by treatment with TNF and IL-1 and subsequent ligand binding to this putative GPCR results in feedback inhibition. Other signaling pathways induced by TNF and IL-1 reduce the level of transcription of this gene. Antibodies, small molecule or protein therapeutics which prevent the signaling through this putative GPCR are important in the treatment of diseases such as asthma, emphysema, psoriasis, and arthritis.


Panel 5 Islet Summary: Ag1402 Highest expression of this gene was detected in the uterus of a diabetic patient on insulin (CT=28). This gene showed widespread expression in this panel. This pattern was in agreement with the expression profile in General_screening_panel_v1.2 and demonstrates a role for the gene product in cell survival and proliferation. Please see panel 1.2 for further discussion on the utility of this gene.


J. CG51027-06: Optoid Binding Protein/Cell Adhesion Molecule Precursor


Expression of full-length physical clone CG51027-06 was assessed using the primer-probe set Ag6959, described in Table JA. Results of the RTQ-PCR runs are shown in Table JB.

TABLE JAProbe Name Ag6959StartSEQ IDPrimersSequencesLengthPositionNoForward5′-gtctacctcattgtccacgt-3′204201294ProbeTET-5′-ctcgtcgcctgtgatggtgaatga-3′-244611295TAMRAReverse5′-caaagcaggttcacattgc-3′194901296









TABLE JB










General_screening_panel_v1.6










Tissue Name
A














Adipose
0.2



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.1



Melanoma* M14
0.1



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.3



Squamous cell carcinoma SCC-4
0.0



Testis Pool
5.8



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.2



Placenta
0.0



Uterus Pool
0.0



Ovarian ca. OVCAR-3
0.2



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.4



Ovary
4.5



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.1



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.3



Trachea
0.1



Lung
0.0



Fetal Lung
1.6



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
1.3



Lung ca. A549
0.0



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
25.0



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.1



Lung ca. NCI-H522
21.5



Liver
0.3



Fetal Liver
1.0



Liver ca. HepG2
0.0



Kidney Pool
0.1



Fetal Kidney
2.3



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.1



Renal ca. TK-10
0.0



Bladder
0.2



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
0.6



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.2



Small Intestine Pool
0.2



Stomach Pool
0.2



Bone Marrow Pool
0.0



Fetal Heart
0.2



Heart Pool
0.0



Lymph Node Pool
0.0



Fetal Skeletal Muscle
0.0



Skeletal Muscle Pool
0.0



Spleen Pool
0.2



Thymus Pool
0.0



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.0



CNS cancer (neuro; met) SK-N-AS
3.8



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.1



Brain (Amygdala) Pool
9.3



Brain (cerebellum)
100.0



Brain (fetal)
61.1



Brain (Hippocampus) Pool
9.2



Cerebral Cortex Pool
25.9



Brain (Substantia nigra) Pool
18.0



Brain (Thalamus) Pool
16.3



Brain (whole)
22.8



Spinal Cord Pool
18.3



Adrenal Gland
0.9



Pituitary gland Pool
0.6



Salivary Gland
0.0



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
0.0









Column A - Rel. Exp. (%) Ag6959, Run 278388920







General_screeningpanel_v1.6 Summary: Ag6959 Highest expression of this gene was detected in cerebellum (CT=28.6). This gene showed a highly brain-preferential expression, and was expressed at moderate levels in all brain regions examined. This gene codes for a homolog of rat opioid-binding cell adhesion molecule (OBCAM), which is involved in axonal outgrowth (Hachisuka A, Brain Res Dev Brain Res 122(2):183-91) and may be involved in the synaptic machinery. The limited ability of the CNS to regenerate after injury is the major impediment to the treatment of stroke, spinal cord and head trauma, and neurodegenerative diseases. Modulation of this gene, expressed protein and/or use of antibodies or small molecule drug targeting this gene or gene product is useful in enhancing neuroregeneration in any or all of these clinical conditions.


Moderate expression of this gene was also seen in two lung cancer cell line. Expression of this gene is useful as marker to detect the presence of lung cancer and modulation of this gene or expressed protein is useful in the treatment of lung cancer.


K. CG51027-09: Optiod Bindin Protein/Cell Adhesion Molecule Precursor


Expression of gene CG51027-09 was assessed using the primer-probe sets Ag3090 and Ag3092, described in Tables KA and KB. Results of the RTQ-PCR runs are shown in Tables KC and KD.

TABLE KAProbe Name Ag3090StartSEQ IDPrimersSequencesLengthPositionNoForward5′-atgacagactgctgagcagc-3′202411297ProbeTET-5′-aaggcctgaaggtgcagacggag-3′-232081298TAMRAReverse5′-cgttggcaaagagaagcat-3′191771299









TABLE KB










Probe Name Ag3092















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-atgacagactgctgagcagc-3′
20
241
1300






Probe
TET-5′-aaggcctgaaggtgcagacggag-3′-
23
208
1301



TAMRA





Reverse
5′-cgttggcaaagagaagcat-3′
19
177
1302
















TABLE KC










Panel 1.3D











Tissue Name
A
B















Liver adenocarcinoma
0.3
0.0



Pancreas
0.0
0.0



Pancreatic ca. CAPAN 2
0.0
0.0



Adrenal gland
1.5
0.0



Thyroid
0.0
0.0



Salivary gland
0.0
0.0



Pituitary gland
3.4
0.2



Brain (fetal)
100.0
53.6



Brain (whole)
29.5
51.8



Brain (amygdala)
31.9
51.1



Brain (cerebellum)
94.0
71.7



Brain (hippocampus)
41.2
91.4



Brain (substantia nigra)
27.0
24.7



Brain (thalamus)
20.3
100.0



Cerebral Cortex
89.5
37.4



Spinal cord
16.4
7.9



glio/astro U87-MG
0.0
0.0



glio/astro U-118-MG
0.0
0.0



astrocytoma SW1783
0.0
0.0



neuro*; met SK-N-AS
2.2
3.2



astrocytoma SF-539
0.0
0.0



astrocytoma SNB-75
1.4
0.0



glioma SNB-19
0.0
0.2



glioma U251
0.8
0.0



glioma SF-295
0.0
0.1



Heart (fetal)
0.0
0.1



Heart
0.0
0.0



Skeletal muscle (fetal)
0.0
5.2



Skeletal muscle
0.2
0.0



Bone marrow
0.0
0.0



Thymus
0.0
1.6



Spleen
0.3
1.6



Lymph node
0.0
0.0



Colorectal
1.7
0.1



Stomach
0.0
0.0



Small intestine
0.5
0.0



Colon ca. SW480
0.0
0.0



Colon ca.* SW620(SW480 met)
0.4
0.0



Colon ca. HT29
0.5
0.1



Colon ca. HCT-116
0.0
0.2



Colon ca. CaCo-2
0.5
0.0



Colon ca. tissue(ODO3866)
5.8
0.9



Colon ca. HCC-2998
0.7
1.0



Gastric ca.* (liver met) NCI-N87
0.3
0.0



Bladder
0.0
0.0



Trachea
0.0
0.0



Kidney
0.0
0.0



Kidney (fetal)
13.3
4.4



Renal ca. 786-0
0.2
0.0



Renal ca. A498
0.0
0.1



Renal ca. RXF 393
0.1
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.5
0.0



Liver
0.0
0.8



Liver (fetal)
1.2
0.0



Liver ca. (hepatoblast) HepG2
21.2
5.4



Lung
0.0
0.0



Lung (fetal)
1.8
2.1



Lung ca. (small cell) LX-1
0.0
0.0



Lung ca. (small cell) NCI-H69
0.2
0.0



Lung ca. (s. cell var.) SHP-77
1.6
0.0



Lung ca. (large cell)NCI-H460
0.0
2.0



Lung ca. (non-sm. cell) A549
0.8
0.0



Lung ca. (non-s. cell) NCI-H23
21.3
10.2



Lung ca. (non-s. cell) HOP-62
0.0
0.0



Lung ca. (non-s. cl) NCI-H522
27.0
3.7



Lung ca. (squam.) SW 900
0.0
0.1



Lung ca. (squam.) NCI-H596
0.0
0.0



Mammary gland
0.0
0.0



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.5
0.0



Breast ca.* (pl. ef) T47D
0.0
0.0



Breast ca. BT-549
0.0
0.1



Breast ca. MDA-N
0.4
0.0



Ovary
9.0
2.0



Ovarian ca. OVCAR-3
0.0
0.0



Ovarian ca. OVCAR-4
1.1
0.0



Ovarian ca. OVCAR-5
0.4
0.0



Ovarian ca. OVCAR-8
0.5
0.4



Ovarian ca. IGROV-1
0.0
0.1



Ovarian ca.* (ascites) SK-OV-3
0.0
2.6



Uterus
0.0
0.0



Placenta
0.0
0.0



Prostate
0.0
0.3



Prostate ca.* (bone met)PC-3
0.2
0.1



Testis
5.9
15.0



Melanoma Hs688(A).T
0.0
0.0



Melanoma* (met) Hs688(B).T
0.0
0.0



Melanoma UACC-62
0.0
0.1



Melanoma M14
0.0
0.1



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0



Adipose
3.1
0.8









Column A - Rel. Exp. (%) Ag3090, Run 167985244






Column B - Rel. Exp. (%) Ag3092, Run 165673593














TABLE KD










Panel 4D









Tissue Name
A
B












Secondary Th1 act
0.0
0.0


Secondary Th2 act
0.1
100.0


Secondary Tr1 act
0.1
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
0.0


Secondary Tr1 rest
0.0
0.0


Primary Th1 act
0.0
6.2


Primary Th2 act
0.0
0.0


Primary Tr1 act
0.0
0.0


Primary Th1 rest
0.0
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
2.9


CD45RO CD4 lymphocyte act
0.0
5.3


CD8 lymphocyte act
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
14.2


LAK cells rest
0.0
0.0


LAK cells IL-2
0.0
0.0


LAK cells IL-2 + IL-12
0.1
0.0


LAK cells IL-2 + IFN gamma
0.0
15.2


LAK cells IL-2 + IL-18
0.0
0.0


LAK cells PMA/ionomycin
100.0
3.6


NK Cells IL-2 rest
0.0
0.0


Two Way MLR 3 day
0.0
0.0


Two Way MLR 5 day
0.0
0.0


Two Way MLR 7 day
0.0
14.6


PBMC rest
0.0
4.2


PBMC PWM
0.3
14.2


PBMC PHA-L
0.5
37.1


Ramos (B cell) none
0.1
0.0


Ramos (B cell) ionomycin
0.0
0.0


B lymphocytes PWM
0.2
2.6


B lymphocytes CD40L and IL-4
0.0
0.0


EOL-1 dbcAMP
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.5
37.9


Dendritic cells none
0.0
0.0


Dendritic cells LPS
0.0
17.3


Dendritic cells anti-CD40
0.0
0.0


Monocytes rest
0.0
0.0


Monocytes LPS
0.0
0.0


Macrophages rest
0.0
0.0


Macrophages LPS
0.0
5.0


HUVEC none
0.0
0.0


HUVEC starved
0.0
0.0


HUVEC IL-1beta
0.0
0.0


HUVEC IFN gamma
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0


HUVEC IL-11
0.0
0.0


Lung Microvascular EC none
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
0.0
4.9


Microvascular Dermal EC none
0.0
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.0


Bronchial epithelium TNFalpha + IL1beta
0.0
0.0


Small airway epithelium none
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
0.0
0.0


Coronery artery SMC rest
0.1
24.7


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
0.0
0.0


Astrocytes TNFalpha + IL-1 beta
0.0
0.0


KU-812 (Basophil) rest
0.0
25.9


KU-812 (Basophil) PMA/ionomycin
0.1
23.3


CCD1106 (Keratinocytes) none
0.0
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0
0.0


Liver cirrhosis
0.6
25.3


Lupus kidney
0.0
0.0


NCI-H292 none
0.0
0.0


NCI-H292 IL-4
0.0
0.0


NCI-H292 IL-9
0.0
0.0


NCI-H292 IL-13
0.0
0.0


NCI-H292 IFN gamma
0.0
0.0


HPAEC none
0.0
0.0


HPAEC TNF alpha + IL-1 beta
0.0
2.2


Lung fibroblast none
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast IL-4
0.0
0.0


Lung fibroblast IL-9
0.0
0.0


Lung fibroblast IL-13
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.1
0.0


Dermal fibroblast IFN gamma
0.0
0.0


Dermal fibroblast IL-4
0.1
0.0


IBD Colitis 2
0.0
0.0


IBD Crohn's
0.0
0.0


Colon
0.5
52.1


Lung
0.3
58.2


Thymus
0.0
0.0


Kidney
0.0
0.0







Column A - Rel. Exp. (%) Ag3090, Run 164391872





Column B - Rel. Exp. (%) Ag3092, Run 164399701







Panel 1.3D Summary: Ag3090/Ag3092 Highest expression of this gene was detected in regions of the brain, specifically the thalamus and fetal brain (CTS=29-30). This gene showed a highly brain-preferential expression, and was expressed at high levels in all brain regions examined. The protein encoded by this gene is a homolog of opioid-binding cell adhesion molecule (OBCAM), which is involved in axonal outgrowth and in the synaptic machinery. The limited ability of the CNS to regenerate after injury is the major impediment to the treatment of stroke, spinal cord and head trauma, and neurodegenerative diseases. Selective modulation of this gene or its protein product is useful in enhancing neuroregeneration in any or all of these clinical conditions.


Panel 4D Sumnary: Ag3092 Expression of this gene was limited to a sample derived from secondary Th2 cells (CT=34.2). Therapeutic modulation of this gene or expressed protein is useful in reducing or eliminating the symptoms in patients with diseases related to the functions of Th2 cells, including asthma and allergies.


L. CG51373-08 and CG51373-10 and CG51373-11: Weakly Similar to Neural Cell Adhesion Molecule 1, Large Isoform Precursor


Expression of full-length physical clone CG51373-08 and variants CG51373-10 and CG51373-11 was assessed using the primer-probe sets Ag271 and Ag271b, described in Tables LA and LB. Results of the RTQ-PCR runs are shown in Tables LC, LD, LE, LF and LG.

TABLE LAProbe Name Ag271StartSEQ IDPrimersSequencesLengthPositionNoForward5′-acctggacatagggcgtgtct-3′216901303ProbeTET-5′-cgaagcatgaacgaagccatccctag-3′-266561304TAMRAReverse5′-tcgatggaagtctccttgcc-3′206351305










TABLE LB










Probe Name Ag271b
















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-caccgtgagccaactgcttat-3′
21
1722
1306






Probe
TET-5′-agacacgccctatgtccaggtccg-3′-
24
1690
1307



TAMRA





Reverse
5′-ttcgttcatgcttcggcaa-3′
19
1667
1308
















TABLE LC










General_screening_panel_v1.4











Tissue Name
A
B















Adipose
6.4
6.4



Melanoma* Hs688(A).T
29.7
28.1



Melanoma* Hs688(B).T
26.4
25.7



Melanoma* M14
49.0
48.0



Melanoma* LOXIMVI
28.7
31.2



Melanoma* SK-MEL-5
9.4
11.7



Squamous cell carcinoma SCC-4
5.0
5.4



Testis Pool
1.8
1.6



Prostate ca.* (bone met) PC-3
19.8
22.4



Prostate Pool
2.0
2.4



Placenta
4.5
7.7



Uterus Pool
2.3
2.7



Ovarian ca. OVCAR-3
45.1
54.3



Ovarian ca. SK-OV-3
88.9
87.1



Ovarian ca. OVCAR-4
15.9
17.7



Ovarian ca. OVCAR-5
29.9
29.1



Ovarian ca. IGROV-1
54.0
50.3



Ovarian ca. OVCAR-8
29.9
36.9



Ovary
11.0
10.4



Breast ca. MCF-7
0.0
0.0



Breast ca. MDA-MB-231
0.0
29.7



Breast ca. BT 549
88.3
100.0



Breast ca. T47D
53.2
60.3



Breast ca. MDA-N
13.6
17.2



Breast Pool
7.6
9.7



Trachea
3.5
4.7



Lung
1.1
1.8



Fetal Lung
14.0
18.7



Lung ca. NCI-N417
3.3
2.9



Lung ca. LX-1
1.5
2.2



Lung ca. NCI-H146
0.0
0.0



Lung ca. SHP-77
0.1
0.2



Lung ca. A549
8.0
9.7



Lung ca. NCI-H526
0.9
0.6



Lung ca. NCI-H23
9.0
10.4



Lung ca. NCI-H460
15.0
19.3



Lung ca. HOP-62
15.4
20.91



Lung ca. NCI-H522
1.8
2.0



Liver
0.2
0.2



Fetal Liver
1.8
2.4



Liver ca. HepG2
0.0
0.0



Kidney Pool
7.6
12.9



Fetal Kidney
10.2
13.8



Renal ca. 786-0
62.9
77.9



Renal ca. A498
21.8
22.4



Renal ca. ACHN
45.4
49.3



Renal ca. UO-31
37.6
32.1



Renal ca. TK-10
36.6
37.4



Bladder
4.0
6.9



Gastric ca. (liver met.) NCI-N87
1.8
2.7



Gastric ca. KATO III
0.0
0.0



Colon ca. SW-948
4.0
4.2



Colon ca. SW480
7.5
9.1



Colon ca.* (SW480 met) SW620
2.7
3.2



Colon ca. HT29
1.3
2.3



Colon ca. HCT-116
7.0
8.3



Colon ca. CaCo-2
13.2
17.6



Colon cancer tissue
6.3
6.7



Colon ca. SW1116
0.1
0.0



Colon ca. Colo-205
0.0
0.0



Colon ca. SW-48
0.5
0.5



Colon Pool
5.9
5.6



Small Intestine Pool
4.2
4.1



Stomach Pool
5.4
5.7



Bone Marrow Pool
3.0
3.6



Fetal Heart
1.7
1.7



Heart Pool
3.1
3.1



Lymph Node Pool
6.8
10.5



Fetal Skeletal Muscle
2.4
2.1



Skeletal Muscle Pool
1.6
1.4



Spleen Pool
2.9
3.5



Thymus Pool
5.6
5.8



CMS cancer (glio/astro) U87-MG
25.0
31.0



CNS cancer (glio/astro) U-118-MG
47.3
56.6



CNS cancer (neuro; met) SK-N-AS
18.8
23.2



CNS cancer (astro) SF-539
36.9
44.8



CNS cancer (astro) SNB-75
100.0
100.0



CNS cancer (glio) SNB-19
28.7
57.4



CNS cancer (glio) SF-295
64.6
79.6



Brain (Amygdala) Pool
0.4
0.3



Brain (cerebellum)
1.2
1.3



Brain (fetal)
6.9
9.0



Brain (Hippocampus) Pool
0.8
0.6



Cerebral Cortex Pool
0.5
0.5



Brain (Substantia nigra) Pool
0.6
0.8



Brain (Thalamus) Pool
0.9
0.8



Brain (whole)
1.0
1.1



Spinal Cord Pool
0.8
0.7



Adrenal Gland
6.5
5.7



Pituitary gland Pool
0.4
0.4



Salivary Gland
1.5
1.4



Thyroid (female)
1.8
1.8



Pancreatic ca. CAPAN2
24.8
23.2



Pancreas Pool
7.7
9.0









Column A - Rel. Exp. (%) Ag271, Run 216607738






Column B - Rel. Exp. (%) Ag271b, Run 216607756














TABLE LD










Panel 1












Tissue Name
A
B
C
















Endothelial cells
11.0
5.0
3.9



Endothelial cells (treated)
3.8
4.3
4.9



Pancreas
2.7
3.1
1.9



Pancreatic ca. CAPAN 2
38.2
24.0
19.8



Adrenal gland
14.1
8.2
12.7



Thyroid
8.6
6.1
4.5



Salivary gland
3.5
4.1
3.3



Pituitary gland
9.5
10.2
7.3



Brain (fetal)
7.4
9.0
5.3



Brain (whole)
0.8
3.7
1.3



Brain (amygdala)
0.1
1.7
1.7



Brain (cerebellum)
4.8
10.5
2.3



Brain (hippocampus)
0.2
1.2
1.4



Brain (substantia nigra)
0.9
3.5
2.1



Brain (thalamus)
0.7
2.7
3.2



Brain (hypothalamus)
0.5
3.4
3.3



Spinal cord
2.1
4.0
3.6



glio/astro U87-MG
30.8
24.0
27.4



glio/astro U-118-MG
28.5
24.8
20.0



astrocytoma SW1783
21.0
17.1
17.0



neuro*; met SK-N-AS
24.1
17.6
29.9



astrocytoma SF-539
35.6
27.4
41.2



astrocytoma SNB-75
56.3
65.1
32.8



glioma SNB-19
42.6
53.6
55.9



glioma U251
39.8
26.8
48.3



glioma SF-295
43.2
33.4
31.9



Heart
15.2
4.5
6.8



Skeletal muscle
0.1
1.9
4.6



Bone marrow
1.1
1.7
2.1



Thymus
28.1
18.9
10.2



Spleen
2.5
5.1
3.2



Lymph node
6.8
6.0
2.0



Colon (ascending)
3.4
3.2
2.6



Stomach
11.6
12.0
3.3



Small intestine
5.9
8.7
5.5



Colon ca. SW480
0.0
1.8
5.2



Colon ca.* SW620 (SW480 met)
1.5
2.4
3.3



Colon ca. HT29
0.1
1.7
3.2



Colon ca. HCT-116
13.0
10.4
8.9



Colon ca. CaCo-2
27.9
21.8
17.3



Colon ca. HCT-15
1.7
5.0
7.3



Colon ca. HCC-2998
0.4
1.2
2.7



Gastric ca.* (liver met) NCI-N87
1.1
3.1
3.5



Bladder
30.8
15.9
19.1



Trachea
7.2
7.0
4.5



Kidney
14.8
8.9
9.8



Kidney (fetal)
53.6
55.9
27.7



Renal ca. 786-0
94.6
96.6
70.2



Renal ca. A498
65.5
65.5
46.3



Renal ca. RXF 393
41.8
27.7
29.1



Renal ca. ACHN
66.9
65.1
51.4



Renal ca. UO-31
46.0
41.8
54.0



Renal ca. TK-10
57.0
56.6
52.9



Liver
2.0
3.3
4.4



Liver (fetal)
2.0
2.4
2.9



Liver ca. (hepatoblast) HepG2
0.0
0.0
0.0



Lung
27.9
8.0
5.0



Lung (fetal)
23.8
11.9
5.3



Lung ca. (small cell) LX-1
0.0
1.4
3.2



Lung ca. (small cell) NCI-H69
2.9
4.2
8.2



Lung ca. (s. cell var.) SHP-77
0.0
0.4
0.2



Lung ca. (large cell)NCI-H460
29.5
27.0
51.4



Lung ca. (non-sm. cell) A549
6.6
7.1
12.2



Lung ca. (non-s. cell) NCI-H23
6.6
7.1
17.2



Lung ca. (non-s. cell) HOP-62
19.1
15.8
40.9



Lung ca. (non-s. cl) NCI-H522
4.3
5.4
5.0



Lung ca. (squam.) SW 900
15.8
17.1
19.1



Lung ca. (squam.) NCI-H596
8.3
8.7
10.5



Mammary gland
47.6
45.1
28.9



Breast ca.* (pl. ef) MCF-7
0.0
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
17.1
15.1
18.4



Breast ca.* (pl. ef) T47D
3.1
5.3
6.4



Breast ca. BT-549
70.7
65.1
26.1



Breast ca. MDA-N
43.8
25.7
35.4



Ovary
54.0
39.5
27.9



Ovarian ca. OVCAR-3
32.8
32.3
55.5



Ovarian ca. OVCAR-4
39.2
33.0
46.0



Ovarian ca. OVCAR-5
49.3
35.6
46.7



Ovarian ca. OVCAR-8
39.5
20.0
57.4



Ovarian ca. IGROV-1
46.0
48.0
100.0



Ovarian ca. (ascites) SK-OV-3
53.2
47.6
68.8



Uterus
36.6
9.9
9.4



Placenta
18.2
23.8
11.7



Prostate
6.4
6.7
4.3



Prostate ca.* (bone met) PC-3
32.8
37.6
50.3



Testis
27.7
23.8
4.7



Melanoma Hs688(A).T
21.5
23.0
13.6



Melanoma* (met) Hs688(B).T
25.3
25.3
20.3



Melanoma UACC-62
28.1
23.0
58.2



Melanoma M14
34.6
36.1
22.2



Melanoma LOX IMVI
100.0
100.0
12.2



Melanoma* (met) SK-MEL-5
12.1
10.9
14.3



Melanoma SK-MEL-28
53.6
79.0
2.5









Column A - Rel. Exp. (%) Ag271, Run 87588589






Column B - Rel. Exp. (%) Ag271, Run 88706798






Column C - Rel. Exp. (%) Ag271b, Run 97805788














TABLE LE










Panel 2.2










Tissue Name
A














Normal Colon
13.7



Colon cancer (OD06064)
45.4



Colon Margin (OD06064)
6.7



Colon cancer (OD06159)
1.7



Colon Margin (OD06159)
7.0



Colon cancer (OD06297-04)
5.3



Colon Margin (OD06297-05)
7.4



CC Gr.2 ascend colon (ODO3921)
10.5



CC Margin (ODO3921)
13.3



Colon cancer metastasis (OD06104)
1.8



Lung Margin (OD06104)
3.0



Colon mets to lung (OD04451-01)
21.0



Lung Margin (OD04451-02)
10.0



Normal Prostate
2.0



Prostate Cancer (OD04410)
3.9



Prostate Margin (OD04410)
10.3



Normal Ovary
78.5



Ovarian cancer (OD06283-03)
14.3



Ovarian Margin (OD06283-07)
16.3



Ovarian Cancer 064008
41.8



Ovarian cancer (OD06145)
36.6



Ovarian Margin (OD06145)
33.7



Ovarian cancer (OD06455-03)
6.0



Ovarian Margin (OD06455-07)
18.7



Normal Lung
20.7



Invasive poor diff. lung adeno (ODO4945-01
3.1



Lung Margin (ODO4945-03)
10.6



Lung Malignant Cancer (OD03126)
5.8



Lung Margin (OD03126)
10.4



Lung Cancer (OD05014A)
6.2



Lung Margin (OD05014B)
11.5



Lung cancer (OD06081)
5.3



Lung Margin (OD06081)
4.1



Lung Cancer (OD04237-01)
3.2



Lung Margin (OD04237-02)
37.9



Ocular Melanoma Metastasis
18.8



Ocular Melanoma Margin (Liver)
8.3



Melanoma Metastasis
40.1



Melanoma Margin (Lung)
13.5



Normal Kidney
16.0



Kidney Ca, Nuclear grade 2 (OD04338)
57.0



Kidney Margin (OD04338)
12.9



Kidney Ca Nuclear grade 1/2 (OD04339)
73.2



Kidney Margin (OD04339)
14.2



Kidney Ca, Clear cell type (OD04340)
20.0



Kidney Margin (OD04340)
35.1



Kidney Ca, Nuclear grade 3 (OD04348)
25.0



Kidney Margin (OD04348)
100.0



Kidney malignant cancer (OD06204B)
6.1



Kidney normal adjacent tissue (OD06204E)
12.3



Kidney Cancer (OD04450-01)
68.8



Kidney Margin (OD04450-03)
24.0



Kidney Cancer 8120613
0.0



Kidney Margin 8120614
11.7



Kidney Cancer 9010320
15.5



Kidney Margin 9010321
16.6



Kidney Cancer 8120607
70.7



Kidney Margin 8120608
11.4



Normal Uterus
36.6



Uterine Cancer 064011
11.7



Normal Thyroid
1.6



Thyroid Cancer 064010
10.2



Thyroid Cancer A302152
9.3



Thyroid Margin A302153
4.9



Normal Breast
31.4



Breast Cancer (OD04566)
3.1



Breast Cancer 1024
0.0



Breast Cancer (OD04590-01)
10.2



Breast Cancer Mets (OD04590-03)
19.1



Breast Cancer Metastasis (OD04655-05)
6.0



Breast Cancer 064006
14.2



Breast Cancer 9100266
12.5



Breast Margin 9100265
11.4



Breast Cancer A209073
15.7



Breast Margin A2090734
48.6



Breast cancer (OD06083)
14.3



Breast cancer node metastasis (OD06083)
15.6



Normal Liver
4.3



Liver Cancer 1026
12.3



Liver Cancer 1025
12.5



Liver Cancer 6004-T
12.4



Liver Tissue 6004-N
3.0



Liver Cancer 6005-T
21.0



Liver Tissue 6005-N
26.2



Liver Cancer 064003
2.5



Normal Bladder
9.7



Bladder Cancer 1023
8.4



Bladder Cancer A302173
11.0



Normal Stomach
22.8



Gastric Cancer 9060397
9.9



Stomach Margin 9060396
6.6



Gastric Cancer 9060395
11.8



Stomach Margin 9060394
23.7



Gastric Cancer 064005
6.1









Column A - Rel. Axp. (%) Ag271b, Run 175148876














TABLE LF










Panel 3D








Tissue Name
A











Daoy- Medulloblastoma
9.2


TE671- Medulloblastoma
3.3


D283 Med- Medulloblastoma
32.1


PFSK-1- Primitive Neuroectodermal
9.4


XF-498- CNS
42.3


SNB-78- Glioma
22.2


SF-268- Glioblastoma
17.2


T98G- Glioblastoma
32.3


SK-N-SH- Neuroblastoma (metastasis)
20.6


SF-295- Glioblastoma
12.8


Cerebellum
1.1


Cerebellum
1.2


NCI-H292- Mucoepidermoid lung carcinoma
12.6


DMS-114- Small cell lung cancer
4.1


DMS-79- Small cell lung cancer
0.3


NCI-H146- Small cell lung cancer
0.0


NCI-H526- Small cell lung cancer
2.8


NCI-N417- Small cell lung cancer
4.3


NCI-H82- Small cell lung cancer
6.5


NCI-H157- Squamous cell lung cancer (metastasis)
19.6


NCI-H1155- Large cell lung cancer
2.0


NCI-H1299- Large cell lung cancer
12.5


NCI-H727- Lung carcinoid
3.0


NCI-UMC-11- Lung carcinoid
0.0


LX-1- Small cell lung cancer
1.4


Colo-205- Colon cancer
0.0


KM12- Colon cancer
0.0


KM20L2- Colon cancer
1.5


NCI-H716- Colon cancer
0.0


SW-48- Colon adenocarcinoma
1.3


SW1116- Colon adenocarcinoma
0.0


LS 174T- Colon adenocarcinoma
0.0


SW-948- Colon adenocarcinoma
1.0


SW-480- Colon adenocarcinoma
0.4


NCI-SNU-5- Gastric carcinoma
4.5


KATO III- Gastric carcinoma
100.0


NCI-SNU-16- Gastric carcinoma
15.1


NCI-SNU-1- Gastric carcinoma
0.0


RF-1- Gastric adenocarcinoma
0.0


RF-48- Gastric adenocarcinoma
0.0


MKN-45- Gastric carcinoma
2.5


NCI-N87- Gastric carcinoma
1.9


OVCAR-5- Ovarian carcinoma
2.3


RL95-2- Uterine carcinoma
6.0


HelaS3- Cervical adenocarcinoma
4.0


Ca Ski- Cervical epidermoid carcinoma (metastasis)
11.9


ES-2- Ovarian clear cell carcinoma
8.8


Ramos- Stimulated with PMA/ionomycin 6 h
0.0


Ramos- Stimulated with PMA/ionomycin 14 h
0.0


MEG-01- Chronic myelogenous leukemia (megokaryoblast)
3.8


Raji- Burkitt's lymphoma
0.0


Daudi- Burkitt's lymphoma
0.0


U266- B-cell plasmacytoma
0.0


CA46- Burkitt's lymphoma
0.0


RL- non-Hodgkin's B-cell lymphoma
0.0


JM1- pre-B-cell lymphoma
0.0


Jurkat- T cell leukemia
5.6


TF-1- Erythroleukemia
0.0


HUT 78- T-cell lymphoma
0.3


U937- Histiocytic lymphoma
0.0


KU-812- Myelogenous leukemia
0.2


769-P- Clear cell renal carcinoma
42.3


Caki-2- Clear cell renal carcinoma
26.6


SW 839- Clear cell renal carcinoma
30.1


Rhabdoid kidney tumor
11.5


Hs766T- Pancreatic carcinoma (LN metastasis)
23.7


CAPAN-1- Pancreatic adenocarcinoma (liver metastasis)
3.8


SU86.86- Pancreatic carcinoma (liver metastasis)
10.7


BxPC-3- Pancreatic adenocarcinoma
6.0


HPAC- Pancreatic adenocarcinoma
2.4


MIA PaCa-2- Pancreatic carcinoma
5.1


CFPAC-1- Pancreatic ductal adenocarcinoma:
26.1


PANC-1- Pancreatic epithelioid ductal carcinoma
21.2


T24- Bladder carcinma (transitional cell)
15.2


5637- Bladder carcinoma
6.3


HT-1197- Bladder carcinoma
1.0


UM-UC-3- Bladder carcinma (transitional cell)
3.7


A204- Rhabdomyosarcoma
6.7


HT-1080- Fibrosarcoma
25.5


MG-63- Osteosarcoma
16.5


SK-LMS-1- Leiomyosarcoma (vulva)
11.2


SJRH30- Rhabdomyosarcoma (met to bone marrow)
4.7


A431- Epidermoid carcinoma
4.5


WM266-4- Melanoma
22.1


DU 145- Prostate carcinoma (brain metastasis)
1.6


MDA-MB-468- Breast adenocarcinoma
10.6


SCC-4- Squamous cell carcinoma of tongue
0.7


SCC-9- Squamous cell carcinoma of tongue
0.3


SCC-15- Squamous cell carcinoma of tongue
0.3


CAL 27- Squamous cell carcinoma of tongue
10.4







Column A - Rel. Exp. (%) Ag271b, Run 182114260














TABLE LG










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.4



Secondary Tr1 act
0.3



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.6



Primary Th1 rest
0.3



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
33.9



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.1



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.1



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.1



Two Way MLR 7 day
0.3



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.3



EOL-1 dbcAMP
0.3



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.6



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
18.2



HUVEC starved
27.0



HUVEC IL-1 beta
21.0



HUVEC IFN gamma
19.2



HUVEC TNF alpha + IFN gamma
26.6



HUVEC TNF alpha + IL4
32.5



HUVEC IL-11
11.8



Lung Microvascular EC none
43.2



Lung Microvascular EC TNFalpha + IL-1beta
34.4



Microvascular Dermal EC none
35.8



Microsvasular Dermal EC TNFalpha + IL-1beta
26.4



Bronchial epithelium TNFalpha + IL1beta
32.3



Small airway epithelium none
16.8



Small airway epithelium TNFalpha + IL-1beta
33.2



Coronery artery SMC rest
47.3



Coronery artery SMC TNFalpha + IL-1beta
51.8



Astrocytes rest
59.0



Astrocytes TNFalpha + IL-1beta
38.7



KU-812 (Basophil) rest
0.6



KU-812 (Basophil) PMA/ionomycin
1.0



CCD1106 (Keratinocytes) none
36.1



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
30.6



Liver cirrhosis
4.4



NCI-H292 none
12.7



NCI-H292 IL-4
17.3



NCI-H292 IL-9
17.3



NCI-H292 IL-13
17.9



NCI-H292 IFN gamma
12.8



HPAEC none
17.0



HPAEC TNF alpha + IL-1 beta
39.8



Lung fibroblast none
71.7



Lung fibroblast TNF alpha + IL-1 beta
55.5



Lung fibroblast IL-4
66.4



Lung fibroblast IL-9
100.0



Lung fibroblast IL-13
52.1



Lung fibroblast IFN gamma
70.7



Dermal fibroblast CCD1070 rest
66.0



Dermal fibroblast CCD1070 TNF alpha
61.1



Dermal fibroblast CCD1070 IL-1 beta
61.6



Dermal fibroblast IFN gamma
43.2



Dermal fibroblast IL-4
59.0



Dermal Fibroblasts rest
36.6



Neutrophils TNFa + LPS
2.0



Neutrophils rest
6.7



Colon
4.4



Lung
20.4



Thymus
11.6



Kidney
26.6









Column A - Rel. Exp. (%) Ag271b, Run 174261189







General_screeningpanel_v1.4 Summary: Ag271/271b Highest expression of this gene was detected in a sample derived from a brain cancer cell line (SNB-75) (CTs=24). There was substantial expression in a number of brain cancer cell lines, renal cancer cell lines, breast cancer cell lines, ovarian cancer cell lines and melanoma cell lines. The expression of this gene is useful for the differentiation of SNB-75 cells from the other samples in the panel. Therapeutic modulation of this gene or gene product, through the use of small molecule drugs, antibodies or protein therapeutics is beneficial for the treatment of brain, renal, ovarian, breast cancers or melanoma.


This gene also had moderate and widespread expression in metabolic tissues including adipose, adult and fetal heart, adult and fetal skeletal muscle, adrenal, thyroid and pancreas. This gene or gene product is an antibody, protein or small molecule target for the treatment of metabolic and endocrine disease, including obesity and Types 1 and 2 diabetes. This gene was differentially expressed in fetal (CTs=29-30) vs adult liver (CTs=33) and expression of this gene or its protein product is useful differentiate between the two sources of this tissue. The higher levels of expression in fetal liver demonstrate that this gene product is involved in the development of the liver and that this gene or gene product is useful in restoring mass or function to the adult liver.


This panel also showed low but significant levels of expression of this gene in the CNS. Please see panel 1 for a discussion of utility of this gene in the central nervous system.


Panel 1 Summary: Ag271/Ag271b Highest expression of this gene was detected in a number of cancer cell lines, including ovarian cancer, pancreatic cancer, prostate cancer, melanoma, renal carcinoma, and CNS cancers, when compared to normal controls. The data presented in panel 1 showed that this gene was expressed highly in samples derived from cancer cell lines and not highly expressed in samples derived from normal tissues. Within the normal kidney samples there was a consistent difference between adult and fetal tissues. Since cell lines and fetal tissues are more proliferative than normal tissues, this expression profile demonstrates that this gene or its protein product is involved in cell proliferation. Inhibition of expression or function of this gene or gene product is a therapeutic avenue for the treatment of cancer or other disease that involve cell proliferation. Therapeutic targeting of this gene or gene product with a monoclonal antibody, protein therapeutic or small molecule will limit or block the extent of tumor cell migration and invasion and tumor metastasis, particularly in melanomas, prostate cancers, pancreatic cancers, ovarian cancers, renal cell carcinomas and CNS cancers. This gene is also be an effective marker for the diagnosis and detection of a variety of cancers.


This gene was also expressed in all CNS tissues examined, but at lower levels than the relatively high expression in all cancer cell lines examined and fetal kidney. This further demonstrates that this gene or its gene product is involved in the cell cycle or cell proliferation because the majority of cells in the CNS are post-mitotic. This gene is useful in stem cell research or therapy in controlling the transition from stem cell to post-mitotic differentiated cell and is of benefit in any disease associated with neuron loss such as Alzheimer's, Parkinson's, or Huntington's diseases, stroke, head trauma, spinal cord trauma, or spinocerebellar ataxia.


Panel 2.2 Summary: Ag271b Highest expression of this gene was seen in normal kidney. Significant expression was also seen in kidney cancer, melanoma and colon cancer when compared to normal adjacent tissue. Expression of this gene is useful in differentiating between these samples and other samples on this panel and as a marker for these cancers. Therapeutic targeting of this gene or its protein product with a monoclonal antibody, small molecule, or protein therapeutic will limit or block the extent of tumor cell migration and invasion and tumor metastasis in these tumors.


Panel 3D Summary: Ag271b Expression of this gene was highest in a gastric cancer cell line. Expression of this gene was detected in a number of cancer cell lines, a result that is consistent with the expression in Panel 1. This gene plays a role in cell proliferation. Inhibition of the expression or function of this gene or gene product is a therapeutic avenue for the treatment of cancer or other disease that involve cell proliferation.


Panel 4.1D Summary: Ag271b This gene, which encodes the extracellular domain of an immunoglobin domain containing membrane protein, was expressed in panel 4.1D in the following resting and cytokine-activated cells and tissues: HUVEC, lung microvascular endothelial cells, small airway epithelium, coronary artery smooth muscle cells, astrocytes, lung fibroblasts, and dermal fibroblasts. This gene or its gene product is useful as a target for therapeutic antibodies, small molecules or protein drugs which antagonize the function of the Ig domain-containing protein and reduce or eliminate the symptoms in patients with inflammatory diseases and autoimmune diseases, such as multiple sclerosis, chronic obstructive pulmonary disease, asthma, emphysema, rheumatoid arthritis, or psoriasis.


M. CG51622-01: Von Ebner Minor Salivary Gland Protein


Expression of gene CG51622-01 was assessed using the primer-probe set Ag719, described in Table MA. Results of the RTQ-PCR runs are shown in Tables MB and MC.

TABLE MAProbe Name Ag719StartSEQ IDPrimersSequencesLengthPositionNoForward5′-ccaggctgaaggtcatcac-3′192601309ProbeTET-5′-ctaacatcctccagctgcaggtgaag-3′-262811310TAMRAReverse5′-gactagcagctcctggtcatt-3′213161311









TABLE MB










Panel 1.2












Tissue Name
A
B
C
















Endothelial cells
0.0
0.0
0.0



Heart (Fetal)
0.0
0.0
0.0



Pancreas
0.1
0.0
0.0



Pancreatic ca. CAPAN 2
0.0
0.0
0.0



Adrenal Gland
0.0
0.0
0.0



Thyroid
0.1
0.0
0.0



Salivary gland
1.6
0.8
1.8



Pituitary gland
0.3
0.0
0.0



Brain (fetal)
0.0
0.0
0.0



Brain (whole)
0.0
0.0
0.0



Brain (amygdala)
0.0
0.0
0.0



Brain (cerebellum)
0.0
0.0
0.0



Brain (hippocampus)
0.0
0.0
0.0



Brain (thalamus)
0.0
0.0
0.0



Cerebral Cortex
0.0
0.0
0.0



Spinal cord
0.1
0.0
0.1



glio/astro U87-MG
0.0
0.0
0.0



glio/astro U-118-MG
0.0
0.0
0.0



astrocytoma SW1783
0.0
0.0
0.0



neuro*; met SK-N-AS
0.0
0.0
0.0



astrocytoma SF-539
0.0
0.0
0.0



astrocytoma SNB-75
0.0
0.0
0.0



glioma SNB-19
0.0
0.0
0.0



glioma U251
0.0
0.0
0.0



glioma SF-295
0.0
0.0
0.0



Heart
0.0
0.0
0.0



Skeletal Muscle
0.0
0.0
0.0



Bone marrow
0.0
0.0
0.0



Thymus
0.0
0.0
0.0



Spleen
0.0
0.0
0.0



Lymph node
0.3
0.1
0.2



Colorectal Tissue
0.0
0.0
0.0



Stomach
2.3
3.4
7.3



Small intestine
0.0
0.0
0.0



Colon ca. SW480
0.0
0.0
0.0



Colon ca.* SW620 (SW480 met)
0.0
0.0
0.0



Colon ca. HT29
0.0
0.0
0.0



Colon ca. HCT-116
0.0
0.0
0.0



Colon ca. CaCo-2
0.0
0.0
0.0



Colon ca. Tissue (ODO3866)
0.0
0.0
0.0



Colon ca. HCC-2998
0.0
0.0
0.0



Gastric ca.* (liver met) NCI-N87
0.1
0.0
0.1



Bladder
0.0
0.0
0.0



Trachea
100.0
100.0
100.0



Kidney
0.0
0.0
0.0



Kidney (fetal)
0.0
0.0
0.0



Renal ca. 786-0
0.0
0.0
0.0



Renal ca. A498
0.0
0.0
0.0



Renal ca. RXF 393
0.0
0.0
0.0



Renal ca. ACHN
0.0
0.0
0.0



Renal ca. UO-31
0.0
0.0
0.0



Renal ca. TK-10
0.0
0.0
0.0



Liver
0.0
0.0
0.0



Liver (fetal)
0.0
0.0
0.0



Liver ca. (hepatoblast) HepG2
0.0
0.0
0.0



Lung
5.2
2.3
4.9



Lung (fetal)
1.0
0.3
0.6



Lung ca. (small cell) LX-1
0.0
0.0
0.0



Lung ca. (small cell) NCI-H69
0.0
0.0
0.0



Lung ca. (s. cell var.) SHP-77
0.0
0.0
0.0



Lung ca. (large cell)NCI-H460
0.0
0.0
0.0



Lung ca. (non-sm. cell) A549
0.1
0.0
0.1



Lung ca. (non-s. cell) NCI-H23
0.0
0.0
0.0



Lung ca. (non-s. cell) HOP-62
0.0
0.0
0.0



Lung ca. (non-s. cl) NCI-H522
0.0
0.0
0.0



Lung ca. (squam.) SW 900
0.3
0.1
0.4



Lung ca. (squam.) NCI-H596
0.0
0.0
0.0



Mammary gland
0.1
0.0
0.0



Breast ca.* (pl. ef) MCF-7
0.0
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0
0.0



Breast ca.* (pl. ef) T47D
0.0
0.0
0.0



Breast ca. BT-549
0.0
0.0
0.0



Breast ca. MDA-N
0.0
0.0
0.0



Ovary
0.0
0.0
0.0



Ovarian ca. OVCAR-3
0.0
0.0
0.0



Ovarian ca. OVCAR-4
0.0
0.0
0.0



Ovarian ca. OVCAR-5
0.9
0.3
0.4



Ovarian ca. OVCAR-8
0.0
0.0
0.0



Ovarian ca. IGROV-1
0.0
0.0
0.0



Ovarian ca. (ascites) SK-OV-3
0.0
0.0
0.0



Uterus
0.0
0.0
0.0



Placenta
0.0
0.0
0.0



Prostate
0.0
0.0
0.0



Prostate ca.* (bone met) PC-3
0.0
0.0
0.0



Testis
0.2
0.0
0.1



Melanoma Hs688(A).T
0.0
0.0
0.0



Melanoma* (met) Hs688(B).T
0.0
0.0
0.0



Melanoma UACC-62
0.1
0.0
0.1



Melanoma M14
0.0
0.0
0.0



Melanoma LOX IMVI
0.0
0.0
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0
0.0









Column A - Rel. Exp. (%) Ag719, Run 115164487






Column B - Rel. Exp. (%) Ag719, Run 123273033






Column C - Rel. Exp. (%) Ag719, Run 124111545














TABLE MC










Panel 4D









Tissue Name
A
B












Secondary Th1 act
0.0
0.0


Secondary Th2 act
0.0
0.3


Secondary Tr1 act
0.0
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
0.0


Secondary Tr1 rest
0.0
0.0


Primary Th1 act
0.5
0.0


Primary Th2 act
0.5
0.0


Primary Tr1 act
0.0
0.0


Primary Th1 rest
0.3
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
0.0


CD45RO CD4 lymphocyte act
0.0
0.0


CD8 lymphocyte act
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0


Secondary CD8 lymphocyte act
0.2
0.0


CD4 lymphocyte none
0.0
0.0


2ry Th1/Th2/TM_anti-CD95 CH11
0.0
0.0


LAK cells rest
0.0
0.0


LAK cells IL-2
0.2
0.0


LAK cells IL-2 + IL-12
0.0
0.0


LAK cells IL-2 + IFN gamma
0.3
0.2


LAK cells IL-2 + IL-18
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0


NK Cells IL-2 rest
0.0
0.0


Two Way MLR 3 day
0.0
0.0


Two Way MLR 5 day
0.0
0.0


Two Way MLR 7 day
0.0
0.0


PBMC rest
0.0
0.0


PBMC PWM
0.0
0.0


PBMC PHA-L
0.0
0.0


Ramos (B cell) none
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0


B lymphocytes PWM
0.0
0.0


B lymphocytes CD40L and IL-4
0.0
0.0


EOL-1 dbcAMP
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0


Dendritic cells none
0.0
0.0


Dendritic cells LPS
0.0
0.0


Dendritic cells anti-CD40
0.0
0.0


Monocytes rest
0.0
0.0


Monocytes LPS
0.3
0.0


Macrophages rest
0.0
0.0


Macrophages LPS
0.0
0.0


HUVEC none
0.0
0.0


HUVEC starved
0.0
0.0


HUVEC IL-1beta
0.0
0.0


HUVEC IFN gamma
0.0
0.5


HUVEC TNF alpha + IFN gamma
0.5
0.0


HUVEC TNF alpha + IL4
0.0
0.0


HUVEC IL-11
0.0
0.0


Lung Microvascular EC none
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
0.0
0.0


Microvascular Dermal EC none
0.0
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.3
0.0


Bronchial epithelium TNFalpha + IL1beta
0.0
0.0


Small airway epithelium none
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
0.0
0.0


Coronery artery SMC rest
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
0.0
0.0


Astrocytes TNFalpha + IL-1 beta
0.5
0.0


KU-812 (Basophil) rest
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0


CCD1106 (Keratinocytes) none
0.0
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.3
0.0


Liver cirrhosis
1.6
1.9


Lupus kidney
0.3
0.0


NCI-H292 none
88.9
86.5


NCI-H292 IL-4
64.2
56.6


NCI-H292 IL-9
68.8
55.1


NCI-H292 IL-13
47.0
53.2


NCI-H292 IFN gamma
30.6
19.2


HPAEC none
0.0
0.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast none
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.2
0.0


Lung fibroblast IL-4
0.0
0.0


Lung fibroblast IL-9
0.1
0.0


Lung fibroblast IL-13
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.0


Dermal fibroblast IFN gamma
0.0
0.0


Dermal fibroblast IL-4
0.0
0.0


IBD Colitis 2
0.0
0.0


IBD Crohn's
1.3
0.4


Colon
0.3
1.4


Lung
100.0
100.0


Thymus
1.0
0.0


Kidney
0.8
0.8







Column A - Rel. Exp. (%) Ag719, Run 138167117





Column B - Rel. Exp. (%) Ag719, Run 138273090







Panel 1.2 Summary: Ag719 Highest expression of this gene was detected in the trachea (CTs=18-19). Low to moderate expression was also seen in a few cancer cell lines derived from melanoma, lung, and ovarian cancers. Low expression of this gene is also seen in stomach, pancreas, lung, mammary gland, bladder and spinal cord.


Panel 4D Summary: Ag719 Highest expression of this gene was detected in lung (CTs=28-29). Moderate expression of this gene was also seen in resting and activated NCI-H292.


N. CG51622-03: Von Ebner Minor Salivary Gland Protein


Expression of gene CG51622-03 was assessed using the primer-probe set Ag5814, described in Table NA. Results of the RTQ-PCR runs are shown in Table NB.

TABLE NAProbe Name Ag5814StartSEQ IDPrimersSequencesLengthPositionNoForward5′-ccaggagctgctagtcaaga-3′203581312ProbeTET-5′-ctggattcaacacgctctccttcctg-3′-263961313TAMRAReverse5′-aggaggttcatgacctgctt-3′204371314









TABLE NB










General_screening_panel_v1.5










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
0.2



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.1



Placenta
0.0



Uterus Pool
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
4.2



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.0



Ovary
9.0



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.0



Trachea
100.0



Lung
0.0



Fetal Lung
0.0



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.1



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
0.0



Liver ca. HepG2
0.0



Kidney Pool
0.0



Fetal Kidney
0.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.0



Gastric ca. (liver met.) NCI-N87
0.2



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.2



Colon cancer tissue
0.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.0



Small Intestine Pool
0.2



Stomach Pool
0.0



Bone Marrow Pool
0.0



Fetal Heart
0.0



Heart Pool
0.0



Lymph Node Pool
0.0



Fetal Skeletal Muscle
0.0



Skeletal Muscle Pool
0.0



Spleen Pool
0.0



Thymus Pool
0.0



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.0



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
0.0



Brain (fetal)
0.0



Brain (Hippocampus) Pool
0.0



Cerebral Cortex Pool
0.0



Brain (Substantia nigra) Pool
0.0



Brain (Thalamus) Pool
0.0



Brain (whole)
0.0



Spinal Cord Pool
0.0



Adrenal Gland
0.0



Pituitary gland Pool
0.0



Salivary Gland
0.3



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.2



Pancreas Pool
0.0









Column A - Rel. Exp. (%) Ag5814, Run 244897723







General_screening_panel_v1.5 Summary: Ag5814 High levels of expression of this gene were seen in the trachea (CT=27).


O CG51622-04: Von Ebner Minor Salivary Gland Protein


Expression of gene CG51622-04 was assessed using the primer-probe set Ag5210, described in Table OA. Results of the RTQ-PCR runs are shown in Table OB.

TABLE OAProbe Name Ag5210StartSEQ IDPrimersSequencesLengthPositionNoForward5′-aagaagcatgagacgcagact-3′21871315ProbeTET-5′-caaagaactcccagtgcaggacaatg-3′-261161316TAMRAReverse5′-actgtcctccactcccatgt-3′201651317









TABLE OB










General_screening_panel_v1.5










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
2.0



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.0



Placenta
0.0



Uterus Pool
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
7.3



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.0



Ovary
9.9



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.0



Trachea
100.0



Lung
0.0



Fetal Lung
0.5



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.5



Lung ca. NCI-H526
0.5



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
7.7



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
0.0



Liver ca. HepG2
0.0



Kidney Pool
0.0



Fetal Kidney
0.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.0



Gastric ca. (liver met.) NCI-N87
0.9



Gastric ca. KATO III
0.2



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
0.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.0



Small Intestine Pool
0.2



Stomach Pool
0.2



Bone Marrow Pool
1.3



Fetal Heart
0.0



Heart Pool
0.0



Lymph Node Pool
0.0



Fetal Skeletal Muscle
0.0



Skeletal Muscle Pool
0.0



Spleen Pool
0.0



Thymus Pool
0.2



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.5



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
0.3



Brain (fetal)
0.1



Brain (Hippocampus) Pool
0.2



Cerebral Cortex Pool
0.3



Brain (Substantia nigra) Pool
0.2



Brain (Thalamus) Pool
0.0



Brain (whole)
0.9



Spinal Cord Pool
0.0



Adrenal Gland
0.0



Pituitary gland Pool
0.0



Salivary Gland
0.2



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.8



Pancreas Pool
0.2









Column A - Rel. Exp. (%) Ag5210, Run 228727456







General_screening_panel_v1.5 Summary: Ag5210 Moderate expression was seen in the trachea (CT=30).


P. CG51622-05: Von Ebner Minor Salivary Gland Protein


Expression of gene CG51622-05 was assessed using the primer-probe set Ag5103, described in Table PA. Results of the RTQ-PCR runs are shown in Tables PB, PC, PD and PE.

TABLE PAProbe Name Ag5103StartSEQ IDPrimersSequencesLengthPositionNoForward5′-agtgaaaaaccagctggtgaa-3′216611318ProbeTET-5′-catttccctcagcattgaccgtctg-3′-256881319TAMRAReverse5′-ggcaggatacagaaggtcaaa-3′217161320









TABLE PB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
0.0



110980 COPD-F
0.0



110968 COPD-M
0.0



110977 COPD-M
0.0



110989 Emphysema-F
0.0



110992 Emphysema-F
0.6



110993 Emphysema-F
0.0



110994 Emphysema-F
0.0



110995 Emphysema-F
0.0



110996 Emphysema-F
0.0



110997 Asthma-M
9.2



111001 Asthma-F
0.0



111002 Asthma-F
0.0



111003 Atopic Asthma-F
0.0



111004 Atopic Asthma-F
0.0



111005 Atopic Asthma-F
0.0



111006 Atopic Asthma-F
0.0



111417 Allergy-M
0.0



112347 Allergy-M
0.0



112349 Normal Lung-F
0.0



112357 Normal Lung-F
0.0



112354 Normal Lung-M
0.0



112374 Crohns-F
0.0



112389 Match Control Crohns-F
0.0



112375 Crohns-F
0.0



112732 Match Control Crohns-F
0.0



112725 Crohns-M
0.0



112387 Match Control Crohns-M
0.0



112378 Crohns-M
0.0



112390 Match Control Crohns-M
0.0



112726 Crohns-M
0.0



112731 Match Control Crohns-M
6.3



112380 Ulcer Col-F
0.0



112734 Match Control Ulcer Col-F
54.0



112384 Ulcer Col-F
0.0



112737 Match Control Ulcer Col-F
0.0



112386 Ulcer Col-F
0.0



112738 Match Control Ulcer Col-F
0.0



112381 Ulcer Col-M
0.0



112735 Match Control Ulcer Col-M
0.0



112382 Ulcer Col-M
0.0



112394 Match Control Ulcer Col-M
0.0



112383 Ulcer Col-M
0.0



112736 Match Control Ulcer Col-M
0.0



112423 Psoriasis-F
0.0



112427 Match Control Psoriasis-F
0.0



112418 Psoriasis-M
0.0



112723 Match Control Psoriasis-M
0.0



112419 Psoriasis-M
0.0



112424 Match Control Psoriasis-M
0.0



112420 Psoriasis-M
0.0



112425 Match Control Psoriasis-M
0.0



104689 (MF) OA Bone-Backus
0.0



104690 (MF) Adj “Normal” Bone-Backus
0.0



104691 (MF) OA Synovium-Backus
0.0



104692 (BA) OA Cartilage-Backus
0.0



104694 (BA) OA Bone-Backus
0.0



104695 (BA) Adj “Normal” Bone-Backus
0.0



104696 (BA) OA Synovium-Backus
0.0



104700 (SS) OA Bone-Backus
0.0



104701 (SS) Adj “Normal” Bone-Backus
0.0



104702 (SS) OA Synovium-Backus
0.0



117093 OA Cartilage Rep7
0.0



112672 OA Bone5
0.0



112673 OA Synovium5
0.0



112674 OA Synovial Fluid cells5
0.0



117100 OA Cartilage Rep14
0.0



112756 OA Bone9
0.0



112757 OA Synovium9
0.0



112758 OA Synovial Fluid Cells9
0.0



117125 RA Cartilage Rep2
0.0



113492 Bone2 RA
100.0



113493 Synovium2 RA
35.6



113494 Syn Fluid Cells RA
53.6



113499 Cartilage4 RA
4.0



113500 Bone4 RA
2.7



113501 Synovium4 RA
1.0



113502 Syn Fluid Cells4 RA
0.9



113495 Cartilage3 RA
6.0



113496 Bone3 RA
3.2



113497 Synovium3 RA
4.4



113498 Syn Fluid Cells3 RA
6.3



117106 Normal Cartilage Rep20
0.0



113663 Bone3 Normal
0.0



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
0.0



117107 Normal Cartilage Rep22
0.0



113667 Bone4 Normal
0.0



113668 Synovium4 Normal
0.0



113669 Syn Fluid Cells4 Normal
0.0









Column A - Rel. Exp. (%) Ag5103, Run 229314009














TABLE PC










General_screening_panel_v1.5










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
0.0



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.0



Placenta
0.0



Uterus Pool
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.5



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.0



Ovary
3.6



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.0



Trachea
100.0



Lung
0.0



Fetal Lung
0.2



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.0



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
0.0



Liver ca. HepG2
0.0



Kidney Pool
0.0



Fetal Kidney
0.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.0



Gastric ca. (liver met.) NCI-N87
0.1



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
0.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.0



Small Intestine Pool
0.0



Stomach Pool
0.0



Bone Marrow Pool
0.1



Fetal Heart
0.0



Heart Pool
0.0



Lymph Node Pool
0.0



Fetal Skeletal Muscle
0.0



Skeletal Muscle Pool
0.0



Spleen Pool
0.0



Thymus Pool
0.0



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.0



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
0.0



Brain (fetal)
0.0



Brain (Hippocampus) Pool
0.0



Cerebral Cortex Pool
0.0



Brain (Substantia nigra) Pool
0.0



Brain (Thalamus) Pool
0.0



Brain (whole)
0.0



Spinal Cord Pool
0.0



Adrenal Gland
0.0



Pituitary gland Pool
0.0



Salivary Gland
0.2



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
0.0









Column A - Rel. Exp. (%) Ag5103, Run 228753956














TABLE PD










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.9



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
0.0



NCI-H292 none
14.7



NCI-H292 IL-4
23.0



NCI-H292 IL-9
33.2



NCI-H292 IL-13
13.2



NCI-H292 IFN gamma
9.6



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
100.0



Dermal fibroblast IL-4
0.0



Dermal Fibroblasts rest
0.0



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.0



Colon
2.0



Lung
24.7



Thymus
0.3



Kidney
0.0









Column A - Rel. Exp. (%) Ag5103, Run 226160624














TABLE PE










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
0.0



Colon cancer NAT 1
0.0



Colon cancer 2
0.0



Colon cancer NAT 2
0.0



Colon cancer 3
0.0



Colon cancer NAT 3
0.0



Colon malignant cancer 4
0.0



Colon normal adjacent tissue 4
0.0



Lung cancer 1
6.6



Lung NAT 1
19.8



Lung cancer 2
0.8



Lung NAT 2
0.0



Squamous cell carcinoma 3
16.0



Lung NAT 3
59.0



metastatic melanoma 1
0.0



Melanoma 2
0.0



Melanoma 3
0.0



metastatic melanoma 4
8.0



metastatic melanoma 5
100.0



Bladder cancer 1
0.0



Bladder cancer NAT 1
0.0



Bladder cancer 2
0.0



Bladder cancer NAT 2
0.0



Bladder cancer NAT 3
0.2



Bladder cancer NAT 4
0.0



Prostate adenocarcinoma 1
0.0



Prostate adenocarcinoma 2
0.0



Prostate adenocarcinoma 3
0.0



Prostate adenocarcinoma 4
0.4



Prostate cancer NAT 5
0.0



Prostate adenocarcinoma 6
0.0



Prostate adenocarcinoma 7
0.0



Prostate adenocarcinoma 8
0.5



Prostate adenocarcinoma 9
2.0



Prostate cancer NAT 10
0.0



Kidney cancer 1
0.0



KidneyNAT 1
0.0



Kidney cancer 2
0.0



Kidney NAT 2
0.0



Kidney cancer 3
0.0



Kidney NAT 3
0.0



Kidney cancer 4
0.0



Kidney NAT 4
0.0









Column A- Rel. Exp. (%) Ag5103, Run259939204







AI_comprehensive panel_v1.0 Summary: Ag5103 Moderate levels of expression were seen in three RA samples, with highest expression in a bone sample from an RA patient (CT=30). Therapeutics targeting of this gene or its protein product is useful for the reduction and inhibition of the inflammation in RA.


General_screening_panel_v1.5 Summary: Ag5103 Extremely high levels of expression were seen in the trachea (CT=23).


Panel 4.1D Summary: AG5103 Highest expression of this gene was detected in IFN gamma treated dermal fibroblasts (CT=31.6). Prominent expression was also seen in a cluster of treated and untreated NCI-H292 cells, a mucoepidermoid cell line. In comparison, expression in the normal lung was relatively low. The expression of the transcript in a cell line that is often used as a model for airway epithelium (NCI-H292 cells) demonstrates that this transcript is important in the proliferation or activation of airway epithelium. Therapeutics designed with the protein encoded for by the transcript are important in the treatment of diseases which include lung airway inflammation such as asthma and COPD.


general oncology screening panel_v—2.4 Summary: Ag5103 Highest expression was seen in a melanoma sample (CT=31). Prominent expression was also seen in two samples derived from normal lung tissue.


Q. CG51821-01: Sialoadhesin


Expression of gene CG51821-01 was assessed using the primer-probe set Ag274, described in Table QA. Results of the RTQ-PCR runs are shown in Tables QB, QC, QD, QE, QF and QG.

TABLE QAProbe Name Ag274PrimersSequencesLengthStart PositionSEQ ID NoForward5′-cagaacagatgtattccccttggt-3′2448351321ProbeTET-5′-ctcagcgcctcgatgtccaccc-3′-TAMRA2248051322Reverse5′-ctggcttcccccaatgc-3′1747831323









TABLE QB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
4.3



110980 COPD-F
1.6



110968 COPD-M
5.2



110977 COPD-M
2.7



110989 Emphysema-F
2.2



110992 Emphysema-F
0.5



110993 Emphysema-F
4.0



110994 Emphysema-F
4.3



110995 Emphysema-F
5.7



110996 Emphysema-F
2.0



110997 Asthma-M
1.6



111001 Asthma-F
2.2



111002 Asthma-F
4.4



111003 Atopic Asthma-F
4.0



111004 Atopic Asthma-F
3.6



111005 Atopic Asthma-F
2.4



111006 Atopic Asthma-F
0.5



111417 Allergy-M
2.1



112347 Allergy-M
0.1



112349 Normal Lung-F
0.0



112357 Normal Lung-F
8.4



112354 Normal Lung-M
2.6



112374 Crohns-F
4.4



112389 Match Control Crohns-F
2.2



112375 Crohns-F
3.9



112732 Match Control Crohns-F
3.4



112725 Crohns-M
0.6



112387 Match Control Crohns-M
4.3



112378 Crohns-M
0.2



112390 Match Control Crohns-M
2.3



112726 Crohns-M
3.5



112731 Match Control Crohns-M
3.1



112380 Ulcer Col-F
1.1



112734 Match Control Ulcer Col-F
8.8



112384 Ulcer Col-F
4.9



112737 Match Control Ulcer Col-F
0.4



112386 Ulcer Col-F
8.4



112738 Match Control Ulcer Col-F
6.3



112381 Ulcer Col-M
0.5



112735 Match Control Ulcer Col-M
0.9



112382 Ulcer Col-M
3.3



112394 Match Control Ulcer Col-M
2.8



112383 Ulcer Col-M
5.3



112736 Match Control Ulcer Col-M
5.5



112423 Psoriasis-F
2.2



112427 Match Control Psoriasis-F
1.8



112418 Psoriasis-M
7.8



112723 Match Control Psoriasis-M
3.3



112419 Psoriasis-M
4.4



112424 Match Control Psoriasis-M
1.1



112420 Psoriasis-M
6.2



112425 Match Control Psoriasis-M
1.5



104689 (MF) OA Bone-Backus
28.5



104690 (MF) Adj “Normal” Bone-Backus
19.6



104691 (MF) OA Synovium-Backus
100.0



104692 (BA) OA Cartilage-Backus
0.7



104694 (BA) OA Bone-Backus
42.3



104695 (BA) Adj “Normal” Bone-Backus
27.4



104696 (BA) OA Synovium-Backus
71.2



104700 (SS) OA Bone-Backus
16.4



104701 (SS) Adj “Normal” Bone-Backus
29.7



104702 (SS) OA Synovium-Backus
67.8



117093 OA Cartilage Rep7
1.8



112672 OA Bone5
8.8



112673 OA Synovium5
3.5



112674 OA Synovial Fluid cells5
3.7



117100 OA Cartilage Rep14
1.8



112756 OA Bone9
2.5



112757 OA Synovium9
4.7



112758 OA Synovial Fluid Cells9
9.0



117125 RA Cartilage Rep2
4.6



113492 Bone2 RA
6.6



113493 Synovium2 RA
4.4



113494 Syn Fluid Cells RA
5.8



113499 Cartilage4 RA
8.3



113500 Bone4 RA
7.9



113501 Synovium4 RA
6.9



113502 Syn Fluid Cells4 RA
4.1



113495 Cartilage3 RA
5.6



113496 Bone3 RA
5.8



113497 Synovium3 RA
4.3



113498 Syn Fluid Cells3 RA
9.2



117106 Normal Cartilage Rep20
2.0



113663 Bone3 Normal
0.0



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
0.1



117107 Normal Cartilage Rep22
1.4



113667 Bone4 Normal
2.5



113668 Synovium4 Normal
1.4



113669 Syn Fluid Cells4 Normal
3.1









Column A - Rel. Exp. (%) Ag274, Run 228157466














TABLE QC










General_screenmg_panel_v1.4











Tissue Name
A
B















Adipose
20.4
23.5



Melanoma* Hs688(A).T
0.0
0.0



Melanoma* Hs688(B).T
0.6
1.5



Melanoma* M14
0.0
0.0



Melanoma* LOXIMVI
3.1
0.0



Melanoma* SK-MEL-5
0.0
1.9



Squamous cell carcinoma SCC-4
1.0
0.0



Testis Pool
5.7
7.1



Prostate ca.* (bone met) PC-3
0.0
1.1



Prostate Pool
1.6
2.0



Placenta
45.4
51.4



Uterus Pool
2.2
1.2



Ovarian ca. OVCAR-3
0.0
0.0



Ovarian ca. SK-OV-3
0.0
0.8



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
0.0
2.6



Ovarian ca. IGROV-1
0.5
1.0



Ovarian ca. OVCAR-8
0.8
2.9



Ovary
12.8
9.9



Breast ca. MCF-7
0.7
0.0



Breast ca. MDA-MB-231
0.4
2.4



Breast ca. BT 549
0.0
0.0



Breast ca. T47D
1.0
1.6



Breast ca. MDA-N
0.6
1.3



Breast Pool
8.7
6.1



Trachea
14.3
21.3



Lung
3.8
5.3



Fetal Lung
6.7
4.6



Lung ca. NCI-N417
0.0
0.0



Lung ca. LX-1
0.3
0.6



Lung ca. NCI-H146
1.0
0.0



Lung ca. SHP-77
0.8
1.0



Lung ca. A549
1.1
0.0



Lung ca. NCI-H526
0.0
0.0



Lung ca. NCI-H23
0.0
0.0



Lung ca. NCI-H460
0.0
1.1



Lung ca. HOP-62
0.0
5.1



Lung ca. NCI-H522
0.0
0.0



Liver
11.7
13.5



Fetal Liver
26.6
26.2



Liver ca. HepG2
0.0
0.7



Kidney Pool
7.0
13.0



Fetal Kidney
3.1
3.2



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.0
0.0



Renal ca. ACHN
0.0
1.8



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.0
0.6



Bladder
100.0
100.0



Gastric ca. (liver met.) NCI-N87
2.0
2.1



Gastric ca. KATO III
0.0
2.0



Colon ca. SW-948
0.0
0.0



Colon ca. SW480
1.2
0.3



Colon ca.* (SW480 met) SW620
0.5
0.5



Colon ca. HT29
1.3
0.0



Colon ca. HCT-116
0.0
0.8



Colon ca. CaCo-2
1.0
0.0



Colon cancer tissue
26.4
32.3



Colon ca. SW1116
1.6
0.8



Colon ca. Colo-205
0.0
0.0



Colon ca. SW-48
0.0
1.3



Colon Pool
12.1
9.7



Small Intestine Pool
5.3
3.8



Stomach Pool
8.1
9.8



Bone Marrow Pool
4.8
7.9



Fetal Heart
0.4
1.2



Heart Pool
5.3
8.2



Lymph Node Pool
8.8
11.7



Fetal Skeletal Muscle
5.0
5.5



Skeletal Muscle Pool
12.2
13.0



Spleen Pool
29.7
28.1



Thymus Pool
12.6
10.9



CMS cancer (glio/astro) U87-MG
0.9
1.2



CMS cancer (glio/astro) U-118-MG
1.7
0.8



CMS cancer (neuro; met) SK-N-AS
0.5
0.0



CMS cancer (astro) SF-539
0.0
0.5



CMS cancer (astro) SNB-75
1.3
1.1



CMS cancer (glio) SNB-19
0.2
0.0



CNS cancer (glio) SF-295
0.1
0.5



Brain (Amygdala) Pool
2.6
2.5



Brain (cerebellum)
0.8
1.5



Brain (fetal)
2.3
3.3



Brain (Hippocampus) Pool
1.0
2.3



Cerebral Cortex Pool
1.2
0.6



Brain (Substantia nigra) Pool
1.3
0.0



Brain (Thalamus) Pool
1.5
1.5



Brain (whole)
5.7
4.7



Spinal Cord Pool
1.1
2.2



Adrenal Gland
19.1
29.1



Pituitary gland Pool
0.5
4.1



Salivary Gland
3.8
4.5



Thyroid (female)
6.3
5.4



Pancreatic ca. CAPAN2
0.0
0.0



Pancreas Pool
9.3
9.3









Column A - Rel. Exp. (%) Ag274, Run 216607761






Column B - Rel. Exp. (%) Ag274, Run 220361695














TABLE QD










Panel 2D










Tissue Name
A














Normal Colon
23.3



CC Well to Mod Diff (ODO3866)
13.2



CC Margin (ODO3866)
10.0



CC Gr.2 rectosigmoid (ODO3868)
6.0



CC Margin (ODO3868)
6.0



CC Mod Diff (ODO3920)
3.1



CC Margin (ODO3920)
10.9



CC Gr.2 ascend colon (ODO3921)
10.2



CC Margin (ODO3921)
25.5



CC from Partial Hepatectomy (ODO4309) Mets
9.7



Liver Margin (ODO4309)
48.3



Colon mets to lung (OD04451-01)
14.4



Lung Margin (OD04451-02)
17.8



Normal Prostate 6546-1
11.0



Prostate Cancer (OD04410)
9.7



Prostate Margin (OD04410)
4.1



Prostate Cancer (OD04720-01)
2.0



Prostate Margin (OD04720-02)
4.5



Normal Lung 061010
66.0



Lung Met to Muscle (ODO4286)
100.0



Muscle Margin (ODO4286)
72.2



Lung Malignant Cancer (OD03126)
35.4



Lung Margin (OD03126)
68.3



Lung Cancer (OD04404)
29.5



Lung Margin (OD04404)
35.6



Lung Cancer (OD04565)
12.4



Lung Margin (OD04565)
36.1



Lung Cancer (OD04237-01)
18.6



Lung Margin (OD04237-02)
22.4



Ocular Mel Met to Liver (ODO4310)
4.2



Liver Margin (ODO4310)
24.3



Melanoma Mets to Lung (OD04321)
5.8



Lung Margin (OD04321)
29.7



Normal Kidney
12.2



Kidney Ca, Nuclear grade 2 (OD04338)
16.5



Kidney Margin (OD04338)
6.2



Kidney Ca Nuclear grade 1/2 (OD04339)
23.2



Kidney Margin (OD04339)
4.6



Kidney Ca, Clear cell type (OD04340)
11.8



Kidney Margin (OD04340)
5.8



Kidney Ca, Nuclear grade 3 (OD04348)
56.6



Kidney Margin (OD04348)
22.8



Kidney Cancer (OD04622-01)
24.0



Kidney Margin (OD04622-03)
2.6



Kidney Cancer (OD04450-01)
0.9



Kidney Margin (OD04450-03)
2.6



Kidney Cancer 8120607
5.7



Kidney Margin 8120608
3.6



Kidney Cancer 8120613
5.7



Kidney Margin 8120614
3.5



Kidney Cancer 9010320
61.1



Kidney Margin 9010321
24.1



Normal Uterus
5.4



Uterus Cancer 064011
5.1



Normal Thyroid
5.9



Thyroid Cancer 064010
3.1



Thyroid Cancer A302152
3.3



Thyroid Margin A302153
13.5



Normal Breast
13.2



Breast Cancer (OD04566)
40.9



Breast Cancer (OD04590-01)
29.5



Breast Cancer Mets (OD04590-03)
26.1



Breast Cancer Metastasis (OD04655-05)
31.9



Breast Cancer 064006
16.0



Breast Cancer 1024
11.3



Breast Cancer 9100266
3.7



Breast Margin 9100265
4.7



Breast Cancer A209073
5.7



Breast Margin A209073
1.5



Normal Liver
8.4



Liver Cancer 064003
8.4



Liver Cancer 1025
25.3



Liver Cancer 1026
6.4



Liver Cancer 6004-T
40.1



Liver Tissue 6004-N
17.8



Liver Cancer 6005-T
10.2



Liver Tissue 6005-N
18.6



Normal Bladder
60.7



Bladder Cancer 1023
5.1



Bladder Cancer A302173
18.6



Bladder Cancer (OD04718-01)
28.5



Bladder Normal Adjacent (OD04718-03)
48.6



Normal Ovary
0.0



Ovarian Cancer 064008
16.2



Ovarian Cancer (OD04768-07)
16.4



Ovary Margin (OD04768-08)
19.2



Normal Stomach
6.4



Gastric Cancer 9060358
2.0



Stomach Margin 9060359
5.1



Gastric Cancer 9060395
8.4



Stomach Margin 9060394
10.2



Gastric Cancer 9060397
17.4



Stomach Margin 9060396
5.3



Gastric Cancer 064005
14.7









Column A - Rel. Exp. (%) Ag274, Run 170849611














TABLE QE










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
14.7



TE671- Medulloblastoma
51.1



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
0.0



SNB-78- Glioma
10.5



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
10.2



SK-N-SH- Neuroblastoma (metastasis)
10.4



SF-295- Glioblastoma
0.0



Cerebellum
22.7



Cerebellum
9.0



NCI-H292- Mucoepidermoid lung carcinoma
0.0



DMS-114- Small cell lung cancer
6.4



DMS-79- Small cell lung cancer
32.8



NCI-H146- Small cell lung cancer
0.0



NCI-H526- Small cell lung cancer
11.3



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
5.0



NCI-H157- Squamous cell lung cancer (metastasis)
0.0



NCI-H1155- Large cell lung cancer
25.2



NCI-H1299- Large cell lung cancer
0.0



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
11.5



LX-1- Small cell lung cancer
49.7



Colo-205- Colon cancer
0.0



KM12- Colon cancer
22.7



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
9.7



SW1116- Colon adenocarcinoma
6.1



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
7.5



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
0.0



KATO III- Gastric carcinoma
66.0



NCI-SNU-16- Gastric carcinoma
0.0



NCI-SNU-1- Gastric carcinoma
6.3



RF-1- Gastric adenocarcinoma
5.5



RF-48- Gastric adenocarcinoma
10.4



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
11.1



OVCAR-5- Ovarian carcinoma
8.8



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
3.4



Ca Ski- Cervical epidermoid carcinoma (metastasis)
4.2



ES-2- Ovarian clear cell carcinoma
9.7



Ramos- Stimulated with PMA/ionomycin 6 h
5.6



Ramos- Stimulated with PMA/ionomycin 14 h
100.0



MEG-01- Chronic myelogenous leukemia
11.3



(megokaryoblast)



Raji- Burkitt's lymphoma
5.4



Daudi- Burkitt's lymphoma
5.1



U266- B-cell plasmacytoma
7.4



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
0.0



HUT 78- T-cell lymphoma
14.9



U937- Histiocytic lymphoma
9.1



KU-812- Myelogenous leukemia
39.5



769-P- Clear cell renal carcinoma
6.0



Caki-2- Clear cell renal carcinoma
9.7



SW 839- Clear cell renal carcinoma
16.4



Rhabdoid kidney tumor
6.7



Hs766T- Pancreatic carcinoma (LN metastasis)
0.0



CAPAN-1- Pancreatic adenocarcinoma (liver metastasis)
11.3



SU86.86- Pancreatic carcinoma (liver metastasis)
0.0



BxPC-3- Pancreatic adenocarcinoma
0.0



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
4.5



CFPAC-1- Pancreatic ductal adenocarcinoma
7.4



PANC-1 - Pancreatic epithelioid ductal carcinoma
58.6



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
11.1



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
10.8



A204- Rhabdomyosarcoma
0.0



HT-1080- Fibrosarcoma
0.0



MG-63- Osteosarcoma
9.9



SK-LMS-1- Leiomyosarcoma (vulva)
0.0



SJRH30- Rhabdomyosarcoma (met to bone marrow)
3.8



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
0.0



DU 145- Prostate carcinoma (brain metastasis)
5.5



MDA-MB-468- Breast adenocarcinoma
10.8



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp. (%) Ag274, Run 170745273














TABLE QF










Panel 4D









Tissue Name
A
B












Secondary Th1 act
0.0
0.0


Secondary Th2 act
0.4
0.1


Secondary Tr1 act
0.2
0.2


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.9
0.0


Secondary Tr1 rest
0.3
0.0


Primary Th1 act
0.4
0.0


Primary Th2 act
0.3
0.3


Primary Tr1 act
0.0
0.0


Primary Th1 rest
0.4
0.5


Primary Th2 rest
0.6
0.1


Primary Tr1 rest
0.0
0.3


CD45RA CD4 lymphocyte act
0.3
0.2


CD45RO CD4 lymphocyte act
0.0
0.0


CD8 lymphocyte act
0.0
0.0


Secondary CD8 lymphocyte rest
0.7
0.0


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
0.0
0.8


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.3
0.0


LAK cells rest
13.3
12.6


LAK cells IL-2
0.5
0.4


LAK cells IL-2 + IL-12
0.5
1.6


LAK cells IL-2 + IFN gamma
0.8
0.4


LAK cells IL-2 + IL-18
0.5
0.4


LAK cells PMA/ionomycin
5.4
6.9


NK Cells IL-2 rest
0.5
0.1


Two Way MLR 3 day
44.4
38.7


Two Way MLR 5 day
8.0
6.9


Two Way MLR 7 day
0.4
0.5


PBMC rest
1.4
1.7


PBMC PWM
0.7
0.5


PBMC PHA-L
2.6
3.2


Ramos (B cell) none
0.0
0.3


Ramos (B cell) ionomycin
0.0
0.7


B lymphocytes PWM
0.0
0.0


B lymphocytes CD40L and IL-4
0.3
0.6


EOL-1 dbcAMP
0.2
0.4


EOL-1 dbcAMP PMA/ionomycin
0.0
0.3


Dendritic cells none
7.9
6.4


Dendritic cells LPS
32.1
31.6


Dendritic cells anti-CD40
3.8
3.6


Monocytes rest
7.9
8.4


Monocytes LPS
100.0
100.0


Macrophages rest
3.6
3.5


Macrophages LPS
95.3
88.3


HUVEC none
0.2
0.0


HUVEC starved
0.4
0.3


HUVEC IL-1beta
0.5
0.0


HUVEC IFN gamma
0.0
0.1


HUVEC TNF alpha + IFN gamma
0.3
0.1


HUVEC TNF alpha + IL4
0.0
0.2


HUVEC IL-11
0.2
0.0


Lung Microvascular EC none
0.0
0.3


Lung Microvascular EC TNFalpha + IL-1beta
0.0
0.3


Microvascular Dermal EC none
0.0
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.2


Bronchial epithelium TNFalpha + IL1beta
0.0
0.3


Small airway epithelium none
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
0.0
0.1


Coronery artery SMC rest
0.2
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
0.0
0.0


Astrocytes TNFalpha + IL-1beta
1.0
0.3


KU-812 (Basophil) rest
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.4
0.1


CCD1106 (Keratinocytes) none
0.4
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.3
0.9


Liver cirrhosis
2.3
1.5


Lupus kidney
1.8
0.8


NCI-H292 none
0.0
0.4


NCI-H292 IL-4
0.3
0.2


NCI-H292 IL-9
0.0
0.0


NCI-H292 IL-13
0.0
0.2


NCI-H292 IFN gamma
0.0
0.0


HPAEC none
0.3
0.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast none
0.3
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.3
0.0


Lung fibroblast IL-4
0.4
0.4


Lung fibroblast IL-9
0.0
0.0


Lung fibroblast IL-13
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.3


Dermal fibroblast CCD1070 rest
0.7
0.2


Dermal fibroblast CCD1070 TNF alpha
0.4
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.1


Dermal fibroblast IFN gamma
0.0
0.0


Dermal fibroblast IL-4
0.4
0.2


IBD Colitis 2
0.0
0.2


IBD Crohn's
0.6
0.7


Colon
4.1
4.1


Lung
7.5
8.0


Thymus
0.9
0.1


Kidney
4.8
3.0







Column A - Rel. Exp. (%) Ag274, Run 164533655





Column B - Rel. Exp. (%) Ag274, Run 164534874














TABLE QG










Panel 5D










Tissue Name
A














97457_Patient-02go_adipose
100.0



97476_Patient-07sk_skeletal muscle
18.9



97477_Patient-07ut_uterus
15.2



97478_Patient-07pl_placenta
28.9



97481_Patient-08sk_skeletal muscle
17.1



97482_Patient-08ut_uterus
7.4



97483_Patient-08pl_placenta
25.9



97486_Patient-09sk_skeletal muscle
22.1



97487_Patient-09ut_uterus
13.7



97488_Patient-09pl_placenta
6.7



97492_Patient-10ut_uterus
23.0



97493_Patient-10pl_placenta
16.5



97495_Patient-11go_adipose
26.1



97496_Patient-11sk_skeletal muscle
11.1



97497_Patient-11ut_uterus
9.4



97498_Patient-11pl_placenta
14.7



97500_Patient-12go_adipose
25.0



97501_Patient-12sk_skeletal muscle
7.0



97502_Patient-12ut_uterus
37.9



97503_Patient-12pl_placenta
9.5



94721_Donor 2 U - A_Mesenchymal Stem Cells
6.5



94722_Donor 2 U - B_Mesenchymal Stem Cells
0.0



94723_Donor 2 U - C_Mesenchymal Stem Cells
0.0



94709_Donor 2 AM - A_adipose
0.0



94710_Donor 2 AM - B_adipose
10.3



94711_Donor 2 AM - C_adipose
3.6



94712_Donor 2 AD - A_adipose
0.0



94713_Donor 2 AD - B_adipose
3.4



94714_Donor 2 AD - C_adipose
0.0



94742_Donor 3 U - A_Mesenchymal Stem Cells
6.3



94743_Donor 3 U - B_Mesenchymal Stem Cells
7.4



94730_Donor 3 AM - A_adipose
3.0



94731_Donor 3 AM - B_adipose
10.2



94732_Donor 3 AM - C_adipose
6.9



94733_Donor 3 AD - A_adipose
1.6



94734_Donor 3 AD - B_adipose
9.0



94735_Donor 3 AD - C_adipose
6.6



77138_Liver_HepG2untreated
7.4



73556_Heart_Cardiac stromal cells (primary)
0.0



81735_Small Intestine
22.1



72409_Kidney_Proximal Convoluted Tubule
3.2



82685_Small intestine_Duodenum
11.4



90650_Adrenal_Adrenocortical adenoma
15.4



72410_Kidney_HRCE
10.2



72411_Kidney_HRE
2.9



73139_Uterus_Uterine smooth muscle cells
3.2









Column A - Rel. Exp. (%) Ag274, Run 255179442







AI_comprehensive panel_v1.0 Summary: Ag274 Highest expression of this gene was detected in synovium of an osteooarthritis (OA) patient (CT=28.6). This gene showed a higher level of expression in the bone and synovium samples from OA patients relative to samples from rheumatoid arthritis patients and normal tissues. This gene encodes sialoadhesin (CD169), a lectin that binds to alpha-2,6-linked, sialic acid residues. It has been previously reported that expression of sialoadhesin is highly induced in synovial macrophages from patients with rheumatoid arthritis and in animal models of arthritis (Hartnell, A. Blood 97: 288-296; Dijkstra, C. D. Scand. J. Immunol. 26:5132). The expression profile in this panel confirmed expression of this gene in rheumatoid tissue and also demonstrated an upregulation in synovial tissue from OA patients. Modulation of this gene, expressed protein and/or use of antibodies or small molecule drug targeting the gene or gene product is useful in the treatment of osteoarthritis.


Low expression of this gene was also seen in samples from COPD lung, emphysema, atopic asthma, asthma, Crohn's disease (normal matched control and diseased), ulcerative colitis(normal matched control and diseased), and psoriasis (normal matched control and diseased). Therapeutic modulation of this gene or gene product is useful for the amelioration of symptoms/conditions associated with autoimmune and inflammatory disorders including psoriasis, asthma, inflammatory bowel disease, and rheumatoid arthritis.


General_screening13 panel_v1.4 Summary: Ag274 Highest expression of this gene was detected in bladder (CTs=27-29). Expression of this gene was mainly associated with normal tissues in this panel. Among tissues with metabolic or endocrine function, this gene was expressed at moderate levels in pancreas, adipose, adrenal gland, thyroid, pituitary gland, skeletal muscle, heart, liver and the gastrointestinal tract. Therapeutic modulation of this gene or expressed protein is useful in the treatment of endocrine/metabolically related diseases, such as obesity and diabetes.


This gene was expressed at low levels in all regions of the central nervous system examined, including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. Therapeutic modulation of this gene product is useful in the treatment of central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Significant expression of this gene was also seen in a colon cancer cell line. Modulation of this gene or encoded protein is useful in the treatment of colon cancer.


Panel 2D Summary: Ag274 Highest expression of this gene was detected in muscle cancer metastasized from lung (CT=29). Moderate expression of this gene was also seen in normal and cancer samples from colon, lung, kidney, liver, prostate, bladder, ovary and stomach. Therapeutic modulation of this gene or expressed protein is useful in the treatment of these cancers.


Panel 3D Summary: Ag274 Expression of this gene was highest in the Ramos cell line stimulated with PMA and ionomycin for 16 hrs, followed by lower expression in cell lines derived from gastric carcinoma and pancreatic ductal carcinoma, and lower still in small cell lung carcinoma, medulloblastoma and myelogenous leukemia.


Panel 4D Summary: Ag274 Highest expression of this gene was detected in LPS treated monocytes (CTs=27). The expression of this gene was limited to LPS activated monocytes, macrophages and related cell types or derived from monocytes (dendritic cells). The putative sialoadhesin encoded by this gene directs monocyte extravasation into tissues, and acts as a cell:cell interaction molecule. Modulation of this gene, expressed protein and/or use of antibodies is useful for blocking monocyte extravasation and reducing or inhibiting inflammation associated with asthma, psoriasis, emphysema, arthritis, and other inflammatory diseases.


Panel 5D Summary: Ag274 Highest expression of this gene was detected in adipose of a diabetic patient (CT=31). Significant expression of this gene was also seen in skeletal muscle and placenta from obese and/or diabetic patients. Therapeutic modulation of this gene or expressed protein is useful in the treatment of metabolic/endocrine diseases such as obesity and diabetes.


R. CG51992-05: CTCL Tumor Antigens E57-1


Expression of gene CG51992-05 was assessed using the primer-probe sets Ag1920, Ag2775, Ag2776, and Ag5232, described in Tables RA, RB, RC, and RD. Results of the RTQ-PCR runs are shown in Tables RE, RF, RG, and RH.

TABLE RAProbe Name Ag1920PrimersSequencesLengthStart PositionSEQ ID NoForward5′-gctcctggagaatggaaatc-3′209021324ProbeTET-5′-tccctctgaagatcctggcaactttt-3′-TAMRA268761325Reverse5′-caagctactgcaactcaaatcc-3′228391326









TABLE RB










Probe Name Ag2775











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-caggatcttcagagggagattt-3′
22
885
1327






Probe
TET-5′-ctccaggagcagatctctcatctgca-3′-TAMRA
26
912
1328





Reverse
5′-atgttgggagtgaatcacaaac-3′
22
938
1329
















TABLE RC










Probe Name Ag2776











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-aaatgtcatcttcttgcaaagc-3′
22
1333
1330






Probe
TET-5′-tccatttaaataatggcattttgcca-3′-TAMRA
26
1289
1331





Reverse
5′-cagcccaagtttcatgttaaaa-3′
22
1267
1332
















TABLE RD










Probe Name Ag5232











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-ctagatgcaaagctgcaagc-3′
20
531
1333






Probe
TET-5′-aagcaggagcagcagctctgagaaac-3′-TAMRA
26
559
1334





Reverse
5′-cttcttcagattgcgtttgg-3′
20
611
1335
















TABLE RE










General_screening_panel_v1.5










Tissue Name
A














Adipose
3.7



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
1.6



Melanoma* M14
0.2



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
3.1



Testis Pool
7.9



Prostate ca.* (bone met) PC-3
3.9



Prostate Pool
4.1



Placenta
1.4



Uterus Pool
1.3



Ovarian ca. OVCAR-3
1.5



Ovarian ca. SK-OV-3
28.9



Ovarian ca. OVCAR-4
0.3



Ovarian ca. OVCAR-5
2.9



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.1



Ovary
9.4



Breast ca. MCF-7
0.1



Breast ca. MDA-MB-231
11.4



Breast ca. BT 549
71.7



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
4.0



Trachea
3.2



Lung
5.1



Fetal Lung
100.0



Lung ca. NCI-N417
0.0



Lung ca. LX-1
2.2



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
11.3



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
1.5



Lung ca. NCI-H460
94.0



Lung ca. HOP-62
1.2



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
20.9



Liver ca. HepG2
19.8



Kidney Pool
6.0



Fetal Kidney
12.9



Renal ca. 786-0
3.0



Renal ca. A498
1.6



Renal ca. ACHN
1.0



Renal ca. UO-31
8.4



Renal ca. TK-10
7.6



Bladder
38.4



Gastric ca. (liver met.) NCI-N87
4.4



Gastric ca. KATO III
53.2



Colon ca. SW-948
17.9



Colon ca. SW480
1.2



Colon ca.* (SW480 met) SW620
0.1



Colon ca. HT29
1.3



Colon ca. HCT-116
7.3



Colon ca. CaCo-2
7.5



Colon cancer tissue
7.0



Colon ca. SW1116
0.8



Colon ca. Colo-205
0.6



Colon ca. SW-48
1.0



Colon Pool
1.8



Small Intestine Pool
2.5



Stomach Pool
4.1



Bone Marrow Pool
3.4



Fetal Heart
1.7



Heart Pool
7.2



Lymph Node Pool
6.2



Fetal Skeletal Muscle
0.0



Skeletal Muscle Pool
11.8



Spleen Pool
15.0



Thymus Pool
2.2



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.0



CNS cancer (neuro; met) SK-N-AS
0.1



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.3



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala) Pool
1.8



Brain (cerebellum)
1.0



Brain (fetal)
0.4



Brain (Hippocampus) Pool
0.3



Cerebral Cortex Pool
3.1



Brain (Substantia nigra) Pool
0.8



Brain (Thalamus) Pool
2.3



Brain (whole)
0.1



Spinal Cord Pool
2.1



Adrenal Gland
1.5



Pituitary gland Pool
3.2



Salivary Gland
0.4



Thyroid (female)
0.2



Pancreatic ca. CAPAN2
1.6



Pancreas Pool
9.9









Column A - Rel. Exp. (%) Ag5232, Run 229385253














TABLE RF










Panel 1.3D











Tissue Name
A
B















Liver adenocarcinoma
1.9
3.8



Pancreas
1.2
0.8



Pancreatic ca. CAPAN 2
1.4
0.9



Adrenal gland
4.0
10.7



Thyroid
3.3
2.6



Salivary gland
2.3
2.2



Pituitary gland
14.7
11.8



Brain (fetal)
0.2
0.0



Brain (whole)
10.4
9.7



Brain (amygdala)
5.5
3.7



Brain (cerebellum)
0.2
0.7



Brain (hippocampus)
19.5
30.1



Brain (substantia nigra)
2.0
1.8



Brain (thalamus)
8.8
13.4



Cerebral Cortex
20.0
14.3



Spinal cord
3.5
3.7



glio/astro U87-MG
0.5
0.0



glio/astro U-118-MG
0.0
0.0



astrocytoma SW1783
0.2
0.0



neuro*; met SK-N-AS
0.6
0.0



astrocytoma SF-539
0.0
0.0



astrocytoma SNB-75
10.3
4.8



glioma SNB-19
0.0
0.0



glioma U251
0.3
0.0



glioma SF-295
0.2
0.0



Heart (fetal)
1.7
1.0



Heart
8.9
11.2



Skeletal muscle (fetal)
2.7
0.7



Skeletal muscle
1.7
2.0



Bone marrow
2.7
3.9



Thymus
0.0
0.0



Spleen
16.3
9.2



Lymph node
4.3
4.1



Colorectal
42.0
12.5



Stomach
29.9
7.1



Small intestine
38.4
40.6



Colon ca. SW480
1.2
0.0



Colon ca.* SW620(SW480 met)
0.0
0.0



Colon ca. HT29
4.7
0.5



Colon ca. HCT-116
5.3
3.5



Colon ca. CaCo-2
10.7
4.7



Colon ca. tissue(ODO3866)
6.3
3.5



Colon ca. HCC-2998
5.6
4.7



Gastric ca.* (liver met) NCI-N87
7.7
3.3



Bladder
22.1
21.5



Trachea
15.3
4.4



Kidney
2.2
4.0



Kidney (fetal)
8.4
14.9



Renal ca. 786-0
7.6
2.5



Renal ca. A498
10.7
4.3



Renal ca. RXF 393
2.1
0.0



Renal ca. ACHN
0.9
0.0



Renal ca. UO-31
5.3
0.0



Renal ca. TK-10
4.5
1.8



Liver
5.3
9.5



Liver (fetal)
21.8
39.0



Liver ca. (hepatoblast) HepG2
19.5
9.7



Lung
38.7
53.6



Lung (fetal)
100.0
100.0



Lung ca. (small cell) LX-1
2.8
2.3



Lung ca. (small cell) NCI-H69
0.0
0.0



Lung ca. (s. cell var.) SHP-77
0.0
0.0



Lung ca. (large cell)NCI-H460
75.3
38.4



Lung ca. (non-sm. cell) A549
12.2
10.0



Lung ca. (non-s. cell) NCI-H23
2.3
2.3



Lung ca. (non-s. cell) HOP-62
2.2
0.6



Lung ca. (non-s. cl) NCI-H522
0.0
0.0



Lung ca. (squam.) SW 900
9.3
12.0



Lung ca. (squam.) NCI-H596
0.0
0.0



Mammary gland
3.8
1.9



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
15.5
10.4



Breast ca.* (pl. ef) T47D
0.0
0.0



Breast ca. BT-549
10.2
6.2



Breast ca. MDA-N
0.0
0.0



Ovary
13.1
7.7



Ovarian ca. OVCAR-3
1.7
0.6



Ovarian ca. OVCAR-4
0.1
0.0



Ovarian ca. OVCAR-5
7.3
1.2



Ovarian ca. OVCAR-8
2.4
0.0



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca.* (ascites) SK-OV-3
9.7
4.0



Uterus
9.6
6.3



Placenta
34.2
37.6



Prostate
12.9
12.6



Prostate ca.* (bone met)PC-3
1.2
0.8



Testis
39.5
13.0



Melanoma Hs688(A).T
0.0
0.0



Melanoma* (met) Hs688(B).T
0.3
0.0



Melanoma UACC-62
0.0
0.0



Melanoma M14
0.0
0.0



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0



Adipose
3.9
3.4









Column A - Rel. Exp. (%) Ag2775, Run 153780557






Column B - Rel. Exp. (%) Ag2776, Run 153792445














TABLE RG










Panel 2D











Tissue Name
A
B















Normal Colon
94.0
62.4



CC Well to Mod Diff (ODO3866)
5.0
3.1



CC Margin (ODO3866)
21.6
18.8



CC Gr.2 rectosigmoid (ODO3868)
4.5
2.0



CC Margin (ODO3868)
1.4
1.2



CC Mod Diff (ODO3920)
8.2
4.9



CC Margin (ODO3920)
34.4
28.5



CC Gr.2 ascend colon (ODO3921)
20.0
17.7



CC Margin (ODO3921)
12.2
10.2



CC from Partial Hepatectomy
11.3
4.8



(ODO4309) Mets



Liver Margin (ODO4309)
10.2
5.6



Colon mets to lung (OD04451-01)
4.7
0.8



Lung Margin (OD04451-02)
14.2
6.4



Normal Prostate 6546-1
7.6
5.4



Prostate Cancer (OD04410)
2.9
2.7



Prostate Margin (OD04410)
7.0
3.4



Prostate Cancer (OD04720-01)
7.0
4.0



Prostate Margin (OD04720-02)
8.1
6.5



Normal Lung 061010
44.1
45.4



Lung Met to Muscle (ODO4286)
4.3
5.2



Muscle Margin (ODO4286)
1.5
0.6



Lung Malignant Cancer (OD03126)
12.3
5.6



Lung Margin (OD03126)
33.7
13.8



Lung Cancer (OD04404)
4.1
0.9



Lung Margin (OD04404)
11.5
3.0



Lung Cancer (OD04565)
2.9
0.6



Lung Margin (OD04565)
19.6
3.7



Lung Cancer (OD04237-01)
6.8
3.4



Lung Margin (OD04237-02)
22.4
9.3



Ocular Mel Met to Liver (ODO4310)
11.3
5.8



Liver Margin (ODO4310)
10.4
6.0



Melanoma Mets to Lung (OD04321)
0.3
0.0



Lung Margin (OD04321)
27.9
31.6



Normal Kidney
7.0
3.6



Kidney Ca, Nuclear grade 2 (OD04338)
1.7
0.4



Kidney Margin (OD04338)
6.0
0.5



Kidney Ca Nuclear grade 1/2 (OD04339)
4.7
0.4



Kidney Margin (OD04339)
11.4
1.8



Kidney Ca, Clear cell type (OD04340)
0.9
0.0



Kidney Margin (OD04340)
10.6
3.5



Kidney Ca, Nuclear grade 3 (OD04348)
0.6
0.2



Kidney Margin (OD04348)
6.4
1.6



Kidney Cancer (OD04622-01)
0.0
0.0



Kidney Margin (OD04622-03)
0.9
0.2



Kidney Cancer (OD04450-01)
4.1
1.5



Kidney Margin (OD04450-03)
5.6
1.6



Kidney Cancer 8120607
0.0
0.0



Kidney Margin 8120608
0.1
0.0



Kidney Cancer 8120613
1.3
0.2



Kidney Margin 8120614
0.3
0.0



Kidney Cancer 9010320
1.5
0.0



Kidney Margin 9010321
1.0
0.0



Normal Uterus
1.8
0.8



Uterus Cancer 064011
4.6
1.4



Normal Thyroid
3.6
1.6



Thyroid Cancer 064010
1.7
1.1



Thyroid Cancer A302152
0.1
0.2



Thyroid Margin A302153
2.9
1.1



Normal Breast
2.8
1.2



Breast Cancer (OD04566)
2.9
0.2



Breast Cancer (OD04590-01)
1.5
0.5



Breast Cancer Mets (OD04590-03)
4.8
1.5



Breast Cancer Metastasis
0.7
0.4



(OD04655-05)



Breast Cancer 064006
2.9
1.4



Breast Cancer 1024
0.4
0.0



Breast Cancer 9100266
0.4
0.0



Breast Margin 9100265
0.1
0.2



Breast Cancer A209073
1.4
0.8



Breast Margin A209073
1.5
1.0



Normal Liver
7.7
1.7



Liver Cancer 064003
8.7
6.6



Liver Cancer 1025
3.3
0.8



Liver Cancer 1026
0.8
0.3



Liver Cancer 6004-T
3.8
0.7



Liver Tissue 6004-N
4.7
0.7



Liver Cancer 6005-T
0.8
0.2



Liver Tissue 6005-N
0.4
0.3



Normal Bladder
33.7
27.2



Bladder Cancer 1023
5.1
3.1



Bladder Cancer A302173
1.3
0.6



Bladder Cancer (OD04718-01)
12.2
9.6



Bladder Normal Adjacent (OD04718-03)
0.6
0.5



Normal Ovary
2.0
1.5



Ovarian Cancer 064008
5.8
4.0



Ovarian Cancer (OD04768-07)
0.1
0.0



Ovary Margin (OD04768-08)
0.2
0.1



Normal Stomach
25.9
12.3



Gastric Cancer 9060358
1.5
0.9



Stomach Margin 9060359
4.2
6.3



Gastric Cancer 9060395
14.6
9.8



Stomach Margin 9060394
8.7
12.4



Gastric Cancer 9060397
10.9
13.5



Stomach Margin 9060396
4.8
3.3



Gastric Cancer 064005
100.0
100.0









Column A - Rel. Exp. (%) Ag2775, Run 153780568






Column B - Rel. Exp. (%) Ag2776, Run 153792446














TABLE RH










Panel 4D










Tissue Name
A
B
C













Secondary Th1 act
0.0
0.0
0.0


Secondary Th2 act
0.4
0.0
0.6


Secondary Tr1 act
0.0
0.0
0.5


Secondary Th1 rest
0.2
0.0
0.0


Secondary Th2 rest
0.0
0.0
0.2


Secondary Tr1 rest
0.0
0.0
0.0


Primary Th1 act
0.0
0.0
0.0


Primary Th2 act
0.0
0.0
0.2


Primary Tr1 act
0.2
0.2
0.0


Primary Th1 rest
0.0
0.1
0.0


Primary Th2 rest
0.0
0.0
0.2


Primary Tr1 rest
0.1
0.0
0.0


CD45RA CD4 lymphocyte act
0.3
0.8
0.3


CD45RO CD4 lymphocyte act
0.0
0.0
0.3


CD8 lymphocyte act
0.3
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0
0.2


CD4 lymphocyte none
0.0
0.2
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0
0.3


LAK cells rest
0.0
0.1
0.8


LAK cells IL-2
0.7
0.0
0.0


LAK cells IL-2 + IL-12
0.2
0.4
0.0


LAK cells IL-2 + IFN gamma
0.9
0.4
0.9


LAK cells IL-2 + IL-18
1.0
0.0
0.6


LAK cells PMA/ionomycin
0.6
0.0
0.0


NK Cells IL-2 rest
0.0
0.0
0.0


Two Way MLR 3 day
0.2
0.4
0.4


Two Way MLR 5 day
0.0
0.0
0.0


Two Way MLR 7 day
0.0
0.0
0.0


PBMC rest
0.0
0.0
0.0


PBMC PWM
0.6
0.0
0.7


PBMC PHA-L
0.0
0.4
0.0


Ramos (B cell) none
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0


B lymphocytes PWM
0.6
0.4
0.6


B lymphocytes CD40L and IL-4
0.0
0.0
0.0


EOL-1 dbcAMP
0.2
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0


Dendritic cells none
0.0
0.0
0.0


Dendritic cells LPS
0.4
0.0
0.0


Dendritic cells anti-CD40
0.0
0.0
0.0


Monocytes rest
0.0
0.0
0.0


Monocytes LPS
0.5
0.0
0.0


Macrophages rest I
0.0
0.4
0.0


Macrophages LPS
0.0
0.0
0.0


HUVEC none
10.8
8.4
5.8


HUVEC starved
20.7
12.9
7.1


HUVEC IL-1 beta
5.6
5.6
2.3


HUVEC IFN gamma
22.1
19.9
8.2


HUVEC TNF alpha + IFN gamma
8.4
8.8
9.2


HUVEC TNF alpha + IL4
5.3
5.4
3.4


HUVEC IL-11
2.8
3.1
2.1


Lung Microvascular EC none
25.5
11.7
15.1


Lung Microvascular EC
12.4
10.2
9.3


TNFalpha + IL-1beta


Microvascular Dermal EC none
23.3
16.5
28.5


Microsvasular Dermal EC
12.4
9.4
9.9


TNFalpha + IL-1beta


Bronchial epithelium TNFalpha +
18.7
2.9
2.3


IL1beta


Small airway epithelium none
9.5
6.2
8.1


Small airway epithelium
82.4
67.4
31.2


TNFalpha + IL-1 beta


Coronery artery SMC rest
20.0
10.7
4.6


Coronery artery SMC TNFalpha +
10.7
10.6
2.6


IL-1beta


Astrocytes rest
0.4
0.7
0.9


Astrocytes TNFalpha + IL-1beta
2.3
1.8
1.4


KU-812 (Basophil) rest
0.9
0.3
0.4


KU-812 (Basophil) PMA/ionomycin
4.5
4.5
2.9


CCD1106 (Keratinocytes) none
9.0
6.6
7.6


CCD1106 (Keratinocytes)
4.6
0.8
2.6


TNFalpha + IL-1beta


Liver cirrhosis
9.0
12.1
5.7


Lupus kidney
1.4
1.6
0.9


NCI-H292 none
36.3
29.5
27.9


NCI-H292 IL-4
76.8
77.4
60.3


NCI-H292 IL-9
72.7
67.4
39.2


NCI-H292 IL-13
35.1
34.2
12.9


NCI-H292 IFN gamma
100.0
100.0
59.9


HPAEC none
5.3
3.7
2.7


HPAEC TNF alpha + IL-1 beta
4.8
4.2
4.3


Lung fibroblast none
13.2
8.7
4.9


Lung fibroblast TNF alpha + IL-1
30.8
28.9
20.9


beta


Lung fibroblast IL-4
22.4
18.7
13.8


Lung fibroblast IL-9
12.4
9.2
11.6


Lung fibroblast IL-13
11.4
8.0
13.9


Lung fibroblast IFN gamma
88.9
78.5
97.3


Dermal fibroblast CCD1070 rest
2.3
3.3
2.6


Dermal fibroblast CCD1070 TNF alpha
3.6
3.3
3.2


Dermal fibroblast CCD1070 IL-1 beta
0.8
0.8
0.5


Dermal fibroblast IFN gamma
0.2
0.2
0.0


Dermal fibroblast IL-4
0.7
0.0
0.0


IBD Colitis 2
0.2
1.4
0.0


IBD Crohn's
9.8
17.6
10.5


Colon
83.5
72.2
100.0


Lung
40.3
27.4
44.4


Thymus
21.6
18.4
23.8


Kidney
2.2
2.1
1.7







Column A - Rel. Exp. (%) Ag1920, Run 158535362





Column B - Rel. Exp. (%) Ag2775, Run 153780789





Column C - Rel. Exp. (%) Ag2776, Run 153792447







General_screening13 panel_v1.5 Summary: Ag5232 Highest expression was seen in fetal lung (CT=29.3), with moderate levels of expression also seen in breast, ovarian, lung and gastric cancer cell lines.


Panel 1.3D Summary: Ag2775/Ag2776 Expression of this gene was highest in fetal lung. This gene was expressed at higher levels in normal tissues than in the cancer cell lines. Significant expression of this gene was seen throughout the brain as well as in liver, small intestine, placenta, and bladder.


Panel 2D Summary: Ag2775/Ag2776 Expression of this gene was highest in a gastric cancer sample. Expression of this gene was lower in lung and kidney tumors when compared to the matched normal adjacent tissue. These results were consistent with what is observed in Panel 1.3D. Targeting of this gene or gene product through the use of protein, antibody, or small molecule therapeutics is beneficial in the treatment of lung and kidney cancer.


Panel 4D Summary: Ag1920/Ag2775/Ag2776 Expression of this gene was highest in lung cells, including in lung fibroblasts, small airway epithelium and the NCI-H292 cell line, a human airway epithelial cell line that produces mucins. These results were consistent with what is observed in Panels 1.3D and 2D. The expression of this gene in cells derived from or within the lung shows that this gene is involved in normal conditions as well as pathological and inflammatory lung disorders that include chronic obstructive pulmonary disease, asthma, allergy and emphysema.


Moderate expression of this gene was also seen in colon and thymus.


S. CG52171-04: Hematopoietic PBX-interacting Protein


Expression of gene CG52171-04 was assessed using the primer-probe set Ag2779, described in Table SA. Results of the RTQ-PCR runs are shown in Tables SB, SC, SD, SE and SF.

TABLE SAProbe Name Ag2779PrimersSequencesLengthStart PositionSEQ ID NoForward5′-caacagacaggtgatgatgatg-3′2220971336ProbeTET-5′-tgactttgaggacttcatcttcagcca-3′-TAMRA2721261337Reverse5′-cagtgctttgtctccaaagaag-3′2221531338









TABLE SB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
32.8



110980 COPD-F
28.7



110968 COPD-M
45.4



110977 COPD-M
70.7



110989 Emphysema-F
73.7



110992 Emphysema-F
25.7



110993 Emphysema-F
27.2



110994 Emphysema-F
14.9



110995 Emphysema-F
43.5



110996 Emphysema-F
10.7



110997 Asthma-M
8.7



111001 Asthma-F
25.0



111002 Asthma-F
33.9



111003 Atopic Asthma-F
27.2



111004 Atopic Asthma-F
43.8



111005 Atopic Asthma-F
27.4



111006 Atopic Asthma-F
7.3



111417 Allergy-M
1.2



112347 Allergy-M
2.9



112349 Normal Lung-F
2.4



112357 Normal Lung-F
100.0



112354 Normal Lung-M
32.1



112374 Crohns-F
24.1



112389 Match Control Crohns-F
17.7



112375 Crohns-F
25.9



112732 Match Control Crohns-F
39.5



112725 Crohns-M
3.0



112387 Match Control Crohns-M
31.6



112378 Crohns-M
2.3



112390 Match Control Crohns-M
45.7



112726 Crohns-M
45.4



112731 Match Control Crohns-M
29.5



112380 Ulcer Col-F
27.4



112734 Match Control Ulcer Col-F
57.8



112384 Ulcer Col-F
35.4



112737 Match Control Ulcer Col-F
11.6



112386 Ulcer Col-F
19.3



112738 Match Control Ulcer Col-F
13.3



112381 Ulcer Col-M
3.0



112735 Match Control Ulcer Col-M
11.0



112382 Ulcer Col-M
14.8



112394 Match Control Ulcer Col-M
8.5



112383 Ulcer Col-M
26.1



112736 Match Control Ulcer Col-M
9.9



112423 Psoriasis-F
18.3



112427 Match Control Psoriasis-F
71.7



112418 Psoriasis-M
28.1



112723 Match Control Psoriasis-M
35.6



112419 Psoriasis-M
45.1



112424 Match Control Psoriasis-M
15.7



112420 Psoriasis-M
62.0



112425 Match Control Psoriasis-M
40.1



104689 (MF) OA Bone-Backus
31.2



104690 (MF) Adj “Normal” Bone-Backus
21.0



104691 (MF) OA Synovium-Backus
41.5



104692 (BA) OA Cartilage-Backus
64.2



104694 (BA) OA Bone-Backus
28.5



104695 (BA) Adj “Normal” Bone-Backus
35.1



104696 (BA) OA Synovium-Backus
28.7



104700 (SS) OA Bone-Backus
18.8



104701 (SS) Adj “Normal” Bone-Backus
29.5



104702 (SS) OA Synovium-Backus
62.9



117093 OA Cartilage Rep7
46.7



112672 OA Bone5
74.7



112673 OA Synovium5
28.9



112674 OA Synovial Fluid cells5
28.5



117100 OA Cartilage Rep14
20.7



112756 OA Bone9
16.3



112757 OA Synovium9
45.1



112758 OA Synovial Fluid Cells9
14.4



117125 RA Cartilage Rep2
77.9



113492 Bone2 RA
24.8



113493 Synovium2 RA
8.1



113494 Syn Fluid Cells RA
14.3



113499 Cartilage4 RA
18.3



113500 Bone4 RA
22.5



113501 Synovium4 RA
14.1



113502 Syn Fluid Cells4 RA
10.7



113495 Cartilage3 RA
17.0



113496 Bone3 RA
19.8



113497 Synovium3 RA
11.9



113498 Syn Fluid Cells3 RA
28.1



117106 Normal Cartilage Rep20
50.0



113663 Bone3 Normal
3.3



113664 Synovium3 Normal
1.1



113665 Syn Fluid Cells3 Normal
2.0



117107 Normal Cartilage Rep22
6.4



113667 Bone4 Normal
21.6



113668 Synovium4 Normal
24.8



113669 Syn Fluid Cells4 Normal
48.6









Column A - Rel. Exp. (%) Ag2779, Run 249247310














TABLE SC










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
3.5



Pancreas
1.2



Pancreatic ca. CAPAN 2
0.3



Adrenal gland
3.7



Thyroid
12.1



Salivary gland
2.1



Pituitary gland
14.9



Brain (fetal)
0.6



Brain (whole)
4.0



Brain (amygdala)
7.0



Brain (cerebellum)
2.1



Brain (hippocampus)
11.0



Brain (substantia nigra)
2.5



Brain (thalamus)
6.4



Cerebral Cortex
10.0



Spinal cord
17.2



glio/astro U87-MG
2.8



glio/astro U-118-MG
1.6



astrocytoma SW1783
2.9



neuro*; met SK-N-AS
2.2



astrocytoma SF-539
3.3



astrocytoma SNB-75
2.6



glioma SNB-19
3.2



glioma U251
2.5



glioma SF-295
4.6



Heart (fetal)
15.8



Heart
4.1



Skeletal muscle (fetal)
100.0



Skeletal muscle
24.8



Bone marrow
1.7



Thymus
30.8



Spleen
11.3



Lymph node
5.9



Colorectal
8.5



Stomach
4.2



Small intestine
7.7



Colon ca. SW480
0.5



Colon ca.* SW620(SW480 met)
0.4



Colon ca. HT29
1.5



Colon ca. HCT-116
0.3



Colon ca. CaCo-2
3.0



Colon ca. tissue(ODO3866)
2.8



Colon ca. HCC-2998
0.9



Gastric ca.* (liver met) NCI-N87
1.6



Bladder
3.3



Trachea
11.8



Kidney
9.2



Kidney (fetal)
6.4



Renal ca. 786-0
1.8



Renal ca. A498
3.3



Renal ca. RXF 393
1.6



Renal ca. ACHN
2.2



Renal ca. UO-31
1.2



Renal ca. TK-10
1.0



Liver
1.0



Liver (fetal)
2.7



Liver ca. (hepatoblast) HepG2
1.4



Lung
6.4



Lung (fetal)
4.9



Lung ca. (small cell) LX-1
0.4



Lung ca. (small cell) NCI-H69
0.2



Lung ca. (s. cell var.) SHP-77
1.6



Lung ca. (large cell)NCI-H460
0.6



Lung ca. (non-sm. cell) A549
0.6



Lung ca. (non-s. cell) NCI-H23
4.8



Lung ca. (non-s. cell) HOP-62
4.3



Lung ca. (non-s. cl) NCI-H522
0.5



Lung ca. (squam.) SW 900
3.5



Lung ca. (squam.) NCI-H596
0.2



Mammary gland
5.1



Breast ca.* (pl. ef) MCF-7
3.3



Breast ca.* (pl. ef) MDA-MB-231
0.9



Breast ca.* (pl. ef) T47D
3.2



Breast ca. BT-549
2.2



Breast ca. MDA-N
0.5



Ovary
40.1



Ovarian ca. OVCAR-3
2.5



Ovarian ca. OVCAR-4
0.4



Ovarian ca. OVCAR-5
1.7



Ovarian ca. OVCAR-8
1.3



Ovarian ca. IGROV-1
1.1



Ovarian ca.* (ascites) SK-OV-3
0.5



Uterus
4.6



Placenta
5.1



Prostate
5.4



Prostate ca.* (bone met)PC-3
1.6



Testis
8.6



Melanoma Hs688(A).T
1.5



Melanoma* (met) Hs688(B).T
2.3



Melanoma UACC-62
0.8



Melanoma M14
0.4



Melanoma LOX IMVI
0.1



Melanoma* (met) SK-MEL-5
1.3



Adipose
6.4









Column A - Rel. Exp. (%) Ag2779, Run 164024255














TABLE SD










Panel 2D










Tissue Name
A














Normal Colon
33.4



CC Well to Mod Diff (ODO3866)
4.2



CC Margin (ODO3866)
10.7



CC Gr.2 rectosigmoid (ODO3868)
6.0



CC Margin (ODO3868)
7.5



CC Mod Diff (ODO3920)
5.8



CC Margin (ODO3920)
18.2



CC Gr.2 ascend colon (ODO3921)
15.9



CC Margin (ODO3921)
9.9



CC from Partial Hepatectomy (ODO4309) Mets
22.4



Liver Margin (ODO4309)
9.9



Colon mets to lung (OD04451-01)
15.1



Lung Margin (OD04451-02)
11.7



Normal Prostate 6546-1
100.0



Prostate Cancer (OD04410)
14.0



Prostate Margin (OD04410)
30.8



Prostate Cancer (OD04720-01)
25.0



Prostate Margin (OD04720-02)
28.9



Normal Lung 061010
30.6



Lung Met to Muscle (ODO4286)
12.2



Muscle Margin (ODO4286)
52.9



Lung Malignant Cancer (OD03126)
71.2



Lung Margin (OD03126)
23.0



Lung Cancer (OD04404)
11.9



Lung Margin (OD04404)
22.8



Lung Cancer (OD04565)
17.8



Lung Margin (OD04565)
15.4



Lung Cancer (OD04237-01)
24.0



Lung Margin (OD04237-02)
16.0



Ocular Mel Met to Liver (ODO4310)
13.8



Liver Margin (ODO4310)
9.8



Melanoma Mets to Lung (OD04321)
4.4



Lung Margin (OD04321)
25.9



Normal Kidney
31.6



Kidney Ca, Nuclear grade 2 (OD04338)
53.2



Kidney Margin (OD04338)
23.8



Kidney Ca Nuclear grade 1/2 (OD04339)
52.1



Kidney Margin (OD04339)
32.8



Kidney Ca, Clear cell type (OD04340)
40.6



Kidney Margin (OD04340)
33.7



Kidney Ca, Nuclear grade 3 (OD04348)
10.7



Kidney Margin (OD04348)
16.0



Kidney Cancer (OD04622-01)
32.5



Kidney Margin (OD04622-03)
10.9



Kidney Cancer (OD04450-01)
27.5



Kidney Margin (OD04450-03)
25.0



Kidney Cancer 8120607
17.4



Kidney Margin 8120608
23.3



Kidney Cancer 8120613
47.3



Kidney Margin 8120614
15.6



Kidney Cancer 9010320
15.2



Kidney Margin 9010321
32.1



Normal Uterus
13.5



Uterus Cancer 064011
21.2



Normal Thyroid
41.2



Thyroid Cancer 064010
20.0



Thyroid Cancer A302152
71.2



Thyroid Margin A302153
32.5



Normal Breast
28.7



Breast Cancer (OD04566)
7.3



Breast Cancer (OD04590-01)
17.9



Breast Cancer Mets (OD04590-03)
27.5



Breast Cancer Metastasis (OD04655-05)
20.7



Breast Cancer 064006
22.7



Breast Cancer 1024
29.5



Breast Cancer 9100266
32.5



Breast Margin 9100265
17.9



Breast Cancer A209073
21.8



Breast Margin A209073
11.5



Normal Liver
6.5



Liver Cancer 064003
6.3



Liver Cancer 1025
8.1



Liver Cancer 1026
13.6



Liver Cancer 6004-T
7.0



Liver Tissue 6004-N
12.2



Liver Cancer 6005-T
10.6



Liver Tissue 6005-N
4.0



Normal Bladder
15.4



Bladder Cancer 1023
6.6



Bladder Cancer A302173
4.9



Bladder Cancer (OD04718-01)
11.2



Bladder Normal Adjacent (OD04718-03)
28.5



Normal Ovary
32.1



Ovarian Cancer 064008
26.8



Ovarian Cancer (OD04768-07)
37.4



Ovary Margin (OD04768-08)
16.7



Normal Stomach
35.6



Gastric Cancer 9060358
9.1



Stomach Margin 9060359
24.8



Gastric Cancer 9060395
28.3



Stomach Margin 9060394
26.4



Gastric Cancer 9060397
15.8



Stomach Margin 9060396
10.6



Gastric Cancer 064005
21.0









Column A - Rel. Exp. (%) Ag2779, Run 162555295














TABLE SE










Panel 4D










Tissue Name
A














Secondary Th1 act
13.4



Secondary Th2 act
8.4



Secondary Tr1 act
11.0



Secondary Th1 rest
37.1



Secondary Th2 rest
49.0



Secondary Tr1 rest
49.3



Primary Th1 act
4.7



Primary Th2 act
1.9



Primary Tr1 act
9.0



Primary Th1 rest
91.4



Primary Th2 rest
81.8



Primary Tr1 rest
35.6



CD45RA CD4 lymphocyte act
4.1



CD45RO CD4 lymphocyte act
15.6



CD8 lymphocyte act
11.9



Secondary CD8 lymphocyte rest
9.7



Secondary CD8 lymphocyte act
8.6



CD4 lymphocyte none
49.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
89.5



LAK cells rest
17.3



LAK cells IL-2
36.1



LAK cells IL-2 + IL-12
17.1



LAK cells IL-2 + IFN gamma
28.7



LAK cells IL-2 + IL-18
23.5



LAK cells PMA/ionomycin
4.9



NK Cells IL-2 rest
26.2



Two Way MLR 3 day
31.9



Two Way MLR 5 day
15.4



Two Way MLR 7 day
27.4



PBMC rest
39.8



PBMC PWM
29.5



PBMC PHA-L
16.5



Ramos (B cell) none
5.8



Ramos (B cell) ionomycin
14.1



B lymphocytes PWM
25.9



B lymphocytes CD40L and IL-4
36.1



EOL-1 dbcAMP
9.8



EOL-1 dbcAMP PMA/ionomycin
4.7



Dendritic cells none
12.1



Dendritic cells LPS
9.9



Dendritic cells anti-CD40
9.0



Monocytes rest
12.9



Monocytes LPS
3.7



Macrophages rest
10.2



Macrophages LPS
5.5



HUVEC none
17.1



HUVEC starved
29.3



HUVEC IL-1beta
4.3



HUVEC IFN gamma
23.0



HUVEC TNF alpha + IFN gamma
4.2



HUVEC TNF alpha + IL4
7.1



HUVEC IL-11
18.4



Lung Microvascular EC none
18.2



Lung Microvascular EC TNFalpha + IL-1beta
15.1



Microvascular Dermal EC none
29.5



Microsvasular Dermal EC TNFalpha + IL-1beta
9.4



Bronchial epithelium TNFalpha + IL1beta
20.4



Small airway epithelium none
5.0



Small airway epithelium TNFalpha + IL-1beta
21.0



Coronery artery SMC rest
24.3



Coronery artery SMC TNFalpha + IL-1beta
18.3



Astrocytes rest
34.2



Astrocytes TNFalpha + IL-1beta
29.1



KU-812 (Basophil) rest
5.8



KU-812 (Basophil) PMA/ionomycin
10.5



CCD1106 (Keratinocytes) none
4.7



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
4.3



Liver cirrhosis
7.6



Lupus kidney
11.5



NCI-H292 none
15.3



NCI-H292 IL-4
8.9



NCI-H292 IL-9
11.0



NCI-H292 IL-13
6.3



NCI-H292 IFN gamma
6.0



HPAEC none
26.2



HPAEC TNF alpha + IL-1 beta
11.5



Lung fibroblast none
27.4



Lung fibroblast TNF alpha + IL-1 beta
66.0



Lung fibroblast IL-4
25.2



Lung fibroblast IL-9
22.4



Lung fibroblast IL-13
18.3



Lung fibroblast IFN gamma
34.9



Dermal fibroblast CCD1070 rest
8.8



Dermal fibroblast CCD1070 TNF alpha
49.3



Dermal fibroblast CCD1070 IL-1 beta
4.9



Dermal fibroblast IFN gamma
4.9



Dermal fibroblast IL-4
11.2



IBD Colitis 2
5.1



IBD Crohn's
11.1



Colon
42.6



Lung
26.8



Thymus
41.5



Kidney
100.0









Column A - Rel. Exp. (%) Ag2779, Run 162188258














TABLE SF










Panel 5D










Tissue Name
A














97457_Patient-02go_adipose
30.1



97476_Patient-07sk_skeletal muscle
13.8



97477_Patient-07ut_uterus
31.0



97478_Patient-07pl_placenta
17.4



97481_Patient-08sk_skeletal muscle
38.7



97482_Patient-08ut_uterus
45.4



97483_Patient-08pl_placenta
16.0



97486_Patient-09sk_skeletal muscle
7.4



97487_Patient-09ut_uterus
19.8



97488_Patient-09pl_placenta
16.7



97492_Patient-10ut_uterus
66.4



97493_Patient-10pl_placenta
46.3



97495_Patient-11go_adipose
7.2



97496_Patient-11sk_skeletal muscle
12.4



97497_Patient-11ut_uterus
68.3



97498_Patient-11pl_placenta
26.4



97500_Patient-12go_adipose
35.8



97501_Patient-12sk_skeletal muscle
78.5



97502_Patient-12ut_uterus
100.0



97503_Patient-12pl_placenta
21.5



94721_Donor 2 U - A_Mesenchymal Stem Cells
23.3



94722_Donor 2 U - B_Mesenchymal Stem Cells
28.1



94723_Donor 2 U - C_Mesenchymal Stem Cells
20.0



94709_Donor 2 AM - A_adipose
16.0



94710_Donor 2 AM - B_adipose
15.1



94711_Donor 2 AM - C_adipose
7.9



94712_Donor 2 AD - A_adipose
32.1



94713_Donor 2 AD - B_adipose
40.1



94714_Donor 2 AD - C_adipose
35.1



94742_Donor 3 U - A_Mesenchymal Stem Cells
10.1



94743_Donor 3 U - B_Mesenchymal Stem Cells
14.1



94730_Donor 3 AM - A_adipose
13.5



94731_Donor 3 AM - B_adipose
8.8



94732_Donor 3 AM - C_adipose
5.2



94733_Donor 3 AD - A_adipose
26.6



94734_Donor 3 AD - B_adipose
17.4



94735_Donor 3 AD - C_adipose
26.1



77138_Liver_HepG2untreated
25.0



73556_Heart_Cardiac stromal cells (primary)
7.3



81735_Small Intestine
55.5



72409_Kidney_Proximal Convoluted Tubule
4.2



82685_Small intestine_Duodenum
21.2



90650_Adrenal_Adrenocortical adenoma
30.1



72410_Kidney_HRCE
25.7



72411_Kidney_HRE
14.3



73139_Uterus_Uterine smooth muscle cells
14.7









Column A - Rel. Exp. (%) Ag2779, Run 255179443







AI_comprehensive panel_v1.0 Summary: Ag2779 Highest expression of this gene was detected in normal lung (CT=27). Moderate levels of expression of this gene were detected in samples derived from osteoarthritic (OA) bone and normal adjacent bone as well as OA cartilage, OA synovium and OA synovial fluid samples. Moderate to low levels of expression were also detected in cartilage, bone, synovium and synovial fluid samples from rheumatoid arthritis patients as well as in samples derived from normal lung samples, COPD lung, emphysema, atopic asthma, asthma, allergy, Crohn's disease (normal matched control and diseased), ulcerative colitis(normal matched control and diseased), and psoriasis (normal matched control and diseased). Therapeutic modulation of this gene or gene product is useful for the amelioration of symptoms/conditions associated with autoimmune and inflammatory disorders including psoriasis, allergy, asthma, inflammatory bowel disease, rheumatoid arthritis and osteoarthritis.


Panel 1.3D Summary: Ag2779 Highest expression was seen in fetal skeletal muscle (CT=25). Prominent expression was seen in normal tissues including ovary and bone marrow.


Panel 2D Summary: Ag2779 Highest expression was seen in prostate (CT=23.5). High levels of expression were seen in most of the samples on this panel, showing that this gene or its protein product plays a role in cell growth and/or proliferation.


Panel 4D Summary: Ag2779 Highest expression was seen in the kidney (CT=26.7). In addition, this gene was more highly expressed in resting T cells (Th1, Th2, Tr1) than in activated T cells. Thus, this gene is be involved in T lymphocyte function. Therapeutics targeting this gene or its protein product are useful for the stimulation of their activity and act as anti-inflammatory therapeutic for T cell-mediated autoimmune and inflammatory diseases.


Panel SD Summary: Ag2779 Highest expression was seen in normal uterus (CT=27), with prominent expression in normal adipose and placenta. This strong expression in normal tissues was in agreement with the results in Panel 1.3D.


T. CG52979-03 and CG52988-02 and CG52988-03: GAGE


Expression of gene CG52979-03, variant CG52988-02 and full-length physical clone CG52988-03 was assessed using the primer-probe sets Ag436b, and Ag436, described in Tables TA and TB. Results of the RTQ-PCR runs are shown in Tables TC, TD, TE, TF, TG and TH.

TABLE TAProbe Name Ag436bPrimersSequencesLengthStart PositionSEQ ID NoForward5′-gagcctggagatggtcctga-3′201841339ProbeTET-5′-agatcaaaagtgggcataataccctccctga-3′-TAMRA311511340Reverse5′-atcacctgcttccagcactttagt-3′241261341









TABLE TB










Probe Name Ag436











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-gcctggagatggtcctgatg-3′
20
182
1342






Probe
TET-5′-tgagatcaaaagtgggcataataccctccct-3′-TAMRA
31
149
1343





Reverse
5′-catcacctgcttccagcactt-3′
21
125
1344
















TABLE TC










Panel 1










Tissue Name
A














Endothelial cells
0.0



Endothelial cells (treated)
0.0



Pancreas
1.6



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
0.4



Thyroid
0.0



Salivary gland
0.1



Pituitary gland
0.0



Brain (fetal)
0.0



Brain (whole)
0.0



Brain (amygdala)
0.0



Brain (cerebellum)
0.0



Brain (hippocampus)
0.1



Brain (substantia nigra)
0.1



Brain (thalamus)
0.0



Brain (hypothalamus)
0.4



Spinal cord
0.3



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.9



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.3



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.1



glioma U251
0.1



glioma SF-295
4.5



Heart
0.1



Skeletal muscle
0.0



Bone marrow
6.1



Thymus
1.0



Spleen
0.8



Lymph node
1.4



Colon (ascending)
3.3



Stomach
0.3



Small intestine
0.2



Colon ca. SW480
0.1



Colon ca.* SW620 (SW480 met)
0.1



Colon ca. HT29
0.0



Colon ca. HCT-116
0.1



Colon ca. CaCo-2
0.0



Colon ca. HCT-15
0.2



Colon ca. HCC-2998
0.2



Gastric ca.* (liver met) NCI-N87
0.2



Bladder
0.0



Trachea
0.2



Kidney
0.2



Kidney (fetal)
0.3



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.4



Liver (fetal)
4.6



Liver ca. (hepatoblast) HepG2
0.3



Lung
0.0



Lung (fetal)
0.0



Lung ca. (small cell) LX-1
1.3



Lung ca. (small cell) NCI-H69
0.8



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
1.0



Lung ca. (non-sm. cell) A549
0.5



Lung ca. (non-s. cell) NCI-H23
1.0



Lung ca. (non-s. cell) HOP-62
0.2



Lung ca. (non-s. cl) NCI-H522
0.3



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.2



Mammary gland
1.3



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.5



Breast ca.* (pl. ef) T47D
0.5



Breast ca. BT-549
0.0



Breast ca. MDA-N
100.0



Ovary
0.0



Ovarian ca. OVCAR-3
1.3



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
3.5



Ovarian ca. IGROV-1
0.0



Ovarian ca. (ascites) SK-OV-3
0.0



Uterus
0.1



Placenta
13.3



Prostate
0.4



Prostate ca.* (bone met) PC-3
0.9



Testis
79.0



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
0.1



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Melanoma SK-MEL-28
8.7









Column A - Rel. Exp. (%) Ag436, Run 109664430














TABLE TD










Panel 1.1











Tissue Name
A
B















Adrenal gland
0.0
0.0



Bladder
0.1
0.0



Brain (amygdala)
0.0
0.0



Brain (cerebellum)
0.4
0.0



Brain (hippocampus)
0.0
0.0



Brain (substantia nigra)
0.4
0.0



Brain (thalamus)
0.0
0.0



Cerebral Cortex
0.0
0.0



Brain (fetal)
0.0
0.0



Brain (whole)
0.4
0.0



glio/astro U-118-MG
0.8
0.1



astrocytoma SF-539
0.0
0.0



astrocytoma SNB-75
0.0
0.0



astrocytoma SW1783
0.0
0.0



glioma U251
0.1
0.0



glioma SF-295
3.3
4.2



glioma SNB-19
0.2
0.0



glio/astro U87-MG
0.0
0.0



neuro*; met SK-N-AS
0.2
0.0



Mammary gland
0.1
0.0



Breast ca. BT-549
0.0
0.0



Breast ca. MDA-N
100.0
100.0



Breast ca.* (pl. ef) T47D
0.4
0.0



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0



Small intestine
0.3
0.0



Colorectal
0.0
0.0



Colon ca. HT29
0.3
0.0



Colon ca. CaCo-2
0.1
0.0



Colon ca. HCT-15
0.1
0.0



Colon ca. HCT-116
0.1
0.0



Colon ca. HCC-2998
0.2
0.0



Colon ca. SW480
0.1
0.0



Colon ca.* SW620 (SW480 met)
0.4
0.0



Stomach
0.4
0.0



Gastric ca. (liver met) NCI-N87
0.4
0.0



Heart
0.0
0.0



Skeletal muscle (Fetal)
0.0
0.0



Skeletal muscle
0.0
0.0



Endothelial cells
0.0
0.0



Heart (Fetal)
0.0
0.0



Kidney
0.3
0.0



Kidney (fetal)
0.2
0.0



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. TK-10
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. RXF 393
0.0
0.0



Liver
0.6
0.0



Liver (fetal)
1.5
1.7



Liver ca. (hepatoblast) HepG2
0.2
0.0



Lung
0.0
0.0



Lung (fetal)
0.3
0.0



Lung ca. (non-s. cell) HOP-62
0.4
0.2



Lung ca. (large cell)NCI-H460
0.3
0.0



Lung ca. (non-s. cell) NCI-H23
0.4
0.0



Lung ca. (non-s. cl) NCI-H522
1.0
0.3



Lung ca. (non-sm. cell) A549
0.2
0.0



Lung ca. (s. cell var.) SHP-77
0.2
0.0



Lung ca. (small cell) LX-1
2.3
0.0



Lung ca. (small cell) NCI-H69
0.9
0.0



Lung ca. (squam.) SW 900
0.0
0.0



Lung ca. (squam.) NCI-H596
0.2
0.0



Lymph node
0.4
0.0



Spleen
0.2
0.0



Thymus
0.4
0.0



Ovary
0.0
0.0



Ovarian ca. IGROV-1
0.1
0.0



Ovarian ca. OVCAR-3
0.9
0.3



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
0.4
0.0



Ovarian ca. OVCAR-8
0.9
0.2



Ovarian ca.* (ascites) SK-OV-3
0.0
0.0



Pancreas
2.4
5.1



Pancreatic ca. CAPAN 2
0.0
0.0



Pituitary gland
0.2
0.0



Placenta
4.2
3.8



Prostate
0.5
0.0



Prostate ca.* (bone met) PC-3
0.3
0.0



Salivary gland
0.2
0.0



Trachea
0.0
0.0



Spinal cord
0.2
0.0



Testis
44.4
28.7



Thyroid
0.0
0.0



Uterus
0.0
0.0



Melanoma M14
0.2
0.0



Melanoma LOX IMVI
0.0
0.0



Melanoma UACC-62
0.0
0.0



Melanoma SK-MEL-28
0.1
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0



Melanoma Hs688(A).T
0.0
0.0



Melanoma* (met) Hs688(B).T
0.0
0.0









Column A - Rel. Exp. (%) Ag436, Run 109665004






Column B - Rel. Exp. (%) Ag436b, Run 111165634














TABLE TE










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
2.5



Pancreas
0.8



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
0.0



Thyroid
0.5



Salivary gland
0.0



Pituitary gland
0.6



Brain (fetal)
3.5



Brain (whole)
1.6



Brain (amygdala)
0.0



Brain (cerebellum)
2.2



Brain (hippocampus)
2.9



Brain (substantia nigra)
2.1



Brain (thalamus)
0.7



Cerebral Cortex
1.4



Spinal cord
0.0



glio/astro U87-MG
1.0



glio/astro U-118-MG
5.1



astrocytoma SW1783
4.3



neuro*; met SK-N-AS
0.7



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.8



glioma U251
0.3



glioma SF-295
5.6



Heart (fetal)
0.8



Heart
0.0



Skeletal muscle (fetal)
0.0



Skeletal muscle
0.0



Bone marrow
16.7



Thymus
0.7



Spleen
1.4



Lymph node
3.7



Colorectal
1.1



Stomach
1.1



Small intestine
1.3



Colon ca. SW480
0.7



Colon ca.* SW620(SW480 met)
1.0



Colon ca. HT29
2.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.7



Colon ca. tissue(ODO3866)
0.0



Colon ca. HCC-2998
0.7



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.0



Trachea
0.0



Kidney
0.8



Kidney (fetal)
3.3



Renal ca. 786-0
1.0



Renal ca. A498
1.0



Renal ca. RXF 393
1.7



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
2.2



Liver (fetal)
11.6



Liver ca. (hepatoblast) HepG2
0.0



Lung
2.7



Lung (fetal)
4.0



Lung ca. (small cell) LX-1
2.5



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
8.4



Lung ca. (non-s. cell) NCI-H23
2.8



Lung ca. (non-s. cell) HOP-62
0.9



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
1.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
0.0



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
1.7



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
0.0



Breast ca. MDA-N
76.8



Ovary
0.0



Ovarian ca. OVCAR-3
3.1



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.8



Ovarian ca. OVCAR-8
2.5



Ovarian ca. IGROV-1
0.9



Ovarian ca.* (ascites) SK-OV-3
1.7



Uterus
0.0



Placenta
6.3



Prostate
1.3



Prostate ca.* (bone met)PC-3
1.7



Testis
100.0



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.9



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
2.0



Adipose
2.5









Column A - Rel. Exp. (%) Ag436, Run 153954656














TABLE TF










Panel 2D










Tissue Name
A














Normal Colon
0.0



CC Well to Mod Diff (ODO3866)
0.9



CC Margin (ODO3866)
0.9



CC Gr.2 rectosigmoid (ODO3868)
0.0



CC Margin (ODO3868)
0.8



CC Mod Diff (ODO3920)
0.5



CC Margin (ODO3920)
0.4



CC Gr.2 ascend colon (ODO3921)
0.9



CC Margin (ODO3921)
0.5



CC from Partial Hepatectomy (ODO4309) Mets
1.7



Liver Margin (ODO4309)
0.8



Colon mets to lung (OD04451-01)
0.8



Lung Margin (OD04451-02)
0.0



Normal Prostate 6546-1
1.4



Prostate Cancer (OD04410)
1.5



Prostate Margin (OD04410)
0.3



Prostate Cancer (OD04720-01)
1.6



Prostate Margin (OD04720-02)
1.0



Normal Lung 061010
1.4



Lung Met to Muscle (ODO4286)
0.6



Muscle Margin (ODO4286)
0.7



Lung Malignant Cancer (OD03126)
0.0



Lung Margin (OD03126)
0.6



Lung Cancer (OD04404)
0.0



Lung Margin (OD04404)
0.0



Lung Cancer (OD04565)
0.6



Lung Margin (OD04565)
0.7



Lung Cancer (OD04237-01)
0.9



Lung Margin (OD04237-02)
0.2



Ocular Mel Met to Liver (ODO4310)
0.0



Liver Margin (ODO4310)
0.2



Melanoma Mets to Lung (OD04321)
0.6



Lung Margin (OD04321)
0.4



Normal Kidney
1.0



Kidney Ca, Nuclear grade 2 (OD04338)
0.2



Kidney Margin (OD04338)
2.0



Kidney Ca Nuclear grade 1/2 (OD04339)
0.4



Kidney Margin (OD04339)
0.6



Kidney Ca, Clear cell type (OD04340)
0.4



Kidney Margin (OD04340)
0.2



Kidney Ca, Nuclear grade 3 (OD04348)
0.3



Kidney Margin (OD04348)
0.0



Kidney Cancer (OD04622-01)
0.3



Kidney Margin (OD04622-03)
0.0



Kidney Cancer (OD04450-01)
0.0



Kidney Margin (OD04450-03)
0.5



Kidney Cancer 8120607
0.0



Kidney Margin 8120608
0.2



Kidney Cancer 8120613
0.2



Kidney Margin 8120614
0.0



Kidney Cancer 9010320
56.6



Kidney Margin 9010321
0.0



Normal Uterus
0.0



Uterus Cancer 064011
0.7



Normal Thyroid
0.4



Thyroid Cancer 064010
0.0



Thyroid Cancer A302152
0.3



Thyroid Margin A302153
0.3



Normal Breast
0.4



Breast Cancer (OD04566)
1.3



Breast Cancer (OD04590-01)
1.1



Breast Cancer Mets (OD04590-03)
2.7



Breast Cancer Metastasis (OD04655-05)
3.5



Breast Cancer 064006
37.9



Breast Cancer 1024
1.1



Breast Cancer 9100266
0.4



Breast Margin 9100265
0.4



Breast Cancer A209073
1.0



Breast Margin A209073
0.9



Normal Liver
0.0



Liver Cancer 064003
0.3



Liver Cancer 1025
0.0



Liver Cancer 1026
0.0



Liver Cancer 6004-T
0.2



Liver Tissue 6004-N
1.0



Liver Cancer 6005-T
0.2



Liver Tissue 6005-N
0.6



Normal Bladder
0.3



Bladder Cancer 1023
0.0



Bladder Cancer A302173
100.0



Bladder Cancer (OD04718-01)
2.8



Bladder Normal Adjacent (OD04718-03)
0.6



Normal Ovary
0.0



Ovarian Cancer 064008
1.0



Ovarian Cancer (OD04768-07)
2.1



Ovary Margin (OD04768-08)
0.6



Normal Stomach
0.9



Gastric Cancer 9060358
0.3



Stomach Margin 9060359
0.8



Gastric Cancer 9060395
0.5



Stomach Margin 9060394
0.0



Gastric Cancer 9060397
1.4



Stomach Margin 9060396
0.0



Gastric Cancer 064005
0.3









Column A - Rel. Exp. (%) Ag436, Run 153954657














TABLE TG










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
0.0



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.1



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
0.2



SNB-78- Glioma
0.0



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
0.1



SK-N-SH- Neuroblastoma (metastasis)
0.5



SF-295- Glioblastoma
0.5



Cerebellum
0.2



Cerebellum
0.0



NCI-H292- Mucoepidermoid lung carcinoma
0.0



DMS-114- Small cell lung cancer
0.0



DMS-79- Small cell lung cancer
0.0



NCI-H146- Small cell lung cancer
16.0



NCI-H526- Small cell lung cancer
0.1



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
0.0



NCI-H157- Squamous cell lung cancer (metastasis)
0.0



NCI-H1155- Large cell lung cancer
0.0



NCI-H1299- Large cell lung cancer
7.6



NCI-H727- Lung carcinoid
0.1



NCI-UMC-11- Lung carcinoid
0.1



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
0.1



KM12- Colon cancer
0.0



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.1



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
0.0



KATO III- Gastric carcinoma
0.0



NCI-SNU-16- Gastric carcinoma
0.0



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.0



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
0.0



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma (metastasis)
0.0



ES-2- Ovarian clear cell carcinoma
0.0



Ramos- Stimulated with PMA/ionomycin 6 h
0.1



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
0.1



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
100.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
0.0



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
0.5



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
0.0



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
0.0



Hs766T- Pancreatic carcinoma (LN metastasis)
0.0



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver
0.0



metastasis)



BxPC-3- Pancreatic adenocarcinoma
0.0



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.0



CFPAC-1- Pancreatic ductal adenocarcinoma
0.1



PANC-1- Pancreatic epithelioid ductal
0.0



carcinoma



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
0.0



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
0.0



A204- Rhabdomyosarcoma
0.1



HT-1080- Fibrosarcoma
0.0



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
0.0



SJRH30- Rhabdomyosarcoma (met to bone marrow)
0.0



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
0.0



DU 145- Prostate carcinoma (brain metastasis)
0.1



MDA-MB-468- Breast adenocarcinoma
0.1



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.1



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp. (%) Ag436, Run 168238445














TABLE TH










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
18.6



Secondary Th1 rest
22.7



Secondary Th2 rest
22.5



Secondary Tr1 rest
6.8



Primary Th1 act
19.5



Primary Th2 act
22.1



Primary Tr1 act
35.8



Primary Th1 rest
47.6



Primary Th2 rest
62.0



Primary Tr1 rest
12.9



CD45RA CD4 lymphocyte act
16.8



CD45RO CD4 lymphocyte act
29.9



CD8 lymphocyte act
43.2



Secondary CD8 lymphocyte rest
9.9



Secondary CD8 lymphocyte act
34.4



CD4 lymphocyte none
24.5



2ry Th1/Th2/Tr1_anti-CD95 CH11
26.8



LAK cells rest
30.4



LAK cells IL-2
41.2



LAK cells IL-2 + IL-12
8.5



LAK cells IL-2 + IFN gamma
44.8



LAK cells IL-2 + IL-18
40.9



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
27.0



Two Way MLR 3 day
34.2



Two Way MLR 5 day
9.3



Two Way MLR 7 day
33.0



PBMC rest
0.0



PBMC PWM
62.4



PBMC PHA-L
54.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
17.6



B lymphocytes PWM
11.5



B lymphocytes CD40L and IL-4
10.6



EOL-1 dbcAMP
6.2



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
8.4



Dendritic cells LPS
11.1



Dendritic cells anti-CD40
7.6



Monocytes rest
0.0



Monocytes LPS
9.3



Macrophages rest
9.3



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
10.2



HUVEC IL-1beta
19.1



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
6.8



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
19.3



Lung Microvascular EC TNFalpha + IL-1beta
31.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
18.2



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
89.5



KU-812 (Basophil) PMA/ionomycin
95.3



CCD1106 (Keratinocytes) none
5.8



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
35.6



Lupus kidney
0.0



NCI-H292 none
8.9



NCI-H292 IL-4
22.5



NCI-H292 IL-9
39.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
16.6



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
21.2



Lung fibroblast TNF alpha + IL-1 beta
27.5



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
11.4



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
20.0



Dermal fibroblast CCD1070 rest
4.2



Dermal fibroblast CCD1070 TNF alpha
41.2



Dermal fibroblast CCD1070 IL-1 beta
11.3



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



IBD Colitis 2
10.1



IBD Crohn's
0.0



Colon
33.0



Lung
0.0



Thymus
28.7



Kidney
100.0









Column A - Rel. Exp. (%) Ag436, Run 153954658







Panel 1 Summary: Ag436 Expression of this gene was highest in a breast cancer cell line, showing that this gene plays a role in the development and/or progression of breast cancer. This gene was also expressed at fairly high levels in pancreas, and testis. Expression in pancreas demonstrates that this gene or gene product is involved in metabolic diseases such as diabetes and obesity.


Panel 1.1 Summary: Ag436b The expression of this gene was highest in testes and one breast cancer cell line. Expression of this gene is useful as a unique and specific marker for normal testis tissue. Therapeutic modulation of this gene or gene product through the use of protein, antibody, or small molecule therapeutics is useful as a therapeutic for breast cancer.


Panel 1.3D Summary: Ag436 The expression of this gene was highest in testis and one breast cancer cell line.


Panel 2D Summary: Ag436 The expression of this gene in panel 2D was restricted to one bladder cancer, one kidney cancer and one breast cancer. The expression detected in breast cancer was also in agreement with the results observed for panel 1.3D.


Thus, therapeutic modulation of this gene or its protein product is useful in the treatment breast, bladder or kidney cancer.


Panel 3D Summary: Ag436 Expression of this gene in panel 3D was restricted to one sample of a plasmacytoma cell line and two lung cancer cell lines. Thus, therapeutic modulation of this gene or gene product is useful in the treatment of plasmacytoma or lung cancer.


Panel 4D Summary: Ag436 This gene was expressed in the kidney and in the KU-812 basophil cell line. Basophils are found in the kidney and may give rise to the signal observed in this tissue (Maekawa, K. FEBS Lett 1994 January 10;337(2):200-6). Therefore, therapeutics targeting this gene or its protein product are useful for the detection of basophils in a variety of tissues.


U. CG52988-04 and CG52988-06 and CG52988-07: GAGE


Expression of full-length physical clones CG52988-04, CG52988-06, and CG52988-07 was assessed using the primer-probe sets Ag437 and Ag7222, described in Tables UA and UB. Results of the RTQ-PCR runs are shown in Tables UC and UD.

TABLE UAProbe Name Ag437PrimersSequencesLengthStart PositionSEQ ID NoForward5′-tcttcccagccagttggatct-3′212601345ProbeTET-5′-tcctcagtgggctcctggacaatca-3′-TAMRA252341346Reverse5′-cagttggtggttcctcttcttga-3′232051347









TABLE UB










Probe Name Ag7222











Primers
Sequences
Length
Start Position
SEQ ID No















Forward
5′-ttgcacctagtggggagatc-3′
20
173
1348






Probe
TET-5′-acctgccgttcaagggcctgac-3′-TAMRA
22
137
1349





Reverse
5′-cagagccagttcctgttgaa-3′
20
107
1350
















TABLE UC










General_screening_panel_v1.7










Tissue Name
A














Adipose
1.4



HUVEC
0.3



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.8



Melanoma (met) SK-MEL-5
0.0



Testis
98.6



Prostate ca. (bone met) PC-3
0.0



Prostate ca. DU145
0.3



Prostate pool
0.0



Uterus pool
0.0



Ovarian ca. OVCAR-3
0.3



Ovarian ca. (ascites) SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.2



Ovarian ca. OVCAR-5
1.5



Ovarian ca. IGROV-1
0.7



Ovarian ca. OVCAR-8
2.1



Ovary
0.9



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
6.4



Breast ca. BT-549
0.3



Breast ca. T47D
0.1



Breast pool
0.0



Trachea
2.6



Lung
1.6



Fetal Lung
2.5



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
100.0



Lung ca. SHP-77
0.7



Lung ca. NCI-H23
2.4



Lung ca. NCI-H460
1.2



Lung ca. HOP-62
2.0



Lung ca. NCI-H522
0.6



Lung ca. DMS-114
0.0



Liver
0.0



Fetal Liver
5.1



Kidney pool
1.1



Fetal Kidney
0.9



Renal ca. 786-0
0.5



Renal ca. A498
0.5



Renal ca. ACHN
0.6



Renal ca. UO-31
0.0



Renal ca. TK-10
0.3



Bladder
1.9



Gastric ca. (liver met.) NCI-N87
0.0



Stomach
0.0



Colon ca. SW-948
0.3



Colon ca. SW480
0.6



Colon ca. (SW480 met) SW620
2.4



Colon ca. HT29
0.0



Colon ca. HCT-116
2.1



Colon cancer tissue
0.0



Colon ca. SW1116
0.8



Colon ca. Colo-205
0.6



Colon ca. SW-48
0.0



Colon
0.9



Small Intestine
0.3



Fetal Heart
0.3



Heart
0.0



Lymph Node pool 1
0.0



Lymph Node pool 2
2.9



Fetal Skeletal Muscle
1.1



Skeletal Muscle pool
0.2



Skeletal Muscle
0.3



Spleen
1.6



Thymus
0.9



CNS cancer (glio/astro) SF-268
0.0



CNS cancer (glio/astro) T98G
0.0



CNS cancer (neuro; met) SK-N-AS
1.3



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.3



CNS cancer (glio) SNB-19
0.9



CNS cancer (glio) SF-295
6.8



Brain (Amygdala)
0.3



Brain (Cerebellum)
1.2



Brain (Fetal)
1.0



Brain (Hippocampus)
0.3



Cerebral Cortex pool
0.5



Brain (Substantia nigra)
0.0



Brain (Thalamus)
0.2



Brain (Whole)
2.1



Spinal Cord
0.4



Adrenal Gland
0.4



Pituitary Gland
0.2



Salivary Gland
0.0



Thyroid
0.9



Pancreatic ca. PANC-1
0.0



Pancreas pool
0.4









Column A - Rel. Exp. (%) Ag7222, Run 318040952














TABLE UD










Panel 4D










Tissue Name
A














Secondary Th1 act
21.9



Secondary Th2 act
23.5



Secondary Tr1 act
17.3



Secondary Th1 rest
13.6



Secondary Th2 rest
7.7



Secondary Tr1 rest
13.6



Primary Th1 act
33.2



Primary Th2 act
19.5



Primary Tr1 act
15.7



Primary Th1 rest
43.5



Primary Th2 rest
22.7



Primary Tr1 rest
19.9



CD45RA CD4 lymphocyte act
18.8



CD45RO CD4 lymphocyte act
48.6



CD8 lymphocyte act
69.3



Secondary CD8 lymphocyte rest
30.6



Secondary CD8 lymphocyte act
42.9



CD4 lymphocyte none
30.4



2ry Th1/Th2/Tr1_anti-CD95 CH11
12.7



LAK cells rest
9.6



LAK cells IL-2
20.4



LAK cells IL-2 + IL-12
0.8



LAK cells IL-2 + IFN gamma
42.3



LAK cells IL-2 + IL-18
18.2



LAK cells PMA/ionomycin
19.5



NK Cells IL-2 rest
17.2



Two Way MLR 3 day
26.2



Two Way MLR 5 day
11.3



Two Way MLR 7 day
5.5



PBMC rest
18.0



PBMC PWM
41.5



PBMC PHA-L
24.3



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
10.2



B lymphocytes PWM
81.2



B lymphocytes CD40L and IL-4
20.3



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
10.4



Dendritic cells LPS
6.3



Dendritic cells anti-CD40
0.0



Monocytes rest
8.7



Monocytes LPS
4.4



Macrophages rest
5.4



Macrophages LPS
11.3



HUVEC none
0.0



HUVEC starved
10.4



HUVEC IL-1beta
5.1



HUVEC IFN gamma
6.3



HUVEC TNF alpha + IFN gamma
14.9



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
4.8



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
9.5



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
31.0



Small airway epithelium none
12.2



Small airway epithelium TNFalpha + IL-1beta
12.3



Coronery artery SMC rest
7.0



Coronery artery SMC TNFalpha + IL-1beta
25.2



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
14.8



KU-812 (Basophil) rest
62.0



KU-812 (Basophil) PMA/ionomycin
100.0



CCD1106 (Keratinocytes) none
6.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
20.2



Liver cirrhosis
20.7



Lupus kidney
15.7



NCI-H292 none
26.1



NCI-H292 IL-4
12.2



NCI-H292 IL-9
6.1



NCI-H292 IL-13
19.6



NCI-H292 IFN gamma
15.8



HPAEC none
5.4



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
12.4



Lung fibroblast IL-4
17.6



Lung fibroblast IL-9
23.2



Lung fibroblast IL-13
14.5



Lung fibroblast IFN gamma
12.9



Dermal fibroblast CCD1070 rest
7.0



Dermal fibroblast CCD1070 TNF alpha
26.4



Dermal fibroblast CCD1070 IL-1 beta
2.3



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
11.0



IBD Colitis 2
12.2



IBD Crohn's
16.4



Colon
18.7



Lung
12.2



Thymus
12.3



Kidney
37.6









Column A - Rel. Exp. (%) Ag437, Run 145778415







General_screening13 panel_v1.7 Summary: Ag7222 Highest expression of this gene was seen in the testis and a lung cancer cell line sample (CTs=27). Thus, therapeutics targeting this gene or its protein product are useful in the treatment of male infertility or lung cancer.


Panel 4D Summary: Ag437 Highest expression was seen in the basophil cell line, KU-812 (CTs=33). Basophils release histamines and other biological modifiers in reponse to allergens and play an important role in the pathology of asthma and hypersensitivity reactions. Therefore, therapeutics designed against the protein encoded by this gene are useful for the reduction or inhibition of inflammation by blocking basophil function in these diseases. In addition, these cells are a reasonable model for the inflammatory cells that take part in various inflammatory lung and bowel diseases, such as asthma, Crohn's disease, and ulcerative colitis. Therefore, therapeutics that modulate the function of this gene or gene product are useful for the reduction or elimination of the symptoms of patients suffering from asthma, Crohn's disease, and ulcerative colitis.


V. CG5344903: Butyrophilin Like Receptor


Expression of gene CG53449-03 was assessed using the primer-probe sets Ag2030 and Ag8336, described in Tables VA and VB. Results of the RTQ-PCR runs are shown in Tables VC and VD.

TABLE VAProbe Name Ag2030SEQStartIDPrimersSequencesLengthPositionNoForward5′-ctgcgtttctgatctgaaaact-3′229971351ProbeTET-5′-acccatagaaaagctccccaggaggt-3′-2610221352TAMRAReverse5′-ccaccacactcttccttgtaaa-3′2210671353









TABLE VB










Probe Name Ag8336
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-cattgttggactgaagattttctt-3′
24
844
1354






Probe
TET-5′-ctccaaattccagtggaaaatccagg-3′-
26
868
1355



TAMRA





Reverse
5′-cctgtccgtgctttcttctc-3′
20
907
1356
















TABLE VC










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.0



Pancreas
3.3



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
0.0



Thyroid
3.8



Salivary gland
0.3



Pituitary gland
0.0



Brain (fetal)
0.0



Brain (whole)
0.0



Brain (amygdala)
0.3



Brain (cerebellum)
0.0



Brain (hippocampus)
0.0



Brain (substantia nigra)
0.0



Brain (thalamus)
0.3



Cerebral Cortex
0.0



Spinal cord
3.2



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.0



glioma U251
0.0



glioma SF-295
0.9



Heart (fetal)
0.0



Heart
0.0



Skeletal muscle (fetal)
0.0



Skeletal muscle
0.0



Bone marrow
30.6



Thymus
0.0



Spleen
3.0



Lymph node
4.3



Colorectal
73.7



Stomach
15.7



Small intestine
100.0



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
40.1



Colon ca. tissue(ODO3866)
1.9



Colon ca. HCC-2998
8.1



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
10.7



Trachea
1.6



Kidney
0.0



Kidney (fetal)
6.5



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
1.6



Liver ca. (hepatoblast) HepG2
0.0



Lung
27.9



Lung (fetal)
12.4



Lung ca. (small cell) LX-1
0.4



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
7.6



Lung ca. (non-sm. cell) A549
1.2



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
0.0



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
0.0



Breast ca. MDA-N
0.0



Ovary
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
1.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0



Uterus
0.8



Placenta
0.0



Prostate
0.0



Prostate ca.* (bone met)PC-3
0.0



Testis
15.8



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
1.8









Column A - Rel. Exp. (%) Ag2030, Run 165618974














TABLE VD










Panel 4.1D









Tissue Name
A
B












Secondary Th1 act
2.3
0.0


Secondary Th2 act
0.8
0.0


Secondary Tr1 act
0.0
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
0.0


Secondary Tr1 rest
0.0
0.0


Primary Th1 act
0.0
0.0


Primary Th2 act
0.0
0.0


Primary Tr1 act
1.7
0.0


Primary Th1 rest
0.0
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
0.0


CD45RO CD4 lymphocyte act
2.3
3.6


CD8 lymphocyte act
1.2
0.0


Secondary CD8 lymphocyte rest
0.0
0.0


Secondary CD8 lymphocyte act
1.4
0.0


CD4 lymphocyte none
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0


LAK cells rest
0.0
0.0


LAK cells IL-2
0.0
0.0


LAK cells IL-2 + IL-12
0.0
0.0


LAK cells IL-2 + IFN gamma
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0


NK Cells IL-2 rest
0.0
0.0


Two Way MLR 3 day
0.0
0.0


Two Way MLR 5 day
0.0
0.0


Two Way MLR 7 day
0.0
0.0


PBMC rest
0.0
0.0


PBMC PWM
1.1
0.0


PBMC PHA-L
0.0
0.0


Ramos (B cell) none
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0


B lymphocytes PWM
4.7
0.0


B lymphocytes CD40L and IL-4
0.0
0.0


EOL-1 dbcAMP
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0


Dendritic cells none
0.0
0.0


Dendritic cells LPS
0.0
0.0


Dendritic cells anti-CD40
0.0
0.0


Monocytes rest
0.0
0.0


Monocytes LPS
0.0
0.0


Macrophages rest
0.0
0.0


Macrophages LPS
0.0
0.0


HUVEC none
0.0
0.0


HUVEC starved
0.0
0.0


HUVEC IL-1beta
0.0
0.0


HUVEC IFN gamma
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0


HUVEC IL-11
0.0
0.0


Lung Microvascular EC none
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
0.0
0.0


Microvascular Dermal EC none
0.0
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.0


Bronchial epithelium TNFalpha + IL1beta
0.0
0.0


Small airway epithelium none
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
0.0
0.0


Coronery artery SMC rest
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
0.0
0.0


Astrocytes TNFalpha + IL-1beta
0.0
0.0


KU-812 (Basophil) rest
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0


CCD1106 (Keratinocytes) none
0.0
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0
0.0


Liver cirrhosis
12.9
1.3


NCI-H292 none
0.0
3.7


NCI-H292 IL-4
0.0
0.0


NCI-H292 IL-9
0.0
0.0


NCI-H292 IL-13
0.0
0.0


NCI-H292 IFN gamma
0.0
0.0


HPAEC none
0.0
0.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast none
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast IL-4
0.0
0.0


Lung fibroblast IL-9
0.0
0.0


Lung fibroblast IL-13
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.7
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.0


Dermal fibroblast IFN gamma
0.0
0.0


Dermal fibroblast IL-4
0.0
0.0


Dermal Fibroblasts rest
0.0
0.0


Neutrophils TNFa + LPS
23.2
11.6


Neutrophils rest
84.1
100.0


Colon
100.0
5.7


Lung
2.4
0.0


Thymus
0.0
0.0


Kidney
0.8
0.0







Column A - Rel. Exp. (%) Ag2030, Run 306413257





Column B - Rel. Exp. (%) Ag8336, Run 343540037







Panel 1.3D Summary: Ag2030 This gene was most prominently expressed in gastrointestinal tissues. Expression of this gene was highest in colon and small intestine (CT=31) and was also significantly expressed in stomach tissue. Therapeutic modulation of this gene or its protein product is useful as a marker of gastrointestinal tissue, and specifically of small intestine, stomach or colorectal tissue.


Panel 4.1D Summary: Ag2030/Ag8336 Expression of this gene was limited to colon and neutrophils (CTs=30-34). Expression of this gene was down-regulated in activated neutrophils. This expression profile demonstrated that the encoded protein reduces activation of these inflammatory cells and is useful as a protein therapeutic to eliminate the symptoms in patients with Crohn's disease, ulcerative colitis, multiple sclerosis, chronic obstructive pulmonary disease, asthma, emphysema, rheumatoid arthritis, lupus erythematosus, or psoriasis. In addition, small molecule or antibodies targeting this gene or gene product are effective in increasing the immune response in patients with AIDS or other immunodeficiencies.


W. CG53449-04: B7/Butyrophilin Like Receptor


Expression of gene CG53449-04 was assessed using the primer-probe sets Ag2030, Ag6640 and Ag7598, described in Tables WA, WB and WC. Results of the RTQ-PCR runs are shown in Tables WD, WE, WF, WG, WH and WI.

TABLE WAProbe Name Ag2030SEQStartIDPrimersSequencesLengthPositionNoForward5′-ctgcgtttctgatctgaaaact-3′2211851357ProbeTET-5′-acccatagaaaagctccccaggaggt-3′-2612101358TAMRAReverse5′-ccaccacactcttccttgtaaa-3′2212551359









TABLE WB










Probe Name Ag6640
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gtgtaagcgagagagagaagcat-3′
23
938
1360






Probe
TET-5′-atctctgatcctgtccctgctggaac-3′-
26
981
1361



TAMRA





Reverse
5′-tagaagaagagaagcagctggat-3′
23
1015
1362
















TABLE WC










Probe Name Ag7598
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-tgctcccacatccagct-3′
17
1006
1363






Probe
TET-5′-ttcttctagtcctagcctccagggg-3′-
25
1030
1364



TAMRA





Reverse
5′-gctttcttctccagtccagttc-3′
22
1084
1365
















TABLE WD










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
13.5



110980 COPD-F
25.5



110968 COPD-M
15.1



110977 COPD-M
42.0



110989 Emphysema-F
6.3



110992 Emphysema-F
0.0



110993 Emphysema-F
6.7



110994 Emphysema-F
2.0



110995 Emphysema-F
1.0



110996 Emphysema-F
0.0



110997 Asthma-M
1.6



111001 Asthma-F
6.0



111002 Asthma-F
4.0



111003 Atopic Asthma-F
3.5



111004 Atopic Asthma-F
3.4



111005 Atopic Asthma-F
4.5



111006 Atopic Asthma-F
0.0



111417 Allergy-M
4.5



112347 Allergy-M
0.4



112349 Normal Lung-F
0.4



112357 Normal Lung-F
8.9



112354 Normal Lung-M
4.8



112374 Crohns-F
4.2



112389 Match Control Crohns-F
7.2



112375 Crohns-F
1.9



112732 Match Control Crohns-F
3.9



112725 Crohns-M
3.5



112387 Match Control Crohns-M
0.3



112378 Crohns-M
1.2



112390 Match Control Crohns-M
24.5



112726 Crohns-M
2.0



112731 Match Control Crohns-M
4.5



112380 Ulcer Col-F
7.2



112734 Match Control Ulcer Col-F
15.7



112384 Ulcer Col-F
13.1



112737 Match Control Ulcer Col-F
3.1



112386 Ulcer Col-F
5.6



112738 Match Control Ulcer Col-F
19.9



112381 Ulcer Col-M
0.3



112735 Match Control Ulcer Col-M
0.0



112382 Ulcer Col-M
18.7



112394 Match Control Ulcer Col-M
3.4



112383 Ulcer Col-M
2.6



112736 Match Control Ulcer Col-M
6.2



112423 Psoriasis-F
12.3



112427 Match Control Psoriasis-F
25.2



112418 Psoriasis-M
26.8



112723 Match Control Psoriasis-M
2.9



112419 Psoriasis-M
18.3



112424 Match Control Psoriasis-M
11.7



112420 Psoriasis-M
32.8



112425 Match Control Psoriasis-M
14.2



104689 (MF) OA Bone-Backus
24.5



104690 (MF) Adj “Normal” Bone-Backus
38.4



104691 (MF) OA Synovium-Backus
6.4



104692 (BA) OA Cartilage-Backus
0.0



104694 (BA) OA Bone-Backus
6.0



104695 (BA) Adj “Normal” Bone-Backus
37.4



104696 (BA) OA Synovium-Backus
3.4



104700 (SS) OA Bone-Backus
88.3



104701 (SS) Adj “Normal” Bone-Backus
30.4



104702 (SS) OA Synovium-Backus
28.3



117093 OA Cartilage Rep7
15.2



112672 OA Bone5
13.4



112673 OA Synovium5
7.5



112674 OA Synovial Fluid cells5
6.0



117100 OA Cartilage Rep14
4.5



112756 OA Bone9
14.2



112757 OA Synovium9
24.1



112758 OA Synovial Fluid Cells9
37.9



117125 RA Cartilage Rep2
10.6



113492 Bone2 RA
100.0



113493 Synovium2 RA
47.0



113494 Syn Fluid Cells RA
68.3



113499 Cartilage4 RA
67.8



113500 Bone4 RA
81.2



113501 Synovium4 RA
64.2



113502 Syn Fluid Cells4 RA
51.1



113495 Cartilage3 RA
68.8



113496 Bone3 RA
66.9



113497 Synovium3 RA
52.5



113498 Syn Fluid Cells3 RA
85.9



117106 Normal Cartilage Rep20
1.1



113663 Bone3 Normal
0.0



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
0.0



117107 Normal Cartilage Rep22
7.9



113667 Bone4 Normal
5.1



113668 Synovium4 Normal
3.4



113669 Syn Fluid Cells4 Normal
3.7









Column A - Rel. Exp. (%) Ag6640, Run 296559279














TABLE WE










General_screening_panel_v1.6










Tissue Name
A














Adipose
11.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
16.5



Prostate ca.* (bone met) PC-3
0.1



Prostate Pool
1.4



Placenta
0.9



Uterus Pool
0.3



Ovarian ca. OVCAR-3
0.2



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
3.8



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.0



Ovary
2.9



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
2.5



Trachea
1.2



Lung
0.1



Fetal Lung
17.8



Lung ca. NCI-N417
0.0



Lung ca. LX-1
14.9



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.2



Lung ca. A549
1.3



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
1.1



Lung ca. NCI-H460
1.1



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
1.3



Liver ca. HepG2
0.0



Kidney Pool
5.6



Fetal Kidney
10.3



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
13.0



Gastric ca. (liver met.) NCI-N87
0.3



Gastric ca. KATO III
0.6



Colon ca. SW-948
1.4



Colon ca. SW480
0.8



Colon ca.* (SW480 met) SW620
0.1



Colon ca. HT29
0.4



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
100.0



Colon cancer tissue
9.3



Colon ca. SW1116
0.0



Colon ca. Colo-205
7.0



Colon ca. SW-48
26.2



Colon Pool
1.3



Small Intestine Pool
2.3



Stomach Pool
1.6



Bone Marrow Pool
0.6



Fetal Heart
2.1



Heart Pool
2.1



Lymph Node Pool
2.1



Fetal Skeletal Muscle
4.5



Skeletal Muscle Pool
0.0



Spleen Pool
8.2



Thymus Pool
1.7



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
8.4



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.3



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
0.4



Brain (fetal)
0.3



Brain (Hippocampus) Pool
0.8



Cerebral Cortex Pool
3.8



Brain (Substantia nigra) Pool
0.0



Brain (Thalamus) Pool
0.0



Brain (whole)
1.0



Spinal Cord Pool
0.4



Adrenal Gland
1.6



Pituitary gland Pool
0.0



Salivary Gland
0.7



Thyroid (female)
0.3



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
6.0









Column A - Rel. Exp. (%) Ag6640, Run 277241469














TABLE WF










PGI1.0










Tissue Name
A














162191_Normal Lung 1 (IBS)
4.6



162570_Normal Lung 4 (Aastrand)
2.0



160468_MD lung
2.9



156629_MD Lung 13
1.6



162571 _Normal Lung 3 (Aastrand)
0.7



162186_Fibrosis Lung 1 (Genomic
26.4



Collaborative)



162187_Fibrosis Lung 2 (Genomic
31.6



Collaborative)



151281_Fibrosis lung 11(Ardais)
45.1



162190_Asthma Lung 4 (Genomic
45.7



Collaborative)



160467_Asthma Lung 13 (MD)
2.1



137027_Emphysema Lung 1 (Ardais)
6.0



137028_Emphysema Lung 2 (Ardais)
5.7



137040_Emphysema Lung 3 (Ardais)
7.0



137041_Emphysema Lung 4 (Ardais)
14.1



137043_Emphysema Lung 5 (Ardais)
7.5



142817_Emphysema Lung 6 (Ardais)
20.4



142818_Emphysema Lung 7 (Ardais)
19.3



142819_Emphysema Lung 8 (Ardais)
10.4



142820_Emphysema Lung 9 (Ardais)
1.8



142821_Emphysema Lung 10 (Ardais)
12.6



162185_Emphysema Lung 12 (Ardais)
13.9



162184_Emphysema Lung 13 (Ardais)
5.3



162183_Emphysema Lung 14 (Ardais)
28.5



162188_Emphysema Lung 15 (Genomic
20.2



Collaborative)



162177_NAT UC Colon 1(Ardais)
100.0



162176_UC Colon 1(Ardais)
59.9



162179_NAT UC Colon 2(Ardais)
65.5



162178_UC Colon 2(Ardais)
8.1



162181_NAT UC Colon 3(Ardais)
63.7



162180_UC Colon 3(Ardais)
5.2



162182_NAT UC Colon 4 (Ardais)
100.0



137042_UC Colon 1108
4.2



137029_UC Colon 8215
8.1



137031_UC Colon 8217
5.6



137036_UC Colon 1137
22.2



137038_UC Colon 1491
13.4



137039_UC Colon 1546
27.0



162594_NAT Crohn's 47751 (NDRI)
3.7



162593_Crohn's 47751 (NDRI)
0.5









Column A - Rel. Exp. (%) Ag6640, Run 376378448














TABLE WG










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.0



Pancreas
3.3



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
0.0



Thyroid
3.8



Salivary gland
0.3



Pituitary gland
0.0



Brain (fetal)
0.0



Brain (whole)
0.0



Brain (amygdala)
0.3



Brain (cerebellum)
0.0



Brain (hippocampus)
0.0



Brain (substantia nigra)
0.0



Brain (thalamus)
0.3



Cerebral Cortex
0.0



Spinal cord
3.2



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.0



glioma U251
0.0



glioma SF-295
0.9



Heart (fetal)
0.0



Heart
0.0



Skeletal muscle (fetal)
0.0



Skeletal muscle
0.0



Bone marrow
30.6



Thymus
0.0



Spleen
3.0



Lymph node
4.3



Colorectal
73.7



Stomach
15.7



Small intestine
100.0



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
40.1



Colon ca. tissue(ODO3866)
1.9



Colon ca. HCC-2998
8.1



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
10.7



Trachea
1.6



Kidney
0.0



Kidney (fetal)
6.5



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
1.6



Liver ca. (hepatoblast) HepG2
0.0



Lung
27.9



Lung (fetal)
12.4



Lung ca. (small cell) LX-1
0.4



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
7.6



Lung ca. (non-sm. cell) A549
1.2



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
0.0



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
0.0



Breast ca. MDA-N
0.0



Ovary
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
1.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0



Uterus
0.8



Placenta
0.0



Prostate
0.0



Prostate ca.* (bone met)PC-3
0.0



Testis
15.8



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
1.8









Column A - Rel. Exp. (%) Ag2030, Run 165618974














TABLE WH










Panel 4.1D











Tissue Name
A
B
C
D














Secondary Th1 act
2.3
0.0
0.0
0.0


Secondary Th2 act
0.8
0.0
0.0
0.0


Secondary Tr1 act
0.0
0.0
0.0
0.0


Secondary Th1 rest
0.0
0.0
0.0
0.0


Secondary Th2 rest
0.0
0.0
0.0
0.0


Secondary Tr1 rest
0.0
0.0
0.0
0.0


Primary Th1 act
0.0
0.0
0.0
0.0


Primary Th2 act
0.0
0.0
0.0
0.0


Primary Tr1 act
1.7
0.0
0.0
1.6


Primary Th1 rest
0.0
0.0
0.0
0.0


Primary Th2 rest
0.0
0.0
0.0
0.0


Primary Tr1 rest
0.0
0.0
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
0.9
0.0
0.0


CD45RO CD4 lymphocyte act
2.3
3.0
4.0
9.5


CD8 lymphocyte act
1.2
0.0
0.7
0.0


Secondary CD8 lymphocyte rest
0.0
0.0
0.0
0.0


Secondary CD8 lymphocyte act
1.4
0.7
0.0
0.0


CD4 lymphocyte none
0.0
0.0
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0
0.9
0.0


LAK cells rest
0.0
0.0
1.5
0.0


LAK cells IL-2
0.0
0.0
0.0
0.0


LAK cells IL-2 + IL-12
0.0
0.0
0.0
0.0


LAK cells IL-2 + IFN gamma
0.0
0.0
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.0
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0
0.0
0.0


NK Cells IL-2 rest
0.0
0.0
0.0
0.0


Two Way MLR 3 day
0.0
0.0
0.0
0.0


Two Way MLR 5 day
0.0
0.0
1.1
0.0


Two Way MLR 7 day
0.0
1.5
0.0
0.0


PBMC rest
0.0
0.0
0.0
0.0


PBMC PWM
1.1
0.0
0.5
0.0


PBMC PHA-L
0.0
0.0
0.5
0.0


Ramos (B cell) none
0.0
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0
0.0


B lymphocytes PWM
4.7
2.8
2.7
1.5


B lymphocytes CD40L and IL-4
0.0
2.3
0.0
0.0


EOL-1 dbcAMP
0.0
0.0
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0
0.0


Dendritic cells none
0.0
0.0
0.0
0.0


Dendritic cells LPS
0.0
0.0
0.0
0.0


Dendritic cells anti-CD40
0.0
0.0
0.0
0.0


Monocytes rest
0.0
0.0
0.3
0.0


Monocytes LPS
0.0
0.0
0.0
0.0


Macrophages rest
0.0
0.0
0.0
0.0


Macrophages LPS
0.0
0.0
0.0
0.0


HUVEC none
0.0
0.0
0.0
0.0


HUVEC starved
0.0
0.0
0.0
0.0


HUVEC IL-1 beta
0.0
0.0
0.0
0.0


HUVEC IFN gamma
0.0
0.0
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0
0.0
0.0


HUVEC IL-11
0.0
0.0
0.0
0.0


Lung Microvascular EC none
0.0
0.0
0.0
1.7


Lung Microvascular EC
0.0
0.0
0.0
0.0


TNFalpha + IL-1beta


Microvascular Dermal EC none
0.0
0.0
1.1
0.0


Microsvasular Dermal EC
0.0
0.0
0.0
0.0


TNFalpha + IL-1beta


Bronchial epithelium
0.0
0.0
0.0
0.0


TNFalpha + IL1beta


Small airway epithelium none
0.0
0.0
0.0
0.0


Small airway epithelium
0.0
0.0
0.0
0.0


TNFalpha + IL-1beta


Coronery artery SMC rest
0.0
0.0
0.0
0.0


Coronery artery SMC
0.0
0.0
0.0
0.0


TNFalpha + IL-1beta


Astrocytes rest
0.0
0.0
0.0
0.0


Astrocytes TNFalpha + IL-1beta
0.0
0.0
0.0
0.0


KU-812 (Basophil) rest
0.0
0.0
0.9
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0
0.0
0.0


CCD1106 (Keratinocytes) none
0.0
0.0
0.0
0.0


CCD1106 (Keratinocytes)
0.0
0.0
0.0
0.0


TNFalpha + IL-1beta


Liver cirrhosis
12.9
2.8
6.0
0.0


NCI-H292 none
0.0
2.4
0.0
0.0


NCI-H292 IL-4
0.0
2.4
0.0
1.7


NCI-H292 IL-9
0.0
1.1
3.7
1.3


NCI-H292 IL-13
0.0
0.0
1.6
1.6


NCI-H292 IFN gamma
0.0
0.0
0.0
0.0


HPAEC none
0.0
0.0
0.0
0.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0
0.0
0.0


Lung fibroblast none
0.0
0.0
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0
0.0
0.0


Lung fibroblast IL-4
0.0
0.0
0.0
0.0


Lung fibroblast IL-9
0.0
0.0
0.0
0.0


Lung fibroblast IL-13
0.0
0.0
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.7
0.0
0.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.0
0.0
0.0


Dermal fibroblast IFN gamma
0.0
0.0
1.1
0.0


Dermal fibroblast IL-4
0.0
0.0
0.0
0.0


Dermal Fibroblasts rest
0.0
0.0
0.0
0.0


Neutrophils TNFa + LPS
23.2
31.0
20.6
38.4


Neutrophils rest
84.1
100.0
100.0
100.0


Colon
100.0
23.8
25.5
40.3


Lung
2.4
0.0
0.7
0.0


Thymus
0.0
0.8
0.0
0.0


Kidney
0.8
3.0
1.3
0.0







Column A - Rel. Exp. (%) Ag2030, Run 306413257





Column B - Rel. Exp. (%) Ag6640, Run 274991372





Column C - Rel. Exp. (%) Ag6640, Run 296907633





Column D - Rel. Exp. (%) Ag7598, Run 310594484














TABLE WI










Panel 4D










Tissue Name
A














Secondary Th1 act
0.1



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.1



Primary Tr1 act
0.1



Primary Th1 rest
0.1



Primary Th2 rest
0.1



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.1



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.2



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.1



LAK cells rest
0.1



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.1



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.3



PBMC PHA-L
0.1



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.1



B lymphocytes PWM
2.5



B lymphocytes CD40L and IL-4
0.1



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1 beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.1



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.1



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
0.6



Lupus kidney
0.0



NCI-H292 none
0.1



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.1



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



IBD Colitis 2
0.0



IBD Crohn's
5.3



Colon
100.0



Lung
0.5



Thymus
0.5



Kidney
0.0









Column A - Rel. Exp. (%) Ag2030, Run 161379401







AI_comprehensive panel_v1.0 Summary: Ag6640 Highest expression of this gene was detected in rheumatoid arthritis bone (CT=31). This gene was expressed at higher levels in rheumatoid arthritis (RA) tissues and osteoarthritis (OA) bone. In addition, significant expression of this gene was also seen in COPD lung, and psoriasis samples. This gene encodes a homolog of butyrophilin like receptor and is a member of the B7 family. B7 family members are costimulatory molecules, which are necessary for optimal activation of T lymphocytes after T cell receptor (TCR) engagement. The most extensively studied costimulatory molecules are B7-1 (CD80) and B7-2 (CD86). Interaction of CD28 on T cells with either B7-1 or B7-2 augments T cell activation and promotes T cell survival (Chambers, C. A. & Allison, J. P., 1997, Curr. Opin. Immunol. 9, 396-404). In contrast, binding of B7-1 or B7-2 with cytotoxic T lymphocyte antigen 4 (CTLA-4), a homolog of CD28, inhibits the T cell response by delivering a putative negative signal. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drug targeting the gene or gene product is effective in the treatment of psoriases, osteoarthritis and rheumatoid arthritis.


General_screening13 panel_v1.6 Summary: Significant expression of this gene was seen in colon cancer cell lines, a lung cancer and a brain cancer cell lines. Therefore, expression of this gene is useful as a diagnostic marker and therapeutic modulation of this gene, expressed protein, and/or use of antibodies or small molecule drug targeting this gene or gene product is useful in the treatment of colon, lung and brain cancers.


Low expression of this gene was also seen in metabolic tissues including adipose, pancrease, and fetal skeletal muscle. Therefore, modulation of this gene or gene product is useful in the treatment of metabolic disorders such as diabetes and obesity.


Low expression of this gene was also seen fetal lung. Expression of this gene was higher in fetal (CTs=32-34) relative to adult lung and skeletal muscle (CTs=40). The relative overexpression of this gene in fetal tissue shows that the protein product enhances lung and skeletal muscle growth or development in the fetus and also acts in a regenerative capacity in the adult. Therapeutic modulation of this gene or the protein encoded by this gene is useful in the treatment of lung and skeletal muscle related diseases.


PGI1.0 Summary: Ag6640 Highest expression of this gene was detected in normal adjacent colon tissue from a ulcerative colitis patient (CT=26.4). Significant expression of this gene was also detected in normal adjacent and ulcerative colitis tissue samples. Expression of this gene was higher in normal relative to diseased tissue. Modulation of this gene and/or its expressed protein is useful in the treatment of ulcerative colitis.


In addition, high to moderate expression of this gene was also detected in fibrosis, asthma and emphyzema lung. Therapeutic modulation of this gene, expressed protein is useful in the treatment of fibrosis, asthma and emphysema.


Panel 1.3D Summary: Ag2030 This gene was most prominently expressed in gastrointestinal tissues. Expression of this gene was highest in colon and small intestine (CT=31) and was also significantly expressed in stomach tissue. Expression of this gene is useful as a marker of gastrointestinal tissue, and specifically of small intestine, stomach or colorectal tissue.


Panel 4.1D Summary: Ag2030/Ag6640/Ag7598 Expression of this gene was limited to colon and neutrophils (CTs=30-34). Expression of this gene was down-regulated in activated neutrophils. This expression profile demonstrates that the encoded protein reduces activation of these inflammatory cells and is useful as a protein therapeutic to eliminate the symptoms in patients with Crohn's disease, ulcerative colitis, multiple sclerosis, chronic obstructive pulmonary disease, asthma, emphysema, rheumatoid arthritis, lupus erythematosus, or psoriasis. In addition, small molecule or antibodies targeting this gene or gene product are effective in increasing the immune response in patients with AIDS or other immunodeficiencies.


Panel 4D Summary: Ag2030 The gene expression on this panel was limited essentially to normal colon. These results were consistent with what is observed in Panel 1.3D. This gene was not expressed or was expressed only at low levels in IBD colitis and Crohn's disease. Therefore, this gene is useful as a marker to distinguish normal colon from diseased colon. This gene encodes a protein with homology to the butyrophilin-like receptor. The butyrophilin-like membrane proteins are similar to the B7 family of co-stimulatory factors. B7 proteins are expressed on many cell types and function in the process of antigen presentation to T lymphocytes in the stimulation of the immune response. Recently identified B7 family members have included inhibitory proteins that reduced the activation of T lymphocytes upon cell-cell interaction. This gene or gene product is useful for the modulation of the functions associated with antigen presentation to T cells. Replacement of this gene product by gene therapy or use of the isolated extracellular domain of the gene protein is of use as a therapeutic in the treatment of IBD colitis and Crohn's disease.


X. CG53908-01: Netrin Receptor Unc5h1


Expression of full-length physical clone CG53908-01 was assessed using the primer-probe sets Ag1395 and Ag8392, described in Tables XA and XB. Results of the RTQ-PCR runs are shown in Tables XC, XD, XE, XF, XG, XH, XL XJ, and XK.

TABLE XAProbe Name Ag1395SEQStartIDPrimersSequencesLengthPositionNoForward5′-ctgcacttcaaggacagttacc-3′2221841366ProbeTET-5′-ctatccatccacgatgtgcccagct-3′-2522171367TAMRAReverse5′-tgacaaggagcttactcttcca-3′2222471368









TABLE XB










Probe Name Ag8392
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ccctctatgtgggcctcat-3′
19
1168
1369






Probe
TET-5′-tgcttgtcctcatcctcgtttattgc-3′-
26
1216
1370



TAMRA





Reverse
5′-tccagcccctccttcttc-3′
18
1244
1371
















TABLE XC










Ardais Breast1.0











Tissue Name
A
B















97739_Breast cancer (CHTN20676)
0.6
0.2



105689_Breast cancer 2B
0.0
0.1



111297_Breast cancer metastasis (9369)*
23.0
17.8



145848_Breast cancer (9B6)
8.7
6.0



145859_Breast cancer (9EC)
1.0
0.6



153632_Breast cancer (D39)
1.0
0.2



153643_Breast cancer (D44)
6.0
2.6



164672_Breast cancer (7464)
9.1
4.5



164681_Breast cancer (5787)
6.8
3.3



97748_Breast cancer (CHTN20931)
9.3
0.1



105690_Breast NAT 2A
1.7
0.6



111302_Breast NAT (6314)
1.8
1.2



145850_Breast cancer (9C7)
2.8
0.8



149844_Breast cancer (24178)
3.3
2.6



153633_Breast cancer (D3A)
72.7
34.4



153644_Breast cancer (D45)
1.2
0.8



164673_Breast cancer (8452)
2.9
2.6



164682_Breast cancer (6342)*
2.1
1.5



97751_Breast cancer (CHTN21053)
3.5
3.2



105694_Breast NAT
15.8
10.6



116417_Breast cancer (3367)*
1.8
1.5



145852_Breast cancer (A1A)
1.0
0.4



151097_Breast cancer (CHTN24298)
0.9
0.3



153634_Breast cancer (D3B)
1.0
0.5



155797_Breast cancer (EA6)
81.2
67.8



164674_Breast cancer (8811)
2.8
2.4



164683_Breast cancer (6470)
100.0
100.0



97763_Breast cancer (OD06083)
3.7
2.4



108830_Breast cancer metastasis
1.1
0.4



(OD06855)*



116418_Breast cancer (3378)*
2.4
1.7



145853_Breast cancer (9F3)
1.6
0.7



153432_Breast cancer (CHTN24652)
15.6
6.2



153635_Breast cancer (D3C)
0.6
0.4



164667_Breast cancer (5785)
1.6
12.2



164676_Breast cancer (5070)
1.5
0.6



164684_Breast cancer (6509)
8.8
3.5



97764_Breast cancer node
4.4
4.8



metastasis (OD06083)



108847_Breast cancer
2.8
1.2



116421_Breast cancer (6314)
0.1
0.0



145854_Breast cancer (9B8)
1.2
0.4



153627_Breast cancer (D34)
5.8
3.6



153636_Breast cancer (D3D)
7.8
4.9



164668_Breast cancer (6314)
0.5
0.2



164677_Breast cancer (5272)
21.6
17.3



164685_Breast cancer (0170)
80.1
50.7



98857_Breast cancer (OD06397-12)
2.3
1.2



111288_Breast NAT (3367)
14.6
6.2



116424_Breast cancer 4B*
0.2
0.2



153628_Breast cancer (D35)
18.9
11.7



153637_Breast cancer (D3E)
1.3
0.9



164669_Breast cancer (6992)
16.0
9.6



164678_Breast cancer (5297)
50.0
42.6



164686_Breast cancer (0732)
0.8
0.5



105687_Breast cancer 1B
0.1
0.1



111289_Breast cancer 3B*
8.2
5.7



116425_Breast NAT 4A
0.3
0.3



145857_Breast cancer (9F0)
8.8
8.0



153630_Breast cancer (D37)
2.0
2.0



153638_Breast cancer (D3F)
11.0
10.0



164670_Breast cancer (7078)
1.7
2.7



164679_Breast cancer (5486)
15.8
13.9



164687_Breast cancer (5881)
11.2
4.0



105688_Breast NAT 1A
0.2
0.1



111290_Breast NAT 3A*
4.2
6.8



145846_Breast cancer (9B7)
7.1
9.6



145858_Breast cancer (9B4)
0.9
1.4



153631_Breast cancer (D38)
0.3
0.2



153639_Breast cancer (D40)
66.0
29.3



164671_Breast cancer (7082)
62.4
50.0



164680_Breast cancer (5705)
1.7
1.3



164688_Breast cancer (7222)
1.5
0.5









Column A - Rel. Exp. (%) Ag1395, Run 399018961






Column B - Rel. Exp. (%) Ag8392, Run 398657263














TABLE XD










Ardais Panel 1.1











Tissue Name
A
B















Lung adenocarcinoma SI A
15.6
8.7



Lung adenocarcinoma SI B
22.2
75.3



Lung adenocarcinoma SI B NAT
100.0
70.7



Lung adenocarcinoma SI C
1.6
3.4



Lung adenocarcinoma SI C NAT
98.6
100.0



Lung adenocarcinoma SII A
11.7
14.5



Lung adenocarcinoma SII A NAT
58.6
76.3



Lung adenocarcinoma SII C NAT
62.4
55.5



Lung adenocarcinoma SIII A
41.5
42.9



Lung adenocarcinoma SIII B
32.1
33.7



Lung adenocarcinoma SIII C
16.8
25.2



Lung SCC SI A
27.9
74.7



Lung SCC SI B NAT
77.4
33.4



Lung SCC SI C
23.3
31.0



Lung SCC SI C NAT
71.2
82.9



Lung SCC SI D
40.6
62.4



Lung SCC SI D NAT
32.5
59.9



Lung SCC SII A
15.4
60.3



Lung SCC SII B
7.0
11.7



Lung SCC SIII A
11.5
11.5



Lung SCC SIII A NAT
29.1
13.4









Column A - Rel. Exp. (%) Ag1395, Run 332737494






Column B - Rel. Exp. (%) Ag8392, Run 369781003














TABLE XE










Ardais Prostate 1.0











Tissue Name
A
B















145904_Prostate cancer(9E2)
100.0
100.0



149776_Prostate cancer(ADS)
17.4
20.0



151135_Prostate NAT(B87)
0.4
0.2



151143_Prostate NAT(B8A)
0.3
0.2



153653_Prostate cancer(D4E)
0.7
0.7



153661_Prostate cancer(D56)
1.2
0.7



153669_Prostate NAT(D5E)
1.2
0.6



153677_Prostate NAT(D66)
0.2
0.1



153685_Prostate NAT(D6E)
0.2
0.1



145905_Prostate NAT(A0C)
1.0
0.7



151128_Prostate cancer(B8C)
0.6
0.9



151136_Prostate cancer(B8B)
3.3
2.1



151144_Prostate cancer(B8F)
0.4
0.2



153654_Prostate cancer(D4F)
4.5
3.8



153662_Prostate cancer(D57)
0.2
0.0



153670_Prostate NAT(D5F)
1.1
0.6



153678_Prostate NAT(D67)
0.3
0.1



153686_Prostate NAT(D6F)
0.2
0.2



145906_Prostate NAT(A09)
0.4
0.3



151129_Prostate NAT(B93)
0.7
0.6



151137_Prostate NAT(B86)
0.3
0.4



151145_Prostate NAT(B91)
0.4
0.3



153655_Prostate cancer(D50)
0.7
0.4



153663_Prostate cancer(D58)
0.1
0.0



153671_Prostate NAT(D60)
0.2
0.6



153679_Prostate NAT(D68)
0.2
0.2



153687_Prostate NAT(D70)
0.4
0.3



145907_Prostate cancer(A0A)
0.4
0.3



151130_Prostate cancer(B90)
1.4
1.1



151138_Prostate cancer(B8D)
11.6
11.3



153648_Prostate cancer(D49)
2.1
1.8



153656_Prostate cancer(D51)
0.4
0.1



153664_Prostate cancer(D59)
1.2
0.9



153672_Prostate NAT(D61)
0.3
0.7



153680_Prostate NAT(D69)
0.3
0.3



155799_Prostate cancer(EA8)
2.6
3.8



145909_Prostate cancer(9E7)
3.1
4.4



151131_Prostate NAT(B85)
1.2
0.6



151139_Prostate NAT(B8E)
0.4
0.3



153649_Prostate cancer(D4A)
0.7
0.4



153657_Prostate cancer(D52)
0.6
0.2



153665_Prostate cancer(D5A)
34.6
25.7



153673_Prostate NAT(D62)
0.6
0.3



153681_Prostate NAT(D6A)
0.2
0.1



145910_Prostate NAT(9C3)
1.0
0.4



151132_Prostate cancer(B88)
12.9
7.4



151140_Prostate cancer(B95)
0.8
0.4



153650_Prostate cancer(D4B)
3.7
3.1



153658_Prostate cancer(D53)
1.2
0.4



153666_Prostate cancer(D5B)
15.7
14.3



153674_Prostate NAT(D63)
1.1
0.3



153682_Prostate NAT(D6B)
0.9
0.6



149773_Prostate NAT(AD8)
0.0
0.0



151133_Prostate NAT(B94)
0.5
0.4



151141_Prostate NAT(B96)
1.1
0.8



153651_Prostate cancer(D4C)
11.1
9.7



153659_Prostate cancer(D54)
3.5
3.0



153667_Prostate cancer(D5C)
0.6
0.3



153675_Prostate NAT(D64)
1.2
0.4



153683_Prostate NAT(D6C)
0.8
0.3



149774_Prostate cancer(AD7)
0.9
1.1



151134_Prostate cancer(B92)
0.4
0.2



151142_Prostate cancer(B89)
40.9
40.9



153652_Prostate cancer(D4D)
0.6
0.3



153660_Prostate cancer(D55)
12.8
11.5



153668_Prostate NAT(D5D)
0.4
0.3



153676_Prostate NAT(D65)
0.7
0.4



153684_Prostate NAT(D6D)
0.3
0.2









Column A - Rel. Exp. (%) Ag1395, Run 379587273






Column B - Rel. Exp. (%) Ag8392, Run 369780997














TABLE XF










General_screening_panel_v1.6










Tissue Name
A














Adipose
0.5



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.3



Melanoma* SK-MEL-5
0.4



Squamous cell carcinoma SCC-4
1.5



Testis Pool
1.3



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.2



Placenta
0.1



Uterus Pool
0.2



Ovarian ca. OVCAR-3
0.1



Ovarian ca. SK-OV-3
1.3



Ovarian ca. OVCAR-4
1.0



Ovarian ca. OVCAR-5
39.8



Ovarian ca. IGROV-1
21.0



Ovarian ca. OVCAR-8
3.4



Ovary
0.5



Breast ca. MCF-7
1.6



Breast ca. MDA-MB-231
0.1



Breast ca. BT 549
0.1



Breast ca. T47D
0.4



Breast ca. MDA-N
0.0



Breast Pool
0.6



Trachea
0.8



Lung
0.0



Fetal Lung
0.8



Lung ca. NCI-N417
7.9



Lung ca. LX-1
0.0



Lung ca. NCI-H146
1.3



Lung ca. SHP-77
6.2



Lung ca. A549
0.2



Lung ca. NCI-H526
3.0



Lung ca. NCI-H23
1.6



Lung ca. NCI-H460
0.5



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
17.9



Liver
0.0



Fetal Liver
0.3



Liver ca. HepG2
0.0



Kidney Pool
0.4



Fetal Kidney
0.2



Renal ca. 786-0
1.4



Renal ca. A498
21.6



Renal ca. ACHN
6.2



Renal ca. UO-31
0.6



Renal ca. TK-10
10.9



Bladder
1.2



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
6.6



Colon ca.* (SW480 met) SW620
0.8



Colon ca. HT29
0.3



Colon ca. HCT-116
15.5



Colon ca. CaCo-2
0.6



Colon cancer tissue
0.6



Colon ca. SW1116
4.0



Colon ca. Colo-205
0.1



Colon ca. SW-48
0.0



Colon Pool
0.8



Small Intestine Pool
0.4



Stomach Pool
0.5



Bone Marrow Pool
0.2



Fetal Heart
0.2



Heart Pool
0.1



Lymph Node Pool
0.8



Fetal Skeletal Muscle
0.8



Skeletal Muscle Pool
0.1



Spleen Pool
1.0



Thymus Pool
1.5



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.1



CNS cancer (neuro; met) SK-N-AS
2.5



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.9



CNS cancer (glio) SNB-19
19.5



CNS cancer (glio) SF-295
0.7



Brain (Amygdala) Pool
38.4



Brain (cerebellum)
100.0



Brain (fetal)
62.4



Brain (Hippocampus) Pool
36.9



Cerebral Cortex Pool
72.2



Brain (Substantia nigra) Pool
48.0



Brain (Thalamus) Pool
46.7



Brain (whole)
72.2



Spinal Cord Pool
3.2



Adrenal Gland
11.3



Pituitary gland Pool
2.8



Salivary Gland
0.5



Thyroid (female)
0.1



Pancreatic ca. CAPAN2
1.0



Pancreas Pool
0.3









Column A - Rel. Exp. (%) Ag1395, Run 277226632














TABLE XG










HASS Panel v1.0










Tissue Name
A














MCF-7 C1
7.5



MCF-7 C2
7.1



MCF-7 C3
4.0



MCF-7 C4
12.5



MCF-7 C5
9.1



MCF-7 C6
12.5



MCF-7 C7
5.2



MCF-7 C9
5.0



MCF-7 C10
8.2



MCF-7 C11
1.2



MCF-7 C12
5.1



MCF-7 C13
2.1



MCF-7 C15
3.6



MCF-7 C16
13.9



MCF-7 C17
8.9



T24 D1
0.1



T24 D2
0.1



T24 D3
0.1



T24 D4
0.4



T24 D5
0.2



T24 D6
0.1



T24 D7
0.2



T24 D9
0.1



T24 D10
0.0



T24 D11
0.1



T24 D12
0.0



T24 D13
0.2



T24 D15
0.1



T24 D16
0.0



T24 D17
0.1



CAPaN B1
13.0



CAPaN B2
11.6



CAPaN B3
5.0



CAPaN B4
12.7



CAPaN B5
18.4



CAPaN B6
11.2



CAPaN B7
3.8



CAPaN B8
3.7



CAPaN B9
7.5



CAPaN B10
20.0



CAPaN B11
26.8



CAPaN B12
14.6



CAPaN B13
5.1



CAPaN B14
5.9



CAPaN B15
8.0



CAPaN B16
13.8



CAPaN B17
13.7



U87-MG F1 (B)
0.0



U87-MG F2
0.0



U87-MG F3
0.1



U87-MG F4
0.0



U87-MG F5
0.0



U87-MG F6
0.1



U87-MG F7
0.0



U87-MG F8
0.1



U87-MG F9
0.1



U87-MG F10
0.0



U87-MG F11
0.0



U87-MG F12
0.0



U87-MG F13
0.0



U87-MG F14
0.0



U87-MG F15
0.0



U87-MG F16
0.0



U87-MG F17
0.1



LnCAP A1
0.9



LnCAP A2
0.5



LnCAP A3
0.9



LnCAP A4
1.0



LnCAP A5
1.2



LnCAP A6
0.4



LnCAP A7
0.7



LnCAP A8
1.2



LnCAP A9
1.1



LnCAP A10
1.0



LnCAP A11
1.6



LnCAP A12
0.0



LnCAP A13
0.4



LnCAP A14
1.2



LnCAP A15
0.3



LnCAP A16
1.1



LnCAP A17
1.3



Primary Astrocytes
0.2



Primary Renal Proximal Tubule Epithelial cell A2
1.5



Primary melanocytes A5
0.0



126443 - 341 medullo
0.0



126444 - 487 medullo
0.5



126445 - 425 medullo
0.0



126446 - 690 medullo
100.0



126447 - 54 adult glioma
0.8



126448 - 245 adult glioma
0.1



126449 - 317 adult glioma
0.1



126450 - 212 glioma
0.0



126451 - 456 glioma
0.1









Column A - Rel. Exp. (%) Ag1395, Run 268623664














TABLE XG










Panel 2D











Tissue Name
A
B















Normal Colon
4.2
1.9



CC Well to Mod Diff (ODO3866)
0.7
1.8



CC Margin (ODO3866)
0.0
1.0



CC Gr.2 rectosigmoid (ODO3868)
0.0
1.1



CC Margin (ODO3868)
0.0
0.0



CC Mod Diff (ODO3920)
0.0
1.2



CC Margin (ODO3920)
0.0
0.8



CC Gr.2 ascend colon (ODO3921)
0.9
2.3



CC Margin (ODO3921)
0.0
0.4



CC from Partial Hepatectomy
0.7
0.2



(ODO4309) Mets



Liver Margin (ODO4309)
0.0
0.9



Colon mets to lung (OD04451-01)
0.0
2.3



Lung Margin (OD04451-02)
0.8
0.0



Normal Prostate 6546-1
9.0
8.2



Prostate Cancer (OD04410)
0.0
3.5



Prostate Margin (OD04410)
2.0
1.7



Prostate Cancer (OD04720-01)
0.8
1.7



Prostate Margin (OD04720-02)
0.0
1.5



Normal Lung 061010
3.1
10.9



Lung Met to Muscle (ODO4286)
4.4
4.1



Muscle Margin (ODO4286)
0.0
0.5



Lung Malignant Cancer (OD03126)
2.2
2.5



Lung Margin (OD03126)
3.2
4.6



Lung Cancer (OD04404)
2.4
1.1



Lung Margin (OD04404)
3.3
4.2



Lung Cancer (OD04565)
0.0
1.6



Lung Margin (OD04565)
1.7
1.7



Lung Cancer (OD04237-01)
0.8
4.5



Lung Margin (OD04237-02)
3.8
7.1



Ocular Mel Met to Liver (ODO4310)
0.0
0.0



Liver Margin (ODO4310)
6.2
2.1



Melanoma Mets to Lung (OD04321)
0.8
0.0



Lung Margin (OD04321)
3.8
5.3



Normal Kidney
0.8
1.6



Kidney Ca, Nuclear grade 2 (OD04338)
1.2
2.8



Kidney Margin (OD04338)
0.0
1.8



Kidney Ca Nuclear grade 1/2
5.9
5.4



(OD04339)



Kidney Margin (OD04339)
0.0
0.0



Kidney Ca, Clear cell type (OD04340)
1.3
7.4



Kidney Margin (OD04340)
0.0
0.3



Kidney Ca, Nuclear grade 3 (OD04348)
0.0
2.1



Kidney Margin (OD04348)
0.8
0.8



Kidney Cancer (OD04622-01)
2.2
4.0



Kidney Margin (OD04622-03)
0.7
0.4



Kidney Cancer (OD04450-01)
0.0
0.4



Kidney Margin (OD04450-03)
0.0
0.0



Kidney Cancer 8120607
27.9
60.3



Kidney Margin 8120608
0.8
2.1



Kidney Cancer 8120613
0.8
1.7



Kidney Margin 8120614
0.7
0.7



Kidney Cancer 9010320
4.7
6.3



Kidney Margin 9010321
0.0
2.7



Normal Uterus
0.0
2.2



Uterus Cancer 064011
0.0
8.9



Normal Thyroid
8.7
1.2



Thyroid Cancer 064010
0.0
0.0



Thyroid Cancer A302152
0.0
2.5



Thyroid Margin A302153
1.1
0.8



Normal Breast
2.8
4.1



Breast Cancer (OD04566)
0.0
1.8



Breast Cancer (OD04590-01)
28.3
27.4



Breast Cancer Mets (OD04590-03)
13.3
14.2



Breast Cancer Metastasis (OD04655-05)
37.9
100.0



Breast Cancer 064006
12.0
19.2



Breast Cancer 1024
33.9
25.2



Breast Cancer 9100266
6.7
7.6



Breast Margin 9100265
0.5
9.2



Breast Cancer A209073
3.7
6.8



Breast Margin A209073
0.7
0.0



Normal Liver
0.0
2.6



Liver Cancer 064003
0.0
1.3



Liver Cancer 1025
0.4
2.0



Liver Cancer 1026
0.0
1.6



Liver Cancer 6004-T
1.6
3.3



Liver Tissue 6004-N
1.4
0.7



Liver Cancer 6005-T
0.8
0.8



Liver Tissue 6005-N
0.0
0.0



Normal Bladder
3.5
3.8



Bladder Cancer 1023
0.8
0.5



Bladder Cancer A302173
3.2
1.1



Bladder Cancer (OD04718-01)
3.8
2.3



Bladder Normal Adjacent (OD04718-03)
5.2
7.4



Normal Ovary
3.0
2.9



Ovarian Cancer 064008
3.2
2.9



Ovarian Cancer (OD04768-07)
3.5
4.6



Ovary Margin (OD04768-08)
0.9
2.2



Normal Stomach
2.7
3.7



Gastric Cancer 9060358
0.4
0.2



Stomach Margin 9060359
4.3
1.3



Gastric Cancer 9060395
3.0
1.2



Stomach Margin 9060394
2.5
1.0



Gastric Cancer 9060397
100.0
48.0



Stomach Margin 9060396
1.0
2.2



Gastric Cancer 064005
4.9
6.7









Column A - Rel. Exp. (%) Ag1395, Run 145711201






Column B - Rel. Exp. (%) Ag1395, Run 162599933














TABLE XI










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
0.0



TE671- Medulloblastoma
0.6



D283 Med- Medulloblastoma
0.5



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
0.6



SNB-78- Glioma
0.0



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
6.5



SK-N-SH- Neuroblastoma (metastasis)
10.3



SF-295- Glioblastoma
0.8



Cerebellum
46.0



Cerebellum
41.5



NCI-H292- Mucoepidermoid lung carcinoma
0.4



DMS-114- Small cell lung cancer
9.0



DMS-79- Small cell lung cancer
100.0



NCI-H146- Small cell lung cancer
4.2



NCI-H526- Small cell lung cancer
16.5



NCI-N417- Small cell lung cancer
22.8



NCI-H82- Small cell lung cancer
9.5



NCI-H157- Squamous cell lung cancer (metastasis)
0.0



NCI-H1155- Large cell lung cancer
27.7



NCI-H1299- Large cell lung cancer
0.0



NCI-H727- Lung carcinoid
14.5



NCI-UMC-11- Lung carcinoid
22.1



LX-1- Small cell lung cancer
0.2



Colo-205- Colon cancer
0.2



KM12- Colon cancer
0.0



KM20L2- Colon cancer
0.2



NCI-H716- Colon cancer
0.4



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
7.2



LS 174T- Colon adenocarcinoma
0.9



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
3.5



KATO III- Gastric carcinoma
0.3



NCI-SNU-16- Gastric carcinoma
2.1



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.0



MKN-45- Gastric carcinoma
2.9



NCI-N87- Gastric carcinoma
0.0



OVCAR-5- Ovarian carcinoma
11.2



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma
0.0



(metastasis)



ES-2- Ovarian clear cell carcinoma
0.2



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
0.0



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
0.0



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
5.3



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
6.3



Caki-2- Clear cell renal carcinoma
15.8



SW 839- Clear cell renal carcinoma
5.9



Rhabdoid kidney tumor
1.2



Hs766T- Pancreatic carcinoma (LN
0.0



metastasis)



CAPAN-1- Pancreatic adenocarcinoma (liver
27.7



metastasis)



SU86.86- Pancreatic carcinoma (liver
11.0



metastasis)



BxPC-3- Pancreatic adenocarcinoma
0.0



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.0



CFPAC-1- Pancreatic ductal adenocarcinoma
2.8



PANC-1- Pancreatic epithelioid ductal
0.2



carcinoma



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
0.2



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
0.3



A204- Rhabdomyosarcoma
1.0



HT-1080- Fibrosarcoma
0.0



MG-63- Osteosarcoma
0.2



SK-LMS-1- Leiomyosarcoma (vulva)
0.0



SJRH30- Rhabdomyosarcoma (met to bone
0.1



marrow)



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
0.0



DU 145- Prostate carcinoma (brain
0.0



metastasis)



MDA-MB-468- Breast adenocarcinoma
9.2



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
4.0









Column A - Rel. Exp. (%) Ag1395, Run 163477780














TABLE XJ










Panel 4D









Tissue Name
A
B












Secondary Th1 act
0.0
1.5


Secondary Th2 act
0.0
0.0


Secondary Tr1 act
0.0
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
0.0


Secondary Tr1 rest
0.0
0.0


Primary Th1 act
0.0
0.0


Primary Th2 act
0.0
0.0


Primary Tr1 act
1.6
0.0


Primary Th1 rest
1.7
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
1.3
0.0


CD45RA CD4 lymphocyte act
0.0
0.7


CD45RO CD4 lymphocyte act
1.2
0.0


CD8 lymphocyte act
0.0
0.0


Secondary CD8 lymphocyte rest
1.2
0.0


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
1.6
0.0


LAK cells rest
41.8
49.7


LAK cells IL-2
0.0
0.0


LAK cells IL-2 + IL-12
0.0
0.0


LAK cells IL-2 + IFN gamma
4.2
2.9


LAK cells IL-2 + IL-18
2.7
1.5


LAK cells PMA/ionomycin
63.3
100.0


NK Cells IL-2 rest
0.0
0.0


Two Way MLR 3 day
21.8
16.7


Two Way MLR 5 day
4.6
2.2


Two Way MLR 7 day
5.0
2.5


PBMC rest
3.6
9.5


PBMC PWM
0.0
97.9


PBMC PHA-L
2.9
2.7


Ramos (B cell) none
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0


B lymphocytes PWM
13.8
0.0


B lymphocytes CD40L and IL-4
0.0
0.0


EOL-1 dbcAMP
0.0
4.4


EOL-1 dbcAMP PMA/ionomycin
0.0
1.3


Dendritic cells none
31.9
34.4


Dendritic cells LPS
36.3
23.3


Dendritic cells anti-CD40
39.8
40.3


Monocytes rest
55.9
58.6


Monocytes LPS
13.3
11.3


Macrophages rest
93.3
96.6


Macrophages LPS
38.7
28.3


HUVEC none
0.0
4.0


HUVEC starved
2.6
0.0


HUVEC IL-1beta
0.0
1.4


HUVEC IFN gamma
1.5
6.3


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0


HUVEC IL-11
1.4
0.0


Lung Microvascular EC none
0.0
1.4


Lung Microvascular EC TNFalpha + IL-1beta
0.0
0.7


Microvascular Dermal EC none
11.0
5.5


Microsvasular Dermal EC TNFalpha + IL-1beta
7.6
17.4


Bronchial epithelium TNFalpha + IL1beta
0.0
0.0


Small airway epithelium none
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
1.1
1.9


Coronery artery SMC rest
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
1.5
0.0


Astrocytes TNFalpha + IL-1beta
0.0
1.2


KU-812 (Basophil) rest
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
2.6


CCD1106 (Keratinocytes) none
0.0
1.1


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
12.5
12.3


Liver cirrhosis
23.8
21.0


Lupus kidney
3.0
2.0


NCI-H292 none
1.1
5.8


NCI-H292 IL-4
2.1
6.4


NCI-H292 IL-9
3.8
0.0


NCI-H292 IL-13
0.0
4.2


NCI-H292 IFN gamma
7.1
7.1


HPAEC none
10.4
13.4


HPAEC TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast none
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast IL-4
0.0
0.0


Lung fibroblast IL-9
0.0
5.5


Lung fibroblast IL-13
1.3
0.0


Lung fibroblast IFN gamma
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
5.6


Dermal fibroblast IFN gamma
0.0
0.0


Dermal fibroblast IL-4
0.0
0.0


IBD Colitis 2
0.0
1.5


IBD Crohn's
1.4
0.0


Colon
21.3
19.2


Lung
100.0
95.3


Thymus
6.9
1.2


Kidney
8.8
3.8







Column A - Rel. Exp. (%) Ag1395, Run 140112952





Column B - Rel. Exp. (%) Ag1395, Run 142259379














TABLE XK










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
15.1



97476_Patient-07sk_skeletal muscle
0.0



97477_Patient-07ut_uterus
3.9



97478_Patient-07pl_placenta
0.0



99167_Bayer Patient 1
62.0



97482_Patient-08ut_uterus
8.2



97483_Patient-08pl_placenta
0.0



97486_Patient-09sk_skeletal muscle
0.0



97487_Patient-09ut_uterus
3.7



97488_Patient-09pl_placenta
0.0



97492_Patient-10ut_uterus
9.7



97493_Patient-10pl_placenta
0.0



97495_Patient-11go_adipose
17.2



97496_Patient-11sk_skeletal muscle
0.0



97497_Patient-11ut_uterus
14.7



97498_Patient-11pl_placenta
0.0



97500_Patient-12go_adipose
19.6



97501_Patient-12sk_skeletal muscle
0.0



97502_Patient-12ut_uterus
27.0



97503_Patient-12pl_placenta
1.6



94721_Donor 2 U - A_Mesenchymal Stem Cells
0.0



94722_Donor 2 U - B_Mesenchymal Stem Cells
0.0



94723_Donor 2 U - C_Mesenchymal Stem Cells
0.0



94709_Donor 2 AM - A_adipose
0.0



94710_Donor 2 AM - B_adipose
0.0



94711_Donor 2 AM - C_adipose
0.0



94712_Donor 2 AD - A_adipose
0.0



94713_Donor 2 AD - B_adipose
0.0



94714_Donor 2 AD - C_adipose
0.0



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
0.0



94730_Donor 3 AM - A_adipose
0.0



94731_Donor 3 AM - B_adipose
0.0



94732_Donor 3 AM - C_adipose
0.0



94733_Donor 3 AD - A_adipose
0.0



94734_Donor 3 AD - B_adipose
0.0



94735_Donor 3 AD - C_adipose
0.0



77138_Liver_HepG2 untreated
0.0



73556_Heart_Cardiac stromal cells (primary)
3.2



81735_Small Intestine
5.4



72409_Kidney_Proximal Convoluted Tubule
28.1



82685_Small intestine_Duodenum
0.0



90650_Adrenal_Adrenocortical adenoma
0.0



72410_Kidney_HRCE
42.6



72411_Kidney_HRE
100.0



73139_Uterus_Uterine smooth muscle cells
0.0









Column A - Rel. Exp. (%) Ag1395, Run 267095054







Ardais Breast1.0 Summary: Ag1395/Ag8392 Highest expression of this gene was detected in abreast cancer sample (CTs=25-27). Moderate to high levels of expression of this gene were seen in normal and cancer samples from breast. Expression of this gene was higher in a number of cancer samples. Therefore, therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule targeting this gene or gene product is useful in the treatment of breast cancer.


Ardais Panel 1.1 Summary: Ag1395/Ag8392 Highest expression of this gene was detected in normal adjacent lung tissue (CTs=29-30). Moderate to high levels of expression of this gene were seen in normal and cancer samples from lung. Expression of this gene was higher in a number of normal adjacent tissues. Therefore, therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule targeting this gene or gene product is useful in the treatment of lung cancer.


Ardais Prostate 1.0 Summary: Ag1395/Ag8392 Highest expression of this gene was detected in a prostate cancer sample (CTs=25). Moderate to high levels of expression of this gene were seen in normal and cancer samples from prostate. Expression of this gene was higher in a number of prostate cancer samples. Therefore, therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule targeting this gene or gene product is useful in the treatment of prostate cancer.


General_screening13 panel_v1.6 Summary: Ag1395 Highest expression of this gene was detected in cerebellum (CT=26). This gene showed a brain preferential expression, with high expression of this gene seen in all the regions of the brain examined including amygdala, cerebellum, hippocampus, cerebral cortex pool, substantia nigra, and thalamus. This gene encodes an UNC5H1 receptor, an ortholog of Caenorhabditis elegans UNC5, which is essential for dorsal guidance of pioneer axons and for the movement of cells away from the netrin ligand (Ackerman, S. L. Nature 386: 838-842). The family of Netrin ligands, netrin-1-2-3 and β-netrin, key regulators of neuronal cell migration and axonal guidance bind to the DCC (deleted in colorectal cancer) family and the UNC5 family (UNC5H1, UNC5H2 and UNC5H3). The DCC family of proteins is implicated in axon attraction and those of the UNC5 family in axon repulsion. Mice homozygous for the spontaneous rostral cerebellar malformation mutation (rcm(s)) homolog of UNC-5 exhibit cerebellar and midbrain defects as a result of abnormalities in the postnatal cerebellar neuronal migration. Modulation of the expression of this gene, expressed protein and/or use of antibodies/small molecule targeting this gene or gene product is useful in the treatment of cerbellar malformation and other brain related disorders including Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Significant expression of this gene was also seen in a number of cancer cell lines derived from pancreatic, lung, brain, colon, renal, and ovarian cancers. UNC5 receptors have been shown to create cellular states of dependence on their respective ligands by inhibiting apoptosis in the presence of ligand, but inducing apoptosis when unoccupied by ligand. UNC5H proteins are caspase substrates in vitro and caspase cleavage is required for cell death induction (Llambi F. EMBO J 20(11):2715-22). Based on the known role of these genes in cell migration and the expression of this gene cancer tissues and cell lines, including the metastatic cancer cell lines SW620 and SK-N-AS, the most likely mechanism for this gene is to increase the metastatic potential of tumor cells. When induced in endothelial cells, this gene will have a role in tumor induced endo cell migration and cell survival. The two roles might be interrelated as it is known that cells need to resist anoikis to survive during migration. Therefore, modulation of this gene, expressed protein and/or use of antibodies or small molecule targeting this gene or gene product is useful in the treatment of pancreatic, lung, brain, colon, renal, and ovarian cancers.


HASS Panel v1.0 Summary: Ag1395 Highest expression of this gene was detected in medullablastoma sample (CT=26.5). Low expression of this gene was also seen in a glioma sample. Therefore, therapeutic modulation of this gene or expressed protein is useful in the treatment of glioma and medulloblastoma.


In addition, significant expression of this gene was seen in samples from breast cancer MCF7 and pancreatic cancer CaPaN cell lines. The expression of this gene was not significantly changed by oxygen deprivation, acidic or a serum starved environment in the these two cell lines in this panel.


Panel 2D Summary: Ag1395 Highest expression of this gene was detected in a gastric cancer and metastatic breast cancer samples (CTs=29-31). Moderate to low levels of expression of this gene were also seen in number of kidney, breast and gastric cancer samples. Therefore, expression of this gene is useful as a marker to detect the presence of these cancers and therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule is useful in the treatment of breast, kidney and gastric cancers.


Panel 3D Summary: Ag1395 Highest expression of this gene was detected in a lung cancer cell line (CT=28.8). Moderate to low levels of expression of this gene were detected in cancer cell lines derived from metastatic neuroblastoma, glioblastoma, lung, gastric, colon, lymphoma, renal, pancreatic, breast and tongue cancers. Therefore, therapeutic modulation of this gene and/or expressed protein is useful in the treatment of these cancers.


Panel 4D Summary: Ag1395 Highest expression of this gene was detected in lung and PMA/ionomycin activated LAK cells (CTs=31). Moderate to low levels of expression of this gene were also seen in resting LAK cells, resting and activated dendritic cells, monocytes and macrophages, activated keratinocytes, liver cirrhosis and normal colon tissues. Expression of this gene in monocytes, LAK, macrophage and dendritic cells demonstrates that the expressed protein is important in differentiation and activation of these cell types. Therefore, regulating the expression of this gene or expressed protein will alter the types and levels of monocytic cells regulated by cytokine and chemokine production and T cell activation. Therapeutics modulation of this gene, encoded protein and/or use of antibodies or small molecule targeting this gene or gene product is useful in the treatment of liver cirrhosis, asthma, emphysema, inflammatory bowel disease, arthritis and psoriasis.


Panel 5 Islet Summary: Ag1395 Expression of this gene was limited to the kidney on this panel (CT=33.9). Therefore, modulation of this gene and/or expressed protein is useful in the treatment of kidney related disorders including systemic lupus erythematosus and glomerulonephritis.


Y. CG53944-02: Novel Membrane Protein


Expression of full-length physical clone CG53944-02 was assessed using the primer-probe sets Ag2639, Ag2640, and Ag2641, described in Tables YA, YB, and YC. Results of the RTQ-PCR runs are shown in Tables YD, YE, YF and YG.

TABLE YAProbe Name Ag2639SEQStartIDPrimersSequencesLengthPositionNoForward5′-ctcatcctcagcaacaacca-3′204061372ProbeTET-5′-cctggatgattgtgccgagacact-3′-244501373TAMRAReverse5′-taggagaggtcgaggtcctc-3′204751374









TABLE YB










Probe Name Ag2640
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctcatcctcagcaacaacca-3′
20
406
1375






Probe
TET-5′-cctggatgattgtgccgagacact-3′-
24
450
1376



TAMRA





Reverse
5′-tgtaggagaggtcgaggtcc-3′
20
477
1377
















TABLE YC










Probe Name Ag2641
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctcatcctcagcaacaacca-3′
20
406
1378






Probe
TET-5′-cctggatgattgtgccgagacact-3′-
24
450
1379



TAMRA





Reverse
5′-tgtaggagaggtcgaggtcc-3′
20
477
1380
















TABLE YD










CNS_neurodegeneration_v1.0










Tissue Name
A














AD 1 Hippo
55.5



AD 2 Hippo
79.0



AD 3 Hippo
35.8



AD 4 Hippo
63.3



AD 5 hippo
85.3



AD 6 Hippo
54.7



Control 2 Hippo
65.1



Control 4 Hippo
50.7



Control (Path) 3 Hippo
41.8



AD 1 Temporal Ctx
37.4



AD 2 Temporal Ctx
68.8



AD 3 Temporal Ctx
52.5



AD 4 Temporal Ctx
45.7



AD 5 Inf Temporal Ctx
63.7



AD 5 Sup Temporal Ctx
49.3



AD 6 Inf Temporal Ctx
58.6



AD 6 Sup Temporal Ctx
49.0



Control 1 Temporal Ctx
59.0



Control 2 Temporal Ctx
100.0



Control 3 Temporal Ctx
71.2



Control 4 Temporal Ctx
67.8



Control (Path) 1 Temporal Ctx
57.8



Control (Path) 2 Temporal Ctx
45.4



Control (Path) 3 Temporal Ctx
57.8



Control (Path) 4 Temporal Ctx
75.3



AD 1 Occipital Ctx
27.5



AD 2 Occipital Ctx (Missing)
2.0



AD 3 Occipital Ctx
36.3



AD 4 Occipital Ctx
32.5



AD 5 Occipital Ctx
37.4



AD 6 Occipital Ctx
49.7



Control 1 Occipital Ctx
55.1



Control 2 Occipital Ctx
92.0



Control 3 Occipital Ctx
57.8



Control 4 Occipital Ctx
46.3



Control (Path) 1 Occipital Ctx
69.7



Control (Path) 2 Occipital Ctx
63.3



Control (Path) 3 Occipital Ctx
51.8



Control (Path) 4 Occipital Ctx
82.9



Control 1 Parietal Ctx
39.5



Control 2 Parietal Ctx
54.3



Control 3 Parietal Ctx
67.4



Control (Path) 1 Parietal Ctx
84.7



Control (Path) 2 Parietal Ctx
56.6



Control (Path) 3 Parietal Ctx
47.0



Control (Path) 4 Parietal Ctx
79.6









Column A - Rel. Exp. (%) Ag2641, Run 206954924














TABLE YE










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
17.1



Pancreas
2.7



Pancreatic ca. CAPAN 2
3.9



Adrenal gland
4.4



Thyroid
5.9



Salivary gland
7.0



Pituitary gland
16.5



Brain (fetal)
12.4



Brain (whole)
12.4



Brain (amygdala)
31.0



Brain (cerebellum)
11.2



Brain (hippocampus)
100.0



Brain (substantia nigra)
3.6



Brain (thalamus)
19.3



Cerebral Cortex
24.1



Spinal cord
5.9



glio/astro U87-MG
12.9



glio/astro U-118-MG
17.1



astrocytoma SW1783
10.4



neuro*; met SK-N-AS
27.7



astrocytoma SF-539
9.9



astrocytoma SNB-75
19.9



glioma SNB-19
11.2



glioma U251
2.2



glioma SF-295
11.2



Heart (fetal)
5.4



Heart
1.0



Skeletal muscle (fetal)
15.3



Skeletal muscle
1.5



Bone marrow
1.2



Thymus
2.2



Spleen
7.4



Lymph node
2.4



Colorectal
1.5



Stomach
7.1



Small intestine
12.8



Colon ca. SW480
7.4



Colon ca.* SW620(SW480 met)
3.0



Colon ca. HT29
2.5



Colon ca. HCT-116
5.9



Colon ca. CaCo-2
5.0



Colon ca. tissue(ODO3866)
6.7



Colon ca. HCC-2998
23.2



Gastric ca.* (liver met) NCI-N87
9.9



Bladder
1.2



Trachea
12.6



Kidney
1.2



Kidney (fetal)
4.0



Renal ca. 786-0
1.9



Renal ca. A498
15.2



Renal ca. RXF 393
1.4



Renal ca. ACHN
2.9



Renal ca. UO-31
3.4



Renal ca. TK-10
1.6



Liver
2.9



Liver (fetal)
2.3



Liver ca. (hepatoblast) HepG2
5.3



Lung
7.3



Lung (fetal)
5.1



Lung ca. (small cell) LX-1
1.5



Lung ca. (small cell) NCI-H69
9.2



Lung ca. (s. cell var.) SHP-77
7.3



Lung ca. (large cell)NCI-H460
3.3



Lung ca. (non-sm. cell) A549
18.0



Lung ca. (non-s. cell) NCI-H23
7.0



Lung ca. (non-s. cell) HOP-62
7.3



Lung ca. (non-s. cl) NCI-H522
4.4



Lung ca. (squam.) SW 900
4.4



Lung ca. (squam.) NCI-H596
0.6



Mammary gland
9.5



Breast ca.* (pl. ef) MCF-7
6.3



Breast ca.* (pl. ef) MDA-MB-231
48.3



Breast ca.* (pl. ef) T47D
0.7



Breast ca. BT-549
13.9



Breast ca. MDA-N
8.5



Ovary
10.4



Ovarian ca. OVCAR-3
7.2



Ovarian ca. OVCAR-4
1.7



Ovarian ca. OVCAR-5
3.6



Ovarian ca. OVCAR-8
3.7



Ovarian ca. IGROV-1
2.5



Ovarian ca.* (ascites) SK-OV-3
3.5



Uterus
14.6



Placenta
3.4



Prostate
5.7



Prostate ca.* (bone met)PC-3
5.6



Testis
10.2



Melanoma Hs688(A).T
5.3



Melanoma* (met) Hs688(B).T
3.1



Melanoma UACC-62
2.0



Melanoma M14
2.8



Melanoma LOX IMVI
15.0



Melanoma* (met) SK-MEL-5
3.1



Adipose
2.0









Column A - Rel. Exp. (%) Ag2639, Run 157543474














TABLE YF










Panel 2D










Tissue Name
A














Normal Colon
43.5



CC Well to Mod Diff (ODO3866)
30.4



CC Margin (ODO3866)
18.8



CC Gr.2 rectosigmoid (ODO3868)
9.1



CC Margin (ODO3868)
3.6



CC Mod Diff (ODO3920)
22.8



CC Margin (ODO3920)
11.0



CC Gr.2 ascend colon (ODO3921)
40.1



CC Margin (ODO3921)
28.5



CC from Partial Hepatectomy (ODO4309) Mets
5.8



Liver Margin (ODO4309)
14.2



Colon mets to lung (OD04451-01)
15.8



Lung Margin (OD04451-02)
7.3



Normal Prostate 6546-1
17.0



Prostate Cancer (OD04410)
53.2



Prostate Margin (OD04410)
35.4



Prostate Cancer (OD04720-01)
31.4



Prostate Margin (OD04720-02)
36.9



Normal Lung 061010
29.1



Lung Met to Muscle (ODO4286)
8.7



Muscle Margin (ODO4286)
25.5



Lung Malignant Cancer (OD03126)
44.1



Lung Margin (OD03126)
23.3



Lung Cancer (OD04404)
33.2



Lung Margin (OD04404)
15.4



Lung Cancer (OD04565)
15.1



Lung Margin (OD04565)
6.0



Lung Cancer (OD04237-01)
14.8



Lung Margin (OD04237-02)
15.6



Ocular Mel Met to Liver (ODO4310)
13.5



Liver Margin (ODO4310)
17.6



Melanoma Mets to Lung (OD04321)
41.8



Lung Margin (OD04321)
28.3



Normal Kidney
43.2



Kidney Ca, Nuclear grade 2 (OD04338)
45.1



Kidney Margin (OD04338)
53.2



Kidney Ca Nuclear grade 1/2 (OD04339)
33.0



Kidney Margin (OD04339)
45.1



Kidney Ca, Clear cell type (OD04340)
15.3



Kidney Margin (OD04340)
36.3



Kidney Ca, Nuclear grade 3 (OD04348)
17.9



Kidney Margin (OD04348)
26.2



Kidney Cancer (OD04622-01)
42.9



Kidney Margin (OD04622-03)
15.3



Kidney Cancer (OD04450-01)
20.6



Kidney Margin (OD04450-03)
24.5



Kidney Cancer 8120607
44.1



Kidney Margin 8120608
34.4



Kidney Cancer 8120613
39.0



Kidney Margin 8120614
39.8



Kidney Cancer 9010320
24.8



Kidney Margin 9010321
41.5



Normal Uterus
12.7



Uterus Cancer 064011
39.8



Normal Thyroid
23.3



Thyroid Cancer 064010
21.3



Thyroid Cancer A302152
11.3



Thyroid Margin A302153
30.6



Normal Breast
45.1



Breast Cancer (OD04566)
16.6



Breast Cancer (OD04590-01)
81.8



Breast Cancer Mets (OD04590-03)
100.0



Breast Cancer Metastasis (OD04655-05)
72.7



Breast Cancer 064006
16.3



Breast Cancer 1024
44.8



Breast Cancer 9100266
28.1



Breast Margin 9100265
13.2



Breast Cancer A209073
44.1



Breast Margin A209073
24.1



Normal Liver
13.7



Liver Cancer 064003
15.1



Liver Cancer 1025
14.3



Liver Cancer 1026
23.2



Liver Cancer 6004-T
16.2



Liver Tissue 6004-N
7.2



Liver Cancer 6005-T
36.9



Liver Tissue 6005-N
10.8



Normal Bladder
23.7



Bladder Cancer 1023
19.9



Bladder Cancer A302173
12.4



Bladder Cancer (OD04718-01)
38.2



Bladder Normal Adjacent (OD04718-03)
11.8



Normal Ovary
38.2



Ovarian Cancer 064008
59.9



Ovarian Cancer (OD04768-07)
38.7



Ovary Margin (OD04768-08)
9.9



Normal Stomach
18.9



Gastric Cancer 9060358
5.2



Stomach Margin 9060359
25.5



Gastric Cancer 9060395
28.5



Stomach Margin 9060394
17.0



Gastric Cancer 9060397
49.7



Stomach Margin 9060396
22.8



Gastric Cancer 064005
18.3









Column A - Rel. Exp. (%) Ag2640, Run 157543604














TABLE YG










Panel 4D










Tissue Name
A














Secondary Th1 act
72.7



Secondary Th2 act
75.3



Secondary Tr1 act
52.9



Secondary Th1 rest
36.6



Secondary Th2 rest
45.7



Secondary Tr1 rest
57.0



Primary Th1 act
34.9



Primary Th2 act
31.6



Primary Tr1 act
32.5



Primary Th1 rest
90.8



Primary Th2 rest
50.3



Primary Tr1 rest
25.9



CD45RA CD4 lymphocyte act
79.6



CD45RO CD4 lymphocyte act
53.6



CD8 lymphocyte act
49.3



Secondary CD8 lymphocyte rest
78.5



Secondary CD8 lymphocyte act
14.0



CD4 lymphocyte none
25.3



2ry Th1/Th2/Tr1_anti-CD95 CH11
26.1



LAK cells rest
26.1



LAK cells IL-2
40.1



LAK cells IL-2 + IL-12
47.0



LAK cells IL-2 + IFN gamma
49.7



LAK cells IL-2 + IL-18
49.0



LAK cells PMA/ionomycin
14.8



NK Cells IL-2 rest
38.7



Two Way MLR 3 day
58.2



Two Way MLR 5 day
19.8



Two Way MLR 7 day
12.9



PBMC rest
23.2



PBMC PWM
89.5



PBMC PHA-L
35.4



Ramos (B cell) none
24.1



Ramos (B cell) ionomycin
93.3



B lymphocytes PWM
40.6



B lymphocytes CD40L and IL-4
14.8



EOL-1 dbcAMP
100.0



EOL-1 dbcAMP PMA/ionomycin
81.2



Dendritic cells none
5.5



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
6.0



Monocytes rest
3.1



Monocytes LPS
4.6



Macrophages rest
11.0



Macrophages LPS
1.6



HUVEC none
63.7



HUVEC starved
48.6



HUVEC IL-1beta
0.0



HUVEC IFN gamma
96.6



HUVEC TNF alpha + IFN gamma
51.4



HUVEC TNF alpha + IL4
93.3



HUVEC IL-11
57.4



Lung Microvascular EC none
87.7



Lung Microvascular EC TNFalpha + IL-1beta
82.9



Microvascular Dermal EC none
71.2



Microsvasular Dermal EC TNFalpha + IL-1beta
43.5



Bronchial epithelium TNFalpha + IL1beta
95.9



Small airway epithelium none
25.7



Small airway epithelium TNFalpha + IL-1beta
82.4



Coronery artery SMC rest
53.6



Coronery artery SMC TNFalpha + IL-1beta
81.2



Astrocytes rest
57.8



Astrocytes TNFalpha + IL-1beta
41.5



KU-812 (Basophil) rest
25.7



KU-812 (Basophil) PMA/ionomycin
30.8



CCD1106 (Keratinocytes) none
54.3



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
69.3



Liver cirrhosis
23.3



Lupus kidney
2.9



NCI-H292 none
36.1



NCI-H292 IL-4
67.8



NCI-H292 IL-9
62.0



NCI-H292 IL-13
90.1



NCI-H292 IFN gamma
82.4



HPAEC none
49.3



HPAEC TNF alpha + IL-1beta
45.7



Lung fibroblast none
44.8



Lung fibroblast TNF alpha + IL-1 beta
35.6



Lung fibroblast IL-4
69.3



Lung fibroblast IL-9
43.8



Lung fibroblast IL-13
45.1



Lung fibroblast IFN gamma
52.5



Dermal fibroblast CCD1070 rest
66.9



Dermal fibroblast CCD1070 TNF alpha
54.3



Dermal fibroblast CCD1070 IL-1 beta
71.2



Dermal fibroblast IFN gamma
84.1



Dermal fibroblast IL-4
58.2



IBD Colitis 2
3.1



IBD Crohn's
2.6



Colon
77.9



Lung
35.1



Thymus
41.2



Kidney
23.3









Column A - Rel. Exp. (%) Ag2641, Run 157543823







CNS_neurodegeneration_v1.0 Summary: Ag2641 This panel confirmed the expression of this gene at low levels in the brain in an independent group of individuals. This gene was found to be down-regulated in the temporal cortex of Alzheimer's disease patients patients when compared with non-demented controls (p=0.0003 when analyzed by Ancova, estimate of total cDNA loaded per well used asa covariate). Therefore, up-regulation of this gene or its protein product, or treatment with specific agonists for this receptor is of use in reversing the dementia, memory loss, and neuronal death associated with this disease.


Panel 1.3D Summary: Ag2639 Highest expression was seen in the hippocampus (CT=26.3). In addition, high to moderate levels of expression were seen in all regions of the CNS examined, including amygdala, hippocampus, substantia nigra, thalamus, and hypothalamus. This expression shows that therapeutic modulation of the expression or function of this gene is useful in the treatment of neurologic disorders, such as Alzheimer's disease, Parkinson's disease, schizophrenia, multiple sclerosis, stroke and epilepsy.


Panel 2D Summary: Ag2640 Highest expression was seen in a breast metastasis sample (CT=28.4), with moderate expression throughout this panel. This widespread expression demonstrates that this gene or gene product plays in cell growth and/or proliferation.


Panel 4D Summary: Ag2641 Highest expression was seen in an eosinophil cell line (CT=30). This gene was also expressed at moderate levels in a wide range of cell types of significance in the immune response in health and disease. These cells included members of the T-cell, B-cell, endothelial cell, macrophage/monocyte, and peripheral blood mononuclear cell family, as well as epithelial and fibroblast cell types from lung and skin, and normal tissues represented by colon, lung, thymus and kidney. This ubiquitous pattern of expression demonstrates that this gene product is involved in homeostatic processes for these and other cell types and tissues. This pattern was in agreement with the expression profile in Panel 2D and also shows a role for the gene or gene product in cell survival and proliferation. Therefore, modulation of the gene or its protein product with a functional therapeutic is useful for the alteration of functions associated with these cell types and for the improvement of the symptoms of patients suffering from autoimmune and inflammatory diseases such as asthma, allergies, inflammatory bowel disease, lupus erythematosus, psoriasis, rheumatoid arthritis, and osteoarthritis.


Z. CG5430804: Serpin


Expression of full-length physical clone CG54308-04 was assessed using the primer-probe sets Ag548 and Ag7880, described in Tables ZA and ZB. Results of the RTQ-PCR runs are shown in Tables ZC, ZD, ZE, ZF, and ZG.

TABLE ZAProbe Name Ag548SEQStartIDPrimersSequencesLengthPositionNoForward5′-tggagcagctcagaaaacatgt-3′229101381ProbeTET-5′-agaatcggtggtcctgtccttcccc-3′-259361382TAMRAReverse5′-catagctgtcttccagggtgaac-3′239631383









TABLE ZB










Probe Name Ag7880
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-tccagaggctctgtcgtttt-3′
20
277
1384






Probe
TET-5′-tccagagagctgtcagccagcacttt-3′-
26
241
1385



TAMRA





Reverse
5′-gaacctgacccttgtctgaaa-3′
21
208
1386
















TABLE ZC










General_screening_panel_v1.7










Tissue Name
A














Adipose
63.3



HUVEC
0.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
18.3



Melanoma (met) SK-MEL-5
0.0



Testis
7.9



Prostate ca. (bone met) PC-3
0.0



Prostate ca. DU145
0.0



Prostate pool
0.0



Uterus pool
100.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. (ascites) SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
8.1



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.0



Ovary
0.0



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT-549
0.0



Breast ca. T47D
0.0



Breast pool
0.0



Trachea
12.1



Lung
0.0



Fetal Lung
0.0



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Lung ca. DMS-114
0.0



Liver
0.0



Fetal Liver
0.0



Kidney pool
0.1



Fetal Kidney
0.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.0



Gastric ca. (liver met.) NCI-N87
0.0



Stomach
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca. (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon cancer tissue
0.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon
0.0



Small Intestine
0.0



Fetal Heart
0.0



Heart
0.0



Lymph Node pool 1
0.0



Lymph Node pool 2
0.0



Fetal Skeletal Muscle
6.7



Skeletal Muscle pool
0.0



Skeletal Muscle
0.0



Spleen
0.0



Thymus
7.5



CNS cancer (glio/astro) SF-268
0.0



CNS cancer (glio/astro) T98G
0.0



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala)
0.0



Brain (Cerebellum)
0.0



Brain (Fetal)
0.0



Brain (Hippocampus)
0.0



Cerebral Cortex pool
0.0



Brain (Substantia nigra)
0.0



Brain (Thalamus)
0.0



Brain (Whole)
0.0



Spinal Cord
0.0



Adrenal Gland
0.0



Pituitary Gland
0.0



Salivary Gland
0.0



Thyroid
0.0



Pancreatic ca. PANC-1
0.0



Pancreas pool
0.0









Column A - Rel. Exp. (%) Ag7880, Run 319066287














TABLE ZD










Panel 1.2










Tissue Name
A














Endothelial cells
0.0



Heart (Fetal)
0.0



Pancreas
0.0



Pancreatic ca. CAPAN 2
0.0



Adrenal Gland
0.0



Thyroid
0.0



Salivary gland
0.0



Pituitary gland
0.0



Brain (fetal)
0.0



Brain (whole)
0.0



Brain (amygdala)
0.0



Brain (cerebellum)
0.0



Brain (hippocampus)
0.0



Brain (thalamus)
0.0



Cerebral Cortex
0.0



Spinal cord
0.0



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.0



glioma U251
0.0



glioma SF-295
0.0



Heart
0.0



Skeletal Muscle
0.0



Bone marrow
0.0



Thymus
100.0



Spleen
0.0



Lymph node
0.0



Colorectal Tissue
0.0



Stomach
0.0



Small intestine
0.0



Colon ca. SW480
0.0



Colon ca.* SW620 (SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. Tissue (ODO3866)
0.0



Colon ca. HCC-2998
0.0



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.0



Trachea
0.0



Kidney
0.0



Kidney (fetal)
0.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
0.0



Lung (fetal)
0.0



Lung ca. (small cell) LX-1
0.0



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
0.0



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
0.0



Breast ca. MDA-N
0.0



Ovary
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca. (ascites) SK-OV-3
0.0



Uterus
0.0



Placenta
0.0



Prostate
0.0



Prostate ca.* (bone met) PC-3
0.0



Testis
2.6



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0









Column A - Rel. Exp. (%) Ag548, Run 122671023














TABLE ZE










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.0



Pancreas
0.0



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
17.4



Thyroid
0.0



Salivary gland
0.0



Pituitary gland
0.0



Brain (fetal)
0.0



Brain (whole)
21.3



Brain (amygdala)
0.0



Brain (cerebellum)
0.0



Brain (hippocampus)
0.0



Brain (substantia nigra)
0.0



Brain (thalamus)
0.0



Cerebral Cortex
0.0



Spinal cord
0.0



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.0



glioma U251
0.0



glioma SF-295
0.0



Heart (fetal)
0.0



Heart
0.0



Skeletal muscle (fetal)
2.1



Skeletal muscle
0.0



Bone marrow
0.0



Thymus
100.0



Spleen
0.0



Lymph node
0.0



Colorectal
9.7



Stomach
0.0



Small intestine
0.0



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
0.0



Colon ca. HCC-2998
0.0



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.0



Trachea
0.0



Kidney
0.0



Kidney (fetal)
0.0



Renal ca. 786-0
2.9



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
11.3



Lung (fetal)
0.0



Lung ca. (small cell) LX-1
0.0



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
14.0



Mammary gland
23.7



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
11.5



Breast ca. BT-549
0.0



Breast ca. MDA-N
22.8



Ovary
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0



Uterus
0.0



Placenta
0.0



Prostate
5.3



Prostate ca.* (bone met)PC-3
12.8



Testis
22.1



Melanoma Hs688(A).T
11.0



Melanoma* (met) Hs688(B).T
10.6



Melanoma UACC-62
0.0



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
0.0









Column A - Rel. Exp. (%) Ag548, Run 167658585














TABLE ZF










Panel 2D











Tissue Name
A
B















Normal Colon
0.0
0.0



CC Well to Mod Diff (ODO3866)
5.6
4.6



CC Margin (ODO3866)
3.3
3.1



CC Gr.2 rectosigmoid (ODO3868)
0.0
0.0



CC Margin (ODO3868)
0.0
0.0



CC Mod Diff (ODO3920)
0.0
0.0



CC Margin (ODO3920)
0.0
0.0



CC Gr.2 ascend colon (ODO3921)
0.0
0.0



CC Margin (ODO3921)
6.3
0.0



CC from Partial Hepatectomy
0.0
0.0



(ODO4309) Mets



Liver Margin (ODO4309)
0.0
0.0



Colon mets to lung (OD04451-01)
4.6
2.4



Lung Margin (OD04451-02)
2.9
0.0



Normal Prostate 6546-1
2.5
6.1



Prostate Cancer (OD04410)
0.0
0.0



Prostate Margin (OD04410)
3.1
0.0



Prostate Cancer (OD04720-01)
4.5
0.0



Prostate Margin (OD04720-02)
0.0
0.0



Normal Lung 061010
6.6
0.0



Lung Met to Muscle (ODO4286)
12.9
3.7



Muscle Margin (ODO4286)
2.8
0.0



Lung Malignant Cancer (OD03126)
2.8
0.0



Lung Margin (OD03126)
3.0
0.0



Lung Cancer (OD04404)
0.0
0.0



Lung Margin (OD04404)
0.0
0.0



Lung Cancer (OD04565)
17.3
6.1



Lung Margin (OD04565)
1.4
0.0



Lung Cancer (OD04237-01)
0.0
0.0



Lung Margin (OD04237-02)
2.9
0.0



Ocular Mel Met to Liver (ODO4310)
5.1
0.0



Liver Margin (ODO4310)
0.0
2.0



Melanoma Mets to Lung (OD04321)
0.0
0.0



Lung Margin (OD04321)
0.0
0.0



Normal Kidney
0.0
0.0



Kidney Ca, Nuclear grade 2 (OD04338)
0.0
0.0



Kidney Margin (OD04338)
0.0
1.0



Kidney Ca Nuclear grade 1/2 (OD04339)
0.0
0.0



Kidney Margin (OD04339)
0.0
0.0



Kidney Ca, Clear cell type (OD04340)
0.0
2.8



Kidney Margin (OD04340)
0.0
0.0



Kidney Ca, Nuclear grade 3 (OD04348)
0.0
0.0



Kidney Margin (OD04348)
0.0
0.0



Kidney Cancer (OD04622-01)
0.0
0.0



Kidney Margin (OD04622-03)
4.6
3.8



Kidney Cancer (OD04450-01)
0.0
4.5



Kidney Margin (OD04450-03)
0.0
2.3



Kidney Cancer 8120607
0.0
0.0



Kidney Margin 8120608
0.0
0.0



Kidney Cancer 8120613
1.6
2.6



Kidney Margin 8120614
0.0
0.0



Kidney Cancer 9010320
0.0
0.0



Kidney Margin 9010321
0.0
0.0



Normal Uterus
0.0
8.8



Uterus Cancer 064011
0.0
0.0



Normal Thyroid
2.8
2.7



Thyroid Cancer 064010
0.0
4.5



Thyroid Cancer A302152
1.8
0.0



Thyroid Margin A302153
0.0
0.0



Normal Breast
2.5
0.0



Breast Cancer (OD04566)
2.3
0.0



Breast Cancer (OD04590-01)
0.0
0.0



Breast Cancer Mets (OD04590-03)
0.0
0.0



Breast Cancer Metastasis (OD04655-05)
0.0
0.0



Breast Cancer 064006
0.0
0.0



Breast Cancer 1024
5.8
0.0



Breast Cancer 9100266
0.0
0.0



Breast Margin 9100265
0.0
0.0



Breast Cancer A209073
9.5
0.0



Breast Margin A209073
0.0
0.0



Normal Liver
0.0
3.0



Liver Cancer 064003
0.0
2.7



Liver Cancer 1025
0.0
0.0



Liver Cancer 1026
0.0
0.0



Liver Cancer 6004-T
0.0
0.0



Liver Tissue 6004-N
8.4
4.5



Liver Cancer 6005-T
0.0
0.0



Liver Tissue 6005-N
0.0
0.0



Normal Bladder
0.0
2.8



Bladder Cancer 1023
0.0
5.0



Bladder Cancer A302173
100.0
100.0



Bladder Cancer (OD04718-01)
0.0
0.0



Bladder Normal Adjacent (OD04718-03)
0.0
4.4



Normal Ovary
0.0
0.0



Ovarian Cancer 064008
0.0
0.0



Ovarian Cancer (OD04768-07)
0.0
0.0



Ovary Margin (OD04768-08)
0.0
0.0



Normal Stomach
0.0
0.0



Gastric Cancer 9060358
0.0
0.0



Stomach Margin 9060359
0.0
0.0



Gastric Cancer 9060395
0.0
0.0



Stomach Margin 9060394
0.0
0.0



Gastric Cancer 9060397
0.0
0.0



Stomach Margin 9060396
0.0
0.0



Gastric Cancer 064005
1.5
6.7









Column A - Rel. Exp. (%) Ag548, Run 145364354






Column B - Rel. Exp. (%) Ag548, Run 145506152














TABLE ZG










Panel 4D










Tissue Name
A
B
C













Secondary Th1 act
0.0
3.1
0.0


Secondary Th2 act
0.0
0.0
4.9


Secondary Tr1 act
0.0
0.0
0.0


Secondary Th1 rest
0.0
0.0
0.0


Secondary Th2 rest
0.0
0.0
0.0


Secondary Tr1 rest
0.0
7.1
0.0


Primary Th1 act
0.0
0.0
0.0


Primary Th2 act
0.0
1.1
0.0


Primary Tr1 act
0.0
0.0
0.0


Primary Th1 rest
0.0
0.0
0.0


Primary Th2 rest
0.0
0.0
0.0


Primary Tr1 rest
0.0
0.0
5.3


CD45RA CD4 lymphocyte act
0.0
0.0
0.0


CD45RO CD4 lymphocyte act
0.0
0.0
0.0


CD8 lymphocyte act
0.0
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0
0.0


CD4 lymphocyte none
0.0
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0
0.0


LAK cells rest
0.0
0.0
0.0


LAK cells IL-2
5.8
0.0
0.0


LAK cells IL-2 + IL-12
0.0
0.0
0.0


LAK cells IL-2 + IFN gamma
0.0
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0
0.0


NK Cells IL-2 rest
0.0
0.0
0.0


Two Way MLR 3 day
0.0
3.3
0.0


Two Way MLR 5 day
0.0
0.0
0.0


Two Way MLR 7 day
0.0
0.0
0.0


PBMC rest
0.0
0.0
0.0


PBMC PWM
0.0
0.0
0.0


PBMC PHA-L
0.0
0.0
0.0


Ramos (B cell) none
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0


B lymphocytes PWM
0.0
0.0
0.0


B lymphocytes CD40L and IL-4
0.0
0.0
0.0


EOL-1 dbcAMP
0.0
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0


Dendritic cells none
3.5
0.0
0.0


Dendritic cells LPS
0.0
0.0
5.4


Dendritic cells anti-CD40
0.0
0.0
0.0


Monocytes rest
0.0
0.0
0.0


Monocytes LPS
0.0
0.0
0.0


Macrophages rest
4.5
0.0
0.0


Macrophages LPS
2.4
8.0
0.0


HUVEC none
0.0
0.0
0.0


HUVEC starved
0.0
0.0
0.0


HUVEC IL-1beta
0.0
0.0
0.0


HUVEC IFN gamma
0.0
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0
0.0


HUVEC IL-11
0.0
0.0
0.0


Lung Microvascular EC none
0.0
2.9
0.0


Lung Microvascular EC TNFalpha +
0.0
0.0
0.0


IL-1beta


Microvascular Dermal EC none
0.0
0.0
0.0


Microsvasular Dermal EC
0.0
0.0
0.0


TNFalpha + IL-1beta


Bronchial epithelium TNFalpha +
6.5
0.0
0.0


IL1beta


Small airway epithelium none
13.4
13.6
8.8


Small airway epithelium TNFalpha +
100.0
73.2
95.9


IL-1beta


Coronery artery SMC rest
0.0
0.0
0.0


Coronery artery SMC TNFalpha +
0.0
0.0
0.0


IL-1beta


Astrocytes rest
0.0
0.0
0.0


Astrocytes TNFalpha + IL-1beta
0.0
0.0
5.5


KU-812 (Basophil) rest
0.0
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0
0.0


CCD1106 (Keratinocytes) none
0.0
3.4
0.0


CCD1106 (Keratinocytes) TNFalpha +
4.3
2.4
0.0


IL-1beta


Liver cirrhosis
22.5
21.0
4.1


Lupus kidney
0.0
0.0
0.0


NCI-H292 none
6.2
7.7
22.7


NCI-H292 IL-4
4.2
0.0
23.0


NCI-H292 IL-9
3.7
11.4
9.0


NCI-H292 IL-13
17.0
13.2
17.1


NCI-H292 IFN gamma
0.0
2.3
13.0


HPAEC none
0.0
0.0
0.0


HPAEC TNF alpha + IL-1 beta
8.4
0.0
0.0


Lung fibroblast none
6.8
0.0
0.0


Lung fibroblast TNF alpha +
0.0
0.0
6.6


IL-1 beta


Lung fibroblast IL-4
0.0
0.0
0.0


Lung fibroblast IL-9
0.0
0.0
0.0


Lung fibroblast IL-13
0.0
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
2.8
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
2.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.0
6.3


Dermal fibroblast IFN gamma
0.0
0.0
0.0


Dermal fibroblast IL-4
0.0
0.0
0.0


IBD Colitis 2
3.5
2.5
19.9


IBD Crohn's
0.0
13.3
0.0


Colon
0.0
0.0
0.0


Lung
0.0
0.0
0.0


Thymus
0.0
0.0
0.0


Kidney
87.1
100.0
100.0







Column A - Rel. Exp. (%) Ag548, Run 145909057





Column B - Rel. Exp. (%) Ag548, Run 145928351





Column C - Rel. Exp. (%) Ag548, Run 164886875







General_screening13 panel_v1.7 Summary: Ag7880 Prominent expressin of this gene was seen in uterus and adipose (CTs=30).


Panel 1.2 Summary: Ag548 This gene was expressed at a very low level in this panel. The highest level of expression was seen in thymus (CT=34.33). Thus, expression of this gene is useful as a marker to differentiate thymus tissues from other samples on this panel.


Panel 1.3D Summary: Ag548 This gene was expressed at a very low level in this panel. The highest level of expression was seen in thymus (CT=34.33). Thus, expression of this gene is useful as a marker to differentiate thymus tissues from other samples on this panel.


Panel 2D Summary: Ag548 The highest level of expression in this panel was detected in a bladder cancer sample (CTs=30). Thus, expression of the gene or its protein product is useful as a marker to differentiate between normal and cancerous bladder tissue and therapeutic modulation of the gene product using antibodies , chimeric molecules or small molecule inhibitors is effective in the treatment of bladder cancer.


Panel 4D Summary: Ag548 This transcript was induced in small airway epithelium activated with TNFalpha+IL-1beta and was present in normal kidney. The transcript encodes a putative serpin like molecule (serine proteinase inhibitor). Serpins participate in multiple biological processes and mutations that alter serpin function can result in pulmonary dysfunction including asthma and emphysema. Therapies designed with the protein encoded for by this transcript are important for the treatment of lung disorders such as asthma and emphysema.


AA. CG54764-02: CALGIZZARIN


Expression of gene CG54764-02 was assessed using the primer-probe sets Ag1212 and Ag1528, described in Tables AAA and AAB. Results of the RTQ-PCR runs are shown in Tables AAC, AAD, AAE, AAF, and AAG.

TABLE AAAProbe Name Ag1212SEQStartIDPrimersSequencesLengthPositionNoForward5′-gaccataacagctgcaaactct-3′221031387ProbeTET-5′-ttcatgaacactgtactggttgcctt-3′-261451388TAMRAReverse5′-agcccttctggttctttgtg-3′201711389









TABLE AAB










Probe Name Ag1528
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gaccataacagctgcaaactct-3′
22
103
1390






Probe
TET-5′-tgaacactgtactggttgccttcaca-3′-
26
149
1391



TAMRA





Reverse
5′-ccagagcccttctggttct-3′
19
176
1392
















TABLE AAC










CNS_neurodegeneration_v1.0










Tissue Name
A














AD 1 Hippo
15.9



AD 2 Hippo
18.7



AD 3 Hippo
10.6



AD 4 Hippo
24.5



AD 5 Hippo
92.0



AD 6 Hippo
13.4



Control 2 Hippo
6.8



Control 4 Hippo
8.7



Control (Path) 3 Hippo
6.6



AD 1 Temporal Ctx
30.8



AD 2 Temporal Ctx
39.8



AD 3 Temporal Ctx
40.9



AD 4 Temporal Ctx
54.0



AD 5 Inf Temporal Ctx
51.8



AD 5 Sup Temporal Ctx
33.0



AD 6 Inf Temporal Ctx
27.2



AD 6 Sup Temporal Ctx
57.8



Control 1 Temporal Ctx
21.6



Control 2 Temporal Ctx
9.2



Control 3 Temporal Ctx
44.4



Control 3 Temporal Ctx
19.5



Control (Path) 1 Temporal Ctx
54.3



Control (Path) 2 Temporal Ctx
74.2



Control (Path) 3 Temporal Ctx
9.1



Control (Path) 4 Temporal Ctx
75.3



AD 1 Occipital Ctx
31.0



AD 2 Occipital Ctx (Missing)
2.8



AD 3 Occipital Ctx
20.6



AD 4 Occipital Ctx
55.1



AD 5 Occipital Ctx
16.5



AD 6 Occipital Ctx
27.5



Control 1 Occipital Ctx
7.8



Control 2 Occipital Ctx
17.1



Control 3 Occipital Ctx
36.6



Control 4 Occipital Ctx
8.4



Control (Path) 1 Occipital Ctx
68.8



Control (Path) 2 Occipital Ctx
43.5



Control (Path) 3 Occipital Ctx
0.3



Control (Path) 4 Occipital Ctx
100.0



Control 1 Parietal Ctx
23.2



Control 2 Parietal Ctx
38.2



Control 3 Parietal Ctx
17.9



Control (Path) 1 Parietal Ctx
42.9



Control (Path) 2 Parietal Ctx
50.7



Control (Path) 3 Parietal Ctx
5.7



Control (Path) 4 Parietal Ctx
84.7









Column A - Rel. Exp. (%) Ag1212, Run 206992259














TABLE AAD










Panel 1.3D












Tissue Name
A
B
C
















Liver adenocarcinoma
17.1
16.2
11.8



Pancreas
0.0
0.0
0.0



Pancreatic ca. CAPAN 2
3.9
0.6
2.3



Adrenal gland
0.0
0.0
0.0



Thyroid
0.0
0.0
0.0



Salivary gland
0.0
0.0
1.4



Pituitary gland
9.8
11.1
4.5



Brain (fetal)
100.0
32.5
25.9



Brain (whole)
80.1
23.7
36.6



Brain (amygdala)
29.5
27.0
46.3



Brain (cerebellum)
99.3
17.3
18.9



Brain (hippocampus)
64.6
32.1
100.0



Brain (substantia nigra)
10.2
0.0
4.0



Brain (thalamus)
27.2
17.0
24.7



Cerebral Cortex
30.8
100.0
66.0



Spinal cord
2.6
0.6
1.2



glio/astro U87-MG
38.4
49.3
17.6



glio/astro U-118-MG
12.9
8.8
3.7



astrocytoma SW1783
8.4
7.3
9.7



neuro*; met SK-N-AS
1.4
4.8
5.6



astrocytoma SF-539
10.2
7.4
8.0



astrocytoma SNB-75
9.0
14.6
9.0



glioma SNB-19
0.0
0.8
1.5



glioma U251
13.7
7.1
4.4



glioma SF-295
2.2
0.0
0.0



Heart (fetal)
0.0
0.0
0.0



Heart
1.6
0.0
0.0



Skeletal muscle (fetal)
3.1
1.4
5.3



Skeletal muscle
0.0
0.0
0.0



Bone marrow
0.0
0.0
0.0



Thymus
0.0
0.0
0.0



Spleen
0.0
0.0
0.0



Lymph node
0.0
0.0
0.0



Colorectal
0.0
0.9
2.7



Stomach
0.0
0.5
1.3



Small intestine
7.2
0.0
1.4



Colon ca. SW480
0.0
0.0
1.2



Colon ca.* SW620(SW480 met)
0.0
1.1
0.0



Colon ca. HT29
0.0
1.1
0.0



Colon ca. HCT-116
0.0
0.0
0.0



Colon ca. CaCo-2
0.0
0.0
0.0



Colon ca. tissue(ODO3866)
0.0
2.8
1.2



Colon ca. HCC-2998
0.0
0.0
0.0



Gastric ca.* (liver met) NCI-N87
0.0
0.0
0.0



Bladder
4.6
4.4
2.7



Trachea
0.0
3.5
1.2



Kidney
0.0
0.0
0.0



Kidney (fetal)
0.0
1.2
0.0



Renal ca. 786-0
0.0
0.0
0.0



Renal ca. A498
6.7
12.2
6.7



Renal ca. RXF 393
0.0
0.0
0.0



Renal ca. ACHN
0.0
0.0
0.0



Renal ca. UO-31
7.3
6.6
2.1



Renal ca. TK-10
0.0
0.0
0.0



Liver
3.1
1.0
0.0



Liver (fetal)
0.0
0.0
0.0



Liver ca. (hepatoblast) HepG2
0.0
0.0
0.0



Lung
0.0
0.0
0.0



Lung (fetal)
0.0
0.0
0.0



Lung ca. (small cell) LX-1
0.0
0.0
0.0



Lung ca. (small cell) NCI-H69
1.8
9.4
6.1



Lung ca. (s. cell var.) SHP-77
2.1
0.0
0.0



Lung ca. (large cell)NCI-H460
0.0
0.0
0.0



Lung ca. (non-sm. cell) A549
0.0
3.5
4.2



Lung ca. (non-s. cell) NCI-H23
2.5
0.0
0.0



Lung ca. (non-s. cell) HOP-62
15.4
13.7
4.2



Lung ca. (non-s. cl) NCI-H522
3.6
12.5
9.3



Lung ca. (squam.) SW 900
0.0
0.0
0.0



Lung ca. (squam.) NCI-H596
21.3
20.9
21.0



Mammary gland
5.1
7.4
0.0



Breast ca.* (pl. ef) MCF-7
0.0
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0
0.0



Breast ca.* (pl. ef) T47D
0.0
2.0
0.0



Breast ca. BT-549
0.0
0.8
1.2



Breast ca. MDA-N
0.0
32.1
18.0



Ovary
0.0
0.0
0.0



Ovarian ca. OVCAR-3
40.1
37.4
18.6



Ovarian ca. OVCAR-4
0.0
0.0
2.6



Ovarian ca. OVCAR-5
5.4
0.0
1.3



Ovarian ca. OVCAR-8
4.4
1.2
4.3



Ovarian ca. IGROV-1
0.0
1.1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0
15.5
8.1



Uterus
0.0
0.0
0.0



Placenta
0.0
2.2
0.0



Prostate
2.6
0.0
0.0



Prostate ca.* (bone met)PC-3
10.1
1.1
0.7



Testis
3.0
1.4
4.1



Melanoma Hs688(A).T
6.2
37.6
12.8



Melanoma* (met) Hs688(B).T
18.8
54.7
14.0



Melanoma UACC-62
10.3
1.0
1.2



Melanoma M14
85.3
9.6
26.4



Melanoma LOX IMVI
23.2
34.9
57.0



Melanoma* (met) SK-MEL-5
9.6
0.0
14.6



Adipose
2.0
3.7
2.4









Column A - Rel. Exp. (%) Ag1212, Run 165519986






Column B - Rel. Exp. (%) Ag1528, Run 147327845






Column C - Rel. Exp. (%) Ag1528, Run 148390885














TABLE AAE










Panel 2D











Tissue Name
A
B















Normal Colon
7.4
8.5



CC Well to Mod Diff (ODO3866)
3.9
2.3



CC Margin (ODO3866)
0.0
2.7



CC Gr.2 rectosigmoid (ODO3868)
0.0
4.7



CC Margin (ODO3868)
0.0
0.0



CC Mod Diff (ODO3920)
0.0
0.0



CC Margin (ODO3920)
0.0
1.2



CC Gr.2 ascend colon (ODO3921)
0.0
0.0



CC Margin (ODO3921)
1.9
1.3



CC from Partial Hepatectomy
0.0
2.4



(ODO4309) Mets



Liver Margin (ODO4309)
0.0
1.3



Colon mets to lung (OD04451-01)
0.0
1.1



Lung Margin (OD04451-02)
0.0
0.0



Normal Prostate 6546-1
0.0
1.2



Prostate Cancer (OD04410)
0.0
0.0



Prostate Margin (OD04410)
4.1
0.0



Prostate Cancer (OD04720-01)
4.0
0.0



Prostate Margin (OD04720-02)
0.0
1.1



Normal Lung 061010
0.0
3.9



Lung Met to Muscle (ODO4286)
0.0
2.6



Muscle Margin (ODO4286)
8.7
0.0



Lung Malignant Cancer (OD03126)
0.0
0.0



Lung Margin (OD03126)
0.0
0.5



Lung Cancer (OD04404)
8.0
21.9



Lung Margin (OD04404)
0.0
0.0



Lung Cancer (OD04565)
3.2
1.0



Lung Margin (OD04565)
1.8
1.2



Lung Cancer (OD04237-01)
9.0
4.4



Lung Margin (OD04237-02)
0.0
0.0



Ocular Mel Met to Liver (ODO4310)
0.0
0.0



Liver Margin (ODO4310)
0.0
0.0



Melanoma Mets to Lung (OD04321)
10.7
9.5



Lung Margin (OD04321)
0.0
0.0



Normal Kidney
10.4
4.8



Kidney Ca, Nuclear grade 2 (OD04338)
0.0
0.0



Kidney Margin (OD04338)
0.0
0.0



Kidney Ca Nuclear grade 1/2 (OD04339)
0.0
0.0



Kidney Margin (OD04339)
0.0
0.0



Kidney Ca, Clear cell type (OD04340)
0.0
0.0



Kidney Margin (OD04340)
0.0
0.0



Kidney Ca, Nuclear grade 3 (OD04348)
0.0
1.1



Kidney Margin (OD04348)
3.4
0.6



Kidney Cancer (OD04622-01)
0.0
1.1



Kidney Margin (OD04622-03)
0.0
0.0



Kidney Cancer (OD04450-01)
0.0
0.0



Kidney Margin (OD04450-03)
0.0
6.7



Kidney Cancer 8120607
0.0
0.0



Kidney Margin 8120608
0.0
0.0



Kidney Cancer 8120613
0.0
0.0



Kidney Margin 8120614
1.2
0.5



Kidney Cancer 9010320
0.5
1.1



Kidney Margin 9010321
0.0
0.0



Normal Uterus
0.0
0.6



Uterus Cancer 064011
0.0
0.0



Normal Thyroid
1.8
0.0



Thyroid Cancer 064010
0.0
0.0



Thyroid Cancer A302152
5.0
0.0



Thyroid Margin A302153
0.0
0.0



Normal Breast
10.5
5.3



Breast Cancer (OD04566)
2.1
4.3



Breast Cancer (OD04590-01)
1.3
1.0



Breast Cancer Mets (OD04590-03)
0.0
0.0



Breast Cancer Metastasis (OD04655-05)
0.0
0.0



Breast Cancer 064006
17.3
8.7



Breast Cancer 1024
10.1
11.7



Breast Cancer 9100266
0.0
1.0



Breast Margin 9100265
11.0
1.9



Breast Cancer A209073
9.1
5.2



Breast Margin A209073
17.0
19.8



Normal Liver
0.0
0.0



Liver Cancer 064003
1.8
1.1



Liver Cancer 1025
1.9
1.1



Liver Cancer 1026
0.0
0.0



Liver Cancer 6004-T
0.0
0.0



Liver Tissue 6004-N
3.6
1.7



Liver Cancer 6005-T
0.0
0.0



Liver Tissue 6005-N
0.0
0.0



Normal Bladder
4.2
2.4



Bladder Cancer 1023
0.0
4.3



Bladder Cancer A302173
100.0
100.0



Bladder Cancer (OD04718-01)
1.7
12.4



Bladder Normal Adjacent (OD04718-03)
1.9
0.9



Normal Ovary
0.0
0.0



Ovarian Cancer 064008
0.0
9.4



Ovarian Cancer (OD04768-07)
0.0
0.0



Ovary Margin (OD04768-08)
0.0
0.0



Normal Stomach
0.0
0.9



Gastric Cancer 9060358
0.0
0.8



Stomach Margin 9060359
0.0
0.0



Gastric Cancer 9060395
1.3
0.0



Stomach Margin 9060394
0.0
0.0



Gastric Cancer 9060397
0.0
0.0



Stomach Margin 9060396
0.0
0.0



Gastric Cancer 064005
0.0
2.6









Column A - Rel. Exp. (%) Ag1528, Run 145158111






Column B - Rel. Exp. (%) Ag1528, Run 147327848














TABLE AAF










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
2.6



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
10.6



XF-498- CNS
81.8



SNB-78- Glioma
8.8



SF-268- Glioblastoma
11.9



T98G- Glioblastoma
1.8



SK-N-SH- Neuroblastoma (metastasis)
27.7



SF-295- Glioblastoma
0.0



Cerebellum
84.7



Cerebellum
7.1



NCI-H292- Mucoepidermoid lung carcinoma
1.0



DMS-114- Small cell lung cancer
0.0



DMS-79- Small cell lung cancer
38.4



NCI-H146- Small cell lung cancer
0.0



NCI-H526- Small cell lung cancer
24.5



NCI-N417- Small cell lung cancer
9.4



NCI-H82- Small cell lung cancer
20.0



NCI-H157- Squamous cell lung cancer
39.2



(metastasis)



NCI-H1155- Large cell lung cancer
0.0



NCI-H1299- Large cell lung cancer
60.7



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
0.0



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
0.0



KM12- Colon cancer
5.8



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
2.2



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
2.0



KATO III- Gastric carcinoma
68.8



NCI-SNU-16- Gastric carcinoma
0.0



NCI-SNU-1- Gastric carcinoma
13.8



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.0



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
0.0



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma
40.6



(metastasis)



ES-2- Ovarian clear cell carcinoma
29.7



Ramos- Stimulated with PMA/ionomycin 6 h
4.5



Ramos- Stimulated with PMA/ionomycin 14 h
9.4



MEG-01- Chronic myelogenous leukemia
1.9



(megokaryoblast)



Raji- Burkitt's lymphoma
9.8



Daudi- Burkitt's lymphoma
100.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
2.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
1.6



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
0.0



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
0.0



Hs766T- Pancreatic carcinoma (LN
30.8



metastasis)



CAPAN-1- Pancreatic adenocarcinoma (liver
6.8



metastasis)



SU86.86- Pancreatic carcinoma (liver
50.7



metastasis)



BxPC-3- Pancreatic adenocarcinoma
26.8



HPAC- Pancreatic adenocarcinoma
4.4



MIA PaCa-2- Pancreatic carcinoma
10.2



CFPAC-1- Pancreatic ductal adenocarcinoma
3.8



PANC-1- Pancreatic epithelioid ductal
12.7



carcinoma



T24- Bladder carcinma (transitional cell)
33.4



5637- Bladder carcinoma
24.1



HT-1197- Bladder carcinoma
19.9



UM-UC-3- Bladder carcinma (transitional cell)
5.1



A204- Rhabdomyosarcoma
0.0



HT-1080- Fibrosarcoma
95.3



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
35.8



SJRH30- Rhabdomyosarcoma (met to bone
13.6



marrow)



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
12.3



DU 145- Prostate carcinoma (brain
0.0



metastasis)



MDA-MB-468- Breast adenocarcinoma
5.1



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp. (%) Ag1212, Run 164038901














TABLE AAG










Panel 4D











Tissue Name
A
B
C
D














Secondary Th1 act
0.1
0.0
0.0
0.0


Secondary Th2 act
0.0
0.0
0.0
0.0


Secondary Tr1 act
0.0
0.1
0.0
0.0


Secondary Th1 rest
0.0
0.0
0.0
0.0


Secondary Th2 rest
0.0
0.0
0.0
0.0


Secondary Tr1 rest
0.0
0.0
0.0
0.1


Primary Th1 act
0.0
0.0
0.0
0.0


Primary Th2 act
0.0
0.0
0.0
0.0


Primary Tr1 act
0.0
0.0
0.0
0.0


Primary Th1 rest
0.0
0.0
0.0
0.0


Primary Th2 rest
0.0
0.0
0.0
0.0


Primary Tr1 rest
0.0
0.0
0.0
0.0


CD45RA CD4 lymphocyte act
0.7
0.6
0.8
0.8


CD45RO CD4 lymphocyte act
0.0
0.0
0.0
0.0


CD8 lymphocyte act
0.0
0.0
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0
0.0
0.9


CD4 lymphocyte none
0.0
0.0
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0
0.0
0.0


LAK cells rest
0.0
0.0
0.0
0.0


LAK cells IL-2
0.0
0.0
0.1
0.0


LAK cells IL-2 + IL-12
0.2
0.0
0.0
0.1


LAK cells IL-2 + IFN gamma
0.0
0.2
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.0
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0
0.0
0.0


NK Cells IL-2 rest
0.0
0.0
0.0
0.0


Two Way MLR 3 day
0.0
0.0
0.0
0.0


Two Way MLR 5 day
0.0
0.0
0.0
0.0


Two Way MLR 7 day
0.0
0.0
0.0
0.0


PBMC rest
0.0
0.0
0.0
0.0


PBMC PWM
0.0
1.1
0.2
0.0


PBMC PHA-L
0.0
0.0
0.0
0.1


Ramos (B cell) none
42.6
21.0
19.2
21.6


Ramos (B cell) ionomycin
100.0
100.0
100.0
100.0


B lymphocytes PWM
0.3
0.5
0.5
0.2


B lymphocytes CD40L and IL-4
3.1
4.0
4.8
7.2


EOL-1 dbcAMP
0.0
0.0
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0
0.0


Dendritic cells none
0.2
0.1
0.1
0.2


Dendritic cells LPS
0.0
0.0
0.0
0.0


Dendritic cells anti-CD40
0.1
0.0
0.0
0.0


Monocytes rest
0.1
0.0
0.0
0.0


Monocytes LPS
0.1
0.0
0.0
0.0


Macrophages rest
0.9
0.0
0.0
0.0


Macrophages LPS
0.1
0.0
0.0
6.0


HUVEC none
3.8
4.0
3.8
2.9


HUVEC starved
6.7
5.1
6.3
5.0


HUVEC IL-1beta
2.3
1.3
2.8
1.0


HUVEC IFN gamma
2.2
2.0
2.2
1.3


HUVEC TNF alpha + IFN gamma
0.3
0.6
0.9
0.8


HUVEC TNF alpha + IL4
1.0
1.3
2.3
1.4


HUVEC IL-11
2.1
1.9
3.0
2.6


Lung Microvascular EC none
6.8
5.1
4.0
3.9


Lung Microvascular EC TNFalpha +
1.9
2.0
3.6
9.8


IL-1beta


Microvascular Dermal EC none
8.4
6.0
4.6
7.2


Microsvasular Dermal EC TNFalpha +
1.6
3.5
2.5
3.6


IL-1beta


Bronchial epithelium TNFalpha +
1.6
0.0
0.5
0.0


IL1beta


Small airway epithelium none
0.2
0.5
0.2
0.3


Small airway epithelium TNFalpha +
2.8
1.9
1.8
2.2


IL-1beta


Coronery artery SMC rest
1.7
1.6
1.2
0.5


Coronery artery SMC TNFalpha +
0.6
0.9
1.0
0.2


IL-1beta


Astrocytes rest
0.9
2.0
0.6
0.8


Astrocytes TNFalpha + IL-1beta
0.7
0.2
0.5
0.7


KU-812 (Basophil) rest
0.1
0.0
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0
0.0
0.0


CCD1106 (Keratinocytes) none
3.0
4.5
4.1
3.5


CCD1106 (Keratinocytes) TNFalpha +
11.7
0.7
1.0
0.4


IL-1beta


Liver cirrhosis
1.5
1.0
0.7
0.1


Lupus kidney
0.0
0.0
0.0
0.0


NCI-H292 none
0.0
0.1
0.0
0.0


NCI-H292 IL-4
0.5
2.3
0.3
0.2


NCI-H292 IL-9
0.1
0.1
0.2
0.3


NCI-H292 IL-13
0.2
0.7
0.2
0.0


NCI-H292 IFN gamma
0.0
0.1
0.0
0.1


HPAEC none
7.6
8.7
8.2
7.5


HPAEC TNF alpha + IL-1 beta
2.0
2.7
3.0
2.0


Lung fibroblast none
0.3
0.2
0.0
0.2


Lung fibroblast TNF alpha +
0.0
0.1
0.0
0.0


IL-1 beta


Lung fibroblast IL-4
0.1
0.3
0.5
0.1


Lung fibroblast IL-9
0.3
1.0
0.6
0.1


Lung fibroblast IL-13
1.4
0.7
0.1
0.3


Lung fibroblast IFN gamma
0.0
0.4
0.2
0.3


Dermal fibroblast CCD1070 rest
11.4
8.8
8.7
4.9


Dermal fibroblast CCD1070 TNF alpha
6.7
8.1
9.2
8.5


Dermal fibroblast CCD1070 IL-1 beta
4.5
2.1
2.5
4.7


Dermal fibroblast IFN gamma
0.8
1.4
1.0
0.1


Dermal fibroblast IL-4
1.0
1.1
1.3
1.4


IBD Colitis 2
0.0
0.1
0.1
0.2


IBD Crohn's
0.2
0.0
0.0
0.0


Colon
0.0
0.0
0.1
0.0


Lung
0.0
0.1
0.0
0.0


Thymus
0.5
0.3
0.2
0.4


Kidney
0.1
0.1
0.0
0.0







Column A - Rel. Exp. (%) Ag1212, Run 140407622





Column B - Rel. Exp. (%) Ag1528, Run 147327849





Column C - Rel. Exp. (%) Ag1528, Run 148106604





Column D - Rel. Exp. (%) Ag1528, Run 162699795







CNS_neurodegeneration_v1.0 Summary: Ag1212 Highest expression of this gene, a homolog of calgizzarin, was seen in the hippocampus of a patient with Alzheimer's disease. The gene was also expressed in numerous regions associated with Alzheimer's neurodegeneration including the hippocampus, cortex and amygdala, as seen below in Panels 1.2 and 1.3D. Calgizzarin (S100C, S100A11) accumulation in the nucleus is associated with cellular senescence, which may be critical in aging and age-related disorders such as Alzheimer's disease. Agents that bind S100C are cardioprotective and have been proven useful in extending life. Agents that block the action or nuclear localization of this gene or gene product are useful in the treatment of Alzheimer's disease and are effective in prolonging lifespan.


Panel 1.3D Summary: Ag1212/Ag1528 Highest expression of this gene was detected in the cortex, the fetal brain, and the hippocampus (CTs=31-33), with moderate expression across many regions of the brain. In addition, a substantial number of samples derived from melanoma and brain cancer cell lines showed expression of this gene. Thus, the expression of this gene is useful as a marker to distinguish melanoma cell lines, brain cancer cell lines and brain tissue from other samples in the panel. Therapeutic modulation of this gene or gene product, through the use of small molecule drugs, antibodies or protein therapeutics, is of benefit in the treatment of melanoma or brain cancer.


Panel 2D Summary: Ag1528 Highest expression of this gene was observed in bladder cancer (CTs=31), with significant over-expression in the bladder cancer samples when compared to expression in the normal adjacent tissue. There was also substantial expression in a cluster of samples derived from breast tissue. Expression of this gene is useful as a marker to distinguish bladder cancer and breast tissue from other samples in the panel. Therapeutic modulation of the expression of this gene or this gene product, through the use of small molecule drugs, antibodies or protein therapeutics is of use in the treatment of bladder cancer or breast disease.


Panel 3D Summary: Ag1212 Highest expression of this gene was seen in a cell line derived from a lymphoma cell line (Daudi) (CT=31.2). Low but significant expression was also seen in cell lines derived from brain cancer and lung cancer, as is seen in Panel 1.3D.


Panel 4D Summary: Ag1212/Ag1528 Highest expression of this gene was seen in ionomycin treated Ramos B cells (CTs=26-28). Lower levels of expression were also seen in dermal fibroblasts, HUVEC endothelial cells, and keratinocytes. The expression in keratinocytes of this gene product, a homolog of calgizzarin, is consistent with Robinson (J. Biol. Chem. 272: 12035.) Therapeutic modulation of the expression or function of this gene or gene product, through the use antibodies or protein therapeutics, is beneficial in the treatment of disease states associated with hyperactivation of keratinocytes. Higher expression of the gene product in a B cell line compared to the expression in a primary B cell line shows a role for the protein encoded by this gene in cell growth and/or differentiation. Therapeutic modulation of the expression or function of this gene product, through the use antibodies is beneficial in the treatment of B cell lymphomas.


AB. CG55033-01 and CG55033-04 and CG55033-05: LRR Domain Containing Protein


Expression of gene CG55033-01 and variants CG55033-04 and CG55033-05 was assessed using the primer-probe sets Ag1094, Ag6719 and Ag6725, described in Tables ABA, ABB and ABC. Results of the RTQ-PCR runs are shown in Tables ABE, ABF, ABG and ABH. Please note that Ag6725 is specific for the CG55033-04 and CG55033-05 variants only.

TABLE ABAProbe Name Ag1094SEQStartIDPrimersSequencesLengthPositionNoForward5′-atggactggaaaacctggaa-3′203981393ProbeTET-5′-tcctgcaagcagataacaattttatcaca-3′-294191394TAMRAReverse5′-tgctaaaggcacttggttca-3′204531395









TABLE ABB










Probe Name Ag6719
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ggaaaatcattcaccactcaca-3′
22
1575
1396






Probe
TET-5′-acaaaaccacgaaccaatcaacagaa-3′-
26
1613
1397



TAMRA





Reverse
5′-tctgtacaatgagctggcatct-3′
22
1653
1398
















TABLE ABC










Probe Name Ag6725
















SEQ






Start
ID


Primers
Sequences
Length
Position
No















Forward
5′-gaagctgtggattcatctctttt-3′
23
3
1399






Probe
TET-5′-catctctccttgcctgtatatctttacactcc-
32
29
1400



TAMRA





Reverse
5′-ggatgagagcactggagtttg-3′
21
61
1401
















TABLE ABD










Ardais Panel v.1.0










Tissue Name
A














136799_Lung cancer(362)
0.1



136800_Lung NAT(363)
4.8



136813_Lung cancer(372)
37.4



136814_Lung NAT(373)
2.8



136815_Lung cancer(374)
19.9



136816_Lung NAT(375)
4.3



136791_Lung cancer(35A)
0.5



136795_Lung cancer(35E)
100.0



136797_Lung cancer(360)
0.2



136794_Lung NAT(35D)
5.0



136818_Lung NAT(377)
5.4



136787_lung cancer(356)
0.2



136788_Lung NAT(357)
0.7



136804_Lung cancer(369)
1.1



136805_Lung NAT(36A)
2.5



136806_Lung cancer(36B)
18.2



136807_Lung NAT(36C)
2.4



136789_lung cancer(358)
0.7



136802_Lung cancer(365)
0.1



136803_Lung cancer(368)
1.1



136811_Lung cancer(370)
4.5



136810_Lung NAT(36F)
32.1









Column A - Rel. Exp. (%) Ag1094, Run 263147830














TABLE ABE










General_screening_panel_v1.6










Tissue Name
A














Adipose
0.7



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.6



Squamous cell carcinoma SCC-4
7.4



Testis Pool
0.5



Prostate ca.* (bone met) PC-3
28.1



Prostate Pool
6.2



Placenta
0.0



Uterus Pool
0.3



Ovarian ca. OVCAR-3
0.4



Ovarian ca. SK-OV-3
0.4



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
10.3



Ovarian ca. IGROV-1
10.2



Ovarian ca. OVCAR-8
1.0



Ovary
0.0



Breast ca. MCF-7
0.8



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.9



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
1.2



Trachea
4.6



Lung
0.0



Fetal Lung
14.4



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
16.8



Lung ca. SHP-77
100.0



Lung ca. A549
2.7



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
12.9



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
3.2



Lung ca. NCI-H522
0.1



Liver
0.0



Fetal Liver
2.7



Liver ca. HepG2
0.0



Kidney Pool
0.4



Fetal Kidney
7.8



Renal ca. 786-0
0.0



Renal ca. A498
0.5



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.1



Gastric ca. (liver met.) NCI-N87
9.5



Gastric ca. KATO III
9.5



Colon ca. SW-948
1.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.4



Colon ca. HT29
15.5



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
0.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
1.0



Small Intestine Pool
0.9



Stomach Pool
1.1



Bone Marrow Pool
0.6



Fetal Heart
0.9



Heart Pool
0.2



Lymph Node Pool
0.5



Fetal Skeletal Muscle
0.6



Skeletal Muscle Pool
0.0



Spleen Pool
0.0



Thymus Pool
1.5



CNS cancer (glio/astro) U87-MG
0.2



CNS cancer (glio/astro) U-118-MG
0.7



CNS cancer (neuro; met) SK-N-AS
18.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
6.3



CNS cancer (glio) SNB-19
7.4



CNS cancer (glio) SF-295
68.3



Brain (Amygdala) Pool
0.6



Brain (cerebellum)
0.2



Brain (fetal)
0.7



Brain (Hippocampus) Pool
0.3



Cerebral Cortex Pool
0.1



Brain (Substantia nigra) Pool
0.1



Brain (Thalamus) Pool
0.8



Brain (whole)
0.1



Spinal Cord Pool
0.3



Adrenal Gland
0.0



Pituitary gland Pool
0.0



Salivary Gland
0.5



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
0.0









Column A - Rel. Exp. (%) Ag6719, Run 277223815














TABLE ABF










Panel 2D











Tissue Name
A
B















Normal Colon
12.4
11.4



CC Well to Mod Diff (ODO3866)
0.1
0.0



CC Margin (ODO3866)
1.0
1.5



CC Gr.2 rectosigmoid (ODO3868)
0.3
0.1



CC Margin (ODO3868)
0.4
0.2



CC Mod Diff (ODO3920)
0.1
0.1



CC Margin (ODO3920)
0.8
0.8



CC Gr.2 ascend colon (ODO3921)
2.4
2.2



CC Margin (ODO3921)
2.0
1.9



CC from Partial Hepatectomy
0.0
0.0



(ODO4309) Mets



Liver Margin (ODO4309)
0.2
0.3



Colon mets to lung (OD04451-01)
0.1
0.1



Lung Margin (OD04451-02)
0.9
0.6



Normal Prostate 6546-1
2.7
3.0



Prostate Cancer (OD04410)
1.5
1.4



Prostate Margin (OD04410)
6.5
8.0



Prostate Cancer (OD04720-01)
5.9
6.1



Prostate Margin (OD04720-02)
14.1
12.6



Normal Lung 061010
3.3
3.5



Lung Met to Muscle (ODO4286)
0.2
0.2



Muscle Margin (ODO4286)
0.0
0.0



Lung Malignant Cancer (OD03126)
7.9
6.0



Lung Margin (OD03126)
1.8
2.5



Lung Cancer (OD04404)
24.8
21.6



Lung Margin (OD04404)
1.8
1.7



Lung Cancer (OD04565)
0.7
1.2



Lung Margin (OD04565)
0.5
0.7



Lung Cancer (OD04237-01)
13.5
12.5



Lung Margin (OD04237-02)
1.4
1.0



Ocular Mel Met to Liver (ODO4310)
0.0
0.0



Liver Margin (ODO4310)
0.2
0.4



Melanoma Mets to Lung (OD04321)
0.4
0.2



Lung Margin (OD04321)
2.5
1.2



Normal Kidney
0.2
0.1



Kidney Ca, Nuclear grade 2 (OD04338)
0.0
0.0



Kidney Margin (OD04338)
0.2
0.2



Kidney Ca Nuclear grade 1/2 (OD04339)
0.0
0.0



Kidney Margin (OD04339)
0.1
0.0



Kidney Ca, Clear cell type (OD04340)
0.1
0.2



Kidney Margin (OD04340)
0.1
0.2



Kidney Ca, Nuclear grade 3 (OD04348)
0.0
0.0



Kidney Margin (OD04348)
0.0
0.1



Kidney Cancer (OD04622-01)
0.4
0.4



Kidney Margin (OD04622-03)
0.0
0.0



Kidney Cancer (OD04450-01)
0.0
0.0



Kidney Margin (OD04450-03)
0.0
0.1



Kidney Cancer 8120607
0.4
0.3



Kidney Margin 8120608
0.0
0.0



Kidney Cancer 8120613
0.0
0.0



Kidney Margin 8120614
0.0
0.0



Kidney Cancer 9010320
0.1
0.0



Kidney Margin 9010321
0.0
0.0



Normal Uterus
0.1
0.0



Uterus Cancer 064011
0.5
0.6



Normal Thyroid
0.5
0.4



Thyroid Cancer 064010
0.0
0.1



Thyroid Cancer A302152
0.1
0.1



Thyroid Margin A302153
0.2
0.1



Normal Breast
5.5
5.6



Breast Cancer (OD04566)
0.5
0.7



Breast Cancer (OD04590-01)
3.0
3.9



Breast Cancer Mets (OD04590-03)
1.4
1.7



Breast Cancer Metastasis (OD04655-05)
100.0
100.0



Breast Cancer 064006
1.7
2.0



Breast Cancer 1024
0.8
0.8



Breast Cancer 9100266
3.5
4.1



Breast Margin 9100265
3.5
4.2



Breast Cancer A209073
0.7
0.7



Breast Margin A209073
1.1
1.3



Normal Liver
2.0
1.7



Liver Cancer 064003
0.0
0.1



Liver Cancer 1025
0.3
0.3



Liver Cancer 1026
0.0
0.0



Liver Cancer 6004-T
0.3
0.1



Liver Tissue 6004-N
0.0
0.1



Liver Cancer 6005-T
0.0
0.0



Liver Tissue 6005-N
0.1
0.1



Normal Bladder
0.2
0.1



Bladder Cancer 1023
3.0
3.1



Bladder Cancer A302173
1.0
0.8



Bladder Cancer (OD04718-01)
0.0
0.1



Bladder Normal Adjacent (OD04718-03)
4.1
3.4



Normal Ovary
0.1
0.0



Ovarian Cancer 064008
1.6
1.4



Ovarian Cancer (OD04768-07)
0.0
0.0



Ovary Margin (OD04768-08)
0.1
0.0



Normal Stomach
1.0
1.7



Gastric Cancer 9060358
0.2
0.2



Stomach Margin 9060359
0.1
0.2



Gastric Cancer 9060395
0.4
0.7



Stomach Margin 9060394
0.4
0.4



Gastric Cancer 9060397
0.1
0.3



Stomach Margin 9060396
0.1
0.2



Gastric Cancer 064005
1.0
1.3









Column A - Rel. Exp. (%) Ag1094, Run 148015599






Column B - Rel. Exp. (%) Ag1094, Run 148572893














TABLE ABG










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
0.0



TE671- Medulloblastoma
0.2



D283 Med- Medulloblastoma
0.2



PFSK-1- Primitive Neuroectodermal
1.6



XF-498- CNS
30.4



SNB-78- Glioma
0.7



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
3.3



SK-N-SH- Neuroblastoma (metastasis)
22.4



SF-295- Glioblastoma
27.2



Cerebellum
6.7



Cerebellum
0.0



NCI-H292- Mucoepidermoid lung carcinoma
21.9



DMS-114- Small cell lung cancer
2.4



DMS-79- Small cell lung cancer
0.0



NCI-H146- Small cell lung cancer
100.0



NCI-H526- Small cell lung cancer
0.0



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
0.3



NCI-H157- Squamous cell lung cancer (metastasis)
0.0



NCI-H1155- Large cell lung cancer
65.1



NCI-H1299- Large cell lung cancer
0.0



NCI-H727- Lung carcinoid
13.8



NCI-UMC-11- Lung carcinoid
28.7



LX-1- Small cell lung cancer
0.7



Colo-205- Colon cancer
0.0



KM12- Colon cancer
0.1



KM20L2- Colon cancer
7.3



NCI-H716- Colon cancer
80.1



SW-48- Colon adenocarcinoma
0.3



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
0.6



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
0.0



KATO III- Gastric carcinoma
0.0



NCI-SNU-16- Gastric carcinoma
1.7



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.0



MKN-45- Gastric carcinoma
0.1



NCI-N87- Gastric carcinoma
2.4



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
2.8



HelaS3- Cervical adenocarcinoma
0.2



Ca Ski- Cervical epidermoid carcinoma
0.1



(metastasis)



ES-2- Ovarian clear cell carcinoma
0.0



Ramos- Stimulated with PMA/ionomycin 6 h
0.1



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
1.7



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.2



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.1



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
2.1



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
8.2



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
0.3



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
0.0



Hs766T- Pancreatic carcinoma (LN metastasis)
4.9



CAPAN-1- Pancreatic adenocarcinoma (liver
0.3



metastasis)



SU86.86- Pancreatic carcinoma (liver metastasis)
1.5



BxPC-3- Pancreatic adenocarcinoma
23.7



HPAC- Pancreatic adenocarcinoma
76.8



MIA PaCa-2- Pancreatic carcinoma
0.5



CFPAC-1- Pancreatic ductal adenocarcinoma
0.5



PANC-1- Pancreatic epithelioid ductal carcinoma
6.9



T24- Bladder carcinma (transitional cell)
4.1



5637- Bladder carcinoma
1.0



HT-1197- Bladder carcinoma
2.4



UM-UC-3- Bladder carcinma (transitional cell)
0.0



A204- Rhabdomyosarcoma
0.3



HT-1080- Fibrosarcoma
0.2



MG-63- Osteosarcoma
0.2



SK-LMS-1- Leiomyosarcoma (vulva)
0.0



SJRH30- Rhabdomyosarcoma (met to bone marrow)
0.2



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
0.4



DU 145- Prostate carcinoma (brain metastasis)
0.0



MDA-MB-468- Breast adenocarcinoma
1.3



SCC-4- Squamous cell carcinoma of tongue
0.2



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.3



CAL 27- Squamous cell carcinoma of tongue
1.6









Column A - Rel. Exp. (%) Ag1094, Run 165022642














TABLE ABH










Panel 4.1D









Tissue Name
A
B












Secondary Th1 act
0.0
0.0


Secondary Th2 act
0.0
0.0


Secondary Tr1 act
0.0
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
0.0


Secondary Tr1 rest
0.0
0.0


Primary Th1 act
0.0
0.0


Primary Th2 act
0.0
0.0


Primary Tr1 act
0.0
0.0


Primary Th1 rest
0.0
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
0.0


CD45RO CD4 lymphocyte act
0.0
0.0


CD8 lymphocyte act
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
0.0
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0


LAK cells rest
0.0
0.0


LAK cells IL-2
0.0
0.0


LAK cells IL-2 + IL-12
0.0
0.0


LAK cells IL-2 + IFN gamma
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.0


LAK cells PMA/ionomycin
0.0
0.0


NK Cells IL-2 rest
0.0
0.0


Two Way MLR 3 day
0.8
0.0


Two Way MLR 5 day
0.0
0.0


Two Way MLR 7 day
0.0
0.0


PBMC rest
0.0
0.0


PBMC PWM
0.0
0.0


PBMC PHA-L
0.0
0.0


Ramos (B cell) none
0.0
0.0


Ramos (B cell) ionomycin
0.6
0.5


B lymphocytes PWM
0.0
0.0


B lymphocytes CD40L and IL-4
0.0
0.0


EOL-1 dbcAMP
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0


Dendritic cells none
0.0
0.0


Dendritic cells LPS
0.0
0.0


Dendritic cells anti-CD40
0.0
0.0


Monocytes rest
0.0
0.0


Monocytes LPS
0.0
0.0


Macrophages rest
0.0
0.0


Macrophages LPS
0.0
0.0


HUVEC none
0.0
0.0


HUVEC starved
0.0
0.0


HUVEC IL-1beta
0.0
0.0


HUVEC IFN gamma
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0


HUVEC IL-11
0.0
0.0


Lung Microvascular EC none
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
0.0
0.0


Microvascular Dermal EC none
0.0
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.0


Bronchial epithelium TNFalpha + IL1beta
2.3
4.0


Small airway epithelium none
3.7
3.2


Small airway epithelium TNFalpha + IL-1beta
2.5
1.2


Coronery artery SMC rest
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
1.9
2.5


Astrocytes TNFalpha + IL-1beta
0.0
0.0


KU-812 (Basophil) rest
11.3
1.7


KU-812 (Basophil) PMA/ionomycin
61.1
100.0


CCD1106 (Keratinocytes) none
100.0
71.7


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
7.6
4.0


Liver cirrhosis
0.8
0.2


NCI-H292 none
7.2
6.4


NCI-H292 IL-4
4.6
6.8


NCI-H292 IL-9
25.0
8.4


NCI-H292 IL-13
9.7
7.8


NCI-H292 IFN gamma
2.8
1.9


HPAEC none
0.0
0.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast none
85.3
53.6


Lung fibroblast TNF alpha + IL-1 beta
35.1
31.0


Lung fibroblast IL-4
20.7
7.8


Lung fibroblast IL-9
2.8
7.3


Lung fibroblast IL-13
11.4
3.0


Lung fibroblast IFN gamma
52.1
38.2


Dermal fibroblast CCD1070 rest
0.3
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
1.0


Dermal fibroblast CCD1070 IL-1 beta
0.0
0.0


Dermal fibroblast IFN gamma
0.0
0.0


Dermal fibroblast IL-4
0.0
0.0


Dermal Fibroblasts rest
0.0
0.0


Neutrophils TNFa + LPS
0.0
0.0


Neutrophils rest
0.0
0.0


Colon
1.5
0.5


Lung
1.1
0.7


Thymus
1.4
0.0


Kidney
1.6
0.0







Column A - Rel. Exp. (%) Ag6719, Run 276596890





Column B - Rel. Exp. (%) Ag6725, Run 276846995







Ardais Panel v.1.0 Summary: Ag1094 Highest expression of this gene was seen in a lung cancer sample (CT=23.5). High to moderate expression of this gene was seen in a number of normal and cancerous samples from lung. Expression of this gene was higher in four cancer samples compared to normal lung samples. Expression of this gene is useful as a marker to detect the presence of lung cancer and therapeutic modulation of this gene or its protein product is useful in the treatment of lung cancer.


General_screening13 panel_v1.6 Summary: Ag6719 Highest expression of this gene was seen in a lung cancer SHP-77 cell line (CT=26.6). High expression of this gene was mainly seen in cancer cell lines derived from brain, lung, colon, gastric, ovarian and prostate cancers. Expression was higher in these cell lines compared to the normal tissues. Expression of this gene is useful as a marker to detect the presence of these cancers and also, therapeutic modulation of this gene or its protein product is useful in the treatment of brain, lung, colon, gastric, ovarian and prostate cancers.


Low expression of this gene was also seen in fetal lung, fetal kidney, and fetal heart. Expression was higher in these fetal tissues compared to expression in the corresponding adult tissues. This gene plays a role in early development of these tissue. Therapeutic modulation of this gene or its protein product is useful in the treatment of diseases related to development of these tissues.


This gene encodes a novel insulin-like growth factor binding protein acid labile subunit. Low expression of this gene was also seen in fetal brain and thalamus. Therefore, therapeutic modulation of this gene or gene product is useful in the treatment of neurological disorders related to thalamus and brain development. Insulin-like growth factor (IGF) has neuroprotective effects, and is currently under investigation as a biopharmaceutical for the treatment of amyotropic lateral sclerosis (Mewar R. J. Neurosci. Res 50:721-728, PMID: 9418960; Corse A. M., Neurobiol. Dis. 6:335-346). In serum, IGF is bound to both IGF-binding protein (IGFBP) and the acid labile subunit (IGFBP-ALS). In the brain, glia produce IGFBP; however the IGFBP-ALS has not been detected in the CNS. This gene represents the CNS equivalent of IGFBP-ALS. Because of the neuroprotective effects of IGF, therapeutic modulation of this gene or its protein product is useful in treating diseases in which neuronal death/degeneration occur such as amyotropic lateral sclerosis, multiple sclerosis, Alzheimer's disease, Parkinson's disease, Huntington's disease, spinocerebellar ataxia, or CNS injury such as stroke, head or spinal cord trauma.


Panel 2D Summary: Ag1094 Expression of this gene was highest in a metastatic breast cancer sample (CT=26-27). In addition, several other breast cancer and lung cancer samples showed increased expression when compared to their normal adjacent margin samples. This result was consistent with the results in the Ardais panel, and Panel 1.6 that both showed higher expression of this gene in cell lines and lung cancer tissue. Expression of this gene is useful as a marker to distinguish breast cancer or lung cancer tissue from their normal counterparts and is of diagnostic value. Therapeutic modulation of the this gene or its gene product, through the use of small molecule drugs or antibodies, is of benefit for treatment of breast or lung cancer.


Panel 3D Summary: Ag1094 Expression of this gene was highest in a small cell lung cancer cell line (CT=28.5). In addition, there was significant expression of this gene in other lung cancer cell lines as well as in several brain cancer and pancreatic cancer cell lines. These results were consistent with what is observed in the other panels. Expression of this gene is useful as a marker to distinguish lung, breast or pancreas cancer cell line samples from other tissues. Therapeutic modulation of this gene or gene product, through the use of small molecule drugs or antibodies, is of benefit for treatment of lung, breast or pancreatic cancer.


Panel 4.1D Summary: Ag6719/Ag6725 Highest expression of this gene was seen in activated basophils and resting keratinocytes (CTs=30-31). Significant expression of this gene was also seen in resting basophils, activated keratinocytes, activated NCI-H292, resting and activated lung fibroblasts. This expression pattern is in agreement with that seen in panel 4D. Please see panel 4D for further discussion on the utility of this gene. The protein encoded by this transcript is a homolog of insulin-like growth factor binding protein acid labile subunit, a component of the systemic insulin-like growth factor-binding protein (IGFBP) complex. This gene plays an important role in the biology of circulating IGFs. IGFs are involved in a wide array of cellular processes such as proliferation, prevention of apoptosis, and differentiation. This gene is a suitable target for a small molecule, antibody, or protein therapeutic to modulate locally the mitogenic effect of IGF and as a treatment of emphysema, COPD, or skin related disease.


AC. CG55117-04: Prominin-like Protein 1 Precursor


Expression of gene CG55117-04 was assessed using the primer-probe set Ag819, described in Table ACA. Results of the RTQ-PCR runs are shown in Tables ACB, ACC, ACD, ACE and ACF.

TABLE ACAProbe Name Ag819SEQStartIDPrimersSequencesLengthPositionNoForward5′-ggtccaacagggctatcaat-3′2010771402ProbeTET-5′-ccaaaccacgactgtcgtagcaggta-3′-2611281403TAMRAReverse5′-tggaattcaagacccttttga-3′2111541404









TABLE ACB










HASS Panel v1.0










Tissue Name
A














MCF-7 C1
0.0



MCF-7 C2
0.0



MCF-7 C3
0.0



MCF-7 C4
0.0



MCF-7 C5
0.1



MCF-7 C6
0.0



MCF-7 C7
0.0



MCF-7 C9
0.0



MCF-7 C10
0.0



MCF-7 C11
0.0



MCF-7 C12
0.1



MCF-7 C13
0.0



MCF-7 C15
0.0



MCF-7 C16
0.0



MCF-7 C17
0.0



T24 D1
0.0



T24 D2
0.0



T24 D3
0.1



T24 D4
0.1



T24 D5
0.1



T24 D6
0.0



T24 D7
0.0



T24 D9
0.0



T24 D10
0.0



T24 D11
0.0



T24 D12
0.0



T24 D13
0.0



T24 D15
0.0



T24 D16
0.0



T24 D17
0.0



CAPaN B1
66.9



CAPaN B2
50.7



CAPaN B3
11.8



CAPaN B4
42.3



CAPaN B5
45.4



CAPaN B6
73.7



CAPaN B7
27.7



CAPaN B8
17.6



CAPaN B9
67.4



CAPaN B10
100.0



CAPaN B11
53.2



CAPaN B12
55.9



CAPaN B13
28.7



CAPaN B14
15.3



CAPaN B15
39.0



CAPaN B16
44.8



CAPaN B17
36.9



U87-MG F1 (B)
0.1



U87-MG F2
0.0



U87-MG F3
0.0



U87-MG F4
0.0



U87-MG F5
0.1



U87-MG F6
0.0



U87-MG F7
0.3



U87-MG F8
0.2



U87-MG F9
0.3



U87-MG F10
0.1



U87-MG F11
0.0



U87-MG F12
0.0



U87-MG F13
0.7



U87-MG F14
0.0



U87-MG F15
0.5



U87-MG F16
0.1



U87-MG F17
0.1



LnCAP A1
0.0



LnCAP A2
0.0



LnCAP A3
0.0



LnCAP A4
0.0



LnCAP A5
0.1



LnCAP A6
0.1



LnCAP A7
0.0



LnCAP A8
0.1



LnCAP A9
0.0



LnCAP A10
0.0



LnCAP A11
0.0



LnCAP A12
0.0



LnCAP A13
0.0



LnCAP A14
0.0



LnCAP A15
0.0



LnCAP A16
0.0



LnCAP A17
0.0



Primary Astrocytes
37.6



Primary Renal Proximal Tubule Epithelial cell A2
38.2



Primary melanocytes A5
0.1



126443 - 341 medullo
0.4



126444 - 487 medullo
4.8



126445 - 425 medullo
0.0



126446 - 690 medullo
1.8



126447 - 54 adult glioma
0.0



126448 - 245 adult glioma
1.5



126449 - 317 adult glioma
10.7



126450 - 212 glioma
5.9



126451 - 456 glioma
60.7









Column A - Rel. Exp. (%) Ag819, Run 268362651














TABLE ACC










Panel 1.2











Tissue Name
A
B















Endothelial cells
0.0
0.0



Heart (Fetal)
0.4
0.8



Pancreas
43.8
48.3



Pancreatic ca. CAPAN 2
8.1
17.9



Adrenal Gland
0.2
0.2



Thyroid
11.8
12.9



Salivary gland
63.3
63.7



Pituitary gland
0.9
0.5



Brain (fetal)
37.1
41.5



Brain (whole)
4.6
6.3



Brain (amygdala)
1.5
2.3



Brain (cerebellum)
0.9
1.5



Brain (hippocampus)
3.4
4.0



Brain (thalamus)
1.9
2.6



Cerebral Cortex
1.2
2.7



Spinal cord
1.2
1.9



glio/astro U87-MG
0.0
0.0



glio/astro U-118-MG
0.0
0.0



astrocytoma SW1783
0.0
0.0



neuro*; met SK-N-AS
0.3
0.1



astrocytoma SF-539
0.0
0.0



astrocytoma SNB-75
0.0
0.0



glioma SNB-19
0.0
0.1



glioma U251
0.0
0.1



glioma SF-295
0.0
0.0



Heart
8.1
9.5



Skeletal Muscle
2.6
3.7



Bone marrow
0.6
1.2



Thymus
0.0
0.1



Spleen
0.5
0.0



Lymph node
1.4
0.2



Colorectal Tissue
0.3
1.8



Stomach
10.7
23.3



Small intestine
10.4
18.9



Colon ca. SW480
0.0
0.0



Colon ca.* SW620 (SW480 met)
9.0
11.7



Colon ca. HT29
32.5
40.9



Colon ca. HCT-116
5.9
7.9



Colon ca. CaCo-2
100.0
100.0



Colon ca. Tissue (ODO3866)
4.7
5.4



Colon ca. HCC-2998
2.5
3.0



Gastric ca.* (liver met) NCI-N87
0.0
0.2



Bladder
39.2
49.7



Trachea
29.7
34.4



Kidney
27.4
25.7



Kidney (fetal)
17.7
19.1



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.0
0.1



Renal ca. RXF 393
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
1.0
1.6



Renal ca. TK-10
0.0
0.0



Liver
8.0
3.3



Liver (fetal)
2.8
2.7



Liver ca. (hepatoblast) HepG2
12.8
20.2



Lung
5.7
4.2



Lung (fetal)
9.5
7.4



Lung ca. (small cell) LX-1
39.0
33.4



Lung ca. (small cell) NCI-H69
7.4
10.5



Lung ca. (s. cell var.) SHP-77
0.5
0.6



Lung ca. (large cell)NCI-H460
0.0
0.0



Lung ca. (non-sm. cell) A549
0.0
0.1



Lung ca. (non-s. cell) NCI-H23
0.0
0.0



Lung ca. (non-s. cell) HOP-62
0.1
0.2



Lung ca. (non-s. cl) NCI-H522
0.0
0.1



Lung ca. (squam.) SW 900
0.6
0.8



Lung ca. (squam.) NCI-H596
14.6
22.1



Mammary gland
33.0
46.3



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0



Breast ca.* (pl. ef) T47D
0.8
1.3



Breast ca. BT-549
0.0
0.0



Breast ca. MDA-N
0.4
0.6



Ovary
4.6
0.2



Ovarian ca. OVCAR-3
3.3
4.0



Ovarian ca. OVCAR-4
27.9
54.0



Ovarian ca. OVCAR-5
37.4
51.1



Ovarian ca. OVCAR-8
0.0
0.1



Ovarian ca. IGROV-1
3.2
5.5



Ovarian ca. (ascites) SK-OV-3
0.0
0.0



Uterus
1.4
1.2



Placenta
23.2
22.5



Prostate
2.6
2.7



Prostate ca.* (bone met) PC-3
0.0
0.0



Testis
19.8
21.9



Melanoma Hs688(A).T
1.7
0.0



Melanoma* (met) Hs688(B).T
0.7
0.0



Melanoma UACC-62
1.8
1.7



Melanoma M14
0.1
0.2



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.5
1.0









Column A - Rel. Exp. (%) Ag819, Run 118349021






Column B - Rel. Exp. (%) Ag819, Run 120989791














TABLE ACD










Panel 2D











Tissue Name
A
B















Normal Colon
17.0
20.7



CC Well to Mod Diff (ODO3866)
0.9
5.3



CC Margin (ODO3866)
9.5
6.0



CC Gr.2 rectosigmoid (ODO3868)
5.8
3.9



CC Margin (ODO3868)
0.0
0.2



CC Mod Diff (ODO3920)
0.7
0.9



CC Margin (ODO3920)
2.8
2.1



CC Gr.2 ascend colon (ODO3921)
26.2
37.4



CC Margin (ODO3921)
4.4
7.0



CC from Partial Hepatectomy
13.1
20.4



(ODO4309) Mets



Liver Margin (ODO4309)
0.1
0.2



Colon mets to lung (OD04451-01)
8.5
6.2



Lung Margin (OD04451-02)
2.1
1.7



Normal Prostate 6546-1
1.2
0.3



Prostate Cancer (OD04410)
0.5
0.7



Prostate Margin (OD04410)
0.8
0.6



Prostate Cancer (OD04720-01)
0.4
0.4



Prostate Margin (OD04720-02)
2.0
2.0



Normal Lung 061010
4.6
4.7



Lung Met to Muscle (ODO4286)
0.0
0.0



Muscle Margin (ODO4286)
0.2
0.3



Lung Malignant Cancer (OD03126)
8.7
6.5



Lung Margin (OD03126)
1.4
1.6



Lung Cancer (OD04404)
0.1
0.2



Lung Margin (OD04404)
3.1
1.2



Lung Cancer (OD04565)
0.2
0.1



Lung Margin (OD04565)
1.0
0.9



Lung Cancer (OD04237-01)
100.0
74.7



Lung Margin (OD04237-02)
1.7
1.5



Ocular Mel Met to Liver (ODO4310)
0.1
0.3



Liver Margin (ODO4310)
0.6
0.2



Melanoma Mets to Lung (OD04321)
14.8
12.2



Lung Margin (OD04321)
1.7
1.5



Normal Kidney
23.2
17.4



Kidney Ca, Nuclear grade 2 (OD04338)
4.2
5.1



Kidney Margin (OD04338)
8.0
11.3



Kidney Ca Nuclear grade 1/2 (OD04339)
65.5
69.3



Kidney Margin (OD04339)
6.7
6.0



Kidney Ca, Clear cell type (OD04340)
0.1
0.1



Kidney Margin (OD04340)
13.8
12.0



Kidney Ca, Nuclear grade 3 (OD04348)
0.0
0.0



Kidney Margin (OD04348)
9.2
6.3



Kidney Cancer (OD04622-01)
0.7
0.4



Kidney Margin (OD04622-03)
1.1
1.2



Kidney Cancer (OD04450-01)
32.5
24.5



Kidney Margin (OD04450-03)
22.1
16.0



Kidney Cancer 8120607
4.4
4.2



Kidney Margin 8120608
3.9
2.0



Kidney Cancer 8120613
0.1
0.0



Kidney Margin 8120614
1.2
0.8



Kidney Cancer 9010320
7.7
8.0



Kidney Margin 9010321
7.8
6.0



Normal Uterus
0.0
0.0



Uterus Cancer 064011
24.1
18.8



Normal Thyroid
4.7
2.4



Thyroid Cancer 064010
4.0
2.2



Thyroid Cancer A302152
0.1
0.0



Thyroid Margin A302153
2.9
2.7



Normal Breast
16.6
7.4



Breast Cancer (OD04566)
0.6
0.4



Breast Cancer (OD04590-01)
0.8
0.5



Breast Cancer Mets (OD04590-03)
0.0
0.0



Breast Cancer Metastasis (OD04655-05)
0.1
0.0



Breast Cancer 064006
15.7
11.7



Breast Cancer 1024
12.1
11.6



Breast Cancer 9100266
1.2
0.6



Breast Margin 9100265
3.0
2.0



Breast Cancer A209073
6.5
4.6



Breast Margin A209073
25.0
9.0



Normal Liver
0.6
0.5



Liver Cancer 064003
0.0
0.0



Liver Cancer 1025
0.2
0.3



Liver Cancer 1026
0.2
0.1



Liver Cancer 6004-T
0.2
0.1



Liver Tissue 6004-N
1.6
1.7



Liver Cancer 6005-T
0.1
0.2



Liver Tissue 6005-N
0.0
0.2



Normal Bladder
14.8
18.7



Bladder Cancer 1023
6.9
6.4



Bladder Cancer A302173
0.2
0.1



Bladder Cancer (OD04718-01)
0.1
0.1



Bladder Normal Adjacent (OD04718-03)
0.2
0.4



Normal Ovary
0.1
0.0



Ovarian Cancer 064008
68.8
100.0



Ovarian Cancer (OD04768-07)
0.5
1.0



Ovary Margin (OD04768-08)
0.0
0.1



Normal Stomach
5.0
4.5



Gastric Cancer 9060358
2.5
2.6



Stomach Margin 9060359
5.6
7.0



Gastric Cancer 9060395
1.7
1.3



Stomach Margin 9060394
3.4
6.4



Gastric Cancer 9060397
26.1
39.8



Stomach Margin 9060396
2.7
2.7



Gastric Cancer 064005
15.5
22.1









Column A - Rel. Exp. (%) Ag819, Run 144794769






Column B - Rel. Exp. (%) Ag819, Run 146791894














TABLE ACE










Panel 3D








Tissue Name
A











Daoy- Medulloblastoma
0.9


TE671- Medulloblastoma
1.1


D283 Med- Medulloblastoma
100.0


PFSK-1- Primitive Neuroectodermal
0.0


XF-498- CNS
0.1


SNB-78- Glioma
0.0


SF-268- Glioblastoma
0.0


T98G- Glioblastoma
0.0


SK-N-SH- Neuroblastoma (metastasis)
0.0


SF-295- Glioblastoma
0.0


Cerebellum
0.8


Cerebellum
0.2


NCI-H292- Mucoepidermoid lung carcinoma
0.0


DMS-114- Small cell lung cancer
0.1


DMS-79- Small cell lung cancer
9.9


NCI-H146- Small cell lung cancer
1.2


NCI-H526- Small cell lung cancer
0.0


NCI-N417- Small cell lung cancer
0.0


NCI-H82- Small cell lung cancer
28.5


NCI-H157- Squamous cell lung cancer (metastasis)
0.0


NCI-H1155- Large cell lung cancer
3.4


NCI-H1299- Large cell lung cancer
0.0


NCI-H727- Lung carcinoid
0.4


NCI-UMC-11- Lung carcinoid
10.4


LX-1- Small cell lung cancer
27.9


Colo-205- Colon cancer
3.0


KM12- Colon cancer
3.3


KM20L2- Colon cancer
1.2


NCI-H716- Colon cancer
0.0


SW-48- Colon adenocarcinoma
7.3


SW1116- Colon adenocarcinoma
3.7


LS 174T- Colon adenocarcinoma
0.2


SW-948- Colon adenocarcinoma
0.0


SW-480- Colon adenocarcinoma
3.1


NCI-SNU-5- Gastric carcinoma
0.0


KATO III- Gastric carcinoma
0.0


NCI-SNU-16- Gastric carcinoma
0.0


NCI-SNU-1- Gastric carcinoma
26.6


RF-1- Gastric adenocarcinoma
0.0


RF-48- Gastric adenocarcinoma
0.1


MKN-45- Gastric carcinoma
0.0


NCI-N87- Gastric carcinoma
0.0


OVCAR-5- Ovarian carcinoma
0.0


RL95-2- Uterine carcinoma
0.0


HelaS3- Cervical adenocarcinoma
0.0


Ca Ski- Cervical epidermoid carcinoma (metastasis)
0.0


ES-2- Ovarian clear cell carcinoma
0.0


Ramos- Stimulated with PMA/ionomycin 6 h
0.0


Ramos- Stimulated with PMA/ionomycin 14 h
0.0


MEG-01- Chronic myelogenous leukemia (megokaryoblast)
0.0


Raji- Burkitt's lymphoma
0.0


Daudi- Burkitt's lymphoma
0.0


U266- B-cell plasmacytoma
0.0


CA46- Burkitt's lymphoma
0.0


RL- non-Hodgkin's B-cell lymphoma
0.0


JM1- pre-B-cell lymphoma
0.0


Jurkat- T cell leukemia
0.0


TF-1- Erythroleukemia
0.0


HUT 78- T-cell lymphoma
0.0


U937- Histiocytic lymphoma
0.0


KU-812- Myelogenous leukemia
0.0


769-P- Clear cell renal carcinoma
0.0


Caki-2- Clear cell renal carcinoma
0.0


SW 839- Clear cell renal carcinoma
0.0


Rhabdoid kidney tumor
0.0


Hs766T- Pancreatic carcinoma (LN metastasis)
0.0


CAPAN-1- Pancreatic adenocarcinoma (liver metastasis)
9.0


SU86.86- Pancreatic carcinoma (liver metastasis)
17.6


BxPC-3- Pancreatic adenocarcinoma
0.0


HPAC- Pancreatic adenocarcinoma
1.7


MIA PaCa-2- Pancreatic carcinoma
0.0


CFPAC-1- Pancreatic ductal adenocarcinoma
58.2


PANC-1- Pancreatic epithelioid ductal carcinoma
0.0


T24- Bladder carcinoma (transitional cell)
0.0


5637- Bladder carcinoma
0.0


HT-1197- Bladder carcinoma
0.0


UM-UC-3- Bladder carcinoma (transitional cell)
0.0


A204- Rhabdomyosarcoma
0.0


HT-1080- Fibrosarcoma
0.0


MG-63- Osteosarcoma
0.0


SK-LMS-1- Leiomyosarcoma (vulva)
0.0


SJRH30- Rhabdomyosarcoma (met to bone marrow)
2.5


A431- Epidermoid carcinoma
0.0


WM266-4- Melanoma
0.2


DU 145- Prostate carcinoma (brain metastasis)
0.0


MDA-MB-468- Breast adenocarcinoma
11.2


SCC-4- Squamous cell carcinoma of tongue
0.0


SCC-9- Squamous cell carcinoma of tongue
0.0


SCC-15- Squamous cell carcinoma of tongue
0.0


CAL 27- Squamous cell carcinoma of tongue
0.0







Column A - Rel. Exp. (%) Ag819, Run 172133330














TABLE ACF










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
0.0



97476_Patient-07sk_skeletal muscle
0.5



97477_Patient-07ut_uterus
0.0



97478_Patient-07pl_placenta
12.4



99167_Bayer Patient 1
7.9



97482_Patient-08ut_uterus
0.4



97483_Patient-08pl_placenta
20.9



97486_Patient-09sk_skeletal muscle
0.0



97487_Patient-09ut_uterus
0.0



97488_Patient-09pl_placenta
11.0



97492_Patient-10ut_uterus
8.1



97493_Patient-10pl_placenta
47.0



97495_Patient-11go_adipose
0.0



97496_Patient-11sk_skeletal muscle
1.2



97497_Patient-11ut_uterus
1.5



97498_Patient-11pl_placenta
5.4



97500_Patient-12go_adipose
0.0



97501_Patient-12sk_skeletal muscle
1.1



97502_Patient-12ut_uterus
0.0



97503_Patient-12pl_placenta
11.7



94721_Donor 2 U - A_Mesenchymal Stem Cells
0.0



94722_Donor 2 U - B_Mesenchymal Stem Cells
0.0



94723_Donor 2 U - C_Mesenchymal Stem Cells
0.0



94709_Donor 2 AM - A_adipose
0.0



94710_Donor 2 AM - B_adipose
0.0



94711_Donor 2 AM - C_adipose
0.0



94712_Donor 2 AD - A_adipose
0.0



94713_Donor 2 AD - B_adipose
0.3



94714_Donor 2 AD - C_adipose
0.0



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
0.0



94730_Donor 3 AM - A_adipose
0.0



94731_Donor 3 AM - B_adipose
0.0



94732_Donor 3 AM - C_adipose
0.0



94733_Donor 3 AD - A_adipose
0.0



94734_Donor 3 AD - B_adipose
0.0



94735_Donor 3 AD - C_adipose
0.0



77138_Liver_HepG2untreated
100.0



73556_Heart_Cardiac stromal cells (primary)
0.0



81735_Small Intestine
38.2



72409_Kidney_Proximal Convoluted Tubule
4.9



82685_Small intestine_Duodenum
1.0



90650_Adrenal_Adrenocortical adenoma
0.0



72410_Kidney_HRCE
39.5



72411_Kidney_HRE
97.9



73139_Uterus_Uterine smooth muscle cells
0.0









Column A - Rel. Exp. (%) Ag819, Run 229431867







HASS Panel v1.0 Summary: Ag819 Highest expression of this gene was detected in the pancreatic cancer CaPaN cell lines subjected to acidosis (CT=27.9). Moderate levels of expression of this gene were seen in control and in CaPaN cells subjected to environmental stress. The expression of this gene was not significantly changed by oxygen deprivation, acidic or a serum starved environment in these cells. However, moderate to low expression was also shown in astrocytes, Renal Proximal Tubule Epithelial cell A2, ⅘ glioma and 2/4 medulloblastoma tissue samples in this panel. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drugs is useful in the treatment of pancreatic and brain cancers.


Panel 1.2 Summary: Ag819 The expression of this gene was highest in a colon cancer cell line (CaCo-2)(CTs=25). In addition, significant expression of this gene wais also seen in colon, ovarian, lung, pancreatic and a renal cancer cell lines. The expression of this gene is useful as a marker to detect the presence of these cancers and therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drug is useful in the treatment of these cancers.


Among tissues with metabolic function, this gene had moderate levels of expression in pancreas, adrenal gland, thyroid, pituitary gland, skeletal muscle, and adult and fetal liver. Modulation of this gene and/or expressed protein is useful in the treatment of diseases of the metabolic-endocrine axis, including obesity and Types 1 and 2 diabetes. In addition, this gene was differentially expressed in adult (CT value=28) vs fetal heart (CT value=32-33), and targeting this gene or gene product with small molecule, antibody, or protein therapeutics is of use in the treatment of heart related disorders.


This gene also showed moderate to low expression in all CNS regions examined, with highest expression in the fetal brain (greater than 10-fold) demonstrating a role in neurodevelopment. This gene codes for a Prominin like protein. Prominin plays a role in the formation of lipid membrane protrusions, their lipid content and membrane to membrane interactions; all critical for synapse formation. The expression of this gene in the developing brain shows a role in synaptogenesis for this molecule. Compensatory synaptogenesis has been shown to occur in the adult brain, especially in response to brain injury or neuronal loss. Therapeutic modulation of this gene and/or expressed protein is useful in the treatment of neurological disorders including stroke, head trauma, spinal cord trauma, Alzheimer's, Parkinson's or Huntington's diseases, multiple sclerosis, or ALS.


Panel 2D Summary: Ag819 Highest expression of this gene was detected in samples derived from an ovarian cancer and a lung cancer (CTs=26). Significant expression of this gene was seen in a number of cancer samples including a kidney cancer, two colon cancers and two gastric cancers. The expression of this gene is useful as a marker to detect the presence of ovarian or lung cancers. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drugs is of benefit in the treatment of ovarian, lung, kidney, colon or gastric cancers.


Panel 3D Summary: Ag819 The expression of this gene was highest in a sample derived from a brain cancer (meduloblastoma) cell line (D283 cells)(CT=28.3). Significant expression of this gene was also seen in several cell lines derived from lung, gastric and pancreatic cancers. Expression of this gene is useful as a marker to detect these cancers and therapeutic modulation of this gene, expressed protein, and/or use of antibodies or small molecule drugs targeting this gene or gene product is useful in the treatment of lung, brain, pancreatic or gastric cancers.


Panel 5 Islet Summary: Ag819 Highest expression of this gene was detected in liver cancer HepG2 cell line (CT=31). Low levels of expression of this gene are also seen in islets cells (Bayer patient 1), small intestine, kidney and placenta. Therefore, modulation of this gene and/or expressed protein is useful in treatment of metabolic diseases including obesity and type II diabetes.


AD. CG55193-04: PV1 Protein


Expression of gene CG55193-04 was assessed using the primer-probe sets Ag1537 and Ag4932, described in Tables ADA and ADB. Results of the RTQ-PCR runs are shown in Tables ADD, ADE, ADF, ADG, ADH and ADI.

TABLE ADAProbe Name Ag1537SEQStartIDPrimersSequencesLengthPositionNoForward5′-aaggagctggaagagaagaaga-3′2210871405ProbeTET-5′-atcagaaactcagccctggacacctg-3′-2611411406TAMRAReverse5′-gctgcgacttggtcttgat-3′1911681407









TABLE ADB










Probe Name Ag4932
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-aatgcagagatcaattcaagga-3′
22
425
1408






Probe
TET-5′-aacaagagctgcgatgccttgctctt-3′-
26
451
1409



TAMRA





Reverse
5′-tcttcaccttctgattcagcat-3′
22
478
1410
















TABLE ADC










Ardais Kidney 1.0










Tissue Name
A














Kidney cancer(10A8)
2.3



Kidney NAT(10A9)
6.9



Kidney cancer(10AA)
100.0



Kidney NAT(10AB)
7.4



Kidney cancer(10AC)
25.7



Kidney NAT(10AD)
5.3



Kidney cancer(10B6)
8.7



Kidney NAT(10B7)
4.5



Kidney cancer(10B8)
2.2



Kidney NAT(10B9)
10.6



Kidney cancer(10BC)
2.0



Kidney NAT(10BD)
20.2



Kidney cancer(10BE)
23.5



Kidney NAT(10BF)
9.7



Kidney cancer(10C2)
1.1



Kidney NAT(10C3)
9.1



Kidney cancer(10C4)
14.4



Kidney NAT(10C5)
9.4



Kidney cancer(10B4)
2.2



Kidney cancer(10C8)
14.5



Kidney cancer(10D0)
60.3



Kidney cancer(10C0)
1.2



Kidney cancer(10C6)
3.3



Kidney cancer(10C9)
1.5



Kidney cancer(10D1)
85.3



Kidney cancer(10CA)
0.8



Kidney cancer(10D2)
55.1



Kidney cancer(10CB)
6.4



Kidney cancer(10D4)
22.1



Kidney cancer(10CD)
73.2



Kidney cancer(10D5)
7.4



Kidney cancer(10CE)
63.3



Kidney cancer(10D6)
38.2



Kidney cancer(10CF)
22.8



Kidney cancer(10D8)
25.7



Kidney cancer(10CC)
28.1



Kidney cancer(10D3)
12.0



Kidney NAT(10D9)
7.4



Kidney NAT(10DB)
7.2



Kidney NAT(10DC)
10.8



Kidney NAT(10DD)
12.9



Kidney NAT(10DE)
20.7



Kidney NAT(10B1)
7.5



Kidney NAT(10DA)
7.5









Column A - Rel. Exp. (%) Ag1537, Run 385860665














TABLE ADD










Ardais Panel v.1.0










Tissue Name
A














136799_Lung cancer(362)
23.8



136800_Lung NAT(363)
15.6



136813_Lung cancer(372)
45.4



136814_Lung NAT(373)
14.4



136815_Lung cancer(374)
39.2



136816_Lung NAT(375)
100.0



136791_Lung cancer(35A)
22.5



136795_Lung cancer(35E)
35.4



136797_Lung cancer(360)
22.4



136794_lung NAT(35D)
14.3



136818_Lung NAT(377)
33.0



136787_lung cancer(356)
8.1



136788_lung NAT(357)
52.5



136806_Lung cancer(36B)
35.6



136807_Lung NAT(36C)
18.8



136789_lung cancer(358)
65.1



136802_Lung cancer(365)
49.3



136803_Lung cancer(368)
24.5



136804_Lung cancer(369)
38.2



136811_Lung cancer(370)
14.9



136810_Lung NAT(36F)
31.4









Column A - Rel. Exp. (%) Ag1537, Run 267680189














TABLE ADE










General_screening_panel_v1.5










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
10.7



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
14.7



Placenta
42.6



Uterus Pool
53.6



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.2



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.1



Ovary
11.1



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
62.4



Trachea
47.3



Lung
4.3



Fetal Lung
17.2



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.0



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
1.2



Fetal Liver
44.1



Liver ca. HepG2
0.0



Kidney Pool
55.1



Fetal Kidney
73.2



Renal ca. 786-0
0.0



Renal ca. A498
0.1



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
86.5



Gastric ca. (liver met.) NCI-N87
4.5



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
51.1



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
89.5



Small Intestine Pool
11.7



Stomach Pool
37.9



Bone Marrow Pool
46.0



Fetal Heart
15.1



Heart Pool
22.2



Lymph Node Pool
66.9



Fetal Skeletal Muscle
23.2



Skeletal Muscle Pool
32.5



Spleen Pool
100.0



Thymus Pool
30.6



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.1



CNS cancer (neuro; met) SK-N-AS
0.1



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.7



Brain (Amygdala) Pool
1.0



Brain (cerebellum)
2.0



Brain (fetal)
2.6



Brain (Hippocampus) Pool
2.1



Cerebral Cortex Pool
1.3



Brain (Substantia nigra) Pool
1.5



Brain (Thalamus) Pool
2.5



Brain (whole)
3.8



Spinal Cord Pool
1.9



Adrenal Gland
55.1



Pituitary gland Pool
10.3



Salivary Gland
20.7



Thyroid (female)
70.7



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
53.6









Column A - Rel. Exp. (%) Ag4932, Run 228843451














TABLE ADF










Oncology_cell_line_screening_panel_v3.2








Tissue Name
A











94905_Daoy_Medulloblastoma/Cerebellum_sscDNA
0.0


94906_TE671_Medulloblastom/Cerebellum_sscDNA
0.0


94907_D283
0.0


Med_Medulloblastoma/Cerebellum_sscDNA


94908_PFSK-1_Primitive
0.5


Neuroectodermal/Cerebellum_sscDNA


94909_XF-498_CNS_sscDNA
0.0


94910_SNB-78_CNS/glioma_sscDNA
0.0


94911_SF-268_CNS/glioblastoma_sscDNA
0.0


94912_T98G_Glioblastoma_sscDNA
0.0


96776_SK-N-SH_Neuroblastoma (metastasis)_sscDNA
0.0


94913_SF-295_CNS/glioblastoma_sscDNA
0.0


132565_NT2 pool_sscDNA
0.1


94914_Cerebellum_sscDNA
0.2


96777_Cerebellum_sscDNA
0.3


94916_NCI-H292_Mucoepidermoid lung carcinoma_sscDNA
0.0


94917_DMS-114_Small cell lung cancer_sscDNA
0.0


94918_DMS-79_Small cell lung
0.0


cancer/neuroendocrine_sscDNA


94919_NCI-H146_Small cell
0.0


lung cancer/neuroendocrine_sscDNA


94920_NCI-H526_Small cell
0.0


lung cancer/neuroendocrine_sscDNA


94921_NCI-N417_Small cell
0.0


lung cancer/neuroendocrine_sscDNA


94923_NCI-H82_Small cell lung
0.0


cancer/neuroendocrine_sscDNA


94924_NCI-H157_Squamous cell lung cancer
0.0


(metastasis)_sscDNA


94925_NCI-H1155_Large cell lung
0.0


cancer/neuroendocrine_sscDNA


94926_NCI-H1299_Large cell lung
0.0


cancer/neuroendocrine_sscDNA


94927_NCI-H727_Lung carcinoid_sscDNA
0.0


94928_NCI-UMC-11_Lung carcinoid_sscDNA
0.0


94929_LX-1_Small cell lung cancer_sscDNA
0.0


94930_Colo-205_Colon cancer_sscDNA
0.0


94931_KM12_Colon cancer_sscDNA
0.0


94932_KM20L2_Colon cancer_sscDNA
0.0


94933_NCI-H716_Colon cancer_sscDNA
0.0


94935_SW-48_Colon adenocarcinoma_sscDNA
0.0


94936_SW1116_Colon
0.0


adenocarcinoma_sscDNA


94937_LS 174T_Colon adenocarcinoma_sscDNA
0.0


94938_SW-948_Colon adenocarcinoma_sscDNA
0.0


94939_SW-480_Colon adenocarcinoma_sscDNA
0.0


94940_NCI-SNU-5_Gastric carcinoma_sscDNA
0.0


112197_KATO III_Stomach_sscDNA
0.0


94943_NCI-SNU-16_Gastric carcinoma_sscDNA
0.0


94944_NCI-SNU-1_Gastric carcinoma_sscDNA
0.0


94946_RF-1_Gastric adenocarcinoma_sscDNA
0.0


94947_RF-48_Gastric adenocarcinoma_sscDNA
0.1


96778_MKN-45_Gastric carcinoma_sscDNA
0.0


94949_NCI-N87_Gastric carcinoma_sscDNA
0.0


94951_OVCAR-5_Ovarian carcinoma_sscDNA
0.0


94952_RL95-2_Uterine carcinoma_sscDNA
0.0


94953_HelaS3_Cervical adenocarcinoma_sscDNA
0.0


94954_Ca Ski_Cervical epidermoid carcinoma
0.2


(metastasis)_sscDNA


94955_ES-2_Ovarian clear cell carcinoma_sscDNA
0.0


94957_Ramos/6 h stim_Stimulated with PMA/ionomycin
0.0


6 h_sscDNA


94958_Ramos/14 h stim_Stimulated with PMA/ionomycin
0.0


14 h_sscDNA


94962_MEG-01_Chronic myelogenous leukemia
0.7


(megokaryoblast)_sscDNA


94963_Raji_Burkitt's lymphoma_sscDNA
0.0


94964_Daudi_Burkitt's lymphoma_sscDNA
0.8


94965_U266_B-cell plasmacytoma/myeloma_sscDNA
1.3


94968_CA46_Burkitt's lymphoma_sscDNA
0.2


94970_RL_non-Hodgkin's B-cell lymphoma_sscDNA
0.0


94972_JM1_pre-B-cell lymphoma/leukemia_sscDNA
0.0


94973_Jurkat_T cell leukemia_sscDNA
0.0


94974_TF-1_Erythroleukemia_sscDNA
100.0


94975_HUT 78_T-cell lymphoma_sscDNA
0.0


94977_U937_Histiocytic lymphoma_sscDNA
0.0


94980_KU-812_Myelogenous leukemia_sscDNA
28.9


94981_769-P_Clear cell renal carcinoma_sscDNA
0.1


94983_Caki-2_Clear cell renal carcinoma_sscDNA
0.0


94984_SW 839_Clear cell renal carcinoma_sscDNA
0.0


94986_G401_Wilms' tumor_sscDNA
0.0


126768_293 cells_sscDNA
0.0


94987_Hs766T_Pancreatic carcinoma (LN
0.6


metastasis)_sscDNA


94988_CAPAN-1_Pancreatic adenocarcinoma (liver
0.0


metastasis)_sscDNA


94989_SU86.86_Pancreatic carcinoma (liver
0.0


metastasis)_sscDNA


94990_BxPC-3_Pancreatic adenocarcinoma_sscDNA
0.0


94991_HPAC_Pancreatic adenocarcinoma_sscDNA
0.0


94992_MIA PaCa-2_Pancreatic carcinoma_sscDNA
0.0


94993_CFPAC-1_Pancreatic ductal
0.1


adenocarcinoma_sscDNA


94994_PANC-1_Pancreatic epithelioid ductal
0.0


carcinoma_sscDNA


94996_T24_Bladder carcinoma (transitional
0.1


cell)_sscDNA


94997_5637_Bladder carcinoma_sscDNA
0.0


94998_HT-1197_Bladder carcinoma_sscDNA
0.0


94999_UM-UC-3_Bladder carcinoma (transitional
0.0


cell)_sscDNA


95000_A204_Rhabdomyosarcoma_sscDNA
0.0


95001_HT-1080_Fibrosarcoma_sscDNA
0.0


95002_MG-63_Osteosarcoma (bone)_sscDNA
0.0


95003_SK-LMS-1_Leiomyosarcoma (vulva)_sscDNA
0.2


95004_SJRH30_Rhabdomyosarcoma (met to bone
0.0


marrow)_sscDNA


95005_A431_Epidermoid carcinoma_sscDNA
0.0


95007_WM266-4_Melanoma_sscDNA
0.0


112195_DU 145_Prostate_sscDNA
0.0


95012_MDA-MB-468_Breast adenocarcinoma_sscDNA
0.0


112196_SSC-4_Tongue_sscDNA
0.0


112194_SSC-9_Tongue_sscDNA
0.0


112191_SSC-15_Tongue_sscDNA
0.0


95017_CAL 27_Squamous cell carcinoma of
0.0


tongue_sscDNA







Column A - Rel. Exp. (%) Ag1537, Run 267177741














TABLE ADG










Panel 2D










Tissue Name
A














Normal Colon
12.3



CC Well to Mod Diff (ODO3866)
10.7



CC Margin (ODO3866)
12.2



CC Gr.2 rectosigmoid (ODO3868)
3.2



CC Margin (ODO3868)
0.8



CC Mod Diff (ODO3920)
3.4



CC Margin (ODO3920)
2.2



CC Gr.2 ascend colon (ODO3921)
13.4



CC Margin (ODO3921)
5.8



CC from Partial Hepatectomy (ODO4309) Mets
9.6



Liver Margin (ODO4309)
0.6



Colon mets to lung (OD04451-01)
5.5



Lung Margin (OD04451-02)
0.8



Normal Prostate 6546-1
14.1



Prostate Cancer (OD04410)
8.8



Prostate Margin (OD04410)
6.9



Prostate Cancer (OD04720-01)
3.1



Prostate Margin (OD04720-02)
10.3



Normal Lung 061010
11.8



Lung Met to Muscle (ODO4286)
6.4



Muscle Margin (ODO4286)
9.9



Lung Malignant Cancer (OD03126)
19.3



Lung Margin (OD03126)
3.3



Lung Cancer (OD04404)
5.2



Lung Margin (OD04404)
25.3



Lung Cancer (OD04565)
3.4



Lung Margin (OD04565)
3.1



Lung Cancer (OD04237-01)
11.0



Lung Margin (OD04237-02)
18.2



Ocular Mel Met to Liver (ODO4310)
0.7



Liver Margin (ODO4310)
1.7



Melanoma Mets to Lung (OD04321)
3.9



Lung Margin (OD04321)
3.7



Normal Kidney
40.6



Kidney Ca, Nuclear grade 2 (OD04338)
5.7



Kidney Margin (OD04338)
11.1



Kidney Ca Nuclear grade 1/2 (OD04339)
2.5



Kidney Margin (OD04339)
17.6



Kidney Ca, Clear cell type (OD04340)
100.0



Kidney Margin (OD04340)
22.7



Kidney Ca, Nuclear grade 3 (OD04348)
55.1



Kidney Margin (OD04348)
19.9



Kidney Cancer (OD04622-01)
25.0



Kidney Margin (OD04622-03)
7.4



Kidney Cancer (OD04450-01)
1.3



Kidney Margin (OD04450-03)
9.2



Kidney Cancer 8120607
9.2



Kidney Margin 8120608
23.5



Kidney Cancer 8120613
21.5



Kidney Margin 8120614
12.3



Kidney Cancer 9010320
34.4



Kidney Margin 9010321
27.7



Normal Uterus
9.3



Uterus Cancer 064011
6.4



Normal Thyroid
84.1



Thyroid Cancer 064010
20.6



Thyroid Cancer A302152
15.2



Thyroid Margin A302153
21.3



Normal Breast
22.1



Breast Cancer (OD04566)
8.4



Breast Cancer (OD04590-01)
21.0



Breast Cancer Mets (OD04590-03)
27.7



Breast Cancer Metastasis (OD04655-05)
9.1



Breast Cancer 064006
10.1



Breast Cancer 1024
7.1



Breast Cancer 9100266
10.4



Breast Margin 9100265
7.4



Breast Cancer A209073
27.4



Breast Margin A209073
8.7



Normal Liver
1.1



Liver Cancer 064003
6.5



Liver Cancer 1025
0.7



Liver Cancer 1026
8.1



Liver Cancer 6004-T
1.9



Liver Tissue 6004-N
3.6



Liver Cancer 6005-T
9.3



Liver Tissue 6005-N
0.6



Normal Bladder
14.1



Bladder Cancer 1023
4.5



Bladder Cancer A302173
3.6



Bladder Cancer (OD04718-01)
7.4



Bladder Normal Adjacent (OD04718-03)
15.2



Normal Ovary
1.4



Ovarian Cancer 064008
6.5



Ovarian Cancer (OD04768-07)
1.6



Ovary Margin (OD04768-08)
9.2



Normal Stomach
13.5



Gastric Cancer 9060358
2.8



Stomach Margin 9060359
12.6



Gastric Cancer 9060395
20.6



Stomach Margin 9060394
7.5



Gastric Cancer 9060397
10.0



Stomach Margin 9060396
3.2



Gastric Cancer 064005
6.7









Column A - Rel. Exp. (%) Ag1537, Run 145017308














TABLE ADH










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.1



Secondary Th2 act
0.4



Secondary Tr1 act
0.1



Secondary Th1 rest
0.1



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
1.2



CD45RO CD4 lymphocyte act
0.2



CD8 lymphocyte act
0.1



Secondary CD8 lymphocyte rest
0.4



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.3



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.1



LAK cells IL-2
0.1



LAK cells IL-2 + IL-12
0.2



LAK cells IL-2 + IFN gamma
0.5



LAK cells IL-2 + IL-18
0.2



LAK cells PMA/ionomycin
0.2



NK Cells IL-2 rest
0.2



Two Way MLR 3 day
2.7



Two Way MLR 5 day
1.3



Two Way MLR 7 day
0.1



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.1



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.5



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.1



Dendritic cells none
0.1



Dendritic cells LPS
1.7



Dendritic cells anti-CD40
0.9



Monocytes rest
0.6



Monocytes LPS
0.1



Macrophages rest
0.0



Macrophages LPS
0.1



HUVEC none
1.9



HUVEC starved
8.4



HUVEC IL-1beta
5.6



HUVEC IFN gamma
40.6



HUVEC TNF alpha + IFN gamma
4.6



HUVEC TNF alpha + IL4
5.0



HUVEC IL-11
8.7



Lung Microvascular EC none
66.4



Lung Microvascular EC TNFalpha + IL-1beta
30.4



Microvascular Dermal EC none
43.5



Microsvasular Dermal EC TNFalpha + IL-1beta
17.0



Bronchial epithelium TNFalpha + IL1beta
0.3



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
1.1



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.2



KU-812 (Basophil) rest
27.0



KU-812 (Basophil) PMA/ionomycin
28.3



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
20.6



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.1



NCI-H292 IFN gamma
0.0



HPAEC none
1.8



HPAEC TNF alpha + IL-1 beta
1.5



Lung fibroblast none
0.4



Lung fibroblast TNF alpha + IL-1 beta
0.6



Lung fibroblast IL-4
0.2



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.4



Lung fibroblast IFN gamma
0.2



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
1.1



Dermal fibroblast IL-4
0.4



Dermal Fibroblasts rest
0.7



Neutrophils TNFa + LPS
0.4



Neutrophils rest
0.3



Colon
19.3



Lung
100.0



Thymus
48.0



Kidney
68.8









Column A - Rel. Exp. (%) Ag4932, Run 223597251














TABLE ADI










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
11.4



Colon cancer NAT 1
9.4



Colon cancer 2
4.1



Colon cancer NAT 2
4.1



Colon cancer 3
10.4



Colon cancer NAT 3
7.6



Colon malignant cancer 4
9.7



Colon normal adjacent tissue 4
3.4



Lung cancer 1
4.8



Lung NAT 1
0.7



Lung cancer 2
5.6



Lung NAT 2
0.1



Squamous cell carcinoma 3
5.4



Lung NAT 3
0.7



metastatic melanoma 1
1.5



Melanoma 2
4.0



Melanoma 3
3.8



metastatic melanoma 4
2.2



metastatic melanoma 5
4.6



Bladder cancer 1
1.0



Bladder cancer NAT 1
0.0



Bladder cancer 2
4.4



Bladder cancer NAT 2
0.6



Bladder cancer NAT 3
0.8



Bladder cancer NAT 4
4.2



Prostate adenocarcinoma 1
2.1



Prostate adenocarcinoma 2
1.2



Prostate adenocarcinoma 3
2.1



Prostate adenocarcinoma 4
6.0



Prostate cancer NAT 5
3.0



Prostate adenocarcinoma 6
1.3



Prostate adenocarcinoma 7
1.2



Prostate adenocarcinoma 8
1.2



Prostate adenocarcinoma 9
4.8



Prostate cancer NAT 10
0.6



Kidney cancer 1
90.1



Kidney NAT 1
5.0



Kidney cancer 2
60.3



Kidney NAT 2
9.8



Kidney cancer 3
30.8



Kidney NAT 3
5.4



Kidney cancer 4
100.0



Kidney NAT 4
6.7









Column A - Rel. Exp. (%) Ag1537, Run 266930996







Ardais Kidney 1.0 Summary: Ag1537 Highest expression was seen in a kidney cancer sample (CT=21.3). In addition, this gene was more highly expressed in kidney cancer than in the corresponding normal adjacent tissue. Expression of this gene is useful as a marker of these cancers. Therapeutic modulation of the expression or function of this gene or gene product is useful in the treatment of kidney cancer.


Ardais Panel v.1.0 Summary: Ag1537 Highest expression of this gene was detected in a normal lung sample (CT=26.7). High to moderate levels of expression are seen in both malignant and normal lung samples. Therapeutic modulation of the PV1 protein (PLVAP) encoded by this gene is useful in the treatment of certain subtypes of lung cancer.


General_screening13 panel_v1.5 Summary: Ag4932 Highest expression of this gene was detected in the spleen (CT=26). High levels of expression of this gene were also detected in tissues with metabolic/endocrine functions including pancreas, adipose, adrenal gland, thyroid, pituitary gland, skeletal muscle, heart, liver and the gastrointestinal tract. The PV-1-like protein is a plasma membrane protein with an extracellular domain that is an antibody target for the treatment of endocrine/metabolically related diseases, such as obesity and diabetes.


Moderate levels of expression of this gene were also seen in all regions of the central nervous system examined, including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. Therapeutic modulation of this gene or gene product is useful in the treatment of central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


In addition, this gene also showed high expression in colon cancer tissue, with moderate levels of expression in a gastric NCI-N87 cell line. Therapeutic modulation of this gene is useful in the treatment of colon and gastric cancers.


Oncology13 cell13 line_screening13 panel_v3.2 Summary: Ag1537 Highest expression of this gene was detected in TF-1 erythroleukemia cells (CT=28.6). Moderate levels of expression of this gene are restricted to erythroleukemia and myelogenous leukemia. Expression of this gene is useful as a marker to distinguish these leukemia samples from other samples in the panel and also, as marker to detect the presence of these leukemia. Therapeutic modulation of this gene or its protein product is useful in the treatment of erythroleukemia and myelogenous leukemia.


Panel 2D Summary: Ag1537 Expression of this gene was highest in a kidney cancer (OD04340) sample (CT=25). This gene was widely expressed across this panel with high to moderate expression in both normal and adjacent cancer tissue. This gene was more highly expressed in kidney cancer tissue than in adjacent normal tissue, consistent with expression pattern seen in panel 2.4. This gene is useful as a marker to distinguish kidney cancers from normal kidney tissue. Therapeutic modulation of this gene or its protein product, through the use of small molecule drugs or antibodies, is of benefit in the treatment of kidney cancer.


Panel 4.1D Summary: Ag4932 Highest expression of this gene was detected in the lung (CT=28.5). Moderate levels of expression of this gene were also seen in endothelial cells, basophils and normal tissues represented by colon, thymus and kidney. This gene codes for a variant of PV-1, a component of the endothelial fenestral and stomatal diaphragms. Antibodies raised against the PV-1 encoded by this gene are useful in preventing transendothelial trafficking of inflammatory cells to different tissues sites and as a treatment of inflammatory diseases including delayed type hypersensitivity, asthma, emphysema, rheumatoid arthritis and IBD.


Moderate levels of expression of this gene were also seen in liver cirrhosis samples. Therefore, targeting this gene or its protein product with protein therapeutics, antibodies or small molecule therapeutics is useful as a treatment to reduce or inhibit fibrosis that occurs in liver cirrhosis.


general oncology screening panel_v—2.4 Summary: Ag1537/Ag760 Highest expression of this gene was seen in a kidney cancer sample (CTs=22.6-25). Significant expression of this gene was seen in melanoma, colon, lung, prostate, bladder and kidney cancer as well as normal tissue samples. Expression of this gene was higher in kidney cancer as compared to corresponding normal control samples. Expression of this gene is useful as a marker to distinguish kidney cancer from normal tissue and also as a marker to detect kidney cancer. Therapeutic modulation of this gene or its protein product through the use of antibodies, protein therapeutics or small molecule drug is useful in the treatment of melanoma, kidney, colon, lung and prostate cancers.


AE. CG5525607: Apolipoprotein E Receptor 2


Expression of gene CG55256-07 was assessed using the primer-probe set Ag6829, described in Table AEA. Results of the RTQ-PCR runs are shown in Tables AEB and AEC.

TABLE AEAProbe Name Ag6829StartSEQ IDPrimersSequencesLengthPositionNoForward5′-aggacatgctgtggatgaag-3′2039871411ProbeTET-5′-tcacatctcttatttctcttttcccctt-3′-2840311412TAMRAReverse5′-ccccacataaaggaaatggt-3′2040661413









TABLE AEB










General_screening_panel_v1.7










Tissue Name
A














Adipose
1.5



HUVEC
3.6



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
4.5



Melanoma (met) SK-MEL-5
5.7



Testis
4.8



Prostate ca. (bone met) PC-3
0.0



Prostate ca. DU145
5.0



Prostate pool
0.1



Uterus pool
0.0



Ovarian ca. OVCAR-3
1.9



Ovarian ca. (ascites) SK-OV-3
0.1



Ovarian ca. OVCAR-4
13.3



Ovarian ca. OVCAR-5
13.2



Ovarian ca. IGROV-1
29.9



Ovarian ca. OVCAR-8
13.9



Ovary
0.4



Breast ca. MCF-7
3.2



Breast ca. MDA-MB-231
12.7



Breast ca. BT-549
3.2



Breast ca. T47D
1.5



Breast pool
0.0



Trachea
1.4



Lung
1.3



Fetal Lung
1.4



Lung ca. NCI-N417
2.2



Lung ca. LX-1
1.9



Lung ca. NCI-H146
3.8



Lung ca. SHP-77
40.9



Lung ca. NCI-H23
8.1



Lung ca. NCI-H460
46.3



Lung ca. HOP-62
15.3



Lung ca. NCI-H522
9.2



Lung ca. DMS-114
1.4



Liver
0.0



Fetal Liver
0.1



Kidney pool
0.8



Fetal Kidney
0.1



Renal ca. 786-0
15.6



Renal ca. A498
1.0



Renal ca. ACHN
4.2



Renal ca. UO-31
1.1



Renal ca. TK-10
7.6



Bladder
0.2



Gastric ca. (liver met.) NCI-N87
1.3



Stomach
0.0



Colon ca. SW-948
2.6



Colon ca. SW480
1.1



Colon ca. (SW480 met) SW620
14.4



Colon ca. HT29
100.0



Colon ca. HCT-116
30.6



Colon cancer tissue
0.0



Colon ca. SW1116
7.6



Colon ca. Colo-205
4.4



Colon ca. SW-48
1.8



Colon
0.1



Small Intestine
0.0



Fetal Heart
0.0



Heart
0.0



Lymph Node pool 1
0.0



Lymph Node pool 2
3.3



Fetal Skeletal Muscle
0.1



Skeletal Muscle pool
0.0



Skeletal Muscle
0.0



Spleen
0.2



Thymus
0.1



CNS cancer (glio/astro) SF-268
3.0



CNS cancer (glio/astro) T98G
5.7



CNS cancer (neuro; met) SK-N-AS
0.6



CNS cancer (astro) SF-539
10.5



CNS cancer (astro) SNB-75
9.3



CNS cancer (glio) SNB-19
23.0



CNS cancer (glio) SF-295
3.1



Brain (Amygdala)
3.4



Brain (Cerebellum)
4.5



Brain (Fetal)
24.0



Brain (Hippocampus)
1.9



Cerebral Cortex pool
2.5



Brain (Substantia nigra)
1.2



Brain (Thalamus)
3.3



Brain (Whole)
21.9



Spinal Cord
0.5



Adrenal Gland
0.3



Pituitary Gland
2.4



Salivary Gland
0.1



Thyroid
13.9



Pancreatic ca. PANC-1
1.2



Pancreas pool
0.1









Column A - Rel. Exp. (%) Ag6829, Run 318350033














TABLE AEC










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
0.0



97476_Patient-07sk_skeletal muscle
0.0



97477_Patient-07ut_uterus
0.0



97478_Patient-07pl_placenta
21.2



99167_Bayer Patient 1
2.4



97482_Patient-08ut_uterus
0.0



97483_Patient-08pl_placenta
10.7



97486_Patient-09sk_skeletal muscle
0.0



97487_Patient-09ut_uterus
0.0



97488_Patient-09pl_placenta
12.2



97492_Patient-10ut_uterus
0.0



97493_Patient-10pl_placenta
63.7



97495_Patient-11go_adipose
0.0



97496_Patient-11sk_skeletal muscle
0.0



97497_Patient-11ut_uterus
4.3



97498_Patient-11pl_placenta
15.9



97500_Patient-12go_adipose
0.0



97501_Patient-12sk_skeletal muscle
2.9



97502_Patient-12ut_uterus
5.4



97503_Patient-12pl_placenta
62.4



94721_Donor 2 U - A_Mesenchymal Stem Cells
7.4



94722_Donor 2 U - B_Mesenchymal Stem Cells
5.9



94723_Donor 2 U - C_Mesenchymal Stem Cells
6.7



94709_Donor 2 AM - A_adipose
11.9



94710_Donor 2 AM - B_adipose
5.0



94711_Donor 2 AM - C_adipose
4.5



94712_Donor 2 AD - A_adipose
7.5



94713_Donor 2 AD - B_adipose
17.6



94714_Donor 2 AD - C_adipose
8.4



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
0.0



94730_Donor 3 AM - A_adipose
19.8



94731_Donor 3 AM - B_adipose
37.9



94732_Donor 3 AM - C_adipose
29.9



94733_Donor 3 AD - A_adipose
32.5



94734_Donor 3 AD - B_adipose
17.2



94735_Donor 3 AD - C_adipose
18.9



77138_Liver_HepG2untreated
100.0



73556_Heart_Cardiac stromal cells (primary)
4.9



81735_Small Intestine
2.6



72409_Kidney_Proximal Convoluted Tubule
29.5



82685_Small intestine_Duodenum
0.0



90650_Adrenal_Adrenocortical adenoma
0.0



72410_Kidney_HRCE
67.8



72411_Kidney_HRE
37.6



73139_Uterus_Uterine smooth muscle cells
15.7









Column A - Rel. Exp. (%) Ag6829, Run 279524965







General_screening13 panel13 v1.7 Summary: Ag6829 Highest expression was seen in a colon cancer cell line (CT=28). Prominent levels of expression were seen in clusters of cell lines derived from brain, colon, lung and ovarian cancer cell lines. Targeting this gene or its protein product is useful in the treatment of these cancers.


Panel 5 Islet Summary: Ag6829 Low but significant levels of expression were seen in a liver cell line (CT=34).


AF. CG5525610: Apolipoprotein E Receptor 2


Expression of full-length physical clone CG55256-10 was assessed using the primer-probe set Ag7103, described in Table AFA. Results of the RTQ-PCR runs are shown in Table AFB.

TABLE AFAProbe Name Ag7103StartSEQ IDPrimersSequencesLengthPositionNoForward5′-cccagcttctctgcacca-3′187781414ProbeTET-5′-cctcggactcatcggagccatca-3′-238181415TAMRAReverse5′-cggcgaggaggagtgtc-3′178421416









TABLE AFB










General_screening_panel_v1.7










Tissue Name
A














Adipose
1.2



HUVEC
4.6



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
6.3



Melanoma (met) SK-MEL-5
7.8



Testis
9.2



Prostate ca. (bone met) PC-3
0.0



Prostate ca. DU145
13.7



Prostate pool
0.1



Uterus pool
0.1



Ovarian ca. OVCAR-3
0.9



Ovarian ca. (ascites) SK-OV-3
0.7



Ovarian ca. OVCAR-4
11.0



Ovarian ca. OVCAR-5
10.4



Ovarian ca. IGROV-1
19.6



Ovarian ca. OVCAR-8
8.6



Ovary
0.5



Breast ca. MCF-7
6.0



Breast ca. MDA-MB-231
12.2



Breast ca. BT-549
4.9



Breast ca. T47D
3.6



Breast pool
0.1



Trachea
1.3



Lung
1.0



Fetal Lung
1.3



Lung ca. NCI-N417
7.2



Lung ca. LX-1
6.7



Lung ca. NCI-H146
5.9



Lung ca. SHP-77
18.7



Lung ca. NCI-H23
7.9



Lung ca. NCI-H460
26.4



Lung ca. HOP-62
9.9



Lung ca. NCI-H522
7.1



Lung ca. DMS-114
2.5



Liver
0.0



Fetal Liver
0.2



Kidney pool
0.5



Fetal Kidney
0.3



Renal ca. 786-0
11.0



Renal ca. A498
3.2



Renal ca. ACHN
4.3



Renal ca. UO-31
1.7



Renal ca. TK-10
9.9



Bladder
0.5



Gastric ca. (liver met.) NCI-N87
2.6



Stomach
0.1



Colon ca. SW-948
2.7



Colon ca. SW480
4.9



Colon ca. (SW480 met) SW620
15.5



Colon ca. HT29
64.6



Colon ca. HCT-116
24.0



Colon cancer tissue
0.2



Colon ca. SW1116
19.1



Colon ca. Colo-205
8.2



Colon ca. SW-48
3.6



Colon
0.3



Small Intestine
0.0



Fetal Heart
0.1



Heart
0.1



Lymph Node pool 1
0.1



Lymph Node pool 2
2.2



Fetal Skeletal Muscle
0.3



Skeletal Muscle pool
0.0



Skeletal Muscle
0.1



Spleen
0.3



Thymus
0.5



CNS cancer (glio/astro) SF-268
5.3



CNS cancer (glio/astro) T98G
8.4



CNS cancer (neuro; met) SK-N-AS
100.0



CNS cancer (astro) SF-539
9.7



CNS cancer (astro) SNB-75
10.2



CNS cancer (glio) SNB-19
18.4



CNS cancer (glio) SF-295
6.8



Brain (Amygdala)
5.0



Brain (Cerebellum)
11.3



Brain (Fetal)
27.5



Brain (Hippocampus)
3.6



Cerebral Cortex pool
3.3



Brain (Substantia nigra)
3.0



Brain (Thalamus)
3.6



Brain (Whole)
22.5



Spinal Cord
0.8



Adrenal Gland
0.7



Pituitary Gland
4.3



Salivary Gland
0.1



Thyroid
11.9



Pancreatic ca. PANC-1
3.1



Pancreas pool
0.2









Column A - Rel. Exp. (%) Ag7103, Run 318037113







General_screening13 panel_v1.7 Summary: Ag7103 Highest expression was seen in a brain cancer cell line (CT=21), with high levels of expression seen in all the cancer cell line samples on this panel. This gene or gene product is involved in cell growth and/or proliferation. Targeting this gene or gene product is useful in the treatment of cancers, including breast, brain, ovarian, colon, renal, brain, prostate and melanoma cancers.


AG. CG5577601: Mechanical Stress Induced Protein


Expression of gene CG55776-01 was assessed using the primer-probe sets Ag1489 and Ag4335, described in Tables AGA and AGB. Results of the RTQ-PCR runs are shown in Tables AGC, AGD, AGE, AGF, AGG and AGH.

TABLE AGAProbe Name Ag1489StartSEQ IDPrimersSequencesLengthPositionNoForward5′-cacagcattgaattgctctgt-3′2170491417ProbeTET-5′-tctggattttaccaaatggcacacga-3′-2670961418TAMRAReverse5′-gataactttgtggtccattgga-3′2271251419









TABLE AGB










Probe Name Ag4335















Start
SEQ ID



Primers
Sequences
Length
Position
No





Forward
5′-cacctgaagaacccacagatt-3′
21
2593
1420






Probe
TET-5′-tgctattaaaactacagccatgtcaaaga-3′-
29
2627
1421



TAMRA





Reverse
5′-gcttgacatggttgggtttat-3′
21
2658
1422
















TABLE AGC










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
1.8



110980 COPD-F
12.1



110968 COPD-M
2.7



110977 COPD-M
0.0



110989 Emphysema-F
4.9



110992 Emphysema-F
5.7



110993 Emphysema-F
3.3



110994 Emphysema-F
1.7



110995 Emphysema-F
5.2



110996 Emphysema-F
3.1



110997 Asthma-M
0.5



111001 Asthma-F
2.2



111002 Asthma-F
2.3



111003 Atopic Asthma-F
2.3



111004 Atopic Asthma-F
6.1



111005 Atopic Asthma-F
2.6



111006 Atopic Asthma-F
0.4



111417 Allergy-M
3.4



112347 Allergy-M
0.1



112349 Normal Lung-F
0.2



112357 Normal Lung-F
100.0



112354 Normal Lung-M
59.0



112374 Crohns-F
7.3



112389 Match Control Crohns-F
0.5



112375 Crohns-F
4.4



112732 Match Control Crohns-F
6.1



112725 Crohns-M
0.9



112387 Match Control Crohns-M
2.5



112378 Crohns-M
0.4



112390 Match Control Crohns-M
7.5



112726 Crohns-M
2.3



112731 Match Control Crohns-M
4.7



112380 Ulcer Col-F
2.4



112734 Match Control Ulcer Col-F
24.1



112384 Ulcer Col-F
4.6



112737 Match Control Ulcer Col-F
1.6



112386 Ulcer Col-F
1.7



112738 Match Control Ulcer Col-F
3.2



112381 Ulcer Col-M
0.8



112735 Match Control Ulcer Col-M
3.8



112382 Ulcer Col-M
1.0



112394 Match Control Ulcer Col-M
1.9



112383 Ulcer Col-M
4.0



112736 Match Control Ulcer Col-M
0.6



112423 Psoriasis-F
8.9



112427 Match Control Psoriasis-F
28.5



112418 Psoriasis-M
2.1



112723 Match Control Psoriasis-M
6.9



112419 Psoriasis-M
4.2



112424 Match Control Psoriasis-M
4.4



112420 Psoriasis-M
5.4



112425 Match Control Psoriasis-M
33.2



104689 (MF) OA Bone-Backus
9.0



104690 (MF) Adj “Normal” Bone-Backus
8.1



104691 (MF) OA Synovium-Backus
54.0



104692 (BA) OA Cartilage-Backus
0.3



104694 (BA) OA Bone-Backus
20.3



104695 (BA) Adj “Normal” Bone-Backus
3.1



104696 (BA) OA Synovium-Backus
26.4



104700 (SS) OA Bone-Backus
2.8



104701 (SS) Adj “Normal” Bone-Backus
8.6



104702 (SS) OA Synovium-Backus
14.7



117093 OA Cartilage Rep7
2.1



112672 OA Bone5
11.9



112673 OA Synovium5
5.8



112674 OA Synovial Fluid cells5
6.4



117100 OA Cartilage Rep14
0.7



112756 OA Bone9
2.8



112757 OA Synovium9
0.8



112758 OA Synovial Fluid Cells9
6.1



117125 RA Cartilage Rep2
3.4



113492 Bone2 RA
40.3



113493 Synovium2 RA
15.8



113494 Syn Fluid Cells RA
25.2



113499 Cartilage4 RA
33.9



113500 Bone4 RA
42.6



113501 Synovium4 RA
26.6



113502 Syn Fluid Cells4 RA
20.0



113495 Cartilage3 RA
27.2



113496 Bone3 RA
32.8



113497 Synovium3 RA
19.3



113498 Syn Fluid Cells3 RA
39.5



117106 Normal Cartilage Rep20
0.0



113663 Bone3 Normal
0.7



113664 Synovium3 Normal
0.1



113665 Syn Fluid Cells3 Normal
0.3



117107 Normal Cartilage Rep22
2.2



113667 Bone4 Normal
4.1



113668 Synovium4 Normal
4.8



113669 Syn Fluid Cells4 Normal
5.8









Column A - Rel. Exp. (%) Ag1489, Run 226203351














TABLE AGD










Ardais Panel 1.1










Tissue Name
A














Lung adenocarcinoma SI A
2.0



Lung adenocarcinoma SI B
4.2



Lung adenocarcinoma SI B NAT
78.5



Lung adenocarcinoma SI C
0.1



Lung adenocarcinoma SI C NAT
52.1



Lung adenocarcinoma SII A
1.6



Lung adenocarcinoma SII A NAT
69.7



Lung adenocarcinoma SII C NAT
100.0



Lung adenocarcinoma SIII A
2.3



Lung adenocarcinoma SIII B
1.6



Lung adenocarcinoma SIII C
0.0



Lung SCC SI A
1.3



Lung SCC SI B NAT
61.6



Lung SCC SI C
14.5



Lung SCC SI C NAT
23.0



Lung SCC SI D
11.8



Lung SCC SI D NAT
16.6



Lung SCC SII A
79.6



Lung SCC SII B
0.7



Lung SCC SIII A
0.9



Lung SCC SIII A NAT
18.2









Column A - Rel. Exp. (%) Ag1489, Run 312115281














TABLE AGE










General_screening_panel_v1.4










Tissue Name
A














Adipose
4.7



Melanoma* Hs688(A).T
0.2



Melanoma* Hs688(B).T
0.4



Melanoma* M14
0.1



Melanoma* LOXIMVI
0.1



Melanoma* SK-MEL-5
0.2



Squamous cell carcinoma SCC-4
0.3



Testis Pool
1.5



Prostate ca.* (bone met) PC-3
0.3



Prostate Pool
0.2



Placenta
0.1



Uterus Pool
1.5



Ovarian ca. OVCAR-3
1.1



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.2



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.1



Ovary
3.8



Breast ca. MCF-7
0.7



Breast ca. MDA-MB-231
0.1



Breast ca. BT 549
0.2



Breast ca. T47D
0.3



Breast ca. MDA-N
0.0



Breast Pool
2.1



Trachea
7.0



Lung
8.5



Fetal Lung
100.0



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
10.7



Lung ca. SHP-77
0.1



Lung ca. A549
3.3



Lung ca. NCI-H526
0.2



Lung ca. NCI-H23
3.3



Lung ca. NCI-H460
0.1



Lung ca. HOP-62
2.6



Lung ca. NCI-H522
0.0



Liver
0.1



Fetal Liver
0.6



Liver ca. HepG2
0.0



Kidney Pool
8.0



Fetal Kidney
1.6



Renal ca. 786-0
0.3



Renal ca. A498
0.2



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.5



Gastric ca. (liver met.) NCI-N87
0.2



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.1



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
1.8



Colon ca. HCT-116
0.1



Colon ca. CaCo-2
3.8



Colon cancer tissue
0.3



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
2.0



Small Intestine Pool
4.2



Stomach Pool
1.5



Bone Marrow Pool
1.1



Fetal Heart
4.9



Heart Pool
2.7



Lymph Node Pool
2.2



Fetal Skeletal Muscle
8.2



Skeletal Muscle Pool
0.3



Spleen Pool
2.2



Thymus Pool
2.1



CNS cancer (glio/astro) U87-MG
0.3



CNS cancer (glio/astro) U-118-MG
0.4



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
3.9



CNS cancer (astro) SNB-75
10.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
4.0



Brain (Amygdala) Pool
0.5



Brain (cerebellum)
0.3



Brain (fetal)
0.4



Brain (Hippocampus) Pool
1.6



Cerebral Cortex Pool
1.1



Brain (Substantia nigra) Pool
0.5



Brain (Thalamus) Pool
1.4



Brain (whole)
0.6



Spinal Cord Pool
0.7



Adrenal Gland
3.8



Pituitary gland Pool
3.0



Salivary Gland
1.7



Thyroid (female)
1.0



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
2.4









Column A - Rel. Exp. (%) Ag4335, Run 222550589














TABLE AGF










Panel 2D











Tissue Name
A
B















Normal Colon
24.0
17.2



CC Well to Mod Diff (ODO3866)
1.4
0.4



CC Margin (ODO3866)
4.6
3.6



CC Gr.2 rectosigmoid (ODO3868)
0.4
0.7



CC Margin (ODO3868)
3.4
1.1



CC Mod Diff (ODO3920)
0.0
0.1



CC Margin (ODO3920)
5.0
4.7



CC Gr.2 ascend colon (ODO3921)
1.0
0.5



CC Margin (ODO3921)
4.1
5.0



CC from Partial Hepatectomy
2.2
0.9



(ODO4309) Mets



Liver Margin (ODO4309)
3.2
2.0



Colon mets to lung (OD04451-01)
0.4
0.7



Lung Margin (OD04451-02)
6.0
5.2



Normal Prostate 6546-1
1.1
0.9



Prostate Cancer (OD04410)
1.4
0.6



Prostate Margin (OD04410)
0.2
0.7



Prostate Cancer (OD04720-01)
0.9
0.6



Prostate Margin (OD04720-02)
1.0
1.0



Normal Lung 061010
100.0
100.0



Lung Met to Muscle (ODO4286)
0.8
1.0



Muscle Margin (ODO4286)
2.0
1.3



Lung Malignant Cancer (OD03126)
11.1
8.4



Lung Margin (OD03126)
64.2
51.8



Lung Cancer (OD04404)
6.6
5.1



Lung Margin (OD04404)
37.4
31.9



Lung Cancer (OD04565)
1.8
1.3



Lung Margin (OD04565)
19.3
20.6



Lung Cancer (OD04237-01)
2.0
2.2



Lung Margin (OD04237-02)
42.3
37.6



Ocular Mel Met to Liver (ODO4310)
0.0
0.2



Liver Margin (ODO4310)
2.3
0.5



Melanoma Mets to Lung (OD04321)
1.9
1.8



Lung Margin (OD04321)
49.0
37.9



Normal Kidney
5.4
4.8



Kidney Ca, Nuclear grade 2 (OD04338)
0.4
0.6



Kidney Margin (OD04338)
3.2
2.1



Kidney Ca Nuclear grade 1/2 (OD04339)
0.7
0.3



Kidney Margin (OD04339)
1.2
0.2



Kidney Ca, Clear cell type (OD04340)
0.4
0.4



Kidney Margin (OD04340)
5.1
4.2



Kidney Ca, Nuclear grade 3 (OD04348)
0.3
0.1



Kidney Margin (OD04348)
2.4
1.6



Kidney Cancer (OD04622-01)
0.1
0.2



Kidney Margin (OD04622-03)
0.0
0.1



Kidney Cancer (OD04450-01)
0.0
0.0



Kidney Margin (OD04450-03)
1.9
1.1



Kidney Cancer 8120607
0.0
0.0



Kidney Margin 8120608
0.1
0.0



Kidney Cancer 8120613
0.0
0.0



Kidney Margin 8120614
0.1
0.1



Kidney Cancer 9010320
1.1
1.0



Kidney Margin 9010321
0.1
0.2



Normal Uterus
3.1
2.3



Uterus Cancer 064011
24.5
18.8



Normal Thyroid
10.4
9.0



Thyroid Cancer 064010
0.3
0.0



Thyroid Cancer A302152
0.5
0.5



Thyroid Margin A302153
14.5
9.2



Normal Breast
32.1
31.9



Breast Cancer (OD04566)
0.5
0.4



Breast Cancer (OD04590-01)
9.3
4.4



Breast Cancer Mets (OD04590-03)
9.7
5.9



Breast Cancer Metastasis (OD04655-05)
8.2
5.8



Breast Cancer 064006
8.4
5.3



Breast Cancer 1024
9.6
9.2



Breast Cancer 9100266
1.7
2.2



Breast Margin 9100265
10.7
9.6



Breast Cancer A209073
11.0
10.2



Breast Margin A209073
13.6
12.5



Normal Liver
2.2
1.9



Liver Cancer 064003
0.6
0.4



Liver Cancer 1025
0.6
0.2



Liver Cancer 1026
0.0
0.1



Liver Cancer 6004-T
0.9
0.3



Liver Tissue 6004-N
0.2
0.2



Liver Cancer 6005-T
0.0
0.2



Liver Tissue 6005-N
0.0
0.0



Normal Bladder
2.4
0.5



Bladder Cancer 1023
0.5
0.9



Bladder Cancer A302173
33.4
31.4



Bladder Cancer (OD04718-01)
0.4
0.0



Bladder Normal Adjacent (OD04718-03)
17.4
17.6



Normal Ovary
22.4
14.9



Ovarian Cancer 064008
7.1
4.4



Ovarian Cancer (OD04768-07)
2.1
1.8



Ovary Margin (OD04768-08)
1.2
1.8



Normal Stomach
6.4
4.4



Gastric Cancer 9060358
3.1
1.4



Stomach Margin 9060359
1.3
2.3



Gastric Cancer 9060395
2.4
4.5



Stomach Margin 9060394
5.3
3.4



Gastric Cancer 9060397
0.3
0.7



Stomach Margin 9060396
0.7
0.1



Gastric Cancer 064005
6.8
4.4









Column A - Rel. Exp. (%) Ag1489, Run 152799815






Column B - Rel. Exp. (%) Ag1489, Run 152799848














TABLE AGG










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.6



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
1.2



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
1.5



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.9



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.3



Two Way MLR 3 day
1.7



Two Way MLR 5 day
0.5



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.5



PBMC PHA-L
0.0



Ramos (B cell) none
0.7



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.8



EOL-1 dbcAMP
34.2



EOL-1 dbcAMP PMA/ionomycin
18.6



Dendritic cells none
11.9



Dendritic cells LPS
0.7



Dendritic cells anti-CD40
2.7



Monocytes rest
1.4



Monocytes LPS
0.0



Macrophages rest
2.8



Macrophages LPS
1.1



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.9



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.5



HUVEC IL-11
0.0



Lung Microvascular EC none
1.1



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.6



Small airway epithelium TNFalpha + IL-1beta
0.7



Coronery artery SMC rest
1.9



Coronery artery SMC TNFalpha + IL-1beta
1.6



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
48.6



KU-812 (Basophil) PMA/ionomycin
26.1



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
6.0



NCI-H292 none
0.7



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.7



NCI-H292 IL-13
0.4



NCI-H292 IFN gamma
1.7



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
26.8



Lung fibroblast TNF alpha + IL-1 beta
2.0



Lung fibroblast IL-4
20.3



Lung fibroblast IL-9
26.2



Lung fibroblast IL-13
37.4



Lung fibroblast IFN gamma
44.1



Dermal fibroblast CCD1070 rest
3.0



Dermal fibroblast CCD1070 TNF alpha
0.7



Dermal fibroblast CCD1070 IL-1 beta
2.2



Dermal fibroblast IFN gamma
12.8



Dermal fibroblast IL-4
48.3



Dermal Fibroblasts rest
17.9



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.7



Colon
10.7



Lung
100.0



Thymus
27.9



Kidney
10.0









Column A - Rel. Exp. (%) Ag4335, Run 184793354














TABLE AGH










Panel 5D










Tissue Name
A














97457_Patient-02go_adipose
100.0



97476_Patient-07sk_skeletal muscle
23.3



97477_Patient-07ut_uterus
8.7



97478_Patient-07pl_placenta
7.9



97481_Patient-08sk_skeletal muscle
54.7



97482_Patient-08ut_uterus
1.4



97483_Patient-08pl_placenta
12.1



97486_Patient-09sk_skeletal muscle
3.3



97487_Patient-09ut_uterus
28.7



97488_Patient-09pl_placenta
2.6



97492_Patient-10ut_uterus
8.4



97493_Patient-10pl_placenta
9.5



97495_Patient-11go_adipose
39.5



97496_Patient-11sk_skeletal muscle
4.4



97497_Patient-11ut_uterus
11.1



97498_Patient-11pl_placenta
4.9



97500_Patient-12go_adipose
24.0



97501_Patient-12sk_skeletal muscle
6.0



97502_Patient-12ut_uterus
2.6



97503_Patient-12pl_placenta
2.9



94721_Donor 2 U - A_Mesenchymal Stem Cells
2.0



94722_Donor 2 U - B_Mesenchymal Stem Cells
0.7



94723_Donor 2 U - C_Mesenchymal Stem Cells
1.7



94709_Donor 2 AM - A_adipose
2.0



94710_Donor 2 AM - B_adipose
2.1



94711_Donor 2 AM - C_adipose
0.3



94712_Donor 2 AD - A_adipose
2.8



94713_Donor 2 AD - B_adipose
3.4



94714_Donor 2 AD - C_adipose
4.5



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.2



94743_Donor 3 U - B_Mesenchymal Stem Cells
1.1



94730_Donor 3 AM - A_adipose
4.2



94731_Donor 3 AM - B_adipose
2.0



94732_Donor 3 AM - C_adipose
1.8



94733_Donor 3 AD - A_adipose
0.5



94734_Donor 3 AD - B_adipose
0.3



94735_Donor 3 AD - C_adipose
0.2



77138_Liver_HepG2untreated
0.5



73556_Heart_Cardiac stromal cells (primary)
0.0



81735_Small Intestine
49.3



72409_Kidney_Proximal Convoluted Tubule
0.6



82685_Small intestine_Duodenum
5.2



90650_Adrenal_Adrenocortical adenoma
5.3



72410_Kidney_HRCE
0.0



72411_Kidney_HRE
0.8



73139_Uterus_Uterine smooth muscle cells
4.5









Column A - Rel. Exp. (%) Ag4335, Run 258657707







AI_comprehensive panel13 v1.0 Summary: Ag1489 Expression of this transcript was induced in most rheumatoid arthritis (RA) tissues and in synovium and bone tissues from osteoarthritis (OA) patients. This gene was also highly and consistently expressed in normal lung in 2 out of 3 normal lungs tissues on this panel and in panels 1.4 and 4.1. The expression in diseased lung was reduced or absent. These data show that lung expression of the protein encoded for by this transcript serves an important function that is lost in disease states, but that OA/RA promotes the expression of this gene in the joint. Therapies designed with the protein encoded by this transcript are important for the treatment of OA/RA and lung diseases such as chronic pulmonary obstructive disease, emphysema, allergy and asthma.


Ardais Panel 1.1 Summary: Ag1489 Highest expression is detected in normal adjacent lung tissue sample (CT=25). This gene is expressed at higher levels in normal tissue relative to the cancer samples. This expression profile is in agreement with results in panel 2D. This gene codes for a protein containing ig and leucine rich repeat regions and is similar to adlican. Members of this family are integral basement membrane proteins with a variety of functions, including matrix assembly and organization, regulation of cell growth, adhesion and migration. The LRR domain in particular is thought to play an important role in these processes (Hocking A M, Shinomura T, McQuillan D J., 1998, Leucine-rich repeat glycoproteins of the extracellular matrix. Matrix Biol. 17(1):1-19). The expression profile of this gene indicates an important role in lung biology and therefore modulation of this gene, expressed protein and/or use of agonists will be useful in the treatment of lung cancer.


General_screening13 panel_v1.4 Summary: Ag4335 Highest expression of this gene was seen in fetal lung (CT=25.4), with overall expression associated with normal tissues. Expression of this gene was higher in fetal lung relative to adult lung (CT=29) and in fetal skeletal muscle (CT=29) relative to adult skeletal muscle (CT=33). This gene or expressed protein is involved in the development and homeostasis of these organs. Therapeutic modulation of this gene and/or expressed protein is useful in the treatment of lung and skeletal muscle related diseases.


This gene was also expressed at significant levels in a variety of metabolic tissues including adipose, adult and fetal liver, adult and fetal heart, pituitary, thyroid and pancreas. Modulation of this gene and/or expressed protein is useful in the treatment of metabolic disorders, including obesity and diabetes.


Moderate to low expression of this gene was also seen in all the regions of brain examined including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. Therapeutic modulation of this gene or gene product is useful in the treatment of central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Panel 2D Summary: Ag1489 Highest expression of this gene was detected in normal lung (CTs=27.8). This gene showed higher expression in all the normal adjacent lung, kidney and colon tissues relative to cancer samples. Modulation of this gene, expressed protein and/or use of antibodies or agonists targeting this gene or its protein product are useful in the treatment of these cancers.


Panel 4.1D Summary: Ag4335 Highest expression of this gene was detected in normal lung (CT=31). This gene was also expressed in lung fibroblasts, EOL-1 cells and basophils. Expression of the gene was downregulated by TNF-alpha and IL1-beta in lung fibroblasts. Based on the expression profile in this panel as well as that in the A/I panel, therapeutics designed with the encoded protein are useful in the treatment of chronic obstructive pulmonary disease, emphysema, allergy and asthma.


Panel 5D Summary: Ag4335 Highest expression of this gene was detected in adipose tissue from a diabetic patient (CT=30.8). Significant expression of this gene was also seen in skeletal muscle, adipose, uterus, placenta and small intestine. Modulation of this gene or its expressed protein is useful in the treatment of metabolic diseases such as obesity and diabetes.


AH. CG5578401 and CG5578403: Nephrin-like


Expression of gene CG55784-03 and variant CG55784-01 was assessed using the primer-probe sets Ag2844 and Ag6457, described in Tables AHA and AHB. Results of the RTQ-PCR runs are shown in Tables AHC, AHD and AHE. Please note that the probe and primer set Ag6457 is specific to the CG55784-03 only.

TABLE AHAProbe Name Ag2844StartSEQ IDPrimersSequencesLengthPositionNoForward5′-ctttccactgctctgcaaag-3′2010531423ProbeTET-5′-aacccagctgtcacccagtacaggtg-3′-2610761424TAMRAReverse5′-gtcctgtacacctctccagatg-3′2211311425









TABLE AHB










Probe Name Ag6457















Start
SEQ ID



Primers
Sequences
Length
Position
No





Forward
5′-cgacttccagaccatctacaact-3′
23
1723
1426






Probe
TET-5′-cttcggctccgacactgagatcatc-3′-
25
1762
1427



TAMRA





Reverse
5′-ggcacagactcttgctcctt-3′
20
1793
1428
















TABLE AHC










General_screening_panel_v1.6










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
13.6



Melanoma* Hs688(B).T
15.9



Melanoma* M14
0.2



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.3



Testis Pool
0.0



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.8



Placenta
0.4



Uterus Pool
0.4



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
1.7



Ovarian ca. IGROV-1
1.7



Ovarian ca. OVCAR-8
1.8



Ovary
0.7



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.0



Trachea
2.0



Lung
0.4



Fetal Lung
5.4



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
3.7



Lung ca. SHP-77
0.0



Lung ca. A549
9.0



Lung ca. NCI-H526
1.1



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
0.1



Lung ca. HOP-62
0.5



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
0.0



Liver ca. HepG2
0.0



Kidney Pool
0.0



Fetal Kidney
0.1



Renal ca. 786-0
0.0



Renal ca. A498
3.2



Renal ca. ACHN
35.8



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Bladder
0.4



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
2.4



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
0.0



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.4



Small Intestine Pool
1.4



Stomach Pool
0.0



Bone Marrow Pool
0.3



Fetal Heart
1.5



Heart Pool
0.5



Lymph Node Pool
1.0



Fetal Skeletal Muscle
0.0



Skeletal Muscle Pool
0.0



Spleen Pool
1.1



Thymus Pool
0.4



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
8.0



CNS cancer (neuro; met) SK-N-AS
2.9



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.8



CNS cancer (glio) SNB-19
4.0



CNS cancer (glio) SF-295
0.7



Brain (Amygdala) Pool
32.3



Brain (cerebellum)
100.0



Brain (fetal)
41.2



Brain (Hippocampus) Pool
29.3



Cerebral Cortex Pool
35.8



Brain (Substantia nigra) Pool
25.5



Brain (Thalamus) Pool
56.3



Brain (whole)
79.0



Spinal Cord Pool
16.8



Adrenal Gland
2.0



Pituitary gland Pool
0.0



Salivary Gland
1.6



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
0.4









Column A - Rel. Exp. (%) Ag6457, Run 277250272














TABLE AHD










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
74.7



CD45RO CD4 lymphocyte act
0.3



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
2.4



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
4.4



Coronery artery SMC TNFalpha + IL-1beta
14.1



Astrocytes rest
2.9



Astrocytes TNFalpha + IL-1beta
5.2



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
0.0



NCI-H292 none
10.2



NCI-H292 IL-4
10.2



NCI-H292 IL-9
0.0



NCI-H292 IL-13
14.3



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
11.5



Lung fibroblast TNF alpha + IL-1 beta
6.6



Lung fibroblast IL-4
20.9



Lung fibroblast IL-9
5.1



Lung fibroblast IL-13
5.3



Lung fibroblast IFN gamma
23.2



Dermal fibroblast CCD1070 rest
100.0



Dermal fibroblast CCD1070 TNF alpha
59.5



Dermal fibroblast CCD1070 IL-1 beta
59.0



Dermal fibroblast IFN gamma
2.9



Dermal fibroblast IL-4
9.3



Dermal Fibroblasts rest
7.5



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.0



Colon
2.8



Lung
0.0



Thymus
0.0



Kidney
0.0









Column A - Rel. Exp.(%) Ag6457, Run 269261799














TABLE AHE










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
43.5



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
1.7



Coronery artery SMC TNFalpha + IL-1beta
5.1



Astrocytes rest
2.0



Astrocytes TNFalpha + IL-1beta
3.7



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
0.3



Lupus kidney
0.0



NCI-H292 none
1.8



NCI-H292 IL-4
27.2



NCI-H292 IL-9
3.7



NCI-H292 IL-13
17.8



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
4.3



Lung fibroblast TNF alpha + IL-1 beta
3.4



Lung fibroblast IL-4
12.5



Lung fibroblast IL-9
2.6



Lung fibroblast IL-13
5.3



Lung fibroblast IFN gamma
5.2



Dermal fibroblast CCD1070 rest
90.1



Dermal fibroblast CCD1070 TNF alpha
100.0



Dermal fibroblast CCD1070 IL-1 beta
39.8



Dermal fibroblast IFN gamma
1.6



Dermal fibroblast IL-4
4.5



IBD Colitis 2
0.0



IBD Crohn's
0.2



Colon
1.3



Lung
0.0



Thymus
0.4



Kidney
0.5









Column A - Rel. Exp.(%) Ag2844, Run 164299480







General_screening13 panel_v1.6 Summary: Ag6457 This probe/primer set was specific for the CG55784-03 gene variant. Expression of this gene was highest in cerebellum (CT=29.5). Highly brain-preferential expression of the CG55784-03 gene demonstrates a specific role for this gene product in the brain. This gene encodes a protein that is homologous to a neural cell adhesion molecule (NCAM). NCAM related proteins, such as Nr-CAM, play a critical role in neurite extension (Sakurai et al., J Cell Biol 2001 154:1259-73). Eintroduction of ligands specific for this gene product, such as contactin, in directed brain regions have utility in fostering focal neurite outgrowth and in therapeutically countering neurite degeneration of neurodegenerative diseases such as Alzheimer's, ataxias, and Parkinson's disease.


In addition, the expression of this gene was relatively high in the normal brain samples compared to the cancer cell lines derived from brain cancer. Expression of this gene is useful as a marker to differentiate between normal and cancerous tissue. There were also significantly higher levels of expression in renal cancer cell lines compared to a normal kidney sample. Expression of this gene is also useful as a marker in renal cancer.


Panel 4.1D Summary: Ag6457 This probe/primer set is specific for the CG55784-03 gene variant. Expression of this gene was highest in dermal fibroblasts (CT=31.8) and was relatively treatment independent. This gene was also expressed in CD45RA (naive) T cells. Therapeutic modulation of this gene or its protein product is useful in the treatment of psoriasis or diseases mediated by activated T cells.


Panel 4D Summary: Ag2844 This transcript was induced in IL-4 and IL-13 treated NCI-H292 cells, expressed constitutively in a dermal fibroblast cell line and was slightly induced by IL-4 in lung fibroblasts. CD45RA (naive) T cells also expressed the transcript. The transcript encodes a NCAM-like molecule. Based on the expression pattern of the transcript, the homology to NCAM protein, and the regulation of transcript expression by IL-4 and IL-13, therapeutics designed with the protein encoded for by this transcript are important in the treatment of asthma and COPD.


AI. CG55790-01 and CG55790-02: B7-H2B


Expression of gene CG55790-02 and variant CG55790-01 was assessed using the primer-probe sets Ag1845, Ag2589, Ag2621, Ag2915, and Ag8156, described in Tables AIA, AIB, AIC, AID, and AIE. Results of the RTQ-PCR runs are shown in Tables AIF, AIG, AIH, AII, All and AIKPlease note that only the probe and primer sets Ag2589, Ag2621, and Ag2915 correspond to the CG55790-01 variant.

TABLE AIAProbe Name Ag1845StartSEQ IDPrimersSequencesLengthPositionNoForward5′-catcacgtgtgagctcacagt-3′2174341429ProbeTET-5′-cttccacatggtgcactgctgct-3′-TAMRA2373931430Reverse5′-agaatttgcagacacagcaatt-3′2273661431









TABLE AIB










Probe Name Ag2589















Start
SEQ ID



Primers
Sequences
Length
Position
No





Forward
5′-gagctcaccttcacgtgtacat-3′
22
460
1432






Probe
TET-5′-ctaccccaggcccaacgtgtactg-3′-
24
492
1433



TAMRA





Reverse
5′-gctgttgtccgtcttattgatc-3′
22
516
1434
















TABLE AIC










Probe Name Ag2621















Start
SEQ ID



Primers
Sequences
Length
Position
No





Forward
5′-gagctcaccttcacgtgtacat-3′
22
460
1435






Probe
TET-5′-ctaccccaggcccaacgtgtactg-3′-
24
492
1436



TAMRA





Reverse
5′-gctgttgtccgtcttattgatc-3′
22
516
1437
















TABLE AID










Probe Name Ag2915















Start
SEQ ID



Primers
Sequences
Length
Position
No





Forward
5′-gagctcaccttcacgtgtacat-3′
22
460
1438






Probe
TET-5′-ctaccccaggcccaacgtgtactg-3′-
24
492
1439



TAMRA





Reverse
5′-gctgttgtccgtcttattgatc-3′
22
516
1440
















TABLE AIE










Probe Name Ag8156















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-tgccagctgtctttcagagt-3′
20
955
1441






Probe
TET-5′-ctttcaaaaggcaaaagtcccttgcc-3′-
26
984
1439



TAMRA





Reverse
5′-gcgtctccaggaacaacagt-3′
20
1017
1443
















TABLE AIF










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
8.5



110980 COPD-F
4.8



110968 COPD-M
6.7



110977 COPD-M
8.5



110989 Emphysema-F
12.9



110992 Emphysema-F
7.5



110993 Emphysema-F
8.5



110994 Emphysema-F
3.0



110995 Emphysema-F
14.9



110996 Emphysema-F
1.6



110997 Asthma-M
14.1



111001 Asthma-F
7.9



111002 Asthma-F
12.6



111003 Atopic Asthma-F
13.4



111004 Atopic Asthma-F
16.5



111005 Atopic Asthma-F
7.7



111006 Atopic Asthma-F
0.9



111417 Allergy-M
6.3



112347 Allergy-M
1.7



112349 Normal Lung-F
0.5



112357 Normal Lung-F
1.7



112354 Normal Lung-M
2.9



112374 Crohns-F
3.3



112389 Match Control Crohns-F
17.9



112375 Crohns-F
1.8



112732 Match Control Crohns-F
39.5



112725 Crohns-M
1.7



112387 Match Control Crohns-M
4.6



112378 Crohns-M
0.7



112390 Match Control Crohns-M
12.1



112726 Crohns-M
10.1



112731 Match Control Crohns-M
18.9



112380 Ulcer Col-F
9.6



112734 Match Control Ulcer Col-F
100.0



112384 Ulcer Col-F
17.1



112737 Match Control Ulcer Col-F
13.5



112386 Ulcer Col-F
1.8



112738 Match Control Ulcer Col-F
1.4



112381 Ulcer Col-M
7.3



112735 Match Control Ulcer Col-M
7.2



112382 Ulcer Col-M
12.5



112394 Match Control Ulcer Col-M
2.3



112383 Ulcer Col-M
6.3



112736 Match Control Ulcer Col-M
13.0



112423 Psoriasis-F
7.1



112427 Match Control Psoriasis-F
14.5



112418 Psoriasis-M
6.6



112723 Match Control Psoriasis-M
1.0



112419 Psoriasis-M
9.9



112424 Match Control Psoriasis-M
10.7



112420 Psoriasis-M
21.5



112425 Match Control Psoriasis-M
13.8



104689 (MF) OA Bone-Backus
17.1



104690 (MF) Adj “Normal” Bone-Backus
6.8



104691 (MF) OA Synovium-Backus
14.6



104692 (BA) OA Cartilage-Backus
0.4



104694 (BA) OA Bone-Backus
13.3



104695 (BA) Adj “Normal” Bone-Backus
9.3



104696 (BA) OA Synovium-Backus
7.9



104700 (SS) OA Bone-Backus
7.5



104701 (SS) Adj “Normal” Bone-Backus
7.7



104702 (SS) OA Synovium-Backus
24.5



117093 OA Cartilage Rep7
7.7



112672 OA Bone5
6.2



112673 OA Synovium5
1.1



112674 OA Synovial Fluid cells5
2.6



117100 OA Cartilage Rep14
4.3



112756 OA Bone9
14.6



112757 OA Synovium9
7.1



112758 OA Synovial Fluid Cells9
4.2



117125 RA Cartilage Rep2
4.7



113492 Bone2 RA
4.8



113493 Synovium2 RA
3.6



113494 Syn Fluid Cells RA
7.5



113499 Cartilage4 RA
9.6



113500 Bone4 RA
10.7



113501 Synovium4 RA
6.9



113502 Syn Fluid Cells4 RA
5.4



113495 Cartilage3 RA
4.0



113496 Bone3 RA
7.2



113497 Synovium3 RA
4.4



113498 Syn Fluid Cells3 RA
6.2



117106 Normal Cartilage Rep20
7.3



113663 Bone3 Normal
0.2



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
0.4



117107 Normal Cartilage Rep22
3.0



113667 Bone4 Normal
4.3



113668 Synovium4 Normal
2.7



113669 Syn Fluid Cells4 Normal
7.0









Column A - Rel. Exp.(%) Ag1845, Run 217699370














TABLE AIG










CNS_neurodegeneration_v1.0













Tissue Name
A
B
C
D
E
F
















AD 1 Hippo
37.6
19.1
26.4
26.1
42.3
37.9


AD 2 Hippo
26.4
11.1
26.6
21.2
27.0
33.4


AD 3 Hippo
39.5
15.6
24.1
15.1
20.4
32.3


AD 4 Hippo
24.3
10.6
16.8
10.7
20.9
28.5


AD 5 Hippo
29.5
23.8
26.2
22.7
32.3
28.3


AD 6 Hippo
62.0
45.4
57.4
45.1
56.3
75.8


Control 2 Hippo
57.4
22.4
45.7
29.1
41.5
50.7


Control 4 Hippo
22.8
10.9
27.4
20.4
24.5
25.3


Control (Path) 3 Hippo
22.7
100.0
14.6
12.3
14.7
12.5


AD 1 Temporal Ctx
48.0
27.2
33.9
27.9
33.7
44.8


AD 2 Temporal Ctx
39.0
30.1
25.2
21.5
33.7
29.9


AD 3 Temporal Ctx
42.3
19.8
19.5
14.8
13.4
16.3


AD 4 Temporal Ctx
35.1
14.7
24.3
22.7
33.7
26.2


AD 5 Inf Temporal Ctx
70.2
28.3
50.3
45.4
63.7
60.3


AD 5 Sup Temporal Ctx
48.6
17.8
37.4
32.1
45.1
35.1


AD 6 Inf Temporal Ctx
100.0
19.6
100.0
100.0
100.0
100.0


AD 6 Sup Temporal Ctx
57.4
27.0
47.3
33.4
49.0
59.9


Control 1 Temporal Ctx
11.8
9.7
14.8
8.8
14.2
14.0


Control 2 Temporal Ctx
33.7
23.0
30.8
26.8
11.3
45.4


Control 3 Temporal Ctx
20.6
15.9
18.6
13.7
17.2
18.8


Control 3 Temporal Ctx
21.9
4.5
14.9
12.5
26.6
23.7


Control (Path) 1
27.2
26.1
27.5
19.6
28.5
31.4


Temporal Ctx


Control (Path) 2
16.7
7.2
18.3
16.5
17.7
23.2


Temporal Ctx


Control (Path) 3
23.2
12.4
7.9
6.0
7.2
10.8


Temporal Ctx


Control (Path) 4
17.4
35.4
15.1
12.9
17.3
19.6


Temporal Ctx


AD 1 Occipital Ctx
30.4
9.2
22.2
14.5
25.0
23.3


AD 2 Occipital Ctx
1.3
26.1
1.9
14.8
2.5
1.5


(Missing)


AD 3 Occipital Ctx
26.8
16.0
17.3
14.6
23.0
20.9


AD 4 Occipital Ctx
45.1
13.0
27.9
22.5
27.2
0.1


AD 5 Occipital Ctx
33.0
57.8
27.5
31.2
27.9
32.1


AD 6 Occipital Ctx
47.3
22.7
36.6
19.3
40.6
44.1


Control 1 Occipital Ctx
27.5
16.2
16.4
11.3
12.8
16.5


Control 2 Occipital Ctx
39.8
15.1
28.3
29.3
42.0
42.3


Control 3 Occipital Ctx
20.7
16.0
18.7
13.0
18.6
16.4


Control 4 Occipital Ctx
35.1
23.5
17.6
19.6
24.3
22.5


Control (Path) 1
47.0
23.3
44.4
35.8
47.0
51.8


Occipital Ctx


Control (Path) 2
18.3
32.5
21.9
17.6
31.4
23.8


Occipital Ctx


Control (Path) 3
23.8
8.2
12.6
15.2
12.2
22.5


Occipital Ctx


Control (Path) 4
22.8
14.6
20.9
17.8
21.0
23.2


Occipital Ctx


Control 1 Parietal Ctx
34.6
13.6
25.0
20.7
22.5
28.3


Control 2 Parietal Ctx
48.0
18.0
36.9
25.3
35.4
40.1


Control 3 Parietal Ctx
37.6
30.6
26.4
20.3
31.4
24.1


Control (Path) 1
29.5
11.9
28.7
23.3
28.7
31.9


Parietal Ctx


Control (Path) 2
24.5
21.8
19.8
17.3
29.9
28.3


Parietal Ctx


Control (Path) 3
15.6
17.6
13.2
9.0
8.4
16.8


Parietal Ctx


Control (Path) 4
23.8
11.7
22.7
21.0
23.0
22.5


Parietal Ctx







Column A - Rel. Exp. (%) Ag1845, Run 207807655





Column B - Rel. Exp. (%) Ag1845, Run 224079124





Column C - Rel. Exp. (%) Ag2589, Run 208776915





Column D - Rel. Exp. (%) Ag2621, Run 208393684





Column E - Rel. Exp. (%) Ag2915, Run 209735956





Column F - Rel. Exp. (%) Ag2915, Run 266906552














TABLE AIH










Panel 1.3D












Tissue Name
A
B
C
D
E















Liver adenocarcinoma
3.9
4.7
23.8
17.6
22.8


Pancreas
0.9
0.3
0.8
1.6
2.3


Pancreatic ca. CAPAN 2
0.0
0.0
2.6
0.5
1.0


Adrenal gland
1.6
2.1
3.7
3.2
1.9


Thyroid
2.2
2.9
4.2
5.3
3.3


Salivary gland
2.0
3.1
6.3
4.0
4.2


Pituitary gland
0.9
1.1
2.0
2.0
1.4


Brain (fetal)
0.2
0.9
4.6
6.0
3.3


Brain (whole)
31.2
31.4
90.8
90.8
77.4


Brain (amygdala)
28.5
24.7
34.9
31.4
34.4


Brain (cerebellum)
3.0
1.5
21.6
18.6
20.7


Brain (hippocampus)
100.0
100.0
39.2
29.9
27.7


Brain (substantia nigra)
26.1
21.3
86.5
60.7
67.8


Brain (thalamus)
29.9
31.0
89.5
49.7
59.0


Cerebral Cortex
20.0
16.4
46.3
33.2
38.4


Spinal cord
15.0
12.7
29.9
20.2
31.0


glio/astro U87-MG
2.6
3.5
18.7
13.3
18.9


glio/astro U-118-MG
1.3
0.3
0.6
1.6
1.6


astrocytoma SW 1783
0.0
0.1
1.5
1.0
0.3


neuro*; met SK-N-AS
0.0
0.0
0.0
0.0
0.0


astrocytoma SF-539
5.5
4.2
28.9
15.3
21.8


astrocytoma SNB-75
1.9
1.3
10.7
5.4
5.5


glioma SNB-19
0.6
1.6
2.4
1.7
3.6


glioma U251
0.4
0.9
10.0
7.1
5.0


glioma SF-295
11.3
12.1
24.1
17.1
25.5


Heart (fetal)
12.9
8.2
29.3
24.5
31.4


Heart
1.9
2.0
12.8
8.5
12.2


Skeletal muscle (fetal)
54.7
48.6
30.6
34.9
36.1


Skeletal muscle
1.0
0.6
3.1
4.4
3.2


Bone marrow
3.2
2.6
3.3
3.5
3.6


Thymus
4.9
5.3
10.2
11.1
11.3


Spleen
15.9
15.8
11.2
10.7
15.3


Lymph node
11.4
11.0
27.0
29.5
28.7


Colorectal
4.5
6.5
9.5
8.1
7.5


Stomach
9.2
8.5
9.5
8.2
9.7


Small intestine
5.7
6.4
6.8
4.6
5.7


Colon ca. SW480
2.0
4.7
7.3
6.3
7.9


Colon ca.* SW620(SW480 met)
0.8
2.1
12.2
26.4
19.2


Colon ca. HT29
0.3
0.4
5.2
4.2
4.3


Colon ca. HCT-116
4.9
6.5
12.2
14.7
14.2


Colon ca. CaCo-2
15.1
12.4
30.8
28.5
29.7


Colon ca. tissue(ODO3866)
5.9
5.6
17.3
24.3
19.3


Colon ca. HCC-2998
5.0
9.9
30.8
31.9
35.8


Gastric ca.* (liver met) NCI-N87
3.3
6.0
6.1
7.4
6.2


Bladder
2.1
1.9
14.3
9.7
15.7


Trachea
9.7
5.7
3.0
2.3
2.3


Kidney
5.5
3.9
24.0
23.2
21.9


Kidney (fetal)
11.8
14.4
100.0
100.0
85.9


Renal ca. 786-0
6.5
5.2
27.4
28.3
33.2


Renal ca. A498
8.2
9.6
19.3
21.5
21.8


Renal ca. RXF 393
3.5
3.6
50.0
55.9
48.6


Renal ca. ACHN
3.2
4.7
8.7
7.6
9.2


Renal ca. UO-31
3.0
50.0
3.3
4.2
4.2


Renal ca. TK-10
4.4
4.9
18.0
13.7
15.5


Liver
3.2
1.2
5.6
4.4
8.0


Liver (fetal)
2.7
2.5
1.9
5.6
2.6


Liver ca. (hepatoblast) HepG2
1.9
3.0
10.8
8.7
9.6


Lung
7.5
7.7
12.8
13.9
17.0


Lung (fetal)
7.0
8.2
13.3
6.8
5.0


Lung ca. (small cell) LX-1
2.6
2.3
9.0
6.1
9.6


Lung ca. (small cell) NCI-H69
0.7
0.3
1.5
1.0
0.5


Lung ca. (s. cell var.) SHP-77
0.2
0.0
0.0
0.0
0.5


Lung ca. (large cell)NCI-H460
0.5
1.1
0.6
0.0
0.7


Lung ca. (non-sm. cell) A549
0.3
0.2
4.0
4.0
5.7


Lung ca. (non-s. cell) NCI-H23
4.0
3.3
8.4
6.6
7.8


Lung ca. (non-s. cell) HOP-62
1.5
1.7
1.5
2.4
3.3


Lung ca. (non-s. cl) NCI-H522
4.0
8.4
13.4
12.3
12.0


Lung ca. (squam.) SW 900
2.0
0.4
5.9
5.8
4.6


Lung ca. (squam.) NCI-H596
0.0
0.0
0.0
0.4
1.1


Mammary gland
12.3
7.6
32.1
26.2
32.1


Breast ca.* (pl. ef) MCF-7
15.3
10.5
76.3
79.0
100.0


Breast ca.* (pl. ef) MDA-MB-231
1.8
2.0
6.2
6.7
6.5


Breast ca.* (pl. ef) T47D
4.5
6.4
35.8
31.9
37.4


Breast ca. BT-549
0.9
1.4
9.1
6.3
6.2


Breast ca. MDA-N
0.6
0.9
2.9
4.3
6.5


Ovary
2.2
4.0
5.0
6.3
6.3


Ovarian ca. OVCAR-3
8.7
6.9
26.2
31.6
41.2


Ovarian ca. OVCAR-4
1.6
1.9
23.8
11.5
20.2


Ovarian ca. OVCAR-5
1.9
2.6
20.7
17.6
14.7


Ovarian ca. OVCAR-8
0.9
1.8
2.5
2.7
1.3


Ovarian ca. IGROV-1
1.9
1.0
10.7
8.1
9.9


Ovarian ca.* (ascites) SK-OV-3
0.5
1.2
16.7
12.0
10.7


Uterus
1.9
3.4
2.4
4.2
4.1


Placenta
1.6
1.6
1.8
1.5
1.4


Prostate
7.6
3.2
4.6
2.9
3.8


Prostate ca.* (bone met)PC-3
6.5
7.0
19.9
17.2
19.3


Testis
4.0
3.3
3.0
1.0
1.9


Melanoma Hs688(A).T
0.0
0.0
0.0
0.0
0.0


Melanoma* (met) Hs688(B).T
0.0
0.0
0.0
0.0
0.0


Melanoma UACC-62
0.2
0.2
1.0
3.6
3.8


Melanoma M14
0.0
0.0
0.0
0.0
0.0


Melanoma LOX IMVI
0.5
0.4
0.0
0.0
0.0


Melanoma* (met) SK-MEL-5
1.1
0.2
1.3
2.2
2.4


Adipose
9.7
11.0
32.8
29.9
31.4







Column A - Rel. Exp. (%) Ag1845, Run 148648438





Column B - Rel. Exp. (%) Ag1845, Run 149951496





Column C - Rel. Exp. (%) Ag2589, Run 167660070





Column D - Rel. Exp. (%) Ag2621, Run 167644903





Column E - Rel. Exp. (%) Ag2915, Run 167646705














TABLE AII










Panel 2D











Tissue Name
A
B















Normal Colon
35.1
24.5



CC Well to Mod Diff (ODO3866)
19.3
16.6



CC Margin (ODO3866)
9.4
11.9



CC Gr.2 rectosigmoid (ODO3868)
14.0
11.7



CC Margin (ODO3868)
1.4
1.9



CC Mod Diff (ODO3920)
9.6
7.1



CC Margin (ODO3920)
6.1
8.0



CC Gr.2 ascend colon (ODO3921)
58.2
47.6



CC Margin (ODO3921)
16.3
8.2



CC from Partial Hepatectomy
28.5
22.5



(ODO4309) Mets



Liver Margin (ODO4309)
18.4
8.1



Colon mets to lung (OD04451-01)
12.0
15.7



Lung Margin (OD04451-02)
6.5
3.9



Normal Prostate 6546-1
23.2
17.3



Prostate Cancer (OD04410)
29.5
12.6



Prostate Margin (OD04410)
18.3
12.7



Prostate Cancer (OD04720-01)
39.0
28.9



Prostate Margin (OD04720-02)
32.8
32.3



Normal Lung 061010
47.3
42.9



Lung Met to Muscle (ODO4286)
16.5
19.6



Muscle Margin (ODO4286)
3.5
4.5



Lung Malignant Cancer (OD03126)
25.3
26.2



Lung Margin (OD03126)
9.5
12.3



Lung Cancer (OD04404)
19.8
13.9



Lung Margin (OD04404)
12.3
10.1



Lung Cancer (OD04565)
4.8
3.8



Lung Margin (OD04565)
13.7
8.3



Lung Cancer (OD04237-01)
11.0
9.9



Lung Margin (OD04237-02)
21.0
23.0



Ocular Mel Met to Liver (ODO4310)
4.9
3.5



Liver Margin (ODO4310)
5.2
4.0



Melanoma Mets to Lung (OD04321)
1.3
3.5



Lung Margin (OD04321)
18.2
23.7



Normal Kidney
65.5
50.3



Kidney Ca, Nuclear grade 2 (OD04338)
49.0
36.1



Kidney Margin (OD04338)
48.3
33.2



Kidney Ca Nuclear grade 1/2 (OD04339)
19.8
14.0



Kidney Margin (OD04339)
81.8
65.1



Kidney Ca, Clear cell type (OD04340)
100.0
100.0



Kidney Margin (OD04340)
82.4
66.0



Kidney Ca, Nuclear grade 3 (OD04348)
9.9
9.9



Kidney Margin (OD04348)
45.1
42.6



Kidney Cancer (OD04622-01)
24.3
14.2



Kidney Margin (OD04622-03)
9.4
10.0



Kidney Cancer (OD04450-01)
6.5
6.2



Kidney Margin (OD04450-03)
22.4
33.0



Kidney Cancer 8120607
33.4
24.5



Kidney Margin 8120608
44.4
28.3



Kidney Cancer 8120613
30.4
17.8



Kidney Margin 8120614
62.9
51.8



Kidney Cancer 9010320
36.3
26.4



Kidney Margin 9010321
46.7
38.2



Normal Uterus
2.1
3.8



Uterus Cancer 064011
17.8
14.5



Normal Thyroid
8.7
5.0



Thyroid Cancer 064010
10.2
8.9



Thyroid Cancer A302152
2.9
4.5



Thyroid Margin A302153
10.3
7.3



Normal Breast
18.0
17.1



Breast Cancer (OD04566)
15.7
11.5



Breast Cancer (OD04590-01)
42.6
49.7



Breast Cancer Mets (OD04590-03)
42.6
31.2



Breast Cancer Metastasis (OD04655-05)
33.4
23.3



Breast Cancer 064006
15.6
15.3



Breast Cancer 1024
64.2
49.0



Breast Cancer 9100266
12.1
12.2



Breast Margin 9100265
17.7
20.0



Breast Cancer A209073
27.5
33.2



Breast Margin A209073
22.8
27.7



Normal Liver
14.4
8.1



Liver Cancer 064003
4.9
4.1



Liver Cancer 1025
9.3
8.3



Liver Cancer 1026
8.4
8.8



Liver Cancer 6004-T
10.2
11.9



Liver Tissue 6004-N
2.5
2.0



Liver Cancer 6005-T
7.3
6.2



Liver Tissue 6005-N
2.9
3.2



Normal Bladder
17.3
15.2



Bladder Cancer 1023
11.0
12.3



Bladder Cancer A302173
46.3
47.0



Bladder Cancer (OD04718-01)
65.1
42.3



Bladder Normal Adjacent (OD04718-03)
11.6
6.7



Normal Ovary
2.9
2.3



Ovarian Cancer 064008
32.5
28.1



Ovarian Cancer (OD04768-07)
9.3
8.0



Ovary Margin (OD04768-08)
8.8
6.3



Normal Stomach
15.4
16.3



Gastric Cancer 9060358
10.4
4.0



Stomach Margin 9060359
15.6
20.6



Gastric Cancer 9060395
25.9
26.4



Stomach Margin 9060394
35.8
29.9



Gastric Cancer 9060397
79.0
64.2



Stomach Margin 9060396
10.9
8.4



Gastric Cancer 064005
23.8
27.5









Column A - Rel. Exp. (%) Ag1845, Run 148648439






Column B - Rel. Exp. (%) Ag1845, Run 149957753














TABLE AIJ










Panel 4.1D










Tissue Name
A














Secondary Th1 act
6.4



Secondary Th2 act
3.5



Secondary Tr1 act
0.6



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.8



Primary Th2 act
6.3



Primary Tr1 act
5.1



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
2.6



CD45RO CD4 lymphocyte act
2.1



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.8



CD4 lymphocyte none
0.4



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.7



LAK cells rest
0.9



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.2



LAK cells PMA/ionomycin
1.2



NK Cells IL-2 rest
0.4



Two Way MLR 3 day
2.1



Two Way MLR 5 day
0.2



Two Way MLR 7 day
0.8



PBMC rest
2.2



PBMC PWM
1.3



PBMC PHA-L
0.0



Ramos (B cell) none
6.3



Ramos (B cell) ionomycin
7.8



B lymphocytes PWM
0.3



B lymphocytes CD40L and IL-4
3.1



EOL-1 dbcAMP
36.3



EOL-1 dbcAMP PMA/ionomycin
47.0



Dendritic cells none
9.2



Dendritic cells LPS
2.8



Dendritic cells anti-CD40
0.4



Monocytes rest
0.4



Monocytes LPS
5.5



Macrophages rest
1.4



Macrophages LPS
2.7



HUVEC none
0.3



HUVEC starved
1.0



HUVEC IL-1beta
2.2



HUVEC IFN gamma
1.2



HUVEC INF alpha + IFN gamma
2.8



HUVEC TNF alpha + IL4
2.9



HUVEC IL-11
0.0



Lung Microvascular EC none
1.3



Lung Microvascular EC TNFalpha + IL-1beta
11.8



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
4.0



Bronchial epithelium TNFalpha + IL1beta
0.3



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
1.0



Coronery artery SMC rest
0.7



Coronery artery SMC TNFalpha + IL-1beta
1.2



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
14.0



KU-812 (Basophil) rest
2.5



KU-812 (Basophil) PMA/ionomycin
1.3



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.2



Liver cirrhosis
0.4



NCI-H292 none
0.2



NCI-H292 IL-4
0.5



NCI-H292 IL-9
0.2



NCI-H292 IL-13
0.8



NCI-H292 IFN gamma
0.5



HPAEC none
0.7



HPAEC TNF alpha + IL-1 beta
100.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
6.4



Dermal fibroblast CCD1070 IL-1 beta
0.6



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



Dermal Fibroblasts rest
0.0



Neutrophils TNFa + LPS
0.9



Neutrophils rest
0.0



Colon
0.0



Lung
0.0



Thymus
1.0



Kidney
2.7









Column A - Rel. Exp.(%) Ag8156, Run 319806134














TABLE AIK










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
26.6



Colon NAT 1
5.8



Colon cancer 2
7.1



Colon NAT 2
3.6



Colon cancer 3
26.6



Colon NAT 3
7.3



Colon malignant cancer 4
23.8



Colon NAT 4
1.5



Lung cancer 1
5.8



Lung NAT 1
1.3



Lung cancer 2
42.6



Lung NAT 2
1.7



Squamous cell carcinoma 3
10.0



Lung NAT 3
0.6



Metastatic melanoma 1
3.1



Melanoma 2
6.4



Melanoma 3
3.3



Metastatic melanoma 4
4.6



Metastatic melanoma 5
6.0



Bladder cancer 1
0.3



Bladder NAT 1
0.0



Bladder cancer 2
0.8



Bladder NAT 2
0.2



Bladder NAT 3
0.3



Bladder NAT 4
3.8



Prostate adenocarcinoma 1
4.4



Prostate adenocarcinoma 2
0.9



Prostate adenocarcinoma 3
3.4



Prostate adenocarcinoma 4
8.0



Prostate NAT 5
2.2



Prostate adenocarcinoma 6
0.8



Prostate adenocarcinoma 7
2.9



Prostate adenocarcinoma 8
0.4



Prostate adenocarcinoma 9
12.4



Prostate NAT 10
0.4



Kidney cancer 1
24.0



Kidney NAT 1
9.6



Kidney cancer 2
100.0



Kidney NAT 2
13.5



Kidney cancer 3
31.0



Kidney NAT 3
11.8



Kidney cancer 4
27.0



Kidney NAT 4
13.7









Column A - Rel. Exp. (%)Ag2915, Run 260443054







AI_comprehensive panel_v1.0 Summary: Ag1845 This transcript was expressed at low levels in many different disease tissues. In comparison, normal lung and joint tissues expressed none or extremely low levels of this transcript. Since this transcript was expressed in monocytes, and matched control tissues contain these inflammatory cells (psoriasis, Crohn's and ulcerative colitis), this transcript is detected at these sites. This transcript encodes B7-H2, which has been shown to be important in antigen presentation. It is a ligand for ICOS and serves as a costimulatory molecule. Therapeutics designed with the transcript are useful for the reduction or inhibition of antigen presentation and in the treatment of diseases such as asthma, IBD, psoriasis and arthritis in which T cells are chronically stimulated.


CNS_neurodegeneration_v1.0 Summary: Ag1845/Ag2589/Ag2621/Ag2915 The expression of this gene was up-regulated in the temporal cortex of Alzheimer's disease patients when compared to non-demented controls. This difference was apparent when the data were analyzed via ANCOVA, using overall RNA quality and/or quantity as a covariate. The up-regulation of this gene was most apparent in the variant detected by Ag1845. The temporal cortex is a region that shows degeneration at the mid-stages of this disease. Thus, the phenomenon of neurodegeneration was captured in this region, as opposed to the hippocampus and entorhinal cortex where a large number of neurons are already lost by the time of death in AD. Furthermore, in the occipital cortex (where neurodegeneration does not occur in Alzheimer's) this gene was not found to be up-regulated in the same patients. Taken together, these data demonstrate that this gene is useful as a marker of Alzheimer's-like neurodegeneration, and is involved in the process of neurodegeneration. This gene is a form of the B7 protein (B7-H2B), which plays a role in inflammation. Neuroinflammation has been implicated in AD, to the extent that long-term usage of anti-inflammatory agents has been correlated with a reduced incidence of Alzheimer's in retrospective studies. This gene therefore represents an excellent drug target for the treatment of Alzheimer's disease, and any other neuroinflammatory condition.


Panel 1.3D Summary: Ag1845/Ag2589/Ag2621/Ag2915 Highest expression of this gene was seen in the brain, fetal kidney, and a breast cancer cell line. Higher levels of expression were also consistently seen in fetal skeletal muscle (CTs=29-30), when compared to expression in adult skeletal muscle (CTs=33-35). Thus, expression of this gene is useful as a marker to differentiate between the adult and fetal sources of this tissue. The relative overexpression of this gene in fetal sekeltal muscle shows that the protein product enhances muscle growth or development in the fetus and also acts in a regenerative capacity in the adult. Therapeutic modulation of the protein encoded by this gene is useful in the treatment of lung related diseases.


This gene product was also moderately expressed in pancreas, adrenal, thyroid, pituitary, adult and fetal liver, adult and fetal heart, and adipose. Based on its expression profile in metabolic tissues, this gene product is useful in the diagnosis and/or treatment of metabolic disease, including obesity and diabetes.


Panel 2D Summary: Ag1845 The expression of this gene was highest in a sample derived from a kidney cancer (CTs=28). There was substantial expression associated with other samples derived from kidney tissue, bladder cancer and breast cancer. The expression of this gene is useful as a marker to distinguish this kidney cancer sample from other samples in the panel. Therapeutic modulation of this gene, through the use of small molecule drugs, protein therapeutics or antibodies is beneficial for the treatment of kidney cancer, breast cancer or bladder cancer.


Panel 4.1D Summary: Ag8156 Highest expression of this gene was detected in TNF alpha+IL-1 beta activated HPAEC cells (CT=30.9). Low expression of this gene was also seen in activated lung microvascular endothelial cells. Expression of this gene was very low or undetectable in resting endothelial cells. Low expression of this gene was also seen in activated astrocytes, eosinophils, Ramos B cells, and activated primary Th2 and secondary Th1 cells. This gene codes for B7-H2B gene. B7-H2 is a recently cloned costimulatory ligand that belongs to B7 family. B7-H2 binds to ICOS, an inducible costimulatory receptor expressed on activated, but not resting T cells. (Wang S, Blood 2000 October 15;96(8):2808-13) Interaction of B7-H2 and ICOS in the presence of the TCR stimuli results in an augmented proliferative responses and cytokine production. The data show that circumstances B7-H2 more effectively costimulates Th2 responses. The B7-H2/ICOS pathway plays an important role in antibody response and germinal center formation. (Tesciuba. J Immunol 2001 August 15;167(4):1996-2003) Thus, B7-H2 is useful in the generation of therapeutics for diseases with antibody-mediated and Th2-mediated pathogenesis. Also, antibodies and small molecules that antagonize the function of the B7-H2B protein are useful for the reduction or elimination of the symptoms in patients with autoimmune and inflammatory diseases that involve endothelial cells, such as lupus erythematosus, asthma, emphysema, Crohn's disease, ulcerative colitis, rheumatoid arthritis, osteoarthritis, and psoriasis.


general oncology screening panel_v—2.4 Summary: Ag2915 Highest expression of this gene was detected in a kidney cancer sample (CT=28.2). Moderate to low expression of this gene was also seen in cancer and normal samples derived from kidney, colon, metastatic melanoma, prostate and lung. Expression of this gene was higher in cancer samples. Therefore, expression of this gene is useful as a diagnostic marker to detect the presence of these cancers. Therapeutic modulation of this gene or its protein product through the use of antibodies or small molecule drug is useful in the treatment of kidney, colon, metastatic melanoma, prostate and lung cancers.


AJ. CG55906-01: S3-12


Expression of gene CG55906-01 was assessed using the primer-probe sets Ag2840 and Ag6179, described in Tables AJA and AJB. Results of the RTQ-PCR runs are shown in Tables AJC, AMD, AME, AJF and AJG.

TABLE AJAProbe Name Ag2840StartSEQ IDPrimersSequencesLengthPositionNoForward5′-tctatggtcatgggtacgaaag-3′2211901444ProbeTET-5′-acacgatgtccactgggctcacag-3′-2412121445TAMRAReverse5′-gttgtgttcagcccagtttg-3′2012651446









TABLE AJB










Probe Name Ag6179















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-ttctcactatgtctgctcca-3′
20
123
1447






Probe
TET-5′-atgcagaccctgggcagcttct-3′-TAMRA
22
194
1448





Reverse
5′-ttccgggcagagctgaa-3′
17
233
1449
















TABLE AJC










General_screening_panel_v1.5










Tissue Name
A














Adipose
73.7



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.2



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
0.9



Prostate ca.* (bone met) PC-3
5.1



Prostate Pool
2.6



Placenta
0.1



Uterus Pool
1.3



Ovarian ca. OVCAR-3
0.3



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
3.4



Ovarian ca. IGROV-1
0.1



Ovarian ca. OVCAR-8
0.3



Ovary
0.7



Breast ca. MCF-7
0.1



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
0.0



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
6.7



Trachea
5.0



Lung
0.2



Fetal Lung
0.1



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.1



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.1



Lung ca. A549
0.0



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
0.1



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
0.8



Fetal Liver
0.9



Liver ca. HepG2
1.4



Kidney Pool
13.7



Fetal Kidney
0.1



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
1.0



Bladder
6.5



Gastric ca. (liver met.) NCI-N87
1.7



Gastric ca. KATO III
0.3



Colon ca. SW-948
0.1



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.8



Colon ca. HCT-116
0.4



Colon ca. CaCo-2
0.1



Colon cancer tissue
17.6



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
7.7



Small Intestine Pool
6.1



Stomach Pool
3.7



Bone Marrow Pool
1.7



Fetal Heart
0.8



Heart Pool
7.9



Lymph Node Pool
9.1



Fetal Skeletal Muscle
2.5



Skeletal Muscle Pool
100.0



Spleen Pool
0.0



Thymus Pool
5.9



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.1



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.3



Brain (Amygdala) Pool
0.8



Brain (cerebellum)
0.7



Brain (fetal)
0.1



Brain (Hippocampus) Pool
0.8



Cerebral Cortex Pool
0.5



Brain (Substantia nigra) Pool
0.9



Brain (Thalamus) Pool
0.8



Brain (whole)
0.2



Spinal Cord Pool
3.5



Adrenal Gland
3.7



Pituitary gland Pool
0.0



Salivary Gland
1.8



Thyroid (female)
0.5



Pancreatic ca. CAPAN2
1.0



Pancreas Pool
1.8









Column A - Rel. Exp.(%) Ag6179, Run 256555247














TABLE AJD










Panel 2D










Tissue Name
A














Normal Colon
41.8



CC Well to Mod Diff (ODO3866)
4.3



CC Margin (ODO3866)
6.9



CC Gr.2 rectosigmoid (ODO3868)
1.1



CC Margin (ODO3868)
4.5



CC Mod Diff (ODO3920)
0.0



CC Margin (ODO3920)
0.0



CC Gr.2 ascend colon (ODO3921)
0.4



CC Margin (ODO3921)
2.5



CC from Partial Hepatectomy (ODO4309)
0.2



Mets



Liver Margin (ODO4309)
1.8



Colon mets to lung (OD04451-01)
0.1



Lung Margin (OD04451-02)
0.1



Normal Prostate 6546-1
0.9



Prostate Cancer (OD04410)
1.2



Prostate Margin (OD04410)
3.2



Prostate Cancer (OD04720-01)
0.8



Prostate Margin (OD04720-02)
1.9



Normal Lung 061010
1.4



Lung Met to Muscle (ODO4286)
0.5



Muscle Margin (ODO4286)
16.4



Lung Malignant Cancer (OD03126)
0.1



Lung Margin (OD03126)
0.4



Lung Cancer (OD04404)
0.3



Lung Margin (OD04404)
0.3



Lung Cancer (OD04565)
0.0



Lung Margin (OD04565)
0.2



Lung Cancer (OD04237-01)
0.0



Lung Margin (OD04237-02)
0.1



Ocular Mel Met to Liver (ODO4310)
0.4



Liver Margin (ODO4310)
2.6



Melanoma Mets to Lung (OD04321)
0.0



Lung Margin (OD04321)
0.2



Normal Kidney
3.1



Kidney Ca, Nuclear grade 2 (OD04338)
0.0



Kidney Margin (OD04338)
0.0



Kidney Ca Nuclear grade 1/2 (OD04339)
0.2



Kidney Margin (OD04339)
1.1



Kidney Ca, Clear cell type (OD04340)
0.3



Kidney Margin (OD04340)
1.8



Kidney Ca, Nuclear grade 3 (OD04348)
0.0



Kidney Margin (OD04348)
1.3



Kidney Cancer (OD04622-01)
0.0



Kidney Margin (OD04622-03)
0.0



Kidney Cancer (OD04450-01)
0.1



Kidney Margin (OD04450-03)
1.2



Kidney Cancer 8120607
0.1



Kidney Margin 8120608
0.2



Kidney Cancer 8120613
0.7



Kidney Margin 8120614
2.0



Kidney Cancer 9010320
8.4



Kidney Margin 9010321
2.0



Normal Uterus
2.4



Uterus Cancer 064011
4.4



Normal Thyroid
2.3



Thyroid Cancer 064010
0.0



Thyroid Cancer A302152
0.4



Thyroid Margin A302153
0.5



Normal Breast
80.7



Breast Cancer (OD04566)
1.5



Breast Cancer (OD04590-01)
21.0



Breast Cancer Mets (OD04590-03)
100.0



Breast Cancer Metastasis (OD04655-05)
13.3



Breast Cancer 064006
0.9



Breast Cancer 1024
5.8



Breast Cancer 9100266
3.2



Breast Margin 9100265
5.9



Breast Cancer A209073
2.0



Breast Margin A209073
7.9



Normal Liver
3.3



Liver Cancer 064003
2.2



Liver Cancer 1025
6.0



Liver Cancer 1026
2.0



Liver Cancer 6004-T
7.5



Liver Tissue 6004-N
2.2



Liver Cancer 6005-T
1.8



Liver Tissue 6005-N
0.3



Normal Bladder
6.0



Bladder Cancer 1023
0.7



Bladder Cancer A302173
0.4



Bladder Cancer (OD04718-01)
0.1



Bladder Normal Adjacent (OD04718-03)
11.7



Normal Ovary
0.8



Ovarian Cancer 064008
1.1



Ovarian Cancer (OD04768-07)
0.7



Ovary Margin (OD04768-08)
2.9



Normal Stomach
15.1



Gastric Cancer 9060358
6.4



Stomach Margin 9060359
2.0



Gastric Cancer 9060395
8.8



Stomach Margin 9060394
8.4



Gastric Cancer 9060397
0.9



Stomach Margin 9060396
3.1



Gastric Cancer 064005
5.3









Column A - Rel. Exp.(%) Ag2840, Run 161922469














TABLE AJE










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
22.1



TE671- Medulloblastoma
10.1



D283 Med- Medulloblastoma
2.3



PFSK-1- Primitive Neuroectodermal
6.2



XF-498- CNS
8.4



SNB-78- Glioma
5.0



SF-268- Glioblastoma
1.4



T98G- Glioblastoma
0.0



SK-N-SH- Neuroblastoma (metastasis)
2.7



SF-295- Glioblastoma
2.9



Cerebellum
3.4



Cerebellum
38.2



NCI-H292- Mucoepidermoid lung
0.0



carcinoma



DMS-114- Small cell lung cancer
0.0



DMS-79- Small cell lung cancer
100.0



NCI-H146- Small cell lung cancer
0.0



NCI-H526- Small cell lung cancer
9.6



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
3.3



NCI-H157- Squamous cell lung cancer
0.0



(metastasis)



NCI-H1155- Large cell lung cancer
20.6



NCI-H1299- Large cell lung cancer
9.2



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
0.0



LX-1- Small cell lung cancer
7.9



Colo-205- Colon cancer
0.0



KM12- Colon cancer
0.0



KM20L2- Colon cancer
2.9



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
3.8



NCI-SNU-5- Gastric carcinoma
2.1



KATO III- Gastric carcinoma
12.0



NCI-SNU-16- Gastric carcinoma
5.8



NCI-SNU-1- Gastric carcinoma
2.6



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
7.2



MKN-45- Gastric carcinoma
96.6



NCI-N87- Gastric carcinoma
10.0



OVCAR-5- Ovarian carcinoma
3.7



RL95-2- Uterine carcinoma
7.2



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma
7.6



(metastasis)



ES-2- Ovarian clear cell carcinoma
0.0



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
0.0



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
10.4



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
5.9



JM1- pre-B-cell lymphoma
7.8



Jurkat- T cell leukemia
2.7



TF-1- Erythroleukemia
5.0



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
11.0



KU-812- Myelogenous leukemia
31.0



769-P- Clear cell renal carcinoma
6.1



Caki-2- Clear cell renal carcinoma
3.0



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
3.7



Hs766T- Pancreatic carcinoma (LN
9.8



metastasis)



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver
5.0



metastasis)



BxPC-3- Pancreatic adenocarcinoma
13.0



HPAC- Pancreatic adenocarcinoma
20.7



MIA PaCa-2- Pancreatic carcinoma
4.2



CFPAC-1- Pancreatic ductal adenocarcinoma
13.9



PANC-1- Pancreatic epithelioid ductal
69.7



carcinoma



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
0.0



HT-1197- Bladder carcinoma
6.4



UM-UC-3- Bladder carcinma (transitional cell)
2.4



A204- Rhabdomyosarcoma
12.5



HT-1080- Fibrosarcoma
12.1



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
16.7



SJRH30- Rhabdomyosarcoma (met to bone
5.7



marrow)



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
0.0



DU 145- Prostate carcinoma (brain
0.0



metastasis)



MDA-MB-468- Breast adenocarcinoma
11.4



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp.(%) Ag2840, Run 170190088














TABLE AJF










Panel 4.1D










Tissue Name
A














Secondary Th1 act
6.4



Secondary Th2 act
2.0



Secondary Tr1 act
4.0



Secondary Th1 rest
4.4



Secondary Th2 rest
13.9



Secondary Tr1 rest
8.2



Primary Th1 act
3.0



Primary Th2 act
9.6



Primary Tr1 act
1.6



Primary Th1 rest
3.2



Primary Th2 rest
5.6



Primary Tr1 rest
6.4



CD45RA CD4 lymphocyte act
6.0



CD45RO CD4 lymphocyte act
6.2



CD8 lymphocyte act
6.4



Secondary CD8 lymphocyte rest
2.4



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
12.5



2ry Th1/Th2/Tr1_anti-CD95 CH11
11.3



LAK cells rest
7.6



LAK cells IL-2
4.2



LAK cells IL-2 + IL-12
1.9



LAK cells IL-2 + IFN gamma
2.7



LAK cells IL-2 + IL-18
6.6



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
3.4



Two Way MLR 3 day
6.7



Two Way MLR 5 day
5.8



Two Way MLR 7 day
8.5



PBMC rest
9.3



PBMC PWM
1.0



PBMC PHA-L
3.2



Ramos (B cell) none
1.9



Ramos (B cell) ionomycin
3.0



B lymphocytes PWM
1.0



B lymphocytes CD40L and IL-4
9.4



EOL-1 dbcAMP
0.8



EOL-1 dbcAMP PMA/ionomycin
6.0



Dendritic cells none
0.9



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
3.1



Monocytes rest
13.8



Monocytes LPS
4.9



Macrophages rest
2.1



Macrophages LPS
0.9



HUVEC none
6.3



HUVEC starved
7.9



HUVEC IL-1beta
0.9



HUVEC IFN gamma
4.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
5.4



HUVEC IL-11
3.8



Lung Microvascular EC none
8.4



Lung Microvascular EC TNFalpha + IL-1beta
4.0



Microvascular Dermal EC none
4.5



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
10.4



Small airway epithelium none
5.9



Small airway epithelium TNFalpha + IL-1beta
8.5



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
1.0



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
1.4



KU-812 (Basophil) rest
8.4



KU-812 (Basophil) PMA/ionomycin
6.1



CCD1106 (Keratinocytes) none
6.7



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
1.8



Liver cirrhosis
63.3



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
3.3



Lung fibroblast none
29.5



Lung fibroblast TNF alpha + IL-1 beta
8.2



Lung fibroblast IL-4
7.9



Lung fibroblast IL-9
10.3



Lung fibroblast IL-13
4.2



Lung fibroblast IFN gamma
9.1



Dermal fibroblast CCD1070 rest
2.3



Dermal fibroblast CCD1070 TNF alpha
9.2



Dermal fibroblast CCD1070 IL-1beta
4.9



Dermal fibroblast IFN gamma
1.0



Dermal fibroblast IL-4
8.1



Dermal Fibroblasts rest
4.2



Neutrophils TNFa + LPS
25.0



Neutrophils rest
66.9



Colon
100.0



Lung
3.8



Thymus
24.5



Kidney
32.1









Column A - Rel. Exp.(%) Ag2840, Run 204964146














TABLE AJG










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
100.0



97476_Patient-07sk_skeletal muscle
22.8



97477_Patient-07ut_uterus
13.8



97478_Patient-07pl_placenta
0.0



99167_Bayer Patient 1
1.0



97482_Patient-08ut_uterus
6.1



97483_Patient-08pl_placenta
0.0



97486_Patient-09sk_skeletal muscle
2.3



97487_Patient-09ut_uterus
5.5



97488_Patient-09pl_placenta
0.0



97492_Patient-10ut_uterus
4.4



97493_Patient-10pl_placenta
0.0



97495_Patient-11go_adipose
24.3



97496_Patient-11sk_skeletal muscle
13.0



97497_Patient-11ut_uterus
11.7



97498_Patient-11pl_placenta
0.0



97500_Patient-12go_adipose
78.5



97501_Patient-12sk_skeletal muscle
63.3



97502_Patient-12ut_uterus
13.0



97503_Patient-12pl_placenta
0.0



94721_Donor 2 U - A_Mesenchymal
0.0



Stem Cells



94722_Donor 2 U - B_Mesenchymal
0.0



Stem Cells



94723_Donor 2 U - C_Mesenchymal
0.0



Stem Cells



94709_Donor 2 AM - A_adipose
18.9



94710_Donor 2 AM - B_adipose
12.5



94711_Donor 2 AM - C_adipose
15.2



94712_Donor 2 AD - A_adipose
25.2



94713_Donor 2 AD - B_adipose
30.4



94714_Donor 2 AD - C_adipose
42.3



94742_Donor 3 U - A_Mesenchymal Stem
0.0



Cells



94743_Donor 3 U - B_Mesenchymal Stem
0.0



Cells



94730_Donor 3 AM - A_adipose
12.6



94731_Donor 3 AM - B_adipose
7.9



94732_Donor 3 AM - C_adipose
11.0



94733_Donor 3 AD - A_adipose
26.8



94734_Donor 3 AD - B_adipose
18.0



94735_Donor 3 AD - C_adipose
17.1



77138_Liver_HepG2untreated
0.3



73556_Heart_Cardiac stromal cells
0.0



(primary)



81735_Small Intestine
12.9



72409_Kidney_Proximal Convoluted Tubule
0.0



82685_Small intestine_Duodenum
1.0



90650_Adrenal_Adrenocortical adenoma
0.0



72410_Kidney_HRCE
0.0



72411_Kidney_HRE
0.0



73139_Uterus_Uterine smooth muscle cells
0.0









Column A - Rel. Exp.(%) Ag6179, Run 256791278







General_screening_panel_v1.5 Summary: Ag6179 Highest expression of this gene was detected in skeletal muscle (CT=28.6). Moderate levels of expression of this gene are also seen in adipose, colon, small intestine and heart. This gene codes for a homolog of mouse S3-12 protein. S3-12 is a purported adipose cell membrane-associated protein that is upregulated during adipocyte differentiation. Due to its weak homology with adipophilins, this gene is involved in lipid uptake. Inhibiting its action by an antibody is useful for reducing the potential white adipose mass by limiting lipid uptake and inhibiting adipocyte differentiation. Its expression in skeletal muscle shows that it can function similarly in muscle tissue as well, in terms of lipid uptake. Since excess lipid storage in muscle is associated with insulin resistance, antibody inhibition of this gene product is useful as a treatment for the prevention of obesity-associated insulin resistance.


Furthermore, this gene product was also moderately expressed in a variety of metabolic tissues, including adrenal gland, heart, colon and small intestine. Thus, modulation of this gene or expressed protein is useful as marker for the treatment of metabolic disease, including Types 1 and 2 diabetes, and obesity.


In addition, moderate but significant expression in the spinal cordshows that the protein encoded by this gene plays a role in lipid processing in the central nervous system. LDLR has been implicated in the development of Alzheimer's disease. Therefore, modulation of this gene or gene product is useful in the treatment of Alzheimer's Disease.


Panel 2D Summary: Ag2840 This gene was moderately expressed in all tissue samples this panel, with highest expression in a metastatic breast cancer sample (CT=24.5). This gene showed higher expression in normal kidney, prostate, colon and bladder samples relative to the corresponding adjacent tumor sample. Expression of this gene is useful as a marker to differentiate between normal and cancerous tissues. Therapeutic modulation of this gene, expressed protein and/or use of agonists targeting this gene or gene product is useful in the treatment of kidney, prostate, colon and bladder cancers.


Panel 3D Summary: Ag2840 This gene showed low levels of expression in cancer cell lines, with highest expression in a gastric cancer MKN-45 cell line (CT=32). Significant expression was seen in cancer cell lines derived from lung cancer, pancreatic cancer and a leukemia. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drug targeting this gene or gene product is useful in the treatment of these cancers.


Panel 4.1D Summary: Ag2840 This transcript is expressed in colon and in resting neutrophils (CTs=31-33). Thus, the transcript or the protein it encodes is useful as a marker to detect colon tissue and neutrophils. Therapeutics modulation of this gene, expressed protein and/or use of antibodies or small molecule targeting this gene or gene product are useful in the treatment of autoimmune and inflammatory diseases such as asthma, allergies, inflammatory bowel disease including Crohn's and Colitis, lupus erythematosus, psoriasis, rheumatoid arthritis, and osteoarthritis.


Panel 5 Islet Summary: Ag6179 Highest expression of this gene was detected in adipose tissue from a diabetic patient. Significant expression of this gene was also seen in midway and fully differentiated adipose tissue. This gene was moderately expressed in clinical specimens of adipose, skeletal muscle and uterus.


AK. CG5590602 and CG5590604 and CG5590605: Human Ortholog of Mouse S3-12


Expression of full-length physical clone CG55906-02 and variants CG55906-04 and CG55906-05 was assessed using the primer-probe sets Ag2840, Ag5976 and Ag6345, described in Tables AKA, AKB and AKC. Results of the RTQ-PCR runs are shown in Tables AKD, AKE, AKF, AKG and AKH. Please note that only the probe and primer set Ag6345 corresponds to CG55906-02 and only probe and primer sets Ag5976 and Ag6345 correspond to CG55906-05.

TABLE AKAProbe Name Ag2840StartSEQ IDPrimersSequencesLengthPositionNoForward5′-tctatggtcatgggtacgaaag-3′2211831450ProbeTET-5′-acacgatgtccactgggctcacag-3′-2412051451TAMRAReverse5′-gttgtgttcagcccagtttg-3′2012581452









TABLE AKB










Probe Name Ag5976















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-caacttcggaaaagcagat-3′
20
244
1453






Probe
TET-5′-ctggacatcttggaacacaccaggtc-3′-
26
280
1454



TAMRA





Reverse
5′-ggaggacacggcatcct-3′
17
311
1455
















TABLE AKC










Probe Name Ag6345















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-aacgcacatagctcggcga-3′
19
112
1456






Probe
TET-5′-tgaccccacaggagcgcctgct-3′-TAMRA
22
147
1457





Reverse
5′-aaggttgcagctccttctccgt-3′
22
229
1458
















TABLE AKD










General_screening_panel_v1.5











Tissue Name
A
B















Adipose
100.0
0.0



Melanoma* Hs688(A).T
0.0
0.0



Melanoma* Hs688(B).T
0.0
0.0



Melanoma* M14
0.0
0.0



Melanoma* LOXIMVI
0.0
0.0



Melanoma* SK-MEL-5
0.0
0.0



Squamous cell carcinoma SCC-4
0.0
0.0



Testis Pool
0.6
1.6



Prostate ca.* (bone met) PC-3
3.2
3.6



Prostate Pool
1.4
1.3



Placenta
0.0
0.0



Uterus Pool
2.8
0.9



Ovarian ca. OVCAR-3
0.0
3.0



Ovarian ca. SK-OV-3
0.0
0.0



Ovarian ca. OVCAR-4
0.1
0.0



Ovarian ca. OVCAR-5
4.3
7.9



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca. OVCAR-8
0.0
0.0



Ovary
0.5
0.7



Breast ca. MCF-7
0.3
0.0



Breast ca. MDA-MB-231
0.0
0.0



Breast ca. BT 549
0.0
0.0



Breast ca. T47D
0.0
0.0



Breast ca. MDA-N
0.0
0.0



Breast Pool
8.2
6.7



Trachea
7.3
1.5



Lung
0.3
0.0



Fetal Lung
0.4
0.9



Lung ca. NCI-N417
0.0
0.0



Lung ca. LX-1
0.1
0.0



Lung ca. NCI-H146
0.0
0.0



Lung ca. SHP-77
0.0
0.0



Lung ca. A549
0.0
0.0



Lung ca. NCI-H526
0.0
0.0



Lung ca. NCI-H23
0.0
0.0



Lung ca. NCI-H460
0.0
0.0



Lung ca. HOP-62
0.0
0.0



Lung ca. NCI-H522
0.0
0.0



Liver
0.7
0.0



Fetal Liver
1.2
1.7



Liver ca. HepG2
2.0
1.2



Kidney Pool
15.5
41.8



Fetal Kidney
0.1
0.0



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.3
0.0



Bladder
3.4
0.0



Gastric ca. (liver met.) NCI-N87
2.2
3.9



Gastric ca. KATO III
0.1
1.7



Colon ca. SW-948
0.0
0.0



Colon ca. SW480
0.0
0.0



Colon ca.* (SW480 met) SW620
0.0
0.0



Colon ca. HT29
1.9
1.4



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
0.3
0.0



Colon cancer tissue
15.4
15.5



Colon ca. SW1116
0.0
0.0



Colon ca. Colo-205
0.0
0.0



Colon ca. SW-48
0.0
0.0



Colon Pool
9.4
7.0



Small Intestine Pool
4.8
1.4



Stomach Pool
6.1
6.0



Bone Marrow Pool
3.8
2.6



Fetal Heart
0.2
0.0



Heart Pool
12.2
5.0



Lymph Node Pool
10.4
13.9



Fetal Skeletal Muscle
2.0
0.0



Skeletal Muscle Pool
54.7
100.0



Spleen Pool
0.0
0.0



Thymus Pool
6.2
5.5



CNS cancer (glio/astro) U87-MG
0.0
0.0



CNS cancer (glio/astro) U-118-MG
0.0
0.0



CNS cancer (neuro; met) SK-N-AS
0.0
0.3



CNS cancer (astro) SF-539
0.0
0.0



CNS cancer (astro) SNB-75
0.0
0.0



CNS cancer (glio) SNB-19
0.0
0.0



CNS cancer (glio) SF-295
0.5
0.0



Brain (Amygdala) Pool
0.5
2.7



Brain (cerebellum)
0.4
0.0



Brain (fetal)
0.3
0.0



Brain (Hippocampus) Pool
0.0
2.4



Cerebral Cortex Pool
1.0
1.1



Brain (Substantia nigra) Pool
0.6
0.9



Brain (Thalamus) Pool
0.9
0.0



Brain (whole)
0.4
0.0



Spinal Cord Pool
2.5
2.9



Adrenal Gland
4.2
1.6



Pituitary gland Pool
0.4
0.0



Salivary Gland
3.0
3.1



Thyroid (female)
0.6
0.0



Pancreatic ca. CAPAN2
1.6
0.6



Pancreas Pool
8.5
18.2









Column A - Rel. Exp. (%) Ag5976, Run 248204740






Column B - Rel. Exp. (%) Ag6345, Run 259476291














TABLE AKE










Panel 2D










Tissue Name
A














Normal Colon
41.8



CC Well to Mod Diff (ODO3866)
4.3



CC Margin (ODO3866)
6.9



CC Gr.2 rectosigmoid (ODO3868)
1.1



CC Margin (ODO3868)
4.5



CC Mod Diff (ODO3920)
0.0



CC Margin (ODO3920)
0.0



CC Gr.2 ascend colon (ODO3921)
0.4



CC Margin (ODO3921)
2.5



CC from Partial Hepatectomy (ODO4309) Mets
0.2



Liver Margin (ODO4309)
1.8



Colon mets to lung (OD04451-01)
0.1



Lung Margin (OD04451-02)
0.1



Normal Prostate 6546-1
0.9



Prostate Cancer (OD04410)
1.2



Prostate Margin (OD04410)
3.2



Prostate Cancer (OD04720-01)
0.8



Prostate Margin (OD04720-02)
1.9



Normal Lung 061010
1.4



Lung Met to Muscle (ODO4286)
0.5



Muscle Margin (ODO4286)
16.4



Lung Malignant Cancer (OD03126)
0.1



Lung Margin (OD03126)
0.4



Lung Cancer (OD04404)
0.3



Lung Margin (OD04404)
0.3



Lung Cancer (OD04565)
0.0



Lung Margin (OD04565)
0.2



Lung Cancer (OD04237-01)
0.0



Lung Margin (OD04237-02)
0.1



Ocular Mel Met to Liver (ODO4310)
0.4



Liver Margin (ODO4310)
2.6



Melanoma Mets to Lung (OD04321)
0.0



Lung Margin (OD04321)
0.2



Normal Kidney
3.1



Kidney Ca, Nuclear grade 2 (OD04338)
0.0



Kidney Margin (OD04338)
0.0



Kidney Ca Nuclear grade 1/2 (OD04339)
0.2



Kidney Margin (OD04339)
1.1



Kidney Ca, Clear cell type (OD04340)
0.3



Kidney Margin (OD04340)
1.8



Kidney Ca, Nuclear grade 3 (OD04348)
0.0



Kidney Margin (OD04348)
1.3



Kidney Cancer (OD04622-01)
0.0



Kidney Margin (OD04622-03)
0.0



Kidney Cancer (OD04450-01)
0.1



Kidney Margin (OD04450-03)
1.2



Kidney Cancer 8120607
0.10



Kidney Margin 8120608
0.2



Kidney Cancer 8120613
0.7



Kidney Margin 8120614
2.0



Kidney Cancer 9010320
8.4



Kidney Margin 9010321
2.0



Normal Uterus
2.4



Uterus Cancer 064011
4.4



Normal Thyroid
2.3



Thyroid Cancer 064010
0.0



Thyroid Cancer A302152
0.4



Thyroid Margin A302153
0.5



Normal Breast
80.7



Breast Cancer (OD04566)
1.5



Breast Cancer (OD04590-01)
21.0



Breast Cancer Mets (OD04590-03)
100.0



Breast Cancer Metastasis (OD04655-05)
13.3



Breast Cancer 064006
0.9



Breast Cancer 1024
5.8



Breast Cancer 9100266
3.2



Breast Margin 9100265
5.9



Breast Cancer A209073
2.0



Breast Margin A209073
7.9



Normal Liver
3.3



Liver Cancer 064003
2.2



Liver Cancer 1025
6.0



Liver Cancer 1026
2.0



Liver Cancer 6004-T
7.5



Liver Tissue 6004-N
2.2



Liver Cancer 6005-T
1.8



Liver Tissue 6005-N
0.3



Normal Bladder
6.0



Bladder Cancer 1023
0.7



Bladder Cancer A302173
0.4



Bladder Cancer (OD04718-01)
0.1



Bladder Normal Adjacent (OD04718-03)
11.7



Normal Ovary
0.8



Ovarian Cancer 064008
1.1



Ovarian Cancer (OD04768-07)
0.7



Ovary Margin (OD04768-08)
2.9



Normal Stomach
15.1



Gastric Cancer 9060358
6.4



Stomach Margin 9060359
2.0



Gastric Cancer 9060395
8.8



Stomach Margin 9060394
8.4



Gastric Cancer 9060397
0.9



Stomach Margin 9060396
3.1



Gastric Cancer 064005
5.3









Column A - Rel. Exp. (%) Ag2840, Run 161922469














TABLE AKF










Panel 3D








Tissue Name
A











Daoy- Medulloblastoma
22.1


TE671- Medulloblastoma
10.1


D283 Med- Medulloblastoma
2.3


PFSK-1- Primitive Neuroectodermal
6.2


XF-498- CNS
8.4


SNB-78- Glioma
5.0


SF-268- Glioblastoma
1.4


T98G- Glioblastoma
0.0


SK-N-SH- Neuroblastoma (metastasis)
2.7


SF-295- Glioblastoma
2.9


Cerebellum
3.4


Cerebellum
38.2


NCI-H292- Mucoepidermoid lung carcinoma
0.0


DMS-114- Small cell lung cancer
0.0


DMS-79- Small cell lung cancer
100.0


NCI-H146- Small cell lung cancer
0.0


NCI-H526- Small cell lung cancer
9.6


NCI-N417- Small cell lung cancer
0.0


NCI-H82- Small cell lung cancer
3.3


NCI-H157- Squamous cell lung cancer (metastasis)
0.0


NCI-H1155- Large cell lung cancer
20.6


NCI-H1299- Large cell lung cancer
9.2


NCI-H727- Lung carcinoid
0.0


NCI-UMC-11- Lung carcinoid
0.0


LX-1- Small cell lung cancer
7.9


Colo-205- Colon cancer
0.0


KM12- Colon cancer
0.0


KM20L2- Colon cancer
2.9


NCI-H716- Colon cancer
0.0


SW-48- Colon adenocarcinoma
0.0


SW1116- Colon adenocarcinoma
0.0


LS 174T- Colon adenocarcinoma
0.0


SW-948- Colon adenocarcinoma
0.0


SW-480- Colon adenocarcinoma
3.8


NCI-SNU-5- Gastric carcinoma
2.1


KATO III- Gastric carcinoma
12.0


NCI-SNU-16- Gastric carcinoma
5.8


NCI-SNU-1- Gastric carcinoma
2.6


RF-1- Gastric adenocarcinoma
0.0


RF-48- Gastric adenocarcinoma
7.2


MKN-45- Gastric carcinoma
96.6


NCI-N87- Gastric carcinoma
10.0


OVCAR-5- Ovarian carcinoma
3.7


RL95-2- Uterine carcinoma
7.2


HelaS3- Cervical adenocarcinoma
0.0


Ca Ski- Cervical epidermoid carcinoma (metastasis)
7.6


ES-2- Ovarian clear cell carcinoma
0.0


Ramos- Stimulated with PMA/ionomycin 6 h
0.0


Ramos- Stimulated with PMA/ionomycin 14 h
0.0


MEG-01- Chronic myelogenous leukemia (megokaryoblast)
0.0


Raji- Burkitt's lymphoma
0.0


Daudi- Burkitt's lymphoma
0.0


U266- B-cell plasmacytoma
10.4


CA46- Burkitt's lymphoma
0.0


RL- non-Hodgkin's B-cell lymphoma
5.9


JM1- pre-B-cell lymphoma
7.8


Jurkat- T cell leukemia
2.7


TF-1- Erythroleukemia
5.0


HUT 78- T-cell lymphoma
0.0


U937- Histiocytic lymphoma
11.0


KU-812- Myelogenous leukemia
31.0


769-P- Clear cell renal carcinoma
6.1


Caki-2- Clear cell renal carcinoma
3.0


SW 839- Clear cell renal carcinoma
0.0


Rhabdoid kidney tumor
3.7


Hs766T- Pancreatic carcinoma (LN metastasis)
9.8


CAPAN-1- Pancreatic adenocarcinoma (liver metastasis)
0.0


SU86.86- Pancreatic carcinoma (liver metastasis)
5.0


BxPC-3- Pancreatic adenocarcinoma
13.0


HPAC- Pancreatic adenocarcinoma
20.7


MIA PaCa-2- Pancreatic carcinoma
4.2


CFPAC-1- Pancreatic ductal adenocarcinoma
13.9


PANC-1- Pancreatic epithelioid ductal carcinoma
69.7


T24- Bladder carcinma (transitional cell)
0.0


5637- Bladder carcinoma
0.0


HT-1197- Bladder carcinoma
6.4


UM-UC-3- Bladder carcinma (transitional cell)
2.4


A204- Rhabdomyosarcoma
12.5


HT-1080- Fibrosarcoma
12.1


MG-63- Osteosarcoma
0.0


SK-LMS-1- Leiomyosarcoma (vulva)
16.7


SJRH30- Rhabdomyosarcoma (met to bone marrow)
5.7


A431- Epidermoid carcinoma
0.0


WM266-4- Melanoma
0.0


DU 145- Prostate carcinoma (brain metastasis)
0.0


MDA-MB-468- Breast adenocarcinoma
11.4


SCC-4- Squamous cell carcinoma of tongue
0.0


SCC-9- Squamous cell carcinoma of tongue
0.0


SCC-15- Squamous cell carcinoma of tongue
0.0


CAL 27- Squamous cell carcinoma of tongue
0.0







Column A - Rel. Exp. (%) Ag2840, Run 170190088














TABLE AKG










Panel 4.1D









Tissue Name
A
B












Secondary Th1 act
6.4
0.0


Secondary Th2 act
2.0
0.0


Secondary Tr1 act
4.0
0.0


Secondary Th1 rest
4.4
0.0


Secondary Th2 rest
13.9
0.0


Secondary Tr1 rest
8.2
0.0


Primary Th1 act
3.0
0.0


Primary Th2 act
9.6
0.0


Primary Tr1 act
1.6
0.0


Primary Th1 rest
3.2
0.0


Primary Th2 rest
5.6
0.0


Primary Tr1 rest
6.4
0.0


CD45RA CD4 lymphocyte act
6.0
0.0


CD45RO CD4 lymphocyte act
6.2
0.0


CD8 lymphocyte act
6.4
0.0


Secondary CD8 lymphocyte rest
2.4
0.0


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
12.5
0.0


2ry Th1/Th2/Tr1_anti-CD95 CH11
11.3
0.0


LAK cells rest
7.6
0.0


LAK cells IL-2
4.2
0.0


LAK cells IL-2 + IL-12
1.9
0.0


LAK cells IL-2 + IFN gamma
2.7
0.0


LAK cells IL-2 + IL-18
6.6
0.0


LAK cells PMA/ionomycin
0.0
0.0


NK Cells IL-2 rest
3.4
0.0


Two Way MLR 3 day
6.7
0.0


Two Way MLR 5 day
5.8
0.0


Two Way MLR 7 day
8.5
0.0


PBMC rest
9.3
0.0


PBMC PWM
1.0
0.0


PBMC PHA-L
3.2
0.0


Ramos (B cell) none
1.9
0.0


Ramos (B cell) ionomycin
3.0
0.0


B lymphocytes PWM
1.0
0.0


B lymphocytes CD40L and IL-4
9.4
0.0


EOL-1 dbcAMP
0.8
0.0


EOL-1 dbcAMP PMA/ionomycin
6.0
0.0


Dendritic cells none
0.9
0.0


Dendritic cells LPS
0.0
0.0


Dendritic cells anti-CD40
3.1
0.0


Monocytes rest
13.8
0.0


Monocytes LPS
4.9
0.0


Macrophages rest
2.1
0.0


Macrophages LPS
0.9
0.0


HUVEC none
6.3
0.0


HUVEC starved
7.9
0.0


HUVEC IL-1beta
0.9
0.0


HUVEC IFN gamma
4.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
5.4
0.0


HUVEC IL-11
3.8
0.0


Lung Microvascular EC none
8.4
0.0


Lung Microvascular EC TNFalpha + IL-1beta
4.0
0.0


Microvascular Dermal EC none
4.5
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.0


Bronchial epithelium TNFalpha + IL1beta
10.4
0.0


Small airway epithelium none
5.9
0.0


Small airway epithelium TNFalpha + IL-1beta
8.5
0.0


Coronery artery SMC rest
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
1.0
0.0


Astrocytes rest
0.0
0.0


Astrocytes TNFalpha + IL-1beta
1.4
0.0


KU-812 (Basophil) rest
8.4
20.0


KU-812 (Basophil) PMA/ionomycin
6.1
0.0


CCD1106 (Keratinocytes) none
6.7
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
1.8
0.0


Liver cirrhosis
63.3
0.0


NCI-H292 none
0.0
0.0


NCI-H292 IL-4
0.0
56.3


NCI-H292 IL-9
0.0
65.5


NCI-H292 IL-13
0.0
48.6


NCI-H292 IFN gamma
0.0
24.3


HPAEC none
0.0
0.0


HPAEC TNF alpha + IL-1 beta
3.3
0.0


Lung fibroblast none
29.5
100.0


Lung fibroblast TNF alpha + IL-1 beta
8.2
0.0


Lung fibroblast IL-4
7.9
0.0


Lung fibroblast IL-9
10.3
0.0


Lung fibroblast IL-13
4.2
0.0


Lung fibroblast IFN gamma
9.1
0.0


Dermal fibroblast CCD1070 rest
2.3
0.0


Dermal fibroblast CCD1070 TNF alpha
9.2
0.0


Dermal fibroblast CCD1070 IL-1 beta
4.9
0.0


Dermal fibroblast IFN gamma
1.0
0.0


Dermal fibroblast IL-4
8.1
0.0


Dermal Fibroblasts rest
4.2
0.0


Neutrophils TNFa + LPS
25.0
0.0


Neutrophils rest
66.9
18.7


Colon
100.0
54.7


Lung
3.8
0.0


Thymus
24.5
46.7


Kidney
32.1
0.0







Column A - Rel. Exp. (%) Ag2840, Run 204964146





Column B - Rel. Exp. (%) Ag5976, Run 248122612














TABLE AKH










Panel 5D










Tissue Name
A














97457_Patient-02go_adipose
63.3



97476_Patient-07sk_skeletal muscle
15.8



97477_Patient-07ut_uterus
8.8



97478_Patient-07pl_placenta
0.0



97481_Patient-08sk_skeletal muscle
15.7



97482_Patient-08ut_uterus
8.4



97483_Patient-08pl_placenta
0.0



97486_Patient-09sk_skeletal muscle
1.4



97487_Patient-09ut_uterus
5.5



97488_Patient-09pl_placenta
0.0



97492_Patient-10ut_uterus
4.6



97493_Patient-10pl_placenta
0.3



97495_Patient-11go_adipose
0.0



97496_Patient-11sk_skeletal muscle
0.0



97497_Patient-11ut_uterus
0.0



97498_Patient-11pl_placenta
0.0



97500_Patient-12go_adipose
48.0



97501_Patient-12sk_skeletal muscle
100.0



97502_Patient-12ut_uterus
18.7



97503_Patient-12pl_placenta
0.0



94721_Donor 2 U - A_Mesenchymal Stem Cells
0.0



94722_Donor 2 U - B_Mesenchymal Stem Cells
0.2



94723_Donor 2 U - C_Mesenchymal Stem Cells
0.0



94709_Donor 2 AM - A_adipose
24.1



94710_Donor 2 AM - B_adipose
7.9



94711_Donor 2 AM - C_adipose
4.5



94712_Donor 2 AD - A_adipose
18.3



94713_Donor 2 AD - B_adipose
24.1



94714_Donor 2 AD - C_adipose
23.5



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
0.0



94730_Donor 3 AM - A_adipose
0.0



94731_Donor 3 AM - B_adipose
0.0



94732_Donor 3 AM - C_adipose
0.1



94733_Donor 3 AD - A_adipose
0.0



94734_Donor 3 AD - B_adipose
0.0



94735_Donor 3 AD - C_adipose
0.0



77138_Liver_HepG2untreated
1.1



73556_Heart_Cardiac stromal cells (primary)
0.0



81735_Small Intestine
16.8



72409_Kidney_Proximal Convoluted Tubule
0.0



82685_Small intestine_Duodenum
0.7



90650_Adrenal_Adrenocortical adenoma
0.1



72410_Kidney_HRCE
0.0



72411_Kidney_HRE
0.0



73139_Uterus_Uterine smooth muscle cells
0.2









Column A - Rel. Exp. (%) Ag2840, Run 169270970







General_screening_panel_v1.5 Summary: Ag5976 Highest expression of this gene was detected in adipose (CT=29). Moderate levels of expression of this gene was also seen in skeletal muscle, colon, small intestine, adrenal gland and heart. This gene codes for a homolog of mouse S3-12 protein. S3-12 is an adipose cell membrane-associated protein upregulated during adipocyte differentiation. Due to its weak homology with adipophilins, it is involved in lipid uptake. Inhibiting its action by an antibody will reduce potential white adipose mass by limiting lipid uptake and thereby inhibit adipocyte differentiation. Its expression in skeletal muscle indicates that it functions similarly in muscle tissue as well, in terms of lipid uptake. Since excess lipid storage in muscle is associated with insulin resistance, antibody inhibition of this gene product is useful as a treatment for the prevention of obesity-associated insulin resistance.


Furthermore, modulation of this gene or expressed protein is useful for the treatment of metabolic disease, including Types 1 and 2 diabetes, and obesity.


Low expression of this gene was also seen in colon cancer tissue and cancer cell lines derived from ovarian and prostate cancers.


Low levels of expression in the spinal cord show that the protein encoded by this gene plays a role in lipid processing in the central nervous system. Modulation of this gene or gene product is useful in the treatment of spinal cord related disorders.


Ag6345 Highest expression of this gene was detected in adipose and skeletal muscle (CTs=31). Significant expression of this gene was also seen in pancreas, and kidney.


Panel 2D Summary: Ag2840 This gene was moderately expressed in all tissue samples this panel, with highest expression in metastatic breast cancer (CT=24.5). This gene showed higher expression in normal kidney, prostate, colon and bladder samples relative to the corresponding adjacent tumor sample. Expression of this gene is useful to differentiate between normal and cancerous tissues. Therapeutic modulation of this gene, expressed protein and/or use of agonists targeting this gene or gene product is useful in the treatment of kidney, prostate, colon and bladder cancers.


Panel 3D Summary: Ag2840 This gene showed low levels of expression in the cancer cell lines, with highest expression in the gastric cancer MKN-45 cell line (CT=32). Significant expression was seen in cancer cell lines derived from lung cancer, pancreatic cancer and a leukemia. Therapeutic modulation of this gene, expressed protein and/or use of antibodies or small molecule drug targeting this gene or gene product is useful in the treatment of these cancers.


Panel 4.1D Summary: Ag2840 This transcript was expressed in colon and in resting neutrophils (CTs=31-33). The transcript or the protein it encodes are isefi; as markers to detect colon tissue and neutrophils. Therapeutics modulation of this gene, expressed protein and/or use of antibodies or small molecule targeting this gene or gene product is useful in the treatment of autoimmune and inflammatory diseases such as asthma, allergies, inflammatory bowel disease including Crohn's and Colitis, lupus erythematosus, psoriasis, rheumatoid arthritis, and osteoarthritis.


Panel 5D Summary: Ag2840 This gene was moderately expressed in clinical specimens of adipose, skeletal muscle and uterus. This confirms expression of this gene in tissues with metabolic function. Please see Panel 1.5D for discussion of utility of this gene in metabolic disease.


AL. CG5590801: Integrin A and FG GAP Domain Protein


Expression of full-length physical clone CG55908-01 was assessed using the primer-probe sets Ag6400, Ag6446, Ag6449 and Ag6964, described in Tables ALA, ALB, ALC and ALD. Results of the RTQ-PCR runs are shown in Table ALE.

TABLE ALAProbe Name Ag6400StartSEQ IDPrimersSequencesLengthPositionNoForward5′-gagctgatatgcaaaaggaaag-3′223001459ProbeTET-5′-ctgactcccaaccactggttctcctt-3′-263231460TAMRAReverse5′-gggcgcagtcaacaatct-3′183751461









TABLE ALB










Probe Name Ag6446















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-gcttcttccatcggagca-3′
18
726
1462






Probe
TET-5′-caactatcaccgggcctgtctggc-3′-
24
766
1463



TAMRA





Reverse
5′-catggctgaaggctgca-3′
17
792
1464
















TABLE ALC










Probe Name Ag6449















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-gagtcagtgttcggagcca-3′
19
342
1465






Probe
TET-5′-ctgcccactctacagctttgaccgc-3′-
25
421
1466



TAMRA





Reverse
5′-cccagacatgcagcacag-3′
18
450
1467
















TABLE ALD










Probe Name Ag6964















Start
SEQ ID



Primers
Sequences
Length
Position
No















Forward
5′-ggccccagacatgca-3′
15
456
1468






Probe
TET-5′-actctacagctttgacccgcgcgg-3′-TAMRA
23
427
1469





Reverse
5′-gccaactgtgtggtgttca-3′
19
401
1470
















TABLE ALE










General_screening_panel_v1.6











Tissue Name
A
B
C
D














Adipose
0.0
1.7
0.0
18.8


Melanoma* Hs688(A).T
0.0
0.1
0.0
0.7


Melanoma* Hs688(B).T
0.0
0.1
0.0
2.4


Melanoma* M14
0.0
0.1
0.0
0.7


Melanoma* LOXIMVI
0.0
0.1
0.0
0.1


Melanoma* SK-MEL-5
0.0
6.8
0.0
15.9


Squamous cell carcinoma SCC-4
0.0
0.0
0.0
0.1


Testis Pool
0.0
5.8
0.0
9.9


Prostate ca.* (bone met) PC-3
0.0
7.7
0.0
4.3


Prostate Pool
0.0
1.9
0.0
10.0


Placenta
0.0
0.9
0.0
0.4


Uterus Pool
0.0
0.3
0.0
4.1


Ovarian ca. OVCAR-3
0.0
4.8
0.0
4.0


Ovarian ca. SK-OV-3
0.0
2.5
0.0
1.7


Ovarian ca. OVCAR-4
0.0
0.5
0.0
0.5


Ovarian ca. OVCAR-5
0.0
15.6
0.0
7.9


Ovarian ca. IGROV-1
22.2
5.4
79.0
75.8


Ovarian ca. OVCAR-8
0.0
4.2
0.0
16.7


Ovary
0.0
0.2
0.0
2.4


Breast ca. MCF-7
0.0
0.9
0.0
0.5


Breast ca. MDA-MB-231
0.0
0.2
0.0
0.3


Breast ca. BT 549
0.0
0.2
0.0
0.4


Breast ca. T47D
0.0
0.7
0.0
0.5


Breast ca. MDA-N
0.0
0.0
10.2
0.8


Breast Pool
0.0
2.0
0.0
16.7


Trachea
0.0
0.5
0.0
5.6


Lung
0.0
0.5
0.0
5.1


Fetal Lung
0.0
0.5
0.0
6.1


Lung ca. NCI-N417
0.0
0.4
0.0
2.3


Lung ca. LX-1
0.0
100.0
0.0
44.1


Lung ca. NCI-H146
0.0
0.1
0.0
0.1


Lung ca. SHP-77
0.0
0.1
0.0
3.8


Lung ca. A549
0.0
14.3
0.0
4.7


Lung ca. NCI-H526
0.0
0.0
0.0
0.5


Lung ca. NCI-H23
0.0
15.9
0.0
10.3


Lung ca. NCI-H460
0.0
0.1
0.0
0.3


Lung ca. HOP-62
0.0
0.2
0.0
0.7


Lung ca. NCI-H522
0.0
27.7
0.0
8.9


Liver
0.0
5.3
0.0
2.0


Fetal Liver
0.0
23.0
0.0
8.2


Liver ca. HepG2
0.0
7.3
0.0
2.4


Kidney Pool
21.9
5.3
0.0
32.8


Fetal Kidney
0.0
20.2
0.0
11.5


Renal ca. 786-0
0.0
1.7
0.0
0.9


Renal ca. A498
0.0
23.0
0.0
8.5


Renal ca. ACHN
0.0
3.8
0.0
2.5


Renal ca. UO-31
0.0
0.7
0.0
0.3


Renal ca. TK-10
0.0
6.4
0.0
4.6


Bladder
0.0
3.2
0.0
6.7


Gastric ca. (liver met.) NCI-N87
0.0
17.8
0.0
6.7


Gastric ca. KATO III
0.0
1.3
0.0
0.9


Colon ca. SW-948
0.0
6.1
0.0
1.2


Colon ca. SW480
21.6
39.0
0.0
33.7


Colon ca.* (SW480 met) SW620
0.0
71.2
0.0
25.0


Colon ca. HT29
0.0
3.5
0.0
0.3


Colon ca. HCT-116
0.0
6.4
0.0
4.3


Colon ca. CaCo-2
12.2
78.5
0.0
38.2


Colon cancer tissue
0.0
21.9
0.0
20.4


Colon ca. SW1116
0.0
19.5
0.0
6.0


Colon ca. Colo-205
0.0
3.0
0.0
0.8


Colon ca. SW-48
0.0
4.2
0.0
2.6


Colon Pool
0.0
3.1
5.1
20.6


Small Intestine Pool
0.0
2.5
0.0
10.4


Stomach Pool
0.0
1.1
0.0
10.7


Bone Marrow Pool
0.0
1.1
0.0
12.5


Fetal Heart
0.0
2.7
0.0
20.7


Heart Pool
0.0
3.4
0.0
26.1


Lymph Node Pool
0.0
2.8
0.0
24.7


Fetal Skeletal Muscle
0.0
57.0
0.0
50.7


Skeletal Muscle Pool
0.0
24.3
0.0
32.3


Spleen Pool
0.0
2.6
0.0
3.1


Thymus Pool
0.0
1.4
0.0
7.0


CNS cancer (glio/astro) U87-MG
0.0
6.3
0.0
14.1


CNS cancer (glio/astro) U-118-MG
0.0
5.1
0.0
5.8


CNS cancer (neuro; met) SK-N-AS
0.0
3.9
0.0
2.6


CNS cancer (astro) SF-539
0.0
0.3
0.0
0.1


CNS cancer (astro) SNB-75
0.0
2.4
0.0
9.7


CNS cancer (glio) SNB-19
0.0
5.2
100.0
100.0


CNS cancer (glio) SF-295
0.0
14.9
0.0
14.8


Brain (Amygdala) Pool
0.0
1.1
0.0
5.3


Brain (cerebellum)
0.0
1.4
0.0
9.7


Brain (fetal)
0.0
1.1
0.0
6.4


Brain (Hippocampus) Pool
0.0
2.0
0.0
10.2


Cerebral Cortex Pool
0.0
2.0
0.0
8.7


Brain (Substantia nigra) Pool
0.0
1.1
0.0
9.3


Brain (Thalamus) Pool
0.0
3.2
0.0
8.7


Brain (whole)
0.0
1.9
0.0
8.7


Spinal Cord Pool
100.0
2.9
0.0
9.0


Adrenal Gland
0.0
0.7
0.0
4.1


Pituitary gland Pool
0.0
0.4
0.0
0.5


Salivary Gland
0.0
0.2
0.0
1.0


Thyroid (female)
0.0
0.8
0.0
2.3


Pancreatic ca. CAPAN2
0.0
4.6
0.0
2.2


Pancreas Pool
0.0
2.6
0.0
2.3







Column A - Rel. Exp. (%) Ag6400, Run 277248363





Column B - Rel. Exp. (%) Ag6446, Run 277250179





Column C - Rel. Exp. (%) Ag6449, Run 277250185





Column D - Rel. Exp. (%) Ag6964, Run 278388946







General_screening_panel_v1.6 Summary: Highest expression of this gene was detected in an ovarian cancer IGROV-1 cell line and a brain cancer SNB-19 cell line (CTs=25-33.7). Moderate to low levels of expression of this gene are also seen in all the regions of central nervous system, tissues with metabolic/endocrine functions, and number of cancer cell lines.


AM. CG56110-03: B7 H1


Expression of full-length physical clone CG56110-03 was assessed using the primer-probe set Ag1544, described in Table AMA. Results of the RTQ-PCR runs are shown in Tables AMB, AMC and AMD.

TABLE AMAProbe Name Ag1544SEQStartIDPrimersSequencesLengthPositionNoForward5′-tctggacaagcagtgaccat-3′204971471ProbeTET-5′-accaccaccaattccaagagagagga-3′-265381472TAMRAReverse5′-ttctcagtgtgctggtcaca-3′1205761473









TABLE AMB










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
3.6



Pancreas
2.9



Pancreatic ca. CAPAN 2
5.0



Adrenal gland
2.4



Thyroid
2.3



Salivary gland
1.7



Pituitary gland
6.3



Brain (fetal)
1.8



Brain (whole)
3.5



Brain (amygdala)
2.8



Brain (cerebellum)
1.7



Brain (hippocampus)
4.5



Brain (substantia nigra)
2.7



Brain (thalamus)
4.4



Cerebral Cortex
10.4



Spinal cord
2.0



glio/astro U87-MG
25.5



glio/astro U-118-MG
8.2



astrocytoma SW1783
7.9



neuro*; met SK-N-AS
9.2



astrocytoma SF-539
6.3



astrocytoma SNB-75
37.9



glioma SNB-19
12.3



glioma U251
14.7



glioma SF-295
6.0



Heart (fetal)
9.0



Heart
5.4



Skeletal muscle (fetal)
6.2



Skeletal muscle
1.9



Bone marrow
3.2



Thymus
13.4



Spleen
14.0



Lymph node
27.0



Colorectal
1.5



Stomach
3.4



Small intestine
2.7



Colon ca. SW480
2.2



Colon ca.* SW620(SW480 met)
0.8



Colon ca. HT29
1.6



Colon ca. HCT-116
2.6



Colon ca. CaCo-2
0.6



Colon ca. tissue(ODO3866)
6.6



Colon ca. HCC-2998
4.5



Gastric ca.* (liver met) NCI-N87
100.0



Bladder
6.7



Trachea
10.9



Kidney
0.8



Kidney (fetal)
5.5



Renal ca. 786-0
10.0



Renal ca. A498
17.8



Renal ca. RXF 393
11.0



Renal ca. ACHN
6.9



Renal ca. UO-31
14.4



Renal ca. TK-10
0.2



Liver
2.2



Liver (fetal)
5.8



Liver ca. (hepatoblast) HepG2
0.1



Lung
27.9



Lung (fetal)
14.0



Lung ca. (small cell) LX-1
3.4



Lung ca. (small cell) NCI-H69
0.2



Lung ca. (s. cell var.) SHP-77
3.0



Lung ca. (large cell)NCI-H460
9.7



Lung ca. (non-sm. cell) A549
0.8



Lung ca. (non-s. cell) NCI-H23
1.3



Lung ca. (non-s. cell) HOP-62
19.3



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
33.2



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
5.6



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
62.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
11.1



Breast ca. MDA-N
5.9



Ovary
3.5



Ovarian ca. OVCAR-3
1.7



Ovarian ca. OVCAR-4
0.1



Ovarian ca. OVCAR-5
5.9



Ovarian ca. OVCAR-8
3.6



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.6



Uterus
2.7



Placenta
59.0



Prostate
1.7



Prostate ca.* (bone met)PC-3
3.1



Testis
3.7



Melanoma Hs688(A).T
15.1



Melanoma* (met) Hs688(B).T
9.7



Melanoma UACC-62
1.4



Melanoma M14
0.1



Melanoma LOX IMVI
17.8



Melanoma* (met) SK-MEL-5
7.4



Adipose
5.1









Column A - Rel. Exp. (%) Ag1544, Run 146169090














TABLE AMC










Panel 2D











Tissue Name
A
B















Normal Colon
15.7
2.5



CC Well to Mod Diff (ODO3866)
5.1
0.8



CC Margin (ODO3866)
3.8
0.5



CC Gr.2 rectosigmoid (ODO3868)
3.3
1.2



CC Margin (ODO3868)
0.0
0.1



CC Mod Diff (ODO3920)
2.6
1.8



CC Margin (ODO3920)
3.0
2.2



CC Gr.2 ascend colon (ODO3921)
6.7
4.0



CC Margin (ODO3921)
1.6
0.9



CC from Partial Hepatectomy
6.0
5.4



(ODO4309) Mets



Liver Margin (ODO4309)
9.8
4.7



Colon mets to lung (OD04451-01)
7.3
7.2



Lung Margin (OD04451-02)
13.6
2.4



Normal Prostate 6546-1
3.5
2.2



Prostate Cancer (OD04410)
5.1
3.7



Prostate Margin (OD04410)
4.5
8.0



Prostate Cancer (OD04720-01)
6.3
7.1



Prostate Margin (OD04720-02)
8.1
11.5



Normal Lung 061010
34.2
49.0



Lung Met to Muscle (ODO4286)
25.7
58.2



Muscle Margin (ODO4286)
5.7
4.9



Lung Malignant Cancer (OD03126)
13.9
25.7



Lung Margin (OD03126)
39.0
32.3



Lung Cancer (OD04404)
27.4
15.1



Lung Margin (OD04404)
25.0
4.9



Lung Cancer (OD04565)
6.2
5.8



Lung Margin (OD04565)
12.9
17.1



Lung Cancer (OD04237-01)
27.5
17.6



Lung Margin (OD04237-02)
100.0
28.9



Ocular Mel Met to Liver (ODO4310)
3.3
3.9



Liver Margin (ODO4310)
5.0
5.6



Melanoma Mets to Lung (OD04321)
5.5
3.7



Lung Margin (OD04321)
35.8
32.1



Normal Kidney
12.1
4.8



Kidney Ca, Nuclear grade 2 (OD04338)
12.7
11.9



Kidney Margin (OD04338)
6.7
2.6



Kidney Ca Nuclear grade 1/2 (OD04339)
14.7
9.2



Kidney Margin (OD04339)
6.6
3.2



Kidney Ca, Clear cell type (OD04340)
7.3
9.4



Kidney Margin (OD04340)
8.2
5.1



Kidney Ca, Nuclear grade 3 (OD04348)
6.7
8.1



Kidney Margin (OD04348)
17.9
18.9



Kidney Cancer (OD04622-01)
7.5
15.9



Kidney Margin (OD04622-03)
1.9
0.7



Kidney Cancer (OD04450-01)
1.7
2.3



Kidney Margin (OD04450-03)
6.5
5.9



Kidney Cancer 8120607
4.5
2.5



Kidney Margin 8120608
0.7
0.8



Kidney Cancer 8120613
1.2
1.7



Kidney Margin 8120614
0.0
0.3



Kidney Cancer 9010320
4.7
1.2



Kidney Margin 9010321
2.1
0.7



Normal Uterus
2.7
1.6



Uterus Cancer 064011
6.0
6.3



Normal Thyroid
7.3
4.5



Thyroid Cancer 064010
36.3
29.9



Thyroid Cancer A302152
11.6
13.8



Thyroid Margin A302153
15.7
9.9



Normal Breast
5.2
3.9



Breast Cancer (OD04566)
5.0
3.3



Breast Cancer (OD04590-01)
8.6
2.3



Breast Cancer Mets (OD04590-03)
6.7
7.2



Breast Cancer Metastasis (OD04655-05)
6.6
5.6



Breast Cancer 064006
15.3
11.2



Breast Cancer 1024
1.9
0.9



Breast Cancer 9100266
3.1
3.5



Breast Margin 9100265
2.4
1.9



Breast Cancer A209073
5.1
7.2



Breast Margin A209073
5.4
1.2



Normal Liver
6.0
4.3



Liver Cancer 064003
4.7
1.1



Liver Cancer 1025
1.8
1.5



Liver Cancer 1026
0.0
0.5



Liver Cancer 6004-T
4.3
1.1



Liver Tissue 6004-N
4.2
1.3



Liver Cancer 6005-T
0.5
0.4



Liver Tissue 6005-N
1.6
1.0



Normal Bladder
11.3
12.7



Bladder Cancer 1023
1.8
1.3



Bladder Cancer A302173
50.0
16.7



Bladder Cancer (OD04718-01)
96.6
100.0



Bladder Normal Adjacent (OD04718-03)
11.1
4.6



Normal Ovary
0.3
0.4



Ovarian Cancer 064008
7.4
3.4



Ovarian Cancer (OD04768-07)
23.0
0.4



Ovary Margin (OD04768-08)
5.5
2.0



Normal Stomach
5.2
2.1



Gastric Cancer 9060358
1.8
0.4



Stomach Margin 9060359
2.3
1.3



Gastric Cancer 9060395
5.9
2.0



Stomach Margin 9060394
5.0
1.9



Gastric Cancer 9060397
6.0
2.6



Stomach Margin 9060396
2.1
1.5



Gastric Cancer 064005
10.9
10.4









Column A - Rel. Exp. (%) Ag1544, Run 145030196






Column B - Rel. Exp. (%) Ag1544, Run 145361330














TABLE AMD










Panel 4.1D










Tissue Name
A














Secondary Th1 act
7.9



Secondary Th2 act
11.7



Secondary Tr1 act
7.3



Secondary Th1 rest
0.8



Secondary Th2 rest
1.2



Secondary Tr1 rest
1.1



Primary Th1 act
6.7



Primary Th2 act
4.5



Primary Tr1 act
5.9



Primary Th1 rest
0.4



Primary Th2 rest
0.3



Primary Tr1 rest
0.3



CD45RA CD4 lymphocyte act
5.8



CD45RO CD4 lymphocyte act
6.5



CD8 lymphocyte act
2.5



Secondary CD8 lymphocyte rest
5.9



Secondary CD8 lymphocyte act
0.7



CD4 lymphocyte none
0.4



2ry Th1/Th2/Tr1_anti-CD95 CH11
1.7



LAK cells rest
23.8



LAK cells IL-2
2.0



LAK cells IL-2 + IL-12
3.1



LAK cells IL-2 + IFN gamma
5.6



LAK cells IL-2 + IL-18
6.6



LAK cells PMA/ionomycin
77.9



NK Cells IL-2 rest
1.5



Two Way MLR 3 day
21.6



Two Way MLR 5 day
18.9



Two Way MLR 7 day
5.1



PBMC rest
0.3



PBMC PWM
11.5



PBMC PHA-L
8.4



Ramos (B cell) none
0.2



Ramos (B cell) ionomycin
0.3



B lymphocytes PWM
2.8



B lymphocytes CD40L and IL-4
1.2



EOL-1 dbcAMP
0.1



EOL-1 dbcAMP PMA/ionomycin
0.2



Dendritic cells none
19.9



Dendritic cells LPS
58.2



Dendritic cells anti-CD40
12.6



Monocytes rest
0.1



Monocytes LPS
100.0



Macrophages rest
4.6



Macrophages LPS
32.3



HUVEC none
2.0



HUVEC starved
3.2



HUVEC IL-1beta
4.0



HUVEC IFN gamma
18.4



HUVEC TNF alpha + IFN gamma
19.5



HUVEC TNF alpha + IL4
2.8



HUVEC IL-11
2.0



Lung Microvascular EC none
7.5



Lung Microvascular EC TNFalpha + IL-1beta
3.5



Microvascular Dermal EC none
5.2



Microsvasular Dermal EC TNFalpha + IL-1beta
1.4



Bronchial epithelium TNFalpha + IL1beta
1.1



Small airway epithelium none
0.7



Small airway epithelium TNFalpha + IL-1beta
2.3



Coronery artery SMC rest
1.4



Coronery artery SMC TNFalpha + IL-1beta
1.8



Astrocytes rest
3.3



Astrocytes TNFalpha + IL-1beta
5.8



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.3



CCD1106 (Keratinocytes) none
5.4



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
19.9



Liver cirrhosis
0.2



NCI-H292 none
0.8



NCI-H292 IL-4
1.4



NCI-H292 IL-9
1.0



NCI-H292 IL-13
1.6



NCI-H292 IFN gamma
4.2



HPAEC none
2.4



HPAEC TNF alpha + IL-1 beta
4.7



Lung fibroblast none
0.8



Lung fibroblast TNF alpha + IL-1 beta
1.6



Lung fibroblast IL-4
1.5



Lung fibroblast IL-9
1.7



Lung fibroblast IL-13
1.1



Lung fibroblast IFN gamma
15.0



Dermal fibroblast CCD1070 rest
2.8



Dermal fibroblast CCD1070 TNF alpha
4.9



Dermal fibroblast CCD1070 IL-1 beta
4.0



Dermal fibroblast IFN gamma
3.7



Dermal fibroblast IL-4
0.7



Dermal Fibroblasts rest
0.2



Neutrophils TNFa + LPS
0.4



Neutrophils rest
0.6



Colon
0.2



Lung
0.6



Thymus
4.5



Kidney
0.5









Column A - Rel. Exp. (%) Ag1544, Run 209988812







Panel 1.3D Summary: Ag1544 The expression of this gene was highest in a sample derived from a gastric cancer cell line (NCI-H87) (CT=27.3). There was substantial expression found in lung cancer cell lines, a breast cancer cell line and placental tissue. The expression of this gene is useful as a marker to distinguish NCI-H87 cells from other samples in the panel. Therapeutic modulation of this gene or its protein product, through the use of small molecule drugs, antibodies or protein therapeutics is beneficial in the treatment of lung cancer or breast cancer.


Panel 2D Summary: Ag1544 The expression of this gene was highest expression associated with bladder cancer tissue and lung tissue derived samples. The expression of this gene is useful as a marker to distinguish between these samples and the rest of the samples in the panel. Therapeutic modulation of this gene, through the use of small molecule drugs, antibodies or protein therapeutics are beneficial in the treatment of bladder or lung cancer.


Panel 4.1D Summary: Ag1544 This transcript was expressed in LAK cells, and induced in LAK cells activated with PMA/ionomycin, dendritic cells treated with LPS, monocytes treated with LPS, Gamma interferon treated HUEC cells and keratinocytes treated with TNFalpha and IL-1beta. This transcript encodes a smaller isoform of B7-H1, an antigen presentation co-receptor. B7-H1 binds to PD-1 ligand on T cells, resulting in T cell activation and production of IL-10. Antibody or other types of therapeutics designed with B7-H1 are useful for blocking T cell activation and for the treatment of T cell-mediated diseases such as asthma, psoriasis, IBD and arthritis. Alternatively, agonistic therapeutics designed with this protein are useful for adjuvant or immuno-modulatory treatments.


AN. CG56110-07: B7-H1


Expression of full-length physical clone CG56110-07 was assessed using the primer-probe set Ag5282, described in Table ANA. Results of the RTQ-PCR runs are shown in Tables ANB and ANC.

TABLE ANAProbe Name Ag5282SEQStartIDPrimersSequencesLengthPositionNoForward5′-tcaagtcctgagtggagattagat-3′244911474ProbeTET-5′-tggtcatcccagaactacctctggca-3′-265401475TAMRAReverse5′-cccagaattaccaagtgagtcct-3′235811476









TABLE ANB










General_screening_panel_v1.5










Tissue Name
A














Adipose
0.0



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
5.1



Melanoma* M14
0.6



Melanoma* LOXIMVI
23.0



Melanoma* SK-MEL-5
3.6



Squamous cell carcinoma SCC-4
10.9



Testis Pool
0.0



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
1.2



Placenta
2.8



Uterus Pool
2.8



Ovarian ca. OVCAR-3
2.7



Ovarian ca. SK-OV-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
6.1



Ovarian ca. IGROV-1
5.4



Ovarian ca. OVCAR-8
1.3



Ovary
0.0



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
31.2



Breast ca. BT 549
3.6



Breast ca. T47D
0.0



Breast ca. MDA-N
3.7



Breast Pool
0.5



Trachea
1.1



Lung
1.2



Fetal Lung
1.3



Lung ca. NCI-N417
0.0



Lung ca. LX-1
2.7



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.0



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
0.8



Lung ca. NCI-H460
1.4



Lung ca. HOP-62
13.1



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
0.0



Liver ca. HepG2
0.0



Kidney Pool
3.1



Fetal Kidney
0.0



Renal ca. 786-0
5.1



Renal ca. A498
2.1



Renal ca. ACHN
1.3



Renal ca. UO-31
6.8



Renal ca. TK-10
0.7



Bladder
4.0



Gastric ca. (liver met.) NCI-N87
100.0



Gastric ca. KATO III
4.2



Colon ca. SW-948
0.0



Colon ca. SW480
2.2



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
3.6



Colon ca. CaCo-2
0.0



Colon cancer tissue
1.8



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
4.8



Small Intestine Pool
4.3



Stomach Pool
0.0



Bone Marrow Pool
0.9



Fetal Heart
3.9



Heart Pool
2.3



Lymph Node Pool
0.0



Fetal Skeletal Muscle
1.2



Skeletal Muscle Pool
3.1



Spleen Pool
3.9



Thymus Pool
3.9



CNS cancer (glio/astro) U87-MG
21.9



CNS cancer (glio/astro) U-118-MG
3.5



CNS cancer (neuro; met) SK-N-AS
1.4



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
2.5



CNS cancer (glio) SNB-19
8.7



CNS cancer (glio) SF-295
3.6



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
1.3



Brain (fetal)
0.0



Brain (Hippocampus) Pool
0.0



Cerebral Cortex Pool
2.1



Brain (Substantia nigra) Pool
0.0



Brain (Thalamus) Pool
0.9



Brain (whole)
0.0



Spinal Cord Pool
1.4



Adrenal Gland
2.2



Pituitary gland Pool
0.0



Salivary Gland
0.0



Thyroid (female)
2.5



Pancreatic ca. CAPAN2
12.3



Pancreas Pool
1.8









Column A - Rel. Exp. (%) Ag5282, Run 230565189














TABLE ANC










Panel 4.1D










Tissue Name
A














Secondary Th1 act
5.6



Secondary Th2 act
18.6



Secondary Tr1 act
4.9



Secondary Th1 rest
0.0



Secondary Th2 rest
0.3



Secondary Tr1 rest
0.9



Primary Th1 act
4.6



Primary Th2 act
4.1



Primary Tr1 act
9.7



Primary Th1 rest
0.0



Primary Th2 rest
0.8



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
9.8



CD45RO CD4 lymphocyte act
13.1



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
6.7



Secondary CD8 lymphocyte act
0.8



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
14.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
1.9



LAK cells IL-2 + IFN gamma
1.7



LAK cells IL-2 + IL-18
3.0



LAK cells PMA/ionomycin
76.3



NK Cells IL-2 rest
1.0



Two Way MLR 3 day
9.4



Two Way MLR 5 day
4.0



Two Way MLR 7 day
0.7



PBMC rest
0.0



PBMC PWM
6.1



PBMC PHA-L
6.1



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
3.6



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
10.2



Dendritic cells LPS
36.1



Dendritic cells anti-CD40
4.8



Monocytes rest
0.0



Monocytes LPS
100.0



Macrophages rest
2.6



Macrophages LPS
5.0



HUVEC none
2.2



HUVEC starved
3.8



HUVEC IL-1beta
1.7



HUVEC IFN gamma
25.5



HUVEC TNF alpha + IFN gamma
13.9



HUVEC TNF alpha + IL4
2.0



HUVEC IL-11
0.0



Lung Microvascular EC none
9.9



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.8



Microsvasular Dermal EC TNFalpha + IL-1beta
0.3



Bronchial epithelium TNFalpha + IL1beta
0.3



Small airway epithelium none
2.3



Small airway epithelium TNFalpha + IL-1beta
2.2



Coronery artery SMC rest
1.0



Coronery artery SMC TNFalpha + IL-1beta
1.9



Astrocytes rest
1.8



Astrocytes TNFalpha + IL-1beta
5.3



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
1.0



CCD1106 (Keratinocytes) none
5.5



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
18.8



Liver cirrhosis
0.0



NCI-H292 none
0.0



NCI-H292 IL-4
1.0



NCI-H292 IL-9
1.0



NCI-H292 IL-13
1.5



NCI-H292 IFN gamma
8.8



HPAEC none
0.9



HPAEC TNF alpha + IL-1 beta
6.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
1.0



Lung fibroblast IL-4
2.3



Lung fibroblast IL-9
1.6



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
17.0



Dermal fibroblast CCD1070 rest
6.6



Dermal fibroblast CCD1070 TNF alpha
9.4



Dermal fibroblast CCD1070 IL-1 beta
5.3



Dermal fibroblast IFN gamma
2.8



Dermal fibroblast IL-4
0.0



Dermal Fibroblasts rest
0.0



Neutrophils TNFa + LPS
0.9



Neutrophils rest
0.0



Colon
0.0



Lung
0.0



Thymus
0.0



Kidney
0.0









Column A - Rel. Exp. (%) Ag5282, Run 230510202







General_screening_panel_v1.5 Summary: Ag5282 The expression of this gene was highest in a sample derived from a gastric cancer cell line (NCI-H87) (CT=31).


There was relatively low expression in the remaining samples of panel 1.5. The expression of this gene is useful as a marker to distinguish NCI-H87 cells from other samples in the panel.


In addition, low expression of this gene was also seen in number of cancer cell lines derived from melanoma, pancreatic, gastric, renal, squamous cell carcinoma and lung cancers. Therapeutic modulation of this gene or its protein product is useful in the treatment of these cancers.


Panel 4.1D Summary: Ag5282 This transcript was not expressed in the normal tissue samples on this panel. The transcript was expressed in LAK cells, and induced in LAK cells activated with PMA/ionomycin, dendritic cells treated with LPS, monocytes treated with LPS, Gamma interferon treated HUVEC cells and keratinocytes treated with TNFalpha and IL-1beta. This transcript encodes a smaller isoform of B7-H1, an antigen presentation co-receptor. B7-H1 binds to PD-1 ligand on T cells and resulting in T cell activation and production of IL-10. Antibody or other types of therapeutics designed with B7-H1 are useful for blocking T cell activation and in the treatment of T cell-mediated diseases such as asthma, psoriasis, IBD and arthritis. Alternatively, agonistic therapeutics designed with this protein are useful for adjuvant like properties.


AO. CG56449-09: MEGF6/NOTCH like Isoform 7


Expression of gene CG56449-09 was assessed using the primer-probe sets Ag1153, Ag1937, Ag422 and Ag252 and, described in Tables AOA, AOB, AOC, and AOD. Results of the RTQ-PCR runs are shown in Tables AOE, AOF, and AOG.

TABLE AOAProbe Name Ag1513SEQStartIDPrimersSequencesLengthPositionNoForward5′-acacacgctcacagagaagttt-3′2215211477ProbeTET-5′-ctggatgactcctttggccatgact-3′-2515491478TAMRAReverse5′-ctgcagtcatcacaggtcaag-3′2115781479









TABLE AOB










Probe Name Ag1937
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ctgcagtcatcacaggtcaag-3′
21
1578
1480






Probe
TET-5′-ccaaaggagtcatccaggcagacaaa-3′-
26
1540
1481



TAMRA





Reverse
5′-gaacacacgctcacagagaag-3′
21
1519 1482
















TABLE AOC










Probe Name Ag422
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-tgaacaccccaggctcctac-3′
20
545
1483






Probe
TET-5′-cggcttccggctccacactgac-3′-
22
582
1484



TAMRA





Reverse
5′-taatggccaggcaggtcct-3′
19
607
1485
















TABLE AOD










Probe Name Ag252
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gagctgccgcaactcttcc-3′
19
1453
1486






Probe
TET-5′-cgcaactctgcctcttcctcatcgg-3′-
25
1490
1487



TAMRA





Reverse
5′-gacaaacttctctgtgagcgtgtg-3′
24
1522
1488
















TABLE AOE










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
13.9



Pancreas
4.7



Pancreatic ca. CAPAN 2
4.1



Adrenal gland
6.7



Thyroid
5.1



Salivary gland
13.6



Pituitary gland
5.5



Brain (fetal)
28.5



Brain (whole)
43.8



Brain (amygdala)
32.3



Brain (cerebellum)
42.9



Brain (hippocampus)
44.1



Brain (substantia nigra)
4.1



Brain (thalamus)
14.8



Cerebral Cortex
25.5



Spinal cord
2.6



glio/astro U87-MG
2.0



glio/astro U-118-MG
10.2



astrocytoma SW1783
10.6



neuro*; met SK-N-AS
1.7



astrocytoma SF-539
4.9



astrocytoma SNB-75
10.1



glioma SNB-19
6.6



glioma U251
8.4



glioma SF-295
1.4



Heart (fetal)
11.8



Heart
7.6



Skeletal muscle (fetal)
7.4



Skeletal muscle
0.0



Bone marrow
1.8



Thymus
12.1



Spleen
0.0



Lymph node
15.7



Colorectal
0.0



Stomach
4.9



Small intestine
13.7



Colon ca. SW480
12.0



Colon ca.* SW620(SW480 met)
2.9



Colon ca. HT29
2.3



Colon ca. HCT-116
2.9



Colon ca. CaCo-2
2.9



Colon ca. tissue(ODO3866)
2.9



Colon ca. HCC-2998
2.8



Gastric ca.* (liver met) NCI-N87
12.9



Bladder
4.1



Trachea
34.2



Kidney
4.7



Kidney (fetal)
22.8



Renal ca. 786-0
0.0



Renal ca. A498
19.5



Renal ca. RXF 393
53.2



Renal ca. ACHN
2.4



Renal ca. UO-31
1.4



Renal ca. TK-10
1.4



Liver
3.2



Liver (fetal)
1.4



Liver ca. (hepatoblast) HepG2
100.0



Lung
6.3



Lung (fetal)
10.2



Lung ca. (small cell) LX-1
2.8



Lung ca. (small cell) NCI-H69
64.2



Lung ca. (s. cell var.) SHP-77
5.9



Lung ca. (large cell)NCI-H460
6.4



Lung ca. (non-sm. cell) A549
3.3



Lung ca. (non-s. cell) NCI-H23
21.6



Lung ca. (non-s. cell) HOP-62
11.9



Lung ca. (non-s. cl) NCI-H522
40.9



Lung ca. (squam.) SW 900
1.7



Lung ca. (squam.) NCI-H596
23.3



Mammary gland
6.9



Breast ca.* (pl. ef) MCF-7
6.8



Breast ca.* (pl. ef) MDA-MB-231
47.0



Breast ca.* (pl. ef) T47D
0.0



Breast ca. BT-549
22.4



Breast ca. MDA-N
3.1



Ovary
0.6



Ovarian ca. OVCAR-3
7.1



Ovarian ca. OVCAR-4
9.5



Ovarian ca. OVCAR-5
2.2



Ovarian ca. OVCAR-8
20.6



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
9.7



Uterus
6.1



Placenta
4.9



Prostate
21.2



Prostate ca.* (bone met)PC-3
15.7



Testis
5.0



Melanoma Hs688(A).T
1.6



Melanoma* (met) Hs688(B).T
4.9



Melanoma UACC-62
2.7



Melanoma M14
3.2



Melanoma LOX IMVI
3.0



Melanoma* (met) SK-MEL-5
1.3



Adipose
0.0









Column A - Rel. Exp. (%) Ag252, Run 165628866














TABLE AOF










Panel 2D










Tissue Name
A














Normal Colon
11.3



CC Well to Mod Diff (ODO3866)
11.0



CC Margin (ODO3866)
1.6



CC Gr.2 rectosigmoid (ODO3868)
9.7



CC Margin (ODO3868)
2.5



CC Mod Diff (ODO3920)
20.9



CC Margin (ODO3920)
3.0



CC Gr.2 ascend colon (ODO3921)
3.2



CC Margin (ODO3921)
1.8



CC from Partial Hepatectomy (ODO4309) Mets
18.7



Liver Margin (ODO4309)
2.3



Colon mets to lung (OD04451-01)
14.0



Lung Margin (OD04451-02)
17.1



Normal Prostate 6546-1
20.6



Prostate Cancer (OD04410)
30.1



Prostate Margin (OD04410)
18.4



Prostate Cancer (OD04720-01)
36.9



Prostate Margin (OD04720-02)
24.0



Normal Lung 061010
15.3



Lung Met to Muscle (ODO4286)
1.5



Muscle Margin (ODO4286)
11.1



Lung Malignant Cancer (OD03126)
23.5



Lung Margin (OD03126)
19.5



Lung Cancer (OD04404)
10.5



Lung Margin (OD04404)
53.2



Lung Cancer (OD04565)
12.9



Lung Margin (OD04565)
23.8



Lung Cancer (OD04237-01)
4.9



Lung Margin (OD04237-02)
32.5



Ocular Mel Met to Liver (ODO4310)
2.0



Liver Margin (ODO4310)
5.4



Melanoma Mets to Lung (OD04321)
0.7



Lung Margin (OD04321)
24.5



Normal Kidney
8.3



Kidney Ca, Nuclear grade 2 (OD04338)
22.5



Kidney Margin (OD04338)
4.1



Kidney Ca Nuclear grade 1/2 (OD04339)
10.9



Kidney Margin (OD04339)
6.5



Kidney Ca, Clear cell type (OD04340)
26.8



Kidney Margin (OD04340)
10.4



Kidney Ca, Nuclear grade 3 (OD04348)
3.7



Kidney Margin (OD04348)
6.0



Kidney Cancer (OD04622-01)
100.0



Kidney Margin (OD04622-03)
6.5



Kidney Cancer (OD04450-01)
4.5



Kidney Margin (OD04450-03)
7.4



Kidney Cancer 8120607
3.5



Kidney Margin 8120608
8.7



Kidney Cancer 8120613
1.2



Kidney Margin 8120614
6.1



Kidney Cancer 9010320
12.5



Kidney Margin 9010321
9.9



Normal Uterus
18.9



Uterus Cancer 064011
15.6



Normal Thyroid
3.3



Thyroid Cancer 064010
6.9



Thyroid Cancer A302152
10.9



Thyroid Margin A302153
5.7



Normal Breast
42.3



Breast Cancer (OD04566)
26.6



Breast Cancer (OD04590-01)
21.8



Breast Cancer Mets (OD04590-03)
36.3



Breast Cancer Metastasis (OD04655-05)
14.9



Breast Cancer 064006
21.5



Breast Cancer 1024
42.0



Breast Cancer 9100266
11.0



Breast Margin 9100265
10.8



Breast Cancer A209073
14.9



Breast Margin A209073
13.0



Normal Liver
6.4



Liver Cancer 064003
0.0



Liver Cancer 1025
1.1



Liver Cancer 1026
19.6



Liver Cancer 6004-T
10.0



Liver Tissue 6004-N
6.0



Liver Cancer 6005-T
15.3



Liver Tissue 6005-N
3.3



Normal Bladder
19.2



Bladder Cancer 1023
5.6



Bladder Cancer A302173
3.4



Bladder Cancer (OD04718-01)
7.6



Bladder Normal Adjacent (OD04718-03)
9.3



Normal Ovary
2.4



Ovarian Cancer 064008
11.8



Ovarian Cancer (OD04768-07)
2.9



Ovary Margin (OD04768-08)
31.4



Normal Stomach
5.6



Gastric Cancer 9060358
0.0



Stomach Margin 9060359
1.9



Gastric Cancer 9060395
5.9



Stomach Margin 9060394
2.4



Gastric Cancer 9060397
18.6



Stomach Margin 9060396
2.8



Gastric Cancer 064005
1.5









Column A - Rel. Exp. (%) Ag252, Run 144791435














TABLE AOG










Panel 4D










Tissue Name
A
B
C













Secondary Th1 act
0.7
0.0
0.0


Secondary Th2 act
0.8
0.0
0.0


Secondary Tr1 act
0.5
0.0
4.0


Secondary Th1 rest
1.7
0.0
5.6


Secondary Th2 rest
14.0
10.0
15.2


Secondary Tr1 rest
6.2
0.0
5.7


Primary Th1 act
1.9
2.1
1.1


Primary Th2 act
2.1
2.3
9.9


Primary Tr1 act
0.2
0.6
4.5


Primary Th1 rest
16.3
10.0
15.7


Primary Th2 rest
7.4
9.1
21.9


Primary Tr1 rest
11.6
11.0
13.9


CD45RA CD4 lymphocyte act
13.7
9.4
16.6


CD45RO CD4 lymphocyte act
1.1
0.6
3.1


CD8 lymphocyte act
1.5
0.9
1.1


Secondary CD8 lymphocyte rest
4.0
3.3
5.0


Secondary CD8 lymphocyte act
0.0
0.0
0.0


CD4 lymphocyte none
19.8
18.6
30.8


2ry Th1/Th2/Tr1_anti-CD95 CH11
6.2
4.1
19.9


LAK cells rest
8.4
8.7
17.4


LAK cells IL-2
1.6
6.2
5.8


LAK cells IL-2 + IL-12
4.3
4.0
8.4


LAK cells IL-2 + IFN gamma
1.0
8.1
4.1


LAK cells IL-2 + IL-18
1.3
8.3
1.3


LAK cells PMA/ionomycin
2.6
1.2
2.5


NK Cells IL-2 rest
1.2
4.8
6.8


Two Way MLR 3 day
8.1
13.5
3.7


Two Way MLR 5 day
1.3
5.0
1.4


Two Way MLR 7 day
1.0
2.0
2.6


PBMC rest
3.0
3.7
7.8


PBMC PWM
5.2
2.3
3.6


PBMC PHA-L
1.0
1.9
2.6


Ramos (B cell) none
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0


B lymphocytes PWM
1.1
1.4
1.4


B lymphocytes CD40L and IL-4
2.1
3.7
8.1


EOL-1 dbcAMP
4.7
3.4
4.3


EOL-1 dbcAMP PMA/ionomycin
3.6
1.3
10.7


Dendritic cells none
1.7
1.9
3.3


Dendritic cells LPS
0.0
0.0
1.2


Dendritic cells anti-CD40
1.6
0.6
1.8


Monocytes rest
5.0
4.8
6.3


Monocytes LPS
0.4
0.6
1.4


Macrophages rest
12.3
9.0
13.8


Macrophages LPS
0.5
0.6
0.0


HUVEC none
4.2
22.5
15.6


HUVEC starved
17.3
22.7
17.1


HUVEC IL-1beta
2.9
2.0
3.5


HUVEC IFN gamma
27.2
18.4
25.9


HUVEC TNF alpha + IFN gamma
8.5
9.9
2.0


HUVEC TNF alpha + IL4
7.9
5.9
13.7


HUVEC IL-11
15.6
15.9
17.8


Lung Microvascular EC none
27.7
25.7
23.8


Lung Microvascular EC TNFalpha +
24.0
22.5
19.1


IL-1beta


Microvascular Dermal EC none
14.2
15.9
17.4


Microsvasular Dermal EC TNFalpha +
8.2
9.4
25.3


IL-1beta


Bronchial epithelium TNFalpha +
4.0
2.2
1.3


IL1beta


Small airway epithelium none
1.9
1.6
1.5


Small airway epithelium TNFalpha +
0.5
6.4
2.6


IL-1beta


Coronery artery SMC rest
23.2
29.7
33.9


Coronery artery SMC TNFalpha +
33.4
19.5
13.5


IL-1beta


Astrocytes rest
0.0
26.6
17.2


Astrocytes TNFalpha + IL-1beta
100.0
100.0
100.0


KU-812 (Basophil) rest
0.6
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.5
0.0


CCD1106 (Keratinocytes) none
1.9
0.6
3.5


CCD1106 (Keratinocytes) TNFalpha +
0.5
2.1
3.8


IL-1beta


Liver cirrhosis
9.1
12.2
13.0


Lupus kidney
3.0
2.6
3.8


NCI-H292 none
30.1
41.8
25.7


NCI-H292 IL-4
16.8
35.1
16.5


NCI-H292 IL-9
21.9
28.5
32.8


NCI-H292 IL-13
37.6
28.1
33.7


NCI-H292 IFN gamma
20.3
23.5
22.5


HPAEC none
36.1
23.5
31.6


HPAEC TNF alpha + IL-1 beta
22.7
11.1
24.5


Lung fibroblast none
10.7
15.0
14.4


Lung fibroblast TNF alpha + IL-1 beta
1.9
2.6
1.2


Lung fibroblast IL-4
11.0
9.7
7.7


Lung fibroblast IL-9
11.3
9.3
13.2


Lung fibroblast IL-13
7.3
4.5
17.4


Lung fibroblast IFN gamma
7.1
10.6
9.4


Dermal fibroblast CCD1070 rest
33.2
51.4
45.4


Dermal fibroblast CCD1070 TNF
24.0
31.9
21.3


alpha


Dermal fibroblast CCD1070 IL-1
34.6
34.4
46.3


beta


Dermal fibroblast IFN gamma
30.6
27.0
32.3


Dermal fibroblast IL-4
34.4
29.7
33.9


IBD Colitis 2
1.7
1.4
3.0


IBD Crohn's
0.7
0.0
1.3


Colon
12.9
9.2
25.0


Lung
25.7
55.5
42.9


Thymus
12.2
18.0
18.3


Kidney
26.8
39.2
25.3







Column A - Rel. Exp. (%) Ag1513, Run 163478079





Column B - Rel. Exp. (%) Ag1937, Run 161702009





Column C - Rel. Exp. (%) Ag422, Run 138056654







Panel 1.3D Summary: Ag252 Highest levels of expression of this gene were seen in a liver cell line HepG2(CT=30.27). Based on expression in this panel, this gene is involved in brain, colon, renal, lung, ovarian and prostate cancer as well as melanomas. Thus, expression of this is useful as a diagnostic marker for the presence of these cancers. Therapeutic inhibition using antibodies, protein therapeutics or small molecule drugs are of use in the treatment of these cancers.


This gene product also showed low but significant levels of expression in pancreas, adrenal, thyroid, pituitary, adult and fetal heart, and adult and fetal liver. This gene or its protein product is important for the pathogenesis, diagnosis, and/or treatment of metabolic and endocrine diseases, including obesity and Types 1 and 2 diabetes. Furthermore, this gene was more highly expressed in fetal (CT=34) skeletal muscle when compared to expression in the adult (CT=40) and is useful for the differentiation of the fetal and adult sources of this tissue.


In addition, this gene was expressed at moderate levels in the CNS. This gene encodes a mouse epidermal growth factor homolog, and increases axonal or dendritic outgrowth and synaptogenesis. This gene is of use in the treatment of clinical conditions associated with neuron loss such as head or spinal cord trauma, stroke, or any neurodegenerative disease.


Panel 2D Summary: Ag252 This gene was expressed at low levels in all the samples on this panel, with highest expression in a kidney cancer sample (CT=31.1). Gastric, liver and colon cancers expressed this gene at a higher level than the normal adjacent tissue from these organs. There was increased expression in normal lung and ovarian tissue when compared to the adjacent tumor samples. These data show that the expression of this gene is associated with gastric, liver and colon cancer and thus, therapeutic modulation of this gene product is of use in the treatment of these cancers. Conversely, absence of expression is associated with ovarian and lung cancer and is of use as a diagnostic marker for the presence of these cancers. Therapeutic modulation of this gene is of use in the treatment of these cancers.


Panel 4D Summary: This transcript was expressed at low levels in T cells, fibroblasts, endothelium, smooth muscle cells and T cells regardless of treatment. The transcript was also expressed in normal colon, lung and thymus. However, TNFalpha and IL-1beta induce the expression of the transcript in astrocytes. The trancritpt encodes a Notch like protein which functions in astrocyte differentiation and activation. (Colombatti Glia 2001 September; 35(3):224-33). Therapeutic regulation of this transcript or the design of therapeutics with the encoded protein is important in the treatment of multiple scelrosis or other inflammtory diseases of the CNS.


AP. CG5659401: Claudin-19 Like


Expression of gene CG56594-01 was assessed using the primer-probe set Ag2957, described in Table APA. Results of the RTQ-PCR runs are shown in Tables APB, APC and APD.

TABLE APAProbe Name Ag2957SEQStartIDPrimersSequencesLengthPositionNoForward5′-caggcctctgcactttgac-3′194381489ProbeTET-5′-ctgtctcgtggtatgccaccctggt-3′-254591490TAMRAReverse5′-ccaaattctgggttgaagaact-3′224921491









TABLE APB










General_screening_panel_v1.4










Tissue Name
A














Adipose
1.8



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
0.0



Melanoma* LOXIMVI
0.0



Melanoma* SK-MEL-5
0.0



Squamous cell carcinoma SCC-4
0.0



Testis Pool
0.0



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.0



Placenta
41.5



Uterus Pool
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. SK-OV-3
2.7



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca. OVCAR-8
0.0



Ovary
0.0



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
0.0



Breast ca. BT 549
1.7



Breast ca. T47D
0.0



Breast ca. MDA-N
0.0



Breast Pool
0.0



Trachea
2.8



Lung
2.9



Fetal Lung
24.5



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.9



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.0



Lung ca. NCI-H526
6.0



Lung ca. NCI-H23
0.0



Lung ca. NCI-H460
0.0



Lung ca. HOP-62
0.0



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
31.4



Liver ca. HepG2
7.6



Kidney Pool
1.7



Fetal Kidney
100.0



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
6.0



Bladder
0.0



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
4.6



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
24.8



Colon cancer tissue
1.4



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.0



Small Intestine Pool
0.0



Stomach Pool
0.0



Bone Marrow Pool
0.0



Fetal Heart
0.0



Heart Pool
0.0



Lymph Node Pool
0.0



Fetal Skeletal Muscle
8.0



Skeletal Muscle Pool
0.0



Spleen Pool
0.0



Thymus Pool
3.0



CNS cancer (glio/astro) U87-MG
0.0



CNS cancer (glio/astro) U-118-MG
0.0



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
0.0



CNS cancer (astro) SNB-75
0.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.0



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
0.0



Brain (fetal)
1.7



Brain (Hippocampus) Pool
4.7



Cerebral Cortex Pool
0.0



Brain (Substantia nigra) Pool
0.0



Brain (Thalamus) Pool
0.0



Brain (whole)
0.0



Spinal Cord Pool
22.4



Adrenal Gland
0.0



Pituitary gland Pool
0.0



Salivary Gland
0.0



Thyroid (female)
0.0



Pancreatic ca. CAPAN2
2.0



Pancreas Pool
0.0









Column A - Rel. Exp. (%) Ag2957, Run 216861284














TABLE APC










Panel 2D










Tissue Name
A














Normal Colon
0.0



CC Well to Mod Diff (ODO3866)
1.0



CC Margin (ODO3866)
0.0



CC Gr.2 rectosigmoid (ODO3868)
13.6



CC Margin (ODO3868)
1.9



CC Mod Diff (ODO3920)
0.0



CC Margin (ODO3920)
0.0



CC Gr.2 ascend colon (ODO3921)
0.0



CC Margin (ODO3921)
1.2



CC from Partial Hepatectomy (ODO4309)
0.0



Mets



Liver Margin (ODO4309)
0.0



Colon mets to lung (OD04451-01)
0.0



Lung Margin (OD04451-02)
0.0



Normal Prostate 6546-1
1.3



Prostate Cancer (OD04410)
0.0



Prostate Margin (OD04410)
2.6



Prostate Cancer (OD04720-01)
0.0



Prostate Margin (OD04720-02)
0.0



Normal Lung 061010
0.0



Lung Met to Muscle (ODO4286)
0.0



Muscle Margin (ODO4286)
0.0



Lung Malignant Cancer (OD03126)
0.0



Lung Margin (OD03126)
0.0



Lung Cancer (OD04404)
0.0



Lung Margin (OD04404)
0.0



Lung Cancer (OD04565)
0.0



Lung Margin (OD04565)
0.0



Lung Cancer (OD04237-01)
0.0



Lung Margin (OD04237-02)
0.0



Ocular Mel Met to Liver (ODO4310)
0.0



Liver Margin (ODO4310)
1.4



Melanoma Mets to Lung (OD04321)
0.0



Lung Margin (OD04321)
0.0



Normal Kidney
85.3



Kidney Ca, Nuclear grade 2 (OD04338)
6.2



Kidney Margin (OD04338)
32.3



Kidney Ca Nuclear grade 1/2 (OD04339)
13.5



Kidney Margin (OD04339)
92.0



Kidney Ca, Clear cell type (OD04340)
9.7



Kidney Margin (OD04340)
87.7



Kidney Ca, Nuclear grade 3 (OD04348)
0.0



Kidney Margin (OD04348)
100.0



Kidney Cancer (OD04622-01)
0.0



Kidney Margin (OD04622-03)
50.3



Kidney Cancer (OD04450-01)
0.0



Kidney Margin (OD04450-03)
58.6



Kidney Cancer 8120607
0.0



Kidney Margin 8120608
68.8



Kidney Cancer 8120613
0.0



Kidney Margin 8120614
24.3



Kidney Cancer 9010320
3.4



Kidney Margin 9010321
85.9



Normal Uterus
0.0



Uterus Cancer 064011
1.6



Normal Thyroid
0.0



Thyroid Cancer 064010
0.0



Thyroid Cancer A302152
0.0



Thyroid Margin A302153
0.0



Normal Breast
3.3



Breast Cancer (OD04566)
0.0



Breast Cancer (OD04590-01)
3.0



Breast Cancer Mets (OD04590-03)
0.0



Breast Cancer Metastasis (OD04655-05)
0.0



Breast Cancer 064006
0.0



Breast Cancer 1024
9.5



Breast Cancer 9100266
3.3



Breast Margin 9100265
5.4



Breast Cancer A209073
4.1



Breast Margin A209073
4.9



Normal Liver
0.0



Liver Cancer 064003
0.0



Liver Cancer 1025
0.0



Liver Cancer 1026
0.0



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
12.4



Liver Cancer 6005-T
5.7



Liver Tissue 6005-N
0.0



Normal Bladder
0.0



Bladder Cancer 1023
0.0



Bladder Cancer A302173
0.0



Bladder Cancer (OD04718-01)
0.0



Bladder Normal Adjacent (OD04718-03)
0.0



Normal Ovary
0.0



Ovarian Cancer 064008
0.0



Ovarian Cancer (OD04768-07)
28.7



Ovary Margin (OD04768-08)
0.0



Normal Stomach
0.0



Gastric Cancer 9060358
0.0



Stomach Margin 9060359
0.0



Gastric Cancer 9060395
0.0



Stomach Margin 9060394
0.0



Gastric Cancer 9060397
0.0



Stomach Margin 9060396
0.0



Gastric Cancer 064005
0.0









Column A - Rel. Exp. (%) Ag2957, Run 170858345














TABLE APD










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.8



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
1.7



Lupus kidney
5.8



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



IBD Colitis 2
0.0



IBD Crohn's
0.0



Colon
2.3



Lung
0.0



Thymus
100.0



Kidney
7.6









Column A - Rel. Exp. (%) Ag2957, Run 164306319







General_screening_panel_v1.4 Summary: Ag2957 Expression of this gene was restricted to placenta, fetal kidney and liver. Thus, expression of this gene is useful as a marker to differentiate between these samples and other samples on this panel. This gene shows no or very low expression in the cancer cell lines used in this panel and absence of expression of this gene or its protein product is useful as a diagnostic marker for cancer. This gene encodes a putative claudin. Claudins are components of tight junction strands. This specific pattern of expression indicates that this gene product is involved in the formation of TJ strands in these tissues.


Panel 2D Summary: Ag2957 This gene was consistently expressed in the normal kidney samples (CTs=32-33) but not in the adjacent kidney tumors on this panel. Thus, absence of expression of this is useful as a diagnostic marker for kidney cancer. Therapeutic modulation of the function or expression of this gene is useful as a treatment for this cancer.


Panel 4D Summary: Ag2957 The expression of this transcript was restricted to the thymus (CT=32.1) but not in T cells. Expression of this transcript is useful as a marker for this tissue.


AQ. CG5665308: Ficolin 1 Precursor


Expression of gene CG56653-08 was assessed using the primer-probe sets Ag1446, Ag4934 and Ag5886, described in Tables AQA, AQB and AQC. Results of the RTQ-PCR runs are shown in Tables AQD, AQE and AQF.

TABLE AQAProbe Name Ag1446SEQStartIDPrimersSequencesLengthPositionNoForward5′-cgctgtcctgctagtcttgtt-3′21371492ProbeTET-5′-atatcaagaacctgcctgcccaggct-3′-26631493TAMRAReverse5′-ccttcacctctggacatgtg-3′20941494









TABLE AQB










Probe Name Ag4934
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-cgctgtcctgctagtcttgtt-3′
21
37
1495






Probe
TET-5′-atatcaagaacctgcctgcccaggct-3′-
26
63
1496



TAMRA





Reverse
5′-ccttcacctctggacatgtg-3′
20
94
1497
















TABLE AQC










3Probe Name Ag5886
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-tggcccgttagagaattacc-3′
20
734
1498






Probe
TET-5′-acaaaggctcccagtaccagcttgta-3′-
26
695
1499



TAMRA





Reverse
5′-caaatcattcaaggtggctg-3′
20
661
1500
















TABLE AQD










AI_comprehensive panel_v1.0












Tissue Name
A
B
C
















110967 COPD-F
3.3
1.4
0.0



110980 COPD-F
4.8
1.0
1.8



110968 COPD-M
3.5
0.5
1.9



110977 COPD-M
14.9
6.3
5.4



110989 Emphysema-F
7.4
2.8
4.4



110992 Emphysema-F
3.6
2.2
0.2



110993 Emphysema-F
6.0
1.9
0.4



110994 Emphysema-F
3.3
1.6
0.2



110995 Emphysema-F
4.3
2.1
3.3



110996 Emphysema-F
1.6
1.2
0.7



110997 Asthma-M
7.6
2.6
0.6



111001 Asthma-F
15.6
8.3
9.0



111002 Asthma-F
20.3
6.7
6.4



111003 Atopic Asthma-F
10.7
3.7
2.6



111004 Atopic Asthma-F
7.2
0.0
1.7



111005 Atopic Asthma-F
3.7
1.0
1.8



111006 Atopic Asthma-F
0.0
0.0
0.3



111417 Allergy-M
4.1
1.7
1.5



112347 Allergy-M
0.4
0.0
0.2



112349 Normal Lung-F
0.5
0.0
0.1



112357 Normal Lung-F
6.7
2.8
2.2



112354 Normal Lung-M
0.5
0.4
0.6



112374 Crohns-F
2.7
0.0
0.4



112389 Match Control Crohns-F
3.5
1.7
2.4



112375 Crohns-F
0.0
0.2
1.3



112732 Match Control Crohns-F
10.2
2.8
3.2



112725 Crohns-M
11.0
2.4
2.3



112387 Match Control Crohns-M
4.9
2.3
2.7



112378 Crohns-M
0.0
0.3
0.2



112390 Match Control Crohns-M
0.8
0.3
0.6



112726 Crohns-M
5.1
2.2
2.1



112731 Match Control Crohns-M
3.1
3.3
2.1



112380 Ulcer Col-F
1.8
0.5
0.3



112734 Match Control Ulcer Col-F
40.6
19.1
9.7



112384 Ulcer Col-F
10.7
3.8
4.8



112737 Match Control Ulcer Col-F
2.0
1.5
2.0



112386 Ulcer Col-F
4.0
2.5
4.2



112738 Match Control Ulcer Col-F
54.3
12.0
7.7



112381 Ulcer Col-M
0.0
0.0
0.9



112735 Match Control Ulcer Col-M
3.4
1.8
4.3



112382 Ulcer Col-M
4.9
1.0
1.8



112394 Match Control Ulcer Col-M
3.7
1.4
1.9



112383 Ulcer Col-M
11.2
3.0
3.3



112736 Match Control Ulcer Col-M
4.6
0.5
0.7



112423 Psoriasis-F
23.3
11.7
12.9



112427 Match Control Psoriasis-F
11.0
2.1
4.1



112418 Psoriasis-M
2.9
1.1
2.6



112723 Match Control Psoriasis-M
0.9
0.4
0.2



112419 Psoriasis-M
5.5
2.6
3.9



112424 Match Control Psoriasis-M
1.8
0.9
1.5



112420 Psoriasis-M
10.2
3.3
9.2



112425 Match Control Psoriasis-M
5.6
1.8
2.6



104689 (MF) OA Bone-Backus
62.0
20.3
19.9



104690 (MF) Adj “Normal”
23.7
6.0
8.7



Bone-Backus



104691 (MF) OA Synovium-Backus
10.2
5.2
3.6



104692 (BA) OA Cartilage-Backus
0.8
0.0
0.3



104694 (BA) OA Bone-Backus
49.0
16.0
11.3



104695 (BA) Adj “Normal”
19.9
7.9
5.1



Bone-Backus



104696 (BA) OA Synovium-Backus
16.3
5.2
5.1



104700 (SS) OA Bone-Backus
100.0
100.0
100.0



104701 (SS) Adj “Normal”
29.7
7.0
8.7



Bone-Backus



104702 (SS) OA Synovium-Backus
27.7
9.3
11.0



117093 OA Cartilage Rep7
3.3
0.0
0.8



112672 OA Bone5
23.3
5.1
10.2



112673 OA Synovium5
7.1
2.6
2.9



112674 OA Synovial Fluid cells5
9.1
3.7
4.3



117100 OA Cartilage Rep14
2.4
1.3
1.5



112756 OA Bone9
3.9
0.7
0.7



112757 OA Synovium9
2.3
2.7
0.9



112758 OA Synovial Fluid Cells9
14.3
3.6
7.3



117125 RA Cartilage Rep2
3.6
1.0
1.6



113492 Bone2 RA
45.4
11.2
28.7



113493 Synovium2 RA
13.9
3.5
11.8



113494 Syn Fluid Cells RA
29.7
10.7
16.6



113499 Cartilage4 RA
14.9
6.8
10.7



113500 Bone4 RA
16.0
5.4
11.8



113501 Synovium4 RA
11.7
5.2
13.3



113502 Syn Fluid Cells4 RA
7.7
4.2
6.6



113495 Cartilage3 RA
26.6
12.5
16.5



113496 Bone3 RA
34.6
8.5
16.3



113497 Synovium3 RA
15.4
6.4
9.0



113498 Syn Fluid Cells3 RA
33.7
12.8
23.3



117106 Normal Cartilage Rep20
0.5
0.6
0.9



113663 Bone3 Normal
0.0
0.3
0.2



113664 Synovium3 Normal
0.0
0.0
0.0



113665 Syn Fluid Cells3 Normal
0.8
0.0
0.0



117107 Normal Cartilage Rep22
2.4
1.4
2.1



113667 Bone4 Normal
0.8
0.5
0.7



113668 Synovium4 Normal
0.6
0.9
0.2



113669 Syn Fluid Cells4 Normal
2.4
0.4
1.0









Column A - Rel. Exp. (%) Ag1446, Run 211195015






Column B - Rel. Exp. (%) Ag1446, Run 212650184






Column C - Rel. Exp. (%) Ag5886, Run 256261777














TABLE AQE










General_screening_panel_v1.5











Tissue Name
A
B















Adipose
48.6
48.6



Melanoma* Hs688(A).T
0.0
0.0



Melanoma* Hs688(B).T
0.0
0.0



Melanoma* M14
0.0
0.0



Melanoma* LOXIMVI
0.0
0.0



Melanoma* SK-MEL-5
4.8
0.0



Squamous cell carcinoma SCC-4
0.0
0.0



Testis Pool
1.4
1.3



Prostate ca.* (bone met) PC-3
0.0
0.0



Prostate Pool
9.3
5.6



Placenta
26.8
15.1



Uterus Pool
12.4
4.9



Ovarian ca. OVCAR-3
0.0
0.0



Ovarian ca. SK-OV-3
0.0
0.0



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
0.0
0.0



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca. OVCAR-8
0.0
0.0



Ovary
4.6
6.3



Breast ca. MCF-7
0.0
0.0



Breast ca. MDA-MB-231
0.0
0.0



Breast ca. BT 549
0.0
0.0



Breast ca. T47D
0.0
0.0



Breast ca. MDA-N
0.0
0.0



Breast Pool
25.3
15.9



Trachea
24.3
10.7



Lung
0.7
0.7



Fetal Lung
100.0
100.0



Lung ca. NCI-N417
0.0
0.0



Lung ca. LX-1
0.0
0.0



Lung ca. NCI-H146
0.0
0.0



Lung ca. SHP-77
0.0
0.0



Lung ca. A549
0.0
0.0



Lung ca. NCI-H526
0.0
0.0



Lung ca. NCI-H23
0.0
0.0



Lung ca. NCI-H460
0.0
0.0



Lung ca. HOP-62
0.0
0.0



Lung ca. NCI-H522
0.0
0.0



Liver
2.2
3.3



Fetal Liver
28.5
12.6



Liver ca. HepG2
0.0
0.0



Kidney Pool
18.6
7.9



Fetal Kidney
7.5
9.5



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.0
0.0



Bladder
6.4
4.0



Gastric ca. (liver met.) NCI-N87
0.0
0.0



Gastric ca. KATO III
0.0
0.0



Colon ca. SW-948
0.0
0.0



Colon ca. SW480
0.0
0.0



Colon ca.* (SW480 met) SW620
0.0
0.0



Colon ca. HT29
0.0
0.0



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
0.0
0.0



Colon cancer tissue
35.6
14.9



Colon ca. SW1116
0.0
0.0



Colon ca. Colo-205
0.0
0.0



Colon ca. SW-48
0.0
0.0



Colon Pool
68.8
34.4



Small Intestine Pool
9.4
3.8



Stomach Pool
7.5
4.8



Bone Marrow Pool
8.5
8.4



Fetal Heart
5.4
13.2



Heart Pool
9.9
3.4



Lymph Node Pool
8.9
9.5



Fetal Skeletal Muscle
7.2
12.6



Skeletal Muscle Pool
17.4
5.2



Spleen Pool
84.7
60.7



Thymus Pool
16.8
6.0



CNS cancer (glio/astro) U87-MG
0.0
0.0



CNS cancer (glio/astro) U-118-MG
0.0
0.0



CNS cancer (neuro; met) SK-N-AS
0.0
0.0



CNS cancer (astro) SF-539
0.0
0.0



CNS cancer (astro) SNB-75
0.0
0.0



CNS cancer (glio) SNB-19
0.0
0.0



CNS cancer (glio) SF-295
0.0
0.1



Brain (Amygdala) Pool
1.8
0.2



Brain (cerebellum)
1.4
1.1



Brain (fetal)
3.6
1.1



Brain (Hippocampus) Pool
2.0
0.8



Cerebral Cortex Pool
1.9
0.5



Brain (Substantia nigra) Pool
1.7
0.9



Brain (Thalamus) Pool
1.4
0.9



Brain (whole)
3.7
2.7



Spinal Cord Pool
3.1
0.6



Adrenal Gland
6.0
4.1



Pituitary gland Pool
0.7
0.7



Salivary Gland
5.9
2.7



Thyroid (female)
15.0
5.5



Pancreatic ca. CAPAN2
0.0
0.0



Pancreas Pool
27.9
9.7









Column A - Rel. Exp. (%) Ag4934, Run 228843453






Column B - Rel. Exp. (%) Ag5886, Run 247756990














TABLE AQF










Panel 4.1D









Tissue Name
A
B












Secondary Th1 act
0.0
0.0


Secondary Th2 act
0.0
0.0


Secondary Tr1 act
0.0
0.0


Secondary Th1 rest
0.0
0.0


Secondary Th2 rest
0.0
0.0


Secondary Tr1 rest
0.0
0.0


Primary Th1 act
0.0
0.0


Primary Th2 act
0.0
0.0


Primary Tr1 act
0.0
0.0


Primary Th1 rest
0.0
0.0


Primary Th2 rest
0.0
0.0


Primary Tr1 rest
0.0
0.0


CD45RA CD4 lymphocyte act
0.0
0.0


CD45RO CD4 lymphocyte act
0.0
0.0


CD8 lymphocyte act
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0


CD4 lymphocyte none
0.5
0.3


2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0


LAK cells rest
6.9
21.5


LAK cells IL-2
0.0
0.0


LAK cells IL-2 + IL-12
0.0
0.0


LAK cells IL-2 + IFN gamma
0.0
0.0


LAK cells IL-2 + IL-18
0.0
0.0


LAK cells PMA/ionomycin
8.2
66.0


NK Cells IL-2 rest
0.0
0.0


Two Way MLR 3 day
1.0
1.7


Two Way MLR 5 day
0.1
0.0


Two Way MLR 7 day
0.0
0.0


PBMC rest
19.2
24.0


PBMC PWM
0.0
0.0


PBMC PHA-L
0.0
0.0


Ramos (B cell) none
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0


B lymphocytes PWM
0.0
0.0


B lymphocytes CD40L and IL-4
0.0
0.1


EOL-1 dbcAMP
3.5
14.9


EOL-1 dbcAMP PMA/ionomycin
0.5
0.2


Dendritic cells none
1.0
4.5


Dendritic cells LPS
0.1
0.0


Dendritic cells anti-CD40
0.6
0.1


Monocytes rest
100.0
100.0


Monocytes LPS
4.8
15.6


Macrophages rest
4.1
5.0


Macrophages LPS
1.8
2.3


HUVEC none
0.0
0.0


HUVEC starved
0.0
0.0


HUVEC IL-1beta
0.0
0.0


HUVEC IFN gamma
0.0
0.0


HUVEC TNF alpha + IFN gamma
0.0
0.0


HUVEC TNF alpha + IL4
0.0
0.0


HUVEC IL-11
0.0
0.0


Lung Microvascular EC none
0.0
0.0


Lung Microvascular EC TNFalpha + IL-1beta
0.0
0.0


Microvascular Dermal EC none
0.0
0.0


Microsvasular Dermal EC TNFalpha + IL-1beta
0.0
0.0


Bronchial epithelium TNFalpha + IL1beta
0.0
0.0


Small airway epithelium none
0.0
0.0


Small airway epithelium TNFalpha + IL-1beta
0.0
0.0


Coronery artery SMC rest
0.0
0.0


Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0


Astrocytes rest
0.0
0.0


Astrocytes TNFalpha + IL-1beta
0.0
0.0


KU-812 (Basophil) rest
0.0
0.0


KU-812 (Basophil) PMA/ionomycin
0.0
0.0


CCD1106 (Keratinocytes) none
0.0
0.0


CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0
0.0


Liver cirrhosis
0.2
0.8


NCI-H292 none
0.0
0.0


NCI-H292 IL-4
0.0
0.0


NCI-H292 IL-9
0.0
0.0


NCI-H292 IL-13
0.0
0.0


NCI-H292 IFN gamma
0.0
0.0


HPAEC none
0.0
0.0


HPAEC TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast none
0.0
0.0


Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0


Lung fibroblast IL-4
0.0
0.0


Lung fibroblast IL-9
0.0
0.0


Lung fibroblast IL-13
0.0
0.0


Lung fibroblast IFN gamma
0.0
0.0


Dermal fibroblast CCD1070 rest
0.0
0.0


Dermal fibroblast CCD1070 TNF alpha
0.0
0.0


Dermal fibroblast CCD1070 IL-1 beta
0.1
0.0


Dermal fibroblast IFN gamma
0.1
0.0


Dermal fibroblast IL-4
0.3
0.0


Dermal Fibroblasts rest
0.1
0.0


Neutrophils TNFa + LPS
1.8
14.0


Neutrophils rest
3.8
47.3


Colon
0.0
0.0


Lung
0.3
0.2


Thymus
0.2
0.1


Kidney
0.1
0.2







Column A - Rel. Exp. (%) Ag4934, Run 223597255





Column B - Rel. Exp. (%) Ag5886, Run 256523048







AI_comprehensive panel_v1.0 Summary: Ag1446/Ag5886 Highest expression of this gene was seen in a bone sample from an OA patient (CTs=27-30.4). Moderate to high expression of this gene wass mainly seen in bone, synovium, synovial fluid and cartilage of OA and RA patients. Expression of this gene was low to undetectable in normal bone. Moderate to low expression was also found in colon, asthma, atopic asthma, emphysema, COPD and psoriasis samples. Ficolins are multimeric lectins that are capable of binding to bacteria and extracellular matrix proteins [Ohashi T. J Biol Chem 1997 May 30;272(22):14220-6]. Ficolin has been reported to function as a monocyte cell surface molecule that is important for binding to bacteria, elastin and for monocyte adhesion [Lu J, Immunobiology 1998 August;199(2):190-9]. This expression proves that ficolin is useful in alleviating inflammation in joints and other sites of inflammation. This gene functions as a protein therapeutic to reduce inflammation in osteoarthritis and rheumatoid arthritis. Ficolin also a role in the inflammation of joints in patients suffering from osteoarthritis (OA) and/or rheumatoid arthritis (RA). Antibodies against proteins encoded by this gene are useful for the prevention of tissue destruction mediated by ficolin activity during osteoarthritis and arthritis.


General_screening_panel_v1.5 Summary: Ag4934/Ag5886 Highest expression of this gene was detected in the fetal lung (CTs=27-31.5). Expression of this gene was also higher in fetal lung and fetal liver (CTs=27-33) than in their adult counterparts (CTs=32-38). Expression of this gene is useful as a marker to distinguish fetal from adult lung and liver. The relative overexpression of this gene in fetal tissue shows that the protein product enhances lung and liver growth or development in the fetus and also acts in a regenerative capacity in the adult. Therapeutic modulation of the protein encoded by this gene is useful in treatment of liver related diseases. Low but significant levels of expression was detected in tissues with metabolic/endocrine functions including adipose, heart, skeletal muscle, thyroid, pancreas and gastrointestinal tracts. Modulation of this gene or gene product is a treatment for metabolic or endocrine disease including obesity and Types 1 and 2 diabetes.


Low expression of this gene was also seen in some regions of central nervous system including whole and fetal brain, cerebellum, hipocampus, substantia nigra, thalamus and spinal cord. Therapeutic modulation of this gene or gene product is useful in the treatment of central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Panel 4.1D Summary: Ag4934/Ag5886 This gene was highly and selectively expressed in resting monocytes and to a lesser extent in macrophages and granulocytes (neutrophils and EOL cell line). This gene encodes a putative ficolin 1 precursor. Ficolin functions as a monocyte cell surface molecule that is important for binding to bacteria, elastin and monocyte adhesion. Protein therapeutics, antibodies, or small molecules designed with the protein encoded by this transcript are useful as opsinins to target and eliminate bacteria by complement-mediated destruction. These proteins are also important for the treatment of bacterial septicemia. In addition, ficolins may have the ability to bind to elastins. Elastins are functionally important for lung alveolar development and inactivation of these proteins can lead to emphysema-like disease. Therefore, antibodies, small molecules or protein therapeutics that target this gene or its protein product are useful for the prevention of tissue destruction mediated by ficolin activity during emphysema, asthma and arthritis.


AR. CG56806-01: Heparan Sulfate 6-Sulfotransferase 3 Like Gene


Expression of gene CG56806-01 was assessed using the primer-probe set Ag3024, described in Table ARA. Results of the RTQ-PCR runs are shown in Tables ARB, ARC, ARD, ARE and ARF.

TABLE ARAProbe Name Ag3024SEQStartIDPrimersSequencesLengthPositionNoForward5′-ggtgggctgctataacttgact-3′2210331501ProbeTET-5′-tgaaagaaacaccatcctgttgcaga-3′-2610691502TAMRAReverse5′-tgttcttcaggttgttctttgc-3′2210971503









TABLE ARB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
10.6



110980 COPD-F
9.5



110968 COPD-M
10.8



110977 COPD-M
16.7



110989 Emphysema-F
44.8



110992 Emphysema-F
3.8



110993 Emphysema-F
2.6



110994 Emphysema-F
1.0



110995 Emphysema-F
9.0



110996 Emphysema-F
1.3



110997 Asthma-M
1.0



111001 Asthma-F
33.4



111002 Asthma-F
37.9



111003 Atopic Asthma-F
41.5



111004 Atopic Asthma-F
21.2



111005 Atopic Asthma-F
39.0



111006 Atopic Asthma-F
8.5



111417 Allergy-M
26.6



112347 Allergy-M
8.6



112349 Normal Lung-F
9.2



112357 Normal Lung-F
0.6



112354 Normal Lung-M
1.3



112374 Crohns-F
4.0



112389 Match Control Crohns-F
1.3



112375 Crohns-F
4.5



112732 Match Control Crohns-F
0.0



112725 Crohns-M
9.9



112387 Match Control Crohns-M
15.3



112378 Crohns-M
9.7



112390 Match Control Crohns-M
23.7



112726 Crohns-M
2.9



112731 Match Control Crohns-M
1.4



112380 Ulcer Col-F
8.7



112734 Match Control Ulcer Col-F
0.7



112384 Ulcer Col-F
25.3



112737 Match Control Ulcer Col-F
3.0



112386 Ulcer Col-F
3.3



112738 Match Control Ulcer Col-F
0.6



112381 Ulcer Col-M
1.8



112735 Match Control Ulcer Col-M
32.8



112382 Ulcer Col-M
1.5



112394 Match Control Ulcer Col-M
1.8



112383 Ulcer Col-M
6.3



112736 Match Control Ulcer Col-M
1.1



112423 Psoriasis-F
12.7



112427 Match Control Psoriasis-F
37.1



112418 Psoriasis-M
6.1



112723 Match Control Psoriasis-M
0.0



112419 Psoriasis-M
16.6



112424 Match Control Psoriasis-M
17.8



112420 Psoriasis-M
100.0



112425 Match Control Psoriasis-M
26.2



104689 (MF) OA Bone-Backus
0.0



104690 (MF) Adj “Normal” Bone-Backus
0.4



104691 (MF) OA Synovium-Backus
3.0



104692 (BA) OA Cartilage-Backus
1.0



104694 (BA) OA Bone-Backus
0.0



104695 (BA) Adj “Normal” Bone-Backus
0.6



104696 (BA) OA Synovium-Backus
0.5



104700 (SS) OA Bone-Backus
0.2



104701 (SS) Adj “Normal” Bone-Backus
0.3



104702 (SS) OA Synovium-Backus
4.5



117093 OA Cartilage Rep7
42.0



112672 OA Bone5
18.4



112673 OA Synovium5
8.0



112674 OA Synovial Fluid cells5
2.4



117100 OA Cartilage Rep14
5.5



112756 OA Bone9
3.5



112757 OA Synovium9
2.1



112758 OA Synovial Fluid Cells9
11.9



117125 RA Cartilage Rep2
2.5



113492 Bone2 RA
1.7



113493 Synovium2 RA
0.0



113494 Syn Fluid Cells RA
1.6



113499 Cartilage4 RA
0.4



113500 Bone4 RA
1.3



113501 Synovium4 RA
0.0



113502 Syn Fluid Cells4 RA
0.2



113495 Cartilage3 RA
0.2



113496 Bone3 RA
0.6



113497 Synovium3 RA
0.9



113498 Syn Fluid Cells3 RA
0.6



117106 Normal Cartilage Rep20
14.1



113663 Bone3 Normal
8.5



113664 Synovium3 Normal
4.0



113665 Syn Fluid Cells3 Normal
5.7



117107 Normal Cartilage Rep22
13.8



113667 Bone4 Normal
17.2



113668 Synovium4 Normal
14.7



113669 Syn Fluid Cells4 Normal
7.6









Column A - Rel. Exp. (%) Ag3024, Run 248122026














TABLE ARC










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.4



Pancreas
0.0



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
2.6



Thyroid
0.7



Salivary gland
0.0



Pituitary gland
2.0



Brain (fetal)
11.0



Brain (whole)
54.7



Brain (amygdala)
27.2



Brain (cerebellum)
12.9



Brain (hippocampus)
36.3



Brain (substantia nigra)
5.7



Brain (thalamus)
64.2



Cerebral Cortex
100.0



Spinal cord
3.8



glio/astro U87-MG
0.0



glio/astro U-118-MG
14.7



astrocytoma SW1783
1.7



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
6.0



glioma SNB-19
0.6



glioma U251
0.0



glioma SF-295
0.0



Heart (fetal)
0.0



Heart
0.6



Skeletal muscle (fetal)
1.9



Skeletal muscle
0.4



Bone marrow
0.0



Thymus
0.0



Spleen
0.7



Lymph node
0.0



Colorectal
0.8



Stomach
6.9



Small intestine
1.4



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
1.0



Colon ca. HCC-2998
0.0



Gastric ca.* (liver met) NCI-N87
0.4



Bladder
0.7



Trachea
2.4



Kidney
0.6



Kidney (fetal)
0.2



Renal ca. 786-0
0.0



Renal ca. A498
2.4



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.7



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
0.0



Lung (fetal)
0.0



Lung ca. (small cell) LX-1
0.0



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.0



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.2



Lung ca. (non-s. cell) NCI-H23
0.0



Lung ca. (non-s. cell) HOP-62
0.6



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
2.1



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
0.4



Breast ca.* (pl. ef) MCF-7
11.0



Breast ca.* (pl. ef) MDA-MB-231
0.9



Breast ca.* (pl. ef) T47D
2.3



Breast ca. BT-549
1.0



Breast ca. MDA-N
0.0



Ovary
0.0



Ovarian ca. OVCAR-3
0.0



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.2



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0



Uterus
2.2



Placenta
0.0



Prostate
0.4



Prostate ca.* (bone met)PC-3
0.0



Testis
1.7



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.2



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
0.0









Column A - Rel. Exp. (%) Ag3024, Run 165517896














TABLE ARD










Panel 2D










Tissue Name
A














Normal Colon
4.7



CC Well to Mod Diff (ODO3866)
5.0



CC Margin (ODO3866)
3.1



CC Gr.2 rectosigmoid (ODO3868)
0.0



CC Margin (ODO3868)
0.3



CC Mod Diff (ODO3920)
0.0



CC Margin (ODO3920)
0.5



CC Gr.2 ascend colon (ODO3921)
3.1



CC Margin (ODO3921)
2.1



CC from Partial Hepatectomy (ODO4309) Mets
1.8



Liver Margin (ODO4309)
0.0



Colon mets to lung (OD04451-01)
0.0



Lung Margin (OD04451-02)
0.0



Normal Prostate 6546-1
3.7



Prostate Cancer (OD04410)
5.2



Prostate Margin (OD04410)
5.4



Prostate Cancer (OD04720-01)
11.9



Prostate Margin (OD04720-02)
6.4



Normal Lung 061010
0.0



Lung Met to Muscle (ODO4286)
0.6



Muscle Margin (ODO4286)
1.0



Lung Malignant Cancer (OD03126)
0.0



Lung Margin (OD03126)
1.6



Lung Cancer (OD04404)
0.0



Lung Margin (OD04404)
1.0



Lung Cancer (OD04565)
0.0



Lung Margin (OD04565)
0.0



Lung Cancer (OD04237-01)
8.4



Lung Margin (OD04237-02)
0.0



Ocular Mel Met to Liver (ODO4310)
2.5



Liver Margin (ODO4310)
0.0



Melanoma Mets to Lung (OD04321)
0.4



Lung Margin (OD04321)
0.5



Normal Kidney
23.7



Kidney Ca, Nuclear grade 2 (OD04338)
1.2



Kidney Margin (OD04338)
10.6



Kidney Ca Nuclear grade 1/2 (OD04339)
0.0



Kidney Margin (OD04339)
7.7



Kidney Ca, Clear cell type (OD04340)
9.3



Kidney Margin (OD04340)
17.2



Kidney Ca, Nuclear grade 3 (OD04348)
2.3



Kidney Margin (OD04348)
5.1



Kidney Cancer (OD04622-01)
0.0



Kidney Margin (OD04622-03)
2.8



Kidney Cancer (OD04450-01)
0.0



Kidney Margin (OD04450-03)
17.8



Kidney Cancer 8120607
1.7



Kidney Margin 8120608
1.3



Kidney Cancer 8120613
100.0



Kidney Margin 8120614
5.3



Kidney Cancer 9010320
0.8



Kidney Margin 9010321
6.3



Normal Uterus
0.7



Uterus Cancer 064011
9.4



Normal Thyroid
5.7



Thyroid Cancer 064010
0.3



Thyroid Cancer A302152
2.0



Thyroid Margin A302153
23.5



Normal Breast
7.9



Breast Cancer (OD04566)
7.4



Breast Cancer (OD04590-01)
0.0



Breast Cancer Mets (OD04590-03)
3.6



Breast Cancer Metastasis (OD04655-05)
3.8



Breast Cancer 064006
7.1



Breast Cancer 1024
6.2



Breast Cancer 9100266
27.7



Breast Margin 9100265
4.3



Breast Cancer A209073
30.8



Breast Margin A209073
3.0



Normal Liver
1.3



Liver Cancer 064003
0.4



Liver Cancer 1025
0.0



Liver Cancer 1026
0.0



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
1.1



Liver Cancer 6005-T
0.0



Liver Tissue 6005-N
0.0



Normal Bladder
2.8



Bladder Cancer 1023
0.0



Bladder Cancer A302173
2.2



Bladder Cancer (OD04718-01)
0.7



Bladder Normal Adjacent (OD04718-03)
0.4



Normal Ovary
0.0



Ovarian Cancer 064008
1.4



Ovarian Cancer (OD04768-07)
0.0



Ovary Margin (OD04768-08)
3.3



Normal Stomach
17.8



Gastric Cancer 9060358
1.0



Stomach Margin 9060359
13.6



Gastric Cancer 9060395
7.5



Stomach Margin 9060394
4.1



Gastric Cancer 9060397
13.4



Stomach Margin 9060396
6.7



Gastric Cancer 064005
1.2









Column A - Rel. Exp. (%) Ag3024, Run 163577593














TABLE ARE










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
1.6



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
0.6



SNB-78- Glioma
0.0



SF-268- Glioblastoma
0.4



T98G- Glioblastoma
14.1



SK-N-SH- Neuroblastoma (metastasis)
0.2



SF-295- Glioblastoma
0.4



Cerebellum
15.3



Cerebellum
5.4



NCI-H292- Mucoepidermoid lung carcinoma
0.0



DMS-114- Small cell lung cancer
0.0



DMS-79- Small cell lung cancer
3.3



NCI-H146- Small cell lung cancer
0.0



NCI-H526- Small cell lung cancer
57.4



NCI-N417- Small cell lung cancer
14.4



NCI-H82- Small cell lung cancer
0.0



NCI-H157- Squamous cell lung cancer
0.0



(metastasis)



NCI-H1155- Large cell lung cancer
2.9



NCI-H1299- Large cell lung cancer
0.3



NCI-H727- Lung carcinoid
15.9



NCI-UMC-11- Lung carcinoid
1.2



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
0.0



KM12- Colon cancer
0.0



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
100.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
1.1



NCI-SNU-5- Gastric carcinoma
0.0



KATO III- Gastric carcinoma
0.0



NCI-SNU-16- Gastric carcinoma
0.4



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.0



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
0.0



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
0.2



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma
0.0



(metastasis)



ES-2- Ovarian clear cell carcinoma
0.3



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
0.6



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.2



TF-1- Erythroleukemia
0.0



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
32.8



SW 839- Clear cell renal carcinoma
0.2



Rhabdoid kidney tumor
0.0



Hs766T- Pancreatic carcinoma (LN
0.0



metastasis)



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver
1.5



metastasis)



BxPC-3- Pancreatic adenocarcinoma
0.0



HPAC- Pancreatic adenocarcinoma
0.3



MIA PaCa-2- Pancreatic carcinoma
0.0



CFPAC-1- Pancreatic ductal adenocarcinoma
0.0



PANC-1- Pancreatic epithelioid ductal
0.8



carcinoma



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
0.0



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
0.0



A204- Rhabdomyosarcoma
0.0



HT-1080- Fibrosarcoma
0.3



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
4.6



SJRH30- Rhabdomyosarcoma (met to bone marrow)
0.2



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
0.0



DU 145- Prostate carcinoma (brain
0.0



metastasis)



MDA-MB-468- Breast adenocarcinoma
1.3



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp. (%) Ag3024, Run 164886426














TABLE ARF










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
4.4



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
4.7



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
5.3



HUVEC none
24.7



HUVEC starved
11.8



HUVEC IL-1beta
0.0



HUVEC IFN gamma
31.4



HUVEC TNF alpha + IFN gamma
14.9



HUVEC TNF alpha + IL4
8.8



HUVEC IL-11
11.4



Lung Microvascular EC none
10.1



Lung Microvascular EC TNFalpha + IL-1beta
8.7



Microvascular Dermal EC none
11.3



Microsvasular Dermal EC TNFalpha + IL-1beta
3.0



Bronchial epithelium TNFalpha + IL1beta
5.3



Small airway epithelium none
3.7



Small airway epithelium TNFalpha + IL-1beta
66.0



Coronery artery SMC rest
21.0



Coronery artery SMC TNFalpha + IL-1beta
10.4



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
17.7



Lupus kidney
5.6



NCI-H292 none
3.2



NCI-H292 IL-4
0.0



NCI-H292 IL-9
10.4



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
6.3



HPAEC TNF alpha + IL-1 beta
3.2



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
4.1



Dermal fibroblast IFN gamma
6.1



Dermal fibroblast IL-4
8.7



IBD Colitis 2
0.0



IBD Crohn's
7.7



Colon
24.1



Lung
14.5



Thymus
100.0



Kidney
0.0









Column A - Rel. Exp. (%) Ag3024, Run 162427416







AI_comprehensive panel_v1.0 Summary: Ag3024 This gene was found at low but significant levels in lung tissue from COPD, emphysema and asthma patients. This expression was consistent with panel 4D which showed expression in small airway epithelium. Therefore, this gene is useful as a marker or a target for lung inflammatory diseases.


Panel 1.3D Summary: Ag3024 Expression of this gene, a heparin sulfate proteoglycan homolog, was highly brain preferential and plays a role for this gene product in CNS processes. Heparin sulfate proteoglycans (HSPGs) are a component of amyloid plaques in Alzheimer's disease. The interaction of apoE with HSPGs has also been implicated in the pathogenesis of Alzheimer's disease and plays a role in neuronal repair. apoE has an HSPG-binding site highly complementary to heparan sulfates rich in N- and O-sulfo groups. (Libeu C P, E. J Biol Chem 2001 October 19;276(42):39138-44). Enzymes that influence the structure of HSPGs, such as the protein product of this gene, will influence protein agregation and the functional processes underlying Alzheimer's disease. Agents that target and modulate the activity of this gene or gene product are effective in the treatment of neurodegenerative diseases including Alzheimer's disease. This gene was also expressed in breast and brain cancer cell lines at low but significant levels. The expression of this gene is of use as a marker for breast and brain cancer. Therapeutic inhibition of the activity of the product of this gene, through the use of antibodies or small molecule drugs, is useful in the therapy of brain and breast cancer.


Panel 2D Summary: Ag3024 This gene was expressed at low but significant levels in most of the samples on this panel, with highest expression in a kidney cancer sample (CT=30.6). Significant levels of expression were also seen in samples derived from breast and gastric cancer samples. Expression of this gene is of use as a marker for breast and gastric cancer. Therapeutic inhibition of the activity of the product of this gene or gene product, through the use of antibodies or small molecule drugs, is of use in the therapy of breast and gastric cancer.


Panel 3D Summary: Ag3024 This gene was expressed at low but significant levels in cell lines from a renal carcinoma, colon cancer, glioblastoma and three lung cancer lines. Thus, this gene is useful as a marker as well as a target for inhibition of these cancers.


Panel 4D Summary: Ag3024 This gene, a heparin Sulfate 6-Sulfotransferase 3 homolog, was expressed at low but significant levels in thymus and small airway epithelium treated with TNFalpha+IL-1beta (CTs=34). The gene product is of use as a marker for thymus or activated small airway epithelium.


AS. CG56904-01: Secreted LRR (Leucine-rich Repeat) Protein


Expression of gene CG56904-01 was assessed using the primer-probe sets Ag3048, and Ag6717, described in Tables ASA and ASB. Results of the RTQ-PCR runs are shown in Tables ASC, ASD, ASE, ASF, and ASG.

TABLE ASAProbe Name Ag3048SEQStartIDPrimersSequencesLengthPositionNoForward5′-cacaaccagctgacagacagt-3′214901504ProbeTET-5′-ccaccaccttcagcaagctgcatag-3′-255211505Reverse5′-gggagagatccaggtattcaag-3′225471506









TABLE ASB










Probe Name Ag6717
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gctcacaacaagaacaatctc-3
21
406
1507






Probe
TET-5′-aaggtgccccgaggagc-3′-TAMRA
17
433
1508





Reverse
5′-cacggagttgagtctgg-3′
17
459
1509
















TABLE ASC










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
0.0



110980 COPD-F
0.7



110968 COPD-M
1.0



110977 COPD-M
0.8



110989 Emphysema-F
0.8



110992 Emphysema-F
6.3



110993 Emphysema-F
0.3



110994 Emphysema-F
0.0



110995 Emphysema-F
12.7



110996 Emphysema-F
6.6



110997 Asthma-M
2.6



111001 Asthma-F
0.4



111002 Asthma-F
1.9



111003 Atopic Asthma-F
1.6



111004 Atopic Asthma-F
5.2



111005 Atopic Asthma-F
2.8



111006 Atopic Asthma-F
0.3



111417 Allergy-M
0.4



112347 Allergy-M
0.0



112349 Normal Lung-F
0.0



112357 Normal Lung-F
8.5



112354 Normal Lung-M
0.9



112374 Crohns-F
4.0



112389 Match Control Crohns-F
17.3



112375 Crohns-F
4.2



112732 Match Control Crohns-F
1.1



112725 Crohns-M
0.0



112387 Match Control Crohns-M
3.6



112378 Crohns-M
0.0



112390 Match Control Crohns-M
0.5



112726 Crohns-M
0.0



112731 Match Control Crohns-M
1.4



112380 Ulcer Col-F
0.9



112734 Match Control Ulcer Col-F
4.9



112384 Ulcer Col-F
6.8



112737 Match Control Ulcer Col-F
0.7



112386 Ulcer Col-F
7.0



112738 Match Control Ulcer Col-F
5.1



112381 Ulcer Col-M
0.0



112735 Match Control Ulcer Col-M
0.0



112382 Ulcer Col-M
11.8



112394 Match Control Ulcer Col-M
1.6



112383 Ulcer Col-M
30.8



112736 Match Control Ulcer Col-M
6.3



112423 Psoriasis-F
0.7



112427 Match Control Psoriasis-F
1.4



112418 Psoriasis-M
0.6



112723 Match Control Psoriasis-M
4.8



112419 Psoriasis-M
0.9



112424 Match Control Psoriasis-M
0.9



112420 Psoriasis-M
6.4



112425 Match Control Psoriasis-M
0.4



104689 (MF) OA Bone-Backus
54.7



104690 (MF) Adj “Normal” Bone-Backus
23.3



104691 (MF) OA Synovium-Backus
5.3



104692 (BA) OA Cartilage-Backus
0.0



104694 (BA) OA Bone-Backus
100.0



104695 (BA) Adj “Normal” Bone-Backus
80.1



104696 (BA) OA Synovium-Backus
27.4



104700 (SS) OA Bone-Backus
21.6



104701 (SS) Adj “Normal” Bone-Backus
90.8



104702 (SS) OA Synovium-Backus
76.3



117093 OA Cartilage Rep7
7.9



112672 OA Bone5
0.7



112673 OA Synovium5
0.4



112674 OA Synovial Fluid cells5
0.0



117100 OA Cartilage Rep14
0.0



112756 OA Bone9
8.6



112757 OA Synovium9
0.7



112758 OA Synovial Fluid Cells9
3.8



117125 RA Cartilage Rep2
0.5



113492 Bone2 RA
3.7



113493 Synovium2 RA
1.2



113494 Syn Fluid Cells RA
1.9



113499 Cartilage4 RA
1.4



113500 Bone4 RA
3.1



113501 Synovium4 RA
2.4



113502 Syn Fluid Cells4 RA
1.2



113495 Cartilage3 RA
2.0



113496 Bone3 RA
2.0



113497 Synovium3 RA
1.7



113498 Syn Fluid Cells3 RA
2.5



117106 Normal Cartilage Rep20
0.4



113663 Bone3 Normal
0.0



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
0.0



117107 Normal Cartilage Rep22
0.0



113667 Bone4 Normal
0.4



113668 Synovium4 Normal
0.5



113669 Syn Fluid Cells4 Normal
1.0









Column A - Rel. Exp. (%) Ag6717, Run 283839606














TABLE ASD










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
2.0



Pancreas
0.0



Pancreatic ca. CAPAN 2
0.3



Adrenal gland
0.0



Thyroid
0.5



Salivary gland
0.6



Pituitary gland
0.5



Brain (fetal)
0.9



Brain (whole)
2.4



Brain (amygdala)
2.9



Brain (cerebellum)
7.0



Brain (hippocampus)
2.1



Brain (substantia nigra)
4.5



Brain (thalamus)
4.6



Cerebral Cortex
1.0



Spinal cord
7.6



glio/astro U87-MG
2.8



glio/astro U-118-MG
5.9



astrocytoma SW1783
2.7



neuro*; met SK-N-AS
1.1



astrocytoma SF-539
100.0



astrocytoma SNB-75
41.2



glioma SNB-19
0.4



glioma U251
6.7



glioma SF-295
7.3



Heart (fetal)
5.1



Heart
1.3



Skeletal muscle (fetal)
4.7



Skeletal muscle
2.6



Bone marrow
0.9



Thymus
0.9



Spleen
0.3



Lymph node
0.0



Colorectal
2.9



Stomach
0.8



Small intestine
1.0



Colon ca. SW480
0.8



Colon ca.* SW620(SW480 met)
1.9



Colon ca. HT29
0.3



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.2



Colon ca. tissue(ODO3866)
13.2



Colon ca. HCC-2998
4.1



Gastric ca.* (liver met) NCI-N87
1.5



Bladder
3.0



Trachea
3.7



Kidney
0.0



Kidney (fetal)
0.6



Renal ca. 786-0
3.8



Renal ca. A498
17.7



Renal ca. RXF 393
12.5



Renal ca. ACHN
0.1



Renal ca. UO-31
1.1



Renal ca. TK-10
0.5



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.2



Lung
1.4



Lung (fetal)
7.1



Lung ca. (small cell) LX-1
0.2



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.9



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
0.4



Lung ca. (non-s. cell) HOP-62
41.2



Lung ca. (non-s. cl) NCI-H522
1.6



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
15.9



Breast ca.* (pl. ef) MCF-7
3.5



Breast ca.* (pl. ef) MDA-MB-231
2.0



Breast ca.* (pl. ef) T47D
0.3



Breast ca. BT-549
0.0



Breast ca. MDA-N
0.3



Ovary
3.8



Ovarian ca. OVCAR-3
0.2



Ovarian ca. OVCAR-4
0.2



Ovarian ca. OVCAR-5
4.3



Ovarian ca. OVCAR-8
0.8



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
1.5



Uterus
0.6



Placenta
0.9



Prostate
3.0



Prostate ca.* (bone met)PC-3
1.7



Testis
3.9



Melanoma Hs688(A).T
32.8



Melanoma* (met) Hs688(B).T
31.4



Melanoma UACC-62
1.8



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
4.3









Column A - Rel. Exp. (%) Ag3048, Run 168017062














TABLE ASE










Panel 2D










Tissue Name
A














Normal Colon
4.7



CC Well to Mod Diff (ODO3866)
12.0



CC Margin (ODO3866)
1.5



CC Gr.2 rectosigmoid (ODO3868)
10.1



CC Margin (ODO3868)
0.8



CC Mod Diff (ODO3920)
8.4



CC Margin (ODO3920)
2.6



CC Gr.2 ascend colon (ODO3921)
13.6



CC Margin (ODO3921)
2.1



CC from Partial Hepatectomy (ODO4309) Mets
3.1



Liver Margin (ODO4309)
0.1



Colon mets to lung (OD04451-01)
3.3



Lung Margin (OD04451-02)
1.1



Normal Prostate 6546-1
4.1



Prostate Cancer (OD04410)
19.8



Prostate Margin (OD04410)
4.5



Prostate Cancer (OD04720-01)
9.7



Prostate Margin (OD04720-02)
7.5



Normal Lung 061010
3.3



Lung Met to Muscle (ODO4286)
24.5



Muscle Margin (ODO4286)
1.7



Lung Malignant Cancer (OD03126)
11.6



Lung Margin (OD03126)
3.1



Lung Cancer (OD04404)
22.2



Lung Margin (OD04404)
7.2



Lung Cancer (OD04565)
36.1



Lung Margin (OD04565)
2.0



Lung Cancer (OD04237-01)
12.6



Lung Margin (OD04237-02)
6.7



Ocular Mel Met to Liver (ODO4310)
0.0



Liver Margin (ODO4310)
0.4



Melanoma Mets to Lung (OD04321)
0.0



Lung Margin (OD04321)
1.7



Normal Kidney
0.8



Kidney Ca, Nuclear grade 2 (OD04338)
0.3



Kidney Margin (OD04338)
1.5



Kidney Ca Nuclear grade 1/2 (OD04339)
0.2



Kidney Margin (OD04339)
0.9



Kidney Ca, Clear cell type (OD04340)
0.4



Kidney Margin (OD04340)
1.0



Kidney Ca, Nuclear grade 3 (OD04348)
10.3



Kidney Margin (OD04348)
0.4



Kidney Cancer (OD04622-01)
34.4



Kidney Margin (OD04622-03)
0.2



Kidney Cancer (OD04450-01)
0.0



Kidney Margin (OD04450-03)
0.2



Kidney Cancer 8120607
9.0



Kidney Margin 8120608
0.5



Kidney Cancer 8120613
0.1



Kidney Margin 8120614
0.4



Kidney Cancer 9010320
13.1



Kidney Margin 9010321
0.9



Normal Uterus
0.5



Uterus Cancer 064011
1.0



Normal Thyroid
3.3



Thyroid Cancer 064010
0.7



Thyroid Cancer A302152
4.5



Thyroid Margin A302153
0.8



Normal Breast
20.0



Breast Cancer (OD04566)
6.5



Breast Cancer (OD04590-01)
10.4



Breast Cancer Mets (OD04590-03)
11.4



Breast Cancer Metastasis (OD04655-05)
1.0



Breast Cancer 064006
23.2



Breast Cancer 1024
25.7



Breast Cancer 9100266
16.4



Breast Margin 9100265
24.0



Breast Cancer A209073
32.3



Breast Margin A209073
33.2



Normal Liver
0.0



Liver Cancer 064003
0.0



Liver Cancer 1025
0.0



Liver Cancer 1026
4.2



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
2.9



Liver Cancer 6005-T
2.2



Liver Tissue 6005-N
0.0



Normal Bladder
23.8



Bladder Cancer 1023
11.8



Bladder Cancer A302173
7.2



Bladder Cancer (OD04718-01)
12.7



Bladder Normal Adjacent (OD04718-03)
4.9



Normal Ovary
3.5



Ovarian Cancer 064008
100.0



Ovarian Cancer (OD04768-07)
2.5



Ovary Margin (OD04768-08)
8.1



Normal Stomach
1.5



Gastric Cancer 9060358
0.5



Stomach Margin 9060359
0.8



Gastric Cancer 9060395
7.5



Stomach Margin 9060394
1.9



Gastric Cancer 9060397
27.0



Stomach Margin 9060396
1.5



Gastric Cancer 064005
3.3









Column A - Rel. Exp. (%) Ag3048, Run 170858352














TABLE ASF










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
6.5



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
18.0



XF-498- CNS
1.4



SNB-78- Glioma
100.0



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
24.1



SK-N-SH- Neuroblastoma (metastasis)
32.3



SF-295- Glioblastoma
2.0



Cerebellum
3.0



Cerebellum
4.2



NCI-H292- Mucoepidermoid lung carcinoma
38.4



DMS-114- Small cell lung cancer
1.7



DMS-79- Small cell lung cancer
3.0



NCI-H146- Small cell lung cancer
3.3



NCI-H526- Small cell lung cancer
0.0



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
0.0



NCI-H157- Squamous cell lung cancer
1.4



(metastasis)



NCI-H1155- Large cell lung cancer
1.8



NCI-H1299- Large cell lung cancer
2.8



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
0.0



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
7.3



KM12- Colon cancer
1.6



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.6



SW1116- Colon adenocarcinoma
0.8



LS 174T- Colon adenocarcinoma
2.5



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
2.7



NCI-SNU-5- Gastric carcinoma
1.6



KATO III- Gastric carcinoma
1.8



NCI-SNU-16- Gastric carcinoma
1.4



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.9



MKN-45- Gastric carcinoma
5.6



NCI-N87- Gastric carcinoma
2.3



OVCAR-5- Ovarian carcinoma
4.8



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma
0.0



(metastasis)



ES-2- Ovarian clear cell carcinoma
0.0



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
2.6



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
2.3



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.7



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
2.5



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
2.3



Hs766T- Pancreatic carcinoma (LN
1.5



metastasis)



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver
0.8



metastasis)



BxPC-3- Pancreatic adenocarcinoma
0.7



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.7



CFPAC-1- Pancreatic ductal adenocarcinoma
1.7



PANC-1- Pancreatic epithelioid ductal
6.7



carcinoma



T24- Bladder carcinma (transitional cell)
6.1



5637- Bladder carcinoma
2.1



HT-1197- Bladder carcinoma
1.7



UM-UC-3- Bladder carcinma (transitional cell)
0.7



A204- Rhabdomyosarcoma
5.4



HT-1080- Fibrosarcoma
11.7



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
7.5



SJRH30- Rhabdomyosarcoma (met to bone
0.0



marrow)



A431- Epidermoid carcinoma
5.1



WM266-4- Melanoma
16.6



DU 145- Prostate carcinoma (brain
0.0



metastasis)



MDA-MB-468- Breast adenocarcinoma
2.5



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.6



CAL 27- Squamous cell carcinoma of tongue
0.5









Column A - Rel. Exp. (%) Ag3048, Run 172133336














TABLE ASG








Panel 4D


















Monocytes LPS
0.0



Macrophages rest
1.9



Macrophages LPS
1.3



HUVEC none
0.0



HUVEC starved
2.9



Colon
2.0



Lung
36.1



Thymus
0.6



Kidney
1.7










AI_comprehensive panel_v1.0 Summary: Ag6717 Prominent expression was seen in bone and synovium samples from osteoarthritis patients, with highest expression in an OA bone sample (CT=31). Thus, targeting this gene or its protein product is useful in the treatment of osteoarthritis.


Panel 1.3D Summary: Ag3048 The expression of this gene was highest in a sample derived from a brain cancer cell line (SF-539) (CT=29.4). There was substantial expression associated with samples derived from another brain cancer cell line, two melanoma cell lines and a lung cancer cell line. The expression of this gene is useful as a marker to distinguish SF-539 cells from other samples in the panel. Therapeutic modulation of this gene, through the use of small molecule drugs, protein therapeuitcs or antibodies is of benefit in treatment of brain or lung cancer or melanoma.


This gene, a leucine-rich repeat homolog, was expressed at low levels in the CNS. The leucine-rich repeat region proteins have been implicated in axonal guidance. This gene has therapeutic utility in the treatment of any CNS disorder involving neuronal loss, specfically by guiding/enhancing compensatory synaptogenesis and fiber outgrowth, including such clinical conditions as Alzheimer's, Parkinson's, or Huntington's diseases, stroke, head and spinal cord trauma, vascular dementia or spinocerebellar ataxia.


Panel 2D Summary: Ag3048 The expression of this gene was highest in a sample derived from an ovarian cancer (CT=29). There was substantial expression associated with lung cancer, prostate cancer and colon cancer samples. Of note is the differential expression in the lung, colon and prostate cancer samples compared to their respective normal adjacent tissue. The expression of this gene is useful as a marker to distinguish this ovarian cancer sample from other samples in the panel. The expression of this gene or its protein product is useful as a marker to distinguish colon, prostate or lung cancer samples from their normal adjacent tissue. Therapeutic modulation of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies is benefical in the treatment of ovarian, lung, prostate or colon cancer.


Panel 3D Sumunary: Ag3048 The expression of this gene was highest in a sample derived from a brain cancer cell line (SNB-78) (CT=30.2). There was substantial expression associated with other brain cancer cell line samples and a lung cancer cell line sample. The expression of this gene is useful as a marker to distinguish SNB-78 cells from other samples in the panel. Therapeutic modulation of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies is useful in the treatment of brain or lung cancer.


Panel 4D Summary: Ag3048 This gene, a secreted leucine-rich repeat (LRR) protein, was expressed selectively at moderate levels (CT range 29-31) in several resting and cytokine-activated epithelial and connective tissue cells of lung and skin. This gene product is useful as a therapeutic protein as well as a target for therapeutic antibodies and small molecules, for the reduction or elimination of the symptoms in patients with chronic obstructive pulmonary disease, asthma, emphysema, or psoriasis.


AT. CG56904-04: LRR Protein


Expression of gene CG56904-04 was assessed using the primer-probe set Ag3048, described in Table ATA. Results of the RTQ-PCR runs are shown in Tables ATB, ATC, ATD and ATE.

TABLE ATAProbe Name Ag3048SEQStartIDPrimersSequencesLengthPositionNoForward5′-cacaaccagctgacagacagt-3′217241510ProbeTET-5′-ccaccaccttcagcaagctgcatag-3′-257551511TAMRAReverse5′-gggagagatccaggtattcaag-3′227811512









TABLE ATB










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
2.0



Pancreas
0.0



Pancreatic ca. CAPAN 2
0.3



Adrenal gland
0.0



Thyroid
0.5



Salivary gland
0.6



Pituitary gland
0.5



Brain (fetal)
0.9



Brain (whole)
2.4



Brain (amygdala)
2.9



Brain (cerebellum)
7.0



Brain (hippocampus)
2.1



Brain (substantia nigra)
4.5



Brain (thalamus)
4.6



Cerebral Cortex
1.0



Spinal cord
7.6



glio/astro U87-MG
2.8



glio/astro U-118-MG
5.9



astrocytoma SW1783
2.7



neuro*; met SK-N-AS
1.1



astrocytoma SF-539
100.0



astrocytoma SNB-75
41.2



glioma SNB-19
0.4



glioma U251
6.7



glioma SF-295
7.3



Heart (fetal)
5.1



Heart
1.3



Skeletal muscle (fetal)
4.7



Skeletal muscle
2.6



Bone marrow
0.9



Thymus
0.9



Spleen
0.3



Lymph node
0.0



Colorectal
2.9



Stomach
0.8



Small intestine
1.0



Colon ca. SW480
0.8



Colon ca.* SW620(SW480 met)
1.9



Colon ca. HT29
0.3



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.2



Colon ca. tissue(ODO3866)
13.2



Colon ca. HCC-2998
4.1



Gastric ca.* (liver met) NCI-N87
1.5



Bladder
3.0



Trachea
3.7



Kidney
0.0



Kidney (fetal)
0.6



Renal ca. 786-0
3.8



Renal ca. A498
17.7



Renal ca. RXF 393
12.5



Renal ca. ACHN
0.1



Renal ca. UO-31
1.1



Renal ca. TK-10
0.5



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.2



Lung
1.4



Lung (fetal)
7.1



Lung ca. (small cell) LX-1
0.2



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
0.9



Lung ca. (large cell)NCI-H460
0.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
0.4



Lung ca. (non-s. cell) HOP-62
41.2



Lung ca. (non-s. cl) NCI-H522
1.6



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
15.9



Breast ca.* (pl. ef) MCF-7
3.5



Breast ca.* (pl. ef) MDA-MB-231
2.0



Breast ca.* (pl. ef) T47D
0.3



Breast ca. BT-549
0.0



Breast ca. MDA-N
0.3



Ovary
3.8



Ovarian ca. OVCAR-3
0.2



Ovarian ca. OVCAR-4
0.2



Ovarian ca. OVCAR-5
4.3



Ovarian ca. OVCAR-8
0.8



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
1.5



Uterus
0.6



Placenta
0.9



Prostate
3.0



Prostate ca.* (bone met)PC-3
1.7



Testis
3.9



Melanoma Hs688(A).T
32.8



Melanoma* (met) Hs688(B).T
31.4



Melanoma UACC-62
1.8



Melanoma M14
0.0



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
0.0



Adipose
4.3









Column A - Rel. Exp. (%) Ag3048, Run 168017062














TABLE ATC










Panel 2D










Tissue Name
A














Normal Colon
4.7



CC Well to Mod Diff (ODO3866)
12.0



CC Margin (ODO3866)
1.5



CC Gr.2 rectosigmoid (ODO3868)
10.1



CC Margin (ODO3868)
0.8



CC Mod Diff (ODO3920)
8.4



CC Margin (ODO3920)
2.6



CC Gr.2 ascend colon (ODO3921)
13.6



CC Margin (ODO3921)
2.1



CC from Partial Hepatectomy (ODO4309) Mets
3.1



Liver Margin (ODO4309)
0.1



Colon mets to lung (OD04451-01)
3.3



Lung Margin (OD04451-02)
1.1



Normal Prostate 6546-1
4.1



Prostate Cancer (OD04410)
19.8



Prostate Margin (OD04410)
4.5



Prostate Cancer (OD04720-01)
9.7



Prostate Margin (OD04720-02)
7.5



Normal Lung 061010
3.3



Lung Met to Muscle (ODO4286)
24.5



Muscle Margin (ODO4286)
1.7



Lung Malignant Cancer (OD03126)
11.6



Lung Margin (OD03126)
3.1



Lung Cancer (OD04404)
22.2



Lung Margin (OD04404)
7.2



Lung Cancer (OD04565)
36.1



Lung Margin (OD04565)
2.0



Lung Cancer (OD04237-01)
12.6



Lung Margin (OD04237-02)
6.7



Ocular Mel Met to Liver (ODO4310)
0.0



Liver Margin (ODO4310)
0.4



Melanoma Mets to Lung (OD04321)
0.0



Lung Margin (OD04321)
1.7



Normal Kidney
0.8



Kidney Ca, Nuclear grade 2 (OD04338)
0.3



Kidney Margin (OD04338)
1.5



Kidney Ca Nuclear grade 1/2 (OD04339)
0.2



Kidney Margin (OD04339)
0.9



Kidney Ca, Clear cell type (OD04340)
0.4



Kidney Margin (OD04340)
1.0



Kidney Ca, Nuclear grade 3 (OD04348)
10.3



Kidney Margin (OD04348)
0.4



Kidney Cancer (OD04622-01)
34.4



Kidney Margin (OD04622-03)
0.2



Kidney Cancer (OD04450-01)
0.0



Kidney Margin (OD04450-03)
0.2



Kidney Cancer 8120607
9.0



Kidney Margin 8120608
0.5



Kidney Cancer 8120613
0.1



Kidney Margin 8120614
0.4



Kidney Cancer 9010320
13.1



Kidney Margin 9010321
0.9



Normal Uterus
0.5



Uterus Cancer 064011
1.0



Normal Thyroid
3.3



Thyroid Cancer 064010
0.7



Thyroid Cancer A302152
4.5



Thyroid Margin A302153
0.8



Normal Breast
20.0



Breast Cancer (OD04566)
6.5



Breast Cancer (OD04590-01)
10.4



Breast Cancer Mets (OD04590-03)
11.4



Breast Cancer Metastasis (OD04655-05)
1.0



Breast Cancer 064006
23.2



Breast Cancer 1024
25.7



Breast Cancer 9100266
16.4



Breast Margin 9100265
24.0



Breast Cancer A209073
32.3



Breast Margin A209073
33.2



Normal Liver
0.0



Liver Cancer 064003
0.0



Liver Cancer 1025
0.0



Liver Cancer 1026
4.2



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
2.9



Liver Cancer 6005-T
2.2



Liver Tissue 6005-N
0.0



Normal Bladder
23.8



Bladder Cancer 1023
11.8



Bladder Cancer A302173
7.2



Bladder Cancer (OD04718-01)
12.7



Bladder Normal Adjacent (OD04718-03)
4.9



Normal Ovary
3.5



Ovarian Cancer 064008
100.0



Ovarian Cancer (OD04768-07)
2.5



Ovary Margin (OD04768-08)
8.1



Normal Stomach
1.5



Gastric Cancer 9060358
0.5



Stomach Margin 9060359
0.8



Gastric Cancer 9060395
7.5



Stomach Margin 9060394
1.9



Gastric Cancer 9060397
27.0



Stomach Margin 9060396
1.5



Gastric Cancer 064005
3.3









Column A - Rel. Exp. (%) Ag3048, Run 170858352














TABLE ATD










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
6.5



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
18.0



XF-498- CNS
1.4



SNB-78- Glioma
100.0



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
24.1



SK-N-SH- Neuroblastoma (metastasis)
32.3



SF-295- Glioblastoma
2.0



Cerebellum
3.0



Cerebellum
4.2



NCI-H292- Mucoepidermoid lung
38.4



carcinoma



DMS-114- Small cell lung cancer
1.7



DMS-79- Small cell lung cancer
3.0



NCI-H146- Small cell lung cancer
3.3



NCI-H526- Small cell lung cancer
0.0



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
0.0



NCI-H157- Squamous cell lung cancer
1.4



(metastasis)



NCI-H1155- Large cell lung cancer
1.8



NCI-H1299- Large cell lung cancer
2.8



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
0.0



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
7.3



KM12- Colon cancer
1.6



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.6



SW1116- Colon adenocarcinoma
0.8



LS 174T- Colon adenocarcinoma
2.5



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
2.7



NCI-SNU-5- Gastric carcinoma
1.6



KATO III- Gastric carcinoma
1.8



NCI-SNU-16- Gastric carcinoma
1.4



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.9



MKN-45- Gastric carcinoma
5.6



NCI-N87- Gastric carcinoma
2.3



OVCAR-5- Ovarian carcinoma
4.8



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma
0.0



(metastasis)



ES-2- Ovarian clear cell carcinoma
0.0



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
2.6



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
2.3



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.7



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
2.5



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
2.3



Hs766T- Pancreatic carcinoma (LN
1.5



metastasis)



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver
0.8



metastasis)



BxPC-3- Pancreatic adenocarcinoma
0.7



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.7



CFPAC-1- Pancreatic ductal adenocarcinoma
1.7



PANC-1- Pancreatic epithelioid ductal
6.7



carcinoma



T24- Bladder carcinma (transitional cell)
6.1



5637- Bladder carcinoma
2.1



HT-1197- Bladder carcinoma
1.7



UM-UC-3- Bladder carcinma (transitional cell)
0.7



A204- Rhabdomyosarcoma
5.4



HT-1080- Fibrosarcoma
11.7



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
7.5



SJRH30- Rhabdomyosarcoma (met to bone
0.0



marrow)



A431- Epidermoid carcinoma
5.1



WM266-4- Melanoma
16.6



DU 145- Prostate carcinoma (brain
0.0



metastasis)



MDA-MB-468- Breast adenocarcinoma
2.5



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.6



CAL 27- Squamous cell carcinoma of tongue
0.5









Column A - Rel. Exp. (%) Ag3048, Run 172133336














TABLE ATE










Panel 4D










Tissue Name
A














Secondary Th1 act
0.4



Secondary Th2 act
0.0



Secondary Tr1 act
0.6



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.9



Primary Tr1 rest
0.6



CD45RA CD4 lymphocyte act
8.6



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.6



Secondary CD8 lymphocyte act
0.6



CD4 lymphocyte none
0.5



2ry Th1/Th2/Tr1_anti-CD95 CH11
1.3



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.8



PBMC rest
0.8



PBMC PWM
0.0



PBMC PHA-L
2.2



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.8



B lymphocytes PWM
1.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
1.8



Dendritic cells LPS
1.7



Dendritic cells anti-CD40
6.8



Monocytes rest
1.2



Monocytes LPS
0.0



Macrophages rest
1.9



Macrophages LPS
1.3



HUVEC none
0.0



HUVEC starved
2.9



HUVEC IL-1beta
0.0



HUVEC IFN gamma
1.9



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
1.2



HUVEC IL-11
0.3



Lung Microvascular EC none
3.9



Lung Microvascular EC TNFalpha + IL-1beta
1.2



Microvascular Dermal EC none
0.6



Microsvasular Dermal EC TNFalpha + IL-1beta
1.4



Bronchial epithelium TNFalpha + IL1beta
28.7



Small airway epithelium none
39.2



Small airway epithelium TNFalpha + IL-1beta
84.7



Coronery artery SMC rest
17.9



Coronery artery SMC TNFalpha + IL-1beta
5.5



Astrocytes rest
6.1



Astrocytes TNFalpha + IL-1beta
5.5



KU-812 (Basophil) rest
0.7



KU-812 (Basophil) PMA/ionomycin
3.8



CCD1106 (Keratinocytes) none
79.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
97.3



Liver cirrhosis
4.3



Lupus kidney
3.4



NCI-H292 none
87.1



NCI-H292 IL-4
77.4



NCI-H292 IL-9
82.9



NCI-H292 IL-13
54.0



NCI-H292 IFN gamma
49.3



HPAEC none
0.7



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
35.1



Lung fibroblast TNF alpha + IL-1 beta
7.0



Lung fibroblast IL-4
46.0



Lung fibroblast IL-9
38.7



Lung fibroblast IL-13
26.8



Lung fibroblast IFN gamma
68.8



Dermal fibroblast CCD1070 rest
100.0



Dermal fibroblast CCD1070 TNF alpha
59.9



Dermal fibroblast CCD1070 IL-1 beta
39.8



Dermal fibroblast IFN gamma
17.0



Dermal fibroblast IL-4
30.6



IBD Colitis 2
0.0



IBD Crohn's
0.7



Colon
2.0



Lung
36.1



Thymus
0.6



Kidney
1.7









Column A - Rel. Exp. (%) Ag3048, Run 164315038







Panel 1.3D Summary: Ag3048 The expression of this gene was highest in a sample derived from a brain cancer cell line (SF-539) (CT=29.4). There was substantial expression associated with samples derived from another brain cancer cell line, two melanoma cell lines and a lung cancer cell line. The expression of this gene is useful as a marker to distinguish SF-539 cells from other samples in the panel. Therapeutic modulation of this gene, through the use of small molecule drugs, protein therapeuitcs or antibodies is of benefit in treatment of brain or lung cancer or melanoma.


This gene, a leucine-rich repeat homolog, was expressed at low levels in the CNS. The leucine-rich repeat region proteins have been implicated in axonal guidance. This gene has therapeutic utility in the treatment of any CNS disorder involving neuronal loss, specfically by guiding/enhancing compensatory synaptogenesis and fiber outgrowth, including such clinical conditions as Alzheimer's, Parkinson's, or Huntington's diseases, stroke, head and spinal cord trauma, vascular dementia or spinocerebellar ataxia.


Panel 2D Summary: Ag3048 The expression of this gene was highest in a sample derived from an ovarian cancer (CT=29). There was substantial expression associated with lung cancer, prostate cancer and colon cancer samples. Of note is the differential expression in the lung, colon and prostate cancer samples compared to their respective normal adjacent tissue. The expression of this gene is useful as a marker to distinguish this ovarian cancer sample from other samples in the panel. The expression of this gene or its protein product is useful as a marker to distinguish colon, prostate or lung cancer samples from their normal adjacent tissue. Therapeutic modulation of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies is benefical in the treatment of ovarian, lung, prostate or colon cancer.


Panel 3D Summary: Ag3048 The expression of this gene was highest in a sample derived from a brain cancer cell line (SNB-78) (CT=30.2). There was substantial expression associated with other brain cancer cell line samples and a lung cancer cell line sample. The expression of this gene is useful as a marker to distinguish SNB-78 cells from other samples in the panel. Therapeutic modulation of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies is useftil in the treatment of brain or lung cancer.


Panel 4D Summary: Ag3048 This gene, a secreted leucine-rich repeat (LRR) protein, was expressed selectively at moderate levels (CT range 29-31) in several resting and cytokine-activated epithelial and connective tissue cells of lung and skin. This gene product is useful as a therapeutic protein as well as a target for therapeutic antibodies and small molecules, for the reduction or elimination of the symptoms in patients with chronic obstructive pulmonary disease, asthma, emphysema, or psoriasis.


AU. CG56914-01: Thrombospondin


Expression of gene CG56914-01 was assessed using the primer-probe sets Ag3108 and Ag3899, described in Tables AUA and AUB. Results of the RTQ-PCR runs are shown in Tables AUC, AUD, AUE, AUF, AUG.

TABLE AUAProbe Name Ag3108SEQStartIDPrimersSequencesLengthPositionNoForward5′-attccattgcccaaattaaca-3′217031513ProbeTET-5′-ccttcaataacaatattattccagccca-3′-287281514TAMRAReverse5′-actgtgtccattcacactgtca-3′227591515









TABLE AUB










Probe Name Ag3899
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ccattgcccaaattaacatg-3′
20
706
1516






Probe
TET-5′-ccttcaataacaatattattccagccca-3′-
28
728
1517



TAMRA





Reverse
5′-actgtgtccattcacactgtca-3′
22
759
1518
















TABLE AUC










General_screening_panel_v_1.4










Tissue Name
A














Adipose
1.0



Melanoma* Hs688(A).T
33.9



Melanoma* Hs688(B).T
8.4



Melanoma* M14
12.9



Melanoma* LOXIMVI
0.1



Melanoma* SK-MEL-5
58.6



Squamous cell carcinoma SCC-4
0.0



Testis Pool
0.6



Prostate ca.* (bone met) PC-3
0.2



Prostate Pool
0.4



Placenta
0.1



Uterus Pool
0.1



Ovarian ca. OVCAR-3
0.4



Ovarian ca. SK-OV-3
0.1



Ovarian ca. OVCAR-4
0.1



Ovarian ca. OVCAR-5
0.2



Ovarian ca. IGROV-1
0.1



Ovarian ca. OVCAR-8
0.1



Ovary
3.6



Breast ca. MCF-7
0.5



Breast ca. MDA-MB-231
0.1



Breast ca. BT 549
2.6



Breast ca. T47D
0.2



Breast ca. MDA-N
2.2



Breast Pool
0.1



Trachea
1.0



Lung
0.0



Fetal Lung
5.6



Lung ca. NCI-N417
0.0



Lung ca. LX-1
0.0



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.0



Lung ca. A549
0.0



Lung ca. NCI-H526
0.0



Lung ca. NCI-H23
0.3



Lung ca. NCI-H460
0.1



Lung ca. HOP-62
0.6



Lung ca. NCI-H522
0.0



Liver
0.0



Fetal Liver
1.3



Liver ca. HepG2
0.0



Kidney Pool
0.2



Fetal Kidney
1.4



Renal ca. 786-0
0.2



Renal ca. A498
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
4.6



Renal ca. TK-10
0.0



Bladder
0.6



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
0.0



Colon ca.* (SW480 met) SW620
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon cancer tissue
1.2



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
0.2



Small Intestine Pool
0.2



Stomach Pool
0.1



Bone Marrow Pool
0.2



Fetal Heart
1.0



Heart Pool
0.3



Lymph Node Pool
0.4



Fetal Skeletal Muscle
0.1



Skeletal Muscle Pool
0.2



Spleen Pool
1.1



Thymus Pool
0.6



CNS cancer (glio/astro) U87-MG
0.8



CNS cancer (glio/astro) U-118-MG
3.0



CNS cancer (neuro; met) SK-N-AS
0.0



CNS cancer (astro) SF-539
18.8



CNS cancer (astro) SNB-75
100.0



CNS cancer (glio) SNB-19
0.0



CNS cancer (glio) SF-295
0.8



Brain (Amygdala) Pool
0.0



Brain (cerebellum)
0.0



Brain (fetal)
0.0



Brain (Hippocampus) Pool
0.0



Cerebral Cortex Pool
0.0



Brain (Substantia nigra) Pool
0.0



Brain (Thalamus) Pool
0.0



Brain (whole)
0.0



Spinal Cord Pool
0.1



Adrenal Gland
0.1



Pituitary gland Pool
0.1



Salivary Gland
0.2



Thyroid (female)
0.1



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
0.4









Column A - Rel. Exp. (%) Ag3899, Run 219166475














TABLE AUD










HASS Panel v1.0










Tissue Name
A














MCF-7 C1
43.8



MCF-7 C2
51.4



MCF-7 C3
11.2



MCF-7 C4
72.2



MCF-7 C5
11.0



MCF-7 C6
43.8



MCF-7 C7
18.8



MCF-7 C9
17.3



MCF-7 C10
100.0



MCF-7 C11
3.2



MCF-7 C12
22.1



MCF-7 C13
26.4



MCF-7 C15
10.4



MCF-7 C16
45.1



MCF-7 C17
22.2



T24 D1
0.0



T24 D2
0.0



T24 D3
0.0



T24 D4
0.0



T24 D5
0.1



T24 D6
0.0



T24 D7
0.0



T24 D9
0.0



T24 D10
0.0



T24 D11
0.0



T24 D12
0.0



T24 D13
0.0



T24 D15
0.0



T24 D16
0.0



T24 D17
0.0



CAPaN B1
0.0



CAPaN B2
0.0



CAPaN B3
0.0



CAPaN B4
0.0



CAPaN B5
0.0



CAPaN B6
0.0



CAPaN B7
0.0



CAPaN B8
0.0



CAPaN B9
0.0



CAPaN B10
0.0



CAPaN B11
0.0



CAPaN B12
0.0



CAPaN B13
0.0



CAPaN B14
0.0



CAPaN B15
0.0



CAPaN B16
0.0



CAPaN B17
0.0



U87-MG F1 (B)
0.2



U87-MG F2
0.1



U87-MG F3
0.8



U87-MG F4
0.4



U87-MG F5
3.5



U87-MG F6
1.5



U87-MG F7
0.6



U87-MG F8
1.6



U87-MG F9
0.0



U87-MG F10
2.2



U87-MG F11
3.1



U87-MG F12
1.8



U87-MG F13
0.7



U87-MG F14
1.1



U87-MG F15
0.7



U87-MG F16
1.4



U87-MG F17
1.8



LnCAP A1
0.0



LnCAP A2
0.0



LnCAP A3
0.0



LnCAP A4
0.0



LnCAP A5
0.0



LnCAP A6
0.0



LnCAP A7
0.0



LnCAP A8
0.0



LnCAP A9
0.0



LnCAP A10
0.0



LnCAP A11
0.0



LnCAP A12
0.0



LnCAP A13
0.0



LnCAP A14
0.0



LnCAP A15
0.0



LnCAP A16
0.0



LnCAP A17
0.0



Primary Astrocytes
4.9



Primary Renal Proximal Tubule Epithelial cell A2
0.3



Primary melanocytes A5
0.4



126443 - 341 medullo
0.0



126444 - 487 medullo
0.0



126445 - 425 medullo
2.7



126446 - 690 medullo
0.5



126447 - 54 adult glioma
0.2



126448 - 245 adult glioma
38.4



126449 - 317 adult glioma
0.0



126450 - 212 glioma
0.0



126451 - 456 glioma
0.3









Column A - Rel. Exp. (%) Ag3108, Run 268623842














TABLE AUE










Panel 2.1










Tissue Name
A














Normal Colon
0.7



Colon cancer (OD06064)
1.3



Colon cancer margin (OD06064)
0.0



Colon cancer (OD06159)
0.5



Colon cancer margin (OD06159)
1.8



Colon cancer (OD06298-08)
1.6



Colon cancer margin (OD06298-018)
0.3



Colon Cancer Gr.2 ascend colon (ODO3921)
1.6



Colon Cancer margin (ODO3921)
4.6



Colon cancer metastasis (OD06104)
2.1



Lung margin (OD06104)
2.8



Colon mets to lung (OD04451-01)
4.5



Lung margin (OD04451-02)
10.7



Normal Prostate
0.8



Prostate Cancer (OD04410)
0.7



Prostate margin (OD04410)
13.6



Normal Lung
34.2



Invasive poor diff. lung adeno 1 (ODO4945-01)
9.2



Lung margin (ODO4945-03)
6.2



Lung Malignant Cancer (OD03126)
11.1



Lung margin (OD03126)
34.9



Lung Cancer (OD05014A)
25.2



Lung margin (OD05014B)
5.6



Lung Cancer (OD04237-01)
1.5



Lung margin (OD04237-02)
63.3



Ocular Mel Met to Liver (ODO4310)
24.3



Liver margin (ODO4310)
7.6



Melanoma Mets to Lung (OD04321)
100.0



Lung margin (OD04321)
20.2



Normal Kidney
3.6



Kidney Ca, Nuclear grade 2 (OD04338)
6.9



Kidney margin (OD04338)
2.1



Kidney Ca Nuclear grade 1/2 (OD04339)
1.1



Kidney margin (OD04339)
0.2



Kidney Ca, Clear cell type (OD04340)
8.8



Kidney margin (OD04340)
4.5



Kidney Ca, Nuclear grade 3 (OD04348)
1.3



Kidney margin (OD04348)
1.8



Kidney Cancer (OD04450-01)
0.6



Kidney margin (OD04450-03)
4.6



Kidney Cancer 8120613
0.3



Kidney margin 8120614
0.5



Kidney Cancer 9010320
0.9



Kidney margin 9010321
9.5



Kidney Cancer 8120607
0.6



Kidney margin 8120608
0.7



Normal Uterus
1.7



Uterus Cancer
1.2



Normal Thyroid
0.1



Thyroid Cancer
0.9



Thyroid Cancer A302152
1.2



Thyroid margin A302153
0.9



Normal Breast
12.4



Breast Cancer
0.9



Breast Cancer
4.3



Breast Cancer (OD04590-01)
0.6



Breast Cancer Mets (OD04590-03)
6.6



Breast Cancer Metastasis
2.1



Breast Cancer
3.3



Breast Cancer 9100266
4.6



Breast margin 9100265
1.5



Breast Cancer A209073
2.5



Breast margin A2090734
9.9



Normal Liver
4.2



Liver Cancer 1026
1.8



Liver Cancer 1025
6.1



Liver Cancer 6004-T
3.5



Liver Tissue 6004-N
0.8



Liver Cancer 6005-T
14.2



Liver Tissue 6005-N
14.8



Liver Cancer
1.4



Normal Bladder
1.7



Bladder Cancer
1.8



Bladder Cancer
2.4



Normal Ovary
7.7



Ovarian Cancer
13.6



Ovarian cancer (OD06145)
0.6



Ovarian cancer margin (OD06145)
2.2



Normal Stomach
4.1



Gastric Cancer 9060397
1.2



Stomach margin 9060396
0.5



Gastric Cancer 9060395
7.4



Stomach margin 9060394
2.6



Gastric Cancer 064005
4.3









Column A - Rel. Exp. (%) Ag3108, Run 170686074














TABLE AUF










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.3



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
3.2



HUVEC starved
8.1



HUVEC IL-1beta
4.1



HUVEC IFN gamma
15.8



HUVEC TNF alpha + IFN gamma
1.0



HUVEC TNF alpha + IL4
2.9



HUVEC IL-11
4.2



Lung Microvascular EC none
1.5



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.4



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
8.5



Coronery artery SMC TNFalpha + IL-1beta
1.8



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.5



KU-812 (Basophil) rest
1.0



KU-812 (Basophil) PMA/ionomycin
8.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
7.6



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
17.9



HPAEC TNF alpha + IL-1 beta
11.3



Lung fibroblast none
3.4



Lung fibroblast TNF alpha + IL-1 beta
2.7



Lung fibroblast IL-4
4.4



Lung fibroblast IL-9
2.2



Lung fibroblast IL-13
3.9



Lung fibroblast IFN gamma
7.2



Dermal fibroblast CCD1070 rest
5.5



Dermal fibroblast CCD1070 TNF alpha
1.9



Dermal fibroblast CCD1070 IL-1 beta
1.5



Dermal fibroblast IFN gamma
29.5



Dermal fibroblast IL-4
75.8



Dermal Fibroblasts rest
21.5



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.0



Colon
2.0



Lung
100.0



Thymus
0.5



Kidney
3.4









Column A - Rel. Exp. (%) Ag3899, Run 170120166














TABLE AUG










general oncology screening panel_v_2.4











Tissue Name
A
B















Colon cancer 1
13.9
10.8



Colon NAT 1
7.3
4.5



Colon cancer 2
2.3
5.2



Colon NAT 2
1.1
0.8



Colon cancer 3
14.1
11.7



Colon NAT 3
16.6
4.2



Colon malignant cancer 4
14.1
9.9



Colon NAT 4
0.3
0.3



Lung cancer 1
8.2
18.3



Lung NAT 1
0.0
0.4



Lung cancer 2
96.6
47.0



Lung NAT 2
1.4
2.4



Squamous cell carcinoma 3
50.0
22.2



Lung NAT 3
0.6
0.7



Metastatic melanoma 1
1.5
3.7



Melanoma 2
0.0
0.2



Melanoma 3
0.0
0.5



Metastatic melanoma 4
100.0
100.0



Metastatic melanoma 5
95.3
47.3



Bladder cancer 1
0.0
0.0



Bladder NAT 1
0.0
0.0



Bladder cancer 2
0.0
0.2



Bladder NAT 2
0.0
0.0



Bladder NAT 3
0.0
0.0



Bladder NAT 4
1.3
1.1



Prostate adenocarcinoma 1
1.2
7.2



Prostate adenocarcinoma 2
0.3
0.9



Prostate adenocarcinoma 3
1.3
0.8



Prostate adenocarcinoma 4
11.8
5.8



Prostate NAT 5
5.7
1.7



Prostate adenocarcinoma 6
0.3
0.8



Prostate adenocarcinoma 7
1.0
1.6



Prostate adenocarcinoma 8
0.4
0.7



Prostate adenocarcinoma 9
23.3
41.5



Prostate NAT 10
0.0
0.0



Kidney cancer 1
48.3
7.0



Kidney NAT 1
4.5
1.8



Kidney cancer 2
40.6
29.5



Kidney NAT 2
9.0
11.3



Kidney cancer 3
25.3
11.5



Kidney NAT 3
1.5
2.7



Kidney cancer 4
40.1
11.2



Kidney NAT 4
2.3
1.3









Column A - Rel. Exp. (%) Ag3108, Run 259737911






Column B - Rel. Exp. (%) Ag3899, Run 268143635







General_screening_panel_v1.4 Summary: Ag3899 Highest expression of this gene was detected in an astrocytoma SNB-75 cell line (CT=23.8). High levels of expression of this gene are also seen in a cluster of brain and melanoma cancer cell lines. Expression of this gene is useful as a marker to distinguish these samples from other samples in the panel and also as marker for detection of these cancers. Therapeutic modulation of the activity of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies, is beneficial in the treatment of these cancers.


Among tissues with metabolic or endocrine function, this gene was expressed at low to moderate levels in pancreas, adipose, adrenal gland, thyroid, pituitary gland, skeletal muscle, heart, liver and the gastrointestinal tract. Therapeutic modulation of the activity of this gene or its gene product is useful in the treatment of endocrine/metabolically related diseases, such as obesity and diabetes.


This gene was expressed at much higher levels in fetal liver (CT=30) and lung (CTs=27.9) when compared to corresponding adult liver (CT=35) and lung (CT=36.8). Expression of this gene or ists protein product is of use as a marker to distinguish these fetal tissues from the corresponding adult tissues.


HASS Panel v1.0 Summary: Ag3108 This gene was expressed by MCF-7 cells and a glioma sample on this panel. Expression of this gene was serum-dependent in MCF-7 cells. Expression is regulated by cytokines and extracellular molecules found in serum. Modulation of this gene, through the use of small molecule drugs, protein therapeutics or antibodies is of benefit in the treatment of glioma.


Panel 2.1 Summary: Ag3108 Highest expression of this gene was detected in a melanoma metastasis sample (CT=29). Expression of this gene was higher in normal liver when compared to adjancent cancerous tissue and in metastasic breast cancer tissue (OD04590-03) (CT=33) as compared to primary breast cancer (OD04590-01) (CT=36.7). Expression of this gene is useful as a marker for cancer metastasis. Therapeutic modulation of the expression or function of this gene or gene product is useful in the treatment of lung, breast and melanoma cancers.


Panel 4.1D Summary: Ag3108 Highest expression of this gene was seen in lung (CT=28.6). Significant expression of this gene was also seen in HPAEC cells, HUVEC cells, lung fibroblast, TNFalpha+IL1 beta treated bronchial epithelium and dermal fibroblasts. Antibody, protein therapeutics or small molecule therapies designed with the protein encoded for by this gene are important in the treatment of inflammatory lung disorders such as chronic obstructive pulmonary disease, asthma, allergy and emphysema and skin disorders including psoriasis.


In addition, low expression of this gene was also seen in kidney and colon. Antibody, protein therapeutics or small molecule therapies designed with the protein encoded by this gene are important in the treatment of inflammatory or autoimmune diseases that affect the kidney, including lupus and glomerulonephritis, as well as, inflammatory bowel diseases such as Crohns.


Expression of this gene was stimulated in PMA/ionomycin treated basophils (CT=30) as compared to resting basophils (CT=36). Basophils release histamines and other biological modifiers in reponse to allergens and play an important role in the pathology of asthma and hypersensitivity reactions. Therapeutics designed against the protein encoded by this gene are useful for the reduction or inhibition of inflammation by blocking basophil function in these diseases. These cells are a reasonable model for the inflammatory cells that take part in various inflammatory lung and bowel diseases, such as asthma, Crohn's disease, and ulcerative colitis. Therapeutics that modulate the function of this gene or gene product are useful for the reduction or elimination of the symptoms of patients suffering from asthma, Crohn's disease, and ulcerative colitis.


general oncology screening panel_V—2.4 Summary: Ag3108/Ag3960 Highest expression of this gene was seen in a metastatic melanoma sample (CTs=30-31). Expression of this gene was higher in kidney and lung cancer when compared to normal adjacent tissue samples. Expression of this gene is useful as a marker to differentiate these samples from other samples on this panel and as a marker for these cancers. Therapeutic modulation of the expression or function of this gene or gene product is of use in the treatment of these cancers.


AV. CG56959-02: Synaptotagmin X


Expression of full-length physical clone CG56959-02 was assessed using the primer-probe set Ag1557, described in Table AVA. Results of the RTQ-PCR runs are shown in Tables AVB, AVC, AVD and AVE.

TABLE AVAProbe Name Ag1557SEQStartIDPrimersSequencesLengthPositionNoForward5′-gcgtgcacagaaagactttaaa-3′226981519ProbeTET-5′-tgatgaaacttttcaatttcctgtagca-3′-286611520TAMRAReverse5′-tgaaatgtagttttcggttgct-3′226271521









TABLE AVB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
0.0



110980 COPD-F
0.0



110968 COPD-M
0.0



110977 COPD-M
0.0



110989 Emphysema-F
0.0



110992 Emphysema-F
0.0



110993 Emphysema-F
0.0



110994 Emphysema-F
0.0



110995 Emphysema-F
0.8



110996 Emphysema-F
0.0



110997 Asthma-M
2.6



111001 Asthma-F
0.0



111002 Asthma-F
0.0



111003 Atopic Asthma-F
0.0



111004 Atopic Asthma-F
0.0



111005 Atopic Asthma-F
0.0



111006 Atopic Asthma-F
0.0



111417 Allergy-M
0.0



112347 Allergy-M
2.4



112349 Normal Lung-F
2.4



112357 Normal Lung-F
2.1



112354 Normal Lung-M
0.0



112374 Crohns-F
23.8



112389 Match Control Crohns-F
7.0



112375 Crohns-F
32.1



112732 Match Control Crohns-F
0.0



112725 Crohns-M
11.7



112387 Match Control Crohns-M
0.0



112378 Crohns-M
5.0



112390 Match Control Crohns-M
1.3



112726 Crohns-M
26.1



112731 Match Control Crohns-M
67.8



112380 Ulcer Col-F
0.0



112734 Match Control Ulcer Col-F
0.0



112384 Ulcer Col-F
1.1



112737 Match Control Ulcer Col-F
12.9



112386 Ulcer Col-F
0.0



112738 Match Control Ulcer Col-F
0.9



112381 Ulcer Col-M
9.4



112735 Match Control Ulcer Col-M
62.4



112382 Ulcer Col-M
4.5



112394 Match Control Ulcer Col-M
0.0



112383 Ulcer Col-M
0.7



112736 Match Control Ulcer Col-M
2.1



112423 Psoriasis-F
1.6



112427 Match Control Psoriasis-F
2.7



112418 Psoriasis-M
0.0



112723 Match Control Psoriasis-M
4.4



112419 Psoriasis-M
5.8



112424 Match Control Psoriasis-M
2.3



112420 Psoriasis-M
1.3



112425 Match Control Psoriasis-M
0.0



104689 (MF) OA Bone-Backus
0.0



104690 (MF) Adj “Normal” Bone-Backus
0.8



104691 (MF) OA Synovium-Backus
0.0



104692 (BA) OA Cartilage-Backus
0.0



104694 (BA) OA Bone-Backus
0.8



104695 (BA) Adj “Normal” Bone-Backus
0.0



104696 (BA) OA Synovium-Backus
1.1



104700 (SS) OA Bone-Backus
0.0



104701 (SS) Adj “Normal” Bone-Backus
0.0



104702 (SS) OA Synovium-Backus
0.9



117093 OA Cartilage Rep7
0.0



112672 OA Bone5
9.4



112673 OA Synovium5
0.9



112674 OA Synovial Fluid cells5
2.8



117100 OA Cartilage Rep14
0.0



112756 OA Bone9
100.0



112757 OA Synovium9
0.0



112758 OA Synovial Fluid Cells9
0.0



117125 RA Cartilage Rep2
0.0



113492 Bone2 RA
0.0



113493 Synovium2 RA
0.0



113494 Syn Fluid Cells RA
0.6



113499 Cartilage4 RA
0.0



113500 Bone4 RA
0.0



113501 Synovium4 RA
1.6



113502 Syn Fluid Cells4 RA
0.0



113495 Cartilage3 RA
1.6



113496 Bone3 RA
0.0



113497 Synovium3 RA
2.1



113498 Syn Fluid Cells3 RA
0.0



117106 Normal Cartilage Rep20
0.0



113663 Bone3 Normal
6.5



113664 Synovium3 Normal
1.4



113665 Syn Fluid Cells3 Normal
5.8



117107 Normal Cartilage Rep22
0.0



113667 Bone4 Normal
0.0



113668 Synovium4 Normal
0.0



113669 Syn Fluid Cells4 Normal
0.0









Column A - Rel. Exp. (%) Ag1557, Run 257809393














TABLE AVC










Panel 1.3D











Tissue Name
A
B















Liver adenocarcinoma
0.0
0.0



Pancreas
0.0
4.9



Pancreatic ca. CAPAN 2
0.0
0.0



Adrenal gland
2.0
0.0



Thyroid
0.0
0.0



Salivary gland
0.0
0.0



Pituitary gland
68.3
60.7



Brain (fetal)
0.0
0.0



Brain (whole)
43.8
34.6



Brain (amygdala)
8.9
13.8



Brain (cerebellum)
0.0
0.0



Brain (hippocampus)
35.6
70.7



Brain (substantia nigra)
2.1
0.0



Brain (thalamus)
6.8
18.9



Cerebral Cortex
98.6
100.0



Spinal cord
21.5
13.2



glio/astro U87-MG
0.0
0.0



glio/astro U-118-MG
0.0
0.0



astrocytoma SW1783
0.0
0.0



neuro*; met SK-N-AS
0.0
0.0



astrocytoma SF-539
0.0
0.0



astrocytoma SNB-75
10.3
12.4



glioma SNB-19
0.0
0.0



glioma U251
1.6
0.0



glioma SF-295
0.0
0.0



Heart (fetal)
1.6
0.0



Heart
0.0
0.0



Skeletal muscle (fetal)
0.0
0.0



Skeletal muscle
0.0
0.0



Bone marrow
0.0
0.0



Thymus
0.0
0.0



Spleen
0.0
0.0



Lymph node
0.0
0.0



Colorectal
5.6
9.7



Stomach
15.5
12.9



Small intestine
22.2
50.3



Colon ca. SW480
0.0
0.0



Colon ca.* SW620(SW480 met)
0.0
0.0



Colon ca. HT29
0.0
0.0



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
3.7
2.4



Colon ca. tissue(ODO3866)
0.0
0.0



Colon ca. HCC-2998
0.0
0.0



Gastric ca.* (liver met) NCI-N87
0.0
0.0



Bladder
0.0
7.8



Trachea
2.6
3.8



Kidney
2.4
18.4



Kidney (fetal)
0.0
7.7



Renal ca. 786-0
2.3
0.0



Renal ca. A498
0.0
0.0



Renal ca. RXF 393
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.0
0.0



Liver
1.8
8.0



Liver (fetal)
0.0
0.0



Liver ca. (hepatoblast) HepG2
0.0
0.0



Lung
0.0
0.0



Lung (fetal)
0.0
0.0



Lung ca. (small cell) LX-1
0.0
0.0



Lung ca. (small cell) NCI-H69
0.0
0.0



Lung ca. (s. cell var.) SHP-77
0.0
0.0



Lung ca. (large cell)NCI-H460
0.0
0.0



Lung ca. (non-sm. cell) A549
0.0
0.0



Lung ca. (non-s. cell) NCI-H23
0.0
0.0



Lung ca. (non-s. cell) HOP-62
0.0
0.0



Lung ca. (non-s. cl) NCI-H522
1.9
5.9



Lung ca. (squam.) SW 900
5.3
0.0



Lung ca. (squam.) NCI-H596
0.0
0.0



Mammary gland
0.0
0.0



Breast ca.* (pl. ef) MCF-7
100.0
62.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0



Breast ca.* (pl. ef) T47D
0.0
0.0



Breast ca. BT-549
3.5
0.0



Breast ca. MDA-N
0.0
0.0



Ovary
0.0
9.7



Ovarian ca. OVCAR-3
41.5
23.7



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
0.0
0.0



Ovarian ca. OVCAR-8
0.0
0.0



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0
0.0



Uterus
4.2
4.6



Placenta
0.0
0.0



Prostate
7.2
11.2



Prostate ca.* (bone met)PC-3
0.0
0.0



Testis
13.3
11.1



Melanoma Hs688(A).T
0.0
0.0



Melanoma* (met) Hs688(B).T
0.0
0.0



Melanoma UACC-62
0.0
0.0



Melanoma M14
0.0
0.0



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0



Adipose
0.0
0.0









Column A - Rel. Exp. (%) Ag1557, Run 146380320






Column B - Rel. Exp. (%) Ag1557, Run 149871705














TABLE AVD










Panel 2D











Tissue Name
A
B















Normal Colon
22.5
51.4



CC Well to Mod Diff (ODO3866)
0.0
2.1



CC Margin (ODO3866)
9.0
17.6



CC Gr.2 rectosigmoid (ODO3868)
0.0
0.0



CC Margin (ODO3868)
3.7
10.3



CC Mod Diff (ODO3920)
0.0
0.0



CC Margin (ODO3920)
6.8
15.6



CC Gr.2 ascend colon (ODO3921)
9.6
11.8



CC Margin (ODO3921)
4.6
6.3



CC from Partial Hepatectomy
0.0
1.2



(ODO4309) Mets



Liver Margin (ODO4309)
2.3
1.2



Colon mets to lung (OD04451-01)
0.0
2.1



Lung Margin (OD04451-02)
0.0
0.0



Normal Prostate 6546-1
8.2
8.2



Prostate Cancer (OD04410)
2.3
5.8



Prostate Margin (OD04410)
13.8
16.6



Prostate Cancer (OD04720-01)
7.2
14.9



Prostate Margin (OD04720-02)
54.0
60.3



Normal Lung 061010
4.3
0.0



Lung Met to Muscle (ODO4286)
0.0
2.6



Muscle Margin (ODO4286)
0.0
0.0



Lung Malignant Cancer (OD03126)
0.0
0.0



Lung Margin (OD03126)
2.0
2.4



Lung Cancer (OD04404)
0.0
0.0



Lung Margin (OD04404)
0.0
0.0



Lung Cancer (OD04565)
0.0
0.0



Lung Margin (OD04565)
0.0
0.0



Lung Cancer (OD04237-01)
51.1
51.1



Lung Margin (OD04237-02)
0.0
1.4



Ocular Mel Met to Liver (ODO4310)
0.0
0.0



Liver Margin (ODO4310)
3.5
6.3



Melanoma Mets to Lung (OD04321)
0.0
0.0



Lung Margin (OD04321)
0.0
2.7



Normal Kidney
87.7
76.8



Kidney Ca, Nuclear grade 2 (OD04338)
4.7
8.8



Kidney Margin (OD04338)
18.9
19.2



Kidney Ca Nuclear grade 1/2 (OD04339)
14.7
14.6



Kidney Margin (OD04339)
14.5
13.3



Kidney Ca, Clear cell type (OD04340)
0.0
0.0



Kidney Margin (OD04340)
21.0
15.0



Kidney Ca, Nuclear grade 3 (OD04348)
0.0
0.0



Kidney Margin (OD04348)
13.1
22.4



Kidney Cancer (OD04622-01)
0.0
0.0



Kidney Margin (OD04622-03)
3.0
9.4



Kidney Cancer (OD04450-01)
0.0
0.0



Kidney Margin (OD04450-03)
20.4
31.4



Kidney Cancer 8120607
3.7
7.7



Kidney Margin 8120608
1.8
3.0



Kidney Cancer 8120613
100.0
100.0



Kidney Margin 8120614
2.0
1.4



Kidney Cancer 9010320
3.0
5.0



Kidney Margin 9010321
2.3
1.5



Normal Uterus
0.0
0.0



Uterus Cancer 064011
0.0
0.0



Normal Thyroid
0.0
0.0



Thyroid Cancer 064010
1.0
0.0



Thyroid Cancer A302152
0.0
0.0



Thyroid Margin A302153
0.0
0.0



Normal Breast
2.1
0.0



Breast Cancer (OD04566)
0.0
0.0



Breast Cancer (OD04590-01)
0.0
0.0



Breast Cancer Mets (OD04590-03)
1.4
0.0



Breast Cancer Metastasis (OD04655-05)
1.9
2.1



Breast Cancer 064006
41.2
0.0



Breast Cancer 1024
0.0
0.0



Breast Cancer 9100266
1.3
0.8



Breast Margin 9100265
0.0
0.0



Breast Cancer A209073
8.0
8.3



Breast Margin A209073
1.6
1.3



Normal Liver
8.5
15.0



Liver Cancer 064003
1.5
2.3



Liver Cancer 1025
3.4
6.4



Liver Cancer 1026
0.0
1.7



Liver Cancer 6004-T
7.1
2.0



Liver Tissue 6004-N
0.0
0.0



Liver Cancer 6005-T
0.0
2.0



Liver Tissue 6005-N
1.5
4.9



Normal Bladder
1.9
4.5



Bladder Cancer 1023
14.0
19.6



Bladder Cancer A302173
2.9
9.4



Bladder Cancer (OD04718-01)
50.7
74.2



Bladder Normal Adjacent (OD04718-03)
0.0
0.0



Normal Ovary
1.5
2.6



Ovarian Cancer 064008
1.2
0.0



Ovarian Cancer (OD04768-07)
0.0
0.0



Ovary Margin (OD04768-08)
0.0
0.0



Normal Stomach
13.8
15.3



Gastric Cancer 9060358
1.6
0.0



Stomach Margin 9060359
6.7
8.4



Gastric Cancer 9060395
1.4
1.2



Stomach Margin 9060394
2.5
9.0



Gastric Cancer 9060397
0.0
0.0



Stomach Margin 9060396
0.0
0.0



Gastric Cancer 064005
2.1
8.0









Column A - Rel. Exp. (%) Ag1557, Run 148394031






Column B - Rel. Exp. (%) Ag1557, Run 149871817














TABLE AVE










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
1.8



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.0



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.0



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
3.6



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
2.7



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
3.6



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
0.0



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
5.2



Lupus kidney
1.9



NCI-H292 none
1.6



NCI-H292 IL-4
1.6



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



IBD Colitis 2
7.3



IBD Crohn's
4.0



Colon
28.1



Lung
2.0



Thymus
100.0



Kidney
0.0









Column A - Rel. Exp. (%) Ag1557, Run 149871968







AI_comprehensive panel_v1.0 Summary: Ag1557 Low but significant expression was seen in an OA bone sample (CT=32). Prominent expression was also detected in ulcerative colitis and Crohn's samples as well as adjacent normal tissue samples.


Panel 1.3D Summary: Ag1557 Highest expression of this gene was detected in a breast cancer cell line and the cerebral cortex (CTs=33-34.6). Expression of this gene is of use as a marker for breast cancer. This gene was also expressed at moderate levels in the pituitary gland. The protein encoded by this gene is homologous to synaptotagmin and is important in the pituitary secretory pathway. This gene product or antibodies or small molecules that target this gene product are useful in the treatment of disease in this tissue.


Panel 2D Summary: Ag1557 Increased expression of this gene was seen in a single kidney sample (CTs=33), lung cancer sample and bladder cancer sample compared to the expression in the normal adjacent tissues showing that expression of this gene or gene product is of use as a marker to distinguish cancer from normal adjacent tissue. Therapeutic inhibition of this gene activity, through the use of small molecule drugs, protein therapeutics or antibodies, is of utility in the treatment of lung, bladder and kidney cancers.


Panel 4D Summary: Ag1557 This gene was expressed at a moderate level (CT=33.39) in thymus (Panel 4D). Small molecule drugs, protein therapeutics, or antibodies that inhibit the function of this synaptotagmin-like protein are useful for the reduction or elimination of the symptoms of autoimmune or inflammatory diseases that depend on the T cells that develop in the thymus, such as asthma, allergies, inflammatory bowel disease, lupus erythematosus, or rheumatoid arthritis.


AW. CG57111-01: Protocadherin


Expression of gene CG571 11-01 was assessed using the primer-probe sets Ag1096 and Ag3242, described in Tables AWA and AWB. Results of the RTQ-PCR runs are shown in Tables AWC, AWD, AWE, and AWF.

TABLE AWAProbe Name Ag1096SEQStartIDPrimersSequencesLengthPositionNoForward5′-actttggaagaggcattgct-3′2024031522ProbeTET-5′-cagacagattatgggctccatcgctt-3′-2624241523TAMRAReverse5′-ctcgggataaccatgatcact-3′2124631524









TABLE AWB










Probe Name Ag3242
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gggaccaatgctcaaattactt-3′
22
1038
1525






Probe
TET-5′-tcagaaagttccacaagcatctaagga-3′-
27
1070
1526



TAMRA





Reverse
5′-atgactccagtgttttcatcca-3′
22
1108
1527
















TABLE AWC










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
28.9



110980 COPD-F
6.7



110968 COPD-M
19.9



110977 COPD-M
8.4



110989 Emphysema-F
49.3



110992 Emphysema-F
22.8



110993 Emphysema-F
31.0



110994 Emphysema-F
12.7



110995 Emphysema-F
25.5



110996 Emphysema-F
15.8



110997 Asthma-M
7.5



111001 Asthma-F
30.4



111002 Asthma-F
45.4



111003 Atopic Asthma-F
45.1



111004 Atopic Asthma-F
22.4



111005 Atopic Asthma-F
25.3



111006 Atopic Asthma-F
13.9



111417 Allergy-M
15.1



112347 Allergy-M
5.0



112349 Normal Lung-F
1.9



112357 Normal Lung-F
5.1



112354 Normal Lung-M
11.6



112374 Crohns-F
32.5



112389 Match Control Crohns-F
14.4



112375 Crohns-F
36.1



112732 Match Control Crohns-F
0.0



112725 Crohns-M
45.4



112387 Match Control Crohns-M
18.3



112378 Crohns-M
3.8



112390 Match Control Crohns-M
22.4



112726 Crohns-M
45.7



112731 Match Control Crohns-M
12.6



112380 Ulcer Col-F
28.3



112734 Match Control Ulcer Col-F
0.8



112384 Ulcer Col-F
100.0



112737 Match Control Ulcer Col-F
28.1



112386 Ulcer Col-F
25.5



112738 Match Control Ulcer Col-F
2.1



112381 Ulcer Col-M
2.3



112735 Match Control Ulcer Col-M
53.6



112382 Ulcer Col-M
15.3



112394 Match Control Ulcer Col-M
10.2



112383 Ulcer Col-M
45.4



112736 Match Control Ulcer Col-M
4.0



112423 Psoriasis-F
90.1



112427 Match Control Psoriasis-F
55.9



112418 Psoriasis-M
25.7



112723 Match Control Psoriasis-M
0.0



112419 Psoriasis-M
66.0



112424 Match Control Psoriasis-M
42.0



112420 Psoriasis-M
44.4



112425 Match Control Psoriasis-M
24.3



104689 (MF) OA Bone-Backus
81.2



104690 (MF) Adj “Normal” Bone-Backus
16.6



104691 (MF) OA Synovium-Backus
1.5



104692 (BA) OA Cartilage-Backus
7.4



104694 (BA) OA Bone-Backus
56.3



104695 (BA) Adj “Normal” Bone-Backus
31.6



104696 (BA) OA Synovium-Backus
0.0



104700 (SS) OA Bone-Backus
63.7



104701 (SS) Adj “Normal” Bone-Backus
63.3



104702 (SS) OA Synovium-Backus
6.1



117093 OA Cartilage Rep7
16.3



112672 OA Bone5
13.2



112673 OA Synovium5
5.4



112674 OA Synovial Fluid cells5
7.7



117100 OA Cartilage Rep14
4.5



112756 OA Bone9
17.1



112757 OA Synovium9
21.8



112758 OA Synovial Fluid Cells9
13.3



117125 RA Cartilage Rep2
28.1



113492 Bone2 RA
34.2



113493 Synovium2 RA
8.9



113494 Syn Fluid Cells RA
25.7



113499 Cartilage4 RA
45.1



113500 Bone4 RA
47.3



113501 Synovium4 RA
42.6



113502 Syn Fluid Cells4 RA
25.5



113495 Cartilage3 RA
33.7



113496 Bone3 RA
38.7



113497 Synovium3 RA
18.2



113498 Syn Fluid Cells3 RA
30.8



117106 Normal Cartilage Rep20
0.0



113663 Bone3 Normal
2.8



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
2.9



117107 Normal Cartilage Rep22
10.5



113667 Bone4 Normal
17.2



113668 Synovium4 Normal
17.0



113669 Syn Fluid Cells4 Normal
36.3









Column A - Rel. Exp. (%) Ag3242, Run 253058919














TABLE AWD










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.0



Pancreas
1.0



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
0.3



Thyroid
0.0



Salivary gland
0.6



Pituitary gland
4.8



Brain (fetal)
11.8



Brain (whole)
40.6



Brain (amygdala)
31.6



Brain (cerebellum)
3.0



Brain (hippocampus)
76.3



Brain (substantia nigra)
2.1



Brain (thalamus)
6.3



Cerebral Cortex
27.7



Spinal cord
7.7



glio/astro U87-MG
0.6



glio/astro U-118-MG
0.5



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
7.5



astrocytoma SF-539
0.0



astrocytoma SNB-75
8.4



glioma SNB-19
3.0



glioma U251
66.4



glioma SF-295
0.6



Heart (fetal)
3.7



Heart
1.7



Skeletal muscle (fetal)
6.9



Skeletal muscle
1.9



Bone marrow
0.0



Thymus
0.4



Spleen
0.0



Lymph node
0.0



Colorectal
3.5



Stomach
2.5



Small intestine
7.2



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
0.0



Colon ca. HCC-2998
2.1



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.0



Trachea
9.0



Kidney
1.5



Kidney (fetal)
2.7



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.4



Renal ca. ACHN
100.0



Renal ca. UO-31
0.0



Renal ca. TK-10
1.1



Liver
1.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
4.8



Lung (fetal)
2.2



Lung ca. (small cell) LX-1
3.7



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
16.0



Lung ca. (large cell)NCI-H460
46.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
8.0



Lung ca. (non-s. cell) HOP-62
13.6



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
18.8



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
1.4



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
1.4



Breast ca. BT-549
1.6



Breast ca. MDA-N
0.0



Ovary
1.5



Ovarian ca. OVCAR-3
1.4



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
24.8



Ovarian ca. OVCAR-8
0.6



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
5.4



Uterus
15.5



Placenta
1.1



Prostate
1.4



Prostate ca.* (bone met)PC-3
0.4



Testis
2.3



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
1.6



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
58.6



Adipose
1.5









Column A - Rel. Exp. (%) Ag3242, Run 165524415














TABLE AWE










Panel 2.2










Tissue Name
A














Normal Colon
4.4



Colon cancer (OD06064)
1.8



Colon Margin (OD06064)
8.9



Colon cancer (OD06159)
2.0



Colon Margin (OD06159)
7.1



Colon cancer (OD06297-04)
2.4



Colon Margin (OD06297-05)
2.2



CC Gr.2 ascend colon (ODO3921)
0.9



CC Margin (ODO3921)
3.8



Colon cancer metastasis (OD06104)
0.7



Lung Margin (OD06104)
0.0



Colon mets to lung (OD04451-01)
1.7



Lung Margin (OD04451-02)
10.1



Normal Prostate
0.4



Prostate Cancer (OD04410)
0.3



Prostate Margin (OD04410)
0.0



Normal Ovary
0.0



Ovarian cancer (OD06283-03)
0.0



Ovarian Margin (OD06283-07)
0.0



Ovarian Cancer 064008
1.9



Ovarian cancer (OD06145)
0.0



Ovarian Margin (OD06145)
0.0



Ovarian cancer (OD06455-03)
0.0



Ovarian Margin (OD06455-07)
1.2



Normal Lung
2.3



Invasive poor diff. lung adeno (ODO4945-01
1.1



Lung Margin (ODO4945-03)
1.6



Lung Malignant Cancer (OD03126)
0.7



Lung Margin (OD03126)
1.2



Lung Cancer (OD05014A)
1.2



Lung Margin (OD05014B)
4.4



Lung cancer (OD06081)
0.8



Lung Margin (OD06081)
2.8



Lung Cancer (OD04237-01)
0.0



Lung Margin (OD04237-02)
6.0



Ocular Melanoma Metastasis
100.0



Ocular Melanoma Margin (Liver)
0.7



Melanoma Metastasis
1.2



Melanoma Margin (Lung)
4.9



Normal Kidney
0.0



Kidney Ca, Nuclear grade 2 (OD04338)
1.3



Kidney Margin (OD04338)
0.4



Kidney Ca Nuclear grade 1/2 (OD04339)
0.6



Kidney Margin (OD04339)
1.6



Kidney Ca, Clear cell type (OD04340)
0.0



Kidney Margin (OD04340)
1.1



Kidney Ca, Nuclear grade 3 (OD04348)
0.0



Kidney Margin (OD04348)
1.0



Kidney malignant cancer (OD06204B)
0.4



Kidney normal adjacent tissue (OD06204E)
1.8



Kidney Cancer (OD04450-01)
2.3



Kidney Margin (OD04450-03)
0.9



Kidney Cancer 8120613
0.0



Kidney Margin 8120614
0.4



Kidney Cancer 9010320
1.4



Kidney Margin 9010321
1.3



Kidney Cancer 8120607
0.8



Kidney Margin 8120608
0.2



Normal Uterus
5.3



Uterine Cancer 064011
0.5



Normal Thyroid
0.0



Thyroid Cancer 064010
0.7



Thyroid Cancer A302152
0.9



Thyroid Margin A302153
0.0



Normal Breast
0.6



Breast Cancer (OD04566)
0.0



Breast Cancer 1024
2.1



Breast Cancer (OD04590-01)
0.0



Breast Cancer Mets (OD04590-03)
0.0



Breast Cancer Metastasis (OD04655-05)
0.4



Breast Cancer 064006
0.7



Breast Cancer 9100266
0.7



Breast Margin 9100265
0.2



Breast Cancer A209073
0.0



Breast Margin A2090734
0.0



Breast cancer (OD06083)
0.4



Breast cancer node metastasis (OD06083)
0.9



Normal Liver
1.3



Liver Cancer 1026
0.3



Liver Cancer 1025
0.0



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
0.0



Liver Cancer 6005-T
2.7



Liver Tissue 6005-N
3.3



Liver Cancer 064003
7.1



Normal Bladder
0.0



Bladder Cancer 1023
0.0



Bladder Cancer A302173
0.3



Normal Stomach
5.8



Gastric Cancer 9060397
0.0



Stomach Margin 9060396
2.6



Gastric Cancer 9060395
2.2



Stomach Margin 9060394
3.4



Gastric Cancer 064005
0.3









Column A - Rel. Exp. (%) Ag3242, Run 174443348














TABLE AWF










Panel 4D











Tissue Name
A
B















Secondary Th1 act
0.0
0.0



Secondary Th2 act
0.0
0.0



Secondary Tr1 act
0.0
0.0



Secondary Th1 rest
0.0
0.0



Secondary Th2 rest
0.0
0.0



Secondary Tr1 rest
0.0
0.0



Primary Th1 act
0.0
0.0



Primary Th2 act
0.0
0.0



Primary Tr1 act
0.0
0.0



Primary Th1 rest
0.0
0.0



Primary Th2 rest
0.0
0.0



Primary Tr1 rest
0.0
0.0



CD45RA CD4 lymphocyte act
0.0
0.0



CD45RO CD4 lymphocyte act
0.0
0.0



CD8 lymphocyte act
1.3
0.0



Secondary CD8 lymphocyte rest
0.7
0.0



Secondary CD8 lymphocyte act
0.0
0.0



CD4 lymphocyte none
0.0
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0



LAK cells rest
0.0
0.0



LAK cells IL-2
0.0
0.0



LAK cells IL-2 + IL-12
0.0
0.0



LAK cells IL-2 + IFN gamma
0.8
0.0



LAK cells IL-2 + IL-18
0.0
0.0



LAK cells PMA/ionomycin
0.0
0.0



NK Cells IL-2 rest
0.8
0.0



Two Way MLR 3 day
0.0
0.0



Two Way MLR 5 day
0.0
0.0



Two Way MLR 7 day
1.5
0.0



PBMC rest
0.0
0.0



PBMC PWM
0.0
0.0



PBMC PHA-L
0.0
0.0



Ramos (B cell) none
0.0
0.0



Ramos (B cell) ionomycin
0.0
0.0



B lymphocytes PWM
0.0
0.0



B lymphocytes CD40L and IL-4
0.0
0.0



EOL-1 dbcAMP
0.0
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0
0.0



Dendritic cells none
0.0
0.0



Dendritic cells LPS
0.0
0.0



Dendritic cells anti-CD40
0.0
0.0



Monocytes rest
0.0
0.0



Monocytes LPS
1.7
0.0



Macrophages rest
0.0
0.0



Macrophages LPS
0.0
0.0



HUVEC none
0.0
0.0



HUVEC starved
0.0
0.0



HUVEC IL-1beta
0.0
0.0



HUVEC IFN gamma
0.4
0.0



HUVEC TNF alpha + IFN gamma
0.0
0.0



HUVEC TNF alpha + IL4
2.0
0.0



HUVEC IL-11
0.0
0.0



Lung Microvascular EC none
0.0
0.0



Lung Microvascular EC TNFalpha +
0.0
0.0



IL-1beta



Microvascular Dermal EC none
0.0
0.0



Microsvasular Dermal EC TNFalpha +
0.0
0.0



IL-1beta



Bronchial epithelium TNFalpha + IL1beta
0.0
0.0



Small airway epithelium none
0.0
0.0



Small airway epithelium TNFalpha +
6.5
11.7



IL-1beta



Coronery artery SMC rest
0.7
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0



Astrocytes rest
13.9
16.8



Astrocytes TNFalpha + IL-1beta
3.6
5.3



KU-812 (Basophil) rest
0.9
1.0



KU-812 (Basophil) PMA/ionomycin
100.0
100.0



CCD1106 (Keratinocytes) none
0.0
0.0



CCD1106 (Keratinocytes) TNFalpha +
0.0
0.0



IL-1beta



Liver cirrhosis
1.9
3.8



Lupus kidney
1.3
1.4



NCI-H292 none
42.9
48.0



NCI-H292 IL-4
14.7
14.8



NCI-H292 IL-9
40.6
36.6



NCI-H292 IL-13
12.2
7.4



NCI-H292 IFN gamma
16.8
10.9



HPAEC none
0.0
0.0



HPAEC TNF alpha + IL-1 beta
0.0
0.0



Lung fibroblast none
0.0
1.7



Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0



Lung fibroblast IL-4
1.7
0.0



Lung fibroblast IL-9
0.0
0.0



Lung fibroblast IL-13
0.7
0.0



Lung fibroblast IFN gamma
0.0
0.0



Dermal fibroblast CCD1070 rest
0.0
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.9
0.0



Dermal fibroblast IFN gamma
0.0
0.0



Dermal fibroblast IL-4
0.0
0.0



IBD Colitis 2
0.8
2.6



IBD Crohn's
0.0
2.0



Colon
28.1
23.2



Lung
34.9
51.4



Thymus
35.1
28.3



Kidney
8.2
7.4









Column A - Rel. Exp. (%) Ag1096, Run 160353275






Column B - Rel. Exp. (%) Ag3242, Run 164390548







AI_comprehensive panel_v1.0 Summary: Ag3242 Expression of this gene was ubiquitous in this panel, with high expression in samples derived from patients suffering from ulcerative colitis, Crohns disease and psoriasis (CTs=33). Significant expression was also seen in samples derived from synovium, cartilage and bone of rheumatoid arthritis. Antibody or small molecule therapies designed with the protein encoded for by this gene are useful in the treatment of inflammatory bowel diseases and rheumatoid arthritis.


Panel 1.3D Summary: Ag3242 Highest expression of the CG57448-01 gene was seen in a renal cancer cell line (CT=31.1). Significant expression was also seen in cell lines derived from ovarian cancer, lung cancer, brain cancer and melanoma. Expression of this gene is useful as a marker to differentiate between these samples and other samples on this panel and as a diagnostic marker for the presence of these cancers. This gene encodes a protein that is homologous to cadherin, a cell-adhesion molecule.


Therapeutic modulation of the expression or function of this gene or gene prduct is effective in the treatment of lung, renal and melanoma cancers.


Expression of the this gene was also high in many regions of the brain, including the amygdala, thalamus, cerebellum, and cerebral cortex, with highest expression in the hippocampus. Expression was also detected in the spinal cord. Cadherins can act as axon guidance and cell adhesion proteins, specifically during development and in the response to injury (Ranscht B. (2000) Int. J. Dev. Neurosci. 18: 643-651.). Manipulation of levels of this protein are of use in inducing a compensatory synaptogenic response to neuronal death in Alzheimer's disease, Parkinson's disease, Huntington's disease, spinocerebellar ataxia, progressive supranuclear palsy, ALS, head trauma, stroke, or any other disease/condition associated with neuronal loss.


Among tissues with metabolic function, this gene was moderately expressed in pituitary gland, adrenal gland, thyroid, pancreas, skeletal muscle, and liver, reflecting the widespread role of cadherins in cell-cell adhesion. This expression shows that this gene product plays a role in normal metabolic and neuroendocrine function and that dysregulated expression of this gene contributes to metabolic diseases (such as obesity and diabetes) or neuroendocrine disorders.


Panel 2.2 Summary: Ag3242 Highest expression of this gene was seen inma sample derived from an ocular melanoma metastasis (CT=29). Thus, expression of this gene is useful as a marker to differentiate between this sample and other samples on this panel.


Panel 4D Summary: Ag1096/Ag3242 Highest expression of this gene was detected in the basophil cell line (KU-812) treated with PMA/ionomycin (CTs=30-32). Significant expression was also seen in a cluster of treated and untreated samples derived from the muco-epidermoid cell line NCI-H292. Thus, this gene, which encodes a cadherin homolog, was expressed in both a cell line that is often used as a model of airway epithelium (NCI-H292) and a cell line that is a reasonable model for the inflammatory cells that contribute to various inflammatory lung diseases. Therapeutic modulation of this gene or gene prodcut may is useful to reduce or eliminate the symptoms of patients suffering from pathological and inflammatory lung disorders, including chronic obstructive pulmonary disease, asthma, allergy and emphysema.


Low but significant levels of expression were also seen in the samples derived from normal colon, kidney, lung and thymus. Therapeutic modulation of the expression or function of this gene or gene product is important for maintaining or restoring normal function to this organs during inflammation.


AX. CG5740905 and CG57409-07: Protein Containing MAM and Ig Domains


Expression of gene CG57409-05 and full length physical clone CG57409-07 was assessed using the primer-probe sets Ag5267 and Ag6188, described in Tables AXA and AXB. Results of the RTQ-PCR runs are shown in Tables AXC, AXD and AXE.

TABLE AXAProbe Name Ag5267SEQStartIDPrimersSequencesLengthPositionNoForward5′-gcggtcccggaaca-3′1411661528ProbeTET-5′-cacgcctggtctctcagtggca-3′-TAMRA2211971529Reverse5′-gcctgctgccacacatt-3′1712271530









TABLE AXB










Probe Name Ag6188
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ataaagtggtggtgatgcc-3′
19
1381
1531






Probe
TET-5′-agtggagccccctgccagtc-3′-TAMRA
20
1404
1532





Reverse
5′-gaggaagatggccatg-3′
16
1445
1533
















TABLE AXC










General_screening_panel_v1.5










Tissue Name
A














Adipose
1.5



Melanoma* Hs688(A).T
4.4



Melanoma* Hs688(B).T
7.7



Melanoma* M14
0.2



Melanoma* LOXIMVI
0.3



Melanoma* SK-MEL-5
9.2



Squamous cell carcinoma SCC-4
0.0



Testis Pool
0.7



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
2.0



Placenta
0.0



Uterus Pool
2.1



Ovarian ca. OVCAR-3
0.4



Ovarian ca. SK-OV-3
7.0



Ovarian ca. OVCAR-4
1.8



Ovarian ca. OVCAR-5
2.2



Ovarian ca. IGROV-1
0.5



Ovarian ca. OVCAR-8
0.7



Ovary
4.3



Breast ca. MCF-7
0.0



Breast ca. MDA-MB-231
3.5



Breast ca. BT 549
7.5



Breast ca. T47D
2.5



Breast ca. MDA-N
2.6



Breast Pool
5.7



Trachea
0.5



Lung
2.5



Fetal Lung
4.3



Lungca. NCI-N417
0.6



Lung ca. LX-1
3.6



Lung ca. NCI-H146
0.0



Lung ca. SHP-77
0.3



Lung ca. A549
2.6



Lung ca. NCI-H526
0.2



Lung ca. NCI-H23
0.1



Lung ca. NCI-H460
0.2



Lung ca. HOP-62
1.3



Lung ca. NCI-H522
19.3



Liver
0.1



Fetal Liver
0.3



Liver ca. HepG2
0.0



Kidney Pool
13.3



Fetal Kidney
1.2



Renal ca. 786-0
0.3



Renal ca. A498
5.2



Renal ca. ACHN
6.1



Renal ca. UO-31
2.5



Renal ca. TK-10
3.4



Bladder
1.0



Gastric ca. (liver met.) NCI-N87
0.0



Gastric ca. KATO III
0.0



Colon ca. SW-948
0.0



Colon ca. SW480
3.5



Colon ca.* (SW480 met) SW620
0.3



Colon ca. HT29
0.0



Colon ca. HCT-116
2.6



Colon ca. CaCo-2
0.1



Colon cancer tissue
0.9



Colon ca. SW1116
0.2



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
7.3



Small Intestine Pool
8.7



Stomach Pool
2.5



Bone Marrow Pool
3.6



Fetal Heart
1.5



Heart Pool
3.5



Lymph Node Pool
6.2



Fetal Skeletal Muscle
4.9



Skeletal Muscle Pool
1.0



Spleen Pool
2.1



Thymus Pool
2.9



CNS cancer (glio/astro) U87-MG
1.3



CNS cancer (glio/astro) U-118-MG
7.2



CNS cancer (neuro; met) SK-N-AS
0.3



CNS cancer (astro) SF-539
0.6



CNS cancer (astro) SNB-75
27.0



CNS cancer (glio) SNB-19
0.5



CNS cancer (glio) SF-295
4.2



Brain (Amygdala) Pool
2.3



Brain (cerebellum)
100.0



Brain (fetal)
34.4



Brain (Hippocampus) Pool
3.0



Cerebral Cortex Pool
3.5



Brain (Substantia nigra) Pool
3.2



Brain (Thalamus) Pool
2.7



Brain (whole)
5.1



Spinal Cord Pool
5.4



Adrenal Gland
4.0



Pituitary gland Pool
2.0



Salivary Gland
0.2



Thyroid (female)
0.3



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
2.5









Column A - Rel. Exp. (%) Ag6188, Run 257761488














TABLE AXD










Panel 4.1D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
4.3



Secondary Tr1 act
3.4



Secondary Th1 rest
0.6



Secondary Th2 rest
2.1



Secondary Tr1 rest
0.8



Primary Th1 act
0.0



Primary Th2 act
0.6



Primary Tr1 act
0.8



Primary Th1 rest
0.2



Primary Th2 rest
0.3



Primary Tr1 rest
0.2



CD45RA CD4 lymphocyte act
1.6



CD45RO CD4 lymphocyte act
3.4



CD8 lymphocyte act
0.4



Secondary CD8 lymphocyte rest
0.1



Secondary CD8 lymphocyte act
0.2



CD4 lymphocyte none
0.9



2ry Th1/Th2/Tr1_anti-CD95 CH11
1.8



LAK cells rest
6.2



LAK cells IL-2
3.3



LAK cells IL-2 + IL-12
1.2



LAK cells IL-2 + IFN gamma
0.7



LAK cells IL-2 + IL-18
0.6



LAK cells PMA/ionomycin
41.8



NK Cells IL-2 rest
9.0



Two Way MLR 3 day
2.9



Two Way MLR 5 day
3.5



Two Way MLR 7 day
2.7



PBMC rest
0.3



PBMC PWM
0.0



PBMC PHA-L
1.2



Ramos (B cell) none
0.0



Ramos (B cell) ionomycin
0.0



B lymphocytes PWM
0.7



B lymphocytes CD40L and IL-4
7.6



EOL-1 dbcAMP
7.7



EOL-1 dbcAMP PMA/ionomycin
100.0



Dendritic cells none
0.5



Dendritic cells LPS
0.4



Dendritic cells anti-CD40
0.4



Monocytes rest
0.0



Monocytes LPS
3.0



Macrophages rest
0.0



Macrophages LPS
0.7



HUVEC none
0.7



HUVEC starved
1.2



HUVEC IL-1beta
1.4



HUVEC IFN gamma
4.8



HUVEC TNF alpha + IFN gamma
3.8



HUVEC TNF alpha + IL4
0.4



HUVEC IL-11
0.4



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.4



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.2



Bronchial epithelium TNFalpha + IL1beta
0.7



Small airway epithelium none
0.2



Small airway epithelium TNFalpha + IL-1beta
1.6



Coronery artery SMC rest
0.9



Coronery artery SMC TNFalpha + IL-1beta
1.9



Astrocytes rest
8.8



Astrocytes TNFalpha + IL-1beta
11.0



KU-812 (Basophil) rest
1.4



KU-812 (Basophil) PMA/ionomycin
9.3



CCD1106 (Keratinocytes) none
4.8



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
1.2



Liver cirrhosis
2.0



NCI-H292 none
0.4



NCI-H292 IL-4
1.8



NCI-H292 IL-9
1.4



NCI-H292 IL-13
2.8



NCI-H292 IFN gamma
5.6



HPAEC none
0.2



HPAEC TNF alpha + IL-1 beta
4.9



Lung fibroblast none
46.7



Lung fibroblast TNF alpha + IL-1 beta
9.7



Lung fibroblast IL-4
23.5



Lung fibroblast IL-9
32.5



Lung fibroblast IL-13
18.6



Lung fibroblast IFN gamma
73.7



Dermal fibroblast CCD1070 rest
1.8



Dermal fibroblast CCD1070 TNF alpha
0.3



Dermal fibroblast CCD1070 IL-1 beta
2.0



Dermal fibroblast IFN gamma
11.7



Dermal fibroblast IL-4
4.0



Dermal Fibroblasts rest
10.1



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.7



Colon
0.6



Lung
0.7



Thymus
1.1



Kidney
2.4









Column A - Rel. Exp. (%) Ag5267, Run 230510063














TABLE AXE










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
6.4



Colon cancer NAT 1
0.0



Colon cancer 2
2.7



Colon cancer NAT 2
3.9



Colon cancer 3
7.0



Colon cancer NAT 3
11.5



Colon malignant cancer 4
5.5



Colon normal adjacent tissue 4
3.7



Lung cancer 1
7.7



Lung NAT 1
0.5



Lung cancer 2
12.3



Lung NAT 2
3.2



Squamous cell carcinoma 3
48.6



Lung NAT 3
4.5



metastatic melanoma 1
52.1



Melanoma 2
23.8



Melanoma 3
3.4



metastatic melanoma 4
97.9



metastatic melanoma 5
100.0



Bladder cancer 1
4.5



Bladder cancer NAT 1
0.0



Bladder cancer 2
19.5



Bladder cancer NAT 2
1.4



Bladder cancer NAT 3
0.0



Bladder cancer NAT 4
20.9



Prostate adenocarcinoma 1
69.3



Prostate adenocarcinoma 2
6.5



Prostate adenocarcinoma 3
17.7



Prostate adenocarcinoma 4
11.7



Prostate cancer NAT 5
5.0



Prostate adenocarcinoma 6
13.4



Prostate adenocarcinoma 7
10.3



Prostate adenocarcinoma 8
11.3



Prostate adenocarcinoma 9
93.3



Prostate cancer NAT 10
1.4



Kidney cancer 1
14.8



KidneyNAT 1
2.9



Kidney cancer 2
16.4



Kidney NAT 2
12.2



Kidney cancer 3
18.4



Kidney NAT 3
5.3



Kidney cancer 4
2.8



Kidney NAT 4
2.1









Column A - Rel. Exp. (%) Ag6188, Run 264979750







General_screening_panel_v1.5 Summary: Ag6188 Significant expression of this gene was seen in the brain. Expression of this gene is useful as a marker to differentiate between brain derived samples and other samples on this panel.


Among tissues with metabolic function, this gene was expressed at moderate to low levels in pituitary, adipose, adrenal gland, pancreas, thyroid, and adult and fetal skeletal muscle, heart, and liver. This widespread expression among these tissues shows that this gene product plays a role in normal neuroendocrine and metabolic function and that disregulated expression of this gene contributes to neuroendocrine disorders or metabolic diseases, such as obesity and diabetes.


Panel 4.1D Summary: Ag5267 This transcript was expressed in LAK cells and treatment of the LAK cells with PMA and ionomycin upregulated the expression of this transcript. This transcript was also induced in activated EOL cells and in fibroblasts. This gene encodes a putative NCAM, a type of cell surface protein often involved in cellular interaction, adhesion and signaling. Therapeutics designed with the protein encoded for this transcript are important in the treatment of diseases such as asthma, emphysema, psoriasis and arthritis.


general oncology screening panel_V—2.4 Summary: Ag6188 Highest expression of this gene was seen in two melanoma samples (CTs=31). Prominent expression was seen in a squamous cell carcinoma sample and two prostate adenocarcinoma samples. Targeting this gene or its protein product is useful in the treatment of these cancers.


AY. CG57448-01: Protocadherin 10


Expression of gene CG57448-01 was assessed using the primer-probe sets Ag1096, and Ag3242, described in Tables AYA and AYB. Results of the RTQ-PCR runs are shown in Tables AYC, AYD, AYE, AYF, and AYG.

TABLE AYAProbe Name Ag1096SEQStartIDPrimersSequencesLengthPositionNoForward5′-actttggaagaggcattgct-3′2024551534ProbeTET-5′-cagacagattatgggctccatcgctt-3′-2624761535TAMRAReverse5′-ctcgggataaccatgatcact-3′2125151536









TABLE AYB










Probe Name Ag3242
















SEQ






Start
ID


Primers
Sequences
Length
Position
No















Forward
5′-gggaccaatgctcaaattactt-3′
22
1090
1537






Probe
TET-5′-tcagaaagttccacaagcatctaagga-3′-
27
1122
1538



TAMRA





Reverse
5′-atgactccagtgttttcatcca-3′
22
1160
1539
















TABLE AYC










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
28.9



110980 COPD-F
6.7



110968 COPD-M
19.9



110977 COPD-M
8.4



110989 Emphysema-F
49.3



110992 Emphysema-F
22.8



110993 Emphysema-F
31.0



110994 Emphysema-F
12.7



110995 Emphysema-F
25.5



110996 Emphysema-F
15.8



110997 Asthma-M
7.5



111001 Asthma-F
30.4



111002 Asthma-F
45.4



111003 Atopic Asthma-F
45.1



111004 Atopic Asthma-F
22.4



111005 Atopic Asthma-F
25.3



111006 Atopic Asthma-F
13.9



111417 Allergy-M
15.1



112347 Allergy-M
5.0



112349 Normal Lung-F
1.9



112357 Normal Lung-F
5.1



112354 Normal Lung-M
11.6



112374 Crohns-F
32.5



112389 Match Control Crohns-F
14.4



112375 Crohns-F
36.1



112732 Match Control Crohns-F
0.0



112725 Crohns-M
45.4



112387 Match Control Crohns-M
18.3



112378 Crohns-M
3.8



112390 Match Control Crohns-M
22.4



112726 Crohns-M
45.7



112731 Match Control Crohns-M
12.6



112380 Ulcer Col-F
28.3



112734 Match Control Ulcer Col-F
0.8



112384 Ulcer Col-F
100.0



112737 Match Control Ulcer Col-F
28.1



112386 Ulcer Col-F
25.5



112738 Match Control Ulcer Col-F
2.1



112381 Ulcer Col-M
2.3



112735 Match Control Ulcer Col-M
53.6



112382 Ulcer Col-M
15.3



112394 Match Control Ulcer Col-M
10.2



112383 Ulcer Col-M
45.4



112736 Match Control Ulcer Col-M
4.0



112423 Psoriasis-F
90.1



112427 Match Control Psoriasis-F
55.9



112418 Psoriasis-M
25.7



112723 Match Control Psoriasis-M
0.0



112419 Psoriasis-M
66.0



112424 Match Control Psoriasis-M
42.0



112420 Psoriasis-M
44.4



112425 Match Control Psoriasis-M
24.3



104689 (MF) OA Bone-Backus
81.2



104690 (MF) Adj “Normal” Bone-Backus
16.6



104691 (MF) OA Synovium-Backus
1.5



104692 (BA) OA Cartilage-Backus
7.4



104694 (BA) OA Bone-Backus
56.3



104695 (BA) Adj “Normal” Bone-Backus
31.6



104696 (BA) OA Synovium-Backus
0.0



104700 (SS) OA Bone-Backus
63.7



104701 (SS) Adj “Normal” Bone-Backus
63.3



104702 (SS) OA Synovium-Backus
6.1



117093 OA Cartilage Rep7
16.3



112672 OA Bone5
13.2



112673 OA Synovium5
5.4



112674 OA Synovial Fluid cells5
7.7



117100 OA Cartilage Rep14
4.5



112756 OA Bone9
17.1



112757 OA Synovium9
21.8



112758 OA Synovial Fluid Cells9
13.3



117125 RA Cartilage Rep2
28.1



113492 Bone2 RA
34.2



113493 Synovium2 RA
8.9



113494 Syn Fluid Cells RA
25.7



113499 Cartilage4 RA
45.1



113500 Bone4 RA
47.3



113501 Synovium4 RA
42.6



113502 Syn Fluid Cells4 RA
25.5



113495 Cartilage3 RA
33.7



113496 Bone3 RA
38.7



113497 Synovium3 RA
18.2



113498 Syn Fluid Cells3 RA
30.8



117106 Normal Cartilage Rep20
0.0



113663 Bone3 Normal
2.8



113664 Synovium3 Normal
0.0



113665 Syn Fluid Cells3 Normal
2.9



117107 Normal Cartilage Rep22
10.5



113667 Bone4 Normal
17.2



113668 Synovium4 Normal
17.0



113669 Syn Fluid Cells4 Normal
36.3









Column A - Rel. Exp. (%) Ag3242, Run 253058919














TABLE AYD










CNS_neurodegeneration_v1.0











Tissue Name
A
B















AD 1 Hippo
74.2
45.4



AD 2 Hippo
18.8
27.5



AD 3 Hippo
32.3
19.5



AD 4 Hippo
6.8
6.3



AD 5 Hippo
39.2
30.8



AD 6 Hippo
100.0
100.0



Control 2 Hippo
89.5
61.6



Control 4 Hippo
13.6
10.1



Control (Path) 3 Hippo
17.6
3.8



AD 1 Temporal Ctx
8.2
6.2



AD 2 Temporal Ctx
19.8
11.8



AD 3 Temporal Ctx
9.9
4.0



AD 4 Temporal Ctx
12.1
6.3



AD 5 Inf Temporal Ctx
59.5
47.3



AD 5 Sup Temporal Ctx
72.7
67.8



AD 6 Inf Temporal Ctx
12.9
12.9



AD 6 Sup Temporal Ctx
14.6
12.2



Control 1 Temporal Ctx
7.5
8.5



Control 2 Temporal Ctx
34.4
35.1



Control 3 Temporal Ctx
13.9
10.8



Control 3 Temporal Ctx
5.2
2.9



Control (Path) 1 Temporal Ctx
38.7
32.8



Control (Path) 2 Temporal Ctx
25.7
18.3



Control (Path) 3 Temporal Ctx
4.1
6.0



Control (Path) 4 Temporal Ctx
18.4
7.6



AD 1 Occipital Ctx
14.8
7.0



AD 2 Occipital Ctx (Missing)
0.6
1.9



AD 3 Occipital Ctx
23.2
11.5



AD 4 Occipital Ctx
12.8
8.4



AD 5 Occipital Ctx
34.6
23.3



AD 6 Occipital Ctx
10.7
2.1



Control 1 Occipital Ctx
4.0
1.6



Control 2 Occipital Ctx
20.6
23.5



Control 3 Occipital Ctx
6.7
7.7



Control 4 Occipital Ctx
5.1
2.8



Control (Path) 1 Occipital Ctx
30.4
26.4



Control (Path) 2 Occipital Ctx
6.5
1.9



Control (Path) 3 Occipital Ctx
4.4
2.6



Control (Path) 4 Occipital Ctx
7.1
3.2



Control 1 Parietal Ctx
4.6
4.8



Control 2 Parietal Ctx
27.0
24.3



Control 3 Parietal Ctx
14.5
14.0



Control (Path) 1 Parietal Ctx
40.3
40.3



Control (Path) 2 Parietal Ctx
17.2
7.8



Control (Path) 3 Parietal Ctx
7.0
2.7



Control (Path) 4 Parietal Ctx
21.8
20.0









Column A - Rel. Exp. (%) Ag1096, Run 206231465






Column B - Rel. Exp. (%) Ag3242, Run 206533581














TABLE AYE










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
0.0



Pancreas
1.0



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
0.3



Thyroid
0.0



Salivary gland
0.6



Pituitary gland
4.8



Brain (fetal)
11.8



Brain (whole)
40.6



Brain (amygdala)
31.6



Brain (cerebellum)
3.0



Brain (hippocampus)
76.3



Brain (substantia nigra)
2.1



Brain (thalamus)
6.3



Cerebral Cortex
27.7



Spinal cord
7.7



glio/astro U87-MG
0.6



glio/astro U-118-MG
0.5



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
7.5



astrocytoma SF-539
0.0



astrocytoma SNB-75
8.4



glioma SNB-19
3.0



glioma U251
66.4



glioma SF-295
0.6



Heart (fetal)
3.7



Heart
1.7



Skeletal muscle (fetal)
6.9



Skeletal muscle
1.9



Bone marrow
0.0



Thymus
0.4



Spleen
0.0



Lymph node
0.0



Colorectal
3.5



Stomach
2.5



Small intestine
7.2



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
0.0



Colon ca. HCC-2998
2.1



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
0.0



Trachea
9.0



Kidney
1.5



Kidney (fetal)
2.7



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.4



Renal ca. ACHN
100.0



Renal ca. UO-31
0.0



Renal ca. TK-10
1.1



Liver
1.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
4.8



Lung (fetal)
2.2



Lung ca. (small cell) LX-1
3.7



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
16.0



Lung ca. (large cell)NCI-H460
46.0



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
8.0



Lung ca. (non-s. cell) HOP-62
13.6



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
18.8



Lung ca. (squam.) NCI-H596
0.0



Mammary gland
1.4



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
1.4



Breast ca. BT-549
1.6



Breast ca. MDA-N
0.0



Ovary
1.5



Ovarian ca. OVCAR-3
1.4



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
24.8



Ovarian ca. OVCAR-8
0.6



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
5.4



Uterus
15.5



Placenta
1.1



Prostate
1.4



Prostate ca.* (bone met)PC-3
0.4



Testis
2.3



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
0.0



Melanoma M14
1.6



Melanoma LOX IMVI
0.0



Melanoma* (met) SK-MEL-5
58.6



Adipose
1.5









Column A - Rel. Exp. (%) Ag3242, Run 165524415














TABLE AYF










Panel 2.2










Tissue Name
A














Normal Colon
4.4



Colon cancer (OD06064)
1.8



Colon Margin (OD06064)
8.9



Colon cancer (OD06159)
2.0



Colon Margin (OD06159)
7.1



Colon cancer (OD06297-04)
2.4



Colon Margin (OD06297-05)
2.2



CC Gr.2 ascend colon (ODO3921)
0.9



CC Margin (ODO3921)
3.8



Colon cancer metastasis (OD06104)
0.7



Lung Margin (OD06104)
0.0



Colon mets to lung (OD04451-01)
1.7



Lung Margin (OD04451-02)
10.1



Normal Prostate
0.4



Prostate Cancer (OD04410)
0.3



Prostate Margin (OD04410)
0.0



Normal Ovary
0.0



Ovarian cancer (OD06283-03)
0.0



Ovarian Margin (OD06283-07)
0.0



Ovarian Cancer 064008
1.9



Ovarian cancer (OD06145)
0.0



Ovarian Margin (OD06145)
0.0



Ovarian cancer (OD06455-03)
0.0



Ovarian Margin (OD06455-07)
1.2



Normal Lung
2.3



Invasive poor diff. lung adeno (ODO4945-01
1.1



Lung Margin (ODO4945-03)
1.6



Lung Malignant Cancer (OD03126)
0.7



Lung Margin (OD03126)
1.2



Lung Cancer (OD05014A)
1.2



Lung Margin (OD05014B)
4.4



Lung cancer (OD06081)
0.8



Lung Margin (OD06081)
2.8



Lung Cancer (OD04237-01)
0.0



Lung Margin (OD04237-02)
6.0



Ocular Melanoma Metastasis
100.0



Ocular Melanoma Margin (Liver)
0.7



Melanoma Metastasis
1.2



Melanoma Margin (Lung)
4.9



Normal Kidney
0.0



Kidney Ca, Nuclear grade 2 (OD04338)
1.3



Kidney Margin (OD04338)
0.4



Kidney Ca Nuclear grade 1/2 (OD04339)
0.6



Kidney Margin (OD04339)
1.6



Kidney Ca, Clear cell type (OD04340)
0.0



Kidney Margin (OD04340)
1.1



Kidney Ca, Nuclear grade 3 (OD04348)
0.0



Kidney Margin (OD04348)
1.0



Kidney malignant cancer (OD06204B)
0.4



Kidney normal adjacent tissue (OD06204E)
1.8



Kidney Cancer (OD04450-01)
2.3



Kidney Margin (OD04450-03)
0.9



Kidney Cancer 8120613
0.0



Kidney Margin 8120614
0.4



Kidney Cancer 9010320
1.4



Kidney Margin 9010321
1.3



Kidney Cancer 8120607
0.8



Kidney Margin 8120608
0.2



Normal Uterus
5.3



Uterine Cancer 064011
0.5



Normal Thyroid
0.0



Thyroid Cancer 064010
0.7



Thyroid Cancer A302152
0.9



Thyroid Margin A302153
0.0



Normal Breast
0.6



Breast Cancer (OD04566)
0.0



Breast Cancer 1024
2.1



Breast Cancer (OD04590-01)
0.0



Breast Cancer Mets (OD04590-03)
0.0



Breast Cancer Metastasis (OD04655-05)
0.4



Breast Cancer 064006
0.7



Breast Cancer 9100266
0.7



Breast Margin 9100265
0.2



Breast Cancer A209073
0.0



Breast Margin A2090734
0.0



Breast cancer (OD06083)
0.4



Breast cancer node metastasis (OD06083)
0.9



Normal Liver
1.3



Liver Cancer 1026
0.3



Liver Cancer 1025
0.0



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
0.0



Liver Cancer 6005-T
2.7



Liver Tissue 6005-N
3.3



Liver Cancer 064003
7.1



Normal Bladder
0.0



Bladder Cancer 1023
0.0



Bladder Cancer A302173
0.3



Normal Stomach
5.8



Gastric Cancer 9060397
0.0



Stomach Margin 9060396
2.6



Gastric Cancer 9060395
2.2



Stomach Margin 9060394
3.4



Gastric Cancer 064005
0.3









Column A - Rel. Exp. (%) Ag3242, Run 174443348














TABLE AYG










Panel 4D











Tissue Name
A
B















Secondary Th1 act
0.0
0.0



Secondary Th2 act
0.0
0.0



Secondary Tr1 act
0.0
0.0



Secondary Th1 rest
0.0
0.0



Secondary Th2 rest
0.0
0.0



Secondary Tr1 rest
0.0
0.0



Primary Th1 act
0.0
0.0



Primary Th2 act
0.0
0.0



Primary Tr1 act
0.0
0.0



Primary Th1 rest
0.0
0.0



Primary Th2 rest
0.0
0.0



Primary Tr1 rest
0.0
0.0



CD45RA CD4 lymphocyte act
0.0
0.0



CD45RO CD4 lymphocyte act
0.0
0.0



CD8 lymphocyte act
1.3
0.0



Secondary CD8 lymphocyte rest
0.7
0.0



Secondary CD8 lymphocyte act
0.0
0.0



CD4 lymphocyte none
0.0
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0



LAK cells rest
0.0
0.0



LAK cells IL-2
0.0
0.0



LAK cells IL-2 + IL-12
0.0
0.0



LAK cells IL-2 + IFN gamma
0.8
0.0



LAK cells IL-2 + IL-18
0.0
0.0



LAK cells PMA/ionomycin
0.0
0.0



NK Cells IL-2 rest
0.8
0.0



Two Way MLR 3 day
0.0
0.0



Two Way MLR 5 day
0.0
0.0



Two Way MLR 7 day
1.5
0.0



PBMC rest
0.0
0.0



PBMC PWM
0.0
0.0



PBMC PHA-L
0.0
0.0



Ramos (B cell) none
0.0
0.0



Ramos (B cell) ionomycin
0.0
0.0



B lymphocytes PWM
0.0
0.0



B lymphocytes CD40L and IL-4
0.0
0.0



EOL-1 dbcAMP
0.0
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0
0.0



Dendritic cells none
0.0
0.0



Dendritic cells LPS
0.0
0.0



Dendritic cells anti-CD40
0.0
0.0



Monocytes rest
0.0
0.0



Monocytes LPS
1.7
0.0



Macrophages rest
0.0
0.0



Macrophages LPS
0.0
0.0



HUVEC none
0.0
0.0



HUVEC starved
0.0
0.0



HUVEC IL-1beta
0.0
0.0



HUVEC IFN gamma
0.4
0.0



HUVEC TNF alpha + IFN gamma
0.0
0.0



HUVEC TNF alpha + IL4
2.0
0.0



HUVEC IL-11
0.0
0.0



Lung Microvascular EC none
0.0
0.0



Lung Microvascular EC TNFalpha +
0.0
0.0



IL-1beta



Microvascular Dermal EC none
0.0
0.0



Microsvasular Dermal EC TNFalpha +
0.0
0.0



IL-1beta



Bronchial epithelium TNFalpha + IL1beta
0.0
0.0



Small airway epithelium none
0.0
0.0



Small airway epithelium TNFalpha +
6.5
11.7



IL-1beta



Coronery artery SMC rest
0.7
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0
0.0



Astrocytes rest
13.9
16.8



Astrocytes TNFalpha + IL-1beta
3.6
5.3



KU-812 (Basophil) rest
0.9
1.0



KU-812 (Basophil) PMA/ionomycin
100.0
100.0



CCD1106 (Keratinocytes) none
0.0
0.0



CCD1106 (Keratinocytes) TNFalpha +
0.0
0.0



IL-1beta



Liver cirrhosis
1.9
3.8



Lupus kidney
1.3
1.4



NCI-H292 none
42.9
48.0



NCI-H292 IL-4
14.7
14.8



NCI-H292 IL-9
40.6
36.6



NCI-H292 IL-13
12.2
7.4



NCI-H292 IFN gamma
16.8
10.9



HPAEC none
0.0
0.0



HPAEC TNF alpha + IL-1 beta
0.0
0.0



Lung fibroblast none
0.0
1.7



Lung fibroblast TNF alpha + IL-1 beta
0.0
0.0



Lung fibroblast IL-4
1.7
0.0



Lung fibroblast IL-9
0.0
0.0



Lung fibroblast IL-13
0.7
0.0



Lung fibroblast IFN gamma
0.0
0.0



Dermal fibroblast CCD1070 rest
0.0
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.9
0.0



Dermal fibroblast IFN gamma
0.0
0.0



Dermal fibroblast IL-4
0.0
0.0



IBD Colitis 2
0.8
2.6



IBD Crohn's
0.0
2.0



Colon
28.1
23.2



Lung
34.9
51.4



Thymus
35.1
28.3



Kidney
8.2
7.4









Column A - Rel. Exp. (%) Ag1096, Run 160353275






Column B - Pel. Exp. (%) Ag3242, Run 164390548







AI_comprehensive panel_v1.0 Summary: Ag3242 Expression of this gene was ubiquitous in this panel, with high expression in samples derived from patients suffering from ulcerative colitis, Crohns disease and psoriasis (CTs=33). Significant expression was also seen in samples derived from synovium, cartilage and bone of rheumatoid arthritis. Antibody or small molecule therapies designed with the protein encoded for by this gene are useful in the treatment of inflammatory bowel diseases and rheumatoid arthritis.


Panel 1.3D Summary: Ag3242 Highest expression of the CG57448-01 gene was seen in a renal cancer cell line (CT=31.1). Significant expression was also seen in cell lines derived from ovarian cancer, lung cancer, brain cancer and melanoma. Expression of this gene is useful as a marker to differentiate between these samples and other samples on this panel and as a diagnostic marker for the presence of these cancers. This gene encodes a protein that is homologous to cadherin, a cell-adhesion molecule. Therapeutic modulation of the expression or function of this gene or gene prduct is effective in the treatment of lung, renal and melanoma cancers.


Expression of the this gene was also high in many regions of the brain, including the amygdala, thalamus, cerebellum, and cerebral cortex, with highest expression in the hippocampus. Expression was also detected in the spinal cord. Cadherins can act as axon guidance and cell adhesion proteins, specifically during development and in the response to injury (Ranscht B. (2000) Int. J. Dev. Neurosci. 18: 643-651.). Manipulation of levels of this protein are of use in inducing a compensatory synaptogenic response to neuronal death in Alzheimer's disease, Parkinson's disease, Huntington's disease, spinocerebellar ataxia, progressive supranuclear palsy, ALS, head trauma, stroke, or any other disease/condition associated with neuronal loss.


Among tissues with metabolic function, this gene was moderately expressed in pituitary gland, adrenal gland, thyroid, pancreas, skeletal muscle, and liver, reflecting the widespread role of cadherins in cell-cell adhesion. This expression shows that this gene product plays a role in normal metabolic and neuroendocrine function and that dysregulated expression of this gene contributes to metabolic diseases (such as obesity and diabetes) or neuroendocrine disorders.


Panel 2.2 Summary: Ag3242 Highest expression of this gene was seen in a sample derived from an ocular melanoma metastasis (CT=29). Thus, expression of this gene is useful as a marker to differentiate between this sample and other samples on this panel.


Panel 4D Summary: Ag1096/Ag3242 Highest expression of this gene was detected in the basophil cell line (KU-812) treated with PMA/ionomycin (CTs=30-32).


Significant expression was also seen in a cluster of treated and untreated samples derived from the muco-epidermoid cell line NCI-H292. Thus, this gene, which encodes a cadherin homolog, was expressed in both a cell line that is often used as a model of airway epithelium (NCI-H292) and a cell line that is a reasonable model for the inflammatory cells that contribute to various inflammatory lung diseases. Therapeutic modulation of this gene or gene prodcut may is useful to reduce or eliminate the symptoms of patients suffering from pathological and inflammatory lung disorders, including chronic obstructive pulmonary disease, asthma, allergy and emphysema.


Low but significant levels of expression were also seen in the samples derived from normal colon, kidney, lung and thymus. Therapeutic modulation of the expression or function of this gene or gene product is important for maintaining or restoring normal function to this organs during inflammation.


AZ. CG58567-01: Protocadherin


Expression of gene CG58567-01 was assessed using the primer-probe set Ag2897, described in Table AZA. Results of the RTQ-PCR runs are shown in Tables AZB, AZC, AZD, AZE and AZF.

TABLE AZAProbe Name Ag2897SEQStartIDPrimersSequencesLengthPositionNoForward5′-tgacaggggatattcatgctaa-3′221565`1540ProbeTET-5′-tggcaataaatactgcctcacagtcca-3′-2716081541TAMRAReverse5′-agttgcatcacctttgtctttg-3′2216381542









TABLE AZB










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
4.7



110980 COPD-F
22.5



110968 COPD-M
8.5



110977 COPD-M
51.8



110989 Emphysema-F
51.8



110992 Emphysema-F
5.0



110993 Emphysema-F
5.6



110994 Emphysema-F
3.3



110995 Emphysema-F
13.2



110996 Emphysema-F
0.6



110997 Asthma-M
1.4



111001 Asthma-F
8.1



111002 Asthma-F
12.5



111003 Atopic Asthma-F
12.8



111004 Atopic Asthma-F
12.3



111005 Atopic Asthma-F
6.1



111006 Atopic Asthma-F
3.7



111417 Allergy-M
7.7



112347 Allergy-M
0.8



112349 Normal Lung-F
0.8



112357 Normal Lung-F
20.2



112354 Normal Lung-M
2.1



112374 Crohns-F
4.7



112389 Match Control Crohns-F
2.1



112375 Crohns-F
6.7



112732 Match Control Crohns-F
2.2



112725 Crohns-M
18.9



112387 Match Control Crohns-M
11.2



112378 Crohns-M
1.3



112390 Match Control Crohns-M
62.4



112726 Crohns-M
9.6



112731 Match Control Crohns-M
16.0



112380 Ulcer Col-F
21.2



112734 Match Control Ulcer Col-F
4.5



112384 Ulcer Col-F
17.3



112737 Match Control Ulcer Col-F
5.2



112386 Ulcer Col-F
5.3



112738 Match Control Ulcer Col-F
2.7



112381 Ulcer Col-M
0.3



112735 Match Control Ulcer Col-M
23.7



112382 Ulcer Col-M
5.8



112394 Match Control Ulcer Col-M
6.5



112383 Ulcer Col-M
1.6



112736 Match Control Ulcer Col-M
1.3



112423 Psoriasis-F
7.9



112427 Match Control Psoriasis-F
100.0



112418 Psoriasis-M
2.8



112723 Match Control Psoriasis-M
14.3



112419 Psoriasis-M
10.7



112424 Match Control Psoriasis-M
8.7



112420 Psoriasis-M
40.1



112425 Match Control Psoriasis-M
65.1



104689 (MF) OA Bone-Backus
1.7



104690 (MF) Adj “Normal” Bone-Backus
4.5



104691 (MF) OA Synovium-Backus
9.5



104692 (BA) OA Cartilage-Backus
3.1



104694 (BA) OA Bone-Backus
1.3



104695 (BA) Adj “Normal” Bone-Backus
1.8



104696 (BA) OA Synovium-Backus
5.0



104700 (SS) OA Bone-Backus
5.2



104701 (SS) Adj “Normal” Bone-Backus
7.0



104702 (SS) OA Synovium-Backus
12.7



117093 OA Cartilage Rep7
15.1



112672 OA Bone5
15.9



112673 OA Synovium5
8.2



112674 OA Synovia Fluid cells5
7.9



117100 OA Cartilage Rep14
1.3



112756 OA Bone9
0.8



112757 OA Synovium9
0.4



112758 OA Synovial Fluid Cells9
10.2



117125 RA Cartilage Rep2
6.5



113492 Bone2 RA
2.2



113493 Synovium2 RA
0.2



113494 Syn Fluid Cells RA
2.0



113499 Cartilage4 RA
1.6



113500 Bone4 RA
1.3



113501 Synovium4 RA
1.9



113502 Syn Fluid Cells4 RA
1.0



113495 Cartilage3 RA
1.1



113496 Bone3 RA
1.4



113497 Synovium3 RA
0.2



113498 Syn Fluid Cells3 RA
1.2



117106 Normal Cartilage Rep20
0.3



113663 Bone3 Normal
2.8



113664 Synovium3 Normal
0.2



113665 Syn Fluid Cells3 Normal
1.3



117107 Normal Cartilage Rep22
9.2



113667 Bone4 Normal
9.5



113668 Synovium4 Normal
6.1



113669 Syn Fluid Cells4 Normal
10.2









Column A - Rel. Exp. (%) Ag2897, Run 229662505














TABLE AZC










Panel 1.3D











Tissue Name
A
B















Liver adenocarcinoma
0.0
0.0



Pancreas
1.4
2.4



Pancreatic ca. CAPAN 2
0.0
0.0



Adrenal gland
1.4
0.0



Thyroid
0.0
0.0



Salivary gland
0.0
0.0



Pituitary gland
4.1
9.0



Brain (fetal)
7.1
17.8



Brain (whole)
21.0
59.0



Brain (amygdala)
24.7
37.9



Brain (cerebellum)
5.6
28.5



Brain (hippocampus)
25.2
25.3



Brain (substantia nigra)
0.9
17.1



Brain (thalamus)
4.1
23.0



Cerebral Cortex
100.0
22.2



Spinal cord
68.8
100.0



glio/astro U87-MG
54.3
10.4



glio/astro U-118-MG
1.5
20.4



astrocytoma SW 1783
16.3
0.0



neuro*; met SK-N-AS
0.0
0.0



astrocytoma SF-539
0.0
4.3



astrocytoma SNB-75
18.4
28.7



glioma SNB-19
0.0
2.6



glioma U251
3.1
27.0



glioma SF-295
4.8
2.2



Heart (fetal)
0.0
0.0



Heart
1.4
0.0



Skeletal muscle (fetal)
77.4
0.0



Skeletal muscle
1.1
14.1



Bone marrow
0.0
2.5



Thymus
2.1
2.4



Spleen
0.0
4.4



Lymph node
0.0
4.8



Colorectal
10.7
0.0



Stomach
8.7
64.6



Small intestine
7.2
18.9



Colon ca. SW480
0.0
0.0



Colon ca.* SW620(SW480 met)
0.0
0.0



Colon ca. HT29
0.0
0.0



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
0.0
0.0



Colon ca. tissue(ODO3866)
1.7
8.1



Colon ca. HCC-2998
0.0
0.0



Gastric ca.* (liver met) NCI-N87
10.3
7.7



Bladder
14.2
29.3



Trachea
4.1
0.0



Kidney
0.0
0.0



Kidney (fetal)
2.8
2.4



Renal ca. 786-0
0.9
3.3



Renal ca. A498
13.9
23.7



Renal ca. RXF 393
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.0
0.0



Liver
0.0
2.9



Liver (fetal)
0.4
0.0



Liver ca. (hepatoblast) HepG2
0.0
0.0



Lung
1.4
0.0



Lung (fetal)
6.8
11.0



Lung ca. (small cell) LX-1
0.0
0.0



Lung ca. (small cell) NCI-H69
0.0
4.1



Lung ca. (s. cell var.) SHP-77
1.0
0.0



Lung ca. (large cell)NCI-H460
0.0
0.0



Lung ca. (non-sm. cell) A549
0.0
0.0



Lung ca. (non-s. cell) NCI-H23
0.0
3.0



Lung ca. (non-s. cell) HOP-62
0.6
7.5



Lung ca. (non-s. cl) NCI-H522
0.0
0.0



Lung ca. (squam.) SW 900
4.6
8.6



Lung ca. (squam.) NCI-H596
0.0
0.0



Mammary gland
7.9
17.1



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
14.5



Breast ca.* (pl. ef) T47D
0.0
0.0



Breast ca. BT-549
0.0
12.2



Breast ca. MDA-N
0.0
0.0



Ovary
12.1
6.1



Ovarian ca. OVCAR-3
3.8
12.1



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
32.5
25.5



Ovarian ca. OVCAR-8
1.9
0.0



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca.* (ascites) SK-OV-3
0.0
0.0



Uterus
12.4
66.4



Placenta
0.0
2.3



Prostate
0.0
0.0



Prostate ca.* (bone met)PC-3
0.0
0.0



Testis
56.3
68.8



Melanoma Hs688(A).T
1.3
8.2



Melanoma* (met) Hs688(B).T
2.5
4.8



Melanoma UACC-62
1.5
2.8



Melanoma M14
0.0
0.0



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0



Adipose
7.2
0.0









Column A - Rel. Exp. (%) Ag2897, Run 161905860






Column B - Rel. Exp. (%) Ag2897, Run 165518166














TABLE AZD










Panel 2D











Tissue Name
A
B















Normal Colon
83.5
90.1



CC Well to Mod Diff (ODO3866)
0.0
7.2



CC Margin (ODO3866)
16.0
16.6



CC Gr.2 rectosigmoid (ODO3868)
0.0
0.0



CC Margin (ODO3868)
6.3
3.6



CC Mod Diff (ODO3920)
0.9
2.1



CC Margin (ODO3920)
19.1
11.7



CC Gr.2 ascend colon (ODO3921)
8.8
9.2



CC Margin (ODO3921)
6.9
7.5



CC from Partial Hepatectomy
0.9
3.3



(ODO4309) Mets



Liver Margin (ODO4309)
0.7
2.0



Colon mets to lung (OD04451-01)
0.0
0.0



Lung Margin (OD04451-02)
1.9
1.0



Normal Prostate 6546-1
3.9
10.7



Prostate Cancer (OD04410)
17.3
11.6



Prostate Margin (OD04410)
25.0
15.5



Prostate Cancer (OD04720-01)
28.3
11.7



Prostate Margin (OD04720-02)
29.9
20.4



Normal Lung 061010
16.2
15.1



Lung Met to Muscle (ODO4286)
1.2
0.0



Muscle Margin (ODO4286)
8.2
1.6



Lung Malignant Cancer (OD03126)
7.0
2.2



Lung Margin (OD03126)
2.3
3.0



Lung Cancer (OD04404)
8.2
3.1



Lung Margin (OD04404)
11.1
10.2



Lung Cancer (OD04565)
0.0
0.9



Lung Margin (OD04565)
1.9
5.0



Lung Cancer (OD04237-01)
95.3
55.9



Lung Margin (OD04237-02)
4.0
2.3



Ocular Mel Met to Liver (ODO4310)
0.0
0.0



Liver Margin (ODO4310)
2.7
0.8



Melanoma Mets to Lung (OD04321)
0.0
1.7



Lung Margin (OD04321)
10.1
6.6



Normal Kidney
14.8
16.4



Kidney Ca, Nuclear grade 2 (OD04338)
93.3
100.0



Kidney Margin (OD04338)
14.3
2.0



Kidney Ca Nuclear grade 1/2 (OD04339)
5.0
2.0



Kidney Margin (OD04339)
4.1
3.0



Kidney Ca, Clear cell type (OD04340)
27.0
17.4



Kidney Margin (OD04340)
6.4
12.7



Kidney Ca, Nuclear grade 3 (OD04348)
1.9
2.1



Kidney Margin (OD04348)
4.7
6.2



Kidney Cancer (OD04622-01)
50.0
38.4



Kidney Margin (OD04622-03)
4.2
0.0



Kidney Cancer (OD04450-01)
2.6
3.6



Kidney Margin (OD04450-03)
14.2
10.5



Kidney Cancer 8120607
1.9
0.0



Kidney Margin 8120608
1.3
0.9



Kidney Cancer 8120613
1.4
1.6



Kidney Margin 8120614
0.9
1.5



Kidney Cancer 9010320
21.6
16.0



Kidney Margin 9010321
1.9
0.5



Normal Uterus
9.5
10.9



Uterus Cancer 064011
68.3
71.2



Normal Thyroid
3.3
0.8



Thyroid Cancer 064010
2.0
6.9



Thyroid Cancer A302152
8.9
12.2



Thyroid Margin A302153
1.0
0.7



Normal Breast
15.9
15.3



Breast Cancer (OD04566)
4.3
6.3



Breast Cancer (OD04590-01)
7.3
2.8



Breast Cancer Mets (OD04590-03)
9.4
4.8



Breast Cancer Metastasis (OD04655-05)
3.6
0.9



Breast Cancer 064006
8.4
11.6



Breast Cancer 1024
2.6
4.3



Breast Cancer 9100266
1.9
2.0



Breast Margin 9100265
7.4
5.4



Breast Cancer A209073
20.0
13.6



Breast Margin A209073
5.0
12.0



Normal Liver
0.9
1.0



Liver Cancer 064003
0.0
0.0



Liver Cancer 1025
2.4
0.0



Liver Cancer 1026
0.0
0.0



Liver Cancer 6004-T
0.0
1.0



Liver Tissue 6004-N
0.0
0.0



Liver Cancer 6005-T
0.0
0.0



Liver Tissue 6005-N
0.0
0.0



Normal Bladder
18.0
13.8



Bladder Cancer 1023
1.0
0.7



Bladder Cancer A302173
10.9
2.6



Bladder Cancer (OD04718-01)
0.0
1.9



Bladder Normal Adjacent (OD04718-03)
33.2
31.0



Normal Ovary
0.0
4.8



Ovarian Cancer 064008
100.0
62.0



Ovarian Cancer (OD04768-07)
49.7
38.7



Ovary Margin (OD04768-08)
6.3
2.5



Normal Stomach
28.1
18.7



Gastric Cancer 9060358
11.0
14.1



Stomach Margin 9060359
15.9
29.7



Gastric Cancer 9060395
15.0
6.8



Stomach Margin 9060394
26.6
26.4



Gastric Cancer 9060397
2.7
1.7



Stomach Margin 9060396
28.1
33.0



Gastric Cancer 064005
27.7
33.4









Column A - Pel. Exp. (%) Ag2897, Run 161905861






Column B - Rel. Exp. (%) Ag2897, Run 164997312














TABLE AZE










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
0.0



TE671- Medulloblastoma
0.0



D283 Med- Medulloblastoma
0.0



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
0.6



SNB-78- Glioma
6.0



SF-268- Glioblastoma
0.0



T98G- Glioblastoma
0.0



SK-N-SH- Neuroblastoma (metastasis)
0.0



SF-295- Glioblastoma
0.0



Cerebellum
7.1



Cerebellum
0.0



NCI-H292- Mucoepidermoid lung carcinoma
0.0



DMS-114- Small cell lung cancer
0.0



DMS-79- Small cell lung cancer
0.8



NCI-H146- Small cell lung cancer
1.3



NCI-H526- Small cell lung cancer
100.0



NCI-N417- Small cell lung cancer
0.0



NCI-H82- Small cell lung cancer
0.0



NCI-H157- Squamous cell lung cancer (metastasis)
0.0



NCI-H1155- Large cell lung cancer
3.2



NCI-H1299- Large cell lung cancer
1.9



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
2.1



LX-1- Small cell lung cancer
0.0



Colo-205- Colon cancer
0.0



KM12- Colon cancer
2.6



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
31.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
1.5



NCI-SNU-5- Gastric carcinoma
0.0



KATO III- Gastric carcinoma
0.0



NCI-SNU-16- Gastric carcinoma
0.8



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
0.0



RF-48- Gastric adenocarcinoma
0.8



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
0.3



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
0.0



HelaS3- Cervical adenocarcinoma
0.0



Ca Ski- Cervical epidermoid carcinoma (metastasis)
0.0



ES-2- Ovarian clear cell carcinoma
0.8



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
0.0



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
0.0



CA46- Burkitt's lymphoma
0.0



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
0.0



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
0.0



769-P- Clear cell renal carcinoma
0.0



Caki-2- Clear cell renal carcinoma
0.9



SW 839- Clear cell renal carcinoma
0.0



Rhabdoid kidney tumor
0.0



Hs766T- Pancreatic carcinoma (LN metastasis)
0.0



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver metastasis)
0.6



BxPC-3- Pancreatic adenocarcinoma
0.0



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.0



CFPAC-1- Pancreatic ductal adenocarcinoma
0.4



PANC-1- Pancreatic epithelioid ductal carcinoma
0.0



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
0.0



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
0.0



A204- Rhabdomyosarcoma
0.0



HT-1080- Fibrosarcoma
0.8



MG-63- Osteosarcoma
0.0



SK-LMS-1- Leiomyosarcoma (vulva)
0.0



SJRH30- Rhabdomyosarcoma (met to bone marrow)
0.0



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
1.2



DU 145- Prostate carcinoma (brain metastasis)
0.0



MDA-MB-468- Breast adenocarcinoma
0.8



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp. (%) Ag2897, Run 164629841














TABLE AZF










Panel 4D











Tissue Name
A
B















Secondary Th1 act
0.0
0.0



Secondary Th2 act
0.0
0.0



Secondary Tr1 act
0.0
0.0



Secondary Th1 rest
0.0
0.0



Secondary Th2 rest
0.0
0.0



Secondary Tr1 rest
0.0
0.0



Primary Th1 act
0.0
0.0



Primary Th2 act
0.0
0.0



Primary Tr1 act
0.0
0.0



Primary Th1 rest
9.3
20.7



Primary Th2 rest
0.0
9.0



Primary Tr1 rest
9.8
0.0



CD45RA CD4 lymphocyte act
0.0
0.0



CD45RO CD4 lymphocyte act
0.0
0.0



CD8 lymphocyte act
0.0
0.0



Secondary CD8 lymphocyte rest
0.0
0.0



Secondary CD8 lymphocyte act
0.0
0.0



CD4 lymphocyte none
0.0
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0
0.0



LAK cells rest
10.9
0.0



LAK cells IL-2
0.0
0.0



LAK cells IL-2 + IL-12
0.0
0.0



LAK cells IL-2 + IFN gamma
0.0
0.0



LAK cells IL-2 + IL-18
0.0
0.0



LAK cells PMA/ionomycin
0.0
0.0



NK Cells IL-2 rest
0.0
0.0



Two Way MLR 3 day
0.0
9.3



Two Way MLR 5 day
0.0
0.0



Two Way MLR 7 day
0.0
0.0



PBMC rest
0.0
0.0



PBMC PWM
0.0
0.0



PBMC PHA-L
8.9
0.0



Ramos (B cell) none
0.0
0.0



Ramos (B cell) ionomycin
0.0
0.0



B lymphocytes PWM
0.0
0.0



B lymphocytes CD40L and IL-4
0.0
0.0



EOL-1 dbcAMP
0.0
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0
0.0



Dendritic cells none
0.0
0.0



Dendritic cells LPS
0.0
0.0



Dendritic cells anti-CD40
0.0
0.0



Monocytes rest
0.0
0.0



Monocytes LPS
0.0
0.0



Macrophages rest
0.0
0.0



Macrophages LPS
0.0
0.0



HUVEC none
0.0
0.0



HUVEC starved
0.0
0.0



HUVEC IL-1beta
0.0
0.0



HUVEC IFN gamma
0.0
0.0



HUVEC TNF alpha + IFN gamma
0.0
0.0



HUVEC TNF alpha + IL4
0.0
0.0



HUVEC IL-11
0.0
0.0



Lung Microvascular EC none
0.0
0.0



Lung Microvascular EC TNFalpha +
7.9
22.8



IL-1beta



Microvascular Dermal EC none
0.0
0.0



Microsvasular Dermal EC TNFalpha +
16.2
0.0



IL-1beta



Bronchial epithelium TNFalpha + IL1beta
4.4
0.0



Small airway epithelium none
10.4
9.7



Small airway epithelium TNFalpha +
100.0
75.8



IL-1beta



Coronery artery SMC rest
0.0
9.9



Coronery artery SMC TNFalpha + IL-1beta
16.8
9.0



Astrocytes rest
40.6
0.0



Astrocytes TNFalpha + IL-1beta
56.6
100.0



KU-812 (Basophil) rest
0.0
0.0



KU-812 (Basophil) PMA/ionomycin
0.0
0.0



CCD1106 (Keratinocytes) none
0.0
0.0



CCD1106 (Keratinocytes) TNFalpha +
0.0
0.0



IL-1beta



Liver cirrhosis
17.2
8.2



Lupus kidney
8.4
9.6



NCI-H292 none
0.0
0.0



NCI-H292 IL-4
0.0
0.0



NCI-H292 IL-9
0.0
0.0



NCI-H292 IL-13
0.0
0.0



NCI-H292 IFN gamma
0.0
0.0



HPAEC none
0.0
0.0



HPAEC TNF alpha + IL-1 beta
0.0
0.0



Lung fibroblast none
0.0
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0
18.8



Lung fibroblast IL-4
0.0
17.7



Lung fibroblast IL-9
0.0
0.0



Lung fibroblast IL-13
0.0
0.0



Lung fibroblast IFN gamma
0.0
0.0



Dermal fibroblast CCD1070 rest
18.7
5.1



Dermal fibroblast CCD1070 TNF alpha
45.4
29.5



Dermal fibroblast CCD1070 IL-1 beta
33.7
85.9



Dermal fibroblast IFN gamma
0.0
0.0



Dermal fibroblast IL-4
0.0
8.4



IBD Colitis 2
0.0
9.9



IBD Crohn's
7.7
0.0



Colon
62.4
39.2



Lung
9.9
22.1



Thymus
27.7
35.8



Kidney
18.9
20.6









Column A - Rel. Exp. (%) Ag2897, Run 161905862






Column B - Rel. Exp. (%) Ag2897, Run 163586314







AI_comprehensive panel_v1.0 Summary: Ag2897 Highest expression of this gene was detected in a normal tissue sample adjacent to a psoriasis sample (CT=30). This expression was down-regulated in the corresponding psoriasis sample (CT=33). Expression of this gene is useful as a marker to distinguish between these samples. The expression of this gene was up-regulated in lung from emphysema and COPD patients, consistent with its expression in “stressed” small airway epithelium, lung fibroblasts and lung endothelium (treated with TNF-a and IL-1). Therapeutic modulation of the expression of this gene or its protein and/or signaling via this protein by antibodies, small moleculesvor protein therapeutics is useful for the inhibition of inflammation in lung tissue due to asthma, emphysema and other COPD type diseases


Panel 1.3D Summary: Ag2897 Highest expression of this gene, a protocadherin homolog, was detected in the spinal cord and the cerebral cortex (CTs=31.5). Low levels of expression were also seen in other regions of the brain including the amygdala and the hippocampus. The cadherins have been shown to be critical for CNS development, specifically for the guidance of axons, dendrites and/or growth cones in general. Therapeutic modulation of the levels of this protein, or possible signaling via this protein are of utility in enhancing/directing compensatory synaptogenesis and fiber growth in the CNS in response to neuronal death (stroke, head trauma), axon lesion (spinal cord injury), or neurodegeneration (Alzheimer's, Parkinson's, Huntington's, vascular dementia or any neurodegenerative disease). Since protocadherins play an important role in synaptogenesis, this gene product is also involved in depression, schizophrenia, both of which involve synaptogeneisis. Because this cadherin shows highest expression in the cerebellum, this is also an excellent candidate for the spinocerebellar ataxias as well.


Significant levels of expression were also seen in cell lines derived from ovarian, renal and brain cancers. Thus, expression of this gene or gene product is useful as a marker to differentiate between these samples and other samples on this panel and as a marker to detect the presence of these cancers. Therapeutic modulation of the expression or function of this gene or gene product is effective in the treatment of ovarian, renal and brain cancers.


Panel 2D Summary: Ag2897 Highest expression of this gene was detected in ovarian and kidney cancers (CTs=30.5). Significant levels of expression were also seen in lung and uterine cancers. Higher levels of expression were seen in these cancers than in the corresponding normal adjacent tissues. Therapeutic targeting of this gene or gene product with a small molecule drug, protein therapeutic, or human monoclonal antibody is useful for limiting or blocking the extent of tumor cell migration and invasion, preferably in kidney, lung, uterine and ovarian tumor tumors.


Panel 3D Summary: Ag2897 Highest expression of this gene was seen in a lung cancer cell line (CT=30. 1). Expression of this gene or gene product is useful as a marker to differentiate between this sample and other samples on this panel.


Panel 4D Summary: Ag2897 This gene highly was up-regulated in small airway epithelium and astrocytes stimulated with TNF-alpha and IL-1 beta (CTs=33-34). Other tissues in the lung also up regulated the expression of this gene including lung microvascular endothelium and lung fibroblasts in response to TNF alpha or the Th2 elaborated cytokine IL-4. This demonstrates that this molecule is expressed as a result of inflammation, particularly during asthma since TNFalpha and IL-4 may play important roles in the pathology of this disease. Based on the expression profile of this transcript and the types of cytokines which induce it, antibodies, protein therapeutics or small molecule drugs that target this gene or gene product are useful for inhibiting inflammation in lung tissue due to asthma, emphysema and other COPD type diseases.


BA. CG59534-03: Ovostatin


Expression of gene CG59534-03 was assessed using the primer-probe set


Ag7218, described in Table BAA. Results of the RTQ-PCR runs are shown in Table BAB.

TABLE BAAProbe Name Ag7218SEQStartIDPrimersSequencesLengthPositionNoForward5′-ctacaggaagtgcagcacaac-3′212941543ProbeTET-5′-agccagctgaaggcctacctttcctt-3′-263361544TAMRAReverse5′-gttgttcaaactcagatctaacacttct-3′283621545









TABLE BAB










Panel 4.1D










Tissue Name
A














Secondary Th1 act
31.6



Secondary Th2 act
27.4



Secondary Tr1 act
14.4



Secondary Th1 rest
7.4



Secondary Th2 rest
6.6



Secondary Tr1 rest
6.0



Primary Th1 act
9.0



Primary Th2 act
40.1



Primary Tr1 act
39.8



Primary Th1 rest
3.9



Primary Th2 rest
1.6



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
31.4



CD45RO CD4 lymphocyte act
39.8



CD8 lymphocyte act
24.0



Secondary CD8 lymphocyte rest
90.8



Secondary CD8 lymphocyte act
9.5



CD4 lymphocyte none
7.5



2ry Th1/Th2/Tr1_anti-CD95 CH11
11.7



LAK cells rest
20.0



LAK cells IL-2
6.0



LAK cells IL-2 + IL-12
3.6



LAK cells IL-2 + IFN gamma
26.6



LAK cells IL-2 + IL-18
10.4



LAK cells PMA/ionomycin
52.1



NK Cells IL-2 rest
52.9



Two Way MLR 3 day
12.2



Two Way MLR 5 day
9.6



Two Way MLR 7 day
11.9



PBMC rest
2.5



PBMC PWM
21.8



PBMC PHA-L
18.8



Ramos (B cell) none
54.7



Ramos (B cell) ionomycin
79.6



B lymphocytes PWM
13.9



B lymphocytes CD40L and IL-4
16.5



EOL-1 dbcAMP
0.0



EOL-1 dbcAMP PMA/ionomycin
1.3



Dendritic cells none
5.9



Dendritic cells LPS
0.7



Dendritic cells anti-CD40
1.3



Monocytes rest
0.0



Monocytes LPS
5.7



Macrophages rest
1.1



Macrophages LPS
0.0



HUVEC none
17.1



HUVEC starved
45.1



HUVEC IL-1beta
45.7



HUVEC IFN gamma
26.8



HUVEC TNF alpha + IFN gamma
12.0



HUVEC TNF alpha + IL4
26.4



HUVEC IL-11
27.0



Lung Microvascular EC none
28.5



Lung Microvascular EC TNFalpha + IL-1beta
15.5



Microvascular Dermal EC none
8.2



Microsvasular Dermal EC TNFalpha + IL-1beta
6.9



Bronchial epithelium TNFalpha + IL1beta
12.9



Small airway epithelium none
5.1



Small airway epithelium TNFalpha + IL-1beta
10.5



Coronery artery SMC rest
15.8



Coronery artery SMC TNFalpha + IL-1beta
29.3



Astrocytes rest
15.2



Astrocytes TNFalpha + IL-1beta
17.2



KU-812 (Basophil) rest
5.8



KU-812 (Basophil) PMA/ionomycin
11.7



CCD1106 (Keratinocytes) none
15.5



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
8.0



Liver cirrhosis
8.0



NCI-H292 none
2.7



NCI-H292 IL-4
2.0



NCI-H292 IL-9
8.9



NCI-H292 IL-13
2.0



NCI-H292 IFN gamma
5.3



HPAEC none
21.5



HPAEC TNF alpha + IL-1 beta
100.0



Lung fibroblast none
77.9



Lung fibroblast TNF alpha + IL-1 beta
93.3



Lung fibroblast IL-4
33.4



Lung fibroblast IL-9
62.4



Lung fibroblast IL-13
40.9



Lung fibroblast IFN gamma
54.7



Dermal fibroblast CCD1070 rest
8.0



Dermal fibroblast CCD1070 TNF alpha
29.3



Dermal fibroblast CCD1070 IL-1 beta
76.3



Dermal fibroblast IFN gamma
7.0



Dermal fibroblast IL-4
26.1



Dermal Fibroblasts rest
15.8



Neutrophils TNFa + LPS
0.0



Neutrophils rest
2.9



Colon
3.6



Lung
6.3



Thymus
4.7



Kidney
13.7









Column A - Rel. Exp. (%) Ag7218, Run 288211130







Panel 4.1D Summary: Ag7218 Highest expression of this gene was seen in human pulmonary artery endothelial cells (CT=32). Detectable levels of expression were also seen in clusters of lung and dermal fibroblasts as well as in activated T cells. Therefore, targeting this gene or its protein product is useful for the reduction or elimination of the inflammation in lung, skin, and T cell mediated inflammatory and autoimmune diseases.


BB. CG59584-02 and CG59584-03: Ovastatin


Expression of gene CG59584-03 was assessed using the primer-probe set Ag1282, described in Table BBA. Results of the RTQ-PCR runs are shown in Tables BBB, BBC, BBD, BBE, BBF, and BBG.

TABLE BBAProbe Name Ag1282SEQStartIDPrimersSequencesLengthPositionNoForward5′-ttcgcaataaatccagtatggt-3′2245101546ProbeTET-5′-tgctatcaggatttactccaaccatgtca-3′-2945491547TAMRAReverse5′-ttgttttcaagctcttcaatgg-3′2245791548









TABLE BBB










General_screening_panel_v1.4










Tissue Name
A














Adipose
0.5



Melanoma* Hs688(A).T
0.0



Melanoma* Hs688(B).T
0.0



Melanoma* M14
100.0



Melanoma* LOXIMVI
0.9



Melanoma* SK-MEL-5
11.2



Squamous cell carcinoma SCC-4
0.0



Testis Pool
5.3



Prostate ca.* (bone met) PC-3
0.0



Prostate Pool
0.3



Placenta
0.4



Uterus Pool
0.2



Ovarian ca. OVCAR-3
2.1



Ovarian ca. SK-OV-3
0.7



Ovarian ca. OVCAR-4
0.2



Ovarian ca. OVCAR-5
0.8



Ovarian ca. IGROV-1
0.2



Ovarian ca. OVCAR-8
0.1



Ovary
0.6



Breast ca. MCF-7
0.3



Breast ca. MDA-MB-231
0.6



Breast ca. BT 549
0.9



Breast ca. T47D
0.8



Breast ca. MDA-N
45.1



Breast Pool
0.7



Trachea
0.5



Lung
0.3



Fetal Lung
4.9



Lung ca. NCI-N417
0.0



Lung ca. LX-1
1.6



Lung ca. NCI-H146
2.3



Lung ca. SHP-77
1.1



Lung ca. A549
1.1



Lung ca. NCI-H526
0.4



Lung ca. NCI-H23
2.8



Lung ca. NCI-H460
0.1



Lung ca. HOP-62
0.7



Lung ca. NCI-H522
2.0



Liver
0.0



Fetal Liver
0.9



Liver ca. HepG2
0.3



Kidney Pool
1.0



Fetal Kidney
3.6



Renal ca. 786-0
0.6



Renal ca. A498
0.0



Renal ca. ACHN
0.6



Renal ca. UO-31
0.1



Renal ca. TK-10
1.1



Bladder
0.4



Gastric ca. (liver met.) NCI-N87
1.7



Gastric ca. KATO III
1.2



Colon ca. SW-948
0.0



Colon ca. SW480
3.3



Colon ca.* (SW480 met) SW620
1.5



Colon ca. HT29
0.4



Colon ca. HCT-116
2.5



Colon ca. CaCo-2
0.6



Colon cancer tissue
0.5



Colon ca. SW1116
0.0



Colon ca. Colo-205
0.1



Colon ca. SW-48
0.0



Colon Pool
0.5



Small Intestine Pool
1.4



Stomach Pool
0.2



Bone Marrow Pool
0.2



Fetal Heart
1.6



Heart Pool
0.2



Lymph Node Pool
0.4



Fetal Skeletal Muscle
1.6



Skeletal Muscle Pool
0.1



Spleen Pool
0.8



Thymus Pool
2.5



CNS cancer (glio/astro) U87-MG
0.4



CNS cancer (glio/astro) U-118-MG
0.3



CNS cancer (neuro; met) SK-N-AS
0.5



CNS cancer (astro) SF-539
0.1



CNS cancer (astro) SNB-75
9.0



CNS cancer (glio) SNB-19
0.2



CNS cancer (glio) SF-295
0.7



Brain (Amygdala) Pool
0.9



Brain (cerebellum)
0.6



Brain (fetal)
2.9



Brain (Hippocampus) Pool
1.0



Cerebral Cortex Pool
1.1



Brain (Substantia nigra) Pool
0.8



Brain (Thalamus) Pool
1.1



Brain (whole)
0.8



Spinal Cord Pool
0.5



Adrenal Gland
0.1



Pituitary gland Pool
0.1



Salivary Gland
0.1



Thyroid (female)
0.1



Pancreatic ca. CAPAN2
1.3



Pancreas Pool
0.9









Column A - Rel. Exp. (%) Ag1282, Run 216588406














TABLE BBC










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
9.2



Pancreas
0.5



Pancreatic ca. CAPAN 2
1.3



Adrenal gland
0.3



Thyroid
0.3



Salivary gland
0.1



Pituitary gland
0.3



Brain (fetal)
11.1



Brain (whole)
0.7



Brain (amygdala)
1.1



Brain (cerebellum)
0.3



Brain (hippocampus)
1.5



Brain (substantia nigra)
2.3



Brain (thalamus)
2.4



Cerebral Cortex
1.0



Spinal cord
0.7



glio/astro U87-MG
0.4



glio/astro U-118-MG
0.4



astrocytoma SW1783
0.4



neuro*; met SK-N-AS
0.7



astrocytoma SF-539
0.3



astrocytoma SNB-75
7.8



glioma SNB-19
0.2



glioma U251
1.2



glioma SF-295
0.6



Heart (fetal)
5.1



Heart
0.7



Skeletal muscle (fetal)
3.3



Skeletal muscle
0.5



Bone marrow
3.9



Thymus
15.9



Spleen
1.2



Lymph node
4.6



Colorectal
2.5



Stomach
0.6



Small intestine
1.8



Colon ca. SW480
2.8



Colon ca.* SW620(SW480 met)
5.3



Colon ca. HT29
0.3



Colon ca. HCT-116
1.6



Colon ca. CaCo-2
0.8



Colon ca. tissue(ODO3866)
0.2



Colon ca. HCC-2998
3.1



Gastric ca.* (liver met) NCI-N87
1.9



Bladder
0.9



Trachea
0.3



Kidney
0.4



Kidney (fetal)
4.9



Renal ca. 786-0
0.3



Renal ca. A498
1.9



Renal ca. RXF 393
0.1



Renal ca. ACHN
0.8



Renal ca. UO-31
0.4



Renal ca. TK-10
2.1



Liver
0.2



Liver (fetal)
1.4



Liver ca. (hepatoblast) HepG2
0.7



Lung
0.5



Lung (fetal)
8.0



Lung ca. (small cell) LX-1
1.3



Lung ca. (small cell) NCI-H69
13.2



Lung ca. (s. cell var.) SHP-77
5.9



Lung ca. (large cell)NCI-H460
0.1



Lung ca. (non-sm. cell) A549
0.3



Lung ca. (non-s. cell) NCI-H23
2.4



Lung ca. (non-s. cell) HOP-62
1.4



Lung ca. (non-s. cl) NCI-H522
2.6



Lung ca. (squam.) SW 900
2.5



Lung ca. (squam.) NCI-H596
49.7



Mammary gland
2.5



Breast ca.* (pl. ef) MCF-7
0.5



Breast ca.* (pl. ef) MDA-MB-231
0.8



Breast ca.* (pl. ef) T47D
0.3



Breast ca. BT-549
0.1



Breast ca. MDA-N
100.0



Ovary
1.3



Ovarian ca. OVCAR-3
2.6



Ovarian ca. OVCAR-4
0.1



Ovarian ca. OVCAR-5
2.7



Ovarian ca. OVCAR-8
0.1



Ovarian ca. IGROV-1
0.6



Ovarian ca.* (ascites) SK-OV-3
1.3



Uterus
1.4



Placenta
0.2



Prostate
0.5



Prostate ca.* (bone met)PC-3
0.0



Testis
17.4



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
2.8



Melanoma M14
79.6



Melanoma LOX IMVI
2.1



Melanoma* (met) SK-MEL-5
9.9



Adipose
1.9









Column A - Rel. Exp. (%) Ag1282, Run 167614616














TABLE BBD










Panel 2D










Tissue Name
A














Normal Colon
3.3



CC Well to Mod Diff (ODO3866)
0.4



CC Margin (ODO3866)
0.4



CC Gr.2 rectosigmoid (ODO3868)
1.3



CC Margin (ODO3868)
0.1



CC Mod Diff (ODO3920)
3.6



CC Margin (ODO3920)
0.8



CC Gr.2 ascend colon (ODO3921)
0.9



CC Margin (ODO3921)
0.4



CC from Partial Hepatectomy (ODO4309) Mets
0.9



Liver Margin (ODO4309)
0.2



Colon mets to lung (OD04451-01)
0.2



Lung Margin (OD04451-02)
0.2



Normal Prostate 6546-1
1.8



Prostate Cancer (OD04410)
0.8



Prostate Margin (OD04410)
1.1



Prostate Cancer (OD04720-01)
0.7



Prostate Margin (OD04720-02)
1.6



Normal Lung 061010
2.6



Lung Met to Muscle (ODO4286)
0.3



Muscle Margin (ODO4286)
0.2



Lung Malignant Cancer (OD03126)
0.9



Lung Margin (OD03126)
0.7



Lung Cancer (OD04404)
0.5



Lung Margin (OD04404)
0.6



Lung Cancer (OD04565)
0.8



Lung Margin (OD04565)
0.4



Lung Cancer (OD04237-01)
25.5



Lung Margin (OD04237-02)
0.7



Ocular Mel Met to Liver (ODO4310)
100.0



Liver Margin (ODO4310)
0.4



Melanoma Mets to Lung (OD04321)
21.8



Lung Margin (OD04321)
0.5



Normal Kidney
0.5



Kidney Ca, Nuclear grade 2 (OD04338)
0.4



Kidney Margin (OD04338)
0.7



Kidney Ca Nuclear grade 1/2 (OD04339)
0.2



Kidney Margin (OD04339)
0.1



Kidney Ca, Clear cell type (OD04340)
0.8



Kidney Margin (OD04340)
0.6



Kidney Ca, Nuclear grade 3 (OD04348)
0.5



Kidney Margin (OD04348)
0.2



Kidney Cancer (OD04622-01)
0.3



Kidney Margin (OD04622-03)
0.0



Kidney Cancer (OD04450-01)
0.1



Kidney Margin (OD04450-03)
0.1



Kidney Cancer 8120607
0.0



Kidney Margin 8120608
0.0



Kidney Cancer 8120613
0.1



Kidney Margin 8120614
0.1



Kidney Cancer 9010320
0.1



Kidney Margin 9010321
0.1



Normal Uterus
0.3



Uterus Cancer 064011
2.5



Normal Thyroid
0.4



Thyroid Cancer 064010
0.3



Thyroid Cancer A302152
0.2



Thyroid Margin A302153
0.4



Normal Breast
1.8



Breast Cancer (OD04566)
1.6



Breast Cancer (OD04590-01)
0.7



Breast Cancer Mets (OD04590-03)
2.3



Breast Cancer Metastasis (OD04655-05)
0.7



Breast Cancer 064006
0.8



Breast Cancer 1024
1.2



Breast Cancer 9100266
0.9



Breast Margin 9100265
0.5



Breast Cancer A209073
1.6



Breast Margin A209073
1.5



Normal Liver
0.1



Liver Cancer 064003
0.3



Liver Cancer 1025
0.1



Liver Cancer 1026
0.1



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
0.9



Liver Cancer 6005-T
0.3



Liver Tissue 6005-N
0.0



Normal Bladder
1.1



Bladder Cancer 1023
0.2



Bladder Cancer A302173
6.5



Bladder Cancer (OD04718-01)
1.4



Bladder Normal Adjacent (OD04718-03)
0.7



Normal Ovary
0.8



Ovarian Cancer 064008
2.9



Ovarian Cancer (OD04768-07)
3.8



Ovary Margin (OD04768-08)
0.2



Normal Stomach
1.2



Gastric Cancer 9060358
0.8



Stomach Margin 9060359
0.7



Gastric Cancer 9060395
0.8



Stomach Margin 9060394
0.2



Gastric Cancer 9060397
0.5



Stomach Margin 9060396
0.2



Gastric Cancer 064005
2.5









Column A - Rel. Exp. (%) Ag1282, Run 170849610














TABLE BBE










Panel 4.1D










Tissue Name
A














Secondary Th1 act
12.0



Secondary Th2 act
19.3



Secondary Tr1 act
21.6



Secondary Th1 rest
7.5



Secondary Th2 rest
12.1



Secondary Tr1 rest
9.6



Primary Th1 act
20.0



Primary Th2 act
19.6



Primary Tr1 act
24.3



Primary Th1 rest
23.8



Primary Th2 rest
16.3



Primary Tr1 rest
54.0



CD45RA CD4 lymphocyte act
7.6



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
23.3



Secondary CD8 lymphocyte rest
9.3



Secondary CD8 lymphocyte act
16.4



CD4 lymphocyte none
3.6



2ry Th1/Th2/Tr1_anti-CD95 CH11
23.7



LAK cells rest
7.9



LAK cells IL-2
26.8



LAK cells IL-2 + IL-12
5.5



LAK cells IL-2 + IFN gamma
10.8



LAK cells IL-2 + IL-18
14.0



LAK cells PMA/ionomycin
1.5



NK Cells IL-2 rest
29.1



Two Way MLR 3 day
8.0



Two Way MLR 5 day
7.0



Two Way MLR 7 day
9.6



PBMC rest
0.3



PBMC PWM
4.0



PBMC PHA-L
12.7



Ramos (B cell) none
8.2



Ramos (B cell) ionomycin
11.5



B lymphocytes PWM
10.7



B lymphocytes CD40L and IL-4
35.6



EOL-1 dbcAMP
9.2



EOL-1 dbcAMP PMA/ionomycin
10.3



Dendritic cells none
1.2



Dendritic cells LPS
0.7



Dendritic cells anti-CD40
0.3



Monocytes rest
1.1



Monocytes LPS
2.4



Macrophages rest
2.2



Macrophages LPS
0.1



HUVEC none
1.1



HUVEC starved
3.6



HUVEC IL-1beta
2.6



HUVEC IFN gamma
2.6



HUVEC TNF alpha + IFN gamma
1.4



HUVEC TNF alpha + IL4
3.1



HUVEC IL-11
3.4



Lung Microvascular EC none
6.4



Lung Microvascular EC TNFalpha + IL-1beta
9.5



Microvascular Dermal EC none
10.4



Microsvasular Dermal EC TNFalpha + IL-1beta
10.2



Bronchial epithelium TNFalpha + IL1beta
3.4



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
2.7



Coronery artery SMC rest
0.3



Coronery artery SMC TNFalpha + IL-1beta
0.1



Astrocytes rest
1.1



Astrocytes TNFalpha + IL-1beta
2.4



KU-812 (Basophil) rest
14.5



KU-812 (Basophil) PMA/ionomycin
9.4



CCD1106 (Keratinocytes) none
4.6



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
2.4



Liver cirrhosis
2.2



NCI-H292 none
10.6



NCI-H292 IL-4
18.2



NCI-H292 IL-9
24.5



NCI-H292 IL-13
14.8



NCI-H292 IFN gamma
11.1



HPAEC none
3.3



HPAEC TNF alpha + IL-1 beta
5.3



Lung fibroblast none
1.1



Lung fibroblast TNF alpha + IL-1 beta
0.6



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.3



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
5.4



Dermal fibroblast CCD1070 TNF alpha
24.3



Dermal fibroblast CCD1070 IL-1 beta
5.0



Dermal fibroblast IFN gamma
0.3



Dermal fibroblast IL-4
1.6



Dermal Fibroblasts rest
0.8



Neutrophils TNFa + LPS
0.3



Neutrophils rest
0.6



Colon
8.4



Lung
5.1



Thymus
100.0



Kidney
1.3









Column A - Rel. Exp. (%) Ag1282, Run 169828985














TABLE BBF










Panel 5 Islet










Tissue Name
A














97457_Patient-02go_adipose
42.6



97476_Patient-07sk_skeletal muscle
37.9



97477_Patient-07ut_uterus
15.8



97478_Patient-07pl_placenta
85.9



99167_Bayer Patient 1
6.5



97482_Patient-08ut_uterus
13.4



97483_Patient-08pl_placenta
17.4



97486_Patient-09sk_skeletal muscle
8.4



97487_Patient-09ut_uterus
47.6



97488_Patient-09pl_placenta
60.3



97492_Patient-10ut_uterus
27.5



97493_Patient-10pl_placenta
48.3



97495_Patient-11go_adipose
27.5



97496_Patient-11sk_skeletal muscle
2.9



97497_Patient-11ut_uterus
29.7



97498_Patient-11pl_placenta
11.8



97500_Patient-12go_adipose
45.1



97501_Patient-12sk_skeletal muscle
24.7



97502_Patient-12ut_uterus
44.1



97503_Patient-12pl_placenta
8.1



94721_Donor 2 U - A_Mesenchymal Stem Cells
1.2



94722_Donor 2 U - B_Mesenchymal Stem Cells
5.4



94723_Donor 2 U - C_Mesenchymal Stem Cells
1.5



94709_Donor 2 AM - A_adipose
11.9



94710_Donor 2 AM - B_adipose
1.5



94711_Donor 2 AM - C_adipose
1.5



94712_Donor 2 AD - A_adipose
0.0



94713_Donor 2 AD - B_adipose
1.5



94714_Donor 2 AD - C_adipose
11.1



94742_Donor 3 U - A_Mesenchymal Stem Cells
0.0



94743_Donor 3 U - B_Mesenchymal Stem Cells
5.6



94730_Donor 3 AM - A_adipose
0.0



94731_Donor 3 AM - B_adipose
1.7



94732_Donor 3 AM - C_adipose
3.9



94733_Donor 3 AD - A_adipose
1.6



94734_Donor 3 AD - B_adipose
1.8



94735_Donor 3 AD - C_adipose
3.0



77138_Liver_HepG2untreated
55.5



73556_Heart_Cardiac stromal cells (primary)
85.3



81735_Small Intestine
100.0



72409_Kidney_Proximal Convoluted Tubule
23.7



82685_Small intestine_Duodenum
31.4



90650_Adrenal_Adrenocortical adenoma
0.0



72410_Kidney_HRCE
22.8



72411_Kidney_HRE
27.5



73139_Uterus_Uterine smooth muscle cells
3.6









Column A - Rel. Exp. (%) Ag1282, Run 255155568














TABLE BBG










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
7.9



Colon NAT 1
3.5



Colon cancer 2
4.9



Colon NAT 2
2.1



Colon cancer 3
1.5



Colon NAT 3
3.8



Colon malignant cancer 4
18.7



Colon NAT 4
4.0



Lung cancer 1
7.1



Lung NAT 1
2.5



Lung cancer 2
100.0



Lung NAT 2
1.6



Squamous cell carcinoma 3
33.2



Lung NAT 3
1.2



Metastatic melanoma 1
22.8



Melanoma 2
2.0



Melanoma 3
5.7



Metastatic melanoma 4
34.4



Metastatic melanoma 5
62.9



Bladder cancer 1
2.0



Bladder NAT 1
0.0



Bladder cancer 2
6.0



Bladder NAT 2
1.2



Bladder NAT 3
0.5



Bladder NAT 4
8.1



Prostate adenocarcinoma 1
56.6



Prostate adenocarcinoma 2
2.1



Prostate adenocarcinoma 3
4.3



Prostate adenocarcinoma 4
4.1



Prostate NAT 5
2.0



Prostate adenocarcinoma 6
2.2



Prostate adenocarcinoma 7
4.8



Prostate adenocarcinoma 8
0.7



Prostate adenocarcinoma 9
21.8



Prostate NAT 10
0.8



Kidney cancer 1
15.0



Kidney NAT 1
3.1



Kidney cancer 2
49.0



Kidney NAT 2
2.9



Kidney cancer 3
11.0



Kidney NAT 3
1.9



Kidney cancer 4
5.6



Kidney NAT 4
0.2









Column A - Rel. Exp. (%) Ag1282, Run 267211473







General_screening_panel_v1.4 Summary: Ag1282 Highest expression of this gene was seen in a melanoma cell line. In addition, significantly higher levels of expression were seen in a breast cancer cell line. Thus, expression of this is useful as a marker to differentiate between these samples and other samples on this panel and as a marker to detect the presence of melanoma and breast cancer. Furthermore, therapeutic modulation of the expression or function of this gene or gene product is effective in the treatment of melanoma and breast cancers.


Among tissues with metabolic function, this gene was expressed at moderate levels in pituitary, adipose, adrenal gland, pancreas, thyroid, and adult and fetal skeletal muscle, heart, and liver. This widespread expression among these tissues shows that this gene product plays a role in normal neuroendocrine and metabolic function and that disregulated expression of this gene contributes to neuroendocrine disorders or metabolic diseases, such as obesity and diabetes.


In addition, this gene was expressed at much higher levels in fetal lung, liver and skeletal muscle tissue (CTs=27-29) when compared to expression in the adult counterpart (CTs=30-32). Thus, expression of this gene is useful as a marker to differentiate between the fetal and adult source of these tissue.


This molecule is a novel ovostatin that was also expressed at moderate levels in the all regions of the CNS examined. This gene or gene product is a target for the treatment of neurologic diseases.


HASS Panel v1.0 Summary: High levels of expression were detected in samples derived from a glioblastoma and a medulloblastoma.


Panel 1.3D Summary: Ag1282 Expression of this gene was consistent with expression in Panel 1.4. The expression of this gene was highest in a sample derived from a breast cancer cell line (MDA-N) (CT=26.9). In addition, there was expression in other samples derived from lung cancer cell lines and melanoma cell lines. Thus, the expression of this gene is useful as a marker to distinguish MDA-N cells from other samples in the panel. This gene encodes a novel ovostatin. Ovostatins are protease inhibitors that have been shown to support the growth of tumor cells in the absence of serum. They have also been shown to mediate accelerated fibroblast growth, collagen deposition and capillary formation. Thus, therapeutic targeting of this gene product is useful for blocking the uncontrolled growth of cancer cells related to the action of this gene, especially in those cancer types like lung, breast and melanoma tumors where the gene is overexpressed in the tumor compared to the normal adjacent tissue.


Panel 2D Summary: Ag1282 Highest expression of this gene was seen in a sample derived from an ocular melanoma metastasis to the liver (CT=27). In addition, there was expression in other samples derived from lung cancers. Thus, expression of this gene is useful as a marker to distinguish liver cancer cells from other samples in the panel. Moreover, therapeutic modulation of this gene, through the use of small molecule drugs, protein therapeutics or antibodies is of benefit in the treatment of liver or lung cancer.


Panel 4.1D Summary: Ag1282 This gene, an ovostatin-like protein, is related to ovostatin, a known inhibitor of proteinases of all four mechanistic classes, (serine proteinases, cysteine proteinases, aspartyl proteinases, and metalloproteinases) (Saxena I, Cell Mol Life Sci 1997 January;53(1):13-23; Ofuji Y, Periodontal Clin Investig 1992 Fall;14(2):13-22). Highest expression of the gene was seen in the thymus and kidney (CTs=28-29). In addition, moderate to low levels of expression were seen in most of the samples on this panel. This gene or its protein product is useful as a therapeutic protein for the reduction of various proteolytic activities involved in inflammatory and autoimmune diseases such as, but not limited to, Crohn's disease, ulcerative colitis, multiple sclerosis, chronic obstructive pulmonary disease, asthma, emphysema, rheumatoid arthritis, lupus erythematosus, or psoriasis, wound healing, and infection.


Panel 5 Islet Summary: Ag1282 Highest expression of this gene was seen in the small intestine (CT=28), with moderate to low levels of expression seen in metabolic tissues, including adipose, placenta, and skeletal muscle. Thus, targeting this gene or its protein product is useful in the treatment of metabolic disease, including obesity and type II diabetes.


general oncology screening panel_v—2.4 Summary: Ag1282 Highest expression of this gene was seen in a lung cancer sample (CT=28). In addition, this gene was more highly expressed in lung and kidney cancer than in the corresponding normal adjacent tissue. Thus, therapeutic modulation of the expression or function of this gene product is useful in the treatment of lung and kidney cancer.


BC. CG59841-01: Novel Human Agrin


Expression of gene CG59841-01 was assessed using the primer-probe set Ag3605, described in Table BCA. Results of the RTQ-PCR runs are shown in Tables BCB, BCC, BCD and BCE.

TABLE BCAProbe Name Ag3605SEQStartIDPrimersSequencesLengthPositionNoForward5′-gaccccaagtcagaactgttc-3′2131741549ProbeTET-5′-attgagagcaccctggacgacctctt-3′-2632131550TAMRAReverse5′-gaaatccttcttgacgtctgaa-3′2232451551









TABLE BCB










General_screening_panel_v1.4










Tissue Name
A














Adipose
1.4



Melanoma* Hs688(A).T
2.6



Melanoma* Hs688(B).T
4.6



Melanoma* M14
6.7



Melanoma* LOXIMVI
4.8



Melanoma* SK-MEL-5
2.6



Squamous cell carcinoma SCC-4
9.0



Testis Pool
1.3



Prostate ca.* (bone met) PC-3
21.0



Prostate Pool
0.9



Placenta
0.9



Uterus Pool
0.4



Ovarian ca. OVCAR-3
77.4



Ovarian ca. SK-OV-3
42.0



Ovarian ca. OVCAR-4
9.5



Ovarian ca. OVCAR-5
39.2



Ovarian ca. IGROV-1
22.1



Ovarian ca. OVCAR-8
18.0



Ovary
1.5



Breast ca. MCF-7
7.7



Breast ca. MDA-MB-231
22.7



Breast ca. BT 549
13.2



Breast ca. T47D
100.0



Breast ca. MDA-N
4.8



Breast Pool
1.6



Trachea
2.8



Lung
0.2



Fetal Lung
11.4



Lung ca. NCI-N417
1.4



Lung ca. LX-1
10.5



Lung ca. NCI-H146
0.1



Lung ca. SHP-77
1.1



Lung ca. A549
15.6



Lung ca. NCI-H526
5.4



Lung ca. NCI-H23
18.9



Lung ca. NCI-H460
11.5



Lung ca. HOP-62
23.7



Lung ca. NCI-H522
1.8



Liver
0.5



Fetal Liver
0.8



Liver ca. HepG2
15.5



Kidney Pool
1.5



Fetal Kidney
5.8



Renal ca. 786-0
46.3



Renal ca. A498
13.8



Renal ca. ACHN
14.3



Renal ca. UO-31
41.5



Renal ca. TK-10
19.3



Bladder
8.4



Gastric ca. (liver met.) NCI-N87
87.7



Gastric ca. KATO III
17.8



Colon ca. SW-948
9.2



Colon ca. SW480
25.0



Colon ca.* (SW480 met) SW620
5.0



Colon ca. HT29
26.8



Colon ca. HCT-116
4.9



Colon ca. CaCo-2
13.6



Colon cancer tissue
10.2



Colon ca. SW1116
5.1



Colon ca. Colo-205
1.8



Colon ca. SW-48
1.2



Colon Pool
1.9



Small Intestine Pool
0.6



Stomach Pool
1.5



Bone Marrow Pool
0.6



Fetal Heart
1.4



Heart Pool
0.7



Lymph Node Pool
2.1



Fetal Skeletal Muscle
0.9



Skeletal Muscle Pool
0.4



Spleen Pool
0.7



Thymus Pool
1.8



CNS cancer (glio/astro) U87-MG
5.9



CNS cancer (glio/astro) U-118-MG
11.7



CNS cancer (neuro; met) SK-N-AS
1.2



CNS cancer (astro) SF-539
6.7



CNS cancer (astro) SNB-75
22.8



CNS cancer (glio) SNB-19
25.0



CNS cancer (glio) SF-295
35.1



Brain (Amygdala) Pool
1.7



Brain (cerebellum)
1.4



Brain (fetal)
7.0



Brain (Hippocampus) Pool
1.6



Cerebral Cortex Pool
1.9



Brain (Substantia nigra) Pool
2.8



Brain (Thalamus) Pool
2.7



Brain (whole)
3.4



Spinal Cord Pool
1.8



Adrenal Gland
0.2



Pituitary gland Pool
0.3



Salivary Gland
1.1



Thyroid (female)
3.3



Pancreatic ca. CAPAN2
23.7



Pancreas Pool
3.0









Column A - Rel. Exp. (%) Ag3605, Run 213406184














TABLE BCC










Panel 2.2










Tissue Name
A














Normal Colon
4.7



Colon cancer (OD06064)
6.3



Colon Margin (OD06064)
2.3



Colon cancer (OD06159)
2.1



Colon Margin (OD06159)
1.8



Colon cancer (OD06297-04)
2.0



Colon Margin (OD06297-05)
3.0



CC Gr.2 ascend colon (ODO3921)
3.6



CC Margin (ODO3921)
1.3



Colon cancer metastasis (OD06104)
1.1



Lung Margin (OD06104)
1.0



Colon mets to lung (OD04451-01)
4.5



Lung Margin (OD04451-02)
6.7



Normal Prostate
2.1



Prostate Cancer (OD04410)
3.9



Prostate Margin (OD04410)
3.4



Normal Ovary
7.6



Ovarian cancer (OD06283-03)
27.9



Ovarian Margin (OD06283-07)
2.0



Ovarian Cancer 064008
16.0



Ovarian cancer (OD06145)
10.1



Ovarian Margin (OD06145)
8.2



Ovarian cancer (OD06455-03)
28.9



Ovarian Margin (OD06455-07)
1.9



Normal Lung
2.9



Invasive poor diff. lung adeno (ODO4945-01
9.4



Lung Margin (ODO4945-03)
7.9



Lung Malignant Cancer (OD03126)
7.5



Lung Margin (OD03126)
7.0



Lung Cancer (OD05014A)
17.0



Lung Margin (OD05014B)
11.7



Lung cancer (OD06081)
12.2



Lung Margin (OD06081)
2.4



Lung Cancer (OD04237-01)
1.8



Lung Margin (OD04237-02)
16.2



Ocular Melanoma Metastasis
8.4



Ocular Melanoma Margin (Liver)
2.9



Melanoma Metastasis
4.0



Melanoma Margin (Lung)
3.8



Normal Kidney
10.9



Kidney Ca, Nuclear grade 2 (OD04338)
35.4



Kidney Margin (OD04338)
20.4



Kidney Ca Nuclear grade 1/2 (OD04339)
52.9



Kidney Margin (OD04339)
16.6



Kidney Ca, Clear cell type (OD04340)
16.6



Kidney Margin (OD04340)
7.4



Kidney Ca, Nuclear grade 3 (OD04348)
11.2



Kidney Margin (OD04348)
100.0



Kidney malignant cancer (OD06204B)
21.6



Kidney normal adjacent tissue (OD06204E)
23.2



Kidney Cancer (OD04450-01)
53.2



Kidney Margin (OD04450-03)
21.3



Kidney Cancer 8120613
1.1



Kidney Margin 8120614
14.1



Kidney Cancer 9010320
20.3



Kidney Margin 9010321
15.0



Kidney Cancer 8120607
71.7



Kidney Margin 8120608
12.2



Normal Uterus
7.3



Uterine Cancer 064011
6.9



Normal Thyroid
4.3



Thyroid Cancer 064010
27.0



Thyroid Cancer A302152
19.1



Thyroid Margin A302153
8.1



Normal Breast
14.0



Breast Cancer (OD04566)
13.4



Breast Cancer 1024
35.1



Breast Cancer (OD04590-01)
31.6



Breast Cancer Mets (OD04590-03)
8.7



Breast Cancer Metastasis (OD04655-05)
13.3



Breast Cancer 064006
21.5



Breast Cancer 9100266
17.7



Breast Margin 9100265
16.5



Breast Cancer A209073
13.2



Breast Margin A2090734
35.4



Breast cancer (OD06083)
24.5



Breast cancer node metastasis (OD06083)
21.5



Normal Liver
5.0



Liver Cancer 1026
15.5



Liver Cancer 1025
12.2



Liver Cancer 6004-T
7.8



Liver Tissue 6004-N
6.1



Liver Cancer 6005-T
25.0



Liver Tissue 6005-N
12.4



Liver Cancer 064003
12.9



Normal Bladder
14.2



Bladder Cancer 1023
9.5



Bladder Cancer A302173
12.2



Normal Stomach
8.7



Gastric Cancer 9060397
8.9



Stomach Margin 9060396
7.4



Gastric Cancer 9060395
7.0



Stomach Margin 9060394
7.5



Gastric Cancer 064005
6.9









Column A - Rel. Exp. (%) Ag3605, Run 173764229














TABLE BCD










Panel 4.1D










Tissue Name
A














Secondary Th1 act
1.0



Secondary Th2 act
5.1



Secondary Tr1 act
2.5



Secondary Th1 rest
0.0



Secondary Th2 rest
0.4



Secondary Tr1 rest
0.4



Primary Th1 act
3.6



Primary Th2 act
1.1



Primary Tr1 act
3.4



Primary Th1 rest
0.9



Primary Th2 rest
0.5



Primary Tr1 rest
0.2



CD45RA CD4 lymphocyte act
43.5



CD45RO CD4 lymphocyte act
5.0



CD8 lymphocyte act
3.9



Secondary CD8 lymphocyte rest
3.7



Secondary CD8 lymphocyte act
3.3



CD4 lymphocyte none
0.3



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.3



LAK cells rest
5.0



LAK cells IL-2
2.8



LAK cells IL-2 + IL-12
1.4



LAK cells IL-2 + IFN gamma
2.3



LAK cells IL-2 + IL-18
2.9



LAK cells PMA/ionomycin
6.8



NK Cells IL-2 rest
1.6



Two Way MLR 3 day
10.7



Two Way MLR 5 day
6.5



Two Way MLR 7 day
4.3



PBMC rest
0.0



PBMC PWM
5.0



PBMC PHA-L
5.5



Ramos (B cell) none
0.4



Ramos (B cell) ionomycin
0.2



B lymphocytes PWM
2.4



B lymphocytes CD40L and IL-4
1.5



EOL-1 dbcAMP
3.4



EOL-1 dbcAMP PMA/ionomycin
18.3



Dendritic cells none
14.8



Dendritic cells LPS
48.3



Dendritic cells anti-CD40
9.7



Monocytes rest
0.9



Monocytes LPS
66.4



Macrophages rest
16.2



Macrophages LPS
54.7



HUVEC none
9.3



HUVEC starved
14.4



HUVEC IL-1beta
15.6



HUVEC IFN gamma
12.9



HUVEC TNF alpha + IFN gamma
37.6



HUVEC TNF alpha + IL4
31.4



HUVEC IL-11
14.9



Lung Microvascular EC none
79.0



Lung Microvascular EC TNFalpha + IL-1beta
100.0



Microvascular Dermal EC none
49.7



Microsvasular Dermal EC TNFalpha + IL-1beta
56.6



Bronchial epithelium TNFalpha + IL1beta
78.5



Small airway epithelium none
31.0



Small airway epithelium TNFalpha + IL-1beta
81.8



Coronery artery SMC rest
17.2



Coronery artery SMC TNFalpha + IL-1beta
22.2



Astrocytes rest
80.1



Astrocytes TNFalpha + IL-1beta
82.9



KU-812 (Basophil) rest
2.6



KU-812 (Basophil) PMA/ionomycin
0.6



CCD1106 (Keratinocytes) none
70.7



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
77.4



Liver cirrhosis
13.2



NCI-H292 none
57.8



NCI-H292 IL-4
62.4



NCI-H292 IL-9
61.6



NCI-H292 IL-13
53.6



NCI-H292 IFN gamma
67.4



HPAEC none
21.5



HPAEC TNF alpha + IL-1 beta
37.6



Lung fibroblast none
22.4



Lung fibroblast TNF alpha + IL-1 beta
71.2



Lung fibroblast IL-4
16.2



Lung fibroblast IL-9
31.9



Lung fibroblast IL-13
18.7



Lung fibroblast IFN gamma
23.0



Dermal fibroblast CCD1070 rest
15.9



Dermal fibroblast CCD1070 TNF alpha
15.9



Dermal fibroblast CCD1070 IL-1 beta
17.2



Dermal fibroblast IFN gamma
7.2



Dermal fibroblast IL-4
7.2



Dermal Fibroblasts rest
4.3



Neutrophils TNFa + LPS
0.0



Neutrophils rest
0.2



Colon
7.0



Lung
23.3



Thymus
5.8



Kidney
23.2









Column A - Rel. Exp. (%) Ag3605, Run 169943454














TABLE BCE










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
11.6



Colon cancer NAT 1
5.3



Colon cancer 2
18.6



Colon cancer NAT 2
3.2



Colon cancer 3
22.2



Colon cancer NAT 3
7.7



Colon malignant cancer 4
23.5



Colon normal adjacent tissue 4
2.0



Lung cancer 1
57.0



Lung NAT 1
3.1



Lung cancer 2
100.0



Lung NAT 2
3.9



Squamous cell carcinoma 3
41.2



Lung NAT 3
1.7



metastatic melanoma 1
5.4



Melanoma 2
3.2



Melanoma 3
3.7



metastatic melanoma 4
12.5



metastatic melanoma 5
7.9



Bladder cancer 1
0.5



Bladder cancer NAT 1
0.0



Bladder cancer 2
6.0



Bladder cancer NAT 2
0.2



Bladder cancer NAT 3
1.7



Bladder cancer NAT 4
1.8



Prostate adenocarcinoma 1
2.4



Prostate adenocarcinoma 2
2.6



Prostate adenocarcinoma 3
5.2



Prostate adenocarcinoma 4
21.9



Prostate cancer NAT 5
5.4



Prostate adenocarcinoma 6
4.4



Prostate adenocarcinoma 7
3.8



Prostate adenocarcinoma 8
1.8



Prostate adenocarcinoma 9
10.7



Prostate cancer NAT 10
0.7



Kidney cancer 1
38.7



Kidney NAT 1
20.6



Kidney cancer 2
66.4



Kidney NAT 2
39.8



Kidney cancer 3
64.6



Kidney NAT 3
19.6



Kidney cancer 4
16.4



Kidney NAT 4
15.6









Column A - Rel. Exp. (%) Ag3605, Run 260268655







General_screening_panel_v1.4 Summary: Ag3605 Expression of this gene was highest in a breast cancer cell line (CT=25.2). Expression of this gene was primarily associated with cancer cell lines rather than with normal tissues and was upregulated in pancreatic, CNS, colon, gastric, renal, lung, breast, ovarian, and prostate cancer cell lines when compared to their respective normal tissues. Thus, therapeutic modulation of the activity of this gene or its protein product, using small molecule drugs, antibodies or protein therapeutics, is of benefit in the treatment of these types of cancers.


In addition, this gene was expressed at moderate levels in all regions of the central nervous system examined, including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. This gene encodes a protein with homology to agrin, a neuronal aggregating factor that induces the aggregation of acetylcholine receptors and other postsynaptic proteins on muscle fibers and is crucial for the formation of the neuromuscular junction. Arin plays an important role in defining neuronal responses to excitatory neurotransmitters both in vitro and in vivo (Hilgenberg LG, Mol Cell Neurosci 2002 January;19(1):97-110; Bixby J L,. J Neurobiol 2002 February 5;50(2):164-79). This gene expression in the central nervous system was consistent with the hypothesis that this protein functions as agrin. Therefore, this gene plays a role in central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Among tissues with metabolic or endocrine function, this gene was expressed at moderate levels in pancreas, adipose, thyroid, and the gastrointestinal tract and at low levels in adrenal gland, pituitary gland, skeletal muscle, heart, and liver. Therefore, therapeutic modulation of the activity of this gene is useful in the treatment of endocrine/metabolically related diseases, such as obesity and diabetes.


Panel 2.2 Summary: Ag3605 Expression of this gene was highest in a sample of normal kidney (CT=27.4). In addition, expression of this gene was upregulated in a number of ovarian and renal cancers when compared to the matched control margins. Expression of this gene is useful as a marker for ovarian and renal carcinoma. Therapeutic modulation of the activity of this gene or its protein product, using small molecule drugs, antibodies or protein therapeutics, is of benefit in the treatment of renal and ovarian cancer.


Panel 4.1D Summary: Ag3605 Expression of this gene was highest in lung microvascular endothelial cells, microvascular dermal endothelial cells, mucoepidermoid cell line NCI-H292, astrocytes, and keratinocytes. Therefore, small molecule drug, antibody or protein therapeutics designed against the protein encoded by this gene is useful for the reduction or inhibition of inflammation in asthma, emphysema, allergy, psoriasis, muscular dystrophy and multiple sclerosis.


general oncology screening panel_V—2.4 Summary: Ag3605 Highest expression of this gene was seen in lung cancer (CT=26.8). Higher levels of expression were seen in lung and kidney cancers when compared to expression in normal adjacent tissue. Expression of this gene is useful as a marker of lung and kidney cancers. Therapeutic modulation of the expression or function of this gene product is useful in the treatment of lung and kidney cancers.


BD. CG59905-01 and CG59905-02: Sushi Containing Membrane Protein


Expression of gene CG59905-01 and variant CG59905-02 was assessed using the primer-probe set Ag2443, described in Table BDA. Results of the RTQ-PCR runs are

TABLE BDAProbe Name Ag2443SEQStartIDPrimersSequencesLengthPositionNoForward5′-cagtttgggaccgacttca-3′196521552ProbeTET-5′-caagactgtgagctatcagtgtaaccca-3′-286781553TAMRAReverse5′-tgactgcttccatgacatagc-3′217071554









TABLE BDB










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
16.6



Pancreas 1
0.0



Pancreatic ca. CAPAN 2
0.0



Adrenal gland
1.5



Thyroid
0.0



Salivary gland
0.0



Pituitary gland
2.2



Brain (fetal)
51.4



Brain (whole)
92.0



Brain (amygdala)
52.1



Brain (cerebellum)
47.6



Brain (hippocampus)
78.5



Brain (substantia nigra)
8.0



Brain (thalamus)
12.9



Cerebral Cortex
100.0



Spinal cord
6.5



glio/astro U87-MG
0.0



glio/astro U-118-MG
0.0



astrocytoma SW1783
0.0



neuro*; met SK-N-AS
0.0



astrocytoma SF-539
0.0



astrocytoma SNB-75
0.0



glioma SNB-19
0.6



glioma U251
29.9



glioma SF-295
1.9



Heart (fetal)
0.0



Heart
0.0



Skeletal muscle (fetal)
6.2



Skeletal muscle
0.8



Bone marrow
0.0



Thymus
0.0



Spleen
5.4



Lymph node
0.5



Colorectal
0.7



Stomach
0.0



Small intestine
1.5



Colon ca. SW480
0.0



Colon ca.* SW620(SW480 met)
0.0



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
0.0



Colon ca. HCC-2998
0.0



Gastric ca.* (liver met) NCI-N87
0.0



Bladder
1.2



Trachea
2.0



Kidney
4.1



Kidney (fetal)
2.5



Renal ca. 786-0
0.0



Renal ca. A498
0.0



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.0



Renal ca. TK-10
0.0



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
0.0



Lung (fetal)
1.4



Lung ca. (small cell) LX-1
0.0



Lung ca. (small cell) NCI-H69
0.0



Lung ca. (s. cell var.) SHP-77
16.8



Lung ca. (large cell)NCI-H460
1.6



Lung ca. (non-sm. cell) A549
0.0



Lung ca. (non-s. cell) NCI-H23
11.2



Lung ca. (non-s. cell) HOP-62
0.0



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
0.0



Lung ca. (squam.) NCI-H596
4.8



Mammary gland
2.1



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
10.9



Breast ca. BT-549
0.0



Breast ca. MDA-N
9.3



Ovary
1.1



Ovarian ca. OVCAR-3
11.5



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
0.0



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
0.8



Uterus
2.8



Placenta
2.9



Prostate
0.9



Prostate ca.* (bone met)PC-3
0.0



Testis
20.9



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.6



Melanoma UACC-62
19.5



Melanoma M14
15.1



Melanoma LOX IMVI
0.7



Melanoma* (met) SK-MEL-5
7.3



Adipose
0.4









Column A - Rel. Exp. (%) Ag2443, Run 165517738














TABLE BDC










Panel 2D










Tissue Name
A














Normal Colon
12.6



CC Well to Mod Diff (ODO3866)
1.2



CC Margin (ODO3866)
2.9



CC Gr.2 rectosigmoid (ODO3868)
1.9



CC Margin (ODO3868)
3.2



CC Mod Diff (ODO3920)
1.5



CC Margin (ODO3920)
2.6



CC Gr.2 ascend colon (ODO3921)
9.3



CC Margin (ODO3921)
2.9



CC from Partial Hepatectomy (ODO4309) Mets
0.0



Liver Margin (ODO4309)
0.0



Colon mets to lung (OD04451-01)
2.5



Lung Margin (OD04451-02)
18.0



Normal Prostate 6546-1
4.2



Prostate Cancer (OD04410)
0.0



Prostate Margin (OD04410)
5.3



Prostate Cancer (OD04720-01)
6.0



Prostate Margin (OD04720-02)
4.5



Normal Lung 061010
29.9



Lung Met to Muscle (ODO4286)
2.4



Muscle Margin (ODO4286)
3.1



Lung Malignant Cancer (OD03126)
12.2



Lung Margin (OD03126)
20.4



Lung Cancer (OD04404)
3.0



Lung Margin (OD04404)
30.1



Lung Cancer (OD04565)
0.8



Lung Margin (OD04565)
9.0



Lung Cancer (OD04237-01)
7.8



Lung Margin (OD04237-02)
3.1



Ocular Mel Met to Liver (ODO4310)
3.2



Liver Margin (ODO4310)
1.1



Melanoma Mets to Lung (OD04321)
0.0



Lung Margin (OD04321)
17.2



Normal Kidney
100.0



Kidney Ca, Nuclear grade 2 (OD04338)
0.0



Kidney Margin (OD04338)
16.8



Kidney Ca Nuclear grade 1/2 (OD04339)
6.3



Kidney Margin (OD04339)
59.0



Kidney Ca, Clear cell type (OD04340)
10.7



Kidney Margin (OD04340)
38.4



Kidney Ca, Nuclear grade 3 (OD04348)
0.8



Kidney Margin (OD04348)
18.4



Kidney Cancer (OD04622-01)
1.8



Kidney Margin (OD04622-03)
8.9



Kidney Cancer (OD04450-01)
1.6



Kidney Margin (OD04450-03)
21.3



Kidney Cancer 8120607
0.0



Kidney Margin 8120608
37.1



Kidney Cancer 8120613
0.0



Kidney Margin 8120614
31.6



Kidney Cancer 9010320
4.2



Kidney Margin 9010321
62.9



Normal Uterus
1.4



Uterus Cancer 064011
19.6



Normal Thyroid
0.6



Thyroid Cancer 064010
6.6



Thyroid Cancer A302152
0.0



Thyroid Margin A302153
1.9



Normal Breast
22.2



Breast Cancer (OD04566)
0.8



Breast Cancer (OD04590-01)
2.6



Breast Cancer Mets (OD04590-03)
2.0



Breast Cancer Metastasis (OD04655-05)
2.9



Breast Cancer 064006
4.3



Breast Cancer 1024
6.5



Breast Cancer 9100266
6.5



Breast Margin 9100265
15.7



Breast Cancer A209073
10.7



Breast Margin A209073
31.4



Normal Liver
0.0



Liver Cancer 064003
26.6



Liver Cancer 1025
0.0



Liver Cancer 1026
0.0



Liver Cancer 6004-T
0.0



Liver Tissue 6004-N
29.1



Liver Cancer 6005-T
0.0



Liver Tissue 6005-N
0.0



Normal Bladder
0.4



Bladder Cancer 1023
0.0



Bladder Cancer A302173
39.0



Bladder Cancer (OD04718-01)
0.0



Bladder Normal Adjacent (OD04718-03)
4.5



Normal Ovary
2.6



Ovarian Cancer 064008
0.0



Ovarian Cancer (OD04768-07)
0.0



Ovary Margin (OD04768-08)
0.0



Normal Stomach
2.2



Gastric Cancer 9060358
0.0



Stomach Margin 9060359
1.3



Gastric Cancer 9060395
4.6



Stomach Margin 9060394
5.3



Gastric Cancer 9060397
2.5



Stomach Margin 9060396
0.8



Gastric Cancer 064005
0.0









Column A - Rel. Exp. (%) Ag2443, Run 164988396














TABLE BDD










Panel 4D










Tissue Name
A














Secondary Th1 act
0.0



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
0.0



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.0



LAK cells rest
0.0



LAK cells IL-2
0.0



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
0.0



LAK cells IL-2 + IL-18
0.0



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
0.0



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
0.1



PBMC PWM
0.0



PBMC PHA-L
0.0



Ramos (B cell) none
35.6



Ramos (B cell) ionomycin
100.0



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
0.5



EOL-1 dbcAMP PMA/ionomycin
0.0



Dendritic cells none
0.2



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
0.1



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
0.0



HUVEC IL-1beta
0.0



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
0.0



Lung Microvascular EC none
0.0



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
0.0



Small airway epithelium none
0.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
0.0



Astrocytes TNFalpha + IL-1beta
0.0



KU-812 (Basophil) rest
0.7



KU-812 (Basophil) PMA/ionomycin
0.4



CCD1106 (Keratinocytes) none
0.0



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
0.0



Liver cirrhosis
0.2



Lupus kidney
0.1



NCI-H292 none
0.0



NCI-H292 IL-4
0.0



NCI-H292 IL-9
0.0



NCI-H292 IL-13
0.0



NCI-H292 IFN gamma
0.0



HPAEC none
0.2



HPAEC TNF alpha + IL-1 beta
0.1



Lung fibroblast none
0.0



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
0.0



Lung fibroblast IL-9
0.0



Lung fibroblast IL-13
0.0



Lung fibroblast IFN gamma
0.0



Dermal fibroblast CCD1070 rest
0.0



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



IBD Colitis 2
0.0



IBD Crohn's
0.0



Colon
0.1



Lung
0.1



Thymus
2.5



Kidney
0.1









Column A - Rel. Exp. (%) Ag2443, Run 164337611







Panel 1.3D Summary: Ag2443 Highest expression of this gene was detected in the cerebral cortex (CT=30.9). In addition, high expression of this gene was observed exclusively in all the brain regions (CTs=31). Expression of this gene is useful as a marker to distinguish these brain samples from other samples used in this panel. Low but significant expression was also seen in spinal cord region. Therefore, therapeutic modulation of the function or activity of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies, is beneficial in the treatment of central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


This gene encodes a variant of cub and sushi multiple domains 1 protein (CSMD1). A related protein, SEZ-6, has recently been shown to be associated with neuronal bursting activity of seizures (Shimizu-Nishikawa K, Brain Res Mol Brain Res 28(2):201-10). Thus, the protein encoded by this gene also plays a role in neuronal siezures.


In addition, low to moderate expression of this gene was seen in melanoma, ovarian cancer OVCAR-3, breast cancer, lung cancer, liver adenocarcinoma and glioma U25 1 cell lines. Therapeutic modulation of the activity of this gene or its protein product, through the use of small molecule drugs, or antibodies, is beneficial in the treatment of these cancers.


Significant expression was also detected in fetal skeletal muscle.


Panel 2D Summary: Ag2443 Highest expression of this gene was detected in a kidney sample (CT=31). Expression of this gene was down-regulated in kidney and lung cancer. In addition, there was substantial expression in other samples derived from bladder cancer, liver cancer, uterine cancer, breast cancer, kidney cancer and lung cancer. This gene encodes a variant of cub and sushi multiple domains 1 protein (CSMD1). Recently, CSMD1 has been shown to be a candidate for tumor suppressor of multiple types of cancer (Sun PC. Genomics 75(1-3):17-25). Therefore, therapeutic modulation of the activity of this gene or its protein product, through the use of small molecule drugs, or antibodies, is beneficial in the treatment of bladder, liver, uterine, breast, kidney and lung cancers.


Panel 4D Summary: Ag2443 Highest expression of this gene was seen in Ramos B cells (CTs=26-27). Expression of this gene is useful as a marker to distinguish these samples from other samples in this panel. In addition, expression of this gene in B cells shows that this gene is involved in rheumatic disease including rheumatoid arthritis, lupus, osteoarthritis, and hyperproliferative B cell disorders.


Low but significant expression of this gene was also detected in thymus. Drugs that inhibit the function of this protein are useful in the regulation of T cell development in the thymus and for the reduction or elimination of the symptoms of T cell mediated autoimmune or inflammatory diseases, including asthma, allergies, inflammatory bowel disease, lupus erythematosus, or rheumatoid arthritis. Small molecule or antibody therapeutics designed against this putative protein are useful for the disruption of T cell development in the thymus and as immunosuppresants for tissue transplant.


BE. CG9271501: KIAA0918


Expression of gene CG92715-01 was assessed using the primer-probe set Ag2502, described in Table BEA. Results of the RTQ-PCR runs are shown in Tables BEB, BEC, BED and BEE.

TABLE BEAProbe Name Ag2502SEQStartIDPrimersSequencesLengthPositionNoForward5′-ggagtaaccacttcacctcctt-3′2216321555ProbeTET-5′-ccagctgaagtcactcatccaaatcg-3′-2616751556TAMRAReverse5′-aggtacaatcccaaggattgtc-3′2217091557









TABLE BEB










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
4.5



Pancreas
1.1



Pancreatic ca. CAPAN 2
14.9



Adrenal gland
0.8



Thyroid
10.1



Salivary gland
17.3



Pituitary gland
2.9



Brain (fetal)
76.3



Brain (whole)
95.3



Brain (amygdala)
80.1



Brain (cerebellum)
61.1



Brain (hippocampus)
100.0



Brain (substantia nigra)
16.2



Brain (thalamus)
25.0



Cerebral Cortex
92.0



Spinal cord
20.0



glio/astro U87-MG
24.7



glio/astro U-118-MG
22.4



astrocytoma SW1783
0.2



neuro*; met SK-N-AS
42.3



astrocytoma SF-539
0.0



astrocytoma SNB-75
3.6



glioma SNB-19
2.5



glioma U251
19.5



glioma SF-295
0.0



Heart (fetal)
1.3



Heart
0.8



Skeletal muscle (fetal)
8.2



Skeletal muscle
0.0



Bone marrow
0.0



Thymus
0.7



Spleen
0.5



Lymph node
0.3



Colorectal
0.7



Stomach
3.1



Small intestine
2.4



Colon ca. SW480
0.2



Colon ca.* SW620(SW480 met)
1.4



Colon ca. HT29
0.0



Colon ca. HCT-116
0.0



Colon ca. CaCo-2
0.0



Colon ca. tissue(ODO3866)
0.0



Colon ca. HCC-2998
4.8



Gastric ca.* (liver met) NCI-N87
0.2



Bladder
2.6



Trachea
4.1



Kidney
0.6



Kidney (fetal)
1.3



Renal ca. 786-0
31.2



Renal ca. A498
4.4



Renal ca. RXF 393
0.0



Renal ca. ACHN
1.7



Renal ca. UO-31
4.2



Renal ca. TK-10
5.8



Liver
0.0



Liver (fetal)
0.0



Liver ca. (hepatoblast) HepG2
0.0



Lung
0.3



Lung (fetal)
2.1



Lung ca. (small cell) LX-1
2.1



Lung ca. (small cell) NCI-H69
4.8



Lung ca. (s. cell var.) SHP-77
30.6



Lung ca. (large cell)NCI-H460
21.2



Lung ca. (non-sm. cell) A549
2.3



Lung ca. (non-s. cell) NCI-H23
36.1



Lung ca. (non-s. cell) HOP-62
10.8



Lung ca. (non-s. cl) NCI-H522
0.3



Lung ca. (squam.) SW 900
3.2



Lung ca. (squam.) NCI-H596
23.2



Mammary gland
1.5



Breast ca.* (pl. ef) MCF-7
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0



Breast ca.* (pl. ef) T47D
4.9



Breast ca. BT-549
14.2



Breast ca. MDA-N
1.4



Ovary
3.6



Ovarian ca. OVCAR-3
12.9



Ovarian ca. OVCAR-4
4.1



Ovarian ca. OVCAR-5
0.0



Ovarian ca. OVCAR-8
11.7



Ovarian ca. IGROV-1
0.0



Ovarian ca.* (ascites) SK-OV-3
1.6



Uterus
0.0



Placenta
0.0



Prostate
1.2



Prostate ca.* (bone met)PC-3
0.6



Testis
0.8



Melanoma Hs688(A).T
0.0



Melanoma* (met) Hs688(B).T
0.0



Melanoma UACC-62
1.1



Melanoma M14
2.7



Melanoma LOX IMVI
0.8



Melanoma* (met) SK-MEL-5
2.3



Adipose
1.3









Column A - Rel. Exp. (%) Ag2502, Run 165518160














TABLE BEC










Panel 2D











Tissue Name
A
B















Normal Colon
7.1
9.9



CC Well to Mod Diff (ODO3866)
0.0
0.1



CC Margin (ODO3866)
0.7
0.3



CC Gr.2 rectosigmoid (ODO3868)
0.3
0.0



CC Margin (ODO3868)
0.4
0.8



CC Mod Diff (ODO3920)
10.5
11.1



CC Margin (ODO3920)
1.0
1.5



CC Gr.2 ascend colon (ODO3921)
2.8
1.3



CC Margin (ODO3921)
0.1
0.2



CC from Partial Hepatectomy
0.0
0.0



(ODO4309) Mets



Liver Margin (ODO4309)
0.1
0.1



Colon mets to lung (OD04451-01)
0.9
1.3



Lung Margin (OD04451-02)
0.9
0.4



Normal Prostate 6546-1
13.8
7.2



Prostate Cancer (OD04410)
19.1
15.9



Prostate Margin (OD04410)
7.0
8.5



Prostate Cancer (OD04720-01)
33.2
34.9



Prostate Margin (OD04720-02)
43.5
56.6



Normal Lung 061010
2.8
3.0



Lung Met to Muscle (ODO4286)
15.8
14.3



Muscle Margin (ODO4286)
0.5
0.6



Lung Malignant Cancer (OD03126)
0.9
0.7



Lung Margin (OD03126)
1.2
1.9



Lung Cancer (OD04404)
0.3
0.3



Lung Margin (OD04404)
0.4
0.5



Lung Cancer (OD04565)
0.1
0.5



Lung Margin (OD04565)
0.3
1.0



Lung Cancer (OD04237-01)
1.7
1.7



Lung Margin (OD04237-02)
0.1
0.0



Ocular Mel Met to Liver (ODO4310)
0.0
0.0



Liver Margin (ODO4310)
0.2
0.0



Melanoma Mets to Lung (OD04321)
0.0
0.1



Lung Margin (OD04321)
0.4
0.6



Normal Kidney
2.3
1.9



Kidney Ca, Nuclear grade 2 (OD04338)
2.8
2.9



Kidney Margin (OD04338)
0.4
0.3



Kidney Ca Nuclear grade 1/2 (OD04339)
0.4
0.3



Kidney Margin (OD04339)
0.4
0.9



Kidney Ca, Clear cell type (OD04340)
27.4
33.2



Kidney Margin (OD04340)
0.2
0.3



Kidney Ca, Nuclear grade 3 (OD04348)
3.0
3.0



Kidney Margin (OD04348)
0.4
0.0



Kidney Cancer (OD04622-01)
100.0
100.0



Kidney Margin (OD04622-03)
0.0
0.5



Kidney Cancer (OD04450-01)
0.1
0.8



Kidney Margin (OD04450-03)
0.8
0.7



Kidney Cancer 8120607
4.7
3.7



Kidney Margin 8120608
0.1
0.1



Kidney Cancer 8120613
0.0
0.1



Kidney Margin 8120614
0.2
0.6



Kidney Cancer 9010320
1.4
2.1



Kidney Margin 9010321
0.6
0.2



Normal Uterus
0.1
0.1



Uterus Cancer 064011
0.3
0.7



Normal Thyroid
14.5
13.5



Thyroid Cancer 064010
0.4
0.3



Thyroid Cancer A302152
0.2
0.6



Thyroid Margin A302153
14.9
12.4



Normal Breast
1.7
2.6



Breast Cancer (OD04566)
0.1
0.2



Breast Cancer (OD04590-01)
5.5
5.1



Breast Cancer Mets (OD04590-03)
4.2
2.6



Breast Cancer Metastasis (OD04655-05)
1.0
0.9



Breast Cancer 064006
1.3
0.4



Breast Cancer 1024
2.1
1.1



Breast Cancer 9100266
0.1
0.1



Breast Margin 9100265
2.5
2.5



Breast Cancer A209073
2.4
0.6



Breast Margin A209073
3.7
3.3



Normal Liver
0.0
0.1



Liver Cancer 064003
0.6
1.2



Liver Cancer 1025
0.0
0.0



Liver Cancer 1026
0.0
0.1



Liver Cancer 6004-T
0.0
0.0



Liver Tissue 6004-N
0.3
0.2



Liver Cancer 6005-T
0.1
0.0



Liver Tissue 6005-N
0.0
0.0



Normal Bladder
6.5
4.0



Bladder Cancer 1023
0.0
0.3



Bladder Cancer A302173
24.0
23.5



Bladder Cancer (OD04718-01)
0.0
0.0



Bladder Normal Adjacent (OD04718-03)
0.1
0.1



Normal Ovary
2.1
3.9



Ovarian Cancer 064008
4.9
6.3



Ovarian Cancer (OD04768-07)
10.4
7.8



Ovary Margin (OD04768-08)
0.4
0.2



Normal Stomach
6.2
5.2



Gastric Cancer 9060358
3.1
7.5



Stomach Margin 9060359
1.3
0.8



Gastric Cancer 9060395
4.7
4.3



Stomach Margin 9060394
1.5
1.3



Gastric Cancer 9060397
1.4
1.2



Stomach Margin 9060396
0.2
0.2



Gastric Cancer 064005
4.7
3.0









Column A - Rel. Exp. (%) Ag2502, Run 162319639






Column B - Rel. Exp. (%) Ag2502, Run 164993363














TABLE BED










Panel 3D










Tissue Name
A














Daoy- Medulloblastoma
1.6



TE671- Medulloblastoma
41.8



D283 Med- Medulloblastoma
63.7



PFSK-1- Primitive Neuroectodermal
0.0



XF-498- CNS
22.8



SNB-78- Glioma
11.8



SF-268- Glioblastoma
5.1



T98G- Glioblastoma
0.0



SK-N-SH- Neuroblastoma (metastasis)
0.0



SF-295- Glioblastoma
0.8



Cerebellum
79.0



Cerebellum
26.4



NCI-H292- Mucoepidermoid lung carcinoma
1.5



DMS-114- Small cell lung cancer
13.2



DMS-79- Small cell lung cancer
0.0



NCI-H146- Small cell lung cancer
28.5



NCI-H526- Small cell lung cancer
3.1



NCI-N417- Small cell lung cancer
46.3



NCI-H82- Small cell lung cancer
87.1



NCI-H157- Squamous cell lung cancer (metastasis)
100.0



NCI-H1155- Large cell lung cancer
35.6



NCI-H1299- Large cell lung cancer
16.5



NCI-H727- Lung carcinoid
0.0



NCI-UMC-11- Lung carcinoid
53.2



LX-1- Small cell lung cancer
1.2



Colo-205- Colon cancer
5.9



KM12- Colon cancer
0.0



KM20L2- Colon cancer
0.0



NCI-H716- Colon cancer
0.0



SW-48- Colon adenocarcinoma
0.0



SW1116- Colon adenocarcinoma
0.0



LS 174T- Colon adenocarcinoma
0.0



SW-948- Colon adenocarcinoma
0.0



SW-480- Colon adenocarcinoma
0.0



NCI-SNU-5- Gastric carcinoma
0.0



KATO III- Gastric carcinoma
0.0



NCI-SNU-16- Gastric carcinoma
6.7



NCI-SNU-1- Gastric carcinoma
0.0



RF-1- Gastric adenocarcinoma
2.4



RF-48- Gastric adenocarcinoma
2.6



MKN-45- Gastric carcinoma
0.0



NCI-N87- Gastric carcinoma
0.0



OVCAR-5- Ovarian carcinoma
0.0



RL95-2- Uterine carcinoma
1.7



HelaS3- Cervical adenocarcinoma
14.6



Ca Ski- Cervical epidermoid carcinoma (metastasis)
1.2



ES-2- Ovarian clear cell carcinoma
22.5



Ramos- Stimulated with PMA/ionomycin 6 h
0.0



Ramos- Stimulated with PMA/ionomycin 14 h
0.0



MEG-01- Chronic myelogenous leukemia
13.8



(megokaryoblast)



Raji- Burkitt's lymphoma
0.0



Daudi- Burkitt's lymphoma
0.0



U266- B-cell plasmacytoma
8.5



CA46- Burkitt's lymphoma
6.3



RL- non-Hodgkin's B-cell lymphoma
0.0



JM1- pre-B-cell lymphoma
0.0



Jurkat- T cell leukemia
0.0



TF-1- Erythroleukemia
11.8



HUT 78- T-cell lymphoma
0.0



U937- Histiocytic lymphoma
0.0



KU-812- Myelogenous leukemia
0.3



769-P- Clear cell renal carcinoma
9.5



Caki-2- Clear cell renal carcinoma
3.1



SW 839- Clear cell renal carcinoma
12.5



Rhabdoid kidney tumor
12.6



Hs766T- Pancreatic carcinoma (LN metastasis)
0.0



CAPAN-1- Pancreatic adenocarcinoma (liver
0.0



metastasis)



SU86.86- Pancreatic carcinoma (liver metastasis)
2.4



BxPC-3- Pancreatic adenocarcinoma
0.0



HPAC- Pancreatic adenocarcinoma
0.0



MIA PaCa-2- Pancreatic carcinoma
0.7



CFPAC-1- Pancreatic ductal adenocarcinoma
0.4



PANC-1- Pancreatic epithelioid ductal carcinoma
1.3



T24- Bladder carcinma (transitional cell)
0.0



5637- Bladder carcinoma
7.7



HT-1197- Bladder carcinoma
0.0



UM-UC-3- Bladder carcinma (transitional cell)
3.1



A204- Rhabdomyosarcoma
0.0



HT-1080- Fibrosarcoma
2.6



MG-63- Osteosarcoma
4.6



SK-LMS-1- Leiomyosarcoma (vulva)
0.3



SJRH30- Rhabdomyosarcoma (met to bone marrow)
22.8



A431- Epidermoid carcinoma
0.0



WM266-4- Melanoma
15.8



DU 145- Prostate carcinoma (brain metastasis)
0.0



MDA-MB-468- Breast adenocarcinoma
0.3



SCC-4- Squamous cell carcinoma of tongue
0.0



SCC-9- Squamous cell carcinoma of tongue
0.0



SCC-15- Squamous cell carcinoma of tongue
0.0



CAL 27- Squamous cell carcinoma of tongue
0.0









Column A - Rel. Exp. (%) Ag2502, Run 164629448














TABLE BEE










Panel 4D










Tissue Name
A














Secondary Th1 act
1.6



Secondary Th2 act
0.0



Secondary Tr1 act
0.0



Secondary Th1 rest
0.0



Secondary Th2 rest
0.0



Secondary Tr1 rest
0.0



Primary Th1 act
0.0



Primary Th2 act
0.0



Primary Tr1 act
0.0



Primary Th1 rest
0.0



Primary Th2 rest
0.0



Primary Tr1 rest
1.8



CD45RA CD4 lymphocyte act
0.0



CD45RO CD4 lymphocyte act
0.0



CD8 lymphocyte act
0.0



Secondary CD8 lymphocyte rest
0.0



Secondary CD8 lymphocyte act
0.0



CD4 lymphocyte none
3.1



2ry Th1/Th2/Tr1_anti-CD95 CH11
1.1



LAK cells rest
4.1



LAK cells IL-2
6.6



LAK cells IL-2 + IL-12
0.0



LAK cells IL-2 + IFN gamma
2.4



LAK cells IL-2 + IL-18
4.4



LAK cells PMA/ionomycin
0.0



NK Cells IL-2 rest
2.4



Two Way MLR 3 day
0.0



Two Way MLR 5 day
0.0



Two Way MLR 7 day
0.0



PBMC rest
7.8



PBMC PWM
6.7



PBMC PHA-L
0.0



Ramos (B cell) none
6.6



Ramos (B cell) ionomycin
8.8



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
0.0



EOL-1 dbcAMP
100.0



EOL-1 dbcAMP PMA/ionomycin
18.2



Dendritic cells none
0.0



Dendritic cells LPS
0.0



Dendritic cells anti-CD40
0.0



Monocytes rest
3.5



Monocytes LPS
0.0



Macrophages rest
0.0



Macrophages LPS
0.0



HUVEC none
0.0



HUVEC starved
23.3



HUVEC IL-1beta
3.5



HUVEC IFN gamma
0.0



HUVEC TNF alpha + IFN gamma
0.0



HUVEC TNF alpha + IL4
0.0



HUVEC IL-11
3.7



Lung Microvascular EC none
2.8



Lung Microvascular EC TNFalpha + IL-1beta
0.0



Microvascular Dermal EC none
0.0



Microsvasular Dermal EG TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
9.7



Small airway epithelium none
23.0



Small airway epithelium TNFalpha + IL-1beta
0.0



Coronery artery SMC rest
0.0



Coronery artery SMC TNFalpha + IL-1beta
0.0



Astrocytes rest
21.6



Astrocytes TNFalpha + IL-1 beta
4.1



KU-812 (Basophil) rest
0.0



KU-812 (Basophil) PMA/ionomycin
46.3



CCD1106 (Keratinocytes) none
2.5



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
6.9



Liver cirrhosis
16.6



Lupus kidney
2.6



NCI-H292 none
57.8



NCI-H292 IL-4
26.8



NCI-H292 IL-9
54.3



NCI-H292 IL-13
24.0



NCI-H292 IFN gamma
20.7



HPAEC none
0.0



HPAEC TNF alpha + IL-1 beta
0.0



Lung fibroblast none
5.8



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
31.4



Lung fibroblast IL-9
7.6



Lung fibroblast IL-13
3.0



Lung fibroblast IFN gamma
5.3



Dermal fibroblast CCD1070 rest
9.5



Dermal fibroblast CCD1070 TNF alpha
0.0



Dermal fibroblast CCD1070 IL-1 beta
0.0



Dermal fibroblast IFN gamma
0.0



Dermal fibroblast IL-4
0.0



IBD Colitis 2
6.0



IBD Crohn's
1.3



Colon
31.4



Lung
56.3



Thymus
25.3



Kidney
12.2









Column A - Rel. Exp. (%) Ag2502, Run 162293442







Panel 1.3D Summary: Ag2502 Highest expression of this gene was seen in the cerebral cortex (CT=27). In addition, low levels of expression were seen in all CNS regions examined in this panel. This gene encodes a leucine-rich repeat protein. Leucine rich repeats (LRR) mediate reversible protein-protein interactions and have diverse cellular functions, including cellular adhesion and signaling. Several of these proteins, such as connectin, slit, chaoptin, and Toll have pivotal roles in neuronal development in Drosophila and play significant but distinct roles in neural development and in the adult nervous system of humans (Battye R. J. Neurosci. 21: 4290-4298). In Drosophilia, the LRR region of axon guidance proteins are critical for their function , especially in axon repulsion. Since the leucine-rich-repeat protein encoded by this gene was highly expressed in the cerebral cortex, it is a candidate neuronal guidance protein for axons, dendrites and/or growth cones in general. (Itoh A. Brain Res. Mol. Brain Res. 62: 175-186.) Therapeutic modulation of the levels of this protein, or signaling via this protein, are of utility in enhancing/directing compensatory synaptogenesis and fiber growth in the CNS in response to neuronal death (stroke, head trauma), axon lesion (spinal cord injury), or neurodegeneration (Alzheimer's, Parkinson's, Huntington's, vascular dementia or any neurodegenerative disease).


Moderate levels of expression were also seen in cell lines derived from ovarian cancer, lung cancer, and brain cancer. Therapeutic modulation of the expression or function of this gene or gene product is an effective treatment of these cancers.


Among metabolically relevant tissues, this gene was expressed in fetal skeletal muscle, thyroid, and pituitary gland. Therapeutic modulation of this gene or gene product is useful in the treatment of metabolic diseases such as obesity and diabetes, as well as neuroendocrine disorders. Glycoprotein hormones influence the development and function of the ovary, testis and thyroid by binding to specific high-affinity receptors. (Jiang X. Structure 3: 1341-1353). The extracellular domains of these receptors are members of the leucine-rich repeat (LRR) protein superfamily and are responsible for the high-affinity binding.


Panel 2D Summary: Ag2502 Highest expression of this gene was seen in kidney cancer (CTs=27.7). In addition, expression was significantly higher in the kidney cancer samples when compared to expression in samples from the normal adjacent tissue. There was also moderate to low expression in bladder, gastric, colon and ovarian cancers. Expression of this gene is useful as a marker to differentiate the kidney cancer samples from other samples on this panel and as a marker for kidney cancer. Therapeutic targeting of this gene or its protein product with a human monoclonal antibody, small molecule, or protein therapeutic is useful for limiting or blocking the extent of tumor cell migration, invasion, and metastasis, specifically in kidney, ovarian, bladder, gastric, and colon tumors.


Panel 3D Summary: Ag2502 Highest expression of this gene was seen in a lung cancer cell line (CT=28). Moderate levels of expression were seen in a cluster of lung and brain cancer cell lines. Prominent expression was also seen in cerebellum, in agreement with expression seen in Panel 1.3D. Low, but significant expression was also seen in kidney cancer and ovarian cancer cell lines. Expression of this gene is useful as a marker to differentiate lung and brain cancer cell lines and normal brain from other samples on this panel and as a marker for lung and brain cancer. Moderate expression of this gene was also seen in melanoma, rhabdomyosarcoma, osteosarcoma, renal and bladder carcinoma, lymphoma, ovarian and cervical cancer and gastric cancer cell lines. Therapeutic modulation of the expression or function of this gene is useful for the treatment of these cancers.


Panel 4D Summary: Ag2502 Ag2502 Highest expression of this gene was seen in eosinophils (CT=32). Higher levels of gene expression were observed in the eosinophil cell line EOL-1 under resting conditions over the levels in EOL-1 cells stimulated by phorbol ester and ionomycin (CT=34.4). Thus, this gene is involved in eosinophil function. Antibodies raised against this protein that stimulate its activity are useful in the reduction of eosinophil activation and as therapeutic antibodies for the treatment of asthma and allergy and as an anti-inflammatory therapeutics for T cell-mediated autoimmune and inflammatory diseases. Low but significant levels of expression were also seen in a cluster of treated and untreated NCI-H292 mucoepidermoid cells adn in normal colon, lung and thymus. This pattern of restricted expression shows that this gene or gene product is involved in the normal homeostasis of these tissues and/or pathological/inflammatory conditions of the lung.


BF. CG92813-01: Cadherin-related Tumor Suppressor Precursor (FAT)


Expression of gene CG92813-01 was assessed using the primer-probe sets Ag1413, Ag1515, Ag3085, Ag740 and Ag3819, described in Tables BFA, BFB, BFC, BFD, and BFE. Results of the RTQ-PCR runs are shown in Tables BFF, BFG, BFH and BFI.

TABLE BFAProbe Name Ag1413SEQStartIDPrimersSequencesLengthPositionNoForward5′-aggattctggtgttcctcaaat-3′22109081558ProbeTET-5′-tcttccacaggaactgtgcatatcaca-3′-27109311559TAMRAReverse5′-cgagactgtgaaggattgtcat-3′22109711560









TABLE BFB










Probe Name Ag1515
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-ggatggttccatatcagtgaac-3′
22
2078
1561






Probe
TET-5′-ctcgtgaccactgggtcctctgg-3′-TAMRA
23
2108
1562





Reverse
5′-agaacaatctgggaagcaagtt-3′
22
2145
1563
















TABLE BFC










Probe Name Ag3085
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-cttcacctgtagctgccca-3′
19
13801
1564






Probe
TET-5′-acacgggaaggacctgtgagatggt-3′-
25
13827
1565



TAMRA





Reverse
5′-acagaggacgccaagacag-3′
19
13858
1566
















TABLE BFD










Probe Name Ag740
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gagggatattgtcagggtcatc-3′
22
14126
1567






Probe
TET-5′-aaaagcaacgttctcacttccctttt-3′-
26
14100
1568



TAMRA





Reverse
5′-aaatcccaaagaggagaagaaa-3′
22
14062
1569
















TABLE BFE










Probe Name Ag3819
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-agtcatcaatggctcgcata-3′
20
2523
1570






Probe
TET-5′-tcttctggatataaatgataacagccctg-3′-
29
2551
1571



TAMRA





Reverse
5′-aagtattggaccgggtagaaga-3′
22
2580
1572
















TABLE BFF










General_screening_panel_v1.4











Tissue Name
A
B















Adipose
11.3
5.8



Melanoma* Hs688(A).T
84.1
68.8



Melanoma* Hs688(B).T
29.7
20.7



Melanoma* M14
0.0
0.0



Melanoma* LOXIMVI
17.7
14.6



Melanoma* SK-MEL-5
0.7
1.2



Squamous cell carcinoma SCC-4
2.2
2.2



Testis Pool
4.5
4.1



Prostate ca.* (bone met) PC-3
5.0
3.5



Prostate Pool
10.6
7.7



Placenta
2.5
3.7



Uterus Pool
4.6
5.8



Ovarian ca. OVCAR-3
3.6
2.9



Ovarian ca. SK-OV-3
46.0
7.7



Ovarian ca. OVCAR-4
10.4
7.9



Ovarian ca. OVCAR-5
5.8
3.7



Ovarian ca. IGROV-1
1.8
1.5



Ovarian ca. OVCAR-8
3.7
3.3



Ovary
25.2
13.6



Breast ca. MCF-7
0.4
0.2



Breast ca. MDA-MB-231
54.3
40.1



Breast ca. BT 549
32.5
28.7



Breast ca. T47D
7.2
5.6



Breast ca. MDA-N
0.1
0.1



Breast Pool
48.6
15.9



Trachea
8.8
11.3



Lung
6.0
5.9



Fetal Lung
70.7
59.5



Lung ca. NCI-N417
42.9
0.3



Lung ca. LX-1
0.0
0.0



Lung ca. NCI-H146
0.5
0.2



Lung ca. SHP-77
2.8
2.5



Lung ca. A549
1.4
1.7



Lung ca. NCI-H526
0.0
0.0



Lung ca. NCI-H23
100.0
100.0



Lung ca. NCI-H460
44.1
6.5



Lung ca. HOP-62
29.9
20.6



Lung ca. NCI-H522
1.1
1.6



Liver
1.4
0.6



Fetal Liver
16.7
13.5



Liver ca. HepG2
0.4
0.6



Kidney Pool
35.8
24.7



Fetal Kidney
48.6
25.7



Renal ca. 786-0
37.6
30.8



Renal ca. A498
7.6
6.0



Renal ca. ACHN
5.6
3.4



Renal ca. UO-31
37.4
22.7



Renal ca. TK-10
6.4
4.6



Bladder
6.4
4.5



Gastric ca. (liver met.) NCI-N87
0.9
0.5



Gastric ca. KATO III
0.0
0.0



Colon ca. SW-948
0.0
0.0



Colon ca. SW480
0.0
0.0



Colon ca.* (SW480 met) SW620
0.0
0.0



Colon ca. HT29
0.0
0.0



Colon ca. HCT-116
1.3
1.2



Colon ca. CaCo-2
0.1
0.0



Colon cancer tissue
7.4
4.0



Colon ca. SW1116
0.0
0.0



Colon ca. Colo-205
42.3
0.0



Colon ca. SW-48
0.0
0.0



Colon Pool
14.9
11.9



Small Intestine Pool
17.8
16.6



Stomach Pool
48.0
15.0



Bone Marrow Pool
6.3
7.2



Fetal Heart
12.7
8.0



Heart Pool
7.9
6.8



Lymph Node Pool
21.6
19.1



Fetal Skeletal Muscle
9.7
11.2



Skeletal Muscle Pool
5.0
3.9



Spleen Pool
14.8
16.3



Thymus Pool
16.3
16.2



CMS cancer (glio/astro) U87-MG
6.4
6.1



CNS cancer (glio/astro) U-118-MG
48.0
14.8



CNS cancer (neuro; met) SK-N-AS
50.0
28.3



CNS cancer (astro) SF-539
22.7
16.5



CNS cancer (astro) SNB-75
38.7
38.7



CNS cancer (glio) SNB-19
2.7
2.4



CNS cancer (glio) SF-295
27.9
16.8



Brain (Amygdala) Pool
7.1
4.1



Brain (cerebellum)
0.7
0.5



Brain (fetal)
75.3
52.5



Brain (Hippocampus) Pool
7.9
6.2



Cerebral Cortex Pool
4.7
4.2



Brain (Substantia nigra) Pool
4.4
3.2



Brain (Thalamus) Pool
9.0
5.4



Brain (whole)
44.4
4.1



Spinal Cord Pool
6.8
4.5



Adrenal Gland
2.3
2.1



Pituitary gland Pool
1.2
0.9



Salivary Gland
0.9
0.8



Thyroid (female)
7.8
4.7



Pancreatic ca. CAPAN2
5.3
4.5



Pancreas Pool
20.2
14.0









Column A - Rel. Exp. (%) Ag1413, Run 213323517






Column B - Rel. Exp. (%) Ag3819, Run 218713598














TABLE BFG










Panel 2D









Tissue Name
A
B












Normal Colon
68.3
100.0


CC Well to Mod Diff (ODO3866)
6.3
5.0


CC Margin (ODO3866)
7.9
25.7


CC Gr.2 rectosigmoid (ODO3868)
4.6
12.6


CC Margin (ODO3868)
11.0
38.2


CC Mod Diff (ODO3920)
1.4
9.7


CC Margin (ODO3920)
12.1
54.3


CC Gr.2 ascend colon (ODO3921)
20.3
61.6


CC Margin (ODO3921)
10.9
12.9


CC from Partial Hepatectomy (ODO4309) Mets
4.9
3.3


Liver Margin (ODO4309)
10.4
9.0


Colon mets to lung (OD04451-01)
2.9
1.4


Lung Margin (OD04451-02)
10.2
11.5


Normal Prostate 6546-1
2.7
5.1


Prostate Cancer (OD04410)
18.4
12.2


Prostate Margin (OD04410)
36.1
44.4


Prostate Cancer (OD04720-01)
17.3
28.9


Prostate Margin (OD04720-02)
28.5
39.5


Normal Lung 061010
48.0
38.4


Lung Met to Muscle (ODO4286)
3.6
3.0


Muscle Margin (ODO4286)
5.9
3.5


Lung Malignant Cancer (OD03126)
11.7
9.7


Lung Margin (OD03126)
40.3
24.7


Lung Cancer (OD04404)
18.0
2.7


Lung Margin (OD04404)
32.8
9.0


Lung Cancer (OD04565)
2.9
2.7


Lung Margin (OD04565)
8.0
10.2


Lung Cancer (OD04237-01)
4.5
4.0


Lung Margin (OD04237-02)
31.4
17.9


Ocular Mel Met to Liver (ODO4310)
0.9
0.3


Liver Margin (ODO4310)
8.1
10.8


Melanoma Mets to Lung (OD04321)
2.8
1.5


Lung Margin (OD04321)
33.9
19.2


Normal Kidney
100.0
28.5


Kidney Ca, Nuclear grade 2 (OD04338)
14.1
9.8


Kidney Margin (OD04338)
24.0
7.1


Kidney Ca Nuclear grade 1/2 (OD04339)
14.3
6.7


Kidney Margin (OD04339)
22.8
4.5


Kidney Ca, Clear cell type (OD04340)
53.2
16.0


Kidney Margin (OD04340)
26.6
10.5


Kidney Ca, Nuclear grade 3 (OD04348)
8.9
3.6


Kidney Margin (OD04348)
14.1
10.0


Kidney Cancer (OD04622-01)
27.4
27.4


Kidney Margin (OD04622-03)
3.5
4.4


Kidney Cancer (OD04450-01)
8.4
2.6


Kidney Margin (OD04450-03)
23.8
7.5


Kidney Cancer 8120607
1.3
0.4


Kidney Margin 8120608
2.9
1.0


Kidney Cancer 8120613
0.2
0.5


Kidney Margin 8120614
6.3
2.0


Kidney Cancer 9010320
23.0
2.4


Kidney Margin 9010321
11.7
1.7


Normal Uterus
13.8
4.6


Uterus Cancer 064011
17.8
9.3


Normal Thyroid
13.6
10.4


Thyroid Cancer 064010
12.9
6.4


Thyroid Cancer A302152
8.1
5.4


Thyroid Margin A302153
29.1
11.0


Normal Breast
21.0
13.5


Breast Cancer (OD04566)
1.8
1.2


Breast Cancer (OD04590-01)
17.3
1.1


Breast Cancer Mets (OD04590-03)
23.3
3.7


Breast Cancer Metastasis (OD04655-05)
4.2
0.8


Breast Cancer 064006
5.2
3.9


Breast Cancer 1024
12.8
1.8


Breast Cancer 9100266
3.9
1.7


Breast Margin 9100265
10.2
5.0


Breast Cancer A209073
12.2
10.1


Breast Margin A209073
11.7
9.0


Normal Liver
7.1
4.4


Liver Cancer 064003
10.3
8.0


Liver Cancer 1025
5.0
5.1


Liver Cancer 1026
3.0
1.5


Liver Cancer 6004-T
5.4
0.7


Liver Tissue 6004-N
1.4
0.3


Liver Cancer 6005-T
5.2
0.5


Liver Tissue 6005-N
3.3
3.5


Normal Bladder
7.1
7.7


Bladder Cancer 1023
1.0
4.1


Bladder Cancer A302173
3.0
3.6


Bladder Cancer (OD04718-01)
6.0
4.4


Bladder Normal Adjacent (OD04718-03)
27.5
25.2


Normal Ovary
6.7
4.8


Ovarian Cancer 064008
27.0
21.9


Ovarian Cancer (OD04768-07)
0.7
0.2


Ovary Margin (OD04768-08)
12.4
6.7


Normal Stomach
27.4
12.3


Gastric Cancer 9060358
6.9
2.1


Stomach Margin 9060359
7.2
10.9


Gastric Cancer 9060395
29.9
21.0


Stomach Margin 9060394
13.7
16.3


Gastric Cancer 9060397
23.2
18.3


Stomach Margin 9060396
3.7
2.6


Gastric Cancer 064005
18.3
26.8







Column A - Rel. Exp. (%) Ag1413, Run 169477489





Column B - Rel. Exp. (%) Ag740, Run 169590466














TABLE BFH










Panel 4.1D










Tissue Name
A
B
C













Secondary Th1 act
0.0
0.1
0.0


Secondary Th2 act
0.0
0.0
0.0


Secondary Tr1 act
0.0
0.1
0.0


Secondary Th1 rest
0.0
0.1
0.0


Secondary Th2 rest
0.2
0.0
0.0


Secondary Tr1 rest
0.0
0.1
0.0


Primary Th1 act
0.0
0.0
0.0


Primary Th2 act
0.0
0.0
0.0


Primary Tr1 act
0.0
0.0
0.0


Primary Th1 rest
0.0
0.0
0.0


Primary Th2 rest
0.0
0.0
0.0


Primary Tr1 rest
0.0
0.0
0.0


CD45RA CD4 lymphocyte act
3.4
4.1
3.0


CD45RO CD4 lymphocyte act
0.0
0.0
0.0


CD8 lymphocyte act
0.0
0.0
0.0


Secondary CD8 lymphocyte rest
0.0
0.0
0.0


Secondary CD8 lymphocyte act
0.0
0.0
0.0


CD4 lymphocyte none
0.2
0.1
0.0


2ry Th1/Th2/TM anti-CD95 CH11
0.1
0.0
0.0


LAK cells rest
0.3
0.0
0.0


LAK cells IL-2
0.5
0.5
0.0


LAK cells IL-2 + IL-12
0.0
0.1
0.0


LAK cells IL-2 + IFN gamma
0.1
0.3
0.0


LAK cells IL-2 + IL-18
0.5
0.2
0.0


LAK cells PMA/ionomycin
0.1
0.1
0.0


NK Cells IL-2 rest
3.3
2.4
2.2


Two Way MLR 3 day
0.7
0.3
0.0


Two Way MLR 5 day
0.0
0.1
0.0


Two Way MLR 7 day
0.0
0.0
0.0


PBMC rest
0.2
0.2
0.0


PBMC PWM
0.1
0.0
0.0


PBMC PHA-L
0.1
0.0
0.0


Ramos (B cell) none
0.0
0.0
0.0


Ramos (B cell) ionomycin
0.0
0.0
0.0


B lymphocytes PWM
0.0
0.0
0.0


B lymphocytes CD40L and IL-4
0.0
0.2
0.0


EOL-1 dbcAMP
0.0
0.0
0.0


EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0


Dendritic cells none
0.0
0.0
0.0


Dendritic cells LPS
0.0
0.0
0.0


Dendritic cells anti-CD40
0.2
0.0
0.0


Monocytes rest
0.0
0.0
0.0


Monocytes LPS
0.0
0.0
0.0


Macrophages rest
0.0
0.0
0.0


Macrophages LPS
0.0
0.0
0.0


HUVEC none
16.2
17.9
19.5


HUVEC starved
37.1
38.7
49.0


HUVEC IL-1beta
21.3
24.5
21.2


HUVEC IFN gamma
100.0
100.0
100.0


HUVEC TNF alpha + IFN gamma
10.0
19.2
11.8


HUVEC TNF alpha + IL4
4.0
5.2
3.1


HUVEC IL-11
22.5
29.5
28.1


Lung Microvascular EC none
48.6
91.4
59.5


Lung Microvascular EC
12.5
18.8
8.6


TNFalpha + IL-1beta


Microvascular Dermal EC none
62.9
58.6
62.4


Microsvasular Dermal EC
8.8
9.9
9.7


TNFalpha + IL-1beta


Bronchial epithelium TNFalpha +
3.8
4.2
2.7


IL1beta


Small airway epithelium none
1.8
2.1
1.2


Small airway epithelium
3.4
1.9
2.5


TNFalpha + IL-1beta


Coronery artery SMC rest
4.2
3.4
4.4


Coronery artery SMC TNFalpha +
2.0
3.3
4.1


IL-1beta


Astrocytes rest
7.0
6.3
6.0


Astrocytes TNFalpha + IL-1beta
3.7
4.2
6.5


KU-812 (Basophil) rest
1.1
1.5
2.0


KU-812 (Basophil) PMA/ionomycin
4.2
3.9
4.9


CCD1106 (Keratinocytes) none
1.0
3.0
1.7


CCD1106 (Keratinocytes)
1.6
1.3
0.0


TNFalpha + IL-1beta


Liver cirrhosis
4.5
7.0
6.4


NCI-H292 none
0.3
0.9
1.1


NCI-H292 IL-4
0.9
1.3
2.3


NCI-H292 IL-9
2.0
2.9
2.8


NCI-H292 IL-13
1.1
1.0
1.5


NCI-H292 IFN gamma
2.6
2.3
4.2


HPAEC none
27.0
41.5
52.9


HPAEC TNF alpha + IL-1 beta
6.0
7.0
4.0


Lung fibroblast none
10.2
14.4
6.7


Lung fibroblast TNF alpha + IL-1
11.6
10.8
8.9


beta


Lung fibroblast IL-4
9.1
13.9
4.2


Lung fibroblast IL-9
17.3
26.4
12.2


Lung fibroblast IL-13
6.3
13.2
5.4


Lung fibroblast IFN gamma
11.2
9.4
6.6


Dermal fibroblast CCD1070 rest
11.1
15.9
23.2


Dermal fibroblast CCD1070 TNF alpha
4.6
6.3
6.3


Dermal fibroblast CCD1070 IL-1 beta
2.1
3.4
5.9


Dermal fibroblast IFN gamma
6.9
8.4
4.7


Dermal fibroblast IL-4
15.5
17.2
21.8


Dermal Fibroblasts rest
17.6
14.0
12.8


Neutrophils TNFa + LPS
0.0
0.0
0.0


Neutrophils rest
0.0
0.0
0.0


Colon
6.0
5.2
5.5


Lung
17.1
15.1
25.3


Thymus
3.6
2.6
5.3


Kidney
11.4
12.1
14.9







Column A - Rel. Exp. (%) Ag1413, Run 169827815





Column B - Rel. Exp. (%) Ag3819, Run 170127253





Column C - Rel. Exp. (%) Ag740, Run 169827863














TABLE BFI










Panel 4D











Tissue Name
A
B















Secondary Th1 act
0.0
0.0



Secondary Th2 act
0.0
0.0



Secondary Tr1 act
0.0
0.2



Secondary Th1 rest
0.1
0.0



Secondary Th2 rest
0.0
0.0



Secondary Tr1 rest
0.0
0.2



Primary Th1 act
0.0
0.0



Primary Th2 act
0.0
0.0



Primary Tr1 act
0.0
0.0



Primary Th1 rest
0.0
0.0



Primary Th2 rest
0.0
0.0



Primary Tr1 rest
0.0
0.0



CD45RA CD4 lymphocyte act
3.8
4.6



CD45RO CD4 lymphocyte act
0.0
0.3



CD8 lymphocyte act
0.1
0.0



Secondary CD8 lymphocyte rest
0.0
0.0



Secondary CD8 lymphocyte act
0.0
0.0



CD4 lymphocyte none
0.0
0.0



2ry Th1/Th2/Tr1_anti-CD95 CH11
0.1
0.0



LAK cells rest
0.3
0.1



LAK cells IL-2
0.5
0.4



LAK cells IL-2 + IL-12
0.3
0.2



LAK cells IL-2 + IFN gamma
0.4
0.4



LAK cells IL-2 + IL-18
0.5
0.0



LAK cells PMA/ionomycin
0.0
0.4



NK Cells IL-2 rest
2.2
1.8



Two Way MLR 3 day
0.4
0.5



Two Way MLR 5 day
0.1
0.1



Two Way MLR 7 day
0.1
0.0



PBMC rest
0.4
0.6



PBMC PWM
0.4
0.0



PBMC PHA-L
0.1
0.1



Ramos (B cell) none
0.0
0.0



Ramos (B cell) ionomycin
0.0
0.0



B lymphocytes PWM
0.0
0.0



B lymphocytes CD40L and IL-4
0.1
0.0



EOL-1 dbcAMP
0.0
0.0



EOL-1 dbcAMP PMA/ionomycin
0.0
0.0



Dendritic cells none
0.0
0.0



Dendritic cells LPS
0.0
0.0



Dendritic cells anti-CD40
0.0
0.0



Monocytes rest
0.0
0.0



Monocytes LPS
0.0
0.0



Macrophages rest
0.0
0.1



Macrophages LPS
0.0
0.0



HUVEC none
22.8
30.4



HUVEC starved
97.9
88.9



HUVEC IL-1 beta
17.8
8.5



HUVEC IFN gamma
100.0
100.0



HUVEC TNF alpha + IFN gamma
18.2
16.2



HUVEC TNF alpha + IL4
5.4
4.2



HUVEC IL-11
31.4
35.1



Lung Microvascular EC none
50.3
50.7



Lung Microvascular EC TNFalpha + IL-
9.0
6.8



1beta



Microvascular Dermal EC none
71.7
83.5



Microsvasular Dermal EC TNFalpha + IL-
16.2
14.1



1beta



Bronchial epithelium TNFalpha + IL1beta
6.7
5.6



Small airway epithelium none
2.0
2.1



Small airway epithelium TNFalpha + IL-
12.9
8.8



1beta



Coronery artery SMC rest
5.4
4.8



Coronery artery SMC TNFalpha + IL-
2.2
2.6



1beta



Astrocytes rest
5.1
8.9



Astrocytes TNFalpha + IL-1beta
5.8
5.8



KU-812 (Basophil) rest
1.2
0.7



KU-812 (Basophil) PMA/ionomycin
6.7
4.7



CCD1106 (Keratinocytes) none
2.0
4.4



CCD1106 (Keratinocytes) TNFalpha + IL-
1.1
0.7



1beta



Liver cirrhosis
4.8
4.9



Lupus kidney
1.9
4.7



NCI-H292 none
2.1
1.5



NCI-H292 IL-4
3.1
2.3



NCI-H292 IL-9
3.7
3.1



NCI-H292 IL-13
1.5
1.7



NCI-H292 IFN gamma
3.6
1.6



HPAEC none
29.3
40.1



HPAEC TNF alpha + IL-1 beta
4.5
5.3



Lung fibroblast none
12.7
19.1



Lung fibroblast TNF alpha + IL-1 beta
10.0
15.3



Lung fibroblast IL-4
16.4
20.2



Lung fibroblast IL-9
19.1
21.5



Lung fibroblast IL-13
7.5
14.5



Lung fibroblast IFN gamma
17.2
19.6



Dermal fibroblast CCD1070 rest
30.6
27.0



Dermal fibroblast CCD1070 TNF alpha
8.4
10.1



Dermal fibroblast CCD1070 IL-1 beta
6.5
8.5



Dermal fibroblast IFN gamma
7.0
11.0



Dermal fibroblast IL-4
17.6
15.7



IBD Colitis 2
0.5
2.2



IBD Crohn's
1.8
3.0



Colon
14.6
15.6



Lung
17.9
21.0



Thymus
22.1
22.2



Kidney
6.7
5.2









Column A - Rel. Exp. (%) Ag1515, Run 163478545






Column B - Rel. Exp. (%) Ag3085, Run 164682194







General_screening_panel_v1.4 Summary: Ag1413/Ag3819 Highest expression of this gene was seen in a lung cancer cell line NCI-H23 sample (CT=26-28). High to moderate levels of expression of this gene were also seen in a cluster of CNS cancer, renal cancer, lung cancer, breast cancer, ovarian cancer and melanoma cell lines. Therefore, therapeutic modulation of the activity of this gene or its protein product, through the use of small molecule drugs, protein therapeutics or antibodies, is beneficial in the treatment of lung cancer or ovarian cancer. This gene encodes a cadherin-related tumor suppressor precursor. E-cadherin, a related protein is used as a prognostic marker for breast cancer detection (Barshack I, J Clin Pathol 54(9):684-8). Expression of this gene is useful as a diagnostic marker in the above mentioned cancers.


Among tissues with metabolic or endocrine function, this gene was expressed at high to moderate levels in pancreas, adipose, adrenal gland, thyroid, pituitary gland, skeletal muscle, heart, liver and the gastrointestinal tract. Therapeutic modulation of the activity of this gene is useful in the treatment of endocrine/metabolically related diseases, such as obesity and diabetes. In addition, E cadherin, a related protein is shown to be reduced in small intestinal mucosa of coeliac sprue disease (Barshak et. al), a sample not used in this panel. Therapeutic modulation of the activity or function of this gene or gene product is useful in the treatment of coelic sprue disease.


In addition, this gene was expressed at low to moderate levels in all regions of the central nervous system examined, including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. This gene product is a transmembrane glycoproteins belonging to the cadherin superfamily of molecules, which are involved in many biological processes such as cell adhesion, cytoskeletal organization and morphogenesis. Cadherins can act as axon guidance and cell adhesion proteins, specifically during development and in the response to injury (Ranscht B. Int. J. Dev. Neurosci. 18: 643-651.). Therefore, manipulation of levels of this protein are of use in inducing a compensatory synaptogenic response to neuronal death in Alzheimer's disease, Parkinson's disease, Huntington's disease, spinocerebellar ataxia, progressive supranuclear palsy, ALS, head trauma, stroke, or any other disease/condition associated with neuronal loss.


Panel 2D Summary: Ag1413/Ag740 Highest expression of this gene wass detected in samples from normal kidney and colon (CTs=29-30), with significant expression of this gene in both normal and cancer tissues. Expression of this gene was higher in control margin samples of colon (ODO3920), liver (OD04310), and ovary (OD04768-08) as compared to their corresponding cancer tissue. Please see Panel 1.4 for a discussion of the potential utility of this gene.


Panel 4.1D Summary: Ag1413/Ag3819/Ag740 Highest expression of this gene was detected in IFN gamma treated HUVEC cells (CTs=25-27). High to moderate expression of this gene was seen in treated and untreated HUVEC, lung microvascular EC, microvascular dermal EC, Bronchial epithelium, small airway epithelium, NCI-H292, HPAEC, lung fibroblasts, and dermal fibroblasts. The expression of this gene in cells derived from or within the lung shows that this gene is involved in normal conditions as well as pathological and inflammatory lung disorders that include chronic obstructive pulmonary disease, asthma, allergy and emphysema.


In addition, high expression of thisgene was also detected in normal tissues represented by colon, lung, thymus and kidney. Therapeutic modulation of the activity of the protein encoded by this gene is useful in the treatment of inflammatory disease affecting these tissues such as inflammatory bowel disease, chronic obstructive pulmonary disease, asthma, allergy, emphysema, lupus and glomerulonephritis.


Panel 4D Summary: Ag1515/Ag3085 Highest expression of this gene was detected in IFN gamma treated HUVEC cells (CT=25-27). In addition, high to moderate expression of this gene was seen in treated and untreated HVEC, lung microvascular EC, microvascular dermal EC, Bronchial epithelium, small airway epithelium, NCI-H292, HPAEC, lung fibroblasts, and dermal fibroblasts. The expression of this gene in cells derived from or within the lung shows that this gene is involved in normal conditions as well as pathological and inflammatory lung disorders that include chronic obstructive pulmonary disease, asthma, allergy and emphysema.


Expression of this gene was higher in untreated HPAEC (CTs=27-28) as compared to TNF alpha+IL-1 beta treated cells (CTs=30-31). Thus, expression of this gene is useful as a marker to distinguish the treated from untreated HIPAEC samples.


In addition, high levels of expression of this gene were also detected in normal tissues represented by colon, lung, thymus and kidney. Expression of this gene is lower in colon samples from patients with IBD colitis and Crohn's disease relative CTs=31-33) to normal colon (CTs=28-29). Therefore, therapeutic modulation of the activity or function of this gene or the protein encoded by this gene is useful in the treatment of inflammatory bowel disease.


BG. CG93387-05: Fibropellin I Precursor


Expression of gene CG93387-05 was assessed using the primer-probe sets Agl 143, Ag1921, and Ag3082, described in Tables BGA, BGB, and BGC. Results of the RTQ-PCR runs are shown in Tables BGD, BGE, and BGF.

TABLE BGAProbe Name Ag1143SEQStartIDPrimersSequencesLengthPositionNoForward5′-gagatggatgtggacgactg-3′209641573ProbeTET-5′-cctgaatggaggctcttgtgttgacc-3′-269991574TAMRAReverse5′-acaagcaggtgtaattcccc-3′2010291575









TABLE BGB










Probe Name Ag1921
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-acacattccagactgtgctcat-3′
22
317
1576






Probe
TET-5′-acggcaagctctccttcaccatctt-3′-
25
344
1577



TAMRA





Reverse
5′-tccacacgatggactcatagtt-3′
22
370
1578
















TABLE BGC










Probe Name Ag3082
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-cagacgcagaggtagctcc-3′
19
1385
1579






Probe
TET-5′-catctgggcactgtgtgttcatgttg-3′-
26
1335
1580



TAMRA





Reverse
5′-atttgaaatcacagccatgc-3′
20
1311
1581
















TABLE BGD










Panel 1.3D










Tissue Name
A














Liver adenocarcinoma
31.2



Pancreas
2.5



Pancreatic ca. CAPAN 2
0.2



Adrenal gland
12.3



Thyroid
19.5



Salivary gland
2.3



Pituitary gland
10.2



Brain (fetal)
2.1



Brain (whole)
10.8



Brain (amygdala)
12.3



Brain (cerebellum)
21.0



Brain (hippocampus)
21.8



Brain (substantia nigra)
3.9



Brain (thalamus)
8.7



Cerebral Cortex
8.7



Spinal cord
12.8



glio/astro U87-MG
3.0



glio/astro U-118-MG
100.0



astrocytoma SW1783
6.9



neuro*; met SK-N-AS
3.1



astrocytoma SF-539
12.6



astrocytoma SNB-75
25.9



glioma SNB-19
8.9



glioma U251
4.9



glioma SF-295
33.7



Heart (Fetal)
1.6



Heart
5.8



Skeletal muscle (Fetal)
5.8



Skeletal muscle
14.3



Bone marrow
1.0



Thymus
5.1



Spleen
22.5



Lymph node
28.5



Colorectal
3.6



Stomach
11.8



Small intestine
14.5



Colon ca. SW480
0.8



Colon ca.* SW620 (SW480 met)
0.3



Colon ca. HT29
0.0



Colon ca. HCT-116
1.1



Colon ca. CaCo-2
0.0



CC Well to Mod Diff (ODO3866)
3.1



Colon ca. HCC-2998
0.4



Gastric ca. (liver met) NCI-N87
3.5



Bladder
8.8



Trachea
7.5



Kidney
3.5



Kidney (fetal)
3.4



Renal ca. 786-0
0.2



Renal ca. A498
12.4



Renal ca. RXF 393
0.0



Renal ca. ACHN
0.0



Renal ca. UO-31
0.2



Renal ca. TK-10
0.1



Liver
2.4



Liver (fetal)
1.6



Liver ca. (hepatoblast) HepG2
0.0



Lung
9.0



Lung (fetal)
6.4



Lung ca. (small cell) LX-1
2.9



Lung ca. (small cell) NCI-H69
0.3



Lung ca. (s. cell var.) SHP-77
4.0



Lung ca. (large cell)NCI-H460
4.4



Lung ca. (non-sm. cell) A549
1.3



Lung ca. (non-s. cell) NCI-H23
2.9



Lung ca. (non-s. cell) HOP-62
2.7



Lung ca. (non-s. cl) NCI-H522
0.0



Lung ca. (squam.) SW 900
6.0



Lung ca. (squam.) NCI-H596
0.8



Mammary gland
12.5



Breast ca.* (pl. ef) MCF-7
0.3



Breast ca.* (pl. ef) MDA-MB-231
1.7



Breast ca.* (pl. ef) T47D
1.0



Breast ca. BT-549
15.1



Breast ca. MDA-N
0.5



Ovary
13.4



Ovarian ca. OVCAR-3
1.1



Ovarian ca. OVCAR-4
0.0



Ovarian ca. OVCAR-5
12.2



Ovarian ca. OVCAR-8
0.5



Ovarian ca. IGROV-1
0.0



Ovarian ca. (ascites) SK-OV-3
7.2



Uterus
32.8



Placenta
1.1



Prostate
12.4



Prostate ca.* (bone met) PC-3
0.0



Testis
8.1



Melanoma Hs688(A).T
2.6



Melanoma* (met) Hs688(B).T
0.9



Melanoma UACC-62
4.8



Melanoma M14
17.8



Melanoma LOX IMVI
0.2



Melanoma* (met) SK-MEL-5
0.2



Adipose
17.2









Column A - Rel. Exp. (%) Ag3082, Run 165673172














TABLE BGE










Panel 2.2










Tissue Name
A














Normal Colon
20.3



Colon cancer (OD06064)
8.5



Colon Margin (OD06064)
6.4



Colon cancer (OD06159)
1.4



Colon Margin (OD06159)
9.6



Colon cancer (OD06297-04)
1.1



Colon Margin (OD06297-05)
5.7



CC Gr.2 ascend colon (ODO3921)
5.9



CC Margin (ODO3921)
2.9



Colon cancer metastasis (OD06104)
1.3



Lung Margin (OD06104)
0.8



Colon mets to lung (OD04451-01)
17.3



Lung Margin (OD04451-02)
17.2



Normal Prostate
20.3



Prostate Cancer (OD04410)
13.1



Prostate Margin (OD04410)
10.1



Normal Ovary
52.5



Ovarian cancer (OD06283-03)
8.6



Ovarian Margin (OD06283-07)
13.1



Ovarian Cancer
23.2



Ovarian cancer (OD06145)
6.4



Ovarian Margin (OD06145)
52.5



Ovarian cancer (OD06455-03)
13.6



Ovarian Margin (OD06455-07)
33.2



Normal Lung
19.6



Invasive poor diff. lung adeno (ODO4945-01
7.7



Lung Margin (ODO4945-03)
48.3



Lung Malignant Cancer (OD03126)
11.0



Lung Margin (OD03126)
6.5



Lung Cancer (OD05014A)
11.8



Lung Margin (OD05014B)
49.0



Lung cancer (OD06081)
17.6



Lung Margin (OD06081)
37.9



Lung Cancer (OD04237-01)
1.2



Lung Margin (OD04237-02)
23.7



Ocular Mel Met to Liver (ODO4310)
44.1



Liver Margin (ODO4310)
14.9



Melanoma Metastasis
0.0



Lung Margin (OD04321)
16.3



Normal Kidney
9.8



Kidney Ca, Nuclear grade 2 (OD04338)
26.6



Kidney Margin (OD04338)
1.6



Kidney Ca Nuclear grade 1/2 (OD04339)
20.2



Kidney Margin (OD04339)
10.2



Kidney Ca, Clear cell type (OD04340)
20.9



Kidney Margin (OD04340)
13.3



Kidney Ca, Nuclear grade 3 (OD04348)
4.4



Kidney Margin (OD04348)
54.0



Kidney malignant cancer (OD06204B)
0.6



Kidney normal adjacent tissue (OD06204E)
8.5



Kidney Cancer (OD04450-01)
0.5



Kidney Margin (OD04450-03)
16.4



Kidney Cancer 8120613
0.0



Kidney Margin 8120614
11.0



Kidney Cancer 9010320
1.5



Kidney Margin 9010321
7.1



Kidney Cancer 8120607
6.3



Kidney Margin 8120608
5.9



Normal Uterus
52.1



Uterine Cancer 064011
37.6



Normal Thyroid
10.2



Thyroid Cancer
4.0



Thyroid Cancer A302152
21.0



Thyroid Margin A302153
29.5



Normal Breast
52.1



Breast Cancer
2.7



Breast Cancer
43.5



Breast Cancer (OD04590-01)
59.5



Breast Cancer Mets (OD04590-03)
35.4



Breast Cancer Metastasis
44.1



Breast Cancer
17.9



Breast Cancer 9100266
16.6



Breast Margin 9100265
26.1



Breast Cancer A209073
9.8



Breast Margin A209073
28.3



Breast cancer (OD06083)
63.7



Breast cancer node metastasis (OD06083)
100.0



Normal Liver
39.8



Liver Cancer 1026
17.2



Liver Cancer 1025
40.3



Liver Cancer 6004-T
33.7



Liver Tissue 6004-N
2.2



Liver Cancer 6005-T
42.3



Liver Tissue 6005-N
44.8



Liver Cancer
4.2



Normal Bladder
17.6



Bladder Cancer
8.4



Bladder Cancer
3.2



Normal Stomach
25.2



Gastric Cancer 9060397
0.7



Stomach Margin 9060396
4.7



Gastric Cancer 9060395
8.1



Stomach Margin 9060394
12.2



Gastric Cancer 064005
7.2









Column A - Rel. Exp. (%) Ag3082, Run 174284798














TABLE BGF










Panel 4D












Tissue Name
A
B
C
















Secondary Th1 act
1.3
0.0
1.4



Secondary Th2 act
6.3
0.5
5.8



Secondary Tr1 act
5.5
0.7
3.8



Secondary Th1 rest
6.3
1.9
12.8



Secondary Th2 rest
17.0
2.0
19.2



Secondary Tr1 rest
12.9
0.8
18.8



Primary Th1 act
0.9
0.0
0.8



Primary Th2 act
0.7
0.0
2.3



Primary Tr1 act
1.5
0.1
2.3



Primary Th1 rest
21.0
5.8
50.7



Primary Th2 rest
32.5
7.5
0.0



Primary Tr1 rest
2.3
0.3
6.3



CD45RA CD4 lymphocyte act
2.5
1.4
2.3



CD45RO CD4 lymphocyte act
2.7
1.1
1.8



CD8 lymphocyte act
2.1
0.6
2.9



Secondary CD8
2.9
0.5
4.2



lymphocyte rest



Secondary CD8
5.4
0.0
3.1



lymphocyte act



CD4 lymphocyte none
11.7
3.7
10.8



2ry Th1/Th2/Tr1 anti-
27.5
1.3
27.0



CD95 CH11



LAK cells rest
4.5
1.1
4.4



LAK cells IL-2
6.8
3.7
11.3



LAK cells IL-2 + IL-12
1.8
0.3
2.8



LAK cells IL-2 + IFN
3.2
1.1
6.4



gamma



LAK cells IL-2 + IL-18
1.7
0.5
7.6



LAK cells PMA/ionomycin
1.6
0.1
1.1



NK Cells IL-2 rest
2.2
0.8
3.2



Two Way MLR 3 day
5.6
2.3
6.0



Two Way MLR 5 day
1.1
0.4
1.4



Two Way MLR 7 day
0.9
0.5
1.4



PBMC rest
7.1
1.7
4.6



PBMC PWM
0.9
1.0
3.8



PBMC PHA-L
3.0
0.4
2.6



Ramos (B cell) none
0.0
0.0
0.0



Ramos (B cell) ionomycin
0.0
0.0
0.2



B lymphocytes PWM
5.3
2.0
22.8



B lymphocytes CD40L
18.0
3.6
57.8



and IL-4



EOL-1 dbcAMP
0.0
0.1
0.2



EOL-1 dbcAMP PMA/ionomycin
0.0
0.0
0.0



Dendritic cells none
3.3
4.2
5.1



Dendritic cells LPS
0.8
1.5
1.3



Dendritic cells anti-CD40
4.3
4.6
5.4



Monocytes rest
2.4
8.6
6.3



Monocytes LPS
0.0
0.2
1.0



Macrophages rest
1.8
2.9
1.7



Macrophages LPS
0.7
0.5
0.5



HUVEC none
6.2
12.1
10.4



HUVEC starved
4.4
11.9
12.9



HUVEC IL-1beta
0.9
1.6
3.3



HUVEC IFN gamma
23.0
13.6
13.6



HUVEC TNF alpha + IFN gamma
2.1
3.5
7.5



HUVEC TNF alpha + IL4
6.2
4.2
3.2



HUVEC IL-11
12.1
8.7
8.1



Lung Microvascular EC none
27.7
24.3
21.5



Lung Microvascular EC TNFalpha +
8.7
14.5
16.6



IL-1beta



Microvascular Dermal EC none
19.5
19.3
20.3



Microsvasular Dermal EC
10.1
11.3
10.3



TNFalpha + IL-1beta



Bronchial epithelium TNFalpha +
0.3
0.4
1.0



IL1beta



Small airway epithelium none
2.0
4.9
2.6



Small airway epithelium TNFalpha +
0.7
0.2
2.1



IL-1beta



Coronery artery SMC rest
0.7
3.6
2.9



Coronery artery SMC TNFalpha +
6.7
1.4
3.7



IL-1beta



Astrocytes rest
3.7
3.0
3.7



Astrocytes TNFalpha + IL-1beta
3.7
3.4
4.0



KU-812 (Basophil) rest
0.0
0.0
0.0



KU-812 (Basophil) PMA/ionomycin
0.0
0.1
0.1



CCD1106 (Keratinocytes) none
0.0
0.0
0.1



93580 CCD1106 (Keratinocytes)
0.0
0.0
0.2



TNFa and IFNg



Liver cirrhosis
3.5
4.5
5.9



Lupus kidney
15.0
6.1
10.2



NCI-H292 none
0.4
1.6
3.5



NCI-H292 IL-4
0.6
1.3
3.4



NCI-H292 IL-9
1.0
2.2
3.0



NCI-H292 IL-13
3.5
0.4
0.8



NCI-H292 IFN gamma
2.3
1.0
1.3



HPAEC none
58.6
32.5
32.1



HPAEC TNF alpha + IL-1 beta
18.6
16.8
22.4



Lung fibroblast none
78.5
79.6
90.1



Lung fibroblast TNF alpha + IL-1
20.0
18.0
24.0



beta



Lung fibroblast IL-4
48.3
100.0
100.0



Lung fibroblast IL-9
35.4
56.6
49.7



Lung fibroblast IL-13
100.0
64.2
77.4



Lung fibroblast IFN gamma
56.6
96.6
82.4



Dermal fibroblast CCD1070 rest
5.7
9.3
17.2



Dermal fibroblast CCD1070 TNF
11.3
6.0
25.5



alpha



Dermal fibroblast CCD1070 IL-1
20.3
4.0
6.5



beta



Dermal fibroblast IFN gamma
30.8
9.2
18.7



Dermal fibroblast IL-4
35.1
17.8
26.4



IBD Colitis 2
4.1
2.8
5.5



IBD Crohn's
1.7
1.4
2.2



Colon
6.2
7.4
8.5



Lung
13.9
19.1
20.3



Thymus
25.2
16.4
14.7



Kidney
13.8
8.6
31.0









Column A - Rel. Exp. (%) Ag1143, Run 139943479






Column B - Rel. Exp. (%) Ag1921, Run 164629443






Column C - Rel. Exp. (%) Ag3082, Run 164681898







Panel 1.3D Summary: Ag3082 Highest expression of this gene was seen in a brain cancer cell line (CT=27.3). Significant levels of expression were also seen in a cluster of samples derived from ovarian, breast, melanoma and brain cancer cell lines. Thus, expression of this gene is useful as a marker to differentiate between the brain cancer samples and other samples on this panel and as a marker to detect the presence of these cancers. This gene encodes a protein that is homologous to an epidermal growth factor related protein (fibropellin like). Fibropellins are a family of extracellular sea urchin matrix proteins that have been implicated in cell adhestion. Therefore, therapeutic modulation of the expression or function of this gene is useful in the treatment of ovarian, breast, melanoma and brain cancers.


Among tissues with metabolic function, this gene was expressed at moderate to low levels in pituitary, adipose, adrenal gland, pancreas, thyroid, and adult and fetal skeletal muscle, heart, and liver. This widespread expression among these tissues shows that this gene product plays a role in normal neuroendocrine and metabolic and that disregulated expression of this gene contributes to neuroendocrine disorders or metabolic diseases, such as obesity and diabetes.


Moderate to low levels of expression were also seen in all regions of the CNS examined, including pituitary, amygdala, thalamus, substantia nigra, cerebral cortex, and hippocampus. Therefore, therapeutic modulation of the expression or function of this gene is useful in the treatment of neurologic disorders, such as Alzheimer's disease, Parkinson's disease, schizophrenia, multiple sclerosis, stroke and epilepsy.


Panel 2.2 Summary: Ag3082 Highest expression of this gene was seen in a breast cancer metastasis (CT=28.3). Significant levels of expression were also seen in a cluster of breast cancer samples. Conversely, expression was higher in normal ovary and lung tissue when compared to expression in the normal adjacent tissue. Thus, therapeutic modulation of the expression or function of this gene is effective in the treatment of breast, ovarian and lung cancers.


Panel 4D Summary: Agl 143/Ag1921/Ag3082 Highest expression of this gene was detected in treated lung fibroblasts (CTs=27-29). Moderate levels of expression were also seen in treated dermal fibroblasts, and lung and dermal microvasculature, and HUVECs. Thus, expression of this gene is useful as a marker of fibroblasts or vasculature. The protein encoded by the transcript plays an important role in the normal homeostasis of these tissues. Therefore, therapeutics designed with this gene or gene product are important for maintaining or restoring normal function to these organs during inflammation associated with asthma, psoriasis, and emphysema.


BH. CG93871-01 and CG93871-05: Weakly Similar to Fibulin-1


Expression of gene CG93871-05 and variant CG93871-01 was assessed using the primer-probe sets Ag1294b, Ag746, and Ag4726, described in Tables BHA, BHB, and BHC. Results of the RTQ-PCR runs are shown in Tables BHD, BHE, BHF, BHG, and BHH. Please note that the probe and primer set Ag4726 is specific to the CG93871-05 variant only.

TABLE BHAProbe Name Ag1294bSEQStartIDPrimersSequencesLengthPositionNoForward5′-cattggcagctacaagtgttc-3′215321582ProbeTET-5′-ctgtcgaactggcttccaccttcat-3′-255531583TAMRAReverse5′-cctccgacactcgtttacatc-3′215991584









TABLE BHB










Probe Name Ag746
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gcattggcagctacaagtgt-3′
20
531
1585






Probe
TET-5′-ctgtcgaactggcttccaccttcat-3′-
25
553
1586



TAMRA





Reverse
5′-cctccgacactcgtttacatc-3′
21
599
1587
















TABLE BHC










Probe Name Ag4726
















SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gtgtctgtctggctggaaac-3′
20
1338
1588






Probe
TET-5′-tgcatctctcctgagtgtccttctgg-3′-
26
1364
1589



TAMRA





Reverse
5′-acaagtacagcaatccgtctgt-3′
22
1408
1590
















TABLE BHD










AI_comprehensive panel_v1.0










Tissue Name
A














110967 COPD-F
6.6



110980 COPD-F
16.6



110968 COPD-M
3.9



110977 COPD-M
31.6



110989 Emphysema-F
45.1



110992 Emphysema-F
7.2



110993 Emphysema-F
5.8



110994 Emphysema-F
3.3



110995 Emphysema-F
2.0



110996 Emphysema-F
3.1



110997 Asthma-M
3.7



111001 Asthma-F
2.8



111002 Asthma-F
5.3



111003 Atopic Asthma-F
6.1



111004 Atopic Asthma-F
3.4



111005 Atopic Asthma-F
3.9



111006 Atopic Asthma-F
2.4



111417 Allergy-M
6.6



112347 Allergy-M
3.3



112349 Normal Lung-F
3.2



112357 Normal Lung-F
100.0



112354 Normal Lung-M
58.6



112374 Crohns-F
7.5



112389 Match Control Crohns-F
3.5



112375 Crohns-F
5.1



112732 Match Control Crohns-F
0.5



112725 Crohns-M
10.6



112387 Match Control Crohns-M
3.5



112378 Crohns-M
1.7



112390 Match Control Crohns-M
55.5



112726 Crohns-M
3.6



112731 Match Control Crohns-M
13.9



112380 Ulcer Col-F
13.7



112734 Match Control Ulcer Col-F
5.6



112384 Ulcer Col-F
3.9



112737 Match Control Ulcer Col-F
3.3



112386 Ulcer Col-F
0.0



112738 Match Control Ulcer Col-F
0.0



112381 Ulcer Col-M
4.2



112735 Match Control Ulcer Col-M
18.2



112382 Ulcer Col-M
4.2



112394 Match Control Ulcer Col-M
0.0



112383 Ulcer Col-M
12.2



112736 Match Control Ulcer Col-M
2.0



112423 Psoriasis-F
3.9



112427 Match Control Psoriasis-F
30.8



112418 Psoriasis-M
4.6



112723 Match Control Psoriasis-M
23.8



112419 Psoriasis-M
2.7



112424 Match Control Psoriasis-M
1.9



112420 Psoriasis-M
4.9



112425 Match Control Psoriasis-M
25.9



104689 (MF) OA Bone-Backus
12.9



104690 (MF) Adj “Normal” Bone-Backus
3.7



104691 (MF) OA Synovium-Backus
6.9



104692 (BA) OA Cartilage-Backus
21.3



104694 (BA) OA Bone-Backus
6.6



104695 (BA) Adj “Normal” Bone-Backus
2.3



104696 (BA) OA Synovium-Backus
5.7



104700 (SS) OA Bone-Backus
6.2



104701 (SS) Adj “Normal” Bone-Backus
3.8



104702 (SS) OA Synovium-Backus
15.4



117093 OA Cartilage Rep7
18.0



112672 OA Bone5
90.1



112673 OA Synovium5
63.7



112674 OA Synovial Fluid cells5
32.3



117100 OA Cartilage Rep14
3.3



112756 OA Bone9
7.0



112757 OA Synovium9
12.2



112758 OA Synovial Fluid Cells9
3.9



117125 RA Cartilage Rep2
4.6



113492 Bone2 RA
2.4



113493 Synovium2 RA
1.1



113494 Syn Fluid Cells RA
1.4



113499 Cartilage4 RA
1.4



113500 Bone4 RA
0.5



113501 Synovium4 RA
1.7



113502 Syn Fluid Cells4 RA
1.8



113495 Cartilage3 RA
1.6



113496 Bone3 RA
1.1



113497 Synovium3 RA
0.0



113498 Syn Fluid Cells3 RA
0.6



117106 Normal Cartilage Rep20
4.5



113663 Bone3 Normal
6.7



113664 Synovium3 Normal
1.2



113665 Syn Fluid Cells3 Normal
0.9



117107 Normal Cartilage Rep22
1.3



113667 Bone4 Normal
11.8



113668 Synovium4 Normal
12.0



113669 Syn Fluid Cells4 Normal
10.7









Column A - Rel. Exp. (%) Ag1294b, Run 249007981














TABLE BHE










General_screening_panel_v1.4










Tissue Name
A














Adipose
3.3



Melanoma* Hs688(A).T
2.7



Melanoma* Hs688(B).T
2.7



Melanoma* M14
4.8



Melanoma* LOXIMVI
0.1



Melanoma* SK-MEL-5
0.4



Squamous cell carcinoma SCC-4
0.2



Testis Pool
4.3



Prostate ca.* (bone met) PC-3
1.6



Prostate Pool
0.5



Placenta
7.7



Uterus Pool
0.1



Ovarian ca. OVCAR-3
0.7



Ovarian ca. SK-OV-3
0.6



Ovarian ca. OVCAR-4
0.3



Ovarian ca. OVCAR-5
1.1



Ovarian ca. IGROV-1
1.4



Ovarian ca. OVCAR-8
0.7



Ovary
5.0



Breast ca. MCF-7
0.4



Breast ca. MDA-MB-231
0.5



Breast ca. BT 549
0.7



Breast ca. T47D
4.2



Breast ca. MDA-N
0.2



Breast Pool
0.8



Trachea
1.3



Lung
5.5



Fetal Lung
1.8



Lung ca. NCI-N417
3.6



Lung ca. LX-1
0.7



Lung ca. NCI-H146
0.8



Lung ca. SHP-77
0.3



Lung ca. A549
0.8



Lung ca. NCI-H526
2.1



Lung ca. NCI-H23
0.8



Lung ca. NCI-H460
1.2



Lung ca. HOP-62
0.5



Lung ca. NCI-H522
20.2



Liver
11.6



Fetal Liver
61.1



Liver ca. HepG2
100.0



Kidney Pool
6.7



Fetal Kidney
2.0



Renal ca. 786-0
1.7



Renal ca. A498
1.3



Renal ca. ACHN
2.5



Renal ca. UO-31
10.6



Renal ca. TK-10
41.8



Bladder
1.8



Gastric ca. (liver met.) NCI-N87
2.0



Gastric ca. KATO III
0.6



Colon ca. SW-948
0.6



Colon ca. SW480
0.7



Colon ca.* (SW480 met) SW620
12.8



Colon ca. HT29
0.1



Colon ca. HCT-116
3.7



Colon ca. CaCo-2
31.9



Colon cancer tissue
7.9



Colon ca. SW1116
1.0



Colon ca. Colo-205
0.0



Colon ca. SW-48
0.0



Colon Pool
1.0



Small Intestine Pool
4.9



Stomach Pool
3.4



Bone Marrow Pool
0.0



Fetal Heart
0.7



Heart Pool
0.7



Lymph Node Pool
2.6



Fetal Skeletal Muscle
1.6



Skeletal Muscle Pool
1.0



Spleen Pool
0.4



Thymus Pool
7.9



CNS cancer (glio/astro) U87-MG
6.4



CNS cancer (glio/astro) U-118-MG
1.6



CNS cancer (neuro; met) SK-N-AS
4.4



CNS cancer (astro) SF-539
0.2



CNS cancer (astro) SNB-75
4.4



CNS cancer (glio) SNB-19
1.1



CNS cancer (glio) SF-295
5.1



Brain (Amygdala) Pool
2.5



Brain (cerebellum)
7.3



Brain (fetal)
12.2



Brain (Hippocampus) Pool
1.9



Cerebral Cortex Pool
2.6



Brain (Substantia nigra) Pool
2.1



Brain (Thalamus) Pool
3.9



Brain (whole)
8.5



Spinal Cord Pool
1.9



Adrenal Gland
2.6



Pituitary gland Pool
0.6



Salivary Gland
0.9



Thyroid (female)
1.8



Pancreatic ca. CAPAN2
0.0



Pancreas Pool
1.1









Column A - Rel. Exp. (%) Ag4726, Run 222842378














TABLE BHF










Panel 1.2











Tissue Name
A
B















Endothelial cells
12.3
5.9



Heart (Fetal)
0.0
0.0



Pancreas
0.0
0.0



Pancreatic ca. CAPAN 2
0.0
0.0



Adrenal gland
0.0
0.2



Thyroid
0.1
0.0



Salivary gland
0.0
0.0



Pituitary gland
0.2
0.1



Brain (fetal)
2.4
16.0



Brain (whole)
0.0
0.3



Brain (amygdala)
0.0
0.0



Brain (cerebellum)
0.0
0.0



Brain (hippocampus)
0.0
0.0



Brain (thalamus)
0.0
0.0



Cerebral Cortex
0.0
0.0



Spinal cord
0.0
0.0



glio/astro U87-MG
0.0
0.0



glio/astro U-118-MG
0.0
0.0



astrocytoma SW1783
0.0
0.0



neuro*; met SK-N-AS
0.0
0.2



astrocytoma SF-539
0.0
0.0



astrocytoma SNB-75
0.0
0.0



glioma SNB-19
0.0
0.0



glioma U251
0.0
0.0



glioma SF-295
0.0
0.0



Heart
0.0
0.0



Skeletal muscle
0.0
0.0



Bone marrow
0.0
0.0



Thymus
1.2
2.8



Spleen
0.0
0.0



Lymph node
0.0
0.0



Colorectal
0.0
0.0



Stomach
0.0
0.0



Small intestine
0.0
0.0



Colon ca. SW480
0.0
0.0



Colon ca.* SW620 (SW480 met)
1.1
1.9



Colon ca. HT29
0.0
0.0



Colon ca. HCT-116
0.0
0.0



Colon ca. CaCo-2
46.3
56.6



CC Well to Mod Diff (ODO3866)
0.0
0.0



Colon ca. HCC-2998
0.0
0.0



Gastric ca. (liver met) NCI-N87
0.0
0.0



Bladder
0.0
0.0



Trachea
0.0
0.0



Kidney
0.0
0.0



Kidney (fetal)
0.1
0.9



Renal ca. 786-0
0.0
0.0



Renal ca. A498
0.0
0.0



Renal ca. RXF 393
0.0
0.0



Renal ca. ACHN
0.0
0.0



Renal ca. UO-31
0.0
0.0



Renal ca. TK-10
0.0
0.0



Liver
32.8
53.2



Liver (fetal)
72.7
100.0



Liver ca. (hepatoblast) HepG2
100.0
94.0



Lung
0.0
0.0



Lung (fetal)
0.0
0.0



Lung ca. (small cell) LX-1
0.0
0.0



Lung ca. (small cell) NCI-H69
0.0
0.0



Lung ca. (s. cell var.) SHP-77
0.0
0.0



Lung ca. (large cell) NCI-H460
0.0
0.0



Lung ca. (non-sm. cell) A549
0.0
0.0



Lung ca. (non-s. cell) NCI-H23
0.0
0.0



Lung ca. (non-s. cell) HOP-62
0.0
0.0



Lung ca. (non-s. cl) NCI-H522
63.7
90.1



Lung ca. (squam.) SW 900
0.0
0.0



Lung ca. (squam.) NCI-H596
0.0
0.0



Mammary gland
0.7
3.6



Breast ca.* (pl. ef) MCF-7
0.0
0.0



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0



Breast ca.* (pl. ef) T47D
0.0
0.0



Breast ca. BT-549
0.0
0.0



Breast ca. MDA-N
0.0
0.0



Ovary
0.5
11.7



Ovarian ca. OVCAR-3
0.0
0.0



Ovarian ca. OVCAR-4
0.0
0.0



Ovarian ca. OVCAR-5
0.0
0.0



Ovarian ca. OVCAR-8
0.0
0.0



Ovarian ca. IGROV-1
0.0
0.0



Ovarian ca. (ascites) SK-OV-3
0.0
0.0



Uterus
0.0
0.0



Placenta
34.4
39.5



Prostate
0.0
0.0



Prostate ca.* (bone met) PC-3
0.0
0.0



Testis
1.0
3.5



Melanoma Hs688(A).T
0.0
0.0



Melanoma* (met) Hs688(B).T
0.0
0.0



Melanoma UACC-62
0.0
0.0



Melanoma M14
0.0
0.0



Melanoma LOX IMVI
0.0
0.0



Melanoma* (met) SK-MEL-5
0.0
0.0









Column A - Rel. Exp. (%) Ag746, Run 115163442






Column B - Rel. Exp. (%) Ag746, Run 119442272














TABLE BHG










Panel 2D











Tissue Name
A
B















Normal Colon
18.3
21.8



CC Well to Mod Diff (ODO3866)
16.5
23.7



CC Margin (ODO3866)
3.1
0.0



CC Gr.2 rectosigmoid (ODO3868)
0.0
0.8



CC Margin (ODO3868)
0.5
2.0



CC Mod Diff (ODO3920)
1.2
2.3



CC Margin (ODO3920)
1.3
2.6



CC Gr.2 ascend colon (ODO3921)
3.4
4.4



CC Margin (ODO3921)
1.3
0.0



CC from Partial Hepatectomy
8.4
1.9



(ODO4309) Mets



Liver Margin (ODO4309)
49.7
41.5



Colon mets to lung (OD04451-01)
0.3
5.3



Lung Margin (OD04451-02)
0.0
1.8



Normal Prostate 6546-1
9.1
12.1



Prostate Cancer (OD04410)
2.0
9.7



Prostate Margin (OD04410)
16.8
20.3



Prostate Cancer (OD04720-01)
13.5
14.4



Prostate Margin (OD04720-02)
14.0
22.4



Normal Lung
6.8
11.7



Lung Met to Muscle (ODO4286)
1.8
0.7



Muscle Margin (ODO4286)
11.5
13.1



Lung Malignant Cancer (OD03126)
1.5
6.0



Lung Margin (OD03126)
4.8
2.4



Lung Cancer (OD04404)
4.2
2.3



Lung Margin (OD04404)
9.0
10.4



Lung Cancer (OD04565)
0.3
0.0



Lung Margin (OD04565)
0.4
0.3



Lung Cancer (OD04237-01)
10.7
11.1



Lung Margin (OD04237-02)
4.9
5.4



Ocular Mel Met to Liver (ODO4310)
10.5
11.9



Liver Margin (ODO4310)
22.4
32.8



Melanoma Metastasis
0.0
0.0



Lung Margin (OD04321)
0.6
0.0



Normal Kidney
5.3
5.3



Kidney Ca, Nuclear grade 2 (OD04338)
39.8
43.8



Kidney Margin (OD04338)
4.8
6.4



Kidney Ca Nuclear grade 1/2
3.0
0.3



(OD04339)



Kidney Margin (OD04339)
5.4
10.0



Kidney Ca, Clear cell type (OD04340)
18.2
19.2



Kidney Margin (OD04340)
9.0
10.4



Kidney Ca, Nuclear grade 3 (OD04348)
5.2
8.3



Kidney Margin (OD04348)
6.9
4.7



Kidney Cancer (OD04622-01)
41.8
45.4



Kidney Margin (OD04622-03)
1.9
1.4



Kidney Cancer (OD04450-01)
9.2
6.2



Kidney Margin (OD04450-03)
10.2
9.0



Kidney Cancer 8120607
2.2
1.7



Kidney Margin 8120608
6.5
6.4



Kidney Cancer 8120613
2.2
0.7



Kidney Margin 8120614
6.3
3.0



Kidney Cancer 9010320
10.9
16.5



Kidney Margin 9010321
9.0
11.3



Normal Uterus
4.3
6.3



Uterine Cancer 064011
13.4
17.7



Normal Thyroid
9.1
14.9



Thyroid Cancer
6.4
5.9



Thyroid Cancer A302152
4.4
5.1



Thyroid Margin A302153
12.0
22.1



Normal Breast
9.9
14.3



Breast Cancer
0.4
0.2



Breast Cancer (OD04590-01)
5.3
3.9



Breast Cancer Mets (OD04590-03)
4.0
10.4



Breast Cancer Metastasis
7.2
4.4



Breast Cancer
5.2
3.3



Breast Cancer
12.1
18.6



Breast Cancer 9100266
2.7
5.3



Breast Margin 9100265
5.0
5.8



Breast Cancer A209073
0.5
1.8



Breast Margin A209073
1.7
0.4



Normal Liver
39.5
47.0



Liver Cancer
4.2
0.6



Liver Cancer 1025
66.4
74.2



Liver Cancer 1026
36.1
42.6



Liver Cancer 6004-T
100.0
100.0



Liver Tissue 6004-N
22.8
34.4



Liver Cancer 6005-T
39.2
35.4



Liver Tissue 6005-N
33.2
38.2



Normal Bladder
6.6
4.9



Bladder Cancer
1.0
4.8



Bladder Cancer
2.6
0.7



Bladder Cancer (OD04718-01)
0.0
0.7



Bladder Normal Adjacent
3.5
14.4



(OD04718-03)



Normal Ovary
50.7
47.3



Ovarian Cancer
10.2
7.4



Ovarian Cancer (OD04768-07)
73.7
80.7



Ovary Margin (OD04768-08)
2.6
0.8



Normal Stomach
2.9
2.9



Gastric Cancer 9060358
0.0
1.1



Stomach Margin 9060359
2.4
0.3



Gastric Cancer 9060395
0.5
1.1



Stomach Margin 9060394
5.2
2.0



Gastric Cancer 9060397
3.4
7.0



Stomach Margin 9060396
1.4
0.0



Gastric Cancer 064005
1.3
6.0









Column A - Rel. Exp. (%) Ag746, Run 147127131






Column B - Rel. Exp. (%) Ag746, Run 148019631














TABLE BHH










Panel 4D











Tissue Name
A
B















Secondary Th1 act
10.9
7.7



Secondary Th2 act
6.4
8.0



Secondary Tr1 act
11.3
9.3



Secondary Th1 rest
3.4
2.7



Secondary Th2 rest
1.5
2.5



Secondary Tr1 rest
1.4
2.0



Primary Th1 act
48.0
46.0



Primary Th2 act
38.7
27.7



Primary Tr1 act
72.2
55.5



Primary Th1 rest
3.1
2.3



Primary Th2 rest
1.0
0.8



Primary Tr1 rest
1.1
0.5



CD45RA CD4 lymphocyte act
2.9
1.8



CD45RO CD4 lymphocyte act
18.6
12.2



CD8 lymphocyte act
17.8
6.8



Secondary CD8 lymphocyte rest
6.8
6.0



Secondary CD8 lymphocyte act
5.5
4.1



CD4 lymphocyte none
0.0
0.2



2ry Th1/Th2/Tr1 anti-CD95
2.9
3.1



CH11



LAK cells rest
1.4
0.3



LAK cells IL-2
3.8
2.2



LAK cells IL-2 + IL-12
3.0
0.8



LAK cells IL-2 + IFN gamma
2.0
1.7



LAK cells IL-2 + IL-18
0.5
0.2



LAK cells PMA/ionomycin
0.7
1.3



NK Cells IL-2 rest
0.7
0.7



Two Way MLR 3 day
1.1
2.5



Two Way MLR 5 day
2.5
2.8



Two Way MLR 7 day
4.5
5.0



PBMC rest
0.0
0.0



PBMC PWM
41.8
29.1



PBMC PHA-L
34.4
21.8



Ramos (B cell) none
4.7
2.4



Ramos (B cell) ionomycin
9.2
5.8



B lymphocytes PWM
51.8
51.4



B lymphocytes CD40L and IL-4
10.2
12.3



EOL-1 dbcAMP
0.3
0.2



EOL-1 dbcAMP
0.4
1.8



PMA/ionomycin



Dendritic cells none
6.7
3.8



Dendritic cells LPS
4.7
3.1



Dendritic cells anti-CD40
6.0
5.6



Monocytes rest
0.0
0.0



Monocytes LPS
0.7
0.8



Macrophages rest
19.8
9.9



Macrophages LPS
0.7
0.5



HUVEC none
9.3
10.2



HUVEC starved
19.2
13.1



HUVEC IL-1beta
4.1
1.7



HUVEC IFN gamma
21.0
13.7



HUVEC TNF alpha + IFN gamma
2.8
0.6



HUVEC TNF alpha + IL4
30.8
25.7



HUVEC IL-11
11.6
7.3



Lung Microvascular EC none
24.1
20.0



Lung Microvascular EC TNFalpha + IL-
8.0
12.2



1beta



Microvascular Dermal EC none
64.6
45.7



Microsvasular Dermal EC TNFalpha + IL-
18.4
11.7



1beta



Bronchial epithelium
5.2
5.4



TNFalpha + IL1beta



Small airway epithelium none
4.0
3.2



Small airway epithelium TNFalpha + IL-
8.2
4.5



1beta



Coronery artery SMC rest
5.8
6.3



Coronery artery SMC
4.5
5.1



TNFalpha + IL-1beta



Astrocytes rest
0.8
0.5



Astrocytes TNFalpha + IL-1beta
3.6
1.9



KU-812 (Basophil) rest
16.0
11.1



KU-812 (Basophil) PMA/ionomycin
12.3
9.5



CCD1106 (Keratinocytes) none
0.0
0.5



93580 CCD1106 (Keratinocytes) TNFa and
0.7
0.4



IFNg



Liver cirrhosis
8.4
3.8



Lupus kidney
2.0
3.2



NCI-H292 none
21.9
25.7



NCI-H292 IL-4
15.7
12.3



NCI-H292 IL-9
20.6
14.7



NCI-H292 IL-13
8.3
5.7



NCI-H292 IFN gamma
5.1
8.2



HPAEC none
18.7
23.8



HPAEC TNF alpha + IL-1 beta
11.9
12.9



Lung fibroblast none
15.7
13.5



Lung fibroblast TNF alpha + IL-1 beta
6.9
4.7



Lung fibroblast IL-4
25.0
16.6



Lung fibroblast IL-9
14.7
15.8



Lung fibroblast IL-13
40.3
32.5



Lung fibroblast IFN gamma
15.4
17.4



Dermal fibroblast CCD1070 rest
0.5
0.9



Dermal fibroblast CCD1070 TNF alpha
0.9
0.8



Dermal fibroblast CCD1070 IL-1 beta
0.6
0.6



Dermal fibroblast IFN gamma
32.1
18.4



Dermal fibroblast IL-4
100.0
100.0



IBD Colitis 2
0.0
0.0



IBD Crohn's
0.3
0.8



Colon
1.4
0.5



Lung
0.5
0.8



Thymus
2.9
4.3



Kidney
65.5
47.3









Column A - Rel. Exp. (%) Ag1294b, Run 138944262






Column B - Rel. Exp. (%) Ag1294b, Run 139408252







AI_comprehensive panel_v1.0 Summary: Ag1294b Expression of this gene in this panel confirmed expression of this gene in cells involved in the immune response. Highest expression of this gene was seen in normal lung (CT=30.5). Please see Panel 4D for discussion of utility of this gene in inflammation.


General_screeningpanel_v1.4 Summary: Ag4726 Highest expression of this gene was seen in a liver cancer cell line (CTs=30), with moderate levels of expression seen in fetal and adult liver, and cell lines derived from colon, renal and lung cancers. Thus, expression of this gene is useful as a marker to differentiate liver derived tissue from other samples on this panel.


Panel 1.2 Summary: Ag746 Highest expression of this gene was detected in a liver cancer cell line (CTs=27). High levels of expression were also seen in fetal and adult liver tissue, a colon cancer cell line and a lung cancer cell line. Thus, expression of this gene is useful as a marker to differentiate liver derived samples, the colon cancer cell line and the lung cancer cell line from other samples on this panel. Expression of this gene is also useful as a diagnostic marker to detect the presence of colon and lung cancers.


Moderate expression was also seen in the fetal brain, placenta, and endothelial cells.


Panel 2D Summary: Ag746 Highest expression of this gene was detected in a liver cancer sample (CTs=31). The prominent expression in liver derived tissue was consistent with the results in Panel 1.2. Moderate levels of expression were also evident in samples from ovarian cancer and kidney cancer. Expression of this gene was higher in these cancers than in the normal adjacent tissue. Thus, expression of this gene is useful as a marker to differentiate between liver derived samples and other samples on this panel and as a marker to detect the presence of liver, kidney, and ovarian cancer. Therapeutic modulation of the expression or function of this gene or its protein product is useful in the treatment of liver, kidney, and ovarian cancers.


Panel 4D Summary: Ag1294b Highest expression of this gene was detected in IL-4 treated dermal fibroblasts (CTs=30). In addition, this gene was expressed at moderate levels in IFN gamma stimulated dermal fibroblasts, activated lung fibroblasts, HPAECs, lung and dermal microvasculature, activated small airway and bronchial epithelium, activated NCI-H292 cells, acutely activated T cells, and activated B cells.


Based on these levels of expression in T cells, activated B cells and cells in lung and skin, therapeutics that block the function of this gene product are useful as therapeutics that reduce or eliminate the symptoms in patients with autoimmune and inflammatory diseases in which activated B cells present antigens in the generation of the aberrant immune response and in treating T-cell mediated diseases, including Crohn's disease, ulcerative colitis, multiple sclerosis, chronic obstructive pulmonary disease, asthma, allergy, emphysema, rheumatoid arthritis, or psoriasis.


BE. CG94946-01: Agrin Precursor


Expression of gene CG94946-01 was assessed using the primer-probe sets Ag3605 and Ag3974, described in Tables BIA and BIB. Results of the RTQ-PCR runs are shown in Tables BIC, BID, BIE and BIF.

TABLE BIAProbe Name Ag36O5+HL,6SEQStartIDPrimersSequencesLengthPositionNoForward5′-gaccccaagtcagaactgttc-3′2135141591ProbeTET-5′-attgagagcaccctggacgacctctt-3′-2635531592TAMRAReverse5′-gaaatccttcttgacgtctgaa-3′2235851593









TABLE BIB










Probe Name Ag3974













+HL,6


SEQ






Start
ID


Primers
Sequences
Length
Position
No





Forward
5′-gacaccaggatcttctttgtga-3′
22
379
1594






Probe
TET-5′-catacctgtggccagcccacaag-3′-TAMRA
23
413
1595





Reverse
5′-gagttgagcatcagctcgtt-3′
20
436
1596
















TABLE BIC










General_screening_panel_v1.4











Tissue Name
A
B















Adipose
1.4
1.5



Melanoma* Hs688(A).T
2.6
3.2



Melanoma* Hs688(B).T
4.6
4.2



Melanoma* M14
6.7
6.4



Melanoma* LOXIMVI
4.8
4.0



Melanoma* SK-MEL-5
2.6
4.2



Squamous cell carcinoma SCC-4
9.0
8.4



Testis Pool
1.3
1.1



Prostate ca.* (bone met) PC-3
21.0
24.8



Prostate Pool
0.9
0.8



Placenta
0.9
1.3



Uterus Pool
0.4
0.4



Ovarian ca. OVCAR-3
77.4
66.9



Ovarian ca. SK-OV-3
42.0
36.3



Ovarian ca. OVCAR-4
9.5
12.7



Ovarian ca. OVCAR-5
39.2
44.4



Ovarian ca. IGROV-1
22.1
27.7



Ovarian ca. OVCAR-8
18.0
14.9



Ovary
1.5
1.9



Breast ca. MCF-7
7.7
9.7



Breast ca. MDA-MB-231
22.7
31.2



Breast ca. BT 549
13.2
10.1



Breast ca. T47D
100.0
100.0



Breast ca. MDA-N
4.8
4.2



Breast Pool
1.6
1.6



Trachea
2.8
2.6



Lung
0.2
0.1



Fetal Lung
11.4
8.3



Lung ca. NCI-N417
1.4
0.7



Lung ca. LX-1
10.5
11.0



Lung ca. NCI-H146
0.1
0.1



Lung ca. SHP-77
1.1
0.8



Lung ca. A549
15.6
10.4



Lung ca. NCI-H526
5.4
1.6



Lung ca. NCI-H23
18.9
20.6



Lung ca. NCI-H460
11.5
9.3



Lung ca. HOP-62
23.7
23.0



Lung ca. NCI-H522
1.8
2.3



Liver
0.5
0.6



Fetal Liver
0.8
1.4



Liver ca. HepG2
15.5
12.6



Kidney Pool
1.5
2.5



Fetal Kidney
5.8
4.6



Renal ca. 786-0
46.3
39.5



Renal ca. A498
13.8
7.9



Renal ca. ACHN
14.3
15.9



Renal ca. UO-31
41.5
38.7



Renal ca. TK-10
19.3
16.4



Bladder
8.4
9.0



Gastric ca. (liver met.) NCI-N87
87.7
80.7



Gastric ca. KATO III
17.8
17.7



Colon ca. SW-948
9.2
7.8



Colon ca. SW480
25.0
32.3



Colon ca.* (SW480 met) SW620
5.0
4.6



Colon ca. HT29
26.8
30.6



Colon ca. HCT-116
4.9
5.8



Colon ca. CaCo-2
13.6
10.4



Colon cancer tissue
10.2
10.0



Colon ca. SW1116
5.1
3.6



Colon ca. Colo-205
1.8
1.5



Colon ca. SW-48
1.2
0.7



Colon Pool
1.9
1.3



Small Intestine Pool
0.6
1.0



Stomach Pool
1.5
1.2



Bone Marrow Pool
0.6
0.5



Fetal Heart
1.4
1.0



Heart Pool
0.7
0.8



Lymph Node Pool
2.1
2.0



Fetal Skeletal Muscle
0.9
0.5



Skeletal Muscle Pool
0.4
0.5



Spleen Pool
0.7
0.7



Thymus Pool
1.8
2.2



CNS cancer (glio/astro) U87-MG
5.9
6.0



CNS cancer (glio/astro) U-118-MG
11.7
11.2



CNS cancer (neuro; met) SK-N-AS
1.2
0.9



CNS cancer (astro) SF-539
6.7
5.0



CNS cancer (astro) SNB-75
22.8
32.3



CNS cancer (glio) SNB-19
25.0
20.2



CNS cancer (glio) SF-295
35.1
38.2



Brain (Amygdala) Pool
1.7
1.3



Brain (cerebellum)
1.4
1.0



Brain (fetal)
7.0
2.8



Brain (Hippocampus) Pool
1.6
0.9



Cerebral Cortex Pool
1.9
0.9



Brain (Substantia nigra) Pool
2.8
1.7



Brain (Thalamus) Pool
2.7
1.6



Brain (whole)
3.4
1.1



Spinal Cord Pool
1.8
1.4



Adrenal Gland
0.2
0.4



Pituitary gland Pool
0.3
0.2



Salivary Gland
1.1
1.3



Thyroid (female)
3.3
3.7



Pancreatic ca. CAPAN2
23.7
27.7



Pancreas Pool
3.0
4.1









Column A - Rel. Exp. (%) Ag3605, Run 213406184






Column B - Rel. Exp. (%) Ag3974, Run 217508632














TABLE BID










Panel 2.2










Tissue Name
A














Normal Colon
4.7



Colon cancer (OD06064)
6.3



Colon Margin (OD06064)
2.3



Colon cancer (OD06159)
2.1



Colon Margin (OD06159)
1.8



Colon cancer (OD06297-04)
2.0



Colon Margin (OD06297-05)
3.0



CC Gr.2 ascend colon (ODO3921)
3.6



CC Margin (ODO3921)
1.3



Colon cancer metastasis (OD06104)
1.1



Lung Margin (OD06104)
1.0



Colon mets to lung (OD04451-01)
4.5



Lung Margin (OD04451-02)
6.7



Normal Prostate
2.1



Prostate Cancer (OD04410)
3.9



Prostate Margin (OD04410)
3.4



Normal Ovary
7.6



Ovarian cancer (OD06283-03)
27.9



Ovarian Margin (OD06283-07)
2.0



Ovarian Cancer
16.0



Ovarian cancer (OD06145)
10.1



Ovarian Margin (OD06145)
8.2



Ovarian cancer (OD06455-03)
28.9



Ovarian Margin (OD06455-07)
1.9



Normal Lung
2.9



Invasive poor diff. lung adeno (ODO4945-01
9.4



Lung Margin (ODO4945-03)
7.9



Lung Malignant Cancer (OD03126)
7.5



Lung Margin (OD03126)
7.0



Lung Cancer (OD05014A)
17.0



Lung Margin (OD05014B)
11.7



Lung cancer (OD06081)
12.2



Lung Margin (OD06081)
2.4



Lung Cancer (OD04237-01)
1.8



Lung Margin (OD04237-02)
16.2



Ocular Mel Met to Liver (ODO4310)
8.4



Liver Margin (ODO4310)
2.9



Melanoma Metastasis
4.0



Lung Margin (OD04321)
3.8



Normal Kidney
10.9



Kidney Ca, Nuclear grade 2 (OD04338)
35.4



Kidney Margin (OD04338)
20.4



Kidney Ca Nuclear grade 1/2 (OD04339)
52.9



Kidney Margin (OD04339)
16.6



Kidney Ca, Clear cell type (OD04340)
16.6



Kidney Margin (OD04340)
7.4



Kidney Ca, Nuclear grade 3 (OD04348)
11.2



Kidney Margin (OD04348)
100.0



Kidney malignant cancer (OD06204B)
21.6



Kidney normal adjacent tissue (OD06204E)
23.2



Kidney Cancer (OD04450-01)
53.2



Kidney Margin (OD04450-03)
21.3



Kidney Cancer 8120613
1.1



Kidney Margin 8120614
14.1



Kidney Cancer 9010320
20.3



Kidney Margin 9010321
15.0



Kidney Cancer 8120607
71.7



Kidney Margin 8120608
12.2



Normal Uterus
7.3



Uterine Cancer 064011
6.9



Normal Thyroid
4.3



Thyroid Cancer
27.0



Thyroid Cancer A302152
19.1



Thyroid Margin A302153
8.1



Normal Breast
14.0



Breast Cancer
13.4



Breast Cancer
35.1



Breast Cancer (OD04590-01)
31.6



Breast Cancer Mets (OD04590-03)
8.7



Breast Cancer Metastasis
13.3



Breast Cancer
21.5



Breast Cancer 9100266
17.7



Breast Margin 9100265
16.5



Breast Cancer A209073
13.2



Breast Margin A209073
35.4



Breast cancer (OD06083)
24.5



Breast cancer node metastasis (OD06083)
21.5



Normal Liver
5.0



Liver Cancer 1026
15.5



Liver Cancer 1025
12.2



Liver Cancer 6004-T
7.8



Liver Tissue 6004-N
6.1



Liver Cancer 6005-T
25.0



Liver Tissue 6005-N
12.4



Liver Cancer
12.9



Normal Bladder
14.2



Bladder Cancer
9.5



Bladder Cancer
12.2



Normal Stomach
8.7



Gastric Cancer 9060397
8.9



Stomach Margin 9060396
7.4



Gastric Cancer 9060395
7.0



Stomach Margin 9060394
7.5



Gastric Cancer 064005
6.9









Column A - Rel. Exp. (%) Ag3605, Run 173764229














TABLE BIE










Panel 4.1D











Tissue Name
A
B















Secondary Th1 act
1.0
1.2



Secondary Th2 act
5.1
8.0



Secondary Tr1 act
2.5
3.5



Secondary Th1 rest
0.0
0.7



Secondary Th2 rest
0.4
0.2



Secondary Tr1 rest
0.4
1.2



Primary Th1 act
3.6
3.2



Primary Th2 act
1.1
2.0



Primary Tr1 act
3.4
2.9



Primary Th1 rest
0.9
0.4



Primary Th2 rest
0.5
0.2



Primary Tr1 rest
0.2
0.3



CD45RA CD4 lymphocyte act
43.5
22.7



CD45RO CD4 lymphocyte act
5.0
5.5



CD8 lymphocyte act
3.9
3.3



Secondary CD8 lymphocyte rest
3.7
3.3



Secondary CD8 lymphocyte act
3.3
3.5



CD4 lymphocyte none
0.3
0.1



2ry Th1/Th2/Tr1 anti-CD95 CH11
0.3
0.4



LAK cells rest
5.0
6.4



LAK cells IL-2
2.8
1.7



LAK cells IL-2 + IL-12
1.4
1.8



LAK cells IL-2 + IFN gamma
2.3
1.1



LAK cells IL-2 + IL-18
2.9
1.6



LAK cells PMA/ionomycin
6.8
4.6



NK Cells IL-2 rest
1.6
1.9



Two Way MLR 3 day
10.7
12.4



Two Way MLR 5 day
6.5
5.3



Two Way MLR 7 day
4.3
4.0



PBMC rest
0.0
0.6



PBMC PWM
5.0
4.9



PBMC PHA-L
5.5
3.4



Ramos (B cell) none
0.4
0.4



Ramos (B cell) ionomycin
0.2
0.2



B lymphocytes PWM
2.4
3.0



B lymphocytes CD40L and IL-4
1.5
3.7



EOL-1 dbcAMP
3.4
3.1



EOL-1 dbcAMP PMA/ionomycin
18.3
8.0



Dendritic cells none
14.8
9.0



Dendritic cells LPS
48.3
32.8



Dendritic cells anti-CD40
9.7
8.8



Monocytes rest
0.9
1.4



Monocytes LPS
66.4
81.2



Macrophages rest
16.2
9.7



Macrophages LPS
54.7
43.8



HUVEC none
9.3
12.6



HUVEC starved
14.4
25.2



HUVEC IL-1beta
15.6
18.9



HUVEC IFN gamma
12.9
16.7



HUVEC TNF alpha + IFN gamma
37.6
34.9



HUVEC TNF alpha + IL4
31.4
31.4



HUVEC IL-11
14.9
13.9



Lung Microvascular EC none
79.0
100.0



Lung Microvascular EC TNFalpha + IL-
100.0
97.9



1beta



Microvascular Dermal EC none
49.7
48.3



Microsvasular Dermal EC TNFalpha + IL-
56.6
47.0



1beta



Bronchial epithelium TNFalpha + IL1beta
78.5
90.1



Small airway epithelium none
31.0
32.5



Small airway epithelium TNFalpha + IL-
81.8
93.3



1beta



Coronery artery SMC rest
17.2
28.5



Coronery artery SMC TNFalpha + IL-1beta
22.2
28.7



Astrocytes rest
80.1
55.1



Astrocytes TNFalpha + IL-1beta
82.9
66.4



KU-812 (Basophil) rest
2.6
1.9



KU-812 (Basophil) PMA/ionomycin
0.6
2.8



CCD1106 (Keratinocytes) none
70.7
82.4



CCD1106 (Keratinocytes) TNFalpha + IL-
77.4
72.7



1beta



Liver cirrhosis
13.2
14.4



NCI-H292 none
57.8
54.0



NCI-H292 IL-4
62.4
78.5



NCI-H292 IL-9
61.6
79.6



NCI-H292 IL-13
53.6
59.9



NCI-H292 IFN gamma
67.4
71.7



HPAEC none
21.5
21.3



HPAEC TNF alpha + IL-1 beta
37.6
45.4



Lung fibroblast none
22.4
29.3



Lung fibroblast TNF alpha + IL-1 beta
71.2
87.7



Lung fibroblast IL-4
16.2
23.3



Lung fibroblast IL-9
31.9
30.4



Lung fibroblast IL-13
18.7
36.6



Lung fibroblast IFN gamma
23.0
29.7



Dermal fibroblast CCD1070 rest
15.9
27.2



Dermal fibroblast CCD1070 TNF alpha
15.9
20.6



Dermal fibroblast CCD1070 IL-1 beta
17.2
22.4



Dermal fibroblast IFN gamma
7.2
10.3



Dermal fibroblast IL-4
7.2
8.0



Dermal Fibroblasts rest
4.3
6.3



Neutrophils TNFa + LPS
0.0
0.9



Neutrophils rest
0.2
1.0



Colon
7.0
5.8



Lung
23.3
23.3



Thymus
5.8
7.3



Kidney
23.2
33.2









Column A - Rel. Exp. (%) Ag3605, Run 169943454






Column B - Rel. Exp. (%) Ag3974, Run 170739806














TABLE BIF










general oncology screening panel_v_2.4










Tissue Name
A














Colon cancer 1
11.6



CC Margin (ODO3921)
5.3



Colon cancer 2
18.6



Colon NAT 2
3.2



Colon cancer 3
22.2



Colon NAT 3
7.7



Colon malignant cancer 4
23.5



Colon NAT 4
2.0



Lung cancer 1
57.0



Lung NAT 1
3.1



Lung cancer 2
100.0



Lung NAT 2
3.9



Squamous cell carcinoma 3
41.2



Lung NAT 3
1.7



Metastatic melanoma 1
5.4



Melanoma 2
3.2



Melanoma 3
3.7



Metastatic melanoma 4
12.5



Metastatic melanoma 5
7.9



Bladder cancer 1
0.5



Bladder NAT 1
0.0



Bladder cancer 2
6.0



Bladder NAT 2
0.2



Bladder NAT 3
1.7



Bladder NAT 4
1.8



Prostate adenocarcinoma 1
2.4



Prostate adenocarcinoma 2
2.6



Prostate adenocarcinoma 3
5.2



Prostate adenocarcinoma 4
21.9



Prostate NAT 5
5.4



Prostate adenocarcinoma 6
4.4



Prostate adenocarcinoma 7
3.8



Prostate adenocarcinoma 8
1.8



Prostate adenocarcinoma 9
10.7



Prostate NAT 10
0.7



Kidney cancer 1
38.7



Kidney NAT 1
20.6



Kidney cancer 2
66.4



Kidney NAT 2
39.8



Kidney cancer 3
64.6



Kidney NAT 3
19.6



Kidney cancer 4
16.4



Kidney NAT 4
15.6









Column A - Rel. Exp. (%) Ag3605, Run 260268655







General_screening_panel_v1.4 Summary: Ag3605/Ag3974 The expression of this gene was highest in a sample derived from a breast cancer cell line (T47D) (CTs=22.5-25.3). In addition, there was substantial expression in other samples derived from breast cancer cell lines, ovarian cancer cell lines, kidney cancer cell lines, lung cancer cell lines, colon cancer cell lines and brain cancer cell lines. Thus, the expression of this gene is useful as a marker to distinguish T47D cells from other samples in the panel. Therapeutic modulation of this gene or its protein product through the use of small molecule drugs, protein therapeutics, or antibodies is of benefit in the treatment of breast, ovarian, kidney, lung, colon or brain cancer.


Among metabolic tissues, this gene had low-to-moderate levels of expression in adrenal, pituitary, adult and fetal heart, adult and fetal liver, adult and fetal skeletal muscle, and adipose. This gene product had high levels of expression (CT values=27) in pancreas and thyroid. Thus, this gene product is important for the pathogenesis, diagnosis, and/or treatment of metabolic and endocrine diseases, including obesity, Types 1 and 2 diabetes and thyroidopathies. Decreased glomerular expression of agrin has been observed in diabetic nephropathy and supports this use of this gene or gene product. (Yard B A, Exp Nephrol 2001;9(3):214-22 ).


In addition, this gene was expressed at moderate levels in all regions of the central nervous system examined, including amygdala, hippocampus, substantia nigra, thalamus, cerebellum, cerebral cortex, and spinal cord. This gene encodes a protein with homology to agrin, a neuronal aggregating factor that induces the aggregation of acetylcholine receptors and other postsynaptic proteins on muscle fibers and is crucial for the formation of the neuromuscular junction. Agrin also plays an important role in defining neuronal responses to excitatory neurotransmitters both in vitro and in vivo (Hilgenberg L G, Mol Cell Neurosci 2002 January; 19(1):97-110 and Bixby J L, J Neurobiol 2002 February 5;50(2):164-79). The gene expression in the central nervous system is consistent the use of the protein and its function as an agrin. This gene plays a role in central nervous system disorders such as Alzheimer's disease, Parkinson's disease, epilepsy, multiple sclerosis, schizophrenia and depression.


Agrin has also been implicated in the formation of senile plaques in Alzheimer's disease and in the acetylcholine synapse/neuromuscular junction (van Horssen J, Acta Neuropathol (Berl) 2001 December;102(6):604-14). In addition, an agrin minigene rescued dystrophic symptoms in a mouse model of muscular dystrophy (Moll J, Nature. 2001 September 20;413(6853):302-7). Therefore, this gene or gene product is useful as a treatment or cure for congenital muscular dystrophies. Furthermore, this gene product is also an excellent drug target in AD or in any disease involving the neuromuscular junction or the acetylcholine system.


Panel 2.2 Summary: Ag3605 Expression of this gene was highest in a sample of normal kidney (CT=27.4). In addition, expression of this gene was upregulated in a number of ovarian and renal cancers when compared to the matched control margins.


Thus, expression of this gene is useful as a marker for ovarian and renal carcinoma. Furthermore, therapeutic modulation of the activity of this gene or its protein product, using small molecule drugs, antibodies or protein therapeutics, is of benefit in the treatment of renal and ovarian cancer.


Panel 4.1D Summary: Ag3605/Ag3974 Highest expression of this gene was highest in lung microvascular endothelial cells (CTs=27.3-28.5), microvascular dermal endothelial cells, mucoepidermoid cell line NCI-H292, astrocytes, and keratinocytes.


This gene encodes a protein with homology to agrin. Agrin, an aggregating protein crucial for formation of the neuromuscular junction, is important for T cell signaling in the immune system (Khan AA, Science 2001 June 1;292(5522):1681-6). In addition, agrin has been identified as a potential disease target for autoimmune disorders at the neuromuscularjunction, including multiple sclerosis (Liyanage Y, Muscle Nerve 2002 January;25(1):4-16). Therefore, small molecule drug, antibody or protein therapeutics that target this gene or the protein encoded by the gene is useful for the reduction or inhibition of inflammation in asthma, emphysema, allergy, psoriasis, muscular dystrophy and multiple sclerosis.


general oncology screening panel_V—2.4 Summary: Ag3605 Highest expression of this gene was seen in a lung cancer sample (CT=26.8). In addition, higher levels of expression were seen in lung and kidney cancers when compared to expression in normal adjacent tissue. Thus, expression of this gene is useful as a marker of lung and kidney cancers. Therapeutic modulation of the expression or function of this gene or gene product is useful in the treatment of lung and kidney cancers.


BJ. CG9638401: Novel Plasma Membrane Protein


Expression of gene CG96384-01 was assessed using the primer-probe set Ag4093, described in Table BJA. Results of the RTQ-PCR run are shown in Table BJB.

TABLE BJAProbe Name Ag4093+HL,6SEQStartIDPrimersSequencesLengthPositionNoForward5′-agctggagcacaggagagat-3′201581597ProbeTET-5′-ggaagctctcctttgacactcgttcc-3′-261971598TAMRAReverse5′-acccatggtcttccagaaag-3′202311599









TABLE BJB










Panel 4.1D










Tissue Name
A














Secondary Th1 act
55.1



Secondary Th2 act
87.7



Secondary Tr1 act
82.9



Secondary Th1 rest
30.4



Secondary Th2 rest
43.2



Secondary Tr1 rest
38.7



Primary Th1 act
57.4



Primary Th2 act
100.0



Primary Tr1 act
62.0



Primary Th1 rest
28.3



Primary Th2 rest
21.5



Primary Tr1 rest
42.0



CD45RA CD4 lymphocyte act
40.9



CD45RO CD4 lymphocyte act
84.1



CD8 lymphocyte act
70.2



Secondary CD8 lymphocyte rest
56.6



Secondary CD8 lymphocyte act
26.6



CD4 lymphocyte none
36.1



2ry Th1/Th2/Tr1 anti-CD95 CH11
67.4



LAK cells rest
38.2



LAK cells IL-2
46.0



LAK cells IL-2 + IL-12
42.3



LAK cells IL-2 + IFN gamma
25.7



LAK cells IL-2 + IL-18
35.4



LAK cells PMA/ionomycin
47.0



NK Cells IL-2 rest
74.7



Two Way MLR 3 day
36.3



Two Way MLR 5 day
19.6



Two Way MLR 7 day
58.6



PBMC rest
26.1



PBMC PWM
10.9



PBMC PHA-L
42.9



Ramos (B cell) none
18.9



Ramos (B cell) ionomycin
6.1



B lymphocytes PWM
27.7



B lymphocytes CD40L and IL-4
48.0



EOL-1 dbcAMP
21.2



EOL-1 dbcAMP PMA/ionomycin
31.4



Dendritic cells none
17.4



Dendritic cells LPS
10.7



Dendritic cells anti-CD40
16.7



Monocytes rest
29.5



Monocytes LPS
28.3



Macrophages rest
13.9



Macrophages LPS
7.6



HUVEC none
14.8



HUVEC starved
36.6



HUVEC IL-1beta
18.8



HUVEC IFN gamma
23.3



HUVEC TNF alpha + IFN gamma
11.3



HUVEC TNF alpha + IL4
20.7



HUVEC IL-11
12.2



Lung Microvascular EC none
21.0



Lung Microvascular EC TNFalpha + IL-1beta
15.3



Microvascular Dermal EC none
12.6



Microsvasular Dermal EC TNFalpha + IL-1beta
15.8



Bronchial epithelium TNFalpha + IL1beta
16.8



Small airway epithelium none
9.3



Small airway epithelium TNFalpha + IL-1beta
14.2



Coronery artery SMC rest
8.9



Coronery artery SMC TNFalpha + IL-1 beta
7.3



Astrocytes rest
9.8



Astrocytes TNFalpha + IL-1beta
8.2



KU-812 (Basophil) rest
11.4



KU-812 (Basophil) PMA/ionomycin
18.3



CCD1106 (Keratinocytes) none
13.2



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
17.3



Liver cirrhosis
9.7



NCI-H292 none
15.8



NCI-H292 IL-4
32.5



NCI-H292 IL-9
33.2



NCI-H292 IL-13
45.1



NCI-H292 IFN gamma
12.8



HPAEC none
24.3



HPAEC TNF alpha + IL-1 beta
36.6



Lung fibroblast none
18.7



Lung fibroblast TNF alpha + IL-1 beta
2.9



Lung fibroblast IL-4
6.4



Lung fibroblast IL-9
17.4



Lung fibroblast IL-13
15.5



Lung fibroblast IFN gamma
14.9



Dermal fibroblast CCD1070 rest
17.9



Dermal fibroblast CCD1070 TNF alpha
67.8



Dermal fibroblast CCD1070 IL-1 beta
15.2



Dermal fibroblast IFN gamma
9.7



Dermal fibroblast IL-4
44.1



Dermal Fibroblasts rest
16.6



Neutrophils TNFa + LPS
2.4



Neutrophils rest
19.6



Colon
8.1



Lung
8.7



Thymus
84.1



Kidney
55.9









Column A - Rel. Exp. (%) Ag4093, Run 172383903







Panel 4.1D Summary: Ag4093 This gene was widely expressed in this panel, with highest expression in primary activated Th2 cells (CT=32.7). This gene was also expressed. at high to moderate levels in a wide range of cell types of significance in the immune response in health and disease. These cells include members of the T-cell, B-cell, endothelial cell, monocyte, and peripheral blood mononuclear cell families, as well as dermal fibroblasts and normal tissues represented from thymus and kidney. This pattern of expression shows that this gene product is involved in homeostatic processes for these and other cell types and tissues. Therefore, modulation of the gene or gene product with a functional therapeutic is useful in the alteration of functions associated with these cell types and in the treatment of the symptoms of patients suffering from autoimmune and inflammatory diseases such as asthma, allergies, inflammatory bowel disease, lupus erythematosus, psoriasis, rheumatoid arthritis, and osteoarthritis.


BK. CG98011-01: Insulin-like Growth Factor Binding Protein


Expression of gene CG9801 1-01 was assessed using the primer-probe set Ag2120, described in Table BKA. Results of the RTQ-PCR runs are shown in Tables BKB, BKC, BKD and BKE.

TABLE BKAProbe Name Ag2120+HL,6SEQStartIDPrimersSequencesLengthPositionNoForward5′-gctgattgcaagaagatgtttc-3′221031600ProbeTET-5′-ttttgtcagccctgatttcttcgaca-3′-261401601Reverse5′-ccgatatgtcagaatctgcatt-3′221661602









TABLE BKB










Panel 1.3D











Tissue Name
A
B















Liver adenocarcinoma
0.5
0.1



Pancreas
0.3
0.2



Pancreatic ca. CAPAN 2
1.0
1.1



Adrenal gland
12.8
10.8



Thyroid
2.1
1.3



Salivary gland
0.6
1.1



Pituitary gland
0.6
0.3



Brain (fetal)
7.9
8.8



Brain (whole)
26.8
23.8



Brain (amygdala)
27.5
17.8



Brain (cerebellum)
17.4
19.2



Brain (hippocampus)
64.6
58.6



Brain (substantia nigra)
2.2
3.5



Brain (thalamus)
3.6
3.0



Cerebral Cortex
100.0
100.0



Spinal cord
0.5
1.2



glio/astro U87-MG
0.8
0.5



glio/astro U-118-MG
3.2
4.7



astrocytoma SW1783
0.7
0.8



neuro*; met SK-N-AS
0.4
0.2



astrocytoma SF-539
0.4
0.3



astrocytoma SNB-75
0.5
0.2



glioma SNB-19
0.4
1.2



glioma U251
1.6
1.3



glioma SF-295
1.9
0.8



Heart (Fetal)
3.3
2.2



Heart
0.3
0.6



Skeletal muscle (Fetal)
6.8
9.3



Skeletal muscle
3.4
4.0



Bone marrow
0.9
1.6



Thymus
0.9
0.5



Spleen
0.4
0.1



Lymph node
1.2
0.6



Colorectal
1.6
1.1



Stomach
1.0
0.2



Small intestine
0.2
2.0



Colon ca. SW480
0.8
1.6



Colon ca.* SW620 (SW480 met)
0.0
1.4



Colon ca. HT29
0.2
0.5



Colon ca. HCT-116
0.3
0.2



Colon ca. CaCo-2
0.6
0.0



CC Well to Mod Diff (ODO3866)
1.5
0.5



Colon ca. HCC-2998
0.6
0.3



Gastric ca. (liver met) NCI-N87
0.5
1.2



Bladder
1.8
1.0



Trachea
0.6
1.3



Kidney
1.3
2.5



Kidney (fetal)
1.7
4.0



Renal ca. 786-0
3.8
3.7



Renal ca. A498
2.6
3.0



Renal ca. RXF 393
2.2
2.3



Renal ca. ACHN
1.1
0.8



Renal ca. UO-31
0.5
1.2



Renal ca. TK-10
3.7
3.6



Liver
0.8
0.0



Liver (fetal)
0.2
0.1



Liver ca. (hepatoblast) HepG2
0.7
0.8



Lung
0.2
0.7



Lung (fetal)
0.4
0.6



Lung ca. (small cell) LX-1
1.2
0.1



Lung ca. (small cell) NCI-H69
44.8
40.9



Lung ca. (s. cell var.) SHP-77
5.6
5.2



Lung ca. (large cell)NCI-H460
54.3
74.2



Lung ca. (non-sm. cell) A549
0.5
0.0



Lung ca. (non-s. cell) NCI-H23
1.5
2.0



Lung ca. (non-s. cell) HOP-62
7.0
5.3



Lung ca. (non-s. cl) NCI-H522
1.1
0.0



Lung ca. (squam.) SW 900
0.3
0.8



Lung ca. (squam.) NCI-H596
8.4
11.1



Mammary gland
2.4
2.0



Breast ca.* (pl. ef) MCF-7
1.0
0.7



Breast ca.* (pl. ef) MDA-MB-231
0.0
0.0



Breast ca.* (pl. ef) T47D
17.3
18.7



Breast ca. BT-549
4.2
4.7



Breast ca. MDA-N
9.3
9.9



Ovary
12.4
9.9



Ovarian ca. OVCAR-3
0.1
0.0



Ovarian ca. OVCAR-4
0.5
2.7



Ovarian ca. OVCAR-5
0.9
0.9



Ovarian ca. OVCAR-8
2.1
4.0



Ovarian ca. IGROV-1
0.4
0.3



Ovarian ca. (ascites) SK-OV-3
0.8
0.1



Uterus
1.2
0.7



Placenta
1.8
0.1



Prostate
0.5
0.6



Prostate ca.* (bone met) PC-3
3.1
2.2



Testis
1.2
1.3



Melanoma Hs688(A).T
0.8
0.9



Melanoma* (met) Hs688(B).T
1.2
2.7



Melanoma UACC-62
0.7
0.7



Melanoma M14
4.0
5.5



Melanoma LOX IMVI
1.6
0.3



Melanoma* (met) SK-MEL-5
4.2
3.7



Adipose
3.1
1.9









Column A - Rel. Exp. (%) Ag2120, Run 149975625






Column B - Rel. Exp. (%) Ag2120, Run 150253839














TABLE BKC










Panel 2D











Tissue Name
A
B















Normal Colon
7.3
7.4



CC Well to Mod Diff (ODO3866)
4.3
7.9



CC Margin (ODO3866)
1.7
2.9



CC Gr.2 rectosigmoid (ODO3868)
2.3
1.5



CC Margin (ODO3868)
1.7
3.8



CC Mod Diff (ODO3920)
0.2
0.9



CC Margin (ODO3920)
0.1
2.5



CC Gr.2 ascend colon (ODO3921)
0.5
0.4



CC Margin (ODO3921)
5.8
6.4



CC from Partial Hepatectomy
6.1
8.7



(ODO4309) Mets



Liver Margin (ODO4309)
4.0
2.2



Colon mets to lung (OD04451-01)
0.0
0.3



Lung Margin (OD04451-02)
2.9
3.9



Normal Prostate 6546-1
3.0
7.8



Prostate Cancer (OD04410)
8.5
9.0



Prostate Margin (OD04410)
23.5
21.5



Prostate Cancer (OD04720-01)
7.3
7.1



Prostate Margin (OD04720-02)
1.7
11.3



Normal Lung
9.2
7.2



Lung Met to Muscle (ODO4286)
0.7
0.7



Muscle Margin (ODO4286)
11.3
12.5



Lung Malignant Cancer (OD03126)
7.9
4.7



Lung Margin (OD03126)
6.4
7.5



Lung Cancer (OD04404)
3.0
3.8



Lung Margin (OD04404)
2.6
2.8



Lung Cancer (OD04565)
1.8
3.4



Lung Margin (OD04565)
3.6
2.8



Lung Cancer (OD04237-01)
22.5
17.3



Lung Margin (OD04237-02)
3.5
4.5



Ocular Mel Met to Liver (ODO4310)
2.1
4.1



Liver Margin (ODO4310)
1.2
1.2



Melanoma Metastasis
2.5
1.7



Lung Margin (OD04321)
5.3
3.3



Normal Kidney
93.3
100.0



Kidney Ca, Nuclear grade 2 (OD04338)
55.9
92.7



Kidney Margin (OD04338)
37.9
36.6



Kidney Ca Nuclear grade 1/2 (OD04339)
67.4
76.3



Kidney Margin (OD04339)
25.3
33.2



Kidney Ca, Clear cell type (OD04340)
52.5
43.5



Kidney Margin (OD04340)
0.0
35.6



Kidney Ca, Nuclear grade 3 (OD04348)
5.0
7.6



Kidney Margin (OD04348)
20.7
26.6



Kidney Cancer (OD04622-01)
9.3
7.3



Kidney Margin (OD04622-03)
7.1
10.2



Kidney Cancer (OD04450-01)
27.0
29.3



Kidney Margin (OD04450-03)
34.2
33.7



Kidney Cancer 8120607
4.1
3.3



Kidney Margin 8120608
9.2
12.8



Kidney Cancer 8120613
2.2
3.3



Kidney Margin 8120614
7.3
14.9



Kidney Cancer 9010320
22.4
26.4



Kidney Margin 9010321
18.3
26.8



Normal Uterus
9.6
8.6



Uterine Cancer 064011
2.7
2.6



Normal Thyroid
5.1
5.3



Thyroid Cancer
39.2
44.1



Thyroid Cancer A302152
30.8
24.5



Thyroid Margin A302153
3.3
3.6



Normal Breast
5.0
4.5



Breast Cancer
0.8
1.7



Breast Cancer (OD04590-01)
9.7
7.6



Breast Cancer Mets (OD04590-03)
26.1
33.4



Breast Cancer Metastasis
3.4
4.1



Breast Cancer
3.3
4.4



Breast Cancer
7.2
8.3



Breast Cancer 9100266
3.1
3.7



Breast Margin 9100265
3.3
3.9



Breast Cancer A209073
8.2
8.9



Breast Margin A209073
16.2
11.6



Normal Liver
1.4
0.9



Liver Cancer
1.8
5.1



Liver Cancer 1025
1.3
2.0



Liver Cancer 1026
3.4
2.4



Liver Cancer 6004-T
1.6
0.4



Liver Tissue 6004-N
1.3
1.0



Liver Cancer 6005-T
0.0
6.5



Liver Tissue 6005-N
1.6
0.3



Normal Bladder
3.4
9.5



Bladder Cancer
10.7
6.5



Bladder Cancer
1.8
2.1



Bladder Cancer (OD04718-01)
1.7
5.2



Bladder Normal Adjacent (OD04718-03)
4.2
6.3



Normal Ovary
9.5
7.4



Ovarian Cancer
100.0
95.3



Ovarian Cancer (OD04768-07)
4.6
7.6



Ovary Margin (OD04768-08)
7.3
5.7



Normal Stomach
1.7
3.8



Gastric Cancer 9060358
2.1
0.5



Stomach Margin 9060359
1.6
3.1



Gastric Cancer 9060395
3.3
3.1



Stomach Margin 9060394
2.2
3.3



Gastric Cancer 9060397
11.0
14.5



Stomach Margin 9060396
2.8
4.3



Gastric Cancer 064005
2.2
6.5









Column A - Rel. Exp. (%) Ag2120, Run 149975659






Column B - Rel. Exp. (%) Ag2120, Run 150010138














TABLE BKD










Panel 4D










Tissue Name
A














Secondary Th1 act
0.4



Secondary Th2 act
0.8



Secondary Tr1 act
3.1



Secondary Th1 rest
3.4



Secondary Th2 rest
1.5



Secondary Tr1 rest
1.5



Primary Th1 act
0.3



Primary Th2 act
0.8



Primary Tr1 act
0.4



Primary Th1 rest
3.1



Primary Th2 rest
5.5



Primary Tr1 rest
0.6



CD45RA CD4 lymphocyte act
1.3



CD45RO CD4 lymphocyte act
2.4



CD8 lymphocyte act
2.2



Secondary CD8 lymphocyte rest
4.8



Secondary CD8 lymphocyte act
2.4



CD4 lymphocyte none
0.9



2ry Th1/Th2/Tr1 anti-CD95 CH11
0.7



LAK cells rest
7.2



LAK cells IL-2
0.4



LAK cells IL-2 + IL-12
2.8



LAK cells IL-2 + IFN gamma
7.7



LAK cells IL-2 + IL-18
4.1



LAK cells PMA/ionomycin
3.2



NK Cells IL-2 rest
2.3



Two Way MLR 3 day
2.7



Two Way MLR 5 day
4.0



Two Way MLR 7 day
5.2



PBMC rest
6.4



PBMC PWM
5.2



PBMC PHA-L
1.8



Ramos (B cell) none
3.9



Ramos (B cell) ionomycin
1.6



B lymphocytes PWM
0.0



B lymphocytes CD40L and IL-4
3.2



EOL-1 dbcAMP
67.4



EOL-1 dbcAMP PMA/ionomycin
56.6



Dendritic cells none
6.0



Dendritic cells LPS
15.5



Dendritic cells anti-CD40
13.5



Monocytes rest
46.0



Monocytes LPS
4.9



Macrophages rest
47.3



Macrophages LPS
24.8



HUVEC none
10.2



HUVEC starved
23.8



HUVEC IL-1beta
11.2



HUVEC IFN gamma
10.1



HUVEC TNF alpha + IFN gamma
4.1



HUVEC TNF alpha + IL4
13.2



HUVEC IL-11
8.1



Lung Microvascular EC none
3.2



Lung Microvascular EC TNFalpha + IL-1beta
2.4



Microvascular Dermal EC none
0.0



Microsvasular Dermal EC TNFalpha + IL-1beta
0.0



Bronchial epithelium TNFalpha + IL1beta
3.1



Small airway epithelium none
5.4



Small airway epithelium TNFalpha + IL-1beta
3.0



Coronery artery SMC rest
35.6



Coronery artery SMC TNFalpha + IL-1beta
39.0



Astrocytes rest
1.1



Astrocytes TNFalpha + IL-1beta
0.9



KU-812 (Basophil) rest
0.5



KU-812 (Basophil) PMA/ionomycin
2.0



CCD1106 (Keratinocytes) none
0.9



CCD1106 (Keratinocytes) TNFalpha + IL-1beta
2.6



Liver cirrhosis
5.5



Lupus kidney
9.3



NCI-H292 none
2.0



NCI-H292 IL-4
5.3



NCI-H292 IL-9
3.8



NCI-H292 IL-13
1.3



NCI-H292 IFN gamma
3.4



HPAEC none
9.0



HPAEC TNF alpha + IL-1 beta
15.4



Lung fibroblast none
1.7



Lung fibroblast TNF alpha + IL-1 beta
0.0



Lung fibroblast IL-4
2.8



Lung fibroblast IL-9
1.6



Lung fibroblast IL-13
1.0



Lung fibroblast IFN gamma
1.4



Dermal fibroblast CCD1070 rest
0.6



Dermal fibroblast CCD1070 TNF alpha
2.3



Dermal fibroblast CCD1070 IL-1 beta
5.4



Dermal fibroblast IFN gamma
0.5



Dermal fibroblast IL-4
3.0



IBD Colitis 2
5.8



IBD Crohn's
4.2



Colon
5.8



Lung
7.0



Thymus
100.0



Kidney
18.2









Column A - Rel. Exp. (%) Ag2120, Run 149975626














TABLE BKE










Panel CNS_1










Tissue Name
A














BA4 Control
46.3



BA4 Control2
42.9



BA4 Alzheimer's2
5.1



BA4 Parkinson's
38.4



BA4 Parkinson's2
100.0



BA4 Huntington's
53.6



BA4 Huntington's2
4.3



BA4 PSP
4.5



BA4 PSP2
14.1



BA4 Depression
11.8



BA4 Depression2
3.5



BA7 Control
45.4



BA7 Control2
37.9



BA7 Alzheimer's2
8.5



BA7 Parkinson's
11.7



BA7 Parkinson's2
54.3



BA7 Huntington's
41.2



BA7 Huntington's2
34.2



BA7 PSP
36.1



BA7 PSP2
21.2



BA7 Depression
4.5



BA9 Control
18.0



BA9 Control2
76.3



BA9 Alzheimer's
8.0



BA9 Alzheimer's2
4.6



BA9 Parkinson's
28.7



BA9 Parkinson's2
55.5



BA9 Huntington's
34.2



BA9 Huntington's2
4.7



BA9 PSP
11.6



BA9 PSP2
3.8



BA9 Depression
5.0



BA9 Depression2
3.2



BA17 Control
36.9



BA17 Control2
62.0



BA17 Alzheimer's2
2.9



BA17 Parkinson's
13.4



BA17 Parkinson's2
61.1



BA17 Huntington's
27.9



BA17 Huntington's2
8.4



BA17 Depression
2.3



BA17 Depression2
10.1



BA17 PSP
20.0



BA17 PSP2
10.5



Sub Nigra Control
10.6



Sub Nigra Control2
29.1



Sub Nigra Alzheimer's2
5.8



Sub Nigra Parkinson's2
20.6



Sub Nigra Huntington's
21.5



Sub Nigra Huntington's2
14.7



Sub Nigra PSP2
3.6



Sub Nigra Depression
3.2



Sub Nigra Depression2
2.4



Glob Palladus Control
1.8



Glob Palladus Control2
7.8



Glob Palladus Alzheimer's
6.4



Glob Palladus Alzheimer's2
2.5



Glob Palladus Parkinson's
34.4



Glob Palladus Parkinson's2
3.1



Glob Palladus PSP
5.2



Glob Palladus PSP2
0.0



Glob Palladus Depression
3.7



Temp Pole Control
11.0



Temp Pole Control2
40.1



Temp Pole Alzheimer's
2.7



Temp Pole Alzheimer's2
3.4



Temp Pole Parkinson's
13.9



Temp Pole Parkinson's2
20.7



Temp Pole Huntington's
27.5



Temp Pole PSP
2.5



Temp Pole PSP2
3.7



Temp Pole Depression2
1.8



Cing Gyr Control
70.7



Cing Gyr Control2
23.8



Cing Gyr Alzheimer's
13.8



Cing Gyr Alzheimer's2
7.7



Cing Gyr Parkinson's
13.2



Cing Gyr Parkinson's2
23.0



Cing Gyr Huntington's
40.3



Cing Gyr Huntington's2
12.9



Cing Gyr PSP
7.6



Cing Gyr PSP2
5.6



Cing Gyr Depression
4.1



Cing Gyr Depression2
4.6









Column A - Rel. Exp. (%) Ag2120, Run 171649389







Panel 1.3D Summary: Ag2120 Expression of this gene was highest in the cerebral cortex (CT value=29). Moderate expression was detected in all other regions of the brain except thalamus and substantia nigra, demonstrating that this gene is associated with normal brain homeostasis. This protein showed a brain-preferential expression, see write-up on Panel CNS.01 for discussion of utility. In addition, expression of this gene was down-regulated in CNS cancer cell lines. Over-expression of this gene was also detected in several lung cancer cell lines relative to normal control. Therefore, this gene is useful for the detection or treatment of CNS and lung cancers.


Panel 2D Summary: Ag2120 Expression of this gene in panel 2D revealed an association of expression in thyroid, breast and kidney cancers when compared to their respective normal adjacent tissues. Thus, therapeutic modulation of this gene with inhibitory monoclonal antibodies and/or small molecule therapeutics is useful in treatment of these diseases. In addition, the SC98428706_EXT gene might be useful as a marker for thyroid, breast and kidney cancers.


Panel 4D Summary: Ag 2120 This gene showed highest expression levels in the thymus. (CT value=31). The transcript was also expressed in eosinophils, monocytes, macrophages and coronary artery. It was down regulated in LPS-treated monocytes and to a lesser degree in LPS treated macrophages. Therefore, protein therapeutics (agonists or antagonists) designed against the protein encoded for by this transcript are useful for reducing the inflammatory process observed in asthma, emphysema, osteoarthritis and sepsis.


Panel CNS—1 Summary: Ag2120 Insulin and insulin-like growth factors belong to a family of polypeptides essential for proper regulation of physiologic processes such as energy metabolism, cell proliferation, development, and differentiation. The insulin-like growth factors bind to IGF with high affinity and compete with the IGF receptor for IGF binding. Transgenic mice over expressing insulin-like growth factor binding proteins (IGFBPs) tend to show brain developmental abnormalities, suggesting a role for these proteins in neurodevelopment. Furthermore, treatment with glycosaminoglycans (which increases muscle re-innervation after motor neuron death) upregulates serum levels of both IGF and IGFBP. Thus, the novel IGFBP encoded by this gene is useful in the treatment of diseases such as ALS, multiple sclerosis, and peripheral nerve injury on the basis of its homology to other established IGFBPS. The expression profile of this gene shows that it is expressed preferentially in the brain, with highest levels in the cerebral cortex and hippocampus, two regions that are known to degenerate in Alzheimer's disease. Examination of the expression profile on Panel CNS.01 showed that most regions of both control and diseased brains express this protein; however the levels were decreased in the motor cortex in progressive supranuclear palsy and depression. Thus, this protein is of use in the treatment of Alzheimer's disease, progressive supranuclear palsy, and depression.


Example D
CG56008, NOV53
DETAILED DESCRIPTION

The present invention is based in part on the discovery that reducing the amount of LIV-1 mRNA present in tumor derived cells results in strongly reduced cell growth. The expression of this gene in tumor-derived cells was first defined using RTQPCR analysis.


The RTQPCR analysis of panel 1.3d reveals that this gene has a restricted high expression in 5 breast cancer cell lines, with the highest expression in MCF-7 breast cancer cells. This gene is present in normal tissues at low levels (FIG. 1). The specific high expression in breast cancer cell lines indicates a role in breast cancer progression. This is further supported by the analysis of panel 2d that reveals how LIV-1 is upregulated specifically in breast cancers, comparing to the normal adjacent tissues (FIG. 2). Based upon its profile, the expression of this gene could be of use as a marker for the presence of breast cancer in a subject, specifically in a subject blood. In addition, down-regulation of the activity of this gene, through the use of antibodies or antisense oligonucleotide, might be of use in the treatment of breast cancer.


The expression profiles of this gene on our RTQPCR panels indicate that this gene may be involved in regulating cancer cell proliferation and oncogenesis. To confirm whether LIV-1 expression is required for cell growth, we used antisense technology to examine the effect of decreasing LIV-1 gene expression on breast cancer cell proliferation. This technology has been used to asses the biological/therapeutic effect of reducing (knockdown) the expression of a gene (see Tamm et al., Lancet 2001 August 11;358(9280):489-97 for a review of the current use of this technology in the biomedical field)


As shown in FIG. 4, transfection of LIV-1 antisense oligonucleotide AS4 at 400 nM concentration inhibited T47D breast carcinoma cell growth more than 50% (P=0.000189), whereas transfection of scramble antisense control had little effect on cell growth. We observed less inhibitory effect with LIV-1 antisense oligo AS4 transfection in Ovcar4 cells in which the expression of LIV-1 is much lower (P=0.138366) (FIG. 5). Knockdown of LIV-1 transcripts by antisense oligonucleotides was validated by RTQPCR. As shown in FIG. 6, transfection of antisense oligo AS4 resulted in 50% knockdown of LWV-1 expression at the mRNA level.


Therefore, our results suggested that LIV-1 expression is involved in cell proliferation. The inhibition of the activity of this gene through the use of antibodies or antisense oligonucleotides, mnight be of use in the treatment of breast cancer.


Example D1
Expression Analysis of LIV-1 in Various Tissues

RTOPCR Probe/Primer Information for LIV-1


RTQPCR Probe/Primers Set Ag2169

(SEQ ID NO: 1603)Ag 2169 (F):5′-CCCGAAAAGGCTTTATGTATTC-3′(SEQ ID NO: 1604)Ag 2169 (R):5′-TGTCAGTAGCTTTGATGCATTG-3′(SEQ ID NO: 1605)Ag 2169 (P):FAM-5′-CAGAAACACAAATGAAAATCCTCAGGA-3′-TAMRA









TABLE D1










RTQPCR results for Panel 1.3d.










Rel. Expr., %
Rel. Expr., %



1.3dtm3016f—
1.3Dtm3183f—


Tissue Name
ag2169
ag2169












Liver adenocarcinoma
1.8
2


Pancreas
1
0.4


Pancreatic ca. CAPAN 2
1
1


Adrenal gland
0.8
0.6


Thyroid
2
0.9


Salivary gland
1.2
0.8


Pituitary gland
3.1
2.2


Brain (fetal)
2.2
1.7


Brain (whole)
2.6
2.1


Brain (amygdala)
2
1.1


Brain (cerebellum)
1.4
0.9


Brain (hippocampus)
6.1
4.4


Brain (substantia nigra)
0.5
0.8


Brain (thalamus)
2.5
2


Cerebral Cortex
2.8
3.1


Spinal cord
1.6
1.4


glio/astro U87-MG
1.2
0.8


glio/astro U-118-MG
12
9.3


astro SW1783
2.8
3


neuro; met SK-N-AS
10.7
6.7


astro SF-539
1.7
1.5


astro SNB-75
2.8
3.8


glio SNB-19
1
0.9


glio U251
0.8
0.8


glio SF-295
3.4
3


Heart (fetal)
0.4
0.5


Heart
0.2
0.1


Fetal Skeletal
1.2
1.4


Skeletal muscle
0.2
0.2


Bone marrow
0.4
0.2


Thymus
0.3
0.3


Spleen
1.1
0.8


Lymph node
0.8
0.5


Colorectal
0.3
0.2


Stomach
1.5
0.8


Small intestine
0.9
0.5


Colon SW480
1.6
1.2


Colon SW620(SW480 met)
0.7
0.5


Colon HT29
0.8
0.6


Colon HCT-116
4.2
3.1


Colon CaCo-2
0.9
1.1


Colon Ca tissue(ODO3866)
1.3
1.2


Colon HCC-2998
2.1
1.6


Gastric(liver met) NCI-N87
2
1.6


Bladder
1
0.6


Trachea
1.6
1.6


Kidney
0.5
0.5


Kidney (fetal)
1.1
0.8


Renal 786-0
2.6
1.7


Renal A498
4.2
3.2


Renal RXF 393
1.2
0.8


Renal ACHN
2.6
2.7


Renal UO-31
3.3
2.4


Renal TK-10
2
1.5


Liver
0.1
0


Liver (fetal)
0.5
0.3


Liver (hepatoblast) HepG2
1.5
1.3


Lung
0.8
0.6


Lung (fetal)
1.5
1.5


Lung (small cell) LX-1
1
0.7


Lung (small cell) NCI-H69
10
6.3


Lung (s. cell var.) SHP-77
3.9
4.9


Lung (large cell)NCI-H460
1.3
1.2


Lung (non-sm. cell) A549
0.9
0.6


Lung (non-s. cell) NCI-H23
5.4
0


Lung (non-s. cell) HOP-62
1.8
2


Lung (non-s. cl) NCI-H522
1.8
1.2


Lung (squam.) SW 900
1.2
0.8


Lung (squam.) NCI-H596
3.1
3


Mammary gland
11.7
10.4


Breast (pl. ef) MCF-7
100
100


Breast (pl.ef) MDA-MB-231
2.5
2.1


Breast (pl.ef) T47D
5.7
3.3


Breast BT-549
4.5
3.6


Breast MDA-N
2.6
2.8


Ovary
2
1.3


Ovarian OVCAR-3
2.2
2


Ovarian OVCAR-4
0.3
0.2


Ovarian OVCAR-5
0.6
0.5


Ovarian OVCAR-8
1.6
0.9


Ovarian IGROV-1
0.8
0.5


Ovarian (ascites) SK-OV-3
4
3.2


Uterus
1.1
0.8


Plancenta
3.4
2.1


Prostate
5.5
4.6


Prostate (bone met)PC-3
2
1.3


Testis
1.9
1.6


Melanoma Hs688(A).T
4.8
4.8


Melanoma (met) Hs688(B).T
6.2
5.2


Melanoma UACC-62
0.3
0.3


Melanoma M14
2.8
2.6


Melanoma LOX IMVI
0.6
0.4


Melanoma (met) SK-MEL-5
7.1
5.1


Adipose
1.2
0.8
















TABLE D2










RTQPCR results for Panel 2d.










Rel. Expr., %
Rel. Expr., %


Tissue Name
2Dtm2969f_ag2169
2dtm3017f_ag2169












Normal Colon
3.2
3.1


CCa 1
0.6
0.5


CCa 1 Margin
0.2
0.4


CCa 2
0.1
0.2


CCa 2 Margin
0.2
0.1


CCa 3
0.2
0.2


CCa 3 Margin
0.3
0.2


CCa 4
1
1.1


CCa 4 Margin
0.3
0.4


CCa 5 Metastasis
1.6
1.8


CCa 5 Margin (Liver)
0.5
0.4


CCa 6 Metastatsis
0.2
0.2


CCa 6 Margin (Lung)
0.4
0.3


Normal Prostate
7.7
7.9


PCa 1
15.1
18.7


PCa 1 Margin
7.4
7.9


PCa 2
3.4
4


PCa 2 Margin
6.7
7.3


Normal Lung
1.4
1.6


LCa 1 Metastasis
1.4
1.5


LCa 1 Margin (muscle)
0.7
0.7


LCa 2
1.7
2


LCa 2 Margin
1.1
1.3


LCa 3
2
2.6


LCa 3 Margin
1
1.2


LCa 4
1
1.1


LCa 4 Margin
0.5
0.5


LCa 5
3.1
3.4


LCa 5 Margin
0.9
1


Ocular Melanoma Metastasis
3.7
4.1


Liver Margin
0.2
0.4


Melanoma Metastasis
3.5
4


Lung Margin
0.9
1.4


Normal Kidney
2.5
3.1


RCC 1
2.8
2.8


RCC 1 Margin
1.8
1.9


RCC 2
0.7
0.6


RCC 2 Margin
1.4
1.4


RCC 3
2.5
3.1


RCC 3 Margin
1.8
1.9


RCC 4
1
0.9


RCC 4 Margin
1.5
1.7


RCC 5
0.9
0.9


RCC 5 Margin
0.2
0.1


RCC 6
1.1
1


RCC 6 Margin
1.4
1.5


RCC 7
0.5
0.4


RCC 7 Margin
0.3
0.2


RCC 8
0.4
0.4


RCC 8 Margin
0.2
0.2


RCC 9
0.8
0.9


RCC 9 Margin
0.5
0.6


Normal Uterus
0
0.4


UtCa
1.8
1.9


Normal Thyroid
1.4
1.8


ThyCa 1
1.7
1.9


ThyCa 2
0.9
0.9


ThyCa 2 Margin
1.5
1.5


Normal Breast
3.9
4.9


BCa 1
19.8
26.2


BCa 2
46.7
45.7


BCa 3 Metastasis
43.2
57.8


BCa 4 Metastasis
100
100


BCa 5
2.4
2.7


BCa 6
2.5
2.3


BCa 7
41.2
45.7


BCa 7 Margin
5
5.7


BCa 8
4
5.4


BCa 8 Margin
4.1
6.8


Normal Liver
0.2
0.3


HCC 1
0.2
0.2


HCC 2
0.2
0.1


HCC 3
0.3
0.2


HCC 4
0.2
0.2


HCC 4 Margin
0.5
0.5


HCC 5
0.2
0.2


HCC 5 Margin
0
0


Normal Bladder
1.5
1.4


TCC 1
0.3
0.2


TCC 2
1.7
1.8


TCC 3
3
3.3


TCC 3 Margin
2.9
1.4


Normal Ovary
0.3
0.3


OVCa 1
3.3
3


OVCa 2
3.1
3.1


OVCa 2 Margin
0.4
0.5


Normal Stomach
0.5
0.5


GaCa 1
0.2
0.2


GaCa 1 Margin
0.4
0.4


GaCa 2
0.8
0.7


GaCa 2 Margin
0.5
0.5


GaCa 3
1
0.9


GaCa 3 Margin
0.1
0.1


GaCa 4
1
0.8









Example D2
Antisense knockdown of LIV-1 mRNA in High-expressing and Low-expressing Tumor-derived Cell Lines

Five oligonucleotides were designed and synthesized as mixed-backbone oligonucleotides containing modified phosphorothioate segments at 5′ and 3′ ends and 2′-O-methyl RNA oligoribonucleotide segments located in the middle. The purity of the oligonucleotides was confirmed by Masspectrophometry. The oligonucleotide sequences for LIV-1 are: ASI: 5′ GATCCAGGCTCTGTCACTCAC 3′ (SEQ ID NO: 1606) (complementary to the sequence located in 5′ UTR of LIV-1). AS2: 5′ ATTATGGGATCAGCGTTC 3′ (SEQ ID NO: 1607) (complementary to the sequence surrounding ATG start codon). AS3: 5′ AGGCTTAAGATGACCACTRG 3′ (complementary to the sequence 3′ next to AS2). AS4: 5′ GTGGTGCTCGTIFCGAACAGT 3′ (SEQ ID NO: 1608) (complementary to the sequence in the middle of SLPI ORF). AS5: 5° CTGGGTCTTAGTCCGTGGCA 3′ (SEQ ID NO: 1609) (complementary to the sequence flanking the 3′ stop codon). 10,000 cells were seeded in each well of 96 well plate in compelete medium 24 hr before transfection to reach 50% confluency on the day of transfection. Oligonucleotides were diluted with Optimen to 100 and 400 nM, and mixed with Oligofectamine (Invitrogen) according to manufecture's instructions. Cells were washed with serum-free medium. The oligo and lipisome mixture were then added to cells. After 4 hr incubation period, serum were added back to cells. Readout assays were performed 24 and 48 hr after transfection. CellTiter 96Aqueous Non-Radioactive Cell Porliferation Assay Kit from Promega was used to determine the number of viable cells in proliferation assay. Briefly, 20 μl of combined MTS/PMS solution were diluted with 100 μl complete medium, and added to each well of the 96 well plate. After 1 hr incubation at 37° C., the absorbance at 490 nm was recorded using an ELISA plate reader.



FIG. 2. Antisense Knockdown of LIV-1 Inhibits T47D Cell Growth.


Breast cancer cell line T47D were transfected with 5 LIV-1 antisense oligonucleotides either individually or in the combinations as indicated. 72 hr after transfection, MTS assay was performed. The experiment was repeated twice with similar results. The representative data is shown. Asterisk indicates statistically significant difference on Student's 2-samples t test assuming unequal variances. P value for knockdown with AS4 comparing to scramble control at 400 nM is 0.000189.



FIG. 3. Antisense Knockdown of LIV-1 Onhibits Ovcar-4 Cell Growth.


Ovarian cancer cell line Ovcar4 were transfected with LWV-1 antisense oligonucleotides either individually or in the combinations as indicated. 72 hr after transfection, MTS assay was performed. Asterisk indicates statistically significant difference on Student's 2-samples t test assuming unequal variances. P value for knockdown with AS4 comparing to scramble control at 400 nM is 0.138366.



FIG. 4. RTOPCR Validation of Knockdown of LIV-1 Expression at mRNA Level in T47D Breast Cancer Cell Line.


T47D cells were transfected with LIV-1 antisense oligonucleotides in the combinations as indicated. 24 hr after transfection, cells were lysed in cell lysis buffer, and total RNA was isolated using RNAeasy kit from Qiagen. After DNAse digestion, cDNA was synthesized using Superscript II reverse transcriptase (BRL). RTQ-PCR analysis was performed according to the standard RTQPCR operation procedures.


Other Embodiments

Although particular embodiments are disclosed herein in detail, this is done by way of example for purposes of illustration only, and is not intended to be limiting with respect to the scope of the appended claims, which follow. In particular, it is contemplated by the inventors that various substitutions, alterations, and modifications will be made to the invention without departing from the spirit and scope of the invention as defined by the claims. The choice of nucleic acid starting material, clone of interest, or library type is believed to be a matter of routine for a person of ordinary skill in the art with knowledge of the embodiments described herein. Other aspects, advantages, and modifications considered to be within the scope of the following claims. The claims presented are representative of the inventions disclosed herein. Other, unclaimed inventions are also contemplated. Applicants reserve the right to pursue such inventions in later claims.

Claims
  • 1. An isolated polypeptide comprising the mature form of an amino acid sequenced selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606.
  • 2. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606.
  • 3. An isolated polypeptide comprising an amino acid sequence which is at least 95% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606.
  • 4. An isolated polypeptide, wherein the polypeptide comprises an amino acid sequence comprising one or more conservative substitutions in the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606.
  • 5. The polypeptide of claim 1 wherein said polypeptide is naturally occurring.
  • 6. A composition comprising the polypeptide of claim 1 and a carrier.
  • 7. A kit comprising, in one or more containers, the composition of claim 6.
  • 8. The use of a therapeutic in the manufacture of a medicament for treating a syndrome associated with a human disease, the disease selected from a pathology associated with the polypeptide of claim 1, wherein the therapeutic comprises the polypeptide of claim 1.
  • 9. A method for determining the presence or amount of the polypeptide of claim 1 in a sample, the method comprising: (a) providing said sample; (b) introducing said sample to an antibody that binds immunospecifically to the polypeptide; and (c) determining the presence or amount of antibody bound to said polypeptide, thereby determining the presence or amount of polypeptide in said sample.
  • 10. A method for determining the presence of or predisposition to a disease associated with altered levels of expression of the polypeptide of claim 1 in a first mammalian subject, the method comprising: a) measuring the level of expression of the polypeptide in a sample from the first mammalian subject; and b) comparing the expression of said polypeptide in the sample of step (a) to the expression of the polypeptide present in a control sample from a second mammalian subject known not to have, or not to be predisposed to, said disease, wherein an alteration in the level of expression of the polypeptide in the first subject as compared to the control sample indicates the presence of or predisposition to said disease.
  • 11. A method of identifying an agent that binds to the polypeptide of claim 1, the method comprising: (a) introducing said polypeptide to said agent; and (b) determining whether said agent binds to said polypeptide.
  • 12. The method of claim 11 wherein the agent is a cellular receptor or a downstream effector.
  • 13. A method for identifying a potential therapeutic agent for use in treatment of a pathology, wherein the pathology is related to aberrant expression or aberrant physiological interactions of the polypeptide of claim 1, the method comprising: (a) providing a cell expressing the polypeptide of claim 1 and having a property or function ascribable to the polypeptide; (b) contacting the cell with a composition comprising a candidate substance; and (c) determining whether the substance alters the property or function ascribable to the polypeptide; whereby, if an alteration observed in the presence of the substance is not observed when the cell is contacted with a composition in the absence of the substance, the substance is identified as a potential therapeutic agent.
  • 14. A method for screening for a modulator of activity of or of latency or predisposition to a pathology associated with the polypeptide of claim 1, said method comprising: (a) administering a test compound to a test animal at increased risk for a pathology associated with the polypeptide of claim 1, wherein said test animal recombinantly expresses the polypeptide of claim 1;(b) measuring the activity of said polypeptide in said test animal after administering the compound of step (a); and (c) comparing the activity of said polypeptide in said test animal with the activity of said polypeptide. in a control animal not administered said polypeptide, wherein a change in the activity of said polypeptide in said test animal relative to said control animal indicates the test compound is a modulator activity of or latency or predisposition to, a pathology associated with the polypeptide of claim 1.
  • 15. The method of claim 14, wherein said test animal is a recombinant test animal that expresses a test protein transgene or expresses said transgene under the control of a promoter at an increased level relative to a wild-type test animal, and wherein said promoter is not the native gene promoter of said transgene.
  • 16. A method for modulating the activity of the polypeptide of claim 1, the method comprising contacting a cell sample expressing the polypeptide of claim 1 with a compound that binds to said polypeptide in an amount sufficient to modulate the activity of the polypeptide.
  • 17. A method of treating or preventing a pathology associated with the polypeptide of claim 1, the method comprising administering the polypeptide of claim 1 to a subject in which such treatment or prevention is desired in an amount sufficient to treat or prevent the pathology in the subject.
  • 18. The method of claim 17, wherein the subject is a human.
  • 19. A method of treating a pathological state in a mammal, the method comprising administering to the mammal a polypeptide in an amount that is sufficient to alleviate the pathological state, wherein the polypeptide is a polypeptide having an amino acid sequence at least 95% identical to a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606 or a biologically active fragment thereof.
  • 20. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606.
  • 21. The nucleic acid molecule of claim 20, wherein the nucleic acid molecule is naturally occurring.
  • 22. A nucleic acid molecule, wherein the nucleic acid molecule differs by a single nucleotide from a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2n−1, wherein n is an integer between 1 and 606.
  • 23. An isolated nucleic acid molecule encoding the mature form of a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 606.
  • 24. An isolated nucleic acid molecule comprising a nucleic acid selected from the group consisting of 2n−1, wherein n is an integer between 1 and 606.
  • 25. The nucleic acid molecule of claim 20, wherein said nucleic acid molecule hybridizes under stringent conditions to the nucleofide sequence selected from the group consisting of SEQ ID NO: 2n−1, wherein n is an integer between 1 and 606, or a complement of said nucleotide sequence.
  • 26. A vector comprising the nucleic acid molecule of claim 20.
  • 27. The vector of claim 26, further comprising a promoter operably linked to said nucleic acid molecule.
  • 28. A cell comprising the vector of claim 26.
  • 29. An antibody that immunospecifically binds to the polypeptide of claim 1.
  • 30. The antibody of claim 29, wherein the antibody is a monoclonal antibody.
  • 31. The antibody of claim 29, wherein the antibody is a humanized antibody.
  • 32. A method for determining the presence or amount of the nucleic acid molecule of claim 20 in a sample, the method comprising: (a) providing said sample; (b) introducing said sample to a probe that binds to said nucleic acid molecule; and (c) determining the presence or amount of said probe bound to said nucleic acid molecule, thereby determining the presence or amount of the nucleic acid molecule in said sample.
  • 33. The method of claim 32 wherein presence or amount of the nucleic acid molecule is used as a marker for cell or tissue type.
  • 34. The method of claim 33 wherein the cell or tissue type is cancerous.
  • 35. A method for determining the presence of or predisposition to a disease associated with altered levels of expression of the nucleic acid molecule of claim 20 in a first mammalian subject, the method comprising: a) measuring the level of expression of the nucleic acid in a sample from the first mammalian subject; and b) comparing the level of expression of said nucleic acid in the sample of step (a) to the level of expression of the nucleic acid present in a control sample from a second mammalian subject known not to have or not be predisposed to, the disease; wherein an alteration in the level of expression of the nucleic acid in the first subject as compared to the control sample indicates the presence of or predisposition to the disease.
  • 36. A method of producing the polypeptide of claim 1, the method comprising culturing a cell under conditions that lead to expression of the polypeptide, wherein said cell comprises a vector comprising an isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606.
  • 37. The method of claim 36 wherein the cell is a bacterial cell.
  • 38. The method of claim 36 wherein the cell is an insect cell.
  • 39. The method of claim 36 wherein the cell is a yeast cell.
  • 40. The method of claim 36 wherein the cell is a mammalian cell.
  • 41. A method of producing the polypeptide of claim 2, the method comprising culturing a cell under conditions that lead to expression of the polypeptide, wherein said cell comprises a vector comprising an isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:2n−1, wherein n is an integer between 1 and 606.
  • 42. The method of claim 41 wherein the cell is a bacterial cell.
  • 43. The method of claim 41 wherein the cell is an insect cell.
  • 44. The method of claim 41 wherein the cell is a yeast cell.
  • 45. The method of claim 41 wherein the cell is a mammalian cell.
RELATED APPLICATIONS

This application is a continuation-in-part of U.S. Ser. No. 09/789,390, filed Feb. 23, 2001, which claims priority to U.S. Ser. No. 60/185,967, filed Mar. 1, 2000; U.S. Ser. No. 09/823,187, filed Mar. 29, 2001, which claims priority to U.S. Ser. No. 60/195,792, filed Mar. 10, 2000; U.S. Ser. No. 09/839,446, filed Mar. 19, 2001, which claims priority to U.S. Ser. No. 60/199,476, filed Mar. 25, 2000; and U.S. Ser. No. 60/199,476, filed Apr. 25, 20001; U.S. Ser. No. 09/863,776, filed May 23, 2001, which claims priority to U.S. Ser. No. 60/208,263, filed May 31, 2000; U.S. Ser. No. 09/939,398, filed Aug. 24, 2001, which claims priority to U.S. Ser. No. 60/227,800, filed Aug. 25, 2000; U.S. Ser. No. 09/964,956, filed Sep. 26, 2001, which claims priority to U.S. Ser. No. 60/236,135, filed Sep. 28, 2000; U.S. Ser. No. 10/015,115, filed Nov. 13, 2001, which claims priority to U.S. Ser. No. 60/248,153, filed Nov. 13, 2000; U.S. Ser. No. 10/028,248, filed Dec. 19, 2001, which claims priority to U.S. Ser. No. 60/285,189, filed Apr. 20, 2001; U.S. Ser. No. 10/032,189, filed Dec. 21, 2001, which claims priority to U.S. Ser. No. 60/258,171, filed Dec. 22, 2000; and U.S. Ser. No. 60/257,495, filed Dec. 21, 2000; U.S. Ser. No. 10/055,877, filed Jan. 22, 2002, which claims priority to U.S. Ser. No. 60/296,960, filed Jun. 8, 2001 and U.S. Ser. No. 60/335,669, filed Oct. 31, 2001; U.S. Ser. No. 10/072,012, filed Jan. 31, 2002, which claims priority to U.S. Ser. No. 60/265,412, filed Jan. 31, 2001; U.S. Ser. No. 10/080,334, filed Feb. 21, 2002, which claims priority to U.S. Ser. No. 60/270,220, filed Feb. 21, 2001; U.S. Ser. No. 10/093,463, filed Mar. 8, 2002, which claims priority to U.S. Ser. No. 60/277,311, filed Mar. 20, 2001; U.S. Ser. No. 10/114,153, filed Apr. 2, 2002, which claims priority to U.S. Ser. No. 60/283,678, filed Apr. 13, 2001; and U.S. Ser. No. 60/286,292, filed Apr. 25, 2001; U.S. Ser. No. 10/114,270, filed Apr. 2, 2002, which claims priority to U.S. Ser. No. 60/281,136, filed Apr. 3, 2001; U.S. Ser. No. 10/138,588, filed Apr. 1, 2002 which claims priority to U.S. Ser. No. 60/288,900, filed May 4, 2001; U.S. Ser. No. 09/783,429, filed Feb. 14, 2001; U.S. Ser. No. 09/800,198, filed Mar. 5, 2001; U.S. Ser. No. 10/023,634, filed Dec. 17, 2001; U.S. Ser. No. 10/038,854, filed Dec. 31, 2001; U.S. Ser. No. 10/051,874, filed Jan. 16, 2002; and U.S. Ser. No. 10/072,013, filed Feb. 8, 2002; and claims the benefit of U.S. Ser. No. 60/386,376, filed Jun. 6, 2002; U.S. Ser. No. 60/385,784, filed Jun. 4, 2002;U.S. Ser. No. 60/386,453, filed Jun. 6, 2002; U.S. Ser. No. 60/402,389, filed Aug. 9, 2002; U.S. Ser. No. 60/412,528, filed Sep. 20, 2002; U.S. Ser. No. 60/387,016, filed Jun. 6, 2002; U.S. Ser. No. 60/386,796, filed Jun. 7, 2002; U.S. Ser. No. 60/386,971, filed Jun. 7, 2002; U.S. Ser. No. 60/386,041, filed Jun. 5, 2002; U.S. Ser. No. 60/387,610, filed Jun. 11, 2002; U.S. Ser. No. 60/386,047, filed Jun. 5, 2002; U.S. Ser. No. 60/387,400, filed Jun. 10, 2002; U.S. Ser. No. 60/402,156, filed Aug. 9, 2002; U.S. Ser. No. 60/386,816, filed Jun. 7, 2002; U.S. Ser. No. 60/402,256, filed Aug. 9, 2002; U.S. Ser. No. 60/389,144, filed Jun. 14, 2002; U.S. Ser. No. 60/386,942, filed Jun. 7, 2002; U.S. Ser. No. 60/387,262, filed Jun. 7, 2002; U.S. Ser. No. 60/389,120, filed Jun. 14, 2002; U.S. Ser. No. 60/421,156, filed Oct. 28, 2002; U.S. Ser. No. 60/423,130, filed Nov. 1, 2002; U.S. Ser. No. 60/425,453, filed Nov. 12, 2002; U.S. Ser. No. 60/386,931, filed Jun. 7, 2002; U.S. Ser. No. 60/403,459, filed Aug. 13, 2002; U.S. Ser. No. 60/387,535, filed Jun. 10, 2002; U.S. Ser. No. 60/403,448, filed Aug. 13, 2002; U.S. Ser. No. 60/423,798, filed Nov. 5, 2002; U.S. Ser. No. 60/387,859, filed Jun. 11, 2002; U.S. Ser. No. 60/402,821, filed Aug. 12, 2002; U.S. Ser. No. 60/417,186, filed Oct. 9, 2002; U.S. Ser. No. 10/015,115, filed Nov. 13, 2001; U.S. Ser. No. 60/387,702, filed Jun. 11, 2002; U.S. Ser. No. 60/387,634, filed Jun. 11, 2002; U. S.S. N. 60/387,934, filed Jun. 12,2002; U.S. Ser. No. 60/387,696, filed Jun. 11, 2002; U.S. Ser. No. 60/390,006, filed Jun. 19, 2002; U.S. Ser. No. 60/387,668, filed Jun. 11, 2002; U.S. Ser. No. 60/402,786, filed Aug. 12, 2002; U.S. Ser. No. 60/410,084, filed Sep. 1 2, 2002 ; U.S. Ser. No. 60/420,639, filed Oct. 23, 2002; U.S. Ser. No. 60/386,864, filed Jun. 6, 2002; U.S. Ser. No. 60/401,628, filed Aug. 6, 2002; U.S. Ser. No. 60/406,182, filed Aug. 26, 2002; U.S. Ser. No. 60/387,836, filed Jun. 11, 2002; U.S. Ser. No. 60/389,729, filed Jun. 17, 2002; U.S. Ser. No. 60/388,022, filed Jun. 12, 2002; U.S. Ser. No. 60/402,816, filed Aug. 12, 2002; U.S. Ser. No. 60/412,731, filed Sep. 23, 2002; U.S. Ser. No. 60/414,954, filed Sep. 30, 2002; U.S. Ser. No. 60 /388,096, filed Jun. 12, 2002; U.S. Ser. No. 60/414,840, filed Sep. 30, 2002; U.S. Ser. No. 60/417,406, filed Oct. 9, 2002; U.S. Ser. No. 60/389,123, filed Jun. 13, 2002; U.S. Ser. No. 60/402,832, filed Aug. 12, 2002; U.S. Ser. No. 60/403,532, filed Aug. 13, 2002; U.S. Ser. No. 60/414,839, filed Sep. 30, 2002; U.S. Ser. No. 60/387,625, filed Jun. 11, 2002; U.S. Ser. No. 60/390,209, filed Jun. 19, 2002; U.S. Ser. No. 60/403,531, filed Aug. 13, 2002; U.S. Ser. No. 60/387,933, filed Jun. 12, 2002; U.S. Ser. No. 60/406,355, filed Aug. 26, 2002; U.S. Ser. No. 10/094,886, filed Mar. 7, 2002; U.S. Ser. No. 60/389,118, filed Jun. 14, 2002; U.S. Ser. No. 60/406,317, filed Aug. 13, 2002; U.S. Ser. No. 60/403,563, filed Aug. 13, 2002; U.S. Ser. No. 60/389,884, filed Jun. 18, 2002; U.S. Ser. No. 60/406,240, filed Aug. 27, 2002; U.S. Ser. No. 60/389,146, filed Jun. 14, 2002; U.S. Ser. No. 60/387,960, filed Jun. 12, 2002; U.S. Ser. No. 60/403,617, filed Aug. 15, 2002; U.S. Ser. No. 60/348,693, filed Jan. 14, 2002; U.S. Ser. No. 60/390,763, filed Jun. 21, 2002; U.S. Ser. No. 60/385,120, filed Jun. 3, 2002; U.S. Ser. No. 60/414,801, filed Sep. 30, 2002; U.S. Ser. No. 60/422,690, filed Oct. 31, 2002; U.S. Ser. No. 60/389,742, filed Jun. 17, 2002; U.S. Ser. No. 60/396,706, filed Jul. 17, 2002.

Provisional Applications (1)
Number Date Country
60185967 Mar 2000 US
Continuation in Parts (1)
Number Date Country
Parent 09789390 Feb 2001 US
Child 10453372 Jun 2003 US