Claims
- 1. A method for reducing the thermal stability of Bacillus serine protease by acylation, comprising acylating said protease with an acyl radical of a monocarboxylic or dicarboxylic acid of about 1 to 6 carbon atoms to a reduced thermal stability of at least about 3.degree. C. and having at least about 50% of the proteolytic activity before acylating.
- 2. The method of claim 1 wherein said reduced thermal stability is from about 3.degree. C. to about 15.degree. C.
- 3. The method of claim 1 wherein said reduced thermal stability is from about 5.degree. C. to about 15.degree. C.
- 4. The method of claim 1 wherein said activity is at least about 70% of the proteolytic before acylating.
- 5. The method of claim 1 wherein said activity is at least about 90% of the proteolytic activity before acylating.
- 6. The method of claim 1 wherein said acid is a monocarboxylic acid selected from the group consisting of acetic, propionic, butyric.
- 7. The method of claim 1 wherein said acid is a dicarboxylic acid selected from the group consisting of maleic and succinic acids and mixtures thereof.
- 8. The method claim 1 wherein said protease is subtilisin Carlsberg.
- 9. A thermally destabilized Bacillus serine protease acylated with an acylating derivative of a monocarboxylic acid containing about 1 to 6 carbon atoms to a reduced thermal stability of at least about 3.degree. C. at pH 7.5 and having at least about 50% of the proteolytic activity before acylating.
- 10. The protease of claim 9 wherein said protease is subtilisin Carlsberg.
Priority Claims (1)
Number |
Date |
Country |
Kind |
2674/80 |
Jun 1980 |
DKX |
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RELATED APPLICATIONS
This application is a continuation-in-part of copending application Ser. No. 973,937, filed Dec. 28, 1978, for A METHOD FOR THERMAL DESTABILIZATION OF MICROBIAL RENNET, now U.S. Pat. No. 4,255,454.
US Referenced Citations (3)
Number |
Name |
Date |
Kind |
3723250 |
Aunstrup et al. |
Mar 1973 |
|
3886042 |
Blumberg et al. |
May 1975 |
|
4255454 |
Branner-Jorgensen |
Mar 1981 |
|
Foreign Referenced Citations (1)
Number |
Date |
Country |
2045772 |
Nov 1980 |
GBX |
Non-Patent Literature Citations (1)
Entry |
Vratsanos S. M., On the Mechanism of Enzyme Action, LXXI, Acylations of Trypsin in Organic Solvents, Archives of Biochemistry and Biophysics, vol. 90, 1960 (pp. 132-138). |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
973937 |
Dec 1978 |
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