Claims
- 1. An isolated Thermotoga delta prime subunit of a DNA polymerase III-type enzyme, the isolated delta prime subunit:
(i) comprising the amino acid sequence of SEQ ID NO: 148; or (ii) being encoded by a nucleic acid molecule hybridizing to the complement of SEQ ID NO: 147 under hybridization conditions comprising at most about 0.9M sodium citrate buffer at a temperature of at least about 37° C.
- 2. The isolated Thermotoga delta prime subunit according to claim 1 wherein the Thermotoga species is Thermotoga maritima.
- 3. The isolated Thermotoga delta prime subunit according to claim 2 wherein the delta prime subunit comprises the amino acid sequence of SEQ ID NO: 148.
- 4. The isolated Thermotoga delta prime subunit according to claim 1 wherein the delta prime subunit is encoded by a nucleic acid molecule that hybridizes to the complement of SEQ ID NO: 147 under hybridization conditions comprising at most about 0.9M sodium citrate buffer at a temperature of at least about 51° C.
- 5. The isolated Thermotoga delta prime subunit according to claim 1 wherein the delta prime subunit is purified.
- 6. A clamp loader complex comprising the Thermotoga delta prime subunit according to claim 1.
- 7. A DNA polymerase III-type enzyme complex comprising the claim loader according to claim 6.
- 8. A kit comprising:
a container that contains therein either a deoxynucleoside triphosphate or a dideoxynucleoside triphosphate; and a container that contains therein the DNA polymerase III-type enzyme complex according to claim 7.
Parent Case Info
[0001] The present application is a continuation of U.S. patent application Ser. No. 09/716,964, filed Nov. 21, 2000, which is a continuation-in-part of U.S. patent application Ser. No. 09/642,218, filed Aug. 18, 2000, as a continuation of U.S. patent application Ser. No. 09/057,416 filed Apr. 8, 1998, which claims the benefit of U.S. Provisional Patent Application Serial No. 60/043,202 filed Apr. 8, 1997, all of which are hereby incorporated by reference in their entirety.
Government Interests
[0002] The present invention was made with funding from National Institutes of Health Grant No. GM38839. The United States Government may have certain rights in this invention.
Provisional Applications (1)
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Date |
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60043202 |
Apr 1997 |
US |
Continuations (2)
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Parent |
09716964 |
Nov 2000 |
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Child |
10673098 |
Sep 2003 |
US |
Parent |
09057416 |
Apr 1998 |
US |
Child |
09642218 |
Aug 2000 |
US |
Continuation in Parts (1)
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09642218 |
Aug 2000 |
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09716964 |
Nov 2000 |
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