Medications for treatment of cancer, infection, thrombosis, and other diseases, are commonly very toxic and are therefore effective at the location of the tumor or infection, but toxic to normal tissues, resulting in significant side effects. Despite efforts to develop increasingly targeted and personalized therapeutics, system toxic side effects limit the use of specific medications and dosing of many medications for treatment of cancer, infection, and thrombolysis. One of the methods to limit toxicity common in medical practice for specific types of cancer is direct infusion of chemotherapy into the feeding artery supplying the tumor. This limits the systemic exposure of the toxic chemical and directs the chemotherapy to the region where treatment is needed.
Unfortunately, more than 50-80% of the injected drug is not trapped in the target organ and bypasses the tumor to general circulation. These toxic chemicals then enter the body and can cause side effects, which may include irreversible cardiac failure, fatigue, hair loss, bruising and bleeding, infection, anemia, nausea and vomiting, appetite changes, digestive disruptions, weight changes, cognitive abilities effect, mood changes, etc.
Industries routinely use absorption columns to remove pollutants from chemical streams. Embodiments here include porous absorbers for capturing excess chemotherapy drugs that do not become absorbed by the target tumor. The embodiments here introduce an absorber into the draining vein and then the absorber removes a significant fraction of the injected chemotherapy drugs. The absorber can also remove significant amounts of different types of drugs, such as anti-microbials, thrombolytic agents, toxins from bacterial infections, environmental toxins, or cells, may be captured, or bound, using specific chemical, physical biological, and any combinations thereof using different features of the three-dimensional, printed absorbers. In addition, the absorbers can capture/remove unwanted molecules/materials in the body which the natural systems in the body do not properly function to remove them, and result in chronic health issues. For example, lactic acid could be removed from the blood of patients with acute lactic acidosis.
The embodiments here also include porous materials for releasing drugs at the target location at a constant rate required for the condition of patients. The embodiments here also include a system to release the drug at the target upstream location and capture the drug at the downstream location. One should note that while the apparatus is referred to as an absorber, it also may act as a delivery system to release drugs, such as nano-particles or micro-particles of therapeutic compounds and other agents for use in health care. The term absorber encompasses both the release and absorption aspects of the embodiments.
While the discussion below focuses on chemotherapy drugs, the application of the absorbers to remove other harmful materials from the body should not be limited to this. The term ‘toxin’ as used here means any material that is toxic to the human body, even if it is used to treat tumors or other illnesses. The toxin is harmful to other parts of the body. The examples of toxins include candidate chemotherapeutics.
Candidate chemotherapeutics include, but are not limited to alkylating agents: bifunctional alkylators (Cyclophosphamide, Mechlorethamine, Chlorambucil, Melphalan), and monofunctional alkylators (Dacarbazine, Nitrosoureas, Temozolomide), (using DNA strands); anthracyclines: doxorubicin, daunorubicin, dipirubicin, iadrubicin, mitoxantrone, and valrubicin (using ion-exchange functional groups); cytoskeletal disruptors: pacilaxel, docetaxel, abraxane, and taxotere (using protein (whole protein or subunit of protein) based capture; Epothilones; Histone deacetylase inhibitors (HDAC): vorinostat, and romidepsin (using protein and/or DNA based capture); Inhibitors of topoisomerase I: irinotecan, and topotecan (using protein and/or DNA based capture); Inhibitors of topoisomerase II: etoposide, teniposide, and tafluposide (using protein and/or DNA based capture); Nucleotide analogs: hydroxyurea, anacitidine, capecitabine, cytarabind, and doxifluridine, etc. (using protein and/or DNA based capture); Peptide antibiotics: bleomycin, and actinomycin; Platinum-based agents: carboplatin, cisplatin, and oxaliplatin (using DNA strands); Retinoids; Vinca alkaloids and derivatives: vinblastine, vincristine, vindesine, and vinorelbine (using protein and/or DNA based capture).
The term ‘vessel’ as used here means any vessel into which the absorber is placed. Typically, this will be the vein that drains the body structure, such as an organ, but could be placed into other vessels as well. In addition, the absorbers can be located in organ, such as in fatty layers of organs, or can be designed to be deployed in any location of the body if necessary.
As used here, the term “lattice” is a structure that forms a unit cell of a repeating structure. In
Generally, the absorber consists of a cylinder having within it a scaffold or lattice of material. The shape of the absorber/releaser is dictated by the shape of the blood vessel at the target location of the patient. This can be determined for each individual patient by methods such as MRI and printed in accordance to the specifics of the patient. The scaffold or lattice has a coating or other surface modification that absorbs the toxin. Because it can be printed, the cylinder could have any circumference or length, customizable to the individual user's vessels.
The scaffold or lattice surfaces can be modified using different chemical reactions, such as polymerization, catalytic reactions, surface coating, etching, surface modification such as a dopamine coating, cross-linkage, etc., to introduce functional groups that can capture/bind to target toxins. Functional groups can be strong or weak cation exchange groups, strong or weak anion exchange groups, DNA strands, biological ligands, proteins, antibodies, enzymes, nano-particles, micro-particles, magnetic particles, etc. depending upon the target molecules. Magnetic particles are used for imaging with NMR and/or MRI. The removal of such magnetic particles after operation is important for safety.
In addition, the absorbing or releasing coating could be formed by many different kinds of polymers. For example, one possibility could consist of block copolymers wherein one of the blocks contains the active functional groups above, while the other causes the polymer to adhere to the scaffold. Other possibilities include random copolymers comprising functional and adhering monomers, and graft copolymers comprising functional and adhering monomers.
The surface of the absorbers can be designed to capture multiple drugs to be used with drug cocktails that are commonly used in cancer chemotherapy. The absorber surface can be printed or modified to have multiple layers of different materials to facilitate the capacity and rate of drug capture, and the capture the mixtures of drugs and target molecules. Absorbers can be prepared using elastomeric materials with controlled mechanical properties. The surface modification is selected based upon its ability to bond with the particular target molecules, either for release or capture. The target molecules, whether for release or capture, whether drugs or nano-particles or micro-particles, will be referred to here as “compounds.”
Absorbers with different designs and coatings can be deployed at the same time or in rapid succession to affect multiple toxins, drugs, or processes.
The system may incorporate the absorber and other related parts. The absorbers can incorporate guide wires, an introducing sheath, and/or other related devices, such as balloons for easier operation with less blood loss. The absorbers can be compressible and stretchable to fill the vessels of interest and for easier operation. The absorbers of different chemical formula and/or mechanical properties can be assembled in desired locations to optimize their binding/releasing abilities to target molecules, not interfere with the blood flow, and/or manipulate the blood flow to a desirable rate. Device design features including device shape and balloons can also be used to alter blood flow adjacent to the device to improve drug binding or release.
The dimensions, shape, and mechanical properties of the absorber can be carefully modified to manipulate the blood flow to a desirable rate, in terms of volumetric flow rate, blood flow residence time contacting the absorbers, circulating the blood flow in the absorbers, etc. Such dimensions, shape and mechanical properties of the absorbers can be determined by the size of vessels, location, and the blood flow rate in the desired location. The absorbers can be used as balloons or stents to construct the vessel or organs in which a structures is destroyed or collapsed, such as in an aneurism, and/or slow down the blood flow.
Regarding the incorporation of sheath and guide wires with the absorbers by using 3D printing, the sheath, guidewire, and/or other necessary parts for operation can be prepared by special chemical formula to make these parts function as the absorbers well. The inside and outside of sheath, guidewire, and/or other parts can be modified, such as by surface modification, to have special functional groups to bind/release target molecules for drug capture/drug release purposes. The whole system consisting of sheath, guidewire, and other parts, which may include catheters, connecting parts, etc., may be referred to here as “the absorber/releasing system.” Certain designs of porous scaffolds may be manufactured by more conventional polymer processing methods such as injection molding.
The following discussion focuses on chemotherapy with the understanding that this is merely for discussion purposes only. Cancer is becoming the leading cause of death in most westernized nations. Although there have been enormous efforts to develop more targeted and personalized cancer therapeutics, dosing of drugs in cancer chemotherapy is often limited by systemic toxic side effects. During intra-arterial chemotherapy infusion to a target organ, excess drug not trapped in the target organ passes through to the veins draining the organ, and then circulates to the rest of the body, causing toxicities in distant locations. Typically, more than 50-80% of the injected drug is not trapped in the target organ and bypasses the tumor to general circulation.
In the context of reducing the toxicity of chemotherapy, the embodiments present the development of a new biomedical device: an absorber that captures excess chemotherapeutic drug before it is released in the body. This absorber is temporarily deployed in the vein draining the organ undergoing intra-arterial chemotherapy infusion, and is removed after the infusion is completed.
Doxorubicin is a low-cost, highly effective agent frequently used in chemotherapy for several decades. Based on a linear dose response model, increasing the dose of doxorubicin linearly increases tumor cell death. This provides motivation for higher-dose doxorubicin therapy, but the side effects of high dose doxorubicin therapy include irreversible cardiac failure, which limits implementation of the high dose regimen. An established and highly effective agent like doxorubicin is a compelling first candidate for demonstrating the proposed drug capture approach.
For the absorber to work efficiently in the embodiments using doxorubicin in liver infusion chemotherapy, it must selectively bind the target drug within an hour or less. The structure of the absorber must be carefully designed and fabricated so as not to severely impair blood flow or cause thrombosis, although patients are usually anticoagulated during interventional radiology procedures limiting thrombosis. Custom-made absorbers must be used as individual patients have veins of different dimensions. The inventors have used 3D printing to fabricate the absorbers used in this study. Successful design, fabrication and deployment of the absorber has the potential to open a new route to help patients fight cancer.
Porous cylinders, shown in
Poly(ethylene glycol)-based polymers are widely used in biomedical engineering because of their biocompatibility and fouling resistance. Moreover, other relevant properties such as mechanical strength and water swelling of the PEG based polymers can be readily tuned by controlling the polymerization conditions. Optical micrographs of the 3D printed porous cylinders are shown in
The surface of the porous cylinders was coated with a poly(tert-butylstyrene)-b-poly(ethylene-co-propylene)-b-poly(styrene-co-styrene sulfonate)-b-poly(ethylene-co-propylene)-b-poly(tert-butylstyrene) (PtBS-PEP-PSS-PEP-PtBS) block polymer provided by Kraton Performance Polymers, Inc. (Houston, Tex.). The chemical structure of block copolymer is shown in
In one embodiment, the choice for the polymer coating was informed by previous studies where it was shown that polystyrenesulfonate chains demonstrated high capacity for binding with doxorubicin. It is likely that the PtBS and PEP blocks in the block copolymer are responsible for adhesion between the coating and 3D printed scaffold. The approach for coating the cylinders described here was arrived at after considerable trial and error. Small changes in the composition of either the block copolymer or the solvent result in unstable coating on the scaffolds.
In vivo experiments were performed with the coated 3D printed absorbers described above in three animal models (swine). The diameter of the absorbers (5 mm) was determined by the size of the introducer sheath, in one embodiment the sheath consisted of an 18 French or 6 mm diameter sheath that could be accommodated in the common femoral and common iliac veins of the swine. These are similar in diameter to the hepatic veins in an adult human.
The diameter of the introducer sheath is minimized to minimize blood loss during the operation. The length of the absorbers (30 mm) was chosen to match the length of the common iliac vein. The common iliac vein 42 was chosen to facilitate interpretation of experimental data and demonstrate proof-of-concept. Also, the diameter of the common iliac vein is approximately 10 mm, like the diameter of human hepatic veins near their confluence with the inferior vena cava where the absorbers will be placed for capturing excess drug draining the liver during hepatic intra-arterial chemotherapy infusion as shown in see
The absorbers were tested in the swine models undergoing chemo-infusion in the common iliac vein 42 of 50 mg of Doxorubicin over 10 min, corresponding to a typical dose used clinically in chemotherapy for intra-arterial treatment of hepatocellular carcinoma. Doxorubicin concentrations were monitored as a function of time using blood-sampling catheters at three different locations. Two locations, the pre-device 44 and post-device 46 sampling catheters, are depicted schematically in
X-ray fluoroscopy images of the absorbers in the common iliac vein obtained during one of the in vivo experiments are shown in
Results of two separate in vivo experiments are shown in
In this experiment, 69% of the doxorubicin is captured by the coated 3D printed absorbers. The images of the plasma from the centrifuged samples obtained from three sampling catheters during this experiment, shown in
The experiments suggest doxorubicin binds to the absorbers irreversibly; the inventors tried to release the doxorubicin by pumping the aqueous potassium chloride and ethanol mixture described above past the filters for one month. Analysis of the mixture showed negligible doxorubicin concentrations (less than 0.001 mg/ml). The images shown in
The in vivo drug capture experiments were repeated four times. The results of the other three experiments were like those reported in
The embodiments here include designed, built, and deployed porous absorbers for capturing chemotherapy drugs in vivo before they are released in the body to reduce systemic toxic side effects. The porosity was obtained by 3D printing of the lattice structure within the cylinders. The application of a polystyrenesulfonate coating on the absorber was essential for drug capture. The initial design enables the capture of 69% of the administered drug without noticeable adverse side effects.
Numerous approaches for using the platform have been developed to improve the efficacy of drug capture. Most simply, the number of absorber devices could be increased, increasing total surface area for drug biding. The lattice size could be decreased to enhance drug capture. Additional improvement in performance may be obtained by changing the chemical composition and thickness of the coating layer or by changing the lattice structure such as from cubic to hexagonal or by making a non-uniform lattice with larger pores in the front. The lattice may be of different geometries, they could be cube-like or hexagonal, of be quasi-periodic structures like a quasicrystal, or an aperiodic structure with different geometries at the front and back. The geometry of the lattice could change continuously either radially or axially with wider struts at some locations and narrower struts at other locations.
In future clinical trials one may use custom 3D printed elastomeric absorbers with patient-specific form factors that fit optimally in the vein(s) of the patient, as can be created from pre-procedure Computed Tomography (CT) or Magnetic Resonance Imaging (MRI) datasets.
Cylindrical porous absorbers for this study were prepared at Carbon, Inc., a 3D printing company located at Redwood City, Calif., USA. The prepolymer solution was prepared by adding 1 wt % initiators (i.e., 0.8 wt % of 2, 4, 6-Trimethylbenzoyl-diphenylphosphine oxide (TPO, Sigma Aldrich, USA) and 0.2 wt % of 2-Isopropylthioxanthone (ITX, Esstech, Inc., USA) and 0.23 wt % of carbon black pigment to poly(ethylene glycol) diacrylate (PEGDA, MW=250 g/mol, Sigma Aldrich, USA.) (see
The surface of the 3D printed porous cylinders was modified by coating a thin layer of sulfonated styrenic pentablock copolymers. The sulfonated styrenic pentablock copolymers (PtBS-PEP-PSS-PEP-PtBS) were synthesized via anionic polymerization and a subsequent post-polymerization sulfonation process, and detailed procedures have been described elsewhere. The sulfonation level (mol %) of the middle polystyrene (PS) block was controlled to a desired ion exchange capacity (IEC). In this study, the sulfonated pentablock copolymer of the IEC=2.0 meq/g (dry polymer) (sulfonation level=52 mol %) was used. The number average molecular weight of unsulfonated pentablock copolymer is approximately 78,000 g/mol (block mass fractions are PtBS:PEP:PSS=0.33:0.27:0.40), and the volume fraction of mid PSS block is 0.434.
The uncoated and coated absorbers were imaged using synchrotron hard X-ray microtomography at beamline 8.3.2. of the Advanced Light Source at Lawrence Berkeley National Laboratory. X-rays with energies ranging from 12-25 keV were generated by the synchrotron and illuminated the sample. The X-ray shadow transmitted through the sample was converted using a scintillator into visible light. This image was magnified by an optical microscope and converted into a digital image file. As the sample was rotated through 180° by a fraction of a degree, a total of 1,313˜2016 images were collected. These projection images were reconstructed using the program Xi-Cam to cross-sectional slice images, and subsequently stacked to generate 3D reconstructed images of the 397 cylinders.
3D printed absorbers were tested in vivo in three swine models (40-45 kg). The absorber was strung along a polytetrafluoroethylene (PTFE) coated nitinol guide wire (Glidewire®, Terumo Interventional Systems, Somerset, N.J., USA) for smooth and rapid movement through tortuous blood vessels; The guide wire went through the middle hole of the absorber, and two metallic fasteners on each end of the absorber were used to keep the absorber in place.
In vivo experiments were performed under compliance with the protocols of the Institutional Animal Care and Use Committee (IACUC) at the University of California, San Francisco (UCSF). Each animal was monitored with blood pressure, pulse oximetry, heart rate, and electrocardiogram while under general anesthesia with isoflurane. An 18 French (diameter=6 mm) introducer sheath placed into the common femoral vein was used to deliver the absorbers into the common iliac vein. Sampling and injection catheters were placed under fluoroscopy guidance at the spot of interest relative to the absorbers. Pre-device sampling catheter was introduced via the sheath to the left common iliac vein. Post-device sampling catheter was introduced through the internal jugular vein and was placed into the common iliac vein adjacent to the bifurcation of the vena cava. The distances between the catheters and absorbers were carefully adjusted to be consistent over a series of in vivo experiments. Prior to the start of the experiments, patency of the venous system was demonstrated using iodinated contrast injection (iohexol, Omnipaque-300, GE Healthcare, USA).
To simulate intra-arterial chemotherapy dosing, 50 mg of Doxorubicin (2 mg/ml, Doxorubicin 419 hydrochloride Injection, United States Pharmacopeia, Pfizer, New York, N.Y., USA) was injected into the common iliac vein via an infusion pump at a constant rate of 2.5 ml/min over 10 min. Blood aliquots of 2 ml at different times from the pre-device, post-device, and peripheral sampling locations were collected after 1.5 ml of blood was wasted to account for the volume within the catheter.
Doxorubicin concentrations in the blood aliquots were determined using fluorescence spectroscopy. Fluorescence measurements were made using a FlexStation 3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, Calif.) at a known emission wavelength of 550 nm upon excitation with a 480 nm laser. The doxorubicin concentration was calculated from the measured fluorescence at 550 nm using the calibration curve, which correlates fluorescence emission to doxorubicin concentration.
The coated and uncoated absorbers of Example 1 were used in additional in vivo experiments were performed on four pigs by deploying the multiple coated absorbers in the hepatic veins and Inferior Vena Cava (IVC) of the animals as depicted in
Doxorubicin concentrations in liver, heart, and kidneys were measured to evaluate the reduction of doxorubicin accumulation in major organs. Control experiments were performed on three pigs where no absorber was deployed but the pigs underwent the same intra-arterial infusion of doxorubicin (200 mg) as described above. For easier comparison, the doxorubicin concentrations in the organs of the control experiments were used to normalize the doxorubicin concentrations in those of the in vivo experiments where the coated absorbers were deployed.
The result of organ tissue analysis is shown in
The above discussion focused on removal of excess chemotherapy drugs in an infusion treatment. As mentioned previously, this is just one example of an application and is not intended to limit the scope of the claims. Other types of medications and substances may be removed using these absorbers. Further, the techniques and structure of the absorbers may be used to release localized medications or other therapeutic agents after insertion.
It will be appreciated that variants of the above-disclosed and other features and functions, or alternatives thereof, may be combined into many other different systems or applications. Various presently unforeseen or unanticipated alternatives, modifications, variations, or improvements therein may be subsequently made by those skilled in the art which are also intended to be encompassed by the following claims.
This application claims priority to U.S. Provisional Patent Application 62/664,508, filed Apr. 30, 2018, which is incorporated by reference here in its entirety.
This invention was made with government support under Contract/Grant Numbers CA194533 and EB012331 awarded by the National Institutes of Health and Contact/Grant Numbers DE-ACO2-05CH11231 and DE-ACO2-05CH11232 awarded by the Department of Energy. The government has certain rights in the invention.
Filing Document | Filing Date | Country | Kind |
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PCT/US19/29979 | 4/30/2019 | WO | 00 |
Number | Date | Country | |
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62664508 | Apr 2018 | US |