Claims
- 1. An implantable porous, biodegradable, three-dimensionally fixed matrix having shape memory comprising a network of water-insoluble mineralized biopolymer fibers, and a water-soluble binder which is rendered insoluble by cross-linking.
- 2. An implantable, porous, biodegradable, three-dimensionally fixed matrix for bone growth having shape memory which maintains its porosity for a period greater than about fourteen days after implant into a physiological environment in which said bone growth is required, comprising insoluble mineralized biopolymer fibers and a water-soluble binder which is rendered insoluble by cross-linking.
- 3. A matrix according to claim 1 or 2 wherein said matrix is compressible from its initial size, shape and porosity and returns from a compressed state without substantial loss of said fibers or binder.
- 4. A matrix according to claim 1 or 2 further comprising an additive.
- 5. A matrix according to claim 4 wherein said additive comprises a drug.
- 6. A matrix according to claim 5 wherein said drug comprises an antibiotic.
- 7. A matrix according to claim 5 further comprising a growth factor.
- 8. A matrix according to claim 5 further comprising marrow.
- 9. A matrix according to any of claims 1-8 wherein said binder is selected from the group consisting of soluble collagen, gelatin, polylactic acid, polyglycolic acid, copolymers of lactic and glycolic acid, polycaprolactone, carboxymethylcellulose, cellulose esters, dextrose, dextran, chitosan, hyaluronic acid, ficol, chondroitin sulfate, polyvinyl alcohol, polyacrylic acid, polypropylene glycol, polyethylene glycol, water soluble polyacrylates and water soluble polymethacrylates.
- 10. A matrix according to claim 1 or 2 wherein said biopolymer comprises fibrillar collagen.
- 11. A matrix according to claim 1 or 2 wherein said mineral comprises hydroxyapatite.
- 12. A matrix according to claim 2 wherein said mineral is released into said physiological environment during replacement with bone in a time-release profile which maintains said porosity for said period of time.
- 13. A matrix according to claim 5 wherein said collagen and immobilized calcium phosphate are in the form of mineralized collagen containing about 30-95% by weight of collagen.
- 14. A matrix according to claim 1 or 2 further comprising marrow cells.
- 15. A matrix according to claim 1 or 2 further comprising autogenous bone.
- 16. A method of making a porous, biodegradable, three-dimensionally fixed matrix having shape memory comprising the steps of:
(a) forming a dispersion comprising mineralized biopolymer fibers and a water-soluble binder; (b) lyophilizing said dispersion to form a porous matrix; and (c) cross-linking said porous matrix.
- 17. A method according to claim 16 wherein said biopolymer comprises fibrillar collagen.
- 18. A method according to claim 16 wherein said binder is selected from the group consisting of soluble collagen, gelatin, polylactic acid, polyglycolic acid, copolymers of lactic and glycolic acid, polycaprolactone, carboxymethylcellulose, cellulose esters, dextrose, dextran, chitosan, hyaluronic acid, ficol, chondroitin sulfate, polyvinyl alcohol, polyacrylic acid, polypropylene glycol, polyethylene glycol, water soluble polyacrylates and water soluble polymethacrylates.
- 19. A method according to claim 16 wherein said binder comprises soluble collagen.
- 20. A method according to claim 16 wherein the mineral in said mineralized biopolymer fibers comprises hydroxyapatite.
- 21. A method according to claim 16 wherein said dispersion further comprises leachable particles and said step (b) further comprises leaching said particles to form said matrix.
- 22. A method according to claim 16 wherein said dispersion further comprises sublimable particles and said step (b) further subliming said particles to form said matrix.
- 23. A method according to claim 16 wherein said mineralized biopolymer fiber comprise mineralized collagen fibrils containing about 30-95% by weight of collagen.
- 24. A method of repairing fibrous tissue or cartilage comprising the step of introducing to a target site of repair a composition comprising are effective amount of a porous, biodegradable, three-dimensionally fixed matrix having shape memory comprising insoluble mineralized biopolymer fibers and a water-soluble binder which is rendered insoluble by cross-linking, sufficient to promote new fibrous tissue or cartilage formation.
- 25. A method of repairing fibrous tissue, cartilage or bone comprising the steps of:
(a) introducing a delivery vehicle to a target site of repair, said vehicle containing a porous, biodegradable, three-dimensionally fixed matrix having shape memory comprising insoluble mineralized biopolymer fibers and a water-soluble binder which is rendered insoluble by cross-linking in a compressed state relative to the initial size, shape and porosity of said matrix; and (b) releasing said matrix from said delivery vehicle whereby said matrix returns to said initial size, shape and porosity.
- 26. A method according to claim 24 or 25 wherein said matrix returns to said initial size, shape and porosity without loss of a substantial amount of said fibers and binder.
- 27. A method according to claim 24 or 25 wherein said matrix further comprising an additive.
- 28. A method according to claim 27 wherein said additive comprises a drug.
- 29. A method according to claim 28 wherein said drug comprises an antibiotic.
- 30. A method according to claim 27 wherein said additive comprises a growth factor.
- 31. A method according to claim 24 or 25 wherein said matrix further comprises marrow.
- 32. A method according to claim 24 or 25 wherein said binder is selected from the group consisting of soluble collagen, gelatin, polylactic acid, polyglycolic acid, copolymers of lactic and glycolic acid, polycaprolactone, carboxymethylcellulose, cellulose esters, dextrose, dextran, chitosan, hyaluronic acid, ficol, chondroitin sulfate, polyvinyl alcohol, polyacrylic acid, polypropylene glycol, polyethylene glycol, water soluble polyacrylates and water soluble polymethacrylates.
- 33. A method according to claim 24 or 25 wherein said biopolymer comprises fibrillar collagen.
- 34. A method according to claim 24 or 25 wherein the mineral of said mineralized biopolymer fibers comprises hydroxyapatite.
- 35. A method according to claim 24 or 25 wherein said matrix comprises collagen and immobilized calcium phosphate in the form of mineralized collagen containing about 30-95% by weight of collagen.
- 36. A method according to claim 24 or 25 wherein said matrix further comprises autogenous bone.
- 37. A method according to claim 32 wherein said binder comprises soluble collagen.
- 38. A method of growing bone comprises the step of introducing a target site to an effective amount of a composition comprising an implantable, porous, biodegradable, three-dimensionally fixed matrix having shape memory which maintains its porosity for a period of greater than about fourteen days after implant into a physiological environment in which said bone growth is required, said matrix comprising insoluble mineralized biopolymer fibers and a water-soluble binder which is rendered insoluble by cross-linking.
- 39. A method according to claim 38 wherein said matrix further comprises an additive.
- 40. A method according to claim 39 wherein said additive comprises a drug.
- 41. A method according to claim 40 wherein said drug comprises an antibiotic.
- 42. A method according to claim 39 wherein said additive comprises a growth factor.
- 43. A method according to claim 38 wherein said matrix further comprises marrow.
- 44. A method according to claim 38 wherein said binder is selected from the group consisting of soluble collagen, gelatin, polylactic acid, polyglycolic acid, copolymers of lactic and glycolic acid, polycaprolactone, carboxymethylcellulose, cellulose esters, dextrose, dextran, chitosan, hyaluronic acid, ficol, chondroitin sulfate, polyvinyl alcohol, polyacrylic acid, polypropylene glycol, polyethylene glycol, water soluble polyacrylates and water soluble polymethacrylates.
- 45. A method according to claim 38 wherein said biopolymer comprises fibrillar collagen.
- 46. A method according to claim 38 wherein the mineral of said mineralized biopolymer fibers comprises hydroxyapatite.
- 47. A method according to claim 38 wherein the mineral of said mineralized biopolymer fibers is released into said physiological environment during replacement with bone in a time-release profile which maintains said porosity for said period of time.
- 48. A method according to claim 38 wherein said matrix comprises collagen and immobilized calcium phosphate in the form of mineralized collagen containing about 30-95% by weight of collagen.
- 49. A method according to claim 38 wherein said matrix further comprises autogenous bone.
- 50. A method according to claim 44 wherein said binder comprises soluble collagen.
Parent Case Info
[0001] The priority is claimed of U.S. provisional application Serial No. 60/005,523, filed Oct. 16, 1995. This is a continuation-in-part of U.S. Ser. No. 09/782,794, filed Feb. 13, 2001, which is a continuation of U.S. Ser. No. 09/110,726, filed Jul. 7, 1998, now U.S. Pat. No. 6,187,047; which is a divisional of U.S. Ser. No. 08/633,554, filed Apr. 17, 1996, now U.S. Pat. No. 5,776,193, all of which are incorporated by reference herein.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60005523 |
Oct 1995 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
08633554 |
Apr 1996 |
US |
Child |
10086225 |
Feb 2002 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09110726 |
Jul 1998 |
US |
Child |
10086225 |
Feb 2002 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09782794 |
Feb 2001 |
US |
Child |
10086225 |
Feb 2002 |
US |