Patent Application
20240247248
The present disclosure claims benefit of Chinese Patent Application No. 202310090475.3, filed on Jan. 19, 2023, entitled “To-Be-Tested Object Preparation Apparatus and Nucleic Acid Testing Integrated Machine with To-Be-Tested Object Preparation Apparatus”, the contents of which are hereby incorporated by reference in its entirety.
The disclosure relates to a technical field of biological detection, in particular to a to-be-tested object preparation apparatus and a nucleic acid testing integrated machine with the to-be-tested object preparation apparatus.
At present, nucleic acid testing is widely used in clinical diagnosis, agricultural monitoring, food safety, and other fields. Polymerase Chain Reaction (PCR) is the most commonly used method for nucleic acid testing. The general principle of PCR detection is a process of in vitro replication of daughter strand Deoxyribo Nucleic Acid (DNA) complementary to parent strand DNA with the parent strand DNA as a template and a specific primer as an extension starting point under the catalysis of DNA polymerase.
In the related art, when extracting and replicating the parent strand DNA, a nucleic acid testing integrated machine requires the cooperation of a plurality of modules therein, so as to extract the parent strand DNA from a sample by using an extraction reagent, and then transfer the parent strand DNA to an amplification reagent for replication.
However, the cooperation of the plurality of modules easily leads to the problem that the occupied area of the nucleic acid testing integrated machine is too large.
The disclosure provides a to-be-tested object extraction apparatus and a nucleic acid testing integrated machine with the to-be-tested object preparation apparatus, so as to solve the problem that the occupied area of the nucleic acid testing integrated machine in the related technology is too large.
According to one aspect of the disclosure, a to-be-tested object extraction apparatus is provided. The to-be-tested object extraction apparatus includes: a to-be-tested object loading area, provided with a to-be-tested object loading portion, the to-be-tested object loading portion being configured to store and process a to-be-tested object tube; a sample reaction area, provided with a sample reaction portion, the sample reaction portion being configured to extract nucleic acid from a to-be-tested object; a sample adding area, provided with a sample adding portion, the sample adding portion being configured to transfer the to-be-tested object from the to-be-tested object loading portion to a first reaction vessel and add the extracted nucleic acid in the sample reaction portion to a second reaction vessel; and a grabbing area, provided with a grabbing portion, the grabbing portion being configured to move the first reaction vessel after adding the to-be-tested object from the sample adding portion to the sample reaction portion and move the first reaction vessel after extracting the nucleic acid from the to-be-tested object from the sample reaction portion to the sample adding portion. The to-be-tested object loading area, the sample adding area, and the grabbing area are arranged around the outside of the sample reaction area, and the grabbing area is located on the same side of the to-be-tested object loading area, the sample reaction area, and the sample adding area.
In some embodiments, the to-be-tested object extraction apparatus further includes a to-be-tested object tube transfer piece, a reaction vessel transfer piece, and an extraction mechanism transfer piece. The to-be-tested object tube transfer piece is movably arranged between the to-be-tested object loading area and the sample adding area to move the to-be-tested object tube in the to-be-tested object loading area to the sample adding area, the reaction vessel transfer piece is movably arranged between the sample adding area and the grabbing area to move the first reaction vessel and the second reaction vessel in the sample adding area between the sample adding area and the grabbing area, and the extraction mechanism transfer piece is movably arranged between the sample reaction area and the grabbing area to move the first reaction vessel between the sample reaction area and the grabbing area.
In some embodiments, the to-be-tested object tube transfer piece is movably arranged between the to-be-tested object loading area and the sample adding area in a first horizontal direction, the reaction vessel transfer piece is movably arranged between the sample adding area and the grabbing area in a second horizontal direction, the extraction mechanism transfer piece is movably arranged between the sample reaction area and the grabbing area in the second horizontal direction, and the first horizontal direction is perpendicular to the second horizontal direction.
In some embodiments, the reaction vessel transfer piece includes a first reaction vessel transfer piece and a second reaction vessel transfer piece which are arranged in parallel. The first reaction vessel transfer piece and the second reaction vessel transfer piece are movably arranged between the sample adding area and the grabbing area in the second horizontal direction. The sample adding portion includes a pipetting needle. The pipetting needle is movably arranged above the first reaction vessel transfer piece and the second reaction vessel transfer piece, the first reaction vessel transfer piece is provided with a sample adding position located in the sample adding area, and when the first reaction vessel transfer piece is located in the sample adding position, the pipetting needle is able to move to above the first reaction vessel transfer piece to fill the to-be-tested object into the first reaction vessel. The second reaction vessel transfer piece is provided with a nucleic acid adding position located in the sample adding area and flush with the first reaction vessel transfer piece, and when the first reaction vessel transfer piece is located in the sample adding position and the second reaction vessel transfer piece is located in the nucleic acid adding position, the pipetting needle is able to move to above the first reaction vessel transfer piece to suck the extracted nucleic acid and move to above the second reaction vessel transfer piece to transfer the extracted nucleic acid to the second reaction vessel.
In some embodiments, the to-be-tested object tube transfer piece is provided with a first working position located in the sample adding area, and when the to-be-tested object tube transfer piece is located in the first working position, the pipetting needle is able to move to above the to-be-tested object tube transfer piece to suck the to-be-tested object.
In some embodiments, the to-be-tested object loading portion includes a to-be-tested object tube gripper and a to-be-tested object bin configured to store the to-be-tested object tube. The to-be-tested object tube gripper is movably arranged above the to-be-tested object bin and the to-be-tested object tube transfer piece, the to-be-tested object tube transfer piece is further provided with a second working position located in the to-be-tested object loading area, and when the to-be-tested object tube transfer piece is located in the second working position, the to-be-tested object tube gripper is able to move the to-be-tested object tube from the to-be-tested object bin to the to-be-tested object tube transfer piece. The grabbing portion includes a reaction vessel gripper movably arranged in the first horizontal direction and a vertical direction. The extraction mechanism transfer piece is provided with a receiving position located in the grabbing area and an extraction position located in the sample reaction area, the reaction vessel transfer piece is provided with a first transfer position located in the grabbing area, and when the reaction vessel transfer piece is located in the first transfer position and the extraction mechanism transfer piece is located in the receiving position, the reaction vessel gripper is able to grab the first reaction vessel to move the first reaction vessel between the reaction vessel transfer piece and the extraction mechanism transfer piece.
In some embodiments, the sample adding portion further includes a consumable storage bin configured to carry a pipette tip. The pipetting needle is able to move to above the consumable storage bin to load the pipette tip.
In some embodiments, the grabbing area is further provided with a film sealing mechanism configured to encapsulate the second reaction vessel, the reaction vessel transfer piece is further provided with a second transfer position located in the grabbing area, and when the reaction vessel transfer piece is located in the second transfer position, the reaction vessel gripper is able to move to above the reaction vessel transfer piece to grab the second reaction vessel loaded with the nucleic acid and move to above the film sealing mechanism to release the second reaction vessel to the film sealing mechanism.
In some embodiments, the film sealing mechanism includes a hot pressing piece and a film sealing mechanism transfer piece. The film sealing mechanism transfer piece is movably arranged below the hot pressing piece in the second horizontal direction, the film sealing mechanism transfer piece is provided with a cover body carrying part configured to place a cover body and a second reaction vessel carrying part configured to place the second reaction vessel, the reaction vessel gripper is able to release the second reaction vessel to the second reaction vessel carrying part, the reaction vessel gripper is provided with a grabbing position located above the film sealing mechanism transfer piece, and when the reaction vessel gripper is located in the grabbing position, the film sealing mechanism transfer piece is provided with a separation position where the cover body carrying part moves to below the reaction vessel gripper, so that the reaction vessel gripper grabs the cover body, a release position where the second reaction vessel carrying part moves to below the reaction vessel gripper, so that the reaction vessel gripper releases the cover body the second reaction vessel and an encapsulation position where the second reaction vessel carrying part moves to below the hot pressing piece.
In some embodiments, the grabbing area is further provided with a waste liquid station located below the reaction vessel gripper. The waste liquid station includes a waste liquid rack, a pallet, and a waste liquid recycling piece. The liquid waste recycling piece is movably arranged on the liquid waste rack in the vertical direction and the second horizontal direction, when the reaction vessel transfer piece is located in the first transfer position, the reaction vessel gripper is able to move to above the first reaction vessel transfer piece to grab the first reaction vessel and move to above the pallet to release the first reaction vessel to the pallet, and the waste liquid recycling piece is able to move to above the pallet to recycle a magnetic rod sleeve and waste liquid.
In some embodiments, the waste liquid station further includes a support plate, a push rod, and a recycling bin. The recycling bin is located below the waste liquid rack, the pallet is vertically movably arranged on the waste liquid rack, the push rod is movably arranged on the support plate in the second horizontal direction, the support plate is provided with an avoidance opening configured to avoid the pallet, the pallet is able to move to below the support plate to remain the first reaction vessel on the support plate, and the push rod is able to push the first reaction vessel to recycle the first reaction vessel to the recycling bin.
According to another aspect of the disclosure, a nucleic acid testing integrated machine is provided. The nucleic acid testing integrated machine includes a reagent preparation apparatus, a to-be-tested object extraction apparatus, and an amplification apparatus. The reagent preparation apparatus is configured to prepare an extraction reagent and an amplification reagent, the amplification apparatus is configured to perform analyte detection determination on a determination mixture to analyze a to-be-tested object, and the to-be-tested object extraction apparatus is the above provided to-be-tested object extraction apparatus.
In some embodiments, the reagent preparation apparatus, the to-be-tested object extraction apparatus, and the amplification apparatus are isolated from each other, the reagent preparation apparatus and the amplification apparatus are respectively located on both sides of the to-be-tested object extraction apparatus, a first channel is arranged between the reagent preparation apparatus and the to-be-tested object extraction apparatus, a second channel is arranged between the to-be-tested object extraction apparatus and the amplification apparatus, and the first channel and the second channel have an on state and an off state. And/or, the nucleic acid testing integrated machine further includes a ferry mechanism arranged between the reagent preparation device and the to-be-tested object extraction apparatus. The ferry mechanism is able to move a first reaction vessel configured to prepare the extraction reagent and a second reaction vessel configured to prepare the amplification reagent to the to-be-tested object extraction apparatus through the first channel, and a grabbing portion of the to-be-tested object extraction apparatus is able to move the first reaction vessel to a first reaction vessel placement position of the to-be-tested object extraction apparatus and move the second reaction vessel to a second reaction vessel placement position of the to-be-tested object extraction apparatus.
By applying the technical solutions of the disclosure, the to-be-tested object extraction apparatus includes the to-be-tested object loading area, the sample reaction area, the sample adding area, and the grabbing area, the to-be-tested object loading area is provided with the to-be-tested object loading portion to load and process the to-be-tested object tube for containing the to-be-tested object, the sample adding area is provided with the sample adding portion to transfer the to-be-tested object from the to-be-tested object loading portion to the first reaction vessel, the grabbing area is provided with the grabbing portion to move the first reaction vessel after adding the to-be-tested object from the sample adding portion to the sample reaction portion of the sample reaction area, the nucleic acid is extracted from the to-be-tested object by using the sample reaction portion, and then the grabbing portion moves the first reaction vessel after extracting the nucleic acid from the sample reaction portion to the sample adding portion, so as to add the extracted nucleic acid in the sample reaction portion to the second reaction vessel, thereby replicating the nucleic acid. Because the to-be-tested object loading area, the sample adding area, and the grabbing area are arranged around the outside of the sample reaction area, and the grabbing area is located on the same side of the to-be-tested object loading area, the sample reaction area, and the sample adding area. Therefore, the arrangement between modules of the to-be-tested object extraction apparatus is more compact, and the arrangement of the nucleic acid testing integrated machine is also more compact, so that the occupied area of the nucleic acid testing integrated machine is reduced.
The accompanying drawings of the specification, which constitute a part of the disclosure, are intended to provide a further understanding of the disclosure, and the exemplary embodiments of the disclosure and the description thereof are intended to explain the disclosure and do not constitute an undue limitation on the disclosure. In the accompanying drawings:
Herein, the above accompanying drawings include the following reference signs:
The technical solutions in the embodiments of the disclosure will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the disclosure. It is apparent that the described embodiments are only a part of the embodiments of the disclosure, and not all of them. The following description of at least one exemplary embodiment is only illustrative, and in no way serves as any limitation on the disclosure or application or use thereof. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the disclosure without creative efforts are within the scope of protection of the disclosure.
As shown in
By applying the technical solutions of the disclosure, the to-be-tested object extraction apparatus includes the to-be-tested object loading area 10, the sample reaction area 20, the sample adding area 30, and the grabbing area 40, the to-be-tested object loading area 10 is provided with the to-be-tested object loading portion 11 to load and process the to-be-tested object tube for containing the to-be-tested object, the sample adding area 30 is provided with the sample adding portion 31 to transfer the to-be-tested object from the to-be-tested object loading portion 11 to the first reaction vessel, the grabbing area 40 is provided with the grabbing portion 41 to move the first reaction vessel after adding the to-be-tested object from the sample adding portion 31 to the sample reaction portion 21 of the sample reaction area 20, the nucleic acid is extracted from the to-be-tested object by using the sample reaction portion 21, and then the grabbing portion 41 moves the first reaction vessel after extracting the nucleic acid from the sample reaction portion 21 to the sample adding portion 31, so as to add the extracted nucleic acid in the sample reaction portion 21 to the second reaction vessel, thereby replicating the nucleic acid. Because the to-be-tested object loading area 10, the sample adding area 30, and the grabbing area 40 are arranged around the outside of the sample reaction area 20, and the grabbing area 40 is located on the same side of the to-be-tested object loading area 10, the sample reaction area 20, and the sample adding area 30. Therefore, the arrangement between modules of the to-be-tested object extraction apparatus is more compact, and the arrangement of the nucleic acid testing integrated machine is also more compact, so that the occupied area of the nucleic acid testing integrated machine is reduced.
In the embodiment, the sample reaction portion 21 includes an extraction mechanism. The nucleic acid is extracted from a sample in the first reaction vessel by using the extraction mechanism. The extraction mechanism may be a mechanism structure in the prior art, such as the structure in the Chinese Patent Application No. 202222368037.2.
It is to be noted that a transfer mechanism is arranged between areas of the to-be-tested object extraction apparatus to realize the transfer of consumables and the to-be-tested object between the areas.
As shown in
As shown in
The reaction vessel transfer piece is movably arranged between the sample adding area 30 and the grabbing area 40 in a second horizontal direction, the extraction mechanism transfer piece 80 is movably arranged between the sample reaction area 20 and the grabbing area 40 in the second horizontal direction by a second driving mechanism, and the first horizontal direction is perpendicular to the second horizontal direction. The extraction mechanism transfer piece 80 may be a second base with a second carrying part for carrying the first reaction vessel. The second driving mechanism may be a belt mechanism or a cylinder mechanism.
The to-be-tested object tube transfer piece 50 moves in the first horizontal direction, and the reaction vessel transfer piece and the extraction mechanism transfer piece 80 move in the second horizontal direction, which shortens moving paths of the to-be-tested object tube transfer piece 50, the reaction vessel transfer piece, and the extraction mechanism transfer piece 80, and then reduces the space occupation, so as to reduce the occupied area of the nucleic acid integrated machine.
As shown in
The sample adding portion 31 includes a pipetting needle 311. The pipetting needle 311 is movably arranged above the first reaction vessel transfer piece 60 and the second reaction vessel transfer piece 70, the first reaction vessel transfer piece 60 is provided with a sample adding position located in the sample adding area 30, and when the first reaction vessel transfer piece 60 is located in the sample adding position, the pipetting needle 311 is able to move to above the first reaction vessel transfer piece 60 to fill the to-be-tested object into the first reaction vessel. The pipetting needle 311 is able to suck the to-be-tested object in the to-be-tested object tube and release it into the first reaction vessel on the first reaction vessel transfer piece 60, and the first reaction vessel transfer piece 60 moves the first reaction vessel filled with the to-be-tested object from the sample adding area 30 to the grabbing area 40.
In the embodiment, the to-be-tested object extraction apparatus is provided with a frame body, the to-be-tested object loading portion 11, the sample reaction portion 21, the sample adding portion 31, and the grabbing portion 41 are arranged in the frame body, and the sample adding portion 31 further includes a sample arm. The sample arm is movably arranged on the frame body in the horizontal direction, and the pipetting needle 311 is movably arranged on the sample arm in the vertical direction, so that the three-axis movement of the pipetting needle 311 is realized.
Specifically, the second reaction vessel transfer piece 70 is provided with a nucleic acid adding position located in the sample adding area 30 and flush with the first reaction vessel transfer piece 60, and when the first reaction vessel transfer piece 60 is located in the sample adding position and the second reaction vessel transfer piece 70 is located in the nucleic acid adding position, the pipetting needle 311 is able to move to above the first reaction vessel transfer piece 60 to suck the extracted nucleic acid and move to above the second reaction vessel transfer piece 70 to transfer the extracted nucleic acid to the second reaction vessel. The extracted nucleic acid in the sample reaction portion 21 is transferred to the second reaction vessel located in the second reaction vessel transfer piece 70 by using the pipetting needle 311, and then the nucleic acid is replicated by using an amplification reagent in the second reaction vessel.
The to-be-tested object tube transfer piece 50 is provided with a first working position located in the sample adding area 30, and when the to-be-tested object tube transfer piece 50 is located in the first working position, the pipetting needle 311 is able to move to above the to-be-tested object tube transfer piece 50 to suck the to-be-tested object. The to-be-tested object tube transfer piece 50 moves the to-be-tested object tube from the to-be-tested object loading area 10 to the sample adding area 30, and the pipetting needle 311 sucks the to-be-tested object in the to-be-tested object tube on the to-be-tested object tube transfer piece 50 and fill it to the first reaction vessel on the first reaction vessel transfer piece 60.
In the embodiment, through the arrangement of the reaction vessel transfer piece, a sample adding function of filling the to-be-tested object from the to-be-tested object tube to the first reaction vessel and a nucleic acid transfer function of filling the extracted nucleic acid from the first reaction vessel to the second reaction vessel are realized in the sample adding area 30, which is conducive to the simplification of the structure of the to-be-tested object extraction apparatus.
As shown in
In the embodiment, the to-be-tested object tube gripper 111 realizes the three-axis movement on the frame body, so as to move the to-be-tested object tube from the to-be-tested object bin 112 to the to-be-tested object tube transfer piece 50. The frame body is provided with a transverse guide rail and a longitudinal guide rail. The to-be-tested object tube gripper 111 includes a gripper frame and a clamping jaw vertically movably arranged on the gripper frame. The gripper frame is able to move in the extension direction of the transverse guide rail and the longitudinal guide rail to realize the movement of the gripper frame in the horizontal plane, and then realize the three-axis movement of the clamping jaw.
It is to be noted that the to-be-tested object includes samples, quality control products, calibration products, etc.
As shown in
In the embodiment, the first reaction vessel transfer piece 60 is located in the first transfer position, and the reaction vessel gripper 411 grabs the first reaction vessel to move the first reaction vessel between the first reaction vessel transfer piece 60 and the extraction mechanism transfer piece 80.
In the embodiment, the transmission of consumables between the areas is realized through the coordination of the movement of the transfer piece between the areas in the horizontal direction and the movement of the gripper in the area in the horizontal direction and the vertical direction, and the areas operate independently and work in parallel, the compact structure is realized, and the work efficiency is ensured.
Specifically, the to-be-tested object loading portion 11 further includes a cover opening piece 12. The to-be-tested object tube transfer piece 50 is further provided with a third working position. When the to-be-tested object tube transfer piece 50 is located in the third working position, the to-be-tested object tube transfer piece 50 is located below the cover opening piece 12, and the third working position is located between the first working position and the second working position. A cover of the to-be-tested object tube located on the to-be-tested object tube transfer piece 50 is opened by using the cover opening piece 12, so that the sample adding portion 31 is able to suck the to-be-tested object in the to-be-tested object tube.
In the embodiment, the frame body is provided with a vessel gripper guide rail extending in the first horizontal direction, and the reaction vessel gripper 411 includes a vessel gripper frame and an orifice plate clamping jaw. The vessel gripper frame is movably arranged on the vessel gripper guide rail in the extension direction of the vessel gripper guide rail, and the orifice plate clamping jaw is vertically movably arranged on the vessel gripper frame. Through the adoption of the above structure, the two-axis movement of the reaction vessel gripper 411 is realized.
Specifically, both the first reaction vessel transfer piece 60 and the extraction mechanism transfer piece 80 are able to move to the grabbing area 40, and then the reaction vessel gripper 411 is able to move the first reaction vessel filled with the to-be-tested object from the first reaction vessel transfer piece 60 to the extraction mechanism transfer piece 80. Then, the extraction mechanism transfer piece 80 moves the first reaction vessel to the extraction position, so that the nucleic acid is extracted by using the extraction mechanism.
As shown in
Specifically, the pipetting needle 311 needs to move to above the consumable storage bin 312 to load the pipette tip before the pipetting needle 311 sucks the to-be-tested object in the to-be-tested object tube. Then, the to-be-tested object is sucked by using the pipette tip. The process of sucking the nucleic acid is similar to the above, and will not be elaborated herein.
In the embodiment, the grabbing area 40 is further provided with a film sealing mechanism 43, the reaction vessel transfer piece is further provided with a second transfer position located in the grabbing area 40, and when the reaction vessel transfer piece is located in the second transfer position, the reaction vessel gripper 411 is able to move to above the reaction vessel transfer piece to grab the second reaction vessel loaded with the nucleic acid and move to above the film sealing mechanism 43 to release the second reaction vessel to the film sealing mechanism 43. The second reaction vessel loaded with the nucleic acid is transferred to the film sealing mechanism 43 by using the reaction vessel gripper 411, and the second reaction vessel loaded with the nucleic acid is encapsulated by using the film sealing mechanism 43.
Specifically, the second reaction vessel transfer piece 70 is located in the second transfer position, and the reaction vessel gripper 411 is able to move to above the second reaction vessel transfer piece 70 to grab the second reaction vessel loaded with the nucleic acid.
As shown in
The reaction vessel gripper 411 moves the reaction second vessel loaded with the nucleic acid to the film sealing mechanism transfer piece 432, and moves the cover body from the cover body carrying part 433 to the second reaction vessel, and then the encapsulation of the cover body and the second reaction vessel is completed by using the hot pressing piece 431.
As shown in
In the embodiment, an upper end of the waste liquid recycling piece 423 is provided with a protruding portion, the magnetic rod sleeve is recycled by using the protruding portion, a lower end of the waste liquid recycling piece 423 is provided with a waste liquid needle, and the waste liquid is sucked by using the waste liquid needle.
As shown in
Specifically, after the first reaction vessel after extracting the nucleic acid is transferred to the pallet 422 by using the reaction vessel gripper 411, the waste liquid recycling piece 423 moves to above the first reaction vessel to recycle the magnetic rod sleeve and the waste liquid. After the magnetic rod sleeve and the waste liquid are recycled, the pallet 422 vertically moves and penetrates through the avoidance opening, so that the first reaction vessel remains on the support plate 424, and then the push rod 425 is able to move the first reaction vessel on the support plate 424 to the recycling bin.
In the embodiment, when extracting the nucleic acid in the to-be-tested object, the to-be-tested object tube gripper 111 moves to above the to-be-tested object bin 112 to grab the to-be-tested object tube to the to-be-tested object tube transfer piece 50 located in the second working position, the to-be-tested object tube is transferred to the third working position below the cover opening piece 12 by the to-be-tested object tube transfer piece 50, the cover is opened by using the cover opening piece 12, and after the cover is opened, the to-be-tested object tube moves to the first working position in the sample adding area 30 by the to-be-tested object tube transfer piece 50, and the pipetting needle 311 moves to above the to-be-tested object tube transfer piece 50 to suck the to-be-tested object. Then, the pipetting needle 311 moves to the sample adding area 30, and moves to above the first reaction vessel transfer piece 60 in the sample adding position, so as to release the to-be-tested object into the first reaction vessel of the first reaction vessel transfer piece 60 by using the pipetting needle 311. Then, the first reaction vessel transfer piece 60 moves to the first transfer position located in the grabbing area 40, and the reaction vessel gripper 411 grabs the first reaction vessel on the first reaction vessel transfer piece 60. Then, the reaction vessel gripper 411 moves to above the extraction mechanism transfer piece 80 located in the receiving position, so as to release the first reaction vessel to the extraction mechanism transfer piece 80, and move the extraction mechanism transfer piece 80 to the extraction position in the extraction mechanism, so that the nucleic acid in the to-be-tested object is extracted by using the extraction mechanism. After the nucleic acid is extracted, the extraction mechanism transfer piece 80 moves from the extraction position located in the sample reaction area to the receiving position located in the grabbing area 40, and the reaction vessel gripper 411 grabs the first reaction vessel and releases it to the first reaction vessel transfer piece 60 located in the first transfer position. Then, the first reaction vessel transfer piece 60 moves to the sample adding position located in the sample adding area 30, the extracted nucleic acid in the first reaction vessel is sucked by using the pipetting needle 311, and then the pipetting needle 311 moves to above the second reaction vessel transfer piece 70 located in the nucleic acid adding position, so as to release the nucleic acid into the second reaction vessel. Then, the second reaction vessel transfer piece 70 moves to the second transfer position located in the grabbing area 40, the reaction vessel gripper 411 grabs the second reaction vessel, and the reaction vessel gripper 411 moves to the grabbing position located above the film sealing mechanism transfer piece 432 and release the second reaction vessel to the second reaction vessel carrying part 434. The film sealing mechanism transfer piece 432 moves to the separation position, the reaction vessel gripper 411 grabs the cover body, then the film sealing mechanism transfer piece 432 moves to the release position to release the cover body to the second reaction vessel, and then the film sealing mechanism transfer piece 432 moves to the encapsulation position to encapsulate the cover body and the second reaction vessel by using the hot pressing piece 431.
The first horizontal direction refers to an X-axis direction in
Another embodiment of the disclosure provides a nucleic acid testing integrated machine. The nucleic acid testing integrated machine includes a reagent preparation apparatus, a to-be-tested object extraction apparatus, and an amplification apparatus. The reagent preparation apparatus is configured to prepare an extraction reagent and an amplification reagent, the amplification apparatus is configured to perform analyte detection determination on a determination mixture to analyze a to-be-tested object, and the to-be-tested object extraction apparatus is the above provided to-be-tested object extraction apparatus. Through the adoption of the nucleic acid testing integrated machine, because the to-be-tested object loading area 10, the sample adding area 30, and the grabbing area 40 are arranged around the outside of the sample reaction area 20, the grabbing area 40 is located on the same side of the to-be-tested object loading area 10, the sample reaction area 20, and the sample adding area 30. Therefore, the arrangement between modules of the to-be-tested object extraction apparatus is more compact, and the arrangement of the nucleic acid testing integrated machine is also more compact.
In the embodiment, the reagent preparation apparatus, the to-be-tested object extraction apparatus, and the amplification apparatus are isolated from each other, the reagent preparation apparatus and the amplification apparatus are respectively located on both sides of the to-be-tested object extraction apparatus, a first channel is arranged between the reagent preparation apparatus and the to-be-tested object extraction apparatus, a second channel is arranged between the to-be-tested object extraction apparatus and the amplification apparatus, and the first channel and the second channel have an on state and an off state. Through the adoption of the above structure, isolation between modules of the apparatus is realized by closing the first channel and the second channel, thereby avoiding cross contamination. The transmission of consumables between the modules of the apparatus is realized by conducting the first channel and the second channel.
The nucleic acid testing integrated machine further includes a ferry mechanism arranged between the reagent preparation device and the to-be-tested object extraction apparatus. The ferry mechanism is able to move a first reaction vessel configured to prepare the extraction reagent and a second reaction vessel configured to prepare the amplification reagent to the to-be-tested object extraction apparatus through the first channel, and a grabbing portion 41 of the to-be-tested object extraction apparatus is able to move the first reaction vessel to a first reaction vessel placement position of the to-be-tested object extraction apparatus and move the second reaction vessel to a second reaction vessel placement position of the to-be-tested object extraction apparatus. The ferry mechanism moves the first reaction vessel that completes the preparation of the extraction reagent and the second reaction vessel that completes the preparation of the amplification reagent in the reagent preparation apparatus to the to-be-tested object extraction apparatus for the extraction of the to-be-tested object.
It is to be noted that the terms used herein is only for the purpose of describing the specific implementation modes and is not intended to limit the exemplary implementation modes of the disclosure. As used herein, the singular form is also intended to include the plural form unless otherwise expressly stated in the context, and it should also be understood that when the terms “contain” and/or “include” are used in the specification, they indicate the presence of features, steps, operations, devices, components and/or combinations thereof.
Unless otherwise specified, the relative arrangement, numerical expressions and numerical values of parts and steps set forth in these embodiments do not limit the scope of the disclosure. Also, it should be understood that, for ease of description, the dimensions of various parts shown in the accompanying drawings are not drawn to scale. Techniques, methods, and devices known to those of ordinary skill in the related fields may not be discussed in detail, but should be regarded as part of the specification under appropriate circumstances. In all examples shown and discussed herein, any specific value should be interpreted as exemplary only and not as a limitation. Thus, other examples of the exemplary embodiments may have different values. It is to be noted that: similar numbers and letters refer to similar items in the following accompanying drawings, and thus, once an item is defined in one accompanying drawing, it does not require further discussion in subsequent accompanying drawings.
In the description of the disclosure, it is to be understood that the orientations or positional relationships indicated by the orientation words “front, rear, upper, down, left and right”, “transverse, longitudinal, vertical and horizontal”, “top and bottom”, etc. are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the disclosure and simplifying the description. Unless stated to the contrary, these orientation words do not indicate or imply that the apparatus or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as limiting the scope of protection of the disclosure. The orientation words “inside and outside” refer to inside and outside relative to the outline of each part itself.
For ease of description, spatially relative terms, such as “over”, “above”, “on the surface”, “upper”, etc., may be used herein to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figures. It should be understood that the spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figures. For example, if the devices in the accompanying drawings are inverted, those described as “above other devices or structures” or “over other devices or structures” will then be positioned as “below other devices or structures” or “under other devices or structures”. Thus, the exemplary term “above” may include both “above” and “below” orientations. The device may also be positioned in various other ways (rotated 90 degrees or at other orientations) and the spatially relative descriptions used herein are interpreted accordingly.
Furthermore, it is to be noted that the use of the words “first”, “second” and the like to define parts is only for the convenience of distinguishing the corresponding parts, unless otherwise stated, the words have no special meaning, and therefore cannot be construed as limiting the scope of protection of the disclosure.
The above is only the preferred embodiments of the disclosure, and is not intended to limit the disclosure, and for those of ordinary skill in the art, various modifications and changes may be made to the disclosure. Any modifications, equivalent substitutions, improvements, etc. within the spirit and scope of the disclosure shall be included in the scope of protection of the disclosure.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202310090475.3 | Jan 2023 | CN | national |
