TOOLS FOR STUDYING EUKARYOTIC CELL MEMBRANE TRAFFICKING

Information

  • Research Project
  • 2024240
  • ApplicationId
    2024240
  • Core Project Number
    R43GM055687
  • Full Project Number
    1R43GM055687-01
  • Serial Number
    55687
  • FOA Number
  • Sub Project Id
  • Project Start Date
    2/1/1997 - 27 years ago
  • Project End Date
    1/31/1998 - 26 years ago
  • Program Officer Name
  • Budget Start Date
    2/1/1997 - 27 years ago
  • Budget End Date
    1/31/1998 - 26 years ago
  • Fiscal Year
    1997
  • Support Year
    1
  • Suffix
  • Award Notice Date
    1/17/1997 - 27 years ago
Organizations

TOOLS FOR STUDYING EUKARYOTIC CELL MEMBRANE TRAFFICKING

DESCRIPTION (Adapted from applicant's abstract): Clostridial neurotoxins (NTs) have been extremely valuable for elucidating the mechanisms of neurotransmitter secretion from nerve cells since eight distinct NTs were found to act as specific proteases on only three neurosynaptic proteins: synaptobrevin, syntaxin and SNAP-25. However, a novel perspective has recently emerged from studies of NT action in non-neural cells. Once believed to be neural-specific, NTs introduced into the cytosol of non- neural cells by bypassing neural-specific cell-surface receptors are found to inhibit aspects of membrane trafficking. While surprising, these findings are understandable since a number of non-neural homologs of NT substrate proteins have been identified. The goals of this project are to develop a safe, efficient and general method for inducibly expressing Clostridial NT light chains in nonneural cells, and to employ this method to characterize the range of NT action on membrane trafficking in eukaryotic cells. The work proposed here will lead to the identification of specific membrane trafficking steps that are interrupted by NTs and to the characterization of NT substrates involved in these steps. Additional mutagenesis experiments are proposed to generate novel NT variants that have acquired a new substrate range. This work will also provide general tools for cell biological studies of commercial potential for investigating membrane trafficking in eukaryotic cells.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R43
  • Administering IC
    GM
  • Application Type
    1
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    ZRG3
  • Study Section Name
  • Organization Name
    PROMEGA CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    MADISON
  • Organization State
    WI
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    537115399
  • Organization District
    UNITED STATES