Patent Application
20240082136
The invention relates to topical compositions comprising an amino acid, which is a nitric oxide donor; and methods of topical treatment using such compositions.
Nitric oxide (“NO”) is a small gaseous molecule also known as nitrogen monoxide with the chemical formula NO, which has been shown to be a very important signaling molecule in living organisms including the human body, and particularly the skin and its underlying tissues.
In the skin, NO enhances cutaneous microcirculation, it is involved in skin pigmentation through ultraviolet induced melanogenesis, it has been reported to promote wound healing by cellular proliferation and angiogenesis and to have antimicrobial properties against micro-organisms. NO also plays an important role in T-cell mediated diseases of the skin, and it has both pro and anti-apoptotic properties depending on its concentration, cell type, and availability of other substrates.
Thus, it would be beneficial to administer NO into the skin; however, it is impractical to apply NO in its gas state to the skin, in a way that will facilitate such biological effects or impart a therapeutic effect, and thus, the use of certain NO-donors has been proposed.
While NO possesses beneficial effects, as summarized above, it also has the potential to impart notable cutaneous side effects, due to its potent pro-inflammatory properties. NO is known to mediate cutaneous oedema and inflammation and may contribute to impaired skin barrier function.
Thus, there remains an unmet need for administering effective amounts of NO into the skin, while suppressing its untoward pro-inflammatory properties.
An aspect of the invention pertains to a topical composition for application to the skin, comprising an amino acid, which is a nitric oxide donor.
In one or more embodiments, the amino acid is selected from the group consisting of l-arginine and l-citrulline; and cosmetically or pharmaceutically acceptable salts, isomers, analogs and derivatives thereof.
In one or more embodiments, the amino acid is l-arginine, and its concentration is selected from the group of between about 6%-about 25%, from about 6%-8%, from about 8%-10%, from about 10%-14%, from about 14%-18%, and from about 18%-25%.
In one or more embodiments, the amino acid is i-citrulline and its concentration is selected from the group of between about 4%-about 20%, from about 4%-6%, from about 6%-10%, from about 10%-14% and from about 14%-20%.
In one or more embodiments, the composition further comprising nicotinamide or salts, isomers, analogs and derivatives thereof.
In one or more embodiments, the concentration of nicotinamide is between about 1%-about 2%, or from about 2%-4%, or from about 4%-6%, or from about 6%-8%, or from about 8%-10%.
In one or more embodiments, the ratio between the arginine and nicotinamide in the topical composition is between about 2:1 and 8:1, or between about 2:1 and 4:1, or between about 4:1 and 6:1, or between about 6:1 and 8:1.
In one or more embodiments, the ratio between the citrulline and nicotinamide in the topical composition is between about 2:1 and 8:1, or between about 2:1 and 4:1, or between about 4:1 and 6:1, or between about 6:1 and 8:1.
In one or more embodiments, upon topical application to the skin the composition exerts a tolerable tingling effect.
Another aspect of the invention pertains to a method of treating, alleviating or preventing a skin condition, comprising administering topically to the skin an effective amount of a composition as herein disclosed.
Yet another aspect pertains to a composition as herein disclosed for use in treating, alleviating or preventing a skin condition, wherein the composition is applied topically to the skin of a subject in need thereof.
In one or more embodiments, the skin condition is associated with skin ageing.
In one or more embodiments, the skin condition includes at least one of the following: light-induced skin ageing, increased transepidermal water loss, skin dryness, fine lines, wrinkles, photoaging, hyperpigmentation and skin discoloration.
In one or more embodiments, the application is conducted in concurrence with the dermal administration of a neurotoxin.
In one or more embodiments, the concentration of arginine in the composition is selected from the group of between about 6%-about 25%, from about 6%-8%, from about 8%-10%, from about 10%-14%, from about 14%-18%, and from about 18%-25%.
In one or more embodiments, the concentration of nicotinamide in the composition is between about 1%-about 2%, or from about 2%-4%, or from about 4%-6%, or from about 6%-8%, or from about 8%-10%.
In one or more embodiments, the ratio between the arginine and nicotinamide in the topical composition is between about 2:1 and 8:1, or between about 2:1 and 4:1, or between about 4:1 and 6:1, or between about 6:1 and 8:1.
In one or more embodiments, the application of the composition increases the supplies of oxygen and nutrients to the skin tissues.
In one or more embodiments, the application of the composition augments the effect of the neurotoxin and/or prolongs the duration of the effect of the neurotoxin.
In one or more embodiments, the application of the composition is conducted prior to administration of the neurotoxin, or on a continuous basis following the administration of the neurotoxin.
In one or more embodiments, the application of the composition alleviates the inflammation and or the atrophy that is induced following administration of the neurotoxin.
In one or more embodiments, the composition is in a form selected from the group consisting of a liquid, a solution, an emulsion, a lotion, a cream, a gel, a foam, a lipstick, a mask and a serum.
Although the following detailed description contains many specifics for the purpose of illustration, a person of ordinary skill in the art will appreciate that many variations and alterations to the following details can be made and are considered to be included herein. Accordingly, the following embodiments are set forth without any loss of generality to, and without imposing limitations upon, any claims set forth. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.
The invention relates to a topical composition comprising an amino acid, which is NO-donor.
The invention further relates to a method of treating, alleviating or preventing a dermatological, cosmetic or mucosal condition, comprising administering topically to a subject having said disorder a therapeutically effective amount of any of the compositions described herein.
Amino Acids which are Nitric Oxide Donors
Nitric oxide, having the molecular formula NO, is a very small molecule that, upon release from a composition, can migrate fast and reach its target site. Nitric oxide is a remarkably versatile biological messenger. The chemical properties of NO are crucial in defining its biological roles, both as a transcellular signal in the cardiovascular and nervous systems and as a cytotoxic antipathogenic agent released during an inflammatory response. Among other properties, NO is known to be an antibiotic. The term “antibiotic” as used herein includes, but is not limited to, a destructive or inhibitory effect on the growth of bacteria; or the capacity to inhibit the growth of or to destroy bacteria. Likewise, NO is known to be effective in eradicating fungi, yeast, molds and viruses. NO can further have anti-inflammatory effects and skin revitalizing effects and it is also known to enhance wound healing. It can further be used to treat various dermatoses and keratoses.
Upon penetration into and through the skin, NO can cause peripheral vasodilation, otherwise described as enhanced blood flow in the skin, which facilitates improved provision of oxygen and nutrients into the subcutaneous tissues, the dermis and the epidermis.
l-arginine (arginine, l-arginine and L-arginine are used herein interchangeably) is a naturally occurring amino acid. There are three major pathways for l-arginine metabolism: l-arginine is metabolised to 1-ornithine by arginase, to agmatine by arginine decarboxylase and to NO and citrulline by NO synthase (NOS), through the following mechanism:
We surprisingly found that the topical application of a composition, comprising a high concentration of l-arginine can be effective in the treatment or prevention of skin condition that can be treated or alleviated by elevated concentrations of NO.
In an additional embodiment, the amino acid is and l-citrulline (citrulline, l-citrulline and L-citrulline are used herein interchangeably).
Due to the above-mentioned attributes of NO, it can be beneficial, when it is present in the skin in biologically-effective concentrations, it can have beneficial effects on skin disease or condition. For example, NO has antimicrobial properties, i.e., antibacterial, antifungal and antiviral, properties and thus it has the potential to be used in the treatment of infections of the skin, including but not limited to:
The symptoms of a skin infection vary depending on the type. Common symptoms include redness of the skin and a rash. People with infection may also experience other symptoms, such as itching, pain, and tenderness. Signs of a severe infection include pus, blisters, skin sloughing, breakdown, dark, necrotic-appearing skin, or skin that becomes discolored and painful.
Acne is a disease that involves an infection by the anaerobic bacterial species Cutibacterium acnes (also named Propionibacterium acnes); and rosacea is also associated with microbial infections (e.g., by Bacillus oleronius).
Additional skin conditions that may involve microbial infection include, in a non-limiting fashion, atopic dermatitis, eczema and psoriasis.
Hence, the topical administration of a composition comprising a sufficient amount of an l-arginine has the potential of treating any of the above skin infections and/or their respective symptoms.
Likewise, the topical administration of an l-arginine has the potential of treating certain symptoms of the skin, which are considered “cosmetic”.
Dry skin is the result of impairment of the water-loss barrier of the skin, which is typical to damaged skin, such as in the event of atopic dermatitis, as well as ageing skin. Topical treatment of patients with atopic dermatitis with an arginine hydrochloride has been shown to significantly increase urea in the stratum corneum as well as an increase in skin moisture, and a consequent improvement of the clinical symptoms of dry skin. One of the most disturbing consequences of dry skin is the formation of wrinkles, which can be abundant across the whole-body surface, with special emphasis on the face, throat and chest, and thus, preventing the impairment of skin barrier function can further impede the formation of wrinkles. Furthermore, as NO induces peripheral blood flow, it can mobilize increased levels of oxygen and nutrients to skin layers, thereby facilitating reconstruction of the tissue and reduction of wrinkle appearance.
As noted above, high concentrations of NO in the skin can cause heating of the skin and inflammation, associated with skin redness (erythema), swelling (oedema) and a burning sensation. Such are considered untoward effects, which may prohibit the use of products by people in need, despite the potential benefits.
Any salts, isomers, analogs and derivatives, that convert into l-arginine through a chemical or an enzymatic process is useful in accordance with the present invention.
Concentrations of l-Arginine in the Composition
The concentration of l-arginine in the topical composition of the present invention is determined by the amount that is sufficient to elevate the level of NO in the skin, upon topical application of the composition. Since measuring NO levels in humans is impractical, such elevation can be observed by beneficial effects of the composition, upon application to the skin, in the treatment or prevention of skin condition that can be treated or alleviated by elevated concentrations of NO.
In an embodiment of the present invention, the concentration of l-arginine in the composition is between about 6%-about 25%; and in other embodiments it can be from about 6%-8%, or from about 8%-10%, or from about 10%-14%, or from about 14%-18%, or from about 18%-25%.
In a specific embodiment, the concentration is about 10%.
In the case of l-citrulline, in certain embodiments, the concentration of citrulline is between about 4%-about 20%; and in other embodiments it can be from about 4%-6%, or from about 6%-10%, or from about 10%-14% or from about 14%-20%.
Nicotinamide
We further surprisingly discovered that combining l-arginine with nicotinamide provides a synergistic benefit in the treatment of the skin.
Nicotinamide is known to exert a plurality of beneficial effects when administered to the skin, including, without limitation, the correction of light-induced skin ageing (it helps protect from UV and blue light damage, reduces the appearance of lines and wrinkles, improves the elasticity of the skin, rebalances uneven skin tone and reduces discoloration; it helps restore skin barrier function and reduces trans-epidermal water loss, which is the underlying factor for induces skin ageing.
Nicotinamide has also been demonstrated to be effective in the treatment and/or prevention of skin diseases, including but not limited to treating acne, rosacea, atopic dermatitis, pruritus (itch), melasma, blistering disorders, photoaging and photo immunosuppression; and it has also been shown to have a chemopreventative role in nonmelanoma skin cancer.
Any salts, isomers, analogs and derivatives of nicotinamide that have a counter-NO property are suitable in the context of the present invention.
Concentrations of Nicotinamide in the Composition
In an embodiment, the concentration of nicotinamide in the composition is between about 1%-about 2%; and in other embodiments it can be from about 2%-4%, or from about 4%-6%, or from about 6%-8%, or from about 8%-10%.
In an observatory trial, when subjects applied topically a composition comprising 10% arginine and 2% nicotinamide, several subjects sensed very slight warmth of the skin or a slight tingling sensation, while the rest did not have any such sensation. Of note, the very slight warmth of the skin or the slight tingling sensation were not considered prohibitive by the subjects, who said that “it feels like the product works”.
Thus, in certain embodiments, the ratio between l-arginine and nicotinamide in the topical composition is between about 2:1 and 8:1; and in other embodiments it can be between about 2:1 and 4:1, or between about 4:1 and 6:1, or between about 6:1 and 8:1.
Synergistic Effect
As noted above, both l-arginine and nicotinamide possess beneficial effects on the skin upon topical application; and in several cases, they attack the same condition via separate mechanisms. A non-limiting example is acne, where NO, which is produced from l-arginine is antimicrobial and can eradicate the acne pathogen P. acnes, while nicotinamide exerts anti-inflammatory and anti-comedogenic effects on the same acne lesions. The result can be more effective treatment of acne, which can be asseses by a higher proportion of acne lesions that are cured, and/or a shorter duration to the onset of the therapeutic effect.
Additional Therapeutic Agent
Several conditions involve a combination of etiological factors, some of which are affected by NO; and other etiological factors that require an additional therapeutic modality. For example, psoriasis may be treated by NO, as well as a steroid drug, and therefore combined treatment would be beneficial. Likewise, acne, which involves a microbial infection, excessive keratin production, excessive sebum production and inflammation, can benefit from treatment with a combination NO, and an additional therapeutic agent, selected from the group consisting of an anti-inflammatory agent, an antibiotic agent, a sebostatic agent and a keratolytic agent. Hence, in many cases, the inclusion of an additional therapeutic agent in the composition contributes to the clinical activity of NO.
Suitable additional therapeutic agents include but are not limited to active herbal extracts, herbal oils, herbal tinctures, acaricides, age spot and keratose removing agents, allergen, analgesics, antiacne agents, antiallergic agents, antiaging agents, anti-bacterials, antibiotics, antiburn agents, anticancer agents, antidandruff agents, antidepressants, anti-dermatitis agents, anti-edemics, antihistamines, anti-helminths, anti-hyperkeratolyte agents, anti-inflammatory agents, anti-irritants, anti-lipemics, antimicrobials, antimycotics, antiproliferative agents, antioxidants, anti-wrinkle agents, anti-pruritics, anti-psoriatic agents, anti-rosacea agents anti-seborrheic agents, antiseptic, anti-swelling agents, antiviral agents, anti-yeast agents, astringents, topical cardiovascular agents, chemotherapeutic agents, corticosteroids, dicarboxylic acids, disinfectants, fungicides, hair growth regulators, hormones, hydroxy acids, immunosuppressants, immunoregulating agents, insecticides, insect repellents, keratolytic agents, lactams, metals, local anesthetics, metal oxides, mitocides, neuropeptides, non-steroidal anti-inflammatory agents, oxidizing agents, pediculicides, photodynamic therapy agents, retinoids, scabicides, self-tanning agents, skin whitening agents, vasoconstrictors, vasodilators, vitamins, vitamin D derivatives, wound healing agents and wart removers. As is known to one skilled in the art, in some instances a specific active agent may have more than one activity, function or effect.
Forms of the Composition
The composition of the resent invention comprises a vehicle that contains l-arginine and that may further contain nicotinamide. While the composition may have various rheological characteristics, the following non-limiting examples of forms of the composition are provided for demonstration purposes.
In an embodiment, the composition is liquid. A liquid composition is flowable.
In an embodiment, the composition is an aqueous liquid, wherein l-arginine and nicotinamide are either in solution or in suspension.
In additional embodiments, the composition is a semi-solid. In certain embodiments the composition has a viscosity of more than about 5,000 Cps and it can have a viscosity selected from the group of: between about 5,000 Cps and about 100,000 Cps; between about 5,000 Cps and about 20,000 Cps; between about 20,000 Cps and about 60,000 Cps; and between about 60,000 Cps and about 100,000 Cps.
In an embodiment, the composition is a gel. The viscosity of the gel can be attained using customary polymeric or gelling agents. Exemplary polymeric or gelling agents include, in a non-limiting manner, naturally-occurring polymeric materials, such as locust bean gum, sodium alginate, sodium caseinate, egg albumin, gelatin agar, carrageenan gum, sodium alginate, xanthan gum, quince seed extract, tragacanth gum, guar gum, cationic guars, hydroxypropyl guar gum, starch, amine-bearing polymers such as chitosan; acidic polymers obtainable from natural sources, such as alginic acid and hyaluronic acid; chemically modified starches and the like, carboxyvinyl polymers, polyvinyl pyrrolidone, polyvinyl alcohol, polyacrylic acid polymers, polymethacrylic acid polymers, polyvinyl acetate polymers, polyvinyl chloride polymers, polyvinylidene chloride polymers and the like.
Additional exemplary polymeric agents include semi-synthetic polymeric materials such as cellulose ethers, such as methylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, hydroxyethyl cellulose, hydroxy propylmethyl cellulose, methylhydroxyethylcellulose, methylhydroxypropylcellulose, hydroxyethylcarboxymethylcellulose, carboxymethyl cellulose, carboxymethylcellulose carboxymethylhydroxyethylcellulose, and cationic celluloses. Polyethylene glycol, having molecular weight of 1000 or more (e.g., PEG 1,000, PEG 4,000, PEG 6,000 and PEG 10,000) also have gelling capacity and while they are considered herein as “secondary polar solvents”, as detailed herein, they are also considered polymeric agents.
Mixtures of the above polymeric agents are contemplated.
In an embodiment, the composition is an aqueous gel, i.e., a gel that contains water, wherein 1-arginine and nicotinamide are either in solution or in suspension.
Yet, in further embodiments, the composition is an emulsion, or micro-emulsion, or a nano-emulsion, which includes an aqueous phase and an organic carrier phase. Examples of topical dosage forms that comprise an emulsion are creams, lotions and emulsion-based sprays and foams.
Yet, in an additional embodiment, In the past, lipsticks have mainly focused on bringing decorative benefits (color shade, gloss) to the lips. Our lipsticks provide functional benefits such as rejuvenation, increasing the natural vitality and to provide nutrients and therapeutic agents.
The agents will also slow down/eliminate the enzymatic degradation of the injected Hyaluronic acid and other lips plumping agents.
In contrast to creams (see forms and compositions), lipsticks comprise pigments (colorants), oils and waxes. Many oils can be used in lipsticks, such as castor oil, mineral oils, and hydrogenated vegetable oils. The oils viscosity ranges from liquid to near wax-like, and they play a role of dispersant for colorants as well as cohesion enhancer in lipsticks. Typical oil concentrations range from 6 to 10%.
The most used waxes in lipsticks are beeswax and Carnauba wax. Waxes are used to increase the viscosity of a lipstick and balance the effects of oils and esters. Waxes are harder ingredients, and they raise the melting point of a formulation. This control in the melting temperature of the lipstick also controls the payoff of a lipstick, which is the amount of product transferred from the lipstick to the lips. Typical wax concentrations range from 8 to 18%.
Finally, polymers may be added to impart film-forming properties to lipsticks as well as to assist the formed film cohesion. Another benefit of polymers is the enhancement of wear-resistance. Usually, high-MW polymers are used for film adhesion and flexibility to follow the movements of the lips while lower-MW branched polymers can create a three-dimensional local network inside the film and traps colorant. Examples of polymers: acrylate/C12-22 alkylmethacrylate copolymers
The organic carrier is selected from a hydrophobic organic carrier (also termed herein “hydrophobic carrier”), an emollient and mixtures thereof.
In one or more embodiments, the hydrophobic carrier is an oil, such as mineral oil. According to one or more embodiments, hydrophobic carriers are oils originating from plant, marine or animal sources. By way of example, the plant oil may be olive oil, corn oil, soybean oil, canola oil, cottonseed oil, coconut oil, sesame oil, sunflower oil, borage seed oil, syzigium aromaticum oil, hempseed oil, herring oil, cod-liver oil, salmon oil, flaxseed oil, wheat germ oil, evening primrose oils or mixtures thereof, in any proportion.
Suitable hydrophobic carriers also include polyunsaturated oils that contain for example omega-3 and omega-6 fatty acids. Examples of such polyunsaturated fatty acids are linoleic and linolenic acid, gamma-linoleic acid (GLA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Such unsaturated fatty acids are known for their skin-conditioning effect, which may contribute to the therapeutic benefit of NO.
In the context of the present invention, oils that possess therapeutically-beneficial properties are termed “therapeutically active oil”.
Silicone oils may also be used. Suitable silicone oils include non-volatile silicones, such as polyalkyl siloxanes, polyaryl siloxanes, polyalkylaryl siloxanes and polyether siloxane copolymers, polydimethylsiloxanes (dimethicones) and poly(dimethylsiloxane)-(diphenyl-siloxane) copolymers. These are chosen from cyclic or linear polydimethylsiloxanes containing from about 3 to about 9, preferably from about 4 to about 5, silicon atoms. Volatile silicones such as cyclomethicones can also be used. Silicone oils are also considered therapeutically active oil, due to their barrier retaining and protective properties.
The organic carrier may contain a mixture of two or more of the above hydrophobic carriers in any proportion.
A further class of organic carrier includes “emollients” that have a softening or soothing effect, especially when applied to body areas, such as the skin and mucosal surfaces. Emollients are not necessarily hydrophobic. Examples of suitable emollients include hexyleneglycol, propylene glycol, isostearic acid derivatives, isopropyl palmitate, isopropyl isostearate, diisopropyl adipate, diisopropyl dimerate, maleated soybean oil, octyl palmitate, cetyl lactate, cetyl ricinoleate, tocopheryl acetate, acetylated lanolin alcohol, cetyl acetate, phenyl trimethicone, glyceryl oleate, tocopheryl linoleate, wheat germ glycerides, arachidyl propionate, myristyl lactate, decyl oleate, propylene glycol ricinoleate, isopropyl lanolate, pentaerythrityl tetrastearate, neopentylglycol dicaprylate/dicaprate, isononyl isononanoate, isotridecyl isononanoate, myristyl myristate, triisocetyl citrate, octyl dodecanol, sucrose esters of fatty acids, octyl hydroxystearate and mixtures thereof.
Surface-active agents (also termed “surfactants”) include any agent linking oil and water in the composition, in the form of emulsion. A surfactant's hydrophilic/lipophilic balance (HLB) describes the emulsifier's affinity toward water or oil. The HLB scale ranges from 1 (totally lipophilic) to 20 (totally hydrophilic), with 10 representing an equal balance of both characteristics. Lipophilic emulsifiers form water-in-oil (w/o) emulsions; hydrophilic surfactants form oil-in-water (o/w) emulsions. The HLB of a blend of two emulsifiers equals the weight fraction of emulsifier A times its HLB value plus the weight fraction of emulsifier B times its HLB value (weighted average).
According to one or more embodiments of the present invention, the surface-active agent has a hydrophilic lipophilic balance (HLB) between about 9 and about 14, which is the required HLB (the HLB required to stabilize an O/W emulsion of a given oil) of hydrophobic carriers or oils. Thus, in one or more embodiments, the composition contains a single surface-active agent having an HLB value between about 9 and 14, and in one or more embodiments, the composition contains more than one surface active agent and the weighted average of their HLB values is between about 9 and about 14. Yet, in other embodiments, when a water in oil emulsion is desirable, the composition contains one or more surface active agents, having an HLB value between about 2 and about 9.
The surface-active agent is selected from anionic, cationic, nonionic, zwitterionic, amphoteric and ampholytic surfactants, as well as mixtures of these surfactants. Such surfactants are well known to those skilled in the therapeutic and cosmetic formulation art. Nonlimiting examples of possible surfactants include polysorbates, such as polyoxyethylene (20) sorbitan monostearate (Tween 60) and poly(oxyethylene) (20) sorbitan monooleate (Tween 80); poly(oxyethylene) (POE) fatty acid esters, such as Myrj 45, Myrj 49, Myrj 52 and Myrj 59; poly(oxyethylene) alkylyl ethers, such as poly(oxyethylene) cetyl ether, poly(oxyethylene) palmityl ether, polyethylene oxide hexadecyl ether, polyethylene glycol cetyl ether, brij 38, brij 52, brij 56 and brij W1; sucrose esters, partial esters of sorbitol and its anhydrides, such as sorbitan monolaurate and sorbitan monolaurate; mono or diglycerides, isoceteth-20, sodium methyl cocoyl taurate, sodium methyl oleoyl taurate, sodium lauryl sulfate, triethanolamine lauryl sulfate and betaines.
In an embodiment, the composition of the present invention is a serum.
In an embodiment, the composition of the present invention is a foam.
In an embodiment, the composition of the present invention is attached to a mask, to be applied to the skin for an occlusive treatment; and to be removed following an extended period of application.
Treatment/Therapy
The terms “therapy” and “treatment” as used herein interchangeably, cover any treatment of a disease or disorder or a cosmetic condition, and includes, for example:
Fields of Pharmaceutical applications
By including l-arginine and optionally nicotinamide the composition of the present invention is useful in treating a patient having any one of a variety of dermatological disorders (also termed “dermatoses”), such as classified, in a non-limiting exemplary manner, according to the following groups: dermatitis, including contact dermatitis, atopic dermatitis, seborrheic dermatitis, nummular dermatitis, chronic dermatitis of the hands and feet, generalized exfoliative dermatitis, stasis dermatitis; lichen simplex chronicus; diaper rash; bacterial infections, including cellulitis, acute lymphangitis, lymphadenitis, erysipelas, cutaneous abscesses, necrotizing subcutaneous infections, staphylococcal scalded skin syndrome, folliculitis, furuncles, hidradenitis suppurativa, carbuncles, paronychial infections, erythrasma; fungal infections, including dermatophyte infections, yeast Infections; parasitic infections, including scabies, pediculosis, creeping eruption; viral infections; disorders of hair follicles and sebaceous glands, including acne, rosacea, perioral dermatitis, hypertrichosis (hirsutism), alopecia, male pattern baldness, alopecia areata, alopecia universalis and alopecia totalis; pseudofolliculitis barbae, keratinous cyst; scaling papular diseases, including psoriasis, pityriasis rosea, lichen planus, pityriasis rubra pilaris; benign tumors, including moles, dysplastic nevi, skin tags, lipomas, angiomas, pyogenic granuloma, seborrheic keratoses, dermatofibroma, keratoacanthoma, keloid; malignant tumors including basal cell carcinoma, squamous cell carcinoma, melanoma, paget's disease of the nipples, kaposi's sarcoma; reactions to sunlight, including sunburn, chronic effects of sunlight, photosensitivity; bullous diseases, including pemphigus, bullous pemphigoid, dermatitis herpetiformis, linear immunoglobulin A disease; pigmentation disorders, including hypopigmentation, vitiligo, albinism, post-inflammatory hypopigmentation, post-inflammatory hyperpigmentation, melasma, chloasma, drug-induced hyperpigmentation; disorders of cornification, including ichthyosis, keratosis pilaris, calluses, corns, actinic keratosis; pressure sores; disorders of sweating; inflammatory reactions including drug eruptions, toxic epidermal necrolysis; erythema multiforme, erythema nodosum, granuloma annulare.
In an embodiment, when the composition comprises l-arginine and optionally nicotinamide, the composition of the present invention is useful in treating a skin condition, selected from the group consisting of a skin infection, acne, rosacea, atopic dermatitis, eczema and psoriasis.
In additional embodiments, when the composition comprises l-arginine and optionally nicotinamide, the composition of the present invention is useful in treating the signs of ageing skin, including light-induced skin ageing, increased transepidermal water loss (TEWL), skin dryness, fine lines, wrinkles, hyperpigmentation and skin discoloration.
Yet, in additional embodiments, when the composition comprises l-arginine and optionally nicotinamide, the composition of the present invention is useful for application in concurrence with the dermal administration of a neurotoxin, such as botulism toxin A (“BTX”). It has been reported that intradermal injection of botulinum toxin A eliminates cutaneous vasodilation (Brett et al., TEMPERATURE 2017; 4/1:41-59); and by doing so, BTX reduces the supplies of oxygen and nutrients to the skin tissues. It has additionally been found that the topical administration of NO-donors potentiates the muscle relaxation effect of BTX (Lysy at al., Gut 2001; 48:221-224). Therefore, the composition of the present invention, which releases NO into the skin, can facilitate increased supplies of oxygen and nutrients to the skin tissues, augment the effect of BTX and prolong the duration of the effect. The composition can be used prior to BTX injection and then on a continuous basis following BTX injection. This composition can also be used to alleviate the inflammation that is induced following BTX injections.
The composition of the present invention, containing l-arginine and optionally nicotinamide, can further be useful in alleviating the atrophy of the skin due to BTX Injection.
As used in this written description, the singular forms “a,” “an” and “the” include express support for plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a gas” includes a plurality of such gases.
In this application, “comprises,” “comprising,” “containing” and “having” and the like can have the meaning ascribed to them in U.S. Patent law and can mean “includes,” “including,” and the like, and are generally interpreted to be open ended terms. The terms “consisting of” or “consists of” are closed terms, and include only the components, structures, steps, or the like specifically listed in conjunction with such terms, as well as that which is in accordance with U.S. Patent law. “Consisting essentially of” or “consists essentially of” have the meaning generally ascribed to them by U.S. Patent law. In particular, such terms are generally closed terms, with the exception of allowing inclusion of additional items, materials, components, steps, or elements, that do not materially affect the basic and novel characteristics or function of the item(s) used in connection therewith. For example, trace elements present in a composition, but not affecting the compositions nature or characteristics would be permissible if present under the “consisting essentially of” language, even though not expressly recited in a list of items following such terminology. When using an open-ended term, like “comprising” or “including,” in this written description it is understood that direct support should be afforded also to “consisting essentially of” language as well as “consisting of” language as if stated explicitly and vice versa.
The terms “first,” “second,” “third,” “fourth,” and the like in the description and in the claims, if any, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that any terms so used are interchangeable under appropriate circumstances such that the embodiments described herein are, for example, capable of operation in sequences other than those illustrated or otherwise described herein. Similarly, if a method is described herein as comprising a series of steps, the order of such steps as presented herein is not necessarily the only order in which such steps may be performed, and certain of the stated steps may possibly be omitted and/or certain other steps not described herein may possibly be added to the method.
The terms “left,” “right,” “front,” “back,” “top,” “bottom,” “over,” “under,” and the like in the description and in the claims, if any, are used for descriptive purposes and not necessarily for describing permanent relative positions. It is to be understood that the terms so used are interchangeable under appropriate circumstances such that the embodiments described herein are, for example, capable of operation in other orientations than those illustrated or otherwise described herein. The term “coupled,” as used herein, is defined as directly or indirectly connected in an electrical or nonelectrical manner. Objects described herein as being “adjacent to” each other may be in physical contact with each other, in close proximity to each other, or in the same general region or area as each other, as appropriate for the context in which the phrase is used. Occurrences of the phrase “in one embodiment,” or “in one aspect,” herein do not necessarily all refer to the same embodiment or aspect.
As used herein, comparative terms such as “increased,” “decreased,” “better,” “worse,” “higher,” “lower,” “enhanced,” “maximized,” “minimized,” and the like refer to a property of a device, component, or activity that is measurably different from other devices, components, or activities in a surrounding or adjacent area, in a single device or in multiple comparable devices, in a group or class, in multiple groups or classes, or as compared to the known state of the art. For example, a process that has an “increased” therapeutic effect or result can refer to improved results or efficacy attained by the process as compared to a similar or different process intended for treatment of the same condition or experience.
As used herein, the term “substantially” refers to the complete or nearly complete extent or degree of an action, characteristic, property, state, structure, item, or result. For example, an object that is “substantially” enclosed would mean that the object is either completely enclosed or nearly completely enclosed. The exact allowable degree of deviation from absolute completeness may in some cases depend on the specific context. However, generally speaking, the nearness of completion will be so as to have the same overall result as if absolute and total completion were obtained. The use of “substantially” is equally applicable when used in a negative connotation to refer to the complete or near complete lack of an action, characteristic, property, state, structure, item, or result. For example, a composition that is “substantially free of” particles would either completely lack particles, or so nearly completely lack particles that the effect would be the same as if it completely lacked particles. In other words, a composition that is “substantially free of” an ingredient or element may still actually contain such item as long as there is no measurable effect thereof.
As used herein, the term “about” is used to provide flexibility to a numerical range endpoint by providing that a given value may be “a little above” or “a little below” the endpoint. Unless otherwise stated, use of the term “about” in accordance with a specific number or numerical range should also be understood to provide support for such numerical terms or range without the term “about”. For example, for the sake of convenience and brevity, a numerical range of “about 50 angstroms to about 80 angstroms” should also be understood to provide support for the range of “50 angstroms to 80 angstroms.” Furthermore, it is to be understood that in this specification support for actual numerical values is provided even when the term “about” is used therewith. For example, the recitation of “about” 30 should be construed as not only providing support for values a little above and a little below 30, but also for the actual numerical value of 30 as well.
As used herein, a plurality of items, structural elements, compositional elements, and/or materials may be presented in a common list for convenience. However, these lists should be construed as though each member of the list is individually identified as a separate and unique member. Thus, no individual member of such list should be construed as a de facto equivalent of any other member of the same list solely based on their presentation in a common group without indications to the contrary.
Concentrations, amounts, and other numerical data may be expressed or presented herein in a range format. It is to be understood that such a range format is used merely for convenience and brevity and thus should be interpreted flexibly to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. As an illustration, a numerical range of “about 1 to about 5” should be interpreted to include not only the explicitly recited values of about 1 to about 5, but also include individual values and sub-ranges within the indicated range. Thus, included in this numerical range are individual values such as 2, 3, and 4 and sub-ranges such as from 1-3, from 2-4, and from 3-5, etc., as well as 1, 2, 3, 4, and 5, individually.
The term “subject” refers to a human or a non-human mammal.
This same principle applies to ranges reciting only one numerical value as a minimum or a maximum. Furthermore, such an interpretation should apply regardless of the breadth of the range or the characteristics being described.
Reference throughout this specification to “an example” means that a particular feature, structure, or characteristic described in connection with the example is included in at least one embodiment. Thus, appearances of the phrases “in an example” in various places throughout this specification are not necessarily all referring to the same embodiment.
PERSA GRATISSIMA
CHONDRUS CRISPUS f
MYRISTICA FRAGANS
CHAMOMILLA RECUTITA
Phase A
Phase B
Phase D
Phase E
Phase F
Phase G
Phase H
Package
Persa gratissima
fragans (nutmeg) extract
Chamomilla recutita
Objectives: To determine the impact of Compositions #1 and #3 on middle age women after 1 month. Methods: Two women over the age of 40 volunteered to receive a month supply of Composition #1 and Composition #2. They were asked to use both compositions daily for 28 days on two sides of the face and to provide input through post use questionnaire.
Results: The results are summarized in the following table.
Objectives: To determine the impact of Composition #1 on middle age women after 28 days of continuous use.
Methods: 20 women over the age of 40 in 3 countries (USA, Italy and Israel) volunteered to receive one-month supply of Composition #1 for use as their day cream. They were asked to use the cream daily for 28 days and to provide unguided input (free form)
Results: 13 of the 20 women provided feedback that was summarized in the table below.
8%
8%
2%
2%
2%
2%
2%
6%
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/IL2022/050057 | 1/13/2022 | WO |
| Number | Date | Country | |
|---|---|---|---|
| 63138702 | Jan 2021 | US | |
| 63137732 | Jan 2021 | US |
