Patent Application
20220362232
This disclosure relates to topical compositions for the treatment of inflammatory skin conditions, e.g., seborrheic dermatitis, eczema, and psoriasis. In particular, the disclosure provides topical compositions comprising a macrolide immunosuppressant (e.g., selected from pimecrolimus, sirolimus, or tacrolimus) and optionally, a triazole antifungal, specifically efinaconazole, and methods for making and using the same.
The macrolide immunosuppressants are a class of compounds consisting of a large macrocyclic lactam/lactone ring, including natural compounds from Streptomyces species and derivatives thereof, which bind to immunophilins and have a range of biological effects. While these compounds have potent biological activity, they are generally poorly soluble or insoluble in water, and so present formulation challenges.
Pimecrolimus is a 33-epi-chloro derivative of ascomycin, having the structure:
The exact mechanism of pimecrolimus is not known with certainty, but it is a calcineurin inhibitor, and appears to have immunosuppressive and anti-inflammatory effects due to its inhibition of various cytokine pathways. A 1% topical formulation of pimecrolimus (Elidel®) is approved for treatment of atopic dermatitis. Pimecrolimus is soluble in methanol and ethanol, but insoluble in water. Pimecrolimus has proved difficult to formulate, due to its relative insolubility.
Sirolimus, also known as rapamycin, is a macrolide compound obtained from Streptomyces hygroscopicus having the structure:
The exact mechanism of sirolimus is not known with certainty, but it has immunosuppressant, antifungal, and antineoplastic properties. It inhibits activation of T cells and B cells by reducing their sensitivity to interleukin-2 (IL-2) through mTOR inhibition. There are no topical formulations of sirolimus on the US market, but topical formulations of 0.1% or 1.0% sirolimus applied once daily have been investigated for treating facial angiofibromas, and oral tablet (1 or 2 mg) and solution (1 mg/mL) formulations have been sold under the trade name Rapamune®. Sirolimus is insoluble in water, but soluble in benzyl alcohol, chloroform, acetone, and acetonitrile. Due to its aqueous insolubility, sirolimus has proved difficult to formulate.
Tacrolimus, also known as fujimycin or FK-506, is a macrolide compound obtained from Streptomyces tsukubaensis having the structure:
The exact mechanism of tacrolimus is not known with certainty, but it is a calcineurin inhibitor, and it appears to have immunosuppressive and anti-inflammatory effects due to its inhibition of various cytokine pathways. A topical ointment formulation of tacrolimus (Protopic®) is available, having 0.03% or 0.1% of tacrolimus (w/w) in a base of mineral oil, paraffin, propylene carbonate, white petrolatum and white wax. Tacrolimus is insoluble in water, but soluble in benzyl alcohol, chloroform, acetone, and acetonitrile. Due to its aqueous insolubility, tacrolimus has proved difficult to formulate.
Efinaconazole is a triazole antifungal having the structure:
A 10% topical solution (Jublia®) is approved for treatment of treatment of onychomycosis of the toenails, e.g. caused by Trichophyton rubrum or Trichophyton mentagrophytes.
There is a need for improved treatments for inflammatory conditions of the skin. Formulations which combine different active ingredients are convenient for patients but may be constrained due to unpredictable chemical interactions between the active ingredients, unpredictable effects of one active ingredient on the delivery of another active ingredient, unpredictable efficacy, and potential for unpredictable side effects. Topical formulations of macrolide immunosuppressants, particularly in the form of topical creams, may be unstable due to precipitation of the macrolide immunosuppressant. There is a need for more stable formulations of macrolide immunosuppressants, and for combination formulations in which active ingredients are selected that are safe, effective and stable in combination with one another.
The disclosure provides a topical formulation comprising a macrolide immunosuppressant (e.g., selected from pimecrolimus, sirolimus, or tacrolimus) and efinaconazole in an emulsion base which is unexpectedly stable and useful in the treatment of inflammatory skin conditions. The presence of efinaconazole in the emulsion results in less or slower degradation of the macrolide, while the macrolide seems to have a beneficial effect on the stability of efinaconazole.
The disclosure provides, in one embodiment, a topical pharmaceutical composition comprising a macrolide immunosuppressant and efinaconazole, for example a topical cream formulation comprising 0.01 to 1.5 wt. % of macrolide immunosuppressant and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole. For example, the emulsion may be in the form of a cream, e.g., with a base comprising an oil phase (e.g., comprising alkyl diesters of aliphatic dicarboxylic acids), a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
In another embodiment, the disclosure provides a method of treating an inflammatory condition of the skin, for example seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising administering to the affected area, e.g., once or twice daily, a combination of a macrolide immunosuppressant and efinaconazole, for example in the form of a topical cream formulation comprising 0.01 to 1.5 wt. % of the macrolide immunosuppressant and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole, e.g., an emulsion as previously described.
In yet another embodiment, the disclosure provides a pharmaceutical formulation comprising a macrolide immunosuppressant and an alkyl diester of an aliphatic dicarboxylic acid, e.g., topical cream emulsion comprising 0.01 to 1.5 wt. % of the macrolide immunosuppressant, an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids, a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
For example, where the macrolide immunosuppressant is pimecrolimus, the disclosure provides a topical pharmaceutical composition comprising pimecrolimus and efinaconazole, for example a topical cream formulation comprising 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of pimecrolimus and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole. For example, the emulsion may be in the form of a cream, e.g., with a base comprising an oil phase (e.g., comprising alkyl diesters of aliphatic dicarboxylic acids), a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
In another embodiment, the disclosure provides a method of treating an inflammatory condition of the skin, for example seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising administering to the affected area, e.g., once or twice daily, a combination of pimecrolimus and efinaconazole, for example in the form of a topical cream formulation comprising 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of pimecrolimus and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole, e.g., an emulsion as previously described.
In yet another embodiment, the disclosure provides a pharmaceutical formulation comprising pimecrolimus and an alkyl diester of an aliphatic dicarboxylic acid, e.g., topical cream emulsion comprising 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of pimecrolimus, an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids, a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
Where the macrolide immunosuppressant is sirolimus, the disclosure provides a topical pharmaceutical composition comprising sirolimus and efinaconazole, for example a topical cream formulation comprising 0.1-1.5 wt. %, e.g., 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of sirolimus and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole. For example, the emulsion may be in the form of a cream , e.g., with a base comprising an oil phase (e.g., comprising alkyl diesters of aliphatic dicarboxylic acids), a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
In another embodiment, the disclosure provides a method of treating an inflammatory condition of the skin, for example seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising administering to the affected area, e.g., once or twice daily, a combination of sirolimus and efinaconazole, for example in the form of a topical cream formulation comprising 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of sirolimus and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole, e.g., an emulsion as previously described.
In yet another embodiment, the disclosure provides a pharmaceutical formulation comprising sirolimus and an alkyl diester of an aliphatic dicarboxylic acid, e.g., topical cream emulsion comprising 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of sirolimus, an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids, a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
Where the macrolide immunosuppressant is tacrolimus, the disclosure provides a topical pharmaceutical composition comprising tacrolimus and efinaconazole, for example a topical cream formulation comprising 0.01- 1 wt. %, e.g., about 0.03 wt % or 0.1 wt. % of tacrolimus and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole. For example, the emulsion may be in the form of a cream , e.g., with a base comprising an oil phase (e.g., comprising alkyl diesters of aliphatic dicarboxylic acids), a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
In another embodiment, the disclosure provides a method of treating an inflammatory condition of the skin, for example seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising administering to the affected area, e.g., once or twice daily, a combination of tacrolimus and efinaconazole, for example in the form of a topical cream formulation comprising 0.01-1 wt. %, e.g., about 0.03 wt % or 0.1 wt. % of tacrolimus and 0.5 to 3 wt. % (e.g., about 1 wt. % or about 2 wt. %) of efinaconazole, e.g., an emulsion as previously described.
In yet another embodiment, the disclosure provides a pharmaceutical formulation comprising tacrolimus and an alkyl diester of an aliphatic dicarboxylic acid, e.g., topical cream emulsion comprising 0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of tacrolimus, an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids, a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof), one or more gelling agents (e.g., comprising a carbomer), one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono- and di-glycerides), and combinations thereof), optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and optionally a chelator (e.g., edetate disodium (EDTA)).
Further areas of applicability of the present disclosure will become apparent from the detailed description provided hereinafter. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The following description of the preferred embodiment(s) is merely exemplary in nature and is in no way intended to limit the invention, its application, or uses.
The formulations herein are generally useful to treat inflammatory skin conditions. Inflammatory skin conditions include dermatitis (e.g., eczema (including atopic dermatitis), contact dermatitis, eczematous dermatitises, and seborrheic dermatitis), psoriasis (e.g., plaque psoriasis, guttate psoriasis, inverse psoriasis, pustular psoriasis, and erythrodermic psoriasis), lichen planus, lichen sclerosus, sclerosis, scleroderma, systemic sclerosis, hidradenitis suppurativa, pemphigus, bullous pemphigoid, epidermolysis bullosa, urticaria, angioedemas, vasculitides, erythemas (e.g., erythema multiforme, erythema nodosum), cutaneous eosinophilias, granuloma annulare, keratosis pilaris, panniculitis, pyoderma gangrenosum, Stevens-Johnson syndrome, toxic epidermal necrolysis, alopecia (e.g., alopecia areata, cicatricial alopecia), rosacea, and acne.
The formulations herein are particularly useful to treat seborrheic dermatitis, eczema or psoriasis.
In a particular embodiment, the formulations herein are useful to treat seborrheic dermatitis (also sometimes referred to as seborrheic eczema or seborrheic psoriasis, or cradle cap in infants), a common chronic inflammatory skin condition. Symptoms include scaly patches, red skin, and dandruff, usually on the scalp, but sometimes on the face or upper body. The causes of the condition are not fully understood but appear to involve interactions among the particular sebaceous gland secretions of the patient, the proliferation of Malessezia yeasts, and the patient's immune response.
In a first embodiment, the disclosure provides a topical pharmaceutical composition comprising a macrolide immunosuppressant and efinaconazole (Formulation A); for example,
In another embodiment, the disclosure provides a drug product, which is a container containing any of Formulations A, et seq., e.g., a pump container or a deformable tube containing any of Formulations A, et seq., e.g., a container comprising a pump and containing any of Formulations A, et seq., wherein the pump is calibrated to release a specific amount (e.g., 0.5-1 cubic centimeter, e.g., a pea-sized portion) of the formulation each time the pump is pressed.
In another embodiments, the disclosure provides a drug product in unit dose form (e.g., 0.5-1 cubic centimeter) comprising any of Formulations A, et seq.
In another embodiment, the disclosure provides a method (Method A) of treating inflammatory skin condition, e.g., seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising topically administering to the affected area an effective amount of macrolide immunosuppressant and efinaconazole, in combination, on at least a daily basis. For example, the disclosure provides:
In another embodiment, the disclosure provides the use of macrolide immunosuppressant and efinaconazole, in the manufacture of a medicament (e.g., a formulation according to any one of Formulations A, et seq.) for treatment of an inflammatory condition of the skin (e.g., in accordance with any of Methods A, et seq.).
In another embodiment, the disclosure provides a combination of macrolide immunosuppressant and efinaconazole for use in the treatment of inflammatory skin condition, e.g., seborrheic dermatitis, eczema, or psoriasis, e.g., in accordance with any of Methods A, et seq.
In another embodiment, the disclosure provides the use of efinaconazole to stabilize a macrolide immunosuppressant.
In another embodiment, the disclosure provides the use of a macrolide immunosuppressant to stabilize efinaconazole.
In one embodiment, the disclosure provides a method of stabilizing a macrolide immunosuppressant comprising admixing the macrolide immunosuppressant with efinaconazole, for example admixing to form any of Formulations A, et seq.
In one embodiment, the disclosure provides a method of stabilizing efinaconazole comprising admixing the efinaconazole with a macrolide immunosuppressant, for example admixing to form any of Formulations A, et seq.
As noted above, macrolide immunosuppressants have proved difficult to formulate, due to their relative insolubility. It is surprisingly found that macrolide immunosuppressants are particularly soluble and stable in alkyl diesters of aliphatic dicarboxylic acids, which can be used to provide pharmaceutical formulations having relatively high stability and reduced degradation and/or precipitation of macrolide immunosuppressant compared to prior art formulations of macrolide immunosuppressants.
In another embodiment, therefore, the disclosure provides a pharmaceutical formulation comprising a macrolide immunosuppressant and an alkyl diester of an aliphatic dicarboxylic acid, e.g.,
a topical cream formulation (Formulation B) comprising
0.01 to 1.5 wt. % of macrolide immunosuppressant,
an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids,
a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof),
one or more gelling agents (e.g., comprising a carbomer),
one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono-and di-glycerides), and combinations thereof),
optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and
optionally a chelator (e.g., edetate disodium (EDTA)).
For example, Formulation B includes the following formulations:
In another embodiment, the disclosure provides a method for treating an inflammatory skin condition, e.g., mild to moderate eczema (e.g., atopic dermatitis), seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising topical administration to the affected area of a topical cream formulation comprising a macrolide immunosuppressant and an alkyl diester of an aliphatic dicarboxylic acid, e.g., a formulation according to any of Formulation B, et seq.
In certain embodiments, the macrolide immunosuppressant is pimecrolimus. For example, the disclosure provides a topical pharmaceutical composition comprising pimecrolimus and efinaconazole (Formulation PE); for example,
In another embodiment, the disclosure provides a drug product, which is a container containing any of Formulations PE, et seq., e.g., a pump container or a deformable tube containing any of Formulations PE, et seq., e.g., a container comprising a pump and containing any of Formulations PE, et seq., wherein the pump is calibrated to release a specific amount (e.g., 0.5-1 cubic centimeter, e.g., a pea-sized portion) of the formulation each time the pump is pressed.
In another embodiments, the disclosure provides a drug product in unit dose form (e.g., 0.5-1 cubic centimeter) comprising any of Formulations PE, et seq.
In another embodiment, the disclosure provides a method (Method PE) of treating inflammatory skin condition, e.g., seborrheic dermatitis, eczema or psoriasis, in a patient in need thereof, comprising topically administering to the affected area an effective amount of pimecrolimus and efinaconazole, in combination, on at least a daily basis. For example, the disclosure provides:
In another embodiment, the disclosure provides the use of pimecrolimus and efinaconazole, in the manufacture of a medicament (e.g., a formulation according to any one of Formulations PE, et seq.) for treatment of an inflammatory condition of the skin (e.g., in accordance with any of Methods PE, et seq.).
In another embodiment, the disclosure provides a combination of pimecrolimus and efinaconazole for use in the treatment of inflammatory skin condition, e.g., seborrheic dermatitis, eczema, or psoriasis, e.g., in accordance with any of Methods PE, et seq.
In another embodiment, the disclosure provides the use of efinaconazole to stabilize pimecrolimus.
In another embodiment, the disclosure provides the use of pimecrolimus to stabilize efinaconazole.
In one embodiment, the disclosure provides a method of stabilizing pimecrolimus comprising admixing the pimecrolimus with efinaconazole, for example admixing to form any of Formulations PE, et seq.
In one embodiment, the disclosure provides a method of stabilizing efinaconazole comprising admixing the efinaconazole with pimecrolimus, for example admixing to form any of Formulations PE, et seq.
As noted above, pimecrolimus has proved difficult to formulate, due to its relative insolubility. It is surprisingly found that pimecrolimus is particularly soluble and stable in alkyl diesters of aliphatic dicarboxylic acids, which can be used to provide pharmaceutical formulations having relatively high stability and reduced degradation and/or precipitation of pimecrolimus compared to prior art formulations of pimecrolimus.
In another embodiment, therefore, the disclosure provides a pharmaceutical formulation comprising pimecrolimus and an alkyl diester of an aliphatic dicarboxylic acid, e.g.,
a topical cream formulation (Formulation P) comprising
0.5 to 1.5 wt. % (e.g., about 0.9 wt. %) of pimecrolimus,
an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids,
a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof),
one or more gelling agents (e.g., comprising a carbomer),
one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono-and di-glycerides), and combinations thereof),
optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and
optionally a chelator (e.g., edetate disodium (EDTA)).
For example, Formulation P includes the following formulations:
wherein all percentages are by weight of the formulation.
In another embodiment, the disclosure provides a method for treating an inflammatory skin condition, mild to moderate eczema (e.g., atopic dermatitis), seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising topical administration to the affected area of a topical cream formulation comprising pimecrolimus and an alkyl diesters of an aliphatic dicarboxylic acid, e.g., a formulation according to any of Formulation P, et seq.
In certain embodiments, the macrolide immunosuppressant is sirolimus. For example, the disclosure provides a topical pharmaceutical composition comprising sirolimus and efinaconazole (Formulation SE); for example,
wherein all percentages are by weight of the formulation.
In another embodiment, the disclosure provides a drug product, which is a container containing any of Formulations SE, et seq., e.g., a pump container or a deformable tube containing any of Formulations SE, et seq., e.g., a container comprising a pump and containing any of Formulations SE, et seq., wherein the pump is calibrated to release a specific amount (e.g., 0.5-1 cubic centimeter, e.g., a pea-sized portion) of the formulation each time the pump is pressed.
In another embodiments, the disclosure provides a drug product in unit dose form (e.g., 0.5-1 cubic centimeter) comprising any of Formulations SE, et seq.
In another embodiment, the disclosure provides a method (Method SE) of treating inflammatory skin condition, in a patient in need thereof, comprising topically administering to the affected area an effective amount of sirolimus and efinaconazole, in combination, on at least a daily basis. For example, the disclosure provides:
In another embodiment, the disclosure provides the use of sirolimus and efinaconazole, in the manufacture of a medicament (e.g., a formulation according to any one of Formulations SE, et seq.) for treatment of an inflammatory condition of the skin (e.g., in accordance with any of Methods SE, et seq.).
In another embodiment, the disclosure provides a combination of sirolimus and efinaconazole for use in the treatment of inflammatory skin condition, e.g., seborrheic dermatitis, eczema, or psoriasis, e.g., in accordance with any of Methods SE, et seq.
In another embodiment, the disclosure provides the use of efinaconazole to stabilize sirolimus.
In another embodiment, the disclosure provides the use of sirolimus to stabilize efinaconazole.
In one embodiment, the disclosure provides a method of stabilizing sirolimus comprising admixing the sirolimus with efinaconazole, for example admixing to form any of Formulations SE, et seq.
In one embodiment, the disclosure provides a method of stabilizing efinaconazole comprising admixing the efinaconazole with sirolimus, for example admixing to form any of Formulations SE, et seq.
As noted above, sirolimus has proved difficult to formulate, due to its relative insolubility. It is surprisingly found that sirolimus is particularly soluble and stable in alkyl diesters of aliphatic dicarboxylic acids, which can be used to provide pharmaceutical formulations having relatively high stability and reduced degradation and/or precipitation of sirolimus compared to prior art formulations of sirolimus.
In another embodiment, therefore, the disclosure provides a pharmaceutical formulation comprising sirolimus and an alkyl diester of an aliphatic dicarboxylic acid, e.g.,
a topical cream formulation (Formulation S) comprising
0.1 to 1.5 wt. % (e.g., about 0.9 wt. %) of sirolimus,
an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids,
a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof),
one or more gelling agents (e.g., comprising a carbomer),
one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono-and di-glycerides), and combinations thereof),
optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and
optionally a chelator (e.g., edetate disodium (EDTA)).
For example, Formulation S includes the following formulations:
In another embodiment, the disclosure provides a method for treating an inflammatory skin condition, e.g., mild to moderate eczema (e.g., atopic dermatitis), seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising topical administration to the affected area of a topical cream formulation comprising sirolimus and an alkyl diesters of an aliphatic dicarboxylic acid, e.g., a formulation according to any of Formulation S, et seq.
In certain embodiments, the macrolide immunosuppressant is tacrolimus. For example, the disclosure provides a topical pharmaceutical composition comprising tacrolimus and efinaconazole (Formulation TE); for example,
wherein all percentages are by weight of the formulation.
In another embodiment, the disclosure provides a drug product, which is a container containing any of Formulations TE, et seq., e.g., a pump container or a deformable tube containing any of Formulations TE, et seq., e.g., a container comprising a pump and containing any of Formulations TE, et seq., wherein the pump is calibrated to release a specific amount (e.g., 0.5-1 cubic centimeter, e.g., a pea-sized portion) of the formulation each time the pump is pressed.
In another embodiment, the disclosure provides a drug product in unit dose form (e.g., 0.5-1 cubic centimeter) comprising any of Formulations TE, et seq.
In another embodiment, the disclosure provides a method (Method 1E) of treating inflammatory skin condition, e.g., seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising topically administering to the affected area an effective amount of tacrolimus and efinaconazole, in combination, on at least a daily basis. For example, the disclosure provides:
In another embodiment, the disclosure provides the use of tacrolimus and efinaconazole, in the manufacture of a medicament (e.g., a formulation according to any one of Formulations TE, et seq.) for treatment of an inflammatory condition of the skin (e.g., in accordance with any of Methods TE, et seq.).
In another embodiment, the disclosure provides a combination of tacrolimus and efinaconazole for use in the treatment of inflammatory skin condition, e.g., seborrheic dermatitis, eczema, or psoriasis, e.g., in accordance with any of Methods TE, et seq.
In another embodiment, the disclosure provides the use of efinaconazole to stabilize tacrolimus.
In another embodiment, the disclosure provides the use of tacrolimus to stabilize efinaconazole.
In one embodiment, the disclosure provides a method of stabilizing tacrolimus comprising admixing the tacrolimus with efinaconazole, for example admixing to form any of Formulations TE, et seq.
In one embodiment, the disclosure provides a method of stabilizing efinaconazole comprising admixing the efinaconazole with tacrolimus, for example admixing to form any of Formulations TE, et seq.
As noted above, tacrolimus has proved difficult to formulate, due to its relative insolubility. It is surprisingly found that tacrolimus is particularly soluble and stable in alkyl diesters of aliphatic dicarboxylic acids, which can be used to provide pharmaceutical formulations having relatively high stability and reduced degradation and/or precipitation of tacrolimus compared to prior art formulations of tacrolimus.
In another embodiment, therefore, the disclosure provides a pharmaceutical formulation comprising tacrolimus and an alkyl diester of an aliphatic dicarboxylic acid, e.g.,
a topical cream formulation (Formulation T) comprising
0.01% to 1 wt. %, e.g., 0.01-0.1% wt. % of tacrolimus,
an oil phase comprising alkyl diesters of aliphatic dicarboxylic acids,
a water phase (e.g., comprising water and one or more alcohols, e.g., selected from (C2-4) mono- or poly-hydric alcohols, benzyl alcohol, and combinations thereof),
one or more gelling agents (e.g., comprising a carbomer),
one or more surfactants (e.g., selected from anionic surfactant (e.g., selected from sodium alkyl sulfates, e,g. sodium cetostearyl sulfate), nonpolar surfactants (e.g., selected from mono-and di-glycerides), and combinations thereof),
optionally an antioxidant (e.g., butylated hydroxytoluene (BHT)), and
optionally a chelator (e.g., edetate disodium (EDTA)).
For example, Formulation T includes the following formulations:
In another embodiment, the disclosure provides a method for treating an inflammatory skin condition, e.g., mild to moderate eczema (e.g., atopic dermatitis), seborrheic dermatitis, eczema, or psoriasis, in a patient in need thereof, comprising topical administration to the affected area of a topical cream formulation comprising tacrolimus and an alkyl diesters of an aliphatic dicarboxylic acid, e.g., a formulation according to any of Formulation T, et seq.
Unless stated otherwise, all percentages of composition components given in this specification are by weight based on a total composition or formulation weight of 100%.
The compositions and formulations as provided herein are described and claimed with reference to their ingredients, as is usual in the art. As would be evident to one skilled in the art, the ingredients may in some instances react with one another, so that the true composition of the final formulation may not correspond exactly to the ingredients listed. Thus, it should be understood that the invention extends to the product of the combination of the listed ingredients.
“About” with respect to an amount or a concentration means 80% to 120%, e.g., 90% to 110%, for example 95%-105% or +/−5% of the claimed value.
It will be understood that in certain formulations a particular ingredient may have more than one function. For example, relatively lipophilic nonpolar surfactants such as glycerol monostearate or glycerol distearate may form all or part of the oil phase of an emulsion formulation, as well as helping to keep the oil phase emulsified and stable.
As used throughout, ranges are used as shorthand for describing each and every value that is within the range. Any value within the range can be selected as the terminus of the range. In addition, all references cited herein are hereby incorporated by referenced in their entireties. In the event of a conflict in a definition in the present disclosure and that of a cited reference, the present disclosure controls.
Unless otherwise specified, all percentages and amounts expressed herein and elsewhere in the specification should be understood to refer to percentages by weight. The amounts given are based on the active weight of the material.
The invention is further illustrated in the following examples, which are meant to be exemplary and not limiting.
Three variations of a topical cream are manufactured at similar scale (500 to 1000 g), packaged in the same aluminum tube and placed on stability. Compositions of the 3 creams are provided in Table 1. The only difference in the three is the concentration of efinaconazole, which ranges from 0 to 2% w/w. A comparison of the total pimecrolimus-related substances in the 3 formulations at one month is shown in Table 2.
After one month at 40° C., no measurable degradants are noted in the creams containing both efinaconazole and pimecrolimus (Formulas B and C). On the other hand, Formula A (pimecrolimus alone, no efinaconazole) already exhibits 0.16% of total related substances.
A further experiment using larger batches of the three formulations of Table 1, studied for a longer period of time, further supports these results. Large size (35 Kg) batches of the 3 formulas in Table 1 are evaluated for stability under various storage conditions (three months at temperature up to 40° C., and relative humidity (RH) up to 75%). The lots are all manufactured at the same scale utilizing the same process and tested at about the same time using validated chemical analysis methods. Three-month data are now available and provided below in a chart and a table. These data confirm the conclusions from the earlier results—that the presence of efinaconazole stabilizes pimecrolimus in this base.
These results suggest mutually beneficial behavior of the two drug substances. In each case, chemical stability of pimecrolimus, as measured by total related substances, appears to be enhanced by the presence of efinaconazole.
The formula composition of the two products is provided in Table 3. Other than the difference in the drug substances (monad vs combination), the main difference is the composition of the oil phase solvents.
Test results of the total pimecrolimus-related substances are shown in Table 5. Six months of data at 40° C. are available and provided in the table. Difference in the total related substances between the two formulas is substantial at every time point. As before, the presence of the other drug substance—efinaconazole—appears to have significantly suppressed the degradation of pimecrolimus.
Moreover, comparing the stability of the pimecrolimus-only Formula A of Table 3 with the stability of the lotion in Table 5 suggests that Formula A shows superior stability to the pimecrolimus lotion, although it is not a side-by-side comparison.
Comparison of Pimecrolimus-only lotion and Pimecrolimus-Efinaconazole combination product at pH 5: Pimecrolimus in the above lotion base is discovered to be more susceptible to degradation at lower pH (around 5), compared to the combination product of Formula C, as seen in the level of total pimecrolimus-related substances shown in Table 6. The total related substances value is 2.2% of the original concentration after 3 months at 40° C. By comparison, the same value in the cream containing the combination of the drug substances is 0.74%—an improvement of 65% over the pimecrolimus-only product.
Data presented in the previous Examples support the hypotheses that the presence of efinaconazole results in less degradation of pimecrolimus in emulsions. It appears that the converse is also true, i.e. the presence of pimecrolimus aids in retarding the degradation of efinaconazole, as shown in Table 6. While the efinaconazole-only formulation is an alcohol-based solution and the combination formulation (Formula C from Example 1, supra) is an oil and water cream, the total efinaconazole-related substances are measurably lower in the combination product than in the efinaconazole-only product.
These test data suggest that when present together, pimecrolimus and efinaconazole act in a mutually beneficial manner, each helping in retarding the degradation of the other drug substance.
Three variations of a topical cream are manufactured at similar scale (500 to 1000 g), packaged in the same aluminum tube and placed on stability. Compositions of the 3 creams are provided in Table 7. The only difference in the three is the concentration of efinaconazole, which ranges from 0 to 2% w/w.
After one month at 40° C., no measurable degradants are noted in the creams containing both efinaconazole and sirolimus (Formulas B and C). On the other hand, Formula A (sirolimus alone, no efinaconazole) already exhibits formation of products of sirolimus degradation.
A further experiment using larger batches of the three formulations of Table 7 is conducted. Large size (35 Kg) batches of the 3 formulas in Table 7 are evaluated for stability under various storage conditions (three months at temperature up to 40° C., and relative humidity (RH) up to 75%). The lots are all manufactured at the same scale utilizing the same process and tested at about the same time using validated chemical analysis methods. Three-month data show that the presence of efinaconazole stabilizes sirolimus in this base.
The formula composition of the two products is provided in Table 8. Other than the difference in the drug substances (monad vs combination), the main difference is the composition of the oil phase solvents.
It is believed that sirolimus and efinaconazole act in a mutually beneficial manner, each helping in retarding the degradation of the other drug substance.
Three variations of a topical cream are manufactured at similar scale (500 to 1000 g), packaged in the same aluminum tube and placed on stability. Compositions of the 3 creams are provided in Table 9. The only difference in the three is the concentration of efinaconazole, which ranges from 0 to 2% w/w.
After one month at 40° C., no measurable degradants are noted in the creams containing both efinaconazole and tacrolimus (Formulas B and C). On the other hand, Formula A (tacrolimus alone, no efinaconazole) already exhibits formation of products of tacrolimus degradation.
A further experiment using larger batches of the three formulations of Table 1 is conducted. Large size (35 Kg) batches of the 3 formulas in Table 1 are evaluated for stability under various storage conditions (three months at temperature up to 40° C., and relative humidity (RH) up to 75%). The lots are all manufactured at the same scale utilizing the same process and tested at about the same time using validated chemical analysis methods. Three-month data show that the presence of efinaconazole stabilizes tacrolimus in this base.
The formula composition of the two products is provided in Table 10. Other than the difference in the drug substances (monad vs combination), the main difference is the composition of the oil phase solvents.
It is believed that tacrolimus and efinaconazole act in a mutually beneficial manner, each helping in retarding the degradation of the other drug substance.
This application claims the benefit of U.S. Provisional Application No. 62/964,495, filed Jan. 22, 2020; U.S. Provisional Application No. 62/964,507, filed Jan. 22, 2020; and U.S. Provisional Application No. 62/964,516, filed Jan. 22, 2020; and the entire contents of each of these applications is incorporated herein by reference.
| Number | Date | Country | |
|---|---|---|---|
| 62964516 | Jan 2020 | US | |
| 62964507 | Jan 2020 | US | |
| 62964495 | Jan 2020 | US |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/EP2021/051478 | Jan 2021 | US |
| Child | 17814474 | US |
