Topical Preparation For Application To The Skin Containing Natural Oil Of The Evening Primrose (Oenothera Biennis) (=Oleum Oenotherae) And Osmolytes Originating From Extremophilic Microorganisms

Abstract

The invention relates to formulations comprising natural oils and fats that contain an osmolyte originating from extremophilic microorganisms and unsaturated fatty acids, with the osmolyte being present in an amount ranging between 0.01 and 50 wt. % based on the total weight of the formulation. The invention also relates to the use of osmolytes originating from extremophilic microorganisms for the stabilization of unsaturated fatty acids. Especially preferred is a combination of osmolyte and evening primrose oil (Oleum Oenotherae).

Description

EXAMPLE 1

O/W Lotion

According to the invention an osmolytes X and evening primrose oil (Oleum Oenotherae) containing lotion (O/W) is made from the following components:

                             Percentage
Components                   by Weight (% w/v)
Evening primrose oil (Oleum  8.00
Oenotherae)
Isopropyl palmitate          3.00
Petrofatum                   4.00
Cetylstearyl alcohol         2.00
Sodium cetylstearyl sulfate  0.50
Sodium carbomer              0.40
Osmolyte ectoine             0.50
Octylmethooxycinnamate       5.00
Butylmethoxydibenzoylmethane 1.00
Water                        Ad 100.00

EXAMPLE 2

O/W Cream

According to the invention an osmolyte X containing cream (O/W) is made from the following components:

                                 Percentage
Components                       by Weight (% w/v)
Evening primrose oil (Oleum Oenotherae) 10.00
Glycerylmonostearate             4.00
Osmolyte X                       0.50
Titanium dioxide                 1.00
Sodium lactate                   3.00
Water                            Ad 100.00

EXAMPLE 3

O/W Cream with Cyclic Diphosphoglycerate (cDPG)

According to the invention an osmolyte cyclic diphosphoglycerate (cDPG)-containing cream (O/W) is made from the following components:

                                 Percentage
Components                       by Weight (% w/v)
Evening primrose oil (Oleum Oenotherae) 10.00
Glycerylmonostearate             4.00
Osmolyte cDPG                    0.50
Titanium dioxide                 1.00
Sodium lactate                   3.00
Water                            Ad 100.00

EXAMPLE 4

Stabilization of a Solution Consisting of γ-linolenic Acid Obtained from Evening Primrose Plants Using the Osmolyte Diglycerol Phosphate (DGP)

The stability of evening primrose oil at higher temperatures (room temperature, 25° C.) is largely dependent on the γ-linolenic acid content and the purity of the evening primrose oil. After 0.1% (wt. percent) of diglycerol phosphate had been added to a preparation of evening primrose oil an improved evening primrose oil storage stability of five days was noticed compared to a check preparation that did not contain diglycerol phosphates. The stability of the preparation was assessed based on the first occurrence of a rancid smell of the evening primrose oil.

EXAMPLE 5

Improved Temperature Stability of Evening Primrose Oil When an Osmolyte Blend of Mannosylglycerate (Firoin) and Mannosylglyceramide (Firoin-A) Was Added

Increasing the temperature to 70° C. within a period of one hour causes evening primrose oil to become quickly destabilized/denaturized with hydroperoxides forming. These highly reactive molecules may then form polymers (resinification, drying up) and low-molecular fragments causing a rancid odor. A 50:50 blend of the osmolytes mannosylglycerate and mannosylglyceramide of a 5 wt. % concentration enhances the resistance of evening primrose oil to oxidative changes at temperatures of 70° C. by a time span of 24 hours. In the presence of a 50-% mannosylglycerate-mannosylglyceramide mixture a rancid odor can only be noticed after a 24-hour incubation period at 70° C. The use of a mannosylglycerate-mannosylglyceramide mixture can thus significantly improve, for example, the processing and storage properties of evening primrose oil and of products containing evening primrose oil.

EXAMPLE 6

Gel Containing Evening Primrose Oil and the Osmolyte Diinositol Phosphate

According to the invention a gel (O/W) containing diinositol phosphate and evening primrose oil is made from the following components:

                                 Percentage
Components                       by Weight (% w/v)
Carbopol                         2.00
Triethanolamine                  3.00
Di-inositol phosphate            0.50
Evening primrose oil             10.00
Tocopherylacetate                0.20
Polyoxyethylene sorbitan fatty acid ester 0.50
(Tween 20)
Glycerine                        2.00
Sodium PCA                       0.50
Hydrolized collagen              2.00
Preservative agents, coloring matter, q.s.
perfume
Water                            Ad 100.00

As preservative agent 0.05% propylhydroxybenzoate or 0.15% methyl-4-hydroxybenzoate may be used.

EXAMPLE 7

Sunscreen Emulsion Containing Evening Primrose Oil and the Osmolyte Hydroxyectoine

According to the invention a sunscreen emulsion containing evening primrose oil and hydroxectoine is made from the following components:

                                 Percentage by
Components                       Weight (% w/v)
Cyclomethicone                   2.00
Evening primrose oil             10%
Cetyldimethicone copolyol        0.20
PEG 22 dodecyl copolymer         3.00
Paraffin oil (DAB 9)             2.00
Caprylic acid/Caprinic acid triglyceride 5.80
Octylmethoxycinnamate            5.80
Butyl methoxy dibenzoylmethane   4.00
Tocopherylacetate                0.50
ZnSO4                            0.70
Na3IIEDTA                        0.30
Hydroxyectoine                   2.50
Preservative agents, coloring matter, perfume q.s.
Water                            ad 100.00

Claims

1. Formulation comprising natural oils and fats containing an osmolyte originating from extremophilic microorganisms and unsaturated fatty acids, characterized in that said formulation contains 0.01 to 50 wt. % of at least one osmolyte based on the total weight of the formulation.

2. Formulation according to claim 1, characterized in that said formulation contains 0.05 to 10 wt. %, particularly 0.1 to 5 wt. % of an osmolyte or of osmolytes.

3. Formulation according to claim 1 or 2, characterized in that it contains 0.01 to 50 wt. % of natural oils and fats.

4. Formulation according to claim 3, characterized in that said formulation contains 0.05 to 10 wt. %, particularly 0.1 to 50 wt. % of natural oils an fats.

5. Formulation according to at least one of the above claims, characterized in that it contains evening primrose oil (Oleum Oenotherae).

6. Formulation according to at least one of the above claims, characterized in that ectoine, hydroxyectoine, cDPG, DGP, firoin, firoin A and/or diinositol phosphate are used as osmolyte.

7. Formulation according to any one of the claims 1 to 6 for cosmetic application to the skin.

8. Cosmetic formulation according to claim 7, characterized in that it is provided in the form of a solution, a suspension, an emulsion, a paste, an ointment, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing agent, an oil, a lipstick, a lip-care stick, a mascara, an eyeliner, of eye shadowing, rouge, a powder, emulsion and wax make-up, a sunscreen, pre-sun and after-sun preparation, a hair tonic, a plaster, a bandage or spray.

9. Use of an osmolyte originating from extremophilic microorganisms for the stabilization and/or preservation of unsaturated fatty acids.

10. Use according to claim 9, characterized in that the applied osmolyte is present in an amount of between 0.01 and 50 wt. % in relation to the entire formulation.

11. Use according to claim 9 or 10, characterized in that it contains 0.05 to 10 wt. %, particularly 0.1 to 5 wt. % of an osmolyte or of osmolytes.

12. Use according to at least one of the claims 9 to 11, characterized in that ectoine, hydroxyectoine, cDPG, DGP, firoin, firoin A and/or diinositol phosphate are used as osmolyte.

13. Use according to at least one of the claims 9 to 12 for the stabilization of evening primrose oil (Oleum Oenotherae).

14. Use of an osmolyte originating from extremophilic microorganisms for the production of formulations for topical applications in the cosmetic and dermatological field.

15. Use according to claim 14 for the production of cosmetic formulations for the tending and care of very dry, irritated, scaly and problematic skin, for the tending and care of inflamed skin in case of atopic dermatitis, psoriasis and other inflammable skin diseases, for the protection and stabilization of human skin cells against physical, chemical and biological influences, in particular UV and IR radiation and denaturizing substances, for the protection of the skin's microflora, for the stabilization of the natural skin barrier and as free-radical scavenger and/or antioxidants.

16. Use according to claim 14 for the production of medicinal products and/or medicaments for the tending, care, prophylaxis or treatment of atopic dermatitis, psoriasis, other inflammable skin diseases as well as eczema.

17. Use according to any one of claims 14 to 16, characterized in that the osmolyte is ectoine or hydroxyectoine.

18. Use according to at least one of the claims 14 to 17 in the form of a solution, a suspension, an emulsion, a paste, an ointment, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing agent, an oil, a lipstick, a lip-care stick, a mascara, an eyeliner, of eye shadowing, rouge, a powder, emulsion or wax make-up, a sunscreen, pre-sun and after-sun preparation, a hair tonic, a plaster, a bandage or spray.

19. Use according to claim 18, characterized in that the formulation further contains at least one UV filter, enzymes, vitamins, vitamin derivatives and/or proteins.