Claims
- 1. A method for enhancing tissue culture response in a plant cell comprising introducing into the plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide.
- 2. The method of claim 1 wherein the plant cell is transformed with at least one LEC1 polynucleotide.
- 3. The method of claim 1 wherein at least one polynucleotide is operably linked to a promoter driving expression in the plant cell and growing the plant cell.
- 4. The method of claim 1 wherein the plant cell is a recalcitrant cell.
- 5. The method of claim 1 wherein the plant cell is an inbred plant cell.
- 6. A method for inducing somatic embryogenesis in a plant cell comprising introducing into a plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide, wherein the plant cell is transformed and grown to produce a transformed embryo and wherein the plant cell is not an Arabidopsis cell.
- 7. The method of claim 6 wherein the plant cell is transformed with at least one LEC1 polynucleotide.
- 8. The method of claim 6 wherein at least one polynucleotide is operably linked to a promoter driving expression in the plant cell.
- 9. The method of claim 6 further comprising growing the transformed embryo under plant growing conditions to produce a regenerated plant.
- 10. A transgenic plant produced by the method of claim 9.
- 11. The method of claim 6 wherein the plant cell is from corn, soybean, sorghum, wheat, rice, alfalfa, sunflower, canola or cotton.
- 12. A method for positive selection of a transformed cell comprising introducing into a plant cell at least one LEC1 polynucleotide or at least one LEC1 polypeptide and growing the transformed plant cell, wherein the plant cell is transformed and grown under conditions sufficient to induce embryogenesis to provide a positive selection means.
- 13. The method of claim 12 further comprising altering media components to favor the growth of transformed plant cells.
- 14. The method of claim 13 wherein the media components are altered to reduce somatic embryogenesis in non-transformed cells.
- 15. The method of claim 12 wherein the plant cell is transformed with at least one LEC1 polynucleotide.
- 16. The method of claim 15 wherein at least one LEC1 polynucleotide is operably linked to a promoter driving expression in a plant.
- 17. The method of claim 16 wherein the polynucleotide is excised.
- 18. The method of claim 15 wherein the polynucleotide is flanked by FRT sequences to allow FLP mediated excision of the polynucleotide.
- 19. A method for inducing apomixis in a plant cell comprising introducing into a responsive plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide and growing the plant cell, wherein the introducing and growing is done under conditions sufficient to produce a transformed somatic embryo.
- 20. The method of claim 19 wherein the plant cell is transformed with at least one LEC1 polynucleotide.
- 21. The method of claim 19 wherein the at least one polynucleotide is expressed in integument or nucellus tissue.
- 22. The method of claim 19 wherein the at least one polynucleotide is operably linked to a promoter driving expression in a plant cell
- 23. The method of claim 22 further comprising suppressing in the plant cell expression of an FIE polycomb polynucleotide.
- 24. The method of claim 22 wherein the promoter is an inducible promoter.
- 25. The method of claim 19 further comprising growing the transformed somatic embryo under plant growing conditions to produce a regenerated plant.
- 26. A plant produced by the method of claim 25.
- 27. The plant of claim 26, wherein the plant is male sterile.
- 28. A method for increasing transformation efficiency comprising introducing at least one LEC1 polypeptide or at least one LEC1 polynucleotide into a plant cell.
- 29. The method of claim 28 wherein the plant cell is transformed with at least one LEC1 polynucleotide.
- 30. The method of claim 29 wherein plant cell is in contact with medium that retards growth of somatic embryo growth in non-transformed plants.
- 31. The method of claim 29 wherein transformation is conducted with reduced levels of auxin or no auxin.
- 32. The method of claim 29 wherein the at least one polynucleotide is operably linked to a promoter driving expression in a plant cell
- 33. The method of claim 28 wherein the plant cell is a recalcitrant cell.
- 34. The method of claim 28 wherein the plant cell is an inbred cell.
- 35. A method for increasing recovery of regenerated plants comprising introducing into a plant cell at least one LEC1 polypeptide or at least one LEC1 polynucleotide and growing the plant cell.
- 36. The method of claim 35 wherein the plant cell is transformed with at least one LEC1 polynucleotide.
- 37. The method of claim 35 wherein the at least one polynucleotide is operably linked to a promoter driving expression in a plant cell
- 38. The method of claim 35 wherein the plant cell is a recalcitrant cell.
- 39. The method of claim 35 wherein the plant cell is an inbred plant cell.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This is a divisional application that claims priority to U.S. application Ser. No. 09/435,054 filed Nov. 8, 1999; U.S. Provisional Applications Serial No. 60/107,643 filed Nov. 9, 1998 and U.S. Provisional Application No. 60/107,810 filed Nov. 10, 1998.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60107643 |
Nov 1998 |
US |
|
60107810 |
Nov 1998 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09435054 |
Nov 1999 |
US |
Child |
10744149 |
Dec 2003 |
US |