Claims
- 1. A transgenic plant comprising
- (a) at least one gene, operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
- 2. A transgenic plant comprising
- (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and
- (b) a AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
- 3. A transgenic fungus-resistant plant according to claim 1 or 2 wherein the plant is a tobacco, potato, strawberry, corn, rape or tomato plant.
- 4. A purified and isolated DNA molecule comprising
- (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8.
- 5. A purified and isolated DNA molecule comprising
- (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and
- (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1.
- 6. A process for the generation of a fungus-resistant plant according to claim 1 or 2, wherein a plant is transformed with at least one gene with fungus-inhibiting action, and the fungus-resistant plant is obtained by a method selected from the group consisting of
- (a) crossing the plant with another plant which contains at least one other gene, said gene having fungus-inhibiting action, and subsequently selecting the fungus-resistant plant, and
- (b) transforming the plant with at least one other gene, said gene having fungus-inhibiting action.
- 7. A process according to claim 6 which employs a DNA transfer vector comprising a gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9,
- a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11,
- a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4,
- an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8.
- 8. A process for the generation of a transgenic fungus-resistant plant comprising employing a DNA-vector comprising
- (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and
- (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, for transformation of a plant.
- 9. A process for the generation of transgenic fungus-resistant plant, comprising employing a DNA vector comprising
- (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and
- (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, for transformation of a plant.
Priority Claims (1)
Number |
Date |
Country |
Kind |
42 34 131.0 |
Oct 1992 |
DEX |
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Parent Case Info
This application is a continuation of application Ser. No. 08/134,416, filed on Oct. 8, 1993, now abandoned.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
4940840 |
Suslow et al. |
Jul 1990 |
|
4970168 |
Tumer |
Nov 1990 |
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Foreign Referenced Citations (2)
Number |
Date |
Country |
8904371 |
May 1989 |
EPX |
0440304 |
Aug 1991 |
EPX |
Continuations (1)
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Number |
Date |
Country |
Parent |
134416 |
Oct 1993 |
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