Claims
- 1. A transgenic plant wherein at least a part of said plant comprises a recombinant nucleic acid comprising a promoter active in said part operably linked to a nucleic acid encoding a thioredoxin polypeptide wherein said promoter is a seed or grain maturation-specific promoter and said thioredoxin polypeptide comprises the amino acid sequence WCGPC or WCPPC (SEQ ID NO: 1).
- 2. The transgenic plant of claim 1 wherein said part is a seed.
- 3. The transgenic plant of claim 1 wherein said part is a grain.
- 4. The transgenic plant of claim 1 wherein said promoter is selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, rice globulins, barley hordeins, wheat gliadins, wheat glutenins, maize zeins, maize glutelins, oat glutelins, sorghum kafirins, millet pennisetins, rye secalins, and maize embryo-specific globulin promoters.
- 5. The transgenic plant of claim 1 wherein said barley hordein promoter is selected from the group consisting of B1-hordein and D-hordein promoters.
- 6. The transgenic plant of claim 1 wherein said plant is a monocot.
- 7. The transgenic plant of claim 6 wherein said monocot plant is selected from the group consisting of rice, barley, maize, wheat, oat, rye, sorghum, millet, triticale, turfgrass and forage grass.
- 8. The transgenic plant of claim 1 wherein said thioredoxin is thioredoxin h.
- 9. The transgenic plant of claim 1 wherein said recombinant nucleic acid further comprises a nucleic acid encoding a signal peptide operably linked to said promoter and said nucleic acid molecule encoding a thioredoxin protein.
- 10. The transgenic plant of claim 9 wherein said signal peptide targets expression of the thioredoxin polypeptide to an intracellular body.
- 11. The transgenic plant of claim 9 wherein said signal peptide is selected from the group consisting of B1-hordein and D-hordein signal peptides.
- 12. The transgenic plant of claim 9 wherein said promoter is selected from the group consisting of rice glutelins, rice oryzins, rice prolamines, rice globulins, barley hordeins, wheat gliadins, wheat glutenins, maize zeins, maize glutelins, oat glutelins, sorghum kafirins, millet pennisetins, rye secalins, and maize embryo-specific globulin promoters.
- 13. A transgenic plant produced from a non-transgenic parent plant or plant cell comprising a recombinant thioredoxin protein wherein said recombinant thioredoxin protein includes the amino acid sequence WCGPC or WCPPC (SEQ ID NO: 1) and said recombinant thioredoxin protein increases the in vivo reduction of thiol groups on one or more proteins in said transgenic plant by at least 5% compared to the in vivo reduction of said one or more proteins in said non-transgenic parent plant or plant cell.
- 14. The plant of claim 13 wherein said proteins are selected from the group consisting of members of the alpha-amylase inhibitor, the alpha-amylase/trypsin inhibitor and the sulfur-rich gliadin families of proteins.
- 15. The transgenic plant of claim 13 wherein said plant is a monocot.
- 16. The transgenic plant of claim 13 wherein said plant is a dicot.
- 17. The transgenic plant of claim 15 wherein said moncot is selected from the group consisting of maize, rice, wheat, sorghum and barley.
RELATED APPLICATIONS
[0001] This application claims priority to U.S. Provisional Patent Application No. 60/307,006 filed Jul. 19, 2001 and is a continuation-in-part application of U.S. application Ser. No. 09/538,864 filed Mar. 29, 2000 which claims priority to U.S. Provisional Patent application No. 60/126,736 filed Mar. 29, 1999 all of which are hereby incorporated by reference in their entirety.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60307006 |
Jul 2001 |
US |
|
60126736 |
Mar 1999 |
US |
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09538864 |
Mar 2000 |
US |
Child |
10194885 |
Jul 2002 |
US |