Transgenic plants with enhanced agronomic traits

Abstract
This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
Description
INCORPORATION OF SEQUENCE LISTING

Two copies of the sequence listing (Copy 1 and Copy 2) and a computer readable form (CRF) of the sequence listing, all on CD-Rs, each containing the text file named 38-21(54973)A_seqListing.txt, which is 105,877,504 bytes (measured in MS-WINDOWS), were created on Jul. 10, 2008 and are herein incorporated by reference.


INCORPORATION OF COMPUTER PROGRAM LISTING

Two copies of the Computer Program Listing (Copy 1 and Copy 2) and a computer readable form (CRF) containing folders hmmer-2.3.2 and 226pfamDir, all on CD-Rs are incorporated herein by reference in their entirety. Folder Hmmer-2.3.2 contains the source code and other associated file for implementing the HMMer software for Pfam analysis. Folder 226pfamDir contains 226 Pfam Hidden Markov Models. Both folders were created on CD-R on Jul. 9, 2007, having a total size of 19,894,272 bytes (measured in MS-WINDOWS).


FIELD OF THE INVENTION

Disclosed herein are recombinant DNA useful for providing enhanced traits to transgenic plants, seeds, pollen, plant cells and plant nucleui of such transgenic plants, methods of making and using such recombinant DNA, plants, seeds, pollen, plant cells and plant nuclei. Also disclosed are methods of producing hybrid seed comprising such recombinant DNA.


SUMMARY OF THE INVENTION

This invention employs recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic traits to the transgenic plants. Recombinant DNA in this invention is provided in a construct comprising a promoter that is functional in plant cells and that is operably linked to a DNA segment that encodes a protein. In some embodiments of the invention, such protein defined by protein domains e.g. a “Pfam domain module” (as defined herein below) from the group of Pfam domain modules identified in Table 11. In other embodiments of the invention, e.g. where a Pfam domain module is not available, such protein is defined a consensus amino acid sequence of an encoded protein that is targeted for production e.g. a protein having amino acid sequence with at least 90% identity to a consensus amino acid sequence in the group of SEQ ID NO: 30526 through SEQ ID NO: 30550. In more specific embodiments of the invention the protein expressed in plant cells is a protein selected from the group of proteins identified in Table 2 and their homologs identified in Table 8.


Other aspects of the invention are specifically directed to plant cell nuclei and transgenic cells comprising the recombinant DNA construct of the invention, transgenic plants comprising a plurality of such plant cells, progeny transgenic seed, embryo and transgenic pollen from such plants. Such transgenic plants are selected from a population of transgenic plants regenerated from plant cells transformed with recombinant DNA construct and expressed the protein by screening transgenic plants in the population for an enhanced trait as compared to control plants that do not have the recombinant DNA construct, where the enhanced trait is selected from group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.


In yet another aspect of the invention the plant cell nuclei, plant cells, transgenic plants, seeds, and pollen further comprise recombinant DNA expressing a protein that provides tolerance from exposure to an herbicide applied at levels that are lethal to a wild type plant cell. Such tolerance is especially useful not only as an advantageous trait in such plants but is also useful in a selection step in the methods of the invention. In aspects of the invention such herbicide is a glyphosate, dicamba, or glufosinate compound.


Yet other aspects of the invention provide transgenic plants which are homozygous for the recombinant DNA and transgenic seed of the invention from corn, soybean, cotton, canola, alfalfa, wheat or rice plants.


This invention also provides methods for manufacturing non-natural, transgenic seed that can be used to produce a crop of transgenic plants with an enhanced trait resulting from expression of stably-integrated, recombinant DNA construct provided by herein. More specifically the method comprises (a) screening a population of plants for an enhanced trait and a recombinant DNA construct of the invention, where individual plants in the population can exhibit the trait at a level less than, essentially the same as or greater than the level that the trait is exhibited in control plants which do not express the recombinant DNA, (b) selecting from the population one or more plants that exhibit the trait at a level greater than the level that said trait is exhibited in control plants, and (c) collecting seed from a selected plant. The method further comprises (d) verifying that the recombinant DNA construct is stably integrated in said selected plants, and (e) analyzing tissue of a selected plant to determine the production of a protein having the function of a protein selected from SEQ ID NO: 299 through SEQ ID NO: 30468. In one aspect of the invention the plants in the population further comprise DNA expressing a protein that provides tolerance to exposure to a herbicide applied at levels that are lethal to wild type plant cells and the selecting is affected by treating the population with the herbicide, e.g. a glyphosate, dicamba, or glufosinate compound. In another aspect of the invention the plants are selected by identifying plants with the enhanced trait. The methods are especially useful for manufacturing corn, soybean, cotton, canola, alfalfa, wheat or rice seed.


Another aspect of the invention provides a method of producing hybrid corn seed comprising acquiring hybrid corn seed from a herbicide tolerant corn plant which also has stably-integrated, recombinant DNA construct comprising a promoter that is (a) functional in plant cells and (b) is operably linked to DNA that encodes a protein provided by the invention. The methods further comprise producing corn plants from the hybrid corn seed, wherein a fraction of the plants produced from said hybrid corn seed is homozygous for said recombinant DNA, a fraction of the plants produced from said hybrid corn seed is hemizygous for the recombinant DNA construct, and a fraction of the plants produced from said hybrid corn seed has none of the recombinant DNA construct; selecting corn plants which are homozygous and hemizygous for the recombinant DNA construct by treating with an herbicide; collecting seed from herbicide-treated-surviving corn plants and planting the seed to produce further progeny corn plants; repeating the selecting and collecting steps at least once to produce an inbred corn line; and crossing the inbred corn line with a second corn line to produce hybrid seed.





BRIEF DESCRIPTION OF THE DRAWINGS


FIG. 1 is a consensus amino acid sequence of SEQ ID NO: 301 and its homologs.



FIGS. 2-4 are plasmid maps.





DETAILED DESCRIPTION OF THE INVENTION

In the attached sequence listing:


SEQ ID NO:1-298 are nucleotide sequences of the coding strand of DNA for “genes” used in the recombinant DNA imparting an enhanced trait in plant cells, i.e. each represents a coding sequence for a protein;


SEQ ID NO: 299-596 are amino acid sequences of the cognate protein of the “genes” with nucleotide coding sequences 1-298;


SEQ ID NO: 597-30468 are amino acid sequences of homologous proteins;


SEQ ID NO: 30469-30520 are nucleotide sequences of the elements in base plasmid vectors;


SEQ ID NO: 30521 is a nucleotide sequence of a base plasmid vector useful for corn transformation;


SEQ ID NO: 30522 is a nucleotide sequence of a base plasmid vector useful for soybean and canola transformation;


SEQ ID NO: 30523 is a nucleotide sequence of a base plasmid vector useful for cotton transformation;


SEQ ID NO: 30524 is a nucleotide sequence of a Sphas1 promoter from soybean;


SEQ ID NO: 30525 is a nucleotide sequence of a Sphas1 leader from soybean.


SEQ ID NO: 30526-30550 are consensus sequences.


Table 1 lists the protein SEQ ID NOs and their corresponding consensus SEQ ID NOs.











TABLE 1





Gene ID
PEP SEQ ID NO
Consensus SEQ ID NO







PHE0006007_18714
301
30526


PHE0006460_15962
453
30527


PHE0006657_16192
334
30528


PHE0006712_16273
415
30529


PHE0006859_16873
376
30530


PHE0006907_16797
594
30531


PHE0006936_16828
348
30532


PHE0006969_16871
428
30533


PHE0007578_17852
446
30534


PHE0007650_18175
358
30535


PHE0008172_18471
397
30536


PHE0008308_18841
392
30537


PHE0008340_19155
410
30538


PHE0008422_18842
412
30539


PHE0008698_24421
364
30540


PHE0010197_21429
569
30541


PHE0010201_21433
576
30542


PHE0010201_21768
574
30543


PHE0010223_21491
545
30544


PHE0010397_21762
538
30545


PHE0010398_21763
539
30546


PHE0010615_22400
510
30547


PHE0010838_22702
575
30548


PHE0011447_23660
586
30549


PHE0012170_24424
572
30550









As used herein a “plant cell” means a plant cell that is transformed with stably-integrated, non-natural, recombinant DNA construct, e.g. by Agrobacterium-mediated transformation or by baombardment using microparticles coated with recombinant DNA construct or other means. A plant cell of this invention can be an originally-transformed plant cell that exists as a microorganism or as a progeny plant cell that is regenerated into differentiated tissue, e.g. into a transgenic plant with stably-integrated, non-natural recombinant DNA, or seed or pollen derived from a progeny transgenic plant.


As used herein a “transgenic plant” means a plant whose genome has been altered by the stable integration of recombinant DNA construct. A transgenic plant includes a plant regenerated from an originally-transformed plant cell and progeny transgenic plants from later generations or crosses of a transformed plant.


As used herein “recombinant DNA” means DNA which has been a genetically engineered and constructed outside of a cell including DNA containing naturally occurring DNA or cDNA or synthetic DNA.


As used herein “consensus sequence” means an artificial sequence of amino acids in a conserved region of an alignment of amino acid sequences of homologous proteins, e.g. as determined by a CLUSTALW alignment of amino acid sequence of homolog proteins.


As used herein a “homolog” means a protein in a group of proteins that perform the same biological function, e.g. proteins that belong to the same Pfam protein family and that provide a common enhanced trait in transgenic plants of this invention. Homologs are expressed by homologous genes. Homologous genes include naturally occurring alleles and artificially-created variants. Degeneracy of the genetic code provides the possibility to substitute at least one base of the protein encoding sequence of a gene with a different base without causing the amino acid sequence of the polypeptide produced from the gene to be changed. Hence, a polynucleotide useful in the present invention may have any base sequence that has been changed from SEQ ID NO:1 through SEQ ID NO: 298 substitution in accordance with degeneracy of the genetic code. Homologs are proteins that, when optimally aligned, have at least 60% identity, more preferably about 70% or higher, more preferably at least 80% and even more preferably at least 90% identity over the full length of a protein identified as being associated with imparting an enhanced trait when expressed in plant cells. Homologs include proteins with an amino acid sequence that has at least 90% identity to a consensus amino acid sequence of proteins and homologs disclosed herein.


Homologs are identified by comparison of amino acid sequence, e.g. manually or by use of a computer-based tool using known homology-based search algorithms such as those commonly known and referred to as BLAST, FASTA, and Smith-Waterman. A local sequence alignment program, e.g. BLAST, can be used to search a database of sequences to find similar sequences, and the summary Expectation value (E-value) used to measure the sequence base similarity. As a protein hit with the best E-value for a particular organism may not necessarily be an ortholog or the only ortholog, a reciprocal query is used in the present invention to filter hit sequences with significant E-values for ortholog identification. The reciprocal query entails search of the significant hits against a database of amino acid sequences from the base organism that are similar to the sequence of the query protein. A hit is a likely ortholog, when the reciprocal query's best hit is the query protein itself or a protein encoded by a duplicated gene after speciation. A further aspect of the invention comprises functional homolog proteins that differ in one or more amino acids from those of disclosed protein as the result of conservative amino acid substitutions, for example substitutions are among: acidic (negatively charged) amino acids such as aspartic acid and glutamic acid; basic (positively charged) amino acids such as arginine, histidine, and lysine; neutral polar amino acids such as glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; neutral nonpolar (hydrophobic) amino acids such as alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; amino acids having aliphatic side chains such as glycine, alanine, valine, leucine, and isoleucine; amino acids having aliphatic-hydroxyl side chains such as serine and threonine; amino acids having amide-containing side chains such as asparagine and glutamine; amino acids having aromatic side chains such as phenylalanine, tyrosine, and tryptophan; amino acids having basic side chains such as lysine, arginine, and histidine; amino acids having sulfur-containing side chains such as cysteine and methionine; naturally conservative amino acids such as valine-leucine, valine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, aspartic acid-glutamic acid, and asparagine-glutamine. A further aspect of the homologs encoded by DNA useful in the transgenic plants of the invention are those proteins that differ from a disclosed protein as the result of deletion or insertion of one or more amino acids in a native sequence.


“Percent identity” describes the extent to which the sequences of DNA or protein segments are invariant throughout a window of alignment of nucleotide or amino acid sequences. An “identity fraction” for a sequence aligned with a reference sequence is the number of identical components which are shared by the sequences, divided by a length of the window of alignment, wherein the length does not include gaps introduced by an alignment algorithm. “Percent identity” (“% identity”) is the identity fraction times 100. The alignment algorithm is preferably a local alignment algorithm, such as BLASTp. As used herein, sequences are “aligned” when the alignment produced by BLASTp has a minimal e-value.


“Pfam” database is a large collection of multiple sequence alignments and hidden Markov models covering many common protein families, e.g. Pfam version 19.0 (December 2005) contains alignments and models for 8183 protein families and is based on the Swissprot 47.0 and SP-TrEMBL 30.0 protein sequence databases. See S. R. Eddy, “Profile Hidden Markov Models”, Bioinformatics 14:755-763, 1998. The Pfam database is currently maintained and updated by the Pfam Consortium. The alignments represent some evolutionary conserved structure that has implications for the protein's function. Profile hidden Markov models (profile HMMs) built from the protein family alignments are useful for automatically recognizing that a new protein belongs to an existing protein family even if the homology by alignment appears to be low.


Protein domains are identified by querying the amino acid sequence of a protein against Hidden Markov Models which characterize protein family domains (“Pfam domains”) using HMMER software, a current version of which is provided in the appended computer listing. A protein domain meeting the gathering cutoff for the alignment of a particular Pfam domain is considered to contain the Pfam domain.


A “Pfam domain module” is a representation of Pfam domains in a protein, in order from N terminus to C terminus. In a Pfam domain module individual Pfam domains are separated by double colons “::”. The order and copy number of the Pfam domains from N to C terminus are attributes of a Pfam domain module. Although the copy number of repetitive domains is important, varying copy number often enables a similar function. Thus, a Pfam domain module with multiple copies of a domain should define an equivalent Pfam domain module with variance in the number of multiple copies. A Pfam domain module is not specific for distance between adjacent domains, but contemplates natural distances and variations in distance that provide equivalent function. The Pfam database contains both narrowly- and broadly-defined domains, leading to identification of overlapping domains on some proteins. A Pfam domain module is characterized by non-overlapping domains. Where there is overlap, the domain having a function that is more closely associated with the function of the protein (based on the E value of the Pfam match) is selected.


Once one DNA is identified as encoding a protein which imparts an enhanced trait when expressed in transgenic plants, other DNA encoding proteins with the same Pfam domain module are identified by querying the amino acid sequence of protein encoded by candidate DNA against the Hidden Markov Models which characterizes the Pfam domains using HMMER software. Candidate proteins meeting the same Pfam domain module are in the protein family and have cognate DNA that is useful in constructing recombinant DNA for the use in the plant cells of this invention. Hidden Markov Model databases for use with HMMER software in identifying DNA expressing protein with a common Pfam domain module for recombinant DNA in the plant cells of this invention are also included in the appended computer listing.


The HMMER software and Pfam databases are version 19.0 and were used to identify known domains in the proteins corresponding to amino acid sequence of SEQ ID NO: 299 through SEQ ID NO: 596. All DNA encoding proteins that have scores higher than the gathering cutoff disclosed in Table 14 by Pfam analysis disclosed herein can be used in recombinant DNA of the plant cells of this invention, e.g. for selecting transgenic plants having enhanced agronomic traits. The relevant Pfams modules for use in this invention, as more specifically disclosed below, are zf-CCCH, PALP, GAF::HisKA::HATPase_c, TPR2::TPR1::TPR2::TPR1::TPR4::TPR2::TPR1, efhand::efhand, Spermine_synth, ELFV_dehydrog_N::ELFV_dehydrog, PFK, PAS3::PAS3::Pkinase, S1, GDC-P, SWIM, B12-binding::Radical_SAM, S1, LRR1::LRR1::LRR1::LRR1::LRR1, Chloroa_b-bind, LRRNT2::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LR R1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::Pkinase, WD40::WD40, YABBY, Ldh1_N::Ldh1_C, Sina, Na_H_antiport1, ParBc, YABBY, Histone, Fe_bilin_red, Tryp_alpha_amyl, Pyr_redox2::Thioredoxin, E2F_TDP, CN_hydrolase, YDG_SRA::Pre-SET::SET, APC8::TPR1::TPR1::TPR1, Ras, tRNA_anti::tRNA-synt2, Auxin_inducible, PGI, S1, Chloroa_b-bind, Bac_globin, Glyco_hydro17, MGS, Spermine_synth, LRRNT2::LRR1::LRR1::LRR1::LRR1::Pkinase, Aa_trans, LRRNT2::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LR R1::LRR1::LRR1::LRR1::LRR1::LRR1::Pkinase_Tyr, Gln-synt_N::Gln-synt_C, SAP18, TPP_enzyme_N::TPP_enzyme_M::TPP_enzyme_C, zf-C3HC4, eIF-1a, RPE65, PBP, Pkinase, AA_permease, F-box::LRR1::LRR2, zf-CCCH::zf-CCCH, Lactamase_B::Flavodoxin1::Flavin_Reduct, Bac_globin, DSPc, adh_short, Tim17, Oxidored_molyb::Mo-co_dimer:Cyt-b5::FAD_binding6::NAD_binding1, ubiquitin::ubiquitin::ubiquitin::ubiquitin, MBD, CXC::CXC, HSF_DNA-bind, Spermine_synth, AP2, Peptidase_S10, PALP, EIN3, Gln-synt_N::Gln-synt_C, 2OG-FeII_Oxy, Glyco_hydro9, GDC-P, B3, PTPA, Acyltransferase, Isochorismatase, FMO-like, Molybdop_Fe4S4::Molybdopterin::Molydop_binding::Fer2_BFD, Lir1, Prismane, Fer2, DEAD::Helicase_C, Molybdop_Fe4S4::Molybdopterin::Molydop_binding::Fer2_BFD, KNOX1::KNOX2::ELK, Glyoxalase, Sad1_UNC, Bac_globin, VPS28, PP2C, Pkinase::efhand::efhand::efhand, LEA3, Peptidase_S10, Pkinase, CBFD_NFYB_HMF, Gln-synt_N::Gln-synt_C, Pyr_redox2::Fer2_BFD::NIR_SIRferr:NIR_SIR, NUDIX::NUDIX, FAD_binding3, GSTN::GST_C, SAM_decarbox, Acyltransferase, NTP_transferase, G-patch, 2OG-FeII_Oxy, Gln-synt_N::Gln-synt_C, AAA::Vps4_C, Histone, Pkinase, TPR1, F-box::Kelch1::Kelch1, Sperminesynth, Bac_globin, Bac_globin, zf-UBR, Homeobox::HALZ, Whirly, NAD_binding1, PTR2, EIN3, 4HBT, adh_short, 2OG-FeII_Oxy, P-II, Myb_DNA-binding::Myb_DNA-binding, DAGK_cat, AP2, MFS1, Chloroa_b-bind, DUF716, zf-D of, CCT, Homeobox::HALZ, Histone, 2OG-FeII_Oxy, Globin, Pyr_redox2::Fer2_BFD::NIR_SIR_ferr:NIR_SIR, Whirly, PsbP, bZIP1, LRRNT2::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LR R1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::Pkinase_Tyr, Phi1, BURP, Sterol_desat, DSPc, SNF5, Acyltransferase, GATase2::Asn_synthase, adh_short, Homeobox:: START, Pkinase, ParBc, SOUL, DNA_photolyase::FAD_binding7, Pkinase_Tyr, NAD_Gly3P_dh_N::NAD_Gly3P_dh_C, Na_H_Exchanger, peroxidase, Oxidored_molyb::Mo-co_dimer::Cyt-b5::FAD_binding6::NAD_binding1, Hexokinase1::Hexokinase2, DUF716, S10_plectin, Thi4, p450, CCT, adh_short, PSI_PSAK, DUF640, Thioredoxin, Globin, Ank::Pkinase, DAGAT, RPE65, Ank::Pkinase, GSHPx, Gln-synt_N::Gln-synt_C, MtN3sly::MtN3_slv, Allene_ox_cyc, IGPD, MBD, CorA, Response_reg, Histone, AAA, Ribosomal_L10e, Pkinase, DUF26::DUF26::Pkinase, p450, mTERF, AA_kinase, PBP, GUN4, Lactamase_B::Flavodoxin1::Rubredoxin, C2, RRM1::RRM1, Histone, Alpha-amylase, HLH, Thioredoxin, Histone_HNS, Myb_DNA-binding::Myb_DNA-binding, Cytochrom_C552, AP2::AP2, MtN3_slv::MtN3_slv, SHMT, ParBc, Mit_rib_S27, Ribosomal_S2, KNOX1::KNOX2::ELK, MFS1, Glyco_transf5::Glycos_transf1, Cellulase, Ribosomal_L10e, Spermine_synth, Glyco_hydro2_N::Glyco_hydro2::Glyco_hydro2_C, TP_methylase, AP2::AP2, Histone, Response_reg::CCT, Histone_HNS, DUF1716, p450, GATA, Pkinase, Sugar_tr, Aa_trans, Pribosyltran, Ribosomal_L10e, HLH, PMSR, DnaJ::DnaJ_CXXCXGXG::DnaJ_C, DUF1005, Glyco_transf5::Glycos_transf1, Spermine_synth, S1::EIF2_alpha, RGS, Na_sulph_symp, S1, MtN3_slv::MtN3_slv, Lactamase_B::Flavin_Reduct, LRRNT2::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LR R1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::LRR1::Pkinase, Chloroa_b-bind, PTR2, Agglutinin, PLATZ, NPH3, Auxin_inducible, PTR2, GAF::HisKA::Response_reg, PsbQ, GSH_synth_ATP, GATase2::Asn_synthase, PHP, FtsJ, DUF6::TPT, Proteasome, PsbW2, Glyco_hydro9, NAD_binding2::6 PGD, S1::EIF2_alpha, Homeobox::START::MEKHLA, S1, Isoamylase_N::Alpha-amylase, E2F_TDP, and Rieske::PaO, for which the databases are included in the appended computer listing.


As used herein “promoter” means regulatory DNA for initializing transcription. A “plant promoter” is a promoter capable of initiating transcription in plant cells whether or not its origin is a plant cell, e.g. is it well known that Agrobacterium promoters are functional in plant cells. Thus, plant promoters include promoter DNA obtained from plants, plant viruses and bacteria such as Agrobacterium and Bradyrhizobium bacteria. Examples of promoters under developmental control include promoters that preferentially initiate transcription in certain tissues, such as leaves, roots, or seeds. Such promoters are referred to as “tissue preferred”. Promoters that initiate transcription only in certain tissues are referred to as “tissue specific”. A “cell type” specific promoter primarily drives expression in certain cell types in one or more organs, for example, vascular cells in roots or leaves. An “inducible” or “repressible” promoter is a promoter which is under environmental control. Examples of environmental conditions that may effect transcription by inducible promoters include anaerobic conditions, or certain chemicals, or the presence of light. Tissue specific, tissue preferred, cell type specific, and inducible promoters constitute the class of “non-constitutive” promoters. A “constitutive” promoter is a promoter which is active under most conditions.


As used herein “operably linked” means the association of two or more DNA fragments in a DNA construct so that the function of one, e.g. protein-encoding DNA, is controlled by the other, e.g. a promoter.


As used herein “expressed” means produced, e.g. a protein is expressed in a plant cell when its cognate DNA is transcribed to mRNA that is translated to the protein.


As used herein a “control plant” means a plant that does not contain the recombinant DNA that expressed a protein that impart an enhanced trait. A control plant is to identify and select a transgenic plant that has an enhance trait. A suitable control plant can be a non-transgenic plant of the parental line used to generate a transgenic plant, i.e. devoid of recombinant DNA. A suitable control plant may in some cases be a progeny of a hemizygous transgenic plant line that is does not contain the recombinant DNA, known as a negative segregant.


As used herein an “enhanced trait” means a characteristic of a transgenic plant that includes, but is not limited to, an enhance agronomic trait characterized by enhanced plant morphology, physiology, growth and development, yield, nutritional enhancement, disease or pest resistance, or environmental or chemical tolerance. In more specific aspects of this invention enhanced trait is selected from group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. In an important aspect of the invention the enhanced trait is enhanced yield including increased yield under non-stress conditions and increased yield under environmental stress conditions. Stress conditions may include, for example, drought, shade, fungal disease, viral disease, bacterial disease, insect infestation, nematode infestation, cold temperature exposure, heat exposure, osmotic stress, reduced nitrogen nutrient availability, reduced phosphorus nutrient availability and high plant density. “Yield” can be affected by many properties including without limitation, plant height, pod number, pod position on the plant, number of internodes, incidence of pod shatter, grain size, efficiency of nodulation and nitrogen fixation, efficiency of nutrient assimilation, resistance to biotic and abiotic stress, carbon assimilation, plant architecture, resistance to lodging, percent seed germination, seedling vigor, and juvenile traits. Yield can also be affected by efficiency of germination (including germination in stressed conditions), growth rate (including growth rate in stressed conditions), ear number, seed number per ear, seed size, composition of seed (starch, oil, protein) and characteristics of seed fill.


Increased yield of a transgenic plant of the present invention can be measured in a number of ways, including test weight, seed number per plant, seed weight, seed number per unit area (i.e. seeds, or weight of seeds, per acre), bushels per acre, tonnes per acre, tons per acre, kilo per hectare. For example, maize yield may be measured as production of shelled corn kernels per unit of production area, for example in bushels per acre or metric tons per hectare, often reported on a moisture adjusted basis, for example at 15.5 percent moisture. Increased yield may result from improved utilization of key biochemical compounds, such as nitrogen, phosphorous and carbohydrate, or from improved responses to environmental stresses, such as cold, heat, drought, salt, and attack by pests or pathogens. Recombinant DNA used in this invention can also be used to provide plants having improved growth and development, and ultimately increased yield, as the result of modified expression of plant growth regulators or modification of cell cycle or photosynthesis pathways. Also of interest is the generation of transgenic plants that demonstrate enhanced yield with respect to a seed component that may or may not correspond to an increase in overall plant yield. Such properties include enhancements in seed oil, seed molecules such as tocopherol, protein and starch, or oil particular oil components as may be manifest by an alterations in the ratios of seed components.


A subset of the nucleic molecules of this invention includes fragments of the disclosed recombinant DNA consisting of oligonucleotides of at least 15, preferably at least 16 or 17, more preferably at least 18 or 19, and even more preferably at least 20 or more, consecutive nucleotides. Such oligonucleotides are fragments of the larger molecules having a sequence selected from the group consisting of SEQ ID NO:1 through SEQ ID NO: 298, and find use, for example as probes and primers for detection of the polynucleotides of the present invention.


In some embodiments of the invention a constitutively active mutant, e.g. SEQ ID NO: 203, is constructed to achieve the desired effect. In other embodiments of the invention, a dominant negative gene is constructed to adversely affect the normal, wild-type gene product within the same cell.


DNA constructs are assembled using methods well known to persons of ordinary skill in the art and typically comprise a promoter operably linked to DNA, the expression of which provides the enhanced agronomic trait. Other construct components may include additional regulatory elements, such as 5′ leasders and introns for enhancing transcription, 3′ untranslated regions (such as polyadenylation signals and sites), DNA for transit or signal peptides.


Numerous promoters that are active in plant cells have been described in the literature. These include promoters present in plant genomes as well as promoters from other sources, including nopaline synthase (NOS) promoter and octopine synthase (OCS) promoters carried on tumor-inducing plasmids of Agrobacterium tumefaciens and the CaMV35S promoters from the cauliflower mosaic virus as disclosed in U.S. Pat. Nos. 5,164,316 and 5,322,938. Useful promoters derived from plant genes are found in U.S. Pat. No. 5,641,876, which discloses a rice actin promoter, U.S. Pat. No. 7,151,204, which discloses a maize chloroplast aldolase promoter and a maize aldolase (FDA) promoter, and U.S. Patent Application Publication 2003/0131377 A1, which discloses a maize nicotianamine synthase promoter, all of which are incorporated herein by reference. These and numerous other promoters that function in plant cells are known to those skilled in the art and available for use in recombinant polynucleotides of the present invention to provide for expression of desired genes in transgenic plant cells.


In other aspects of the invention, preferential expression in plant green tissues is desired. Promoters of interest for such uses include those from genes such as Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (Rubisco) small subunit (Fischhoff et al. (1992) Plant Mol Biol. 20:81-93), aldolase and pyruvate orthophosphate dikinase (PPDK) (Taniguchi et al. (2000) Plant Cell Physiol. 41(1):42-48).


Furthermore, the promoters may be altered to contain multiple “enhancer sequences” to assist in elevating gene expression. Such enhancers are known in the art. By including an enhancer sequence with such constructs, the expression of the selected protein may be enhanced. These enhancers often are found 5′ to the start of transcription in a promoter that functions in eukaryotic cells, but can often be inserted upstream (5′) or downstream (3′) to the coding sequence. In some instances, these 5′ enhancing elements are introns. Particularly useful as enhancers are the 5′ introns of the rice actin 1 (see U.S. Pat. No. 5,641,876) and rice actin 2 genes, the maize alcohol dehydrogenase gene intron, the maize heat shock protein 70 gene intron (U.S. Pat. No. 5,593,874) and the maize shrunken 1 gene.


In other aspects of the invention, sufficient expression in plant seed tissues is desired to affect improvements in seed composition. Exemplary promoters for use for seed composition modification include promoters from seed genes such as napin (U.S. Pat. No. 5,420,034), maize L3 oleosin (U.S. Pat. No. 6,433,252), zein Z27 (Russell et al. (1997) Transgenic Res. 6(2):157-166), globulin 1 (Belanger et al (1991) Genetics 129:863-872), glutelin 1 (Russell (1997) supra), and peroxiredoxin antioxidant (Per1) (Stacy et al. (1996) Plant Mol Biol. 31(6):1205-1216).


Recombinant DNA constructs prepared in accordance with the invention will also generally include a 3′ element that typically contains a polyadenylation signal and site. Well-known 3′ elements include those from Agrobacterium tumefaciens genes such as nos 3′, tml 3, tmr 3′, tins 3′, ocs 3′, tr7 3′, for example disclosed in U.S. Pat. No. 6,090,627, incorporated herein by reference; 3′ elements from plant genes such as wheat (Triticum aesevitum) heat shock protein 17 (Hsp17 3′), a wheat ubiquitin gene, a wheat fructose-1,6-biphosphatase gene, a rice glutelin gene, a rice lactate dehydrogenase gene and a rice beta-tubulin gene, all of which are disclosed in U.S. published patent application 2002/0192813 A1, incorporated herein by reference; and the pea (Pisum sativum) ribulose biphosphate carboxylase gene (rbs 3′), and 3′ elements from the genes within the host plant.


Constructs and vectors may also include a transit peptide for targeting of a gene to a plant organelle, particularly to a chloroplast, leucoplast or other plastid organelle. For descriptions of the use of chloroplast transit peptides see U.S. Pat. No. 5,188,642 and U.S. Pat. No. 5,728,925, incorporated herein by reference. For description of the transit peptide region of an Arabidopsis EPSPS gene useful in the present invention, see Klee, H. J. et al (MGG (1987) 210:437-442).


Transgenic plants comprising or derived from plant cells of this invention transformed with recombinant DNA construct can be further enhanced with stacked traits, e.g. a crop plant having an enhanced trait resulting from expression of DNA disclosed herein in combination with herbicide and/or pest resistance traits. For example, genes of the current invention can be stacked with other traits of agronomic interest, such as a trait providing herbicide resistance, or insect resistance, such as using a gene from Bacillus thuringensis to provide resistance against lepidopteran, coliopteran, homopteran, hemiopteran, and other insects. Herbicides for which transgenic plant tolerance has been demonstrated and the method of the present invention can be applied include, but are not limited to, glyphosate, dicamba, glufosinate, sulfonylurea, bromoxynil and norflurazon herbicides. Polynucleotide molecules encoding proteins involved in herbicide tolerance are well-known in the art and include, but are not limited to, a polynucleotide molecule encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) disclosed in U.S. Pat. Nos. 5,094,945; 5,627,061; 5,633,435 and 6,040,497 for imparting glyphosate tolerance; polynucleotide molecules encoding a glyphosate oxidoreductase (GOX) disclosed in U.S. Pat. No. 5,463,175 and a glyphosate-N-acetyl transferase (GAT) disclosed in U.S. Patent Application publication 2003/0083480 A1 also for imparting glyphosate tolerance; dicamba monooxygenase disclosed in U.S. Patent Application publication 2003/0135879 A1 for imparting dicamba tolerance; a polynucleotide molecule encoding bromoxynil nitrilase (Bxn) disclosed in U.S. Pat. No. 4,810,648 for imparting bromoxynil tolerance; a polynucleotide molecule encoding phytoene desaturase (crtl) described in Misawa et al, (1993) Plant J. 4:833-840 and in Misawa et al, (1994) Plant J. 6:481-489 for norflurazon tolerance; a polynucleotide molecule encoding acetohydroxyacid synthase (AHAS, aka ALS) described in Sathasiivan et al. (1990) Nucl. Acids Res. 18:2188-2193 for imparting tolerance to sulfonylurea herbicides; polynucleotide molecules known as bar genes disclosed in DeBlock, et al. (1987) EMBO J. 6:2513-2519 for imparting glufosinate and bialaphos tolerance; polynucleotide molecules disclosed in U.S. Patent Application Publication 2003/010609 A1 for imparting N-amino methyl phosphonic acid tolerance; polynucleotide molecules disclosed in U.S. Pat. No. 6,107,549 for impartinig pyridine herbicide resistance; molecules and methods for imparting tolerance to multiple herbicides such as glyphosate, atrazine, ALS inhibitors, isoxoflutole and glufosinate herbicides are disclosed in U.S. Pat. No. 6,376,754 and U.S. Patent Application Publication 2002/0112260, all of said U.S. Patents and Patent Application Publications are incorporated herein by reference. Molecules and methods for imparting insect/nematode/virus resistance are disclosed in U.S. Pat. Nos. 5,250,515; 5,880,275; 6,506,599; 5,986,175 and U.S. Patent Application Publication 2003/0150017 A1, all of which are incorporated herein by reference.


Plant Cell Transformation Methods

Numerous methods for transforming chromosomes in a plant cell nucleus with recombinant DNA are known in the art and are used in methods of preparing a transgenic plant cell nucleus cell, and plant. Two effective methods for such transformation are Agrobacterium-mediated transformation and microprojectile bombardment. Microprojectile bombardment methods are illustrated in U.S. Pat. Nos. 5,015,580 (soybean); 5,550,318 (corn); 5,538,880 (corn); 5,914,451 (soybean); 6,160,208 (corn); 6,399,861 (corn); 6,153,812 (wheat) and 6,365,807 (rice) and Agrobacterium-mediated transformation is described in U.S. Pat. Nos. 5,159,135 (cotton); 5,824,877 (soybean); 5,463,174 (canola); 5,591,616 (corn); 6,384,301 (soybean), 7,026,528 (wheat) and 6,329,571 (rice), all of which are incorporated herein by reference. Transformation of plant material is practiced in tissue culture on a nutrient media, i.e. a mixture of nutrients that will allow cells to grow in vitro. Recipient cell targets include, but are not limited to, meristem cells, hypocotyls, calli, immature embryos and gametic cells such as microspores, pollen, sperm and egg cells. Callus may be initiated from tissue sources including, but not limited to, immature embryos, hypocotyls, seedling apical meristems, microspores and the like. Cells containing a transgenic nucleus are grown into transgenic plants.


In addition to direct transformation of a plant material with a recombinant DNA, a transgenic plant cell nucleus can be prepared by crossing a first plant having cells with a transgenic nucleus with recombinant DNA with a second plant lacking the trangenci nucleus. For example, recombinant DNA can be introduced into a nucleus from a first plant line that is amenable to transformation to transgenic nucleus in cells that are grown into a transgenic plant which can be crossed with a second plant line to introgress the recombinant DNA into the second plant line. A transgenic plant with recombinant DNA providing an enhanced trait, e.g. enhanced yield, can be crossed with transgenic plant line having other recombinant DNA that confers another trait, for example herbicide resistance or pest resistance, to produce progeny plants having recombinant DNA that confers both traits. Typically, in such breeding for combining traits the transgenic plant donating the additional trait is a male line and the transgenic plant carrying the base traits is the female line. The progeny of this cross will segregate such that some of the plants will carry the DNA for both parental traits and some will carry DNA for one parental trait; such plants can be identified by markers associated with parental recombinant DNA, e.g. marker identification by analysis for recombinant DNA or, in the case where a selectable marker is linked to the recombinant, by application of the selecting agent such as a herbicide for use with a herbicide tolerance marker, or by selection for the enhanced trait. Progeny plants carrying DNA for both parental traits can be crossed back into the female parent line multiple times, for example usually 6 to 8 generations, to produce a progeny plant with substantially the same genotype as one original transgenic parental line but for the recombinant DNA of the other transgenic parental line


In the practice of transformation DNA is typically introduced into only a small percentage of target plant cells in any one transformation experiment. Marker genes are used to provide an efficient system for identification of those cells that are stably transformed by receiving and integrating a recombinant DNA molecule into their genomes. Preferred marker genes provide selective markers which confer resistance to a selective agent, such as an antibiotic or a herbicide. Any of the herbicides to which plants of this invention may be resistant are useful agents for selective markers. Potentially transformed cells are exposed to the selective agent. In the population of surviving cells will be those cells where, generally, the resistance-conferring gene is integrated and expressed at sufficient levels to permit cell survival. Cells may be tested further to confirm stable integration of the exogenous DNA.


Commonly used selective marker genes include those conferring resistance to antibiotics such as kanamycin and paromomycin (nptII), hygromycin B (aph IV), spectinomycin (aadA) and gentamycin (aac3 and aacC4) or resistance to herbicides such as glufosinate (bar or pat), dicamba (DMO) and glyphosate (aroA or EPSPS). Examples of such selectable markers are illustrated in U.S. Pat. Nos. 5,550,318; 5,633,435; 5,780,708 and 6,118,047, all of which are incorporated herein by reference. Selectable markers which provide an ability to visually identify transformants can also be employed, for example, a gene expressing a colored or fluorescent protein such as a luciferase or green fluorescent protein (GFP) or a gene expressing a beta-glucuronidase or uidA gene (GUS) for which various chromogenic substrates are known.


Plant cells that survive exposure to the selective agent, or plant cells that have been scored positive in a screening assay, may be cultured in regeneration media and allowed to mature into plants. Developing plantlets regenerated from transformed plant cells can be transferred to plant growth mix, and hardened off, for example, in an environmentally controlled chamber at about 85% relative humidity, 600 ppm CO2, and 25-250 microeinsteins m−2 s−1 of light, prior to transfer to a greenhouse or growth chamber for maturation. Plants are regenerated from about 6 weeks to 10 months after a transformant is identified, depending on the initial tissue, and plant species. Plants may be pollinated using conventional plant breeding methods known to those of skill in the art and seed produced, for example self-pollination is commonly used with transgenic corn. The regenerated transformed plant or its progeny seed or plants can be tested for expression of the recombinant DNA and selected for the presence of enhanced agronomic trait.


Transgenic Plants and Seeds

Transgenic plants derived from the plant cells of this invention are grown to generate transgenic plants having an enhanced trait as compared to a control plant and produce transgenic seed and pollen of this invention. Such transgenic plants with enhanced traits are identified by selection of transformed plants or progeny seed for the enhanced trait. For efficiency a selection method is designed to evaluate multiple transgenic plants (events) comprising the recombinant DNA, for example multiple plants from 2 to 20 or more transgenic events. Transgenic plants grown from transgenic seed provided herein have enhanced agronomic traits that contribute to increased yield or other trait that provides increased plant value, including, for example, improved seed quality. Of particular interest are plants having enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.


Table 2 provides a list of protein encoding DNA (“genes”) that are useful as DNA segament in a recombinant DNA construct for production of transgenic plants with enhanced agronomic trait, the elements of Table 2 are described by reference to:


“PEP SEQ ID NO” identifies an amino acid sequence from SEQ ID NO: 299 to 596.


“NUC SEQ ID NO” identifies a DNA sequence from SEQ ID NO:1 to 298.


“BV id” is a reference to the identifying number of base vectors in Table 3 used for construction of the transformation vectors of the recombinant DNA. Construction of plant transformation constructs is illustrated in Example 1.


“Gene Name” denotes a common name for protein encoded by the recombinant DNA.


“Annotation” refers to a description of the top hit protein obtained from an amino acid sequence query of each PEP SEQ ID NO to GenBank database of the National Center for Biotechnology Information (ncbi). More particularly, “gi” is the GenBank ID number for the top BLAST hit;


“% id” refers to the percentage of identically matched amino acid residues along the length of the portion of the sequences which is aligned by BLAST (−F T) between the sequence of interest provided herein and the hit sequence in GenBank.

















TABLE 2





NUC
PEP









SEQ
SEQ


ID
ID

BV


%
GenBank


NO
NO
Gene ID
ID
Gene Name
e-value
id
ID
Annotation























1
299
PHE0007721_18998
21
Corn Homeotic
1.00E−152
77
123183
gb|AAP76321.1|homeobox






protein knotted-1



transcription factor KNOTTED1










[Zea mays]


2
300
PHE0007756_18009
33

Arabidopsis 9-cis-

0
90
15231856
dbj|BAB01336.1|9-cis-






epoxycarotenoid



epoxycarotenoid dioxygenase






dioxygenase



[Arabidopsis thaliana]


3
301
PHE0006007_18714
30

Arabidopsis nodulin

1.00E−105
86
79607902
ref|NP_974371.2|unknown






MtN21 family protein



protein [Arabidopsis thaliana]


4
302
PHE0004988_15925
13

Arabidopsis

0
100
18412567
gb|AAN71945.1|transport






transport inhibitor



inhibitor response TIR1,






response 1



AtFBL1 protein [Arabidopsis











thaliana]



5
303
PHE0007140_21771
30

Arabidopsis nodulin

1.00E−121
92
18421965
gb|AAM61405.1|MtN3 like






MtN3 like protein-4



[Arabidopsis thaliana]


5
303
PHE0007140_22049
13

Arabidopsis nodulin

1.00E−121
92
18421965
gb|AAM61405.1|MtN3 like






MtN3 like protein-4



[Arabidopsis thaliana]


6
304
PHE0006823_16403
13

Arabidopsis class 2

3.00E−85
100
15228313
gb|AAM65188.1|Non-






non-symbiotic



symbiotic hemoglobin Hb2






hemoglobin



[Arabidopsis thaliana]


7
305
PHE0007440_22555
18
Corn putative GTP-
1.00E−107
91
115462975
gb|AAS98506.1|GTP-binding






binding protein



protein Rab11 [Oryza sativa






Rab11



(japonica cultivar-group)]


8
306
PHE0000206_22432
9
Corn CDPK kinase
0
87
115450483
sp|P53684|Calcium-dependent






domain



protein kinase, isoform 11










(CDPK 11) [Oryza sativa










(japonica cultivar-group)]


9
307
PHE0000598_16824
9
Corn B1 regulatory
0
91
22195
emb|CAA40544.1|regulatory






protein



protein [Zea mays]


10
308
PHE0007590_17883
8

E. Coli ribosomal

7.00E−33
56
118740922
ref|ZP_01588962.1|ribosomal






protein S1 domain 3



protein S1 [Enterobacter sp.










638]


11
309
PHE0007588_17881
8

E. Coli RNAse E S1

2.00E−45
98
52695516
pdb|1SMX|the S1 domain of






domain



Rnase E from E. Coli (Native)


12
310
PHE0007592_17885
8
Yeast pre-mRNA
1.00E−40
98
6320850
gb|AAB64546.1|pre-mRNA






splicing factor RNA



splicing factor RNA helicase






helicase S1 domain



[Saccharomyces cerevisiae]


13
311
PHE0007587_17880
8

E. coli

7.00E−35
100
124525928
ref|ZP_01697933.1|






Polyribonucleotide



Polyribonucleotide






nucleotidyltransferase



nucleotidyltransferase










[Escherichia coli B]


14
312
PHE0007624_17923
8

E. Coli translation

8.00E−34
100
15800747
ref|NP_286761.1|translation






initiation factor IF-1



initiation factor IF-1










[Escherichia coli O157:H7










EDL933]


15
313
PHE0007591_17884
8

E. Coli ribosomal

2.00E−20
51
118068082
ref|ZP_01536336.1|ribosomal






protein S1 domain 5



protein S1 [Serratia











proteamaculans 568]



16
314
PHE0007623_17922
8

Arabidopsis N-

1.00E−172
100
18401429
gb|AAM63266.1|putative






carbamoylputrescine



nitrilase [Arabidopsis thaliana]






amidohydrolase


17
315
PHE0007583_17871
6
Rice MtN3 homolog
1.00E−130
100
125526770
gb|EAY74884.1|hypothetical










protein OsI_002731 [Oryza











sativa (indica cultivar-group)]



18
316
PHE0006618_16146
13
Corn putative
4.00E−49
95
115442401
dbj|BAB63622.1|putative 40S






ribosomal protein



ribosomal protein S10 [Oryza






S10




sativa (japonica cultivar-











group)]


19
317
PHE0006791_16374
9

Arabidopsis

1.00E−120
83
15231674
sp|Q9LX31|GUN4_ARATH






GUN4_ARATH



Tetrapyrrole-binding protein,






Tetrapyrrole-binding



chloroplast precursor






protein



[Arabidopsis thaliana]


20
318
PHE0007620_17918
13

Arabidopsis

0
96
15223971
dbj|BAF01533.1|thylakoid-






thylakoid ascorbate



bound ascorbate peroxidase






peroxidase



[Arabidopsis thaliana]


21
319
PHE0006659_16195
13
Soy hypothetical
1.00E−112
49
125540608
gb|EAY87003.1|hypothetical






protein



protein OsI_008236 [Oryza











sativa (indica cultivar-group)]



22
320
PHE0006662_16198
13

Ralstonia

1.00E−06
38
114582111
ref|XP_001161638.1|







metallidurans




hypothetical protein isoform 1






hypothetical protein



[Pan troglodytes]


23
321
PHE0006274_15867
13
Corn Transcription
0
78
115487438
gb|ABA96515.1|Transcription






factor E2Fb



factor E2F [Oryza sativa










(japonica cultivar-group)]


24
322
PHE0006637_16166
14
Corn ABI1 homolog
1.00E−145
78
115437928
ref|NP_001043415.1|Os01g0583100










[Oryza sativa (japonica










cultivar-group)]


25
323
PHE0006926_16815
13
Corn putative
1.00E−84
81
115440323
dbj|BAB63580.1|putative






vacuolar protein



vacuolar protein sorting 28






sorting-associated



[Oryza sativa (japonica






protein



cultivar-group)]


26
324
PHE0006821_16402
13
Corn 1-
1.00E−176
79
115448975
dbj|BAD16875.1|1-






aminocyclopropane-



aminocyclopropane-1-






1-carboxylate



carboxylate deaminase [Oryza






deaminase




sativa (japonica cultivar-











group)]


27
325
PHE0007687_18057
29

Arabidopsis

0
100
21536828
gb|AAM61160.1|cytochrome






cytochrome P450



P450 homolog [Arabidopsis











thaliana]



27
325
PHE0007687_18200
13

Arabidopsis

0
100
21536828
gb|AAM61160.1|cytochrome






cytochrome P450



P450 homolog, [Arabidopsis











thaliana]



28
326
PHE0007642_17999
29

Arabidopsis Non-

0
94
15231245
sp|O48963|Phototropin-1






phototropic



(Root phototropism protein 1)






hypocotyl protein 1



[Arabidopsis thaliana]


29
327
PHE0007677_18052
29

Arabidopsis

0
100
15239022
ref|NP_196694.1|oxidoreductase






oxidoreductase



[Arabidopsis thaliana]


29
327
PHE0007677_18179
13

Arabidopsis

0
100
15239022
ref|NP_196694.1|oxidoreductase






oxidoreductase



[Arabidopsis thaliana]


30
328
PHE0007644_18001
29

Arabidopsis receptor

0
88
15220455
ref|NP_176918.1|protein






protein kinase



serine/threonine kinase










[Arabidopsis thaliana]


30
328
PHE0007644_18169
13

Arabidopsis receptor

0
88
15220455
ref|NP_176918.1|protein






protein kinase



serine/threonine kinase










[Arabidopsis thaliana]


31
329
PHE0007640_17992
29
Soy magnesium
1.00E−165
80
92889086
gb|ABE89684.1|Mg2+






transporter mrs2-1-



transporter protein, CorA-like






like



[Medicago truncatula]


32
330
PHE0007678_18053
29

Arabidopsis acyl-

1.00E−107
100
18399594
ref|NP_564457.1|acyl-CoA






CoA thioesterase



thioesterase [Arabidopsis











thaliana]



32
330
PHE0007678_18180
13

Arabidopsis acyl-

1.00E−107
100
18399594
ref|NP_564457.1|acyl-CoA






CoA thioesterase



thioesterase [Arabidopsis











thaliana]



33
331
PHE0007649_18006
29

Arabidopsis putative

0
99
42566072
ref|NP_191542.2|protein






ankyrin protein



kinase/threonine kinase/






kinase



protein-tyrosine kinase










[Arabidopsis thaliana]


34
332
PHE0006726_16298
25

Arabidopsis putative

0
97
15224586
gb|AAC20725.1|putative






ARP2/3 protein



ARP2/3 protein complex






complex subunit p41



subunit p41 [Arabidopsis











thaliana]



35
333
PHE0006218_8776
25

Arabidopsis

0
85
18405775
ref|NP_565954.1|disulfide






thioredoxin-disulfide



oxidoreductase/thioredoxin-






reductase



disulfide reductase










[Arabidopsis thaliana]


36
334
PHE0006657_16192
13
Corn putative ATP-
2.00E−83
71
115462871
gb|AAV31386.1|putative ATP-






binding protein



binding protein [Oryza sativa










(japonica cultivar-group)]


37
335
PHE0002554_17876
13
Corn Chlorophyll
1.00E−158
95
2326947
emb|CAA90681.1|Chlorophyll






a/b-binding protein



a/b-binding protein CP29






CP29 precursor



precursor [Zea mays]


37
335
PHE0002554_23104
9
Corn Chlorophyll
1.00E−158
95
2326947
emb|CAA90681.1|Chlorophyll






a/b-binding protein



a/b-binding protein CP29






CP29 precursor



precursor [Zea mays]


38
336
PHE0006807_16388
13
Corn transcription
1.00E−140
64
115484281
gb|ABG22372.1|CCT motif






factor APRR9 like



family protein [Oryza sativa










(japonica cultivar-group)]


39
337
PHE0003359_8487
9
Corn dwf4-like
0
86
60677681
dbj|BAD90972.1|cytochrome






protein



P450 [Oryza sativa (japonica










cultivar-group)]


40
338
PHE0006542_15765
9

Arabidopsis

1.00E−149
100
15234942
ref|NP_194243.1|protein-






methionine sulfoxide



methionine-S-oxide reductase






reductase



[Arabidopsis thaliana]


41
339
PHE0007622_17921
8

Arabidopsis

0
100
18419941
gb|AAF01311.1|spermine






spermine synthase



synthase [Arabidopsis











thaliana]



42
340
PHE0007630_17956
3
Corn GA3ox1
1.00E−151
75
125544881
gb|EAY91020.1|hypothetical










protein OsI_012253 [Oryza











sativa (indica cultivar-group)]



43
341
PHE0007593_17886
8
Corn spermidine
1.00E−169
92
115471679
dbj|BAD30581.1|spermidine






synthase



synthase 1 [Oryza sativa










(japonica cultivar-group)]


44
342
PHE0006475_15588
9
Arbidopsis
9.00E−89
100
42571459
ref|NP_973820.1|calcium ion






PsbQ_like



binding [Arabidopsis thaliana]


45
343
PHE0007619_17915
13
Soy methyl-CpG-
1.00E−44
43
18394229
ref|NP_563971.1|MBD10; DNA






binding domain-



binding [Arabidopsis thaliana]






containing protein


46
344
PHE0006846_16447
13
Soy cuticle protein
0
70
9758349
dbj|BAB08850.1|lipid transfer










protein; glossy1 homolog










[Arabidopsis thaliana]


47
345
PHE0007584_17874
13

Arabidopsis methyl-

1.00E−124
65
18394229
ref|NP_563971.1|MBD10;






CpG-binding



DNA binding [Arabidopsis






domain-containing




thaliana]







protein


48
346
PHE0003695_17913
17

Arabidopsis Erecta

0
95
15225286
gb|AAC49302.1|ERECTA,










receptor protein kinase










[Arabidopsis thaliana]


48
346
PHE0011554_23650
32

Arabidopsis Erecta

0
95
15225286
gb|AAC49302.1|ERECTA,










receptor protein kinase










[Arabidopsis thaliana]


49
347
PHE0003695_17879
8

Arabidopsis Erecta

0
95
15225286
gb|AAK59615.1|receptor










protein kinase, ERECTA










[Arabidopsis thaliana]


49
347
PHE0003695_23448
22

Arabidopsis Erecta

0
95
15225286
gb|AAK59615.1|receptor










protein kinase, ERECTA










[Arabidopsis thaliana]


50
348
PHE0006936_16828
29
Soy GNS1/SUR4
2.00E−91
66
92868497
gb|ABE78502.1|GNS1/SUR4






membrane protein



membrane protein [Medicago











truncatula]



51
349
PHE0007645_18002
29
Soy DNA binding
2.00E−26
32
21617964
gb|AAM67014.1|DNA binding






protein



protein-like [Arabidopsis











thaliana]



52
350
PHE0006784_16366
6
Corn cysteine
1.00E−165
93
2829688
emb|CAA59798.1|O-






synthase



acetylserine (thiol) lyase;










cysteine synthase [Zea mays]


53
351
PHE0006171_16491
13
Sorghum bicolor
6.00E−91
86
115463027
ref|NP_001055113.1|Os05g0295800






Glyoxalase



[Oryza sativa (japonica










cultivar-group)]


54
352
PHE0006868_16682
13

Arabidopsis

3.00E−05
100
21592593
gb|AAM64542.1|unknown






ME01451



[Arabidopsis thaliana]


55
353
PHE0006906_16796
13
Corn 20S
1.00E−121
96
115480019
dbj|BAA28276.1|beta 6






proteasome subunit



subunit of 20S proteasome






beta-6



[Oryza sativa (japonica










cultivar-group)]


56
354
PHE0006649_16180
13
Wheat putative DRE
5.00E−62
64
60547461
gb|AAX23704.1|HvCBF7






binding factor 2



[Hordeum vulgare subsp.











vulgare]



57
355
PHE0006809_16390
13

E. Coli hydroxylamine

0
97
89107724
ref|NP_415394.4|






reductase



hydroxylamine reductase










[Escherichia coli K12]


58
356
PHE0006918_16808
13
Corn putative
0
82
125571037
gb|EAZ12552.1|hypothetical






dihydropyrimidine



protein OsJ_002377 [Oryza






dehydrogenase




sativa (japonica cultivar-











group)]


59
357
PHE0007813_18219
6
Rice MtN3 homolog
1.00E−119
86
115445683
dbj|BAD23335.1|putative










NEC1 [Oryza sativa (japonica










cultivar-group)]


60
358
PHE0007650_18175
13

Arabidopsis Kin17

0
83
18405389
AAD10649.1|Kin17 protein






protein



[Arabidopsis thaliana]


61
359
PHE0006825_16405
13

E. Coli cytochrome c

0
100
15834306
ref|ZP_01699144.1|Nitrite






nitrite reductase



reductase [Escherichia coli B]


62
360
PHE0006964_16864
9
Corn asparaginyl-
0
84
115436616
dbj|BAB61140.1|putative






tRNA synthetase



asparagine-tRNA ligase [Oryza











sativa (japonica cultivar-











group)]


62
360
PHE0006964_16865
13
Corn asparaginyl-
0
84
115436616
dbj|BAB61140.1|putative






tRNA synthetase



asparagine-tRNA ligase [Oryza











sativa (japonica cultivar-











group)]


63
361
PHE0008105_18407
13
Yeast transcriptional
1.00E−116
88
6324934
sp|Q02516|Transcriptional






activator HAP5



activator HAP5










[Saccharomyces cerevisiae]


63
361
PHE0008105_18733
30
Yeast transcriptional
1.00E−116
88
6324934
sp|Q02516|Transcriptional






activator HAP5



activator HAP5










[Saccharomyces cerevisiae]


64
362
PHE0006630_16159
13
Corn malate
0
92
126888
sp|P15719|MDHP_maize






dehydrogenase



Malate dehydrogenase [NADP]


65
363
PHE0006966_16868
29

Arabidopsis

0
95
18413170
ref|NP_567341.1|Cryptochrome






Cryptochrome 1



1 [Arabidopsis thaliana]


66
364
PHE0008698_24421
3
Corn expressed
9.00E−06
38
115484935
gb|AAX96767.1|expressed






protein



protein [Oryza sativa (japonica










cultivar-group)]


67
365
PHE0006608_16123
25
Soy glutathione
2.00E−82
90
21068666
emb|CAD31839.1|putative






peroxidase



phospholipid hydroperoxide










glutathione peroxidase [Cicer











arietinum]



68
366
PHE0010462_21459
na

Deinococcus

1.00E−155
86
15805358
ref|NP_294052.1|MutT/nudix







radiodurans




family protein [Deinococcus






nudixhydrolase




radiodurans R1]



69
367
PHE0009211_21774
30

Arabidopsis

5.00E−50
89
15236198
ref|NP_195205.1|unknown






PF02519-auxin



protein [Arabidopsis thaliana]






inducible-3


70
368
PHE0006256_8775
27

Arabidopsis 9-cis-

0
92
15231856
dbj|BAB01336.1|9-cis-






epoxycarotenoid



epoxycarotenoid dioxygenase






dioxygenase



[Arabidopsis thaliana]


71
369
PHE0006989_16918
29

Arabidopsis putative

0
79
15231840
dbj|BAB01042.1|protein






protein kinase



kinase [Arabidopsis thaliana]


72
370
PHE0007649_18166
13

Arabidopsis putative

0
100
42566072
ref|NP_191542.2|protein






ankyrin protein



kinase/serine/threonine kinase/






kinase



tyrosine kinase [Arabidopsis











thaliana]



73
371
PHE0006708_16264
29
Soy DNA helicase
0
89
3097266
emb|CAA76677.1|translation






45



initiation factor [Pisum sativum]


74
372
PHE0001117_19094
4
Corn transcription
1.00E−101
84
125527033
gb|EAY75147.1|hypothetical






factor G1543-like



protein OsI_002994 [Oryza











sativa (indica cultivar-group)]



74
372
PHE0001117_19095
9
Corn transcription
1.00E−101
84
125527033
gb|EAY75147.1|hypothetical






factor G1543-like



protein OsI_002994 [Oryza











sativa (indica cultivar-group)]



75
373
PHE0006266_8814
9
corn
1.00E−164
100
49472889
gb|AAT66259.1|






phytochromobilin



phytochromobilin synthase






synthase



[Zea mays]


75
373
PHE0006266_15483
4
corn
1.00E−164
100
49472889
gb|AAT66259.1|






phytochromobilin



phytochromobilin synthase






synthase



[Zea mays]


76
374
PHE0006795_16378
13

E. Coli pH-

1.00E−172
82
16128013
ref|NP_414560.1|pH-






dependent



dependent sodium/proton






sodium/proton



antiporter [Escherichia coli






antiporter



K12]


77
375
PHE0006956_16853
13

Agrobacterium

0
98
15891062
ref|NP_356734.1|hypothetical






nitrate reductase



protein AGR_L_1895










[Agrobacterium tumefaciens










str. C58]


78
376
PHE0006859_16873
9
Corn bHLH protein
2.00E−31
92
115451023
gb|EAZ25749.1|hypothetical






family



protein OsJ_009232 [Oryza











sativa (japonica cultivar-











group)]


79
377
PHE0006824_16404
13
Corn Nonlegume
6.00E−86
98
3913789
gb|AAA19576.1|haemoglobin






hemoglobin-like



apoprotein [Hordeum vulgare]


80
378
PHE0006812_16393
13

Nostoc 2-on-2

1.00E−61
100
23130505
ref|ZP_00112318.1|Truncated






hemoglobin



hemoglobins [Nostoc











punctiforme PCC 73102]



81
379
PHE0006815_16396
13
Rice Trb-like
2.00E−98
100
115468746
dbj|BAD32857.1|putative 2-










on-2 hemoglobin [Oryza sativa










(japonica cultivar-group)]


82
380
PHE0006806_16387
13
Corn Trb-like
7.00E−80
83
14165165
gb|AAK55410.1|AF376063_12-










on-2 hemoglobin [Hordeum











vulgare]



83
381
PHE0006624_16153
13
Corn putative
0
98
3342802
gb|AAC27703.1|putative






cytosolic 6-



cytosolic 6-phosphogluconate






phosphogluconate



dehydrogenase [Zea mays]






dehydrogenase


84
382
PHE0005006_15823
13
Corn chlorophyll a/b-
1.00E−112
80
115458738
gb|EAY94374.1|hypothetical






binding apoprotein



protein OsI_015607 [Oryza






CP24 precursor




sativa (indica cultivar-group)]



85
383
PHE0003991_16771
13
Corn










AfMONFEED000559






Protamine P1


86
384
PHE0006683_16229
13
Corn response
1.00E−102
85
12060384
dbj|BAB20579.1|response






regulator 4



regulator 4 [Zea mays]


87
385
PHE0006884_16703
7
Corn granule-bound
0
89
33321047
gb|AAQ06291.1|granule-






starch synthase



bound starch synthase






precursor



precursor [Zea mays]


88
386
PHE0006691_16237
3

E. Coli NAD(P)H-

0
100
15804152
ref|NP_290191.1|NAD(P)H-






dependent glycerol-



dependent glycerol-3-






3-phosphate



phosphate dehydrogenase






dehydrogenase



[Escherichia coli O157:H7










EDL933]


89
387
PHE0006790_16372
9

Arabidopsis

0
95
15219408
ref|NP_175088.1|chlorophyll a






chlorophyll A



oxygenase [Arabidopsis






oxygenase




thaliana]



90
388
PHE0009143_19932
30

E. Coli StpA

2.00E−57
86
83569970
ref|ZP_00921418.1|DNA-










binding protein H-NS [Shigella











dysenteriae 1012]



91
389
PHE0008406_18830
30
Yeast general amino
0
98
14318464
sp|P43548|Yeast general






acid permease



amino acid permease AGP3


92
390
PHE0008279_18708
30
Corn hypothetical
5.00E−25
73
115462095
gb|EAY96551.1|hypothetical






protein



protein OsI_017784 [Oryza











sativa (indica cultivar-group)]



93
391
PHE0009142_19931
30

E. Coli DNA-binding

3.00E−45
72
15801465
ref|NP_415753.1|global DNA-






protein H-NS



binding transcriptional dual










regulator H-NS [Escherichia











coli K12]



94
392
PHE0008308_18841
30
Corn hypothetical
5.00E−41
89
125537055
gb|EAY83543.1|hypothetical






protein



protein OsI_037502 [Oryza











sativa (indica cultivar-group)]



95
393
PHE0007639_17991
29

Chlorella optimized

0
99
118546
emb|CAA41636.1|glutamate






beta glutamate



dehydrogenase (NADP+)






dehydrogenase



[Chlorella sorokiniana]


96
394
PHE0007646_18170
13

Arabidopsis kelch

0
94
116831441
gb|ABK28673.1|unknown






repeat-containing F-



[Arabidopsis thaliana]






box family protein


97
395
PHE0007765_18182
13

Arabidopsis

1.00E−130
100
15218872
ref|NP_176178.1|glutathione






glutathione



transferase [Arabidopsis






transferase




thaliana]



98
396
PHE0008167_18465
5

Arabidopsis protein

1.00E−163
95
15233846
ref|NP_194179.1|protein






kinase



kinase/serine/threonine kinase/










protein-tyrosine kinase










[Arabidopsis thaliana]


99
397
PHE0008172_18471
5

Arabidopsis putative

2.00E−46
97
4895214
gb|AAD32800.1|putative






thioredoxin H



thioredoxin H [Arabidopsis











thaliana]



100
398
PHE0008183_18479
5

Arabidopsis

0
98
15239123
ref|NP_201371.1|protein






receptor protein



serine/threonine kinase






kinase



[Arabidopsis thaliana]


101
399
PHE0007585_17875
6

Arabidopsis drought-

2.00E−42
87
469112
emb|CAA55322.1|Di21






induced protein



[Arabidopsis thaliana]






(Di21)


101
399
PHE0007585_23902
30

Arabidopsis drought-

2.00E−42
87
469112
emb|CAA55322.1|Di21






induced protein



[Arabidopsis thaliana]






(Di21)


102
400
PHE0006804_16385
6
Rice small subunit of
1.00E−107
100
125553324
gb|EAY99033.1|hypothetical






U2 snRNP auxiliary



protein OsI_020266 [Oryza






factor




sativa (indica cultivar-group)]



103
401
PHE0006703_15751
15

Lactobacillus ATP-

1.00E−164
92
104773899
ref|YP_618879.1|6-






dependent



phosphofructokinase






phosphofructokinase



[Lactobacillus delbrueckii






with ctp



subsp. bulgaricus ATCC










11842]


104
402
PHE0007410_17653
3
Umbelopsis glycerol-
1.00E−144
42
58264662
ref|XP_569487.1|glycerol-3-






3-phosphate O-



phosphate O-acyltransferase






acyltransferase



[Cryptococcus neoformans










var. neoformans JEC21]


105
403
PHE0006854_16456
2
Corn kernel specific
1.00E−115
71
32330681
gb|AAP79887.1|yabby10






yabby



protein [Zea mays]


106
404
PHE0007721_21293
19
Corn homeobox
1.00E−152
77
123183
gb|AAP76321.1|homeobox






transcription factor



transcription factor KNOTTED1






KNOTTED1



[Zea mays]


107
405
PHE0006563_15990
13
Wheat glutamine-
0
97
53680379
gb|AAU89392.1|glutamine-






dependent



dependent asparagine






asparagine



synthetase [Triticum aestivum]






synthetase


107
405
PHE0006563_16140
9
Wheat glutamine-
0
97
53680379
gb|AAU89392.1|glutamine-






dependent



dependent asparagine






asparagine



synthetase [Triticum aestivum]






synthetase


108
406
PHE0006619_16147
13
Corn Delta 1-
0
84
2081612
dbj|BAA19916.1|deltal-






pyrroline-5-



pyrroline-5-carboxylate






carboxylate



synthetase [Oryza sativa






synthetase



(japonica cultivar-group)]


109
407
PHE0006813_16394
13

Bacillus subtilis

1.00E−67
93
16078221
sp|O31607|Hemoglobin-like






Hemoglobin-like



protein yjbl (Truncated BHb)






protein TrHb



(trBHb) [Bacillus Subtilis]


110
408
PHE0006688_16234
13
Corn ribosomal
1.00E−131
99
11467185
sp|P16037|maize Chloroplast






protein S2



30S ribosomal protein S2


111
409
PHE0006635_16164
13

E. Coli methylglyoxal

9.00E−86
100
15800822
ref|NP_286838.1|methylglyoxal






synthase



synthase [Escherichia coli










O157:H7 EDL933]


112
410
PHE0008340_19155
30
Soy hypothetical
1.00E−178
78
92896427
gb|ABE93200.1|conserved






protein



hypothetical protein [Medicago











truncatula]



113
411
PHE0008416_18838
30
Corn putative
0
58
115480880
ref|NP_001064033.1|Os10g0110600






oligopeptide



[Oryza sativa (japonica






transporter



cultivar-group)]


114
412
PHE0008422_18842
30
Corn unknown
1.00E−121
55
115448163
dbj|BAD08083.1|unknown






protein



protein [Oryza sativa (japonica










cultivar-group)]


115
413
PHE0008599_19166
30

Synechocystis

0
95
16330244
ref|NP_440972.1|glycogen






glycogen operon



operon protein; GlgX






protein



[Synechocystis sp. PCC 6803]


116
414
PHE0008375_18732
30

Bacillus

6.00E−95
99
16077136
sp|P37472|Hypoxanthine-






hypoxanthine-



guanine phosphoribosyl-






guanine



transferase (HGPRTase)






phosphoribosyltransferase



[Bacillus subtilis]


117
415
PHE0006712_16273
27

Arabidopsis

4.00E−92
100
15237778
ref|NP_200700.1|unknown






unknown function



protein [Arabidopsis thaliana]


117
415
PHE0006712_16362
13

Arabidopsis

4.00E−92
100
15237778
ref|NP_200700.1|unknown






unknown function



protein [Arabidopsis thaliana]


117
415
PHE0006712_16363
9

Arabidopsis

4.00E−92
100
15237778
ref|NP_200700.1|unknown






unknown function



protein [Arabidopsis thaliana]


117
415
PHE0006712_16364
6

Arabidopsis

4.00E−92
100
15237778
ref|NP_200700.1|unknown






unknown function



protein [Arabidopsis thaliana]


118
416
PHE0008394_18763
9
Corn altered
0
97
121341
sp|P25462|Glutamine






glutamine



synthetase (Glutamate--






synthetase



ammonia ligase) (GS2) [Zea











mays]



119
417
PHE0008395_18764
9
Corn altered
0
97
121341
sp|P25462|Glutamine






glutamine



synthetase (Glutamate--






synthetase



ammonia ligase) (GS2) [Zea











mays]



120
418
PHE0008444_18846
3
Corn glutamine
0
100
112490284
dbj|BAA03430.1|glutamine






synthetase1



synthetase [Zea mays]


121
419
PHE0006819_16400
13

Synechocystis A-

0
100
16331685
sp|Q55393|Diflavin






type flavoproteins



flavoprotein A1 (SsATF573)










(NADH:oxygen










oxidoreductase)










[Synechocystis sp. PCC 6803]


122
420
PHE0007417_17663
15
Corn EIL1
0
76
125543684
gb|EAY89823.1|hypothetical










protein OsI_011056 [Oryza











sativa (indica cultivar-group)]



123
421
PHE0008443_18845
13
Barley asparagine
0
97
13925886
gb|AAK49456.1|glutamine-






synthetase1



dependent asparagine










synthetase 1 [Hordeum











vulgare subsp. vulgare]



124
422
PHE0006818_16399
13

E. coli

0
100
16130617
ref|AP003277.1|flavorubredoxin






flavorubredoxin



oxidoreductase [Escherichia











coli W3110]



125
423
PHE0006579_16031
13
Corn thiazole
0
97
2501189
sp|Q41738|Thiazole






biosynthetic enzyme



biosynthetic enzyme 1-1,










chloroplast precursor [Zea











mays]



126
424
PHE0006808_16389
13
Rice transcription
0
97
115484281
gb|ABG22372.1|CCT motif






factor APRR9 like



family protein [Oryza sativa










(japonica cultivar-group)]


127
425
PHE0008160_18460
5

Arabidopsis

0
100
15234062
ref|NP_195033.1|pyruvate






pyruvate



decarboxylase [Arabidopsis






decarboxylase




thaliana]



128
426
PHE0007571_17834
24
Soy
0
97
7799808
emb|CAB91078.1|






homoglutathione



homoglutathione synthetase






synthetase



[Glycine max]


129
427
PHE0007586_17877
13

Arabidopsis

1.00E−151
96
15225630
gb|AAD32843.1|putative






Chlorophyll a-b



chlorophyll a/b binding protein






binding protein



[Arabidopsis thaliana]


130
428
PHE0006969_16871
13
Rice hypothetical
4.00E−34
100
115451023
gb|EAZ25749.1|hypothetical






protein



protein OsJ_009232 [Oryza











sativa (japonica cultivar-











group)]


131
429
PHE0009729_21717
13

Arabidopsis bHLH

1.00E−91
89
30698765
gb|AAO64014.1|putative










homeodomain leucine zipper










protein [Arabidopsis thaliana]


132
430
PHE0006424_15520
9
photosystem II
3.00E−37
75
15224531
gb|AAM64951.1|photosystem






reaction center



II reaction center 6.1 KD






6.1 KD protein



protein [Arabidopsis thaliana]


133
431
PHE0006392_15480
9
Corn PsbP
9.00E−99
84
115489108
gb|ABA98969.2|Thylakoid










lumenal 21.5 kDa protein










[Oryza sativa (japonica










cultivar-group)]


134
432
PHE0006589_16093
13

Arabidopsis

3.00E−84
100
42563272
dbj|BAE99426.1|thioredoxin-






thioredoxin-like



like protein [Arabidopsis






protein




thaliana]



135
433
PHE0009258_21487
16

Arabidopsis sodium

0
87
15240448
ref|NP_198067.1|NHX1






proton exchanger1



(NA+/H+ EXCHANGER);










sodium:hydrogen antiporter










[Arabidopsis thaliana]


135
433
PHE0009258_20132
34

Arabidopsis sodium

0
87
15240448
ref|NP_198067.1|NHX1






proton exchanger1



(NA+/H+ EXCHANGER);










sodium:hydrogen antiporter










[Arabidopsis thaliana]


136
434
PHE0006680_16226
13
Corn flavanone 3-
1.00E−161
81
18057095
gb|AAL58118.1|putative






hydroxylase



flavanone 3-hydroxylase










[Oryza sativa (japonica










cultivar-group)]


137
435
PHE0006481_16072
13
Corn Os09g0570300
1.00E−145
81
115480783
ref|NP_001063985.1|Os09g0570300






homolog



[Oryza sativa (japonica










cultivar-group)]


138
436
PHE0006269_8820
23

Synechocystis

0
96
16329708
ref|NP_440436.1|hypothetical






conserved



protein slr2048 [Synechocystis






tetratricopeptide



sp. PCC 6803]






repeat protein


138
436
PHE0006269_8821
9

Synechocystis

0
96
16329708
ref|NP_440436.1|hypothetical






conserved



protein slr2048 [Synechocystis






tetratricopeptide



sp. PCC 6803]






repeat protein


139
437
PHE0006783_16365
11

Brassica napus

0
97
31455393
emb|CAD92450.1|amino acid






amino acid



permease 6 [Brassica napus]






permease 6


140
438
PHE0003151_18392
28
Soy aintegumenta 2
1.00E−141
47
38492172
gb|AAR22388.1|ANT-like










protein [Nicotiana tabacum]


141
439
PHE0008272_18723
30

Arabidopsis heat

0
88
15220611
sp|Q9S7U5|Heat shock






shock factor protein 5



transcription factor 5) (HSTF 5)










[Arabidopsis thaliana]


142
440
PHE0008277_18706
30
Yeast chromatin
0
95
6323354
sp|Q06168|Chromatin






structure remodeling



structure remodeling complex






complex protein



protein SFH1 [Saccharomyces






SFH1




cerevisiae]



143
441
PHE0008109_18411
30
Yeast
0
93
6324728
sp|Q12232|Yeast






uncharacterized



uncharacterized protein






protein YOR154W



YOR154W precursor






precursor


143
441
PHE0008109_18568
13
Yeast
0
93
6324728
sp|Q12232|Yeast






uncharacterized



uncharacterized protein






protein YOR154W



YOR154W precursor






precursor


144
442
PHE0008280_18826
30

Sinorhizobium

0
93
16263847
ref|NP_436639.1|putative






putative trehalose



trehalose synthase protein






synthase



[Sinorhizobium meliloti 1021]


145
443
PHE0002424_15825
13
Corn
1.00E−140
77
90398977
gb|EAY96061.1|hypothetical






NADPH:protochlorophyllide



protein OsI_017294 [Oryza






oxidoreductase A




sativa (indica cultivar-group)]



146
444
PHE0006914_16804
13
Corn 26S protease
0
100
115466876
dbj|BAD35266.1|26S protease






regulatory subunit 7



regulatory subunit 7 [Oryza











sativa (japonica cultivar-











group)]


147
445
PHE0006642_16172
14
Rice ent-kaurene
0
97
115468620
dbj|BAD54598.1|ent-kaurene






oxidase



oxidase [Oryza sativa










(japonica cultivar-group)]


148
446
PHE0007578_17852
29

Arabidopsis

2.00E−71
100
18395285
ref|NP_564202.1|unknown






unknown protein



protein [Arabidopsis thaliana]


149
447
PHE0009926_21079
30

Arabidopsis putative

1.00E−84
100
18397475
gb|AAM62982.1|putative dual-






dual-specificity



specificity protein phosphatase






protein phosphatase



[Arabidopsis thaliana]


150
448
PHE0006206_19159
30

Arabidopsis ubiquitin

0
97
15234306
ref|NP_194517.1|ubiquitin-






ligase SINAT5



protein ligase/zinc ion binding










[Arabidopsis thaliana]


151
449
PHE0008274_18705
30

Arabidopsis putative

1.00E−154
95
15222103
gb|AAD43166.1|Putative






BURP domain



BURP domain containing






containing protein



protein [Arabidopsis thaliana]


152
450
PHE0009510_20526
30
Cucurbita
1.00E−164
89
49425361
gb|AAT66041.1|spermidine






spermidine synthase



synthase [Cucumis sativus]


153
451
PHE0009937_21097
34
Cucurbita
1.00E−164
89
49425361
gb|AAT66041.1|spermidine






spermidine synthase



synthase [Cucumis sativus]


154
452
PHE0009927_21080
30

Brassica putative

4.00E−79
88
30679726
dbj|BAC42108.1|putative dual-






dual-specificity



specificity protein phosphatase






protein phosphatase



[Arabidopsis thaliana]


155
453
PHE0006460_15962
13

Arabidopsis

0
86
18394658
ref|NP_564064.1|unknown






unknown protein



protein [Arabidopsis thaliana]


156
454
PHE0006445_15956
13

Arabidopsis allene

1.00E−142
100
18404656
emb|CAC83762.1|allene oxide






oxide cyclase



cyclase [Arabidopsis thaliana]


157
455
PHE0006987_16893
13

Arabidopsis

1.00E−125
99
15241101
dbj|BAB09281.1|calcineurin B-






calcineurin B-like



like protein 2 [Arabidopsis






protein 2




thaliana]



158
456
PHE0004230_15865
13
Corn E2F3 like
0
72
115446539
dbj|BAD73815.1|putative E2F










homolog [Oryza sativa










(japonica cultivar-group)]


159
457
PHE0006814_16395
13

Arabidopsis 2-on-2

2.00E−99
100
18418064
dbj|BAD42999.1|2-on-2






hemoglobin



hemoglobin (GLB3)










[Arabidopsis thaliana]


160
458
PHE0002773_15881
13
Rice glucose-1-
0
100
115465649
ref|NP_001056424.1|Os05g0580000






phosphate



[Oryza sativa (japonica






adenylyltransferase



cultivar-group)]






large subunit 1


161
459
PHE0006060_15842
13
Corn ubiquitin
0
86
60677681
dbj|BAD90972.1|cytochrome






precursor



P450 [Oryza sativa (japonica










cultivar-group)]


162
460
PHE0006850_16451
2
Corn kernel specific
4.00E−73
74
115454365
dbj|BAF45803.1|YABBY2






yabby



protein [Oryza sativa (japonica










cultivar-group)]


163
461
PHE0008043_18199
2
Corn homeobox-9
0
99
40950648
gb|AAR97952.1|rolled leaf1










[Zea mays]


164
462
PHE0000125_18852
13
Corn receiver
1.00E−171
73
51571875
dbj|BAD38854.1|pseudo-






domain (TOC1-like)



response regulator 1 [Oryza






10




sativa (japonica cultivar-











group)]


165
463
PHE0008269_18720
30

Arabidopsis protein

0
96
15222311
ref|NP_172195.1|MAPKKK13;






kinase



protein kinase/protein serine/










threonine kinase/protein-










tyrosine kinase [Arabidopsis











thaliana]



166
464
PHE0008264_18712
30

Arabidopsis

0
97
42567081
ref|NP_194117.3|ubiquitin-






ubiquitin-protein



protein ligase/zinc ion binding






ligase



[Arabidopsis thaliana]


167
465
PHE0002782_20059
8
Corn
0
89
115479643
dbj|BAD17509.1|putative






phosphoglucose



glucose-6-phosphate






isomerase



isomerase [Oryza sativa










(japonica cultivar-group)]


167
465
PHE0002782_22452
13
Corn
0
89
115479643
dbj|BAD17509.1|putative






phosphoglucose



glucose-6-phosphate






isomerase



isomerase [Oryza sativa










(japonica cultivar-group)]


168
466
PHE0009438_20404
9
Corn GNC
8.00E−84
52
115445073
dbj|BAD17612.1|zinc finger










protein-like [Oryza sativa










(japonica cultivar-group)]


169
467
PHE0009429_20392
13
Rice sulphiredoxin
6.00E−68
85
71905635
gb|AAZ52795.1|sulfiredoxin-










like protein [Oryza sativa










(japonica cultivar-group)]


170
468
PHE0009440_20406
13
Yeast sulphiredoxin
3.00E−66
100
6322764
sp|P36077|SRX1_YEAST










Sulfiredoxin


171
469
PHE0008543_18945
13

Agrobacterium

0
97
17937602
ref|NP_534391.1|siroheme






siroheme synthase



synthase [Agrobacterium











tumefaciens str. C58]



171
469
PHE0008543_18946
20

Agrobacterium

0
97
17937602
ref|NP_534391.1|siroheme






siroheme synthase



synthase [Agrobacterium











tumefaciens str. C58]



171
469
PHE0008543_18947
10

Agrobacterium

0
97
17937602
ref|NP_534391.1|siroheme






siroheme synthase



synthase [Agrobacterium











tumefaciens str. C58]



172
470
PHE0006960_16857
8
Rice nitrate
0
96
115448947
dbj|BAD16843.1|putative






reductase



nitrate reductase [Oryza sativa










(japonica cultivar-group)]


173
471
PHE0009427_20389
13

Arabidopsis

1.00E−118
88
30686639
dbj|BAB01781.1|






imidazoleglycerol-



imidazoleglycerol-phosphate






phosphate



dehydratase [Arabidopsis






dehydratase




thaliana]



174
472
PHE0006437_15906
13

Arabidopsis putative

1.00E−170
95
22327062
ref|NP_197926.2|protein






protein kinase



kinase/protein serine/










threonine kinase/protein-










tyrosine kinase [Arabidopsis











thaliana]



175
473
PHE0007409_17652
3
Rice mono- or
0
99
115467862
dbj|BAD33251.1|putative






diacylglycerol



mono- or diacylglycerol






acyltransferase



acyltransferase [Oryza sativa










(japonica cultivar-group)]


176
474
PHE0009426_20387
13
Yeast histidinol
0
100
14318548
dbj|BAA09264.1|histidinol






phosphatase



phosphatase [Saccharomyces











cerevisiae]



177
475
PHE0009430_20393
13
Soy sulphiredoxin
2.00E−45
74
85719364
gb|ABC75369.1|ParB-like










nuclease [Medicago











truncatula]



178
476
PHE0007003_16906
13
Rice putative
1.00E−171
82
115467132
dbj|BAD37240.1|putative






phosphotyrosyl



phosphotyrosyl phosphatase






phosphatase



activator [Oryza sativa






activator



(japonica cultivar-group)]


179
477
PHE0008378_18741
13
Yeast general
1.00E−144
94
6320828
sp|P03069|General control






control protein



protein GCN4 (Amino acid










biosynthesis regulatory










protein) [Saccharomyces











cerevisiae]



180
478
PHE0008273_18725
30

Arabidopsis nucleic

0
95
30683900
ref|NP_180412.2|nucleic acid






acid binding protein



binding [Arabidopsis thaliana]


181
479
PHE0008547_18953
20
Corn ferredoxin
1.00E−38
75
119961
sp|P27789|Maize ferredoxin-5










(Ferredoxin V)


182
480
PHE0006922_16812
13
Corn putative
0
87
115439869
gb|AAZ93623.1|hexokinase 6






hexokinase I



[Oryza sativa (japonica










cultivar-group)]


183
481
PHE0006920_16810
13
Corn putative CAD
0
88
115439215
gb|AAP92128.1|putative






ATPase



ATPase ATP1 [Oryza sativa










(japonica cultivar-group)]


184
482
PHE0008396_18765
9
Corn altered
0
97
121341
sp|P25462|Maize glutamine






glutamine



synthetase (Glutamate--






synthetase2



ammonia ligase) (GS2)


185
483
PHE0006957_16854
13
Corn glutamine
0
97
121341
sp|P25462|Maize glutamine






synthetase2



synthetase (Glutamate--






C299A/C364A



ammonia ligase) (GS2)






double mutant


186
484
PHE0006404_15936
13

Arabidopsis RNA-

0
96
18423760
ref|NP_568826.1|RNA binding






binding protein



protein [Arabidopsis thaliana]


187
485
PHE0006820_16401
13
Nostoc punctiforme
0
97
23129576
ref|ZP_00111402.1|Uncharacterized






A-type flavoproteins



flavoproteins [Nostoc











punctiforme PCC 73102]



188
486
PHE0006690_16236
13
Corn putative high
0
79
108794607
gb|ABG20828.1|high affinity






affinity nitrate



nitrate transporter NRT2.5






transporter



[Hordeum vulgare subsp.











vulgare]



189
487
PHE0006395_15932
13

Arabidopsis leucine-

0
100
18391461
gb|AAW57412.1|plant






rich repeat protein



intracellular Ras-group-related










LRR protein 3 [Arabidopsis











thaliana]



190
488
PHE0006759_16384
6

Arabidopsis glycine

0
100
15235745
ref|NP_195506.1|Serine






hydroxymethyltransferase/



hydroxymethyltransferase 1);






serine



glycine hydroxymethyl-






hydroxymethyltransferase



transferase [Arabidopsis











thaliana]



191
489
PHE0006576_16028
13
Corn tuber-specific
1.00E−87
57
115469098
dbj|BAD37513.1|putative






and sucrose-



tuber-specific and sucrose-






responsive element



responsive element binding






binding factor



factor [Oryza sativa (japonica










cultivar-group)]


192
490
PHE0006979_16885
13

Arabidopsis xylogen

1.00E−103
100
18405294
dbj|BAE73268.1|xylogen like






like protein 12



protein 12 [Arabidopsis











thaliana]



193
491
PHE0009786_21754
9

Arabidopsis HFR1-

1.00E−97
93
18378953
sp|Q9FE22|Long hypocotyl in






delta N105 mutant



far-red 1 (bHLH-like protein










HFR1); gb|AAK15282.1 basic










helix-loop-helix FBI1 protein










[Arabidopsis thaliana]


193
491
PHE0009786_20911
30

Arabidopsis HFR1-

1.00E−97
93
18378953
sp|Q9FE22|Long hypocotyl in






delta N105 mutant



far-red 1 (bHLH-like protein










HFR1); gb|AAK15282.1 basic










helix-loop-helix FBI1 protein










[Arabidopsis thaliana]


194
492
PHE0006572_16007
13

Arabidopsis

1.00E−160
100
18396914
ref|NP_566229.1|Phosphoenol






phosphoenolpyruvate



pyruvate carboxylase kinase 2






carboxylase



[Arabidopsis thaliana]






kinase 2


195
493
PHE0006828_16408
13

Arabidopsis de-

0
94
42566387
ref|NP_192756.2|DET1 (De-






etiolated 1



etiolated 1) [Arabidopsis











thaliana]



196
494
PHE0006588_16092
13
Corn electron
9.00E−90
100
30693659
ref|NP_175021.2|electron






transporter/thiol-



transporter/thiol-disulfide






disulfide exchange



exchange intermediate






intermediate



[Arabidopsis thaliana]


197
495
PHE0006985_16891
13
Corn putative
0
75
115450034
dbj|BAD23003.1|putative






nuclear protein NAP



nuclear protein NAP [Oryza











sativa (japonica cultivar-











group)]


198
496
PHE0006875_16688
13
Rice putative
6.00E−61
85
115479799
dbj|BAD33396.1|putative






Photosystem I



photosystem I reaction center






reaction center



subunit V [Oryza sativa






subunit V



(japonica cultivar-group)]


199
497
PHE0006626_16155
13
Corn (1-3, 1-4)-beta-
1.00E−134
72
18984
emb|CAA36801.1|(1-3,1-4)-






glucanase



beta-D-glucanase;










emb|CAB41401.1|lichenase










[Hordeum vulgare subsp.











vulgare]



200
498
PHE0006689_16235
13
Corn amino acid
0
84
115443611
dbj|BAD08181.1|putative






transporter family



amino acid transport protein






protein



[Oryza sativa (japonica










cultivar-group)]


201
499
PHE0006799_16382
9
Rhodopseudomonas
0
97
39934738
ref|NP_947014.1|Mg-






Mg-protoporphyrin



protoporphyrin IX monomethyl






IX monomethylester



ester oxidative cyclase 66 kD






cyclase



subunit [Rhodopseudomonas











palustris CGA009]



202
500
PHE0006959_16856
8
nitrate reductase
0
95
115476820
dbj|BAD09558.1|nitrate










reductase apoenzyme [Oryza











sativa (japonica cultivar-











group)]


203
501
PHE0011615_23861
31

Arabidopsis

0
78
38049268
gb|AAR10436.1|YDA






activated YODA



[Arabidopsis thaliana]


204
502
PHE0006925_16814
13
Corn translocase
2.00E−74
66
115473245
dbj|BAC83874.1|translocase






inner membrane-like



inner membrane-like protein






protein



[Oryza sativa (japonica










cultivar-group)]


205
503
PHE0006713_16274
25

Arabidopsis cell

0
96
15228343
emb|CAB51062.1|cell division






division cycle protein



cycle protein 23 homolog






23-like



[Arabidopsis thaliana]


206
504
PHE0008447_18848
13
Corn light regulated
1.00E−23
63
77023866
gb|ABA61130.1|light-induced






protein



protein 1 [Lolium perenne]


207
505
PHE0006674_16221
13
Rice putative nitrate
0
95
115441781
dbj|BAD82445.1|putative






transporter NRT1-5



nitrate transporter NRT1-5










[Oryza sativa (japonica










cultivar-group)]


208
506
PHE0010613_22398
3
Corn high oil DAG
1.00E−165
80
115450433
gb|ABF93745.1|expressed






kinase-like



protein [Oryza sativa (japonica










cultivar-group)]


208
506
PHE0010613_22404
7
Corn high oil DAG
1.00E−165
80
115450433
gb|ABF93745.1|expressed






kinase-like



protein [Oryza sativa (japonica










cultivar-group)]


209
507
PHE0002720_22558
18
Corn DNA J protein
0
83
115463255
gb|AAV43840.1|putative DnaJ










protein [Oryza sativa (japonica










cultivar-group)]


210
508
PHE0011760_24005
31

Arabidopsis YODA

0
83
15222512
ref|NP_176557.1|YDA










(YODA); protein kinase/










protein serine/threonine










kinase/protein-tyrosine kinase










[Arabidopsis thaliana]


211
509
PHE0010612_22397
3
Corn high oil nucleic
1.00E−165
77
115450431
gb|ABF93744.1|D111/G-patch






acid binding



domain-containing protein










[Oryza sativa (japonica










cultivar-group)]


211
509
PHE0010612_22403
7
Corn high oil nucleic
1.00E−165
77
115450431
gb|ABF93744.1|D111/G-patch






acid binding



domain-containing protein










[Oryza sativa (japonica










cultivar-group)]


212
510
PHE0010615_22400
3
Corn high oil
1.00E−32
28
125581548
gb|EAZ22479.1|hypothetical






unknown protein



protein OsJ_005962 [Oryza











sativa (japonica cultivar-











group)]


213
511
PHE0007411_17654
3
Rice putative
0
97
57899656
dbj|BAD87325.1|putative






tafazzin isoform



tafazzin isoform [Oryza sativa










(japonica cultivar-group)]


214
512
PHE0010617_22402
3
Corn high oil
5.00E−98
69
115447203
dbj|BAD20134.1|






hydrolase-like



isochorismatase hydrolase-like






protein



protein [Oryza sativa (japonica










cultivar-group)]


215
513
PHE0010610_22395
3
Corn high oil RING-
9.00E−27
75
2894379
emb|CAA74911.1|ring finger






finger protein



protein [Hordeum vulgare










subsp. vulgare]


216
514
PHE0006883_16702
7
Corn glucosyl
0
93
136757
sp|P04713|Granule-bound






transferase



starch synthase 1, (GBSS-I)










emb|CAA27574.1|glucosyl










transferase [Zea mays]


217
515
PHE0010636_22408
3
Corn glucose 6-
0
89
2997589
gb|AAC08524.1|glucose-6-






phosphate



phosphate/phosphate-






translocator



translocator precursor [Zea











mays]



217
515
PHE0010636_22410
7
Corn glucose 6-
0
89
2997589
gb|AAC08524.1|glucose-6-






phosphate



phosphate/phosphate-






translocator



translocator precursor [Zea











mays]



218
516
PHE0011082_23038
30
Soy SAG13 like
3.00E−98
66
15239327
gb|AAO22710.1|putative short










chain alcohol dehydrogenase










[Arabidopsis thaliana]


219
517
PHE0009475_20462
15

Arabidopsis

0
100
15235072
ref|NP_194272.1|gibberellin






gibberellin 20-



20-oxidase/gibberellin 3-beta-






oxidase



dioxygenase [Arabidopsis











thaliana]



219
517
PHE0009475_20468
2

Arabidopsis

0
100
15235072
ref|NP_194272.1|gibberellin






gibberellin 20-



20-oxidase/gibberellin 3-beta-






oxidase



dioxygenase [Arabidopsis











thaliana]



219
517
PHE0009475_20899
1

Arabidopsis

0
100
15235072
ref|NP_194272.1|gibberellin






gibberellin 20-



20-oxidase/gibberellin 3-beta-






oxidase



dioxygenase [Arabidopsis











thaliana]



219
517
PHE0009475_21740
any

Arabidopsis

0
100
15235072
ref|NP_194272.1|gibberellin






gibberellin 20-



20-oxidase/gibberellin 3-beta-






oxidase



dioxygenase [Arabidopsis











thaliana]



219
517
PHE0009475_20469
30

Arabidopsis

0
100
15235072
ref|NP_194272.1|gibberellin






gibberellin 20-



20-oxidase/gibberellin 3-beta-






oxidase



dioxygenase [Arabidopsis











thaliana]



220
518
PHE0007415_17661
7
Corn EIL2
0
75
125543684
gb|EAY89823.1|hypothetical










protein OsI_011056 [Oryza











sativa (indica cultivar-group)]



221
519
PHE0006614_16129
22
Soy STN7-LHCII
0
72
15241093
emb|CAB82763.1|(1-4)-beta-






protein kinase



mannan endohydrolase-like










protein [Arabidopsis thaliana]


222
520
PHE0010611_22396
3
Corn high oil flavin
0
77
116317828
gb|EAY92887.1|hypothetical






containing



protein OsI_014120 [Oryza






monooxygenase




sativa (indica cultivar-group)]



223
521
PHE0011719_23946
na

Arabidopsis 1-

0
99
18410774
ref|NP_567052.1|1-






acylglycerol-3-



acylglycerol-3-phosphate O-






phosphate O-



acyltransferase [Arabidopsis






acyltransferase




thaliana]



224
522
PHE0006861_16463
29

Arabidopsis

0
87
5734789
gb|AAD50054.1|Hypothetical






unknown protein



protein [Arabidopsis thaliana]


225
523
PHE0006568_16005
13
Corn receptor-like
0
60
115472561
dbj|BAC84489.1|putative






protein kinase



serine/threonine-specific










protein kinase [Oryza sativa










(japonica cultivar-group)]


226
524
PHE0010652_22429
3
Corn high oil tRNA
1.00E−172
94
115469828
dbj|BAD53799.1|putative FtsJ






methyltransferase



homolog 1 isoform b [Oryza











sativa (japonica cultivar-











group)]


227
525
PHE0008158_18448
7
ZMEN1
7.00E−65
56
115437056
dbj|BAD73580.1|zinc-binding










protein-like [Oryza sativa










(japonica cultivar-group)]


228
526
PHE0003316_20755
30

E. Coli beta-

0
100
475169
emb|CAA83649.1|beta-






glucuronidase with



glucuronidase [synthetic






intron 2 from potato



construct]






light-inducible






tissue-specific gene


229
527
PHE0009478_20470
2
Corn gibberellin 20
1.00E−167
82
37359180
gb|AAN73384.1|putative






oxidase



gibberellin 20 oxidase [Oryza











rufipogon]



229
527
PHE0009478_20900
1
Corn gibberellin 20
1.00E−167
82
37359180
gb|AAN73384.1|putative






oxidase



gibberellin 20 oxidase [Oryza











rufipogon]



229
527
PHE0009478_20471
any
Corn gibberellin 20
1.00E−167
82
37359180
gb|AAN73384.1|putative






oxidase



gibberellin 20 oxidase [Oryza











rufipogon]



230
528
PHE0010395_21760
30
Soy DUF716
3.00E−84
55
124365521
gb|ABN09755.1|Proteinase










inhibitor 14, serpin [Medicago











truncatula]



231
529
PHE0010391_21753
30
Soy Unknown
7.00E−64
91
115463333
gb|AAV43818.1|unknown






protein



protein [Oryza sativa (japonica










cultivar-group)]


232
530
PHE0010396_21761
30
Soy DUF716
2.00E−83
56
124365521
gb|ABN09755.1|Proteinase










inhibitor I4, serpin [Medicago











truncatula]



233
531
PHE0009511_22422
26
Cucurbita S-
1.00E−147
73
21239731
gb|AAM44307.1|S-






adenosylmethionine



adenosylmethionine






decarboxylase



decarboxylase [Citrofortunella











mitis]



234
532
PHE0010614_22399
3
Corn high oil SOUL-
2.00E−94
80
115435220
dbj|BAA96146.1|putative






like protein



heme binding protein 2 [Oryza











sativa (japonica cultivar-











group)]


235
533
PHE0011420_23634
3
Corn unknown
0
91
115464535
ref|NP_001055867.1|Os05g0482600






protein



[Oryza sativa (japonica










cultivar-group)]


236
534
PHE0011454_23667
13
Corn H2B2
3.00E−36
87
122044864
sp|P05621|Wheat histone










H2B.2


237
535
PHE0011443_24001
13
Corn H2B1
2.00E−39
77
1708107
sp|P54348|Maize histone










H2B.5 (H2B)


238
536
PHE0011452_23665
13
Corn H3-2
3.00E−69
99
15232146
sp|P69246|Maize hHistone










H3.2


239
537
PHE0010394_21758
30
Soy Sin3 associated
2.00E−52
82
115443887
dbj|BAD07609.1|putative P18






ploypeptide



[Oryza sativa (japonica










cultivar-group)]


240
538
PHE0010397_21762
30
Soy unknown
7.00E−17
57
82568700
dbj|BAE48663.1|Pm52 [Prunus






protein




mume]



241
539
PHE0010398_21763
30
Soy unknown
1.00E−17
56
82568700
dbj|BAE48663.1|Pm52 [Prunus






protein




mume]



242
540
PHE0010100_21467
20

Klebsiella nitrite

0
90
585562
gb|AAA25099.1|nitrite






reductase



reductase [Klebsiella oxytoca]


243
541
PHE0011503_23734
1
Corn ethylene
0
91
38607373
gb|AAR25566.1|ethylene






receptor etr1 mutant



receptor [Zea mays]


243
541
PHE0011503_23736
15
Corn ethylene
0
91
38607373
gb|AAR25566.1|ethylene






receptor etr1 mutant



receptor [Zea mays]


244
542
PHE0010099_21319
13

Klebsiella nitrate

0
87
18314343
gb|AAA25100.2|nitrate






reductase large



reductase large subunit






subunit



[Klebsiella oxytoca]


244
542
PHE0010099_21461
9

Klebsiella nitrate

0
87
18314343
gb|AAA25100.2|nitrate






reductase large



reductase large subunit






subunit



[Klebsiella oxytoca]


244
542
PHE0010099_21466
20

Klebsiella nitrate

0
87
18314343
gb|AAA25100.2|nitrate






reductase large



reductase large subunit






subunit



[Klebsiella oxytoca]


245
543
PHE0011269_23400
13

Arabidopsis

0
96
42562138
gb|AAF97271.1|meristem L1






HIO2019A



layer homeobox protein like










(ATML1) from Arabidopsis











thaliana gb|U37589 and











contains Transposase










PF|01527, Homeobox










PF|00046, and START










PF|01852 domains.


246
544
PHE0008393_18762
20
Corn roothairless 1
0
95
34733383
gb|AAQ81632.1|roothairless 1










[Zea mays]


247
545
PHE0010223_21491
3
Corn sucrose
0
87
49066602
gb|AAT51689.1|sucrose






transport protein 4



transport protein [Zea mays]


248
546
PHE0011613_23858
3
Corn WRI1
1.00E−109
56
85815798
dbj|BAE78578.1|aintegumenta-










like [Oryza sativa (japonica










cultivar-group)]


248
546
PHE0011613_23860
7
Corn WRI1
1.00E−109
56
85815798
dbj|BAE78578.1|aintegumenta-










like protein [Oryza sativa










(japonica cultivar-group)]


249
547
PHE0001582_22064
1
Corn ethylene
0
93
10241927
gb|AAR25568.1|ethylene






receptor ETR1 like



receptor [Zea mays]


250
548
PHE0007444_22314
18
Corn aminopropyl
0
94
74481421
gb|AAW57523.1|spermidine






transferase



synthase [Zea mays]


251
549
PHE0010543_22298
16
Corn en-like protein
4.00E−92
94
115498267
gb|ABI98712.1|terminal flower






FDR2



1 [Zea mays]


252
550
PHE0010543_22323
12
Cen-like protein
3.00E−83
94
115498267
gb|ABI98712.1|terminal flower






FDR2



1 [Zea mays]


253
551
PHE0011081_23033
30

Arabidopsis SUC2

0
88
15239921
ref|NP_199174.1|carbohydrate/










sugar transporter [Arabidopsis











thaliana]



254
552
PHE0011075_23026
30
Wheat P-II
3.00E−62
77
125550734
gb|EAY96443.1|hypothetical










protein OsI_017676 [Oryza











sativa (indica cultivar-group)]



255
553
PHE0010100_21462
9

Klebsiella Nitrite

0
90
585562
sp|Q06458|Nitrite reductase






reductase



[NAD(P)H] large subunit










[Klebsiella oxytoca]


256
554
PHE0009640_21775
30

Arabidopsis

3.00E−53
100
15236188
ref|NP_195203.1|unknown






ME10609



protein [Arabidopsis thaliana]


257
555
PHE0010092_21307
13
Corn translation
1.00E−158
84
115452457
gb|ABF95443.1|Eukaryotic






initiation factor 2



translation initiation factor 2






alpha subunit



alpha subunit [Oryza sativa










(japonica cultivar-group)]


258
556
PHE0004611_24123
13

Arabidopsis putative

0
89
12325237
gb|AAG52567.1|putative






peptide transporter



peptide transporter










[Arabidopsis thaliana]


259
557
PHE0010093_21308
13
Corn translation
1.00E−162
85
115452457
gb|ABF95443.1|Eukaryotic






initiation factor 2



translation initiation factor 2






alpha subunit



alpha subunit [Oryza sativa










(japonica cultivar-group)]


260
558
PHE0008605_23084
13

Arabidopsis putative

4.00E−40
98
15226943
gb|AAM62619.1|putative






histone H2B



histone H2B [Arabidopsis











thaliana]



261
559
PHE0009939_21100
16

Arabidopsis glycine

0
98
15234036
ref|NP_195027.1|glycine






dehydrogenase



dehydrogenase [Arabidopsis











thaliana]



262
560
PHE0009941_21105
8
Corn Moc1-like
1.00E−124
80
125540188
gb|EAY86583.1|hypothetical










protein OsI_007816 [Oryza











sativa (indica cultivar-group)]



263
561
PHE0009951_21135
8
Yeast QSR1-like
1.00E−115
93
6323104
sp|P41805|Yeast 60S










ribosomal protein L10 (L9)










(Ubiquinol-cytochrome C










reductase complex subunit VI-










requiring protein)


264
562
PHE0009943_21127
8

Arabidopsis QSR1-

1.00E−127
100
18408550
gb|AAM64974.1|60S






like



ribosomal protein L10










[Arabidopsis thaliana]


265
563
PHE0009948_21132
8
Soy QSR1-like
1.00E−120
93
92870109
gb|ABE79479.1|Ribosomal










protein L10E [Medicago











truncatula]



266
564
PHE0011084_23040
30
Soy
0
77
92896730
gb|ABE93328.1|Peptidase






SNG1_sinapoylglucose:



S10, serine carboxypeptidase






malate



[Medicago truncatula]






sinapoyltransferase


267
565
PHE0008233_23042
30
Soy Phi-1 protein
1.00E−122
71
3759184
dbj|BAA33810.1|phi-1










[Nicotiana tabacum]


268
566
PHE0010854_22730
13
Soy regulator of G-
1.00E−160
58
22331342
ref|NP_189238.2|Regulator of






protein signaling



G-protein signaling 1










[Arabidopsis thaliana]


269
567
PHE0003797_23051
10
Corn DNA-binding
1.00E−44
63
1061306
emb|CAA56287.1|Dof2 [Zea






protein Dof2




mays]



270
568
PHE0010194_21769
30

Arabidopsis putative

0
94
15235301
emb|CAB80722.1|putative






endo-1,4-beta



endo-1,4-beta glucanase






glucanase



[Arabidopsis thaliana]


271
569
PHE0010197_21429
13
Corn putative DNA
2.00E−25
72
115451027
gb|ABF94221.1|bHLH family






binding protein



protein [Oryza sativa (japonica










cultivar-group)]


271
569
PHE0010197_21454
5
Corn putative DNA
2.00E−25
72
115451027
gb|ABF94221.1|bHLH family






binding protein



protein [Oryza sativa (japonica










cultivar-group)]


271
569
PHE0010197_21455
16
Corn putative DNA
2.00E−25
72
115451027
gb|ABF94221.1|bHLH family






binding protein



protein [Oryza sativa (japonica










cultivar-group)]


271
569
PHE0010197_21456
20
Corn putative DNA
2.00E−25
72
115451027
gb|ABF94221.1|bHLH family






binding protein



protein [Oryza sativa (japonica










cultivar-group)]


271
569
PHE0010197_21770
30
Corn putative DNA
2.00E−25
72
115451027
gb|ABF94221.1|bHLH family






binding protein



protein [Oryza sativa (japonica










cultivar-group)]


272
570
PHE0011665_23899
30

Arabidopsis

1.00E−146
96
15225630
gb|AAM20369.1|putative






chlorophyll a/b



chlorophyll a/b binding protein






binding protein



[Arabidopsis thaliana]


273
571
PHE0012178_24443
3
Corn putative chain
4.00E−99
78
115444353
dbj|BAD28032.1|putative






C, structure of the



chain C, structure of the plant






plant transcriptional



transcriptional regulator Pbf-2






regulator Pbf-2



[Oryza sativa (japonica










cultivar-group)]


274
572
PHE0012170_24424
3

Arabidopsis

7.00E−46
74
30689607
gb|ABC96792.1|ARGOS-like






AGROS-like



[Arabidopsis thaliana]


275
573
PHE0011064_22994
4
Corn LPA1
0
85
115436512
dbj|BAD52962.1|unknown










protein [Oryza sativa (japonica










cultivar-group)]


275
573
PHE0011064_23000
8
Corn LPA1
0
85
115436512
dbj|BAD52962.1|unknown










protein [Oryza sativa (japonica










cultivar-group)]


275
573
PHE0011064_23002
18
Corn LPA1
0
85
115436512
dbj|BAD52962.1|unknown










protein [Oryza sativa (japonica










cultivar-group)]


275
573
PHE0011064_23003
9
Corn LPA1
0
85
115436512
dbj|BAD52962.1|unknown










protein [Oryza sativa (japonica










cultivar-group)]


275
573
PHE0011064_23004
10
Corn LPA1
0
85
115436512
dbj|BAD52962.1|unknown










protein [Oryza sativa (japonica










cultivar-group)]


276
574
PHE0010201_21768
30
Soy DNA-binding
9.00E−30
75
30689711
ref|NP_849712.1|transcription






protein



regulator [Arabidopsis thaliana]


277
575
PHE0010838_22702
30
Soy PIN1
3.00E−32
71
25140423
gb|AAN71616.1|PIN-like










protein [Gossypium hirsutum]


278
576
PHE0010201_21433
13
Soy DNA-binding
1.00E−29
75
30689711
ref|NP_849712.1|transcription






protein



regulator [Arabidopsis thaliana]


279
577
PHE0011446_23659
13
Corn histone H3-1
7.00E−59
87
166384
gb|AAA32655.1|histone H3










(H3-1.1)


280
578
PHE0001424_22077
9
Corn seed storage
1.00E−68
41
57233444
gb|AAW48295.1|pore-forming






protein, 35K isoform



toxin-like protein Hfr-2






AmA1-



[Triticum aestivum]


281
579
PHE0011445_23658
13
Corn putative
3.00E−71
89
115447529
dbj|BAD25356.1|putative






elicitor-responsive



elicitor-responsive gene-3






gene-3



[Oryza sativa (japonica










cultivar-group)]


282
580
PHE0010090_21297
21
Corn receptor
0
79
115451705
gb|ABF94768.1|Receptor






protein kinase



protein kinase CLAVATA1






CLAVATA1



precursor, [Oryza sativa










(japonica cultivar-group)]


283
581
PHE0012180_24445
7
Corn regulatory
0
83
138603
sp|P26307|Maize regulatory






protein viviparous-1



protein viviparous-1


284
582
PHE0011083_23039
30
Soy SAG13 like
8.00E−87
63
15239327
ref|NP_196225.1|oxidoreductase;






homolog



dbj|BAA98195.1|short










chain alcohol dehydrogenase-










like [Arabidopsis thaliana]


285
583
PHE0011085_23041
30
Soy
1.00E−157
62
92896727
gb|ABE93325.1|Peptidase






SNG1_sinapoylglucose:



S10, serine carboxypeptidase






malate



[Medicago truncatula]






sinapoyltransferase


286
584
PHE0012175_24440
3
Corn TSO1_like
0
62
115489476
gb|ABA99828.1|










Tesmin/TSO1-like CXC










domain containing protein










[Oryza sativa (japonica










cultivar-group)]


286
584
PHE0012175_24448
7
Corn TSO1_like
0
62
115489476
gb|ABA99828.1|










Tesmin/TSO1-like CXC










domain containing protein










[Oryza sativa (japonica










cultivar-group)]


287
585
PHE0012177_24442
3
Corn P24_like
1.00E−101
74
119638471
gb|ABL85062.1|expressed










protein [Brachypodium











sylvaticum]



288
586
PHE0011447_23660
13
Corn P0031D02.12
1.00E−71
80
115440763
gb|EAY76322.1|hypothetical






protein



protein OsI_004169 [Oryza











sativa (indica cultivar-group)]



289
587
PHE0010194_21426
13

Arabidopsis putative

0
95
15235301
emb|CAB80722.1|putative






endo-1,4-beta



endo-1,4-beta glucanase






glucanase



[Arabidopsis thaliana]


290
588
PHE0011666_23900
30
Soy SPDS
1.00E−165
87
99083515
gb|ABF66657.1|spermidine










synthase [Ammopiptanthus











mongolicus]



291
589
PHE0011448_24160
13
Corn histone H4-2
2.00E−30
80
115479301
ref|NP_001063244.1|










Os09g0433500 [Oryza sativa










(japonica cultivar-group)]


292
590
PHE0008324_18636
13

Arabidopsis T27l1.4

0
86
15218372
ref|NP_172473.1|unknown






protein



protein [Arabidopsis thaliana]


293
591
PHE0006971_16875
13
Ceres82Rice
1.00E−124
84
125583584
gb|EAZ24515.1|hypothetical






unknown protein



protein OsJ_007998 [Oryza











sativa (japonica cultivar-











group)]


294
592
PHE0009939_21147
20

Arabidopsis glycine

0
98
15234036
ref|NP_195027.1|glycine






dehydrogenase



dehydrogenase [Arabidopsis











thaliana]



295
593
PHE0009953_21137
9
Corn dicarboxylate
0
89
40363459
dbj|BAD06219.1|plastidic 2-






translocator 1



oxoglutarate/malate










transporter [Zea mays]


296
594
PHE0006907_16797
13
Corn
5.00E−94
60
115458524
ref|NP_001052862.1|






DUF150_MON_ZM41251



Os04g0438300 [Oryza sativa










(japonica cultivar-group)]


297
595
PHE0008557_18970
13
Corn
0
89
115455751
dbj|BAA07479.1|root






FAD_binding_6[1]::



ferredoxin-NADP+ reductase






NAD_binding_1[1]



[Oryza sativa (japonica






ferredoxin-NADP



cultivar-group)]






reductase


298
596
PHE0006443_15955
13

Arabidopsis putative

0
98
15226918
gb|AAL32813.1|putative SET-






SET-domain protein



domain protein [Arabidopsis











thaliana]











Selection Methods for Transgenic Plants with Enhanced Agronomic Trait


Within a population of transgenic plants regenerated from plant cells transformed with the recombinant DNA many plants that survive to fertile transgenic plants that produce seeds and progeny plants will not exhibit an enhanced agronomic trait. Selection from the population is necessary to identify one or more transgenic plant cells that can provide plants with the enhanced trait. Transgenic plants having enhanced traits are selected from populations of plants regenerated or derived from plant cells transformed as described herein by evaluating the plants in a variety of assays to detect an enhanced trait, e.g. enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. These assays also may take many forms including, but not limited to, direct screening for the trait in a greenhouse or field trial or by screening for a surrogate trait. Such analyses can be directed to detecting changes in the chemical composition, biomass, physiological properties, morphology of the plant. Changes in chemical compositions such as nutritional composition of grain can be detected by analysis of the seed composition and content of protein, free amino acids, oil, free fatty acids, starch or tocopherols. Changes in biomass characteristics can be made on greenhouse or field grown plants and can include plant height, stem diameter, root and shoot dry weights; and, for corn plants, ear length and diameter. Changes in physiological properties can be identified by evaluating responses to stress conditions, for example assays using imposed stress conditions such as water deficit, nitrogen deficiency, cold growing conditions, pathogen or insect attack or light deficiency, or increased plant density. Changes in morphology can be measured by visual observation of tendency of a transformed plant with an enhanced agronomic trait to also appear to be a normal plant as compared to changes toward bushy, taller, thicker, narrower leaves, striped leaves, knotted trait, chlorosis, albino, anthocyanin production, or altered tassels, ears or roots. Other selection properties include days to pollen shed, days to Bilking, leaf extension rate, chlorophyll content, leaf temperature, stand, seedling vigor, internode length, plant height, leaf number, leaf area, tillering, brace roots, stay green, stalk lodging, root lodging, plant health, barreness/prolificacy, green snap, and pest resistance. In addition, phenotypic characteristics of harvested grain may be evaluated, including number of kernels per row on the ear, number of rows of kernels on the ear, kernel abortion, kernel weight, kernel size, kernel density and physical grain quality. Although the plant cells and methods of this invention can be applied to any plant cell, plant, seed or pollen, e.g. any fruit, vegetable, grass, tree or ornamental plant, the various aspects of the invention are preferably applied to corn, soybean, cotton, canola, alfalfa, wheat and rice plants.


The following examples are included to demonstrate aspects of the invention, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific aspects which are disclosed and still obtain a like or similar results without departing from the spirit and scope of the invention.


Example 1
Plant Expression Constructs

This example illustrates the construction of plasmids for transferring recombinant DNA into plant cells which can be regenerated into transgenic plants of this invention


A. Plant Expression Constructs for Corn Transformation

A base corn transformation vector pMON93039, as set forth in SEQ ID NO: 30521, illustrated in Table 3 and FIG. 2, was fabricated for use in preparing recombinant DNA for Agrobacterium-mediated transformation into corn tissue.












TABLE 3








Coordinates of SEQ


Function
Name
Annotation
ID NO: 30521








Agrobacterium

B-AGRtu.right border
Agro right border sequence,
11364-11720


T-DNA transfer

essential for transfer of T-




DNA.


Gene of interest
E-Os.Act1
upstream promoter region of
 19-775


expression

the rice actin 1 gene


cassette
E-CaMV.35S.2xA1-B3
Duplicated35S A1-B3
 788-1120




domain without TATA box



P-Os.Act1
Promoter region of the rice
1125-1204




actin 1 gene



L-Ta.Lhcb1
5′ untranslated leader of
1210-1270




wheat major chlorophyll a/b




binding protein



I-Os.Act1
First intron and flanking
1287-1766




UTR exon sequences from




the rice actin 1 gene



T-St.Pis4
3′ non-translated region of
1838-2780




the potato proteinase




inhibitor II gene which




functions to direct




polyadenylation of the




mRNA


Plant selectable
P-Os.Act1
Promoter from the rice actin
2830-3670


marker

1 gene


expression
L-Os.Act1
First exon of the rice actin 1
3671-3750


cassette

gene



I-Os.Act1
First intron and flanking
3751-4228




UTR exon sequences from




the rice actin 1 gene



TS-At.ShkG-CTP2
Transit peptide region of
4238-4465





Arabidopsis EPSPS




CR-AGRtu.aroA-CP4.nat
Coding region for bacterial
4466-5833




strain CP4 native aroA gene.



T-AGRtu.nos
A 3′ non-translated region of
5849-6101




the nopaline synthase gene




of Agrobacterium





tumefaciens Ti plasmid





which functions to direct




polyadenylation of the




mRNA.



Agrobacterium

B-AGRtu.left border
Agro left border sequence,
6168-6609


T-DNA transfer

essential for transfer of T-




DNA.


Maintenance in
OR-Ec.oriV-RK2
The vegetative origin of
6696-7092



E. coli


replication from plasmid




RK2.



CR-Ec.rop
Coding region for repressor
8601-8792




of primer from the ColE1




plasmid. Expression of this




gene product interferes with




primer binding at the origin




of replication, keeping




plasmid copy number low.



OR-Ec.ori-ColE1
The minimal origin of
9220-9808




replication from the E. coli




plasmid ColE1.



P-Ec.aadA-SPC/STR
Promoter for Tn7
10339-10380




adenylyltransferase




(AAD(3″))



CR-Ec.aadA-SPC/STR
Coding region for Tn7
10381-11169




adenylyltransferase




(AAD(3″)) conferring




spectinomycin and




streptomycin resistance.



T-Ec.aadA-SPC/STR
3′ UTR from the Tn7
11170-11227




adenylyltransferase




(AAD(3″)) gene of E. coli.









Other base vectors similar to the one described above were also constructed as listed in Table 4. These base corn transformation vectors are also used for wheat and rice transformations. See Table 4 for a summary of base vectors and base vector ID's which are referenced in Table 2. Also see Table 5 for a summary of regulatory elements used in the gene expression cassettes for these base vectors and the SEQ ID NOs for these elements.


Primers for PCR amplification of protein coding nucleotides of recombinant DNA are designed at or near the start and stop codons of the coding sequence, in order to eliminate most of the 5′ and 3′ untranslated regions. Each recombinant DNA coding for a protein identified in Table 2 is amplified by PCR prior to insertion into the insertion site within the gene of interest expression cassette of one of the base vectors as referenced in Table 2.










TABLE 4







Base Vector ID
Corn Base Vector





1
pMON92709


2
pMON92713


3
pMON92715


4
pMON92716


5
pMON92718


6
pMON92719


7
pMON92721


8
pMON92722


9
pMON92724


10
pMON92729


11
pMON93025


12
pMON93026


13
pMON93039


14
pMON93043


15
pMON94781


16
pMON94830


17
pMON99009


18
pMON99048


19
pMON99051


20
pMON99052


21
pMON100406






Soy And Canola


Base Vector ID
Base Vector





22
pMON74537


23
pMON74552


24
pMON74553


25
pMON82053


26
pMON83145


27
pMON92671


28
pMON97027


29
pMON99006


30
pMON100407


31
pMON102815


32
pMON102819





Base Vector ID
Cotton Base Vector





33
pMON95549


34
pMON99053






















TABLE 5







SEQ ID

SEQ ID

SEQ ID


Base vector
Promoter
NO
Leader
NO
Intron
NO







pMON92709
P-Os.GT1
30469
L-Os.GT1
30493
I-Zm.DnaK
30516


pMON92713
P-Zm.P39486
30470
L-Zm.39486
30494
I-Zm.DnaK
30516


pMON92715
P-Hv.Per1
30471
L-Hv.Per1
30495
I-Zm.DnaK
30516


pMON92716
P-Zm.FDA
30472
L-Zm.FDA
30496
I-Zm.DnaK
30516


pMON92718
P-Zm.CLK1
30473
L-Zm.Cik1
30497
I-Zm.Cik1
30517


pMON92719
P-Zm.RAB17
30474
L-Zm.RAB17
30498
I-Zm.DnaK
30516


pMON92721
P-Zm.SzeinC1
30475
L-Zm.SzeinC1
30499
I-Zm.DnaK
30516


pMON92722
P-CaMV.35S-enh
30476
L-CaMV.35S
30500
I-Zm.DnaK
30516


pMON92724
P-Zm.-
30477
L-Zm.PPDK
30501
I-Zm.DnaK
30516



636aldolase-0:1:2 +



P-Zm.PPDK


pMON92729
P-Zm.PPDK
30478
L-Zm.PPDK
30501
I-Zm.DnaK
30516


pMON93025
P-At.SUC2
30479
L-At.SUC2
30502
I-Zm.DnaK
30516


pMON93026
P-Os.H1
30480
L-Os.H1
30503
I-Zm.DnaK
30516


pMON93039
E-Os.Act1 + E-
30481
L-Ta.Lhcb1
30504
I-Os.Act1
30518



CaMV.35S.



2xA1-B3 + P-



Os.Act1


pMON93043
P-Zm.EM
30482
L-Zm.EM
30505
I-Zm.DnaK
30516


pMON94781
P-Zm.Brittle-2
30483
L-Zm.Brittle-2
30506
I-Zm.DnaK
30516


pMON94830
P-Os.Act16
30484
L-Os.Act16
30507
I-Os.Act16
30519


pMON99009
P-CaMV.35S-enh
30476
L-Ph.DnaK
30508
None
/


pMON99048
P-Os.Act1 + E-
30485
L-Ta.Lhcb1
30504
I-Os.Act1
30518



CaMV.35S.2xA1-



B3


pMON99051
P-Zm.Kn1
30486
L-Zm.Kn1
30509
None
/


pMON99052
P-Os.Rcc3
30487
L-Os.Rcc3
30510
I-Zm.DnaK
30516


pMON100406
P-Zm.Kn1
30486
L-Zm.Kn1
30509
I-Zm.DnaK
30516


pMON74537
P-At.RbcS4
30488
L-At.RbcS4
30511
None
/


pMON74552
P-CaMV.35S-enh
30476
None
/
None
/


pMON74553
P-CaMV.35S-enh
30476
None
/
None
/


pMON82053
P-CaMV.35S-enh
30476
None
/
None
/


pMON83145
P-Gm.Hsp20
30489
L-Gm.Hsp20
30512
None
/


pMON92671
P-At.SAMS3
30490
L-At.SAMS3
30513
I-At.SAMS3
30520


pMON97027
P-At.GRP7
30491
L-At.GRP7
30514
None
/


pMON99006
P-CaMV.35S-enh
30476
None
/
None
/


pMON100407
P-CaMV.35S-enh
30476
L-Ph.DnaK
30508
None
/


pMON102815
P-At.Erecta
30492
L-At.Erecta
30515
None
/


pMON102819
P-CaMV.35S-enh
30476
None
/
None
/


pMON95549
P-At.SAMS3
30490
L-At.SAMS3
30513
I-At.SMAS3
30520


pMON99053
P-CaMV.35S-enh
30476
L-Ph.DnaK
30508
None
/










For construct pMON94830, the L-Os.Act16 leader sequence (SEQ ID IN 30507) is interrupted by the I-Os.Act16 intron sequence (SEQ ID NO 30519) between nucleotide positions 3344 and 3861.


B. Plant Expression Constructs for Soy and Canola Transformation

Vectors for use in transformation of soybean and canola were also prepared. Elements of an exemplary common expression vector pMON82053 are shown in Table 6 below and FIG. 3. Another example of elements used in plant expression cassestte for gene of interest such as PEP SEQ ID NOs 366 and 521 were the P-Gm.Sphas1 promoter (SEQ ID NO: 30524) and the L-Gm.Sphas1 leader (SEQ ID NO: 30525) sequences.












TABLE 6








Coordinates of


Function
Name
Annotation
SEQ ID NO: 30522








Agrobacterium T-

B-AGRtu.left border
Agro left border sequence, essential for
6144-6585


DNA transfer

transfer of T-DNA.


Plant selectable
P-At.Act7
Promoter from the Arabidopsis actin 7 gene
6624-7861


marker expression
L-At.Act7
5′UTR of Arabidopsis Act7 gene


cassette
I-At.Act7
Intron from the Arabidopsis actin7 gene



TS-At.ShkG-CTP2
Transit peptide region of Arabidopsis
7864-8091




EPSPS



CR-AGRtu.aroA-
Synthetic CP4 coding region with dicot
8092-9459



CP4.nno_At
preferred codon usage.



T-AGRtu.nos
A 3′ non-translated region of the nopaline
9466-9718




synthase gene of Agrobacterium





tumefaciens Ti plasmid which functions to





direct polyadenylation of the mRNA.


Gene of interest
P-CaMV.35S-enh
Promoter for 35S RNA from CaMV
 1-613


expression cassette

containing a duplication of the −90 to −350




region.



T-Gb.E6-3b
3′ untranslated region from the fiber protein
 688-1002




E6 gene of sea-island cotton.



Agrobacterium T-

B-AGRtu.right
Agro right border sequence, essential for
1033-1389


DNA transfer
border
transfer of T-DNA.


Maintenance in E. coli
OR-Ec.oriV-RK2
The vegetative origin of replication from
5661-6057




plasmid RK2.



CR-Ec.rop
Coding region for repressor of primer from
3961-4152




the ColE1 plasmid. Expression of this gene




product interferes with primer binding at the




origin of replication, keeping plasmid copy




number low.



OR-Ec.ori-ColE1
The minimal origin of replication from the
2945-3533





E. coli plasmid ColE1.




P-Ec.aadA-SPC/STR
Promoter for Tn7 adenylyltransferase
2373-2414




(AAD(3″))



CR-Ec.aadA-
Coding region for Tn7 adenylyltransferase
1584-2372



SPC/STR
(AAD(3″)) conferring spectinomycin and




streptomycin resistance.



T-Ec.aadA-SPC/STR
3′ UTR from the Tn7 adenylyltransferase
1526-1583




(AAD(3″)) gene of E. coli.









Primers for PCR amplification of protein coding nucleotides of recombinant DNA are designed at or near the start and stop codons of the coding sequence, in order to eliminate most of the 5′ and 3′ untranslated regions. Each recombinant DNA coding for a protein identified in Table 2 is amplified by PCR prior to insertion into the insertion site within the gene of interest expression cassette of one of the base vectors as referenced in Table 2.


C. Cotton Transformation Vector

Plasmids for use in transformation of cotton were also prepared. Elements of an exemplary common expression vector pMON99053 are shown in Table 7 below and FIG. 4. Primers for PCR amplification of protein coding nucleotides of recombinant DNA are designed at or near the start and stop codons of the coding sequence, in order to eliminate most of the 5′ and 3′ untranslated regions. Each recombinant DNA coding for a protein identified in Table 2 is amplified by PCR prior to insertion into the insertion site within the gene of interest expression cassette of one of the base vectors as referenced in Table 2.












TABLE 7








Coordinates of





SEQ ID NO:


Function
Name
Annotation
30523








Agrobacterium

B-AGRtu.right border
Agro right border sequence,
 1-357


T-DNA transfer

essential for transfer of T-DNA.


Gene of interest
Exp-CaMV.35S-
Enhanced version of the 35S
 388-1091


expression
enh + Ph.DnaK
RNA promoter from CaMV plus


cassette

the petunia hsp70 5′ untranslated




region



T-Ps.RbcS2-E9
The 3′ non-translated region of
1165-1797




the pea RbcS2 gene which




functions to direct




polyadenylation of the mRNA.


Plant selectable
Exp-CaMV.35S
Promoter and 5′ untranslated
1828-2151


marker

region from the 35S RNA of


expression

CaMV


cassette
CR-Ec.nptII-Tn5
Coding region for neomycin
2185-2979




phosphotransferase gene from




transposon Tn5 which confers




resistance to neomycin and




kanamycin



T-AGRtu.nos
A 3′ non-translated region of the
3011-3263




nopaline synthase gene of





Agrobacterium tumefaciens Ti





plasmid which functions to




direct polyadenylation of the




mRNA.



Agrobacterium

B-AGRtu.left border
Agro left border sequence,
3309-3750


T-DNA transfer

essential for transfer of T-DNA.


Maintenance in
OR-Ec.oriV-RK2
The vegetative origin of
3837-4233



E. coli


replication from plasmid RK2.



CR-Ec.rop
Coding region for repressor of
5742-5933




primer from the ColE1 plasmid.




Expression of this gene product




interferes with primer binding at




the origin of replication, keeping




plasmid copy number low.



OR-Ec.ori-ColE1
The minimal origin of
6361-6949




replication from the E. coli




plasmid ColE1.



P-Ec.aadA-SPC/STR
Promoter for Tn7
7480-7521




adenylyltransferase (AAD(3″))



CR-Ec.aadA-SPC/STR
Coding region for Tn7
7522-8310




adenylyltransferase (AAD(3″))




conferring spectinomycin and




streptomycin resistance.



T-Ec.aadA-SPC/STR
3′ UTR from the Tn7
8311-8368




adenylyltransferase (AAD(3″))




gene of E. coli.









Example 2
Corn Transformation

This example illustrates plant cell transformation methods useful in producing transgenic corn plant cells, plants, seeds and pollen of this invention and the production and identification of transgenic corn plants and seed with an enhanced trait, i.e. enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Plasmid vectors were prepared by cloning DNA identified in Table 2 in the identified base vectors for use in corn transformation of corn plant cells to produce transgenic corn plants and progeny plants, seed and pollen.


For Agrobacterium-mediated transformation of corn embryo cells corn plants of a readily transformable line are grown in the greenhouse and ears are harvested when the embryos are 1.5 to 2.0 mm in length. Ears are surface sterilized by spraying or soaking the ears in 80% ethanol, followed by air drying. Immature embryos are isolated from individual kernels on surface sterilized ears. Prior to inoculation of maize cells, Agrobacterium cells are grown overnight at room temperature. Immature maize embryo cells are inoculated with Agrobacterium shortly after excision, and incubated at room temperature with Agrobacterium for 5-20 minutes. Immature embryo plant cells are then co-cultured with Agrobacterium for 1 to 3 days at 23° C. in the dark. Co-cultured embryos are transferred to selection media and cultured for approximately two weeks to allow embryogenic callus to develop. Embryogenic callus is transferred to culture medium containing 100 mg/L paromomycin and subcultured at about two week intervals. Transformed plant cells are recovered 6 to 8 weeks after initiation of selection.


For Agrobacterium-mediated transformation of maize callus immature embryos are cultured for approximately 8-21 days after excision to allow callus to develop. Callus is then incubated for about 30 minutes at room temperature with the Agrobacterium suspension, followed by removal of the liquid by aspiration. The callus and Agrobacterium are co-cultured without selection for 3-6 days followed by selection on paromomycin for approximately 6 weeks, with biweekly transfers to fresh media. Paromomycin resistant calli are identified about 6-8 weeks after initiation of selection.


For transformation by microprojectile bombardment maize immature embryos are isolated and cultured 3-4 days prior to bombardment. Prior to microprojectile bombardment, a suspension of gold particles is prepared onto which the desired recombinant DNA expression cassettes are precipitated. DNA is introduced into maize cells as described in U.S. Pat. Nos. 5,550,318 and 6,399,861 using the electric discharge particle acceleration gene delivery device. Following microprojectile bombardment, tissue is cultured in the dark at 27° C. Additional transformation methods and materials for making transgenic plants of this invention, for example, various media and recipient target cells, transformation of immature embryos and subsequence regeneration of fertile transgenic plants are disclosed in U.S. Pat. Nos. 6,194,636 and 6,232,526 and U.S. patent application Ser. No. 09/757,089, which are incorporated herein by reference.


To regenerate transgenic corn plants a callus of transgenic plant cells resulting from transformation and selection is placed on media to initiate shoot development into plantlets which are transferred to potting soil for initial growth in a growth chamber at 26° C. followed by a mist bench before transplanting to 5 inch pots where plants are grown to maturity. The regenerated plants are self-fertilized and seed is harvested for use in one or more methods to select seeds, seedlings or progeny second generation transgenic plants (R2 plants) or hybrids, e.g. by selecting transgenic plants exhibiting an enhanced trait as compared to a control plant.


Transgenic corn plant cells are transformed with recombinant DNA from each of the genes identified in Table 2. Progeny transgenic plants and seed of the transformed plant cells are screened for enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil as reported in Example 7.


Example 3
Soybean Transformation

This example illustrates plant transformation useful in producing the transgenic soybean plants of this invention and the production and identification of transgenic seed for transgenic soybean having enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.


For Agrobacterium mediated transformation, soybean seeds are imbided overnight and the meristem explants excised. The explants are placed in a wounding vessel. Soybean explants and induced Agrobacterium cells from a strain containing plasmid DNA with the gene of interest cassette and a plant selectable marker cassette are mixed no later than 14 hours from the time of initiation of seed imbibition, and wounded using sonication. Following wounding, explants are placed in co-culture for 2-5 days at which point they are transferred to selection media for 6-8 weeks to allow selection and growth of transgenic shoots. Resistant shoots are harvested approximately 6-8 weeks and placed into selective rooting media for 2-3 weeks. Shoots producing roots are transferred to the greenhouse and potted in soil. Shoots that remain healthy on selection, but do not produce roots are transferred to non-selective rooting media for an additional two weeks. Roots from any shoots that produce roots off selection are tested for expression of the plant selectable marker before they are transferred to the greenhouse and potted in soil. Additionally, a DNA construct can be transferred into the genome of a soybean cell by particle bombardment and the cell regenerated into a fertile soybean plant as described in U.S. Pat. No. 5,015,580, herein incorporated by reference.


Transgenic soybean plant cells are transformed with recombinant DNA from each of the genes identified in Table 2. Transgenic progeny plants and seed of the transformed plant cells are screened for enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil as reported in Example 7.


Example 4
Cotton Transgenic Plants with Enhanced Agronomic Traits

Cotton transformation is performed as generally described in WO0036911 and in U.S. Pat. No. 5,846,797. Transgenic cotton plants containing each of the recombinant DNA having a sequence of SEQ ID NO: 1 through SEQ ID NO: 298 are obtained by transforming with recombinant DNA from each of the genes identified in Table 1. Progeny transgenic plants are selected from a population of transgenic cotton events under specified growing conditions and are compared with control cotton plants. Control cotton plants are substantially the same cotton genotype but without the recombinant DNA, for example, either a parental cotton plant of the same genotype that was not transformed with the identical recombinant DNA or a negative isoline of the transformed plant. Additionally, a commercial cotton cultivar adapted to the geographical region and cultivation conditions, i.e. cotton variety ST474, cotton variety FM 958, and cotton variety Siokra L-23, are used to compare the relative performance of the transgenic cotton plants containing the recombinant DNA. The specified culture conditions are growing a first set of transgenic and control plants under “wet” conditions, i.e. irrigated in the range of 85 to 100 percent of evapotranspiration to provide leaf water potential of −14 to −18 bars, and growing a second set of transgenic and control plants under “dry” conditions, i.e. irrigated in the range of 40 to 60 percent of evapotranspiration to provide a leaf water potential of −21 to −25 bars. Pest control, such as weed and insect control is applied equally to both wet and dry treatments as needed. Data gathered during the trial includes weather records throughout the growing season including detailed records of rainfall; soil characterization information; any herbicide or insecticide applications; any gross agronomic differences observed such as leaf morphology, branching habit, leaf color, time to flowering, and fruiting pattern; plant height at various points during the trial; stand density; node and fruit number including node above white flower and node above crack boll measurements; and visual wilt scoring. Cotton boll samples are taken and analyzed for lint fraction and fiber quality. The cotton is harvested at the normal harvest timeframe for the trial area. Enhanced water use efficiency is indicated by increased yield, improved relative water content, enhanced leaf water potential, increased biomass, enhanced leaf extension rates, and improved fiber parameters.


The transgenic cotton plants of this invention are identified from among the transgenic cotton plants by agronomic trait screening as having increased yield and enhanced water use efficiency.


Example 5
Canola Transformation

This example illustrates plant transformation useful in producing the transgenic canola plants of this invention and the production and identification of transgenic seed for transgenic canola having enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.


Tissues from in vitro grown canola seedlings are prepared and inoculated with overnight-grown Agrobacterium cells containing plasmid DNA with the gene of interest cassette and a plant selectable marker cassette. Following co-cultivation with Agrobacterium, the infected tissues are allowed to grow on selection to promote growth of transgenic shoots, followed by growth of roots from the transgenic shoots. The selected plantlets are then transferred to the greenhouse and potted in soil. Molecular characterization are performed to confirm the presence of the gene of interest, and its expression in transgenic plants and progenies. Progeny transgenic plants are selected from a population of transgenic canola events under specified growing conditions and are compared with control canola plants. Control canola plants are substantially the same canola genotype but without the recombinant DNA, for example, either a parental canola plant of the same genotype that is not transformed with the identical recombinant DNA or a negative isoline of the transformed plant


Transgenic canola plant cells are transformed with recombinant DNA from each of the genes identified in Table 2. Transgenic progeny plants and seed of the transformed plant cells are screened for enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil as reported in Example 7.


Example 6
Homolog Identification

This example illustrates the identification of homologs of proteins encoded by the DNA identified in Table 2 which is used to provide transgenic seed and plants having enhanced agronomic traits. From the sequence of the homologs, homologous DNA sequence can be identified for preparing additional transgenic seeds and plants of this invention with enhanced agronomic traits.


An “All Protein Database” was constructed of known protein sequences using a proprietary sequence database and the National Center for Biotechnology Information (NCBI) non-redundant amino acid database (nr.aa). For each organism from which a polynucleotide sequence provided herein was obtained, an “Organism Protein Database” was constructed of known protein sequences of the organism; it is a subset of the All Protein Database based on the NCBI taxonomy ID for the organism.


The All Protein Database was queried using amino acid sequences provided herein as SEQ ID NO: 299 through SEQ ID NO: 596 using NCBI “blastp” program with E-value cutoff of 1e-8. Up to 1000 top hits were kept, and separated by organism names. For each organism other than that of the query sequence, a list was kept for hits from the query organism itself with a more significant E-value than the best hit of the organism. The list contains likely duplicated genes of the polynucleotides provided herein, and is referred to as the Core List. Another list was kept for all the hits from each organism, sorted by E-value, and referred to as the Hit List.


The Organism Protein Database was queried using polypeptide sequences provided herein as SEQ ID NO: 299 through SEQ ID NO: 596 using NCBI “blastp” program with E-value cutoff of 1e-4. Up to 1000 top hits were kept. A BLAST searchable database was constructed based on these hits, and is referred to as “SubDB”. SubDB was queried with each sequence in the Hit List using NCBI “blastp” program with E-value cutoff of 1e-8. The hit with the best E-value was compared with the Core List from the corresponding organism. The hit is deemed a likely ortholog if it belongs to the Core List, otherwise it is deemed not a likely ortholog and there is no further search of sequences in the Hit List for the same organism. Homologs from a large number of distinct organisms were identified and are reported by amino acid sequences of SEQ ID NO: 597 through SEQ ID NO: 30468. These relationships of proteins of SEQ ID NO: 299 through 596 and homologs of SEQ ID NO: 597 through 30468 are identified in Table 8. The source organism for each homolog is found in the Sequence Listing.









TABLE 8





PEP SEQ ID NO: homolog SEQ ID NOs



























299:
22505
17330
17332
3832
26661
24604
24601
26617
26553
26585
26591
26640



26582
26471
26635
26572
26576
26556
26609
26423
25650
26551
2373
2405



24690
25446
25450
26422
26473
25340
10489
21242
21238
21241
21279
24723



24725
25494
25690
25164
16529
7978
10037
16615
16621
11055
12923
6955



7874
7948
7839
8664
12144
14757
17096
14796
14828
26829
12171
21124



23048
20000
23191
22333
20933
15625
22212
19851
28192
27896
22998
22992



15629
28137
24133
22151
29931
985
2060
944
28955
28930
28990
29965



15658
21486
21255
20071
19073
19014
18158
18127
18109
15620
2239
2233



16398
14851
11657
12588
11744
14570
13748
13790
12143
12170
12173
12919



15622
15628
15656
15604
16393
16394
16392
12916
11037
22432
8701
24656



19135
19853
7817
18495
2869
8263
29437
29438
22440
22435
21711
22465



21146
17031
19010
21907
27742
22415
5429
1188
5421
18365
23435
12382



12090
12087
10571
18362
23640
15382
987
6445
6417
5413
8888
2538



24892
15423
1089
1127
5417
21701
24630
24633
21702
21705
25589
25558



21697
7438
22989
17339
1478
1506
1479
9199
25171
26307
1472
25424



25418
5394
23942
8884
8887
12914
23541


300:
16906
20559
21806
5055
4905
9056
13559
14510
4167
13364
13441
11165



3862
1913
6572
9353
29885
5672
23667
20433
11405
26215
8142
13983



9317
1831
16167
26355
11049
12454
607
20163
21550
8941
3691
19336



8960
11635
2500
12784
9832
13853
14431
6891
25892
24022
26873
8074



10050
6314
15883
23231
829
4758
4754
28646
4717
20464
9858
7971



12622
20501
4271
12195
9291
19503
21548
4124
10854
26029
12272
1775



25590
22837
22812
22556
10678
16223
25625
21334
8456
22176
14327
14302



17822
17624
16859
16850
17780
17619
17629
16852
10588
10650
1001
29219



15690
20293
25766
12151
21485
14320
17504
15478
15477
15066
15835
30365



30421
2696
20311
3417
19744
18488
3839
8782
17965
3743
3775
17509



17514
7891
6457
12394
12411
4075
4077
17590
21909
28286
3388
16788



15982
7848
29135
20349
19157
14248
29979
17396
17358
17398
17407
17324



17410
17366
17403
17357
17368
17360
19996
19846
11904
23714
2173
22814



22809
20197
29687
3983
24318
21492
20319
26103
28858
6802
20161
3448



4055
12112
7750
12606
13406
12501
6292
9527
13807
3896
12598
5152



5149
5155
368
10357
26816


301:
23796
2040
5965
5087
6920
4213
4210
3372
5323
16802
27336
6010



8202
26922
2187
12208
19871
11517
22010
9197


302:
23710
23757
13694
19592
23650
22298
8204
25706
24745
15742
30254
26637



15339
21922
20145
25220
16474
13767
24177
23168
13704


303:
3118
4379
29540
19057
23492
24494
29662
25823
22886
22040
8636


304:
26286
15828
29381
9463
1447
21905
14853
28599
20781
27729
18418
27603



25716
26765
16175
23282
13862
1876
2531
5158
2533
1911
11707
6224



26059
22774
10047
19527
24245
14187
10461
29433
11856
11359
8254
1796



13315
5545
10252
6191
13314
17203
23729
19203
6192
6228
29464
11849



11846
6202
6193
27557
1797
10397
18417
9442
9470
9471
9441
2568



1789
6233
6227
6222
2751
26484
19634
2753
2526
10548
2523
1794



6151
4931
4935
5392
10581
10578
2167
28003
16045
2532
18805
25644



25642
9834
12912
12907
15640
633
25781
4120
9293
11181
8741
3780



18808
26630
26485
24195
4726
377
14947
2501
18997
20773
19301
16345



18906
7189
15422
18210
21903
25270
24910
2503
10083
10113
10084


305:
18758
19672
29878
23393
24854
10271
27282
5416
23140
27083
2820
18194



12159
9628
23204
13486
8110
12511
29407
1503
1698
28122
17599
5085



4965
5960
13895
14534
22724
18476
18472
18083
6734
4997
23491
17752



26978
29291
24877
24879
27070
7649
1949
21111
19488
21730
23945
21686



27651
27650
25269
26607
2212
12263
12554
13918
14019
13994
24823


306:
4073
22555
4100
27267
26975
4996
15934
19811
15228
25434
15706
9550



6935
23481
1480
1482
22482
23641
21374
10611
11281
1517
1518
20953



27704
18146
5286
3202
20016
12556
8797
12122
11246
15875
7987
28966



24975
15587
1524
1520
1525
22082
16220
12190
17779
13034
6068
14309



1994
8624
24174
25476
7577
15375
9142
6515


307:
16517
13655
16944
13657
7250
7253
12523
12555
12496
12558
12553
12525



12447
12531
12529
11716
12499
12463
12453
11714
12577
12494
11711
21462



12491
12601
12582
12580
21464
21465
21507
21503
16115
29908
3927
21531



11721
12449
12456
12564
12574
12579
12597
4697
27420
29700
887
888



29909
10124
10158
3563
3567
2220
19295
11183
6149
23835
18693
5946



11374
7886
10617
22967
2516
25498
16862
19210
19213
20295
12609
20321



19214
21959
21952
9615
9870
2134
24575
19069
12843
24557
21955
9616



5341
17200
24499
19179
16766
27442
27444
27475
11328
16431
22721
27181



5216
30264
17131
27106
28708
27417
27447
877
873
875
871
8082



6820
25860
22904
13900
14638
15177
21498
16830
27002
26518
10906


308:
21299
5872
17817
22477
17074
5710
14819
10604
17874
1813
10959
10605



15093
26425
26689
15978
7997
20445
27568
22238
24487
14436
8017
11460



11177
23024
7463
17377
12864
16004
7043
10148
5131
17879
2644
4412



30069
7754
23829
27906
18649
18440
12984
10731
1103
9112
16047
10098



7912
16569
16562
6731
17991
27109
737
4745
1628
1918
8843
10143



23669
15513
26808
624
8628
24803
29563
22820
5123
10502
15852
29463



22220
10856
18502
23546
13041
11144
7740
22616
15720
20242
23531
5722



20633
5177
20273
19174
27912
17072
2243
16163
25856
5049
6431
1557



7243
920
10764
22406
29189
5927
25074
30314
11963
2240
3307
17229



1852
25569
23447
28980
12872
29015
25605
11835
16360
5509
30059
20235



4232
777
18344
30237
16693
10540
9369
8759
9835
6007
24894
10066



18130
29536
17856
26868
3903
16811
18196
21620
26548
7365
15998
3435



27303
23365
7429
11162
26510
12980
9574
23826
21579
5242
18786
14691



24298
12247
3422
15077
5617
2735
26140
997
29968
12410
12430
10035



14455
7492
23858
24253
17897
14804
22708
1951
3376
2209
19943
4307



28095
17187
11974
15174
24900
18475
20119
16286
18268
17385
22117
27898



8793
15872
15906
4708
21261
26796
17926
27187
29242
3511
15315
28909



14450
15500
18402
15627
29217
28317
25837
1144
10601
28448
696
19865



19308
28313
24999
21607
2359
23902
7170
26313
18751
16086
24401
23719



26186
20994
6420
27239
24526
30413
5823
15225
19802
25521
16776
22806



5296
9126
19695
20534
28027
27517
28414
17210
6379
15657
5267
30048



12519
10872
7999
3409
15362
18419
9898
24104
13253
13534
12783
16649



21079
7630
26772
22421
1477
28203
24992
8063
2672
9815
13717
30388



28256
22915
19937
8726
10192
5739
848
22943
28147
3316
5705
29741



13175
9919
22404
1054
27138
29191
24754
9604
14403
23630
10191
14456



29106
21213
2964
12239
28568
17977
6904
14167
29917
25066
6760
11818



10338
11895
9584
13160
28158
4901
13340
8682
19451
2848
22433
2436



25490
10991
20029
19992
12490
12785
26327


309:
20952
3236
24334
17181
27703
2623
29914
6200
7062
3530
3554
23659



25523
881
10869
12110
6449
24143
20219
18629
16735
28415
10036
20347



20380
24855
4163
9382
18086
13740
12955
3770
21914
17308
26890
5621



4316
22499
13629
7216
14424
1693
15401
20539
26693
4545
6048
10842



24512
4305
18585
2506
19115
14873
12308
18961
24529
9057
20738
7334



29837
22519
24768
3969
15041
24322
23592
29505
3601
11341
994
29145



11268
22722
19591
4525
2445
29611
3707
4339
23545
23449
26341
20094



26889
7940
9580
26304
16101
5572
635
10465
4954
10916
15869
8855



6601
3351
23384
4094
29250
21718
17876
28965
5077
21429
17490
6375



25086
13446
13636
23483
22483
7618
2598
29424
27960
18996
2440
7224



13322
7785
27104
8134
6761
3390
3119
13435
20599
17077
24289
23988



18684
20468
22443
27060
22953
27019
17163
27831
3678
18733
1346
17225



3681
5554
26543
26495
19458
25397
1456
21781
24859
9246
9688
8767



27583
7360
22732
20947
14430
3682
8790
14166
5271
28857
4601
1785



6220
8856
8812
14515
5548
17409
11560
19742
5005
1435
30386
18204



11765
23599
25429
24305
3499
7990
5931
13351
3632
7908
14951
7389



24053
1667
1034
21846
12920
10712
17253
14508
17438
17283
5056
15270



6588
24518
25812
8928
19389
21797
1648
15618
22165


310:
27320
24987
3155
1338
1958
13881
9392
5380
13563
19750
8872
25913



27110
26243
7854
7289
14448
28326
27162
12993
29870
16402
16956
13426



7595
22122
24090
19304
11864
17988
24435
18484
5785
18625
19889
18382



5708
21735
18955
9538
23623
5991
16978
9399
20682
16184
15467
13163



12299
17857
17272
10117
9126
1908
20035
18868
1615
22344
21292
5575



23780
14761
1271
2801
2661
28391
13388
947
29985
8245
4032
23900



2123


311:
6221
29064
17247
6808
12617
7461
25019
27320
12138
11900
28785
24432



20920
5932
28450
24155
15601
19264
24987
7778
9425
9977
3155
13839



8427
13528
9037
22855
8895
10354
1338
26530
6364
28603
978
30171



1958
13881
17361
1702
18189
20590
23012
27156
27068
13620
5486
27182



9677
22229
9392
15962
3210
8742
24082
990
10488
5380
17512
6124



6055
13719
17369
29156
12186
15574
8776
13563
6889
19750
20360
7363



8872
28972
25913
25286
27110
16970
14626
19913
2952
10499
8629
9397



7854
7289
25142
6264
25242
3113
14448
20014
28326
25736
851
26389



29409
27162
3838
4400
21345
29155
19112
1496
12993
29870
26415
24607



16710
29604
10623
23311
16956
3771
3699
26331
13426
28618
30342
10787



22013
13395
26237
19387
22122
1749
7561
12534
7615
6209
14740
13822



10582
29510
1198
29849
2778
3070
3166
17352
19559
9318
10204
19304



11864
11889
20011
11452
13280
2476
24435
30405
5660
11638
18484
5785



24573
13152
3500
14627
20999
5418
8515
13432
14110
18625
16324
19889



23903
14217
18382
22486
11208
24088
18108
5708
21735
18955
1869
25143



29060
2007
5397
18687
9538
12522
23623
5991
16978
19643
9399
28296



9636
29475
29781
23052
2283
1872
20682
2649
25655
16184
15467
27532



22123
13163
23406
23689
20203
25024
28677
26110
22300
16840
12299
13125



22619
23418
17857
17272
1059
17654
18868
2175
1615
2433
22344
3860



13709
5536
13550
10665
16641
9608
18406
26357
25798
10449
9776
23647



12590
8900
3871
27728
25721
21292
23335
24835
5575
23780
14761
26853



4020
1271
22742
23275
7890
9028
27304
8248
6207
23774
13864
6901



12323
28812
5966
23795
779
8321
18640
15951
7688
14971
10176
28391



13388
1939
18213
23163
9301
25502
7903
22199
947
14605
21289
21454



22941
28142
19272
10044
22584
8166
13412
8832
24883
19366
29985
8245



28021
18519
30379
9864
4032
8721
22066
14766
1086
24339
17531


312:
29436
14850
13477
2138
3208
3901
17105
2948
7829
14379
21505
29152



6268
10681
7413
1697
1731
7698
8049
13604
29275
29284
9724
13742



1912
21193
8715
7643
7828
6865
24632
20340
13530
7677
13192
7650



2984
3158
979
13456
6629
12057
26973
23946
3277
12182
17057
2940



27892
16501
6452
2036
11240
6210
22683
3338
29324
4421
21469
20398



7304
3711
8803
17032
4068
13005
20760
28766
1534
5146
20442
11809



25387
26506
13006
18535
7196
7064
7797
19077
5312
3279
8228
20792



18218
17456
21741
28268
3449
1481
24411
24354
25922
1317
29360
20125



30411
3217
23290
13664
3219
25611
15880
25305
24248
20131
7112
2860



10646
5156
1470
1142
7481
27379
21217
6230
16593
24413
20460
13800



16362
8125
29539
20075
11470
1373
26316
5280
7442
9227
24646
18094



7327
7675
20848
17411
10319
5924
24410
6609
3336
26729
13018
1166



30256
14697
2319
12709
15730
1236
20681
10163
8239
19379
2773
13935



9389
23802
17412
25072
16369
18560
16294
18464
29689
24938
5940
29678



23107
19130
20049
9432
5741
3798
22108
7143
5470
18614
26706
20439



19245
18180
22739
29232
17824
7846
24483
6980
19439
28063
28034
28517



25763
20531
15282
7841
21094
8144
23215
26615
21259
26797
24388
18397



27274
2891
17860
21646
29940
13321
1420
9912
19499
21628
3982
29311



7967
16916
7737
29790
1567
25570
914
5088
16568
8596
6487
4080



17527
3796
28784
3642
28118
11444
3001
19749
15976
20597
22491
23919



18063
1508
15331
27385
17740
20542
13927
23800
29486
7823
7753
11283



24645
19328
16305
3555
7085
4166
1076
9070
13431
15843
29128
22802



16427
27445
7383
15299
10655
29283
13136
6028
12296
11261
17252
8509



7087
29978
13414
26066
14846
9161
1622
29281
29277
28982
28106
1945



1948
3473
19244
22787
14026
7390
9284
1214
14000
26960
8568
8408



26923
25805
7557
17660
28819
27080
1854
2004
20328
27090
21887
8492



18262
16472
13196
19626
10568
1737
14022
24551
29274
5794
12100
22587



16387
29535
4332


313:
17074
1813
10959
10605
26425
20445
24487
8017
11177
23024
17377
4412



23829
27906
18440
12984
1103
9112
10098
7912
16569
6731
17991
27109



15513
5123
29463
18502
23546
11144
7740
15720
23531
20633
5177
20273



19174
17072
25856
6431
1557
7243
920
29189
5927
25074
3307
25569



23447
12872
29015
25605
20235
18344
30237
16693
10540
9369
8759
6007



24894
17856
3903
18196
7365
15998
3435
27303
23365
26510
12980
21579



5242
14691
24298
12247
26140
997
12410
12430
14455
7492
17897
14804



2209
19943
4307
28095
17187
24900
18475
16286
17385
22117
27898
8793



15872
15906
26796
29242
3511
28909
14450
15500
28317
10601
28448
24999



6679
2359
26313
18751
16086
24401
23719
26186
28878
24526
15225
16776



22806
5296
22030
19695
27517
28414
17210
6379
30048
7999
3409
15362



18419
24104
1477
2672
9815
13717
30388
28256
19937
8726
5739
3316



23630
10191
14456
28568
17977
6904
11818
9584
13160
28158
8682
2436



25490
10991
12785


314:
9654
9673
21777
15580
11528
13502
11533
23861
24407
28881
9929
15909



2026
2024
7638
7707
18981
4927
13475
6598
27339
12683
27159
8136



11205
14840
17413
8976
21085
5071
2448
10342
18420
21118
28447
1024



3282
9796
8951
7939
21197
22681
27449
24935
6736
18000
11318
880



17374
13543
10709
4698
5976
1821
30380
4667
8746
1571
1995
19769



9883
27269
14225
28504
20918
24317
18252
28199
29834
21756
6606
12161



16555
27422
20200
16342
10305
17133
15606
7528
12805
18973
5126
23875



7430
19393
20409
26205
14250
20631
18778
5604
18595
7297
16963
15136



15135
19183
12794
27123
20160
27325
8420
18843
3853
8018
7422
10462



30217
1449
15623
24442
29425
30351
28863
5115
4153
9250
4081
7562



19629
4705
17899
21080
7799
23958
28418
11541
4323
24962
4441
5928



19504
22840
23320
16031
10320
15441
12039
6174
25674
29249
4442
20579



19864
6356
22935
25802
2113
10844
20659
1874
28045
20108
13080
11030



26034
20373
5727
27347
24097
13266
28543
2125
1297
3298
21535
26376



19745
20768
10468
29066
26645
29554
20649
11031
2131
2254
4730
22057



16885
8070
10596
14924
21006
28643
27597
3628
10128
24115
24861
12868



4090
22353
2006
4106
679
20010
24600
27798
19820
12528
19062
8510



27025
25014
27688
8284
3995
9618
18099
16934
4933
1952
13825
2931



5249
12748
8512
9718
17730
27686
26250
14669
18570
17231
13642
19946



9136
11816
2140
13857
11842
29791
8461
22782
8554
19208
18717
20751



29893
13799
26993
622
8452
7662
27919
12893
7851
6094
8543
3579



29612
8818
29889
5042
9234
3565
13553
30290
22431
4025
18277
10401



23490
30353
26497
19487
18167
26040
16434
29150
12068
11858
9622
5826



12216
14178
7205
5038
20245
30228
20272
6346
25653
21664
4904
10032



29906
11628
7563
13947
16316
3321
26579
11534


315:
3118
4379
29540
2230
27094
3912
25003
2178
21097
303
11912
6746



5259
23092
8636
22040
13730
19057
8657
837
6085
7368
22651
23492



29662
25823
22109
673
5141
29560
23755
2734
22886
23408
13219


316:
6564
7427
6336
20668
22550
26457
13359
16779
26477
19000
11179
17036



3121
30118
27987
15803
14623
27522
26779
9522
5758
29784
11115
24890



9393


317:
22698
12832
6510
17834
3265
3266
15773
10250
2204
30051
2238
8405



5904
17661
6167
4285
19376
6130
26175
5869
28747
5449
6624
7814



25438
21333
12669
24544
2355
16895
23343
16933
2968
9669
14563
7311



28933
17404
27009
12245
2219
21729
27869
23389
12413
20658
9323
7193



16576
8475
24849
10509


318:
18449
21998
22263
19277
5755
4138
9752
21886
17107
20954
21871
21867



21863
21857
21855
21829
21827
21820
21795
21793
21792
21789
21785
21772



21771
21766
21765
21761
20959
20950
20946
20941
20919
20917
20914
20910



20905
20881
20877
20873
20868
20867
20831
20827
20825
20821
20820
20790



20787
20782
21824
21818
20785
21897
20637
20745
19572
20780
20608
19762



19717
19575
19571
20743
20710
20709
20708
20705
20679
20676
20674
20669



20641
20638
20636
20635
20605
20603
20600
20598
19756
19752
19721
19715



19712
19710
19694
19693
19691
19689
19687
19660
19657
19655
19654
19620



19616
19612
19609
19604
19567
19516
20741
20740
20667
19656
19759
20712



19753
19570
20746
19523
13571
25848
3793
18302
24610
22379
6469
17349



24271
7720
21370
6978
1196
24814
10106
5217
7616
24409
4076
6384



7983
24152
23262
24732
19225
13648
20811
1040
24592
9450
22410
19949



10476
13355
16531
19147
5456
11070
1883
20473
18871
4920
23294
23753



3891
14199
30174
26494
26403
26512
26413
12319
17214
27157
23022
13188



24085
18599
29112
4486
21763
11104
16652
7995
11615
6242
4565
29478



6514
751
29501
16056
18737
12351
23018
18292
4444
4445
3521
4448



17267
4418
11430
11427
8643
15491
13686
6088
25417
24759
11563
24750



24718
24753
13852
30117
15661
26934
28121
10590
26942
1316
1320
20034



15779
15535
30070
14860
28134
1625
2698
23360
24636
11118
11119
10752



22693
18670
22670
22700
22307
18243
29362
22720
11958
24449
1566
12619



10543
5772
22625
24323
5775
22034
6513
14730
2754
30114
17842
26470



10704
17770
30003
2442
8480
22913
19311
759
26087
11626
13701
13695



1133
24587
23737
28983
3745
12333
6905
22007
12137
23735
29010
29002



13707
22819
14237
4567
29009
12336
24589
22912
2145
29901
15075
14268



22966
29012
12338
27965
27967
16836
23001
6894
24717
14175
6896
18284



3719
3597
1208
28387
19098
18135
11462
11147
13685
11605
28136
27271



12387
6927
10747
29654
5321
26112
16485


319:
26025
18678
23145
13256
2599
3183
13467
25819
16238
13308
6705
1683



15932
20103
30009
21685
3348
10360
26570
15434
748
26368
14375
13242



23151
7201
17322
1161
6545
9836
10114
3229
25937
8839
7235
949



17714
739
5568
6916
2999
13445
18676
902
26933
4874
25186
19390



27280
18674
10718
23528
29206
8357
28616
12136
21610
22770
7991
29517



2812
7767
16949
2443
23899
30090
5231
23613
24635
17792
2197


321:
5713
5731
7666
23694
22911
18150
26649
2034
10177
30006
8001
4692



29033
14395
29544
19248
15443
10544
24797
10873
7935
29003
15255
4236



3050
19552
16592
11607
21180
26293
619
16587
5988
9434
13332
14583



23776


322:
28075
20429
25267
20452
5815
20569
12740
24463
12571
21240
28866
16145



7644
10890
4953
14249
1859
26961
28687
16646
13103
19372
1075
11012



28763
13279
16909
18634
14200
14252
15965
3957
14353
22537
27917
13290



24094
3042
24671
16559
8844
17230
26537
11160
4101
19149
14848
16476



19844
21324
25887
5879
25536
7634
22302
3583
5654
13582
8655
16714



18408
25134
25133
2085
9245
2169
25665
7734
7755
7733
7758
1434



1433
29679


323:
26396
29158
4822
18244
12967
17134
11009
16725
8007
2954
8852
27845



2629
18814
10620
2865
8453
2299
15558
6294
2856
10541
15612
23099



28255
23798
22907
19283
14119
20596
6506
2591
2566
19934
27102
12576



19816
15649
20313


324:
4695
7074
23887
24207
15227
11280
12210
8914
8949
17044
27663
17180



23934
23176
23961
23927
27394
27343
28223
12241
26039
25285
25527
6322



8016
9270
29224
24488
28695
29408
14433
24132
8404
3641
14676
22027



4437
2493
7857
29733
5336
25833
17806
10942
20040
8399
946
10186



25139
16681
11108
8605
28765
30462
30451
10230
11253
29561
11973
9044



23566
6539
28864
8447
29005
24017
21967
4180
22319
4209
10735
18450



12851
26694
23680
19205
7685
11739
11644
19428
24159
19804
3474
25828



5463
5645
3079
6583
4887
21096
3694
11173
23896
23179
23966
23964



7714
21631
23889
23182
23185
23183
23935
17359
25599
5662
15746
2535



14447
11777
20276
10765
26312
12608
6708
5902
20403
9884
10697
22878



7423
20522
19966
5193
10888
3855
20278
5961
2105
21958
11736
15766



15662
9897
25980
20518
17889
9174
18487
7192
21168
18308
22258
5063



19649
5527
10969
10930
10821
21447
16001
11131
24448
20463
6646
5955



25911
10041
6833
12380
23924
4084
18274
6410
8977
11799
16364
19324



27752
9060
5578
5505
9725
2564
20300
8874
5198
23929
8495
17165



21496


325:
8611
9653
26340
19789
12546
28386
8352
23722
17235
25214
4644
11567



17882
18815
735
8786
21982
29719
19123
15025
1540
22947
4036
7410



24039
22052
2033
25780
20845
21131
691
9520
9519
9596
15571
10045



17616
3844
20503
6259
6256
10247
1982
23494
23476
20841
17362
28976



7855
24763
28796
26709
28664
16116
16110
16108
16105
16150
20118
11117



15068
10850
16042
15543
22735
3988
25929
8584


326:
5173
5172
5168
5169
27559
25561
19375
19137
18295
18861
19386
2279



17868
23434
16705
19125
27453
8946
13210
24880
24875
18157
18159
15947



14260
14257
14254
15950
15944
10855
11632
28068
19547
1934
24077
830



25657
25654
14069
17785
23074
6277
6628
6651
25105
26050
29300
12395



8739
18551
6717
11421
11419
13511
20310
29056
16028
21964
7335
26976



8151
4386
27197
12982
20558
14902


327:
6523
2683
29664
3149
14631
26698
13673
25648
9595
7270
4370
4300



5542
5514
12826
22624
12508
27027
27382
28234
28229
28231
19433
26539



3935
20377
28506
21950
18357
27665
18383
28986


328:
19276
1841
14664
18388
5957
14824
14760
7167
1035
18155
11169
16259



26096
13766
7607
22092
22095
29366
9481
17141
22928
7514
22125
21071



23933
7949
9348
21774


329:
6168
16169
9899
16853
16513
7735
21101
28633
22355
25004
21037
27223



2177
17270
22039
8241
21036
18249
14094
15487
24629
13783
23668
30074



25464
8907
14778
4638
29988
23768
20567


330:
7943
16938
1020
19490
10414
28426
23340
13212
5437
23076
27780
9605



2775
15369
5333
6213
5358
6217
17628
17625
18439
16130
16132
6753



5251
21776
6751
2354
25879
23170
3391
22896
17441
24539
26184
17715



15866
16050
4846
11166
8132
24293
8769
25206
28037


331:
24465
21401
11218
19349
9644
15930
1235
8351
23088
10788
5765
5761



5738
26337
7764
5228
23488
11190
11189
29202
12910
7661
27041
5745



14084
14115
29789
28902
28922
27512
9542
7402
10727
6303
29079
716



8930
24123
23841
1025
2086
2205
9419
7665
17363
11564
30184
30212



19808
370
27115
23497


332:
9181
25440
11322
10394
23194
10121
4183
13824
7065
8038
24714
10120



11384
7161
23913
10483
2137
29194
28776
2787
24809
21173
6535
3144



4366
9814
28454
25455
15353
2377
1885
27695
21552
9640
18639
27572



20691
1325
13958
18343
26799
29048
2200
20277


333:
26921
7592
26493
11706
14645
6556
17968
10624
17587
25908
2924
17103



13861
10511
27191
24114
5142
29172
28080
11050
10690
1510
4359
21319



6781
8848
15538
19869
14896
29647
5668
19736
10237
20371
16072
24961



24791
19941
12201
8982
17218
10782
10597
6729
11210
14810
18962
23027



10203
13462
14488
9807
4423
12684
8425
8870
3915
21985
16768
8704



1565
23789
23820
7418
9151
17139
10241
16395
28549
14696
26260
1956



1703
9974
14018
11287
4189
8195
14013
13078
6214
7142
19515
15065



19121
25225
16111
21188
22049
14688
8348
23070
7954
27039
22516
29328



27544
4425
29377
21474
16358
19897
29844
22622
10011
25784
5095
29886



10887
21494
1699
17405
6742
5555
7654
6372
20556
11433
18121
711



28322
5144
26167
22414
16549
16597
15798
17840
25393
26386
13330
18534



8148
28509
25713
12258
16300
6266
13692
9914
17936
11424
2357
1495



24304
21624
17464
20510
27781
27048
20786
14629
25414
4338
23895
14753



18215
6269
5292
20280
16964
15394
12038
24873
17753
29686
6533
2426



1488
9811
15994
28936
8206
13312
20408
22408
29102
26302
1554
20563



2819
22268
7694
26930
2121
28099
11690
1787
19364
26629
15073
5495



4250
3598
15984
6688
6941
27400
25610
20744
21572
5473
26332
11258



25472
20128
27341
8319
778
9788
13491
8473
9785
13060
2305
4534



11432
7240
19025
6134
13537
23825
22183
13347
21546
2263
27192
14999



11669
16530
10070
3837
26130
15679
11385
3071
7367
19028
15996
24811



25231
18760
23302
2797
1180
30019
17130
18486
10099
4640
12422
2256



28757
11972
10587
6380
15768
2288
28253
5920
24383
26272
6630
30366



28281
2446
20384
8724
627
4476
29103
971
8313
3464
11450
30022



8792
10444
3352
16722
6442
16727
30340
27469
22973
3264
26011
29776



17287
15743
18432
17880
26952
13961
25018
18340
19202
15274
5435
21995



15160
4212
943
29709
23128
8036
17273
18379
30305
28533
6803
3533



26885
23893
6928
28065
24832
21918
2499
23892
18660
6839
28562
12495



25452
23372
951
8972
22734
12787
20346
28751
14496
6087
8910
8697



11602
18658
28451
26909
4399
6778
28644
7020
24981
22402
17087
29329



23420
28028
26733
8106
21509
19461
15474
16610
10594
29838
23279
23544



16578
19700
28770
27715
26058
23037
15867
30376
22462
18967
29967
27622



2882
11130
28361
5759
15942
18029
24677
19079
21622
29035
17723
25854



3944
6911
1013
1285
3866
15522
21141
9022
21237
8516
25349
10774



23023
28634
29555
4833
25567
30236
21630
680
9494
15257
10339
5379



30394
2868
5681
30347
2708
10852
12986
15666
14674
6885
18610
11485



20890
22892
11785
5290
25687
14615
13909
17379
22873
22179
29449
5512



11968
8969
21497
22024
13845
29869
28636
18910
1183
17646
24431
22296



16313
4458
27105
5773
11788
26122
16133
29980
17375
28439
27195
23332



11211
11001
15279
27997
27141
20862
27769
2151
20194
4217
24493
25932



13065
14864
7786
7203
14865
7369
11416
1823
28111
17268
19952
20225



11929
28655
23398
10698
16723
21172
14799
29547
2688
5893
25328
24057



13650
29820
21343
812
1264
1169
23732
14946
10923
7611
13311
8602



10777
20238
23184
29626
23392
7238
29288
3263
21728
18312
12521
7257



26520
5819
2658
23965
18360
14324
15376
11178
9599
13503
22746
17189



26221
2958
11993
15128
16521
22064
17401


334:
17584
24839
25351
25790
5253
27882
27330
1284
24348
7347
21775
6413



3799
16219
22068
15991
22211
25843
19950
28434
29905
17960
29644
26172



9257
3803
12421
1767
27864
19783
18725
23501
21744
1328
25808


335:
1205
1202
8703
21335
24048
24042
18376
12899
18378
20455
6551
19940



19978
19981
12825
2128
18413
18415
16198
3185
15855
2967
2960
2966



4462
28501
25905
11227
28186
984
986
13539
20789
20758
25229
24103



19988
25087
21788
19912
21275
19982
19909
24881
18852
12055
16381
12191



3089
27641
10110
28531
23127
14065
24960
8966
11153
12488
28405
19321



21051
17845
1936
23577
27609
28429
27692
27667
28424
28432
27698
27637



19872
18303
18438
18412
11878
10396
18013
9203
29169
12043
6503
1965



22532
14756
2898
29469
15871
4778
25660
29924
3826
16729
20884
2141



19907
14255
22922
2063
26239
19936
19119
21272
19915
19985
19916
18368



18306
18331
18333
18338
17515
18481
24335
19056
8109
10257
21481


336:
27850
28787
28026
26562
17207
29605
25076
7242
9198
9200
424
17118



13373
22737
1153


337:
3810
8611
17523
26405
19789
12546
11748
24669
1234
10024
16888
15528



17235
25214
4644
8485
29178
11567
18815
24974
12957
27030
735
8786



23475
21423
14874
21982
4004
29719
6724
10758
15025
5033
1540
15117



16450
9585
7687
22955
18744
16796
2152
2153
4039
25006
25631
4196



5802
2614
2033
1607
18080
29921
28076
18341
13401
8330
23494
20841



17362
1450
22986
28262
11117
11357
20471
9486
15543
9266
8762
24787



23218
29190
12248
26945
29898
8006
648
27416
27859


338:
1532
17999
20660
20852
2298
11954
13776
7351
9240
11237
1902
864



7458
11352
5771
28801
14059
8736
8763
25084
15687
2005
5926
12347



7054
29550
23007
7315
24549
14285
16812
10419
11288
14847
13984
3285



6329
18622
1233
16886
3999
18800
7680
6033
17549
8031
8417
15471



21865
7551
5627
8229
7599
16228
23056
23341
1224
26016
2100
24000



7902
3115
9357
6047
8165
11186
14852
20690
19002
14878
14193
2130



24486
24658
29371
16354
13148
13299
4197
23441
8614
29835
6385
8661



11329
2232
30390
13377
4586
30292
26684
4155
3368
27633
9221
25619



29523
9722
8015
11400
20624
17918
27236
25542
29054
8443
8830
20507



20511
9288
7810
12224
23122
25274
11300
16731
18057
1353
28241
19257



21965
24765
21694
1125
7717
6791
22020
1148
14224
22733
24951
21649



6241
9518
13158
14875
24858
5084
22366
7028
12202
24920
17082
9127



11254
30391
20015
26069
24302
29448
25745
5980
26014
26315
14699
2622



18394
11319
6101
27921
13860
3498
1678
3084
17471
29396
3095
14274



17931
22106
23225
9855
11936
13479
19400
7777
12383
17970
3162
4433



22192
8713
12616
17948
28288
24165
15515
18838
21889
7556
23834
7107



11092
1129
17601
9007
9789
12689
14835
3141
25715
2855
29595
29933



15120
26443
11783
11324
11522
12743
25470
16063
15036
3347
19495
16854



18899
24201
12320
22765
6488
5574
26350
7078
26001
13400
2218
14264



7682
30017
9445
12342
12203
4006
29826
29693
30395
10395
4382
27177



23868
12291
4450
15691
12714
17241
23071
18444
14129
22357
15241
17237



19249
15343
29028
21072
6097
23904
4970
1907
6635
4853
21640
5489



1954
17033
8213
22620
10867
7407
25023
12557
15733
27841
29063
8359



11768
8523
10794
7199
27601
11344
15698
15562
18313
25350
7109
15847



16754
24819
8270
29851
17635
5781
25211
4572
602
5629
26726
21733



27410
25749
17944
20520
24876
14501
10115
10755
7998
19299
16232
13097



16748
8979
7952
14568
24480
29273
5197
26225
27768
12870
18503
28013



26560
12219
11508
13068
25354
6146
7354
3044
8777
6995
8302
9965



17333
14357
15939
19984
18700
16989
17416
22345
4461
25893
10943
30285



2135
1165
5209
10901
15915
11482
10546
10549
28528
3250
24130
6957



11591
11622
26697
25532
8580
9770
12812
22831
19560
17766
11391
21678



20871
8932
10629
12754
18823
19085
29701
3430
1588
1283
7080
7660



11436
13176
6315
18768
28840
1822
23115
16121
20995
4376
16306
4524



6782
15389
14317
15764
15155
5082
21232
16190
18907
12297
14962
13933



2791
13699
25215
20587
14328
4661
16129
25195
25196
23200
8201
22384



7619
11403
9694
12362
18675
19398
5522
29356
5552
2465
8000
872



26812
21277
22087
20308
28842
19047
14693
636
13442
27334
24007
23210



25337
11334
29623
15011
8539
12304
29752
12121
28087
12117
8338
29567



1465
12886
28608
1938
22510
964
22511
23166
30028
26098
26125
11479



27876
734
7265
17623
17621
10566
11401
13938
9224
6160
8933
8766



16366
26385
1888
10126
15926
4281
9004
15702
7480
20553
1549
4910



19455
4245
6617
8566
27120
8429
29942
8400
28926
17282
3874
4898


339:
26090
24906
7328
13640
3987
14369
14201
29751
23972
19048
19124
19518



16929
12836
10980
5385
12514
14006
11639
9621
29755
9131
13564
783



18177
14122
16367
18197
16067
10484
27976
27790
10726
28360
7845
26641



12573
11417
1937
8810
11764
27199
26491
7149
27631
850
24218
22409



30220
23301
604
12792
28356
12273
24682
21304
19627
21743
5612
19043



5266
6456
8388
22771
8450
17154
17655
21229
17981
24666
29419
13898



2927
968
25866
11437
11556
29559
28036
22558
28576
18030
20487
13123



6229
21755
4019
14826
8138
13751
26764
8521
15037
12464
15103
18931



1990
16594
2573
20483
23359
14747
4684
21853
9108
9107
22021
22025



24031
21991
26569
29011
3328
15130
15099
21719
26251
13720
2076
26107



29685
2456
2459
2455
27370
27372
13269
13267
451
450
9730
9757



7464
2859
18689
21426
14657
11572
11571
20099
21999
21996
14338
14342



14140
14363
14341
5549
14372
14882
14890
14886
30442
10628
10627
13868



14633
10603
18761
17083
26475
9727
16866
26608
8550
21992
24316
24297



2970
22223
16540
16890
3910
16235
4535
4961
11545
28378
28077
2309



14104
27167
10780
13561
10432
24018
30465
23865
12964
12659
15841
19746



26552
28337
28331
28342
28339


340:
21770
28720
10863
27545
9178
26787
6519
23297
20095
29341
9143
13894



10082
4256
17653
20883
24135
13621
21762
6232
3608
11358
11361
1942



28197
10439
21415
1254
24609
25586
30150
14516
8798
19201
14538
15248



5015
12220
11853
23977
6054
29227
6328
18661
3429
10058
10062
22266



1003
7877
1102
7303
26430
26433
5031
6486
24905
22466
20061
18161



23748
28575
13516
13519
1247
7513
23296
25872
28950
20096
12419
25604



10881
10835
12150
3049
28826
1057
11975
11976
12001
14156
28285
22231



2118
3482
7434
29857
28541
16535
22078


341:
26090
24906
7328
16079
13640
1998
3987
14369
10118
24547
10168
19534



14201
29751
23972
19048
19124
19518
16929
12836
10980
5385
12514
8603



21615
14006
9621
29755
3278
9131
13564
783
18177
14122
16367
18197



10484
27976
27790
10726
28360
7845
8361
7214
3978
10445
26641
12573



11417
1937
8810
18305
17353
26604
11764
14422
27199
13341
7149
27631



850
24218
22409
8455
30360
3313
24294
14091
12152
23301
604
28356



12273
17439
23202
5642
24682
21304
19627
21743
5612
2420
8426
27392



19043
29067
5266
8170
5679
6456
10383
22771
8450
17154
17655
21229



10695
22850
20714
3703
17981
27996
24666
29419
13898
25251
2927
968



25866
11823
11437
11556
29559
28036
22558
28576
18030
644
20487
13123



6229
21755
4019
11073
14826
8138
4868
5476
13751
26764
24215
21989



8521
23457
15037
29764
12464
17472
1453
3416
1990
16594
2573
20483



22565
23359
4684
14747
21853
9107
9108
22028
22025
22021
24031
21991



26569
29011
5830
3328
15099
15130
21719
26251
13720
2076
22740
19986



24231
21220
18601
26295
4537
15313
1807
11247
9328
2456
2455
2459



27366
27372
27370
13267
13269
451
450
9730
9757
9762
588
27732



10917
1804
21426
14657
11572
11571
15508
10874
22276
21996
21999
14338



14342
14140
14363
14341
15131
6669
14372
14890
14882
14886
14908
30442



13868
14633
10627
10628
10603
9996
18761
17083
26475
9727
16415
22604



16765
21893
21349
21992
24297
24316
13140
2970
22223
16540
763
16890



3910
16235
11545
28378
28077
2792
23797
2309
14104
7723
27167
10108



12589
10780
13561
10432
24018
30465
23865
12964
15841
26552
3311


342:
18131
3643
22334
10822
30209
15630
21810
27026
11320
29856
23622
29187



27221
8547
14689
5603


343:
4916
6512
345
2099
26590
29097
7276
21156
11819
9423
11182
10242



5366
29351
25711
2772
18527
8710
4915
11402
11843
12538
3981
12632



13769
9753
20717
23105
611
27849
16602
13617
4590
11101
15611
26384



29416
21142
17846
14279
9447
15785
3589
10641
3749
6668
18679
29123



29078


344:
18538
5825
13713
17120
16141
18556
24151
1677
23605
29721
20155
17125



5796
25356
18349
741
21058
23633
21577
28180
28596
24025
25564
5408



12806
15740
13408
27067
4436
19652
26285
27529
7639
5954


345:
11988
10714
23912
14861
5911
2052
5344
27421
5258
13297
7301
14114



4493
8572
11229
16497
6319
15022
2066
13925
11919
9928
7922
794



17978
6386
8649
4916
6512
11182
10242
29351
29078
343
7164
17921



7227
9612
21317
11922
26878
2772
18527
8710
7789
4915
11402
11843



13769
12538
3981
12632
20717
9753
23105
13617
11101
4590
29416
26384



15611
17846
14279
21142
9447
15785
10641
6668
11800
25436
11027
7276



11819
26590


346:
7938
7964
7962
19565
11649
1841
24052
25942
28102
21218
21222
21226



25813
8989
13495
2802
17982
11467
19113
4583
3706
3705
5387
4585



5393
5465
5445
5501
5472
5471
5448
5444
5469
14414
5998
20172



13331
27590
7516
10486
24909
14569
18186
27256
29588
7905
7870
9487



7775
5987
8194
2774
20986
6870
3660
11002
22398
15468


347:
7938
7964
7962
19565
11649
1841
24052
25942
28102
21218
21222
21226



25813
8989
13495
2802
17982
11467
19113
4583
3706
3705
5387
4585



5393
5465
5445
5501
5472
5471
5448
5444
5469
14414
5998
20172



13331
27590
7516
10486
24909
14569
18186
27256
29588
7905
7870
9487



7775
5987
8194
2774
20986
3779
6870
3660
11002
22398
15468


348:
29418
22857
9311
17119
2104
25847
13088
3752
23347
15078
5810
13222



3310


349:
614
724
3578
22853
3894
14408
17617
16005
5028
4994
6144
18942



1739
19146
19006
21205
19955
18334
18336
688
8318
8309
8009
18573



12586
2590
2565
2467
10589
12733
15544
27685
16097
11960
8314
8418



30026
13264
25960
20279
28604
9168
9102
4334
18164
22788
19095
19662



19042
19049
24125
27121
19723
2837


350:
25920
24644
1529
23814
24344
15084
14227
18006
14646
23448
24200
9536



18059
16068
8120
12166
1157
9942
23891
19467
18975
17571
13916
17062



13954
30032
30072
21873
22881
22429
22033
14744
17913
8446
21245
13213



11202
2023
21284
6453
13606
28247
18363
16827
7499
10306
2885
7034



15748
18795
7135
2826
14349
26534
10017
9581
18077
16809
20911
1848



1845
959
4109
6111
11844
17914
14967
21673
22031
7359
28409
10418



22205
27228
15974
5704
793
8850
1706
29890
23956
11944
17184
1355



27378
12341
6759
28306
28653
18936
29307
17520
27607
5304
17259
26939



26965
29799
2845
2062
2070
15520
12492
15827
1192
30408
1139
15665



19538
9816
13901
7716
19422
15897
12124
21262
15110
25363
7683
28049



27480
18637
24637
26219
19136
7204
22563
15505
24643
5121
4350
4408



3906
23597
8160
8131
2297
2304
25927
7095
17066
29209
25772
29350



29562
5066
3637
28920
3057
6612
7770
20393
27662
28290
24705
16499



19142
30186
3253
4552
27648
23462
16478
16787
28442
22697
23321
3065



10292
26395
22899
19141
6948
12559
19278
5533
27315
9016
7324
4683



6113
15854
18924
20234
22655
26469
17177
14622
22318
755
7895
5074



7569
19265
1098
2571
12840
10817
25634
3112
3358
28513
21346
21320



29139
22925
18545
11825
12196
7530
24976
17397
10164
15770
30252
5196



23259
1359
25426
3667
26115
19548
8687
6627
26061
21214
4028
3534



1345
23886
6923
11242
23901
4165
17152
9868
14083
20806
18263
25213



28694
21031
27059
24261
20030
13360
10494
22152
7226
27863
16168
17204



3394
26821
9744
19873
18837
941
28538
3543
9582
30164
22864
24540



15680
14586
22420
25280
29030
21767
23702
28314
12167
20702
16527
10572



4802
27738
3757
15681
19454
22509
25425
27457
2671
4541
2977
6184



27539
21872
28762
22278
19735
29493
29520
893
25366
16876
6305
25875



8993
28377
2624
2609
4566
21103
21943
17451
1319
29185
21139
28390



28171
6402
9696
14677
27369
26819
27805
22984
14700
11887
8472
14831



26049
7746
20854
7394
3365
3485
27032
3326
26432
23857
9779
26282



30359
22798
10974
10979
18841
11792
4797
2799
30300
5683
21944
30343



23272
23277
10554
22580
11539
11511
9920
2608
9023
14336
22381
14360



28647
10025
28641
28668
2851
7776
22358
20703
18665
697
26328
10907



5004
27589
25190
29584
24187
12314
3043
6989
14709
14830
14402
14008



4431
13366
16410
10783
22185
2470
25841
8458
24608
27170
30062
18922



21399
827
2011
5154
14132
11928
12070
30309
3467
30274
5877
25132



17987
7023
6872
2268
19346
10853
24176
19904
22368
19309
27844
25995



10199
13501
30377
10737
11418
8376
10584
8674
2911
12275
9547
21566



22643
9313
3260
13786
3783
26800
4169
21924
12461
11937
6408
19340



26860
9768
12050
14552
11573
3100
22475
2668
29923
19381


351:
27554
18984
16422
1088
21669
8135
26926
13594
10710
26801
21191
25806



19067
13318
26155
21968
14870
30140
13350
29241
8402
15374
8222
6863



4123
22839
6073
18370
28847
26587
25126
16326
26809
14380
29148
26255



23674
10803
28685
13691
17933
7223
25533
8619
22779
7749
11265
21339



22750
5454
17264
15289
1974
12428
29460
2817
29727
25125
1463
26610



11994
23682
25519
24067
14043
664
4760
13668
15516
4680
9993
5301



8221
863
25180
10815
13635
18926
10248
27340
26253
17205
5052
24731



3006
22102
24591
10529
11102
29117
17395
26634
21754
6987
6542
9467



9217
22531
19097
14241
13334
18834
16236
25886
24311
1160
22903
27329



26308
10311
8833
21593
25617
17687
24914
4346
23671
21206
1309
20939



12229
1669
27028
11980
12133
6357
9205
9991
1562
16106
28483
8149



2711
3792
19632
26474
7977
20623
7684
13424
19673
19421
18576
22127



29532
5384
8604
11038
24779
704
27058
22603
26792
11899
21119
22498



14504
20023
7267
24872
21452
24455
3454
9355
24853
2569
4671
1000



13281
1626
12768
18743
5214
2570
27862
15145
1812
28364
30374
27113



26507
709
20394
3836
11121
7396
3423
3421
795
12040
28488
29630



3405
2732
25092
14244
22841
629
30123
9528
23288
17596
8300
24721



26374
24739
2497
3304
30420
14660
13548
24948
20419
29868
6943
20416



22894
22859
27337
14992
14571
23208
25793
2678
6112
7622
13744
13286



25052
26379
7041
3766
17142
18468
3360
11148
27763
9737
3538
22901



21811
26786
1972
19313
21553
30449
8816
27617
5788
7097
20050
14214



4551
9042
7331
9689
17354
24391
29154
16347
22964
7835
6178
13509



28079


352:
13149
13153
13126
30250
1216
10749
2895
19399
17847
24949
15664
6429



12123
15763
12183
17890
20990
5779
1793
9916
29404
16096
7457
17135



20086
19385
6032
19796
1721
18771
4608
1976
22526
4362
10279
29199



1925
29177
6255
18462
7822
27052
9938
23807
18227
15448
29130
8747


353:
2103
25480
1928
18714
20436
28906
29484
3073
13735
7476
27824
19963



3221
23291
5213
17979
16388
29603
10312
18230
8127
3934
18173
7813



9068
15259
5109
26784
12473
24998
2334
4049


354:
5363
22299
22301
18169
7872
29363
11251
27268
21532
6371
13796
15408



27858
1452
12799
20799
26602
10254
27046
18892
27064
15196
836
29137



2170
20807
16073
6012
4356
2303
2301
2144
7982
7959
3510
3537



3540
16997
11960
20516
1023
21047
18627
13264
25960
20817
19883
9992



13216
21440
18164
22230
2196
10569


355:
8451
16682
1584
27346
18635
8003
19163
12457
7316
6045
6044
17900



22128
8570
23399
30100
25277
12720
6355
26292
16567
1079
8261
25412



9771
29428
23374
22050
2217
1444
29813
17572
13343
28131
29052
27403



23936
20663
16582
7543
29769
19223
789
13517
26690
13362
4302
3466



5327
12303
6407
5508
16712
15042
2939
14381
14524
9050
20655
21318



4880
23357
21527
11747
3488
9924
4579
769
7405
25601
19728
28790



8577
8448
2136
3087
2120
26757
24202
8662
13752
13506
28251
15837



24079
6461
17052
30227
1404
13525
13831
26920
26744
26741
12815
7548



25652
20404
13389
29841
13200
13244


356:
21248
17257
15761
6077
10470
21419
28104
30402
17958
18501
29763
26183



17591
24404


357:
3118
4379
29540
25003
3912
2230
27094
5259
6746
23092
21097
2178



303
11912
22040
13730
19057
8657
6085
7368
837
22651
24494
29662



25823
24934
6049
13521
17683
12327
11243
15290
5880
16826
27443
10119



22886
23408
13219


358:
9617
28607
25085
11495
9739
27311
12711
5185
9235
13544
13808
9009



27602
8237
4455
11195
2458
15832
1788
21739
23423
17693
12584
20756



756
22343
4327
1640
7725
7574
21227
13293


359:
28684
4984
832
5070
2944
20878
5124
1682
28846
11289
28431
1786



12260
12483
6765
22494
24751
7648
6170
19016
15428
12071
3106
23678



4676
2425
23478
15882
4252
25154
22484
16505
16511
6634
22008
16947



17749
14952
11908
6956
6530
10997
12539
10459
10823
19254
29444
22729



22727
22758
22327
1828
22257
29992
27891
22718
19452
19045
1825
1826



15341
15298
15303
15262
15306
15271
15265
15268
15311
15309
15336
25966



28410
14218
18529
22756
6300
11942
22731
18512
29862
27836


360:
28688
17211
26128
17387
23775
5365
26559
10961
18822
10503
22874
29796



14123
7003
21340
11377
5264
4239
18505
2866
20538
21900
7647
9387



18205
26100
7093
15997
12852
18902
8422
22844
30200
17155
5287
19410



26763
20062
7400
6080
9861
16848
25160
18543
15870
18615
23353
19198



16391
28108
9713
24307
17164
23894
6206
3507
1631
2286
28040
30068



12264
13732
29215
24056
23390
30206
28355
29773
21662
28161
2281
26370



14301
23849
30240
12305
29871
15094
3700
10428
17841
9963
29073
16907



16972
8905
3869
29354
27448
27478
9010
12163
7974
5888
7928
10870



15645
17121
16716
25487
24456
2828
3557
14315
20357
14439
22800
18117



1779
7914
1064
9183
20763
16844
4515
15386
9097
29839
13999
18441



13092
18142
8838
9939
26169
20545
25488
10152
2093
15314
8718
21581



2227
26903
20611
19297
20761
15531
9829
17574
5586
14373
10366
29285



25386
30014
27087
22645
17679
18245
25448
3081
28033
24084
5192
6132



9367
764
787
1636
1627
16919
15377
10883
10983
4309
5256
6796



18974
30205
6832
24492
14656
14765
29798
3769
22805
5516
17371
17317



11940
21083
29120
24185
1692
27644
11970
1802
23371
21941
17992
7532



3424
29397
8878
22232
16936
13554
15122
2872
11041
27470
10993
20650



27939
13950
11472
28305
6423
24864
23555
25522
24614
11793
3644
6286



19468
27503
22255
25915
18081
650
12010
20661
27286
27001
29762
8647



9237
10195
22777
9220
18437
18530
2266
12075
7366
28968
10244
14170



21723


361:
20823
2065
3740
29754
27616
16665
3258
29600
23203
5289
8232
9063



13002
29519
5947
19480
13551
12956
13622
16014
10672
2626
1050
19251



29655
20406
9443
10553
29333
6383
1522
23646
16011
30464
18467
27376



24258
15734
21223
811
1519
19496
26946
21837
5538
29836
26932
27468



23870
27575
20557
2174
23926
9038
7620
20397
22248
22851
5602
22100



6652
7388
7409
16957
23824
5434
5440
868
19270
20395
8343
1605



18399
10105
2846
15337
19186
4373
28413
11503
27576
9876
24770
16314



12067
29458
29558
26762
26758
28098
28094
4443
14685
2818
19017
10523



26823
14859
700
9279
22665
7808
16000
965
23839
20428
19429
23065



903
3801
19241
27786
6527
1164
1824
2840
22056
25554
9345
8675



13300
24757
5814
10436
17186
8522
2572
22682
16438
25061
6301
14085



18137
21354
26564
7491
24235


362:
25116
20229
16730
22738
8586
26303
20226
13995
15707
1726
11759
8532



13981
13998
27885
26299
8534
28938
17481
11321
25163
21157
2971
16512



3778
2602
19898
16631
6841
25316
27754
16884
5747
29427
23158
3708



4679
10307
1145
1141
29694
6698
23809
15972
15251
29720
28672
2165



11791
658
11787
25801
6900
8911
25947
3997
7019
6926
28841
24822



29695
21328
10194
13178
29171
4182
1487
2710
16708
11397
2160
25082



19944
12238
11755
28934
12376
1469
28249
19059
23089
22728
23922
23662



23471
26428
1408
13990
26052
28932
13985
963
11250
13791
26321
29119



8525
9308
12348
22463


363:
15667
15776
22048
25867
17058
9020
7889
10022
18296
18319
12699
12695



12694
25226
12728
30056
6650
3103
14968
10608
18148
18528
18524
18552



19492
19418
15886
3386
807
17060
6797
25166
7038
15150
11717
9681



18850
18174
6173
6712
29925
5041
17786
1834
11518
30333
12530
17034



25336
7174
3863
7544
14005
24497
4940
16038
17380
21409
30142
21841



29278
26033
29682
21293
4840
28067
6183
27778
23352
17951
21724
14579



9563
6096
30288
27610
27694
21571
10653
26224
28712
8784
8496
14222



11163
6525
27216
7812
8898
6843
20097
29008
20982
19178
12819
22895



28642
25585
16520
4262
24359
20068
14181
12388
20657
17858
2269
26336



26367
16777
25776
6318
8010
16987
5653
13632
12818
21521
17193
20965



9383
18372
9808
14077
17063
7742
11557
1339
28816
15224
27290
3204



8760
28905
28652
10278
4532
26722
11212
14889
21758
22755
14932
19394



17448
29998
24340
27705
14497
25437
8285
26905
21137
9334
10649
6848



14466
15626
19031
13549
21786
18681
14609
22115
21015
25885
18914
6131



1455
5386
17446
26557
23061
1493
28497
28584
29041
24654
2156
5383



18842
6373
19824
3455
26912
1490
21675
23514
26648
29447
28503
10441



12732
16897
8507
9798
11583
30154
13257
10074
23974
11860
1799
26872



23840
17313
16112
11678
11316
19613
7092
25376
25691
25616
25612
26421



14665
9085
7134
8896
2989
2982
3018
2986
20461
9745
18327
19918



5316
25580
23692
26322
2890
15963
27494
19237
4479
30042
3330
8748



15124
8210
9098
29359
9092
9090
9094
1085
29959
10995
28851
26542



1616
5906
20434
18154
9408
23004
30122
17973
9119
9121
10525
7876



17415
2025
29336
5914
24606
10971
28989
20207
8265
10446
17494
27213



8943
29723
1853
21594
22689
24233
23030
18934
3418
5128
5127
27418



15304
9543
19187
7190
5712
29111
13759
16651
25827
3856
25514
9252



12225
17196
23786
11120
22759
9241
19663
1331


364:
572
22726
21868
23521
25117
27051
5777
25600
16565
22552
25832
16503



21817
14728
10501
20312
1922
26143


365:
2785
19798
21971
22501
8026
7044
7049
7046
18750
13666
3024
3038



11039
14074
1429
8606
24553
1757
17726
17774
14618
22129
28723
16336



19191
22207
23370
9532
17297
9218
2923
24024
18932
4361
17829
15456



2364
6363
12612
6922
19498
13030
8341
28164
4258
13106
1811
16122



1051
744
6529
5135
8908
4810
30047
13016
20550
6474
30079
4836



14390
5894
15952
25952
16984
20565
22999
1037
1906
20526
30247
27474



6834
24461
14477
26589
30015
9849
1281
29262
13270
27735
10808
20109



6263
20296
23246
9911
1792
21541
2813
23854
22660
13873
6244
10328



20192
17484
5593
28954
9444
11167
11388
30348
4972
20561
7018
16985



24560
27578
2880
22305
16973
25664
19344
25467
10696
27992
6966
22703



1278
30261
1231
22393
13639
2521
29348
12061
16655
25510
6733
6732



11771
6758
6444
14367
16745
24525
21445
22666
28946
15182
3933
11820



13952
14634
30178
2943
15060
9260
28357
13601
11022
28236
10389
16217



3746
26696
24173
4154
1411
22141
21833
19331
10144
13294
20437
17754



26947
19779
14906
15100
2107
21251
21025
5624
20001
1696
20726
10463



8705
22313
3107
9503
10905
26121
1864
16828
19196
2247
22768
6517



26490
26065
7392
14197
12658
3963
3966
14009
16539
22561
21947
24506



26284
28532
22833
29368
16464
1131
10026
22163
3111
20784
27389
17201



2905
2904
11350
9206
3509
18221
5579
11463
15132
28420
3182
4791



29620
26713
3292
22543
20897
28545
18113
10753
23273
9930
16623
12945



4291
7077
12747
14095
24406
16041
27952
16991
28566
1376
18300
8551



6522
6532
14836
23677
3289
23409
28366
23851
10686
22521
6218
24927



18916
24313
28124
5915
5547
11080
17068
1558
18371
8407
28877
9854



9856
11911
3309
18232
21621
21105
17867
5002
16760
26441
6986
621



11013
4343
12443
27133
11221
3252
9012
18375
25497
1413
24252
20195



29884
15905
24567
21424
30284
11730
20007
9492
4599
28904
6016
25147



18784
26867
7588
29200
30025
11149
1917
16252
17539
18416
5001
7760



1275
2959
12722
12808
10813
14056
9706
28730
27579


366:
17662
23368
19260
953
2080
23266
7160
11645
1187
23379
25541
3060



30258
23236
15985
23812
18038
15864
23014
24985
23250
23331
7256
29218



6537
8282
6825
16290
25482
5950
2607
10789
10812
1498
1122
26002



16188
19194
21599
29031
11984
20400
13964
4133
25096
30080
24402
6338



7573
18596
30323
16553
29248
30191
3818
16246
13336
15485
9429
7547



2333
25054
20670
27775
8648
5878
21125


367:
12532
1185
15693
12627
19292
28786
14537
28010
20382
18294
18887
18885



5808
17684
768
26489
30213


368:
16906
20559
21806
5055
9056
13559
14510
4167
13364
13441
11165
3862



1913
6572
9353
29885
5672
23667
20433
11405
26215
8142
13983
9317



1831
16167
26355
11049
12454
607
20163
21550
8941
3691
19336
8960



11635
2500
12784
9832
13853
14431
6891
25892
24022
8074
10050
6314



15883
23231
829
4758
4754
28646
4717
20464
9858
7971
12622
20501



4271
12195
9291
19503
21548
4124
10854
6713
26029
12272
1775
25590



22837
22812
22556
10678
16223
25625
21334
8456
22176
14327
14302
17822



17624
16859
16850
17780
17619
17629
16852
10588
10650
1001
29219
15690



20293
25766
12151
21485
14320
17504
15478
15477
15066
15835
30365
30421



2696
20311
3417
19744
18488
3839
8782
17965
3743
3775
17509
17514



7891
6457
12394
12411
4075
4077
17590
21909
28286
3388
16788
15982



7848
29135
20349
19157
14248
29979
17396
17358
17398
17407
17410
17366



17324
17403
17357
17368
17360
19996
19846
11904
23714
2173
22814
22809



20197
29687
3983
24318
21492
20319
26103
28858
6802
20161
3448
4055



12112
10358
7750
12606
13406
12501
6292
9527
13807
3896
12598
5152



5149
5155
26816
300


369:
12105
25662
9969
17226
25561
19375
19137
18295
18861
19386
13836
2279



4873
4876
3820
3091
17868
23434
16705
19125
27453
13210
8946
6913



24880
24875
10855
10851
11632
4175
1889
28068
16417
3807
24970
19547



25083
28222
27062
1397
28093
20514
1934
17351
5340
17475
6651
6628



23848
21516
6717
11419
11421
6570
4635
11278
2641
27197
4386
12120



12982
8875
9649
9303
29572
20558
24896
25979


370:
24465
21401
11218
19349
9644
15930
1235
8351
23088
10788
5765
5761



5738
26337
7764
5228
23488
11190
11189
29202
12910
7661
27041
5745



14084
14115
29789
28902
28922
27512
9542
7402
10727
6303
29079
716



8930
24123
23841
1025
2086
2205
9419
7665
11564
30212
17363
30184



19808
23497
331


371:
20828
2018
29819
22216
5134
18043
29124
3400
30311
10221
10919
3008



15824
27737
18632
30109
912
9609
19414
27547
13910
2325
30046
16563



11184
12474
23560
19933
9705
23325
15597
24532
13595
18630
3813
21147



23666
18452
8173
5530
10225
29269
26847
28198
27549
5423
8517
14755



13305
17340
6951
17444
21075
19610
11191
9314
3484
5919
26028
27327



16925
19302
23459
8937
18668
4087
21301
13146
5106
7387
9531
3669



6992
21099
926
9546
25033
11143
7866
18849
25639
4131
14731
12666



24674
21956
22772
10908
1840
26236
9375
8676
28261
29411
21699
8916



6548
15637
14791
14919
1712
24713
27435
27895
13310
25971
13333
25998



25969
25973
28430
17161
28170
24203
9153
3761
29294
4059
14595
5098



16330
17529
26472
26351
22799
1837
898
10563
10556
16982
23987
28072



3664
25730
13500
8168
18229
24508
7542
27295
25549


372:
29272
16408
7305
25504
30120
18562
6104
429
23539
19011
28591
13463



16590
20339
29410
30183
4993
7120
6985
5112
26266
18260
4678
8552



19030
28248
7679
4918
16318
21717
24060
21344
25297
22138
25346
22012



5650
27496
21794


373:
11083
30232
9501
9499
14909
18574
1318
8956
22144
11445
7700
20469



12900
25243
11836
6695
6670
17570
5294
27600
14930
2838
28257
7119



28769
26445
3685
25444
20074
27152
929
27785
16606
2132
18223
15995



2915
11331
21100
28868
26048
11852
12981
18993
5507
29666
15787
3078



12343
3108
25390
25081
11565
11953
9652
19597
10514
29625


374:
29995
13858
16799
15409
7506
3900
25479
27924
27233
26714
5749
2520



7621
16752
5140
22422
2229
7907
2887
8548
27352
15383
9281
7088



26440
22113
14246
8179
8157
4779
4777
2392
4396
19826
6526
22480



5338
3048
26281
15964
17212
8814
2324
18317
15694
19860
6349
13128



5556
1251
21951
28755
29722
2879
20185
13869
21210
23806
6702
4543



4405
15007
2089
16413
24264
14941
14247
10125
9932
20980
20977
18088



10848
15567
24471
1084
19325
23594
11486
16253
16264
9590
14117
17223



10557
5623
2088
14385
1561
21395
6327
16898
29226
4670
18933
27066



13458
18355
21569
9407
15436
21510
20305
14741
21830
24223
20078
20082



20752
27132
21671
14238
13121
18767
19238
23101
9162
9163
5194
20027



6622
19420
11795
9430
21095
15669
15672
15675
18014
18012
20048
20045



28705
3175
25584
28625
27436
3545
10450
1515
13631
10778
16642
8141



26233
14215
1191
23002
8096
27240
27799
2057
15673
29892
11139


375:
20858
26142
10809
14612
16454
19545
19537
19540
8482
24371
4160
20283



14553
6799
25202
9659
14445
24224
19737
19738
8866
7937
25944
25059



10155
21478
7852
7102
14451
6950
5026
14406
28090
5094
21421
13411



27424
6366
22169
26988
24801
13224
14409
14494
14517
14518
14520
14521



7173
19631
19633
29864
19674
19669
22743
15525
12661
4381
8374
12058



12037
27740
24959
11750
1665
14092
11686
12278
12798
15327
29534
9738



4989
7115
25912
26228
24830
26620
23068
23086
1638
4563
4496
15519



1197
14710
5891
27244
7083
26941
26940
26894
26897
26904
26938
26888



26891
4320
28614
15349
2396
5649
3734
7833
7900
2208
22821
15320



29934
11345
22535
1531
14482
8469
14454
29548
28635
9987
2543
10952



12764
20540
23787
21038
27498
21551
15465
9285
17855
5288
27678
14444



14239
19778
7222
9980
3724
6735
17248
12325
8355
5396
7572
4642



26594
30266
12513
15663
1676
1570
5326
30229
25028
13712
25204
15030



8967
9847
17346
22369
24433
3228
1492
21919
3457
18490
16560
16356



20957
8669
8556
27349
22593
19181
14071
26913
23223
19740
19741
19702



6424
12427
3756
19528
28651
26436
23695
9106
18680
25651
29778
3385



25547
16465
29957
23660
28119
25123
23161
12673
10341
10337
17877
16926



5335
6313
27126
12985
8913
19166
26170
15524
3225
25803
17431
19242



22221
19232
21302
5455
21808
25987
3306
21033
5949
12951
11207
26677



5377
3154
4237
10277
10464
21397
26222
22976
24026
16432
14172
24505



5159
8923
20766
30063
22214
8234
27929
29711
2824
13307
1027
2055



10073
17104
8541
15113
10000
24419
9110
10493
28628
22247
5404
29270



16863
3428
28997
21506
14549
25281
11006
28867
17838
27771
12933
20692



19733
19704
27251
2386
19598
10663
23428
2637
27711
19997
18774
25727



27718
9402
24817
6666
9647
13556
5248
11562
16054
13064
20698
15992



23836
26766
12367
9376
25609
21567
12837
15575
19584
14844
25156
21026



3140
25275
24917
17394
24748
18515
8565
28120
13593
17015
26924
4610



13573
23412
6478
10079
7860
12653
7702
19415
11774
6562
10481
11804



6051
18624
18909
8442
14265
17929
12311
22061
17975
11180
3404
6618



3733
12950
9766
5493
3980
23627
23968
14205
26388
16017
19226
30230



22394
8620
15956
19582
19549
19585
19666
19638
19635
29483
23233
4151



18853
16248
21296
24366
10967
7110
2896
26390
27580
16446
16451
17612



28143
14479
5845
17298
8885
14415
14487
14489
14491
14465
13581
13609



14378
14371
13583
14374
13608
13616
13572
14384
14389
13612
14449
13619



13577
13570
14523
14419
15849
542
21910
14411
16057
19767
23470
27608



15894
16817
25786
11375
29808
2582
28328
20366
22975
16439
22295
21402



19862
28494
14459
1199
24733
4349
14073
5733
23439
29533
4372
13627



8409
28221
27151
25982
25978
25981
26080
25984
26010
25976
26106
26083



25956
25959
26017
26055
26133
26026
26105
26060
26822
26852
26132
26109



26820
26022
26850
26131
26825
26859
26085
26104
26102
26135
26053
26015



26054
26827
26057
26076
26159
20846
26818
26136
26160
26163
20849
20842



20808
20838
26156
20885
21828
20850
20891
26161
20886
25951
20718
26078



25955
19799
20687
19772
21981
19794
19833
19803
21976
21960
19770
21983



20719
19805
20686
20689
26854
19842
16456
21980
19839
19832
21803
19797



26858
16489
20895
20804
21012
20767
20754
20923
20972
20970
16491
21010



20926
20759
21004
20925
20721
16486
21908
21847
21953
20973
20723
21003



20966
20930
20796
21946
21044
21948
21911
21880
21838
21050
20927
21844



20969
21879
21014
21915
21055
21884
21843
21048
21916
21882
21957
20893



21845
21877
2162
1916
5079
2116
14186
4604
4553
4521
13195
24012



20562
18518
6546
13095
2878
25070
5498
20329
27871


376:
9514
18208
27699
24672
695
19688
17787
10946
18811
24157
17433
18211



27587
29573
11124
574
576
16146
13361
26217
825
17239
428
13932



5348


377:
26286
15828
29381
13729
9463
1447
21905
14853
28599
29214
29216
20781



27729
18418
27603
25716
25707
12847
2534
2531
5406
28031
304
13896



16751
5158
2533
10046
13689
22774
1911
1796
5545
10252
13315
6191



13314
17203
23729
2537
6202
11849
11846
6193
27557
1797
10397
9442



9470
2568
9471
9441
1789
6233
19634
2753
6222
6227
2751
26484



2526
2523
10548
1794
6151
4931
4935
5392
10581
10578
28003
18285



19177
10078
18805
25642
25644
9834
9293
12912
12907
633
4120
25781



15640
18808
18210
19601
21903
25270
24910
10083
10084
10113
14947


378:
25474
14771
4723
2695
18286
1459
29180
29184
29182
29179
19650
6427



4021
24424
19821
5833
15452
23315
26642
29704
7761
21612
25030
2327



22167
2330
29528
5148
2361
26318
19158
12389


379:
8336
407
28490
22527
23461
681
14155
24237
27107
21443
2665
27172



24706
20420
5892
8105
6246
22041
16218
457
12930
14021
5230
9685



2316
4924
22112
26146
19293
8274
19356
14025
3477
18809
753
18266



26407
21926
380
25408
9209
8783
19607
25045
5143
16074
18650


380:
8336
28490
6567
22527
14155
27107
27172
5892
6246
16218
457
12930



14021
5230
9685
2316
4924
22112
26146
19293
8274
19356
379
10900



3477
18809
753
18266
26407
19607
25045
21926


381:
16800
8883
21392
15014
12994
30175
24358
9344
16428
17993
26003
11292



4948
4270
7148
7144
11585
29803
7628
23978
14719
15713
26051
23186



21364
21363
12313
19211
12293
3184
21361
4251
29309
11106
8601
3656



16575
10384
2231
6272
4440
25890
14003
23017
26886
10116
11138
3058



29575
12636
14228
16945
13960
28481
24804
18612
23683
1560
30210
18531



30124
14051
2951
28691
12583
14895
20570
29295
29014
15159
12881
19438



17037
23643
18407
22380
13761
21280
30267
2114
20769
10123
3437
6949



16125
22314
4257
7538
22317
29065
671
22201
19766
26705
21568
7030



26970
24582
18078
23130
26658
19377
18835
4249
2051
3595
7527
7053



6212
24385
17648
24821
22780
1333
6654
11266
21602
8339
27388
13866



29872
6882
17417
1384
15154
16018
21174
17763
10140
15398
24041
661



1818
16244
22194
810
28619
11611
9962
3017
19246
28744
7208
12671



672
23171
27081
12060
14764
17741
19417
19866
4641
10040
3665
9316



1443
4097
19724
7171
27471
4065
17292
620
19967
27285
16254
10426



1031
3059
5751
26298
13298
14103
25415
17608
13073
19838
27082
22416



19471
15554
10375
23326
21373
13529
6175
9427
27649
27791
23281
26347



29100
20879
4801
4447
20578
6231
3817
23863
19942
2207
2783
15650



15652
28992
2875
2749
29286
28367
9937
10318
13071
5030
20791
13306



4353
5500
16715
20116
9338
11613
17692
17546
17543
17784
17742
17699



17737
17696
17656
17533
17750
17649
17745
17618
17581
17746
17578
17776



17611
17771
17576
17575
17541
17781
17580
28563
28525
17689
29296
28524



29338
28567
28522
29382
29305
28486
29334
29299
28530
28485
28589
28586



28588
28487
29292
29379
28491
28592
28571
29302
29344
28569
29383
28526



29342
29335
28590
28564
11658
19964
2715
3367
5854
12104
13405
24584



12771
4129
14512
13409
15647
27015
7234
8595
4345
7033
16467
23527



26124
22096
14235
8758
13036
17632
2019
3016
687
30211
23375
4883



27225
1110
27730
19218
25296
15643
4998
13345
11303
28411
17555
10819



9658
20264
9656
16736
17026
13302
27306
27308
26348
16197
12340
21834



15619
5295
5325
5349
5319
8477
29481
2737
2740
2743
25884
28722



12157
18095
21273
982
21276
8514
21387
2038
9428
9426
9406
2039



28482
4030
1407
22661
22664
22626
1244
2512
25499
18163
2228
1267



2486
2513
2472
23931
7782
1424
2482
1430
1393
1241
1427
1273



1431
25534
1323
9583
29887
26073
1270
1390
17236
6763
9490
9404



9433
9458
17758
17797
17793
17680
9436
17721
17762
17716
9469
9464



9460
9466
9498
17799
9495
17710
9489
17764
17705
9055
693
27326



7084
2475
2479
17801
2042
29402
8012
14509
7859
17826
17505
13261



7957
2045
17507
2047
10364
27773
11896
27150
13110
1341
6977
2048



28835
19388
16841
28869
28890
28779
2075
19681
17476
2077
29442
14157



19919
22184
27747
28149
25245
21260
21257
21303
13243
5590
10785
11128



18281
9736
10648
12566
10739
10616
10472
11621
11554
10467
11661
10517



9731
10518
10519
8358
7079
21804
27252
20822
28597
21562
8288
6342



16743
21832
2502
12234
25288
26621
26623
26625
22586
22585
22611
22583



22617
22590
22581
22614
22658
11770
23025
18987
4575
10505
26256
29981


382:
1205
1202
23043
19105
13208
6551
6559
19940
19978
19981
4490
16198



1306
1301
1305
14162
22654
23684
22719
28186
984
986
28534
25229



27701
17690
19988
21788
25087
19912
21275
19982
19909
18852
1894
7697



1981
27932
27807
17275
17258
26144
8753
10153
2234
20381
10110
28531



20355
28470
5936
14511
15482
19230
2975
29138
8877
5395
18703
5364



19321
21051
17845
28539
28536
28429
27692
25933
28424
27698
27667
28432



27637
19792
18013
13358
27690
9342
6730
28714
11798
8785
17920
25660



16729
26290
19907
14255
26258
26234
26261
19936
19119
21272
19915
19985



13259
19916
6608
29643
20900
5842


383:
0


384:
29743
28452
28384
7106
15703
29461
18893
6003
3086
4606
25491
17772



6933
10268
14470
14190
3145
9692
7299
5291
28343
28601
930
15288



26611
1829
7925
17355
15633
7031
28578
30033
4956
8779
16006
17318



2748
18821
21598
27933
16171
6850
25460
12668
24351
24345
24349
14361



24867
16572
22348
21002
29297
9784
10736
26297
27736
30295
23749
16124


385:
25583
25602
25606
25629
25635
25666
25668
25671
25675
25704
25708
30450



19353
2616
2612
11581
2606
3607
2652
2656
4666
4562
4595
10715



10757
10723
15148
7011
10145
10555
10562
10885
4615
4614
10592
11306



11308
10950
11366
10981
11484
11491
11061
11066
11524
11067
11589
11559



10940
11337
4867
15696
4771
15762
9196
16637
16636
9226
9230
16803



4936
17828
5784
11301
10012
29367
10763
11274
11399
11435
10139
10137



10135
11305
11363
11368
11409
11440
13737
22631
22635
14535
22638
14593



14597
22671
22673
21171
21127
21028
21175
20934
2618
2648
10795
10798



2619
2676
2675
2677
2679
5524
2681
2780
4637
3564
3605
29322



29325
4600
4598
10792
10790
5599
5600
29374
21064
20944
20940
20937



21061
20981
11767
21126
21092
21020
21069
22000
22029
22032
24069
24083



24120
24194
24086
24122
24117
24119
24158
24182
24186
24188
24153
24216



24219
24126
24244
24246
24249
24251
4774
4701
19529
19581
19532
3151



19583
19617
19573
19536
19576
19621
19661
19578
26326
26323
5703
19524



9634
14630
11338
10945
10937
10933
11094
11535
22003
16871
23011
13888



10687
10759
10725
10691
10751
23322
15023
16600
24154
24080
30427
19348



30456
25633
14773
7124
7178
7157
7150
7246
7182
7255
7101
7245



7215
7094
7118
7210
7156
7153
7286
7220
7186
7293
7249
7125



7151
7288
7326
7294
7296
7329
7333
8052
5631
3602
3600
4390



4392
4393
4385
4446
4452
4449
4422
4491
4492
4495
4517
4518



4547
4559
4555
4550
5523
5520
4718
5529
5560
5619
5626
5622



5601
11610
5667
5673
5669
5635
4457
4454
10732
5675
5678
7123



4852
4693
15678
4735
4821
15700
4736
4737
4741
4770
4773
4776



4806
15757
15786
15788
15791
15812
15817
15840
15842
4825
16603
16634



4856
16647
4860
16669
4863
16737
4894
4899
4900
4903
16778
16801



16807
4929
16832
4932
16867
4939
17668
4969
4973
4976
4977
17722



17725
5746
9982
10007
17788
17794
17800
5753
5757
5783
16703
10014



17832
17757
16772
16706
9973
9976
16739
17708
11576
11608
29646
19212



29648
29650
19215
29652
29668
19219
29669
29673
19240
29674
29676
19243



29697
19247
29699
19252
690
20263
29702
19256
29703
652
19284
642



20220
29734
19285
19287
29736
29737
30422
19322
30424
30426
19329
20265



19350
30446
19351
19352
686
20223
30453
20169
30457
20173
647
20228



605
608
16891
9952
10009
17765
9177
16643
9979
9983
14045
14016



14039
14040
13976
13974
13980
14012
14993
1358
4588
4584
4571
4574



4578
13670
13711
22598
22601
14564
14562
22667
16664
9182
9188
9223



9950
9954
10004
10761
10667
10688
10717
11500
11507
11505
14991
17532



1647
14787
14781
10730
13599
20410
2756
4380
3612
29364
29331
4522



4519
11579
11601
26325
4631
4630
4633
10824
2657
29369
29372
10693



4542
4540
4690
11333
11224
4733
4612
1900
10729
1898
1893
1896



11599
2714
5565
5563
5564
2721
2752
2723
2747
3571
3573
3569



4413
4411
4416
4420
4489
4485
4629
4603
4605
4660
4663
4702



4674
4669
4710
4714
11582
4704
5526
5597
5567
5596
13979
21993



13987
13992
13996
12409
13942
14015
1360
21231
14017
14782
14841
26664



11552
18066
18134
18070
18101
18206
13236
2768
20087
7036
18946
18255



18945
18264
13982
4581
4582
4908
11244
4616
4621
28178
4815
9947



9192
16893
18369
17217
14632
29645
19209
13830
14636
14637
13919
13914



13920
13917
13944
13945
13893
10689
21093
13854
13841
13847
17547
17545



20904
20906
20899
19127
19155
19150
27482
15098
726
2332
10069
17569



22026
14028
4404
4544
2336
13885
24342
9373
5634
19773
9371
16355



12698
14876
2402
22790
17017
940
939
10096
12386
14050
14053
12408



13921
750
749
782
745
747
23094
13889
1437
9150
15701
15718



16604
16769
4942
9955
17675
17719
5750
10034
15754
15676
14020
14046



24075
21228
13946
13951
14044
21225
21215
21221
14024
21178
21130
14818



13135
14805
14808
4966
15784
7918
1366
1369
1505
1403
1396
1475



1514
1389
1436
20903
14048
11618
10831
10827
10832
11612
20983
4694



11282
15719
15838
16670
9229
5754
8876
25306
19624
11594
21987
21997



14640
8051
649
610
20183
19318
715
20268
19159
10713
19358
19475



2718
2719
23072
23044
23046
23077
13891
6915
6774
6817
6790
19354



19357
13705
13708
13710
13714
13716
13747
13739
13741
13743
13745
13781



13782
13785
13789
14809
14784
13828
13833
14817
14777
13835
13840
4611



7376
7096
7374
7373
936
934
21135
20975
21169
21087
20978
10586



21134
20935
14270
13778
17534
17537
9633
20901
4832
10134
21098
27949



514
28711
16920


386:
7882
12624
24648
7567
20731
29246
13557
24299
11349
26482
23197
30121



11007
18272
6064
28273
8156
23323
18696
2199
21366
18611
817
13520



29728
24844
25005
12000
7747
11457
4078
4318
2050
17518
9735
11098



6203
22233
10599
7752
15287
23469
26750
24405
1630
26154
26137
14899



25769
7610
7037
10086
27409
27407
17049
17151
20444
7523
25492
28406



15590
10197
23316
3750
9054
28523
4158
13211
28948
26806
30382
9535



25501
13533
15796
29318
26382
14370
12937
14574
9931
5799
11078
13746



10020
20282
6416
25298
5849
29006
22869
17306
26462
14226
16058
14263



17573
14786
26451
28923
9374
4429
25618
22773
28275
12865
20976
27294



828
8708
26360
9275
22574
22262
30310
4283
18068
4968
24285
25256



28977
5519
15217
5412
10884
24397
17694
25575
30088
23404
24081
16839



5107
8862
20079
17553
6918
7696
12535
17038
8997
20832
10223
9073



18132
29509
5478
11312
1409
21181
26012
23113
10978
5297
11298
4964



17101
16605
20370
24570
22259
6108
10236
12212
7445
18469
27353
5467



1077
7762
21239
26187
16131
29514
23591
11815
18999
8501
12139
5767



21383
2686
29757
11839
4301
3462
11447
16522
11623
28752
3179
14591



6976
3373
20987
29125
7401
10219
7317
17818
15097
26598
26704
20088



23312
10793
18246
17592
14273
4691
1993
14136
27321
20931
13690
10369



26931
9853
28138
26603
11477
15496
13725
14514
13407
10283
29222
11832



26502
26670
9114
1924
1412
28350
5281
16091
19855
17086
27176
27611



25357
21603
30077
26906
8350
22679
18673
9140
18666
28921
23251
23519



20361
20770
25453
20837
29966
27231
15403
28609
11201
11848
25518
16868



22889
29735
17124
8175
20739
20369
12154
28580
8190
15884
15044
21154



9315
9642
19788
4835
6578
1253
8773
15685
10431
5352
6871
25767



14950
19775
5114
5589
17452
8608
27854
10987
21757
29392
8503
8481



12839
19718
22926
9040
14900
7830
29421
9537
6500
16012
23512
25104



656
17645
27804
15352
9712
5913
29582
26892
13949
15176
11476
25094



2764
7380
6284
23799
18323
27153
25398
3341
11241
18735
1521
22016



19647
18401
21554
20513
2805
17878
1940
26070
7663
3610
6563
24008



6643
12824
29049
5076
5078
13223
6440
16674
2417
22702
14743
14797



25787
24474
25771
15181
25271
28750
639
20190
18757
991
24602
11311



21751
18315
23688
2908
26458
26270
5283
3627
25101
15350
12381
25545



12033
9795
16156
15164
19152
1170
3238
4739
15254
9262
8304
27537



30349
25524
30092
11304


387:
4558
11893
13421
5582
23804
26468
26466
20275
14838
14839
17735
29084



18188
29076
15600
30039
919
11408
901
8685
2371
9440
3547
7111



8276
25471
17939
30262
29401
11076
10207
16596
21431
13490
7553
11422



21043
4627
5438
17143
3476
4884
20375
14672
3777
11489
5053
24683



24466
17525
8594
9714
24798
16490
28159
28437
27535
23196
23263
28941



29147
3370
17964
11414
23349
27411
29195
20137
21695
21637
20399
20446



12487
29234
6701
28295
3357
26876
13268
12016
1402
25239
27993
9006



26508
24009
9842
27164
9843
8093
14174
14907
14180
8251
11223
29131



14904
6743
26915
26914
12587
11766
3977
27351
13842
13838
17934
10791



11651
21246
17945
17022
10818
13428
9709
24623
16581
22949
7546
19064



23636
27599
24670
29538
26358
26361
16328
6121
1030
7784
4435
20083



5003
4716
791
3702
7029
9167
28702
14596
14556
11538
24982
21152



9549
15247
17111
16632
17709
21966
17305
16424
28354
28724
27364
24574



16426
6348
6280
15221
1997
12742
22001
15753
7248
12877
12875
24109



22403
5450
6999
22405
1130
17140
29251
7185
1810
3076
28353
26874



24295
29943
10644
1049
12979
14037
21570
18712
15540
8936
3161
4570



4539
4465
27883
12020
25071
28733
9892
29788
1819
29812
11598
3410



9138
2585
23556
28874
2934
12374
1664
29691
29088
3921


388:
16278
11063
25422
1816
11982
26099
6004
16365
28692
20122
20303
26654



786
10182
4508
1343
20478
7988
24286
24386
3094
25259
20056
18347



19690
23376
6317
27472
10522
30317
30020
22656
12232
4531
12165
28455



10913
19443
4173
9125
24324
30454
2037
9909
15010
15153
25931


389:
21076
21074
24054
19303
4397
8433
9597
13681
25701
8529
13682
29417



3329
6368
1930
18796
23129
8137
27913
27943
3570
15220
1921
23484



26097
10322
15273
21458
24501
30004
1058
17126
20178
13780
27821
21499



11343
2474
2429
23148
14120
1136
28029
5188
8301
2074
28682
23207



25234
4592
6577
15018
25183
20104
25311
27014
28022
2407
29658
27137



21835
22946
4286
2453
23771
1121
15152
7508
18125
14815
1806
16918



22338
8216
27934
28665
22072
14984
16297
19962
20824
5496
16107
10178



28394
3392
3438
3668
29062
28435
7617
14723
26147
15111
6330
5903



5261
12168
13265
20572
669
29113
8533
12630
12759
5382
29134
603



14259
15218
11176
19111
16904
4137
30155
22315
26211
5171
4474
13423



699
26857
25889
15695
5656
13452
16624
24229
19905
19885
12708
13922



18917
26895
14843
14806
24003
20069
6597
4337
21353
24639
8520
9720



4430
1118
20387
24437
3181
3281
28201
2323
28358
10379
28321
7579



2307
2278
15208
2901
8613
21511
5811
10636
18192
13404
28598
1556



616
30406
655
29004
28129
3850
9148
20126
5502
2166
3650
16685



26728
11100
9014
20231
843
845
22705
23137
7834
6945
5583
30396



28421
1577
27492
11302
1987
30225
6290
11946
14973
840
13085
13054



27292
9981
9959
28958
3716
14277
2285
24091
11256
28742
28741
15833



21106
24036
26077
23993
20117
30096
10683
21582
21738
23681
21984
21885



13678
25157
20887
19948
24070
27527
23997
16961
8064
29845
1884
9986



7187
9957
7894
21587
13384
7772
26614
12745
27838
22022
22147
15901



18009
8985
7805
29129
23495
20727
8101
8067
6335
6333
22062
24049



3494
2983
19995
18749
3037
18015
5211
29352
9366
29282
9452
1093



28291
24425
17497
29099
14986
7133
29551
12227
12228
24624
24420
22559



13301
8373
9341
25695
24780
13044
6000
18736
24773
15831
26257
24398



2928
18271
19938
8219
18147
28191
26595
18550
19355
26346
2776
12184



28768
23707
9255
28548
7459
22182
17454
5995
16136
9213
8471
2315



17099
29529
3898
12065
3718
1743
10595
28519
25835
28283
13197
17310



21045
8375
25339
21500
24781
21655
1200
23953
28006
6543
9145
22710



20132
12235
16370
19810


390:
22423
29361
12542
16648
24697
17759
12099
3299
2596
1967


391:
16278
11063
25422
1816
11982
21406
6004
24783
16365
20122
20303
26654



3249
786
10182
26408
1343
20478
7988
3094
25259
20056
18347
19690



23376
6317
27472
10522
30317
30020
22656
12232
4531
8668
14561
14464



29649
11699
25874
12165
15149
26411
28455
10913
26414
4173
9125
24324



30454
2037
9909
15010
15153
25931


392:
12517
6596
26601
26683
5868
17245
28699
13374
16403
20008
26900
25162



14567


393:
28605
4248
3296
29412
1544
4971
25372
29811
1043
22936
29875
15017



12913
14832
11897
7444
7783
1580
1575
7449
27975
1736
10963
18607



5012
4790
19491
26181
26164
26179
26166
6084
22822
22119
11926
6143



18802
24675
1642
22297
12158
18339
19224
8729
8745
12629
14650
1776



1741
1707
1778
12954
12140
17986
7486
14323
29298
6358
30249
7435



16020
7899
28663
18034
27243
3117
16040
5952
12823
8778
27116
22716



5812
26114
12292
27504
14526
25559
26246
15865
2010
25596
8728
28654



24775
1378
24778
14306
24842
12932
25247
10314
18165
7012
28721
21714



19012
6704
25342
19555
29166
25312
20859
13168
20643
10542
7494
20348



24013
29672
30463
1523
9707
25278
30341
26500
17219
28319
7490
8545



29013
27753
9225
26782
21073
23700
23703
6862
18520
30018
12861
4577



15163
6845
3397
13969
1984
1624
4624
26478
30397
28565
17213
12054



12089
10866
17773
23206
29565
21514
1401
11549
28292
23164
4926
23911



4949
11088
10104
16644
1094
13688
6671
27258
3303
15049
2352
2991



11713
19314
23318
8230
24947
27463
5484
30384
1163
17893
1416
9330



12405
9333
15391
2936
8998
19519
19525
12156
7757
28818
30141
8398



10740
15295
20325
17325
5730
18713
25031
2365
22116
25257
22657
29389



28650
19315
17650
30443
20699
7510
7155
6448
1112
12915
6331
29239



17760
17835
14733
25882
3979
21962
17861
18807
6030
23278
24272
29330



27365
17805
8719
13173
12233
22827
19739
18618
18706
13658
4700
23121



24243
12467
11326
26575
26573
20560
4045
10190
25319
26814
27915
23651



707
12290
9084
9682
6494
3914
15760
5693
20537
26901
24888
6684



22797
22636
11814
19188
29380
8147
19574
30403
20874
10580
21715
16700



1474
26695
24247
25000
9657
18453
30101
23513
19849
24716
23516
1946



1947
19018
26271
25477
25484
26363
25473
25441
25410
25409
26442
26406



26437
26435
25385
15727
15923
20963
17949
17789
17795
26324
29456
30066



26418
4838
18314
19403
15957
29036
6998
8654
6240
26356
28440
9269



17028
10474
26202
10677
2087
2800
28132
22715
6476
20654
4987
15578



6289
3621
2930
28144
27716
21519
21613
14654
25403
7163
6716
28476



10944
1063
17188
5320
17158
26089
22149
5983
8791
8789
8788
28070



13263
6861
19701
30278
19126
15936
18120
20620
4222
729
12941
18345



10382
12252
9624
23119
14949
12867
907
911
13144
12938
730
14135



3636
11786
23990
27897
10675
7456
12498
1820
28552
10722
11720
19506



20979
774
24722
22877
7362
18911
10471
28039
23542
22349
3768
13202



16598
6715
6484
4027
11381
2468
26746
20475
8622
9998
25324
14855



27500
10138
26954
8111
10504
13274
8108
22554
26174
6009
12944
12936



19730
12943
22572
23515
6827
10166
28974
24489
20584
21430
19541
26962



25404
1595
9869
18282
24908
23226
20984
27679
25034
30447
9096
16204



4650
8044
7260
17195
12935
21446
19526
20129
3842
17736
15276
2794



22503
3051
5227
11362
4113
17220
15966
7488
28369


394:
13283
26673
13372
15811
26338
10282
13817
6554
14047
11667
10532
2214



1074
7236
16820
12807
3295
23864
2601
24596
16280
17983
25511
29357



25240
26751
12803
12244
7105
11527
26908
20080
23916
10142
938
16901



25535
19825
15340
7736
7452
27777
19857
3690
18820
16411
29415
6075



13087
15907
4322
4242
17935
4377
30307


395:
5925
5923
30049
5276
646
3497
16821
4682
4024
4026
3989
4022



4201
3419
4089
11871
21479
11456
22786
12064
16900
7864
7137
27519



23009
22962
27386
5910
9782
13669
5022
5922
28232
28240
28245
14945



2384
26153
9863
17122
9701
4513
24276
24303
14985
11226
19391
7568



7623
1468
5050
5147
22361
8683
16205
16243
16210
16234
16200
16272



16237
16201
16178
22362
16267
16266
8681
16202
16269
16288
16274
16263



16207
16241
16295
20101
18903
18977
3714
2312
18880
3684
18869
18905



3713
30399
28299
18939
18972
18846
27074
17528
18898
18848
30321
23708



19003
3689
3662
19026
18935
27273
19602
2620
27279
28024
18645
18667



18669
928
20551
19019
14467
18251
3846
1656
20430
4364
30027
20555



11096
24440
21580
25207
29141
10133
11590
5354
7744
7942
17790
2061



18662
8642
23923
27214
15651
23078
14362
7927
10619
1018
14617
25685



12777
22187
11042
18732
12198
29306
5504
15836
9171
30044
6414
25669



13863
24429
11873
18064
27521
4646
10130
20051
3139
25845
30336
10377



9691
5577
30414
1012
24812
5492
4428
1204
26650
18168
18283
4355



21394
5462
4099
4071
4122
9325
15857
4125
4127
10368
816
814



833
8020
12594
11867
25573
27585
27146
16588


396:
10985
24465
5389
21752
27373
11218
19349
8484
27565
9644
15930
18320



10788
1620
7764
5228
20488
8231
3344
26554
18643
8540
22162
6528



5847
14377
7956
11564
7665
30212
26544
19808
27823


397:
10015
28467
9949
16910


398:
14943
7938
7964
7962
19565
11649
1841
3138
18175
16084
28102
25813



4265
27430
11048
5711
13120
2802
8841
19091
28086
27114
3741
21369



19052
1394
21023
927
20385
28263
19113
7946
7941
28293
4583
3706



3705
5387
4585
5998
1847
1844
18466
16361
28638
27344
7934
11733



20519
14569
22469
24958
7905
7870
14980
580
6150
9513
28956
12098



18280
9800
24457


399:
26644
4656
6625


400:
13109
6571
9398
6996
22385
12637
8380
28556
20052
30329
13485
10400



27196
27193
15191
20656
10460
13771
3445
16498
12884
12318
3470
16845



20269
5461
20330
20335
24308
4110
931
20492
19564
5770
24535
4499



1881
9120
7450
18506
18374


401:
19639
6086
22364
24585
25042
23621
30345
28252
17067
23995
9310
22994



5072
16172
25659
1771
16962
16937
8287
28032
28064
24087
25025
24400



24269
970
14519
18442
9210
7765
21881
1530
26626
3698
12231
18434



12830
13547
3947
11008
3887
4234
4319
9555
18366
17971
28689
20851



19953
6180
28096
29797
13032
25388
3829
12069
11806
16075
26665
12135



3790
11742
25940
28091
9662
28734
21963
29545
21722
7781
12477
7536



29955
25777
5118
9304
15780
23706
14555
13156
18084
11085
25550
28520



26030
15158
16126
9147
14340
25656
30430
27229
6216
18028
10276
6531



25120
4464
28347
25326
5415
5702
2194
22060
25588
17643
24707
21709



1406
19556
12441
11544
2972
18250
7310
18048
3005
5805
29963
9130



27259
10371
19075
7197
10797
28371
6145
7878
14446
25400
29587
13473



9117
7469
8935
28237
1268
5108
27560
30429
25279
29310
1971
9953



27476
6140
2328
3186
1062
7447
28183
24438
1880
29932
28813
7346



3575
23091
4502
10351
11558
27380
26117
24357
16376
18325
12600
30196



11062
26767
17070
8022
28167
29891
16350
16423
23738
14399
17149
11103



18776
4471
29877
2963
22453
23060
5677
19269
27154
22938
9041
21540



16319
10029
7970
17888
22885
23452
18578
22148
6455
27794
5238
26759



14078
28332
6196
3954
9208
7314
28148
5595
8962
26277
19037
2014



10201
8980
7269
17510
17508
11702
1422
27709
9030
11694
14189
2206



29095
15026
21970
28753
18638
12949
15782
23500
29566
19561
8922
18888



8635
27031
7730
22273
26855
7802
29170
28338
12192
1857
20955
651



22972
24396
11729
22455
22464
18763
22460
18762
22487
18739
22467
22485



7470
7471
11693
18710
3193
13348
28870
15039
9841
5648
20114
7565



12240
9101
5901
20829
13698
14604
10081
20528
2863
8077
2416
4156



2415
27851
5551
28791
17075
30460
4848
2262
19771
25640
23109
12887



6142
23733
3666
27756
6285
26283
21176
935
19132
4843
30126
22186



4284
25896
18049
8295
19156
15981
1011
22968
22088
9857
14290
23049



26397
13284
14418
4602
24020
6118
1395
23400
23507
21706
5367
19834



8873
2302
8667
15171
22085
5729
16959
23940
5011
26616
18548
14054



10262
5150
12512
25370
15464
13513
18053
17598
13448
14364
11233
11270



21656
11011
22908
30151
8955
22425
17923
3380
1438
17594
19013
27621



28389
28913
7821
28912
2786
11164
6678
15747
27497
20266
26776
29512



28226
11971
28017
4008
20628
24450
3536
13603
8954
15550
17185
24310



28797
23569
4473
20625
25921
3147
21089
14413
27638
5831
28480
1539



27117
14704
3725
24113
28453
24936
18521
22134
4546
1552
12470
30034



27520
23954
16139
16135
21623
24776
28775
28055
28940
10264
27628
12480



27627
27654
25661
2259
25343
16930
7393
19140
13385
20057
8377
8740



20298
23308
29768
12802
17381
1340
3519
24380
15106
23114
10043
17563



665
11696
28774
3712
28052
28795
3710
28803
28004
1100
1745
6823



28059
28007
28821
28831
28828
28011
1056
27973
7917
20110
20111
11689



11735
11660
11691
11688
11656
11734
28885
28886
15549
12475
8397
11726



11727
25525
28802
28800
28050
20013
7169
889
22332
15319
15635
19445



8489
2563
5224
21062
14388
4477
8449
13099
25576
21311
13238
12634



18024
8959
23255
13076
815
27247
3102
19128
16024
15512
6801
16846



18133
28443
20053
8193
10003
3852
29972
24550
17915
23552
4315
4280



4387
4277
11738
4275
4360
4233
4358
4229
26798
12704
8354
22489



25836
5697
7788
23578
24327
24329
3364
3402
3399
3396
28914
7718



7713
29806
15542
15541
17839
28097
11663
11455
11459
11454
11499
11496



11664
11654
11509
11652
27004
28725
11740
18772
22365
16071
5570
15151



6005
25563
15361
414
2505
20554
4205
4202
4162
4199
4164
15024


402:
21731
19306
27634
10169
11204
28554
2003
8294
19828
26964
17294
22015



26278
28659
19087
9113
12024
13846
12988
10512
22492
11099
10957
5104



6103
7321
15346
2280
2464
29656
18958


403:
23201
8902
17383
26008
26005
9941
5941
28758
17348
25230
22478
22649



5636
21104
21891
6072
16847
13755
10088
8809
14343
2411
23363
20102



2593
10779
26745
24719
2096
19888
862
5398
2646
28899
17727
8324



7614
24127


404:
22505
17330
17332
3832
26661
24604
24601
26617
26553
26585
26591
26640



26582
26471
26635
26572
26576
26556
26609
26423
25650
26551
2373
2405



24690
25446
25450
26422
26473
25340
10489
21242
21238
21241
21279
24723



24725
25494
25690
25164
16529
7978
10037
16615
16621
11055
12923
6955



7874
7948
7839
8664
12144
14757
17096
14796
14828
26829
12171
21124



23048
20000
23191
22333
20933
15625
22212
19851
28192
27896
22998
22992



15629
28137
24133
22151
29931
985
2060
944
28955
28930
28990
29965



15658
21486
21255
20071
19073
19014
18158
18127
18109
15620
2239
2233



16398
14851
11657
12588
11744
14570
13748
13790
12143
12170
12173
12919



15622
15628
15656
15604
16393
16394
16392
12916
11037
22432
8701
24656



19135
19853
7817
18495
2869
8263
29437
29438
22440
22435
21711
22465



21146
17031
19010
21907
27742
22415
5429
1188
5421
18365
23435
12382



12090
12087
10571
18362
23640
15382
987
6445
6417
5413
8888
2538



24892
15423
1089
1127
5417
21701
24630
24633
21702
21705
25589
25558



21697
7438
22989
17339
1478
1506
1479
9199
25171
26307
1472
25424



25418
5394
23942
8884
8887
12914
23541


405:
17859
1238
22054
28433
14522
12713
2265
30161
7453
4765
16339
3623



16249
30466
23897
24523
18199
12780
12018
8775
20575
9860
9828
17997



22323
12760
6777
25696
8048
7233
7159
10567
11570
5422
13420
743



20960
20777
13662
6471
6450
722
1999
22130
15016
24746
17989
21018



18525
29549
4744
4911
15147
16008
14299
4655
18309
20204
13162
27357



28445
29670
12438
2514
2700
25507
27408
21700
6258
15916
23219
8612



21584
7769
21090
21432
24336
2356
30137
4195
24837
12189
4171
20221



23536
26716
7664
11285
23505
6454
21901
13438
27127
6046
28726
23456



15186
1641
24978
18008
19271
13956
24528
17271
1119
18682
4177
14894



2489
29476
22053
1277
2172
26999
13676
22871
16889
8678
12883
23881



9761
20677
12855
3738
22399
20134
18998
18171
2684
15899
7332
29308



9846
23454
17170
1559
28849
2839
27226
11010
10112
1021
29279
19520



19722
26879
28081
15983
17146
15924
5208
2143
4634
16484
3406
25815



25097
16791
9830
25112
1379
28559
28606
16292
16260
16208
16320
8188



28196
5518
5513
11332
7484
24098
25192
11722
7295
4070
3808
20017



21780
1418
3680
9910
7166
23057
22218
23084
16029
9268
11075
26038



12485
7431
18804
6947
8806
3674
28046
5006
28297
7847
24808
19618



22706
21561
4782
24417
3843
24691
11449
9680
30005
10365
4403
14746



20774
4849
29201
28648
20671
18342
23463
29918
24863
14394
9080
25560



8423
7517
27591
30030
7571
29151
7460
3064
8500
8416
18919
15655



22879
22882
14872
2032
28860
21021
11112
2577
10386
7462
7356
9905



21034
29197
8891
6388
15308
29956
25999
24693
19760
18166
14979
10427



18403
23159
5100
26531
26168
9132
29747
26986
7010
6925
9803
26555



30057
7883
20362
13923
21199
17720
10376
13693
28462
5696
15243
4681



11036
28713
23116
22490
18697
15953
16090
8882
29422
26513
10652
10658



14981
6819
823
19664
14931
2181
15988
17595
11629
10661
12706
6147



6518
28422
8191
15040
11675
3972
3697
28043
5953
21288
17280
10246



18586
18588
19908
19910
15765
26810
19134
17663
17697
3638
21052
16371



15495
20728
421
2409
22097
3355
11327
6847
1500
24226
15048
13579



1887
8939
8964
13794
9157
24816
21858
5885
22336
3805
3802
24169



2292
26734
26770
6657
13127
26043
12384
16093
3544
14897
2567
27765



27766
27677
15125
20964
23705
23066
24145
25943
24093
17443
7635
1827



22760
29245
18346
14261
16514
16482
16233
16542
18557
9646
13061
28991



23979
2413
30304
24393
6062
867
1186
8879
3327
2945
11059
22618


406:
20907
20913
3191
25797
8462
1879
4652
24363
24360
10871
12810
10583



10437
19450
18493
11510
27173
15212
15214
8588
8590
9632
24462
24484



28260
23999
14749
8029
12739
14780
18654
18652
2625
8464
5633
5628



27588
16686
3828
21787
21790
3125
13953
3308
4204
18019
7272
7273



15636
28388
5136
17046
12262
11394
8268
3615
21005
16635
4983
7531



30344
1135
10374
5166
10372
18447
18445
24095
21224
10039
10660
13773



13775
2682
15088
15091
17289
28854
28855
4389
6838
18118
25300
25284



3909
19553
19550
9896
30410
6350
6352
21644
4235
14421
1655
1687



11225
4512
25728
4126
13936
2284
9577
2434
9351
19189
736
738



19080
19078
11886
14543
12731
5616
945
24661
29479
29477
19310
15449



7264
7262
16117
8641
9679
13198
23382
16013
13897
21305
9361
26329



25272
13326
6626
25686
24764
25861
11330
20684
25383
25380
19817
25252



15330
15329
15599
19590
19588
8432
8434
6633
6636
7787
8665
22993



14957
24628
15222
24197
24196
10768
12515
9666
21632
21629
29385
7904



24043
27042
4463
8488
7594
18889
8468
7705
16095
15379
25365
1105



1256
9781
9756
24514
13875
13877
29068
29070
25581
26936
641
18655



13276
17422
6035
3456
10575
10285
22383
18810
27581
24301
19856
23293



19774
14585
13837
18045
12415
28809
1719
6283
3275
5721
18107
18138



14770
4010
29287
28310
24971
19974
19972
25303
12114
23538
19092
18207



3401
8653
18020
28233
10290
27800
13497
12795
21143
24208
10362
14358



30035
26498
26480
2579
2581
25553
30468
19029
11898
4098
14169
15514



7910
7526
13171
27054
12790
1986
12789
23005
19231
2976
15569
20232



15921
15922
23759
23842
28666
18692
18690
23244
23407
17423
17427
2897



27714
25824
25996
7047
2917
12909
4107
4108
21136
12211
2611
3592



10480
18755
1028
1083
1963
22608
22456
29314
12417
28403
1979
17156



17153
13292
26514
19436
12547
19440
9160
14472
23872
23739
29989
1335



14762
15717
8060
8059
23117
29375
29376
26898
26902
18701
18704
29253



7320
30440
30441
20734
23837
10657
24002
24001
20961
23981
24460
19850



3286
25065
23832
17487
28157
27536
27533
4371
15156
17678
22952
16046



5165
5370
8837
21890
19970
29406
8021
23986
28783
28553
5644
5641



21285
22940
22969
13328
28250
4134
28550
3444
26567
21414
958
8881



12551
1716
17811
17812
12712
13880
22545
22546
3504
3506
11188
11214



17887
4937
3552
3551
17399
17402
10773
8411
5793
5318
16770
9716



17927
8463
27561
29358
11998
27717
14721
14058
11597
28740
2871
26354



25451
20098
7924
12550
6100
24279
24277
20396
2049
1065
11232
21892



28207
25149
27734
6497
9417
26597
14974
20985
12570
20521
18886
17886



23304
18904
19175
1387
29485
3384
16225
11238
9062
13183
17080
2487



2491
27726
29848
3609
5598
22808
19182
24278
16173
8744
28044
1695



3387
15194
27618
7048
3389
29713
5176
27037
9625
20426
29634
29188



18873
18872
25945
27822
16344
11957
28964
17260
18190
24580
5580
6177



17614
29315
23000
29323
23763
27598
26802
2068
8800
27178
22450
20363



19397
16749
24564
22557
24273
24092
11197
13251
5525
11883
12610
8401



5939
3180
17437
8733
19094
16076
16080
13482
15213
25017
14352
13469



24642
29487
23655
19380
5916
10810
21784
3872
21783
10211
14168
20997



3827


407:
21969
3426
23405
5511
8336
7035
21611
12774
12091
28615
20024
28014



12005
3736
28490
17277
6567
2642
5615
22527
23461
23548
2510
16657



14727
18948
681
14155
24237
9719
27107
2157
12368
4881
21443
3211



6740
29290
12934
12791
15334
6320
12678
2665
7340
27172
25880
13369



18111
20334
20201
24004
17227
1996
25095
15305
2617
8320
14635
5475



24706
20420
5892
20894
8105
9553
6246
22041
14153
27670
20341
10564



27658
2795
4144
6083
17408
6067
23247
16218
457
14021
12930
5230



2316
26146
22112
8274
19356
14025
379
10900
3477
18809
25408
19607



21727
2858
24353
25045
28887
5143
16074
9958
11336
4365
18650


408:
8062
25546
1621
1614
3009
3717
29164
16148
12892
13560
16717
22076



27209
9526
29022
8028
19060
6866
9859
9521
7039
26735
8846
1715



1672
28893
13883
2631
6794
16995
1850
6858
14668
28859
4514
23601



16164
6975
12533
9365
9172
4159
21035
10240
18547
5671
11514
11548



5676
11550
5680
5674
6470
6468
7842
12045
5223
8553
3704
24541



4607
10019
18105
29082
29077
1410
15795
28994
29007
28915
29042
28911



28939
28963
29040
28907
28213
28212
29071
29037
30138
29832
8948
2225



5778
20322
28399
16532
28220
2469
4696
15116
16142
27979
3696
2867



12965
28477
3280
12359
27750
22948
1258
5427
5460
18149
28362
4902



17917
13498
14814
28150
24160
15483
12750
17479
1662
6433
9794
1592



15146
9280
3088
12015
4354
20546
24902
11555
4335
22457
16123
17558



17717
910
10438
28145
6908
10708
28408
5252
16374
26917
9238
8861



24620
23131
22834
26229
28302
6235
26899
7779
4266
29616
1606
1609



1467
1691
1659
1689
1661
1694
1501
1476
2647
2750
2746
1727



1761
1734
1728
1725
1473
803
1587
10610
1574
1579
1572
2811



1541
2561
2586
2589
2604
3580
10615
1509
2651
2742
2739
1768



1763
1612
1651
1613
1618
1654
1583
1543
1537
2527
2530
2524



1582
23525
19216
29085
7774
2331
23154
29609
25022
25381
23955
30244



11367
9700
22351
15912
2551
3728
13514
17240
7818
21759
10429
19954



10825
21007
15393
21331
25505
7916
9568
25938
11553
10131
3137
9322



9027
3962
21216
11719
27112
30197
20407
2703
30094
10217
14312
23485



10245
3529
12563
27224
10266
28943
23369
9552
22613
18513
21022
5606



1546
1548
1573
1578
1586
1590
1608
1611
1617
1653
1657
1658



6919
11797
11827
4451
19065
29618
5285
3288
7613
660
8693
27839



2810
2553
18726
7901
24270
9299
2519
16420
6984
15297
18483
25174



26789
23155
22686
14076
11152
16256
23295
20948
28788
22250
5411
3126



25705
25265
6921
28113
5057
8743
14281
6158
19273
19478
10433
6792



17640
27860
27003
9451
12592
28271
29712
22575
28981
27857
4096
24847



12257
27013
8199
18182
20431
15357
7763
3491
24846
6182
18010
25888



25407
25088
21588
16819
28637
24389
11216
5543
4135
7475
8609
22226



24673
27255
1489
15644
21618
13285
6198
12085
18071
14682
13139
1415



17807
17061
13474
13119
22434
6677
16352
23720
8083
6895
18930
28697



24175
21438
5025
13598
6340
30086
17974
12976
25371
28537
22086
10711



5962
18451
3617
1645
16773
18011
7668
8086
8075
8073
8445
11782



19317
25997
25868
20481
23914
23917
23120
14111
3590
15442
9295
27636



11966
7992
8095
14960
27986
24824
1538
25046
30318
28351
15917
9687



10435
9187
20535
1599
24522
2542
19104
16153
2680
23937
14540
15961



12126
2020
20693
26760
11857
1513
11794
4643
16921
21899
17288
2017



7349
8476
19382
17169
13154
10448
913
11040
20243
13186
13142
13116



13151
1753
14453
6037
6586
21167
17127
7728
14141
9472
14953
15731



20138
20246
20354
13399
981
20198
796
1710
7487
1596
20288
723



1471
11035
28184
28181
28156
28190
28189
28844
27928
17042
4772
1151



24320
18465
13396
9570
20364
21253
20359
21250
20323
20157
20202
20153



20158
20240
20356
20260
20257
20162
20255
20326
20367
20331
20208
20317



20320
2605
2709
2716
2713
2615
2610
3587
3586
20193
797
20196



20290
20164
2550
30289
13056
28817
10607
29043
26783
29075
28947
28944



29964
26807
29192
1681
13082
7027
14335
25964
11058
4240
18756
14942



12023
15451
27741
17482
27091
12350
20736
5974
1126
25128
13028
27746



22847
22159
1639
13191
1751
13053
13124
13184
1643
20287
27063
7593



15102
29000
8297
7129
12975
20523
17084
22500
3163
1310
13081
1504



18430
25198
25679
25678
25106
25248
25175
25169
25255
25181
18459
18461



18435
25396
25394
25329
25362
25335
25320
25282
25263
25325
28975
14262



4747
28996
28999
16645
22944
8114
27237
13113
13084
13115
28318
13059



13194
13147
13187
13145
13033
13086
13031
13189
1809
25865
14139
15466



4851
1603
1633
1635
20284
20292
1747
1670
1748
1674
11704
14332



15281
28967
29044
12730
29730
7399
1708
9253


409:
13427
23058
5243
11587
23453
12652
21459
6252
7672
2947
5347
2102



5688
25571
26365
10626
27508
18241
12440
12814
19050
23328
2423
8167



4061
29223
10487
30179
8917
30231
2636
26203
18152
10949
28873
5376



3157
16589
21681
25670
30177
16958
8840
18876
19876
12650
29394
16675



15087
25201
4368
12753
6475
7056
17215
24280
20601
22335
8505
25901



21973
4263
28089
16284
24470
19929
22991
17198
8765
21482
13324
3652



17262
5517
4750
15168
2054
9507
4111
27757
20646
14592
19951
27118



11142
15189
9637
14837
20735
12436
4064
30293
18039
24028
1259
3440



10545
7446
25216
4415
11879
27148
26516


410:
17488
14670
25907
13589
14392
28308
12688
3967
27108
3325
11903
27253



24850
14090
16633
5804
8233
11276


411:
23960
23994
23991
24179
24180
24076
24118
24146
24149
24030
24181
24184



24209
24210
24213
24217
24110
24232
24034
24105
24236
24238
23998
24071



23989
24241
23996
24178
24037
24027
19800
24996
29453
11999
10858
30053



28316
7212
16755
20729
22270
7627
11355
12504
27348
20733
28622
3242



5744
13159
7477
4347
10410
10411
11339
10413
18220
15424
23615
717



719
19472
26754
20123
11575
29990
17924
26612
26213
26212
26210
14807


412:
14671
15692
15987
22073
5813
23395
3235
19033
861
4626
2988
10228



766
6195
16805
18479


413:
12549
18187
6723
6893
30215
24496
11543
17447
19279
30095
26452
14203



8981
10294
25217
11910
25862
23600
25330
18729
1209
22132
7771
19809



19840
1324
7421
28894
24897
15572
10412
10385
4017
10179
7291
7330



15721
3227
9290
4031
22627
22621
1295
23614
16939
1294
13096
14594



10826
23830
9875
14458
6600
11620
4115
7279
29579
6250
14102
12301



24256
18504
3243
18239
21132
24871
24482
9462
30400
27319
12028
23195



2195
2112
19258
27367
11902
1380
4207
1377
28739
28780
2560
2251



8457
30203
20391
27431
2912
11442
14775
24603
20302
20411
25165
16179



11959
10309
22529
14726
13772
6477
20236
5695
5369
19255
25035
28140



25754
13122
17090
17088
19199
21934
19434
16556
7415
27702
23319
8854



16044
22249
28264
10440
27671
28727
1022
26381
29304
1004
23153
27331



14795
14064
30180
5439
7076
20788
2578
20541
6750
7219
17952
17206



2016
29825
10673
2098
19307
28535
16396
19692
29355
23257
11756
8181



25454
3487
3490
7146
4986
21626
17743
17657
4306
30116
15904
22714



20998
23432
15302
14276
17895
11156
12032
30445
29816
989
26655
26656



24099
24101
4219
4218
28402
28336
2249
23351
961
3797
24100
1464



28397
24921
20495
25816
3359
13439
28969
9704
19131
19554
28472
19193



6493
3128
24287
29398
8945
29802
23794
9455
8508
6576
6785
21286



8869
9559
8824
8823
6225
1015
9878
30412
29319
16475
21186
13968



10743
12132
10002
14558
15461
11653
11655
27592
8987
9015
26600
22337



29254
16672
5661
23871
27946
8717
16536
2729
7175
6458
2730
19886



19884
26668
26666
3562
28005
27490
27944
22845
6463
4095
3000
14154



9000
3542
3458
17748
7558
5488
12500
8528
15560
7439
6739
3611



19323
25663
28683
6590
776
21017
21203
12649
14927
12693
14937
23123



15889
27918
14923
24230
11753
4148
19106
20058
6880
12738
10316
10405



28971
11945
21707
8901
17579
11315
15603
14965
11951
15789
27908
11068



25881
15610
8857
28019
30319
22677
9164
26279
23628
22792
12255
11290



20508
27661
13605
10261
22573
22577
22576
23487
16010
10061
24578
9544



26041
13967
27940
22051
7263
9033
29592
6788
16551
16002
10443
15858



15862
10300
15860
28315
8379
15207
15167
15170
15140
15137
15173
26987



16524
14204
14211
12261
18209
977
30115
10746
17054
11609
10585
22918



22916
22982
23033
28626
24555
25261
25520
9166
10297
30131
9872
8915



9272
5608
22842
11082
13193
1660
11466
13161
10196
10255
8292
24977



19456
24387
16983
25788
17307
14298
23921
24520
11546
15021
3035
19122



22397
8387
30428
12614
18816
29994
29186
22807
27437
26000
24509
24477



24511
24515
24517
8909
9968
19447
10561
19507
16661
12928
29264
5409



15284
8889
19268
27219
27217
26835
27423
26663
25782
8129
26565
7796



19841
28880
20239
27075


414:
28852
15638
17009
6683
16039
5647
28427
890
18228
6105
16172
1771



7145
10622
6022
2613
28259
29515
4357
8326
19653
12830
12478
401



8441
9141
18198
4527
17869
26532
24923
21211
976
17092
7122
1167



4885
8002
20762
23274
20547
25649
19161
10754
5179
6465
25364
3395



27640
24980
7840
29738
12477
17516
9474
3936
16980
19557
13703
16878



22607
25235
13904
13250
1047
13392
6129
30257
6509
24453
23187
26194



21355
11222
26605
6397
13313
10666
3577
8479
14823
11666
18856
30297



14116
7827
22359
7791
20046
25765
1385
26631
19200
21539
19116
28056



23396
16713
5651
23317
14513
25124
16180
15307
17556
27259
14768
8865



13696
18699
19395
11033
12605
28945
17006
16032
9880
24412
28474
25974



22541
22540
30368
30401
19370
14995
2369
13973
18212
11309
9695
9324



22518
23300
4831
3345
2257
9702
21449
18968
3503
24702
1266
12286



11393
21501
6804
25638
15689
21091
28116
20314
13050
13353
12953
19169



27681
25991
3553
27783
8863
21558
6432
3585
19009
8984
22488
28425



28372
20493
14706
15432
28301
15697
7947
22520
22749
20146
29086
25985



2650
29707
21537
20350
20618
16287
14042
7804
18046
6176
14412
27806



22536
22090
4038
16872
2541
6592
15938
11702
17508
17510
1422
3635



11694
14189
10388
2142
27706
23269
4150
9767
6994
9838
24831
1730



11855
4622
16400
4426
4456
18745
20869
24274
21316
5871
27263
25036



7801
14267
16965
24586
22741
28908
11729
22455
18739
22464
18763
22467



22487
22460
18762
22485
11693
18710
3193
13348
28870
15039
5551
7913



29439
9149
17075
30460
4848
2262
19771
25640
23109
12887
6142
23733



3666
27756
935
4843
28791
4284
30126
10081
15981
2415
2416
27851



7565
5901
20829
13698
14604
21706
23400
14418
24020
25799
14956
20528



26397
9627
20378
14290
1395
21176
19132
19806
6002
24924
8295
26523



1011
22939
22968
4602
13284
18049
22528
6267
9857
4757
6118
6053



19156
17418
617
10776
27467
18329
24475
12655
22088
13133
6137
5743



27955
11718
15232
14150
11737
12375
11728
11641
27323
4620
2147
4967



23049
25416
5541
15260
11047
13972
13504
1010
21041
4417
29749
5930



15783
7966
3003
29823
14759
19481
2779
27454
11537
22341
2883
5343



22493
22883
2906
6396
13663
1168
5729
5011
15171
22085
10262
16959



5150
12512
23940
15464
18548
14054
26616
19834
18053
8873
13448
25370



2302
14364
11270
11233
13513
17598
22425
8667
17594
22908
30151
17923



19013
8955
27899
5428
27481
4282
14393
25953
8707
1438
11011
2360



28214
3380
14004
21652
12442
9823
14608
16151
11314
7692
14481
9809



27621
28389
28913
7821
28912
11698
2786
16182
30108
28226
24310
8954



15550
28017
4008
20628
24450
3536
13603
28797
25921
4473
20625
3147



14413
21089
1539
23569
18521
14704
5831
22134
24936
18035
28480
4546



799
27117
11365
4143
8899
30102
4689
18234
15735
3232
7395
27043



14845
3725
12816
20902
25355
16212
16823
17436
25565
29860
3294
21972



20025
11084
27638
24113
3434
28595
12042
3002
14802
8280
22114
13288



7247
4188
30324
18791
1552
23915
25341
12470
16139
16135
21623
24776



28775
28055
25677
28940
10264
27627
12480
27628
27654
28416
27817
3098



3450
17563
11696
665
3712
28774
28052
28795
3710
19046
28803
28004



6823
1100
1745
28059
28007
28831
28821
28828
1056
27973
28011
7917



20110
20111
11689
11660
11656
11688
11735
11691
11734
28885
28886
15549



12475
8397
11726
11727
25525
28800
28802
28050
28794
27983
28773
10507



889
7932
9202
9772
25449
6644
9821
21194
13207
13099
16098
13238



15967
1671
26108
27788
26849
28704
24124
16660
25348
3363
1759
8750



22058
17197
12031
19854
4329
8752
12335
8926
14096
3870
2640
22222



21042
12635
6801
13076
815
3102
19128
28443
23255
10003
24550
8193



18024
18913
10475
20706
29365
8732
25064
7381
7862
4280
4387
4315



4275
4360
4233
4358
4229
4277
11738
22193
18443
28274
3364
3396



3402
3399
28914
4375
12215
4677
12631
11664
11654
11663
11652
11499



11496
11455
11509
11459
11454
16704
19282
16740
27227
11740
6436
10899



19172
8486
5666
21822
29680
23925
30438
26094
23888
23957
21627
4202



4162
4205
4199
4164
9176
2146
12300


415:
15090
9360
19558
17391
10057
17102
15552
29057
1555
15910
6334


416:
23286
11758
1274
8225
27981
25684
25757
16457
16452
1391
12214
23260



23289
1744
11105
11360
5199
25720
25688
14705
6709
6649
16261
22979



22977
16994
16270
2059
2009
16265
20642
23803
20644
15180
15175
4941



4914
3758
4703
4713
16785
10080
16509
27451
6582
12093
3831
1155



9438
17429
4505
9638
12510
11369
12332
11407
12298
12366
12270
11488



11530
12277
12432
12339
12269
17458
17460
19786
19822
21082
19790
19696



19761
19793
19758
19719
19935
19903
19827
19930
19868
21151
21148
21112



19763
21144
19928
3788
4668
4665
4699
4673
16783
4824
16782
6878



6991
6910
6932
6936
6940
5992
4628
3791
4740
4743
26710
23228



28484
14653
14703
17093
15617
15646
15668
15641
15642
15670
15639
22762



13093
20149
2079
4751
4775
4982
6024
6061
6868
16810
4978
6837



5982
6907
26794
25144
24355
5247
3727
3754
16757
3755
3784
3787



4632
4659
4662
4707
4746
4749
4830
4834
4879
4909
4907
4912



4944
4945
4980
5768
5792
5829
5862
5899
5895
5938
5942
5975



5986
6059
6091
6093
6099
6869
6897
6902
6942
6961
6964
6969



3748
3751
25626
18897
18900
11659
3110
1332
23097
27977
1190
15240



5284
5204
5307
5306
5310
5201
5331
5329
5234
5232
5226
5278



5236
5239
5277
10149
2306
12349
27947
19479
19484
25020
24994
14113



14253
14109
14251
14282
15069
14138
30160
14321
14278
19566
14326
14195



19682
14068
14161
29739
10181
17666
10210
17706
17711
17670
17681
23292



16447
13249
28736
13813
4269
8971
24172
10784
6621
6581
21799
28030



14647
5282
27990
28015
16419
5909
5945
6619
6614
14800
17524
28146



21825
21826
8124
8126
8159
8214
28025
28023
28020
8218
8196
30192



4794
15242
2511
14452
25359
13419
1836
2576
16666
1428
1423
19836



1910
7820
10218
20683
29045
6026
6025
16449
4481
14869
10745
11813



20059
22400
11648
7691
16414
8183
16455
16412
16460
13181
2994
2965



26527
16462
11863
7168
20675
16443
20678
967
17461
2509
11502
7375



17492
28280
6553
17455
2264
16409
16416
16418
16407
16444
4844
14779



20794
12379
7963
19990
15714
670
11705
25327
26672
28323
23256
17560



18542
22080
13354
13352
21460
21463
3023
16390
14532
29577
29581
29580



29552
29576
29541
8329
6616
6645
6642
16406
5279
20860
3939
8558



3973
4297
17449
19743
8597
25495
25466
23522
22778
809
27528
1300



23760
29578
25760
25761
20932
11894
20864
9396
3800
15568
9826
11866



20815
26007
7525
8113
8080
19979
20833
20882
19973
20991
20988
21040



20992
20962
20958
20922
20875
7061
20135
14531
6555
3548
3465
3461



21597
21821
7008
19716
7001
19725
13816
19729
19765
25693
21823
15215



4827
5081
20680
8119
12128
7002
5206
25110
5305
16506
4866
4872



4875
4869
1977
15074
15072
15105
23221
23227
18915
6585
4781
15238



28000
27811
27816
27832
27962
27900
27901
27903
27966
27969
27994
27907



27909
27835
27840
27842
27865
27870
27873
27877
27808
27809
27810
27999



27925
27926
27879
27930
28002
27935
27938
27998
27958
27959
9095
3804



22867
17450
483
482
417
4731


417:
23286
11758
1274
8225
27981
25684
25757
16457
16452
1391
12214
23260



23289
1744
11105
11360
5199
25720
25688
14705
6709
6649
16261
22979



22977
16994
16270
2059
2009
16265
20642
23803
20644
15180
15175
4941



4914
3758
4703
4713
16785
10080
16509
27451
6582
12093
3831
1155



9438
17429
4505
9638
12510
11369
12332
11407
12298
12366
12270
11488



11530
12277
12432
12339
12269
17458
17460
19786
19822
21082
19790
19696



19761
19793
19758
19719
19935
19903
19827
19930
19868
21151
21148
21112



19763
21144
19928
3788
4668
4665
4699
4673
16783
4824
16782
6878



6991
6910
6932
6936
6940
5992
4628
3791
4740
4743
26710
23228



28484
14653
14703
17093
15617
15646
15668
15641
15642
15670
15639
22762



13093
20149
2079
4751
4775
4982
6024
6061
6868
16810
4978
6837



5982
6907
26794
25144
24355
5247
3727
3754
16757
3755
3784
3787



4632
4659
4662
4707
4746
4749
4830
4834
4879
4909
4907
4912



4944
4945
4980
5768
5792
5829
5862
5899
5895
5938
5942
5975



5986
6059
6091
6093
6099
6869
6897
6902
6942
6961
6964
6969



3748
3751
25626
18897
18900
11659
3110
1332
23097
27977
1190
15240



5284
5204
5307
5306
5310
5201
5331
5329
5234
5232
5226
5278



5236
5239
5277
10149
2306
12349
27947
19479
19484
25020
24994
14113



14253
14109
14251
14282
15069
14138
30160
14321
14278
19566
14326
14195



19682
14068
14161
29739
10181
17666
10210
17706
17711
17670
17681
23292



16447
13249
28736
13813
4269
8971
24172
10784
6621
6581
21799
28030



14647
5282
27990
28015
16419
5909
5945
6619
6614
14800
17524
28146



21825
21826
8124
8126
8159
8214
28025
28023
28020
8218
8196
30192



4794
15242
14452
2511
25359
13419
1836
2576
16666
1428
1423
19836



1910
7820
10218
20683
29045
6026
6025
16449
4481
14869
10745
11813



20059
22400
11648
7691
16414
8183
16455
16412
16460
13181
2994
2965



26527
16462
11863
7168
20675
16443
20678
967
17461
2509
11502
7375



17492
28280
6553
17455
2264
16409
16416
16418
16407
16444
4844
14779



20794
12379
7963
19990
15714
670
11705
25327
26672
28323
23256
17560



18542
22080
13354
13352
21460
21463
3023
16390
14532
29577
29581
29580



29552
29576
29541
8329
6616
6645
6642
16406
5279
20860
3939
8558



3973
4297
17449
19743
8597
25495
25466
23522
22778
809
27528
1300



23760
29578
25760
25761
20932
11894
20864
9396
3800
15568
9826
11866



20815
26007
7525
8113
8080
19979
20833
20882
19973
20991
20988
21040



20992
20962
20958
20922
20875
7061
20135
14531
6555
3548
3465
3461



21597
21821
7008
19716
7001
19725
13816
19729
19765
25693
21823
15215



4827
5081
20680
8119
12128
7002
5206
25110
5305
16506
4866
4872



4875
4869
1977
15074
15072
15105
23221
23227
18915
6585
4781
15238



28000
27811
27816
27832
27962
27900
27901
27903
27966
27969
27994
27907



27909
27835
27840
27842
27865
27870
27873
27877
27808
27809
27810
27999



27925
27926
27879
27930
28002
27935
27938
27998
27958
27959
9095
3804



22867
17450
483
482
416
4731


418:
23286
11758
1274
8225
27981
25684
25757
16457
16452
1391
12214
23260



23289
1744
11105
11360
5199
25720
25688
14705
6709
6649
16261
22979



22977
16994
16270
2059
2009
16265
20642
23803
20644
15180
15175
4941



4914
3758
4703
4713
16785
15326
16509
6582
9011
8975
12093
3831



1155
17429
4505
9638
12510
11369
12332
11407
12298
12366
12270
11488



11530
12277
12432
12339
12269
17458
17460
19786
19822
21082
19790
19696



19761
19793
19758
19719
19935
19903
19827
19930
19868
21151
21148
21112



19763
21144
19928
3788
4665
4668
4699
4673
16783
4824
16782
6878



6991
6910
6932
6936
6940
5992
4628
3791
4740
4743
26710
23228



28484
17093
15617
15646
15668
15641
15642
22762
13093
20149
2079
4751



4775
4982
6024
6061
6868
16810
6837
5982
6907
26794
25144
24355



5247
3727
3754
16757
3784
3787
4632
4659
4662
4707
4746
4749



4830
4834
4879
4909
4907
4912
4944
4945
4980
5768
5792
5829



5862
5899
5938
5942
5975
5986
6059
6091
6093
6099
6869
6897



6902
6942
6961
6964
6969
3748
3751
25626
18897
18900
11659
3110



1332
23097
27977
1190
15240
5284
5204
5307
5306
5310
5201
5331



5329
5234
5232
5226
5278
5236
5239
5277
10149
2306
12349
27947



19479
19484
25020
24994
14113
14253
14109
14251
14282
15069
14138
30160



14321
14278
19566
14326
14195
19682
14068
14161
29739
10181
17666
10210



17706
17711
17670
17681
23292
16447
13249
28736
13813
4269
8971
24172



10784
6621
6581
21799
28030
27428
5282
27990
28015
16419
2481
2473



28172
6619
6614
14800
17524
28146
21825
21826
8124
8126
8159
8214



28025
28023
28020
8218
8196
30192
4794
15242
14452
2511
25359
13419



1836
22347
8504
29236
1910
7825
10218
20683
29045
6026
6025
16449



4481
4203
8291
16087
7549
23421
5273
5270
29657
25948
5275
6968



15722
21480
14769
12074
6737
3041
3986
11648
5607
7691
16414
8183



16455
16412
16460
13181
2994
2965
12670
27234
6265
14420
3760
17424



8749
12663
29710
9123
11863
7168
20675
16443
20678
967
17461
2509



12471
4719
17457
17421
17489
6553
17419
2745
26006
16409
16416
16418



23189
16407
16444
4844
14779
20115
23570
19989
15714
670
11705
26672



25327
28323
23256
17560
18542
22080
13354
13352
21463
21460
3023
16390



14532
29552
29541
29576
13203
8329
6616
6645
6642
16406
5279
20860



3939
8558
3973
4297
26020
4934
16268
6700
5817
7285
24569
30389



25857
27134
25760
25761
20932
11894
20864
9396
3800
15568
9826
11866



20815
26007
7525
8113
8076
19979
20992
19973
20991
20988
20833
21040



20882
20962
20958
20922
20875
7061
20135
14531
6555
3548
3465
3461



21597
21821
7008
19716
7001
19725
13816
19729
19765
25693
21823
15215



4827
5081
20680
8119
12128
7002
5206
25110
5305
16506
4866
4872



4875
4869
1977
15074
15072
15105
4732
13465
23221
23227
18915
6585



4781
15238
28000
27811
27816
27832
27962
27900
27901
27903
27966
27969



27994
27907
27909
27835
27840
27842
27865
27870
27873
27877
27808
27809



27810
27999
27925
27926
27879
27930
28002
27935
27938
27998
27958
27959



9095
3804
22436
16838
20688
4511
25167


419:
14198
870
17932
16069
13851
13461
12701
18143
19044
9845
28935
12911



9746
12378
26844
6279
3256
6492
1927
14725
5210
16911
19053
15107



10053
10052
17902
18728
14921
21008
27672
15861
22004
25826
27570
30385



24545
7602
11773
2106
25012
29882
5639
8632
19341
3676
13801
17969



18455
7576
29080
30010
25127
8356
9350
10154
5237
9923
12796
8187



29896
5402
643
24362
15888
14391
3369
3240
19906
23740
26063
12643



4304
13273
11196
13304
30067
3274
7355
14440
5431
27525
12373
26674



13025
10728
19975
19976
13989
13166
13167
28931
27390
2736
27297
485



28745
7015
29168
3670
5562
12710
21159
16719
17020
1225
28194
28348



22794
9021
15530
5874
9984
11413
1550
422
19877
19878
26951
17946



2877
17097
18956
15056
21779
18201
2127
713
5162
15588
18293
7479



10639
7629
27675
7385
23019
8438
20405
25592
15584
9862
19958


420:
1766
25623
2634
8567
8546
8030
8035
28060
8033
13487
12426
12425



27566
16382
13471
13472
27829
21056
7641
5010
7722
17728
13546
9391



8454
11625
8039
26982
2216
28645
19396
6428
12857
6029
8927
26241



28882
2058
17137
23307
23313
14163
30357
1758
1762
1765
22795
1007



12696
20720
20722
21606
21600
20724
9877
8100
8623
9032
26511
15887



17511
19373
11822
19998
25957
20063
20812
20816
20818
24166
23982
8056



30201
2731
26481
8640
22623
518
24795


421:
17859
1238
22054
28433
14522
12713
2265
30161
7453
4765
16339
3623



16249
30466
23897
24523
18199
12780
12018
8775
20575
9860
9828
17997



22323
12760
6777
25696
8048
7233
7159
10567
11570
5422
13420
743



20960
20777
13662
6471
6450
722
1999
22130
15016
24746
17989
21018



18525
16321
25778
29549
4744
4911
15147
16008
14299
4655
18309
20204



13162
27357
28445
29670
12438
2514
2700
25507
27408
21700
6258
15916



23219
8612
21584
7769
21090
21432
24336
2356
30137
4195
24837
12189



4171
20221
23536
26716
7664
11285
23505
6454
21901
13438
27127
6046



28726
23456
15186
1641
24978
18008
19271
13956
24528
17271
1119
18682



4177
14894
2489
29476
22053
1277
2172
26999
13676
22871
16889
8678



12883
23881
9761
12855
20677
3738
22399
20134
18998
18171
2684
15899



7332
29308
9846
23454
17170
1559
28849
2839
27226
11010
10112
1021



29279
19520
19722
26879
28081
15983
17146
15924
5208
2143
4634
16484



3406
25815
25097
16791
22937
9830
25112
1379
28559
28606
16292
16260



16208
16320
8188
28196
5518
5513
11332
7484
24098
25192
11722
7295



4070
3808
20017
21780
1418
3680
9910
7166
23057
22218
23084
9268



11075
26038
12485
7431
18804
6947
8806
3674
28046
5006
28297
7847



24808
19618
22706
21561
4782
24417
3843
24691
11449
9680
30005
10365



4403
14746
20774
4849
29201
28648
20671
18342
23463
29918
24863
14394



9080
25560
8423
7517
27591
30030
7571
29151
7460
3064
8500
8416



18919
15655
22879
22882
14872
2032
28860
21021
11112
2577
10386
7462



7356
9905
21034
29197
8891
6388
15308
29956
25999
24693
19760
18166



14979
10427
18403
23159
5100
26531
26168
9132
29747
26986
7010
6925



9803
26555
30057
7883
20362
13923
21199
17720
10376
13693
28462
5696



15243
4681
11036
28713
23116
22490
18697
15953
16090
8882
29422
26513



10652
10658
12898
14981
6819
823
17872
20813
2674
19462
20810
2655



17278
17299
29880
9890
17589
14931
19664
2181
15988
17595
11629
10661



7177
12706
6147
6518
8191
28422
15040
11675
3972
3697
28043
5019



689
19234
10006
7229
7695
3574
29895
19441
28177
28498
18428
15614



3789
15573
29663
7406
22093
3093
27495
16461
8817
19093
18724
25322



6607
14736
5953
21288
17280
10246
18588
18586
19908
19910
15765
26810



19134
17663
17697
3638
21052
16371
21054
2935
15495
20728
22097
813



5137
9972
18986
10167
27136
14751
15479
22618
405
27122
29583
15609



7933
3355
24226
15048
13579
1887
8939
8964
13794
9157
24816
21858



5885
22336
3805
3802
24169
2292
26734
26770
6657
13127
26043
12384



16093
3544
14897
2567
27765
27766
27677
15125
20964
23705
23066
24145



25943
24093
17443
7635
1827
22760
29245
18346
14261
16514
16482
16233



16542
18557
9646
13061
28991
23979
2413
30304
24393
6062
867
1186



8879
3327
2945
11059


422:
866
7071
17930
870
17932
7072
16069
13851
13381
13461
13464
12701



12702
8312
13470
8308
419
19755
18143
19044
9845
28935
27346
12911



12908
9746
6906
12378
12755
26844
6279
3256
29096
6492
6491
1927



1926
14725
14724
5210
5182
19053
16911
15107
10053
10052
17902
18728



15861
21008
27672
14921
25826
22004
27570
30385
24545
17631
11773
2106



29882
25012
8632
5639
19341
3676
8591
5630
13801
18455
17969
12282



7576
29080
10256
30010
25127
9350
10154
5237
8356
9923
12796
8187



29896
5402
643
678
24362
24361
15888
14391
15244
3240
19906
23741



25136
23740
26062
26063
25138
12643
4304
3209
13273
13304
11196
13277



13296
30067
3274
7355
1871
5431
5433
10593
12373
26674
13025
10728



19975
19976
19969
18860
13989
13166
13167
28931
28928
2736
27390
27297



26448
485
7015
28745
29168
3670
5562
12710
21159
25904
16719
28194



1225
28348
22794
9021
15530
5874
9984
1486
11413
1550
26951
26948



17946
17941
2877
2996
17097
17095
18956
818
15055
18335
21779
15056



24735
24579
18201
2127
25041
713
5162
5181
15588
15582
18293
7479



7474
10639
10640
7629
27675
7385
23019
23020
12990
8438
25592
20405



15584
15585
9862
19958


423:
15029
25307
9676
8311
26916
23959
11677
23081
2694
13387
5447
10573



11670
13978
11123
26839
27158
3619
28393
16399
14284
11420
30182
18878



10160
16007
26522
15577
10156
24813
10771
10742
26803
2343
2380
2382



1591
2460
2383
2353
2462
2053
27103
26865
22418
18140
8068
27942



26009
29347
26834
8774
15488
13383
15874
8921
12705
14118
14258
21153



24198
28012
5588
16861
10216
23576
3923
6699
3918
3926
3920
20836



22849
892
16183
27978
26550
9488
20021
16915
6824
30294
16762
8804



7419
23008
25744
29149
14295
30146
25107
16558
19890
14319
4639
20402



8947
5643
1147
11395
728
5293
4510
26624
12288
27309
15368
17961



15252
23928
24860
11838
29987
14792
3414
11679
19671
2183
14230
13870



13912
27989
7587
26711
14106
3481
14124
24519
26046
6594
30393
22818


424:
28787
28026
26562
27850
3993
21457
13373
22737
25673
336
13510
1153



27040
13055
17285


425:
14715
27077
7091
8238
15342
28311
28335
29231
9534
4494
13615
18789



27725
1230
10455
804
9254
10865
12623
18172
22589
640
26535
13575



8164
6881
25375
27163
27466
27305
29441
9966
628
26476
23339
23425



23698
14548
21310
12160
5898
30372
17110
2245
22497
22275
20136
29114



3433
19544
22006
16128
16917
10830
10600
18991
28811
1336
25859
8919



13541
13587
27186
4947
3845
11723
27086
3520
15911
14958
14920
13075



25073
24141
24384
8424
26449
23782
7578
24615
5515
12565
1052
20622



9144
9083
30107
24035
11692
4715
26578
10028
19100
15396
12004
2277



26427
22150
4990
17320
14898
4014
11890
10187
2273
19281
17315
1602



25789
12947
23230
20113
8781
3902
3335
8931
22254
23950
15881
16378



21068
18992
28385
24260
21185
10953
17209
4756
23880
12048
2515
6473



2545
26896
667
13227
19734
29717
17470
5067
17620
1371
29876
5086



29140
12846
11469
8066
16701
15123
17065
16699
19711
23152
28749
28891



897
20121
27569
5843
22595
22600
26841
4709
4712
12337
22378
22374



24033
2913
24701
14475
23715
26245
19991
7584
10643
15179
18396
7609



23792
13602
7586
11956
19021
26545
15237
29474
19066
22629
22597
15821



1174
1175
21504
21692
27022
27441
10473
24973
12482
20713
6929
10056



2246
19651
27645
10303
24352
4478
17176
3654
2540
29431
22009
15070



1261
25439
5968
7887
7727
12448
24300
15193
18608
30313
6505
613



22166
17182
15820
23530
721
24333
10326
5506
9091
1357
3649
24309



9988
19229
13908
24866
1512
2914
20798
20262
13907
13447
12842
13566



6367
21312
19637
6549
5840
25058
18097
18571
15246
11060
27827
25710



1091
21748
21747
3653
17000
12027
29642
30144
22522
15275
11882
9236



28312
15527
18156
27362
22428
24687
5611
15499
19435
16504
7875
20945



15534
24214
28466
28436
28715
21448
28469
22921
27957
10454
20564
1417



15157
29858
20076
22634
25089
27558
8378
16523
4289
7819
22253
28208



5308
18162
18114
14939
801
19072
7646
9780
29973
5857
5882
26190



14988
17199
25151
28781
28804
24016
11569
13634
8363
10492
22387
13363



17138
14970
25926


426:
30316
28341
20440
21403
28460
8764
5483
10239
21294
3625
20041
25161



16037
29671
15602
15738
13680
4919
6243
23006
3034
752
8677
14314



13422
1619
6262
6521
23305
17002
18373
1173
1149
11674
28125
22571



22569
1752
28123
21437
1073
1072
5748
2955
27548
8337
6584
6580



4722
6764
20583
706
8627
3362
14034
10533
10535
15608
16784
6496



9194
29947
16882
29432
11684
1245
25870
1652
28681
7191
27632
29345



3908
26214
18387
6769
6400
20414
26269
17674
16459
8262
15546
9173



12354
29426
15949
5362
24788
8630
26833
19714
1878
16103
26526
14486



10208
24594
20180
13753
3056
6467
20070
26719
13574
28110
11845
29110



12541
29814


427:
1205
1202
23043
18478
21335
24048
24042
12899
18378
18376
20455
6551



19940
19978
19981
2128
18413
18415
16198
16668
3185
15855
9352
14616



2960
2967
2966
4462
25905
11227
28186
984
13539
20758
20789
20750



20753
20749
28534
25229
27701
17690
22452
24103
19988
21788
25087
19912



21275
19982
19909
24881
24899
26679
18852
27639
12055
16381
1897
1894



1892
20060
7377
10185
27612
27664
3919
27641
10110
28531
28470
2758



4227
10669
24960
23679
11153
19321
30078
21051
17845
17557
1936
23577



27609
28429
27692
20186
20188
20182
20177
20222
20179
20174
20216
20218



27667
19872
26730
18303
18438
18412
22954
10396
9203
29169
12043
1965



24374
25660
17724
29924
16729
20884
2141
19907
22922
27089
2063
26239



19936
19119
21272
19915
19985
13259
19916
18368
18306
18331
18333
18338



17515
18481
17665
16401
13021
7850
570


428:
9514
18208
27699
24672
695
1581
17502
19870
19688
17787
10946
18811



24157
19726
17433
18211
27587
29573
574
576
11124
19880
5553
16146



13361
825
17239
5348
376
9975


429:
29272
16408
24394
21534
7305
25504
8552
18079
18052
19030
28248
16816



24060
21344
372
1466
29462
29423
29420
29457
5650
27496


430:
17468
17393
17389
17390
16815
13067
12931
11325
16679
8328
9249
3824



11932
29725
21677
28810
13568
16519
17018
13170
24756
8004
30147
28329



23718
13963
27073
2452
16177
27300
14029
30291
19914
14387
21586
30281



21049
14678
4088
11967


431:
6307
3233
20896
9887
26622
4960
4658
27322
28670
6575
7676
25783



1426
12035
27335
14401
16385
27296
16425
13199
19424
11348
10680


432:
14667
22888
17013
13915
2925
12977
12675
17605
26291
5000
11907
1511



27830
18555
7284
15187
10929
24838
8371
15946
8322
19594
4424
21192



12206
20285
16940
24995
16946
29622
25643
29160
2942
13884
17117
20604



22279
23664
10325
29846
15526
4557
7795
10008
23496
21495
11465
8089



23533
8128
22340
27131
22562
2632
20012
2852
2822
23026
16348
10038



20496
10775
21267
6404
6276
11703
3535
4554
5180
19347
854
22228



21030
18594
23540
18642
16640
7007
21219
12820
20795
28225
12596
23093



7052
10939
17912
4720
7729
1132
11961
16203
2290
21596
8725
12481



13527
24777
20996
18559
1078
16928
11870
24078
25941
13975
13410
16065



26929
28457
14649
24650
10198
29393
30143
20951
28853
3493
21315
18940



23811
12737
26959
25378
5760
24588
7309
27680
23118
28100
26837
1288



8754
16609
6974
19882
950
10136
13906
20299
2222
6219
4225
20616



10333
3582
13413
28193
22074
20491
25961
16797
25977
12162
26811
10251



21670
5907
8757
15621
15814
30089
18491
25493
25232
28527
9940
15033



6789
19362
26832
29018
16333
25608
18330
4363
15127
13991
24467
16650


433:
13586
26653
1211
21651
12022
7022
19757
29569
2997
3218
4850
5039



28662
23364
9454
21001
26643
2073
2485
5532
25152
2326
3026
17909



12902
6337
6395
25729
3630
17316
20626
25864
20141
8996
11264
18844



2439
12006
20392
19335
10528
18253
29753
29029
7141
12472
22829
22392



24916
25044
10577
25658
7656
23543
19748
15439
3460
16429
25244
11383



3178
9684
8024
13697
12969
1800
17370
15570
26846
15654
6727
13647



18740
2031
24059
15716
13114
22413
11257
29809
3525
26064
17001
11260



5474
9100
18705
25582
12968
13934
7045
16229
8102
25566
2539
14525



12334
29021
8155
27787
2430
28510
23504
2444
6502
19509
14681
12036



21356
22862
29732
7184
16331
25968
1291
1269
20206
10897
13715
23473



27212
27582
30392
7950
10490
8383
28561
28767
14041
7504
956
28731



8392
29317
15261
13079
18879
18877
29327
3215
11731
11640
3881
3877



3873
26505
18966
20107
24144
10349
17179
3512
3505
4812
20783
4742



10253
12927
23474
15809
11004
21372
22045
9573
5245
27101
12822
13675



13339
29874
4348
18265
25189
14287
13515
5969
24595
21046
2836
29639



19902
18322
16979
22451
22391
10157
3223
17108
13403
7951
28632
3890



10280
13736
26628
26963
14499
27812
15935
5122


434:
14858
8871
2388
29193
23611
29343
15969
3408
5093
26787
9002
6519



9143
17653
27755
29466
6232
3608
11358
11361
1254
15971
25586
30150



28671
30162
1856
6784
8680
6054
12435
25468
24267
6090
17810
29384



25428
24820
7303
19932
5951
11255
18631
21360
2946
21362
4654
28107



6605
19931
23748
28575
13516
13519
1247
7513
12271
5985
5453
9951



23296
25872
28950
20096
26412
12419
22978
27642
23608
28276
674
11249



23350
11976
12001
14156
28285
29661
8658
25191
6043
7432
2118
3482



11935
11939
28718
26871


435:
15751
12169
15239
15929
26189
15900
23906
16887
12540
20889
14441
21704



21676
13545
13492
27980
15688
23036
4892
12295
20500
3991
3945
29238



2237
29105
28374
17908
14292
15115
15732
10955
23971
7986
22459
17962



20020
22328
13357
3594
16689
22346
21444
18354
16734
6074
6816
6620



8267
8178
5244
25917
29414
24598
824
24242
11712
25712
24715
22599



14137
1053
4244
19514
2978
10289
29087
28834
9109
18405
23656
2850



20632
20853
14621
7225
16586
4231
28270
2784
20857
21716
16695
28188



14495
24469
16671
17896
25055
1375
21390
16442
8600
17234
15699
18742



26263
26398
28320
29127
26400
21029
9971
21645
27577
26419
7025
10796



11992
4326
10214
2221
24451
9329
15797
13644
16721
15943
21150
27748



9850
12756
26739
9013
18025
9579
3723
2525
7944
21545
27260
762



10551
11851
25402
5816
5959
5963
11441
7858
14233
9186
19677
2108



20171
25345
1008
28748
28073
25902
6516
5265
16835
7259
27489
27484



26410
2544
1668
9478
28876
9416
8991
15898
15807
30362
18777
19596



10802
27691
17911
29983
1252
29903
15359
17051
24096
10904
4052
19449



28227
24046
30332
20549
5103
12103
18923
22957
9111
14917
24695
18894



1680
19563
19709
28772
17862
5036
6133
19859
18950
25894
22091
16880



23175
6946
24953
26197
16245
30312
1742
21807
3593
18707
10847
29922



17586
22427
3131
17609
4243
21802
13346
17538
27169
13247
21393
10263



6930
11342
14500
17328
14503
17326
19129
8860
23051
23053
19595
6899



14063
9994
16599
8079
9386
3626
6011
1901
10005
2508
3833
2727



22316
22439
25992
23112
4523
3865
17168
28152
16258
30052
14089
23833



966
5414
13058
16496
30350
15440
6125
21778
25751
7583
4067
14007



23808
3291
6813


436:
28743
16580
16834
8593
9933
3230
27502
12572
12452
7671
30301
30190



20166
10222
5692
9903
12874
30037
20309
11529
20004
21371
26141
22217



21455
12017
3239
4147
3374
29961
10409
15819
22560
909
24291
27198



28843
9248
15121
14344
5035
21019
15096
28579
7985
7631
20586
6973



10171
7070
26342
24979
612
11600
1087
28446
22111
10434
27911
1686



10892
15085
12829
16500
7633
10962
6095
27281
24640
13480
6844
7181


437:
27723
4530
4561
5996
6561
27265
3876
5881
17755
18096
8944
19900



18971
18960
18994
2864
2862
5061
17682
14938
14940
14944
26943
26861



19086
4597
3083
8858
14023
19466
8886
17367
29465
7832
19071
9894



28471
20261
15954
22612
22610
22653


438:
16231
8527
24427
16494
23245
23271
12322
14082
12321
23243
6257
16309



9449
4003
11697
11141
1172
18278
8801
28324
13460
19497
18891
21306



10064
3661
29176
12129
28789
7632
22164
30327
5170
2321
23701
19543



30045
9256
2894


439:
10346
18603
23885
13652
9175
18536
13700
6245
22789
19622
14498
24408



22191
14417
22910
23910
2072
16138
14112
25002
22454
25878
8444
25302



21271
4988
25900
1601
24221
27057
9216
10965
3383
26732
27190
3116



11200
27744
13335
18970
12918
3012
15494
22942
30223
22293
28246
28244



1265
17327
1421
10302
14910
6065
12687
17866
17957
22396
27972
5764



4238
6979
4468
10259
23150
5405
28344
19453
3581
24255
8934
30181



3198
22810
9827
23415
4925
8386
20191
1777
29750
29952
5195
25155



27222
25791


440:
12773
10744
27007
24376
14280
27625
17836
23138
27078
14316
12218
25743



18082
4788
6886


441:
4509
26259
29760
23472
11198
27076
3262
6507
15178
16996
21932
12251



8372
24476
19290
5080
15711
29081
25694
29597
3354
23050
16758
29935



6107
2900
15219
1960
24898
28176
22447
5585
26079
16049
22377
29574



24681
29289
11294
14961
22642
24789
15211
18091
22605


442:
8367
6864
12524
20258
2547
30153
14333
6312
5797
14892
12811
3222



4007
28352
15723
8465
19840
20803
24946
3331
22830
4472
8957
22970



26158
3251
9292
10270
2480
1321
18185
16968
6298
19777
4959
5834



18110
7965
14822
18240
19154
13821
16977
4206
28210
15355
9517
16060



26755
27815
6034
23493
4200
826
25552
4105
27433
28392
13728
18554



13734
30455
30452
13731
20476
20411
21515
27155
9956
15002
6480
6362



20227
26521
28901
24447
24986
13091
13072
17565
14590
23862
12052
2002



9380
1899
11464
17311
27666
3502
3880
30334
29240
8511
7060
18584



2755
17985
10141
11821
12465
10738
22430
6254
24904
2639
28985
29136



25807
22711
3439
10513
1250
23823
26967
4299
26218
30125
22784
15188



6648
5359
12680
12088
26702
26701
26639
4221
2244
3899
22988
28951



23620
27540
21636
27324
13874
6489
24288
3333
29400
9364
6638
6798



5535
21283
8864
11272
15682
9557
8827
6223
29321
15771
25975
11631



21357
23503
16608
25936
23575
5709
8692
9082
8690
11913
2726
4264



17585
2351
676
23242
12174
17019
6360
10976
22461
4228
17644
17751



18677
5490
10327
28657
22256
8143
23747
1904
29771
24665
8223
25399



15704
27065
21382
2348
29390
12948
5682
22699
10408
19222
24698
24885



14398
8544
21707
8901
18093
11520
21854
15853
8974
8391
23652
6637



9047
28062
5315
23486
26636
28105
25555
26657
18509
11091
7075
17996



1791
3859
22197
2691
15475
17854
16639
30356
18798
18770
18765
11805



3174
11751
11406
684
12736
18606
10095
19895
18781
5482
4410
20156



27217
9641
25562
29866
5145
4241
18691
10393
28993


443:
15900
15751
15929
26189
16887
20889
7980
13017
24338
24315
12585
13524



23036
12295
3991
2237
29105
17908
11425
8460
8123
9031
20801
21161



4787
5900
22459
17962
20020
13357
3594
21444
16734
6816
6620
5244



8267
8178
29414
24598
6965
4922
29122
28155
1053
14137
18085
29087



14621
2784
16695
20853
1260
17896
25055
16442
18742
15699
26263
9971



26417
11992
26419
24451
15797
9850
16721
27748
21150
9579
18025
2525



3723
7944
21545
27260
762
10551
11851
11441
6398
9186
2108
5265



27489
9478
15898
15807
30362
18777
19596
27691
1252
15359
17051
4839



4842
24695
1680
19563
2957
2962
27985
16880
6946
24953
26197
16245



20502
20499
20470
30312
27948
12885
3379
6810
5540
20456
18124
5060



18003
13201
7969
4342
24884
30103
30076
30082
23051
23053
19595
14966



27988
5426
9422
8079
29927
25173
4523
27982
25080
26093
23156
17194



25140
24032
11230
18381
23833
19925
26095
3237
28206
12469
7251
7059



3629
25751
21778
6446
16561
16775
3291
10423
8415
8365
16557
11016


444:
703
16986
10530
24696
29631
15216
19465
12205
2111
13009
733
3123



5782
12717
22838
3301
7207
3177
1650
10162
12118
18359
22793
12486



27776
663
5452
9594
21011
29500
24292
10637
19035
23055
9212
25016



7139
24727
26715
26740
17536
11580
16466
28242
9045
14101
16469
13665



26392
7824
27393
10521
20413
15589
19636
23467
5187
5184
27095
19483



25993
3806
20544
4580
3739
23095
6653
9945
27764
6115
18456
13215



14893
4321
16612
11020
25876
24618
20971
30299
27399
24167
24220
27674



23909
29116
2454
22725
10075
6722
27143


445:
30054
9562
9529
9525
9566
9603
23232
9751
11028
6117
14648
4395



22884
24631
21491
21653
4029
13588
30436
17233
11596
11194
9508
8431



4484
12640
25503
18864
21979
11634
22280
6114
27302
3729
19968
20580



22038
18324
18326
761
9516
15020
14996
17577
27784
19686
20530
29949



13558
13626
20254
26352
20100
22776
21270
2907
19221
25572
29976
28066



28112
28114
25506
25483
8868
13129
1944
25485
9569
7868
21107
4955



14457
15310
17238
7103
21488
21487
7104
7108
14471
15941


446:
24743
18890
20338
15579
24945
3403
11390
7926
3786


447:
13827
24653
2760
21851
26157
25150
12111
2562
20140
26647
15801
2390



3532
9193
23744
25253
21177
3080
11263
452
13134
23306
9258
22014



15200
15385
14210
2432
1137
20189
7975
21708
8011
12963
29490
18633



28799
1182
5174
25735
25459
5789
30130
15317


448:
2789
15523
18289
30198
24495
6772
654
15067
13394
28473
7794
12180



5835
28884
3735
14624
10296
15431
21816
2139
13185


449:
20866
29212
14854
9418
29467
18050
11000
15581
6435
25384
19947
27875



18498
13045


450:
26090
24906
7328
16079
13640
1998
3987
14369
24547
10118
10168
19534



14201
4351
29751
23972
19048
19124
19518
16929
12836
10980
5385
12514



8603
21615
14006
11639
9621
29755
3278
9131
13564
783
18177
14122



16367
23248
18197
16067
10484
27976
27790
10726
28360
7845
8361
7214



7541
3978
10445
26641
12573
11417
1937
8810
18305
17353
26604
11764



14422
27199
26491
13341
8502
7149
14121
22354
27631
850
24218
22409



8455
30360
3313
24294
14091
12152
30220
23301
604
12792
28356
12273



17439
5642
24682
2394
781
21304
19627
7391
21743
5612
2420
8426



27392
19043
22515
29067
5266
8170
5679
6456
11093
8388
22771
8450



17154
17655
21229
10695
22850
20714
3703
17981
27996
24666
29419
13898



25251
2927
968
25866
11823
11437
11556
29559
28036
22558
28576
18030



644
20487
13123
6229
21755
4019
11073
14826
8138
4868
5476
13751



26764
24215
21989
8521
23457
22084
15037
29764
12464
14383
27005
11776



28824
9025
18016
15103
18931
28602
8158
17472
1453
3416
1990
16594



2573
20483
22565
23359
4684
14747
21853
24807
9107
9108
22028
22025



22021
24031
21991
26569
29011
5830
3328
15130
15099
21719
26251
26252



13720
2076
22740
24231
19986
21220
18601
2781
11918
4664
8250
28676



18541
28331
28337
339
4767
28342
28339
5219
26295
4537
1807
15313



11247
25153
12268
2308
4168
2771
29692
26107
29685
28833
9328
2456



2455
2459
27366
27372
27370
13267
13269
9730
9757
9762
1804
588



10917
27732
21183
2693
16543
21668
16222
29162
22592
7464
26199
15576



6058
2248
16943
6613
18400
6406
28875
30169
21426
14657
11572
11571



10874
15508
22276
22496
21391
21149
20099
21996
21999
14338
14342
14140



14363
14341
15131
6669
995
21986
5549
14372
14890
14882
14886
14908



30442
10627
13868
14633
10628
10603
9996
18761
17083
26475
9727
16415



17048
28330
24234
25184
7849
29233
12077
18458
16866
6253
15009
21468



22604
21893
16765
21349
10996
2998
16618
22757
11069
29828
10308
16323



20432
26608
11347
18818
2720
22764
341
3311
28703
1361
548
8249



30246
14879
25683
1195
26838
21940
16856
12346
24829
21523
8831
29982



18688
11159
10760
21202
8264
28303
22043
25091
21992
8269
28346
13382



24297
24316
13140
2970
22223
16540
763
16890
3910
16235
4535
4961



11545
28378
28077
2792
23797
2309
14104
7723
27167
10108
12589
10780



13561
10432
24018
30465
23985
23865
12964
15841
26552
12280


451:
26090
24906
7328
16079
13640
1998
3987
14369
24547
10118
10168
19534



14201
4351
29751
23972
19048
19124
19518
16929
12836
10980
5385
12514



8603
21615
14006
11639
9621
29755
3278
9131
13564
783
18177
14122



16367
23248
18197
16067
10484
27976
27790
10726
28360
7845
8361
7214



7541
3978
10445
26641
12573
11417
1937
8810
18305
17353
26604
11764



14422
27199
26491
13341
8502
7149
14121
22354
27631
850
24218
22409



8455
30360
3313
24294
14091
12152
30220
23301
604
12792
28356
12273



17439
5642
24682
2394
781
21304
19627
7391
21743
5612
2420
8426



27392
19043
22515
29067
5266
8170
5679
6456
11093
8388
22771
8450



17154
17655
21229
10695
22850
20714
3703
17981
27996
24666
29419
13898



25251
2927
968
25866
11823
11437
11556
29559
28036
22558
28576
18030



644
20487
13123
6229
21755
4019
11073
14826
8138
4868
5476
13751



26764
24215
21989
8521
23457
22084
15037
29764
12464
14383
27005
11776



28824
9025
18016
15103
18931
28602
8158
17472
1453
3416
1990
16594



2573
20483
22565
23359
4684
14747
21853
24807
9107
9108
22028
22025



22021
24031
21991
26569
29011
5830
3328
15130
15099
21719
26251
26252



13720
2076
22740
24231
19986
21220
18601
2781
11918
4664
8250
28676



18541
28331
28337
339
4767
28342
28339
5219
26295
4537
1807
15313



11247
25153
12268
2308
4168
2771
29692
26107
29685
28833
9328
2456



2455
2459
27366
27372
27370
13267
13269
9730
9757
9762
1804
588



10917
27732
21183
2693
16543
21668
16222
29162
22592
7464
26199
15576



6058
2248
16943
6613
18400
6406
28875
30169
21426
14657
11572
11571



10874
15508
22276
22496
21391
21149
20099
21996
21999
14338
14342
14140



14363
14341
15131
6669
995
21986
5549
14372
14890
14882
14886
14908



30442
10627
13868
14633
10628
10603
9996
18761
17083
26475
9727
16415



17048
28330
24234
25184
7849
29233
12077
18458
16866
6253
15009
21468



22604
21893
16765
21349
10996
2998
16618
22757
11069
29828
10308
16323



20432
26608
11347
18818
2720
22764
341
3311
28703
1361
548
8249



30246
14879
25683
1195
26838
21940
16856
12346
24829
21523
8831
29982



18688
11159
10760
21202
8264
28303
22043
25091
21992
8269
28346
13382



24297
24316
13140
2970
22223
16540
763
16890
3910
16235
4535
4961



11545
28378
28077
2792
23797
2309
14104
7723
27167
10108
12589
10780



13561
10432
24018
30465
23985
23865
12964
15841
26552
12280


452:
13827
24794
24653
2760
21851
26157
12111
25150
2562
16927
20140
2390



15801
26647
11245
3532
9193
23744
25253
21177
3080
11263
15317
447



21560
23306
9258
17954
22014
15200
25970
12414
8034
19378
21298
15385



15384
14210
26968
8346
2432
1137
20189
7975
25141
8011
18633
28799



12963
29490
1182
5174
25735
22547
13204
6963
18646
25459
5789
23252



30130


453:
22309
16508


454:
20066
8631
6983
6988
7348
17302
7353
2347
9889
23658
24193
16953



19830
25103
21473
26882
28832
22567
14661
23064
4384
2368
20595
27093



5979
23062
6031
14031
21060
15345
17493
13555
16552
6038
22502
19406



11005
29928
13879
6299
9551
30298
12283
13468


455:
12197
5083
22748
25233
21145
4018
2633
16102
12779
29833
11193
8821



8822
5591
5978
7241
27069
19493
24507
10625
6544
21805
23685
23663



27510
27513
27515
25379
29072
846
1262
16659
23446
23314
2834
25292



22709
8169
20003
21182
18200
2067
28820
18356
27543
19791
7792
12744



4194
18884
2842
29496
8027
18247
19024
4805
8531
18422
20244
20210



19630
27242
25821
6050
20022
17852
21434
19090


456:
5713
5731
7666
23694
22911
18150
26649
2034
8001
30006
10177
4692



14395
29033
29544
15443
10544
24797
10873
7935
29003
15255
4236
3050



13865
21949
14356
1329
28334
11607
21180
26293
21077
16585
5701
3709



27462
13332
14583
25043


457:
21969
407
6567
2642
681
24237
27172
24706
5892
8105
22041
16218



2316
4924
22112
26146
19293
8274
19356
14025
379
10900
18809
3477



753
18266
26407
25408
380
21926
9209
19607
25045
14021


458:
16744
4407
25100
21084
25750
22690
20467
9787
22694
20474
19088
14913



14914
24851
21000
1213
16158
30234
4889
30041
30040
18311
6589
2008



2276
29636
10560
16082
12880
12882
27184
3985
10180
12256
15752
3953



22588
2670
5009
17129
8599
3657
11624
14128
727
11869
18500
14209



19819
22815
20307
17323
25048
18179
1414
24660
24638
22963
17432
26842



22880
2592
19847
20451
3055
10336
2910
22753
22754
13718
24605
2358



4981
1851
12081
25037
17222
17221
19195
21936
16573
7414
9623
23310



5178
29027
16016
25544
10442
21658
21659
28729
7026
26969
29301
26753



12164
18602
1243
29091
3701
15406
8344
28267
6135
3561
10671
15878



10750
10970
6970
23035
8118
17491
5018
12726
12942
14767
29257
27629



25172
25170
7283
12727
24619
27552
16255
29256
28279
25594
876
27774



17636
30055
4952
6912
6914
4950
26688
3371
3356
27012
15203
12615



21483
11271
20441
29061
23791
1564
16732
8867
6163
28544
26455
17744



12012
2253
26349
4803
4804
27446
17956
17955
17091
26563
25681
5047



2835
16537
6565
7807
7176
6483
18151
3560
28942
27493
668
14152



8834
29598
29340
6273
4878
1909
11746
11745
14903
26071
26700
13493



27961
11683
21235
28009
11760
13876
17809
12752
10315
8005
12860
16240



24121
19108
14075
27793
1494
30050
22237
732
6109
27507
16471
15948



13484
9708
10477
19339
22137
18287
14289
25145
14303
22047
19644
2391



23284
26180
1201
17522
7880
15095
23874
16577
2294
20130
14936
14915



8994
25135
21336
29920
10839
11346
2350
14108
13633
15851
24552
27770



27149
13108
25406
30425
27387
19343
18819
7131
24769
19476
21522
17365



12929
12621
7081
19263
14881
9813
7973
9239
25883
11769
28879
27072


459:
1392
27440
952
11231
20435
16441
9264
8684
20316
3765
21565
22449



14297
27098
21009
26075
13940
15745
18825
24720
3267
20672
20701
3772



29912
5984
21895
18957
22067
1830
18410
17473
3764
21358
24490
16843



3480
8815
4317
10147
28888
21736
16935
17136
10010
13924
3604
23429



14793
20512
13262
12013
12007
3937
11715
9034
24957
20209
23672
21633



29718
18153
10301
1298
30398
1600
17050
23327
25040
4042
3785
6154



9882
4288
17796
14231
23134
15162
22444
19275
11523
11779
9051
2667



4649
4648
1499
20517
19837
12567
16221
6806
6472
773
27635
11542



6829
29280
22140
11949
11952


460:
23201
17383
23224
26008
26005
9941
5941
17348
25254
25230
4414
5687



10674
1801
2767
19184
19713
8809
601
25137
10779
26745
24818
8897



1368
27623
30093
19413
28690
18037


461:
2488
2490
2492
16114
16109
1842
2374
7721
16119
1863
25039
2379



2376
16118
1861
2372
1843
1868
16155
16157
16159
2457
2461
28195



28216
24571
24556
11512
9497
9502
9278
21661
10042
13872
1959
5069



30433
3795
24649
23526
17615
13518
29873
26193
6169
7274
4943
9750



17943
27097
11513
11965
11516
18895
12284
10013
10941
615
26596
24685



3883
16089
27100
5943
21917
20253
13576
7539
20582
698
16187
2401



2400
4092
16152
2337
2340
1866
16149
2342
2345
11604
19568
22680



2889
21674


462:
28452
28384
7106
11134
20318
6599
18866
24932
26345
16954
25858
26047



6501
8305
13871
28850
25122
24370
8396
30331
28333
25796
6640
12891



17285
6631
9286
5090
29297
1218
18883
1344
5464


463:
26045
30127
29320
26289
12924
11235
5935
10527
17821
630
29473
12869



11451
5618
10027
14888
11990
24259
5720
780
9122
27531
18352
14061



14010
13206
17734
13867
14560
17261
17144
11790
29913
9244
11170
24312



4819
23638
12648
26815
19921
17384
7194
1019
1017
25228
25224
17767



29602
5818
13327
1950
21814
6787
25734
26586
14652
25785
27175
14530



30381
7252
14959
30282
4979
23724
6001
11024
7290


464:
15367
24282
29815
8366
819
4826
9906
8903
15845
24726
12243
13433



2043
20438
18051
3857
2660
8084
15539
17178
14718
2092
3558
9493



24848
7892
15086
25526
23930
30058


465:
20805
12922
14821
25099
16808
23561
25115
19924
19858
30036
10807
17562



17559
13375
13378
19173
19143
19138
19170
13380
19109
16611
28475
16515



11072
13792
4888
7308
7306
7339
7337
6325
6321
7239
9620
5776



18454
8853
18492
13237
6359
27246
5222
17478
16250
14573
14576
14602



14599
14607
14614
14611
16303
7130
3624
2938
24066
1364
10344
8055



11763
12901
26566
22412
19923
4023
25159
4893
3213
23466
27085
14216



14507
25168
8796
5832
8091
27991
26781
17266
10209
1347
7032
27523



19861
19863
19926
25579
15446
30369
2973
18544
24922
21575
30296
11175



11752
28465
6888
11296
18715
27288
16176
29939
2236
27166
20256
1533



29617
8182
4467
1441
28304
1107
10964
10352
19316
15371
25916
17254



13035
15081
12315
25001
26950
25641
18276
3069
21746
27359
13083
25323



8988
17953
705
17263
29051
25622
30273
21798
9886
18577
29842
29346



9571
16738
8098
13661
23941
28640
8842
5528
1458
15064
2712
8598



12718
22843
3848
19776
19901
19899
25119
18720
14060
21819
1461
24111



8925
19891
19894
16276
1446
6854
21398
24699
24222
23460
20936
4995



14002
26439
19176
17864
9545
7929
4591
14774
25731
17634
7345
7350



26023
26027
15925
9411
14829
10453
29997
14801
5479
5477
14857
25218



9564
12476
20797
5125
6667
28570
22388
16525
25779
12682
17030
26024



25988
26019
17486
25989
25990
26021
26056
25986
18092
13750
1705
30145



9421
28825
13043
10654
25972
13240
23075
27963
12518
2179
29108
27888



17849
17820
17851
17816
17844
11955
17848
17814
4789
15859
4784
4753



4783
4785
28898
11378
9600
13882
15012
14989
14987
15013
14983
10193



9453
9409
8209
23604
15400
10184
18398
10206
17942
13948
26377
6187



6664
6574
10958


466:
9833
10948
27731
11438
8716
8587
20505
29173
12404
17922
9506
8836



26632
12107
26223
1941


467:
3744
2450
26907
24268
6387
21543
27201
3273
475
25458
8487
4208



17673
26773
23524
5099
1386
5956
3486
23059
30021
20480
18160
18918



18033
25273


468:
29430
14714
28230
1782
14011
27396
5339


469:
16873
25672
15458
2920
2728
1220
8483
17024
20028
11789
14581
6962



14566
6780
10497
18752
15596
7590
14877
24206
3031
9852
8813
25593



2133
21408
5993
7816
1485
23125
17967
19580
11617
29950
1964
13535



7873
1700
25906
27047
3109
21870
22860
3951
13040
16584
14426
18304



13505
14575
3047
26072
13607
6818
2339
15046
13231
11155
8634
21648



19697
14033
3695
19659
7790
10350
30202
30173
15759
21307
16088
15050



29904
4276
14093
11685
9214
857
10093
8047
11053
1895
4951
9337



25914
11817
11203
24772
24834
20533
6853
14954
19965
25009
20267
7919



6720
28669
10416
4564
4548
22203
22633
22630
14275
15521
9359
14354



5860
11606
5856
12897
999
24729
12418
27660
5646
4764
18470
30417



1189
1688
9661
30170
24583
24527
20152
8115
16262
25419
26123
15518



22544
16437
12809
6172
10896
29571
5756
15816
10947
13903
25047
12762



1419
8259
18553
16718
29881
12446
14386
18195
9265
15463
14948
19151



26404
26568
5361
8368
26541
8689
5994
10932
16196
7218
22411
7126



4340
16226
15830
17244
18927
11252
4651
1527
29455
29211
1718
29888



23402
27203
1140
17025
29313
25203
27384
11267
14067
15004
18433
28130



21635
1457
19207
10986
14070
13770
19606
27889
29378
29163
30302
17445



3879
6439
5119
25676
2554
9067
6426
3320
858
19522
23558
25517



25543
3398
17672
17588
18395
18393
17554
17622
17659
17551
28516
8929



11986
20844
28512
3782
24943
10076
1249
30461
5451
25647
16875
4259



1156
9135
15348
29495
21381
30038
30133
26467
2180
3897
8197
9243



12254
23856
3634
10356
13042
10165
5990
11909
22525
5883
12983
24350



19164
4224
7398
6610
7726
29557
20165
1257
1221
22579
9510
6560



27739
10111
20005
11991
9437
16683
9431
701
7408
18332
19698
19474



22934
23815
24314
19083
24047
13962
16440
17202
17175
16541
9468
27887



988
29910
22209
13074
4047
6656
13329
22189
26333
13077
5151
8562



11947
12765
13008
1045
29454
21234
27837
25093
22281
5399
24382
25352



23626
16851
3427
7600
3518
2777
10977
23551
15202
27262
18511
20454



26958
2990
25620
8561
17718
17761
8331
29962
7603
8538
25241
18032



18042
2329
19041
11662
29586
28661
18523
18497
18056
7497
758
6311



3195
16630
15553
21809
28814
11392
28345
17873
22891
3413
5446
21187



21059
17392
13230
14310
25442
17453
29069
6194
18181
24627
24597
12030



27412
10536
6692
22997
5101
29651
16078
21617
14348
9403
25722
25013



24988
6236
28169
29641
4623
25258
26524
8968
14651
18839
5130
5690



24709
10506
11668
19887
14443
18623
19994
9066
11064
28970
10838
27657



29353
8576
4060
12604
9332
14088
15142
23625
13684
29167
14208
17255



16837
23853
22395
22373
8621
1194
16942
20696
23619
9410
17440
22288



24562
15990
2435
27045
19807
21524
25176
25597
26483
19055
26599
29954



1733
3453
8942
19139
25262
28205
13959
2466
23563
23535
23938
1106



26394
27432
15335
23743
30073
25540
25509
788
16908
22285
4058
23356



16019
9448
13342
13320
4398
5838
808
16813
11977
30287
2318
29144



25946
26119
29817
21184
22289
18170
4310
5324
17550
1349
12618
11468



11595
8061
15325
9505
22350
16335
20449
2163
852
2733
18301
17686



13850
12974
4897
5048
15813
10685
15928
12455
29570
20665
15052
26244


470:
12633
22272
6939
5436
6903
19289
12073
4246
11239
11220
5207
17109



24162
21590
19286
5425
5430
28756
5610
28547
30375
21508
15517
23879



24168
15269
22766
30444
5774
2159
22995
14625
22961
27546
16625
25748



3774
23778
18896
29115
22848
6662
15338
18141
14813
4311
12188
4186



17651
16654
22596
24543
24139
24171
13070
14145
15047
9867
22339
6261



7342
29017
8122
25903
12863
29221
7811
21016
20259
12960
24163
23831



24134
3722
25962
3343
17871
18224
24138
13856
9840
17246
18426
5864



29945
28973
9848
5133
10174
26116
16948
21378
855
19993
30218
11003



8090
28428
12644
14159
4657
7703
7704
24137
16308
14049
13022
13027



12177
2556
7496
13569
884
18847
16724
13023
2419
28613
14144
3276



26725
23143
23690
23691
10724
2410
20847
6017
26851
16062
19848
10284



16302
7117
15902
16224
27646
13245
16310
5202
15607
19751
10030
16301



16282
5225
7893
7921
7923
1983
17040
12399
12402
12401
12397
12398



9089
9591
11778
30113
13024
16120
6482
13019
13004
26633
6479
26162



20447
21352
23855
993
14542
2101
992
13020
5205
6181
11536
13905



14825
24913
24331
16312
9484
20036
20039
14812
20073
1039
2389
2274



16607
29034
16307
21350
29213
7738
9401
30222
7652
19703
24790
21351



1082
3958
5534
20602
16304
4223
29001
16931
24164
9476
19512
19513



5233
8163
28865
27974
13483
9874
13645
27614
15344
28778
10055
10085



908
23139
26856
14272
27905
28728
13538
5203
5175
9222


471:
22195
30152
6215
18310
30176
15008
9053
20909
11090
13937
29492
23498



9540
22473
7063
15879
22002
1784
26042
21862
22104
20425
18226
17435



17496
6569
19294
13823
4369
21849
9672
23557
13834
9321
5013
17783



23639
30253
21258
18100
18203
23366
1867
29161
7090
8065
5912
21906



27553
20415
22173
9336
3393
597
2428
19220
28235
23451
8008
20477



8963
4593
10304
28707
16373
1451
1649
24524
23177
2929
27941
19444



18785
19253
16493
19416
20332
6876
13131
10202
8699
24504
10090
15533



7826
16346
22825
3442
12372
6892
7068
29938
13101
29032
15744
24108



24491
8940
20067
7670
16960
6558
20509
5537
30330
16325
11892
900



24379
18788
18563
29104
4890
9327
8618
3255
16033
25747
3633
17301



28400
8097
24192
2272
5220
7448
24170
6248
1783
10060
22564
5407



16030
6997
2224
12460
21439
23198
2414
18351
30061
3603
21954
26188



2015
4725
18641
6042
26953
16528
1292
23424
30419
915
14133
16667



13532
9233
23431
14659
29349
13674
998
3220
29610
22704
21923
27232



16230
17364
14469
28827
26985
7838
20249
13966
13272
7911
4800
22133



26192
24647
27313
13818
25557
13638
17495
15507
26227
19646
24766
8950



16865
27760
28258
26805
25529
25465
22945
6063
6587
28808
6247
16538



6524
12656
2663
25855
21840
29993
27653
21451
28266
8983
1299
10274



4886
11987
3436
18214
29516
7231
24843
19829
21657
20943
2763
28674



29403
8032
20989
7748
19920
4818
1439
13349
10552
13489
26254
19708



15937
19611
18759
30448
2504
1855
14304
16161
14794
27687
918
22692



20085
5459
10458
18793
7582
4992
7411
12999
20450
1903
23661
23637



9363
26777
4504
9414
19764
12316
24423
20929
26737
6860
29546
9742



8220
4128
28979
28018
17279
19419
8050
6749
26314
23455
21876
22644



20424
23606
8564
23299
23267
23362
23336
10269
22675
23589
26780
23610



26749
26752
19407
26778
16533
18291
3340
22005
20418
29511
16025
26731



28962
20199
29688
8236
27950
27465
11943
11493
28696
9754
13165
25873



28209
835
24845
3447
5559
10898
9504
7751
5357
26525
29818
13252



13804
25753
16100
4034
12595
24263
14146
18949
7005
7898
25294
6499


472:
27556
7298
2699
2702
21764
26703
28272
933
25223
20176
2115
8738



7560
22098
12859
26875
27530
30084
12326


473:
1966
1962
19645
2659
22951
7955
17023
3046
15490
23268
7909
10931



9358
24015
7511
24796
28896
3334
20294
29399
9729
1315
12080
12690



18145
6120
14300
5798
24062
15082
18474
10392
20038
28782
27211
27210



16534
23675
25578
25574
11754
11501
13393
5742
6310
17106
2424
22017



6611
28211
17544
28373
1932
8904
28621
9362
8894
18421
2186
8390



11593
23976


474:
7357
21359
8847
14322
27450
6412
18367
3895
23298
1735
9395
790



1632
5157
10982
7522
5934
6755
15043
702
5707
16981


475:
3744
2450
26907
24268
6387
21543
27201
25458
8487
4208
17673
6162



467
21400
10103
26773
23524
1386
5099
3486
23059
30021
20480
18160



18918
18033
25273
3273


476:
27272
12345
3614
29499
10273
17627
27894
21679
19957
25636
14105
23973



22438
23520
3033
29083
24228
7931
2119
28793
7454
666
13512
16877



29640
24014
15267
17919
18018
8307
1348
5027
7278
26242
27881
17269



10091
15877
20047
3998
11519
27820
11364
9296
30371
28924
7745
28515



16257
6234
4854
23517
4861
4858
27270
27266
9307
27264
12149


477:
21573
6136
6726
1002
14014
24915
6875
17885
17296
1780
14580
21574



5809
23106
15893
28179
28204
28200
23235
28239
8085


478:
19911
2933
12176
5732
5566
22181
894
19342
4738
27202
8172
20527



20835
13886
18863
10895
30148
6550
16993
17604
10705
12703
25457
26504



20609
14506
16453
25435
22687
22745
15565
28365
8246
26226
22781
22609



28074
10378
25187
20593
10975
29843
28154
30166
25360
29090
25810
24964



7645
29316
7379
11901
25060
20120
25382
22811
19983
25469
12906
16564



16601
15729
5105
13643
8303
25194
9769
15291
17892
16383
3415
22356



21682
4193
18106
7989
22804
19305
28115
25199
13138
27283
26870
12445



2451
14975
12959
23717


479:
23860
12429
3776
14164
24870
10068
13531
9524
10508
16691
13768
10861



26460
17256
27208
13814
16726
26667
10023
10054
9078
18969
9074
18965



9043
18963
9039
19494
13757
17613
13758
13798
13763
27205
27206
26682



10233
13810
27848
15447
13806
6552
17603
16684
15856
15986
11356
9077



1929
7466
1069
28585
29621
740
12011
14644
14666
12083
3261
14690



10918
24952
9964
11423
4560
6851
4501
17463
28611
657
10879
2083



26235
1171
10878
29468
10910
20924
10909
25250
2827
6201
26981
1290



16544
12724
4260
8092
15444
11269
23635
18854
21138
8537
7555
9139



27937
11964
10614
1006
8279
2557
16771
18694
27789
11132
15363
4333



13764
13803
7681
9512
10355
15473
27183
13848
28837
15511
13229
13417



22242
10321
15312
12102
1589
28737
10288
15365
15293
24826
17630
23146



26660
10361
15509
27350
10295
22669
6293
5558
15294
15366
15296
15370



10291
12562
28309
17928
17925
13679
15321
17966
15397
20092
15324
15399



26662
15332
24590
15405
28957
13849
3248
13812
13809
15413
13105
25405



19835
20576
2226
10293
10298
2149
18471
15318
16277
10882
18022
11840



23758
23761
28953
12536
12537
12207
26681
12204
2922
4891
22854
10880



10886
25924
16591
25423
1729
1732
10857
28984
10359
15481
1536
15118



8737
8735
29936
15551
10859
3924
23016
3459
10911
30283
10363
15256



18628
3841
28560
19197
17400
7515
18058
16349
19235
30238
30024
26335



2069
29915
1134
13811
13802
13797
25698
3007
8289
30023
18238
15388



16397
9379
8995
12328
18477
10491
6603
12468
4769
5317
10602
18836



10482
7711
6508
11646
24479
2203
3075


480:
6859
12279
15737
28496
21769
14969
27180
9733
24403
11636
10570
1070



2798
9970
10415
11236
8470
8345
19642
22539
22512
25445
29247
26768



20252
21978
15292
30404
30111
29999
11284
13291
16756
1193
1646
19831



14916
19300
9340
30226
5046
13014
22101
14185
12066
2705
7024
24882



9683
27483
29883
11174
19960
14502
13090
12155
15071
23104
19917
10129



12646
6199
13887
20888
21538
29787
23970
23967
21684
21683
21688
30159



30185
24444
2588
2255
12746
23378
3834
3815
3819
25318
18870
3821



20089
19266
20090
20084
6102
6127
6098
6128
20093
12645
6092
14127



12905
23180
12647
12611
10868
26743
29974
6070
15868
8589
29181
20707



18074
18072
20031
9895
9891
9893
19280
22865
17827
21935
20386
22156



17976
18076
23593
11032
3679
9611
9614
26018
25179
25756


481:
5332
23342
24029
27571
23765
18921
3620
17462
13455
17803
24364
6672



2289
23193
5356
10089
21592
28489
26479
29205
21994
22730
22035
22858



25290
17216
12721
9211
5605
20033
20032
9069
11834
29638
22246
11158



26998
13971
11387
21456
5539
11547
9354
13365
19987
17274
25427
24426



20383
27342
22310
29745
25026
23952
26499
9378
5368
12997


482:
23286
1274
8225
27981
25684
25757
16457
16452
1391
12214
23260
23289



1744
11105
11360
5199
25720
25688
14705
6709
6649
16261
22979
22977



16994
16270
2059
2009
16265
20642
23803
20644
15180
15175
4941
4914



3758
4703
4713
16785
10080
16509
27451
6582
12093
3831
1155
9438



17429
4505
9638
12510
11369
12332
11407
12298
12366
12270
11488
11530



12277
12432
12339
12269
17458
17460
19786
19822
21082
19790
19696
19761



19793
19758
19719
19935
19903
19827
19930
19868
21151
21148
19763
19928



3788
4668
4665
4699
4673
16783
4824
16782
6878
6991
6910
6932



6936
6940
5992
4628
3791
4740
4743
26710
23228
28484
14653
14703



17093
15617
15646
15668
15641
15642
15670
15639
22762
13093
20149
2079



4751
4775
4982
6024
6061
6868
16810
6837
5982
6907
26794
25144



24355
5247
3727
3754
16757
3755
3784
3787
4632
4659
4662
4707



4746
4749
4830
4834
4879
4909
4907
4912
4944
4945
4980
5768



5792
5862
5899
5938
5942
5975
5986
6059
6091
6093
6099
6869



6897
6902
6942
6961
6964
6969
3748
25626
18897
18900
11659
3110



1332
23097
27977
1190
15240
5284
5204
5307
5306
5310
5201
5331



5329
5234
5232
5226
5278
5236
5239
5277
10149
2306
12349
27947



19479
19484
25020
24994
14113
14253
14109
14251
14282
15069
14138
30160



14321
14278
19566
14326
14195
19682
14068
14161
29739
10181
17666
10210



17706
17711
17670
17681
23292
16447
28736
13813
4269
8971
24172
10784



6581
21799
28030
14647
5282
27990
28015
16419
5909
5945
6619
14800



17524
28146
21825
21826
8124
8126
8159
8214
28025
28023
28020
8218



8196
30192
4794
15242
14452
2511
25359
13419
1836
2576
16666
1428



1423
19836
1910
7820
10218
20683
29045
6026
6025
16449
4481
14869



10745
11813
20059
22400
11648
7691
16414
8183
16455
16412
16460
13181



2994
2965
26527
16462
11863
7168
20675
16443
20678
967
17461
2509



11502
7375
17492
28280
6553
17455
2264
16409
16416
16418
16407
16444



4844
14779
20794
12379
7963
19990
15714
670
11705
25327
26672
28323



23256
17560
18542
22080
13354
13352
21460
21463
14532
29577
29581
29580



29552
29576
29541
8329
6616
6645
6642
16406
5279
20860
3939
8558



3973
4297
17449
19743
8597
25495
25466
23522
22778
809
27528
1300



23760
29578
25760
25761
20932
11894
20864
9396
3800
15568
9826
11866



20815
26007
7525
8113
8080
19979
20833
20882
19973
20991
20988
21040



20992
20962
20958
20922
20875
7061
20135
14531
6555
21597
21821
7008



19716
7001
19725
13816
19729
19765
25693
21823
15215
5081
20680
8119



12128
7002
5206
25110
5305
16506
4866
4872
4875
4869
1977
15074



15072
15105
23221
23227
18915
6585
4781
15238
28000
27811
27816
27832



27962
27900
27901
27903
27966
27969
27994
27907
27909
27835
27840
27842



27865
27870
27873
27877
27808
27809
27810
27999
27925
27926
27879
27930



28002
27935
27938
27998
27958
27959
9095
3804
417
416
22867
17450



4731


483:
23286
11758
1274
8225
27981
25684
25757
16457
16452
1391
12214
23260



23289
1744
11105
11360
5199
25720
25688
14705
6709
6649
16261
22979



22977
16994
16270
2059
2009
16265
20642
23803
20644
15180
15175
4941



4914
3758
4703
4713
16785
10080
16509
27451
6582
12093
3831
1155



9438
17429
4505
9638
12510
11369
12332
11407
12298
12366
12270
11488



11530
12277
12432
12339
12269
17458
17460
19786
19822
21082
19790
19696



19761
19793
19758
19719
19935
19903
19827
19930
19868
21151
21148
21112



19763
21144
19928
3788
4668
4665
4699
4673
16783
4824
16782
6878



6991
6910
6932
6936
6940
5992
4628
3791
4740
4743
26710
23228



28484
14653
14703
17093
15617
15646
15668
15641
15642
15670
15639
22762



13093
20149
2079
4751
4775
4982
6024
6061
6868
16810
4978
6837



5982
6907
26794
25144
24355
5247
3727
3754
16757
3755
3784
3787



4632
4659
4662
4707
4746
4749
4830
4834
4879
4909
4907
4912



4944
4945
4980
5768
5792
5829
5862
5899
5895
5938
5942
5975



5986
6059
6091
6093
6099
6869
6897
6902
6942
6961
6964
6969



3748
3751
25626
18897
18900
11659
3110
1332
23097
27977
1190
15240



5284
5204
5307
5306
5310
5201
5331
5329
5234
5232
5226
5278



5236
5239
5277
10149
2306
12349
27947
19479
19484
25020
24994
14113



14253
14109
14251
14282
15069
14138
30160
14321
14278
19566
14326
14195



19682
14068
14161
29739
10181
17666
10210
17706
17711
17670
17681
23292



16447
13249
28736
13813
4269
8971
24172
10784
6621
6581
21799
28030



14647
5282
27990
28015
16419
5909
5945
6619
6614
14800
17524
28146



21825
21826
8124
8126
8159
8214
28025
28023
28020
8218
8196
30192



4794
15242
14452
2511
25359
13419
1836
2576
16666
1428
1423
19836



1910
7820
10218
20683
29045
6026
6025
16449
4481
14869
10745
11813



20059
22400
11648
7691
16414
8183
16455
16412
16460
13181
2994
2965



26527
16462
11863
7168
20675
16443
20678
967
17461
2509
11502
7375



17492
28280
6553
17455
2264
16409
16416
16418
16407
16444
4844
14779



20794
12379
7963
19990
15714
670
11705
25327
26672
28323
23256
17560



18542
22080
13354
13352
21460
21463
3023
16390
14532
29577
29581
29580



29552
29576
29541
8329
6616
6645
6642
16406
5279
20860
3939
8558



3973
4297
17449
19743
8597
25495
25466
23522
22778
809
27528
1300



23760
29578
25760
25761
20932
11894
20864
9396
3800
15568
9826
11866



20815
26007
7525
8113
8080
19979
20833
20882
19973
20991
20988
21040



20992
20962
20958
20922
20875
7061
20135
14531
6555
3548
3465
3461



21597
21821
7008
19716
7001
19725
13816
19729
19765
25693
21823
15215



4827
5081
20680
8119
12128
7002
5206
25110
5305
16506
4866
4872



4875
4869
1977
15074
15072
15105
23221
23227
18915
6585
4781
15238



28000
27811
27816
27832
27962
27900
27901
27903
27966
27969
27994
27907



27909
27835
27840
27842
27865
27870
27873
27877
27808
27809
27810
27999



27925
27926
27879
27930
28002
27935
27938
27998
27958
27959
9095
3804



417
416
22867
17450
4731


484:
12506
14885
13628
27161
9283
29508
5274
16688
18995
18075
26869
21530



18055
626
4352
28798
14098
8518
22602
1223
14754
29635
15057
13180



22594
13466
12497
14620
27130
5420
28117
26031
821
18817
2441
9541



19961
17159
22190
22154
28078
22441


485:
14198
16733
17932
870
7072
866
17930
7071
16069
13851
13461
13464



14055
12701
12702
419
19755
8312
13470
8308
18143
19044
9845
28935



12911
12908
9746
12378
26844
6279
3256
29096
6492
6491
10340
1927



1926
14725
14724
5210
5182
16911
19053
10053
15107
10052
17902
18728



15861
21008
27672
14921
22004
25826
27570
30385
24545
7602
11773
25012



2106
29882
5639
8632
19341
3676
5630
8591
13801
17969
18455
7576



12282
29080
30010
25127
8356
9350
10154
5237
9923
12796
8187
29896



5402
643
678
24362
24361
15888
14391
3369
3240
19906
26063
23740



25138
23741
26062
25136
12643
4304
13273
13304
11196
30067
3274
7355



14440
5431
5433
12373
26674
13025
10728
19975
19976
9320
6309
13989



13167
13166
28931
28928
27390
2736
27297
28745
7015
29168
3670
5562



12710
21159
16719
1225
28194
28348
22794
9021
15530
5874
9984
11413



1550
422
19877
19878
26951
26948
17946
17941
13047
2877
2996
17097



17095
12242
18956
15056
21779
24579
15055
18335
24735
18201
2127
25041



1128
713
5162
5181
15588
15582
18293
7479
7474
10639
10640
7629



27675
7385
23019
23020
8438
25592
20405
15584
15585
6639
9862
19958


486:
14551
23240
12125
29926
21428
10051
10049
16359
17521
1303
1304
14480



4855
23766
21027
1673
16070
3990
16377
24434
2164
15264
21647
7979



1576
19535
6020
25742
30458
26519
7968
4483
5371
8892
23905
10500



5400
12858
16857
14546
19747
11568
13536
27245
19603
18653
14485
25726



4041
3096
24513
24510
24516
11995
4870
23214
5717
17916
18617
18851



9961
15421
22688
22691
9793
23634
17356
15933
29805
18191
10215
3190



4287
13779
22930
15197
26185
23217
24874
10212
12489
12493
17035
3884



21643
21639
2122
8139
28008
30099
16747
14130
7655
26274
16059
2597



28325
2041
3099
9480
17079
24011
24010
3576


487:
17778
10938
18908
15653
17688
13066
20725
13107
15112
13722
27140
14271



20967
11905
9749
5780
24679
13585
22476
9385
9674
21773
28461
25983



6602
18801
7941
7946
1594
5716
29759
26813
14884
17561
22668
25577



21619
12686
6466
26619


488:
6934
5852
24700
13371
8459
3747
4220
18103
30243
17039
9865
20065



12317
27021
27008
15166
7906
9349
10894
15908
16759
16761
23777
15799



6271
10631
13844
25051
29405
26935
19945
15114
8013
12127
4033
17959



13939
2886
14702
12707
5269
9267
4958
17664
1724
12344
6415
6807



3257
7598
10613
19411
26748
1992
7040
27803
16343
5065
12921
10829



5021
10016
17191
23742
3992
27542
15109
17148
2046
15557
12552
11673



28667
6381
11784
25877
2496
3337
27363
11872
9602
8203
24744
5064



3835
13654
17071
18386
28277
16239
14763
19665
7258
12051
5718
22306



25102
20081
29020
25347
18098
27391
22157
27398
19579
27053
27866
9775



16206
10692
25358
15063
17503
29653
26785
10424
3339
2406
11831
10224



24533
5120
13130
21595
12025
3353
6405
7519
9734
28998
23813
19217



9783
28678
1286
838
5887
5691
18558
15504
12079
4498
7653
3528



7642
28459
25809
25548
19298
17459
13182
26486
29795
23090
17891
18954



4645
18236
19204
5487
8283
11812
21660
1117
17055
29716
14442
10496



21065
5503
9298
25967
1280
7920
9741
13660
16199
25963
6316
30277



12213
27036
28375
20106
2717
30214
27299
24886
26991
29531
29271
22861



8625
7468
8255
14243
6351
14811
22713
29527
2919
22390
8698
19330



6632
19192
710
3907
26984
15169
12444
29263
14484
28593
29506
7879



13416
12267
1217
9851
22900
18698
26583
7958
24239
9331
11515
5257



13793
18597
20619
29615
27843
24944
20205
16174
24378
8293
13370
29840



17228
904
26067
19551
7384
25432
29810
17732
21533
26359
24928
4619



9825
25056
14541
18499
7473
11627
25774
13496
30437
15774
16279
1756



955
29175
24856
23657
21726
16327
23687
25692
12364
14610
10922
12781



18832
4928
17112
28449
23898
19296
25621
24240
27574
3781
21380
19463



13012
11969
30407
12672
10097
22219
25764
22342
25852
15736
2000
29637



10127
30271
15993
13062
22971
14468
22662
10390
12082
4963
3646
27034



1442
9686
21140
5218
24956
9764
13049
17064
1760
15712
24825
2363



27050
11796
22872
8970
2404
25310
6023
11475
4253
3054
8771
7930



27405
28680
16015
12716
3618
13826
18616
12845
5865
20843
1048
25315



15144
13425
18780
19288
28088
27434
4470
29946
7863
11116
10260
18709



28499
822
26230
23951
15108
10756
23572
23571
29153
13941
5801
20856



7689
10398
25699
22956
19040
25842
22135
29606
25475
23445
6495
14739



2594
2600
12307
12310
969
18583
24045
18005
7132
10960
25818
24481



20581
24190
27395
6275
12591
23422
29543
22312
7404
27708
27710
11837



17583
28903
20133
28862
8117
10574
26353
8071
22965
5897
24749
25079



25069
2687
10452
19679
25844
15015
9439
7669
9765
21477
6421
24068



15381
30105
30328
26862
20374
4069
5424
25846
13929
7370
20412
1372



18112
8200
12667
29930
11161
27707
1440


489:
20634
28717
28716
21425
8439
6534
2346
2341
2344
2293
15778
9185



23756
17947
12827
25538
12433
20855
29677
8406
26074
21476
7021
4549



29681
23174
28456
25965
24156
1279
27655
3825
18526
21883
7426
6057



23419
21753
784
18605
6809
6812
6814
12657
22628
3324
15750
21556



23728
27188
24044
23711
30241
21654


490:
22876
17843
24061
23547
9277
1484
8780
8333
20353
7228
3622
21977



21710
30060
29991
27220
23136
4216
22981
26646
27541
15497
20462
3290



16144
8335
24931


491:
6855
23827
6260
10531
9711
3036
11396
16702
22401
12385
17132
25508


492:
4073
4074
23394
2844
26262
26265
4274
22555
4100
28493
28492
18874



21520
2408
2403
23481
22482
5544
23403
29897
28529
10837
23686
10611



4063
19971
20002
7073
13723
13721
19412
29019
23907
8695
10864
10862



25433
5314
29590
13618
5736
15587
2366
15756
18027
18644
25869
13478



6691
17779
8272
28495
24521
28573
21337
18951
18989
16902
18985
18980



1933
26747
29391
14309
1994
8624
13325
25476
12360
15080
16289
9817


493:
26288
10403
17815
27594
1569
20525
6783
7521
15205
4292
8103
8104



19076
12966
10380
17314
26092
23329
24319
21471
27171
14184
24840
10915



25210
10612
26989
16694
10183
24937
3284
19533
14871
4215
5097
6378



7437


494:
8146
26918
16137
14667
22888
2925
12977
12675
17605
11907
10816
1511



8686
18555
8371
15387
15980
15946
19594
4424
21192
12206
20285
25643



16094
9631
1060
29094
13890
20604
9287
29846
15526
23496
21495
11465



12889
23533
8128
27131
24321
9105
2632
20012
8756
24369
20496
19096



10775
11703
9598
3535
5180
12894
11708
22228
21030
18594
11453
23069



2643
5685
1293
12820
20795
28225
12437
7052
4066
10939
18748
4720



19338
16483
16203
5024
4488
21596
1367
13527
20996
1078
29159
16928



24078
25941
16065
14834
21133
8722
28457
14649
24650
23373
22767
10198



26501
29393
22158
27479
18609
20951
3493
21315
18940
12737
26959
8053



26928
5760
1288
16609
19882
5569
950
10136
14613
13906
2222
10576



3452
6219
4225
11561
16022
22121
10333
3582
19168
18619
19785
432



21894
16650
13413
683
22074
20491
28193
25961
25977
15634
12162
15814



18491
21190
25232
28527
6789
19362
26832
29018
7067
22568
18361
13991



15127
14165


495:
15510
23745
869
9643
25537
5240
19233
21470
18859
19426
13254
15595



24634
19005
14331
653
7732
27035
14662
4170
24736
5337
21591
28042



22227
7428
8805
8731
19101
16690
4016
25800
22905


496:
19486
25309
25317
22542
9999
22071
883
5638
3062
28160
16341
15955



24747
9805
14528
8069
760
7165
16988
8557
11439
24893
22650
26721



25863
1985
17347
11317
23596
25314
7739
25313
12725
20274
6438
20401



23762
15395
15054
4255
27514
14867
2154
26627
19008
17483
7844
13878



29451
26459
10762
5531
26974
19145
17027
8316
24676
9076
9129
22796



14785
28284


497:
30239
18561
14887
24891
22826
14478
18533
18532
17376
17344
17582
17329



25301
3737
17338
23847
16831
25930
14584
11924
26712
20144
12700
5186



26320
19185
6655
21737
2191
25804
6123
10800
6615
17331
16315
7441



12516
6706
30268
14663
8306
7017
3322
3317
28649
17345
17343
11428



11431
1923
18510
24667
18508
25114
28417
20343
1750
13368
27910
17731



24664
12147
28463
15891
8025
7493
27000
11916
18119
18123
18069
18031



18104
18062
18129
17938
17907
18026
11917
18065
2558
20664
21108
19670



19675
19668
5494
21110
19727
6159
9900
12046
8215
14188
23583
23587



23586
23588
27477
10150
21536
13003
23199
10146
24950
12276
21852
9943



10122
885
7266
27079
9759
12664
5851
4962
25528
17777
29865
26434



26431
29863
22893
18572
806
2260
6156
21650
4130
24563
6186
6185



842
24330
839
24694
10227
6161
6164
24692
21616
4132
1666
6189



24281
834
24659
20802
24657
24662
7550
27555
3566
27461
7976
5684



18463
22676
18830
18827
18792
18826
17884
5351
5132
21888
14842
8107



3297
26178
10161
10065
6762
11095
9619
3164
23438
26911
23249
23411



18794
17906
18803
18799
18806
17905
5828
26450
2627
13443
4439
5581



18831
22897
3640
5587
27055
17059
27312
11862
3882
3917
3851
3886



3878
3854
3893
3913
3847
13402
19939
21583
21322
13820
1604
28218



8042
17341
1207
21475
30187
23361


498:
12651
27676
16874
28937
28103
27096
6937
8281
25899
16470
20876
19505



1535
20819
17014
22996
13260
29855
12078
29568
28910
8811
12735
22292



3226
14027
3224
14589
14588
8393
1526
1563
25703
22950
21732
25276



10367
14086
17499
6711
27501
23532
5853
7098
30157
6852
10875
2857



14202


499:
24678
24901
2909
24878
27828
10766
18202
27168
24561
28398
10999
19658



22042
16881
5846
2027
23338
27825
7667
11643
22204
8174
12879
19312



25910
30439
29607
23632
29585
29613
14738
27767
23975
14683
30467
17756



18041
24199
12034
8938
25146
23162
26992
9548
22124
17337
2395
1351



18222
20577
2645
12926
25752
14926
12719
12962
8723
18964
10682
16372



22803
24343
13390
13190
11927
10094
11981
10100
10092
10018
11934
18269



11087
6728
5117
10021
13926
19521
16790
12607
16794
16795
21942
16792



29599
14334
22507
23021
22161
18485
28919
6721
28631
6308
22701
25478



22553
2349
22695
22663
29089
1154
14925
23534
1033
21547
23649
17047



24711
1215
11404
6106
17984
856
17980
6324
22985
21129
820
7300



23309
7042
12817
8347
2271
4688
12972
27652
1425
19508
18569
24684



29435
25489
24680
23876
24655
24651
6752
18482
9035
13590
9523
1873



6874
26068
2870
25332
11743
27596
9088
8428
9072
9086
28298
7686



2548
7575
23391
28294
11150
23767
10281
25353
16357
7994
29948
23712



24940
21067
19517
25831
18925
20908
28458
27573
26558
26529
7690
16502



16507
13795
16510
25078
26294
13221
28698
16026
20142
15378
5345
24428



24395
10286
12062
12059
12063
1179
13013
1176
7180
2385
7154


500:
12633
5576
26464
6939
5436
6903
19289
12073
11239
11220
5207
17109



24162
21590
19286
5425
5430
27381
19320
5610
30375
28547
21508
15517



694
24168
15269
30444
22766
5774
2159
22995
14625
27546
16625
25748



18896
29115
2211
22848
6662
15338
18141
14813
12188
4186
17651
16654



22596
24543
24139
24171
13070
14145
9867
22339
6261
7342
29017
8122



12863
7811
20259
12960
24163
15890
24933
16181
844
23831
24134
3722



25962
3343
17871
18224
24138
13856
9840
17246
5864
29945
28973
9848



5133
10174
26116
16948
21378
855
19993
11003
30218
8090
28428
12644



14159
4657
7704
7703
24137
16308
14049
13022
13027
12177
2556
7496



13569
884
18847
16724
13023
14144
26725
23143
3276
2419
28613
23690



23691
10724
2410
20847
6017
26851
16062
19848
10284
16302
7117
15902



16224
27646
13245
16310
5202
15607
19751
10030
16301
16282
5225
7893



7923
7921
1983
12399
12402
12401
12398
12397
17040
9339
8652
29039



11778
13024
16120
6482
13004
13019
26633
6479
26162
20447
21352
23855



993
14542
2101
992
25595
13001
13007
5205
6181
11536
13905
17769



22987
26761
9483
24331
16312
9484
20036
20039
20073
1039
2389
29034



21350
7738
9401
24790
21351
1082
3958
5534
20602
7626
18581
29001



9368
24164
9476
5233
28865
8163
27974
13483
9874
13645
27614
15344



10055
28778
10085
908
26856
14272
27905
23139
28728
13538
5203
5175


501:
22063
26045
16912
30127
29320
29303
3541
26289
19539
21860
11235
5935



12869
17821
10527
11451
8244
9118
24250
1287
12226
5618
25068
10889



2056
16966
10027
11990
14888
18855
14742
24259
5720
9122
27531
18352



14061
13206
17734
5040
13026
12003
14560
17261
17144
11790
3651
17116



11170
6990
5807
27796
18507
16941
19250
22019
2438
5374
17384
7194



25109
21461
5102
30370
3332
5229
8370
1950
21814
6787
25734
26586



14652
25785
14530
30381
7252
27175
2028
22652
29101
14959
17276
4979



30282
23724
8395
508
22852
29157


502:
13141
24663
5432
4653
22175
1044
12194
7412
19489
2082
25029
9904



18731
18122
14783
6856
4391
23499
15960
18126
14229
6166
17114
10579



29459
23550
16448
19706
25118
28479
9093
14062
29804
30189
13089
16932



26848
29521
29522
29526
11199
25321
26944
19367
4460
19368
26955
26979



5715
8189
10936
16430
10232
11989
10845
30364
18875
27814
19118
1717



5766
19577
20055
26454
13098
27931
2418
5803
28217
2766


503:
25958
21576
13591
25389
20630
3483
22382
12692
10229
11434
6540
15815



12873
5034
17995
9790
6967
28265
2830
21768
9648
3858
16066
18411



2300
2126
18598
5073
25603
24984
19699
23754
29387
7972
7378
27782



28686
14100
20872
4178
14658
25949


504:
15660
8610
3591
26937
28126
14474
5858
18044
14032
29395
24710
9902



15749
12526
3271
4975
25111
27049
2193
13784
29244
29594
11386
1623



6768
5328
13226
24129
22632
2427


505:
18237
23960
23994
23991
24179
24180
24076
24118
24146
24149
24030
24181



24184
24209
24210
24213
24217
24110
24232
24034
24105
24236
24238
23998



24071
23989
24241
23996
24178
24037
24027
19800
29453
11999
15201
18953



30110
7915
24337
23241
30053
28316
3763
12309
2522
21405
7212
5850



23549
623
30338
24161
22828
20729
17637
3270
7313
6659
5092
6952



4347
26191
1042
9629
19472
26754
4273
13112


506:
26977
2097
28521
6741
15677
8890
26152
6354
2916
26592
8078
7651



2399
5735
895
14151
10748
1302
12141
9457
10877
20297
17304
30352



17639
19667
21703
24800
18865
30149
6282
23510
25032
28508
15914
21931



13957
962
15356
12958
4817
27144
25222
26503
26593
10679
30007
25456



20427
14505
20490
5443
18592
25401
2854
22437
22685
22037
21088
28656



14978
26195
22751
18797
6460
25369
13440
19144
5499
5573
26606
24983



23850
21698
3319
14701
21713
24422
13029
22785
14283
11353
21563
24006



8150
1263
2765
24262
16099
2029
1975
7552
28441
15323
10935
5160



28101
5861
21800
18259
24392
27733
27620
8037
25834
30194
25637
12199



3192
2814
2314
16707
24266
712
5948
15772
10721
25413
19780
4500



10647
16680
5863
16892
24799
10806
18073
5725
18316
7158
23032
23819



21839
21691
12086
27923
1875
29050
24051
15808
7277
18233
20830
12116



6296
6757
11411
23523
13225
30259
24347
23380
13451
15503
18219
13214



12800
24183
14291


507:
20779
24939
5311
15671
2296
20652
29109
28620
3206
3200
28629
4589



24485
948
15172
7128
3922
17466
17990
21375
3142
1846
28139
7341



21244
21287
21295
21297
22145
22180
22178
21254
21332
26081
3066
3105



4117
4005
3965
4002
3971
4040
4079
4083
4112
4037
4013
4051



4085
4114
4086
3968
4009
4044
4048
4011
4091
4118
3169
26086



3101
2953
16273
3143
4119
4140
4179
4141
4149
4176
4181
4142



15489
3165
4185
3130
4146
2949
2980
2981
2985
3203
3025
3067



23283
3092
3167
3028
3090
3132
3172
18566
5161
28978
21102
15354



23047
15472
18591
21291
12821
21164
21198
22103
22083
22143
22110
3021



3020
3068
21252
2690
13702
21330
2517
9247
19405
11833
20755
12527



7417
23149
3888
8162
10621
27724
9473
27459
2829
9059
3318
14598



6399
1832
14639
5075
20574
7540
3254
6498
15079
25237
25717
18738



27099
2094
14428
27712
5129
8845
16967
20466
19980
15767
14977
23713



5820
16186
9309
3216
3940
24473
10630
29434
849
5322
17378
772



28623
22245
11137
3523
8751
17963
24926
2367
4290
21608
16227
25630



3812
16692
29235
14220
10781
15605
15245
4837
8579
13179
17910
19812



3127
14425
2549
17607
19705
16842
23437
27174
11157
20352
12148
18582



13552
19628
28927
11275
8650
28754
21601
21605
18537
12838
8054
25377



20187
10814
27375
21086
14376
26151
26177
2335
1920
23181
3568
11054



26826
4974
4438
15277
12940
1980
29047
6511
6690
11979
29984
24503



15358
19823
22832
9415
27615
21329
27853
23178
28845
8706
1212
19502



9820
9726
10243
15903
4921
24963
19457
12330
28572
19685
26339
29786



917
21450
14732
22089
923
14536
6343
3663
17098
10720
20472
29471



8023
19291
1835
1124
28988
13271
21913
5017
6297
12797
10767
17295



13111
3431
21493
21247
13672
29339
17698
20044
4482
18358
5822
21385



1709
5937
1330
23764
8382
3231
8583
12115
19190
17480
5614
8467



26165
7280
14035
4957
25733
2168
4261
28085
19259
7793
12844
19678



20592
25443
28577
18775
14964
12904
20324
12253
11299
16242
2064
21929



21544
18318
25219
1545
19720
24063
9881
26788
15537
12998
3693
7888



30188
12424
12363
16914
5342
8474
13659
24546
960
14126
1740
22524



29899
24833
21975
8271
1931
7069
18183
19392
13338
9699
18568
14080



19020
20524
20974
9116
24390
25530
15480
4211
24148
8207
21327
3097



4214
4187
3928
3960
3956
3932
3930
3925
26618
15185
27318
16698



22055
28710
28706
1114
25182
17940
22641
10226
11018
4497
22234
20826



5594
29126
10805
28558
26651
21427
6126
9791
6278
1229
21117
21155



21163
20666
21321
22927
11026
11741
14905
9728
13522
26927
21896
24147



1629
14177
30195
10510
18812
18546
23087
30245
27802
4459
1046
15919



7624
5334
12548
27606
3816
24969
11126
16924
5632
20968
22081
19036



18590
11125
6110
3546
1041
6377
28952
29794
11921
23034
12741
14655



4538
21490
18565
3556
5419
18579
19922
2707
1491
1497
21323
11154



26574
29960
13760
2987
23801
22077
21200
21249
21122
25421
19768
21836



30337
22105
3030
3061
942
3171
9485
26196
17053
23416
15062
25260



22146
17950
15199
23867
28735
10559
23054
23147
3136
3072
6566
21207



9274
22079
25293
11881
7338
21160
21121
7420
891
21201
22107
22241



13174
11127
3199
3197
29237
18539
17286
9115
1483
27826
24785
15035



22974
11803
12237
25289
26718
26453
1870
15674
11151
21791
21204
937



2992
24040
16384


508:
26045
16912
30127
29320
29303
3541
26289
19539
21860
11235
5935
12869



17821
10527
11451
8244
9118
24250
1287
12226
5618
25068
10889
2056



16966
10027
11990
14888
18855
14742
24259
5720
9122
27531
18352
14061



13206
17734
5040
13026
12003
14560
17261
17144
3651
11170
6990
5807



27796
18507
16941
19250
22019
2438
5374
17384
7194
25109
21461
5102



30370
3332
5229
29593
8370
1950
21814
6787
25734
26586
14652
25785



14530
30381
7252
27175
2028
22652
29101
14959
17276
4979
30282
23724



8395
22852
501


509:
24805
6339
29491
7984
17081
2370
13507
12097
20345
20830
15927
6296



26465
24548
11566


510:
23567
15328
22917
10973
28600
1066
18353
14143
13169
18901
23209
26996



6647


511:
23773
16720
20388
16728
12602
14397
18489
25591
21502
27456
7172
9936



18128
1038
18270
12642
29770
13323
6027
2757
26790
20536
15896
4247



2291
1798
23805
6374
24459
27464
13913
23529
8440
15263
9459


512:
27904
25431
14789
14183
22929
27218
4526
6673
9061
23949
17881
1080



25209
2770
23334
23344
20251
27328
21578
12878
28058
22801
17685
30119



7443
28228
28916
18235
29133
19893
21663
24150
2673
11043
27772


513:
4331
23464
11950
28889
3508
9715
5921
5029
5212
17167
6776
14171



26980
4402
19061
6538
19039
8820
12841


514:
25583
25602
25606
25629
25635
25666
25668
25671
25675
25704
25708
30450



19353
2616
2612
11581
2606
3607
2652
2656
4666
4562
4595
10715



10757
10723
15148
7011
10145
10555
10562
10885
4615
4614
10592
11306



11308
10950
11366
10981
11484
11491
11061
11066
11524
11067
11589
11559



10940
11337
17802
4867
15696
4771
15762
9196
16637
16636
9226
9230



16803
4936
17828
5784
11301
10012
29367
10763
11274
11399
11435
10139



10137
10135
11305
11363
11368
11409
11440
13737
22631
22635
14535
22638



14593
14597
22671
22673
21171
21127
21028
21175
20934
2618
2648
10795



10798
2619
2676
2675
2677
2679
5524
2681
2780
4637
3564
3605



29322
29325
4600
4598
10792
10790
5599
5600
29374
21064
20944
20940



20937
21061
20981
21126
21092
11767
21020
21069
22000
22029
22032
24069



24083
24120
24194
24086
24122
24117
24119
24158
24182
24186
24188
24153



24216
24219
24126
24244
24246
24249
24251
4774
4701
19529
19581
19532



3151
19583
19617
19573
19536
19576
19621
19661
19578
26326
26323
5703



19524
9634
14630
11338
10945
10937
10933
11094
11535
22003
16871
23011



13888
10687
10759
10725
10691
10751
23322
15023
16600
24154
24080
30427



19348
30456
25633
14773
7124
7178
7157
7150
7246
7182
7255
7101



7245
7215
7094
7118
7210
7156
7153
7286
7220
7186
7293
7249



7125
7151
7288
7326
7294
7296
7329
7333
8052
5631
3602
3600



4390
4392
4393
4385
4446
4452
4449
4422
4491
4492
4495
4517



4518
4547
4559
4555
4550
5523
5520
4718
5529
5560
5619
5626



5622
5601
11610
5667
5673
5669
5635
4457
4454
10732
5675
5678



7123
4852
4693
15678
4735
4821
15700
4736
4737
4741
4770
4773



4776
4806
15757
15786
15788
15791
15812
15817
15840
15842
4825
16603



16634
4856
16647
4860
16669
4863
16737
4894
4899
4900
4903
16778



16801
16807
4929
16832
4932
16867
4939
17668
4969
4973
4976
4977



17722
17725
5746
9982
10007
17788
17794
17800
5753
5757
5783
16703



10014
17832
17757
16772
16706
9973
9976
16739
17708
11576
11608
29646



19212
29648
29650
19215
29652
29668
19219
29669
29673
19240
29674
29676



19243
29697
19247
29699
19252
690
20263
29702
19256
29703
652
19284



642
20220
29734
19285
19287
29736
29737
30422
19322
30424
30426
19329



20265
19350
30446
19351
19352
686
20223
30453
20169
30457
20173
647



20228
605
608
16891
9952
10009
17765
9177
16643
9979
9983
14045



14016
14039
14040
13976
13974
13980
14012
14993
1358
4588
4584
4571



4574
4578
13670
13711
22598
22601
14564
14562
22667
16664
9182
9188



9223
9950
9954
10004
10761
10667
10688
10717
11500
11507
11505
14991



17532
1647
14787
14781
10730
13599
20410
2756
4380
29364
3612
29331



4522
4519
11579
11601
26325
4631
4630
4633
10824
2657
29369
29372



10693
4542
4540
4690
11333
11224
4733
4612
1900
10729
1898
1893



1896
11599
2714
5565
5563
5564
2721
2723
2752
2747
3571
3573



3569
4413
4411
4416
4420
4489
4485
4629
4603
4605
4660
4663



4702
4674
4669
4710
4714
11582
4704
5526
5597
5567
5596
13979



13987
21993
13992
13996
12409
13942
14015
1360
21231
14017
14782
14841



7853
26652
26664
11552
18066
18134
18070
18101
18206
13236
2768
20087



7036
18946
18255
18945
18264
13982
4581
4582
4908
11244
4616
4621



28178
4815
9947
9192
16893
18369
17217
14632
29645
19209
13830
14636



14637
13919
13914
13920
13917
13944
13945
13893
10689
21093
13841
13854



13847
17547
17545
20904
20906
20899
19127
19155
19150
27482
15098
726



2332
10069
17569
22026
14028
4404
4544
2336
9373
5634
19773
16355



9371
12698
14876
22790
2402
17017
940
939
10096
12386
14050
14053



12408
13921
750
749
747
782
745
13889
23094
1437
9150
15701



15718
16604
16769
4942
9955
17675
17719
5750
10034
15754
15676
14020



14046
24075
21228
13946
13951
14044
14024
21225
21215
21221
21178
21130



14818
13135
14805
14808
4966
15784
7918
1366
1369
1505
1403
1475



1396
1514
1389
1436
20903
14048
11618
10831
10827
10832
11612
20983



4694
11282
15719
15838
16670
9229
5754
8876
25306
19624
11594
21987



21997
14640
8051
649
610
20183
19318
715
20268
19159
10713
19358



19475
2718
2719
13891
23044
23072
23046
23077
6817
6915
6790
6774



19354
19357
13705
13708
13710
13714
13716
13747
13739
13741
13743
13745



13781
13782
13785
13789
14809
14784
13828
13833
14817
14777
13835
13840



4611
7376
7096
7374
7373
936
934
21135
20975
21169
21087
20978



10586
20935
21134
14270
13778
17534
17537
9633
20901
28711
27949
21098



4832
385
10134


515:
21290
5113
10834
24810
4054
3176
24225
12009
21368
7302
8961
6157



3943
9934
26831
10132
15895
7282
20949
16473
27284
8644
5550
15133



18257
24023
2317
6190
8953
20344
13069
2192
28175
12896
13677
1143



4793
10109
7604
3381
18047
3245
23727
30248
16113
23595
7606
6573



19236
21032
9806
28383
19431
980
16998
7275
2198
1289
23562
13235



720
26429
1312
7580
16923


516:
17638
29775
19546
7100
21078
29472
26206
14606
5866
21542
10893
23028



28069
9435
27011
4314
20175
18297
12978
2762
20143
6089
27189
27017



23784
23697
24689
6779
23568
16806
2628
19337
27084
30085
8185
16480



5557
1961
2421
18364
24072
10205
19813
12996
13094
7636
29267
15739



10609
16763
26659
2575
17702
15594
12665
29225
8278
9180
28016
29556



25090
24005
9907
28287
21642
24941
24942
5491
2270
16971
5215
5062



5059
5054
16969
22674
10175
10170
10172
10072
29929
10173
14928
20423



14922
8299
26409
3588
7503
26584
8043
28658
1029
12685
17300
9165



30326
7436
23962
28830
25587
12369
23509
9475
27204
8176
29489
23188



18480
2148
19133
24058
4847
582
800
10954
11217


517:
21770
30011
30013
5855
4865
5700
5706
5859
5791
5752
5728
5724



5658
4862
4829
5824
5795
4859
5786
5848
5821
5698
5670
5699



5664
22816
29343
15969
15231
3408
12434
6846
18881
5093
3559
26787



17007
6775
6773
9005
9002
8310
8317
6519
19404
21689
19427
7861



10335
10332
6295
5905
14072
9143
17653
20883
27755
15198
27238
27235



13829
13621
15190
11997
6232
3608
7261
28197
1254
12545
15825
15823



9461
9465
24609
15683
12603
29951
9133
5839
6831
17073
16192
11603



15973
15725
11616
6056
6054
18067
7608
29384
25428
983
9576
9578



7050
9539
17232
26208
6738
7303
9533
28961
9530
17883
15373
4706



15028
26207
8829
17171
6877
18002
6391
6390
23748
23746
23721
15226



28575
13516
13519
7513
1247
16864
16869
16860
15236
21128
21162
8369



4809
15959
4813
14803
23564
15975
23296
25872
28950
20617
20612
20585



20589
20591
1955
12092
29243
6887
23508
24581
26412
25814
25817
15223



24577
17336
17342
10786
10820
17335
9678
12419
6805
11847
11874
11868



11877
25604
14313
14673
25291
13488
12989
14149
18651
1448
21435
17469



15415
1104
12544
14219
6226
1162
12479
527
23565
8755
731
17150



23607
23609
1057
11976
12001
14156
13164
30387
15233
28285
20615
29661



8658
9723
22231
2124
5200
6043
7432
2118
3482
7434
3868
11931



11939
11935
23750
15806
15810
19068
17420
12769
12770
5300
5299
5303



23770
12285
25513
15834
13977
25515
23648
25516


518:
1766
25623
2634
8567
8546
8035
8030
28060
8033
13487
12426
12425



27566
16382
13471
13472
27829
21056
5010
7641
7722
17728
13546
9391



8454
11625
8039
26982
28645
2216
19396
12857
6428
6029
8927
26241



28882
2058
17137
23307
23313
14163
30357
1765
1758
22795
1762
1007



12696
20720
20722
21606
21600
20724
9877
8100
8623
9032
26511
15887



17511
19373
19998
11822
25957
20063
20812
20816
20818
24166
23982
8056



26481
24795
8640
22623
30201
420
14318
2731


519:
25197
11884
10001
9801
25850
3323
3196
26301
11122
19333
25689
27968



6238
2621
26736
12639
4174
23354
17388
27426
27427
10406
21116
23213



21243
29059
8253
8171
5008
4506
8673
5592
22265
8826
22303
618



16976
29729
2320
27010
3688
26173
14160
3350
14293
18290
15945
13015



4325
17647
17610
21529
27333
18279
29919
12396
609
6953
24793
9668



30204
3889
3892
18054
25062
20543
23442
16638
28168
28173
28174
5350



1914
11930
24089


520:
2689
14849
20863
7612
25755
3539
14404
22824
4568
24464
6490
16629



25849
20730
17593
26509
6459
5167
8574
8626
19464
14601
11531
8491



22866
26306
21750
18256
12236
11056
24205
15793
2311
4917
10189
18004



29165
15726
29715
26447
12181
13562
24328
6504
6665
22011
22044
20372



15347
14990
11045
5260
17373
20928
23859
3305
1833
3152
8720
1113



20291
22615
15019
21326
11382
11389
7281
24857
12221
26840
26877
26883



29907
19228
2630
28574
3931
22277
3011
12778
10598
7566
18722
20915



26581
19117
5375
28215
22769
1891
22538
20306
11808
17633
19319
9819



6884
16389
30016
23358
25759
5468
18990
18978
18976
10902
10903
4755



6211
24576
10925
22835
27922
1663
2816
27656
10912
22920
16952
15822



12193
15885
3916
16481
2090
5235
23752
17700
22846
22648
25430
15548



21672
9837
16822
10843
14758
23165
13578
15613
29761
18686
23160
24538



25011
28960
26247
22287
25246
3205
28109
19999
6434
5183
18813
25027



2793
28805
7455
15092
24341
24919
9394
26036
17100
16104
5255
17147



5620
10331
26139
11859
18267
20614
27524
22924
15741
21386
14368
18695



19625
21559
22647
12356
12450
10772
9946
24686
23039
18741
13749
19442



29388
1308
8573
23890
23397
25108
30435
6430
23944
8419
30418
29445



11313
21066
4729
16658
28771
17865
25008
17695
26533
24074
8734
12776



12772
1862
22372
25624
25628
21945
5561
10520
11880
9565
17739
24912



12352
18912
8247
11192
19227
1203
21625
2664
15404
15372
2035
30315



27995
2185
1860
15792
15794
28925
2471
27024
3479
7806
27673
10067



22566
3811
1026
5138
4466
4569
3659
7221
6890
10684
14880
11619



5480
14429
26775
6239
15506
11172
9263
4139
15183
906
23440


521:
24439
16617
16614
24792
25067
26220
22153
29830
15351
4278
9607
5481



8057
29705
18225
15593
16311
7831
20757
22578
11948
16905
6302
9978



21179
3762
13100
1232
8659
10956
10849
10707
16405
22360
17386
7766



29684
15545
23939
17526
23477
23696
26536


522:
1839
30065
26828
30001
23943
8081
3984
18730
8542
30087
20248
24377



21974
14359


523:
10402
30128
746
3584
9667
18721
18723
29525
27516
9370
4121
10700



1296
13641
9927
27125
23124
19608
15286
27538
9922
24112
13624
10702



27797
10324
23709
14911
3420
7361
28407
13623
26488
26461
20793
11323



14423
14337
25741
17677
25768
17701
25702
17704
17671
25775
25738
25770



25739
25732
25700
17713
17707
25737
17667
25227
11372
27567
3349
17768



5190
11340
12888
28511
2422
22136
22168
22126
22131
20529
20532
20498



8436
15800
27619
28163
28185
28153
10420
22213
22215
24436
22239
22308



22331
16975
18672
8807
22244
6725
26424
22206
22208
12134
12217
975


524:
17281
10048
1061
27298
12259
26864
1886
23852
20315
17733
7467
3875



11978
9586
14416
7416
28871
1882
659
21549
22329
25113
13687
17284



1383
18299
19371
21742
6152
29208
15547
6139
30322
19120
19782
19160



14435
26456
6188
25334
27145
19843
905
9424
3425
9195
25098
6204



3146
14405
28133
6982
4434
21377
21342
4419
20571
7344
26444
21696



22269
9663
17145
19473
2552
2832
8430
9879
14432
19683
14572
15648



8334
1239
13248
29497
2804
6376
15258
19501
13997
24414
9232
23983



12654
11371
22407
2084
8536
23598
9561
7507
4985
4409
27856
28041



19409
16741
21812
23098
15059
24965
25063
26082
21256
28959
8058
26692



8578
6745
14232
28084
2449
12813
17676
10804
27586
1953
13218
8651



27647
15493
6119
18920
20458
11219
22417
21848
13037
17669
22931
13993



3104
1081
14038
14557
18514
2788
2704
18017
19063
8770
8192
29482



21347
1781
10370
1138
4296
12660
10550
23699
12569
17901
24806
4573



8965
21557
24755
3840
9381
4636
9065
17542
9276
9017
10769
17242



11297
10547
24762
2961
5571
1158
2242
16293
23330
29902
17312
15931



16322
1032
17813
27249
23918
7162
11373
25463
7147
21268
11034
25050



1242
12854
13232
29259
13911
15250
29143
9703
22736
10087
24373


525:
23537
16291
16296
16251
2956
21230
14256
8499
12509
14266
16518


526:
17830
9810
13683
16742
14698
29777
8999
23264
12153
2738
23387
15161



23239
30139
27491
23265
30221
30219
30199
30167
30075
30031
30000
29975



30193
30081
30002
30106
30071
30104
2584
29675
14437
3246
29708
9081



3432
29413
5096
22761
8240
10515
10516
20479
11146
19360
28594
26990



5263
2477
22075
3549
11113
12361
14473
5884
7559
16166
3517
7701



16380
10828
6425
3522
8180
24430
2669
8851
23845
27485
4613
2378



3794
2741
2744
9921
19795
29025
28243
24346
7179
18979
9251
5665



29266
19614
4587
24441
1055
2362
2546
22856
13815
17041
12019
4841



24704
9885
15165
28995
17535
13542
11551
3234
13011
757
3501
12835



8633
8421
18328
15210
26966
24559
14125
2761
27861
13155
3039
7089



10430
27124
8859
15486
15462
14410
18061
29996
28883
5867
5873
5876



15484
7625
17291
23716
21341
25840
2881
21436
27316
25822
5844
25607



3550
22514
27847
28918
10920
5353
22284
14544
26995
5837
5841
25266



15457
15454
5870
3471
20865
15407
15412
28760
9839
15450
15418
15414



15420
25853
25264
20594
1637
13580
9812
5360
3463
2013
3472
23788



9748


527:
24446
29343
21407
1978
1970
15969
15231
9802
18390
18389
14680
19082



18391
11540
5093
3559
26787
9005
9002
8310
6519
20095
14072
9143



17653
20883
27755
15198
3721
29466
13621
6232
3608
11358
11361
7261



14788
30358
1254
2190
7520
24136
7731
25586
3524
17782
2201
15973



19038
12628
19001
2796
29384
25428
7050
7303
634
8672
20732
28961



17883
26207
15028
8829
17171
18002
13614
19852
15226
28575
13516
13519



1247
7513
15236
14803
23564
15975
23296
25872
28950
16711
29243
20096



26412
18845
10786
17335
9678
25604
13488
14149
3283
17045
21435
14219



12544
1057
11976
11975
12001
14156
13164
15233
28285
8658
14918
5200



2118
7434
23750
15810
28718
29857
17420
25851
8755


528:
16492
6409
2252
1593
19867
28929
30208
13118
21413
12266
21411
28238



9482
6938
27956
30367
4687
9664
18377
6766
6719
24296
17192
14935



16375
3675
14099
4268
530


529:
22898
22919
4748
22980
30423
15119
24626
932
10860
16283
12991
20127


530:
16492
6409
1593
19867
2252
28929
30208
13118
21413
12266
21411
28238



9482
6938
27956
30367
4687
9664
18377
6766
6719
24296
17192
14935



16375
3675
14099
4268
528
24265


531:
1675
6747
9024
20653
21604
15192
16195
30132
8696
9651
10734
18036



10373
7230
20184
7055
14708
2071
16818
9305
18261
10313
9104
10846



15498
10811
10836
6197
6676
6675
6748
28327
3822
25411
22070
27551



26669
30270
5153
20247
2823
10651
21365
24415
20606
13317
16566
22225



18828
21467
18664
6674
21325
6680
18734
8153
27563
25667
29627
4344



29742
3648
2841
26738
11273
11248
15844
13246
13282
22458
8466
29937



19600
16194
29276
21189
24558
24568
23654
20112
30156
26176
954
18522



24967
25531
16036
27834
19239
6710
26317
18659
13733
12973
20870
19262



24907
26091
27855
27872
25795
15417
4882
22678
14382
30339
2155
18657



6681
6685
17903
17894
17898
28166
1755
3114
30029
6714
30415
30416



30136
3010
14351
14347
18663
3085
26884
25919
25627
27129
23585
16697



24254
22311
1805
4618
29326
12209
8145
7712
13051
18626
27207
15402



28900
9215
17833
26035
19110
8394
25682
1973
17372
19332
7535
9804



4896
8040
10235
10407
29660
22707
8266
22906
23726
14107
11376
5068



3753
13316
13653
5032
25221
2310
6694
23479
23270
24930
20214
20704



17506
12834
7512
21667
27720
2463
3970
1738
18275
19022
24566
18747



12306
22142
15316
12971
6069
9071
16992
7489
3063
21740
1400
29941



7403
6464
11415
16092
4254
1222
6822
1684
1720
18021
8323
22224


532:
7364
29443
860
12274
4596
25725
30280
12581
7597
26675
12353
16673



13436
1092
5023
22887
1097
24622
7524
3315
14207
8563
22909
9610



25598
14994
3885
19801
9273
11933
12775
20422


533:
28376
13430
9755
29312
6924
16479
18829
21115
11828
13457
24688
16663



22717
10343
15684
28381
23673
17085
21309
24356
28514
25283
4923
22990



8490
8880
10422
12749
18007
5763
8581
21433
5689
21120
16299
26580



1685
20921
29093


534:
11865
5762
26893
29386
28054
28057
28051
28053
19402
18298
18321
20379



30260
20170
26373
28700
28701
18746
11370
11286
11412
16021
15038
9660



9697
9721
9693
12599
24997
21866
21417
2876
21418
15790
21869
30165



12850
12849
12856
12848
12853
10934
18040
12715
8243
1096
18754
11923



5388
6595
21842
14955
12331
20861
14547
23644
26004
2001
11490
14934



6066
6208
3375
6663
15126
15715
28092
14463
17166
2313
20148


535:
14286
3849
11781
26893
29386
28054
28057
28053
28051
19402
18298
18321



21864
1838
23932
30158
20379
30260
20170
26373
28700
28701
18746
11370



11412
11286
9721
9697
9693
9660
15038
12599
21866
21417
2876
21418



15790
21869
3120
30165
12850
12849
12856
12848
12853
8243
1096
18754



12715
5388
18040
11923
10934
6595
21842
21109
14955
12331
20861
14547



23644
26004
2001
11490
14934
6066
6208
3375
6663


536:
11584
13724
9554
16616
11861
29446
2685
2666
2692
1388
2697
1585



14288
1255
23869
7127
24502
19874
27071
12831
23261
4734
874
23045



23769
7502
28061
18457
15556
15559
15561
15555
15529
21912
15390
8535



8958
22252
24498
13117
25178
30459
15583
4752
23038
18254
7244
10345



20327
23212
5007
30091
7554
22684
5719
606
20333
15301
14483
792



29861
15027
18648
7016
13651
1770
16899
16855
28048
662
18404
15455



14827
22321
11701
24050
10538
15416
15419
15425
15460
15427
15380
15459



2809
13727
17094
9390
1327
6658
9556
6697
577


537:
27487
26588
15322
6441
22352
19102
27509
1210
26463
25898
5441
27945


538:
25121
21384
7121
8414
12357
27953
13398
29440
4857
16974
11487
18184



3613
539


539:
25121
21384
7121
8414
12357
27953
13398
29440
4857
16974
11487
18184



3613
538
11807


540:
26182
23506
4721
3407
5510
28141
20289
2375
19707
8478
20026
20281



24204
25568
13565
7885
9747
12804
30409
27044
26268
3082
26845
25021



13039
21422
20711
14460
28379
6369
22171
9400
14912
802
24802
24771



8224
1502
13255
9710
8498
15184
15051
4279
14087
11687
5051
14366



16922
10833
5999
9792
11044
25909
13239
13275
30135
20448
13220
26919



4711
23144
24827
20648
21039
13567
26113
26111
28444
1311
29092
4556



15492
23079
2536
5889
23731
14554
14550
17606
16023
2106
26804
29756



4313
8632
7678
5806
20421
2398
2397
27397
23276
27338
4768
5609



3677
11920
4686
13177
8526
24531
24738
13805
8112
29518
21930
11695



22198
26120
22534
6274
29542
11876
14833
10972
22036
12767
3160
19374



20342
23781
23785
20993
27499
15470
9818
18102
9948
9496
26547
4383



10770
14240
9152
7152
7188
9925
6382
23444
17250
21693
8186
13000



11279
5403
10662
27406
30083
10558
28370
25205
7637
14097
14998
13965



21638
22371
1454
29332
16216
10914
19058
10891
21898
21990
7195
23665



28500
30320
29260
2161
28836
28872
18217
7183
21782
4672
7865
2807



6422
11110
17642
19531
8184
6205
26438
28401
5989
22023
25740
30012



7495
20566
6287
29783
25646
28764
28507
28368
12876
28396
26416
4190



28307
12312
29953
26577
23693
8130
24972
23063
26305
26248
30064
12578



4576
2528
24869
15032
27371
12697
11650
26296
12987
1871
19446
25420



2484
29690
19648
14582
23482
17442
23413
23216
3489
16477
3378
10348



6138
26364
4192
8154
11757
7232
23920
1542
22099
5372
5373
12265



1068
4102
21878
21348
19892
19977
21412
16436
16433
20230
8918
8920



22210
5330
9026
10213
8952
9259
29706
13303
21389
10071
13931
8560



14982
10498
10495
17994
5401
16858
8768
9509
20814
17937
7058
22326



29480
21749
14578
14549
29370
27230
24836
18516
2993
23324
5787
22836



5723
8582
7719
27248
1568
19599
2387
23790
26678
22983
7013
29168



15058
1150
27458
4374
29230
29228
6520
21564
9671
11335
11057
11637



24752
23041
3411
29513
21057
21024
17434
19365
16579
10330
29074
24593



6689
17303
25838
22875
14716
13596
19206
14325
21526
28194
1382
2941



1109
23948
7856
7884
7993
5686
5875
12179
10478
23287
18636
18621



14476
14438
11410
22059
5037
18708
6815
6411
4820
17808
4072
23969



27659
1547
12788
29619
26791
9760
4675
23624
23629
14213
22376
21266



8617
8615
2769
4406
29450
23238
23234
17190
19530
29629
19814
18473



19369
28542
4823
19881
20715
19875
16404
15253
14816
14131
7741
23111



16329
26383
5163
5886
12961
15592
27761
19879
22640
7217
14628
1248



18929
16814
26863
24767
2595
2583
6079
22291
22294
25697
765
1356



15204
12895
28219
11277
18540
28892
11025
2158
1919
2932
12172
23725



22155
5485
9908
10329
7268
7386
5378
11875
20489
16154
26344
1991



15586
12459
9866
5442
12096
26240
19959
20139


541:
22243
22240
26949
26774
29683
23772
17552
29740
9778
2873
22325
7529



19007
11351
13667
14798
14772
16896
17729
8513
7501
26528
25615
24929



15979
15977
26249
6954
14735
22375
2091
2095
27749
12131
24367
23704



17863
19615
20629
12939
17501
16781
19684
21815
12638
12466
17498
21314



7505
7500
7482
7478
7472
7509
21278
20224
9137
4082
20639
18719



18718
18716
9036
3732
10733
18116
2287
8712
24065
21209
12250
19361



1405
7693
3342
8585
14052
18176
13379
26334
17183
27452
21264
25368



26540
22791
18496
7202
16662
7709
8412
4184
3904
7099
24703
16027



27455
27486
27374
26793
22065
27056
29553
10267
28673
4312
28128
23355



25447
22549
30363
3300
16696
8688
7382
16950
1795
4152
22188
2790



3302
23083
18446
1610
19171
10417
14619
1067
10645
10299
22958
16083



16622
22863
27719
29767
19818
20006
27261
27241
11614
11588
11647
27762



22120
14245
1071
12970
18409
27276
6326
2447
14057
17251
23173
5268



5272
7657
8298
7780
3599
15364
29591
29503
17641
22582
18858
28395



20159
12662
19081
16368
23618
11114
15437
1679
4012
6344
23108
23085



23082
23080
23103
14066
7803
24918
15433
9008
6879
19641
15435
19032



11136
3314
19051
25287
18258
1814
15532
15536
15802
23603
24372
24211



15438
15430
11291
1865
7570
28732
632
15426
14712
14711
14713
11429



18952
24537
12400
12462
9297
9294
798
30272
30269
15758
28047
3077



22570
27250
6443
8290
2926
11586
20772
20776
20775
25367
7673
7659



11307
7710
7715
7708
6014
5973
5970
5967
5964
20151
6021
5977



14752
9944
29498
23222
23211
23254
22474
23367
16955
16753
6873
28254



20645
22646
25158
23132
23135
5694
27562
12106
21555
5221
27356
27354



27358
27360
27383
9917
2241
11710
15476


542:
20858
10809
26142
14612
16454
19545
19537
19540
8482
24371
4160
20283



14553
6799
25202
9659
14445
24224
27194
8364
19737
19738
8866
7937



25944
25059
10155
21478
7852
7102
14451
6950
5026
14406
28090
5094



21421
13411
27424
6366
14790
22169
26988
24801
2483
13224
14147
14409



14494
14517
14518
14520
14521
7173
19631
19633
29864
19669
19674
22743



4381
12661
8374
12058
12037
27740
24959
11750
14092
11686
12278
12798



4989
7115
11906
25912
26228
24830
26620
20613
23068
23086
1638
4563



4496
15519
1197
14710
5891
26941
26940
26894
26897
26904
26938
26888



26891
4320
28614
2396
15349
5649
3734
19586
7900
2208
22821
15320



29934
11345
22535
1531
21396
14482
8469
29548
28635
9987
2543
21166



10952
12764
20540
23787
21038
27498
15465
17855
5288
27678
14444
14239



19778
7222
9980
3241
6735
17248
8355
5396
7572
4642
26594
30266



12513
15663
1676
1570
13712
25028
25204
15030
8967
9847
17346
22369



24433
3228
1492
21919
16560
18490
3457
29822
16356
20957
8669
27349



8556
22593
19181
14071
2338
26913
23223
19740
19741
19702
6424
12427



19528
28651
26436
23695
9106
18680
25651
29778
3385
25547
23653
16465



29957
23660
28119
25123
23161
10341
10337
17877
16926
6313
5335
27126



12985
8913
19166
26170
15524
3225
17431
25803
19232
22221
21808
25987



3306
21033
12951
5949
12101
11207
26677
5377
3154
10277
4237
10464



21397
26222
24026
22976
16432
14172
24505
8923
20766
7372
30063
22214



27929
8234
29711
2824
2892
13307
1027
2055
10073
17104
8541
15113



10000
24419
9110
10493
28628
22247
5404
29270
16863
3428
28997
21506



14549
25281
28269
28867
11006
17838
27771
12933
20692
19733
19704
27251



2386
19598
10663
23428
2637
27711
19997
18774
25727
27718
9402
6666



24817
13556
9647
5248
11562
13064
16054
20698
15992
23836
26766
12367



9376
25609
21567
12837
15575
19584
14844
25156
21026
3140
24917
25275



17394
24748
18515
8565
28120
13593
4610
26924
13573
23412
6478
10079



7860
12653
7702
19415
11774
6562
10481
11804
6051
18624
18909
8442



12311
22061
17975
11180
17334
3404
6618
3733
12950
9766
5493
3980



23627
23968
14205
26388
16017
19226
30230
22394
8620
15956
19582
19549



19585
29807
19635
19638
19666
29483
23233
375
21589
4151
18853
16248



21296
24366
16526
10967
7110
2896
26390
27580
28419
16446
16451
17612



28143
14479
8885
17298
5845
14415
14487
14465
14491
13609
14489
14378



13581
14371
13583
14374
13616
13608
13572
14389
13612
14384
13577
13619



14449
13570
14523
21910
14411
16057
19767
23470
15894
16817
25786
2582



28328
20366
22975
16439
22295
21402
19862
28494
14459
1199
24733
4349



14073
5733
23439
4372
13627
8409
28221
27151
26080
26055
25984
25982



26010
25976
26083
26822
26852
25978
26017
25959
26132
26133
26850
26859



26820
26825
26106
26109
26105
26022
26026
26827
25956
26085
25981
26135



26131
26104
26102
26076
26057
26015
26159
26818
26156
26053
26054
26161



26060
26136
26163
26160
20849
20846
25951
25955
20885
20842
26078
20891



20886
20850
20808
20838
20718
19833
20686
21980
19803
19799
20687
19794



19772
19797
21983
19770
21981
19842
19839
21960
20689
20719
19832
19805



21976
16489
16456
21012
20804
20767
20754
16486
20973
20970
21908
20969



21880
21010
20759
20930
20895
20923
21879
20966
21847
21911
21044
21948



21838
21004
20972
21882
21844
21050
21055
21014
21003
21843
21916
16491



21957
21915
20893
20723
20796
21845
21953
21877
21946
20721
21048
20927



20925
26854
20926
21884
26858
21828
21803
2162
1916
5079
2116
14186



4604
4553
4521
13195
24012
20562
18518
6546
957
13095
2878
25070



5498
20329
27871
15849


543:
15285
20482
26613
13010
14587
23348
17517
1915
30335
17540
18023
13902



2215
19384
5020
18425
13592
17005
27801
12620
13102
14308
12370
25212



18833
13754
16338
24536
17029
28412
23157
6821
12613
5346
28829
3443



14707
14529
24381
10928
26280
16516


544:
4536
25496
28709
6661
25923
19605
8730
26387
24734
27819
26391
24458



1276
27415
13295
21712
2654
9901
28582
3974
9593
12761
5972
17010



17012
18764
18766
26843
11762
5896
25057
22202
7424
8694
18683
12734



28761
13476
15235
18427
27902
26887


545:
12056
13356
8152
11079
6304
8906
15970
12431
25131
7138
7325
29624



4272
19148
22274
20301
11681
23992
21196
20167
12503
12758
20453
21517



9990
20365
8227
12406
24019
2884
1943
26426
21282
16574
10447
28071



23258
25792
23010
4308
8045
16351
6883
6696
15209
3194
8198
15469



16547
30383
5262
29564
30255
25461
17243
27291
13943
23751
21208
26238



8332
27345
22817
17321
27526
4145
25049
23169
8088
26273
28856
8990



22046
4267
8099
8700
6867
3156
17467
29726
11140
24478
14750
625



6451
8709
28679
28038
28001
24064
14675
20054
7000
3451
6116
6082



6076
22932
15566
15563
21665
21666
11642
12287
6557
3864
2918
21850



3189
20742
3187
19334
26824
996
19103
19430
8381
12625
16081
16825



6972
12801
26676
22290
12917
21013
9589
24625
29608
6071
23377
23980



12412
1989
27630
28363
11724
4814
23346
25177
831
16951


546:
16231
8527
24427
23271
23243
25551
18288
7869
11697
11141
13630
14901



28300
12403
15034
2921
27414
5640
3475
28127
22446
21874
21875
26216



17530
27795
8524
2321
8493
8530
8494
23701
30045
30432
27595


547:
22240
22243
26774
29683
26949
9732
16548
17552
29740
24038
7529
11351



19007
14772
14798
27139
30265
17691
26528
25615
1769
7501
15977
24929



15979
26249
6954
6081
4811
22375
2091
2095
17501
19593
16053
9137



4082
10703
20639
18719
18718
3732
10733
9036
8712
2287
26795
21209



3361
19361
7693
3342
18176
13379
23433
27452
16662
9601
4184
28719



14891
23285
27401
27056
21680
28083
30363
3300
20716
10231
17069
29023



1610
19171
10417
14619
27669
29767
27261
27241
27762
22120
12970
1071



14245
2874
6019
5268
5272
7545
27088
28278
14365
12561
28395
20159



12662
19081
20880
16368
23618
11114
8208
29667
18517
21904
4520
18090



20800
5908
4172
16285
6944
14066
25462
6898
5734
5737
19034
15089



6828
14820
10334
5790
5254
5014
6836
6840
10524
11295
28438
6345



11461
12400
12462
9297
9294
798
30276
30279
3639
10399
2937
20776



20775
25367
20772
7673
7659
14752
29498
23222
23211
23254
22474
16955



28254
25158
23132
23135
5694
12106
27356
27354
27358
27383
27360
7564



2258


548:
26090
24906
7328
16079
13640
1998
3987
24547
10118
10168
19534
29751



23972
19048
19518
16929
12836
10980
5385
12514
8603
21615
14006
9621



29755
3278
9131
13564
783
18177
14122
16367
18197
10484
27976
27790



10726
28360
7845
8361
7214
3978
10445
26641
12573
11417
1937
8810



18305
17353
26604
11764
14422
27199
13341
7149
27631
850
24218
22409



8455
30360
3313
24294
14091
12152
23301
604
28356
12273
17439
5642



24682
21304
19627
21743
5612
8426
27392
19043
29067
5266
8170
5679



6456
10383
22771
8450
17154
17655
21229
10695
22850
20714
3703
17981



27996
24666
29419
13898
25251
2927
968
25866
11823
11437
11556
29559



28036
22558
28576
18030
644
20487
13123
6229
21755
4019
11073
14826



8138
4868
5476
13751
26764
24215
21989
8521
23457
22775
15037
29764



12464
17472
1453
3416
1990
16594
2573
20483
23359
4684
14747
21853



9108
9107
22028
22025
22021
24031
21991
26569
29011
5830
3328
15099



15130
21719
26252
13720
2076
11918
4664
8250
28676
18541
9328
2455



2456
2459
27366
27372
27370
13269
13267
9730
9757
9762
21183
21426



14657
11572
11571
15508
22496
21996
21999
995
14372
14890
14882
14886



14908
30442
13868
14633
10627
10628
10603
9996
18761
17083
26475
9727



28330
16618
2998
21992
24297
24316
13140
2970
22223
16540
763
16890



3910
16235
11545
28378
28077
2792
23797
2309
14104
7723
27167
10108



12589
10780
13561
10432
24018
30465
23865
12964
15841
26552
1195
25683



8249
30246
26838


549:
1877
11478
12729
25130
1968
1905
22481
10656
10659
29731
29801
27643



17021
714
21585
8258
9099
4230
29698
29696
26561
4191
1099
29827



29854
7534
3244
29821
29850
29824
29852
10968
10966
631
13526
5890



3446
6703
13508
3492
3495
10876
13337
6008
6006
6013
6015
6041



6018
25925
29829
29800
29831
8497
7953
26401
1095
1969
5740
11097



11671
12568
11672
13453
24895
12520
11494
11458
11498
15061
27293
7843



6148
6153
26487
878
9774
22442
22471
22472
22445
23110
22479
3053



3019
25794
3527
11473
25486
25512
10716
10694
10719
1101
21420
20389


550:
1877
11478
12729
25130
1968
1905
22481
10656
10659
29731
29801
27643



21585
8258
9099
4230
29698
29696
26561
4191
1099
29827
29854
7534



3244
29821
29850
29824
29852
10968
10966
631
13526
5890
3492
3446



6703
13508
3495
10876
13337
6008
6006
6015
6013
6041
6018
25925



29829
29800
29831
8497
7953
26401
1095
1969
5740
11097
11671
12568



11672
13453
24895
12520
11494
11458
11498
15061
27293
7843
6148
6153



26487
878
9774
22442
22471
22472
22445
23110
22479
3019
3053
25794



3527
11473
25486
25512
10716
10694
10719
1101
21420
20389


551:
12056
13356
8152
11079
24868
11081
6304
8906
15970
7699
12431
25131



7138
7325
29624
4272
19148
22274
20301
11681
9560
23992
21196
20167



12503
12758
12507
20453
21517
9990
20365
12406
8227
2884
1943
24019



26426
21282
16574
10447
28071
23258
23010
25792
4308
8045
6696
15209



3194
8198
15469
16547
30383
5262
30255
29564
17243
25461
27291
13943



23751
21208
12723
8360
27345
22817
17321
4145
27526
25049
23169
28856



8990
22046
4267
8099
17467
14750
24478
28679
8709
6451
625
14675



17465
17089
7006
14679
3759
24990
3451
6082
6116
10676
6078
22932



9844
15564
21687
23511
12287
2918
21850
3189
4533
9356
12014
7981



19327
26824
996
19103
7585
8381
12625
16081
25374
6972
12801
12917



9377
25188
1307
879
25200
17548
9926
21013
9589
24625
29608
6071



22139
25295
25185
25344
23377
23980
12412
1989
27630
28363
11724
4814



23346
25177
1342
27275
16879
6418
9242
4795
1399
12175
8327
8296


552:
19781
24742
27550
17602
13205
15958
18307
16764
3929
17477
21928
14400



21933
27006
916
18115
6993
25331
4503
4507
15940
682
25053
14192



24761
23031
20685
11089
7209
9758
10534
11206
4427
9159
17658
11938



3124
10347
12049
9558
15502
3366
27361
27355
6040
3911
9985
2893



9207
9103
9995
26691
14148
20147
20181
29859
27613
12782
12786
26910



16035
28693
10485
28340
27984
17004
14462
16386
17597
29867
27179
14346



18593
18567
11888
9191
6960
12451
28617
24925
1350
30251
12302
22551



22548
18216
21525
9960
28505
26680
18460
19054
26420
2184
28630
28660



15705
15724
8893
14296
27310
24443
15659
4093
6389
2478
26983
25010



13132
4295
2529
2495
25236
20124
2507
1152
28282
645
2825
1005



28518
19167
21466
7537
599
18087
16298
18089
24852
11775
11749
26204



22018
27920
16003
9786
18867
17703
19448
25939
21338
28627
2171
3726



12641
16435
11830
18711
9190
20376
26343
16247
3938
12903
9822
2412



23436
29142
28502
6323
13150
28551
27425
27016
2853
26310
23414
19425



9079
16055
5625
16085
16999
15000
23468
3658
3655
22468
11497
11504



2129
30286
25392
30355
10317
10421
15053
25299
16571
16554
12626
11521



27936
4845
9201
3312
6579
9500
17290
12355
10927
17798
16824
14684



20018
20105
26393
17519
16804
21988
16061
14242
29971
4816
23345
3015



2322
14182
7352
9049
9052
9075
28540
20892
24955
22823
27287
11526



4828
925
1159
1462
20485
29252
20515
28349
21472
10456
24784
20662



20700
17775
21939
7768
1374
1120
25719
29293
23096
22813
8592
15249



5391
1354
24887
10031
11029
20019
18824
18337
5546
7498
8616
28777



22506
24617
16583
24760
3188
7237
29337
23884
21937
21938
27713
23133



29373
3013
19023
25645
29183
5089
28861
8217
8794
29210
10457
6835



27792
1774
13788
18773
27759
22960
7483
3513
5800
28583
11996
8087



15913
4796
11215
8205
23783
24712
20834
22914
26209
15278
29766
5769



9871
11801
14191
4161
21690
21721
21725
9665
27460
17474
25268
22523



17600
21528
19363
8212
27682
14221
23190
24326
24128
9189
8795
23067



23908
23822
23843
23947
23882
23846
23844
23838
23817
23878
24021
23818



29053
27160
7596
30008
16281
24613
22747
24572
28587
20237
21376
7451



4727
6917
4763
21170
25724
9343
29782
6155
29792
598
3247
10391



3122
3052
9087
30207
16468
9873
20241
10159
16271
3867
13217
14330



15999
1849
28546
18193
6959
2022
2861
4341
4298
4336
12393
2559



7213
6281
6306


553:
26182
26171
26138
9717
23506
4721
3407
5510
28141
20289
2375
19707



8478
20026
20281
24204
25568
29196
28839
29900
5917
13565
7885
9747



12804
30409
27044
26268
3082
26845
25021
13039
21422
20711
14460
28379



6369
22171
9400
14912
8666
9592
9670
802
24802
24771
8224
1502



13255
9710
8498
15184
15051
4279
14087
11687
5051
14366
16922
10833



5999
9792
11044
25909
13239
12371
13275
30135
20448
13220
26919
4711



23144
24827
20648
21039
13567
26113
26111
28444
1311
29092
4556
15492



26276
23079
2536
5889
23731
14554
14550
17606
16023
2106
26804
29756



4313
8632
7678
5806
20421
2398
2397
27397
23276
27338
24306
4768



5609
3677
11920
4686
13177
8526
24531
24738
13805
8112
29518
21930



11695
22198
26120
22534
6274
29542
11876
14833
10972
22036
12767
3160



19374
20342
23781
23785
20993
27499
15470
9818
18102
9948
9496
26547



4383
10770
14240
9152
7152
7188
9925
6382
23444
17250
21693
8186



13000
11279
5403
10662
27406
30083
10558
28370
25205
7637
14097
14998



13965
21638
22371
1454
29332
16216
10914
19058
10891
21898
21990
7195



23665
28500
30320
29260
2161
28836
28872
18217
7183
21782
4672
7865



2807
6422
11110
17642
19531
8184
6205
26438
28401
5989
22023
25740



30012
7495
20566
6287
29783
25646
28764
28507
28368
12876
28396
26416



4190
28307
12312
29953
26577
23693
8130
24972
23063
26305
26248
30064



12578
12560
4324
4576
2528
24869
15032
27371
12697
11650
26296
12987



1871
19180
23167
27165
19446
25420
2484
29690
19648
14582
23482
17442



23413
23216
3489
16477
3378
10348
13228
6138
26364
4192
8154
11757



7232
23920
1542
22099
5372
5373
12265
1068
4102
21878
26956
30275



21348
19892
19977
21412
16436
16433
20230
8918
8920
22210
5330
9026



10213
8952
9259
29706
13303
21389
10071
13931
18857
8560
14982
10498



10495
17994
5401
16858
8768
9509
20814
17937
7058
22326
29480
21749



14578
14549
29370
27230
24836
18516
2993
23324
5787
22836
5723
8582



7719
27248
1568
19599
2387
23790
26678
22983
7013
29168
15058
1150



22530
17837
26771
27458
4374
29230
29228
6520
21564
9671
11335
11057



11637
23873
24752
23041
3411
29513
21057
21024
17434
19365
16579
10330



29074
24593
6689
17303
25838
22875
14716
13596
19206
14325
21526
28194



1382
2941
1109
23948
7856
7884
7993
5686
5875
12179
10478
23287



18636
18621
14476
14438
11410
22059
5037
18708
6815
6411
4820
17808



4072
23969
27659
1547
12788
29619
26791
9760
4675
23624
23629
14213



22376
21266
8617
8615
2769
25811
4406
29450
23238
23234
17190
19530



29629
19814
18473
19369
28542
4823
20640
19881
20715
19875
16404
15253



14816
14131
7741
23111
16329
26383
5163
5886
12961
15592
27761
19879



22640
7217
14628
1248
18929
16814
26863
24767
2595
2583
6079
22291



22294
25697
765
1356
15204
4401
20610
17157
7629
14311
9146
12895



28219
11277
18540
28892
11025
2158
1919
2932
12172
23725
22155
5485



9908
10329
7268
7386
5378
11875
20489
16154
26344
1991
15586
12459



9866
5442
12096
26240
19959
20139


554:
26708
26727
26707
12532
13832
28786
23554
3996
3961
3964
3949
23584



3950
23553
23574
23581
23573
23582
23580
3952
3994
3959
3946
3955



3948
28010
8799
18294
5808
17684
10249
768


555:
16550
16134
23003
20840
13449
2662
23631
14173
12029
1116
19485
27743



13656
7996
8349
6481
10989
9388
26088
12925
21513
2843
27683
5410



16798
26686
23450
6237
6341
27505
15031
5944
17208
3129
21831
7433



25746
23877
23430
16379
27758
4871
27605
4946
7706
24786
6179
23013



23502
22094
7336
9048
26957
2202
1754
29911
5726
3468
6786
24530



8257
3382
5016
21265
13625
4394
26084
27833
718
25361
27506
7591



3942
6693
10984
15234
25994
13459
27404
12200
17174
557
25015


556:
18237
23960
23994
23991
24179
24180
24076
24118
24146
24149
24030
24181



24184
24209
24210
24213
24217
24110
24232
24034
24105
24236
24238
23998



24071
23989
24241
23996
24178
24037
24027
19800
29453
11999
10858
12309



3763
5918
20729
17637
6952
6641
26191
9629
19472
26754
505
13319



6060
4273
7915
30110
23241
24337


557:
16550
16134
23003
20840
13449
2662
23631
14173
12029
1116
19485
27743



13656
7996
8349
6481
10989
9388
26088
12925
21513
2843
27683
5410



16798
26686
23450
6237
6341
27505
15031
5944
17208
3129
21831
7433



25746
23877
23430
16379
27758
4871
27605
4946
7706
24786
6179
23013



23502
22094
7336
9048
26957
2202
1754
29911
5726
3468
6786
24530



8257
3382
5016
21265
13625
4394
26084
27833
718
25361
27506
7591



3942
6693
10984
15234
25994
13459
27404
12200
17174
555
10258
25015


558:
11865
14286
1773
14694
9046
10926
2603
9799
5762
22591
8702
14134



19345
3849
11781
26893
29386
28051
28057
28054
28053
19402
18298
18321



21864
4487
11071
1036
30260
5652
6536
28700
28701
18746
11412
11370



11286
9697
9721
9693
24997
9660
15038
12599
22363
19623
21866
21417



21418
2876
15790
21869
3120
30165
9567
12850
12849
12856
12848
11923



1096
8243
12715
18754
5388
18040
6595
11983
21842
10934
26685
14329



26134
7114
6547
13437
14955
12331
20861
2706
14547
15411
22324
19562



21801
7211
20912
23644
19326
26004
2001
11490
14934
6208
3375
6663



12294


559:
21053
5637
13367
30097
30098
1714
4293
13481
14355
1206
1181
7897



14745
9179
2803
2806
19460
10635
28838
25830
16687
19459
23427
9319



15848
15846
12390
12391
10033
21274
8353
18944
2176
14748
9271
11074



12439
7936
20697
13928
20737
15591
1381
1363
5045
5043
23810
15873



18769
26971
26972
9219
3214
3212
2831
2833
16064
6039
24993
24991



27439
27438
29207
1634
19383
24416
24418
16903
11802
13429
2494
28555



28557
4328
3269
3272
14997
1597
27111
25193
9655
13046
13048
6270



7198
27215
1146
26515
26517
21921
15283
27402
27868
9639
16209
8679



15709
27332
20494
13930
5116
6353
7756
7601
8986
9515
1362
1365



685
24332
25918
25928
20497
12076
973
974
7589
1314
2849
7200



21813
9155
9156
10841
19587
19589
6171
8235
16570
3531
3526
8286



3377
5657
22868
22870
1551
1553
26201
26375
6394
14686
14687
26817



2903
10287
16363
11145
5246
5250
14737
23734
23730
21861
21859
26687



29894
30434
30431
2899
2902
16334
5302
5298
9888
4303
10265
22367



18580
14695
27846
17626
2109
6052
16127
30216
4367
6687
26267
24728



24730
14722
14720
1445
27700
27697
1322
18938
17173
17172
14492
24191



26309
26311
18431
18429
22637
22639
1746
841
19542
25007
23073
921



8727
22508
11259
2295
28478
17819
26866
29146
1507
7960
7961
11492



26369
26371
18436
5241
24911
10101
10102
7319
7323
10634
18983
29268



29265
26149
26150
21925
21927
17972
18001
20748
20747
23042
30325
19845



24599
4808
29229
14641
14643
6370
15708
15710
29633
29632
21113
21114



11209
11213
22426
22424
29203
29204
29502
29488
4378
26546
26549
4062



18589
26724
6332
23821
27135
22196
16034
11065
11709
23015
18600
2580



29261
27119
27964
27971
11480
11481
9572
17430
17428
9575
7945
14729



8825
26717
26720
9302
9326
18620
924
27200
20233
10699
27257
26880



26881
12995
17825
17823
24741
22069
19432
19359
13970
3496
1711
23383



21158
12866
30263
18787
26538
14868
4035
4388
10801
10632
25632
23963



28612
23253
27927
1178
29132
20213
1016
14461
24758
23386
21388
5313



5058
28289
4759
4761
4762
2437
12185
12187
18988
1713
28792
18380



15839
14194
18840
4330
4864
8121
9335
10310
10638
18392
21281
20942



20443
26148
20072
21300
12484
2275
25950
3671
29507
16628
10059
2250



1723
882
29614
22890
7292
3148
16143
24227
8787
10741
1326
5613



9289
9306
865
6419
4798
13777
13774
17747
17712
708
16833
3004



17056
6800
2653
20042
17162
26198
26200
27852
24966
24968
11426
30306



30308
5191
6541
20215
20217
1772
3268
4046
4053
4050
3207
3027



3022
4057
3074
3029
3173
3168
12543
10664
21634
17128
6401
12365



8519
13988
13986
30242
15429
9261
23736
7773
12871
20211
20212
2210



10272
16332
16353
5091
25709
22283
592
2498
2518
3861
19815
28848



16627
1790
20457


560:
16185
25895
4001
30373
8413
24865
7113
11532
12890
18385
12053
4807



8506
17850
16626
27185
8569
12416
19408


561:
23793
18790
29098
21734
13819
24140
18727
8559
785
19511
16337
27278



1226
20337
1398
2189
29255
9913
972
8849
20168
11234
17738
9477



28987
15076
25762
8410
23602
11310
9650
4780
13397
24565
16656
23229



26118
24815
15206
19896
6288
13671
896
30168
26836
24841
24102
16849



3941
562
13157
14350
16990
10451
11525
26264
29121
8019
18656
20809



26496
13454
15045
4528
20271
17831
600
24452
12828
6707
26769
638



13386
29429
20627
20839
28624
12407
3134
16709
563
637
24275
24542



7836
27684
13611
18144
29774
12757
29174
5497
7397
25897
19074
5958



4432
4647
10526
5714
20064
13499
9446
27604
19261
27314
23489
15876



20588
1701
10200
26101
8656
7051
11891
7136
5827
18060
8242
12109



21489
8385
29785
8389
3731
29847
20504
29916
16445
11086
24284
14179



24668
24107
24106
9456
4469
9743
675
3153
4103
1313
26756
18494



14533
16275
15083
17043
3645
28897
6403
7837
23029
11293
20956
12145



14434
25871
5971
19784
17500
22386
28165
13955
8924
16793
22370
12281



24468
2821
3905
17904
9915
22744
26446
14856
11630
21379
24611
13726



20506
3830
25891
29537
9372
11185
18947
18702
19469
6365
1988
28035



1228
7358
3014
1690
2393
26723
26830
22513
775
2282
13540
770



23142
15615
27092
23883
922
24454
20351
2725
12230
11676
2815
9204



18941
4116
27727
16147
15624
24212
6251
4480
13344
23040
24055
19070



21441
5663
25714
30134
1184
24989
4728
29452
22470
26402
11398
11262



12130
2044
12505
11829
8671
20459
14527
7871
2587
26994
15300
18248



2979
14490
1808
8094
9346
22495
17804
2722
4594
27317
27593
23670



28675
12119
15392
17875
7057
13063
23126
23579
20651
27745
27584
26925



20778
1598
14158
14079


562:
23793
18790
29098
29530
21734
13819
24140
18727
8559
785
19511
16337



27278
1226
20337
1398
2189
29255
9913
972
8849
20168
11234
17738



9477
28987
15076
25762
8410
23602
11310
9650
4780
13397
24565
16656



23229
26118
24815
15206
19896
13671
6288
896
30168
26836
24841
24102



16849
26769
12828
638
16709
24275
637
563
27684
7836
24542
18144



12757
18060
8242
12109
21489
8385
8389
29785
29847
3731
20504
29916



16445
11086
24284
14179
24668
24107
24106
9456
4469
9743
26756
18494



14533
16275
17500
13955
28165
8924
22386
22370
2821
16793
19784
17904



26446
9915
14856
15615
27092
922
23883
24454
20351
18941
12230
2725



9204
11676
4116
27727
16147
15624
24212
23579
20651
561
28151
9630



27745
27584
26925
20778
1598
14158
14079
14350


563:
23793
18790
29530
13819
24140
18727
8559
785
19511
27278
1226
20337



1398
2189
29255
9913
972
8849
11234
9477
28987
15076
25762
8410



23602
11310
9650
4780
13397
24565
16656
23229
26118
15206
19896
896



30168
26836
24841
24102
16849
16990
10451
11525
562
13157
14350
26264



3941
29121
18656
26496
29429
13386
6707
20627
12828
26769
638
20839



28624
18060
12109
21489
8385
8389
29785
3731
29847
20504
29916
11086



16445
24284
14179
24668
24107
24106
9456
4469
9743
26756
18494
14533



16275
1313
20351
23579
561
28151
27584
26925
20778
1598
14158
14079



24275
637
16709


564:
25129
27368
25249
29970
4685
4766
10275
29055
21313
10701
19680
9935



16488
8177
4015
27951
25825
21512
20368
27128
9690
12146
27413
15195



23828
11841
5189
14776
9064
21233
26231
12002
11133
9018
15501
26127



12008
11129
7116
16009
27954
10924
12108
21081
12026
22330
27916
24283



7140
27779
24073
4136
30112
11168
1115
22933
22672
19676
21195
1111



11985
28917
22696
16189
13287
3293
12420
30163
6141
3686
20573
16193



23458
4617
4625
24189
11578
9003
13450
11577
11574
12862
21165
16191



11772
16870
29589
7318
7287
7322
27518
12423
6771
29765
18959
18928



6767
18139
771
6718
10234
14176
7640
29596
7371
15272
14212
10238



5355
23723
23465
10565
7485
1227
22251
16546
24889
29958
25556
5655



8828
28823
28380
2635
12575
21856
6971
17870
15410
13234
11015
22504



8340
8342
9158
18685
29258
8072
29470
19267
18350
2117
14539
725



3730
13289
14559
10188
15006
7581
22282
5390
2261
10220
2021
22170



22174
22172
16620
6756
12246
18575
17265
21720
17791
3742
25238
7440



19956
1764
19482
23779


565:
13444
29969
14565
8761
4609
27488
15129
21484
10063
3692
15266
13584



9606
3133
29198
12289
29118
4198
3469
1246
9029
22236


566:
12946
14206
6593
6591
25758
19731
19754
23642


567:
13038
4724
16883
8660
7271
2574
7312
7307
19099
16463
22752
16786



24534
1935
18448
8772
9405
12044
16340
27473


568:
20417
27886
19619
7800
1272
3823
7082
27564
15616
24116
14223
27721



8014
22783
8325
24621
17123
20009
29665
28581
24641
24616
24828
13057



16913
9997
29744
10107
29748
29746
22723
14234
2110
8575
10591
9773



9777
8670
11915
18424
9511
18564
20764
13787
18423
11187
18604
3516



20154
14600
14603
19437
26638
27689
27696
27693
22261
12113
10921
9824



7009
7004
8973
8978
6447
1337
1352
16043
21920
21961
8161
8211



26300
13172
13843
20552
12458
14142
9797
19787
3606
25773
16317
23816



3673
899
13278
22264
22235
22260
22267
17224
14236
10840
11111
11109



11107
19732
3976
5933
6842
28949
20695
9231
2267
22271
9587
27307



19107
21070
15001
21236
14427
27038
20250
12766
12763
6036
12377
9763



12793
587
14396


569:
1581
16146
11925
5139
11665
14692
30172


570:
1205
1202
23043
18478
21335
24048
24042
12899
18378
18376
6551
9413



9412
19940
19978
19981
2128
18413
18415
16198
16668
3185
15855
9352



14616
2960
2967
2966
4462
25905
11227
28186
984
13539
20758
20789



20750
20753
20749
25229
28534
17690
22452
24103
19988
21788
25087
19912



21275
19982
19909
24881
24899
26679
18852
27639
12055
16381
1897
1894



1892
20060
7377
10185
12191
13610
6686
27612
27664
3919
27641
10110



28531
28470
2758
4227
10669
8133
24960
23679
11153
19321
30078
21051



17845
17557
1936
23577
27609
28429
27692
28464
27698
27667
28432
28424



27637
19872
26730
18303
18438
18412
10396
9203
29169
12043
1965
24374



25660
17724
16729
20884
2141
19907
22922
27089
2063
26239
19936
19119



21272
19915
19985
19916
18368
18306
18331
18333
18338
17515
18481
17665



16401
13021
427
23518
7850


571:
15818
24500
6249
24365
21416
9058
5044
3647
14963
14493
20694
7254



20765


572:
28807
27051
3150
20358
5777
13209
5111
9645
17113
16565
22552
16503



25832
29107
1460
14728
21817
17382
20312
10501
20673
3346
1922
10077



6744
26143
7881
8571
364
26287
15280
26997
9989
27429
4294
26013



754
22726
25117


573:
8315
18414
30224
15804
2638
19500
17309
12992
30378
9019
1704
6981



7658
9420
23401


574:
19688
10946
17787
18811
24157
19726
16146
16140
13361
26217
825
17239



11925
5139
1815
428
13932
5348
14692
376
9975
30172
569
19165



576
29573


575:
4786
9635
22533
22517
6361
6393
742
9918
3287
10618
13765
22118



15728
12502
26571
17426
25481
6770
9626
2081
11885
24724
2078
13738



28822
8819


576:
19688
10946
17787
18811
24157
19726
16146
16140
13361
26217
825
17239



11925
5139
1815
428
13932
5348
14692
376
9975
30172
569
19165



574
29573


577:
11584
13724
29524
9554
16616
11861
29446
29504
29601
2685
2666
2692



1388
2697
1585
14288
1255
23869
7127
24502
19874
27071
12831
23261



4734
874
23045
23769
7502
28061
18457
15556
15559
15561
15555
15529



21912
15390
8535
8958
22252
24498
13117
25178
30459
15583
4752
23038



18254
7244
10345
20327
23212
5007
30091
7554
22684
5719
606
15301



14483
792
29861
15027
18648
7016
13651
1770
16899
16855
28048
662



18404
15455
14827
22321
11701
24050
10538
15416
15419
15425
15460
15427



15380
15459
2809
13727
17094
9390
1327
6658
9556
6697


578:
11761
19089
16165
8711
2759
12674
13415
29879
7066


579:
22555
27884
11448
8273
27880
8275
11446
27878
27874
3478
15805
11443



23102
23280
14976
1219
13309
22763


580:
7938
7964
1841
14824
14760
24052
3138
28102
21222
21218
21226
13120



4265
5711
11048
27430
17350
25395
9698
14345
25391
19114
11354
25539



2802
28086
19052
1394
4583
3706
3705
5387
4585
14414
5998
1847



27344
16676
16678
22469
24958
7905
7870
3660
12098
9513
6150


581:
12041
23443
2888
28895
11471
11483
11474
21745
12751
8116
16160
16162



17998
4000
27970
9384
5381
3441
24737
859
26362
24708
6811
6754



18178
22419
15989
15143
14642
1282
27419
18937
18587
23984
7343
15826



15863
17425
1009
8802
9588
7086
17485
1240
10670
10668
20548
27277



9613
19477
15631
11051
11046
11023
10988
10990
11021
11017
10994
10992



11019
11052
11014
9124
29772
24903
30361
12677
12681
17414
12679
21760



22304
26366
7605
27534
6568


582:
19546
7100
21078
29472
26206
14606
8808
9347
21542
10893
23028
28069



9435
27011
4314
15686
20175
18297
20143
6089
6122
27189
27017
26037



25820
24689
6779
23568
2628
19337
27084
30085
8185
3032
16480
5557



2421
18364
24072
19813
12996
10951
13094
7636
15739
29267
10609
16613



16763
26659
2575
17702
15594
12665
29225
8278
9180
28016
29556
25090



24005
9907
28287
24942
24941
5491
2270
16971
5215
5054
5062
5059



16969
22674
10175
10170
10172
10072
29929
10173
20423
14928
14922
8299



8043
26409
7503
3588
26584
28658
1029
12685
28830
25587
9165
17300



30326
23962
7436
12369
23509
16214
8176
29489
18480
2148
4847
19133



516
24058
10954
800
11217


583:
25129
27368
25249
29970
4685
30235
4766
10275
29055
24740
20607
21313



10701
19680
16488
9935
4015
8177
27951
25825
21512
20368
27128
9690



4877
27413
15195
23828
20916
28202
14776
9064
21233
26231
20077
25077



12008
9018
15501
12108
12002
11133
16009
26127
12026
11129
28917
7116



30112
24073
16189
22672
22330
10924
27779
27954
1115
22933
1111
21195



27916
24283
21081
30163
11168
19676
11985
4136
22696
7140
13287
24189



3293
11578
16193
20573
23458
4617
4625
9003
3686
12420
6141
13450



11577
11574
12862
11772
16870
29589
7287
7318
7322
27518
12423
6771



29765
18959
771
6718
10234
29596
14176
7371
7640
14212
15272
23723



10238
23465
1227
22251
16546
24889
29958
28823
8828
5655
25556
28380



2635
12575
6971
15410
21856
22504
8340
18685
29258
9158
8072
29470



18350
19267
2117
14539
725
3730
14559
10188
13289
15006
7581
5390



22282
2261
10220
2021
22170
22172
22174
16620
13494
14407
21902
17265



6756
12246
18575
21720
17791
3742
25238
7440
19956
4913
1764
19482



23676
564
28187
21453
11680


584:
2555
25500
14030
22659
25613
8260
27377
29793
1177
23381
14036
15968



21609
6826
5584
2182
28639
5929
1090
9967
16653
19423
6909
27061



805
16595
11380
27033


585:
16495
15818
18613
27890
10799
11700
27624
27254
9740
5044
6485
14963



14493
4453
8639
8663
8638
8637
9491
30129
23337
20765
19015
16487


586:
7759
1528
23333
853
16894
13376
11592
18242
15920
8992


587:
20417
27886
19619
7800
1272
3823
7082
27564
15616
24116
14223
27721



8014
22783
8325
24621
17123
20009
29665
28581
24641
24616
24828
13057



16913
9997
29744
10107
29748
29746
22723
14234
2110
8575
10591
9773



9777
8670
11915
18424
9511
18564
20764
13787
18423
11187
18604
3516



20154
14600
14603
19437
26638
27689
27696
27693
22261
12113
10921
9824



7009
7004
8973
8978
6447
1337
1352
16043
21920
21961
8161
8211



26300
13172
13843
20552
12458
14142
9797
19787
3606
25773
16317
23816



3673
899
13278
22264
22235
22260
22267
17224
14236
10840
11111
11109



11107
19732
3976
5933
6842
28949
20695
9231
2267
22271
9587
27307



19107
21070
15001
21236
14427
27038
20250
12766
12763
6036
12377
27914



9763
12793
568


588:
26090
24906
7328
16079
13640
1998
3987
14369
10118
24547
10168
19534



14201
4351
29751
23972
19048
19518
16929
12836
10980
5385
12514
8603



21615
14006
9621
29755
3278
9131
13564
783
18177
14122
16367
23248



18197
10484
27976
27790
10726
28360
7845
8361
7541
3978
10445
26641



1937
8810
18305
17353
26604
14422
26491
13341
8502
7149
14121
22354



27631
850
24218
22409
8455
30360
24294
14091
30220
23301
12792
17439



5642
24682
2394
781
21304
19627
7391
21743
2420
8426
27392
19043



22515
29067
5266
5679
8170
6456
10383
8388
22771
8450
17154
17655



21229
10695
22850
20714
3703
27996
24666
29419
13898
25251
2927
968



25866
11823
11437
11556
29559
28036
22558
28576
18030
644
20487
13123



21755
11073
8138
4868
5476
24215
8521
23457
22084
29764
12464
14383



27005
11776
28824
9025
18016
15103
18931
28602
8158
17472
1453
1990



16594
2573
20483
22565
23359
4684
14747
21853
24807
9107
9108
22028



24031
21991
26569
29011
5830
3328
15099
15130
21719
26251
13720
2076



22740
19986
24231
21220
18601
2781
26295
4537
1807
15313
11247
9328



2456
2455
2459
13267
13269
451
450
9730
9757
9762
21426
14657



11571
10874
15508
22276
22496
21391
21149
21996
21999
14338
14342
14140



14341
14363
15131
6669
14372
14890
14882
14886
14908
30442
13868
10627



14633
10628
10603
9996
18761
17083
26475
9727
16415
22604
16765
21893



21349
22764
341
3311
21992
8269
28346
13382
24297
24316
13140
2970



22223
763
16890
3910
16235
11545
28077
2792
23797
2309
7723
10108



12589
10780
13561
10432
24018
30465
23985
23865
12964
15841
26552
12280



10917


589:
21641
11914
23866
8645
13613
9312
23237
9134
23388
20043
2701
16767



28224
13756
18982
19274
23559
11811
2974
2235
28082
19162
8555
18862



24954
8256
20898
18136
10706
4529
15333
26671
4930
16619
29659
25935



24554
12329
15453
22322
9282
7014
23385
24862
14339
14577
13104
29724



6660


590:
1370
1803
10479
6604
11379
22320
2150
19084
8835
21367
3715
27142



28468
16052
29977
20304
15104
17160
22606
11506
10642
20938
19470
5997



2431
12142
10998
8140
2012
692


591:
20634
29046
27023
27018
27020
15445
17319
8384
8435
8439
4475
26372



26378
20484
17008
16211
767
2381
11826
27301
16213
17011
3572
27818



17652
27668
7815
11633
10323
7206
6849
28423
21614
28759
10404
11171



1722
14717
6437
5521
13143
26232
22628
28738
15781
15775
15777
23480



13052
16215
17016
22902
27289
27188
24044
24290


592:
21053
5637
13367
30097
30098
1714
4293
13481
14355
1206
1181
7897



14745
9179
2803
2806
19460
10635
28838
25830
16687
19459
23427
9319



15848
15846
12390
12391
10033
21274
8353
18944
2176
14748
9271
11074



12439
7936
20697
13928
20737
15591
1381
1363
5045
5043
23810
15873



18769
26971
26972
9219
3214
3212
2831
2833
16064
6039
24993
24991



27439
27438
29207
1634
19383
24416
24418
16903
11802
13429
2494
28555



28557
4328
3269
3272
14997
1597
27111
25193
9655
13046
13048
6270



7198
27215
1146
26515
26517
21921
15283
27402
27868
9639
16209
8679



15709
27332
20494
13930
5116
6353
7756
7601
8986
9515
1362
1365



685
24332
25918
25928
20497
12076
973
974
7589
1314
2849
7200



21813
9155
9156
10841
19587
19589
6171
8235
16570
3531
3526
8286



3377
5657
22868
22870
1551
1553
26201
26375
6394
14686
14687
26817



2903
10287
16363
11145
5246
5250
14737
23734
23730
21861
21859
26687



29894
30434
30431
2899
2902
16334
5302
5298
9888
4303
10265
22367



18580
14695
27846
17626
2109
6052
16127
30216
4367
6687
26267
24728



24730
14722
14720
1445
27700
27697
1322
18938
17173
17172
14492
24191



26309
26311
18431
18429
22637
22639
1746
841
19542
25007
23073
921



8727
22508
11259
2295
28478
17819
26866
29146
1507
7960
7961
11492



26369
26371
18436
5241
24911
10101
10102
7319
7323
10634
18983
29268



29265
26149
26150
21925
21927
17972
18001
20748
20747
23042
30325
19845



24599
4808
29229
14641
14643
6370
15708
15710
29633
29632
21113
21114



11209
11213
22426
22424
29203
29204
29502
29488
4378
26546
26549
4062



18589
26724
6332
23821
27135
22196
16034
11065
11709
23015
18600
2580



29261
27119
27964
27971
11480
11481
9572
17430
17428
9575
7945
14729



8825
26717
26720
9302
9326
18620
924
27200
20233
10699
27257
26880



26881
12995
17825
17823
24741
22069
19432
19359
13970
3496
1711
23383



21158
12866
30263
18787
26538
14868
4035
4388
10801
10632
25632
23963



28612
23253
27927
1178
29132
20213
1016
14461
24758
23386
21388
5313



5058
28289
4759
4761
4762
2437
12185
12187
18988
1713
28792
18380



15839
14194
18840
4330
4864
8121
9335
10310
10638
18392
21281
20942



20443
26148
20072
21300
12484
2275
25950
3671
29507
16628
10059
2250



1723
882
29614
22890
7292
3148
16143
24227
8787
10741
1326
5613



9289
9306
865
6419
4798
13777
13774
17747
17712
708
16833
3004



17056
6800
2653
20042
17162
26198
26200
27852
24966
24968
11426
30306



30308
5191
6541
20215
20217
1772
3268
4046
4053
4050
3207
3027



3022
4057
3074
3029
3173
3168
12543
10664
21634
17128
6401
12365



8519
13988
13986
30242
15429
9261
23736
7773
12871
20211
20212
2210



10272
16332
16353
5091
25709
22283
2518
559
3861
19815
28848
16627



1790
20457


593:
23100
8437
16789
9184
9170
14972
26126
2782
2808
8912
12593
5659



27751
17249
847
16077
17003
17564
17566
9675
16458
25308
11810
6462



19004
23303
25333
8046
1432
22200
23141
1516
3596
26032
1858
3259



28746
25829
23426
25373
17115
18882
24257
4938
22448
11228
22286
22389



18549
21796
28404
16829
23612
23617
23616
23645
23590
25680
25614
3975



18943
21410
15360
23410
8549
26330
4056
30346
24472
24445
15829
27029



27626
7896
28162
7743
11962
15101
26380
30303
26492
1890
15003
15005



17406
17853
5309
7867
3683
23220
12178
1817
12021
3159
5110
21308



29714
30354
28815
2223
12072
23205
2188
19401
16746
23172
5164
19927



20771
7518
23417
13391
12324
29494
4043
27813
29038
29780
28610
19153



7533
13762
4157
16421
4895
27867
25304
17568
17567
21063
16780
13258



11850
1334
24652
24325
18753
5836
16750
11824
20390
3170
13418
12249



20037
15769
5466
11682
25338
15918
9169
14001
3135
16677
18384
2213



9831
9001
2847
20465
14307
20286
10381
14305
30233
20568
24612
28359



13523
11725
13899
677
3809
24421
12358
12047
11941
29944
10425
20270



7425
12223
22959
25148
9300
8277
29986
21518
16545
25208
26145


594:
14081
8646
19510
3201
2969
21123
15598
14545
3412
24368
6958
8607



10353


595:
3687
13637
6857
13434
15892
15230
25954
18782
18783
18779
26319
3515



1014
21404
29758
1644
27147
15850
24399
9128
9154
16774
3773
27893



20486
12084
13600
29024
13597
29026
12676
4516
12392
10606
3631
16048



10151
20647
7798
14866
22177
6793
19027
4906
27511
7809
25075
26399



26044
1108
21212
3720
19569
14929
10537
11077
24782
21269
13233
23192



10539
24375
28382
7724
29220
7674
24131
28806
6795
28135
6830
26699



14196
28182
7465
6291
15138
29628
1123
15229
14863
15755
2030
14862



14269
17078
9228
4792
13137
8362
6931
8252
3767
14734
2995
13859



1957
3616
12222
2724
8691
15141
20150
4991
22712
22160
13892
14933



18671
18647
3672
8714
21442
25038
11854
6682
26742
6623
12833
1237



10466
10469
886
26275
17293
25839
20091
13855
18348
5981
13646
12691



10633
15139


596:
11135
17513
29853
2950
5458
29779
20336
13241
4799
29016
17076
11780



4104
12952
11732
12095
4999
16170
5457
3045
25718
6165
16051
8403



18273
6347
3040
24142
6392
3814
4226
20621
13649
27722
19640
9479



26129
15632
8041
25934
21263
12094
3514
30043
15134
24774
25723
29058



14294
10387
22923
13706
8226
14883
18231









Example 7
Selection of Transgenic Plants with Enhanced Agronomic Trait(s)

This example illustrates identification of plant cells of the invention by screening derived plants and seeds for enhanced trait. Transgenic seed and plants in corn, soybean, cotton or canola with recombinant DNA constructs identified in Table 2 are prepared by plant cells transformed with DNA that is stably integrated into the genome of the corn cell. Progeny transgenic plants and seed of the transformed plant cells are screened for enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil as compared to control plants


A. Selection for Enhanced Nitrogen Use Efficiency

Transgenic corn seeds provided by the present invention are planted in fields with three levels of nitrogen (N) fertilizer being applied, i.e. low level (0 N), medium level (80 lb/ac) and high level (180 lb/ac). A variety of physiological traits are monitored. Plants with enhanced NUE provide higher yield as compared to control plants.


B. Selection for Increased Yield

Effective selection of enhanced yielding transgenic plants uses hybrid progeny of the transgenic plants for corn, cotton, and canola, or inbred progeny of transgenic plants for soybeanplants plant such as corn, cotton, canola, or inbred plant such as soy, canola and cottoncotton over multiple locations with plants grown under optimal production management practices, and maximum pest control. A useful target for improved yield is a 5% to 10% increase in yield as compared to yield produced by plants grown from seed for a control plant. Selection methods may be applied in multiple and diverse geographic locations, for example up to 16 or more locations, over one or more planting seasons, for example at least two planting seasons, to statistically distinguish yield improvement from natural environmental effects.


In one field trial, transgenic corn plants comprising corn G1543 like 2 transgene as set forth in NUC SEQ ID NO: 74 under a tissue preferred promoter, such as green tissue promoter, have been shown to have an increased yield.


C. Selection for Enhanced Water Use Efficiency (WUE)

The selection process imposes a water withholding period to induce stressdrought followed by watering. For example, for corn, a useful selection process imposes 3 drought/re-water cycles on plants over a total period of 15 days after an initial stress free growth period of 11 days. Each cycle consists of 5 days, with no water being applied for the first four days and a water quenching on the 5th day of the cycle. The primary phenotypes analyzed by the selection method are the changes in plant growth rate as determined by height and biomass during a vegetative drought treatment.


D. Selection for Growth Under Cold Stress

(1) Cold germination assay—Trays of transgenic and control seeds are placed in a growth chamber at 9.7° C. for 24 days (no light). Seeds having higher germination rates as compared to the control are identified.


(2) Cold field efficacy trial—A cold field efficacy trial is used to identify gene constructs that confer enhanced cold vigor at germination and early seedling growth under early spring planting field conditions in conventional-till and simulated no-till environments. Seeds are planted into the ground around two weeks before local farmers begin to plant corn so that a significant cold stress is exerted onto the crop, named as cold treatment. Seeds also are planted under local optimal planting conditions such that the crop has little or no exposure to cold condition, named as normal treatment. At each location, seeds are planted under both cold and normal conditions with 3 repetitions per treatment. Two temperature monitors are set up at each location to monitor both air and soil temperature daily.


Seed emergence is defined as the point when the growing shoot breaks the soil surface. The number of emerged seedlings in each plot is counted everyday from the day the earliest plot begins to emerge until no significant changes in emergence occur. In addition, for each planting date, the latest date when emergence is 0 in all plots is also recorded. Seedling vigor is also rated at V3-V4 stage before the average of corn plant height reaches 10 inches, with 1=excellent early growth, 5=Average growth and 9=poor growth. Days to 50% emergence, maximum percent emergence and seedling vigor are used to determine plants with enhanced cold tolerance.


E. Screens for Transgenic Plant Seeds with Increased Protein and/or Oil Levels


This example sets forth a high-throughput selection for identifying plant seeds with improvement in seed composition using the Infratec 1200 series Grain Analyzer, which is a near-infrared transmittance spectrometer used to determine the composition of a bulk seed sample (Table 9). Near infrared analysis is a non-destructive, high-throughput method that can analyze multiple traits in a single sample scan. An NIR calibration for the analytes of interest is used to predict the values of an unknown sample. The NIR spectrum is obtained for the sample and compared to the calibration using a complex chemometric software package that provides predicted values as well as information on how well the sample fits in the calibration.


Infratec Model 1221, 1225, or 1227 with transport module by Foss North America is used with cuvette, item #1000-4033, Foss North America or for small samples with small cell cuvette, Foss standard cuvette modified by Leon Girard Co. Corn and soy check samples of varying composition maintained in check cell cuvettes are supplied by Leon Girard Co. NIT collection software is provided by Maximum Consulting Inc.Software. Calculations are performed automatically by the software. Seed samples are received in packets or containers with barcode labels from the customer. The seed is poured into the cuvettes and analyzed as received.










TABLE 9







Typical sample(s):
Whole grain corn and soybean seeds


Analytical time to run method:
Less than 0.75 min per sample


Total elapsed time per run:
1.5 minute per sample


Typical and minimum sample
Corn typical: 50 cc; minimum 30 cc


size:
Soybean typical: 50 cc; minimum 5 cc


Typical analytical range:
Determined in part by the specific



calibration.



Corn - moisture 5-15%, oil 5-20%,



protein 5-30%, starch 50-75%, and



density 1.0-1.3%.



Soybean - moisture 5-15%, oil 15-25%,



and protein 35-50%.









Example 8
Consensus Sequence

This example illustrates the identification of consensus amino acid sequence for the proteins and homologs encoded by DNA that is used to prepare the transgenic seed and plants of this invention having enhanced agronomic traits.


ClustalW program was selected for multiple sequence alignments of the amino acid sequence of SEQ ID NO: 301 and its 20 homologs. Three major factors affecting the sequence alignments dramatically are (1) protein weight matrices; (2) gap open penalty; (3) gap extension penalty. Protein weight matrices available for ClustalW program include Blosum, Pam and Gonnet series. Those parameters with gap open penalty and gap extension penalty were extensively tested. On the basis of the test results, Blosum weight matrix, gap open penalty of 10 and gap extension penalty of 1 were chosen for multiple sequence alignment. FIG. 1 shows the sequences of SEQ ID NO: 301, its homologs and the consensus sequence (SEQ ID NO: 30526) at the end. The symbols for consensus sequence are (1) uppercase letters for 100% identity in all positions of multiple sequence alignment output; (2) lowercase letters for >=70% identity; symbol; (3) “X” indicated <70% identity; (4) dashes “−” meaning that gaps were in >=70% sequences.


The consensus amino acid sequence can be used to identify DNA corresponding to the full scope of this invention that is useful in providing transgenic plants, for example corn and soybean plants with enhanced agronomic traits, for example improved nitrogen use efficiency, improved yield, improved water use efficiency and/or improved growth under cold stress, due to the expression in the plants of DNA encoding a protein with amino acid sequence identical to the consensus amino acid sequence.


Example 9
Identification of Amino Acid Domain by Pfam Analysis

This example illustrates the identification of domain and domain module by Pfam analysis.


The amino acid sequence of the expressed proteins that are shown to be associated with an enhanced trait are analyzed for Pfam protein family against the current Pfam collection of multiple sequence alignments and hidden Markov models using the HMMER software in the appended computer listing. The Pfam protein domains and modules for the proteins of SEQ ID NO: 299 through 596 are shown in Tables 10, 11 and 12. The Hidden Markov model databases for the identified patent families are also in the appended computer listing allowing identification of other homologous proteins and their cognate encoding DNA to enable the full breadth of the invention for a person of ordinary skill in the art. Certain proteins are identified by a single Pfam domain and others by multiple Pfam domains. For instance, the protein with amino acids of SEQ ID NO: 299 is characterized by three Pfam domains, i.e. KNOX1, KNOX2 and ELK.









TABLE 10







Pfam annotation













PEP








SEQ


ID NO
Gene ID
Pfam domain name
Begin
Stop
Score
E-value
















299
PHE0007721_18998.pep
KNOX1
102
146
90.4
5.50E−24


299
PHE0007721_18998.pep
KNOX2
153
204
101.2
3.10E−27


299
PHE0007721_18998.pep
ELK
242
263
37
6.50E−08


300
PHE0007756_18009.pep
RPE65
105
591
864
7.30E−257


302
PHE0004988_15925.pep
F-box
4
52
23.7
0.00064


302
PHE0004988_15925.pep
LRR_1
157
179
9.7
4.4


302
PHE0004988_15925.pep
LRR_2
290
314
14.1
0.19


303
PHE0007140_21771.pep
MtN3_slv
9
98
98.2
2.50E−26


303
PHE0007140_21771.pep
MtN3_slv
134
221
73.6
6.20E−19


304
PHE0006823_16403.pep
Globin
10
149
102.6
1.10E−27


305
PHE0007440_22555.pep
Miro
14
128
88.4
2.20E−23


305
PHE0007440_22555.pep
Ras
14
175
332.3
8.40E−97


306
PHE0000206_22432.pep
Pkinase
79
337
342.2
8.80E−100


306
PHE0000206_22432.pep
efhand
384
412
25
0.00026


306
PHE0000206_22432.pep
efhand
456
484
24.9
0.00028


306
PHE0000206_22432.pep
efhand
490
518
35.1
2.40E−07


307
PHE0000598_16824.pep
HLH
385
434
54.6
3.30E−13


308
PHE0007590_17883.pep
S1
2
70
92.7
1.10E−24


309
PHE0007588_17881.pep
S1
2
86
73.5
6.50E−19


310
PHE0007592_17885.pep
S1
1
75
48
3.20E−11


311
PHE0007587_17880.pep
S1
3
75
106.6
7.10E−29


312
PHE0007624_17923.pep
S1
2
72
45.6
1.70E−10


312
PHE0007624_17923.pep
eIF-1a
5
70
134.9
2.20E−37


313
PHE0007591_17884.pep
S1
2
68
63.9
5.20E−16


314
PHE0007623_17922.pep
CN_hydrolase
11
184
166.3
7.50E−47


315
PHE0007583_17871.pep
MtN3_slv
9
98
102.2
1.50E−27


315
PHE0007583_17871.pep
MtN3_slv
132
218
112.8
1.00E−30


316
PHE0006618_16146.pep
S10_plectin
3
98
240.6
3.40E−69


317
PHE0006791_16374.pep
GUN4
84
227
308.7
1.00E−89


318
PHE0007620_17918.pep
peroxidase
89
321
192.4
1.10E−54


319
PHE0006659_16195.pep
MFS_1
10
391
111.5
2.50E−30


321
PHE0006274_15867.pep
E2F_TDP
130
195
135.8
1.10E−37


322
PHE0006637_16166.pep
PP2C
7
305
255.6
1.00E−73


323
PHE0006926_16815.pep
VPS28
18
209
281.8
1.40E−81


324
PHE0006821_16402.pep
PALP
49
358
−65.8
0.00015


325
PHE0007687_18057.pep
p450
35
462
124
4.20E−34


326
PHE0007642_17999.pep
PAS
186
300
15.6
0.014


326
PHE0007642_17999.pep
PAS_3
211
303
25.3
0.00022


326
PHE0007642_17999.pep
PAS
464
578
17.8
0.0089


326
PHE0007642_17999.pep
PAS_3
489
581
23.5
0.00052


326
PHE0007642_17999.pep
Pkinase
663
952
269.7
5.80E−78


327
PHE0007677_18052.pep
FAD_binding_3
5
372
−96.1
4.10E−05


328
PHE0007644_18001.pep
LRRNT_2
23
64
49.2
1.40E−11


328
PHE0007644_18001.pep
LRR_1
97
119
10.5
3.1


328
PHE0007644_18001.pep
LRR_1
121
143
14.4
0.42


328
PHE0007644_18001.pep
LRR_1
145
167
12.1
1.5


328
PHE0007644_18001.pep
LRR_1
169
192
13
1.1


328
PHE0007644_18001.pep
LRR_1
194
216
14.1
0.52


328
PHE0007644_18001.pep
Pkinase
414
716
57.1
5.60E−14


329
PHE0007640_17992.pep
CorA
66
448
438.4
9.30E−129


330
PHE0007678_18053.pep
4HBT
66
151
56.4
9.40E−14


331
PHE0007649_18006.pep
Ank
108
140
50.4
6.20E−12


331
PHE0007649_18006.pep
Pkinase
196
455
113
8.80E−31


331
PHE0007649_18006.pep
Pkinase_Tyr
196
455
94.9
2.40E−25


332
PHE0006726_16298.pep
WD40
45
83
30.2
7.30E−06


332
PHE0006726_16298.pep
WD40
135
173
38.6
2.10E−08


333
PHE0006218_8776.pep
Pyr_redox_2
85
370
163.7
4.70E−46


333
PHE0006218_8776.pep
Pyr_redox
231
325
93.9
4.80E−25


333
PHE0006218_8776.pep
Thioredoxin
423
528
25
1.50E−06


333
PHE0006218_8776.pep
Glutaredoxin
446
508
27.6
4.30E−05


335
PHE0002554_17876.pep
Chloroa_b-bind
63
261
263.3
5.00E−76


336
PHE0006807_16388.pep
CCT
352
390
76
1.20E−19


337
PHE0003359_8487.pep
p450
51
489
120.5
4.90E−33


338
PHE0006542_15765.pep
PMSR
92
245
271
2.30E−78


339
PHE0007622_17921.pep
Spermine_synth
34
279
249.7
6.10E−72


340
PHE0007630_17956.pep
2OG-FeII_Oxy
190
291
149.7
7.90E−42


341
PHE0007593_17886.pep
Spermine_synth
28
273
503.6
2.20E−148


342
PHE0006475_15588.pep
PsbQ
3
162
209.5
7.60E−60


343
PHE0007619_17915.pep
MBD
11
84
65.4
1.80E−16


344
PHE0006846_16447.pep
Sterol_desat
1
186
76.3
9.30E−20


345
PHE0007584_17874.pep
MBD
4
77
81.3
3.00E−21


346
PHE0003695_17913.pep
LRRNT_2
23
64
41.6
2.80E−09


346
PHE0003695_17913.pep
LRR_1
93
115
14.3
0.45


346
PHE0003695_17913.pep
LRR_1
117
139
18.3
0.028


346
PHE0003695_17913.pep
LRR_1
141
163
9.2
5.5


346
PHE0003695_17913.pep
LRR_1
165
186
12.7
1.2


346
PHE0003695_17913.pep
LRR_1
189
211
10.1
3.6


346
PHE0003695_17913.pep
LRR_1
213
235
9.8
4.3


346
PHE0003695_17913.pep
LRR_1
237
259
14.8
0.32


346
PHE0003695_17913.pep
LRR_1
260
281
10.6
2.9


346
PHE0003695_17913.pep
LRR_1
284
306
17.4
0.053


346
PHE0003695_17913.pep
LRR_1
308
330
13.8
0.65


346
PHE0003695_17913.pep
LRR_1
332
354
19.3
0.014


346
PHE0003695_17913.pep
LRR_1
356
378
9
5.8


346
PHE0003695_17913.pep
LRR_1
380
402
15.7
0.17


346
PHE0003695_17913.pep
LRR_1
404
426
11.8
1.8


346
PHE0003695_17913.pep
LRR_1
428
450
18.8
0.02


346
PHE0003695_17913.pep
LRR_1
452
474
13.2
0.93


346
PHE0003695_17913.pep
LRR_1
476
498
13.1
1


346
PHE0003695_17913.pep
LRR_1
500
521
10.4
3.3


346
PHE0003695_17913.pep
LRR_1
523
542
14.4
0.42


346
PHE0003695_17913.pep
Pkinase
648
917
111.8
2.00E−30


346
PHE0003695_17913.pep
Pkinase_Tyr
648
914
90.4
5.40E−24


347
PHE0003695_17879.pep
LRRNT_2
23
64
41.6
2.80E−09


347
PHE0003695_17879.pep
LRR_1
93
115
14.3
0.45


347
PHE0003695_17879.pep
LRR_1
117
139
18.3
0.028


347
PHE0003695_17879.pep
LRR_1
141
163
9.2
5.5


347
PHE0003695_17879.pep
LRR_1
165
186
12.7
1.2


347
PHE0003695_17879.pep
LRR_1
189
211
10.1
3.6


347
PHE0003695_17879.pep
LRR_1
213
235
9.8
4.3


347
PHE0003695_17879.pep
LRR_1
237
259
14.8
0.32


347
PHE0003695_17879.pep
LRR_1
260
281
10.6
2.9


347
PHE0003695_17879.pep
LRR_1
284
306
17.4
0.053


347
PHE0003695_17879.pep
LRR_1
308
330
13.8
0.65


347
PHE0003695_17879.pep
LRR_1
332
354
19.3
0.014


347
PHE0003695_17879.pep
LRR_1
356
378
9
5.8


347
PHE0003695_17879.pep
LRR_1
380
402
15.7
0.17


347
PHE0003695_17879.pep
LRR_1
404
426
11.8
1.8


347
PHE0003695_17879.pep
LRR_1
428
450
18.8
0.02


347
PHE0003695_17879.pep
LRR_1
452
474
13.2
0.93


347
PHE0003695_17879.pep
LRR_1
476
498
13.1
1


347
PHE0003695_17879.pep
LRR_1
500
521
10.4
3.3


347
PHE0003695_17879.pep
LRR_1
523
542
14.4
0.42


347
PHE0003695_17879.pep
Pkinase
648
917
111.8
2.00E−30


347
PHE0003695_17879.pep
Pkinase_Tyr
648
914
90.4
5.40E−24


349
PHE0007645_18002.pep
AP2
115
180
156.4
7.20E−44


350
PHE0006784_16366.pep
PALP
12
301
453.6
2.50E−133


351
PHE0006171_16491.pep
Glyoxalase
70
214
136.8
5.90E−38


353
PHE0006906_16796.pep
Proteasome
10
206
145.9
1.00E−40


354
PHE0006649_16180.pep
AP2
46
110
149.5
8.60E−42


355
PHE0006809_16390.pep
Prismane
1
548
886.8
9.80E−264


356
PHE0006918_16808.pep
SWIM
321
353
42.8
1.10E−09


357
PHE0007813_18219.pep
MtN3_slv
9
98
79.8
8.50E−21


357
PHE0007813_18219.pep
MtN3_slv
132
218
125.3
1.80E−34


359
PHE0006825_16405.pep
Cytochrom_C552
27
476
1333.6
0


360
PHE0006964_16864.pep
tRNA_anti
43
122
55.2
2.10E−13


360
PHE0006964_16864.pep
tRNA-synt_2
140
557
208.2
1.90E−59


361
PHE0008105_18407.pep
Histone
152
225
69.9
8.40E−18


361
PHE0008105_18407.pep
CBFD_NFYB_HMF
158
222
77.9
3.10E−20


362
PHE0006630_16159.pep
Ldh_1_N
90
239
202
1.30E−57


362
PHE0006630_16159.pep
Ldh_1_C
241
413
258.1
1.80E−74


363
PHE0006966_16868.pep
DNA_photolyase
12
183
298.7
1.10E−86


363
PHE0006966_16868.pep
FAD_binding_7
214
492
536.3
3.30E−158


365
PHE0006608_16123.pep
GSHPx
42
150
237.8
2.30E−68


366
PHE0010462_21459.pep
NUDIX
17
159
111.7
2.20E−30


366
PHE0010462_21459.pep
NUDIX
190
322
100.5
4.90E−27


367
PHE0009211_21774.pep
Auxin_inducible
1
106
149.5
8.80E−42


368
PHE0006256_8775.pep
RPE65
105
591
864.5
5.10E−257


369
PHE0006989_16918.pep
Pkinase
88
396
224.8
1.90E−64


370
PHE0007649_18166.pep
Ank
108
140
50.4
6.20E−12


370
PHE0007649_18166.pep
Pkinase
196
455
115.1
2.00E−31


370
PHE0007649_18166.pep
Pkinase_Tyr
196
455
98.9
1.50E−26


371
PHE0006708_16264.pep
DEAD
57
223
202.7
8.40E−58


371
PHE0006708_16264.pep
Helicase_C
291
367
127.8
3.10E−35


372
PHE0001117_19094.pep
Homeobox
79
133
68.2
2.70E−17


372
PHE0001117_19094.pep
HALZ
134
178
66.7
7.20E−17


373
PHE0006266_8814.pep
Fe_bilin_red
16
244
209.5
7.50E−60


374
PHE0006795_16378.pep
Na_H_antiport_1
4
380
875.9
1.90E−260


375
PHE0006956_16853.pep
Molybdop_Fe4S4
2
56
92
1.90E−24


375
PHE0006956_16853.pep
Molybdopterin
59
482
377.8
1.60E−110


375
PHE0006956_16853.pep
Molydop_binding
578
689
159.8
7.10E−45


375
PHE0006956_16853.pep
Fer2_BFD
825
877
62
2.00E−15


377
PHE0006824_16404.pep
Globin
14
154
93.4
7.00E−25


378
PHE0006812_16393.pep
Bac_globin
3
118
188.4
1.70E−53


379
PHE0006815_16396.pep
Bac_globin
26
146
191.4
2.10E−54


380
PHE0006806_16387.pep
Bac_globin
26
146
196.8
5.10E−56


381
PHE0006624_16153.pep
NAD_binding_2
3
178
282.5
7.90E−82


381
PHE0006624_16153.pep
6PGD
182
473
713.8
1.20E−211


382
PHE0005006_15823.pep
Chloroa_b-bind
63
242
241.4
1.90E−69


384
PHE0006683_16229.pep
Response_reg
10
151
73.8
5.40E−19


385
PHE0006884_16703.pep
Glyco_transf_5
83
344
462.7
4.80E−136


385
PHE0006884_16703.pep
Glycos_transf_1
384
567
40.2
7.10E−09


386
PHE0006691_16237.pep
NAD_Gly3P_dh_N
6
165
285
1.40E−82


386
PHE0006691_16237.pep
ApbA
8
169
6.6
0.00011


386
PHE0006691_16237.pep
NAD_Gly3P_dh_C
183
327
289.1
8.20E−84


387
PHE0006790_16372.pep
Rieske
220
317
96.7
7.10E−26


387
PHE0006790_16372.pep
PaO
408
507
180.7
3.60E−51


388
PHE0009143_19932.pep
Histone_HNS
21
129
170.1
5.70E−48


389
PHE0008406_18830.pep
AA_permease
57
511
619.8
2.40E−183


390
PHE0008279_18708.pep
Mit_rib_S27
14
93
135.3
1.70E−37


391
PHE0009142_19931.pep
Histone_HNS
21
130
175.1
1.70E−49


393
PHE0007639_17991.pep
ELFV_dehydrog_N
84
214
298.9
9.20E−87


393
PHE0007639_17991.pep
ELFV_dehydrog
229
474
469.7
3.60E−138


394
PHE0007646_18170.pep
F-box
36
83
51.8
2.20E−12


394
PHE0007646_18170.pep
Kelch_1
131
185
25.1
0.00026


394
PHE0007646_18170.pep
Kelch_1
187
233
44.2
4.40E−10


394
PHE0007646_18170.pep
Kelch_2
187
233
26.1
0.00012


395
PHE0007765_18182.pep
GST_N
5
79
69.6
1.00E−17


395
PHE0007765_18182.pep
GST_C
101
204
28.8
1.90E−05


396
PHE0008167_18465.pep
Pkinase
8
262
210
5.60E−60


396
PHE0008167_18465.pep
Pkinase_Tyr
8
262
247.9
2.10E−71


398
PHE0008183_18479.pep
LRRNT_2
23
66
49.3
1.30E−11


398
PHE0008183_18479.pep
LRR_1
71
93
12.1
1.6


398
PHE0008183_18479.pep
LRR_1
95
117
10.3
3.3


398
PHE0008183_18479.pep
LRR_1
119
142
13
1


398
PHE0008183_18479.pep
LRR_1
144
166
19.5
0.012


398
PHE0008183_18479.pep
LRR_1
168
190
10.6
3


398
PHE0008183_18479.pep
LRR_1
192
214
8.8
6.4


398
PHE0008183_18479.pep
LRR_1
289
311
17.3
0.055


398
PHE0008183_18479.pep
LRR_1
313
335
10.6
2.9


398
PHE0008183_18479.pep
LRR_1
337
359
10.8
2.8


398
PHE0008183_18479.pep
LRR_1
361
384
12
1.6


398
PHE0008183_18479.pep
LRR_1
409
431
10.4
3.2


398
PHE0008183_18479.pep
LRR_1
457
479
11.9
1.7


398
PHE0008183_18479.pep
LRR_1
481
503
10.2
3.4


398
PHE0008183_18479.pep
LRR_1
505
527
10.4
3.3


398
PHE0008183_18479.pep
LRR_1
529
551
10.9
2.6


398
PHE0008183_18479.pep
LRR_1
553
575
9.3
5.2


398
PHE0008183_18479.pep
LRR_1
577
598
11
2.4


398
PHE0008183_18479.pep
Pkinase
695
966
133.8
4.80E−37


398
PHE0008183_18479.pep
Pkinase_Tyr
695
966
134.8
2.30E−37


399
PHE0007585_17875.pep
LEA_3
2
98
138.9
1.30E−38


400
PHE0006804_16385.pep
zf-CCCH
13
39
35.4
1.90E−07


400
PHE0006804_16385.pep
zf-CCCH
149
174
24.3
0.00045


401
PHE0006703_15751.pep
PFK
72
347
650.2
1.70E−192


402
PHE0007410_17653.pep
Acyltransferase
27
249
29.2
1.00E−05


403
PHE0006854_16456.pep
YABBY
40
278
249.1
9.30E−72


404
PHE0007721_21293.pep
KNOX1
102
146
90.4
5.50E−24


404
PHE0007721_21293.pep
KNOX2
153
204
101.2
3.10E−27


404
PHE0007721_21293.pep
ELK
242
263
37
6.50E−08


405
PHE0006563_15990.pep
GATase_2
2
161
103.9
4.70E−28


405
PHE0006563_15990.pep
Asn_synthase
209
450
328.7
9.80E−96


406
PHE0006619_16147.pep
AA_kinase
15
261
192.3
1.20E−54


407
PHE0006813_16394.pep
Bac_globin
7
124
123
8.50E−34


408
PHE0006688_16234.pep
Ribosomal_S2
13
229
448.6
8.00E−132


409
PHE0006635_16164.pep
MGS
28
121
99.4
1.10E−26


411
PHE0008416_18838.pep
PTR2
115
504
350.4
3.00E−102


413
PHE0008599_19166.pep
Isoamylase_N
24
115
93.4
6.60E−25


413
PHE0008599_19166.pep
Alpha-amylase
164
578
75.8
1.40E−19


414
PHE0008375_18732.pep
Pribosyltran
93
237
141.8
1.80E−39


416
PHE0008394_18763.pep
Gln-synt_N
68
148
147.3
4.20E−41


416
PHE0008394_18763.pep
Gln-synt_C
154
406
287.7
2.20E−83


417
PHE0008395_18764.pep
Gln-synt_N
68
148
147.3
4.20E−41


417
PHE0008395_18764.pep
Gln-synt_C
154
406
285
1.50E−82


418
PHE0008444_18846.pep
Gln-synt_N
17
97
150.4
4.70E−42


418
PHE0008444_18846.pep
Gln-synt_C
103
355
283.9
3.00E−82


419
PHE0006819_16400.pep
Lactamase_B
47
236
77.1
5.60E−20


419
PHE0006819_16400.pep
Flavodoxin_1
267
397
8.1
0.0041


419
PHE0006819_16400.pep
Flavin_Reduct
424
573
254.7
1.90E−73


420
PHE0007417_17663.pep
EIN3
60
467
777.5
7.80E−231


421
PHE0008443_18845.pep
GATase_2
2
161
98.7
1.70E−26


421
PHE0008443_18845.pep
Asn_synthase
209
450
331
2.00E−96


422
PHE0006818_16399.pep
Lactamase_B
34
227
105.3
1.80E−28


422
PHE0006818_16399.pep
Flavodoxin_1
256
389
86.4
8.80E−23


422
PHE0006818_16399.pep
Rubredoxin
423
471
89.8
8.10E−24


423
PHE0006579_16031.pep
Thi4
63
306
552.2
5.40E−163


423
PHE0006579_16031.pep
DAO
91
319
−9
1.90E−05


424
PHE0006808_16389.pep
CCT
354
392
75.3
1.90E−19


425
PHE0008160_18460.pep
TPP_enzyme_N
45
221
286.5
5.20E−83


425
PHE0008160_18460.pep
TPP_enzyme_M
243
376
82.1
1.70E−21


425
PHE0008160_18460.pep
TPP_enzyme_C
431
578
34.8
2.80E−09


426
PHE0007571_17834.pep
GSH_synth_ATP
29
499
836.5
1.30E−248


426
PHE0007571_17834.pep
GSH_synthase
229
331
200
5.50E−57


427
PHE0007586_17877.pep
Chloroa_b-bind
64
261
255.3
1.20E−73


429
PHE0009729_21717.pep
Homeobox
67
123
68.9
1.60E−17


429
PHE0009729_21717.pep
HALZ
124
168
53.4
7.60E−13


430
PHE0006424_15520.pep
PsbW_2
1
133
382.1
8.60E−112


431
PHE0006392_15480.pep
PsbP
106
322
252.9
6.50E−73


432
PHE0006589_16093.pep
Thioredoxin
64
167
130.3
5.20E−36


433
PHE0009258_21487.pep
Na_H_Exchanger
22
441
181.7
1.70E−51


434
PHE0006680_16226.pep
2OG-FeII_Oxy
187
287
158
2.40E−44


435
PHE0006481_16072.pep
adh_short
33
181
28.8
7.00E−08


436
PHE0006269_8820.pep
TPR_1
74
107
21.3
0.0035


436
PHE0006269_8820.pep
TPR_2
74
107
24.7
0.00034


436
PHE0006269_8820.pep
TPR_1
108
141
37.4
4.80E−08


436
PHE0006269_8820.pep
TPR_2
108
141
35.3
2.10E−07


436
PHE0006269_8820.pep
TPR_1
142
175
15
0.086


436
PHE0006269_8820.pep
TPR_2
142
175
23.8
0.00063


436
PHE0006269_8820.pep
TPR_1
179
212
7.9
0.61


436
PHE0006269_8820.pep
TPR_4
247
272
23.6
0.00069


436
PHE0006269_8820.pep
TPR_1
281
314
16.2
0.063


436
PHE0006269_8820.pep
TPR_2
281
314
26
0.00014


436
PHE0006269_8820.pep
TPR_1
349
382
13.6
0.13


437
PHE0006783_16365.pep
Aa_trans
32
469
574.9
7.90E−170


438
PHE0003151_18392.pep
AP2
318
391
149.5
8.70E−42


438
PHE0003151_18392.pep
AP2
420
485
125.8
1.20E−34


439
PHE0008272_18723.pep
HSF_DNA-bind
18
188
213.9
3.50E−61


440
PHE0008277_18706.pep
SNF5
172
400
471.8
8.60E−139


441
PHE0008109_18411.pep
Sad1_UNC
203
330
195.6
1.10E−55


442
PHE0008280_18826.pep
Alpha-amylase
14
452
198.9
1.20E−56


443
PHE0002424_15825.pep
adh_short
43
215
24.9
1.30E−07


443
PHE0002424_15825.pep
KR
43
200
−69.1
0.0024


444
PHE0006914_16804.pep
AAA
204
391
298.6
1.10E−86


445
PHE0006642_16172.pep
p450
42
496
213.5
4.80E−61


447
PHE0009926_21079.pep
DSPc
24
152
137.5
3.60E−38


448
PHE0006206_19159.pep
Sina
106
305
426.4
4.00E−125


449
PHE0008274_18705.pep
BURP
56
280
380
3.60E−111


450
PHE0009510_20526.pep
Spermine_synth
38
283
500.4
2.10E−147


451
PHE0009937_21097.pep
Spermine_synth
38
283
500.4
2.10E−147


452
PHE0009927_21080.pep
DSPc
24
162
146.6
6.60E−41


454
PHE0006445_15956.pep
Allene_ox_cyc
74
253
401.6
1.20E−117


455
PHE0006987_16893.pep
efhand
86
114
25
0.00026


455
PHE0006987_16893.pep
efhand
167
195
27.6
4.30E−05


456
PHE0004230_15865.pep
E2F_TDP
146
211
133.8
4.60E−37


457
PHE0006814_16395.pep
Bac_globin
26
144
206
8.80E−59


458
PHE0002773_15881.pep
NTP_transferase
88
366
415.4
8.30E−122


459
PHE0006060_15842.pep
ubiquitin
5
73
138
2.50E−38


459
PHE0006060_15842.pep
ubiquitin
81
149
138
2.50E−38


459
PHE0006060_15842.pep
ubiquitin
157
225
138
2.50E−38


459
PHE0006060_15842.pep
ubiquitin
233
301
138
2.50E−38


460
PHE0006850_16451.pep
YABBY
6
166
342.9
5.20E−100


461
PHE0008043_18199.pep
Homeobox
25
85
64.6
3.20E−16


461
PHE0008043_18199.pep
START
164
374
198.3
1.80E−56


461
PHE0008043_18199.pep
MEKHLA
693
840
296.7
4.40E−86


462
PHE0000125_18852.pep
Response_reg
28
153
25.4
3.80E−05


462
PHE0000125_18852.pep
CCT
457
495
70.6
5.10E−18


463
PHE0008269_18720.pep
Pkinase
23
279
234
3.30E−67


464
PHE0008264_18712.pep
zf-UBR
40
109
73.4
7.10E−19


465
PHE0002782_20059.pep
PGI
2
457
172.8
8.80E−49


466
PHE0009438_20404.pep
GATA
191
226
72.7
1.10E−18


467
PHE0009429_20392.pep
ParBc
70
151
47.5
4.50E−11


468
PHE0009440_20406.pep
ParBc
11
126
55.3
2.00E−13


469
PHE0008543_18945.pep
TP_methylase
222
435
272.6
7.70E−79


470
PHE0006960_16857.pep
Oxidored_molyb
116
301
289.2
7.70E−84


470
PHE0006960_16857.pep
Mo-co_dimer
326
459
300.9
2.30E−87


470
PHE0006960_16857.pep
Cyt-b5
515
588
93.3
7.20E−25


470
PHE0006960_16857.pep
FAD_binding_6
633
740
212.6
9.20E−61


470
PHE0006960_16857.pep
NAD_binding_1
759
872
169.6
8.00E−48


471
PHE0009427_20389.pep
IGPD
103
247
372.6
6.20E−109


472
PHE0006437_15906.pep
Pkinase
39
307
15.8
1.70E−09


473
PHE0007409_17652.pep
DAGAT
64
340
278.2
1.60E−80


474
PHE0009426_20387.pep
PHP
2
281
104.4
3.30E−28


475
PHE0009430_20393.pep
ParBc
44
128
45.7
1.60E−10


476
PHE0007003_16906.pep
PTPA
96
396
524
1.60E−154


477
PHE0008378_18741.pep
bZIP_1
223
280
76.5
8.20E−20


477
PHE0008378_18741.pep
bZIP_2
224
277
47.5
4.40E−11


478
PHE0008273_18725.pep
zf-CCCH
80
106
39.3
1.30E−08


479
PHE0008547_18953.pep
Fer2
20
95
95
2.20E−25


480
PHE0006922_16812.pep
Hexokinase_1
44
251
260.7
2.90E−75


480
PHE0006922_16812.pep
Hexokinase_2
258
501
261.2
2.10E−75


481
PHE0006920_16810.pep
AAA
267
458
270
4.60E−78


481
PHE0006920_16810.pep
Vps4_C
464
514
4.5
0.00031


482
PHE0008396_18765.pep
Gln-synt_N
68
148
147.3
4.20E−41


482
PHE0008396_18765.pep
Gln-synt_C
154
406
285.4
1.10E−82


483
PHE0006957_16854.pep
Gln-synt_N
68
148
147.3
4.20E−41


483
PHE0006957_16854.pep
Gln-synt_C
154
406
285.4
1.10E−82


484
PHE0006404_15936.pep
RRM_1
8
77
81.4
2.80E−21


484
PHE0006404_15936.pep
RRM_1
112
182
81.8
2.10E−21


485
PHE0006820_16401.pep
Lactamase_B
47
236
82.8
1.10E−21


485
PHE0006820_16401.pep
Flavin_Reduct
423
572
222.8
7.90E−64


486
PHE0006690_16236.pep
MFS_1
57
421
44.3
4.30E−10


487
PHE0006395_15932.pep
LRR_1
185
206
11.9
1.7


487
PHE0006395_15932.pep
LRR_1
208
229
17.1
0.065


487
PHE0006395_15932.pep
LRR_1
231
252
13.5
0.77


487
PHE0006395_15932.pep
LRR_1
254
272
12.6
1.2


487
PHE0006395_15932.pep
LRR_1
278
299
8.4
7.8


487
PHE0006395_15932.pep
LRR_1
349
370
20.8
0.0049


487
PHE0006395_15932.pep
LRR_1
372
394
7.8
9.8


488
PHE0006759_16384.pep
SHMT
55
453
1032.6
0


489
PHE0006576_16028.pep
Myb_DNA-binding
11
57
59.3
1.30E−14


489
PHE0006576_16028.pep
Myb_DNA-binding
63
108
48.9
1.70E−11


490
PHE0006979_16885.pep
Tryp_alpha_amyl
33
111
55.3
2.00E−13


491
PHE0009786_21754.pep
HLH
33
80
30
8.20E−06


492
PHE0006572_16007.pep
Pkinase
11
269
317
3.30E−92


494
PHE0006588_16092.pep
Thioredoxin
56
161
128.8
1.50E−35


495
PHE0006985_16891.pep
DUF1716
13
118
159.3
1.00E−44


496
PHE0006875_16688.pep
PSI_PSAK
44
138
191.6
1.90E−54


497
PHE0006626_16155.pep
Glyco_hydro_17
30
335
587.5
1.20E−173


498
PHE0006689_16235.pep
Aa_trans
165
548
224.1
3.20E−64


499
PHE0006799_16382.pep
B12-binding
10
135
104.3
3.70E−28


499
PHE0006799_16382.pep
Radical_SAM
202
368
91.2
3.10E−24


500
PHE0006959_16856.pep
Oxidored_molyb
143
328
286.7
4.40E−83


500
PHE0006959_16856.pep
Mo-co_dimer
353
486
297.6
2.40E−86


500
PHE0006959_16856.pep
Cyt-b5
543
616
90.4
5.60E−24


500
PHE0006959_16856.pep
FAD_binding_6
660
767
231.6
1.70E−66


500
PHE0006959_16856.pep
NAD_binding_1
786
899
186.4
7.00E−53


501
PHE0011615_23861.pep
Pkinase
267
523
297.5
2.50E−86


501
PHE0011615_23861.pep
Pkinase_Tyr
267
523
127.5
3.70E−35


502
PHE0006925_16814.pep
Tim17
4
133
198.1
2.10E−56


503
PHE0006713_16274.pep
APC8
1
161
401.5
1.20E−117


503
PHE0006713_16274.pep
TPR_1
339
372
34.7
3.20E−07


503
PHE0006713_16274.pep
TPR_2
339
372
23.7
0.00066


503
PHE0006713_16274.pep
TPR_2
373
406
23.7
0.00068


503
PHE0006713_16274.pep
TPR_1
373
406
34.1
4.80E−07


503
PHE0006713_16274.pep
TPR_2
407
440
22.1
0.002


503
PHE0006713_16274.pep
TPR_1
407
440
24.3
0.00043


504
PHE0008447_18848.pep
Lir1
1
126
178.6
1.60E−50


505
PHE0006674_16221.pep
PTR2
96
505
222.7
8.20E−64


506
PHE0010613_22398.pep
DAGK_cat
43
184
106.4
8.50E−29


507
PHE0002720_22558.pep
DnaJ
90
151
137.6
3.40E−38


507
PHE0002720_22558.pep
DnaJ_CXXCXGXG
218
301
100.5
5.20E−27


507
PHE0002720_22558.pep
DnaJ_C
314
436
210.6
3.60E−60


508
PHE0011760_24005.pep
Pkinase
400
656
297.5
2.50E−86


508
PHE0011760_24005.pep
Pkinase_Tyr
400
656
127.5
3.70E−35


509
PHE0010612_22397.pep
G-patch
15
59
62
1.90E−15


511
PHE0007411_17654.pep
Acyltransferase
136
283
86.4
8.50E−23


512
PHE0010617_22402.pep
Isochorismatase
27
210
−41
0.0027


513
PHE0010610_22395.pep
zf-C3HC4
103
144
36.3
1.10E−07


514
PHE0006883_16702.pep
Glyco_transf_5
78
339
461.4
1.10E−135


514
PHE0006883_16702.pep
Glycos_transf_1
379
562
40.5
5.80E−09


515
PHE0010636_22408.pep
UAA
96
387
−125.2
0.00037


515
PHE0010636_22408.pep
DUF6
105
230
32.1
2.00E−06


515
PHE0010636_22408.pep
TPT
239
384
188.8
1.30E−53


516
PHE0011082_23038.pep
adh_short
19
187
111.5
2.50E−30


516
PHE0011082_23038.pep
KR
19
202
−67.5
0.0019


517
PHE0009475_20462.pep
2OG-FeII_Oxy
223
322
166.9
5.20E−47


518
PHE0007415_17661.pep
EIN3
60
467
781.6
4.80E−232


519
PHE0006614_16129.pep
Cellulase
19
358
−25.9
1.00E−05


520
PHE0010611_22396.pep
FMO-like
25
424
−364.7
5.40E−07


521
PHE0011719_23946.pep
Acyltransferase
69
222
30
8.30E−06


522
PHE0006861_16463.pep
NPH3
166
409
387.6
1.80E−113


523
PHE0006568_16005.pep
DUF26
80
134
56.9
6.70E−14


523
PHE0006568_16005.pep
DUF26
199
253
62.1
1.80E−15


523
PHE0006568_16005.pep
Pkinase_Tyr
346
615
114
4.20E−31


523
PHE0006568_16005.pep
Pkinase
346
615
163.2
6.70E−46


524
PHE0010652_22429.pep
FtsJ
21
211
282.4
8.80E−82


525
PHE0008158_18448.pep
PLATZ
22
144
232.3
1.00E−66


526
PHE0003316_20755.pep
Glyco_hydro_2_N
9
180
238.7
1.30E−68


526
PHE0003316_20755.pep
Glyco_hydro_2
182
272
129.9
7.00E−36


526
PHE0003316_20755.pep
Glyco_hydro_2_C
274
593
612.1
5.00E−181


527
PHE0009478_20470.pep
2OG-Fell_Oxy
229
328
171.2
2.50E−48


528
PHE0010395_21760.pep
DUF716
109
266
180.5
4.10E−51


529
PHE0010391_21753.pep
DUF640
28
160
296.6
4.70E−86


530
PHE0010396_21761.pep
DUF716
109
265
175.2
1.60E−49


531
PHE0009511_22422.pep
SAM_decarbox
5
337
710.3
1.30E−210


532
PHE0010614_22399.pep
SOUL
28
211
330.7
2.50E−96


534
PHE0011454_23667.pep
Histone
56
126
103.3
7.20E−28


535
PHE0011443_24001.pep
Histone
61
131
103.3
7.20E−28


536
PHE0011452_23665.pep
Histone
58
132
142.7
9.80E−40


537
PHE0010394_21758.pep
SAP18
29
150
260.5
3.30E−75


540
PHE0010100_21467.pep
Pyr_redox_2
5
285
134.4
3.10E−37


540
PHE0010100_21467.pep
Pyr_redox
147
240
91
3.50E−24


540
PHE0010100_21467.pep
Fer2_BFD
420
472
81.5
2.60E−21


540
PHE0010100_21467.pep
NIR_SIR_ferr
554
621
89.9
7.80E−24


540
PHE0010100_21467.pep
NIR_SIR
629
775
176.5
6.70E−50


541
PHE0011503_23734.pep
GAF
158
307
91.9
1.90E−24


541
PHE0011503_23734.pep
HisKA
343
408
87.5
4.10E−23


541
PHE0011503_23734.pep
HATPase_c
455
586
123.9
4.50E−34


542
PHE0010099_21319.pep
Molybdop_Fe4S4
1
55
91.2
3.30E−24


542
PHE0010099_21319.pep
Molybdopterin
58
475
479.9
3.00E−141


542
PHE0010099_21319.pep
Molydop_binding
570
681
140
6.60E−39


542
PHE0010099_21319.pep
Fer2_BFD
811
863
74.8
2.70E−19


543
PHE0011269_23400.pep
Homeobox
21
78
70.4
5.80E−18


543
PHE0011269_23400.pep
START
215
437
167.3
3.90E−47


546
PHE0011613_23858.pep
AP2
62
136
129.6
8.70E−36


546
PHE0011613_23858.pep
AP2
165
230
109.9
7.50E−30


547
PHE0001582_22064.pep
GAF
195
344
57.2
5.30E−14


547
PHE0001582_22064.pep
HisKA
380
445
22.2
0.00068


547
PHE0001582_22064.pep
Response_reg
645
761
69.4
1.20E−17


548
PHE0007444_22314.pep
Spermine_synth
90
349
414.5
1.50E−121


549
PHE0010543_22298.pep
PBP
19
165
216.7
5.30E−62


550
PHE0010543_22323.pep
PBP
19
157
186
9.30E−53


551
PHE0011081_23033.pep
Sugar_tr
33
465
−7.2
1.20E−06


552
PHE0011075_23026.pep
P-II
73
175
181.5
2.10E−51


553
PHE0010100_21462.pep
Pyr_redox_2
5
285
134.4
3.10E−37


553
PHE0010100_21462.pep
Pyr_redox
147
240
91
3.50E−24


553
PHE0010100_21462.pep
Fer2_BFD
420
472
81.5
2.60E−21


553
PHE0010100_21462.pep
NIR_SIR_ferr
554
621
89.9
7.80E−24


553
PHE0010100_21462.pep
NIR_SIR
629
775
176.5
6.70E−50


554
PHE0009640_21775.pep
Auxin_inducible
1
102
151.5
2.10E−42


555
PHE0010092_21307.pep
S1
13
88
51.4
3.00E−12


555
PHE0010092_21307.pep
E1F_2_alpha
125
256
217.3
3.50E−62


556
PHE0004611_24123.pep
PTR2
13
406
283.1
5.40E−82


557
PHE0010093_21308.pep
S1
13
88
50
8.10E−12


557
PHE0010093_21308.pep
EIF_2_alpha
125
256
217.3
3.50E−62


558
PHE0008605_23084.pep
Histone
58
128
105.5
1.60E−28


559
PHE0009939_21100.pep
GDC-P
81
508
1024.4
0


560
PHE0009941_21105.pep
mTERF
2
262
117.5
3.70E−32


561
PHE0009951_21135.pep
Ribosomal_L10e
1
176
487.9
1.20E−143


562
PHE0009943_21127.pep
Ribosomal_L10e
1
176
460.5
2.20E−135


563
PHE0009948_21132.pep
Ribosomal_L10e
1
176
476.4
3.40E−140


564
PHE0011084_23040.pep
Peptidase_S10
54
482
561
1.20E−165


565
PHE0008233_23042.pep
Phi_1
40
314
616.9
1.80E−182


566
PHE0010854_22730.pep
RGS
294
412
23.2
2.80E−06


567
PHE0003797_23051.pep
zf-Dof
34
96
127
5.20E−35


568
PHE0010194_21769.pep
Glyco_hydro_9
52
509
1007.2
5.70E−300


570
PHE0011665_23899.pep
Chloroa_b-bind
63
260
255.3
1.20E−73


571
PHE0012178_24443.pep
Whirly
53
191
263.2
5.30E−76


573
PHE0011064_22994.pep
TPR_1
73
106
33.7
6.50E−07


573
PHE0011064_22994.pep
TPR_2
73
106
33.4
7.80E−07


577
PHE0011446_23659.pep
Histone
58
132
142.9
8.50E−40


578
PHE0001424_22077.pep
Agglutinin
55
193
46.9
6.30E−12


579
PHE0011445_23658.pep
C2
6
87
74.9
2.50E−19


580
PHE0010090_21297.pep
LRRNT_2
33
73
34.2
4.60E−07


580
PHE0010090_21297.pep
LRR_1
78
101
11.5
2


580
PHE0010090_21297.pep
LRR_1
103
127
11.2
2.3


580
PHE0010090_21297.pep
LRR_1
129
151
21.9
0.0024


580
PHE0010090_21297.pep
LRR_1
177
199
16
0.14


580
PHE0010090_21297.pep
LRR_1
226
248
10.3
3.3


580
PHE0010090_21297.pep
LRR_1
274
296
18.9
0.018


580
PHE0010090_21297.pep
LRR_1
298
320
12.1
1.6


580
PHE0010090_21297.pep
LRR_1
322
341
11.2
2.3


580
PHE0010090_21297.pep
LRR_1
395
417
9.8
4.1


580
PHE0010090_21297.pep
LRR_1
444
466
8.2
8.3


580
PHE0010090_21297.pep
LRR_1
468
490
12.8
1.1


580
PHE0010090_21297.pep
LRR_1
492
514
7.7
10


580
PHE0010090_21297.pep
LRR_1
516
538
15.7
0.17


580
PHE0010090_21297.pep
LRR_1
540
562
7.8
10


580
PHE0010090_21297.pep
LRR_1
564
585
8.5
7.4


580
PHE0010090_21297.pep
Pkinase
678
952
107.9
3.00E−29


580
PHE0010090_21297.pep
Pkinase_Tyr
678
952
110.4
5.10E−30


581
PHE0012180_24445.pep
B3
516
619
90.3
6.00E−24


582
PHE0011083_23039.pep
adh_short
44
200
46.7
7.60E−11


583
PHE0011085_23041.pep
Peptidase_S10
31
459
551.6
8.20E−163


584
PHE0012175_24440.pep
CXC
457
498
80.2
6.60E−21


584
PHE0012175_24440.pep
CXC
543
584
83.5
6.40E−22


585
PHE0012177_24442.pep
Whirly
91
229
325.2
1.10E−94


587
PHE0010194_21426.pep
Glyco_hydro_9
52
509
1013.8
5.70E−302


588
PHE0011666_23900.pep
Spermine_synth
46
291
503.1
3.20E−148


589
PHE0011448_24160.pep
Histone
25
90
35.4
1.90E−07


590
PHE0008324_18636.pep
DUF1005
256
460
524.8
9.30E−155


591
PHE0006971_16875.pep
Myb_DNA-binding
14
61
44.2
4.40E−10


591
PHE0006971_16875.pep
Myb_DNA-binding
67
112
51.3
3.20E−12


592
PHE0009939_21147.pep
GDC-P
81
508
1024.4
0


593
PHE0009953_21137.pep
Na_sulph_symp
110
578
831.2
5.40E−247


595
PHE0008557_18970.pep
NAD_binding_1
234
350
138.6
1.60E−38


596
PHE0006443_15955.pep
YDG SRA
360
519
403.5
3.00E−118


596
PHE0006443_15955.pep
Pre-SET
543
639
156.7
6.00E−44


596
PHE0006443_15955.pep
SET
641
771
181.7
1.80E−51
















TABLE 11







pfam module annotation










PEP SEQ





ID NO
Gene ID
Pfam domain module
Position





299
PHE0007721_18998.pep
KNOX1::KNOX2::ELK
102-146::153-204::242-263


300
PHE0007756_18009.pep
RPE65
105-591


302
PHE0004988_15925.pep
F-box::LRR_1::LRR_2
4-52::157-179::290-314


303
PHE0007140_21771.pep
MtN3_slv::MtN3_slv
9-98::134-221


304
PHE0006823_16403.pep
Globin
10-149


305
PHE0007440_22555.pep
Ras
14-175


306
PHE0000206_22432.pep
Pkinase::efhand::efhand::
79-337::384-412::456-




efhand
484::490-518


307
PHE0000598_16824.pep
HLH
385-434


308
PHE0007590_17883.pep
S1
2-70


309
PHE0007588_17881.pep
S1
2-86


310
PHE0007592_17885.pep
S1
1-75


311
PHE0007587_17880.pep
S1
3-75


312
PHE0007624_17923.pep
eIF-1a
5-70


313
PHE0007591_17884.pep
S1
2-68


314
PHE0007623_17922.pep
CN_hydrolase
11-184


315
PHE0007583_17871.pep
MtN3_slv::MtN3_slv
9-98::132-218


316
PHE0006618_16146.pep
S10_plectin
3-98


317
PHE0006791_16374.pep
GUN4
84-227


318
PHE0007620_17918.pep
peroxidase
89-321


319
PHE0006659_16195.pep
MFS_1
10-391


321
PHE0006274_15867.pep
E2F_TDP
130-195


322
PHE0006637_16166.pep
PP2C
7-305


323
PHE0006926_16815.pep
VPS28
18-209


324
PHE0006821_16402.pep
PALP
49-358


325
PHE0007687_18057.pep
p450
35-462


326
PHE0007642_17999.pep
PAS_3::PAS_3::Pkinase
211-303::489-581::663-952


327
PHE0007677_18052.pep
FAD_binding_3
5-372


328
PHE0007644_18001.pep
LRRNT_2::LRR_1::LRR_1::
23-64::97-119::121-143::145-




LRR_1::LRR_1::LRR_1::
167::169-192::194-216::414-716




Pkinase


329
PHE0007640_17992.pep
CorA
66-448


330
PHE0007678_18053.pep
4HBT
66-151


331
PHE0007649_18006.pep
Ank::Pkinase
108-140::196-455


332
PHE0006726_16298.pep
WD40::WD40
45-83::135-173


333
PHE0006218_8776.pep
Pyr_redox_2::Thioredoxin
85-370::423-528


335
PHE0002554_17876.pep
Chloroa_b-bind
63-261


336
PHE0006807_16388.pep
CCT
352-390


337
PHE0003359_8487.pep
p450
51-489


338
PHE0006542_15765.pep
PMSR
92-245


339
PHE0007622_17921.pep
Spermine_synth
34-279


340
PHE0007630_17956.pep
2OG-FeII_Oxy
190-291


341
PHE0007593_17886.pep
Spermine_synth
28-273


342
PHE0006475_15588.pep
PsbQ
3-162


343
PHE0007619_17915.pep
MBD
11-84


344
PHE0006846_16447.pep
Sterol_desat
1-186


345
PHE0007584_17874.pep
MBD
4-77


346
PHE0003695_17913.pep
LRRNT_2::LRR_1::LRR_1::
23-64::93-115::117-139::141-




LRR_1::LRR_1::LRR_1::
163::165-186::189-211::213-




LRR_1::LRR_1::LRR_1::
235::237-259::260-281::284-




LRR_1::LRR_1::LRR_1::
306::308-330::332-354::356-




LRR_1::LRR_1::LRR_1::
378::380-402::404-426::428-




LRR_1::LRR_1::LRR_1::
450::452-474::476-498::500-




LRR_1::LRR_1::Pkinase
521::523-542::648-917


347
PHE0003695_17879.pep
LRRNT_2::LRR_1::LRR_1::
23-64::93-115::117-139::141-




LRR_1::LRR_1::LRR_1::
163::165-186::189-211::213-




LRR_1::LRR_1::LRR_1::
235::237-259::260-281::284-




LRR_1::LRR_1::LRR_1::
306::308-330::332-354::356-




LRR_1::LRR_1::LRR_1::
378::380-402::404-426::428-




LRR_1::LRR_1::LRR_1::
450::452-474::476-498::500-




LRR_1::LRR_1::Pkinase
521::523-542::648-917


349
PHE0007645_18002.pep
AP2
115-180


350
PHE0006784_16366.pep
PALP
12-301


351
PHE0006171_16491.pep
Glyoxalase
70-214


353
PHE0006906_16796.pep
Proteasome
10-206


354
PHE0006649_16180.pep
AP2
46-110


355
PHE0006809_16390.pep
Prismane
1-548


356
PHE0006918_16808.pep
SWIM
321-353


357
PHE0007813_18219.pep
MtN3_slv::MtN3_slv
9-98::132-218


359
PHE0006825_16405.pep
Cytochrom_C552
27-476


360
PHE0006964_16864.pep
tRNA_anti::tRNA-synt_2
43-122::140-557


361
PHE0008105_18407.pep
CBFD_NFYB_HMF
158-222


362
PHE0006630_16159.pep
Ldh_1_N::Ldh_1_C
90-239::241-413


363
PHE0006966_16868.pep
DNA_photolyase::
12-183::214-492




FAD_binding_7


365
PHE0006608_16123.pep
GSHPx
42-150


366
PHE0010462_21459.pep
NUDIX::NUDIX
17-159::190-322


367
PHE0009211_21774.pep
Auxin_inducible
1-106


368
PHE0006256_8775.pep
RPE65
105-591


369
PHE0006989_16918.pep
Pkinase
88-396


370
PHE0007649_18166.pep
Ank::Pkinase
108-140::196-455


371
PHE0006708_16264.pep
DEAD::Helicase_C
57-223::291-367


372
PHE0001117_19094.pep
Homeobox::HALZ
79-133::134-178


373
PHE0006266_8814.pep
Fe_bilin_red
16-244


374
PHE0006795_16378.pep
Na_H_antiport_1
4-380


375
PHE0006956_16853.pep
Molybdop_Fe4S4::
2-56::59-482::578-689::825-




Molybdopterin::
877




Molydop_binding::




Fer2_BFD


377
PHE0006824_16404.pep
Globin
14-154


378
PHE0006812_16393.pep
Bac_globin
3-118


379
PHE0006815_16396.pep
Bac_globin
26-146


380
PHE0006806_16387.pep
Bac_globin
26-146


381
PHE0006624_16153.pep
NAD_binding_2::6PGD
3-178::182-473


382
PHE0005006_15823.pep
Chloroa_b-bind
63-242


384
PHE0006683_16229.pep
Response_reg
10-151


385
PHE0006884_16703.pep
Glyco_transf_5::
83-344::384-567




Glycos_transf_1


386
PHE0006691_16237.pep
NAD_Gly3P_dh_N::
6-165::183-327




NAD_Gly3P_dh_C


387
PHE0006790_16372.pep
Rieske::PaO
220-317::408-507


388
PHE0009143_19932.pep
Histone_HNS
21-129


389
PHE0008406_18830.pep
AA_permease
57-511


390
PHE0008279_18708.pep
Mit_rib_S27
14-93


391
PHE0009142_19931.pep
Histone_HNS
21-130


393
PHE0007639_17991.pep
ELFV_dehydrog_N::
84-214::229-474




ELFV_dehydrog


394
PHE0007646_18170.pep
F-box::Kelch_1::
36-83::131-185::187-233




Kelch_1


395
PHE0007765_18182.pep
GST_N::GST_C
5-79::101-204


396
PHE0008167_18465.pep
Pkinase_Tyr
8-262


398
PHE0008183_18479.pep
LRRNT_2::LRR_1::LRR_1::
23-66::71-93::95-117::119-




LRR_1::LRR_1::LRR_1::
142::144-166::168-190::192-




LRR_1::LRR_1::LRR_1::
214::289-311::313-335::337-




LRR_1::LRR_1::LRR_1::
359::361-384::409-431::457-




LRR_1::LRR_1::LRR_1::
479::481-503::505-527::529-




LRR_1::LRR_1::LRR_1::
551::553-575::577-598::695-




Pkinase_Tyr
966


399
PHE0007585_17875.pep
LEA_3
35827


400
PHE0006804_16385.pep
zf-CCCH::zf-CCCH
13-39::149-174


401
PHE0006703_15751.pep
PFK
72-347


402
PHE0007410_17653.pep
Acyltransferase
27-249


403
PHE0006854_16456.pep
YABBY
40-278


404
PHE0007721_21293.pep
KNOX1::KNOX2::ELK
102-146::153-204::242-263


405
PHE0006563_15990.pep
GATase_2::Asn_synthase
2-161::209-450


406
PHE0006619_16147.pep
AA_kinase
15-261


407
PHE0006813_16394.pep
Bac_globin
7-124


408
PHE0006688_16234.pep
Ribosomal_S2
13-229


409
PHE0006635_16164.pep
MGS
28-121


411
PHE0008416_18838.pep
PTR2
115-504


413
PHE0008599_19166.pep
Isoamylase_N::Alpha-
24-115::164-578




amylase


414
PHE0008375_18732.pep
Pribosyltran
93-237


416
PHE0008394_18763.pep
Gln-synt_N::Gln-synt_C
68-148::154-406


417
PHE0008395_18764.pep
Gln-synt_N::Gln-synt_C
68-148::154-406


418
PHE0008444_18846.pep
Gln-synt_N::Gln-synt_C
17-97::103-355


419
PHE0006819_16400.pep
Lactamase B::
47-236::267-397::424-573




Flavodoxin_1::




Flavin_Reduct


420
PHE0007417_17663.pep
EIN3
60-467


421
PHE0008443_18845.pep
GATase_2::Asn_synthase
2-161::209-450


422
PHE0006818_16399.pep
Lactamase B::Flavodoxin_1::
34-227::256-389::423-471




Rubredoxin


423
PHE0006579_16031.pep
Thi4
63-306


424
PHE0006808_16389.pep
CCT
354-392


425
PHE0008160_18460.pep
TPP_enzyme_N::
45-221::243-376::431-578




TPP_enzyme_M::




TPP_enzyme_C


426
PHE0007571_17834.pep
GSH_synth_ATP
29-499


427
PHE0007586_17877.pep
Chloroa_b-bind
64-261


429
PHE0009729_21717.pep
Homeobox::HALZ
67-123::124-168


430
PHE0006424_15520.pep
PsbW_2
1-133


431
PHE0006392_15480.pep
PsbP
106-322


432
PHE0006589_16093.pep
Thioredoxin
64-167


433
PHE0009258_21487.pep
Na_H_Exchanger
22-441


434
PHE0006680_16226.pep
2OG-FeII_Oxy
187-287


435
PHE0006481_16072.pep
adh_short
33-181


436
PHE0006269_8820.pep
TPR_2::TPR_1::TPR_2::
74-107::108-141::142-




TPR_1::TPR_4::TPR_2::
175::179-212::247-272::281-




TPR_1
314::349-382


437
PHE0006783_16365.pep
Aa_trans
32-469


438
PHE0003151_18392.pep
AP2::AP2
318-391::420-485


439
PHE0008272_18723.pep
HSF_DNA-bind
18-188


440
PHE0008277_18706.pep
SNF5
172-400


441
PHE0008109_18411.pep
Sad1_UNC
203-330


442
PHE0008280_18826.pep
Alpha-amylase
14-452


443
PHE0002424_15825.pep
adh_short
43-215


444
PHE0006914_16804.pep
AAA
204-391


445
PHE0006642_16172.pep
p450
42-496


447
PHE0009926_21079.pep
DSPc
24-152


448
PHE0006206_19159.pep
Sina
106-305


449
PHE0008274_18705.pep
BURP
56-280


450
PHE0009510_20526.pep
Spermine_synth
38-283


451
PHE0009937_21097.pep
Spermine_synth
38-283


452
PHE0009927_21080.pep
DSPc
24-162


454
PHE0006445_15956.pep
Allene_ox_cyc
74-253


455
PHE0006987_16893.pep
efhand::efhand
86-114::167-195


456
PHE0004230_15865.pep
E2F_TDP
146-211


457
PHE0006814_16395.pep
Bac_globin
26-144


458
PHE0002773_15881.pep
NTP_transferase
88-366


459
PHE0006060_15842.pep
ubiquitin::ubiquitin::
5-73::81-149::157-225::233-




ubiquitin::ubiquitin
301


460
PHE0006850_16451.pep
YABBY
6-166


461
PHE0008043_18199.pep
Homeobox::START::
25-85::164-374::693-840




MEKHLA


462
PHE0000125_18852.pep
Response_reg::CCT
28-153::457-495


463
PHE0008269_18720.pep
Pkinase
23-279


464
PHE0008264_18712.pep
zf-UBR
40-109


465
PHE0002782_20059.pep
PGI
2-457


466
PHE0009438_20404.pep
GATA
191-226


467
PHE0009429_20392.pep
ParBc
70-151


468
PHE0009440_20406.pep
ParBc
11-126


469
PHE0008543_18945.pep
TP_methylase
222-435


470
PHE0006960_16857.pep
Oxidored_molyb::
116-301::326-459::515-




Mo-co_dimer::Cyt-
588::633-740::759-872




b5::FAD_binding_6::




NAD_binding_1


471
PHE0009427_20389.pep
IGPD
103-247


472
PHE0006437_15906.pep
Pkinase
39-307


473
PHE0007409_17652.pep
DAGAT
64-340


474
PHE0009426_20387.pep
PHP
2-281


475
PHE0009430_20393.pep
ParBc
44-128


476
PHE0007003_16906.pep
PTPA
96-396


477
PHE0008378_18741.pep
bZIP 1
223-280


478
PHE0008273_18725.pep
zf-CCCH
80-106


479
PHE0008547_18953.pep
Fer2
20-95


480
PHE0006922_16812.pep
Hexokinase_1::
44-251::258-501




Hexokinase_2


481
PHE0006920_16810.pep
AAA::Vps4_C
267-458::464-514


482
PHE0008396_18765.pep
Gln-synt_N::Gln-synt_C
68-148::154-406


483
PHE0006957_16854.pep
Gln-synt_N::Gln-synt_C
68-148::154-406


484
PHE0006404_15936.pep
RRM_1::RRM_1
8-77::112-182


485
PHE0006820_16401.pep
Lactamase_B::Flavin_Reduct
47-236::423-572


486
PHE0006690_16236.pep
MFS_1
57-421


487
PHE0006395_15932.pep
LRR_1::LRR_1::LRR_1::
185-206::208-229::231-




LRR_1::LRR_1::LRR_1::
252::254-272::278-299::349-




LRR_1
370::372-394


488
PHE0006759_16384.pep
SHMT
55-453


489
PHE0006576_16028.pep
Myb_DNA-binding::
11-57::63-108




Myb_DNA-binding


490
PHE0006979_16885.pep
Tryp_alpha_amyl
33-111


491
PHE0009786_21754.pep
HLH
33-80


492
PHE0006572_16007.pep
Pkinase
11-269


494
PHE0006588_16092.pep
Thioredoxin
56-161


495
PHE0006985_16891.pep
DUF1716
13-118


496
PHE0006875_16688.pep
PS1_PSAK
44-138


497
PHE0006626_16155.pep
Glyco_hydro_17
30-335


498
PHE0006689_16235.pep
Aa_trans
165-548


499
PHE0006799_16382.pep
B12-binding::
10-135::202-368




Radical_SAM


500
PHE0006959_16856.pep
Oxidored_molyb::
143-328::353-486::543-




Mo-co_dimer::Cyt-b5::
616::660-767::786-899




FAD_binding_6::




NAD_binding_1


501
PHE0011615_23861.pep
Pkinase
267-523


502
PHE0006925_16814.pep
Tim17
4-133


503
PHE0006713_16274.pep
APC8::TPR_1::TPR_1::
l-161::339-372::373-




TPR_1
406::407-440


504
PHE0008447_18848.pep
Lir1
1-126


505
PHE0006674_16221.pep
PTR2
96-505


506
PHE0010613_22398.pep
DAGK_cat
43-184


507
PHE0002720_22558.pep
Dna_1::DnaJ_CXXCXGXG::
90-151::218-301::314-436




DnaJ_C


508
PHE0011760_24005.pep
Pkinase
400-656


509
PHE0010612_22397.pep
G-patch
15-59


511
PHE0007411_17654.pep
Acyltransferase
136-283


512
PHE0010617_22402.pep
Isochorismatase
27-210


513
PHE0010610_22395.pep
zf-C3HC4
103-144


514
PHE0006883_16702.pep
Glyco_transf_5::
78-339::379-562




Glycos_transf_1


515
PHE0010636_22408.pep
DUF6::TPT
105-230::239-384


516
PHE0011082_23038.pep
adh_short
19-187


517
PHE0009475_20462.pep
2OG-FeII_Oxy
223-322


518
PHE0007415_17661.pep
EIN3
60-467


519
PHE0006614_16129.pep
Cellulase
19-358


520
PHE0010611_22396.pep
FMO-like
25-424


521
PHE0011719_23946.pep
Acyltransferase
69-222


522
PHE0006861_16463.pep
NPH3
166-409


523
PHE0006568_16005.pep
DUF26::DUF26::Pkinase
80-134::199-253::346-615


524
PHE0010652_22429.pep
FtsJ
21-211


525
PHE0008158_18448.pep
PLATZ
22-144


526
PHE0003316_20755.pep
Glyco_hydro_2_N::
9-180::182-272::274-593




Glyco_hydro_2::




Glyco_hydro_2_C


527
PHE0009478_20470.pep
2OG-FeII_Oxy
229-328


528
PHE0010395_21760.pep
DUF716
109-266


529
PHE0010391_21753.pep
DUF640
28-160


530
PHE0010396_21761.pep
DUF716
109-265


531
PHE0009511_22422.pep
SAM_decarbox
5-337


532
PHE0010614_22399.pep
SOUL
28-211


534
PHE0011454_23667.pep
Histone
56-126


535
PHE0011443_24001.pep
Histone
61-131


536
PHE0011452_23665.pep
Histone
58-132


537
PHE0010394_21758.pep
SAP18
29-150


540
PHE0010100_21467.pep
Pyr_redox_2::Fer2_BFD::
5-285::420-472::554-




NIR_SIR_ferr::NIR_SIR
621::629-775


541
PHE0011503_23734.pep
GAF::HisKA::HATPase_c
158-307::343-408::455-586


542
PHE0010099_21319.pep
Molybdop_Fe4S4::
1-55::58-475::570-681::811-




Molybdopterin::
863




Molydop_binding::




Fer2_BFD


543
PHE0011269_23400.pep
Homeobox::START
21-78::215-437


546
PHE0011613_23858.pep
AP2::AP2
62-136::165-230


547
PHE0001582_22064.pep
GAF::HisKA::Response_reg
195-344::380-445::645-761


548
PHE0007444_22314.pep
Spermine_synth
90-349


549
PHE0010543_22298.pep
PBP
19-165


550
PHE0010543_22323.pep
PBP
19-157


551
PHE0011081_23033.pep
Sugar_tr
33-465


552
PHE0011075_23026.pep
P-II
73-175


553
PHE0010100_21462.pep
Pyr_redox_2::Fer2_BFD::
5-285::420-472::554-




NIR_SIR_ferr::NIR_SIR
621::629-775


554
PHE0009640_21775.pep
Auxin_inducible
1-102


555
PHE0010092_21307.pep
S1::EIF_2_alpha
13-88::125-256


556
PHE0004611_24123.pep
PTR2
13-406


557
PHE0010093_21308.pep
S1::EIF_2_alpha
13-88::125-256


558
PHE0008605_23084.pep
Histone
58-128


559
PHE0009939_21100.pep
GDC-P
81-508


560
PHE0009941_21105.pep
mTERF
2-262


561
PHE0009951_21135.pep
Ribosomal_L10e
1-176


562
PHE0009943_21127.pep
Ribosomal_L10e
1-176


563
PHE0009948_21132.pep
Ribosomal_L10e
1-176


564
PHE0011084_23040.pep
Peptidase_S10
54-482


565
PHE0008233_23042.pep
Phi_1
40-314


566
PHE0010854_22730.pep
RGS
294-412


567
PHE0003797_23051.pep
zf-Dof
34-96


568
PHE0010194_21769.pep
Glyco_hydro_9
52-509


570
PHE0011665_23899.pep
Chloroa_b-bind
63-260


571
PHE0012178_24443.pep
Whirly
53-191


573
PHE0011064_22994.pep
TPR_1
73-106


577
PHE0011446_23659.pep
Histone
58-132


578
PHE0001424_22077.pep
Agglutinin
55-193


579
PHE0011445_23658.pep
C2
6-87


580
PHE0010090_21297.pep
LRRNT_2::LRR_1::LRR_1::
33-73::78-101::103-127::129-




LRR_1::LRR_1::LRR_1::
151::177-199::226-248::274-




LRR_1::LRR_1::LRR_1::
296::298-320::322-341::395-




LRR_1::LRR_1::LRR_1::
417::444-466::468-490::492-




LRR_1::LRR_1::LRR_1::
514::516-538::540-562::564-




LRR_1::Pkinase_Tyr
585::678-952


581
PHE0012180_24445.pep
B3
516-619


582
PHE0011083_23039.pep
adh_short
44-200


583
PHE0011085_23041.pep
Peptidase_S10
31-459


584
PHE0012175_24440.pep
CXC::CXC
457-498::543-584


585
PHE0012177_24442.pep
Whirly
91-229


587
PHE0010194_21426.pep
Glyco_hydro_9
52-509


588
PHE0011666_23900.pep
Spermine_synth
46-291


589
PHE0011448_24160.pep
Histone
25-90


590
PHE0008324_18636.pep
DUF1005
256-460


591
PHE0006971_16875.pep
Myb_DNA_binding::
14-61::67-112




Myb_DNA-binding


592
PHE0009939_21147.pep
GDC-P
81-508


593
PHE0009953_21137.pep
Na_sulph_symp
110-578


595
PHE0008557_18970.pep
NAD_binding_1
234-350


596
PHE0006443_15955.pep
YDG_SRA::Pre-
360-519::543-639::641-771




SET::SET
















TABLE 12







Description of pfam domains











Accession
Gathering



Pfam domain name
number
cutoff
Domain description













2OG-FeII_Oxy
PF03171.10
11.5
2OG-Fe(II) oxygenase superfamily


4HBT
PF03061.12
20.6
Thioesterase superfamily


6PGD
PF00393.9
−232.3
6-phosphogluconate dehydrogenase,





C-terminal domain


AAA
PF00004.19
12.3
ATPase family associated with various





cellular activities (AAA)


AA_kinase
PF00696.18
−40
Amino acid kinase family


AA_permease
PF00324.11
−120.8
Amino acid permease


AP2
PF00847.10
0
AP2 domain


APC8
PF04049.4
−19.8
Anaphase promoting complex subunit 8/





Cdc23


Aa_trans
PF01490.8
−128.4
Transmembrane amino acid transporter





protein


Acyltransferase
PF01553.11
−0.4
Acyltransferase


Agglutinin
PF07468.2
25
Agglutinin


Allene_ox_cyc
PF06351.2
25
Allene oxide cyclase


Alpha-amylase
PF00128.14
−92.6
Alpha amylase, catalytic domain


Ank
PF00023.20
0
Ankyrin repeat


ApbA
PF02558.6
−16.2
Ketopantoate reductase PanE/ApbA


Asn_synthase
PF00733.11
−52.8
Asparagine synthase


Auxin_inducible
PF02519.5
−15
Auxin responsive protein


B12-binding
PF02310.9
19.6
B12 binding domain


B3
PF02362.12
26.5
B3 DNA binding domain


BURP
PF03181.6
−52
BURP domain


Bac_globin
PF01152.11
−12.7
Bacterial-like globin


C2
PF00168.20
3.7
C2 domain


CBFD_NFYB_HMF
PF00808.13
18.4
Histone-like transcription factor





(CBF/NF-Y) and archaeal histone


CCT
PF06203.4
25
CCT motif


CN_hydrolase
PF00795.12
−13.9
Carbon-nitrogen hydrolase


CXC
PF03638.5
25
Tesmin/TSO1-like CXC domain


Cellulase
PF00150.8
−49.3
Cellulase (glycosyl hydrolase family 5)


Chloroa_b-bind
PF00504.12
−31.9
Chlorophyll A-B binding protein


CorA
PF01544.9
−61.3
CorA-like Mg2+ transporter protein


Cyt-b5
PF00173.18
4
Cytochrome b5-like Heme/Steroid





binding domain


Cytochrom_C552
PF02335.6
−166
Cytochrome c552


DAGAT
PF03982.3
−151.7
Diacylglycerol acyltransferase


DAGK_cat
PF00781.14
−5.7
Diacylglycerol kinase catalytic domain





(presumed)


DAO
PF01266.14
−35
FAD dependent oxidoreductase


DEAD
PF00270.19
7.2
DEAD/DEAH box helicase


DNA_photolyase
PF00875.8
26.1
DNA photolyase


DSPc
PF00782.11
−21.8
Dual specificity phosphatase, catalytic





domain


DUF1005
PF06219.3
25
Protein of unknown function (DUF1005)


DUF1716
PF08216.2
25
Eukaryotic domain of unknown function





(DUF1716)


DUF26
PF01657.8
0
Domain of unknown function DUF26


DUF6
PF00892.11
20.8
Integral membrane protein DUF6


DUF640
PF04852.3
2.4
Protein of unknown function (DUF640)


DUF716
PF04819.3
−18.4
Family of unknown function (DUF716)


DnaJ
PF00226.21
−8
DnaJ domain


DnaJ_C
PF01556.9
−24
DnaJ C terminal region


DnaJ_CXXCXGXG
PF00684.9
1
DnaJ central domain (4 repeats)


E2F_TDP
PF02319.11
17
E2F/DP family winged-helix DNA-





binding domain


EIF_2_alpha
PF07541.2
−4.2
Eukaryotic translation initiation factor 2





alpha subunit


EEM3
PF04873.4
−137.6
Ethylene insensitive 3


ELFV_dehydrog
PF00208.11
−27
Glutamate/Leucine/Phenylalanine/Valine





dehydrogenase


ELFV_dehydrog_N
PF02812.8
31.8
Glu/Leu/Phe/Val dehydrogenase,





dimerisation domain


ELK
PF03789.4
25
ELK domain


F-box
PF00646.23
13.9
F-box domain


FAD_binding_3
PF01494.9
−136.6
FAD binding domain


FAD_binding_6
PF00970.14
−11.4
Oxidoreductase FAD-binding domain


FAD_binding_7
PF03441.4
25
FAD binding domain of DNA photolyase


FMO-like
PF00743.9
−381.6
Flavin-binding monooxygenase-like


Fe_bilin_red
PF05996.3
25
Ferredoxin-dependent bilin reductase


Fer2
PF00111.17
7
2Fe—2S iron-sulfur cluster binding





domain


Fer2_BFD
PF04324.5
25
BFD-like [2Fe—2S] binding domain


Flavin_Reduct
PF01613.8
−18
Flavin reductase like domain


Flavodoxin_1
PF00258.15
6.3
Flavodoxin


FtsJ
PF01728.9
−50.2
FtsJ-like methyltransferase


G-patch
PF01585.13
18.3
G-patch domain


GAP
PF01590.16
23
GAP domain


GATA
PF00320.17
28.5
GATA zinc finger


GATase_2
PF00310.11
−95.1
Glutamine amidotransferases class-II


GDC-P
PF02347.6
−306.2
Glycine cleavage system P-protein


GSHPx
PF00255.10
−16
Glutathione peroxidase


GSH_synth_ATP
PF03917.8
−129.9
Eukaryotic glutathione synthase, ATP





binding domain


GSH_synthase
PF03199.6
25
Eukaryotic glutathione synthase


GST_C
PF00043.15
22.3
Glutathione S-transferase, C-terminal





domain


GST_N
PF02798.10
14.6
Glutathione S-transferase, N-terminal





domain


GUN4
PF05419.3
25
GUN4-like


Gln-synt_C
PF00120.14
−124
Glutamine synthetase, catalytic domain


Gln-synt_N
PF03951.9
9
Glutamine synthetase, beta-Grasp domain


Globin
PF00042.12
−8.8
Globin


Glutaredoxin
PF00462.14
17.2
Glutaredoxin


Glyco_hydro_17
PF00332.9
−152.3
Glycosyl hydrolases family 17


Glyco_hydro_2
PF00703.11
−19.3
Glycosyl hydrolases family 2,





immunoglobulin-like beta-sandwich





domain


Glyco_hydro_2_C
PF02836.7
−127.7
Glycosyl hydrolases family 2, TIM barrel





domain


Glyco_hydro_2_N
PF02837.8
−46.9
Glycosyl hydrolases family 2, sugar





binding domain


Glyco_hydro_9
PF00759.9
−246.7
Glycosyl hydrolase family 9


Glyco_transf_5
PF08323.2
−114.9
Starch synthase catalytic domain


Glycos_transf_1
PF00534.10
−7.3
Glycosyl transferases group 1


Glyoxalase
PF00903.15
12.1
Glyoxalase/Bleomycin resistance





protein/Dioxygenase superfamily


HALZ
PF02183.8
17
Homeobox associated leucine zipper


HATPase_c
PF02518.16
22.4
Histidine kinase-, DNA gyrase B-, and





HSP90-like ATPase


HLH
PF00010.16
8.3
Helix-loop-helix DNA-binding domain


HSF_DNA-bind
PF00447.8
−70
HSF-type DNA-binding


Helicase_C
PF00271.21
2.1
Helicase conserved C-terminal domain


Hexokinase_1
PF00349.11
−110.3
Hexokinase


Hexokinase_2
PF03727.6
−131.3
Hexokinase


HisKA
PF00512.15
10.3
His Kinase A (phosphoacceptor) domain


Histone
PF00125.14
17.4
Core histone H2A/H2B/H3/H4


Histone HNS
PF00816.11
25
H-NS histone family


Homeobox
PF00046.19
−4.1
Homeobox domain


IGPD
PF00475.9
25
Imidazoleglycerol-phosphate dehydratase


Isoamylase_N
PF02922.8
−6.5
Isoamylase N-terminal domain


Isochorismatase
PF00857.11
−46
Isochorismatase family


KNOX1
PF03790.4
25
KNOX1 domain


KNOX2
PF03791.4
25
KNOX2 domain


KR
PF08659.1
−74.3
KR domain


Kelch_1
PF01344.15
7.8
Kelch motif


Kelch_2
PF07646.5
14
Kelch motif


LEA_3
PF03242.4
25
Late embryogenesis abundant protein


LRRNT_2
PF08263.3
18.6
Leucine rich repeat N-terminal domain


LRR_1
PF00560.23
7.7
Leucine Rich Repeat


LRR_2
PF07723.3
6
Leucine Rich Repeat


Lactamase_B
PF00753.17
24.6
Metallo-beta-lactamase superfamily


Ldh_1_C
PF02866.8
−13
lactate/malate dehydrogenase, alpha/beta





C-terminal domain


Ldh_1_N
PF00056.13
−31.3
lactate/malate dehydrogenase, NAD





binding domain


Lir1
PF07207.2
25
Light regulated protein Lir1


MBD
PF01429.10
12.9
Methyl-CpG binding domain


MEKHLA
PF08670.1
−59.7
MEKHLA domain


MFS_1
PF07690.6
23.5
Major Facilitator Superfamily


MGS
PF02142.12
3
MGS-like domain


Miro
PF08477.3
10.8
Miro-like protein


Mit_rib_S27
PF08293.2
25
Mitochondrial ribosomal subunit S27


Mo-co_dimer
PF03404.6
25
Mo-co oxidoreductase dimerisation





domain


Molybdop_Fe4S4
PF04879.6
13.6
Molybdopterin oxidoreductase Fe4S4





domain


Molybdopterin
PF00384.12
−50
Molybdopterin oxidoreductase


Molydop_binding
PF01568.11
1.1
Molydopterin dinucleotide binding





domain


MtN3_slv
PF03083.6
9.7
MtN3/saliva family


Myb_DNA-binding
PF00249.21
14
Myb-like DNA-binding domain


NAD_Gly3P_dh_C
PF07479.4
−50.8
NAD-dependent glycerol-3-phosphate





dehydrogenase C-terminus


NAD_Gly3P_dh_N
PF01210.13
−44
NAD-dependent glycerol-3-phosphate





dehydrogenase N-terminus


NAD_binding_1
PF00175.11
−3.9
Oxidoreductase NAD-binding domain


NAD_binding_2
PF03446.5
−63.5
NAD binding domain of 6-





phosphogluconate dehydrogenase


NIR_SIR
PF01077.12
−19.6
Nitrite and sulphite reductase 4Fe—4S





domain


NIR_SIR_ferr
PF03460.7
2.4
Nitrite/Sulfite reductase ferredoxin-like





half domain


NPH3
PF03000.5
25
NPH3 family


NTP_transferase
PF00483.13
−90.5
Nucleotidyl transferase


NUDIX
PF00293.18
0.2
NUDIX domain


Na_H_Exchanger
PF00999.11
−67.9
Sodium/hydrogen exchanger family


Na_H_antiport_1
PF06965.2
−270.8
Na+/H+ antiporter 1


Na_sulph_symp
PF00939.9
−259
Sodium: sulfate symporter transmembrane





region


Oxidored_molyb
PF00174.9
−39.6
Oxidoreductase molybdopterin binding





domain


P-II
PF00543.12
−29
Nitrogen regulatory protein P-II


PALP
PF00291.15
−70
Pyridoxal-phosphate dependent enzyme


PAS
PF00989.14
0
PAS fold


PAS_3
PF08447.1
13.4
PAS fold


PBP
PF01161.10
−20.6
Phosphatidylethanolamine-binding





protein


PFK
PF00365.10
−132
Phosphofructokinase


PGI
PF00342.9
−168.9
Phosphoglucose isomerase


PHP
PF02811.9
13.8
PHP domain


PLATZ
PF04640.4
20
PLATZ transcription factor


PMSR
PF01625.11
−62
Peptide methionine sulfoxide reductase


PP2C
PF00481.12
−44
Protein phosphatase 2C


PS1_PSAK
PF01241.9
25
Photosystem I psaG/psaK


PTPA
PF03095.5
−106
Phosphotyrosyl phosphate activator





(PTPA) protein


PTR2
PF00854.12
−50
POT family


PaO
PF08417.2
25
Pheophorbide a oxygenase


ParBc
PF02195.8
25
ParB-like nuclease domain


Peptidase_S10
PF00450.12
−198
Serine carboxypeptidase


Phi_1
PF04674.3
25
Phosphate-induced protein 1 conserved





region


Pkinase
PF00069.15
−70.3
Protein kinase domain


Pkinase_Tyr
PF07714.7
65
Protein tyrosine kinase


Pre-SET
PF05033.6
3.9
Pre-SET motif


Pribosyltran
PF00156.17
2
Phosphoribosyl transferase domain


Prismane
PF03063.10
−169.3
Prismane/CO dehydrogenase family


Proteasome
PF00227.16
−36.7
Proteasome A-type and B-type


PsbP
PF01789.7
25
PsbP


PsbQ
PF05757.2
25
Oxygen evolving enhancer protein 3





(PsbQ)


PsbW_2
PF07123.2
−25.8
Photosystem II reaction centre W protein





(PsbW)


Pyr_redox
PF00070.17
5
Pyridine nucleotide-disulphide





oxidoreductase


Pyr_redox_2
PF07992.4
−20
Pyridine nucleotide-disulphide





oxidoreductase


RGS
PF00615.9
10
Regulator of G protein signaling domain


RPE65
PF03055.6
−156.5
Retinal pigment epithelial membrane





protein


RRM_1
PF00076.12
17.7
RNA recognition motif, (a.k.a. RRM,





RBD, or RNP domain)


Radical_SAM
PF04055.11
8.5
Radical SAM superfamily


Ras
PF00071.12
−69.9
Ras family


Response_reg
PF00072.14
4
Response regulator receiver domain


Ribosomal_L10e
PF00826.8
25
Ribosomal L10


Ribosomal_S2
PF00318.10
−22
Ribosomal protein S2


Rieske
PF00355.16
−7
Rieske [2Fe—2S] domain


Rubredoxin
PF00301.10
12.1
Rubredoxin


S1
PF00575.13
16.8
S1 RNA binding domain


S10_plectin
PF03501.5
25
Plectin/S10 domain


SAM_decarbox
PF01536.7
−154
Adenosylmethionine decarboxylase


SAP18
PF06487.3
−49.7
Sin3 associated polypeptide p18 (SAP18)


SET
PF00856.18
23.5
SET domain


SHMT
PF00464.10
−238.5
Serine hydroxymethyltransferase


SNF5
PF04855.3
25
SNF5/SMARCB1/INI1


SOUL
PF04832.3
25
SOUL heme-binding protein


START
PF01852.10
−20.7
START domain


SWIM
PF04434.8
11
SWIM zinc finger


Sad1_UNC
PF07738.3
−20.4
Sad1/UNC-like C-terminal


Sina
PF03145.7
−48.4
Seven in absentia protein family


Spermine_synth
PF01564.7
−93.8
Spermine/spermidine synthase


Sterol_desat
PF01598.8
−13
Sterol desaturase


Sugar_tr
PF00083.14
−85
Sugar (and other) transporter


TPP_enzyme_C
PF02775.11
19.7
Thiamine pyrophosphate enzyme, C-





terminal TPP binding domain


TPP_enzyme_M
PF00205.12
−8.1
Thiamine pyrophosphate enzyme, central





domain


TPP_enzyme_N
PF02776.8
−70
Thiamine pyrophosphate enzyme, N-





terminal TPP binding domain


TPR_1
PF00515.18
7.7
Tetratricopeptide repeat


TPR_2
PF07719.7
20.1
Tetratricopeptide repeat


TPR_4
PF07721.5
15
Tetratricopeptide repeat


TPT
PF03151.7
−15.3
Triose-phosphate Transporter family


TP_methylase
PF00590.10
−38
Tetrapyrrole (Corrin/Porphyrin)





Methylases


Thi4
PF01946.7
−65.1
Thi4 family


Thioredoxin
PF00085.10
−25.7
Thioredoxin


Tim17
PF02466.9
2.7
Tim17/Tim22/Tim23 family


Tryp_alpha_amyl
PF00234.12
−4
Protease inhibitor/seed storage/LTP





family


UAA
PF08449.2
−146.2
UAA transporter family


VPS28
PF03997.3
25
VPS28 protein


Vps4_C
PF09336.1
−4.6
Vps4 C terminal oligomerisation domain


WD40
PF00400.22
21.5
WD domain, G-beta repeat


Whirly
PF08536.2
25
Whirly transcription factor


YABBY
PF04690.4
25
YABBY protein


YDG_SRA
PF02182.8
25
YDG/SRA domain


adh_short
PF00106.15
−40.2
short chain dehydrogenase


bZIP_1
PF00170.11
24.5
bZIP transcription factor


bZIP_2
PF07716.5
15
Basic region leucine zipper


eIF-1a
PF01176.9
20
Translation initiation factor 1A/IF-1


efhand
PF00036.22
21.7
EF hand


mTERF
PF02536.5
−60
mTERF


p450
PF00067.12
−105
Cytochrome P450


peroxidase
PF00141.13
−10
Peroxidase


tRNA-synt_2
PF00152.11
−168.2
tRNA synthetases class II (D, K and N)


tRNA_anti
PF01336.15
8
OB-fold nucleic acid binding domain


ubiquitin
PF00240.13
19.4
Ubiquitin family


zf-C3HC4
PF00097.15
16
Zinc finger, C3HC4 type (RING finger)


zf-CCCH
PF00642.15
0
Zinc finger C-x8-C-x5-C-x3-H type (and





similar)


zf-Dof
PF02701.6
25
Dof domain, zinc finger


zf-UBR
PF02207.10
25
Putative zinc finger in N-recognin (UBR





box)









Example 9
Selection of Transgenic Plants with Enhanced Agronomic Trait(s)

This example illustrates the preparation and identification by selection of transgenic seeds and plants derived from transgenic plant cells of this invention where the plants and seed are identified by screening for a transgenic plant having an enhanced agronomic trait imparted by expression of a protein selected from the group including the homologous proteins identified in Example 6. Transgenic plant cells of corn, soybean, cotton, canola, alfalfa, wheat and rice are transformed with recombinant DNA for expressing each of the homologs identified in Example 6. Plants are regenerated from the transformed plant cells and used to produce progeny plants and seed that are screened for enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Plants are identified exhibiting enhanced traits imparted by expression of the homologous proteins.

Claims
  • 1. A plant cell nucleus with stably integrated, recombinant DNA construct, wherein said recombinant DNA construct comprises a promoter that is functional in a plant cell and that is operably linked to a DNA segment encoding a protein comprising an amino acid sequence of SEQ ID NO: 309; and wherein said recombinant DNA construct is stably integrated into a chromosome in said plant cell nucleus which is selected by screening a population of transgenic plants that have said recombinant DNA construct and an enhanced trait as compared to control plants that do not have said recombinant DNA construct in their nuclei; andwherein said enhanced trait is selected from group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, enhanced heat tolerance, enhanced high salinity tolerance, enhanced shade tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.
  • 2. A recombinant DNA construct comprising a promoter that is functional in a plant cell and that is operably linked to a DNA segment that encodes: a. at least one protein having an amino acid sequence comprising a Pfam domain module selected from the group consisting of zf-CCCH, PALP, GAF::HisKA::HATPase_c, TPR—2::TPR—1::TPR—2::TPR—1::TPR—4::TPR—2::TPR—1, efhand::efhand, Spermine_synth, ELFV_dehydrog_N::ELFV_dehydrog, PFK, PAS—3::PAS—3::Pkinase, S1, GDC-P, SWIM, B12-binding::Radical_SAM, S1, LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1, Chloroa_b-bind, LRRNT—2::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::Pkinase, WD40::WD40, YABBY, Ldh—1_N::Ldh—1_C, Sina, Na_H_antiport—1, ParBc, YABBY, Histone, Fe_bilin_red, Tryp_alpha_amyl, Pyr_redox—2::Thioredoxin, E2F_TDP, CN_hydrolase, YDG_SRA::Pre-SET::SET, APC8::TPR—1::TPR—1::TPR—1, Ras, tRNA_anti::tRNA-synt—2, Auxin_inducible, PGI, S1, Chloroa_b-bind, Bac_globin, Glyco_hydro—17, MGS, Spermine_synth, LRRNT—2::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::Pkinase, Aa_trans, LRRNT—2::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::Pkinase_Tyr, Gln-synt_N::Gln-synt_C, SAP18, TPP_enzyme_N::TPP_enzyme_M::TPP_enzyme_C, zf-C3HC4, elF-1a, RPE65, PBP, Pkinase, AA_permease, F-box::LRR—1::LRR—2, zf-CCCH::zf-CCCH, Lactamase_B::Flavodoxin—1::Flavin_Reduct, Bac_globin, DSPc, adh_short, Tim17, Oxidored_molyb::Mo-co_dimer:Cyt-b5::FAD_binding—6::NAD_binding—1, ubiquitin::ubiquitin::ubiquitin::ubiquitin, MBD, CXC::CXC, HSF_DNA-bind, Spermine_synth, AP2, Peptidase_S10, PALP, EIN3, Gln-synt_N::Gln-synt_C, 2OG-FeII_Oxy, Glyco_hydro—9, GDC-P, B3, PTPA, Acyltransferase, Isochorismatase, FMO-like, Molybdop_Fe4S4::Molybdopterin::Molydop_binding::Fer2_BFD, Lir1, Prismane, Fer2, DEAD::Helicase_C, Molybdop_Fe4S4::Molybdopterin::Molydop_binding::Fer2_BFD, KNOX1::KNOX2::ELK, Glyoxalase, Sad1_UNC, Bac_globin, VPS28, PP2C, Pkinase::efhand::efhand::efhand, LEA—3, Peptidase_S10, Pkinase, CBFD_NFYB_HMF, Gln-synt_N::Gln-synt_C, Pyr_redox—2::Fer2_BFD::NIR_SIR_ferr::NIR_SIR, NUDIX::NUDIX, FAD_binding—3, GST_N::GST_C, SAM_decarbox, Acyltransferase, NTP_transferase, G-patch, 2OG-FeII_Oxy, Gln-synt_N::Gln-synt_C, AAA::Vps4_C, Histone, Pkinase, TPR—1, F-box::Kelch—1::Kelch—1, Spermine_synth, Bac_globin, Bac_globin, zf-UBR, Homeobox::HALZ, Whirly, NAD_binding—1, PTR2, EIN3, 4HBT, adh_short, 2OG-FeII_Oxy, P-II, Myb_DNA-binding::Myb_DNA-binding, DAGK_cat, AP2, MFS—1, Chloroa_b-bind, DUF716, zf-D of, CCT, Homeobox::HALZ, Histone, 2OG-FeII_Oxy, Globin, Pyr_redox—2::Fer2_BFD::NIR_SIR_ferr::NIR_SIR, Whirly, PsbP, bZIP—1, LRRNT 2::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::Pkinase_Tyr, Phi—1, BURP, Sterol_desat, DSPc, SNF5, Acyltransferase, GATase—2::Asn_synthase, adh_short, Homeobox::START, Pkinase, ParBc, SOUL, DNA_photolyase::FAD_binding—7, Pkinase_Tyr, NAD_Gly3P_dh_N::NAD_Gly3P_dh_C, Na_H_Exchanger, peroxidase, Oxidored_molyb::Mo-co_dimer:Cyt-b5::FAD_binding—6::NAD_binding—1, Hexokinase—1::Hexokinase—2, DUF716, S10_plectin, Thi4, p450, CCT, adh_short, PSI_PSAK, DUF640, Thioredoxin, Globin, Ank::Pkinase, DAGAT, RPE65, Ank::Pkinase, GSHPx, Gln-synt_N::Gln-synt_C, MtN3_slv::MtN3_slv, Allene_ox_cyc, IGPD, MBD, CorA, Response_reg, Histone, AAA, Ribosomal_L10e, Pkinase, DUF26::DUF26::Pkinase, p450, mTERF, AA_kinase, PBP, GUN4, Lactamase_B::Flavodoxin—1::Rubredoxin, C2, RRM—1::RRM—1, Histone, Alpha-amylase, HLH, Thioredoxin, Histone_HNS, Myb_DNA-binding::Myb_DNA-binding, Cytochrom_C552, AP2::AP2, MtN3_slv::MtN3_slv, SHMT, ParBc, Mit_rib_S27, Ribosomal_S2, KNOX1::KNOX2::ELK, MFS—1, Glyco_transf—5::Glycos_transf—1, Cellulase, Ribosomal_L10e, Spermine_synth, Glyco_hydro—2_N::Glyco_hydro—2::Glyco_hydro—2_C, TP_methylase, AP2::AP2, Histone, Response_reg::CCT, Histone_HNS, DUF1716, p450, GATA, Pkinase, Sugar_tr, Aa_trans, Pribosyltran, Ribosomal_L10e, HLH, PMSR, DnaJ::DnaJ_CXXCXGXG::DnaJ_C, DUF1005, Glyco_transf—5::Glycos_transf—1, Spermine_synth, S1::EIF—2_alpha, RGS, Na_sulph_symp, S1, MtN3_slv::MtN3_slv, Lactamase_B::Flavin_Reduct, LRRNT—2::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::LRR—1::Pkinase, Chloroa_b-bind, PTR2, Agglutinin, PLATZ, NPH3, Auxin_inducible, PTR2, GAF::HisKA::Response_reg, PsbQ, GSH_synth_ATP, GATase—2::Asn_synthase, PHP, FtsJ, DUF6::TPT, Proteasome, PsbW—2, Glyco_hydro—9, NAD_binding—2::6 PGD, S1::EIF—2_alpha, Homeobox::START::MEKHLA, S1, Isoamylase_N::Alpha-amylase, E2F_TDP, and Rieske::PaO;b. a protein comprising an amino acid sequence with at least 90% identity to a consensus amino acid sequence selected from the group consisting of SEQ ID NO: 30526 through 30550;c. a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs 352, 493, 533 and 544, and homologs thereof listed in Table 8;d. a protein having an amino acid sequence having at least 70% identity to an amino acid sequence selected from the group consisting of SEQ ID NOs 320 and 383; ore. a protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 299 through SEQ ID NO: 30468;and wherein said recombinant DNA construct is stably integrated into a chromosome in a plant cell nucleus which is selected by screening a population of transgenic plants that have said recombinant DNA construct and an enhanced trait as compared to control plants that do not have said recombinant DNA construct in their nuclei; and wherein said enhanced trait is selected from group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, enhanced heat tolerance, enhanced high salinity tolerance, enhanced shade tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil.
  • 3. A transgenic plant cell nucleus comprising a recombinant DNA construct of claim 2.
  • 4. A transgenic plant cell having a plant cell nucleus of claim 3.
  • 5. The transgenic plant cell of claim 4 wherein said transgenic plant cell is homozygous for said recombinant DNA construct.
  • 6. The transgenic plant cell of claim 4 further comprising DNA expressing a protein that provides tolerance from exposure to an herbicide applied at levels that are lethal to a wild type of said plant cell.
  • 7. The transgenic plant cell of claim 5 wherein said herbicide is a glyphosate, dicamba, or glufosinate compound.
  • 8. A transgenic plant comprising a plurality of plant cells of claim 4.
  • 9. The transgenic plant of claim 8 wherein said transgenic plant is homozygous for said recombinant DNA construct.
  • 10. A transgenic seed comprising a recombinant DNA construct of claim 2.
  • 11. The transgenic seed of claim 10 from a corn, soybean, cotton, canola, alfalfa, wheat or rice plant.
  • 12. A transgenic pollen grain comprising a recombinant DNA construct of claim 2.
  • 13. A method for manufacturing transgenic seeds that can be used to produce a crop of transgenic plants with an enhanced trait resulting from expression of a DNA segment in a plant cell nucleus comprising a recombinant DNA construct of claim 2, wherein said method comprises: (a) screening a population of plants for said enhanced trait and said recombinant DNA construct, wherein individual plants in said population can exhibit said trait at a level less than, essentially the same as or greater than the level that said trait is exhibited in control plants which do not contain said recombinant DNA construct, wherein said enhanced trait is selected from the group of enhanced traits consisting of enhanced water use efficiency, enhanced cold tolerance, enhanced heat tolerance, enhanced high salinity tolerance, enhanced shade tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil;(b) selecting from said population one or more plants that exhibit said trait at a level greater than the level that said trait is exhibited in control plants; and(c) collecting seeds from selected plant selected from step b.
  • 14. The method of claim 13, wherein said method further comprises: (a) verifying that said recombinant DNA construct is stably integrated in said selected plants; and(b) analyzing tissue of said selected plant to determine the expression of a protein having the function of a protein having an amino acid sequence selected from the group consisting of one of SEQ ID NO: 299 through SEQ ID NO: 30468.
  • 15. The method of claim 14 wherein said seed is corn, soybean, cotton, alfalfa, canola wheat or rice seed.
  • 16. A method of producing hybrid corn seed comprising: (a) acquiring hybrid corn seed from a herbicide tolerant corn plant which also has a stably-integrated, recombinant DNA construct of claim 2;(b) producing corn plants from said hybrid corn seed, wherein a fraction of the plants produced from said hybrid corn seed is homozygous for said recombinant DNA construct, a fraction of the plants produced from said hybrid corn seed is hemizygous for said recombinant DNA construct, and a fraction of the plants produced from said hybrid corn seed has none of said recombinant DNA construct;(c) selecting corn plants which are homozygous or hemizygous for said recombinant DNA construct by treating with an herbicide;(d) collecting seeds from herbicide-treated-surviving corn plants and planting said seed to produce further progeny corn plants;(e) repeating steps (c) and (d) at least once to produce an inbred corn line; and(f) crossing said inbred corn line with a second corn line to produce hybrid corn seed.
CROSS REFERENCE TO RELATED APPLICATIONS

This application claims benefit under 35USC §119(e) of U.S. provisional application Ser. No. 60/958,909, filed Jul. 10, 2007 herein incorporated by reference in its entirety.

Provisional Applications (1)
Number Date Country
60958909 Jul 2007 US
Continuations (1)
Number Date Country
Parent 12218251 Jul 2008 US
Child 13815411 US