Patent Application
20220257639
This invention pertains in general to treatment of patients under antiplatelet medication using agents that modulate human blood platelet activation and aggregation. Especially patients experiencing bleeding complications during/after surgery. Furthermore, the present invention pertains to a platelet endothelial receptor 1 (PEAR1) agonist, such as natural fucoidans, synthesized fucoidan-mimetic glycopolymers such as sulfated α-L-fucoside-pendant glycopolymer, or dextran sulfate for such use.
Human blood platelets, also colloquially designated thrombocytes, patrol the blood vessels as sentinels of vascular integrity. Platelets play a pivotal role in physiological haemostasis by adhering to the injured vessel wall, aggregation, propagation of coagulation, and thrombus formation; the latter which is also referred to as a “blood clot”. Further, platelets are also subsequently involved in fibrinolysis (decomposition of the thrombus) and the repair of the vessel wall, thereby restoring blood flow and vascular integrity.
Under pathophysiological conditions such as atherosclerosis, the rupture of an atherosclerotic plaque can lead to inappropriate platelet aggregation and thrombus formation that can cause vascular occlusions resulting in acute myocardial infarction (AMI) or stroke, which are, according to the World Health Organization (WHO), the worldwide leading causes for death.
The formation of a thrombus, simplified spoken, involves two major components: Activated platelets and soluble blood constituents, the latter which are designated “coagulation factors” and in their entity form the “coagulation cascade”. Activated platelets, besides releasing autocrine feed-back mediators (such as thromboxane A2 (TXA2) and adenosine diphosphate (ADP)) and building up a loose fibrinogen-bridged aggregate, change the composition and electric charge of their outer membrane into a so-called “procoagulant surface”. To this surface coagulation factors bind and are transformed into their active forms, finally funneling into the conversion of fibrinogen into fibrin, thereby turning the loose platelet-aggregate into a stable thrombus.
In this respect, antithrombotic medication is separated into two major groups of pharmaceuticals. One group, the anticoagulants, targets the components of the coagulation cascade, such a heparins or vitamin K antagonists. The other group, the antiplatelet drugs, interferes directly with the initial activation of platelets. As such, anticoagulants allow for platelet aggregation but impede coagulation, whereas antiplatelet drugs hinder initial platelet activation and aggregation, and by that likewise coagulation. Anticoagulants are mostly prescribed to patients showing clinical indications which may lead to a future thrombotic event. Antiplatelet drugs, in contrast, are immediately and in a high dosage administered to patients experiencing an AMI or stroke. Patients surviving such events must often receive a lifetime treatment with antiplatelet drugs in order to prevent the recurrence of such life-threatening complications. Along this line, the combination of drugs which interfere with TXA2 synthesis and signalling (such as aspirin) and antagonists of the P2Y12 receptor for ADP (such as clopidogrel, cangrelor, or ticagrelor) represents today's “gold standard” for dual antiplatelet therapy (DAPT) to treat acute events as well as their recurrence. If such patients require surgery or dental work that can be planned in advance, a controlled temporary discontinuation of medication is the preferred practice today. However, when patients under antiplatelet medication are in vital need of instant/acute surgery bleeding complications are unavoidable.
On rare occasions, however, patients may be misdiagnosed as experiencing an AMI or stroke because of the shown symptoms and comparatively high incidence of such events and will therefore been instantly administered an ultra-high dose of a fast acting P2Y12 receptor antagonist, such as clopidogrel, cangrelor, or ticagrelor. When patients are subsequently diagnosed as experiencing for instance an acute aortic dissection (AD) instead of an AMI or stroke, immediate surgery is inevitable. The surgical method of choice is that the aorta is divided and a surgical interposition graft is sewn in. At sites of the joining between the aorta and the graft, as well as at the stitching, extended bleedings occur due to the lack of platelet aggregation, coagulation, and plug formation, as the direct result of the initial admission of an ultra-high dose of a P2Y12 receptor antagonist.
Today, no solutions exist to effectively stop bleedings under above mentioned circumstances. Existing products are topical haemostatics, sealants, and adhesives. Hemostats can clot blood. Sealants can create sealing barriers. Adhesives bond tissue together. Collagen, gelatin, and cellulose are haemostatic agents. TachoSil® is a development of TachoComb® and TachoComb®H. TachoComb is made with equine collagen, bovine thrombin, bovine aprotinin, and human fibrinogen. However, none of these products can effectively solve the bleeding complications during surgery caused by P2Y12 receptor-blocking or DAPT medication.
The lack of methods or products to solve the complications caused by P2Y12 receptor-antagonizing medication or DAPT during acute surgery often cause that patients may have to suffer several days with open chest wounds and receive a multitude of blood transfusions, which both bear the additional risk of severe infections until bleeding has stopped and before the surgeon can close the wound.
As such, there is a need for treatment strategies to tackle bleeding complications in patients under antiplatelet medication, especially for those who are in vital need of immediate surgery.
Accordingly, the present invention preferably seeks to mitigate, alleviate or eliminate one or more of the above-identified deficiencies in the art and disadvantages singly or in any combination and solves at least the above-mentioned problems by providing a platelet endothelial aggregation receptor 1 (PEAR1) agonist for use in the treatment of bleeding in a patient, wherein the patient is under antiplatelet medication.
Provided is also a topical haemostatic, a sealant, or an adhesive, to be locally applied during surgery, wherein the topical haemostatic, a sealant, or an adhesive comprises a platelet endothelial aggregation receptor 1 (PEAR1) agonist, for treating bleeding in a patient under antiplatelet medication.
These and other aspects, features and advantages of which the invention is capable of will be apparent and elucidated from the following description of embodiments of the present invention, reference being made to the accompanying drawings, in which
The following description focuses on an embodiment of the present invention applicable to treatment strategies for patients who received antiplatelet medication. Especially to treatment of patients who suffer from bleeding complications during/after surgery.
In the course of physiological primary haemostasis, blood platelets instantly adhere to the denuded sub-endothelium at the site of vascular injury, become activated and release autocrine mediators such as thromboxane A2 (TXA2) and adenosine diphosphate (ADP), enable the conformation of the fibrinogen-receptor integrin αIIbβ3 into an activated state, and finally aggregate to build a fibrinogen-bridged plug. For TXA2 the thromboxane/prostanoid receptor-α (TPα) is the predominant isoform on platelets and, like the receptors for thrombin (PAR-1 and PAR-4), couples to Gα12/13 and Gαq. For ADP the Gαq-coupled P2Y1 receptors and Gαi2-coupled P2Y12 receptors have been identified on platelets. Signalling via the (β/γ-subunit of Gαi is known to be associated with the amplification of granule secretion as well as the activation of integrin αIIaβ3 (known as ‘inside-out’ signalling) whereas the α-subunit down-regulates adenylyl cyclase (AC) and therefore cyclic adenosine monophosphate (cAMP) levels, all which is pivotal for full and sustained platelet activation and aggregation.
Human platelet aggregation induced by a physiological agonist, as exemplified in
Whereas platelet activation and aggregation induced by primary platelet agonists to various degrees depend on intermediate TXA2 generation and autocrine signalling, all known physiological platelet agonists so far, regardless their initial signaling cascades employed, crucially depend on ADP release and subsequent P2Y12 signaling. The combined inhibition of TXA2/TPα-signalling by e.g. aspirin and ADP/P2Y12-signalling by e.g. ticagrelor represents today's “gold standard” for anti-thrombotic therapy. Most, if not all, patients who experienced an AMI or stroke are under this dual anti-platelet therapeutic regime (DAPT) to prevent the recurrence of a thrombotic event.
Thus, for patients under antiplatelet therapies, the first phase of platelet aggregation may occur, but, as illustrated in
In patients receiving antiplatelet medication platelet aggregation is prevented or reversible. When these patients require instant or acute surgery, bleeding complications to various extents are inevitable.
Fucoidans, polysulfated fucose polysaccharides of varying chain-length from natural sources have so far been suggested to be used in a clinical setting for having either pro-coagulatory or anti-coagulatory properties, depending on the observations reported or stated. However, a precise mode of action on molecular levels has been elusive. In WO 2008103234, it was suggested that purified fucodians may be used as an anti-coagulant to subjects with pro-thrombotic conditions like deep vein thrombosis, arterial thrombosis, and other cardiovascular diseases.
The effects of fucoidans on blood platelet activation and aggregation are far less developed. It has previously been reported that natural fucoidan isolated from F. vesiculosus induces human and murine platelet activation and aggregation via the C-type Lectin-like receptor 2 (Clec-2). In studies related to this disclosure, these findings could not be reproduced, and the platelet endothelial receptor 1 (PEAR1) was instead identified as the main receptor provoking human platelet activation and aggregation by natural fucoidan as well as synthetic sulfated α-L-fucoside-pendant glycopolymers [1]. Previous to identifying the involvement of PEAR-1, it was shown that synthetic sulfated α-L-fucoside-pendant glycopolymers are potent agonists of human platelet activation and aggregation [2, 3]. However, PEAR1 has been reported to be likewise activated by dextran sulfate; the latter which was therefor included in studies related to this disclosure as a positive control for PEAR1 induced signalling. In Kauskot et.al. [4], a PEAR1 extracellular domain protein (abbreviated PEAR1-EC) was shown to induce aggregation of washed (isolated) human platelets in a concentration-dependent fashion, however, nothing pointed to possible clinical applications of a PEAR1-EC.
Along this line, the endogenous ligand for PEAR1 in humans as well as other species still remains to be elucidated.
With respect to the two phases of platelet aggregation mentioned above and illustrated in
Further, in the presence of receptor antagonists which interfere with TXA2/TPα and ADP/P2Y12 signalling (dual inhibition) primary and reversible platelet aggregation caused by collagen and the thrombin-mimetic PAR-1 activating hexapeptide SFLLRN has been observed to various extends, and, as expected, a lack of the second and irreversible phase.
In sharp contrast, and most noteworthy, as disclosed herein, it was shown that natural fucoidan (from F. vesiculosus), the synthetic sulfated α-L-fucoside-pendant glycopolymer with a chain length of 34 monomers (C34), as well as dextran sulfate provoke the first phase of platelet aggregation, which to our very surprise remained irreversible and sustained in the presence of both TPα and P2Y12 antagonists (dual inhibition; DAPT).
In
With other words, it was found that PEAR1 receptor agonists appear to be able to induce approximately half-maximal and sustained aggregation of human platelets in plasma despite blocking P2Y12 receptor signalling, TPα receptor signalling, or both P2Y12 and TPα receptor signalling (dual inhibition; dual antiplatelet therapy; DAPT).
As such, it was found that for patients receiving mono- or dual antiplatelet medication, the use of a platelet endothelial aggregation receptor 1 (PEAR1) agonist (here fucoidans, synthetic sulfated α-L-fucoside-pendant glycopolymers, and dextran sulfate) could result in approximately half-maximal and sustained platelet aggregation. Importantly, for patients receiving antiplatelet therapies, a platelet endothelial aggregation receptor 1 (PEAR1) agonist treatment would thus be directly linked to lessening and minimizing bleeding complications.
In one embodiment, a platelet endothelial aggregation receptor 1 (PEAR1) agonist for use in the treatment of bleeding in a patient wherein the patient is under antiplatelet medication.
As such, platelet endothelial aggregation receptor 1 (PEAR1) agonists present a viable treatment option to tackle bleeding complications in a large number of patients under single or dual anti-platelet therapeutic regime.
Such treatment is normally administered at the point of bleeding for local effect, such as through topical administration of the platelet endothelial aggregation receptor 1 (PEAR1) agonists at the point of trauma or bleeding.
In one embodiment, the administration is topical administration.
In one embodiment, the administration is local administration at the point of trauma or bleeding.
For larger injuries, the extent of the injury is preferably assessed to provide information for the best treatment of the injury. In severe cases of bleeding, the administration of the platelet endothelial aggregation receptor 1 (PEAR1) agonists may be combined with other blood stopping measures, such as stiching or the temporary use of a tourniquet.
There are several different antiplatelet drugs such as acetylsalicylic acid (aspirin), nonsteroidal anti-inflammatory drugs (NSAIDs), dipyridamole, antagonists of the P2Y12 adenosine diphosphate (ADP) receptor, and inhibitors of integrin αIIbβ3 function. The combined inhibition of TXA2/TPα-signaling by e.g. aspirin and ADP/P2Y12-signalling by e.g. ticagrelor is the standard anti-thrombotic therapy.
In one embodiment, the antiplatelet medication or antiplatelet therapy comprises one or several drugs inhibiting TXA2 synthesis and/or being antagonists for the TPα receptor, and/or one or several drugs that are ADP receptor inhibitors and/or being antagonists for the P2Y12 receptor.
In one embodiment, the antiplatelet medication or antiplatelet therapy comprises one or several drugs selected from acetylsalicylic acid, triflusal, and terutroban, and/or one or several drugs selected from ticlopidine, clopidogrel, cangrelor, prasugrel, elinogrel, and ticagrelor.
Patient groups under such medications are extensive, and include stroke patients, patients with heart disease and patients who have undergone surgical procedures.
In one embodiment, the patient suffers or has suffered from a condition selected from the group consisting of stroke with or without atrial fibrillation, heart surgery, prosthetic replacement of heart valve, coronary heart disease such as stable angina, unstable angina and heart attack, patients with coronary stent, peripheral vascular disease/peripheral arterial disease and apical/ventricular/mural thrombus, coronary artery disease, heart attack, stent or coronary artery bypass graft surgery (CABG).
Antiplatelet treatment has become more and more effective, and modern antiplatelet therapies can maintain their effects for as long as 72 hours after administration. Although this is a clear advantage in terms of convenience for a patient under livelong treatment, it represents a major risk if a patient is in need of unplanned immediate surgery.
It is crucial to be able to stop bleeding during or after surgery where the patient is under antiplatelet therapy, and especially when the patient has received a high and preventative dose of the antiplatelet drug, such as within 72, 48, 24, 12, such as 6, 5, 4, 3, 2, or 1 hour. Such bleeding complications include patients receiving continuous single or dual antiplatelet medication and require immediate surgery or dental work.
In one embodiment, the bleeding is acute bleeding.
In one embodiment, the bleeding is caused by a surgical procedure.
In one embodiment, the antiplatelet medication or antiplatelet therapy has been administered to the patient within 72, 48, 24, 12, 6, 5, 4, 3, 2, or 1 hour.
One specific example of such a patient group is associated with acute aortic dissection (AD), a catastrophic manifestation in which the inner layer of the aorta tears. Blood surges through the tear from the true lumen into a false lumen, causing the inner and middle layers of the aorta to separate (to dissect), as can be seen in
The deeper the dissection and the further the ballooning develops the more tissues are affected by malperfusion (
A Swedish population-based study of 14.229 individuals, which included a high rate of post-mortem examinations, revealed an incidence for AD of 3.4 per 100.000 people per year. Including the estimated number of unreported cases, the International Registry of Acute Aortic Dissections (IRAD) assesses incidences for AD per 100.000 people of 16 in males and 7.9 in females, respectively, with a mean age of presentation of 63 years. By comparison, in this age cohort, the incidence of experiencing an acute myocardial infarction (AMI) is approximately 400 in 100.000 inhabitants per year in Sweden (www.socialstyrelse.se).
In this respect, symptoms of AD are largely similar to those of a severe thrombotic event, such as a AMI or stroke, and may include, for instance, sudden severe chest or upper back pain, shortness of breath, sudden difficulty speaking, loss of vision, or weakness or paralysis of one side of the body. Because of these similarities and the probability of incidence, when patients are admitted to the emergency hospitalisation showing one or more of the above-mentioned symptoms, they are immediately administered a high and preventative dose of an antiplatelet drug, such as clopidogrel, cangrelor, or ticagrelor, which targets the platelet P2Y12/ADP receptors. In one embodiment, the antiplatelet medication or antiplatelet therapy comprises administering the drugs clopidogrel, cangrelor, and/or ticagrelor.
In one embodiment, the antiplatelet medication or antiplatelet therapy is a dual anti-platelet therapy (DAPT). Such therapy can be 60-90 mg ticagrelor twice daily combined with 70-100 mg aspirin.
When patients are subsequently diagnosed as experiencing an AD instead of an AMI or stroke, immediate surgery is inevitable. Normally, this entails an open thoracic aortic replacement, where the aorta is divided and a surgical Dacron interposition graft is sewn in. Surgical access is via a large cut in the left side of the chest.
At sites of the joining between the aorta and the graft, as well as at the stitching, extended bleedings occur due to the lack of platelet aggregation as the direct result of the initial admission of an ultra-high dose of a P2Y12 receptor antagonist such as clopidogrel, cangrelor, or ticagrelor.
Until bleedings stop a patient may require infusion of as many as 30 blood bags and/or spend as long as two days with an open chest in the intensive care unit. Notwithstanding the economic aspect, every blood transfusion and every minute with an opened thorax substantially increase the risk of severe infections adding to the already highly critical situation of the patient.
Thus, for patients with acute aortic dissection (AD) subjected to immediate surgery after receiving an ultra-high dose of anti-P2Y12 platelet medication, bleeding complications can be coped with and stopped using a platelet endothelial aggregation receptor 1 (PEAR1) agonist.
In one embodiment, the patient suffers from acute aortic dissection (AD).
In one embodiment, the patient has received a high dose of a P2Y12 receptor antagonist, such as ticagrelor at 180-480 mg.
In one embodiment, the patient has been initially misdiagnosed as experiencing an AMI or stroke and therefore has received a high dose of a P2Y12 receptor antagonist, such as ticagrelor at 180-480 mg.
In one embodiment, the platelet endothelial aggregation receptor 1 (PEAR1) agonist is a polysulfated polysaccharide and/or a sulfated glycopolymer. As demonstrated by the data in this application, all these agonists provoke platelet aggregation.
The sustained platelet aggregation for a sulfated glycopolymer (sulfated α-L-fucoside-pendant glycopolymer), natural fucoidan from F. vesicolosus, and dextran sulfate is shown in
The chemical composition of natural fucoidans is extremely variable depending on eco-physiological parameters. The first structure was elucidated in 1950 from a fucoidan extracted from F. vesiculosus. Subsequently it has been determined that fucoidans were composed of 50%-90% of L-fucose, 35%-45% of sulfate and less than 8% of uronic acid with a linear backbone based on an α(1→2)-glycosidic linkage of O-4 sulfated L-fucose and some oses like galactose, mannose, xylose, and glucose. A revised structure of a commercial fucoidan from F. vesiculosus has later been published demonstrating that it mainly consitsts of an α(1→3)-L-fucose linear backbone with sulfate substitution at O-4 and some α-L-fucose branched at O-4 or O-2.
In one embodiment, the polysulfated polysaccharide is a fucoidan and/or a dextran sulfate.
In one embodiment, the polysulfated polysaccharide is a fucoidan.
In one embodiment, the fucodian is extracted from brown algae or brown seaweed.
In one embodiment, the fucodian is extracted from F. vesiculosus.
However, the heterogeneous structure of natural fucodians may pose a problem in such applications. Also, the degree of sulfation may differ between purified batches, which may directly relate to its effect. Being purified, it is hard to get a completely pure sample, and this potential batch diversity renders dosing comlicated.
As such, it was recognized that synthesized fucoidan-mimetic glycopolymers, such as a sulfated α-L-fucoside-pendant glycopolymer could present several advantages.
In one embodiment, the polysulfated glycopolymer is a sulfated α-L-fucoside-pendant glycopolymer.
Fucoidan-mimetic glycopolymers can be synthezised in several different ways, such as by cyanoxyl-mediated free-radical polymerization, thiol-mediated chain transfer free-radical polymerization techniques, or RAFT polymerization. In the invention, it was found that RAFT polymerization provides unbranched polysaccharide chains and offers good control of the polymer chain length.
In one embodiment, the sulfated α-L-fucoside-pendant glycopolymer comprises an unbranched chain of pendant carbohydrates.
In the invention, artificially synthesized sulfated fucose glycopolymers (fucoidan-mimetic sulfated glucopolymers), of non-human and non-animal origin, was compared to purified fucodian. It was found that the synthezied sulfated glycopolymers evoke the same sustained effect on platelet aggregation as was observed as for the purified fucoidan (
The chain length was selected to provide sufficient biological activity at the applied concentration, while still being short enough to ensure synthesis of a homogeneous fraction. Further, the chain length appears appropriate to synthesize or couple the glycopolymer to a linker/adaptor protein.
As shown in
In one embodiment, the unbranched chain of pendant carbohydrates contains at least 10 monosaccharide units, such as at least 13 monosaccharide units.
In one embodiment, the unbranched chain of pendant carbohydrates contains from 10 to 500, such as 10 to 350 monosaccharide units, such as 13 to 329 monosaccharide units.
In one embodiment, the unbranched chain of pendant carbohydrates contains 13, 34 or 329 monosaccharide units.
The artificially synthesized sulfated glycopolymer is a sulfated α-L-fucoside-pendant poly(methacryl amide). The end product is exemplified in the formula 1 below:
In one embodiment, the sulfated glycopolymer is of the general formula 1, wherein R=SO3Na. In one further embodiment, n=10 to 500, such as 13 to 329, such as 13, 34 or 329.
In one embodiment, the sulfated glycopolymer is a sulfated α-L-fucoside-pendant poly(methacryl amide).
To furnish the fucoidan-mimetic sulfated glycopolymer, all glycopolymers are O-sulfated with sulfate esters. In the invention, a high degree of sulfatation was preferred, such as over 50% of sulfatation, such as more than 60%, more than 70%, more than 80% or more than 90% degree of sulfatation.
In one embodiment, the fucoidan or sulfated glycopolymer is sulfated to a degree of at least 50%, such as at least 60%, preferrably to at least 75%, such as at least 85%. In one embodiment, the fucoidan or sulfated glycopolymer is sulfated to a degree of 50% to 100%, such as 75% to 95%, such as 85% to 90%.
The synthesized sulfated glycopolymer present several advantages. They can be highly purified, and the length can be controlled as well as the degree of sulfatation. The degree of sulfatation for the synthetized glycopolymers were all over 75%, for the synthetized glucopolymers in
The approximate mean molecular weight of the sulfated glycopolymer is below 500 000 g/mol, such as below 300 000 g/mol. In one embodiment, the approximate mean molecular weight of the sulfated glycopolymer is from 3500 to 275000 g/mol, such as from 7000 to 180000 g/mol.
The synthesized fucodian-mimetic sulfated glycopolymers may have integrated chemical functionalities, such as linkers for attaching the synthesized fucodian-mimetic glycopolymers to surfaces or matrices. Such features may be biotinylation for streptavidin affinity binding, PEGylation, to increase solubility, prolong stability, and reduce immunogenicity or chemical cross-linking etc.
Dextran is a complex branched glucan (polysaccharide derived from the condensation of glucose). IUPAC defines dextrans as “Branched poly-α-d-glucosides of microbial origin having glycosidic bonds predominantly C-1→C-6”. Dextran chains are of varying lengths (from 3 to 2000 kilodaltons). Dextran sulfate is dextran containing approximately 13% to 21%, such as 17%, sulphur (17% is equivalent to approximately 2.3 sulfate groups per glucosyl residue). Dextran sulfate is a heparin analogue.
In one embodiment, the polysulfated polysaccharide is a dextran sulfate.
In one embodiment, the approximate mean molecular weight of the dextran sulfate is 250000 g/mol or higher, such as 250000 to 2000000 g/mol, such as 500000 g/mol or higher, such as 500000 to 2000000 g/mol.
In one embodiment, the approximate sulfur content of the dextran sulfate is from 13 to 21%, such as 15 to 19%, such as 17%.
In one embodiment, the approximate sulfur content of the dextran sulfate is approximately 1.8 to 2.8, such as 2.0 to 2.6, such as 2.3 sulfate groups per glucosyl residue.
The platelet endothelial aggregation receptor 1 (PEAR1) agonist in the invention may be in any suitable form including powders, gels, solution, or any combination of these. To facilitate use of the platelet endothelial aggregation receptor 1 (PEAR1) agonist it may also be in the form or a topical haemostatic, a sealant, or an adhesive, to be used during surgery. In the specific example of acute aortic dissection (AD), such powders, gels, solutions or topical haemostatic, sealant, or adhesive may be used at sites of the joining between the aorta and the graft, as well as at the stitching.
Topical haemostatics, sealants, and adhesives are used as normal in the art, that is until the desired effect is reached. However, in the invention, these can be used on patients under antiplatelet medication.
In one embodiment, is provided a topical haemostatic, a sealant, or an adhesive, wherein the topical haemostatic, sealant, or adhesive comprises a platelet endothelial aggregation receptor 1 (PEAR1) agonist according to any one of claims 1 to 18, for use in the treatment of bleeding in a patient, wherein the patient is under antiplatelet medication.
In one embodiment, the topical haemostatic, the sealant, or the adhesive, for use in the treatment of bleeding in a patient, wherein the use is local use.
In one embodiment, the topical haemostatic, the sealant, or the adhesive, for use in the treatment of bleeding in a patient, wherein the use is in the treatment of a bleeding in a patient during surgery.
In one embiodiment, a medical patch comprising a topical haemostatic, a sealant, or an adhesive, for use in the treatment of bleeding in a patient, wherein the patient is under antiplatelet medication.
In one embodiment, a topical haemostatic, a sealant, or an adhesive, to be used locally during surgery, wherein the topical haemostatic, a sealant, or an adhesive comprises a platelet endothelial aggregation receptor 1 (PEAR1) agonist for treating bleeding in a patient under antiplatelet medication. In one embodiment, the platelet endothelial aggregation receptor 1 (PEAR1) agonist is a sulfated α-L-fucoside-pendant glycopolymer, natural fucoidan, or dextran sulfate for treating bleeding in a patient under antiplatelet medication. In one further embodiment, a medical patch comprises the topical haemostatic, sealant, or adhesive.
Materials and Methods
Chemicals
Collagen was obtained from Chrono-Log (Chrono-log Corporation, Havertown, Pa., USA), the PAR1-activating peptide SFLLRN was custom-synthesized by JPT Peptide Technologies (Berlin, Germany). Fucoidan from Fucus vesiculosus (purity 95%) was purchased from Sigma Aldrich Sweden AB (Stockholm, Sweden). The synthetic sulfated α-L-fucoside-pendant glycopolymer with average monomeric units of 13, 34 or 329 fucose molecules was a kind gift of Prof. Peter Konradsson, Department of Physics, Chemistry and Biology (IFM)/Chemistry (KEMI), Linkoping University, Linkoping, Sweden, and was generated as described before [2, 3]. The ADP/P2Y12 receptor antagonist AR-C 66096 as well as the TXA2/TPα receptor antagonist ICI 192,605 were purchased from Tocris, UK.
Preparation of Platelet-Rich Plasma (PRP) and Platelet-Poor Plasma (PPP)
With approval from the regional ethical board Örebro-Uppsala county (Dnr 2015/543) blood was obtained from healthy volunteers, who denied to have taken any medication two weeks prior to donation, by venipuncture and drawn into 10 ml syringes preloaded by the supplier with trisodium citrate solution (Sarstedt, Nümbrecht, Germany, Order number: 02.1067.001) The blood was and centrifuged at 220 g for 20 min. The PRP thus obtained was transferred into fresh 15 ml polypropylene tubes, and the remaining blood was centrifuged at 2200 g for 20 min to separate the PPP from remaining blood cells.
Platelet Aggregation in PRP
Measurements were performed at 37° C. using Chrono-Log lumi-aggregometer (Model 700, Chrono-log Corporation, Havertown, Pa., USA) with stirring at 900 rev./min using a final volume of 0.3 ml platelet suspension. Aggregation is expressed as percentage light transmission compared with PPP alone (=100%).
Platelets were preincubated with vehicle or AR-C66096 as indicated for 5 min followed by the stimulation with 1 μg/ml collagen, 10 μM SFLLRN, 30 μg/ml C34, 10 μg/ml fucoidan, or 30 μg/ml dextran sulfate (DxS) (
Platelets were preincubated with vehicle or ICI 192,506 as indicated for 5 min followed by the stimulation with 1 μg/ml collagen, 10 μM SFLLRN, 30 μg/ml C34, 10 μg/ml fucoidan, or 30 μg/ml dextran sulfate (DxS) (
Platelets were preincubated with vehicle or AR-C66096 and ICI 192,506 as indicated for 5 min followed by the stimulation with 1 μg/ml collagen, 10 μM SFLLRN, 30 μg/ml C34, 10 μg/ml fucoidan, or 30 μg/ml dextran sulfate (DxS) (
Glycopolymer Synthesis
2-methacrylamidoethyl 2,3,4-tri-O-acetyl-α-L-fucopyranoside was dissolved in 1,4-dioxane (1.20 M) and CPBDT was added. The solution was transferred to a tube containing AIBN and a stir bar and flushed with N2 (g) for 30 min The tube was sealed and lowered into an oil bath pre-heated to 90° C. The solution was stirred at 90° C. for 24 h, diluted with 1,4-dioxane and freeze-dried. The crude solid compound was purified by suspension in diethyl ether, centrifuged, and isolated. This process was repeated a total of three times to yield the per-acetylated dithiobenzoate-terminated glycopolymer as a pink powder. The per-acetylated dithiobenzoate-terminated glycopolymer was then dissolved in 1,4-dioxane (0.45 mL per mmole monomer unit). The solution was transferred to a tube with a magnetic stirrer and AIBN (6.6 eq. per glycopolymer chain). The tube was flushed with N2 (g) for 30 min. The tube was sealed and submerged in an oil bath pre-heated to 90° C. The solution was stirred overnight, diluted with 1,4-dioxane and lyophilized. The crude per-acetylated glycopolymer was suspended in diethyl ether, centrifuged, and isolated to yield the per-acetylated glycopolymer as a white powder. This process was performed a total of three times. The per-acetylated glycopolymer was suspended in methanol (0.08 mL per mmole monomer units), sodium methoxide (1 eq. per mole monomer units) added, and stirred overnight at RT. The solution was neutralized by Dowex Marathon C (H+), filtered and the solvent evaporated. The crude glycopolymer was dissolved in deionized water and purified by dialysis against deionized water for 48 h with frequent changes of deionized water. Lyophilization gave the glycopolymer as a white fluffy powder, glucopolymer.
To furnish the fucoidan-mimetic glycopolymers, the polymer (2) was sulfated by the following reaction: The glycopolymer was dissolved in DMF (0.01 mL per mmole monomer units) by vigorous stirring. Sulfur trioxide-pyridine complex (5 eq. per free hydroxyl group) was added and the solution stirred for 44 h at RT. The solution was decanted and the precipitated crude O-sulfated glycopolymer was dissolved in 0.9 M NaCl (aq) (90 mmole NaCl per mmole monomer units). The solution was stirred for 46 h at RT and dialyzed against 0.1 M NaCl for 19 h and then against deionized water for 24 h with frequent changes of deionized water. The resulting solution was lyophilized to give the O-sulfated glycopolymer as a white fluffy powder.
The synthesis resulted in a sulfated polymer with a degree of sulfatation of 87-89%. Calculating the effect of adding sulfates, the approximate mean molecular weight for C13 would be around 7260 g/mol, C34 would be around 16470 g/mol and C239 would be around 178300 g/mol.
Results
In the results shown in
As shown in
Platelet aggregation induced by collagen and SFLLRN were markedly reduced, from 90±3% to 21±4% and 91±3% to 15±6%, respectively. The responses induced by the synthetic sulfated α-L-fucoside-pendant glycopolymer (C34: 91±3% down to 45±7%, P≤0.001), natural fucoidan from F. vesicolosus (FV) (96±2% down to 44±6%, P≤0.001), as well as of dextran sulfate (DxS) (88±6% down to 39±7%, P≤0.001) were affected to a lesser extent but nonetheless significantly by ICI 192,605. Nonetheless, the extents of these latter responses were under our settings, with the exception of the experiments using dextran sulfate, significantly higher than those provoked be collagen or SFLLRN in the presence of ICI 165,605.
We finally evaluated human platelet aggregation in plasma evoked by collagen, the PAR-1 activating peptide SFLLRN, the synthetic sulfated α-L-fucoside-pendant glycopolymer with average monomeric units of 34 fucose molecules (C34), natural fucoidan from F. vesicolosus (FV), as well as of dextran sulfate (DxS) under conditions were both P2Y12 receptors and TPα receptors were blocked with AR-C66096 and ICI 192,605, respectively. Platelets were preincubated with vehicle or AR-C66096 and ICI 192,506 as indicated for 5 min followed by the stimulation with 1 μg/ml collagen, 10 μM SFLLRN, 30 μg/ml C34, 10 μg/ml fucoidan from F. vesiculosus (FV), or 30 μg/ml dextran sulfate (DxS). The results are summarized in
Although the present invention has been described above with reference to (a) specific embodiment(s), it is not intended to be limited to the specific form set forth herein. Rather, the invention is limited only by the accompanying claims and, other embodiments than the specific above are equally possible within the scope of these appended claims, e.g. different than those described above.
In the claims, the term “comprises/comprising” does not exclude the presence of other elements or steps. Furthermore, although individually listed, a plurality of means, elements or method steps may be implemented by e.g. a single unit or processor. Additionally, although individual features may be included in different claims, these may possibly advantageously be combined, and the inclusion in different claims does not imply that a combination of features is not feasible and/or advantageous. In addition, singular references do not exclude a plurality. The terms “a”, “an”, “first”, “second” etc do not preclude a plurality. Reference signs in the claims are provided merely as a clarifying example and shall not be construed as limiting the scope of the claims in any way.
| Number | Date | Country | Kind |
|---|---|---|---|
| 1950885-2 | Jul 2019 | SE | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2020/069417 | 7/9/2020 | WO |
