Claims
- 1. A trisubstituted triazine library.
- 2. A method for preparing a trisubstituted triazine library comprising:
a. making a first building block by adding a first amine by reductive amination of triazine; b. making a second building block by adding a second amine to cyanuric chloride; c. combining said first building block with said second building block by aminating the first building block onto one of the chloride positions of the second building block; d. reacting a third building block with the combined first and second building blocks to produce a trisubstituted triazine.
- 3. The process according to claim 2 wherein the first amine is selected from the group consisting of amines substituted with at least one of a C1-14 alcohol or amino group, a C1-14 alkyl group, phenyl substituted with at least one of F, Cl, methoxy, ethoxy, trifluoromethyl, and C1-6 alkyl; and benzyl substituted with at least one of F, Cl, methoxy, ethoxy, trifluoromethyl, and C1-6 alkyl; and the second amine is substituted with at least one of a C1-14 amino group , a C1-14 alkyl group, phenyl substituted with at least one of F, Cl, methoxy, ethoxy, trifluoromethyl, and C1-6 alkyl; and benzyl substituted with at least one of F, Cl, methoxy, ethoxy, trifluoromethyl, and C1-6 alkyl.
- 4. The process according to claim 2 wherein the first building block is selected from compounds of the formula:
wherein R1 is selected from the group consisting of 72737475
- 5. A process for synthesizing a triazine library with linker comprising reacting a trisubstituted triazine according to claim 1 with a linker.
- 6. The process according to claim 5 wherein the linker is 2-[2-amino-ethoxy-ethyoxyethyl]carbamic tert-butyl ester.
- 7. Triazine-linker compounds comprising a trisubstituted triazine bonded to a linker.
- 8. The compounds according to claim 7 selected from compounds of the following formula:
- 9. Affinity matrix beads comprising a triazine linker compound according to claim 7 loaded onto activated beads.
- 10. The affinity matrix beads according to claim 9 wherein the beads are agarose.
- 11. A high density small molecule chip comprising a surface onto which are linked triazine linker compounds according to claim 7.
- 12. The high density small molecule chip according to claim 11 wherein the surface is a glass slide.
- 13. The high density small molecule chip according to claim 11 wherein the amino end of the linker is connected to an activated functional group on the surface.
- 14. The high density small molecule chip according to claim 13 wherein the activated functional group is selected from the group consisting of isocyanate, isothiocyanate, and acyl imidazole.
- 15. A method for determining the binding affinity of proteins to a plurality of molecules comprising incubating a high density small molecule chip according to claim 11 with a plurality of labeled proteins, and analyzing the labels to determine which molecule have affinity for which proteins.
- 16. The method according to claim 15 wherein the label is a florescent label.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] The present application claims priority from non-provisional application Serial No. 60/339,294, filed Dec. 12, 2001, the entire contents of which are hereby incorporated by reference.
Provisional Applications (1)
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Number |
Date |
Country |
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60339294 |
Dec 2001 |
US |