Patent Application
20160237081
The disclosure generally relates to compounds of formula I, including their salts, as well as compositions and methods of using the compounds. The compounds are ligands, agonists, or partial agonists for the mGluR2 PAM receptor and may be useful for the treatment of various disorders of the central nervous system.
Glutamate is the major excitatory neurotransmitter in the mammalian brain, playing an important physiological role in a wide variety of processes. Glutamatergic neurotransmission is predominantly mediated through activation of cell surface receptors including ligand-gated ion channels (ionotropic receptors) and metabotropic glutamate G protein coupled receptors (mGluRs). The metabotropic glutamate receptor family is comprised of 8 family members that are part of the family 3 GPCR superfamily. These receptors are further subdivided into Group I (mGluR 1, 5), Group II (mGluR 2, 3) and Group III (mGluR 4, 6, 7, 8) based upon sequence homology, receptor signaling, and pharmacology.
At the cellular level, mGluR2 plays a key role as an autoreceptor in glutamate terminals, though it is generally thought to be localized at the periphery of the synapse, away from the active zone. Activation of the mGluR2 receptor by glutamate or other orthosteric ligands results in a reduction of adenylate cyclase via a Gai protein and a subsequent reduction of glutamate release from the presynaptic terminal. mGluR2 receptors are localized to regions of the brain involved with psychiatric disorders, including the prefrontal cortex, striatum, hippocampus, and amygdala. Excessive glutamate release has been hypothesized to contribute to the underlying pathophysiology in both anxiety and schizophrenia; therefore, activators of mGluR2 receptors may offer therapeutic benefits in these disorders. This biological phenomenon was demonstrated pre-clinically in a study by Moghaddam and Adams (1998) in which they treated rats with phencyclidine (PCP), an NMDA receptor blocker, and detected increased glutamate release in the mPFC and striatum of these animals as well as hyper-locomotion and working memory deficits. The mGluR2/3 agonist, LY-354740, lowered brain glutamate levels and reversed the behavioral effects of PCP. Many more studies have demonstrated efficacy in a variety of pre-clinical models of psychosis and anxiety with mGluR2/3 agonists. Such pre-clinical work led to the development of mGluR2/3 agonists for both anxiety and schizophrenia. Eli Lilly reported therapeutic effects of LY-544344 for anxiety in GAD patients (Dunayevich et al., 2008) and with LY-2140023 for relief of positive and negative symptoms in schizophrenia (Patil et al., 2007).
To date, most of the available pharmacological tools targeting the mGluR2 receptor have been structural analogues of glutamate and act as orthosteric agonists. While demonstrating proof of principle for use in psychiatric disease, agonists have poor pharmacokinetic profiles and poor brain penetration. Furthermore, several pre-clinical studies have demonstrated tolerance to mGluR2/3 agonists upon repeated dosing in rodents (Cartmell et al., 2000; Galici et al., 2005; Jones et al., 2005). Unlike orthosteric agonists, positive allosteric modulators (PAMs) only activate the receptor when glutamate or another orthosteric agonist is present. Therefore, PAMs are thought to retain spatial and temporal activity of glutamate transmission in the brain and would not continuously stimulate the mGluR2 receptor, potentially avoiding tolerance or unwanted side effects of the agonists. Furthermore, since PAMs bind to an allosteric site on the receptor, they can be designed to be selective for the mGluR2 receptor. Pre-clinical studies and early development of mGluR2 PAMs suggest that they will be effective therapies for positive and negative symptoms and co-morbidy anxiety in schizophrenia.
Based on the expression pattern and functional role of mGluR2, this receptor has emerged as an important target for drug discovery in a number of therapeutic indications. In clinical trials, activating mGluR2 was shown to be efficacious in treating anxiety disorders. In addition, activating mGluR2 has been shown to be efficacious in various animal models of schizophrenia, epilepsy, addiction/drug dependence, Parkinson's disease, pain, sleep disorders, and Huntington's disease. See the following publications: Positive allosteric modulators of the metabotropic glutamate receptor 2 for the treatment of schizophrenia. Mark E Fraley; Expert Opin. Ther. Patents (2009) 19(8); Biphenyl-indanone A, a positive allosteric modulator of the metabotropic glutamate receptor subtype 2, has antipsychotic- and anxiolytic-like effects in mice. Galici Ruggero; et al. The Journal of Pharmacology and Experimental Therapeutics (2006), 318(1), 173-85; Potential psychiatric applications of metabotropic glutamate receptor agonists and antagonists. Krystal, John; et al. CNS Drugs (2010), 24(8), 669-693; Postsynaptic and presynaptic group II metabotropic glutamate receptor activation reduces neuronal excitability; in rat midline paraventricular thalamic nucleus. Hermes M L H J; et al.; The Journal of Pharmacology and Experimental Therapeutics (2011), 336(3), 840-9; Scaffold hopping from pyridones to imidazo[1,2-a]pyridines. New positive allosteric modulators of metabotropic glutamate 2 receptor. Gary Tresadern, et al.; Bioorganic & Medicinal Chemistry Letters 20 (2010) 175-179; 3-Benzyl-1,3-oxazolidin-2-ones as mGluR2 positive allosteric modulators: Hit-to lead and lead optimization. Allen J. Duplantier, et al.; Bioorganic & Medicinal Chemistry Letters 19 (2009) 2524-2529. Use of mGluR2 PAMs for the treatment of cocaine dependence: Design and synthesis of an orally active metabotropic glutamate receptor subtype-2 (mGluR2) positive allosteric modulator (PAM) that decreases cocaine self-administration in rats. Dhanya, Raveendra-Panickar; et al.; Journal of Medicinal Chemistry (2011), 54(1), 342-353; The mGluR2 Positive Allosteric Modulator BINA Decreases Cocaine Self-Administration and Cue-Induced Cocaine-Seeking and Counteracts Cocaine-Induced Enhancement of Brain Reward Function in Rats. Jin, Xinchun; et al.; Neuropsychopharmacology (2010), 35(10), 2021-2036.
The invention provides technical advantages, for example, the compounds are novel and are ligands for the mGluR2 receptor and may be useful for the treatment of various disorders of the central nervous system. Additionally, the compounds provide advantages for pharmaceutical uses, for example, with regard to one or more of their mechanism of action, binding, inhibition efficacy, target selectivity, solubility, safety profiles, or bioavailability.
The invention encompasses compounds of Formula I, including pharmaceutically acceptable salts, pharmaceutical compositions, and their use in treating anxiety, schizophrenia, epilepsy, addiction/drug dependence, Parkinson's disease, pain, sleep disorders, or Huntington's disease, or other neurological or psychiatric disorders associated with glutamate dysfunction.
One aspect of the invention is a compound of formula I
wherein:
R1 is selected from the group consisting of hydrogen, cyano, halo, alkyl, haloalkyl, (cycloalkyl)alkyl, cycloalkyl, alkoxy, and haloalkoxy;
R2 is selected from the group consisting of alkyl, haloalkyl, (cycloalkyl)alkyl, (halocycloalkyl)alkyl, (alkoxy)alkyl, (haloalkoxy)alkyl, cycloalkyl, halocycloalkyl, (alkyl)cycloalkyl, and (dialkyl)cycloalkyl;
R3 is Ar1 or OAr1;
Ar1 is phenyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, or triazinyl, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, cycloalkyl, alkoxy, cycloalkylalkoxy, and haloalkoxy;
Y is a bond or C3-6 cycloalkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy; and
Z is a bond or C1-3 alkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy;
provided that where Y and Z are bonds, R3 is Ar1;
or a pharmaceutically acceptable salt thereof.
Another aspect of the invention is a compound of formula I where:
R1 is selected from the group consisting of hydrogen, cyano, halo, alkyl, haloalkyl, (cycloalkyl)alkyl, cycloalkyl, alkoxy, and haloalkoxy;
R2 is selected from the group consisting of alkyl, haloalkyl, (cycloalkyl)alkyl, (halocycloalkyl)alkyl, (alkoxy)alkyl, (haloalkoxy)alkyl, cycloalkyl, and halocycloalky;
R3 is Ar1 or OAr1;
Ar1 is phenyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, or triazinyl, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, cycloalkyl, alkoxy, cycloalkoxy, and haloalkoxy;
Y is a bond or C3-6 cycloalkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy; and
Z is a bond or C1-3 alkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy;
provided that where Y and Z are bonds, R3 is Ar1;
or a pharmaceutically acceptable salt thereof.
Another aspect of the invention is a compound of formula I where R1 is haloalkyl; R2 is (cycloalkyl)alkyl; R3 is Ar1 or OAr1; Ar1 is phenyl, pyridinyl, or pyrimidinyl, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, cycloalkyl, alkoxy, cycloalkoxy, and haloalkoxy; Y is a bond or C3-6 cycloalkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy; and Z is a bond or C1-3 alkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy; or a pharmaceutically acceptable salt thereof.
Another aspect of the invention is a compound of formula I where R1 is haloalkyl.
Another aspect of the invention is a compound of formula I where R2 (cycloalkyl)alkyl.
Another aspect of the invention is a compound of formula I where R3 is Ar1.
Another aspect of the invention is a compound of formula I where Ar1 is phenyl, pyridinyl, or pyrimidinyl, and is substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, cycloalkyl, alkoxy, cycloalkoxy, and haloalkoxy.
Another aspect of the invention is a compound of formula I where Ar1 is phenyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, cycloalkyl, alkoxy, cycloalkoxy, and haloalkoxy.
Another aspect of the invention is a compound of formula I where Y is C3-6 cycloalkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy.
Another aspect of the invention is a compound of formula I where Y is a bond and Z is C1-3 alkyl substituted with 0-3 substituents selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy.
Another aspect of the invention is a compound of formula I where —Y—Z— is 1,4-cyclohexanddiyl, 1,3-cyclobutanddiyl, or (cyclopropyl)methyl, and is substituted with 1 substituent selected from cyano, halo, alkyl, haloalkyl, hydroxy, alkoxy, and haloalkoxy.
For a compound of formula I, the scope of any instance of available substituent, including R1, R2, R3, Ar1, X1, X2, X3, Y, and Z, can be used independently with the scope of any other instance of a variable substituent. As such, the invention includes combinations of the different aspects.
Unless specified otherwise, these terms have the following meanings “Halo” includes fluoro, chloro, bromo, and iodo. “Alkyl” means a straight or branched alkyl group composed of 1 to 6 carbons. “Alkenyl” means a straight or branched alkyl group composed of 2 to 6 carbons with at least one double bond. “Alkynyl” means a straight or branched alkyl group composed of 2 to 6 carbons with at least one triple bond. “Cycloalkyl” means a monocyclic ring system composed of 3 to 7 carbons. For clarity, a cycloalkyl moiety may be substituted at different positions, for example vicinal-disubstituted or gem-disubstituted. “Haloalkyl” and “haloalkoxy” include all halogenated isomers from monohalo to perhalo. Terms with a hydrocarbon moiety (e.g. alkoxy) include straight and branched isomers for the hydrocarbon portion. Parenthetic and multiparenthetic terms are intended to clarify bonding relationships to those skilled in the art. For example, a term such as ((R)alkyl) means an alkyl substituent further substituted with the substituent R.
The invention includes all pharmaceutically acceptable salt forms of the compounds. Pharmaceutically acceptable salts are those in which the counter ions do not contribute significantly to the physiological activity or toxicity of the compounds and as such function as pharmacological equivalents. These salts can be made according to common organic techniques employing commercially available reagents. Some anionic salt forms include acetate, acistrate, besylate, bromide, chloride, citrate, fumarate, glucouronate, hydrobromide, hydrochloride, hydroiodide, iodide, lactate, maleate, mesylate, nitrate, pamoate, phosphate, succinate, sulfate, tartrate, tosylate, and xinofoate. Some cationic salt forms include ammonium, aluminum, benzathine, bismuth, calcium, choline, diethylamine, diethanolamine, lithium, magnesium, meglumine, 4-phenylcyclohexylamine, piperazine, potassium, sodium, tromethamine, and zinc.
The invention includes all stereoisomeric forms of the compounds, both mixtures and separated isomers. Mixtures of stereoisomers can be separated into individual isomers by methods known in the art.
The invention is intended to include all isotopes of atoms occurring in the present compounds. Isotopes include those atoms having the same atomic number but different mass numbers. By way of general example and without limitation, isotopes of hydrogen include deuterium and tritium. Isotopes of carbon include 13C and 14C. Isotopically-labeled compounds of the invention can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described herein, using an appropriate isotopically-labeled reagent in place of the non-labeled reagent otherwise employed. Such compounds may have a variety of potential uses, for example as standards and reagents in determining biological activity. In the case of stable isotopes, such compounds may have the potential to favorably modify biological, pharmacological, or pharmacokinetic properties.
Compounds of Formula I may be made by methods known in the art including those described below and including variations within the skill of the art. Some reagents and intermediates are known in the art. Other reagents and intermediates can be made by methods known in the art using readily available materials. The formula and variable designations used to describe the synthesis of the compounds are intended only to illustrate how to make the compounds and are not to be confused with variables used in the claims or in other sections of the specification. The following methods are for illustrative purposes and are not intended to limit the scope of the invention. The schemes encompass reasonable variations known in the art.
Triazolopyridine ethers of formula (I) can be prepared in a number of ways known to those skilled in the art. Schemes 1-4 illustrate different methods that can be useful for the preparation of the titled compounds. In scheme 1, variously substituted alcohols R3OH can be added to 2,4-dichloropyridines 2 to afford 2-chloropyridyl ethers 3. The pyridyl ethers can be readily converted to the corresponding 2-hydrazinylpyridine ethers 3 upon treatment with hydrazine. A wide variety of acylhydrazides 5 can be prepared from the coupling of 2-hydrozinylpyridine ethers 3 with acylchlorides or carboxylic acids under standard conditions known to one skilled in the art. Cyclization of the acylhydrazide 4 to afford triazolopyridine ethers of formula (I) can be accomplished using mild dehydrating reagents including, but not limited to, 1-methoxy-N-triethylammoniosulfonyl-methanimidate, otherwise known as the Burgess reagent. Alcohols R3OH, 2,4-dichloropyridines 2, carboxylic acids R1CO2H, and acyl chlorides R1C(O)Cl used to prepared compounds of formula (I) may be commercially available or readily prepared by employing standard methods known to those skilled in the art.
A second approach to the formation of triazolopyridine compounds of formula (I), is depicted in scheme 2. Benzylic ethers of formula 4a where Ar is phenyl or substituted phenyl, can be converted in a single reaction using phosphoryl chloride into the substituted 7-chlorotriazolopyridines 5. Coupling of the 7-chlorotriazolopyridines 5 with various alcohols R3OH under modified Buchwald-Hartwig type conditions can afford the titled compounds of formula (I). The ether coupling may be conducted using a mixture of reagents including, but not limited to, cesium carbonate, (S)—(R)-JOSIPHOS, and allylpalladium chloride dimer.
Another approach to the formation of triazolopyridine compounds of formula (I), is outlined in scheme 3. In this route, benzylic triazolopyridine ethers of formula 6, which can be obtained via scheme 1, are hydrogenolytically cleaved to provide the 7-hydroxyltriazolopyridines 7. Alkylation of 7-hydroxyltriazolopyridines 7 with electrophiles R3X under a variety of standard conditions known to one skilled in the art can provide compounds of formula (Ia). Electrophiles that are useful for this reaction include, but are not limited to, substituted benzylic halides.
Chlorotriazolopyridine ethers of formula (Ib) can be prepared according to the route illustrated in scheme 4. The ethers (Ib) can result from a metal mediated coupling of a triazolopyridine iodide 10 with an alcohol R3OH. The requisite iodide can be prepared in two steps from the known iodopyridine 8 using standard conditions known one skilled in the art.
Clonally expressed human mGluR2 in HEK293 background was glutamate starved overnight in media without glutamine (GIBCO MEM 12360), containing 10% FBS, 1% penicillin streptomycin.
cAMP Measurement.
On the day of the assay, media was removed, cells washed with PBS, cells harvested and pelleted by centrifugation. Cell pellets were resuspended in stimulation buffer without forskolin and counted. A solution of 1.25×106 cells/mL was prepared and dispensed to plates using a Combi liquid handler (Thermo). A standard curve was created using a top concentration of cAMP of 1 μM in the stimulation buffer, 1:3 dilutions over 14 points in the absence of cells. Plates were read by time-resolved fluorescence at 665 nM and 618 nM and a ratio was calculated via in house software, (665 nM/618 nM)*104. In house software converted the fluorescence value ratios to units of cAMP concentration, which is then used to calculate % inhibition for each test compound.
MGluR2 cAMP Assay Materials and Methods.
Compounds were added to white, standard volume 384 non-binding surface plates (Corning 3574). Cells were resuspended in stimulation buffer consisting of Hanks Balanced Salt Solution (14175-095) pH 7.0, 20 mM HEPES, 2.0 mM CaCl2, 5.0 mM MgCl2, and 1 mM IBMX (Sigma 15879), 1 μM forskolin, and 1 μM LY-341495 for 30 min. Buffer without forskolin was used as a negative control. Solutions of D2 and cryptate detection reagents from the CISBIO dynamic cAMP kit (62AM4PEJ) were diluted 1:40 in lysis buffer. Lysis buffer consisted of 50 mM Phosphate Buffer pH 7.0, 800 mM Potassium Fluoride, 0.2% BSA, and 1.0% Triton. Assay reaction was terminated by addition of detection reagents in lysis buffer. One hour later, plates were read on a PE Viewlux. Data was extracted, and concentration response curves generated by fitting the data to a standard 4 parameter logistic equation from which EC50 values were determined. See table 1.
Compounds of formula I modulate to mGluR2 and can be useful in treating neurological or psychiatric disorders. Therefore, another aspect of the invention is a composition comprising a compound of formula I or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
Another aspect of the invention is a method for the treatment of anxiety, schizophrenia, epilepsy, addiction/drug dependence, Parkinson's disease, pain, sleep disorders, or Huntington's disease, or other neurological or psychiatric disorders associated with glutamate dysfunction, which comprises administering to a patient a therapeutically affective amount of a compound of formula I.
Another aspect of the invention is a method for the treatment of anxiety or schizophrenia which comprises administering to a patient a therapeutically affective amount of a compound of formula I.
Another aspect of the invention is a method for the treatment of anxiety which comprises administering to a patient a therapeutically affective amount of a compound of formula I.
Another aspect of the invention is a method for the treatment of schizophrenia which comprises administering to a patient a therapeutically affective amount of a compound of formula I.
Another aspect of the invention is the use of a compound of formula I in the manufacture of a medicament for the treatment of neurological or psychiatric disorders.
Another aspect of the invention is the use of a compound of formula I in the manufacture of a medicament for the treatment of anxiety, schizophrenia, epilepsy, addiction/drug dependence, Parkinson's disease, pain, sleep disorders, or Huntington's disease, or other neurological or psychiatric disorders associated with glutamate dysfunction.
Another aspect of the invention is the use of a compound of formula I in the manufacture of a medicament for the treatment of anxiety.
Another aspect of the invention is the use of a compound of formula I in the manufacture of a medicament for the treatment of schizophrenia.
“Patient” means a person suitable for therapy as understood by practitioners in the field of affective disorders and neurodegenerative disorders.
“Treatment,” “therapy,” and related terms are used as understood by practitioners in the field of neurological and psychiatric disorders.
The compounds of this invention are generally given as pharmaceutical compositions comprised of a therapeutically effective amount of a compound or its pharmaceutically acceptable salt and a pharmaceutically acceptable carrier and may contain conventional excipients. Pharmaceutically acceptable carriers are those conventionally known carriers having acceptable safety profiles. Compositions encompass all common solid and liquid forms including for example capsules, tablets, losenges, and powders as well as liquid suspensions, syrups, elixers, and solutions. Compositions are made using common formulation techniques, and conventional excipients (such as binding and wetting agents) and vehicles (such as water and alcohols) are generally used for compositions. See, for example, Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, Pa., 17th edition, 1985.
Solid compositions are normally formulated in dosage units and compositions providing from about 1 to 1000 mg of the active ingredient per dose are preferred. Some examples of dosages are 1 mg, 10 mg, 100 mg, 250 mg, 500 mg, and 1000 mg. Generally, other agents will be present in a unit range similar to agents of that class used clinically. Typically, this is 0.25-1000 mg/unit.
Liquid compositions are usually in dosage unit ranges. Generally, the liquid composition will be in a unit dosage range of 1-100 mg/mL. Some examples of dosages are 1 mg/mL, 10 mg/mL, 25 mg/mL, 50 mg/mL, and 100 mg/mL. Generally, other agents will be present in a unit range similar to agents of that class used clinically. Typically, this is 1-100 mg/mL.
The invention encompasses all conventional modes of administration; oral and parenteral methods are preferred. Generally, the dosing regimen will be similar to other agents used clinically. Typically, the daily dose will be 1-100 mg/kg body weight daily. Generally, more compound is required orally and less parenterally. The specific dosing regime, however, will be determined by a physician using sound medical judgement.
The following experimental procedures describe the synthesis of some Formula I compounds. Standard chemistry conventions are used in the text unless otherwise noted. The experimental encompass reasonable variations known in the art. The following HPLC conditions may be used where indicated.
Abbreviations used in the schemes generally follow conventions used in the art. Chemical abbreviations used in the specification and examples are defined as follows: “NaHMDS” for sodium bis(trimethylsilyl)amide; “DMF” for N,N-dimethylformamide; “MeOH” for methanol; “NBS” for N-bromosuccinimide; “Ar” for aryl; “TFA” for trifluoroacetic acid; “LAH” for lithium aluminum hydride; “BOC” for t-butoxycarbonyl, “DMSO” for dimethylsulfoxide; “h” for hours; “rt” for room temperature or retention time (context will dictate); “min” for minutes; “EtOAc” for ethyl acetate; “THF” for tetrahydrofuran; “EDTA” for ethylenediaminetetraacetic acid; “Et2O” for diethyl ether; “DMAP” for 4-dimethylaminopyridine; “DCE” for 1,2-dichloroethane; “ACN” for acetonitrile; “DME” for 1,2-dimethoxyethane; “HOBt” for 1-hydroxybenzotriazole hydrate; “DIEA” for diisopropylethylamine, “Nf” for CF3(CF2)3SO2—; and “TMOF” for trimethylorthoformate.
Abbreviations as used herein, are defined as follows: “1×” for once, “2×” for twice, “3×” for thrice, “° C.” for degrees Celsius, “eq” for equivalent or equivalents, “g” for gram or grams, “mg” for milligram or milligrams, “L” for liter or liters, “mL” for milliliter or milliliters, “A” for microliter or microliters, “N” for normal, “M” for molar, “mmol” for millimole or millimoles, “min” for minute or minutes, “h” for hour or hours, “rt” for room temperature, “RT” for retention time, “atm” for atmosphere, “psi” for pounds per square inch, “conc.” for concentrate, “sat” or “sat′d” for saturated, “MW” for molecular weight, “mp” for melting point, “ee” for enantiomeric excess, “MS” or “Mass Spec” for mass spectrometry, “ESI” for electrospray ionization mass spectroscopy, “HR” for high resolution, “HRMS” for high resolution mass spectrometry, “LCMS” for liquid chromatography mass spectrometry, “HPLC” for high pressure liquid chromatography, “RP HPLC” for reverse phase HPLC, “TLC” or “tic” for thin layer chromatography, “NMR” for nuclear magnetic resonance spectroscopy, “1H” for proton, “δ” for delta, “s” for singlet, “d” for doublet, “t” for triplet, “q” for quartet, “m” for multiplet, “br” for broad, “Hz” for hertz, and “α”, “β”, “R”, “S”, “E”, and “Z” are stereochemical designations familiar to one skilled in the art.
Standard conditions typically used for preparative LC-MS are as follows: column=XBridge C18, 19×200 mm, 5-μm particles; mobile phase A=5:95 acetonitrile:water with 10-mM ammonium acetate; mobile phase B=95/5 acetonitrile:water with 10-mM ammonium acetate; gradient=30-70% B over 20 minutes, then a 7-minute hold at 100% B; flow=20 mL/min. Standard conditions typically used for reverse phase preparative HPLC are as follows: column=Sunfire PrepC18 OBD 10 μm, 50×250 mm column; mobile phase A=90% MeOH/10% water with 0.1% TFA; mobile phase B=90% MeOH/10% water with 0.1% TFA; linear gradient 10-100% B over 34 min, then a 20 minute hold at 100% B; flow=50 mL/min.
A solution of (1-phenylcyclohexyl)methanol (commercially available, 500 mg, 2.63 mmol) in DMF (3 mL) was added to a mixture of 60% sodium hydride dispersion in mineral oil (163 mg, 4.07 mmol) and DMF (10 mL) in an ice bath at 0° C. The mixture was allowed to stir at 0° C. for 30 min, then a solution of 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422, 596 mg, 2.76 mmol) in DMF (3 mL) was quickly added. The resulting mixture was stirred at 0° C. for 1 hour, then quenched by the addition of water. The aqueous mixture was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over magnesium sulfate, filtered, and concentrated in vacuo. The residue was purified using silica gel column chromatography (5:1 hexanes/ethyl acetate) to afford 2-chloro-4-((1-phenylcyclohexyl)methoxy)-3-(trifluoromethyl)pyridine (875 mg, 90% yield) as a clear viscous oil. LC-MS (M+H)+ 370.0. 1H NMR (500 MHz, chloroform-d) δ ppm 8.25 (d, J=6.0 Hz, 1H), 7.53-7.42 (m, 2H), 7.42-7.33 (m, 2H), 7.27-7.22 (m, 1H), 6.73 (d, J=6.0 Hz, 1H), 3.91 (s, 2H), 2.30 (d, J=13.3 Hz, 2H), 1.90-1.77 (m, 2H), 1.68-1.57 (m, 3H), 1.48-1.35 (m, 3H).
A mixture of 2-chloro-4-((1-phenylcyclohexyl)methoxy)-3-(trifluoromethyl)pyridine (from step A1, 479 mg, 1.30 mmol), dioxane (10 mL), and hydrazine monohydrate (1.28 mL, 16.8 mmol) was heated together in a sealed vial for 18 h in an oil bath at 100° C. The reaction mixture was cooled to rt and concentrated in vacuo. The residue was partitioned between aqueous sodium bicarbonate and dichloromethane. The aqueous layer was extracted with dichloromethane. The combined organic extracts were washed with brine, dried over magnesium sulfate, filtered, and concentrated in vacuo to afford 2-hydrazinyl-4-((1-phenylcyclohexyl)methoxy)-3-(trifluoromethyl)pyridine (437 mg, 92% yield) as a white solid. LC-MS (M+H)+ 366.1. 1H NMR (500 MHz, DMSO-d6) δ 8.10 (d, J=5.8 Hz, 1H), 7.44 (d, J=7.5 Hz, 2H), 7.33 (t, J=7.7 Hz, 3H), 7.24-7.12 (m, 1H), 6.46 (d, J=6.0 Hz, 1H), 4.25 (s, 2H), 3.96 (s, 2H), 2.19 (d, J=13.7 Hz, 2H), 1.84-1.69 (m, 2H), 1.62-1.41 (m, 3H), 1.39-1.20 (m, 3H).
2-Chloro-4-(2-(4-chlorophenyl)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (810 mg, 82% yield) was prepared from 2-(4-chlorophenyl)-2-methylpropan-1-ol (commercially available) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 363.9. 1H NMR (500 MHz, chloroform-d) δ 8.32 (d, J=5.8 Hz, 1H), 7.39-7.29 (m, 4H), 6.82 (d, J=5.8 Hz, 1H), 4.00 (s, 2H), 1.49 (s, 6H).
4-(2-(4-Chlorophenyl)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (457 mg, quantitative yield) was prepared from 2-chloro-4-(2-(4-chlorophenyl)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (from step B1) following a procedure analogous to step A2. LC-MS (M+H)+ 360.1. 1H NMR (500 MHz, DMSO-d6) δ 8.16 (d, J=5.8 Hz, 1H), 7.52-7.41 (m, 2H), 7.41-7.30 (m, 3H), 6.55 (d, J=6.0 Hz, 1H), 4.26 (s, 2H), 4.11 (s, 2H), 1.37 (s, 6H).
((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methanol (737 mg, 4.43 mmol, 89% yield) was prepared from (1S,2S)-2-(4-fluorophenyl)cyclopropanecarboxylic acid (WO 2012/155199) following a procedure analogous to preparation F2. LC-MS (M−H2O+H)+=149.2.
2-Bromo-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)nicotinonitrile (244 mg, 47% yield) was prepared from ((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methanol (from preparation F) and 2,4-dibromonicotinonitrile (WO 2009/062676) following a procedure analogous to step A1. The crude was purified using silica gel column chromatography (2:1-1:1 hexanes/ethyl acetate). 1H NMR (500 MHz, chloroform-d) δ 8.37 (d, J=6.0 Hz, 1H), 7.16-7.07 (m, 2H), 7.03-6.94 (m, 2H), 6.89 (d, J=6.0 Hz, 1H), 4.21 (qd, J=10.4, 6.8 Hz, 2H), 2.10-2.01 (m, 1H), 1.61-1.53 (m, 1H), 1.13 (ddt, J=24.0, 8.8, 5.5 Hz, 2H).
4-(((1S,2S)-2-(4-Fluorophenyl)cyclopropyl)methoxy)-2-hydrazinylnicotinonitrile (186 mg, 90% yield) was prepared in from 2-bromo-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)nicotinonitrile (from step D1, 240 mg, 0.691 mmol) following a procedure analogous to step A2. LC-MS (M+H)+ 299.2.
To a solution of diisopropylamine (4.24 mL, 29.7 mmol) in THF (100 mL) at −5° C. under nitrogen was added a solution of 2.5 M n-butyllithium in hexanes (11.9 mL, 29.7 mmol) dropwise via syringe. After stirring for 15 min, methyl 2-(4-fluorophenyl)acetate (commercially available, 5.0 g, 29.7 mmol) was added. The mixture was stirred at −5° C. for 30 min and then treated with iodomethane (1.86 mL, 29.7 mmol). The reaction mixture was allowed to gradually warm to rt. After 4 h at rt, the reaction mixture was poured into a solution of aqueous ammonium chloride. The resulting mixture was extracted with diethyl ether. The combined organic extracts were washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo. The crude product was purified using silica gel column chromatography (20:1 hexane/ethyl acetate) to afford methyl 2-(4-fluorophenyl)propanoate (2.32 g, 43% yield) as a clear liquid. 1H NMR (500 MHz, chloroform-d) δ 7.33-7.25 (m, 2H), 7.09-6.98 (m, 2H), 3.73 (q, J=7.2 Hz, 1H), 3.69 (s, 3H), 1.51 (d, J=7.2 Hz, 3H).
Sodium borohydride (2.41 g, 63.7 mmol) was added portionwise to a solution of methyl 2-(4-fluorophenyl)propanoate (from step E1, 2.32 g, 12.7 mmol) in THF (25 ml) at rt. The mixture was allowed to stir at rt for 18 h. The reaction was quenched with water and adjusted to pH 3-4 with 1 M aqueous hydrochloric acid. The resulting mixture was extracted with ethyl acetate. The combined organic layers were washed with brine, dried (magnesium sulfate), filtered and concentrated in vacuo. The crude product was purified using silica gel column chromatography (2:1-1:1 hexane/ethyl acetate) to afford 2-(4-fluorophenyl)propan-1-ol (0.746 g, 38% yield). 1H NMR (400 MHz, chloroform-d) δ 7.25-7.16 (m, 2H), 7.08-6.97 (m, 2H), 3.77-3.62 (m, 2H), 2.95 (sxt, J=6.9 Hz, 1H), 1.32 (dd, J=6.7, 5.6 Hz, 1H), 1.27 (d, J=7.0 Hz, 3H).
A solution of 1.0 M potassium tert-butoxide in tert-butanol (12.3 mL, 12.3 mmol) was added to a mixture of methyl 2-(2-chloro-6-fluorophenyl)acetate (2.5 g, 12.3 mmol), iodomethane (0.772 mL, 12.3 mmol), and THF (50 mL) maintained at 0° C. After complete addition, the cold bath was removed and the mixture was allowed to stir at rt for 4 days. The reaction was quenched with a solution of aqueous ammonium chloride, poured into water, and extracted with ethyl acetate. The combined organics extracts were washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo. The crude product was purified using silica gel column chromatography (50:1 hexane/ethyl acetate) to afford a sample of methyl 2-(2-chloro-6-fluorophenyl)propanoate (318 mg, 11% yield) contaminated with ˜14% of methyl 2-(2-chloro-6-fluorophenyl)acetate. The impure sample of methyl 2-(2-chloro-6-fluorophenyl)propanoate was carried forward without further purification. LC-MS (M+H)+ 217.1.
A solution of methyl 2-(2-chloro-6-fluorophenyl)propanoate (from step F1, 318 mg, 1.47 mmol) (contaminated with approximately 14% methyl 2-(2-chloro-6-fluorophenyl)acetate) in dry THF (10 mL) was carefully added over 2 min to solution of LAH (111 mg, 2.94 mmol) in dry THF (20 mL) at 0° C. The reaction mixture was allowed to warm to rt and was stirred for 2 h. The reaction was quenched with 0.110 mL of water and then 0.110 mL of 15% NaOH/water. After stirring for 10 min, an additional 0.22 mL of water was added. The mixture was allowed to stir for 3 hours and it was filtered through celite. The filtrate was concentrated in vacuo. The residue was dried under high vacuum for 16 h to afford 2-(2-chloro-6-fluorophenyl)propan-1-ol (0.276 g, 99% yield) as a clear viscous oil. HPLC and 1H NMR data indicated that the product was approximately 86% pure. The impure crude product was carried forward without purification. 1H NMR (500 MHz, chloroform-d) δ 7.22-7.18 (m, 1H), 7.14 (td, J=8.1, 5.7 Hz, 1H), 7.00-6.93 (m, 1H), 3.99-3.92 (m, 1H), 3.92-3.85 (m, 1H), 3.73-3.64 (m, 1H), 1.36 (dd, J=7.0, 1.5 Hz, 3H).
2-Chloro-4-(2-(2-chloro-6-fluorophenyl)propoxy)-3-(trifluoromethyl)pyridine (304 mg, 56% yield) was prepared from 2-(2-chloro-6-fluorophenyl)propan-1-ol (from preparation F) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 368.0.
4-(2-(2-Chloro-6-fluorophenyl)propoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (292 mg, 97% yield) was prepared from 2-chloro-4-(2-(2-chloro-6-fluorophenyl)propoxy)-3-(trifluoromethyl)pyridine (from step G1) according to the methods described in step A2. LC-MS (M+H)+ 364.1.
A solution of 1.0 M potassium tert-butoxide in tert-butanol (92 mL, 92 mmol) was added to a mixture of 2-(4-fluorophenyl)acetonitrile (commercially available, 5.0 g, 37.0 mmol), iodomethane (6.36 mL, 102 mmol), and THF (100 mL) maintained at 0° C. After complete addition, the cold bath was removed and the mixture was allowed to stir at rt for 18 h. The reaction was quenched with a saturated aqueous solution of ammonium chloride, poured into water, and extracted with ethyl acetate. The combined organics were washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo. The crude product was purified using silica gel column chromatography (40:1 hexane/ethyl acetate) to afford 2-(4-fluorophenyl)-2-methylpropanenitrile (5.2 g, 84% yield) as a clear liquid. 1H NMR (500 MHz, chloroform-d) δ 7.50-7.41 (m, 2H), 7.14-7.03 (m, 2H), 1.73 (s, 6H).
A mixture of 2-(4-fluorophenyl)-2-methylpropanenitrile (from step H1, 5.2 g, 31.9 mmol) and 15% aqueous sodium hydroxide (80 mL) was heated at reflux for 16 h. The mixture was cooled to rt and extracted with diethyl ether. The aqueous layer was made acidic with 1 N aqueous hydrochloric acid (300 mL) and was then extracted with diethyl ether. The acidic organic extract was washed with brine, dried (magnesium sulfate), filtered and concentrated in vacuo. The residue was dried under high vacuum for several hours to afford 2-(4-fluorophenyl)-2-methylpropanoic acid (1.89 g, 33% yield) as a white solid. 1H NMR (500 MHz, chloroform-d) δ 7.44-7.32 (m, 2H), 7.09-6.97 (m, 2H), 1.60 (s, 6H).
2-(4-Fluorophenyl)-2-methylpropan-1-ol (1.77 g, quantitative yield) was prepared from 2-(4-fluorophenyl)-2-methylpropanoic acid (from step H2) following a procedure analogous to step F2. 1H NMR (500 MHz, chloroform-d) δ 7.42-7.32 (m, 2H), 7.08-6.98 (m, 2H), 3.61 (s, 2H), 1.34 (s, 6H).
A solution of 2-(4-fluorophenoxy)-2-methylpropanoic acid (commercially available, 2.5 g, 12.6 mmol) in dry THF (10 mL) was carefully added over 2 min to solution of LAH (0.718 g, 18.92 mmol) in dry THF (20 mL) at 0° C. The reaction mixture was allowed to warm to rt and was stirred for 4 h. The reaction was quenched with 0.7 mL of water and 0.7 mL of 1M NaOH. After stirring for 10 min, an additional 1.4 mL of water was added. The mixture was allowed to stir for 2 h, and then was filtered through celite. The filtrate was concentrated in vacuo. The residue was purified using silica gel column chromatography (2:1 hexane/ethyl acetate) to afford 2-(4-fluorophenoxy)-2-methylpropan-1-ol (606 mg, 25% yield) as a clear viscous oil. 1H NMR (500 MHz, chloroform-d) δ 7.03-6.88 (m, 4H), 3.59 (s, 2H), 1.25 (s, 6H).
2-Chloro-4-(2-(4-fluorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (515 mg, 87% yield) was prepared from 2-(4-fluorophenoxy)-2-methylpropan-1-ol (from preparation I) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 364.0. 1H NMR (500 MHz, chloroform-d) δ 8.38 (d, J=5.8 Hz, 1H), 7.01-6.92 (m, 4H), 6.91 (d, J=5.3 Hz, 1H), 4.07 (s, 2H), 1.42 (s, 6H).
4-(2-(4-fluorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (482 mg, 95% yield) was prepared from 2-chloro-4-(2-(4-fluorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (from step J1) following a procedure analogous to step A2. LC-MS (M+H)+ 360.1.
A solution of 1.0 M potassium tert-butoxide in tert-butanol (55.5 mL, 55.5 mmol) was added to a mixture of 2-(4-fluorophenyl)acetonitrile (commercially available, 5.0 g, 37.0 mmol), iodomethane (2.31 mL, 37.0 mmol), and THF (100 mL) maintained at 0° C. After complete addition, the cold bath was removed and the mixture was allowed to stir at rt for 18 h. The reaction was quenched with a saturated aqueous solution of ammonium chloride, poured into water, and extracted with ethyl acetate. The combined organics were washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo. The crude product was purified using silica gel column chromatography (40:1 hexane/ethyl acetate) to afford 2-(4-fluorophenyl)propanenitrile (2.9 g, 53% yield) as a clear liquid. 1H NMR (500 MHz, chloroform-d) δ 7.38-7.30 (m, 2H), 7.12-7.05 (m, 2H), 3.90 (q, J=7.2 Hz, 1H), 1.65 (d, J=7.2 Hz, 3H).
A 40% solution of benzyltrimethylammonium hydroxide in methanol (2.80 g, 6.70 mmol) was added to a mixture of 2-(4-fluorophenyl)propanenitrile (from step K1, 1.0 g, 6.70 mmol) and paraformaldehyde (0.805 g, 26.8 mmol) in pyridine (7 mL). The mixture was left to stir at rt for 18 h. The reaction mixture was poured into water, and extracted with ether. The combined organic extracts were washed brine and dried over magnesium sulfate. The mixture was filtered and concentrated in vacuo. The residue was purified using silica gel column chromatography (2:1 ethyl acetate/hexanes) to afford 2-(4-fluorophenyl)-3-hydroxy-2-methylpropanenitrile (1.2 g, 98% yield) as a clear viscous oil. 1H NMR (500 MHz, chloroform-d) δ 7.50-7.43 (m, 2H), 7.17-7.09 (m, 2H), 3.90-3.79 (m, 2H), 2.01 (br. s., 1H), 1.75 (s, 3H).
3-((2-Chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-2-(4-fluorophenyl)-2-methylpropanenitrile (899 mg, 75% yield) was prepared from 2-(4-fluorophenyl)-3-hydroxy-2-methylpropanenitrile (from preparation K, 600 mg, 3.35 mmol) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 359.0.
2-(4-Fluorophenyl)-3-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)-2-methylpropanenitrile (934 mg, quantitative yield) was prepared from 34-(2-chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-2-(4-fluorophenyl)-2-methylpropanenitrile (from step L1, 890 mg, 2.48 mmol) following a procedure analogous to step A2. LC-MS (M+H)+ 355.1.
2-(4-Chlorophenoxy)-2-methylpropan-1-ol (2.3 g, 11.46 mmol, 98% yield) was prepared from 2-(4-chlorophenoxy)-2-methylpropanoic acid (commercially available) following a procedure analogous to preparation I. 1H NMR (500 MHz, chloroform-d) δ 7.27-7.23 (m, 2H), 6.96-6.90 (m, 2H), 3.59 (s, 2H), 1.27 (s, 6H).
2-Chloro-4-(2-(4-chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (657 mg, 87% yield) was prepared from 2-(4-chlorophenoxy)-2-methylpropan-1-ol (from preparation M) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 380.0.
4-(2-(4-Chlorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (647 mg, quantitative yield) was prepared from 2-chloro-4-(2-(4-chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (from step N1) following a procedure analogous to step A2. LC-MS (M+H)+ 376.1.
A mixture of 2,4-difluorophenol (commercially available, 10 g, 77 mmol), ethyl 2-bromo-2-methylpropanoate (15 g, 77 mmol), and potassium carbonate (10.6 g, 77 mmol) in DMF (50 mL) was stirred at rt for 3 days. The reaction mixture was poured into water. The aqueous mixture was extracted with ethyl acetate/hexane (90/10). The combined organics were washed with water and then brine. The organic layer was dried (magnesium sulfate), filtered and concentrated in vacuo. The residue was purified by silica gel column chromatography (5:1 hexane/ethyl acetate) to afford ethyl 2-(2,4-difluorophenoxy)-2-methylpropanoate (7.07 g, 37% yield) as a clear thin liquid. LC-MS (M+H)+ 245.1. 1H NMR (500 MHz, chloroform-d) δ 7.04 (td, J=9.1, 5.6 Hz, 1H), 6.84 (ddd, J=10.8, 8.2, 3.1 Hz, 1H), 6.75 (dddd, J=9.2, 7.7, 3.0, 1.8 Hz, 1H), 4.26 (q, J=7.1 Hz, 2H), 1.56 (s, 7H), 1.31 (t, J=7.1 Hz, 3H).
2-(2,4-Difluorophenoxy)-2-methylpropan-1-ol (2.05 g, 10.14 mmol, 94% yield) was prepared from ethyl 2-(2,4-difluorophenoxy)-2-methylpropanoate (from step 01) following a procedure analogous to step F2. 1H NMR (500 MHz, chloroform-d) δ 7.04 (td, J=9.1, 5.6 Hz, 1H), 6.90-6.84 (m, 1H), 6.79 (dddd, J=9.1, 7.7, 3.1, 1.7 Hz, 1H).
2-Chloro-4-(2-(2,4-difluorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (555 mg, 72% yield) was prepared from 2-(2,4-difluorophenoxy)-2-methylpropan-1-ol (from preparation O) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 382.0. 1H NMR (500 MHz, chloroform-d) δ 8.39 (d, J=5.8 Hz, 1H), 7.03 (td, J=9.0, 5.7 Hz, 1H), 6.94 (d, J=6.0 Hz, 1H), 6.86 (ddd, J=10.5, 8.4, 3.0 Hz, 1H), 6.83-6.75 (m, 1H), 4.14 (s, 2H), 1.50-1.36 (m, 6H).
4-(2-(2,4-Difluorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (536 mg, 99% yield) was prepared from 2-chloro-4-(2-(2,4-difluorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridine (from step P1) following a procedure analogous to step A2. LC-MS (M+H)+ 378.1.
Sodium borohydride (47.1 mg, 1.246 mmol) was added to a stirred solution of 1-(4-fluorophenyl)-4-oxocyclohexanecarbonitrile [(from J. Med. Chem. 1975, 18, 593) 246 mg, 1.13 mmol] in ethanol (15 mL) maintained in a 0° C. ice bath. After 2 h, the reaction was diluted with water and the pH was adjusted to 2 by the addition of 1 N aqueous hydrochloric acid. The mixture was extracted with ethyl acetate. The combined organics were washed with brine, dried (magnesium sulfate), filtered and concentrated in vacuo to afford (1s,4s)-1-(4-fluorophenyl)-4-hydroxycyclohexanecarbonitrile (250 mg, 99% yield). LC-MS (M+H)+ 220.1.
(1s,4s)-4-((2-Chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-1-(4-fluorophenyl)cyclohexanecarbonitrile (270 mg, 58% yield) was prepared from (1s,4s)-1-(4-fluorophenyl)-4-hydroxycyclohexanecarbonitrile (from preparation Q) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 399.1. 1H NMR (500 MHz, DMSO-d6) δ 8.50 (d, J=6.0 Hz, 1H), 7.67-7.55 (m, 2H), 7.50 (d, J=6.0 Hz, 1H), 7.37-7.25 (m, 2H), 4.93-4.79 (m, 1H), 2.25 (d, J=10.5 Hz, 4H), 2.18-2.07 (m, 2H), 1.84-1.72 (m, 2H).
(1s,4s)-1-(4-Fluorophenyl)-4-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)cyclohexanecarbonitrile (255 mg, 0.647 mmol, 97% yield) was prepared from (1s,4s)-4-((2-chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-1-(4-fluorophenyl)cyclohexanecarbonitrile (265 mg, 0.665 mmol) (from step R1) following a procedure analogous to step A2. LC-MS (M+H)+ 395.1.
2-Chloro-4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-3-(trifluoromethyl)pyridine (257 mg, 57% yield) was prepared from (1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexanol (J. Med. Chem 1973, 16, 1251) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 404.1. 1H NMR (500 MHz, chloroform-d) δ 8.33 (d, J=6.0 Hz, 1H), 7.44-7.34 (m, 2H), 7.11-7.02 (m, 2H), 6.90 (d, J=5.8 Hz, 1H), 4.45 (tt, J=9.9, 4.8 Hz, 1H), 2.98 (s, 3H), 2.27-2.17 (m, 2H), 2.12-1.96 (m, 4H), 1.86-1.73 (m, 2H).
4-(((1s,4s)-4-(4-Fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (247 mg, 97% yield) was prepared from 2-chloro-4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-3-(trifluoromethyl)pyridine (from step S1) following a procedure analogous to step A2. LC-MS (M+H)+ 400.1.
2-Chloro-4-(((1r,4r)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridine (395 mg, 1.11 mmol, 65% yield) was prepared from (1r,4r)-4-phenylcyclohexanol (commercially available) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 356.1. 1H NMR (500 MHz, chloroform-d) δ 8.33 (d, J=5.8 Hz, 1H), 7.37-7.30 (m, 2H), 7.26-7.19 (m, 3H), 6.93 (d, J=6.0 Hz, 1H), 4.55-4.42 (m, 1H), 2.64 (tt, J=11.9, 3.5 Hz, 1H), 2.35-2.23 (m, 2H), 2.13-2.02 (m, 2H), 1.83-1.70 (m, 2H), 1.70-1.59 (m, 2H).
2-Hydrazinyl-4-(((1I,4I)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridine (385 mg, 100% yield) was prepared from 2-chloro-4-(((1r,4r)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridine (from step T1) following a procedure analogous to step A2. LC-MS (M+H)+ 352.2.
1-Fluoro-4-(prop-1-en-2-yl)benzene (3.1 g, 22.77 mmol) was added dropwise over 10 min to a solution of mCPBA (5.66 g, 25.3 mmol) in methanol (50 mL) maintained in an ice-bath. After complete addition, the mixture was allowed to warm to rt. After stirring at rt for 30 min, the reaction mixture was heated in an oil bath at 40° C. for 16 h. The reaction mixture was diluted with 50 mL of water. The mixture was concentrated in vacuo. The resulting white aqueous slurry was diluted with 1 N NaOH (50 mL) and extracted with diethyl ether. The combined organics layers were washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo. The crude product was purified using silica gel column chromatography (2:1 hexanes/ethyl acetate) to afford 2-(4-fluorophenyl)-2-methoxypropan-1-ol (2.89 g, 66% yield) as a clear oil. 1H NMR (500 MHz, chloroform-d) δ 7.40-7.33 (m, 2H), 7.10-7.02 (m, 2H), 3.63 (d, J=11.1 Hz, 1H), 3.49 (d, J=11.1 Hz, 1H), 3.14 (s, 3H), 1.61 (s, 3H).
2-Chloro-4-(2-(4-fluorophenyl)-2-methoxypropoxy)-3-(trifluoromethyl)pyridine (387 mg, 53% yield) was prepared from 2-(4-fluorophenyl)-2-methoxypropan-1-ol (from preparation U) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 364.1. 1H NMR (400 MHz, chloroform-d) δ 8.31 (d, J=6.0 Hz, 1H), 7.47-7.39 (m, 2H), 7.12-7.03 (m, 2H), 6.82 (d, J=5.8 Hz, 1H), 4.05 (dd, J=56.5, 9.0 Hz, 2H), 3.17 (s, 3H), 1.76 (s, 3H).
4-(2-(4-Fluorophenyl)-2-methoxypropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (375 mg, 100% yield) was prepared from 2-chloro-4-(2-(4-fluorophenyl)-2-methoxypropoxy)-3-(trifluoromethyl)pyridine (from step V1) following a procedure analogous to step A2. LC-MS (M+H)+ 360.1.
(1s,4s)-1-(3,4-Difluorophenyl)cyclohexane-1,4-diol (491 mg, 97% yield) was prepared from 4-(3,4-difluorophenyl)-4-hydroxycyclohexanone (U.S. Pat. No. 5,391,570) following a procedure analogous to preparation Q. The crude product was obtained as a >9:1 mixture of diasteromers and was carried forward without purification. 1H NMR (500 MHz, chloroform-d) δ 7.22 (ddd, J=12.1, 7.8, 1.8 Hz, 1H), 7.16-7.07 (m, 2H), 3.72-3.63 (m, 1H), 2.98 (s, 3H), 2.11-2.03 (m, 2H), 1.92-1.85 (m, 2H), 1.79-1.61 (m, 4H).
(1s,4s)-4-((2-chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-1-(3,4-difluorophenyl)cyclohexanol (267 mg, 50% yield) was prepared from (1s,4s)-Difluorophenyl)cyclohexane-1,4-diol (from preparation W) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 408.0. 1H NMR (500 MHz, chloroform-d) δ 8.34 (d, J=5.8 Hz, 1H), 7.35 (ddd, J=11.9, 7.6, 2.3 Hz, 1H), 7.25-7.19 (m, 1H), 7.19-7.12 (m, 1H), 6.91 (d, J=5.8 Hz, 1H), 4.56-4.42 (m, 1H), 2.20-2.10 (m, 2H), 2.10-2.03 (m, 2H), 2.01-1.90 (m, 4H).
(1s,4s)-1-(3,4-Difluorophenyl)-4-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)cyclohexanol (262 mg, quantitative yield) was prepared from (1s,4s)-4-((2-chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-1-(3,4-difluorophenyl)cyclohexanol (from step X1) following a procedure analogous to step A2. LC-MS (M+H)+ 404.1.
2-Chloro-4-(((1s,4s)-4-(3,4-difluorophenyl)-4-methoxycyclohexyl)oxy)-3-(trifluoromethyl)pyridine (260 mg, 37% yield) was prepared from (1s,4s)-4-(3,4-difluorophenyl)-4-methoxycyclohexanol (J. Med. Chem 1973, 16, 1251) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 422.1. 1H NMR (500 MHz, chloroform-d) δ 8.33 (d, J=6.0 Hz, 1H), 7.24 (ddd, J=11.9, 7.6, 2.1 Hz, 1H), 7.20-7.10 (m, 2H), 6.90 (d, J=6.0 Hz, 1H), 4.48-4.41 (m, 1H), 3.00 (s, 3H), 2.23-2.14 (m, 2H), 2.08-1.99 (m, 4H), 1.83-1.71 (m, 2H).
4-((1s,4s)-4-(3,4-Difluorophenyl)-4-methoxycyclohexyloxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (257 mg, quantitative yield) was prepared from 2-chloro-4-(((1s,4s)-4-(3,4-difluorophenyl)-4-methoxycyclohexyl)oxy)-3-(trifluoromethyl)pyridine (from step Y1) following a procedure analogous to step A2. LC-MS (M+H)+ 418.3.
2-Chloro-4-((1-(4-chlorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (438 mg, 74% yield) was prepared from (1-(4-chlorophenyl)cyclopropyl)methanol (commercially available) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 362.0. 1H NMR (500 MHz, chloroform-d) δ 8.31 (d, J=5.8 Hz, 1H), 7.39-7.35 (m, 2H), 7.32-7.29 (m, 2H), 6.75 (d, J=6.0 Hz, 1H), 4.11 (s, 2H), 1.09-1.02 (m, 4H).
4-((1-(4-Chlorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (439 mg, quantitative yield) was prepared from 2-chloro-4-((1-(4-chlorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step Z1) following a procedure analogous to step A2. LC-MS (M+H)+ 358.0.
2-Chloro-4-((1-(4-fluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (537 mg, 86% yield) was prepared from (1-(4-fluorophenyl)cyclopropyl)methanol (commercially available) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 346.0. 1H NMR (500 MHz, chloroform-d) δ 8.30 (d, J=6.0 Hz, 1H), 7.43-7.38 (m, 2H), 7.04-6.98 (m, 2H), 6.75 (d, J=5.8 Hz, 1H), 4.10 (s, 2H), 1.08-1.00 (m, 4H).
4-((1-(4-Fluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (500 mg, 94% yield) was prepared from 2-chloro-4-((1-(4-fluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AA1) following a procedure analogous to step A2. LC-MS (M+H)+ 342.0.
A mixture of trans-2-(4-chlorophenyl)cyclopropanecarboxylic acid (commercially available, 1.0 g, 5.09 mmol), methanol (29.1 ml), and sulfuric acid (0.813 ml, 15.3 mmol) was heated in a sealed vial at 72° C. for 18 h. The reaction was carefully neutralized by the addition of aqueous 10% sodium carbonate solution. The resulting mixture was evaporated in vacuo. The residue was partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate. The combined organic layers were washed with brine, dried (sodium sulfate), filtered, and concentrated in vacuo to afford methyl trans-2-(4-chlorophenyl)cyclopropanecarboxylate (1.07 g, quantitative % yield) as a light brown oil. 1H NMR (500 MHz, chloroform-d) δ 7.29-7.26 (m, 2H), 7.07-7.03 (m, 2H), 3.74 (s, 3H), 2.52 (ddd, J=9.3, 6.4, 4.2 Hz, 1H), 1.89 (ddd, J=8.5, 5.3, 4.3 Hz, 1H), 1.65-1.60 (m, 1H), 1.33-1.29 (m, 1H).
To a stirring suspension of LAH (0.589 g, 15.5 mmol) in THF (2.5 mL) was added dropwise a solution of methyl 2-(4-chlorophenyl)cyclopropanecarboxylate (from step AB1, 1.07 g, 5.07 mmol) in THF (5 mL). The resulting mixture was stirred at rt for 18 h. The reaction was quenched by the addition of aqueous ammonium hydroxide solution. The reaction mixture was slurried in a bilayer of water and ethyl acetate and filtered through celite. The layers were separated and the aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo. The residue was purified by silica-gel column chromatography (0-35% ethyl acetate/hexane) to afford (trans-2-(4-chlorophenyl)cyclopropyl)methanol (582 mg, 3.19 mmol, 62.8% yield). LC-MS (M−H2O+H)+ 165.0.
(±)-2-Chloro-4-(((1S,2S)-2-(4-chlorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (450 mg, 76% yield) was prepared from (trans-2-(4-chlorophenyl)cyclopropyl)methanol (from preparation AB) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.38 (d, J=4.7 Hz, 1H), 7.28-7.25 (m, 2H), 7.09-7.05 (m, 2H), 6.89 (d, J=4.6 Hz, 1H), 4.27 (dd, J=9.8, 6.1 Hz, 1H), 4.16-4.07 (m, 1H), 2.07-2.01 (m, 1H), 1.56 (dd, J=12.6, 6.3 Hz, 1H), 1.18-1.10 (m, 2H).
4-((1-(4-Fluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (451 mg, quantitative yield) was prepared from (±)-2-chloro-4-(((1S,2S)-2-(4-chlorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AC1) following a procedure analogous to step A2. LC-MS (M+H)+ 358.0.
A solution of (E)-3-(2-fluoro-6-methoxyphenyl)acrylic acid (commercially available, 1.8 g, 9.18 mmol) in thionyl chloride (30 mL) was heated to reflux for 2 h. The resulting mixture was concentrated in vacuo to afford a tan solid. The solid was dissolved in ethyl acetate (50 mL) and added to a solution of N,O-dimethylhydroxylamine, HCl (1.1 g, 11.3 mmol) and potassium carbonate (3.80 g, 27.5 mmol) in water (50 mL). The mixture was stirred for 1 hr. The ethyl acetate layer was separated, washed with water, washed with brine, and concentrated to afford (E)-3-(2-fluoro-6-methoxyphenyl)-N-methoxy-N-methylacrylamide as a brown oil (2.15 g, 98% yield). LC-MS (M+H)+ 240.1.
A 60% dispersion of sodium hydride in mineral (3.01 g, 75 mmol) was added in 1 g portions to vigorously stirred DMSO (75 mL). After the foaming subsided, trimethylsulfoxonium iodide (16.6 g, 75 mmol) was added portion wise over 1 h, while maintaining the internal temperature below 35° C. After an additional 30 min, a solution of (E)-3-(2-fluoro-6-methoxyphenyl)-N-methoxy-N-methylacrylamide (from step AD1, 6.0 g, 25 mmol) in DMSO (50 mL) was added dropwise while still maintaining the internal temperature between 30-35° C. The resulting mixture was stirred for 1.5 h at rt. The rxn was quenched with aqueous ammonium chloride solution (25 mL). The resulting mixture was diluted with EtOAc and washed with water. The organic layer was washed with brine, dried (magnesium sulfate), filtered, and concentrated in vacuo to afford to afford trans-2-(2-fluoro-6-methoxyphenyl)-N-methoxy-N-methylcyclopropanecarboxamide (8.1 g, quantitative yield) as a clear oil. LC-MS (M+H)+ 254.1.
trans-2-(2-Fluoro-6-methoxyphenyl)-N-methoxy-N-methylcyclopropanecarboxamide (from step AD2, 7.0 g, 27.6 mmol) was treated at rt with a solution of 2.5 M aqueous sodium hydroxide (75 ml, 188 mmol). The reaction mixture was stirred at rt for 18 h and heated at reflux for and additional 1.5 h. The reaction mixture was diluted with water, and extracted with DCM. The aqueous layer was made acidic with concentrated hydrochloric acid. The resulting white precipitate was collected and dried under high vacuum to afford trans-2-(2-fluoro-6-methoxyphenyl)cyclopropanecarboxylic acid (4.4 g, 76% yield) as a white solid. 1H NMR (400 MHz, chloroform-d) δ 7.15 (td, J=8.3, 6.5 Hz, 1H), 6.72-6.60 (m, 2H), 3.86 (s, 3H), 2.59 (ddd, J=9.5, 7.0, 4.5 Hz, 1H), 2.15 (dt, J=7.8, 5.0 Hz, 1H), 1.68 (td, J=7.5, 4.5 Hz, 1H), 1.65-1.57 (m, 1H).
trans-methyl 2-(2-fluoro-6-methoxyphenyl)cyclopropanecarboxylate (395 mg, 99% yield) was prepared from trans-2-(2-fluoro-6-methoxyphenyl)cyclopropanecarboxylic acid (from step AD3) following a procedure analogous to step AB1. LC-MS (M+H)+ 225.0. 1H NMR (500 MHz, chloroform-d) δ 7.13 (td, J=8.3, 6.6 Hz, 1H), 6.67-6.61 (m, 2H), 3.84 (s, 3H), 3.76-3.74 (m, 3H), 2.58-2.49 (m, 1H), 2.18 (dt, J=8.2, 4.9 Hz, 1H), 1.60 (dt, J=7.6, 3.8 Hz, 1H), 1.54-1.51 (m, 1H).
(trans-2-(2-fluoro-6-methoxyphenyl)cyclopropyl)methanol (175 mg, 51% yield) was prepared from trans-methyl 2-(2-fluoro-6-methoxyphenyl)cyclopropanecarboxylate (from step AD4) following a procedure and purification method analogous to step AB2. LCMS (M−H2O+H)+ 179.0. 1H NMR (500 MHz, chloroform-d) δ 7.14 (td, J=8.4, 6.5 Hz, 1H), 6.70-6.63 (m, 2H), 3.96-3.86 (m, 4H), 3.31 (dd, J=10.8, 8.9 Hz, 1H), 1.60 (dt, J=8.9, 5.3 Hz, 1H), 1.47-1.39 (m, 1H), 1.31-1.23 (m, 1H), 0.93 (dt, J=9.0, 5.3 Hz, 1H).
(±)-2-Chloro-4-(((1S,2S)-2-(2-fluoro-6-methoxyphenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (298 mg, 89% yield) was prepared from (trans-2-(2-fluoro-6-methoxyphenyl)cyclopropyl)methanol (from preparation AD) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.36 (d, J=6.0 Hz, 1H), 7.13 (td, J=8.3, 6.4 Hz, 1H), 6.93 (d, J=5.8 Hz, 1H), 6.71-6.61 (m, 2H), 4.33 (dd, J=10.1, 5.9 Hz, 1H), 4.14 (dd, J=10.1, 6.6 Hz, 1H), 3.83 (s, 3H), 1.94 (dt, J=9.2, 5.5 Hz, 1H), 1.83 (dt, J=8.2, 5.9 Hz, 1H), 1.34 (dt, J=8.4, 5.5 Hz, 1H), 1.11-1.02 (m, 1H).
(±)-4-(((1S,2S)-2-(2-Fluoro-6-methoxyphenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (294 mg, quantitative yield) was prepared from (±)-2-chloro-4-(((1S,2S)-2-(2-fluoro-6-methoxyphenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AE1) following a procedure analogous to step A2. LC-MS (M+H)+ 371.1.
trans-Methyl 2-(2-methoxyphenyl)cyclopropanecarboxylate (1.07 g, quantitative yield) was prepared from trans-methyl 2-(2-methoxyphenyl)cyclopropanecarboxylic (commercially available) following a procedure analogous to step AB1. LC-MS (M+H)+ 206.4.
(trans-2-(2-Methoxyphenyl)cyclopropyl)methanol (959 mg, quantitative yield) was prepared from trans-methyl 2-(2-methoxyphenyl)cyclopropanecarboxylate (from step AF1) following a procedure analogous to step AB2. 1H NMR (400 MHz, chloroform-d) δ 7.24-7.18 (m, 1H), 7.01-6.97 (m, 1H), 6.94-6.85 (m, 2H), 3.91 (s, 3H), 3.90-3.87 (m, 1H), 3.31 (dd, J=10.8, 8.8 Hz, 1H), 1.92 (dt, J=8.6, 5.2 Hz, 1H), 1.26-1.19 (m, 1H), 1.14-1.07 (m, 1H), 0.89 (dt, J=8.7, 5.1 Hz, 1H).
(±)-2-Chloro-4-(((1S,2S)-2-(2-methoxyphenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (360 mg, 60% yield) was prepared from (trans-2-(2-methoxyphenyl)cyclopropyl)methanol (from preparation AF) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.36 (d, J=5.8 Hz, 1H), 7.20 (ddd, J=8.1, 7.1, 2.1 Hz, 1H), 6.94-6.87 (m, 4H), 4.30 (dd, J=10.1, 6.3 Hz, 1H), 4.13 (dd, J=10.1, 6.9 Hz, 1H), 3.86 (s, 3H), 2.25 (dt, J=8.9, 5.3 Hz, 1H), 1.55-1.49 (m, 1H), 1.14 (dt, J=8.4, 5.5 Hz, 1H), 1.06 (dt, J=9.0, 5.3 Hz, 1H).
(±)-2-Hydrazinyl-4-(((1S,2S)-2-(2-methoxyphenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (312 mg, 88% yield) was prepared from (±)-2-chloro-4-(((1S,2S)-2-(2-methoxyphenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AG1) following a procedure analogous to step A2. LC-MS (M+H)+ 354.1.
trans-Methyl 2-(2-trifluoromethoxyphenyl)cyclopropanecarboxylate (0.700 mg, 80% yield) was prepared from trans-methyl 2-(2-trifluoromethoxyphenyl)cyclopropanecarboxylic (commercially available) following a procedure analogous to step AB1. LC-MS (M+H)+ 261.1. 1H NMR (400 MHz, chloroform-d) δ 7.29-7.21 (m, 3H), 7.06-7.00 (m, 1H), 3.76 (s, 3H), 2.74 (ddd, J=9.3, 6.7, 4.4 Hz, 1H), 1.90 (ddd, J=8.4, 5.3, 4.4 Hz, 1H), 1.71-1.61 (m, 1H), 1.40-1.27 (m, 1H).
(trans-2-(2-Trifluoromethoxyphenyl)cyclopropyl)methanol (210 mg, 34% yield) was prepared from trans-methyl 2-(2-trifluoromethoxyphenyl)cyclopropanecarboxylate (from step AH1) following a procedure analogous to step AB2.
(±)-2-Hydrazinyl-4-(((1S,2S)-2-(2-(trifluoromethoxy)phenyl)-cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (312 mg, 42% yield over 2 steps) was prepared from (trans-2-(2-trifluoromethoxyphenyl)-cyclopropyl)methanol (from preparation AH) following procedures analogous to steps A1 and A2. LC-MS (M+H)+ 408.1.
(trans-2-(2-Fluorophenyl)cyclopropyl)methanol (280 mg, 34% yield) was prepared from trans-2-(2-fluorophenyl)cyclopropanecarboxylic acid (commercially available) following the procedures analogous to step AB1 and AB2. LC-MS (M+H)+ 149.2. 1H NMR (500 MHz, chloroform-d) δ 7.19-7.13 (m, 1H), 7.09-7.00 (m, 2H), 6.96 (td, J=7.6, 1.5 Hz, 1H), 3.76-3.68 (m, 1H), 3.66-3.59 (m, 1H), 2.04-1.98 (m, 1H), 1.52-1.44 (m, 2H), 1.08-1.02 (m, 1H), 0.97 (dt, J=8.9, 5.2 Hz, 1H).
(trans-2-(2,5-Difluorophenyl)cyclopropyl)methanol was prepared from trans-2-(2,5-difluorophenyl)cyclopropanecarboxylic acid (commercially available) following the procedures analogous to step AB1 and AB2. LC-MS (M+H)+ 167.1 1H NMR (500 MHz, chloroform-d) δ 6.97 (td, J=9.2, 4.7 Hz, 1H), 6.86-6.79 (m, 1H), 6.62 (ddd, J=9.2, 6.0, 3.1 Hz, 1H), 3.75-3.68 (m, 1H), 3.65-3.59 (m, 1H), 2.04-1.98 (m, 1H), 1.52 (t, J=5.0 Hz, 1H), 1.49-1.42 (m, 1H), 1.05-0.98 (m, 2H).
(trans-2-(4-Methoxyphenyl)cyclopropyl)methanol was prepared from trans-2-(4-methoxyphenyl)cyclopropanecarboxylic acid (commercially available) following the procedures analogous to step AB1 and AB2. LC-MS (M−H2O+H)+=161.2. 1H NMR (500 MHz, chloroform-d) δ 7.10-6.98 (m, 2H), 6.92-6.77 (m, 2H), 3.84-3.77 (m, 3H), 3.70-3.56 (m, 2H), 1.87-1.76 (m, 1H), 1.45-1.34 (m, 2H), 0.96-0.86 (m, 2H).
(1s,3s)-3-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(3-fluorophenyl)cyclobutanecarbonitrile (298 mg, 67% yield) was prepared from (1s,3s)-1-(3-fluorophenyl)-3-hydroxycyclobutanecarbonitrile (WO 2003/063797) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LCMS (M+H)+371.0. 1H NMR (500 MHz, chloroform-d) δ 8.37 (d, J=5.6 Hz, 1H), 7.48 (td, J=8.1, 6.0 Hz, 1H), 7.32-7.09 (m, 4H), 6.62 (d, J=5.8 Hz, 1H), 4.96 (t, J=6.6 Hz, 1H), 3.24 (d, J=6.6 Hz, 4H).
(1s,3s)-1-(3-Fluorophenyl)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclobutanecarbonitrile (294 mg, quantitative yield) was prepared from (1s,3s)-3-(2-chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(3-fluorophenyl)cyclobutanecarbonitrile (from step AM1) following a procedure analogous to step A2. LC-MS (M+H)+ 367.1.
A 60% dispersion of sodium hydride in mineral oil (0.906 g, 37.8 mmol) was added to a chilled 0° C. solution of diethyl (2-(methoxy(methyl)amino)-2-oxoethyl)phosphonate (7.64 g, 32.0 mmol) in diethyl ether (250 mL). After 30 minutes, 3,6-difluoro-2-methoxybenzaldehyde (5.0 g, 29.0 mmol) was slowly added. The resulting mixture was allowed to warm to rt and stir for 18 h. The reaction was quenched with 1N HCl (10 mL), then poured into water. The aqueous layer was made acidic with 1N HCl and extracted with ethyl acetate. The combined organic layers were washed with brine, dried over sodium sulfate, filtered and concentrated in vacuo. The crude product mixture was purified by silica-gel column chromatography (0-50% EtOAc/hexanes) to afford (E)-3-(3,6-difluoro-2-methoxyphenyl)-N-methoxy-N-methylacrylamide (4.96 g, 66% yield). LC-MS (M+H)+ 258.1.
trans-2-(3,6-Difluoro-2-methoxyphenyl)-N-methoxy-N-methylcyclopropanecarboxamide (2.12 g, quantitative yield) was prepared from trans-3-(3,6-difluoro-2-methoxyphenyl)-N-methoxy-N-methylacrylamide (from step AN1) following a procedure analogous to step AD2. LC-MS (M+H)+ 272.1. 1H NMR (500 MHz, chloroform-d) δ 6.98-6.85 (m, 1H), 6.69 (td, J=9.5, 4.0 Hz, 1H), 3.94 (s, 3H), 3.77 (s, 3H), 3.27 (s, 3H), 1.71 (br. s., 1H), 1.59-1.50 (m, 2H), 0.91-0.83 (m, 1H).
trans-2-(3,6-difluoro-2-methoxyphenyl)cyclopropanecarboxylic acid (1.38 g, 78% yield) was prepared from trans-2-(3,6-Difluoro-2-methoxyphenyl)-N-methoxy-N-methylcyclopropanecarboxamide (from step AN2) following a procedure analogous to step AD3. 1H NMR (500 MHz, chloroform-d) δ 6.93 (ddd, J=10.7, 9.2, 5.1 Hz, 1H), 6.70 (td, J=9.5, 4.0 Hz, 1H), 3.98 (s, 3H), 2.62 (ddd, J=9.5, 7.1, 4.4 Hz, 1H), 2.18 (dt, J=7.8, 5.1 Hz, 1H), 1.71-1.62 (m, 2H).
(trans-2-(3,6-Difluoro-2-methoxyphenyl)cyclopropyl)methanol (1.15 g, 5.37 mmol, 89% yield) was prepared from trans-2-(3,6-difluoro-2-methoxyphenyl)cyclopropanecarboxylic acid (from step AN3) following a procedure and purification method analogous to step F2. LC-MS (M−H2O+H)+ 197.1. 1H NMR (500 MHz, chloroform-d) δ 6.89 (ddd, J=10.7, 9.3, 5.0 Hz, 1H), 6.68 (td, J=9.5, 4.0 Hz, 1H), 3.99 (s, 3H), 3.82 (dd, J=11.0, 6.0 Hz, 1H), 3.48 (dd, J=11.1, 7.9 Hz, 1H), 1.77-1.69 (m, 1H), 1.59 (dtd, J=8.2, 5.3, 2.4 Hz, 1H), 1.33-1.25 (m, 1H), 0.97-0.91 (m, 1H).
(trans-2-(2-Chloro-6-fluorophenyl)cyclopropyl)methanol (1.12 g, 42% yield over 2 steps) was prepared from trans-2-(2-chloro-6-fluorophenyl)cyclopropanecarboxylic acid (commercially available) following procedures analogous to step AB1 and AB2. LC-MS (M−H2O+H)+=183.1. 1H NMR (500 MHz, chloroform-d) δ 7.20-7.16 (m, 1H), 7.15-7.10 (m, 1H), 6.94 (ddd, J=10.3, 8.3, 1.2 Hz, 1H), 3.77-3.69 (m, 2H), 1.75 (dt, J=9.1, 5.3 Hz, 1H), 1.62-1.57 (m, 2H), 1.22-1.16 (m, 1H), 1.04 (dtd, J=9.1, 5.4, 1.2 Hz, 1H).
(E)-3-(4-fluoro-2-methoxyphenyl)-N-methoxy-N-methylacrylamide (4.96 g, 64% yield) was prepared from 4-fluoro-2-methoxybenzaldehyde (commercially available) following a procedure analogous to step AN1. LC-MS (M+H)+ 240.1. 1H NMR (400 MHz, chloroform-d) δ 7.95 (d, J=15.8 Hz, 1H), 7.52 (dd, J=8.4, 6.7 Hz, 1H), 7.04 (d, J=15.8 Hz, 1H), 6.71-6.58 (m, 2H), 3.87 (s, 3H), 3.76 (s, 3H), 3.30 (s, 3H).
trans-2-(4-Fluoro-2-methoxyphenyl)-N-methoxy-N-methylcyclopropanecarboxamide (870 mg, 41% yield) was prepared from (E)-3-(4-fluoro-2-methoxyphenyl)-N-methoxy-N-methylacrylamide (from step AP1) following a procedure analogous to step AD2. LC-MS (M+H)+ 254.1. 1H NMR (500 MHz, chloroform-d) δ 6.92 (dd, J=9.2, 6.6 Hz, 1H), 6.62-6.56 (m, 2H), 3.83 (s, 3H), 3.73 (s, 3H), 3.27 (s, 3H), 2.63 (ddd, J=9.1, 6.6, 4.5 Hz, 1H), 2.30 (br. s., 1H), 1.61-1.58 (m, 1H), 1.30-1.28 (m, 1H).
trans-2-(4-Fluoro-2-methoxyphenyl)cyclopropanecarboxylic acid (371 mg, 51% yield) was prepared from trans-2-(4-Fluoro-2-methoxyphenyl)-N-methoxy-N-methylcyclopropanecarboxamide (from step AP2) following a procedure analogous to step AD3. 1H NMR (500 MHz, chloroform-d) δ 6.89 (dd, J=7.9, 6.6 Hz, 1H), 6.65-6.54 (m, 2H), 3.86 (s, 3H), 2.72 (ddd, J=9.2, 6.8, 4.4 Hz, 1H), 1.82-1.76 (m, 1H), 1.64-1.61 (m, 1H), 1.39 (ddd, J=8.2, 6.9, 4.5 Hz, 1H).
(trans-2-(4-Fluoro-2-methoxyphenyl)cyclopropyl)methanol (367 mg, quantitative yield) was prepared from trans-2-(4-fluoro-2-methoxyphenyl)cyclopropanecarboxylic acid (from step AP3) following a procedure and purification method analogous to step F2. LC-MS (M−H2O+H)+ 179.1.
(1s,3s)-3-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(4-fluorophenyl)cyclobutanecarbonitrile (370 mg, 20% yield) was prepared from (1s,3s)-1-(4-fluorophenyl)-3-hydroxycyclobutanecarbonitrile (4:1 trans/cis mixture, WO 2009/156100) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LCMS (M+H)+371.0. 1H NMR (500 MHz, chloroform-d) δ 8.37 (d, J=5.8 Hz, 1H), 7.51-7.45 (m, 2H), 7.23-7.16 (m, 2H), 6.61 (d, J=5.8 Hz, 1H), 4.94 (t, J=6.6 Hz, 1H), 3.24 (d, J=6.6 Hz, 4H).
(1s,3s)-1-(4-Fluorophenyl)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclobutanecarbonitrile (368 mg, quantitative yield) was prepared from (1s,3s)-3-(2-chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(4-fluorophenyl)cyclobutanecarbonitrile (from step AQ1) following a procedure analogous to step A2. LC-MS (M+H)+ 367.1.
(1-(2,4-Difluorophenyl)cyclopropyl)methanol (810 mg, 87% yield) was prepared from 1-(2,4-difluorophenyl)cyclopropanecarboxylic acid (Angew. Chem., Int. Ed. 2011, 50, 314) following a procedure analogous to step F2. LC-MS (M−H2O+H)+ 167.1.
2-Chloro-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (1.0, 99% yield) was prepared from ((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methanol (from preparation C) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 346.1.
4-(((1S,2S)-2-(4-Fluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (342 mg, 97% yield) was prepared from 2-chloro-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AS1) following a procedure analogous to step A2. LC-MS (M+H)+ 342.1.
To a solution of 2-(2-methoxyphenyl)acetonitrile (5.0 g, 34.0 mmol) in THF (100 mL) was added a solution of methyllithium (1.6 M in hexane, 21.2 mL, 34.0 mmol) dropwise and the resulting solution was stirred for 1 h at −78° C. To the solution was added 2-(bromomethyl)oxirane (2.91 mL, 34.0 mmol) in THF (50 mL) dropwise over 30 min and the reaction mixture was stirred for 1 h at −78° C. A solution of methylmagnesium bromide (3.0 M in THF, 11.3 mL, 34.0 mmol) was added into the reaction mixture and the resulting mixture was stirred for 12 h at room temperature. The reaction mixture was diluted with ethyl acetate and washed with brine. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. The oily residue was purified by silica gel column chromatography (0-100% ethyl acetate/hexanes) to afford (1s,3s)-3-hydroxy-1-(2-methoxyphenyl)cyclobutanecarbonitrile (4.61 g, 67% yield) as a 4:1 ((1s,3s)/(1s,3r)) mixture of diastereomers. Data for (1s,3s) isomer only: 1H NMR (500 MHz, chloroform-d) δ 7.39-7.29 (m, 2H), 7.02-6.95 (m, 2H), 4.34-4.26 (m, 1H), 3.94 (s, 3H), 3.10-3.03 (m, 2H), 2.86-2.79 (m, 2H).
(1s,3s)-3-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(2-methoxyphenyl)cyclobutanecarbonitrile (856 mg, 46% yield) was prepared from (1s,3s)-3-hydroxy-1-(2-methoxyphenyl)cyclobutanecarbonitrile (from preparation AT) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 383.0. 1H NMR (500 MHz, chloroform-d) δ 8.32 (dd, J=5.6, 1.8 Hz, 1H), 7.43-7.37 (m, 1H), 7.33-7.27 (m, 1H), 7.06-6.98 (m, 2H), 6.60 (d, J=5.8 Hz, 1H), 4.79 (t, J=6.0 Hz, 1H), 3.94 (s, 3H), 3.32-3.24 (m, 2H), 3.18-3.10 (m, 2H).
(1s,3s)-3-(2-Hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)-1-(2-methoxyphenyl)cyclobutanecarbonitrile (820 mg, 97% yield) was prepared from (1s,3s)-3-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(2-methoxyphenyl)cyclobutanecarbonitrile (from step AU1) following a procedure analogous to step A2. LC-MS (M+H)+ 379.1.
(1s,3s)-1-(3,5-Difluorophenyl)-3-hydroxycyclobutanecarbonitrile (3.87 g, 57% yield) was prepared from 2-(3,5-difluorophenyl)acetonitrile following a procedure analogous to preparation AT. The product was isolated as a 4:1 ((1s,3s)/(1s,3r)) mixture of diastereomers. Data for (1s,3s) isomer only: 1H NMR (500 MHz, chloroform-d) δ 7.02-6.99 (m, 2H), 6.85-6.80 (m, 1H), 4.57-4.47 (m, 1H), 3.02-2.90 (m, 4H), 2.18 (d, J=5.6 Hz, 1H).
(1s,3s)-3-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(3,5-difluorophenyl)cyclobutanecarbonitrile (427 mg, 23% yield) was prepared from (1s,3s)-1-(3,5-difluorophenyl)-3-hydroxycyclobutanecarbonitrile (from preparation AV) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LCMS (M+H)+389.0.
(1s,3s)-1-(3,5-Difluorophenyl)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclobutanecarbonitrile (405 mg, 96% yield) was prepared from (1s,3s)-3-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(3,5-difluorophenyl)cyclobutanecarbonitrile (from step AW1) following a procedure analogous to step A2. LC-MS (M+H)+ 385.1.
(1-(3,5-Difluorophenyl)cyclopropyl)methanol (860 mg, 93% yield) was prepared from 1-(3,5-difluorophenyl)cyclopropanecarboxylic acid (commercially available) following a procedure analogous to step F2. LC-MS (M−H2O+H)+ 167.1.
2-Chloro-4-((1-(3,5-difluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (943 mg, 56% yield) was prepared from (1-(3,5-difluorophenyl)cyclopropyl)methanol (from preparation AX) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LCMS (M+H)+364.0.
4-((1-(3,5-Difluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (850 mg, 91% yield) was prepared from 2-chloro-4-((1-(3,5-difluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AY1) following a procedure analogous to step A2. LC-MS (M+H)+ 360.2.
(1-(3,5-Dichlorophenyl)cyclopropyl)methanol (660 mg, 70% yield) was prepared from 1-(3,5-dichlorophenyl)cyclopropanecarboxylic acid (commercially available) following a procedure analogous to step F2. LC-MS (M−H2O+H)+ 199.1.
2-Chloro-4-((1-(3,5-dichlorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (856 mg, 71% yield) was prepared from (1-(3,5-dichlorophenyl)cyclopropyl)methanol (from preparation AZ) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LCMS (M+H)+ 396.0.
4-((1-(3,5-Dichlorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (800 mg, 95% yield) was prepared from 2-chloro-4-((1-(3,5-dichlorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AAA1) following a procedure analogous to step A2. LC-MS (M+H)+ 392.1.
Sodium borohydride (108 mg, 2.86 mmol) was added to a stirred solution of 3-phenylcyclobutanone (380 mg, 2.60 mmol) in ethanol (15 mL) maintained in a 0° C. ice bath. After 2 h, the reaction was diluted with water and the pH was adjusted to 2 by the addition of 1 N aqueous hydrochloric acid. The mixture was extracted with ethyl acetate. The combined organics were washed with brine, dried (magnesium sulfate), filtered and concentrated in vacuo to afford (1r,3r)-3-phenylcyclobutanol (423 mg, quantitative yield). The product was used without purification. 1H NMR was consistent with the desired product, but suggested a 2:1 (trans/cis) mixture of diastereomers. Data major diastereomer: 1H NMR (500 MHz, chloroform-d) δ 7.33-7.31 (m, 2H), 7.25 (d, J=7.8 Hz, 3H), 4.35-4.28 (m, 1H), 3.03-2.95 (m, 1H), 2.84-2.77 (m, 2H), 2.07-2.01 (m, 2H).
2-Chloro-4-((1r,3r)-3-phenylcyclobutoxy)-3-(trifluoromethyl)pyridine (100 mg, 11% yield) was prepared from (1-(3,5-dichlorophenyl)cyclopropyl)methanol (from preparation AAB) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LCMS (M+H)+ 328.2. 1H NMR (500 MHz, chloroform-d) δ 8.36 (d, J=5.8 Hz, 1H), 7.39-7.33 (m, 2H), 7.28-7.24 (m, 3H), 6.81 (d, J=5.8 Hz, 1H), 4.82 (quin, J=7.2 Hz, 1H), 3.31-3.22 (m, 1H), 3.06-2.97 (m, 2H), 2.41 (tdq, J=9.9, 7.5, 2.5 Hz, 2H).
2-Hydrazinyl-4-((1r,3r)-3-phenylcyclobutoxy)-3-(trifluoromethyl)pyridine (103 mg, quantitative yield) was prepared from 2-chloro-4-((1r,3r)-3-phenylcyclobutoxy)-3-(trifluoromethyl)pyridine (from step AAC1) following a procedure analogous to step A2. LC-MS (M+H)+ 324.2.
4-(Benzyloxy)-2-chloro-3-(trifluoromethyl)pyridine (508 mg, 72% yield) was prepared from benzylalcohol (commercially available) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.33 (d, J=5.8 Hz, 1H), 7.46-7.35 (m, 5H), 6.95 (d, J=5.3 Hz, 1H), 5.27 (s, 1H).
4-(Benzyloxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (500 mg, quantitative yield) was prepared from 4-(benzyloxy)-2-chloro-3-(trifluoromethyl)pyridine (from step AAD1) following a procedure analogous to step A2. LC-MS (M+H)+ 284.0.
7-(Benzyloxy)-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (205 mg, 33% yield over 2 steps) was prepared from 4-(benzyloxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from step AAD2) following procedures analogous to steps 1A and 1B of example 1. The final product was purified by silica gel column chromatography (100% EtOAc). LC-MS (M+H)+ 348.2. 1H NMR (400 MHz, methanol-d4) δ 8.97 (d, J=7.8 Hz, 1H), 7.75 (d, J=7.8 Hz, 1H), 7.58-7.50 (m, 2H), 7.50-7.37 (m, 3H), 5.66 (s, 2H), 3.14 (d, J=6.8 Hz, 2H), 1.41-1.25 (m, 1H), 0.75-0.68 (m, 2H), 0.42 (q, J=4.9 Hz, 2H).
A mixture of 5% palladium-on-charcoal (23 mg, 7-(benzyloxy)-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from step AAD3, 203 mg, 0.58 mmol), and methanol (15 mL) was subjected to a balloon hydrogenation for 21 days. The hydrogen atmosphere was removed under vacuum and the catalyst was filtered off and washed with dichloromethane. The filtrate was concentrated in vacuo. The residue was purified using silica gel column chromatography (5% methanol/ethyl acetate) to afford 3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-ol (104 mg, 69% yield) as a white solid. LC-MS (M+H)+ 258.1. 1H NMR (500 MHz, Methanol-d4) δ 8.18 (d, J=7.8 Hz, 1H), 6.56 (d, J=7.8 Hz, 1H), 2.94 (d, J=6.9 Hz, 2H), 1.30-1.18 (m, 1H), 0.72-0.62 (m, 2H), 0.42-0.31 (m, 2H).
trans-Methyl 2-(4-fluorophenyl)cyclopropanecarboxylate (167 mg, 97% yield) was prepared from trans-2-(4-fluorophenyl)cyclopropanecarboxylic acid (commercially available) following a procedure analogous to step AB1. 1H NMR (500 MHz, chloroform-d) δ 7.11-7.06 (m, 2H), 7.01-6.95 (m, 2H), 3.74 (s, 3H), 2.53 (ddd, J=9.3, 6.4, 4.1 Hz, 1H), 1.87 (ddd, J=8.4, 5.2, 4.2 Hz, 1H), 1.64-1.58 (m, 1H), 1.32-1.25 (m, 2H).
(trans-2-(4-fluorophenyl)cyclopropyl)methanol (119 mg, 83% yield) was prepared from trans-methyl 2-(4-fluorophenyl)cyclopropanecarboxylate (from step AAE1) following a procedure analogous to step AB2. 1H NMR (500 MHz, chloroform-d) δ 7.10-7.01 (m, 2H), 6.98-6.93 (m, 2H), 3.63 (d, J=6.7 Hz, 2H), 1.86-1.80 (m, 1H), 1.68 (br. s., 1H), 1.45-1.37 (m, 1H), 0.97-0.85 (m, 3H).
(±)-2-chloro-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (183 mg, 76% yield) was prepared from (trans-2-(4-fluorophenyl)cyclopropyl)methanol (from preparation AAE) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.34 (d, J=5.8 Hz, 1H), 7.12-7.07 (m, 2H), 7.01-6.94 (m, 2H), 6.88 (d, J=5.8 Hz, 1H), 4.26 (dd, J=9.9, 6.0 Hz, 1H), 4.07 (dd, J=9.8, 6.9 Hz, 1H), 1.57-1.49 (m, 1H), 1.16-1.06 (m, 2H), 0.96-0.83 (m, 1H).
(±)-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (181 mg, quantitative yield) was prepared from (±)-2-chloro-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from step AAF1) following a procedure analogous to step A2. LC-MS (M+H)+ 342.1. 1H NMR (500 MHz, Methanol-d4) δ 8.19-8.16 (m, 1H), 7.17-7.13 (m, 2H), 6.98 (s, 2H), 6.59-6.53 (m, 1H), 4.29-4.23 (m, 1H), 4.10-4.03 (m, 1H), 1.55-1.46 (m, 1H), 1.11-1.04 (m, 2H), 19F NMR m-56.19, -119.87.
2-Chloro-4-(2-chloro-6-fluorophenethoxy)-3-(trifluoromethyl)pyridine (241 mg, 58% yield) was prepared from 2-(2-chloro-6-fluorophenyl)ethanol (commercially available) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 355.8.
4-(2-Chloro-6-fluorophenethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (142 mg, 68% yield) was prepared from 2-chloro-4-(2-chloro-6-fluorophenethoxy)-3-(trifluoromethyl)pyridine (from step AAF1) following a procedure analogous to step A2. LC-MS (M+H)+ 350.0.
Methyl 2-(2,6-difluorophenyl)acetate (331 g, 60% yield) was prepared from 2-(2,6-difluorophenyl)acetic acid (commercially available) following a procedure analogous to step AB1. 1H NMR (500 MHz, chloroform-d) δ 7.30-7.20 (m, 1H), 6.95-6.87 (m, 2H), 3.77-3.71 (m, 5H).
2-(2,6-Difluorophenyl)ethanol (165 mg, 59% yield) was prepared from methyl methyl 2-(2,6-difluorophenyl)acetate (from step AAH1) following a procedure analogous to step AB2. 1H NMR (400 MHz, chloroform-d) δ 7.14 (tt, J=8.4, 6.4 Hz, 1H), 6.88-6.78 (m, 2H), 3.78 (t, J=6.1 Hz, 2H), 2.92 (t, J=6.9 Hz, 2H), 2.34 (br. s., 1H).
2-Chloro-4-(2,6-difluorophenethoxy)-3-(trifluoromethyl)pyridine (243 mg, 69% yield) was prepared from 2-(2,6-difluorophenyl)ethanol (from preparation AAH) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+ 338.02. 1H NMR (500 MHz, chloroform-d) δ 8.34 (d, J=5.8 Hz, 1H), 7.28-7.18 (m, 1H), 6.96-6.84 (m, 3H), 4.33 (t, J=6.8 Hz, 2H), 3.29-3.22 (m, 2H).
4-(2,6-Difluorophenethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (230 mg, 96% yield) was prepared from 2-chloro-4-(2,6-difluorophenethoxy)-3-(trifluoromethyl)pyridine (from step AAI1) following a procedure analogous to step A2. LC-MS (M+H)+ 334.1. 1H NMR (500 MHz, chloroform-d) δ 8.15 (d, J=6.0 Hz, 1H), 7.26-7.16 (m, 1H), 6.94-6.85 (m, 2H), 6.52 (d, J=2.0 Hz, 1H), 6.34 (d, J=5.8 Hz, 1H), 4.26 (t, J=6.9 Hz, 2H), 4.01 (br. s., 2H), 3.21 (t, J=6.9 Hz, 2H).
2,2-difluoro-2-phenylethanol (179 mg, 66% yield) was prepared from ethyl 2,2-difluoro-2-phenylacetate (commercially available) following a procedure analogous to step AB2. 1H NMR (400 MHz, chloroform-d) δ 7.58-7.36 (m, 5H), 3.98 (td, J=13.4, 6.0 Hz, 2H), 2.14 (t, J=6.4 Hz, 1H).
2-Chloro-4-(2,2-difluoro-2-phenylethoxy)-3-(trifluoromethyl)pyridine (221 mg, 58% yield) was prepared from 2,2-difluoro-2-phenylethanol (from preparation AAJ) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.37 (d, J=5.8 Hz, 1H), 7.62-7.55 (m, 2H), 7.54-7.45 (m, 3H), 6.85 (d, J=5.8 Hz, 1H), 4.49 (t, J=11.1 Hz, 2H).
4-(2,2-Difluoro-2-phenylethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (186 mg, 86% yield) was prepared from 2-chloro-4-(2,2-difluoro-2-phenylethoxy)-3-(trifluoromethyl)pyridine (from step AAI1) following a procedure analogous to step A2. 1H NMR (500 MHz, chloroform-d) δ 8.23-8.12 (m, 1H), 7.62-7.43 (m, 5H), 6.54 (br s, 1H), 6.28 (d, J=6.0 Hz, 1H), 4.43 (t, J=11.1 Hz, 2H), 3.99 (t, J=13.4 Hz, 2H).
2,2-difluoro-2-phenylethanol (371 mg, 73% yield) was prepared from methyl 2-fluoro-2-phenylacetate (commercially available) following a procedure analogous to step AB2. 1H NMR (500 MHz, chloroform-d) δ 7.45-7.24 (m, 1H), 5.65-5.51 (m, 1H), 4.01-3.78 (m, 3H), 2.26 (dd, J=8.4, 4.6 Hz, 1H).
2-Chloro-4-(2-fluoro-2-phenylethoxy)-3-(trifluoromethyl)pyridine (174 mg, 78% yield) was prepared from 2-fluoro-2-phenylethanol (from preparation AAL) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. 1H NMR (500 MHz, chloroform-d) δ 8.37 (d, J=5.8 Hz, 1H), 7.50-7.39 (m, 6H), 6.92-6.85 (m, 1H), 5.95-5.81 (m, 1H), 4.47 (ddd, J=16.9, 10.7, 7.3 Hz, 1H), 4.39-4.25 (m, 1H).
4-(2-Fluoro-2-phenylethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (172 mg, quantitative yield) was prepared from 2-chloro-4-(2-fluoro-2-phenylethoxy)-3-(trifluoromethyl)pyridine (from step AAM1) following a procedure analogous to step A2. 1H NMR (500 MHz, Methanol-d4) δ 8.18 (d, J=6.0 Hz, 1H), 7.50-7.37 (m, 5H), 6.58-6.53 (m, 1H), 5.90-5.75 (m, 1H), 4.52-4.34 (m, 2H).
1M Aqueous hydrochloric acid (7.5 mL, 7.50 mmol) was added to a solution of 8-(4-fluorophenyl)-1,4-dioxaspiro[4.5]decan-8-ol (2.0 g, 7.93 mmol, J. Am. Chem. Soc. 2012, 134, 17023) in acetone (20 mL). The mixture was left to stir at rt for 24 h. The reaction mixture was carefully quenched with aqueous sodium bicarbonate solution. The mixture was concentrated in vacuo to remove the acetone. The aqueous concentrate was extracted with ethyl acetate. The combined organic layers were washed with brine, dried over magnesium sulfate, filter and concentrated in vacuo. The residue was dissolved in minimal dichloromethane and loaded onto a prepacked silica cartridge (80 g). The crude product was purified using silica gel column chromatography (2:1 hexanes/ethyl acetate) to afford 4-(4-fluorophenyl)-4-hydroxycyclohexanone (1.3 g, 6.24 mmol, 79% yield) as a white solid. 1H NMR (500 MHz, CHLOROFORM-d) δ 7.55-7.46 (m, 2H), 7.11-7.03 (m, 2H), 2.92 (td, J=14.0, 6.4 Hz, 2H), 2.43-2.34 (m, 2H), 2.34-2.23 (m, 2H), 2.23-2.13 (m, 2H).
Sodium borohydride (0.590 g, 15.61 mmol) was added to a stirred solution of 4-(4-fluorophenyl)-4-hydroxycyclohexanone (1.3 g, 6.24 mmol) in ethanol (50 mL) maintained in a 0° C. ice bath. After 2 h, the reaction was diluted with water and the pH was adjusted to 2 by the addition of 1 N aqueous hydrochloric acid. The mixture was poured into water and extracted with ethyl acetate. The combined organics were washed with brine, dried (magnesium sulfate), filtered and concentrated in vacuo to afford (1s,4s)-1-(4-fluorophenyl)cyclohexane-1,4-diol (1.18 g, 5.61 mmol, 90% yield). 1H NMR integration was consistent with the desired product as a >10:1 ratio of diasteromers (cis/trans). Data for cis isomer: 1H NMR (500 MHz, DMSO-d6) δ 7.53-7.45 (m, 2H), 7.12-7.05 (m, 2H), 4.76 (s, 1H), 4.47 (d, J=4.6 Hz, 1H), 3.47 (tq, J=9.6, 4.7 Hz, 1H), 1.78-1.55 (m, 8H).
(1s,4s)-4-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(4-fluorophenyl)cyclohexanol (1.21 g, 62% yield) was prepared from (1s,4s)-1-(4-fluorophenyl)cyclohexane-1,4-diol (from preparation AAN) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. The crude product was purified using silica gel column chromatography (hexanes/ethyl acetate, 3:1-1:1). LC-MS (M+H)+ 390.2. 1H NMR (500 MHz, DMSO-d6) δ 8.46 (d, J=6.0 Hz, 1H), 7.57-7.45 (m, 3H), 7.22-7.09 (m, 2H), 5.05 (s, 1H), 4.89-4.74 (m, 1H), 2.00-1.88 (m, 6H), 1.78-1.67 (m, 2H).
(1s,4s)-1-(4-Fluorophenyl)-4-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)cyclohexanol (255 mg, 96% yield) was prepared from (1s,4s)-4-(2-chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(4-fluorophenyl)cyclohexanol (from step AAO1) following a procedure analogous to step A2. LC-MS (M+H)+ 386.2.
Sodium borohydride (0.253 g, 6.70 mmol) was added to a stirred solution of 3-(4-fluorophenyl)cyclobutanone (1.0 g, 6.09 mmol) in ethanol (40 mL) maintained in a 0° C. ice bath. After 2 h, the reaction was diluted with water and the pH was adjusted to 2 by the addition of 1 N aqueous hydrochloric acid. The mixture was extracted with ethyl acetate. The combined organics were washed with brine, dried (magnesium sulfate), filtered and concentrated in vacuo to afford (1r,3r)-3-(4-fluorophenyl)cyclobutanol (1.12 g, quantitative yield). The product was used without purification. 1H NMR integration suggested a 6:1 mixture of diastereomers (trans/cis). Data for trans isomer: 1H NMR (500 MHz, CHLOROFORM-d) δ 7.21-7.17 (m, 2H), 7.02-6.98 (m, 2H), 4.34-4.27 (m, 1H), 3.00-2.91 (m, 1H), 2.82-2.75 (m, 2H), 2.07 (d, J=2.0 Hz, 1H), 2.02-1.97 (m, 2H).
2-Chloro-4-((1r,3r)-3-(4-fluorophenyl)cyclobutoxy)-3-(trifluoromethyl)pyridine (1.22 g, 59% yield) was prepared from (1r,3r)-3-(4-fluorophenyl)cyclobutanol (from preparation AAP) and 2,4-dichloro-3-(trifluoromethyl)pyridine (WO 2010/130422) following a procedure analogous to step A1. LC-MS (M+H)+=346.1. 1H NMR (500 MHz, chlforoform-d) δ 8.35 (d, J=5.8 Hz, 1H), 7.24-7.19 (m, 2H), 7.07-7.01 (m, 2H), 6.79 (d, J=5.8 Hz, 1H), 4.80 (t, J=7.1 Hz, 1H), 3.28-3.19 (m, 1H), 3.04-3.00 (m, 2H), 2.39-2.32 (m, 2H).
4-((1r,3r)-3-(4-Fluorophenyl)cyclobutoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (486 mg, quantitative yield) was prepared from 2-chloro-4-((1r,3r)-3-(4-fluorophenyl)cyclobutoxy)-3-(trifluoromethyl)pyridine (from step AAQ1) following a procedure analogous to step A2. LC-MS (M+H)+ 345.1.
A solution of (1s,4s)-4-(2-Chloro-3-(trifluoromethyl)pyridin-4-yloxy)-1-(4-fluorophenyl)cyclohexanol (1s,4s)-4-((2-chloro-3-(trifluoromethyl)pyridin-4-yl)oxy)-1-(4-fluorophenyl)cyclohexanol (250 mg, 0.641 mmol, from step AAO1) in DMF (2 mL) was added to a stirred mixture of sodium hydride 60% dispersion in mineral oil (51.3 mg, 1.283 mmol) and DMF (5 mL) maintained at 0° C. After 30 min, a solution of iodomethane-d3 (232 mg, 1.604 mmol) in DMF (1 mL) was added. The resulting mixture was allowed to stir for 2 h at 0° C. The crude reaction was carefully quenched was water. The mixture was poured into water and extracted with ethyl acetate. The combined organics were washed with brine, dried over magnesium sulfate, filtered and concentrated in vacuo to afford 2-chloro-4-(((1s,4s)-4-(4-fluorophenyl)-4-(methoxy-d3)cyclohexyl)oxy)-3-(trifluoromethyl)pyridine. No purification was conducted. LC-MS (M+H)+ 407.2.
A mixture of 4-(((1s,4s)-4-(4-fluorophenyl)-4-(methoxy-d3)cyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (261 mg, 0.641 mmol), dioxane (50 mL), and hydrazine monohydrate (0.243 mL, 3.21 mmol) was heated together in a sealed vial in an oil bath at 95° C. for 12 h. The reaction mixture was concentrated in vacuo. The residue was partitioned between aqueous sodium bicarbonate and ethyl acetate. The aqueous layer was extracted with ethyl acetate. The combined organic extracts were washed with brine, dried over magnesium sulfate, filtered, and concentrated in vacuo to afford 4-(((1s,4s)-4-(4-fluorophenyl)-4-(methoxy-d3)cyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (275 mg, quantitative % yield). LC-MS (M+H)+ 403.2.
A 1.0 M solution of 2-cyclopropylacetyl chloride in DCM (0.55 mL, 0.55 mmol) was added to a flask charged with a solution of 2-hydrazinyl-4-((1-phenylcyclohexyl)methoxy)-3-(trifluoromethyl)pyridine (from preparation A, 200 mg, 0.547 mmol) and triethylamine (0.114 mL, 0.821 mmol) in DCM (5 mL) at 0° C. After 1 h at 0° C., the reaction was diluted with water. The aqueous mixture was extracted with DCM. The organic extracts were washed with brine, dried (sodium sulfate), filtered, and concentrated in vacuo to afford 2-cyclopropyl-N′-(4-((1-phenylcyclohexyl)methoxy)-3-(trifluoromethyl)pyridin-2-yl)acetohydrazide (245 mg, quantitative yield). The crude product was carried forward without purification. LC-MS (M+H)+ 480.0.
A mixture of 2-cyclopropyl-N′-(4-((1-phenylcyclohexyl)methoxy)-3-(trifluoromethyl)pyridin-2-yl)acetohydrazide (from step 1A, 245 mg, 0.547 mmol), Burgess reagent (130 mg, 0.547 mmol), acetonitrile (2 mL), and dioxane (2 mL) was heated at 85° C. in a sealed vial for 18 h. After cooling to rt, the reaction mixture was evaporated in vacuo and the residue was partitioned between water and ethyl acetate.
The aqueous layer was extracted with ethyl acetate, and the combined organic layers were washed with brine, dried over magnesium sulfate, filtered and concentrated in vacuo. The crude product was dissolved in a minimum amount of boiling ethyl acetate. The solution was allowed to cool to rt. After 3 h, the crystalline solid was collected by vacuum filtration to afford 3-(cyclopropylmethyl)-7-((1-phenylcyclohexyl)methoxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (107 mg, 45% yield) as a white solid.
The titled compound (157 mg, 65% yield over 2 steps) was prepared from 4-(2-(4-chlorophenyl)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation B, 200 mg, 0.556 mmol) following procedures analogous to steps 1A and 1B. The final product was purified by silica gel column chromatography (100% EtOAc).
N′-(3-cyano-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)pyridin-2-yl)-2-cyclopropylacetohydrazide (111 mg, quantitative yield) was prepared from 4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-2-hydrazinylnicotinonitrile (from preparation D, 87 mg, 0.292 mmol) following a procedure analogous to step 1A. The crude product was carried forward without purification. LC-MS (M+Na)+ 381.2.
A mixture of N′-(3-cyano-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)pyridin-2-yl)-2-cyclopropylacetohydrazide (from step 3A, 111 mg, 0.292 mmol), Burgess reagent (174 mg, 0.730 mmol), acetonitrile (2 mL), and dioxane (2 mL) was heated in a sealed vial in an oil bath at 110° C. for 4 h. The mixture was cooled to rt. The resulting precipitate was collected using vacuum filtration. The solid was rinsed with fresh dioxane (5 mL). The white solid was dried under vacuum to afford 3-(cyclopropylmethyl)-7-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-[1,2,4]triazolo[4,3-a]pyridine-8-carbonitrile (48 mg, 45% yield).
A solution of 2-(4-fluorophenyl)propan-1-ol (from preparation E, 69.9 mg, 0.453 mmol) in toluene (0.5 mL) was added to a microwave vial charged with 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine [(from J. Med Chem. 2012, 55, 8770), 25 mg, 0.091 mmol], cesium carbonate (59.1 mg, 0.181 mmol), (S)—(R)-JOSIPHOS (11 mg, 0.018 mmol), and allylpalladium chloride dimer (3.3 mg, 9.1 μmol). The vessel was flushed with nitrogen then sealed with a teflon coated cap. The mixture was heated at 135° C. for 2 h. The reaction was cooled to rt. The crude reaction mixture was purified by silica gel column chromatography (100% EtOAc) and was further purified using reverse phase preparatory HPLC.
The titled compound (240 mg, 69% yield over 2 steps) was prepared from 4-(2-(2-chloro-6-fluorophenyl)propoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation G) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (13 mg, 13% yield) was prepared from 2-(4-fluorophenyl)-2-methylpropan-1-ol (preparation H) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following procedures analogous to example 4. The final product was purified using preparative LC/MS (column: XBridge C18, 19×mm, 5-μm particles; mobile phase A: 5:95 acetonitrile/water with 10-mM ammonium acetate; mobile phase B: 95:5 acetonitrile/water with 10-mM ammonium acetate; gradient: 30-70% B over 15 minutes, then a 7-minute hold at 100% B; flow: 20 mL/min).
The titled compound (48 mg, 17% yield) was prepared from 4-(2-(4-chlorophenyl)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation B) and 3,3,3-trifluoropropanoyl chloride (commercially available) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (1:1 hexane/ethyl acetate) and was recrystallized from ethyl acetate/hexanes.
The titled compound (220 mg, 76% yield over 2 steps) was prepared from 4-(2-(4-fluorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation J) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
A racemic sample of 7-(2-(2-chloro-6-fluorophenyl)propoxy)-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from example 5, 52 mg) was purified using chiral preparatory HPLC to afford 14.4 mg of example 9 (first to elute) and 18.8 mg of example 10 (second to elute). HPLC Method: Chiralcel OD (21×250 mm, 10 uM), 20% ethanol/80% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined.
The titled compound (56 mg, 19% yield over 2 steps) was prepared from 4-(2-(4-fluorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation J) and 3,3,3-trifluoropropanoyl chloride (commercially available) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc) and was recrystallized from ethyl acetate/hexane.
The titled compound (105 mg, 27% yield over 2 steps) was prepared from 2-(4-fluorophenyl)-3-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)-2-methylpropanenitrile (from preparation L) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc) and reverse phase preparatory HPLC.
The titled compound (173 mg, 56% yield over 2 steps) was prepared from 4-(2-(4-chlorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation N) following procedures analogous to steps 1A and 1B. The product was purified using silica gel column chromatography (100% EtOAc) and was recrystallized from ethyl acetate/hexanes.
A racemic sample of 3-((3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)oxy)-2-(4-fluorophenyl)-2-methylpropanenitrile, TFA (105 mg, 27% yield) (from example 12, 53 mg) was purified using chiral preparatory HPLC to afford 18.5 mg of example 14 (first to elute) and 16.7 mg of example 15 (second to elute). HPLC Method: ChiralPak AS-H (21×250 mm, 10 uM), 20% ethanol/80% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined.
The titled compound (173 mg, 54% yield over 2 steps) was prepared from 4-(2-(2,4-difluorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation P) following procedures analogous to steps 1A and 1B. The product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (154 mg, 51% yield over 2 steps) was prepared from (1s,4s)-1-(4-fluorophenyl)-4-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)cyclohexanecarbonitrile (from preparation R) following procedures analogous to steps 1A and 1B. The product was purified using silica gel column chromatography (ISCO system, 100% EtOAc).
N′-(4-(2-(4-Chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridin-2-yl)-2-ethoxyacetohydrazide (153 mg, 83% yield) was prepared from 4-(2-(4-chlorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation N) and 2-ethoxyacetyl chloride (commercially available) following a procedure analogous to step 1A. LC-MS (M+H)+ 462.1.
The titled compound (40 mg, 27% yield over 2 steps) was prepared from N′-(4-(2-(4-chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridin-2-yl)-2-ethoxyacetohydrazide (from step 18A) following a procedure analogous to step 1B. The crude product was purified using silica gel column chromatography (100% EtOAc) and then preparative LC/MS (column: XBridge C18, 19×200 mm, 5-μm particles; mobile phase A: 5:95 acetonitrile:water with 10-mM ammonium acetate; mobile phase B: 95:5 acetonitrile:water with 10-mM ammonium acetate; gradient: 30-80% B over 40 minutes, then a 5-minute hold at 100% B; flow: 20 mL/min).
The titled compound (92 mg, 67% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation S) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (147 mg, 64% yield over 2 steps) was prepared from 2-hydrazinyl-4-(((1s,4s)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridine (from preparation T) following procedures analogous to steps 1A and 1B. The product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (131 mg, 56% yield over 2 steps) was prepared from 4-(2-(4-fluorophenyl)-2-methoxypropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation V) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
N′-(4-(2-(4-Chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridin-2-yl)-2-(2,2,2-trifluoroethoxy)acetohydrazide (130 mg, 100% yield) was prepared from 4-(2-(4-chlorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation N) and 2-(2,2,2-trifluoroethoxy)acetyl chloride (WO 87/07270) following a procedure analogous to step 1A. LC-MS (M+H)+ 516.1.
The titled compound (39 mg, 25% yield over 2 steps) was prepared from N′-(4-(2-(4-chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridin-2-yl)-2-(2,2,2-trifluoroethoxy)acetohydrazide (from step 22A) following a procedure analogous to step 1B. The crude product was purified using silica gel column chromatography (20% EtOAc/hexanes) and then reverse phase preparatory HPLC.
N′ N′-(4-(2-(4-Chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridin-2-yl)-2-methoxyacetohydrazide (113 mg, 100% yield) was prepared from 4-(2-(4-chlorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation N) and 2-methoxyacetyl chloride (commercially available) following a procedure analogous to step 1A. LC-MS (M+H)+ 448.1.
The titled compound (43 mg, 30% yield over 2 steps) was prepared from N′ N′-(4-(2-(4-Chlorophenoxy)-2-methylpropoxy)-3-(trifluoromethyl)pyridin-2-yl)-2-methoxyacetohydrazide (from step 23A) following a procedure analogous to step 1B. The crude product was purified using silica gel column chromatography (100% EtOAc) and then reverse phase preparatory HPLC.
A sample of 3-(cyclopropylmethyl)-7-(2-(4-fluorophenyl)-2-methoxypropoxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine, TFA (example 21, 50 mg) was purified using chiral preparatory HPLC to afford 20 mg of example 24 (first to elute) and 19 mg of example 25 (second to elute). HPLC Method: ChiralCel OD (21×250 mm, 10 uM), 20% ethanol/80% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined.
The titled compound (4.8 mg, 16% yield) was prepared from (1s,4s)-1-(3,4-difluorophenyl)-4-((2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yl)oxy)cyclohexanol (from preparation X) following procedures analogous to steps 1A and 1B. The crude product mixture was purified using reverse phase preparatory HPLC.
N′-(4-(((1r,4r)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridin-2-yl)-2-(2,2,2-trifluoroethoxy)acetohydrazide (266 mg, 0.541 mmol, quantitative yield) was prepared from 2-hydrazinyl-4-(((1s,4s)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridine (from preparation T) and 2-(2,2,2-trifluoroethoxy)acetyl chloride (WO 87/07270) following a procedure analogous to step 1A. LC-MS (M+H)+ 492.2.
The titled compound (141 mg, 54% yield over two steps) was prepared from N′-(4-(((1r,4r)-4-phenylcyclohexyl)oxy)-3-(trifluoromethyl)pyridin-2-yl)-2-(2,2,2-trifluoroethoxy)acetohydrazide (from step 27A) following a procedure analogous to step 1B. The crude product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (49 mg, 22% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(3,4-difluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation Y) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (131 mg, 20% yield over 2 steps) was prepared from 4-((1-(4-chlorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation Z) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (100% ethyl acetate).
The titled compound (64 mg, 9% yield over 2 steps) was prepared from 4-((1-(4-fluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AA) following the procedures detailed in steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate/hexanes).
The titled compound (145 mg, 26% yield over 2 steps) was prepared from 4-((2-(4-chlorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AC) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate/hexanes).
The titled compound (43 mg, 11% yield over 2 steps) was prepared from 4-((2-(2-fluoro-6-methoxyphenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AE) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (100% ethyl acetate).
A sample of (±)-7-(((1S,2S)-2-(4-chlorophenyl)cyclopropyl)methoxy)-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from example 31, 233 mg) was purified using chiral preparatory HPLC to afford 61 mg of example 33 (first enantiomer to elute) and 60 mg of example 34 (second enantiomer to elute). HPLC Method: ChiralCel OD (21×250 mm, 10 uM), 10% ethanol/90% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 254 nm. The absolute stereochemistry of example 34 was confirmed as (1S,2S) through an independent asymmetric synthesis. The stereochemistry of example 33 was assigned as (1R,2R) by default.
A sample of (±)-3-(cyclopropylmethyl)-7-(((1S,2S)-2-(2-fluoro-6-methoxyphenyl)cyclopropyl)methoxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (example 32, 39 mg) was purified using chiral preparatory HPLC to afford 11 mg of example 35 (first to elute) and 10 mg of example 36 (second to elute). HPLC Method: Chiralcel OD (21×250 mm, 10 uM), 20% ethanol/80% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of the individual enantiomers was not determined.
The titled compound (61 mg, 40% yield over 2 steps) was prepared from (±)-2-hydrazinyl-4-(((1S,2S)-2-(2-methoxyphenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from preparation AG) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (100% ethyl acetate).
The titled compound (71 mg, 43% yield over 2 steps) was prepared from (±)-2-hydrazinyl-4-(((1S,2S)-2-(2-(trifluoromethoxy)phenyl)cyclopropyl)methoxy)-3-(trifluoromethyl)pyridine (from preparation AI) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate).
A sample of (±)-3-(cyclopropylmethyl)-7-(((1S,2S)-2-(2-methoxyphenyl)cyclopropyl)methoxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (example 37, 50 mg) was purified using chiral preparatory HPLC to afford 11 mg of example 39 (first to elute) and 11 mg of example 40 (second to elute). HPLC Method: Chiralcel OD-H (21×250 mm, 10 uM), 20% ethanol/80% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined.
A sample of (±)-3-(cyclopropylmethyl)-7-(((1S,2S)-2-(2-(trifluoromethoxy)phenyl)cyclopropyl)methoxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (example 38, 61 mg) was purified using chiral supercritical fluid chromatography (SFC) to afford 20 mg of example 41 (first to elute) and 22 mg of example 42 (second to elute). SFC Method: Chiralcel OD-H (30×250 mm, 5 uM), 15% methanol (0.1% DEA)/90% CO2, 150 bar, 70 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined.
The titled compound (1.4 mg, 2% yield) was prepared from (±)-((1S,2S)-2-(2-fluorophenyl)cyclopropyl)methanol (from preparation AJ) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedures and purification methods as described for example 4.
The titled compound (6.4 mg, 8% yield) was prepared from (±)-((1S,2S)-2-(2,5-difluorophenyl)cyclopropyl)methanol (from preparation AK) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedure and purification method as described for example 4.
The titled compound (4.5 mg, 6% yield) was prepared from (±)-((1S,2S)-2-(4-methoxyphenyl)cyclopropyl)methanol (from preparation AL) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedures and purification methods as described for example 4.
The titled compound (345 mg, 21% yield over 2 steps) was prepared from (1s,3s)-1-(3-fluorophenyl)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclobutanecarbonitrile (from preparation AM) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate).
The titled compound (9.1 mg, 11% yield) was prepared from (±)-((1S,2S)-2-(3,6-difluoro-2-methoxyphenyl)cyclopropyl)methanol (from preparation AN) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedures and purification method as described for example 4.
The titled compound (4.5 mg, 5% yield) was prepared from (±)-((1S,2S)-2-(2-chloro-6-fluorophenyl)cyclopropyl)methanol (from preparation AO) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedures and purification methods as described for example 4.
The titled compound (2.3 mg, 3% yield) was prepared from (±)-((1S,2S)-2-(4-fluoro-2-methoxyphenyl)cyclopropyl)methanol (from preparation A2) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedures and purification methods as described for example 4.
The titled compound (235 mg, 42% yield over 2 steps) was prepared from (1s,3s)-1-(4-fluorophenyl)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclobutanecarbonitrile (from preparation AQ) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate).
The titled compound (2.4 mg, 2% yield) was prepared from (1-(2,4-difluorophenyl)cyclopropyl)methanol (from preparation AR) and 7-chloro-3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (from J. Med Chem. 2012, 55, 8770) following the same procedures and purification methods as described for example 4.
The titled compound (232 mg, 18% yield over 2 steps) was prepared from 4-(2-(4-fluorophenoxy)-2-methylpropoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AS) following procedures analogous to steps 7A and 1B. The final product was purified using silica gel column chromatography (0-100% EtOAc).
The titled compound (435 mg, 36% yield over 2 steps) was prepared from (1s,3s)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)-1-(2-methoxyphenyl)cyclobutanecarbonitrile (from preparation AU) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate).
The titled compound (20 mg, 4% yield over 2 steps) was prepared from (1s,3s)-1-(3,5-difluorophenyl)-3-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclobutanecarbonitrile (from preparation AW) following procedures analogous to steps 1A and 1B. The final product was purified by preparative LC-MS.
The titled compound (266 mg, 41% yield over 2 steps) was prepared from 4-((1-(3,5-difluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AY) following procedures analogous to steps 1A and 1B. The final product was purified by preparative LC-MS.
The titled compound (101 mg, 15% yield over 2 steps) was prepared from 4-((1-(3,5-dichlorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAA) following procedures analogous to steps 1A and 1B. The final product was purified by preparative LC-MS.
The titled compound (40 mg, 34% yield over 2 steps) was prepared from 2-hydrazinyl-4-((1r,3r)-3-phenylcyclobutoxy)-3-(trifluoromethyl)pyridine (from preparation AAC) following procedures analogous to steps 1A and 1B. The final product was purified by preparative LC-MS.
A mixture of 1,10-phenanthroline (22.4 mg, 0.124 mmol), (4-chlorophenyl)methanol (commercially available, 108 mg, 0.757 mmol), 8-chloro-3-(cyclopropylmethyl)-7-iodo-[1,2,4]triazolo[4,3-a]pyridine ((from J. Med. Chem. 2012, 55, 8770), 35 mg, 0.105 mmol), copper(I) iodide (38 mg, 0.200 mmol), cesium carbonate (52 mg, 0.160 mmol), and toluene (1 mL) was heated in a sealed vial in an oil bath at 100° C. for 24 h. The reaction mixture was allowed to cool to rt and was filtered through celite. The solid was washed with acetonitrile. The filtrate was concentrated in vacuo. The residue was purified using reverse phase preparatory HPLC.
The titled compound (1.0 mg, 2% yield) was prepared from (4-chlorophenyl)methanol (commercially available) and 8-chloro-3-(cyclopropylmethyl)-7-iodo-[1,2,4]triazolo[4,3-a]pyridine (from J. Med. Chem. 2012, 55, 8770) following a procedure analogous to example 58. The product was purified using reverse phase preparatory HPLC.
Examples 60-76 were prepared in parallel fashion according to the following method. A stock solution of 8-chloro-3-(cyclopropylmethyl)-7-iodo-[1,2,4]triazolo[4,3-a]pyridine [(from J. Med. Chem. 2012, 55, 8770), 0.9 M in toluene, 1 mL, 0.090 mmol] was added to a 2.0-5.0 mL microwave vial charged with the corresponding commercially available alcohol (0.648 mmol), cesium carbonate (50.0 mg, 0.153 mmol), 1,10-phenanthroline (20.0 mg, 0.111 mmol), and 20.0 mg of copper(I) iodide (20.0 mg, 0.105 mmol). The vials were sealed and heated to 100° C. for 17 hours. The reaction mixtures were cooled to rt, diluted with 0.5 mL of acetonitrile, and then filtered. The filtrates were concentrated in vacuo at 34° C. for 1 hour. Each sample was diluted with 0.5 mL of DMF (0.5 mL) and purified using reverse phase preparative LC-MS.
To a stirred mixture of 3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-ol (from preparation AAD, 32 mg, 0.12 mmol), 2-(bromomethyl)-1,3-difluorobenzene (31 mg, 0.15 mmol), and tetrahydrofuran (4 mL) was added potassium tert-butoxide (31 mg, 0.28 mmol) followed by DMF (1.5 mL) and the reaction mixture was stirred at rt overnight. The reaction mixture was concentrated in vacuo. The residue was purified using reverse phase preparative HPLC to afford the titled compound (16 mg, 34% yield) as a white solid.
The titled compound (80 mg, 20% yield over 2 steps) was prepared from (±)-4-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAF) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate) and reverse phase preparatory HPLC.
A sample of (±)-3-(cyclopropylmethyl)-7-(((1S,2S)-2-(4-fluorophenyl)cyclopropyl)methoxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (example 78, 60 mg) was purified using chiral preparatory HPLC to afford 22 mg of example 79 (first enantiomer to elute) and 22 mg of example 80 (second enantiomer to elute). HPLC Method: Chiralcel OD-H (21×250 mm, 10 uM), 20% ethanol/80% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined.
The titled compound (43 mg, 8% yield over 2 steps) was prepared from 4-(2-chloro-6-fluorophenethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAG) following procedures analogous to steps 1A and 1B. The final product was purified by silica-gel column chromatography (0-100% ethyl acetate) and reverse phase preparatory HPLC.
The titled compound (67 mg, 16% yield over 2 steps) was prepared from 4-(2,6-difluorophenethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAI) following procedures analogous to steps 1A and 1B. The final product was purified by reverse phase preparatory HPLC. The resulting TFA salt was dissolved in a minimum of methanol and partioned between ethyl acetate and aqueous sodium carbonate. The organic layer was washed with brine, dried over magnesium sulfate, filtered, and concentrated in vacuo to afford the titled compound.
The titled compound (6.6 mg, 5% yield) was prepared from 3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-ol (from preparation AAD) and 2,4-dichloro-6-(chloromethyl)pyrimidine (commercially available) following a procedure analogous to that described in example 76.
The titled compound (59 mg, 17% yield over 2 steps) was prepared from 4-(2,2-difluoro-2-phenylethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAK) following procedures analogous to steps 1A and 1B. The final product was purified by silica gel column chromatography (100% ethyl acetate) and reverse phase preparatory HPLC.
The titled compound (69 mg, 18% yield over 2 steps) was prepared from 4-(2-fluoro-2-phenylethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAM) following procedures analogous to steps 1A and 1B. The final product was purified by silica gel column chromatography (100% ethyl acetate) and reverse phase preparatory HPLC.
The titled compound (60 mg, 28% yield over 2 steps) was prepared from 4-(2-fluoro-2-phenylethoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from J. Med. Chem. 2012, 55, 8770) following procedures analogous to steps 1A and 1B. The final product was purified by silica gel column chromatography (100% ethyl acetate).
A solution of N-ethyldiisopropylamine (0.424 mL, 2.429 mmol) in DMF (5 mL) was added to vessel charged with 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (194 mg, 0.486 mmol, from preparation S), 2,2-difluorocyclopropanecarboxylic acid (59.3 mg, 0.486 mmol, commercially available), and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (233 mg, 1.214 mmol). The flask containing the resulting mixture was stoppered and left to stir at room temperature for 18 hours. The crude reaction mixture was poured into water and extracted with EtOAc. Hexane (˜5% total volume) was added to the combined organics. The organic layer was washed with brine, dried over sodium sulfate, filtered, and concentrated in vacuo. The crude product was purified using silica gel column chromatography (100% EtOAc) to afford 2,2-difluoro-N′-(4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-3-(trifluoromethyl)pyridin-2-yl)cyclopropanecarbohydrazide (129 mg, 0.256 mmol, 53% yield) off-white foam solid. LC-MS (M+H)+ 504.2.
A mixture of 2,2-difluoro-N′-(4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-3-(trifluoromethyl)pyridin-2-yl)cyclopropanecarbohydrazide (129 mg, 0.256 mmol), Burgess reagent (130 mg, 0.547 mmol), acetonitrile (2 mL), and dioxane (2 mL) was heated at 85° C. in a sealed vial for 18 h. After cooling to rt, the reaction mixture was evaporated in vacuo and the residue was partitioned between water and ethyl acetate. The aqueous layer was extracted with ethyl acetate, and the combined organic layers were washed with brine, dried over magnesium sulfate, filtered and concentrated in vacuo. The crude product was purified using silica gel column chromatography (100% EtOAc) to afford 3-(2,2-difluorocyclopropyl)-7-((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyloxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (108 mg, 85% yield) as a white solid.
A sample of (±)-3-(2,2-difluorocyclopropyl)-7-((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyloxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (example 86, 61 mg) was purified using chiral HPLC to afford the separated enantiomers. Each enantiomer was separately repurified using reverse phase preparatory HPLC to afford 21 mg of example 87 (TFA salt, first to elute from chiral column) and 16 mg of example 87 (TFA salt, second to elute from chiral column). Chiral HPLC Method: ChiralPak OD (21×250 mm, 10 uM), 10% ethanol/90% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of the individual enantiomers was not determined.
The titled compound (136 mg, 46% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation S) and commercially available trans-2-fluorocyclopropanecarboxylic acid following procedures analogous to steps 86A and 86B. The final product was purified using silica gel column chromatography (100% EtOAc).
A sample of (±)-rel-3-((1S,2R)-2-fluorocyclopropyl)-7-((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyloxy)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridine (example 89, 60 mg) was purified using chiral HPLC to afford to afford 14 mg of example 90 (first to elute) and 17 mg of example 91 (second to elute from chiral column). Chiral HPLC Method: ChiralPak AS (21×250 mm, 10 uM), 10% ethanol/90% heptane (with 0.1% diethylamine), 15 mL/min, absorbance 220 nm. The absolute stereochemistry of individual enantiomers was not determined. The absolute stereochemistry of the individual enantiomers was not determined.
The titled compound (37 mg, 33% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation S) and commercially available cyclopropanecarboxylic acid following procedures analogous to steps 86A and 86B. The final product was purified using preparative LC-MS under the standard conditions.
The titled compound (57 mg, 44% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation S) and commercially available 3,3-difluorocyclobutanecarboxylic acid following procedures analogous to steps 86A and 86B. The final product was purified using preparative LC-MS under the standard conditions.
The titled compound (16 mg, 18% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation S) and commercially available 2,2-dimethylcyclopropanecarboxylic acid following procedures analogous to steps 86A and 86B. The final product was purified using preparative LC-MS under the standard conditions.
The titled compound (16 mg, 4% yield over 2 steps) was prepared from (1s,4s)-1-(4-fluorophenyl)-4-(2-hydrazinyl-3-(trifluoromethyl)pyridin-4-yloxy)cyclohexanol (from preparation AAO) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc) and further purified using reverse phase preparatory HPLC under the standard conditions.
The titled compound (103 mg, 38% yield over 2 steps) was prepared from 4-((1r,3r)-3-(4-fluorophenyl)cyclobutoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAQ) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (28 mg, 22% yield over 2 steps) was prepared from 4-(((1s,4s)-4-(4-fluorophenyl)-4-methoxycyclohexyl)oxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation S) and 3,3,3-trifluoropropanoyl chloride (commercially available) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (24 mg, 34% yield over 2 steps) was prepared from 4-((1 r,3r)-3-(4-fluorophenyl)cyclobutoxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAQ) and commercially available 2,2-difluorocyclopropanecarboxylic acid following procedures analogous to steps 86A and 86B. The final product was purified using silica gel column chromatography (100% EtOAc).
The titled compound (59 mg, 18% yield over 2 steps) was prepared from 4-((1s,4s)-4-(4-fluorophenyl)-4-(methoxy-d3)cyclohexyloxy)-2-hydrazinyl-3-(trifluoromethyl)pyridine (from preparation AAR) following procedures analogous to steps 1A and 1B. The final product was purified using silica gel column chromatography (100% EtOAc) and further purified using reverse phase preparatory HPLC under the standard conditions. The resulting TFA salt was free based by dissolving the purified product in aqueous sodium bicarbonate solution, extracting with ethyl acetate, and reconcentrating the organic extract.
To a solution of (1s,3s)-3-((3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)oxy)-1-(2-methoxyphenyl)cyclobutanecarbonitrile (10 mg, 0.023 mmol, example 53) in acetonitrile (1.0 mL) was added neat bromine (0.058 mL, 1.13 mmol). The mixture was stirred at 45° C. for 12 h. The resulting mixture was concentrated in vacuo and purified using preparatory LC-MS under the standard conditions to afford (1s,3s)-3-(3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-yloxy)-1-(3,5-dibromo-2-methoxyphenyl)cyclobutanecarbonitrile (7.5 mg, 51% yield). LC-MS (M+H)+ 601.1. 1H NMR (500 MHz, DMSO-d6) δ 8.66 (d, J=7.6 Hz, 1H), 8.01-7.94 (m, 1H), 7.71 (s, 1H), 6.95 (d, J=7.9 Hz, 1H), 5.20-5.03 (m, 1H), 3.95 (s, 3H), 3.08-2.97 (m, 4H), 1.23-1.11 (m, 1H), 0.56-0.45 (m, 2H), 0.27 (d, J=4.6 Hz, 2H).
A solution of (1s,3s)-3-(3-(cyclopropylmethyl)-8-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]pyridin-7-yloxy)-1-(3,5-dibromo-2-methoxyphenyl)cyclobutanecarbonitrile (1.4 mg, 2.57 μmol, from step 100A) and triethylamine (0.040 ml) in 0.5 ml ethanol was added to 10% palladium on carbon (4.0 mg). The mixture was exposed to 1 Ci of tritium gas and vigorously stirred at ambient temperature for 4.2 h. The catalyst was removed by filtration and the crude product was twice concentrated by rotary evaporation with added ethanol (3×2 ml). The residue was dissolved in 5 ml of ethanol. The solution of the crude product was assayed by scintillation counting and was found to contain 58 mCi radioactivity. The crude product was purified using preparatory HPLC (column: LUNA 5μ, C18(2) (10×250 mm); solvent A: CH3CN/H2O=40/60(0.1% TFA); solvent B: CH3CN; lambda=224 nm; method: 0-5 min 0% B@1 ml/min; 5-6 min 0% B@1-4.5 ml/min; 6-30 min 0% B 4.5 ml/min; 30-35 min 0-100% B at 4.5 ml/min) to afford a sample of the titled compound having 93.7% radiochemical purity. The sample was repurified by preparatory HPLC (column: LUNA 5u C18(2)(10×250 mm); solvent A: CH3CN/H2O=30/70 (0.1% TFA); solvent B: CH3CN; lambda=224 nm; 0-5 min 0% B@1 ml/min; 5-6 min 0% B@1-4.5 ml/min; 6-66 min 0% B@4.5 ml/min; 66-70 min 0-100% B at 4.5 ml/min) to afford a sample of the titled compound having 99.5% radiochemical purity. S.A.=38.0 Ci/mmol. MS m/z (441.00-451.92) average=446.08.
1H NMR data
1H NMR (500 MHz, DMSO-d6) δ 8.62 (d, J = 7.8 Hz, 1H), 7.48 (d, J = 7.5
1H NMR (500 MHz, chloroform-d) δ 8.06 (d, J = 7.6 Hz, 1H), 7.46-7.36
1H NMR (500 MHz, methanol-d4) δ 8.59 (d, J = 7.8 Hz, 1H), 7.22-7.14
1H NMR (500 MHz, methanol-d4) δ 8.52 (d, J = 7.8 Hz, 1H), 7.44-7.28
1H NMR (500 MHz, chloroform-d) δ 8.14 (d, J = 7.6 Hz, 1H), 7.25-7.14
1H NMR (500 MHz, DMSO-d6) δ 8.66 (d, J = 7.6 Hz, 1H), 7.50 (dd, J = 8.5,
1H NMR (500 MHz, chloroform-d) δ 8.08 (d, J = 7.8 Hz, 1H), 7.42-7.30
1H NMR (500 MHz, chloroform-d) δ 8.15 (d, J = 7.6 Hz, 1H), 7.02-6.93
1H NMR (500 MHz, DMSO-d6) δ 8.68 (d, J = 7.3 Hz, 1H), 7.37-7.28 (m,
1H NMR data identical to example 9.
1H NMR (500 MHz, chloroform-d) δ 8.16 (d, J = 7.8 Hz, 1H), 7.03-6.88
1H NMR (500 MHz, chloroform-d) δ 8.50 (d, J = 6.6 Hz, 1H), 7.55 (dd,
1H NMR (500 MHz, chloroform-d) δ 8.14 (br. s., 1H), 7.26-7.22 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.74 (d, J = 7.6 Hz, 1H), 7.73-7.62 (m,
1H NMR data identical to example 14.
1H NMR (500 MHz, chloroform-d) δ 8.13 (d, J = 7.5 Hz, 1H), 7.05 (td,
1H NMR (500 MHz, chloroform-d) δ 8.21 (d, J = 7.6 Hz, 1H), 7.54-7.45
1H NMR (500 MHz, DMSO-d6) δ 8.67 (d, J = 7.6 Hz, 1H), 7.40-7.27 (m,
1H NMR (500 MHz, chloroform-d) δ 8.06 (d, J = 7.8 Hz, 1H), 7.38 (dd,
1H NMR (500 MHz, chloroform-d) δ 8.07 (d, J = 7.6 Hz, 1H), 7.39-7.29
1H NMR (500 MHz, chloroform-d) δ 8.02 (d, J = 7.8 Hz, 1H), 7.51-7.40
1H NMR (500 MHz, methanol-d4) δ 8.69 (d, J = 7.8 Hz, 1H), 7.43 (d, J = 7.8
1H NMR (500 MHz, methanol-d4) δ 8.81 (d, J = 7.8 Hz, 1H), 7.53 (d, J = 7.9
1H NMR (500 MHz, DMSO-d6) δ 8.66 (d, J = 7.6 Hz, 1H), 7.51 (d, J = 5.8
1H NMR data identical to example 24.
1H NMR (500 MHz, methanol-d4) δ 8.89 (d, J = 7.9 Hz, 1H), 7.71 (d, J = 7.8
1H NMR (500 MHz, methanol-d4) δ 8.59 (d, J = 7.8 Hz, 1H), 7.38 (d, J = 7.8
1H NMR (500 MHz, methanol-d4) δ 8.53 (d, J = 7.8 Hz, 1H), 7.39-7.31
1H NMR (500 MHz, chloroform-d) δ 8.23 (d, J = 7.6 Hz, 1H), 7.46-7.40
1H NMR (500 MHz, chloroform-d) δ 8.38 (d, J = 7.6 Hz, 1H), 7.46-7.40
1H NMR (500 MHz, DMSO-d6) δ 8.70 (d, J = 7.8 Hz, 1H), 7.33-7.27 (m,
1H NMR (400 MHz, chloroform-d) δ 8.15 (d, J = 7.8 Hz, 1H), 7.12 (td,
1H NMR (500 MHz, DMSO-d6) δ 8.69 (d, J = 7.6 Hz, 1H), 7.35-7.25 (m,
1H NMR data identical to example 33.
1H NMR (500 MHz, DMSO-d6) δ 8.68 (d, J = 7.9 Hz, 1H), 7.22 (d, J = 7.9
1H NMR data identical to example 35.
1H NMR (500 MHz, DMSO-d6) δ 8.70 (d, J = 7.6 Hz, 1H), 7.24 (d, J = 7.6
1H NMR (500 MHz, chloroform-d) δ 8.11 (d, J = 7.8 Hz, 1H), 7.27-7.24
1H NMR (500 MHz, DMSO-d6) δ 8.70 (d, J = 7.6 Hz, 1H), 7.24 (d, J = 7.6
1H NMR data identical to example 39.
1H NMR (500 MHz, DMSO-d6) δ 8.71 (d, J = 7.6 Hz, 1H), 7.30 (s, 3H),
1H NMR data identical to example 41.
1H NMR (500 MHz, DMSO-d6) δ 8.70 (d, J = 7.6 Hz, 1H), 7.26-7.18 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.70 (d, J = 7.6 Hz, 1H), 7.26-7.13 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.70 (d, J = 7.6 Hz, 1H), 7.22 (d, J = 7.9
1H NMR (500 MHz, methanol-d4) δ 8.56 (d, J = 7.8 Hz, 1H), 7.54 (td,
1H NMR (500 MHz, DMSO-d6) δ 8.71 (d, J = 7.9 Hz, 1H), 7.24 (d, J = 7.9
1H NMR (500 MHz, methanol-d4) δ 8.88 (d, J = 7.8 Hz, 1H), 7.61 (d, J = 7.8
1H NMR (500 MHz, DMSO-d6) δ 8.69 (d, J = 7.6 Hz, 1H), 7.23 (d, J = 7.9
1H NMR (500 MHz, chloroform-d) δ 8.09 (d, J = 7.6 Hz, 1H), 7.55-7.45
1H NMR (500 MHz, DMSO-d6) δ 8.40 (d, J = 7.9 Hz, 1H), 7.20 (q, J = 8.0
1H NMR (500 MHz, methanol-d4) δ 8.68 (d, J = 7.9 Hz, 1H), 7.31 (d, J = 7.8
1H NMR (500 MHz, methanol-d4) δ 8.54 (d, J = 7.8 Hz, 1H), 7.49-7.40
1H NMR (500 MHz, DMSO-d6) δ 8.69 (d, J = 7.9 Hz, 1H), 7.42 (d, J = 6.7
1H NMR (600 MHz, DMSO-d6) δ 8.68 (d, J = 5.5 Hz, 1H), 7.12 (d, J = 7.7
1H NMR (600 MHz, DMSO-d6) δ 8.68 (d, J = 7.3 Hz, 1H), 7.44 (s, 1H),
1H NMR (500 MHz, DMSO-d6) δ 8.68 (d, J = 7.6 Hz, 1H), 7.38-7.14 (m,
1H NMR (500 MHz, methanol-d4) δ 8.67 (d, J = 7.6 Hz, 1H), 7.56 (d, J = 7.6
1H NMR (500 MHz, methanol-d4) δ 8.35 (d, J = 7.6 Hz, 1H), 7.51 (dd,
1H NMR (500 MHz, DMSO-d6) δ 8.51 (d, J = 7.6 Hz, 1H), 7.58 (s, 1H),
1H NMR (500 MHz, DMSO-d6) δ 8.48 (d, J = 7.6 Hz, 1H), 7.34 (t, J = 7.9
1H NMR (500 MHz, DMSO-d6) δ 8.50-8.47 (m, 2H), 8.43 (dd, J = 4.6, 1.5
1H NMR (500 MHz, DMSO-d6) δ 8.52 (d, J = 7.6 Hz, 1H), 7.32 (dd, J = 8.7,
1H NMR (500 MHz, DMSO-d6) δ 8.47 (d, J = 7.6 Hz, 1H), 7.41-7.31 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.49 (d, J = 7.6 Hz, 1H), 7.32 (dd, J = 8.5,
1H NMR (500 MHz, DMSO-d6) δ 8.53 (d, J = 7.6 Hz, 1H), 7.71-7.64 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.49 (d, J = 7.6 Hz, 1H), 7.37-7.13 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.53 (d, J = 7.6 Hz, 1H), 7.62 (t, J = 7.5
1H NMR (500 MHz, DMSO-d6) δ 8.51 (d, J = 7.6 Hz, 1H), 7.78 (d, J = 1.8
1H NMR (500 MHz, DMSO-d6) δ 8.48 (d, J = 7.6 Hz, 1H), 7.43 (d, J = 8.5
1H NMR (500 MHz, DMSO-d6) δ 8.54 (d, J = 7.3 Hz, 1H), 7.56 (t, J = 7.0
1H NMR (500 MHz, DMSO-d6) δ 8.51 (d, J = 7.3 Hz, 1H), 7.51-7.41 (m,
1H NMR (500 MHz, DMSO-d6) δ 8.52 (d, J = 5.8 Hz, 1H), 7.82 (br. s., 1H),
1H NMR (500 MHz, DMSO-d6) δ 8.47 (d, J = 7.6 Hz, 1H), 7.29 (d, J = 8.9
1H NMR (500 MHz, DMSO-d6) δ 8.44 (d, J = 7.3 Hz, 1H), 7.33 (t, J = 7.8
1H NMR (500 MHz, methanol-d4) δ 8.97 (d, J = 7.9 Hz, 1H), 7.78 (d, J = 7.9
1H NMR (500 MHz, methanol-d4) δ 8.56 (d, J = 7.8 Hz, 1H), 7.21 (d, J = 7.5
1H NMR (500 MHz, methanol-d4) δ 8.56 (d, J = 7.8 Hz, 1H), 7.21 (d, J = 7.6
1H NMR data was identical to example 79.
1H NMR (400 MHz, methanol-d4) δ 8.54 (d, J = 7.8 Hz, 1H), 7.31-7.24
1H NMR (500 MHz, methanol-d4) δ 8.55 (d, J = 7.8 Hz, 1H), 7.37-7.27
1H NMR (500 MHz, methanol-d4) δ 8.77-8.69 (m, 1H), 7.49 (s, 1H),
1H NMR (500 MHz, methanol-d4) δ 8.56 (d, J = 7.8 Hz, 1H), 7.65 (dd,
1H NMR (500 MHz, methanol-d4) δ 8.57-8.53 (m, 1H), 7.53-7.37 (m,
1H NMR (500 MHz, chloroform-d) δ 8.04 (d, J = 7.6 Hz, 1H), 7.53-7.31
1H NMR (500 MHz, methanol-d4) δ 8.58 (d, J = 7.8 Hz, 1H), 7.52-7.43
1H NMR (500 MHz, methanol-d4) δ 8.82 (d, J = 7.8 Hz, 1H), 7.57 (d,
1H NMR data was identical to example 87.
1H NMR (500 MHz, chloroform-d) δ 8.18 (d, J = 7.6 Hz, 1H), 7.45-7.32
1H NMR (500 MHz, chloroform-d) δ 8.19 (d, J = 7.5 Hz, 1H), 7.39 (dd,
1H NMR (500 MHz, chloroform-d) δ 8.19 (d, J = 6.9 Hz, 1H), 7.39 (br. s.,
1H NMR (600 MHz, DMSO-d6) δ 8.74 (d, J = 5.1 Hz, 1H), 7.43 (dd,
1H NMR (500 MHz, DMSO-d6) δ 8.59 (d, J = 8.1 Hz, 1H), 7.43 (dd,
1H NMR (500 MHz, DMSO-d6) δ 8.61 (d, J = 8.1 Hz, 1H), 7.44 (dd,
1H NMR (500 MHz, methanol-d4) δ 8.90 (d, J = 7.8 Hz, 1H), 7.72 (d,
1H NMR (500 MHz, chloroform-d) δ 8.09 (d, J = 7.6 Hz, 1H), 7.26-7.20
1H NMR (500 MHz, chloroform-d) δ 8.12 (d, J = 7.3 Hz, 1H), 7.40 (dd,
1H NMR (500 MHz, chloroform-d) δ 8.17 (d, J = 7.6 Hz, 1H), 7.26-7.20
1H NMR (500 MHz, menthanol-d4) δ 8.57 (d, J = 7.8 Hz, 1H), 7.55-7.43
It will be evident to one skilled in the art that the present disclosure is not limited to the foregoing illustrative examples, and that it can be embodied in other specific forms without departing from the essential attributes thereof. It is therefore desired that the examples be considered in all respects as illustrative and not restrictive, reference being made to the appended claims, rather than to the foregoing examples, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
This application claims priority to Provisional Patent Application U.S. Ser. No. 61/879,925 filed Sep. 19, 2013, hereby incorporated by reference in its entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/US14/56273 | 9/18/2014 | WO | 00 |
| Number | Date | Country | |
|---|---|---|---|
| 61879925 | Sep 2013 | US |
