Claims
- 1. A plant cell comprising a heterologous polynucleotide encoding a gene product that is expressed in the plant cell wherein the gene product has trichothecene resistance activity.
- 2. A plant comprising a plant cell of claim 1, wherein the plant is resistant to a trichothecene.
- 3. The plant according to claim 2, wherein the plant is resistant to a trichothecene comprising a C-3 hydroxyl group.
- 4. The plant according to claim 3 wherein the gene product is a 3-O-acetyltransferase.
- 5. The plant according to claim 4, wherein the gene product is a polypeptide comprising a sequence substantially similar to SEQ ID NO:2, 6 or 8.
- 6. The plant according to claim 4, wherein the heterologous polynucleotide comprises a sequence substantially similar to SEQ ID NO:1, 5 or 7.
- 7. The plant according to claim 4, wherein the heterologous polynucleotide comprises a consecutive 18 base pair portion identical in sequence to a consecutive 18 base pair portion set forth in SEQ ID NO:1, 5 or 7.
- 8. The plant of claim 6, wherein the trichothecene is selected from the group consisting of T-2 toxin, HT-2 toxin, isotrichodermol, DAS, 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; type B: 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and DON.
- 9. The plant of claim 8 wherein the trichothecene is DAS or DON.
- 10. A seed of the plant according to claim 6.
- 11. The plant according to claim 6, wherein the plant is a wheat, maize, barley or rice plant.
- 12. The plant according to claim 6, wherein the plant is resistant to a fungus that produces a trichothecene comprising a C-3 hydroxyl group.
- 13. The plant according to claim 12, wherein the plant is resistant to Fusarium graminearum.
- 14. The plant of claim 2, wherein the heterologous polynucleotide is a microbial polynucleotide.
- 15. The plant of claim 14, wherein the microbial polynucleotide is a yeast or fungal polynucleotide.
- 16. The plant of claim 15, wherein the fungal polynucleotide is a Fusarium polynucleotide.
- 17. The plant of claim 16, wherein the Fusarium polynucleotide is a Fusarium graminearum or Fusarium sporotrichioides polynucleotide.
- 18. The plant of claim 15, wherein the plant is resistant to a fungus that produces a trichothecene.
- 19. The plant of claim 18, wherein the plant is resistant to a fungus that produces a trichothecene comprising a C-3 hydroxyl group.
- 20. The plant according to claim 18, wherein the plant is a wheat, maize or rice plant.
- 21. A seed of the plant according to claim 18.
- 22. A method for producing a trichothecene resistant plant comprising the steps of:
a) transforming a plant cell with a heterologous gene encoding a gene product, wherein the gene product increases resistance to a trichothecene; b) expressing the gene product at a biologically significant level c) regenerating the plant cell into a plant; and d) selecting a plant with increased resistance to a trichothecene.
- 23. The method of claim 22 further comprising the step of selecting a plant on which growth of fungus wherein the fungus produces a trichothecene virulence factor is reduced.
- 24. The method of claim 23, wherein the fungus is of the genera Fusarium.
- 25. A trichothecene resistant plant obtained by the method of claim 22.
- 26. A seed produced by selfing or outcrossing the plant of claim 22, wherein a plant grown from the seed has an increased resistance to a trichothecene.
- 27. A method of preventing mycotoxin crop contamination comprising growing the plant of claim 25, wherein the plant is a crop plant.
- 28. A method of preventing fungal growth on a crop, comprising growing the plant of claim 25, wherein the plant is a crop plant.
- 29. A method of selecting transformed host cells, the method comprising:
transforming a host cell with a nucleic acid construct encoding a trichothecene 3-O-acetyltransferase, growing the transformed host cell in the presence of a trichothecene.
- 30. The method of claim 29 wherein the host cell is a plant cell.
- 31. The method of claim 30, wherein the host cell is a microbial host cell.
- 32. The method of claim 31, wherein in the microbial host cell is a fungal host cell.
- 33. The method of claim 29, wherein the trichothecene is selected from the group consisting
T-2 toxin, HT-2 toxin, isotrichodermol, DAS, 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; type B: 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and DON.
- 34. The plant according to claim 6, wherein the heterologous polynucleotide comprises the nucleic acid sequence of SEQ ID NO: 1, 5 or 7.
- 35. A method for reducing the growth of a fungus on a plant that produces a trichothecene comprising a C-3 hydroxyl group comprising growing the plant of claim 12.
- 36. A method for preventing mycotoxin contamination of a plant, including of a plant's seed, comprising growing the plant of claim 12.
- 37. A method of producing a fungal resistant plant comprising selfing or outcrossing the plant of claim 12.
- 38. A method of producing seed wherein the plant grown from the seed is fungal resistant, comprising selfing or outcrossing the plant of claim 12 and collecting the seed.
- 39. The plant of claim 12, wherein the plant is resistant to Fusarium.
- 40. A method for reducing the growth of a Fusarium on a plant comprising growing the plant of claim 39.
- 41. A method for preventing mycotoxin contamination of a plant, including of the plant's seed, comprising growing the plant of claim 39.
- 42. A method of producing a Fusarium resistant plant comprising selfing or outcrossing the plant of claim 39.
- 43. A method of producing seed wherein the plant grown from the seed is resistant to Fusarium, comprising selfing or outcrossing the plant of claim 39 and collecting the seed.
- 44. A method of preventing mycotoxin crop contamination, including preventing contamination of the crop seed, comprising growing the plant of claim 39, wherein the plant is a crop plant.
- 45. Plasmid pNOV1700 (NRRL B-30117).
- 46. An isolated nucleic acid molecule having the sequence of the 4117 bp PvuII fragment in plasmid pNOV1700 (NRRL B-30117).
- 47. A plant cell comprising a nucleic acid molecule according to claim 46.
- 48. A plant comprising a plant cell according to claim 47.
- 49. A plant according to claim 48, which is wheat or corn.
Parent Case Info
[0001] This application is a continuation of U.S. application Ser. No. 09/538,414, filed Mar. 29, 2000, now U.S. Pat. No. 6,346,655, which claims the benefit of U.S. Provisional Application No. 60/304,177, filed Mar. 31, 1999, and U.S. Provisional Application No. 60/287,549, filed Feb. 11, 2000.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60304177 |
Mar 1999 |
US |
|
60287549 |
Feb 2000 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09538414 |
Mar 2000 |
US |
Child |
10074279 |
Feb 2002 |
US |