TRYPANOSOMA CRUZI GENOME PROJECT AT TIGR

Information

  • Research Project
  • 6712120
  • ApplicationId
    6712120
  • Core Project Number
    U01AI045038
  • Full Project Number
    5U01AI045038-05
  • Serial Number
    45038
  • FOA Number
  • Sub Project Id
  • Project Start Date
    3/15/2000 - 24 years ago
  • Project End Date
    8/31/2005 - 19 years ago
  • Program Officer Name
    ROGERS, MARTIN JOHN
  • Budget Start Date
    3/1/2004 - 20 years ago
  • Budget End Date
    8/31/2005 - 19 years ago
  • Fiscal Year
    2004
  • Support Year
    5
  • Suffix
  • Award Notice Date
    3/7/2004 - 20 years ago

TRYPANOSOMA CRUZI GENOME PROJECT AT TIGR

Trypanosoma cruzi is a protozoan parasite that causes a fatal illness called Chagas' disease or American typanosomiasis in humans and many nonhuman mammalian species. The World Health Organization has estimated that 18 million people are infected with T. cruzi and that 50,000 patients die each year of the disease. The basic research on T. cruzi over the past two decades has resulted in many advances in our understanding of these organisms, yet these substantial research efforts have not led to a better way to manage or control this devastating disease. Recently, progress on the human genome project, and the determination of the yeast genomic sequence, have unambiguously demonstrated the value of using genomic sequences as a foundation for designing future research efforts. Our long term goal is to sequence the genome of T. cruzi. This proposal, in conjunction with similar proposed efforts at the Seattle Biomedical Research Institute and Uppsala University, represents a large step towards that goal. We will employ a sequencing strategy consisting of two phases. The purpose of the first phase is to enhance early gene discovery and to provide markers that will be important for construction of a high-resolution sequence-ready map. In this phase we will generate approximately 10 Mb of discontinuous single-pass sequence (23 percent of the 43.5 Mb genome). This will be implemented by end-sequencing 5,000 BAC (Bacterial Artificial Chromosome) clones from an already existing library and another 5,000 BAC clones of randomly-sheared DNA (from a library to be constructed in collaboration with Dr. Pieter deJong). Telomere-proximal sequences will be identified in clones of the sheared genonmic library. During the second phase 12 Mb of contiguous T. cruzi DNA will be sequenced using a chromosome by chromosome approach and a syntenic regions conserved between T. cruzi and a related parasite (Trypanosoma brucei) will be identified and analyzed. This project will provide invaluable information and benefits at many levels, including (1) identification of genes involved in basic functions of the eukaryotic cell, (2) easy, inexpensive and fast cloning of genes encoding proteins being actively studied in laboratories around the world, (3) immediate access to genes and their products for functional/structural studies, (4) prediction of metabolic pathways on the basis of candidate genes, (4) identification of parasite-specific gene products by comparison with other genomes, and (5) a framework for future experiments aimed at comparative and integrative mapping of various trypanosomatid genomes.

IC Name
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
  • Activity
    U01
  • Administering IC
    AI
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    286220
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    856
  • Ed Inst. Type
  • Funding ICs
    NIAID:286220\
  • Funding Mechanism
  • Study Section
    GNM
  • Study Section Name
    Genome Study Section
  • Organization Name
    INSTITUTE FOR GENOMIC RESEARCH
  • Organization Department
  • Organization DUNS
  • Organization City
    ROCKVILLE
  • Organization State
    MD
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    20850
  • Organization District
    UNITED STATES