Patent Grant
10835594
A computer readable form of the Sequence Listing “3244-P47591US00_SequenceListing.txt” (20,480 bytes), submitted via EFS-WEB and created on Jul. 15, 2016, is herein incorporated by reference.
The present disclosure relates to novel methods and compositions for treating and/or preventing chlamydial infection and disease. In particular, the disclosure relates to Type III secretion injectisome proteins for administration to subjects and for use in treating and/or preventing chlamydial infection and disease.
Epidemiology of Chlamydial Infections
Chlamydia is a common sexually transmitted disease caused by the bacterium Chlamydia trachomatis (Senior 2012). According to a WHO study an estimated 92 million chlamydial infections occurred worldwide in the year 1999, affecting more women (50 million) than men (42 million). The USA Center for Disease Control and Prevention reports Chlamydia as the most frequently reported infectious disease in the USA, with an estimated 4-5 million cases reported annually. Many infected individuals remain asymptomatic in the short term but may progress to chronic infection in the medium-long term with serious consequences such as infertility and Pelvic Inflammatory Disease (PID).
Chlamydia trachomatis infection affects approximately 3% of sexually active young adults and up to 18% of high risk populations. The great majority of those infected are under 25 years of age, and if left untreated, the infection has significant consequences for female fertility.
Most Chlamydia notifications are in the 15 to 24 years age group with 73% of females and 55% of males infected in this age group. Nearly 2% of females aged 15-24 years were notified with Chlamydia in 2006. Estimates of the current infertility rate for Western countries have shown that by the end of one year of unprotected sexual intercourse, 10-15% of couples will fail to conceive with 37% and 85% of infertility in developed and developing countries respectively due to tubal factor infertility. C. trachomatis has received significant attention as a primary etiological factor, responsible for significant levels of PID and salpingitis, ectopic pregnancies, tubal infertility and epididymitis in young males.
Chlamydia are the major cause of sexually transmitted disease, and despite all current efforts, the incidence of infection continues to increase, particularly in young adults and high risk populations. The costs of Chlamydia infections are several-fold. The initial costs relate to the testing and treatment of infected individuals, to ensure that transmission of the infectious agent is minimized. The second relates to the downstream disease caused by Chlamydia infections, which includes pelvic inflammatory disease, tubal blockage and infertility, in women. The third aspect relates to the cost of implementing improved research strategies into health policies and programs. Indeed, the various Chlamydia sequale are a socio economic burden resulting in a total healthcare cost, of more than US$2 billion (1994) as estimated by the Institute of Medicine. The WHO also estimates that the direct costs of caring for those with PID could be as high as US$10 billion per annum (James et al. 2008).
Trachoma, caused by C. trachomatis serovars A, B and C, is the leading cause of infectious blindness worldwide and despite long-standing control efforts, it is estimated that more than 500 million people still are at high risk of infection, over 140 million persons are infected and about 6 million are blind in Africa, the Middle East, Central and South-East Asia, and countries in Latin America (Global WHO Alliance for the Elimination of Blinding Trachoma by 2020).
Chlamydia pneumoniae, known primarily as a respiratory pathogen, was first characterized in 1989. It is the causative agent in approximately 10% of the cases of community acquired pneumoniae in the United States and Canada. In addition to causing pharyngitis, laryngitis, and bronchitis, it has also been associated with a number of diseases such as atherosclerosis, arthritis, multiple scloresis, and Alzheimer's disease. The prevalence of infection with C. pneumoniae increases with age and it is estimated that 40-60% of adults have anti-C. pneumoniae IgG antibodies.
Biology of Chlamydiae
Within the family Chlamydiaceae there are 9 different species: Chlamydia caviae, Chlamydia abortus, Chlamydia psittaci, Chlamydia felis, Chlamydia pecorum, Chlamydia suis, Chlamydia muridarum, Chlamydia trachomatis, and Chlamydia pneumoniae.
The order Chlamydiales are Gram-negative, obligate, intracellular pathogens which display a broad host range across the animal kingdom including but not limited to amoebae, insects, fish, reptiles, birds, amphibians, koalas, domesticated animals, and humans (Polkinghorne et al. 2009). In the case of the koala, Chlamydia is the major infection and when combined with habitat loss represents a major threat to remaining populations. Multiple eukaryotic cell types may be infected by Chlamydiales including epithelial and smooth muscle cells, macrophages, dendritic cells, and various cell types of the central nervous system, but the tissue tropism of these pathogens varies based on the species. Chlamydia species also infect and causes disease in a range of animals including birds, sheep, cattle, cats and marsupials, including the koala (Polkinghorne et al. 2009).
Chlamydiae are obligate intracellular pathogens that require type III secretion (T3S) to invade cells and replicate intracellulary within a cytoplasmic vacuole called an inclusion body (Johnson et al. 2008; Fields and Hackstadt 2000; Stone et al. 2010; 2011; 2012; Toor et al. 2012). All members of the genus Chlamydia share a unique, biphasic life cycle that is initiated by attachment of the metabolically quiescent elementary body (EB) to the host cell. Once the EBs are attached to the host cell, type III secretion (T3S) is used to facilitate bacterial internalization through injection of effector proteins such as the translocated actin recruitment protein (TARP) (Clifton et al. 2004). Activation of the host MEK-ERK and PI-3 kinase pathways are also involved in Chlamydial uptake which is mediated by T3S effectors (Coombes and Mahony 2002). T3S is likely also involved in preventing phagosome endosome fusion through the secretion of unidentified effectors. The remaining intracellular portion of the life-cycle takes place within a plasma-membrane derived vacuole known as an inclusion. Once inside the inclusion, EBs transform into metabolically active reticulate bodies (RB) that becomes associated with the inclusion membrane. Interaction with the inclusion membrane allows RBs to communicate with the host cell by secretion of T3S effectors permitting Chlamydia to commandeer host cell pathways to acquire lipids, cholesterol, and other nutrients crucial for growth and replication. RB replication results in expansion of the inclusion body until some unknown stimulus signals the non-infectious RBs to transform into infectious EBs which exit the host cell either by cell lysis or a packaged released mechanism termed extrusion, leaving the host cell intact (Hybiske and Stephens 2007).
T3S is a virulence factor used by several Gram-negative bacteria including Chlamydiae, Yersinia, Salmonella, Pseudomonas and E. coli whereby effector proteins are transported from the bacterial cytosol into the host cell cytoplasm (Beeckman and Vanrampay 2009). The type III secretion system translocates effectors through the inner membrane, periplasmic space, and outer membrane in a single-step using a syringe-like apparatus known as an injectisome (Galan and Wolf-Watz 2006, Ghosh 2004). The injectisome is constructed of 20-25 proteins spanning the inner membrane, periplasm and outer membrane, extending into the extracellular milieu to allow for host cell sensing and contact. The apparatus is activated upon host cell-contact, possibly by interaction of the T3S injectisome with cholesterol and sphingolipid rich microdomains, termed lipid rafts, in the host cell membrane. At the tip of the T3S needle is the needle-tip complex that is crucial for sensing host-cell contact and initiating secretion (Markham et al. 2009; Stone et al. 2012). Upon host cell contact, the T3S apparatus injects two translocator proteins, YopB/D in Yersiniae and CopB/D in Chlamydiae, into the host cell membrane to form the translocon, a molecular pore through which secreted proteins can enter the host cell (Goure 2004; 2005). Insertion of hydrophobic translocator proteins into host cell membranes is thought to be dependent on lipid-rafts since in the absence of cholesterol, translocators do not form a pore and infection is inhibited. In Yersiniae, the needle-filament protein YscF extends from the bacterial outer-membrane and houses the needle-tip complex consisting of the sensor protein and possibly the translocators (Zauberman et al. 2008). The needle-tip protein of Yersinia, LcrV, functions in this capacity by first recognizing cell contact and also acting as an extracellular chaperone facilitating translocator insertion into the host membrane. How the needle-tip protein senses the host is unknown, however, one hypothesis is that a pre-formed tip complex consisting of LcrV (pentameric or hexameric) and the translocator protein YopB (single copy) act in concert to sense the host cell. Evidence also exists suggesting that LcrV may play an additional role inside the host cell inhibiting LPS-induced polymerization of actin and cytoskeleton rearrangement. Crystallographic analysis of LcrV revealed a dumbbell-like structure with two globular domains on either end of a “grip” formed by a conserved coiled-coil motif. In C. trachomatis the CT584 protein has been proposed as the needle tip protein based on comparisons of biophysical properties to other tip proteins, however, nothing is known about the structure or function of Chlamydial needle tip proteins (Markham et al. 2009). In Chlamydia pneumonia, Cpn0803 protein has been shown to interact with several T3S components viz. the needle filament protein, the ATPase and the multi-cargo shuttling protein CdsQ (Stone et al. 2012). X-ray crystallographic analysis of Cpn0803 has revealed a conserved N-terminal 4-helix bundle, but an overall unique fold not seen in LcrV orthologs (Stone et al. 2012). PepScan mapping and Rosetta Docking analysis predict that Cpn0803 functions on the injectisome tip as a tetramer. Collectively, these data provide strong evidence for the role of Cpn0803 as the needle-tip protein in Chlamydia pneumoniae. The needle tip proteins in chlamydial species are highly genetically conserved. The amino acid similarity between CT584 and Cpn0803 is 94% with 83% amino acid identity. For Cpn0803 and C. pecorum (G5S0239) the overall alignment score is 91% and for Cpn0803 and C. psittaci (G500989) the overall similarity is 86% and for C. psittaci and C. pecorum the alignment score is 84%. Alignment score reflects how well two sequences are aligned. Similarity between two sequences is then calculated using the optimal alignment.
Need for Vaccine
Genital tract infection with Chlamydia trachomatis is an escalating global public health concern causing considerable morbidity and socioeconomic burden worldwide. Although antibiotics are used to treat symptomatic urogenital infections, chlamydial infection remains asymptomatic in approximately 50% of infected men and 70% of infected women contributing to horizontal transmission between sexual partners. The major clinical manifestations of genital chlamydial infection in women include mucopurulent cervicitis, endometritis and pelvic inflammatory disease. Genital infection with C. trachomatis markedly enhances the risk for reproductive tract sequelae in women, including tubal factor infertility, chronic pain and ectopic pregnancy.
There is no commercially available vaccine for the prevention of Chlamydia infections in humans. Further, while vaccines have previously been developed against C. abortus in ruminants and swine, and against C. felis in cats, no vaccine currently exists for the prevention of C. pecorum infections in cattle, pigs or marsupials.
It is estimated that by age 30, half of all sexually active women may have been infected with Chlamydia. Screening for Chlamydia infections is usually recommended annually for all sexually active women under 26 years of age, pregnant women and older women with pre-disposing risk factors. Although antibiotic treatment is effective in the early stage of the disease, infections may reoccur and the availability and administration of a prophylactic vaccine will potentially reduce the socio-economic burden and more importantly the health consequences associated with Chlamydia infections.
Accordingly, a need remains for new methods to treat and/or prevent chlamydial infection and disease.
The present inventors have demonstrated that administering Cpn0803 protein alone or together with CopB and/or CopD induces an immune response that is protective against a live challenge with Chlamydia.
Accordingly, one aspect of the disclosure provides a use of an effective amount of a protein having at least 80% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof for inducing an immune response against chlamydial infection in a subject or cell in need thereof.
In one embodiment, the use is for treating or preventing chlamydial infection in a subject or cell in need thereof.
In another embodiment, the use further comprises use of (a) a protein having at least 80% sequence identity to Cpn0809 (CopB) or an immunogenic fragment or epitope thereof or (b) a protein having at least 80% sequence identity to Cpn0808 (CopD) or an immunogenic fragment or epitope thereof.
In another embodiment, the use further comprises use of (a) a protein having at least 80% sequence identity to Cpn0809 or an immunogenic fragment or epitope thereof and (b) a protein having at least 80% sequence identity to Cpn0808 or an immunogenic fragment or epitope thereof.
In one embodiment, the protein having at least 80% sequence identity to Cpn0803 is Cpn0803 or CT584.
In another embodiment, the protein having at least 80% sequence identity to Cpn0809 is Cpn0809 or CT578.
In another embodiment, the protein having at least 80% sequence identity to Cpn0808 is Cpn0808 or CT579.
In another embodiment, the use further comprises use of an adjuvant.
In one embodiment, the adjuvant is CTA-DD, Iscomatrix, interleukin-12 (IL-12), CpG oligodeoxynucleotides, alum, Montanide ISA 720 or any combination thereof.
In another embodiment, the use further comprises use of at least one additional chlamydial protein or immunogenic fragment or epitope thereof.
In one embodiment, the additional chlamydial protein is IncA, MOMP, CopB2, CopD2, CdsF, CopN or any combination thereof.
In another embodiment, the protein having at least 80% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof is for delivery by a probiotic bacteria, optionally Lactococcus lactis or Lactobacillus rhamnosus.
In yet another embodiment, the protein having at least 80% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof is for intranasal, intravaginal, ocular or systemic administration.
Another aspect of the disclosure provides a composition comprising a protein having at least 80% sequence identity to Cpn0803 or an immunogenic fragment or epitope thereof and optionally a carrier.
In one embodiment, the composition further comprises (a) a protein having at least 80% sequence identity to Cpn0809 or an immunogenic fragment or epitope thereof or (b) a protein having at least 80% sequence identity to Cpn0808 or an immunogenic fragment or epitope thereof.
In another embodiment, the composition further comprises (a) a protein having at least 80% sequence identity to Cpn0809 or an immunogenic fragment or epitope thereof and (b) a protein having at least 80% sequence identity to Cpn0808 or an immunogenic fragment or epitope thereof.
In one embodiment, the protein having at least 80% sequence identity to Cpn0803 is Cpn0803 or CT584.
In another embodiment, the protein having at least 80% sequence identity to Cpn0809 is Cpn0809 or CT578.
In another embodiment, the protein having at least 80% sequence identity to Cpn0808 is Cpn0808 or CT579.
In another embodiment, the composition comprises an adjuvant.
In one embodiment, the adjuvant is CTA-DD, Iscomatrix, interleukin-12 (IL-12), CpG oligodeoxynucleotides, alum, Montanide ISA 720 or any combination thereof.
In another embodiment, the composition further comprises at least one additional chlamydial protein or immunogenic fragment or epitope thereof.
In one embodiment, the additional chlamydial protein is IncA, MOMP, CopB2, CopD2, CdsF, CopN or any combination thereof.
In another embodiment, the composition is formulated for delivery by a probiotic bacteria, optionally Lactococcus lactis or Lactobacillus rhamnosus.
In another embodiment, the composition is formulated for intranasal, intravaginal, ocular or systemic administration.
Another aspect of the disclosure provides a use of the composition described above for treating or preventing chlamydial infection or for inducing an immune response against chlamydial infection in a subject or cell in need thereof.
Other features and advantages of the present disclosure will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples while indicating preferred embodiments of the disclosure are given by way of illustration only, since various changes and modifications within the spirit and scope of the disclosure will become apparent to those skilled in the art from this detailed description.
The term “a cell” as used herein includes a a single cell as well as a plurality of cells.
The term “adjuvant” as used herein describes a substance, which can be any substance capable of being combined with the protein, peptide, fragment, epitope or composition of this disclosure to enhance, improve or otherwise modulate an immune response in a subject without deleterious effect on the subject. An adjuvant of this disclosure can be, but is not limited to, an immunostimulatory cytokine, a SYNTEX adjuvant formulation 1 (SAF-1) composed of 5 percent (wt/vol) squalene (DASF, Parsippany, N.J.), 2.5 percent Pluronic, L121 polymer (Aldrich Chemical, Milwaukee), 0.2 percent polysorbate (Tween 80, Sigma) in phosphate-buffered saline, CTA-DD, Iscomatrix, interleukin-12 (IL-12), CpG oligodeoxynucleotides, alum, Montanide ISA 720 or any combination thereof.
Suitable adjuvants also include an aluminum salt such as aluminum hydroxide gel (alum), aluminum phosphate, or algannmulin, but may also be a salt of calcium, iron or zinc, and/or may be an insoluble suspension of acylated tyrosine, or acylated sugars, cationically or anionically derivatized polysaccharides, or polyphosphazenes.
The term “chlamydial infection” as used herein refers to an infection caused by any species belonging to the bacterial family Chlamydiaceae. Chlamydiaceae are gram negative, obligate, intracellular pathogens which display a broad host range across the animal kingdom including but not limited to amoebae, insects, fish, reptiles, birds, amphibians, koalas, domesticated animals, and humans (Polkinghorne et al. 2009). In one embodiment, “chlamydial infection” refers to infection by Chlamydia caviae, Chlamydia abortus, Chlamydia psittaci, Chlamydia felis, Chlamydia pecorum, Chlamydia suis, Chlamydia muridarum, Chlamydia trachomatis, or Chlamydia pneumoniae. In one embodiment, the infection is a Chlamydia trachomatis infection. In another embodiment, “chlamydial infection” refers to infection by Chlamydophila or Parachlamydia. Sites of chlamydial infection include, but are not limited to, the genital, ocular and respiratory tracts.
The term “Cpn0803” as used herein refers to Chlamydia pneumoniae protein Cpn0803. Cpn0803 is a Chlamydia pneumoniae type III secretion-associated protein (Protein Accession: NP_224998.1, mRNA accession: 894751). CT584 (Protein accession: AAD18941.1, mRNA accession: 4377114) is the Chlamydia trachomatis homologue to Cpn0803.
The term “Cpn0809” as used herein refers to Chlamydia pneumoniae protein Cpn0809 (Protein accession: NP_225004, mRNA accession: 894727). Cpn0809 is also referred to as “Chlamydia Outer Protein B” or “CopB”. CT578 (Protein accession: AAD18947) is the Chlamydia trachomatis homologue to Cpn0809.
The term “Cpn0808” as used herein refers to Chlamydia pneumoniae protein Cpn0808 (Protein accession: NP_225003). Cpn0808 is also referred to as “Chlamydia Outer Protein D” or “CopD”. CT579 (Protein accession: AAD18946) is the Chlamydia trachomatis homologue to Cpn0808.
As used herein, the term “effective amount” refers to an amount of a protein, immunogen or composition of this disclosure that is sufficient to produce a desired effect, which can be a therapeutic, protective and/or beneficial effect. The effective amount will vary with the age, general condition of the subject, the severity of the condition being treated, the particular agent administered, the duration of the treatment, the nature of any concurrent treatment, the pharmaceutically acceptable carrier used, and like factors within the knowledge and expertise of those skilled in the art. As appropriate, an “effective amount” in any individual case can be determined by one of ordinary skill in the art by reference to the pertinent texts and literature and/or by using routine experimentation. (See, for example, Remington, The Science And Practice of Pharmacy (20th ed. 2000)).
As used herein “effective response” or “responding effectively” means a positive or beneficial response to a particular treatment in contrast to a “lack of an effective response” which can be an ineffectual, negative or detrimental response as well as the lack of a positive or beneficial response. An effective response or lack of effective response (i.e., ineffective response) is detected by evaluation, according to known protocols, of various immune functions (e.g., cell-mediated immunity, humoral immune response, etc.) and pharmacological and biological functions as would be known in the art.
The term “epitope” as used herein refers to a molecular region on the surface of a protein or antigen capable of eliciting an immune response and of combining with the specific antibody produced by such a response.
The term “fragment” or “immunogenic fragment” as used herein refers to any portion of the proteins disclosed herein that retains immunogenic activity against Chlamydia. Whether or not the fragment retains immunogenic activity may be determined using techniques known in the art. A fragment of a polypeptide or protein of this disclosure can be produced by methods well known and routine in the art.
The term “homologue” as used herein relates to similar genes or proteins in different organisms due to an ancestral relationship and/or common ancestral gene sequence. In one embodiment, an amino acid sequence or protein is defined as a homologue of a polypeptide or fragment of the present disclosure if it shares significant homology to one of the polypeptides and/or fragments of the present disclosure. In one embodiment, significant homology means at least 75%, 80%, 85%, 90%, 95%, 98% and/or 100% sequence identity with another amino acid sequence.
The term “immunogen” as used herein refers to any substance capable of inducing an immune response. Examples of immunogens in the present application include, but are not limited to, Cpn0803, CopB and CopD and fragments and epitopes thereof. Other examples of immunogens include nucleic acids encoding the proteins, fragments and epitopes described herein.
The term “immune response” as used herein can refer to activation of either or both the adaptive and innate immune system cells such that they shift from a dormant resting state to a state in which they are able to elaborate molecules typical of an active immune response.
As used herein, the terms “elicit” or “induce” or “produce” (or grammatical variations thereof) in the context of an immune response against Chlamydia are intended to encompass the activation and/or stimulation of cells and other components of the immune system in a subject to ameliorate the effects of chlamydial infection in the subject. The immune response of this disclosure can be a protective immune response, for example, as desired in vaccination methods to treat and/or prevent infection. Protection is not required if there is some other purpose for inducing the immune response, for example, for research purposes or to produce antibody for passive immunizations or as a reagent (e.g., to detect, isolate and/or identify Chlamydia species).
The terms “immunogenic amount” or “effective immunizing dose,” as used herein, unless otherwise indicated, mean a dose of a composition of this disclosure sufficient to induce an immune response (which can be a protective response) in the treated subject that is greater than the inherent immunity of non-immunized subjects. An immunogenic amount or effective amount or effective immunizing dose in any particular context can be routinely determined using methods known in the art.
The terms “protective immunity” or “protective immune response,” as used herein, are intended to mean that the subject mounts an active immune response to the immunogenic composition and/or that the subject has been provided with passive immunity, such that upon subsequent exposure or a challenge, the animal is able to resist and/or overcome infection and/or disease. Thus, a protective immune response will decrease the incidence of morbidity and/or mortality from subsequent exposure to the chlamydial pathogens of this disclosure.
As used herein, the term “polypeptide” or “protein” is used to describe a chain of amino acids that correspond to those encoded by a nucleic acid. A polypeptide or protein of this disclosure can be a peptide, which usually describes a chain of amino acids of from two to about 30 amino acids. The term protein as used herein also describes a chain of amino acids having more than 30 amino acids and can be a fragment or domain of a protein or a full length protein. Furthermore, as used herein, the term protein can refer to a linear chain of amino acids or it can refer to a chain of amino acids that has been processed and folded into a functional protein. It is understood, however, that 30 is an arbitrary number with regard to distinguishing peptides and proteins and the terms can be used interchangeably for a chain of amino acids. The proteins of the present disclosure can be obtained by isolation and purification of the proteins from cells where they are produced naturally, by enzymatic (e.g., proteolytic) cleavage, and/or recombinantly by expression of nucleic acid encoding the proteins or fragments of this disclosure. The proteins and/or fragments of this disclosure can also be obtained by chemical synthesis or other known protocols for producing proteins and fragments.
The term “polynucleotide” and/or “nucleic acid sequence” as used herein refers to a sequence of nucleoside or nucleotide monomers consisting of naturally occurring bases, sugars and intersugar (backbone) linkages. The term also includes modified or substituted sequences comprising non-naturally occurring monomers or portions thereof. The nucleic acid sequences of the present application may be deoxyribonucleic acid sequences (DNA) or ribonucleic acid sequences (RNA) and may include naturally occurring bases including adenine, guanine, cytosine, thymidine and uracil.
The amino acid sequences of this disclosure are presented in the amino to carboxy direction, from left to right. Nucleotide sequences are presented herein, in the 5′ to 3′ direction, from left to right. The nucleic acids of this disclosure can be either single or double stranded (i.e., including the complementary nucleic acid). A nucleic acid of this disclosure can be the complement (e.g., complementary to the full length or only to a portion) of a nucleic acid described herein.
The term “sequence identity” as used herein refers to the percentage of sequence identity between two amino acid sequences or two nucleic acid sequences. To determine the percent identity of two amino acid sequences or of two nucleic acid sequences, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of a first amino acid or nucleic acid sequence for optimal alignment with a second amino acid or nucleic acid sequence). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity=number of identical overlapping positions/total number of positions·times·100%). In one embodiment, the two sequences are the same length. The determination of percent identity between two sequences can also be accomplished using a mathematical algorithm. A preferred, non-limiting example of a mathematical algorithm utilized for the comparison of two sequences is the algorithm of Karlin and Altschul, 1990, Proc. Natl. Acad. Sci. U.S.A. 87:2264-2268, modified as in Karlin and Altschul, 1993, Proc. Natl. Acad. Sci. U.S.A. 90:5873-5877. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al., 1990, J. Mol. Biol. 215:403. BLAST nucleotide searches can be performed with the NBLAST nucleotide program parameters set, e.g., for score=100, wordlength=12 to obtain nucleotide sequences homologous to a nucleic acid molecules of the present application. BLAST protein searches can be performed with the XBLAST program parameters set, e.g., to score-50, wordlength=3 to obtain amino acid sequences homologous to a protein molecule of the present disclosure. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., 1997, Nucleic Acids Res. 25:3389-3402. Alternatively, PSI-BLAST can be used to perform an iterated search which detects distant relationships between molecules (Id.). When utilizing BLAST, Gapped BLAST, and PSI-Blast programs, the default parameters of the respective programs (e.g., of XBLAST and NBLAST) can be used (see, e.g., the NCBI website). The percent identity between two sequences can be determined using techniques similar to those described above, with or without allowing gaps. In calculating percent identity, typically only exact matches are counted.
The term “subject” as used herein includes any animal susceptible to infection by a Chlamydial species. Such a subject can be a mammal (e.g., a laboratory animal such as a rat, mouse, guinea pig, rabbit, primates, etc.), a farm or commercial animal (e.g., a cow, horse, goat, donkey, sheep, etc.), a domestic animal (e.g., cat, dog, ferret, etc.), an avian species and in particular embodiments, is a human. In another embodiment, a subject is a koala.
A “subject in need thereof” is a subject known to be, or suspected of being, infected with, or at risk of being infected with, Chlamydia. A subject of this disclosure can also include a subject not previously known or suspected to be infected by Chlamydia or in need of treatment for Chlamydia infection. For example, a subject of this disclosure can be administered the proteins, immunogens, or compositions of this disclosure even if it is not known or suspected that the subject is infected with Chlamydia (e.g., prophylactically). A subject of this disclosure is also a subject known or believed to be at risk of infection by Chlamydia.
The terms “treat,” “treating” or “treatment” as used herein refer to any type of action that imparts a modulating effect, which, for example, can be a beneficial and/or therapeutic effect, to a subject afflicted with a condition, disorder, disease or illness, including, for example, improvement in the condition of the subject (e.g., in one or more symptoms), delay in the progression of the disorder, disease or illness, prevention or delay of the onset of the disease, disorder, or illness, and/or change in clinical parameters of the condition, disorder, disease or illness, etc., as would be well known in the art. The terms “treat,” “treating” or “treatment” as used herein also mean administering to a subject a therapeutically effective amount of the compositions, cells or vector constructs of the present application and may consist of a single administration, or alternatively comprise a series of applications.
As used herein, and as well understood in the art, “treatment” or “treating” is also an approach for obtaining beneficial or desired results, including clinical results. Beneficial or desired clinical results can include, but are not limited to, alleviation or amelioration of one or more symptoms or conditions, diminishment of extent of disease, stabilized (i.e. not worsening) state of disease, preventing spread of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, and remission (whether partial or total), whether detectable or undetectable. “Treatment” can also mean prolonging survival as compared to expected survival if not receiving treatment. Further any of the treatment methods or uses described herein can be formulated alone or for contemporaneous administration with other agents or therapies.
The terms “prevent,” “preventing,” and “prevention” and like terms are used herein to include imparting any level of prevention or protection which is of some benefit to a subject, such that there is a reduction in the incidence and/or the severity of the disease in a treated subject, regardless of whether the protection or reduction in incidence and/or severity is partial or complete.
The terms “reduce,” “reduced,” “reducing,” and “reduction” (and grammatical variations thereof), as used herein, describe a decrease in a chlamydial infection- or disease-related parameter or symptom that is of some therapeutic value or benefit to the subject.
The terms “vaccine,” “vaccination” and “immunization” are well-understood in the art, and are used interchangeably herein. For example, the terms vaccine, vaccination or immunization can be understood to be a process or composition that increases a subject's immune reaction to an immunogen (e.g., by providing an active immune response), and therefore its ability to resist, overcome and/or recover from infection (i.e., a protective immune response). In one embodiment, the term “vaccine” as used herein refers to a composition that prevents Chlamydial infection and/or treats Chlamydial infection.
The present inventors have demonstrated that administering Cpn0803 protein alone or together with CopB and/or CopD induces an immune response that is protective against a live challenge with Chlamydia.
Accordingly, the application discloses methods for treating or preventing chlamydial infection, comprising administering an effective amount of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof to a subject in need thereof. Also disclosed is use of an effective amount of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof for treating or preventing chlamydial infection in a subject in need thereof. Further disclosed is use of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof in the preparation of a medicament or vaccine for treating or preventing chlamydial infection in a subject in need thereof. Even further disclosed is a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof for use in treating or preventing chlamydial infection in a subject in need thereof.
The application also discloses methods of inducing an immune response against chlamydial infection, comprising administering an effective amount of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof to a subject in need thereof. Also disclosed is use of an effective amount of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof for inducing an immune response against chlamydial infection in a subject in need thereof. Further disclosed is use of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof in the preparation of a medicament or vaccine for inducing an immune response against chlamydial infection in a subject in need thereof. Even further disclosed is a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803, or an immunogenic fragment or epitope thereof for use in inducing an immune response against chlamydial infection in a subject in need thereof.
In some embodiments, the immune response includes an active (e.g., a protective) immune response. In some embodiments, the immune response includes a cellular and/or humoral immune response. In other embodiments, the immune response includes a Th1 and/or Th2 immune response to provide protection.
“Treating or preventing chlamydial infection” includes alleviation or amelioration of one or more symptoms or conditions of chlamydial infection, diminishment of the extent of chlamydial infection, stabilization (i.e. not worsening) of the state of chlamydial infection, preventing spread of chlamydial infection, delay or slowing of progression chlamydial infection, amelioration or palliation of a chlamydial infection, and remission (whether partial or total), whether detectable or undetectable. In one embodiment, “treating or preventing chlamydial infection” includes reducing and/or ameliorating at least one pathological condition associated with chlamydial infection. In another embodiment, “treating or preventing chlamydial infection” includes reducing the likelihood of female genital tract pathology, including but not limited to pelvic inflammatory disease and tubal factor infertility. In another embodiment, “treating or preventing chlamydial infection” includes reducing the likelihood of infertility due to Chlamydia infection.
In addition to Cpn0803 and fragments and epitopes thereof, also contemplated for use in the present methods are proteins having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803 and fragments and epitopes thereof. Examples of proteins having at least 80% sequence identity to Cpn0803 include homologues of Cpn0803 from other Chlamydia species.
In one embodiment, the protein having at least 80% sequence identity to Cpn0803 is a Cpn0803 homologue from Chlamydia caviae (hypothetical protein, accession #: WP_011006912), Chlamydia abortus (hypothetical protein, accession #: WP_006344529), Chlamydia psittaci (hypothetical protein, accession #: WP_014945399.1), Chlamydia felis (hypothetical protein, accession #: WP_011457611), Chlamydia pecorum (hypothetical protein, accession #: WP_013712331), Chlamydia suis (hypothetical protein, accession #: WP_035407010.1), Chlamydia muridarum (hypothetical protein, accession #: WP_010231811.1) or Chlamydia trachomatis (CT584, accession #: AAD18941.1).
In another embodiment, the protein having at least 80% sequence identity to Cpn0803 is CT584. CT584 is a Chlamydia trachomatis homologue to Cpn0803.
The present inventors have shown that administering Cpn0803 protein together with CopB and/or CopD induces an immune response that is protective against a live challenge with Chlamydia. Accordingly, in one embodiment, a protein having at least 80% sequence identity to Cpn0803 or an immunogenic fragment or epitope thereof is administered with (ii) (a) a protein having at least 80% sequence identity to Cpn0809 (CopB) or an immunogenic fragment or epitope thereof and/or (b) a protein having at least 80% sequence identity to Cpn0808 (CopD) or an immunogenic fragment or epitope thereof for treating and/or preventing chlamydial infection and/or for inducing an immune response to chlamydial infection.
Also contemplated for use in the present methods are proteins having at least 80%, 85%, 90%, 95% or 99% sequence identity to CopB or CopD. Examples of proteins having at least 80% sequence identity to CopB or CopD are CopB or CopD homologues from other Chlamydia species. In one embodiment, the protein having at least 80% sequence identity to CopB is a CopB homologue from Chlamydia caviae, Chlamydia abortus, Chlamydia psittaci, Chlamydia felis, Chlamydia pecorum, Chlamydia suis, Chlamydia muridarum or Chlamydia trachomatis. In one embodiment, the protein having at least 80% sequence identity to CopD is a CopD homologue from Chlamydia caviae, Chlamydia abortus, Chlamydia psittaci, Chlamydia felis, Chlamydia pecorum, Chlamydia suis, Chlamydia muridarum or Chlamydia trachomatis.
In one embodiment, the protein having at least 80% sequence identity to CopB is CT578. CT578 is a Chlamydia trachomatis homologue to CopB.
In another embodiment, the protein having at least 80% sequence identity to CopD is CT579. CT579 is a Chlamydia trachomatis homologue to CopD.
Cpn0803/CT584 or an immunogenic fragment or epitope thereof may be administered before, after and/or concurrent with the administration with CopB/CT578 or an immunogenic fragment or epitope thereof and/or CopD/CT579 or an immunogenic fragment or epitope thereof. In one embodiment, and as described in more detail below, Cpn0803/CT584 or an immunogenic fragment or epitope thereof is administered in a composition with CopB/CT578 or an immunogenic fragment or epitope thereof and/or CopD/CT579 or an immunogenic fragment or epitope thereof.
In one embodiment, Cpn0803/CT584 or an immunogenic fragment or epitope thereof is fused to CopB/CT578 or an immunogenic fragment or epitope thereof and/or CopD/CT579 or an immunogenic fragment or epitope thereof and the resulting fusion protein is administered to a subject in need thereof. Methods of producing fusion proteins are well known in the art. In one embodiment, a construct encoding a fusion protein is cloned into an expression vector for expression in E. coli. An example of a fusion protein useful in the methods described herein is 6×His-His-CT584-CT578(1-100)-CT579(1-100), where the N-terminal 100 amino acids of CT578 and CT579 are cloned onto the C-terminal end of CT584.
The following table sets out the amino acid sequences corresponding to the proteins described herein:
Chlamydia pneumoniae
Chlamydia pneumoniae
Chlamydia pneumoniae
Chlamydia trachomatis
Chlamydia trachomatis
Chlamydia trachomatis
Further contemplated for use in the present methods are immunogenic fragments and epitopes of the proteins described herein.
As set forth herein, the term “immunogenic fragment” means a fragment (e.g., a peptide) of a protein that can stimulate either humoral or cellular immune responses in the subject. An immunogenic fragment of this disclosure can comprise, consist essentially of and/or consist of one, two, three, four or more epitopes of a protein of this disclosure. An immunogenic fragment can be any fragment of contiguous amino acids of the described proteins (for example, Cpn0803 (or CT584), CopB (or CT578) or CopD (or CT579)) protein and can be at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500 or 550 amino acids in length. Identification of any such immunogenic fragments is routine in the art.
In further embodiments, Cpn0803 or fragments or epitopes thereof may be administered in conjunction with additional Chlamydia proteins or fragments or epitopes thereof. Other Chlamydia proteins contemplated for use in the present methods include, but are not limited to, Chlamydia trachomatis proteins. Examples of Chlamydia trachomatis proteins include major outer membrane protein (rMOMP) and inclusion membrane protein A (rincA), etc., as are known in the art. Other examples include, but are not limited to, PorB (Ifere et al., J. Microbiol. Immunol. Infect. 40:188-200 (2007))), enolase (Finco et al. Vaccine 23:1178-1188 (2005)), Cta1 (Roan et al. PNAS 103:12069-74 (2006)), CH089 (CopN), CT147 (EEA homology), CT226 (Inc), CT442 (15 kDa Crp), CT443 (60 kDa CRP, OmcB), CT529 (Inc, CapA), CT694 (HP, IB), CT795 (HP, IB), CT806CT812 (pmpD), CT813 (Inc), CT823, CT841, pCT03, CT110 (HSP60), CT806, CT823, CT841, pCTO3 and CT813. Other Chlamydia proteins contemplated for use in the present methods include homologues of CT806, CT823, CT841, pCTO3 or CT813 protein from various Chlamydia species as well as TroA, TroB, IncA, IncB and IncC (see, e.g., U.S. Pat. No. 6,746,676 to Rockey et al. and U.S. Patent Application Publication No. 2006/0034871 to Grandi et al., each of which is incorporated by reference herein), as well as any combination thereof.
The proteins, fragments and epitopes described herein may be administered as proteins, polypeptides or peptides. In another embodiment, a nucleic acid encoding the proteins, fragments and epitopes described herein can be introduced into a subject, wherein the nucleic acid is expressed and the encoded product is produced to treat or prevent Chlamydial infection and/or elicit an immune response in the subject.
In embodiments of this disclosure wherein one or more nucleic acids are administered to a subject, the nucleic acid(s) can be present as naked nucleic acid and/or in a vector or plasmid that carries the nucleic acid(s). The nucleic acid(s) and/or vectors and/or plasmids can also be in a cell (e.g., an isolated cell) that is administered to a subject.
In certain embodiments, the proteins, fragments and/or epitopes of this disclosure can be fused with a “carrier” protein or peptide to produce a fusion protein. For example, the carrier protein or peptide can be fused to a protein and/or fragment of this disclosure to increase the stability thereof (e.g., decrease the turnover rate) in the cell and/or subject. Exemplary carrier proteins include, but are not limited to, glutathione-S-transferase or maltose-binding protein or human serum albumin. The carrier protein or peptide can alternatively be a reporter protein. For example, the fusion protein can comprise a polypeptide and/or fragment of this disclosure and a reporter protein or peptide (e.g., green fluorescent protein (GFP), β-glucoronidase, β-galactosidase, luciferase, and the like) for easy detection. As a further alternative, the fusion protein attached to the polypeptides and/or fragments and a carrier protein or peptide can be targeted to a subcellular compartment of interest, i.e., to affect the co-localization of the polypeptide and/or fragment. Any suitable carrier protein as is well known in the art can be used to produce a fusion protein of this disclosure.
The present disclosure further includes isolated polypeptides, peptides, proteins and/or fragments that are substantially equivalent to those described for this disclosure. As used herein, “substantially equivalent” can refer both to nucleic acid and amino acid sequences, for example a mutant sequence, that varies from a reference sequence by one or more substitutions (e.g., substitution with conservative amino acids as are well known in the art), deletions and/or additions, the net effect of which does not result in an undesirable adverse functional dissimilarity between reference and subject sequences. In some embodiments, this disclosure can include substantially equivalent sequences that have an adverse functional dissimilarity. For purposes of the present disclosure, sequences having equivalent biological activity and equivalent expression characteristics are considered substantially equivalent.
A protein or immunogenic fragment and/or epitope thereof of this disclosure may be from Chlamydia caviae, Chlamydia abortus, Chlamydia psittaci, Chlamydia felis, Chlamydia pecorum, Chlamydia suis, Chlamydia muridarum and/or Chlamydia trachomatis in any combination. Further, they may be from any species of Chlamydia, Chlamydophila and/or Parachlamdyia.
The proteins, immunogens and/or compositions of this disclosure can be modified according to methods known in the art and/or administered with an adjuvant in order to increase antigenicity. Methods of increasing the antigenicity of a protein or peptide are well known in the art and include, but are not limited to coupling the antigen with a heterologous protein (such as globulin or β-galactosidase or human albumin) or through the inclusion of one or more adjuvants in addition to the immunogen of this disclosure. The adjuvant can be administered with the immunogen, before administration of the immunogen, after administration of the immunogen, or any combination thereof.
An adjuvant of this disclosure, such as, for example, an immunostimulatory cytokine, can be administered before, concurrent with, and/or within a few hours, several hours of an immunogenic chlamydial composition of this disclosure to a subject.
Furthermore, any combination of adjuvants, such as immunostimulatory cytokines, can be co-administered to the subject before, after and/or concurrent with the administration of the proteins, immunogens and/or compositions of this disclosure.
The proteins, immunogens and/or compositions of this disclosure may be administered in any combination and in any ratio. It is contemplated that the above-described proteins, immunogens and/or compositions can be administered to a subject or to a cell of a subject to impart a therapeutic benefit, such as eliciting an immune response. Thus, as noted above, the present disclosure provides a method of inducing, eliciting or producing an immune response in a subject, comprising administering to the subject or to a cell of the subject an effective amount of a polypeptide and/or immunogenic fragment and/or epitope of this disclosure and/or a nucleic acid comprising a nucleotide sequence encoding a polypeptide and/or immunogenic fragment and/or epitope of this disclosure, with or without an adjuvant of this disclosure. The cell of the subject can be in vivo or ex vivo and can be, but is not limited to a CD8+ T lymphocyte (e.g., a cytotoxic T lymphocyte), an MHC I-expressing antigen presenting cell, such as a dendritic cell, a macrophage and/or a monocyte. The cell can also be an antigen presenting cell or other class I MHC-expressing cell which can be contacted with the nucleic acids and/or vectors of this disclosure under conditions whereby the nucleic acid or vector is introduced into the cell by standard methods for uptake of nucleic acid and vectors. The nucleic acid encoding the polypeptide and/or fragment of this disclosure is then expressed and the polypeptide and/or fragment product is processed within the antigen presenting cell or other MHC I-expressing cell and presented on the cell surface as an MHC I/antigen complex. The antigen presenting cell or other class I MHC-expressing cell is then contacted with an immune cell of the subject which binds the class I MHC/antigen complex and elicits an immune response which treats or prevents Chlamydia infection in the subject.
The proteins, immunogens and/or compositions described herein can be administered to “prime” a subject. By “prime,” “primed” or “priming” (and grammatical variations thereof) as used herein, it is meant to initiate an active immune response that is less than protective until a second dose (booster) is given at a later time.
In another embodiment, the proteins, immunogens and/or compositions described herein can be administered as a “booster”. “Boost” or “booster” means a second immunization, after an initial (or “priming”) immunization that enhances the immune response of the subject. Therefore, in some embodiments, the disclosure provides proteins, immunogens and/or compositions that produce an anamnestic response against a Chlamydia infection, in a sensitized subject, comprising an anamnestic response-inducing amount of a Chlamydia protein immunizing component. As used herein, the term “anamnestic response” means a secondary (booster) immune response in a sensitized subject. By “sensitized subject” is meant a subject that has previously been in contact with a chlamydial antigen or antigens, either by natural exposure or by vaccination (primary immunization) with Chlamydia protein immunizing components.
In an additional embodiment, the present disclosure provides a method of providing passive immunity against chlamydial infection to a subject, comprising administering to the subject an effective amount of an antibody that specifically binds a protein, fragment or epitope described herein.
Detection of an immune response in the subject and/or in the cells of the subject can be carried out according to methods standard in the art for detecting a humoral and/or cellular immune response.
The proteins, immunogens and/or compositions described herein may be administered to, or used in, living organisms including humans, and animals. The term “subject” or “animal” as used herein refers to any member of the animal kingdom, in one embodiment a mammal such as a human being. In another embodiment, the subject is a koala.
An example of treatment of a standard patient would include an intranasal, intramuscular, intradermal, or intraperitoneal administration of the proteins, immunogens and/or compositions described herein, and optionally an adjuvant, given at various times following infection and then monitoring clinical improvement. Other methods of administration include, but are not limited to, oral, rectal, topical, inhalation (e.g., via an aerosol), buccal (e.g., sub-lingual), vaginal (e.g., vaginal ring), intraurethral, parenteral (e.g., subcutaneous, intramuscular, intradermal, intraarticular, intrapleural, intraperitoneal, intracerebral, intraarterial, or intravenous), topical (i.e., both skin and mucosal surfaces, including airway surfaces), ocular and transdermal administration. The proteins, immunogens and/or compositions can also be administered via a skin scarification method, transdermally via a patch, liquid or gel or subdermally such that the proteins, immunogens and/or compositions are released over time. In another embodiment, the proteins, immunogens and/or compositions are administered via a probiotic bacteria, optionally Lactococcus lactis or Lactobacillus rhamnosus. The most suitable route in any given case will depend, as is well known in the art, on such factors as the species, age, gender and overall condition of the subject, the nature and severity of the infection being treated or prevented and/or on the nature of the particular composition (i.e., dosage, formulation) that is being administered.
The frequency of administration of the proteins, immunogens and/or compositions of this disclosure can be as frequent as necessary to impart the desired therapeutic or protective effect. For example, the proteins, immunogens and/or compositions can be administered one, two, three, four or more times per day, one, two, three, four or more times a week, one, two, three, four or more times a month, one, two, three or four times a year or as necessary to control the condition. In some embodiments, one, two, three or four doses over the lifetime of a subject can be adequate to achieve the desired therapeutic or protective effect. In some embodiments, alternate day dosing can be employed (e.g., every other day). The amount and frequency of administration of the proteins, immunogens and/or compositions of this disclosure will vary depending on the particular condition being treated or to be prevented and the desired therapeutic or protective effect.
In some embodiments, an effective immunizing dose or immunogenic amount or effective amount can comprise one or more (e.g., two or three or four or more) doses of the proteins, immunogens and/or compositions of this disclosure at any time interval (e.g., hourly, daily, weekly, monthly, yearly, etc.) so as to achieve and/or maintain the desired level of protection and/or other therapeutic benefit.
The efficacy of treating or preventing Chlamydia infection by the methods of the present disclosure can be determined by detecting a clinical improvement as indicated by a change in the subject's symptoms and/or clinical parameters, as would be well known to one of skill in the art.
The immunogens (proteins, fragments, epitopes and nucleic acids) described herein may be formulated into pharmaceutical compositions or vaccines for administration to subjects and/or use in subjects in a form suitable for administration in vivo.
Accordingly, in one embodiment, the disclosure provides a composition comprising, consisting essentially of or consisting of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803 or an immunogenic fragment or epitope thereof. In another embodiment, the disclosure provides a composition comprising, consisting essentially of or consisting of a nucleic acid encoding a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803 or an immunogenic fragment or epitope thereof.
In another embodiment, the disclosure provides a composition comprising, consisting essentially of or consisting of a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803 or an immunogenic fragment or epitope thereof and (a) a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0809 or an immunogenic fragment or epitope thereof and/or (b) a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0808 or an immunogenic fragment or epitope thereof. In another embodiment, the disclosure provides a composition comprising, consisting essentially of or consisting of a nucleic acid encoding protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0803 or an immunogenic fragment or epitope thereof and (a) a nucleic acid encoding a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0809 or an immunogenic fragment or epitope thereof and/or (b) a nucleic acid encoding a protein having at least 80%, 85%, 90%, 95% or 99% sequence identity to Cpn0808 or an immunogenic fragment or epitope thereof.
The compositions optionally include an adjuvant, namely a substance capable of being combined with the protein, peptide, fragment, epitopes of this disclosure to enhance, improve or otherwise modulate an immune response in a subject without deleterious effect on the subject. Examples of adjuvants include, but is not limited to, an immunostimulatory cytokine, a SYNTEX adjuvant formulation 1 (SAF-1) composed of 5 percent (wt/vol) squalene (DASF, Parsippany, N.J.), 2.5 percent Pluronic, L121 polymer (Aldrich Chemical, Milwaukee), and 0.2 percent polysorbate (Tween 80, Sigma) in phosphate-buffered saline. Other possible adjuvants include CTA-DD, Iscomatrix, interleukin-12 (IL-12), CpG oligodeoxynucleotides, alum, Montanide ISA 720 or any combination thereof. Suitable adjuvants also include an aluminum salt such as aluminum hydroxide gel (alum), aluminum phosphate, or algannmulin, but may also be a salt of calcium, iron or zinc, and/or may be an insoluble suspension of acylated tyrosine, or acylated sugars, cationically or anionically derivatized polysaccharides, or polyphosphazenes.
The compositions described herein can be prepared by per se known methods for the preparation of pharmaceutically acceptable compositions that can be administered to subjects, such that an effective quantity of the immunogen is combined in a mixture with a pharmaceutically acceptable carrier. Suitable carriers are described, for example, in Remington's Pharmaceutical Sciences (Remington's Pharmaceutical Sciences, 20th ed., Mack Publishing Company, Easton, Pa., USA, 2000). On this basis, the compositions include, albeit not exclusively, solutions of the substances in association with one or more pharmaceutically acceptable carriers or diluents, and contained in buffered solutions with a suitable pH and iso-osmotic with the physiological fluids.
Suitable pharmaceutically acceptable carriers include essentially chemically inert and nontoxic compositions that do not interfere with the effectiveness of the biological activity of the pharmaceutical composition. Examples of suitable pharmaceutical carriers include, but are not limited to, water, saline solutions, glycerol solutions, ethanol, N-(1(2,3-dioleyloxy)propyl)N,N,N-trimethylammonium chloride (DOTMA), diolesylphosphotidyl-ethanolamine (DOPE), and liposomes. Such compositions should contain a therapeutically effective amount of the compound, together with a suitable amount of carrier so as to provide the form for direct administration to the patient.
Pharmaceutical compositions may also include, without limitation, lyophilized powders or aqueous or non-aqueous sterile injectable solutions or suspensions, which may further contain antioxidants, buffers, bacteriostats and solutes that render the compositions substantially compatible with the tissues or the blood of an intended recipient. Other components that may be present in such compositions include water, surfactants (such as Tween), alcohols, polyols, glycerin and vegetable oils, for example. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules, tablets, or concentrated solutions or suspensions. Proteins may be supplied, for example but not by way of limitation, as a lyophilized powder which is reconstituted with sterile water or saline prior to administration to the patient.
The compositions may be in the form of a pharmaceutically acceptable salt which includes, without limitation, those formed with free amino groups such as those derived from hydrochloric, phosphoric, acetic, oxalic, tartaric acids, etc., and those formed with free carboxyl groups such as those derived from sodium, potassium, ammonium, calcium, ferric hydroxides, isopropylamine, triethylamine, 2-ethylamino ethanol, histidine, procaine, etc.
It is further contemplated that the present disclosure provides a kit comprising the compositions of this disclosure. It would be well understood by one of ordinary skill in the art that the kit of this disclosure can comprise one or more containers and/or receptacles to hold the reagents (e.g., antibodies, antigens, nucleic acids) of the kit, along with appropriate buffers and/or diluents and/or other solutions and directions for using the kit, as would be well known in the art. Such kits can further comprise adjuvants and/or other immunostimulatory or immunomodulating agents, as are well known in the art.
The compositions and kits of the present disclosure can also include other medicinal agents, pharmaceutical agents, carriers, diluents, immunostimulatory cytokines, etc. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art.
As noted above, the compositions of this disclosure can be administered to a cell of a subject or to a subject either in vivo or ex vivo. For administration to a cell of the subject in vivo, as well as for administration to the subject, the compositions of this disclosure can be administered orally, intranasally, intravaginally, intraocularly, parenterally (e.g., intravenously), by intramuscular injection, by intraperitoneal injection, subcutaneous injection, transdermally, extracorporeally, topically or the like. Also, the compositions of this disclosure can be pulsed onto dendritic cells, which are isolated or grown from a subject's cells, according to methods well known in the art, or onto bulk peripheral blood mononuclear cells (PBMC) or various cell subfractions thereof from a subject.
In one embodiment, the compositions of this disclosure are formulated for delivery by a probiotic bacteria, optionally Lactococcus lactis or Lactobacillus rhamnosus.
The exact amount(s) of the composition(s) of this disclosure that will be required will vary from subject to subject, depending on the species, age, weight and general condition of the subject, the particular composition used, its mode of administration and the like. Thus, it is not possible to specify an exact amount for every composition of this disclosure. However, effective amount can be determined by one of ordinary skill in the art using only routine experimentation given the teachings herein and that are well known in the art.
The pharmaceutical compositions of this disclosure include those suitable for oral, intranasal, rectal, topical, inhalation (e.g., via an aerosol) buccal (e.g., sub-lingual), vaginal (e.g., vaginal ring), intraurethral, parenteral (e.g., subcutaneous, intramuscular, intradermal, intraarticular, intrapleural, intraperitoneal, intracerebral, intraarterial, or intravenous), topical (i.e., both skin and mucosal surfaces, including airway surfaces), ocular and transdermal administration. The compositions herein can also be formulated for administered via a skin scarification method or transdermally via a patch, liquid or gel. The compositions can also be formulated for delivery subdermally in the form of a biodegradable material that releases the compositions over time. The most suitable route in any given case will depend, as is well known in the art, on such factors as the species, age, gender and overall condition of the subject, the nature and severity of the infection being treated or prevented and/or on the nature of the particular composition (i.e., dosage, formulation) that is being administered.
As described above, the frequency of administration of a composition of this disclosure can be as frequent as necessary to impart the desired therapeutic or protective effect. The amount and frequency of administration of the composition of this disclosure will vary depending on the particular condition being treated or to be prevented and the desired therapeutic or protective effect.
In some embodiments, an effective immunizing dose or immunogenic amount or effective amount can comprise one or more (e.g., two or three or four or more) doses of the compositions of this disclosure at any time interval (e.g., hourly, daily, weekly, monthly, yearly, etc.) so as to achieve and/or maintain the desired level of protection and/or other therapeutic benefit.
The above disclosure generally describes the present application. A more complete understanding can be obtained by reference to the following specific examples. These examples are described solely for the purpose of illustration and are not intended to limit the scope of the application. Changes in form and substitution of equivalents are contemplated as circumstances might suggest or render expedient. Although specific terms have been employed herein, such terms are intended in a descriptive sense and not for purposes of limitation.
The following non-limiting examples are illustrative of the present application.
Serum IgA and IgG Antibody Response to rCpn0803 (
Mice were either immunized intranasally (IN) or subcutaneously (SC) with either CTA1-DD/CpG 1826 adjuvant (5 ug each) or Iscomatrix adjuvant (5 ug IN or 10 ug SC) on days 0, 7, 14 and boosted on day 28. Five Female BALB/c mice were used in each group. Blood samples were collected on day 35 and vaginal washes collected daily for 4 days from days 32-35. IgG and IgA levels were measured by ELISA and titers expressed as the reciprocal of the end point dilution.
Vaginal IgA and IgG Antibody Response to rCpn0803 (
Mice were either immunized intranasally (IN) or subcutaneously (SC) on days 0, 7, 14 and boosted on day 28. Five Female BALB/c mice were used in each group. Vaginal washes collected daily for 4 days from days 32-35. IgG and IgA levels were measured by ELISA and titers expressed as the reciprocal of the end point dilution.
Clearance of Murine C. trachomatis (Cmu) from the Vaginal Vault Following Intranasal (IN) Immunization with CTA-1DD/CpG Adjuvant (5 ug Each) and Live Challenge with Cmu (
Mice were immunized with either rMOMP or rCpn0803 as described in
Degree of Genital Tract Pathology in Mice Immunized Intransally (IN) with rMOMP or rCpn0803 (
Mice were immunized as described in
Clearance of Murine C. trachomatis (Cmu) from the Vaginal Vault Following Subcutaneous (SC) Immunization with Iscomatrix Adjuvant (10 μg) and Live Challenge with Cmu (
Mice were immunized with either rMOMP or rCpn0803 as described in
Degree of Genital Tract Pathology in Mice Immunized Subcutaneously (SC) with rMOMP or rCpn0803 and Iscomatrix Adjuvant (5 μg) (
Mice were immunized as described in
Clearance of Murine C. trachomatis (Cmu) from the Vaginal Vault Following a Combination of Intranasal (IN) Immunization with Iscomatrix Adjuvant (5 ug) and Live Challenge with Cmu (
Mice were immunized with either rMOMP or rCpn0803 as described in
Clearance of Murine C. trachomatis (Cmu) from the Vaginal Vault Following a Combination of Subcutaneous (SC) Immunization with Iscomatrix Adjuvant (10 ug) and Live Challenge with Cmu (
Mice were immunized with either rMOMP or rCpn0803 as described in
Degree of Genital Tract Pathology in Mice Immunized Subcutaneously (SC) with rMOMP or rCpn0803 and Isomatrix Adjuvant (10 μg) (
Mice were immunized as described in
Clearance of Murine C. trachomatis (Cmu) from the Vaginal Vault Following Live Infection with Cmu in the Absence of Immunization (
Following challenge with 5×104 IFU Cmu intravaginally on day 42 vaginal swabs were collected every 3 days for 21 days to assess the level of Cmu infection in McCoy cells and expressed as IFU/swab (left panel). The area under the curve (AUC) analysis (right panel) allows comparison of extent of infection by combining the intensity and duration of Cmu infection between groups.
Clearance of Murine C. trachomatis (Cmu) from the Vaginal Vault in Immunized and Un-Immunized Animals (UNIMM) (
Area Under the Curve Analysis for Various Immunization Routes and Various Adjuvants (
Data compiled from
Degree of Genital Tract Pathology in Mice Immunized Subcutaneously (SC) or Intranasally (IN) with Either rMOMP or rCpn0803 and Either CTA1-DD/CpG Adjuvant or Isomatrix Adjuvant or Unimmunized Mice (UNIMM) (
Graph represents compiled data from
Percent Inhibition of Chlamydia Infection when Pre Incubated with Antibodies to CopB and CopD (
Chlamydia pneumonia was incubated with control antibody (anti-GST), anti-CopB or anti-CopD at a 1:10 dilution prior to infection. Percent inhibition of infection is shown as compared to infection alone. Experiments were performed in triplicate; error bars represent 2 standard deviations.
C.
pneumoniae. P1, P3, P6, represent positions 1, 3, and 6,
A trivalent antigen composed of full length CT584 followed by the N-terminal 100 amino acids of CT578 and the N-terminal 100 amino acids of CT579 was constructed (
Three consecutive restriction digestions and ligations were used to insert CT584, CT578(1-100), CT579(1-100) into MCS1, which encodes an N-terminal 6×-His tag, yielding the following ˜43 kDa fusion protein: 6×His-His-CT584-CT578(1-100)-CT579(1-100).
While the present application has been described with reference to what are presently considered to be the preferred examples, it is to be understood that the application is not limited to the disclosed examples. To the contrary, the application is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims.
All publications, patents, and patent applications are herein incorporated by reference in their entirety to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety.
This application is a national phase entry of PCT/CA2015/000030 filed on Jan. 16, 2015 (which designates the U.S.) which claims the benefit of priority from U.S. Provisional Patent Application No. 61/928,117 filed on Jan. 16, 2014, the contents of which are hereby incorporated by reference in their entirety.
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CA2015/000030 | 1/16/2015 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2015/106345 | 7/23/2015 | WO | A |
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| 20060034871 | Grandi et al. | Feb 2006 | A1 |
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| 2386314 | Nov 2011 | EP |
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| Entry |
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| Number | Date | Country | |
|---|---|---|---|
| 20160346375 A1 | Dec 2016 | US |
| Number | Date | Country | |
|---|---|---|---|
| 61928117 | Jan 2014 | US |
