Claims
- 1. A process for the preparation of tyrissamycin having the following characteristics:
(a) a white amorphous powder; (b) soluble in chloroform, dimethylsulfoxide and methanol, and essentially insoluble in water: (c) exhibits an ultraviolet absorption spectrum when dissolved in methanol substantially as shown in FIG. 1; (d) exhibits an infrared absorption spectrum (KBr) substantially as shown in FIG. 2; (e) exhibits a proton magnetic resonance spectrum in CH3OD substantially as shown in FIG. 3; and (f) exhibits a 13C magnetic resonance spectrum in CH3OD substantially as shown in FIG. 4 which comprises cultivating a tyrissamycin-producing strain of Streptomyces lividans under submerged aerobic conditions in an aqueous nutrient medium containing assimilable sources of carbon and nitrogen until a substantial amount of tyrissamycin is produced by said organism in said culture medium.
- 2. The process of claim 1 which includes the step of isolating tyrissamycin from the medium in substantially purified form.
- 3. A biologically pure culture of Streptomyces lividans ATCC-202143, said culture being capable of producing the compound tyrissamycin as defined in claim 1 in a recoverable quantity upon submerged aerobic cultivation in an aqueous nutrient medium containing assimilable sources of carbon and nitrogen.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This divisional application claims the benefit U.S. Non-Provisional Application Serial No. 09/378,309 filed Aug. 20, 1999 and U.S. Provisional Application Serial No. 60/097,214 filed Aug. 20, 1998.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60097214 |
Aug 1998 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09378309 |
Aug 1999 |
US |
Child |
09968598 |
Oct 2001 |
US |