Tyrosine derivatives

Abstract

The compounds of the invention are inhibitors of alpha4 containing integrin-mediated binding to ligands such as VCAM-1 and MAdCAM.

Description

BACKGROUND OF THE INVENTION

[0002] The integrins are α/β heterodimeric cell surface receptors involved in numerous cellular processes from cell adhesion to gene regulation. Hynes, R. O., Cell, 1992, 69:11-25; Hemler, M. E., Annu. Rev. Immunol., 1990, 8:365-368. Several integrins have been implicated in disease processes and have generated widespread interest as potential targets for drug discovery. Sharar, S. R. et al., Springer Semin. Immunopathol., 1995, 16:359-378. In the immune system integrins are involved in leukocyte trafficking, adhesion and infiltration during inflammatory processes. Nakajima, H. et al., J. Exp. Med., 1994, 179:1145-1154. Differential expression of integrins regulates the adhesive properties of cells and different integrins are involved in different inflammatory responses. Butcher, E. C. et al., Science, 1996, 272:60-66. The alpha4 integrins (i.e. alpha4beta1 (α4β1) and alpha4beta7 (α4β7)) are expressed primarily on monocytes, lymphocytes, eosinophils, basophils, and macrophages but not on neutrophils. Elices, M. J. et al., Cell, 1990, 60:577-584. The primary ligands for α4 integrins are the endothelial surface proteins mucosal addressin cell adhesion molecule (MAdCAM) and vascular cell adhesion molecule (VCAM) with lower affinity. Makarem, R. et al., J. Biol. Chem., 1994, 269:4005-4011. The binding of the α4β7 or α4β1 to MAdCAM and/or VCAM expressed on high endothelial venules (HEVs) at sites of inflammation results in firm adhesion of the leukocyte to the endothelium followed by extravasation into the inflamed tissue. Chuluyan, H. E. et al., Springer Semin. Immunopathol., 1995, 16:391-404. Monoclonal antibodies directed against α4β1, α4β7, MAdCAM or VCAM have been shown to be effective modulators in animal models of chronic inflammatory diseases such as asthma (Laberge, S. et al., Am. J. Respir. Crit. Care Med., 1995, 151:822-829.), rheumatoid arthritis (RA; Barbadillo, C. et al., Springer Semin. Immunopathol., 1995, 16:375-379), colitis (Viney et al, J. Immunol., 1996, 157: 2488-2497) and inflammatory bowel diseases (IBD; Podalski, D. K., N. Eng. J. Med., 1991, 325:928-937; Powrie, F. et al., Ther. Immunol., 1995, 2:115-123).

[0003] A need exists for non-protein small molecule compounds which inhibit the interaction between the α4β7 integrin and its ligands MAdCAM and/or VCAM. These compounds are useful for treatment of chronic inflammatory diseases such as arthritis, asthma, multiple sclerosis, Crone's disease, ulcerative colitis, and Hepatitis C.

SUMMARY OF THE INVENTION

[0004] Accordingly, the present invention relates to new compounds of the formula I, II or III: 1

[0005] wherein

[0006] Z is H or lower alkyl;

[0007] A has the structure: 2

[0008] in which

[0009] B is cyanoalkyl, a carbocycle or a heterocycle optionally substituted with one or more R1 substituents;

[0010] q is 0-3;

[0011] R1, R2, R3, R4, R5 and R6 independently are hydrogen, alkyl, amino, alkylamino, dialkylamino, nitro, urea, cyano, thio, alkylthio, hydroxy, alkoxy, alkoxyalkyl, alkoxycarbonyl, alkoxycarbonylamino, aryloxycarbonylamino, alkylsulfinyl, sulfonyl, alkylsulfonyl, aralkylsulfonyl, arylsulfonyl, heteroarylsulfonyl, alkanoyl, alkanoylamino, cycloalkanoylamino, aryl, arylalkyl, halogen, or alkylphosphonyl, and R1, R2, R3, R4 and R5 are substituted with 0-3 substituents selected from the group consisting of hydroxy, carboxyl, lower alkoxycarbonyl, lower alkyl, nitro, oxo, cyano, carbocyclyl, heterocyclyl, heteroaryl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkanoylamino, lower alkylsulfinyl, lower sulfonyl, lower alkylsulfonyl, lower alkanoyl, aryl, aroyl, heterocyclylcarbonyl, halogen and lower alkylphosphonyl; or two of R1 to R5 together form a carbocycle or heterocyclic ring;

[0012] Y is H, alkoxy, alkoxyalkoxy, aryloxy, alkylaminoalkoxy, dialkylaminoalkoxy, alkylamino, arylamino, heterocyclyl or heteroarylalkyl, where each of the forgoing may be substituted or unsubstituted;

[0013] X1 is H, C(O)OR, C(O)NRaRb, C(O)R, or C(O)SR, wherein R, Ra and Rb, individually, is hydrogen or alkyl, alkoxy, aryl, heterocyclyl, heteroaryl, substituted with 0-4 substituents selected from the group consisting of halogen, hydroxy, amino, carboxyl, nitro, cyano, heterocylyl, heteroaryl, aryl, aroyl, aryloxy, aralkyl, aralkyloxy, aryloxycarbonyl, aralkyloxycarbonyl, alkylenedioxy, lower alkoxycarbonyl, lower alkyl, lower alkenyl, lower alkynyl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkylsulfinyl, lower sulfonyl, lower alkylsulfonyl, lower alkanoyl, lower alkylphosphonyl, aminosulfonyl lower alkyl, hydroxy lower alkyl, alkylsulfinyl lower alkyl, alkylsulfonyl lower alkyl, alkylthio lower alkyl, heteroarylthio lower alkyl, heteroaryloxy lower alkyl, heteroarylamino lower alkyl, halo lower alkyl, and alkoxy lower alkyl; wherein said heterocyclyl, heteroaryl, aryl, aroyl, aryloxy, aralkyl, aralkyloxy, aryloxycarbonyl and aralkyloxycarbonyl is optionally substituted with halogen, hydroxyl, amino, carboxyl, nitro, cyano, alkyl and alkoxy; and wherein Ra and Rb together with the nitrogen to which they are attached may form a heterocyclyl or heteroaryl group substituted with 0-5 substituents R or Rd; wherein Rd has the structure 3

[0014] wherein X′ is a divalent linker selected from the group consisting of C(O)NRa, C(O) or a bond;

[0015] X2 and X3 are each independently hydrogen, halogen, hydroxy, amino, carboxyl, nitro, cyano, or substituted or unsubstituted alkyl, aryl, heterocylyl, heteroaryl, aryl, aroyl, aryloxy, alkylenedioxy, lower alkyl carbonylamino, lower alkenyl carbonylamino, aryl carbonylamino, arylalkyl carbonylamino, lower alkoxy carbonylamino, lower alkylamino carbonylamino, arylamino carbonylamino, lower alkoxycarbonyl, lower alkyl, lower alkenyl, lower alkynyl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkylsulfinyl, lower sulfonyl, lower alkylsulfonyl, lower alkanoyl, lower alkylphosphonyl, aminosulfonyl lower alkyl, hydroxy lower alkyl, alkylsulfinyl lower alkyl, alkylsulfonyl lower alkyl, alkylthio lower alkyl, heteroarylthio lower alkyl, heteroaryloxy lower alkyl, heteroarylamino lower alkyl, halo lower alkyl, alkoxy lower alkyl; and wherein X1 and X2 or X3 may be bonded together to form a heterocylic or heteroaryl ring(s); or X3 and Z together form a heterobicyclic ring;

[0016] X1′, X2′, X3′ and X4′ are each independently hydrogen, halogen, hydroxy, amino, carboxyl, nitro, cyano, or substituted or unsubstituted alkyl, alkenyl, alkynyl, arylalkyl, heterocylyl, heteroaryl, aryl, aroyl, aryloxy, alkylenedioxy, lower alkyl carbonylamino, lower alkenyl carbonylamino, aryl carbonylamino, arylalkyl carbonylamino, lower alkoxy carbonylamino, lower alkylamino carbonylamino, arylamino carbonylamino, lower alkoxycarbonyl, lower alkyl, lower alkenyl, lower alkynyl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkylsulfinyl, lower sulfonyl, lower alkylsulfonyl, lower alkanoyl, lower alkylphosphonyl, aminosulfonyl lower alkyl, hydroxy lower alkyl, alkylsulfinyl lower alkyl, alkylsulfonyl lower alkyl, alkylthio lower alkyl, heteroarylthio lower alkyl, heteroaryloxy lower alkyl, heteroarylamino lower alkyl, halo lower alkyl, alkoxy lower alkyl;

[0017] or a pharmaceutically acceptable salt thereof.

[0018] These compounds inhibit the binding of α4β7 or α4β1 to MAdCAM and/or VCAM. The invention also relates to methods of making such compounds, compositions and medicaments containing the compounds and to methods of inhibiting the binding of α4β7 or α4β1 to MAdCAM and/or VCAM and to treating diseases associated with this binding.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0019] A. Definitions:

[0020] The term “alkyl”, used alone or as part of another term, for example alkylamino, alkylsulfonyl, alkylthio, etc., means a branched or unbranched, saturated or unsaturated aliphatic hydrocarbon group, having the number of carbon atoms specified, or if no number is specified, having up to and including 12 carbon atoms. “Alkyl” when used alone or as part of another term preferably means a saturated hydrocarbon chain, however also includes unsaturated hydrocarbon carbon chains such as “alkenyl” and “alkynyl”. Examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, tert-butyl, n-pentyl, 2-methylbutyl, 2,2-dimethylpropyl, n-hexyl, 2-methylpentyl, 2,2-dimethylbutyl, n-heptyl, 3-heptyl, 2-methylhexyl, and the like. The terms “lower alkyl” “C1-C6 alkyl” and “alkyl of 1 to 6 carbon atoms” are synonymous and used interchangeably. Preferred “C1-C6 alkyl” groups are methyl, ethyl, 1-propyl, isopropyl, 1-butyl or sec-butyl.

[0021] The terms “substituted alkyl” or “substituted Cn-Cm alkyl” where m and n are integers identifying the range of carbon atoms contained in the alkyl group, denotes the above alkyl groups that are substituted by one, two, three or four halogen, trifluoromethyl, hydroxy, unsubstituted and substituted C1-C7 alkoxy, protected hydroxy, amino (including alkyl and dialkyl amino), protected amino, unsubstituted and substituted C1-C7 acyloxy, unsubstituted and substituted C3-C7 heterocyclyl, unsubstituted and substituted phenoxy, nitro, carboxy, protected carboxy, unsubstituted and substituted carboalkoxy, unsubstituted and substituted acyl, carbamoyl, carbamoyloxy, cyano, methylsulfonylamino, unsubstituted and substituted benzyloxy, unsubstituted and substituted C3-C6 carbocyclyl or C1-C4 alkoxy groups. The substituted alkyl groups may be substituted once (preferably), twice or three times with the same or with different substituents.

[0022] Examples of the above substituted alkyl groups include, but are not limited to; cyanomethyl, nitromethyl, hydroxymethyl, trityloxymethyl, propionyloxymethyl, aminomethyl, carboxymethyl, carboxyethyl, carboxypropyl, alkyloxycarbonylmethyl, allyloxycarbonylaminomethyl, carbamoyloxymethyl, methoxymethyl, ethoxymethyl, t-butoxymethyl, acetoxymethyl, chloromethyl, bromomethyl, iodomethyl, trifluoromethyl, 6-hydroxyhexyl, 2,4-dichloro(n-butyl), 2-amino(iso-propyl), 2-carbamoyloxyethyl and the like. The alkyl group may also be substituted with a carbocyclyl group. Examples include cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl, and cyclohexylmethyl groups, as well as the corresponding -ethyl, -propyl, -butyl, -pentyl, -hexyl groups, etc. A preferred group of examples within the above group includes the substituted methyl group, e.g. a methyl group substituted by the same substituents as the “substituted Cn-Cm alkyl” group. Examples of the substituted methyl group include groups such as hydroxymethyl, protected hydroxymethyl (e.g. tetrahydropyranyloxymethyl), acetoxymethyl, carbamoyloxymethyl, trifluoromethyl, chloromethyl, carboxymethyl, bromomethyl and iodomethyl.

[0023] The term “alkoxy” denotes groups having the number of carbon atoms specified such as methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, t-butoxy and like groups. The term “substituted alkoxy” means these alkoxy groups substituted by the same substituents as the “substituted alkyl” group.

[0024] The term “acyloxy” denotes carboacyloxy groups having the specified number of carbon atoms such as formyloxy, acetoxy, propionyloxy, butyryloxy, pentanoyloxy, hexanoyloxy, heptanoyloxy, and the like. The term “substituted acyloxy” means these acyloxy groups substituted by the same substituents as the “substituted alkyl” group.

[0025] The term “alkylcarbonyl”, “alkanoyl” and “acyl” are used interchangeably herein encompass groups having the specified number of carbon atoms such as formyl, acetyl, propionyl, butyryl, pentanoyl, hexanoyl, heptanoyl, benzoyl and the like.

[0026] The term “alkylsulfonyl” denotes the groups —NH—SO2-alkyl, —SO2—NH-alkyl, —N—(SO2-alkyl)2 and —SO2—N(alkyl)2. Preferred alkylsulfonyl groups are —NH—SO2-Me, —NH—SO2—Et, —NH—SO2—Pr, —NH—SO2-iPr, —N—(SO2—Me)2 and —N—(SO2—Bu)2.

[0027] The term “amino” denotes primary (i.e.—NH2), secondary (i.e. —NRH) and tertiary (i.e. —NRR) amines. Preferred secondary and tertiary amines are alkylamine and dialkyl amines such as methylamine, ethylamine, propylamine, isopropylamine, dimethylamine, diethylamine, dipropylamine and disopropylamine.

[0028] The terms “carbocyclyl”, “carbocyclylic” and “carbocyclo” alone and when used as a moiety in a complex group such as a carbocycloalkyl group, refers to a mono-, bi-, or tricyclic aliphatic ring having 3 to 14 carbon atoms and preferably 3 to 7 carbon atoms. Preferred carbocyclic groups include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl groups. The terms “substituted carbocyclyl” and “carbocyclo” mean these groups substituted by the same substituents as the “substituted alkyl” group.

[0029] A “carbocycloalkyl” group is a carbocyclo group as defined above covalently bonded to an alkyl group as defined above.

[0030] The term “alkenyl” means a branched or unbranched hydrocarbon group having the number of carbon atoms designated containing one or more carbon-carbon double bonds, each double bond being independently cis, trans, or a nongeometric isomer. The term “substituted alkenyl” means these alkenyl groups substituted by the same substituents as the “substituted alkyl” group.

[0031] The term “alkynyl” means a branched or unbranched hydrocarbon group having the number of carbon atoms designated containing one or more carbon-carbon triple bonds. The term “substituted alkynyl” means these alkynyl groups substituted by the same substituents as the “substituted alkyl” group.

[0032] The terms “alkylthio” and “C1-C12 substituted alkylthio” denote C1-C12 alkyl and C1-C12 substituted alkyl groups, respectively, attached to a sulfur which is in turn the point of attachment for the alkylthio or substituted alkylthio group to the group or substituent designated.

[0033] An “alkylenedioxy” group is a —O-alkyl-O— group, where alkyl is as defined above. Preferred alkylenedioxy groups are methylenedioxy and ethylenedioxy.

[0034] The term “aryl” when used alone or as part of another term means a homocyclic aromatic group whether or not fused having the number of carbon atoms designated or if no number is designated, up to 14 carbon atoms. Preferred aryl groups include phenyl, naphthyl, biphenyl, phenanthrenyl, naphthacenyl, and the like (see e.g. Lang's Handbook of Chemistry (Dean, J. A., ed) 13th ed. Table 7-2 [1985]).

[0035] The term “aroyl” means an aryl group bonded to a carbonyl, such as benzoyl, etc.

[0036] The term “substituted phenyl” or “substituted aryl” denotes a phenyl group or aryl group substituted with one, two, three, four or five, preferably 1-2, 1-3 or 1-4 substituents chosen from halogen (F, Cl, Br, I), hydroxy, protected hydroxy, cyano, nitro, alkyl (preferably C1-C6 alkyl), alkoxy (preferably C1-C6 alkoxy), benzyloxy, carboxy, protected carboxy, carboxymethyl, protected carboxymethyl, hydroxymethyl, protected hydroxymethyl, aminomethyl, protected aminomethyl, trifluoromethyl, alkylsulfonylamino, arylsulfonylamino, heterocyclylsulfonylamino, heterocyclyl, aryl, or other groups specified. One or methyne (CH) and/or methylene (CH2) groups in these substituents may in tern be substituted with a similar group as those denoted above. Examples of the term “substituted phenyl” includes but is not limited to a mono- or di(halo)phenyl group such as 2-chlorophenyl, 2-bromophenyl, 4-chlorophenyl, 2,6-dichlorophenyl, 2,5-dichlorophenyl, 3,4-dichlorophenyl, 3-chlorophenyl, 3-bromophenyl, 4-bromophenyl, 3,4-dibromophenyl, 3-chloro-4-fluorophenyl, 2-fluorophenyl and the like; a mono- or di(hydroxy)phenyl group such as 4-hydroxyphenyl, 3-hydroxyphenyl, 2,4-dihydroxyphenyl, the protected-hydroxy derivatives thereof and the like; a nitrophenyl group such as 3- or 4-nitrophenyl; a cyanophenyl group, for example, 4-cyanophenyl; a mono- or di(lower alkyl)phenyl group such as 4-methylphenyl, 2,4-dimethylphenyl, 2-methylphenyl, 4-(isopropyl)phenyl, 4-ethylphenyl, 3-(n-propyl)phenyl and the like; a mono or di(alkoxy)phenyl group, for example, 3,4-dimethoxyphenyl, 3-methoxy-4-benzyloxyphenyl, 3-methoxy-4-(1-chloromethyl)benzyloxy-phenyl, 3-ethoxyphenyl, 4-(isopropoxy)phenyl, 4-(t-butoxy)phenyl, 3-ethoxy-4-methoxyphenyl and the like; 3- or 4-trifluoromethylphenyl; a mono- or dicarboxyphenyl or (protected carboxy)phenyl group such 4-carboxyphenyl, a mono- or di(hydroxymethyl)phenyl or (protected hydroxymethyl)phenyl such as 3-(protected hydroxymethyl)phenyl or 3,4-di(hydroxymethyl)phenyl; a mono- or di(aminomethyl)phenyl or (protected aminomethyl)phenyl such as 2-(aminomethyl)phenyl or 2,4-(protected aminomethyl)phenyl; or a mono- or di(N-(methylsulfonylamino))phenyl such as 3-(N-methylsulfonylamino))phenyl. Also, the term “substituted phenyl” represents disubstituted phenyl groups where the substituents are different, for example, 3-methyl-4-hydroxyphenyl, 3-chloro-4-hydroxyphenyl, 2-methoxy-4-bromophenyl, 4-ethyl-2-hydroxyphenyl, 3-hydroxy-4-nitrophenyl, 2-hydroxy-4-chlorophenyl, and the like, as well as trisubstituted phenyl groups where the substituents are different, for example 3-methoxy-4-benzyloxy-6-methyl sulfonylamino, 3-methoxy-4-benzyloxy-6-phenyl sulfonylamino, and tetrasubstituted phenyl groups where the substituents are different such as 3-methoxy-4-benzyloxy-5-methyl-6-phenyl sulfonylamino. Preferred substituted phenyl groups include the 2-chlorophenyl, 2-aminophenyl, 2-bromophenyl, 3-methoxyphenyl, 3-ethoxy-phenyl, 4-benzyloxyphenyl, 4-methoxyphenyl, 3-ethoxy-4-benzyloxyphenyl, 3,4-diethoxyphenyl, 3-methoxy-4-benzyloxyphenyl, 3-methoxy-4-(1-chloromethyl)benzyloxy-phenyl, 3-methoxy-4-(1-chloromethyl)benzyloxy-6-methyl sulfonyl aminophenyl groups. Also, the term “substituted phenyl” represents phenyl groups having an aryl, phenyl or heteroaryl group fused thereto. The fused ring may also be substituted with any, preferably 1, 2 or 3, of the substituents identified above for “substituted alkyl” groups.

[0037] The term “arylalkyl” means one, two, or three aryl groups having the number of carbon atoms designated, appended to an alkyl group having the number of carbon atoms designated including but not limited to; benzyl, napthylmethyl, phenethyl, benzhydryl (diphenylmethyl), trityl, and the like. A preferred arylalkyl group is the benzyl group.

[0038] The term “substituted arylalkyl” denotes an alkyl group, preferably a C1-C8alkyl group, substituted at any carbon with an aryl group, preferably a C6-C10aryl group, bonded to the alkyl group through any aryl ring position and substituted on the alkyl portion with one, two or three groups chosen from halogen (F, Cl, Br, I), hydroxy, protected hydroxy, amino, protected amino, C1-C7acyloxy, nitro, carboxy, protected carboxy, carbamoyl, carbamoyloxy, cyano, C1-C6alkylthio, N-(methylsulfonylamino) or C1-C4alkoxy. Optionally the aryl group may be substituted with one, two, three, four or five groups chosen from halogen, hydroxy, protected hydroxy, nitro, C1-C6alkyl, C1-C6alkoxy, carboxy, protected carboxy, carboxymethyl, protected carboxymethyl, hydroxymethyl, protected hydroxymethyl, aminomethyl, protected aminomethyl, or an N-(methylsulfonylamino) group. As before, when either the C1-C8 alkyl portion or the aryl portion or both are disubstituted, the substituents can be the same or different. This group may also appear as the substituted aralkyl moiety of a substituted aralkoxy group.

[0039] Examples of the term “substituted aralkyl” and this group when it occurs in a “substituted aralkoxy” group include groups such as 2-phenyl-1-chloroethyl, 1-phenyl-1-chloromethyl, 1-phenyl-1-bromomethyl, 2-(4-methoxyphenyl)ethyl, 2,6-dihydroxy-4-phenyl(n-hexyl), 5-cyano-3-methoxy-2-phenyl(n-pentyl), 3-(2,6-dimethylphenyl)n-propyl, 4-chloro-3-aminobenzyl, 6-(4-methoxyphenyl)-3-carboxy(n-hexyl), 5-(4-aminomethyl phenyl)-3-(aminomethyl)(n-pentyl), and the like.

[0040] The term “carboxy-protecting group” as used herein refers to one of the ester derivatives of the carboxylic acid group commonly employed to block or protect the carboxylic acid group while reactions are carried out on other functional groups on the compound. Examples of such carboxylic acid protecting groups include 4-nitrobenzyl, 4-methoxybenzyl, 3,4-dimethoxybenzyl, 2,4-dimethoxybenzyl, 2,4,6-trimethoxybenzyl, 2,4,6-trimethylbenzyl, pentamethylbenzyl, 3,4-methylenedioxybenzyl, benzhydryl, 4,4′-dimethoxybenzhydryl, 2,2′,4,4′-tetramethoxybenzhydryl, alkyl such as t-butyl or t-amyl, trityl, 4-methoxytrityl, 4,4′-dimethoxytrityl, 4,4′,4″-trimethoxytrityl, 2-phenylprop-2-yl, trimethylsilyl, t-butyldimethylsilyl, phenacyl, 2,2,2-trichloroethyl, beta-(trimethylsilyl)ethyl, beta-(di(n-butyl)methylsilyl)ethyl, p-toluenesulfonylethyl, 4-nitrobenzylsulfonylethyl, allyl, cinnamyl, 1-(trimethylsilylmethyl)prop-1-en-3-yl, and like moieties. The species of carboxy-protecting group employed is not critical so long as the derivatized carboxylic acid is stable to the condition of subsequent reaction(s) on other positions of the molecule and can be removed at the appropriate point without disrupting the remainder of the molecule. In particular, it is important not to subject a carboxy-protected molecule to strong nucleophilic bases or reductive conditions employing highly activated metal catalysts such as Raney nickel. (Such harsh removal conditions are also to be avoided when removing amino-protecting groups and hydroxy-protecting groups, discussed below.) Preferred carboxylic acid protecting groups are the allyl and p-nitrobenzyl groups. Similar carboxy-protecting groups used in the cephalosporin, penicillin and peptide arts can also be used to protect a carboxy group substituents. Further examples of these groups are found in T. W. Greene and P. G. M. Wuts, “Protective Groups in Organic Synthesis”, 2nd ed., John Wiley & Sons, Inc., New York, N. Y., 1991, chapter 5; E. Haslam, “Protective Groups in Organic Chemistry”, J. G. W. McOmie, Ed., Plenum Press, New York, N. Y., 1973, Chapter 5, and T. W. Greene, “Protective Groups in Organic Synthesis”, John Wiley and Sons, New York, N.Y., 1981, Chapter 5. The term “protected carboxy” refers to a carboxy group substituted with one of the above carboxy-protecting groups.

[0041] The term “hydroxy-protecting group” as used herein refers to a derivative of the hydroxy group commonly employed to block or protect the hydroxy group while reactions are carried out on other functional groups on the compound. Examples of such protecting groups include tetrahydropyranyloxy, acetoxy, carbamoyloxy, trifluoro, chloro, carboxy, bromo and iodo groups. Further examples of these groups are found in T. W. Greene and P. G. M. Wuts, “Protective Groups in Organic Synthesis”, 2nd ed., John Wiley & Sons, Inc., New York, N.Y., 1991, chapters 2-3; E. Haslam, “Protective Groups in Organic Chemistry”, J. G. W. McOmie, Ed., Plenum Press, New York, N.Y., 1973, Chapter 5, and T. W. Greene, “Protective Groups in Organic Synthesis”, John Wiley and Sons, New York, N.Y., 1981. The term “protected hydroxy” refers to a hydroxy group substituted with one of the above hydroxy-protecting groups.

[0042] The term “amino-protecting group” as used herein refers to a derivative of the groups commonly employed to block or protect an amino group while reactions are carried out on other functional groups on the compound. Examples of such protecting groups include carbamates, amides, alkyl and aryl groups, imines, as well as many N-heteroatom derivatives which can be removed to regenerate the desired amine group. Further examples of these groups are found in T. W. Greene and P. G. M. Wuts, “Protective Groups in Organic Synthesis”, 2nd ed., John Wiley & Sons, Inc., New York, N.Y., 1991, chapter 7; E. Haslam, “Protective Groups in Organic Chemistry”, J. G. W. McOmie, Ed., Plenum Press, New York, N.Y., 1973, Chapter 5, and T. W. Greene, “Protective Groups in Organic Synthesis”, John Wiley and Sons, New York, N.Y., 1981. The term “protected amino” refers to an amino group substituted with one of the above amino-protecting groups.

[0043] The term “inhibitor” means a compound which reduces or prevents the binding of an alpha4beta1 integrin to a VCAM-1 ligand or reduces or prevents the binding of an alpha4beta7 integrin to a MAdCAM-1 ligand or which reduces or prevents the initiation of a cellular response mediated by the ligand. An “effective amount” is an amount is an amount sufficient to at least partially inhibit the binding and and may be an inhibitory amount.

[0044] The terms “heterocyclic group”, “heterocyclic”, “heterocyclyl”, or “heterocyclo” alone and when used as a moiety in a complex group such as a heterocycloalkyl group, are used interchangeably and refer to any mono-, bi-, or tricyclic saturated or non-aromatically unsaturated ring having the number of atoms designated, generally from 3 to about 10 ring atoms, where the ring atoms are carbon and 1, 2, 3 or 4 nitrogen, sulfur or oxygen atoms. Typically, a 5-membered ring has 0 to 2 double bonds and 6- or 7-membered ring has 0 to 3 double bonds and the nitrogen or sulfur heteroatoms may optionally be oxidized, and any nitrogen heteroatom may optionally be quaternized. Examples include morpholinyl, pyrrolidinyl, oxiranyl, oxetanyl, tetrahydrofuranyl, 2,3-dihydrofuranyl, 2H-pyranyl, tetrahydropyranyl, thiiranyl, thietanyl, tetrahydrothietanyl, aziridinyl, azetidinyl, 1-methyl-2-pyrrolyl, piperidinyl, and 3,4,5,6-tetrahydropiperidinyl. A preferred group is the morpholinyl group.

[0045] A “heterocycloalkyl” or a “heterocycloalkenyl” group is a heterocyclo group as defined above covalently bonded to an alkyl or alkenyl group as defined above.

[0046] Unless otherwise specified, “heteroaryl” alone and when used as a moiety in a complex group such as a heteroaralkyl group, refers to any mono-, bi-, or tricyclic aromatic ring system having the number of atoms designated where at least one ring is a 5-, 6- or 7-membered ring containing from one to four heteroatoms selected from the group nitrogen, oxygen, and sulfur, and preferably at least one heteroatom is nitrogen (Lang's Handbook of Chemistry, supra). Included in the definition are any bicyclic groups where any of the above heteroaryl rings are fused to a benzene ring. Heteroaryls in which nitrogen or oxygen is the heteroatom are preferred.

[0047] The following ring systems are examples of the heteroaryl (whether substituted or unsubstituted) groups denoted by the term “heteroaryl”: thienyl, furyl, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, triazolyl, thiadiazolyl, oxadiazolyl, tetrazolyl, thiatriazolyl, oxatriazolyl, pyridyl, pyrimidyl, pyrazinyl, pyridazinyl, thiazinyl, oxazinyl, triazinyl, thiadiazinyl, oxadiazinyl, dithiazinyl, dioxazinyl, oxathiazinyl, tetrazinyl, thiatriazinyl, oxatriazinyl, dithiadiazinyl, imidazolinyl, dihydropyrimidyl, tetrahydropyrimidyl, tetrazolo[1,5-b]pyridazinyl and purinyl, as well as benzo-fused derivatives, for example benzoxazolyl, benzofuryl, benzothiazolyl, benzothiadiazolyl, benzotriazolyl, benzoimidazolyl and indolyl.

[0048] Heterocyclic 5-membered ring systems containing a sulfur or oxygen atom and one to three nitrogen atoms are also suitable for use in the instant invention. Examples of such preferred groups include thiazolyl, in particular thiazol-2-yl and thiazol-2-yl N-oxide, thiadiazolyl, in particular 1,3,4-thiadiazol-5-yl and 1,2,4-thiadiazol-5-yl, oxazolyl, preferably oxazol-2-yl, and oxadiazolyl, such as 1,3,4-oxadiazol-5-yl, and 1,2,4-oxadiazol-5-yl. A group of further preferred examples of 5-membered ring systems with 2 to 4 nitrogen atoms include imidazolyl, preferably imidazol-2-yl; triazolyl, preferably 1,3,4-triazol-5-yl; 1,2,3-triazol-5-yl, 1,2,4-triazol-5-yl, and tetrazolyl, preferably 1H-tetrazol-5-yl. A preferred group of examples of benzo-fused derivatives are benzoxazol-2-yl, benzthiazol-2-yl and benzimidazol-2-yl.

[0049] Further suitable specific examples of the above heterocylic ring systems are 6-membered ring systems containing one to three nitrogen atoms and optionally a sulfur or oxygen atom. Such examples include pyridyl, such as pyrid-2-yl, pyrid-3-yl, and pyrid-4-yl; pyrimidyl, preferably pyrimid-2-yl and pyrimid-4-yl; triazinyl, preferably 1,3,4-triazin-2-yl and 1,3,5-triazin-4-yl; pyridazinyl, in particular pyridazin-3-yl, and pyrazinyl. The pyridine N-oxides and pyridazine N-oxides and the pyridyl, pyrimid-2-yl, pyrimid-4-yl, pyridazinyl and the 1,3,4-triazin-2-yl groups, are a preferred group. The substituents for the optionally substituted heterocyclic ring systems, and further examples of the 5- and 6-membered ring systems discussed above can be found in W. Druckheimer et al., U.S. Pat. No. 4,278,793.

[0050] A particularly preferred group of “heteroaryl” include; 1,3-thiazol-2-yl, 4-(carboxymethyl)-5-methyl-1,3-thiazol-2-yl, 4-(carboxymethyl)-5-methyl-1,3-thiazol-2-yl sodium salt, 1,2,4-thiadiazol-5-yl, 3-methyl-1,2,4-thiadiazol-5-yl, 1,3,4-triazol-5-yl, 2-methyl-1,3,4-triazol-5-yl, 2-hydroxy-1,3,4-triazol-5-yl, 2-carboxy-4-methyl-1,3,4-triazol-5-yl sodium salt, 2-carboxy-4-methyl-1,3,4-triazol-5-yl, 1,3-oxazol-2-yl, 1,3,4-oxadiazol-5-yl, 2-methyl-1,3,4-oxadiazol-5-yl, 2-(hydroxymethyl)-1,3,4-oxadiazol-5-yl, 1,2,4-oxadiazol-5-yl, 1,3,4-thiadiazol-5-yl, 2-thiol-1,3,4-thiadiazol-5-yl, 2-(methylthio)-1,3,4-thiadiazol-5-yl, 2-amino-1,3,4-thiadiazol-5-yl, 1H-tetrazol-5-yl, 1-methyl-1H-tetrazol-5-yl, 1-(1-(dimethylamino)eth-2-yl)-1H-tetrazol-5-yl, 1-(carboxymethyl)-1H-tetrazol-5-yl, 1-(carboxymethyl)-1H-tetrazol-5-yl sodium salt, 1-(methylsulfonic acid)-1H-tetrazol-5-yl, 1-(methylsulfonic acid)-1H-tetrazol-5-yl sodium salt, 2-methyl-1H-tetrazol-5-yl, 1,2,3-triazol-5-yl, 1-methyl-1,2,3-triazol-5-yl, 2-methyl-1,2,3-triazol-5-yl, 4-methyl-1,2,3-triazol-5-yl, pyrid-2-yl N-oxide, 6-methoxy-2-(n-oxide)-pyridaz-3-yl, 6-hydroxypyridaz-3-yl, 1-methylpyrid-2-yl, 1-methylpyrid-4-yl, 2-hydroxypyrimid-4-yl, 1,4,5,6-tetrahydro-5,6-dioxo-4-methyl-as-triazin-3-yl, 1,4,5,6-tetrahydro-4-(formylmethyl)-5,6-dioxo-astriazin-3-yl, 2,5-dihydro-5-oxo-6-hydroxy-astriazin-3-yl, 2,5-dihydro-5-oxo-6-hydroxy-as-triazin-3-yl sodium salt, 2,5-dihydro-5-oxo-6-hydroxy-2-methyl-astriazin-3-yl sodium salt, 2,5-dihydro-5-oxo-6-hydroxy-2-methyl-as-triazin-3-yl, 2,5-dihydro-5-oxo-6-methoxy-2-methyl-as-triazin-3-yl, 2,5-dihydro-5-oxo-as-triazin-3-yl, 2,5-dihydro-5-oxo-2-methyl-as-triazin-3-yl, 2,5-dihydro-5-oxo-2,6-dimethyl-as-triazin-3-yl, tetrazolo[1,5-b]pyridazin-6-yl and 8-aminotetrazolo[1,5-b]-pyridazin-6-yl.

[0051] An alternative group of “heteroaryl” includes; 4-(carboxymethyl)-5-methyl-1,3-thiazol-2-yl, 4-(carboxymethyl)-5-methyl-1,3-thiazol-2-yl sodium salt, 1,3,4-triazol-5-yl, 2-methyl-1,3,4-triazol-5-yl, 1H-tetrazol-5-yl, 1-methyl-1H-tetrazol-5-yl, 1-(1-(dimethylamino)eth-2-yl)-1H-tetrazol-5-yl, 1-(carboxymethyl)-1H-tetrazol-5-yl, 1-(carboxymethyl)-1H-tetrazol-5-yl sodium salt, 1-(methylsulfonic acid)-1H-tetrazol-5-yl, 1-(methylsulfonic acid)-1H-tetrazol-5-yl sodium salt, 1,2,3-triazol-5-yl, 1,4,5,6-tetrahydro-5,6-dioxo-4-methyl-as-triazin-3-yl, 1,4,5,6-tetrahydro-4-(2-formylmethyl)-5,6-dioxo-as-triazin-3-yl, 2,5-dihydro-5-oxo-6-hydroxy-2-methyl-as-triazin-3-yl sodium salt, 2,5-dihydro-5-oxo-6-hydroxy-2-methyl-as-triazin-3-yl, tetrazolo[1,5-b]pyridazin-6-yl, and 8-aminotetrazolo[1,5-b]pyridazin-6-yl.

[0052] The term “lower” when used with a term such as alkyl to form “lower alkyl”, for example, means containing from 1 to 6 carbon atoms.

[0053] “Pharmaceutically acceptable salts” include both acid and base addition salts. “Pharmaceutically acceptable acid addition salt” refers to those salts which retain the biological effectiveness and properties of the free bases and which are not biologically or otherwise undesirable, formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, carbonic acid, phosphoric acid and the like, and organic acids may be selected from aliphatic, cycloaliphatic, aromatic, araliphatic, heterocyclic, carboxylic, and sulfonic classes of organic acids such as formic acid, acetic acid, propionic acid, glycolic acid, gluconic acid, lactic acid, pyruvic acid, oxalic acid, malic acid, maleic acid, maloneic acid, succinic acid, fumaric acid, tartaric acid, citric acid, aspartic acid, ascorbic acid, glutamic acid, anthranilic acid, benzoic acid, cinnamic acid, mandelic acid, embonic acid, phenylacetic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicyclic acid and the like.

[0054] “Pharmaceutically acceptable base addition salts” include those derived from inorganic bases such as sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, aluminum salts and the like. Particularly preferred are the ammonium, potassium, sodium, calcium and magnesium salts. Salts derived from pharmaceutically acceptable organic nontoxic bases includes salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, 2-diethylaminoethanol, trimethamine, dicyclohexylamine, lysine, arginine, histidine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperizine, piperidine, N-ethylpiperidine, polyamine resins and the like. Particularly preferred organic non-toxic bases are isopropylamine, diethylamine, ethanolamine, trimethamine, dicyclohexylamine, choline, and caffeine.

[0055] The term “prodrug” as used herein means a derivative of a parent drug molecule that enhances pharmaceutically desirable characteristics or properties (e.g. transport, bioavailablity, pharmacodynamics, etc.) and that requires biotransformation, either spontaneous or enzymatic, within the organism to release the active parent drug.

[0056] The following definitions are used herein:

[0057] DIPC: diisopropylcarbodiimide

[0058] DMAP: dimethylaminopyridine

[0059] FMOC: fluorenylmethoxycarbonyl

[0060] DMA: dimethylacetamide

[0061] HBTU: 2-(H-benzotriazole)-1-yl-1,1,3,3-tetramethyluronium hexafluorophosphate

[0062] HOBT: N-hydroxy benzotriazole

[0063] TFA: trifluoracetic acid

[0064] HPLC: high pressure liquid chromatography

[0065] NMM: N-methylmorpholine

[0066] DIPEA: diisopropyethylamine

[0067] DCM: dichloromethane

[0068] THF: tetrahydrofuran

[0069] NMP: N-methylpyrolidone

[0070] CDI: carbonyldiimidazole

[0071] B. Preferred Embodiments

[0072] The compounds of the invention have the general structures I, II and III shown below. 4

[0073] where A, Z, Y, X1, X2, X3 and X4 are as defined above, both generally and preferably.

[0074] The compounds of the invention contain one or more asymmetric carbon atoms. Accordingly, the compounds may exist as diasteriomers, enantiomers or mixtures thereof. The syntheses described above may employ racemates, diasteriomers or enantiomers as starting materials or as intermediates. Diasteriomeric compounds may be separated by chromatographic or crystallization methods. Similarly, enatiomeric mixtures may be separated using the same techniques or others known in the art. Each of the asymmetric carbon atoms may be in the R or S configuration and both of these configurations are within the scope of the invention. Compounds having the S configuration are preferred.

[0075] In one preferred embodiment, X1 in structure I is C(O)OR, C(O)R, or C(O)SR, more preferably C(O)NRaRb, with the remaining variables A, Z, Y, X2, X3 and X4 having any of the definitions given above. The X1 group is preferably in the para position relative to the point of ring attachment, but may also be preferably in the meta position. Ra and Rb together with the nitrogen to which they are attached may preferably form a 5-membered or 6-membered heterocyclyl or heteroaryl group substituted with 0-5 substituents R. The heterocyclyl or heteroaryl ring system will preferably contain one nitrogen atom, but may also preferably contain another nitrogen or an oxygen atom in the ring system. The hetero ring systems may contain fused heterocyclyl or heteroaryl rings or a combination of both and the rings may be substituted or unsubstitued. Representative examples of suitable specific heterocyclyl and heteroaryl groups are: 5

[0076] In a particular embodiment, X1 is any one of the groups shown in table 2 below which is designated as substituent R when combined with the carbonyl from which it depends.

[0077] R, Ra and Rb may also be non-cyclic, for example an hydrogen or alkyl, aryl, heterocyclyl, heteroaryl, substituted with 0-4 substituents selected from the group consisting of halogen, hydroxy, amino, carboxyl, nitro, cyano, heterocylyl, heteroaryl, aryl, aroyl, aryloxy, alkylenedioxy, lower alkoxycarbonyl, lower alkyl, lower alkenyl, lower alkynyl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkylsulfinyl, lower sulfonyl, lower alkylsulfonyl, lower alkanoyl, lower alkylphosphonyl, aminosulfonyl lower alkyl, hydroxy lower alkyl, alkylsulfinyl lower alkyl, alkylsulfonyl lower alkyl, alkylthio lower alkyl, heteroarylthio lower alkyl, heteroaryloxy lower alkyl, heteroarylamino lower alkyl, halo lower alkyl, alkoxy lower alkyl; optionally substituted as described above. Preferred groups are substituted and unsubstituted lower alkyl, lower alkenyl, aryl, and aryl lower alkyl. Some representative examples of such R, Ra and Rb groups are shown below: 67

[0078] In a particular embodiment, A has the structure: 8

[0079] in which

[0080] B is cyanoalkyl, a carbocycle or a heterocycle optionally substituted with one or more R1 substituents;

[0081] q is 0-3;

[0082] R1, R2, R3, R4, R5 and R6 independently are hydrogen, alkyl, amino, alkylamino, dialkylamino, nitro, urea, cyano, thio, alkylthio, hydroxy, alkoxy, alkoxyalkyl, alkoxycarbonyl, alkoxycarbonylamino, aryloxycarbonylamino, alkylsulfinyl, sulfonyl, alkylsulfonyl, aralkylsulfonyl, arylsulfonyl, heteroarylsulfonyl, alkanoyl, alkanoylamino, cycloalkanoylamino, aryl, arylalkyl, halogen, or alkylphosphonyl, and R1, R2, R3, R4 and R5 are substituted with 0-3 substituents selected from the group consisting of hydroxy, carboxyl, lower alkoxycarbonyl, lower alkyl, nitro, oxo, cyano, carbocyclyl, heterocyclyl, heteroaryl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkanoylamino, lower alkylsulfinyl, lower sulfonyl, lower alkylsulfonyl, lower alkanoyl, aryl, aroyl, heterocyclylcarbonyl, halogen and lower alkylphosphonyl; or two of R1 to R5 together form a carbocycle or heterocyclic ring. In a preferred embodiment, A is the group 9

[0083] where preferably R1, R5 or both R1 and R5 are not hydrogen. That is, preferred A groups are ortho-substituted benzoyl groups. Particularly preferred ortho substituents are chloro, bromo, amino and hydroxy. In addition to R1 and/or R5, the phenyl ring of the benzoyl may preferably have one or two additional substituents at R2, R3 or R4. Preferred R1, R2, R3 R4, and R5 include nitro, halogen (Cl, Br, F, I), amino, aryl, lower alkyl, lower alkylthio, lower alkoxy, lower alkylamino, lower alkyl sulfinyl, lower alkylsulfonyl, lower alkanoyl, and lower alkylphosphonyl, which may each be substituted or unsubstituted. Some representative examples include: 10

[0084] In a particular embodiment, A is any one of the groups shown in table 2 which is designated as substituent R′.

[0085] Y is preferably OH or an ester or pharmaceutically acceptable carboxylic acid salt thereof. Preferred esters are substituted or unsubstituted alkyl, alkenyl, aryl, and aryl alkyl esters.

[0086] Z is preferably hydrogen.

[0087] Preferred X2, X3 and X4 include halogen, alkyl, amino, alkylamino, and alkyl carbonylamino, the alkyl group of which may be substituted or unsubstituted. For compounds having structure I, X2 and X3 are more preferably hydrogen. For compounds having structure II, X2, X3 and X4 are more preferably hydrogen.

[0088] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, and Y is OH or a salt or prodrug thereof.

[0089] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, Y is OH, and R3, R5, X2, and X3 are all hydrogen or a salt or prodrug thereof.

[0090] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, Y is OH, Ra and Rb together with the nitrogen atom to which they are attached form a substituted or unsubstituted 5-membered or 6-membered heterocyclic or heteroaromatic ring; R3, R5, X2, and X3 are all hydrogen, or a salt or prodrug thereof.

[0091] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, Y is OH, Ra and Rb together with the nitrogen atom to which they are attached form a substituted or unsubstituted 5-membered or 6-membered heterocyclic ring containing up to 2 additional nitrogen atoms, oxygen atoms or a combination thereof; R2, R3 R4, R5, X2, and X3 are all hydrogen, or a salt or prodrug thereof.

[0092] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, Y is OH, Ra and Rb together with the nitrogen atom to which they are attached form an unsubstituted 5-membered or 6-membered heterocyclic ring or such a ring substituted with 1-3 lower alkoxy, lower alkylamino, lower alkyl, lower alkoxycarbonyl, lower alkylenedioxy, lower alkylthio, lower alkenyl, lower cyanoalkyl, phenyl, phenoxy or halo groups; R2, R3 R4, R5, X2, and X3 are all hydrogen, or a salt or prodrug thereof.

[0093] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, Y is OH, Ra and Rb, are independently, substituted or unsubstituted alkyl, aryl, arylalkyl, heterocylyl, heteroaryl, heterocyclylalkyl, heteroarylalkyl or cycloalkylalkyl; R3, R5, X2, and X3 are all hydrogen, or a salt or prodrug thereof.

[0094] In another embodiment, preferred compounds have structure I, the S configuration, the OX1 group is in the 4-position on the phenyl ring, Z is hydrogen, X1 is C(O)NRaRb, Y is OH, Ra and Rb, are independently, substituted or unsubstituted alkyl, aryl, arylalkyl, heterocylyl, heteroaryl, heterocyclylalkyl, heteroarylalkyl or cycloalkylalkyl; R2, R3 R4, R5, X2, and X3 are all hydrogen, or a salt or prodrug thereof.

[0095] C. Uses

[0096] The compounds of the invention inhibit the binding of alpha4beta1 and alpha4beta7 on lymphocytes, eosinophiles, basophiles and monocytes to a cell expressing VCAM-1 and/or MAdCAM on the cell surface. The inhibitory compounds of the invention are useful to prevent the interaction of an epithelial cell bearing VCAM-1 and/or MAdCAM on the cell surface with a leukocyte cell bearing alpha4beta1 and/or alpha4beta7 on the surface by contacting the epithelial cell or the leukocyte with an inhibitory amount of the compound of the invention. The compounds are useful in assays to determine the inhibitory effect of a compound which antagonizes the binding of alpha4beta1 and/or alpha4beta7 integrin to VCAM-1 ligand and/or MAdCAM ligand. The inhibitory compound may be a small molecule, a protein or peptide or an antibody. In an in vitro assay, the ligand or the integrin may be directly or indirectly bound to a surface, such as microtiter plate, using known methods described for example in WO 9820110, WO 9413312, WO 9624673, WO 9806248, WO 9936393, and WO 9910312. The other member of the binding pair, e.g. the integrin or the ligand, respectively, (or a cell expressing the same on its surface) is then added to the surface bound member and the inhibitory effect of a test molecule is determined. The inhibitory activity of the compounds of the invention can also be determined with this type of assay.

[0097] The binding of the integrins to their respective ligands is known to be involved in inflammatory conditions associated with leukocyte infiltration of tissues lined with epithelial cells expressing VCAM-1 or MAdCAM. Such tissues include the gastrointestinal tract, skin, urinary tract, respiratory airways and joint synovial tissues. The compounds of the invention are useful in treating diseases in which such binding is implicated as a cause of the disease or symptoms of the disease. Undesired disease symptoms may arise from cell adhesin and/or cell activation which releases proinflammatory mediators, typically when there is an increase or upregulation in the expression of VCAM-1 and/or MAdCAM on the surface of endothelial cells. Various disease states which can be treated and for which the inflammatory symptoms can be reduced upon administration of the compounds of the invention include rheumatoid arthritis, asthma, psoriasis, multiple sclerosis, inflammatory bowel disease including ulcerative colitis, pouchitis and Crohn's disease, Celiac disease, nontropical Sprue, graft-versus-host disease, pancreatitis, insulin-dependent diabetes mellitus, mastitis, cholecystitis, pericholangitis, chronic sinusitis, chronic bronchitis, pneumonitis, collagen disease, eczema, and systemic lupus erythematosis. The compounds of the invention are useful in treating these diseases and conditions by inhibiting the integrin/ligand binding.

[0098] The compounds of the invention can be assayed for ability to block the alpha4beta7/MAdCAM-1 or alpha4beta1/VCAM-1 binding interaction by addition of serial dilutions of the samples to plates with the receptors as follows. 96-well plates are coated with mouse anti-human alpha4 (31470D, PharMingen, San Diego, Calif.). The plates are decanted and blocked with 0.5% BSA. After washing alpha4beta7 or alpha4beta1 is added, followed by incubation for 2 h at room temperature. The plates are washed and samples of the small molecule antagonists are added to the plates with MAdCAM-1-Ig-HRP or VCAM-1-Ig-HRP for 2 h at room temperature. After an additional wash, the bound MAdCAM-1-Ig-HRP or VCAM-1-Ig-HRP is detected by addition of tetramethylbenzidine (TMB, Kirkegaard & Perry, Gaithersberg, Md.), followed by detection of the absorbance of the product.

[0099] Alternatively, the compounds can be assayed using any known protein-protein or cell-based assay method, such as those described, for example, in WO 99/10312 (examples 179-180) and WO 99/36393 (RPMI-CS-1 cell adhesion assay); Cardarelli et al., 1994, J. Biol. Chem., 269:18668-18673; and Viney et al, J. Immunol., 1996, 157: 2488-2497 (cell adhesion assay).

[0100] For example, 96-well ELISA plates are coated overnight at 4° C. with 2 μg/ml with anti-human CD49d, (31470D, PharMingen, San Diego, Calif.) in phosphate buffered saline. The plates are decanted and blocked with assay buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM MnCl2, 0.05% Tween-20 and 0.5% BSA) at room temperature for one hour, with gentle shaking. The plates are washed three times (in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM MnCl2, 0.05% Tween-20) and 2 μg/ml of the desired integrin (Genentech, Inc.) in assay buffer is added, followed by incubation at room temperature for two hours, with gentle shaking. After washing three times, 50 μl of samples of the small molecule antagonists (serial dilutions from 10 mM stocks in 100% DMSO) are added to the plates with 50 μl of 1 μg/ml MAdCAM-1-Ig-HRP or VCAM-1-Ig-HRP (Genentech, Inc) in assay buffer. The plates are incubated two hours at room temperature, with gentle shaking, followed by washing six times. The bound MAdCAM-1-Ig-HRP or VCAM-1-Ig-HRP is detected by addition of the peroxidase substrate, 3, 3′, 5, 5′, tetramethylbenzidine (TMB, Kirkegaard & Perry, Gaithersberg, Md.), for 10 minutes, followed by addition of 1M phosphoric acid to stop the reaction. The absorbance of the solutions are read at 450 nm on a plate reader.

[0101] Suitable animal models exist for many diseases and conditions which can be treated with the compounds of the invention. Additional confirmation of the efficacy of these compounds in specific diseases and at desired doses can be assayed using these established models. For example, animal models of chronic inflammatory diseases such as asthma (Laberge, S. et al., Am. J. Respir. Crit. Care Med., 1995, 151:822-829.), rheumatoid arthritis (RA; Barbadillo, C. et al., Springer Semin. Immunopathol., 1995, 16:375-379), colitis (Viney et al, J. Immunol., 1996, 157: 2488-2497) and inflammatory bowel diseases (IBD; Podalski, D. K., N. Eng. J. Med., 1991, 325:928-937; Powrie, F. et al., Ther. Immunol., 1995, 2:115-123) may be used to demonstrate the activity of the compounds of the invention and to conduct dose and efficacy studies.

[0102] The invention also includes pharmaceutical compositions or medicaments containing the compounds of the invention and a therapeutically inert carrier or excipient, as well as methods of using the compounds of the invention to prepare such compositions and medicaments. Typically, the inhibitors used in the method of this invention are formulated by mixing at ambient temperature at the appropriate pH, and at the desired degree of purity, with physiologically acceptable carriers, i.e., carriers that are non-toxic to recipients at the dosages and concentrations employed into a galenical administration form. The pH of the formulation depends mainly on the particular use and the concentration of compound, but preferably ranges anywhere from about 3 to about 8. Formulation in an acetate buffer at pH 5 is a suitable embodiment.

[0103] The inhibitory compound for use herein is preferably sterile. The compound ordinarily will be stored as a solid composition, although lyophilized formulations or aqueous solutions are acceptable.

[0104] The composition of the invention will be formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. The “effective amount” of the compound to be administered will be governed by such considerations, and is the minimum amount necessary to prevent, ameliorate, or treat the alpha4 mediated disorder. Such amount is preferably below the amount that is toxic to the host or renders the host significantly more susceptible to severe infection.

[0105] As a general proposition, the initial pharmaceutically effective amount of the inhibitor administered parenterally per dose will be in the range of about 0.01-100 mg/kg, preferably about 0.1 to 20 mg/kg of patient body weight per day, with the typical initial range of compound used being 0.3 to 15 mg/kg/day. Oral unit dosage forms, such as tablets and capsules, preferably contain from about 25 to about 1000 mg of the compound of the invention.

[0106] The compound of the invention may be administered by any suitable means, including oral, topical, transdermal, parenteral, subcutaneous, intraperitoneal, intrapulmonary, and intranasal, and, if desired for local immunosuppressive treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration.

[0107] An example of a suitable oral dosage form is a tablet containing 25 mg, 50 mg, 100 mg, 250 mg, or 500 mg of the compound of the invention compounded with about 90-30 mg anhydrous lactose, about 540 mg sodium croscarmellose, about 5-30 mg polyvinylpyrrolidone (PVP) K30, and about 1-10 mg magnesium stearate. The powdered ingredients are first mixed together and then mixed with a solution of the PVP. The resulting composition can be dried, granulated, mixed with the magnesium stearate and compressed to tablet form using conventional equipment. An aerosol formulation can be prepared by dissolving the compound, for example 5-400 mg, of the invention in a suitable buffer solution, e.g. a phosphate buffer, adding a tonicifier, e.g. a salt such sodium chloride, if desired. The solution is typically filtered, e.g. using a 0.2 micron filter, to remove impurities and contaminants.

[0108] D. Methods of Making

[0109] Synthesis of Acyltyrosine Compounds (I)

[0110] I. Solid Phase Synthesis:

[0111] The compounds of invention are prepared from tyrosine and tyrosine derivatives using known chemical reactions and according to the method shown below. 11

[0112] In this method, the amino group of tyrosine or a derivative thereof is reacted with an acyl halide of the formula ZC(O)Hal, where Hal is a halogen, preferably Cl or Br, or an acyl anhydride of the formula ZC(O)OC(O)Z to acylate the amino nitrogen atom. Typically, the reaction conditions are dilute base in a suitable solvent, for example bicarbonate in water/THF. Other suitable mild bases and solvents/solvent mixtures will be readily apparent to those having ordinary skill in organic synthesis. Numerous starting tyrosine derivatives are commercially available or can be readily synthesized using standard chemical reactions. An example of the synthesis of a compound within the scope of the invention is shown below. 12

[0113] In this scheme, R may be any suitable group which is non-reactive under the reaction conditions. Examples of suitable R groups include substituted and unsubstituted alkyl, alkenyl, aryl, arylalkyl, etc. Additional compounds of the invention can then be prepared by acylating the phenyl hydroxy group with an activated carbonyl followed by the formation of a carbamate, carbonate or thiocarbamate as described below.

[0114] Solid phase reaction chemistry provides a convenient method for synthesizing the compounds of the invention. FMOC- or BOC-protected amino acids and derivatives thereof are readily available and can be used as starting materials in the synthesis of the compounds of the invention. The protected amino acid is initially attached to a synthetic resin having an available coupling group, such as an available hydroxy (e.g. benzyloxy resin beads). Coupling is achieved using known chemical reactions, e.g. condensation reactions using for example DIPC or DMAP, to attach the amino acid to the solid support. Any known coupling reactions and resin surfaces may be used. The amino nitrogen is then deprotected using, for example, a weak base such as piperidine or other suitable base. The free amino group can then be reacted with an activated ester such a HBTU or HOBT ester of a suitable benzoic acid to form the desired A group. The resulting hydroxy compounds are within the scope of the invention.

[0115] Additional compounds can be prepared by further reacting the hydroxy group to form esters, carbamates, carbonates, etc. using known chemistry. For example, the hydroxy compounds can be reacted with a carbonyl synthon such as phosgene, carbonyldiimidazole or p-nitrophenylformate followed by a primary or secondary amine, including cyclic amines, to form carbamates as shown in the reaction scheme below. 13

[0116] In this scheme, a=DIPC cat./DMAP; b=20% piperidine/DMA or DMF; c=a substituted benzoic acid/HBTU or other amide coupling agent/TEA or other weak base; d=primary or secondary amine; e=TFA/triethylsilane, for example.

[0117] Compounds of structure I were typically synthesized manually via solid phase synthesis on p-alkoxybenzyl alcohol resin (Advanced Chemtech, USA) as shown above. Commercially available FMOC protected tyrosine or other tyrosine analogs (X2/X3) were purchased from BACHEM Ca., Advanced ChemTech U.S.A., or Calbiochem Corp. (Ca.). Typically 1 mmol of FMOC-tyrosine (or tyrosine analog) was added to 1 g of p-alkoxybenzylalcohol resin in 50 mL dichloromethane. Diisopropylcarbodiimide (DIPC, 1 mmol) was added followed by catalytic dimethylaminopyridine (DMAP, 0.1 mmol) and the resulting mixture was stirred under nitrogen at 20 C for 4 hours. The resin was then washed with dicloromethane and dimethylacetamide (DMA) and the FMOC group was removed via mixing with 20% piperidine in DMA for fifteen minutes. The resin was then washed three times with DMA to remove excess piperidine.

[0118] Ortho-Chlorobenzoic acid (2 mmol) or other substituted benzoic acid was mixed with HBTU (2 mmol) or other suitable activating agent in 20 mL of DMA and added the previously washed resin. N-methylmorpholine or triethylamine (4 mmol) was added and the mixture was sparged with nitrogen for 30 minutes. The resin was washed with dichloromethane and treated with 2 mmol of p-nitrophenylchloroformate (phosgene or carbonyldiimidazole can also be used) and 0.05 mmol DMAP in 20 mL of DMA for 1 h. Excess reagents were washed away and 2 mmol of morpholine or other substituted amine RaRb-NH in 20 mL dichloromethane was added. The mixture was sparged overnight at room temperature and washed with dichlormethane.

[0119] Treatment with TFA containing 5% triethylsilane for 1 hour afforded the crude product. The crude material was extracted from the resin by stirring with 100 mL of 2:1H2O/CH3CN for 5 minutes followed by filtration to remove the resin. The crude filtrate was lyophilized and purified by preparative reverse phase C18 HPLC (CH3CN/H2O gradient, 0.1% TFA) to afford purified material. Pure fractions (>98% pure by analytical HPLC) were characterized by electrospray ionization mass spectrometry (Sciex API100) and proton NMR, lyophilized to dryness and resuspended in DMSO at 10 mM just prior to biological assay. Serial dilutions of peptide starting at 0.5 mM were titrated into an ELISA format assay and the IC50 for each compound was determined.

[0120] II. Solution Phase Synthesis:

[0121] Alternatively, inhibitors with general structure I can be synthesized in three steps via solution phase chemistry starting with commercially available (L)-tyrosine or tyrosine analogs having substituents at X2/X3 and/or Y. A general synthesis of type I Analogs is depicted below. This type of synthesis is amenable to scale up and for introducing ester prodrugs.

[0122] Typically, 100 mmols of (L)-tyrosine or similar tyrosine analog is dissolved in 500 mL THF/H2O (1:1) and 300 mmols of sodium bicarbonate is added followed by 110 mmols (1.1 eq.) of a suitable benzoyl chloride or anhydride of general structure Z-COCl. The solution is stirred at room temperature for 1 h. The mixture is concentrated via rotary evaporation and acidified to pH<3 with 1 N HCL. The acidified solution is extracted with ethyl acetate and the organic layer is washed with satd. NaCl and evaporated to dryness. Crystallization of the crude material from ethylacetate/hexane affords pure compound as determined by analytical HPLC (average yield; 75 mmol or 75%).

[0123] If a suitable benzoyl chloride or anhydride is not available then the corresponding substituted benzoic acid (100 mmols) is used in combination with HBTU or other amide coupling reagent. If this route is employed, 100 mmols of (L)-tyrosine or similar tyrosine analog is dissolved in 250 mL of dimethylformamide. In a separate vessel, the appropriate benzoic acid (110 mmols) in DMF is mixed with 110 mmols of HBTU or other amide coupling agent and 300 mmols of triethylamine or other weak base (NMM, DIPEA etc.). The mixture is allowed to stand for 10 minutes and then added to the tyrosine in one portion. After stirring for 1 hour at room temperature, the reaction mixture is concentrated under high vacuum and resuspended in ethyl acetate. The suspension is washed with 1 N HCL, water and satd. NaCl and evaporated to dryness. Crystallization affords pure compound (average yield; 66 mmol or 66%).

[0124] Purified 2 (50 mmols) is dissolved in 400 mL of THF and 100 mmols of TEA (or other base) is added followed by 50 mmol of p-nitrophenylchloroformate (phosgene or carbonyldiimidazole can also be used). The reaction is stirred for 1 hour at room temp, filtered and the filtrate is concentrated to dryness to afford crude compound which can be isolated via crystallization from ethyacetate/hexane or used directly in the next step. If phosgene or CDI is used instead of p-nitrophenylchloroformate then isolation at this stage is not an option and an appropriate amine RaRb-NH is added to the above reaction 30 minutes after the addition of p-nitrophenylchloroformate.

[0125] The p-nitrophenylcarbonate (10 mmol is dissolved in 100 ml of THF, 15 mmol of an appropriate amine RaRb-NH is added, and the reaction is stirred overnight at room temp. The solvent is evaporated and the resulting residue is tritrated with hexane to remove byproducts. Crystallization affords the desired O-carbamoyl-N-acyltyrosine inhibitor. 14

[0126] Synthesis of Biphenylalanine Compounds (II).

[0127] The biphenyl compounds of structure II can be synthesized starting from substituted or unsubstituted halo phenylalanine compounds as shown below. The protected amino acid starting material can be coupled to a resin as described above or using any known resin/coupling reaction system known in the art. The biphenyl ring system can then be prepared by reacting the halo amino acid with a substituted or unsubstituted phenyl boronic acid. If desired, a substituent on one of the phenyl rings may then be further elaborated using known chemical reactions. For example, a substituent containing a nitrogen atom can be further modified to provide amides, carbamates, etc. A substituent having a hydroxy or carboxy group can be converted to an ester, carbonate, etc. 15

[0128] A representative synthetic procedure for preparing the compounds of the invention is set forth below and refers to the scheme shown above.

[0129] Halo-N-FMOC-Phe 1 (10 mmol) is suspended in 80 mL of DCM along with Wang resin (8 mmol) in a peptide synthesis flask with bubbling N2 gas providing agitation. DIPC is added to a 0.25 M concentration followed by DMAP (1 mmol) and the reaction bubbled for 16 h. After washing the resin (3×80 mL with alternating DMF, methanol, and DCM) the resin is treated with 80 mL of 25% piperidine in NMP for 1 h followed by another wash cycle. A solution of 0.25 M 2-Chlorobenzoic acid, HOBT, HBTU, and DIPEA in NMP is stirred for 0.5 h prior to addition to the resin 2. The reaction bubbled for 16 h and is washed as before. 100 mg portions of the resin can then be transferred to reaction vessels on the Argonaut Quest 210 parallel synthesis instrument and suspended with 0.25 M boronic acid and DIPEA in 3 mL of degassed NMP that contained Pd(PPh3)2Cl2 catalyst. The reactions were stirred magnetically and heated to 80 degrees C. for 16 h. The resin 3 was washed (3×5 mL with alternating DMF, methanol, and DCM). When in the previous step a formyl substituted boronic acid was used, the resin was swelled with 0.5 M amine in 2 mL of 2% AcOH/NMP. After 1 h of stirring a 2 mL portion of 0.5 M Na(OAc)3BH in NMP was added, followed by agitation for 16 h. After washing the resin 3 or 4 as before, 2 mL of TFA that contained 5% DCM and 2% triethylsilane was added followed by 1 h of agitation and filtration. The resin was washed with 1 mL of DCM and combined with the original filtrate. The reductive amination and TFA deblocking were also performed in polypropylene 48 well reaction blocks. The TFA was evaporated either by a vacuum centrifuge or via a stream of nitrogen gas to yield 20 mg of crude oil containing products 5 that were purified on HPLC and confirmed by Electrospray mass spectroscopy.

[0130] In cases where primary amines were used, compounds 4 can be further elaborated using standard methods to prepare sulfonamides 6, amides and carbamates 7, and disubstituted amines 8 as shown below. 16

[0131] The free alpha carboxylic acid may be converted to an ester or to an amide using reactions well known in the art. For example, a free carboxyl group can be reacted with a suitable alcohol in the presence of an acid to esterify the carboxyl group using well known reactions and reagents. Similarly, amides are formed by reacting the carboxylic acid with an amine with removal of the water produced by the condensation using known methods. A example of a reaction for esterification is shown below. 17

[0132] Also included in the scope of this invention are prodrugs of the compounds described above. Suitable prodrugs include known amino-protecting and carboxy-protecting groups which are released, for example hydrolyzed, to yield the parent compound under physiologic conditions. A preferred class of prodrugs are compounds in which a nitrogen atom in an amino, amidino, aminoalkyleneamino, iminoalkyleneamino or guanidino group is substituted with a hydroxy (OH) group, an alkylcarbonyl (—CO—W) group, an alkoxycarbonyl (—CO—OW), an acyloxyalkyl-alkoxycarbonyl (—CO—O—W—O—CO—W) group where W is a monovalent or divalent group and as defined above or a group having the formula —C(O)—O—CP1P2-haloalkyl, where P1 and P2 are the same or different and are H, lower alkyl, lower alkoxy, cyano, halo lower alkyl or aryl. Preferably the nitrogen atom is one of the nitrogen atoms of the amidino group of the compounds of the invention. These prodrug compounds are prepared reacting the compounds of the invention described above with an activated acyl compound to bond a nitrogen atom in the compound of the invention to the carbonyl of the activated acyl compound. Suitable activated carbonyl compounds contain a good leaving group bonded to the carbonyl carbon and include acyl halides, acyl amines, acyl pyridinium salts, acyl alkoxides, in particular acyl phenoxides such as p-nitrophenoxy acyl, dinitrophenoxy acyl, fluorophenoxy acyl, and defluorophenoxy acyl. The reactions are generally exothermic and are carried out in inert solvents at reduced temperatures such as −78 to about 50 C. The reactions are usually also carried out in the presence of an inorganic base such as potassium carbonate or sodium bicarbonate, or an organic base such as an amine, including pyridine, triethylamine, etc. One manner of preparing prodrugs is described in U.S. Ser. No. 08/843,369 filed Apr. 15, 1997 the contents of which are incorporated herein by reference in their entirety.

EXAMPLES

[0133] The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention. All patent and literature citations are herein incorporated by reference in their entirety.

[0134] Specific and representative compounds have been prepared and assayed for inhibitory binding activity using the methods described above and are shown in table 1 below. In the assay results, A represents an IC50 value greater than 1.0 micromolar and B represents an IC50 value less than 1.0 micromolar.

TABLE 1
Structure	Compound number	Assay Result
18	001	B
19	002	B
20	003	B
21	004	B
22	005	B
23	006	B
24	007	B
25	008	B
26	009	B
27	010	B
28	011	B
29	012	B
30	013	B
31	014	B
32	015	B
33	016	B
34	017	B
35	018	B
36	019	B
37	020	B
38	021	B
39	022	B
40	023	B
41	024	B
42	025	B
43	026	B
44	027	B
45	028	B
46	029	B
47	030	B
48	031	B
49	032	B
50	033	B
51	034	B
52	035	B
53	036	B
54	037	B
55	038	B
56	039	B
57	040	B
58	041	B
59	042	B
60	043	A
61	044	A
62	045	A
63	046	A
64	047	A
65	048	A
66	049	A
67	050	A
68	051	A
69	052	A
70	053	A
71	054	A
72	055	A
73	056	A
74	057	A
75	058	B
76	059	B
77	060	B
78	061	B
79	062	B
80	063	B
81	064	B
82	065	B
83	066	B
84	067	B
85	068	B
86	069	B
87	070	B
88	071	B
89	072	B
90	073	B
91	074	B
92	075	B
93	076	B
94	077	B
95	078	B
96	079	B
97	080	B
98	081	B
99	082	B
100	083	B
101	084	B
102	085	B
103	086	B
104	087	B
105	088	B
106	089	B
107	090	B
108	091	B
109	092	B
110	093	B
111	094	B
112	095	B
113	096	B
114	097	B
115	098	B
116	099	B
117	100	B
118	101	B
119	102	B
120	103	B
121	104	A
122	105	A
123	106	A
124	107	A
125	108	A
126	109	A
127	111	B
128	112	B
129	113	B
130	114	B
131	115	B
132	116	A
133	117	B
134	118	B
135	119	B
136	120	B
137	121	B
138	122	B
139	123	B
140	124	B
141	125	B
142	126	B
143	127	B
144	128	B

[0135] The following table 2 illustrates further compounds prepared and assayed, each of which was found to inhibit binding activity exhibiting an IC50 value less than 1.0 micromolar using the methods described above.

TABLE 2
145
R	R'-compdno.	R	R'-compdno.
	129		130
146	147	148	149
	131		132
150	151	152	153
	133		134
154	155	156	157
	135		136
158	159	160	161
	137		138
162	163	164	165
	139		140
166	167	168	169
	141		142
170	171	172	173
	143		144
174	175	176	177
	145		146
178	179	180	181
	147		148
182	183	184	185
	149		150
186	187	188	189
	151		152
190	191	192	193
	153		154
194	195	196	197
	155		156
198	199	200	201
	157		158
202	203	204	205
	159		160
206	207	208	209
	161		162
210	211	212	213
	163		164
214	215	216	217
	165		166
218	219	220	221
	167		168
222	223	224	225
	169		170
226	227	228	229
	171		172
230	231	232	233
	173		174
234	235	236	237
	175		176
238	239	240	241
	177		178
242	243	244	245
	179		180
246	247	248	249
	181		182
250	251	252	253
	183		184
254	255	256	257
185		186
258	259	260	261
	187		188
262	263	264	265
	189		190
266	267	268	269
	191		192
270	271	272	273
	193		194
274	275	276	277
	195		196
278	279	280	281
	197		198
282	283	284	285
	199		200
286	287	288	289
	201		202
290	291	292	293
	203		204
294	295	296	297
	205		206
298	299	300	301
	207		208
302	303	304	305
	209		210
306	307	308	309
	211		212
310	311	312	313
	213		214
314	315	316	317
	215		216
318	319	320	321
	217		218
322	323	324	325
	219		220
326	327	328	329
	221		222
330	331	332	333
	223		224
334	335	336	337
	225		226
338	339	340	341
	227		228
342	343	344	345
	229		230
346	347	348	349
	231		232
350	351	352	353
	233		234
354	355	356	357
	235		236
358	359	360	361
	237		238
362	363	364	365
	239		240
366	367	368	369
	241		242
370	371	372	373
	243		244
374	375	376	377
	245		246
378	379	380	381
	247		248
382	383	384	385
	249		250
386	387	388	389
	251		252
390	391	392	393
	253		254
394	395	396	397
	255		256
398	399	400	401
	257		258
402	403	404	405
	259		260
406	407	408	409
	261		262
410	411	412	413
	263		264
414	415	416	417
	265		266
418	419	420	421
	267		268
422	423	424	425
	269		270
426	427	428	429
	271		272
430	431	432	433
	273		274
434	435	436	437
	275		276
438	439	440	441
	277		278
442	443	444	445
	279		280
446	447	448	449
	281		282
450	451	452	453
	283		284
454	455	456	457
	285		286
458	459	460	461
	287		288
462	463	464	465
	289		290
466	467	468	469
	291		292
470	471	472	473
	293		294
474	475	476	477
	295		296
478	479	480	481
	297		298
482	483	484	485
	299		300
486	487	488	489
	301		302
490	491	492	493
	303		304
494	495	496	497
	305		306
498	499	500	501
	307		308
502	503	504	505
	309		310
506	507	508	509
	311		312
510	511	512	513
	313		314
514	515	516	517
	315		316
518	519	520	521
	317		318
522	523	524	525
	319		320
526	527	528	529
	321		322
530	531	532	533
	323		324
534	535	536	537
	325		326
538	539	540	get,0333
	327		328
541	542	543	544
	329		330
545	546	547	548
	331		332
549	550	551	552
	333		334
553	554	555	556
	335		336
557	558	559	560
	337		338
561	562	563	564
	339		340
565	566	567	568
	341		342
569	570	571	572
	343		344
573	574	575	576
	345		346
577	578	579	580
	347		348
581	582	583	584
	349		350
585	586	587	588
	351		352
589	590	591	592
	353		354
593	594	595	596
	355		356
597	598	599	600
	357		358
601	602	603	604
	359		360
605	606	607	608
	361		362
609	610	611	612
	363		364
613	614	615	616
	365		366
617	618	619	620
	367		368
621	622	623	624
	369		370
625	626	627	628
	371		372
629	630	631	632
	373		374
633	634	635	636
	375		376
637	638	639	640
	377		378
641	642	643	644
	379		380
645	646	647	648
	381		382
649	650	651	652
	383		384
653	654	655	656
	385		386
657	658	659	660
	387		388
661	662	663	664
	389		390
665	666	667	668
	391		392
669	670	671	672

[0136] The following table illustrates further compounds prepared and assayed, each of which was found to inhibit binding activity exhibiting an IC50 value greater than 1.0 micromolar using the methods described above.

673
R	R’	R	R’
674	675	676	677
678	679	680	681
682	683	684	685
686	687	688	689
690	691	692	693
694	695	696	697
698	699	700	701
702	703	704	705
706	707	708	709
710	711	712	713
714	715	716	717
718	719	720	721

Claims

1. A compound of the formula I, II or III:

2. A compound according to claim 1, having the formula:

3. The compound of claim 2 having structure I.

4. The compound of claim 2, having structure II.

5. The compound of one of claims 2, wherein X1 X2, X3 are each independently H, alkyl, alkenyl, alkynyl, aryl, arylalkyl, heterocylyl, or heteroaryl.

6. The compound of claim 5, wherein X1 is C(O)OR, C(O)R, or C(O)SR.

7. The compound of claim 5, wherein X1 is C(O)NRaRb.

8. The compound of claim 5, wherein X1 is C(O)NRaRb and wherein Ra and Rb together with the nitrogen to which they are attached form a 5-membered or 6-membered heterocyclyl or heteroaryl group substituted with 0-4 substituents R.

9. The compound of claim 7, wherein X1 is a member selected from the group consisting of

10. The compound of claim 9, wherein X1 is

11. The compound of claim 7, wherein X1 is C(O)NRaRb and wherein Ra and Rb are independently hydrogen, substituted or unsubstituted alkyl, aryl, heterocyclyl, or heteroaryl.

12. The compound of claim 11, wherein X1 is a member selected from the group consisting of

13. The compound of claim 11, wherein R1, R5 or both are not hydrogen.

14. The compound of claim 1, wherein X2, X3, Z or a combination thereof are hydrogen.

15. The compound of claim 1, wherein A is selected from the group consisting of

16. The compound of claim 1, wherein A is

17. The compound of claim 1, wherein X2 is a member selected from the group consisting of

18. The compound of claim 1, wherein the compound has S stereochemical configuration.

19. A composition, comprising the compound of claim 1 and a carrier or excipient.

20. A medicament, comprising the compound of claim 1 and a therapeutically inert carrier or excipient.

21. A medicament for treating a disease or condition associated with binding of alpha4beta7 to MAdCAM-1 or alpha4beta1 to VCAM-1, comprising the compound of claim 1 and a therapeutically inert carrier or excipient.

22. A medicament for treating rheumatoid arthritis, asthma, psoriasis, multiple sclerosis, inflammatory bowel disease, ulcerative colitis, pouchitis, Crohn's disease, Celiac disease, nontropical Sprue, graft-versus-host disease, pancreatitis, insulin-dependent diabetes mellitus, mastitis, cholecystitis, pericholangitis, chronic sinusitis, chronic bronchitis, pneumonitis, collagen disease, eczema or systemic lupus erythematosis, comprising the compound of claim 1 and a therapeutically inert carrier or excipient.

23. A method for treating a disease or condition associated with binding of alpha4beta7 to MAdCAM-1 or alpha4beta1 to VCAM-1, comprising administering an effective amount of the compound of claim 1 to a mammal in need thereof.

24. A method for treating rheumatoid arthritis, asthma, psoriasis, multiple sclerosis, inflammatory bowel disease, ulcerative colitis, pouchitis, Crohn's disease, Celiac disease, nontropical Sprue, graft-versus-host disease, pancreatitis, insulin-dependent diabetes mellitus, mastitis, cholecystitis, pericholangitis, chronic sinusitis, chronic bronchitis, pneumonitis, collagen disease, eczema or systemic lupus erythematosis, comprising administering an effective amount of the compound of claim 1 to a mammal in need thereof.