Claims
- 1. An isolated nucleic acid comprising a nucleotide sequence encoding a pub polypeptide or fragment thereof, or a nucleotide sequence complementary thereto, said pub polypeptide including an amino acid sequence at least 80% identical to the amino acid sequence designated by one or more of SEQ ID No. 2 and SEQ ID No. 6, which pub polypeptide or fragment thereof possesses a ubiquitin ligase activity.
- 2. The nucleic acid of claim 1, wherein said pub polypeptide possesses a calcium binding motif.
- 3. The nucleic acid of claim 1, wherein said pub polypeptide ubiquitinates cdc25.
- 4. The nucleic acid of claim 1, wherein said pub polypeptide encoding nucleotide sequence is at least 90% identical to the amino acid sequence designated by SEQ ID No. 2.
- 5. The nucleic acid of claim 1, wherein said pub polypeptide encoding nucleotide sequence is at least 90% identical to the amino acid sequence designated by SEQ ID No. 6.
- 6. The nucleic acid of claim 1, wherein said pub polypeptide is a fusion protein.
- 7. The nucleic acid of claim 1, which nucleic acid hybridizes under stringent conditions to a nucleic acid probe having a sequence represented by at least 60 consecutive nucleotides of SEQ ID No. 1, or a sequence complementary thereto.
- 8. The nucleic acid of claim 1, wherein said pub polypeptide encoding nucleotide sequence hybridizes under stringent conditions to a nucleic acid probe having a sequence represented by at least 60 consecutive nucleotides of SEQ ID No. 1 or 5, or a sequence complementary thereto.
- 9. The nucleic acid of claim 1, further comprising a transcriptional regulatory sequence operably linked to said nucleotide sequence so as to render said nucleic acid suitable for use as an expression vector.
- 10. An expression vector, capable of replicating in at least one of a prokaryotic cell and eukaryotic cell, comprising the nucleic acid of claim 9.
- 11. A host cell transfected with the expression vector of claim 10 and expressing said recombinant polypeptide.
- 12. A method of producing a recombinant pub polypeptide comprising culturing the cell of claim 11 in a cell culture medium to express said recombinant polypeptide and isolating said recombinant polypeptide from said cell culture.
Parent Case Info
This application is a continuation and claims priority to U.S. Ser. No. 08/539,205, filed Oct. 4, 1995 now U.S. Pat. No. 6,001,619.
FUNDING
Work described herein was supported by National Institutes of Health Grant No. GM39620 and the Howard Hughes Medical Institute.
Work described herein was supported by National Institutes of Health Grant and the Howard Hughes Medical Institute. The United States Government has certain rights in the invention.
Non-Patent Literature Citations (6)
Entry |
J.T. Mulligan et al. GenBank Accession No. L11119. Feb. 17, 1993.* |
J.M. Huibregtse et al. “A Family of Proteins Structurally and Functionally Related to the E6-AP Ubiquitin-Protein Ligase”, PNAS 92: 2563-2567. 3/1995.* |
GenBank Accession No. R19498. Apr. 14, 1995.* |
GenBank Accession No. T74302. Mar. 7, 1995.* |
GenBank Accession No. R14620. Apr. 13, 1995.* |
GenBank Accession No. R61402. May 24, 1995. |
Continuations (1)
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Number |
Date |
Country |
Parent |
08/539205 |
Oct 1995 |
US |
Child |
09/392163 |
|
US |