Claims
- 1. A substantially pure polypeptide that (1) catalyzes the covalent attachment of ubiquitin to E2A, and (2) has a sequence that is at least 70% identical to SEQ ID NO.:2.
- 2. The polypeptide of claim 1, wherein said polypeptide has the sequence of a mammalian UBCE2A.
- 3. The polypeptide of claim 1, wherein the sequence of said polypeptide comprises SEQ ID NO.:2.
- 4. An isolated DNA molecule encoding the polypeptide of claim 1.
- 5. The DNA molecule of claim 4, wherein said polypeptide has the sequence of a mammalian UBCE2A.
- 6. The DNA molecule of claim 4, wherein the sequence of said polypeptide comprises SEQ ID NO.:2.
- 7. The DNA molecule of claim 5, wherein said DNA molecule hybridizes to a probe consisting of a sequence that is complementary to the coding sequence of SEQ ID NO.:1 when hybridized and washed under the following stringency conditions: 55° C., 0.1×SSC, 0.1% SDS.
- 8. A vector comprising the DNA molecule of claim 4.
- 9. A cell comprising the DNA molecule of claim 4.
- 10. A method of making a polypeptide, said method comprising:
(a) culturing the cell of claim 9 under conditions permitting expression of said polypeptide from said DNA, and (b) harvesting said polypeptide from said cell or from the medium surrounding said cell.
- 11. An antibody that specifically binds a mammalian UBCE2A.
- 12. A method of inhibiting the proliferation of a cell, said method comprising:
(a) identifying an animal having a cell the proliferation of which is susceptible to being inhibited by increasing the level of the transcription factor E2A in the cell; and (b) introducing into the cell a proteasome inhibitor.
- 13. A method of inhibiting the proliferation of a cell, said method comprising:
(a) identifying an animal having a cell the proliferation of which is susceptible to being inhibited by increasing the level of the transcription factor E2A in the cell; and (b) introducing into the cell a mutant E2A that possesses the transcription factor activity of wild type E2A but that lacks (i) the UBCE2A binding site of wild-type E2A, or (ii) at least one of the lysine residues which are ubiquitination sites on wild-type E2A.
- 14. A method of inhibiting the proliferation of a cell that expresses the transcription factor E2A, said method comprising introducing into the cell a compound that reduces the level of UBCE2A biological activity in the cell.
- 15. The method of claim 14, wherein said compound is an anti-UBCE2A antibody.
- 16. The method of claim 14, wherein said compound is a single-stranded nucleic acid at least 12 nucleotides in length that is antisense to at least a portion of the coding strand of said cell's naturally-occurring gene or MRNA encoding UBCE2A.
- 17. An isolated, single-stranded DNA molecule at least 12 nucleotides in length which is antisense to at least a portion of the coding strand of a naturally-occurring gene or MRNA encoding UBCE2A.
- 18. An isolated molecule of DNA that is transcribed into an MRNA that: (1) is approximately 1.1, 1.5, or 2.1 kilobases in length; and (2) hybridizes to a DNA probe consisting of a sequence that is complementary to (a) the coding sequence of SEQ ID NO.:1, or (b) a naturally occurring MRNA encoding human UBCE2A, when hybridized and washed under the following conditions: 55° C., 0.1×SSC, 0.1% SDS.
- 19. A substantially pure polypeptide encoded by the DNA of claim 18.
- 20. A substantially pure polypeptide consisting of a mutant form of the mammalian transcription factor E2A that differs from wild type E2A in that it (1) is unable to bind UBCE2A, and so cannot be ubiquitinated by UBCE2A; or (2) lacks one or more of the lysine residues that are ubiquitination sites on wild type E2A.
- 21. A DNA molecule comprising SEQ ID NO:6.
Parent Case Info
[0001] This application claims benefit from U.S. Ser. No. 60/014,388, filed Mar. 28, 1996, and U.S. Ser. No. 08/825,476, filed Mar. 28, 1997.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60014388 |
Mar 1996 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
08986605 |
Dec 1997 |
US |
Child |
09785671 |
Feb 2001 |
US |
Parent |
08825476 |
Mar 1997 |
US |
Child |
08986605 |
Dec 1997 |
US |