ULTRASENSITIVE CHEMILUMINESCENT ENZYME LINKED ASSAYS

Information

  • Research Project
  • 2147544
  • ApplicationId
    2147544
  • Core Project Number
    R44DK047727
  • Full Project Number
    2R44DK047727-02
  • Serial Number
    47727
  • FOA Number
  • Sub Project Id
  • Project Start Date
    3/1/1994 - 30 years ago
  • Project End Date
    8/31/1997 - 27 years ago
  • Program Officer Name
  • Budget Start Date
    9/30/1995 - 29 years ago
  • Budget End Date
    8/31/1996 - 28 years ago
  • Fiscal Year
    1995
  • Support Year
    2
  • Suffix
  • Award Notice Date
    9/29/1995 - 29 years ago
Organizations

ULTRASENSITIVE CHEMILUMINESCENT ENZYME LINKED ASSAYS

DESCRIPTION: (Adapted from the applicant's abstract) The objective of the proposed research is to develop ultrasensitive chemiluminescent assays for HIV-1 antibodies by Western blotting and immunoblotting assays for cystic fibrosis. The applicant has developed a proprietary dual-enzyme- label chemiluminescent detection system combining the actions of alkaline phosphatase (AP) and horse radish peroxidase (HRP). AP hydrolyzes a substrate to produce a luminescence enhancer for generating chemiluminescence from a new proprietary substrate for HRP. The applicant will label each member of a pair of probes with AP or HRP. Binding of both probes by an analyte on a membrane, as in Western and immunoblotting assays, places the enzymes in close proximity, facilitating chemiluminescence enhancement. In addition, the applicant will develop sets of chemiluminescent reagents for these assays. The proposed research should provide a new generation of chemiluminescent tests with improved sensitivity and specificity over colorimetric and other chemiluminescent tests. Technology developed will be applicable to research kits for performing chemiluminescent assays for proteins and nucleic acids.

IC Name
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
  • Activity
    R44
  • Administering IC
    DK
  • Application Type
    2
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    847
  • Ed Inst. Type
  • Funding ICs
  • Funding Mechanism
  • Study Section
    ZRG7
  • Study Section Name
  • Organization Name
    LUMIGEN, INC.
  • Organization Department
  • Organization DUNS
    193979002
  • Organization City
    SOUTHFIELD
  • Organization State
    MI
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    480344302
  • Organization District
    UNITED STATES