Claims
- 1. A protein solubilization reagent, comprising:
a dry urea-agent in a container; a diluent for re-hydration of the dry urea-agent; and an instruction for combining a pre-determined volume of the diluent with a predetermined amount of the dry urea-agent.
- 2. The protein solubilization reagent according to claim 1, wherein the dry urea-agent comprises of urea and one or more agents selected from a group consisting of dry thiourea, a detergent, a sufobetaine, a sulfonate, a buffering agent, and a dye.
- 3. The protein solubilization reagent according to claim 1, wherein the diluent comprises of water and one or more agents selected from a group consisting of a dye, a glycerol, a detergent, a carrier ampholyte, and a buffering agent.
- 4. The protein solubilization reagent according to claim 1, wherein approximately 1-2 ml of the diluent is mixed with approximately per one gram dry weight of the dry urea-agent to achieve urea concentration approximately 6-9M in the re-hydrated buffer.
- 5. The protein solubilization reagent according to claim 1, wherein approximately 1-2 ml of the diluent is mixed with approximately per one gram dry weight of the dry urea-agent to achieve urea concentration approximately 6-8M and/or thiourea concentration approximately 2M in the re-hydrated buffer.
- 6. The protein solubilization reagent according to claim 1, wherein the container for the dry urea-agent is a capsule.
- 7. The protein solubilization reagent according to claim 6, wherein the capsule is an open-able capsule.
- 8. The protein solubilization reagent according to claim 1, wherein the container for the dry urea-agent is a sachet.
- 9. The protein solubilization reagent according to claim 1, wherein the dry urea-agent each 100 gram urea is mixed with 41-43 gram of thiourea.
- 10. The protein solubilization reagent according to claim 1, wherein the dry urea-agent each 100 gram urea is mixed with 41-43 gram of thiourea and 5-6 gm of an agent selected from a group consisting of a detergent, a sufobetaine, and a sulfonate.
- 11. The protein solubilization reagent according to claim 1, wherein the dry urea-agent each 100 gram urea is mixed with 41-43 gram of thiourea and 10-12 gm of an agent selected from a group consisting of a detergent, a sufobetaine, and a sulfonate.
- 12. The protein solubilization reagent according to claim 1, wherein the dry urea-agent is provided in tablet form.
- 13. A protein solubilization reagent, for treatment of protein, comprising:
a dry urea-agent in a capsule, wherein the urea-agent consists of urea and one or more agents selected from a group consisting of dry thiourea, a detergent, a sufobetaine, a sulfonate, a buffering agent, and a dye.
- 14. A protein solubilization reagent, for treatment of protein, comprising:
a dry urea-agent in a container, wherein the urea-agent comprises of urea and one or more agents selected from a group consisting of dry thiourea, a detergent, a sufobetaine, a sulfonate, a buffering agent, and a dye; and an instruction for re-hydration of the urea-agent to achieve urea concentration 6-9 molar and/or thiourea concentration approximately 2 molar.
- 15. The protein solubilization reagent according to claim 14, wherein the container for the dry urea-agent is a capsule.
- 16. The protein solubilization reagent according to claim 15, wherein the capsule is an open-able capsule.
- 17. The protein solubilization reagent according to claim 15, wherein the capsule is packaged with a means to maintain humidity at or below ambient.
- 18. The protein solubilization reagent according to claim 14, wherein the dry urea-agent each 100 gram urea is mixed with 41-43 gram of thiourea.
- 19. The protein solubilization reagent according to claim 1, wherein the dry urea-agent each 100 gram urea is mixed with 41-43 gram of thiourea and 5-6 gm of an agent selected from a group consisting of a detergent, a sufobetaine, and a sulfonate.
- 20. The protein solubilization reagent according to claim 1, wherein the dry urea-agent each 100 gram urea is mixed with 41-43 gram of thiourea and 10-12 gm of an agent selected from a group consisting of a detergent, a sufobetaine, and a sulfonate.
RELATED PATENT APPLICATION
[0001] This patent application claims priority from pending provisional patent application Serial No. 60/313,387, filing date Aug. 17, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60313387 |
Aug 2001 |
US |