Claims
- 1. A uricase derived from a thermophilic Bacillus sp. TD-90 (FERM BP-795) microorganism and characterised as follows:
- (1) action: oxidatively decomposes uric acid;
- (2) optimum pH for enzyme activity: pH from 5 to 10;
- (3) the effect of pH on stability; at a pH from 5 to 9, activity is maintained during storage for 15 days at 30.degree. C.;
- (4) optimum temperature for enzyme activity is 45.degree. to 50.degree. C.;
- (5) the effect of temperature on stability: no inactivation of the enzyme was observed after 10 minutes when the temperature was 50.degree. C. or below therefor original activity was not lost;
- (6) molecular weight: about 120,000.
Priority Claims (1)
Number |
Date |
Country |
Kind |
60-120399 |
Jun 1985 |
JPX |
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Parent Case Info
This is a division of application Ser. No. 06/866,572 filed May 22, 1986.
US Referenced Citations (2)
Number |
Name |
Date |
Kind |
3810820 |
Laboureur et al. |
May 1974 |
|
4753882 |
Takashio et al. |
Jun 1988 |
|
Non-Patent Literature Citations (2)
Entry |
Kaltwasser, H., J. Bacteriology, vol. 104, pp. 780-786, (1971). |
Analytical Biochemistry, 38, 65-84, (1970), J. L. Mahler, "A New Bacterial Uricase for Uric Acid Determination". |
Divisions (1)
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Number |
Date |
Country |
Parent |
866572 |
May 1986 |
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