Patent Application
20230218606
The invention is based on and claims the benefit of priority from Chinese application No. 202010078808.7, filed on Feb. 3, 2020, the disclosure of which are incorporated herein by reference in its entirety.
The present application relates to use of hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof, and a pharmaceutical composition comprising the above-mentioned compound in the treatment of a SARS-CoV-2 infection.
Hydroxychloroquine (compound of Formula I) has a chemical name of 2-[[4-[(7-chloro quinolin-4-yl)amino]pentyl]ethylamino]-ethanol; chloroquine (compound of Formula II) has a chemical name of N′,N′-Diethyl-N-4-(7-chloroquinolin-4-yl)-1,4-pentanediamine.
Both the two compounds are 4-aminoquinoline antimalarials, which have functions such as immunosuppression, anti-inflammation, reduction of ultraviolet-induced reaction, sun protection, anti-thrombosis, influence on porphyrin metabolism, anti-hyperlipidemia, anti-proliferation, and anti-pathogen microorganisms. Currently, hydroxychloroquine sulfate is clinically used for the treatment of rheumatoid arthritis, juvenile chronic arthritis, discoid and systemic lupus erythematosus, and skin lesions caused or exacerbated by sunlight. Chloroquine is clinically used to treat falciparum malaria, vivax malaria and quartan malaria that are sensitive to chloroquine, it can also be used for suppressive prevention of malaria symptoms, and it can also be used for the treatment of extraintestinal amebiasis, connective tissue disease, photosensitivity diseases (e.g., erythema solare), etc.
Hydroxychloroquine was artificially synthesized in 1944 and was initially used for antimalarial treatment. In 1955, it was used for the treatment of systemic lupus erythematosus (SLE). Compared with chloroquine phosphate, because hydroxychloroquine has a hydroxyl group, it retains the original efficacy of chloroquine, and has a toxicity half lower than that of chloroquine, thereby having better safety.
Studies have found that in addition to the above known clinical effects, hydroxychloroquine exhibits activities against flavivirus and dengue virus in vitro.
The IC50 of hydroxychloroquine for DENV-2 infected cells is as follows: 10.1±1.6 µM (A549 cells); 12.9±4.2 µM (Hepa1-6 cells); 12.9±1.9 µM (WS-1 cells). In the A549 cell line, hydroxychloroquine at 50 to 80 µM can reduce DENV-2 virus titer by 100 times. In addition, hydroxychloroquine can inhibit the DENV-2 to infect J774A.1 macrophages (IC50 = 9.7 ± 1.3 µM).
Studies have found that hydroxychloroquine is an inhibitor of Zika virus protease NS2B-NS3 and has an inhibitory constant Ki of 92.34±11.91 µM for Zika virus protease NS2B-NS3. Hydroxychloroquine has the effect of resisting ZIKV infection in pregnant mice, the dosage of which is 40 mg/kg/day, and which is intraperitoneally administrated from the first day after ZIKV infection. The results indicate that compared to the mice treated with PBS, the placenta of mice treated with hydroxychloroquine has a lower level of ZIKV infection.
The 2019 novel coronavirus (2019-nCoV) is a new coronavirus strain that has never been found in humans before. On Feb. 11, 2020, the International Committee on Taxonomy Viruses (ICTV) announced that the official name of 2019 novel Coronavirus (2019-nCoV) is called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). On the same day, the World Health Organization (WHO) announced that the official name of the disease caused by this virus is COVID-19. The symptoms of SARS-CoV-2 virus infection are mainly pneumonia, and can be divided into simple infection, mild pneumonia, severe pneumonia, acute respiratory distress syndrome, sepsis, septic shock and so on according to the severity of disease. Patients with simple infection may have nonspecific symptoms, such as fever, cough, sore throat, nasal congestion, fatigue, headache, muscle pain or discomfort, and the elderly and immunosuppressed people may have atypical symptoms. Patients with mild pneumonia mainly have cough, dyspnea and polypnea. Severe pneumonia can be seen in adolescents, adults or children, and the main symptoms of which include increased breathing frequency, severe respiratory failure or dyspnea, central cyanosis, drowsiness, unconsciousness or convulsion, gasp, etc. The lung images of acute respiratory distress syndrome are bilateral ground glass shadows, which cannot be completely explained by effusion, lobar exudation or atelectasis or lung mass shadows, and the main symptom of which is pulmonary edema. Patients with sepsis often have fatal organ dysfunction, and the most critical patients are those with septic shock, and they may have a high probability of death.
At present, the 2019 novel coronavirus (SARS-CoV-2) infection is mainly treated with supportive therapy in clinic, and no specific antiviral drug is available.
The purpose of the present application is to discover a drug with an antiviral activity against a coronavirus, especially SARS-CoV-2, which can be used for the treatment of a relative disease caused by infection thereof, for example, simple infection (such as fever, cough and sore throat), pneumonia, acute respiratory infection or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock.
Through creative research in the present application, it is found that chloroquine phosphate has a good protective effect on SARS-CoV-2 infected cells in vitro, with EC50=1.13 µM, CC50>100 µM, SI>88.5; the compound of Formula I hydroxychloroquine has a toxicity of only half that of chloroquine phosphate in human body, has a function of inhibiting the replication of SARS-CoV-2, and has a good therapeutic effect in the treatment of diseases caused by SARS-CoV-2.
The present application provides a compound having a structure represented by Formula I or Formula II, a geometric isomer, a pharmaceutically acceptable salt and/or a solvate or a hydrate thereof:
According to the present application, the pharmaceutically acceptable salts of the compound of the present application include an inorganic or organic acid salt thereof and an inorganic or organic base salt thereof. The present application relates to all forms of the above salts, including but not limited to: sodium salt, potassium salt, calcium salt, lithium salt, meglumine salt, hydrochloride salt, hydrobromide salt, hydroiodide salt, nitrate salt, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, acetate, propionate, butyrate, oxalate, pivalate, adipate, alginate, lactate, citrate, tartrate, succinate, maleate, fumarate, picrate, aspartate, gluconate, benzoate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate and embonate and so on.
According to the present application, hydroxychloroquine represented by Formula I or chloroquine represented by Formula II can inhibit virus replication in a cell and reduce viral nucleic acid load in a cell culture.
After creative invention and research, the inventors of the present application have discovered some new features of hydroxychloroquine represented by Formula I or chloroquine represented by Formula II:
hydroxychloroquine represented by Formula I or chloroquine represented by Formula II can reduce the viral nucleic acid load at micromolar concentration level in SARS-CoV-2 infected cells.
The present application also relates to a pharmaceutical composition comprising hydroxychloroquine represented by Formula I and/or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a pharmaceutically acceptable solvate or hydrate thereof, and a pharmaceutically acceptable carrier. The pharmaceutical composition can be administered through various routes, such as oral tablet, capsule, powder, oral liquid, injection and transdermal preparation. According to conventional pharmaceutical practices, the pharmaceutically acceptable carrier includes diluent, filler, disintegrant, wetting agent, lubricant, coloring agent, flavoring agent or other conventional additives. The typical pharmaceutically acceptable carrier includes, for example, microcrystalline cellulose, starch, crospovidone, povidone, polyvinylpyrrolidone, maltitol, citric acid, sodium lauryl sulfonate or magnesium stearate, etc.
Another aspect of the present application relates to a pharmaceutical composition, which comprises the compound of the present application and at least one pharmaceutically acceptable carrier. The pharmaceutical composition can be prepared into various forms according to different administration routes.
The present application also relates to use of hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof in the manufacture of a medicament for the treatment of a disease or an infection caused by a SARS-CoV-2 (e.g., a respiratory disease (including but not limited to simple infection (such as fever, cough and sore throat), pneumonia, acute or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome), sepsis and septic shock, etc.),
The present application also relates to use of hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof in the manufacture of a medicament as a SARS-CoV-2 inhibitor, or in the manufacture of a medicament for inhibiting the replication or reproduction of SARS-CoV-2 in a cell (e.g., a cell of mammal),
The present application also relates to use of a pharmaceutical composition in the manufacture of a medicament as a SARS-CoV-2 inhibitor, or in the manufacture of a medicament for inhibiting the replication or reproduction of SARS-CoV-2 in a cell (e.g., a cell of mammal), wherein the pharmaceutical composition comprises hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof,
preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient. Specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present application also relates to use of a pharmaceutical composition in the manufacture of a medicament for the treatment of a disease or an infection caused by a SARS-CoV-2 (e.g., a respiratory disease (including but not limited to simple infection (such as fever, cough and sore throat), pneumonia, acute respiratory infection or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, etc.)), wherein the pharmaceutical composition comprises hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof,
preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient. Specifically, the pharmaceutical composition is a solid preparation, an injection, an external preparation, a spray, a liquid preparation, or a compound preparation.
The present application also relates to a method for treating and/or preventing a disease or a viral infection in a mammal in need thereof, or a method for inhibiting the replication or reproduction of SARS-CoV-2 in a mammal in need thereof, wherein the method comprises administering to the mammal in need thereof a therapeutically and/or prophylactically effective amount of hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrates thereof, or a therapeutically and/or prophylactically effective amount of a pharmaceutical composition comprising hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof,
wherein, the disease includes a disease caused by a SARS-CoV-2 (e.g., a respiratory disease (including but not limited to simple infection (such as fever, cough and sore throat), etc., pneumonia, acute respiratory infection or severe acute respiratory infection (SARI), hypoxic respiratory failure and acute respiratory distress syndrome, sepsis and septic shock, etc.)), the viral infection includes an infection caused by a SARS-CoV-2.
The present application also relates to hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof, or a pharmaceutical composition comprising hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof, for use as a SARS-CoV-2 inhibitor, or for use in inhibiting the replication or reproduction of SARS-CoV-2 in a cell (e.g., a cell in mammal),
preferably, the pharmaceutical composition also comprises a pharmaceutically acceptable carrier or excipient.
The present application also relates to hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or hydrate thereof, or a pharmaceutical composition comprising hydroxychloroquine represented by Formula I or chloroquine represented by Formula II, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof, for use in the treatment of a disease or an infection caused by a SARS-CoV-2 (e.g., a respiratory disease (including but not limited to simple infection (such as fever, cough and sore throat), pneumonia, acute respiratory infection or severe acute respiratory infection, hypoxic respiratory failure and acute respiratory distress syndrome), sepsis and septic shock, etc.),
preferably, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier or excipient.
In some embodiments, the pharmaceutically acceptable salt of hydroxychloroquine represented by Formula I or chloroquine represented by Formula II includes an inorganic or organic acid salt thereof and an inorganic or organic base salt thereof,
the pharmaceutically acceptable salt includes, but is not limited to: sodium salt, potassium salt, calcium salt, lithium salt, meglumine salt, hydrochloride salt, hydrobromide salt, hydroiodide salt, nitrate salt, sulfate, hydrogen sulfate, phosphate, hydrogen phosphate, acetate, propionate, butyrate, oxalate, pivalate, adipate, alginate, lactate, citrate, tartrate, succinate, maleate, fumarate, picrate, aspartate, gluconate, benzoate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate and embonate and so on.
In some embodiments, the pharmaceutically acceptable salt of chloroquine of Formula II is chloroquine sulfate.
In some embodiments, the pharmaceutically acceptable salt of chloroquine of Formula II is chloroquine phosphate.
In some embodiments, the disease caused by a SARS-CoV-2 described in the present application is COVID-19.
In the present application, the official name of the term “2019 novel coronavirus (2019-nCoV)” is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
In the present application, the official name of the term “disease caused by 2019 novel coronavirus (2019-nCoV)” is COVID-19.
The pharmaceutical composition described in the present application can be administered through various routes, such as oral tablet, capsule, powder, oral liquid, injection and transdermal preparation. According to conventional pharmaceutical practices, the pharmaceutically acceptable carrier includes diluent, filler, disintegrant, wetting agent, lubricant, coloring agent, flavoring agent or other conventional additives. The typical pharmaceutically acceptable carrier includes, for example, microcrystalline cellulose, starch, crospovidone, povidone, polyvinylpyrrolidone, maltitol, citric acid, sodium lauryl sulfonate or magnesium stearate, etc.
In some embodiments, the mammal includes bovine, equine, caprid, suidae, canine, feline, rodent, primate, wherein the preferred mammal is a human.
The pharmaceutical composition described in the present application can be prepared into various forms according to different administration routes.
According to the present application, the pharmaceutical composition can be administered in any one of the following routes: oral administration, spray inhalation, rectal administration, nasal administration, buccal administration, vaginal administration, topical administration, parenteral administration such as subcutaneous, intravenous, intramuscular, intraperitoneal, intrathecal, intraventricular, intrasternal and intracranial injection or infusion, or administration with the help of an explant reservoir. Among them, oral, intraperitoneal or intravenous administration is preferred.
When orally administered, the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof can be prepared into any form of orally acceptable preparation, including but not limited to a tablet, a capsule, an aqueous solution or an aqueous suspension. Generally, the carrier for use in a tablet includes lactose and corn starch, and a lubricant such as magnesium stearate can also be added. The diluent for use in a capsule generally includes lactose and dry corn starch. The aqueous suspension is usually used by mixing an active ingredient with a suitable emulsifier and a suitable suspending agent. If necessary, a sweetener, a flavoring agent or a coloring agent can also be added to the above-mentioned forms of oral preparation.
When rectally administered, the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate, and/or a hydrate thereof can generally be prepared in a form of suppository, which is prepared by mixing the drug with a suitable non-irritating excipient. The excipient is present in solid state at room temperature, but melts at the rectal temperature to release the drug. Such excipient includes cocoa butter, beeswax and polyethylene glycol.
When topically administered, especially for the treatment of easily accessible affected-surface or organ, such as eye, skin, or lower intestinal neurological disease by topical application, the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof can be prepared in various forms of topical preparations according to different affected-surfaces or organs, the specific instructions are as follows:
When topically administered to eye, the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof can be formulated into a preparation form such as micronized suspension or solution, the carrier used is isotonic sterile saline with a certain pH, and a preservative such as benzyl chloride alkoxide may or may not be added. In addition, for administration to eye, the compound can also be prepared in a form of ointment such as vaseline ointment.
When topically administered to skin, the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salts, a solvate and/or a hydrate thereof can be prepared into a suitable form such as an ointment, a lotion or a cream, in which the active ingredient is suspended or dissolved in one or more carriers. The carrier for use in an ointment includes, but is not limited to mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyethylene oxide, polypropylene oxide, emulsifying wax, and water. The carrier for use in a lotion or a cream includes, but is not limited to mineral oil, sorbitan monostearate, Tween-60, cetyl ester wax, hexadecenyl aryl alcohol, 2-octyldodecanol, benzyl alcohol and water.
When topically administered to lower intestinal tract, the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof can be prepared into a form such as rectal suppository as described above or a suitable enema preparation form, in addition, a topical transdermal patch can also be used.
The chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof can also be administered in a preparation form of sterile injection, including sterile injectable aqueous solution or oil suspension, or sterile injectable solutions, wherein the usable carrier and solvent includes water, Ringer’s solution and isotonic sodium chloride solution. In addition, a sterilized non-volatile oil such as monoglyceride or diglyceride can also be used as solvent or suspension media.
The drugs of the above various preparation forms can be prepared according to conventional methods in the pharmaceutical field.
In the present application, the term “therapeutically effective amount” or “prophylactically effective amount” refers to an amount that is sufficient to treat or prevent a patient’s disease but is sufficiently low to avoid serious side effects (at a reasonable benefit/risk ratio) within a reasonable medical judgment. The therapeutically effective amount of the compound will change according to the factors such as the selected specific compound (e.g., considering the efficacy, effectiveness, and half-life of compound), the selected administration route, the treated disease, the severity of the treated disease, the patient’s age, size, weight and physical disease, medical history, duration of treatment, nature of concurrent therapy, desired therapeutic effect, etc., but can still be routinely determined by those skilled in the art.
In addition, it should be noted that the specific dosage and method of using the chloroquine or hydroxychloroquine, a geometric isomer, a pharmaceutically acceptable salt, a solvate and/or a hydrate thereof for different patients depends on many factors, including the patient’s age, weight, gender, natural health status, nutritional status, active strength of drug, administration time, metabolic rate, severity of disease, and subjective judgment of physician. Herein it is preferred to use a dosage between 0.001-100 mg/kg body weight/day.
The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.
The technical solutions in the examples of the present application will be described clearly and completely in combination with the drawings in the examples of the present application. Obviously, the described examples are only a part of the examples of the present application, rather than all the examples. The following description of at least one exemplary example is actually only illustrative, and is not intended to limit the present application and its application or use. On the basis of the examples in the present application, all other examples obtained by those skilled in the art without creative work shall fall within the protection scope of the present application.
When the specific techniques or conditions are not indicated in the Examples, the Examples are carried out according to the techniques or conditions described in the literature in the field or according to the product specifications. The materials or equipment used herein, the manufacturers of which are not indicated, are the conventional products that are commercially available.
Vero E6 cells (purchased from ATCC, Catalog No. 1586) was inoculated on a 24-well plate, cultured for 24 hours; then virus infection was carried out. Specifically, SARS-CoV-2 (2019-nCoV) virus (nCoV-2019BetaCoV/Wuhan/WIV04/2019 strain, preserved by Wuhan Institute of Virology, Chinese Academy of Sciences) was diluted with 2% cell maintenance solution (formulation: FBS (purchased from Gibco company, Catalog No. 16000044) was added to MEM (purchased from Gibco, Catalog No. 10370021) at a volume ratio of 2%, thereby obtaining the 2% cell maintenance solution) to a corresponding concentration, and then added to a 24-well plate so that each well contained a viral load of 1OOTCID50. Next, chloroquine phosphate and hydroxychloroquine (chloroquine phosphate was purchased from Sigma-Aldrich, Catalog No. C6628; hydroxychloroquine was purchased from MCE company, Catalog No. HY-B1370) were diluted with 2% cell maintenance solution to corresponding concentrations and added separately to the corresponding wells, so that the final concentrations of the drugs were 50 µM, 16.67 µM, 5.56 µM, 1.85 µM, 0.62 µM, 0.21 µM, 0.068 µM, respectively, then the plate was placed in a 37° C., 5% CO2 incubator and cultured for 48 hours. To the vehicle control group, the 2% cell maintenance solution without any test drugs was added.
RNA extraction kit was purchased from Qiagen Company, Catalog No. 74106. The consumables (spin columns, RNase-free 2ml collection tubes, etc.) and reagents (RLT, RW1, RPE, RNase-free water, etc.) involved in the following RNA extraction steps were part of the kit. The following extraction steps were recommended steps in the kit instruction.
In the experiment, the reverse transcription kit (PrimeScript™ RT reagent Kit with gDNA Eraser, Catalog No. RR047Q) produced by TaKaRa was used for RNA reverse transcription. The steps were as follows.
Fluorescence quantitative PCR was used to detect the number of copies per milliliter of the original virus solution.
The reaction system was mixed by using TB Green Premix (Takara, Cat#RR820A), and the amplification reaction and reading were carried out with StepOne Plus Real-time PCR instrument (brand: ABI). The copy number contained in per milliliter of the original virus solution was calculated. The steps were as follows:
The cytotoxicity test of drugs was carried out by using CCK-8 kit (Beyotime). Specific steps were as follows:
The results of the virus proliferation inhibition experiment showed that the test compound chloroquine phosphate at concentrations of 50 µM, 16.67 µM, and 5.56 µM could effectively inhibit the replication of SARS-CoV-2 virus genome in the infected supernatant.
The results of cytotoxicity test (see:
According to calculations, the half-maximal effective concentration (EC50) of chloroquine phosphate was 1.13 µM, the half-cytotoxic concentration (CC50) to cells was greater than 100 µM, and the selectivity index (SI) was greater than 88.5. The results showed that chloroquine phosphate could effectively block SARS-CoV-2 virus infection at low micromolar concentrations, and showed a higher SI.
Vero E6 cells were inoculated into a 24-well plate, cultured for 24 hours, and then subjected to virus infection. Four groups of different infection doses were set, which are MOI of 0.01, MOI of 0.02, MOI of 0.2 and MOI of 0.8, respectively. SARS-CoV-2 (2019-nCoV) viruses were diluted with 2% cell maintenance solution to the corresponding concentration, and then added to a 24-well plate so that the cell viral load in each well reached the set infection dose. Then, chloroquine phosphate and hydroxychloroquine were separately diluted with 2% cell maintenance solution to reach corresponding concentrations, and added to the corresponding wells so that the final concentrations of the drugs were 50 µM, 16.67 µM, 5.56 µM, 1.85 µM, 0.62 µM, 0.21 µM, 0.068 µM, respectively, then they were placed in a 37° C., 5% CO2 incubator and cultured for 48 hours. To the cell control group, 2% cell maintenance solution without any test drugs was added.
RNA extraction was carried out by referring to the method as described in step (2) in Example 1.
RNA reverse transcription was carried out by referring to the method as described in step (3) in Example 1.
With reference to the method as described in step (4) in Example 1, fluorescence quantitative PCR was used to detect the number of copies per milliliter of the original virus solution.
Cytotoxicity test of drugs to cells was carried out by referring to the method as described in step (5) in Example 1.
The results of cytotoxicity test (see: A in
The results of the virus proliferation inhibition experiment showed that the test compounds can effectively inhibit the replication of SARS-CoV-2 viral genome in the infection supernatant at the MOI of 0.01, 0.02, 0.2 and 0.8 (see: B to E in
The results of immunofluorescence analysis (IFA) and confocal micrograph analysis (see:
Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application rather than to limit them; although the present application has been described in detail with reference to the preferred examples, those of ordinary skill in the art should understand that the specific implementation of the present application may be modified or some technical features may be equivalently replaced without departing from the spirit of the technical solutions of the present application, and all of them shall be covered by the scope of the technical solution sought to be protected by the present application.
| Number | Date | Country | Kind |
|---|---|---|---|
| 202010078808.7 | Feb 2020 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/CN2020/093989 | 6/2/2020 | WO |
