Patent Application
20230364169
The invention relates to a second use of Antrodia cinnamomea, and more particularly to a use of Antrodia cinnamomea for increasing alcohol metabolism or/and hangover.
Taiwanofungus camphoratus, with a commonly name of Antrodia cinnamomea, also known as Antrodia camphoratus, cinnamomum fungus, fungus grown in a cavity, and godsent fungus, is a medicinal fungus belonging to the Fomitopsidaceae Antrodia, and only native to Taiwan. The medicinal physiological activity of Antrodia cinnamomea is very extensive and significant. At least 78 species have been isolated and identified, of which 39 are triterpenes, and 31 of which have confirmed their chemical structures. The chemical compounds contained in Antrodia cinnamomea can be roughly classified into polysaccharides, triterpenes and sterols, benzenoids, benzoquinone derivatives, maleic acid and succinic acid derivatives; and according to research, Antrodia cinnamomea has a variety of efficacies on subject health promotion, such as antibacterial, antiviral, antineoplastic, and enhancing organism immunity.
In addition to drinking alcohol as one of the ways for modern people to relieve stress, it is also a culture in social life. Generally speaking, moderate intake of alcohol can relax people's mind, but intaking excessive alcohol will cause discomfort such as dizziness, vomiting, low cognitive ability, nausea, and low mobility, and it may continue to affect the life of the next day. In the long run, it will also have adverse effects on health. At present, there are many products on the market that claim to have an efficacy of dispelling the effects of alcohol, for example, by intaking fructose to increase alcohol metabolism. However, using fructose to increase alcohol metabolic rate will not only increase the content of uric acid and lactic acid, but also increase the risk of suffering from metabolic syndrome.
A main object of the invention is to provide a secondary use of Antrodia cinnamomea, which is capable of reducing a content of alcohol and/or acetaldehyde in blood, promoting alcohol metabolism, and achieving efficacies of dispelling the effects of alcohol preventively and therapeutically.
Another object of the invention is to provide a use of Antrodia cinnamomea for dispelling the effects of alcohol and/or increasing alcohol metabolism, which is not hepatotoxic and is capable of improving the dehydrogenase, acetaldehyde dehydrogenase or/and SOD enzyme activity, in order to achieve efficacies of increasing alcohol metabolism, lowering alcohol concentration in the blood and treating or preventing acute alcoholism or other liver disease caused by an elevated alcohol concentration in blood.
In order to achieve the above-mentioned objects, this invention disclosed a method for enhancing the metabolism of alcohol in a subject comprising administering an effective amount of Antrodia cinnamomea powder or composition thereof to a subject in need of enhancing the metabolism of alcohol. It means that by administering an effective amount of the Antrodia cinnamomea powder to the subject who is in a need of dispelling the effects of alcohol can accelerating metabolism of alcohol and/or acetaldehyde, so that it can prevent or reduce the effects of alcohol or uncomfortable feeling from hangover efficiently.
Furthermore, in one embodiment, the invention disclosed a method for lowering the ethanol concentration in blood of a subject comprising administering an effective amount of Antrodia cinnamomea powder or composition thereof to a subject in need of reducing an adverse effect caused by an elevated alcohol concentration in blood, whereby the administration of Antrodia cinnamomea powder or composition thereof for lowering the ethanol concentration in blood.
According to the Antrodia cinnamomea powder or composition thereof has dual efficacies of dispelling the effects of alcohol preventively and therapeutically, which means that when the subject takes the composition for dispelling the effects of alcohol before drinking alcohol, the composition for dispelling the effects of alcohol has an efficacy of dispelling the effects of alcohol preventively; and when the subject takes the composition for dispelling the effects of alcohol after drinking alcohol, the composition for dispelling the effects of alcohol exerts an efficacy of dispelling the effects of alcohol therapeutically.
In the other embodiment, the invention disclosed the method for treating or preventing acute alcoholism or liver disease caused by an elevated plasma alcohol level in a subject comprising administering an effective amount of Antrodia cinnamomea powder or composition thereof to a subject who has an elevated plasma alcohol level, whereby the administration of Antrodia cinnamomea powder or composition thereof for increasing alcohol dehydrogenase, acetaldehyde dehydrogenase or SOD enzyme activity.
For example, when an subject intakes excessive alcohol and has symptoms of acute alcoholism, by administering an effective amount of the Antrodia cinnamomea powder disclosed in the invention is capable of metabolizing the alcohol in the subject's body in a short time.
In other words, the Antrodia cinnamomea powder can be used to prepare an alcohol metabolism accelerant or an SOD enzyme activity accelerant. And if the Antrodia cinnamomea powder is prepared to be the alcohol metabolism accelerant, it is capable of achieving efficacies of promoting alcohol metabolism and avoiding accumulation in the body by enhancing an activity of alcohol dehydrogenase and/or acetaldehyde dehydrogenase in the body; and if Antrodia cinnamomea powder is prepared to be SOD enzyme activity accelerant, when an subject takes an effective amount of the Antrodia cinnamomea powder after being intoxicated, an SOD enzyme activity in the body will be increased, which is not only capable of achieving an efficacy of promoting decomposition of alcohol, but also reducing an oxidative stress at the same time, in order to achieve an efficacy of protecting cells.
In the embodiments, the Antrodia cinnamomea powder can be made to a composition, such as a pharmaceutical preparation, a beverage, or a food product. Furthermore, the composition can be an oral or parenteral solution, a syrup, a powder, a capsule, a tablet, a beverage, a food product, or a food supplement.
In another embodiment, the effective amount of the Antrodia cinnamomea powder is at least 79.5 mg/day for an adult.
In the embodiments, the Antrodia cinnamomea powder is produced by crushing and grinding the Antrodia cinnamomea mycelium; for example, the Antrodia cinnamomea powder is made according to following steps of:
The objects, features, and achieved efficacies of the invention can be understood from the description, drawings and tables of the following preferred embodiments, in which:
Intoxication rates of mice described in the following examples are based on whether the mice have lost righting reflex as the standard. Detection steps are as follows: after the mice are fed with alcohol, put them into a cage with their backs facing downward, if the mice keep a posture with their backs facing downward for more than 30 seconds, it is judged that the mice have lost their righting reflex and are intoxicated.
Sample 1: water-soluble Antrodia cinnamomea powder, wherein the water-soluble Antrodia cinnamomea powder is a powdery extract obtained by supercritical carbon dioxide extraction, emulsification and drying of Antrodia cinnamomea mycelium powder, and after testing, it is confirmed that it contains the same content of triterpenoids as wild Antrodia cinnamomea.
For example, the Antrodia cinnamomea mycelium powder is processed with supercritical carbon dioxide extraction and then concentrated to obtain a paste-like supercritical Antrodia cinnamomea mycelium extract, the paste-like supercritical Antrodia cinnamomea mycelium extract is mixed with grease substances in a weight ratio of approximately 1:6-7 at a temperature of 70° C. to obtain a Antrodia cinnamomea oil-phase solution. An aqueous-phase solution is prepared, which contains water, dextrin fibers and sucrose fatty acid esters, and is mixed in a weight ratio of approximately 357-358:72:1. After the Antrodia cinnamomea oil-phase solution and the aqueous-phase solution are processed with a microemulsification procedure, freeze-dried and sieved to obtain a water-soluble Antrodia cinnamomea powder.
Sample 2: Commercially available Hovenia dulcis functional drink, containing 1.3% of Hovenia dulcis extract concentrate.
Sample 3: Commercially available patented Hovenia dulcis extract (Korea Lifetree Biotech Co., Ltd.).
Three groups of mice, with 8 mice in each of the groups, are intragastrically administered with doses of 0.3 mL/20 g, 0.35 mL/20 g, and 0.4 mL/20 g of alcohol solution (58% Kinmen Kaoliang Liquor-sorghum) respectively, in order to observe an amount of alcohol required to cause lost of righting reflex (LOR) in the mice in each of the groups and none of the mice die.
It is found that a few of the mice die when 0.4 mL/20 g of alcohol solution is administered; none of the mice die when 0.35 mL/20 g of alcohol solution is administered, intoxication rate is 100%, and an average intoxication time is 794.0±38.02 minutes; none of the mice die when 0.3 mL/20 g of alcohol solution is administered, and intoxication rate is 25%; therefore, the mice are intragastrically administered with 0.35 mL/20 g of alcohol solution in the subsequent tests of dispelling the effects of alcohol.
The mice are randomly divided into groups, wherein:
The mice in each of the groups are fasted for 12 hours, and then are treated according to the above-mentioned conditions in each of the groups. After 30 minutes, all the mice in each of the groups except the first group are intragastrically administered with 0.35 mL/20 g of 58% Kinmen Kaoliang Liquor by body weight, intoxication time (meaning the time from being conscious to lost of righting reflex) of the mice in each of the groups is observed and recorded in order to calculate and obtain intoxication rates within 24 hours, wherein whether the mice are intoxicated or not is based on whether the mice have lost righting reflex as the standard.
It is found that the intoxication rates of the mice in each of the groups are 100%, and none of the mice die. From the results in
Although the results in
The mice in each of the groups are fasted for 12 hours as described in Example 3, and all the mice are intragastrically administered with 0.35 mL/20 g of 58% Kinmen Kaoliang Liquor by body weight. After 30 minutes, the first group is administered with an equal volume of physiological saline, the mice in the second group to the seventh group are administered in a one-time administration; then sober time (meaning the time from lost of righting reflex to being conscious) of the mice in each of the groups is observed, and 0, 60, 120, 240 and 360 minutes after being fed with alcohol, blood is collected to determine contents of alcohol and acetaldehyde in the blood, the results are shown in
In this experiment, alcohol solution (0.35 mL/20 g) is administered first, and then drug is administered. Intoxication rates of each of the groups are 100%, and none of the mice die. From the results in
From the results in
It can be known from the above results that the mice disclosed in this example are indeed acute alcoholic mice after being fed with alcohol, and administration of the water-soluble Antrodia cinnamomea powder disclosed in the invention is capable of effectively increasing an efficiency of dispelling the effects of alcohol and shortening the time required for the mice to be sobered from alcohol, and as an administered dose increases, the time required for dispelling the effects of alcohol is shortened; blood test results more clearly confirm that the water-soluble Antrodia cinnamomea powder disclosed in the invention is capable of metabolizing alcohol and acetaldehyde in blood, and reducing alcohol and acetaldehyde contents in blood.
A test method of this example is basically the same as that of example 3, except that the mice are randomly divided into four groups, each of the groups with mice, wherein;
Wherein the Antrodia cinnamomea mycelium powder is obtained by grinding Antrodia cinnamomea mycelium.
Except for the first group, the mice in each of the groups are intragastrically administered with 0.35 mL/20 g of 58% Kinmen Kaoliang Liquor by body weight, and intoxication time (meaning the time from being conscious to lost of righting reflex) of the mice in each of the groups is observed and recorded in order to calculate and obtain intoxication rates within 24 hours.
It can be known from the results that intoxication rates of the mice in each of the groups are 100%, and none of the mice die; an average intoxication time of the mice in the first group is 853.67±9.61 minutes; average intoxication time of the mice in the second group and the third group is 638.38±37.42 minutes and 458.25±55.89 minutes respectively; and an average intoxication time of the mice in the fourth group is 453.75±34.19 minutes.
The above results show that the Antrodia cinnamomea mycelium powder disclosed in the invention does have an efficacy of dispelling the effects of alcohol preventively, and when it reaches twice the dose, its efficacy of dispelling the effects of alcohol preventively is similar to that of the drug silymarin.
A test method of this example is basically the same as that of example 4, and grouping of the mice and their treatment conditions are the same as those described in example 5. 30 minutes after alcohol solution is administered, the mice are administered in a one-time administration separately. Time required for being sobered is recorded, and blood is collected at 0, 60, 120, 240 and 360 minutes after alcohol is fed to determine alcohol and acetaldehyde contents in the blood, the results are shown in Table 3 and Table 4 below.
It can be known from the experimental results that an average intoxication time of the mice in the first group is 745.83±18.17 minutes; average intoxication time of the mice in the second group and the third group is 547.13±32.35 minutes and 340.63±34.45 minutes respectively; and an average intoxication time of the mice in the fourth group is 328.38±35.52 minutes.
It can be known that administration of the Antrodia cinnamomea mycelium powder disclosed in the invention after being fed with alcohol is capable of reducing the intoxication time, which shows that the Antrodia cinnamomea mycelium powder disclosed in the invention does have an efficacy of dispelling the effects of alcohol therapeutically; and from the results in Table 3 and Table 4, it can be known that the Antrodia cinnamomea mycelium powder disclosed in the invention is capable of reducing alcohol and acetaldehyde contents in blood, wherein when twice the dose is administered, an efficacy of the Antrodia cinnamomea mycelium powder disclosed in the invention of reducing alcohol and acetaldehyde contents in blood is better than that of administration of the drug silymarin.
The mice are randomly divided into four groups, 12 in each of the groups, wherein:
After the mice in each of the groups are treated with the above conditions for 4 weeks, except for the normal group, each of the groups is administered with 0.35 ml of alcohol/mouse (10 ml/kg+50% alcohol volume daily converted based on surface areas of human body and mouse body), the alcohol group is administered with 0.35 ml of water/mouse half an hour after being fed with alcohol, and the Antrodia cinnamomea high-dose group and the Antrodia cinnamomea low-dose group are respectively administered with 0.35 ml of the Antrodia cinnamomea mycelium powder/mouse half an hour after being fed with alcohol. After the experiments are done, liver form and conditions, body weight, and liver index of the mice in each of the groups are observed, as shown in Table 5 and
Serum and liver biochemical values of the mice in each of the groups are tested, and the results are shown in Table 6. From the results in Table 6, it can be known that triglycerides, GOT and GPT of the mice in the alcohol group have increased, and administration of the Antrodia cinnamomea mycelium powder disclosed in the invention is capable of reducing triglycerides, GOT and GPT, and an effect of high dose is better, even better than an effect of the drug silymarin. Furthermore, alcohol dehydrogenase and acetaldehyde dehydrogenase activities of the mice in the alcohol group have decreased, and administration of the Antrodia cinnamomea mycelium powder disclosed in the invention is capable of increasing the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, but administration of silymarin does not have an efficacy of increasing the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase.
From the results in Table 7 and Table 8, it can be known that alcohol and acetaldehyde contents of the mice in the alcohol group are 1.15±0.01 mg/dL and 6.80±0.37 mU/mL respectively when being fed with alcohol solution at 120 minutes, which have increased in comparing with the mice in the normal group, but after continuously taking the Antrodia cinnamomea powder disclosed in the invention for 30 days, both the alcohol and acetaldehyde contents decrease. At 120 minutes, alcohol contents of the Antrodia cinnamomea powder low-dose group and the Antrodia cinnamomea mycelium powder high-dose group are 1.03±0.03 mg/dL and 0.81±0.04 mg/dL respectively, and acetaldehyde contents of the Antrodia cinnamomea powder low-dose group and the Antrodia cinnamomea mycelium powder high-dose group are 4.45±0.6 mU/mL and 3.49±0.48 mU/mL respectively. At 360 minutes, alcohol contents of the Antrodia cinnamomea mycelium powder low-dose group and the Antrodia cinnamomea powder high-dose group are 0.58±0.03 mg/dL and 0.37±0.05 mg/dL respectively, and acetaldehyde contents of the Antrodia cinnamomea mycelium powder low-dose group and the Antrodia cinnamomea powder high-dose group are 3.30±0.30 mU/mL and 2.30±0.83 mU/mL respectively; the above results show that the Antrodia cinnamomea mycelium powder disclosed in the invention is capable of strengthening alcohol metabolism, and with high doses, its alcohol metabolism effect is better, with an effect better than that of silymarin.
Referring to
15 subjects are divided into three groups after taking alcoholic beverages orally. The first group does not take any products for dispelling the effects of alcohol, and the second group and the third group take the water-soluble Antrodia cinnamomea powder and its aqueous-phase solution disclosed in the invention, respectively, wherein a dose is 400 mg; and alcohol concentration in the breath of the subjects in each of the groups is measured at 0, 15, 30, 60, 120 and 180 minutes after being administered. The results are shown in
It can be known from the results in
From the results of the above examples, it can be known that the Antrodia cinnamomea mycelium powder disclosed in the invention does have efficacies of enhancing alcohol metabolism and dispelling the effects of alcohol preventively, and is also capable of improving an activity of SOD enzymes in liver; and the water-soluble Antrodia cinnamomea powder obtained from the supercritically extracted Antrodia cinnamomea mycelium powder disclosed in the invention is capable of achieving the same or even better efficacies of metabolizing alcohol and dispelling the effects of alcohol preventively at a lower dose; and therefore it can be known that the Antrodia cinnamomea mycelium powder and its water-soluble powder disclosed in the invention can be used as effective ingredients in a medical composition for treatment or prevention of intoxication and related liver diseases.
It is to be understood that the above description is only preferred embodiments of the present invention and is not used to limit the present invention, and changes in accordance with the concepts of the present invention may be made without departing from the spirit of the present invention, for example, the equivalent effects produced by various transformations, variations, modifications and applications made to the configurations or arrangements shall still fall within the scope covered by the appended claims of the present invention.
| Number | Date | Country | Kind |
|---|---|---|---|
| 109108997 | Mar 2020 | TW | national |
This application is a Divisional of co-pending application Ser. No. 17/203,145, filed on Mar. 16, 2021, for which priority is claimed under 35 U.S.C. § 120; and this application claims priority of Application No. 109108997 filed in TW on Mar. 18, 2020 under 35 U.S.C. § 119, the entire contents of all of which are hereby incorporated by reference.
| Number | Date | Country | |
|---|---|---|---|
| Parent | 17203145 | Mar 2021 | US |
| Child | 18357668 | US |
