Use of CDK II inhibitors for birth control

Abstract

The subject of this invention is a method for birth control comprising the inhibition of CDK II as well as the use of CDK II inhibitors of general formulas I and II for the development of a pharmaceutical agent for contraception.

Description

This invention relates to a method for birth control, comprising the use of CDK II inhibitors.

For birth control, the broad spectrum of the “standard pill” as a contraceptive agent is available to women, but only the condom or sterilization is approved at this time for men.

Taking the pill regularly leads to ovulation inhibition in women, since it results in suppressing the endogenic steroid hormone production in the ovary by the daily intake of hormones. A drawback of this regular intake consists in that a natural cycle thus is no longer present in women. Moreover, in connection with female patients who are potentially at risk in taking a hormonal preparation, side effects, such as, for example, tender breasts and weight gain, can occur.

There is therefore a need for the development of new reliable agents both for female and for male birth control. In this connection, the infertility that is produced by the substance administration is completely reversible and effective. The infertility should set in relatively quickly and be as long-lasting as possible. Such a contraceptive method should have the fewest possible side effects. To overcome the drawbacks of the known hormonal preparations, these should be non-hormonal preparations in this connection.

The influencing of the maturation of female egg cells (oogenesis) and that of sperm (spermatogenesis) could represent a possible starting point for a common concept for birth control of women and men. Oogenesis and spermatogenesis are processes in whose regulation the enzyme cyclin-dependent kinase II (cyclin-dependent kinase=CDK II) is involved.

CDK 2 is an enzyme kinase whose function in the mitotic and meiotic division of cells is described and that is activated in specific phases of the cell cycle.

For some time, it could be shown that CDK II knock-out mice, which, were unremarkable over a period of up to 2 years and also showed no anatomical characteristics, were completely infertile. This infertility was probably caused by an obvious atrophy of the gonads. Male CDK II^−/− mice did not show any round spermatides in the seminiferous tubules. Postmeiotic cells (spermatocytes, spermatides and spermatozoa) were no longer present. Adult female animals show an atrophic ovary without egg cells and follicles (Ortega et al., Nature Genetics, 2003); Berthet et al.; Current Biology, 2003).

The prior art points out that in the CDK II^−/− animals, a general atrophy and irreversible damage of the gonads result by eliminating the CDK II gene. This strong tissue-destroying effect does not appear to be a very promising basis for creating a preparation for birth control with the inhibition of CDK II.

Consequently, it is not known to date whether an inhibitor of the CDK II could have a specific action on the maturation of germ cells. The achievement of such an effect would presuppose that a potential CDK II inhibitor is able to pass through the cell membrane to prevent the maturation of gametes in the cell by specific CDK II inhibition. At the same time, it would be expected that structurally similar kinases are also inhibited and thus strong side effects occur.

A more selective inhibitor of the CDK II, however, could be used as a pharmaceutical agent for birth control for women and men.

Although the use of CDK II inhibitors for contraception relative to male fertility in a number of applications is disclosed generically in a variety of patent applications (US20030078252, US20020119963, WO02010141, WO02018346, US20030199525), to date any reference to biological action in vitro and in vivo is lacking.

In a surprising way, it was now found that compounds of general formula I in which

• • R¹ stands for hydrogen, halogen, C₁-C₆-alkyl, nitro or for the group —COR⁵, —OCF₃, —(CH₂)_nR⁵, —S—CF₃ or —SO₂CF₃,
  • R² stands for C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl or C₃-C₁₀-cycloalkyl or for a C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl or C₃-C₁₀-cycloalkyl that is substituted in one or more places, in the same way or differently, with hydroxy, halogen, C₁-C₆-alkoxy, C₁-C₆-alkylthio, amino, cyano, C₁-C₆-alkyl, —NH—(CH₂)_n—C₃-C₁₀-cycloalkyl, C₃-C₁₀-cycloalkyl, C₁-C₆-hydroxyalkyl, C₂-C₆-alkenyl, C₂-C₆-alkinyl, C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy-C₁-C₆-alkoxy-C₁-C₆-alkyl, —NHC₁-C₆-alkyl, —N(C₁-C₆-alkyl)₂, —SO(C₁-C₆-alkyl), —SO₂(C₁-C₆-alkyl), C₁-C₆-alkanoyl, —CONR³R⁴, —COR⁵, C₁-C₆-alkylOAc, carboxy, aryl, heteroaryl, —(CH₂)_n-aryl, —(CH₂)_n-heteroaryl, phenyl-(CH₂)_n—R⁵, —(CH₂)_nPO₃(R⁵)₂ or with the group —R⁶ or —NR³R⁴, and the phenyl, C₃-C₁₀-cycloalkyl, aryl, heteroaryl, —(CH₂)_n-aryl and —(CH₂)_n-heteroaryl itself optionally can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy, heteroaryl, benzoxy or with the group —CF₃ or —OCF₃, and the ring of C₃-C₁₀-cycloalkyl and C₁-C₁₀-alkyl optionally can be interrupted by one or more nitrogen, oxygen and/or sulfur atoms, and/or can be interrupted by one or more ═C═O groups in the ring and/or optionally one or more possible double bonds can be contained in the ring, or
  • R² stands for the group
  • X stands for oxygen or for the group —NH—, —N(C₁-C₃-alkyl) or for —OC₃—C₁₀-cycloalkyl, which can be substituted in one or more places, in the same way or differently, with a heteroaromatic compound,
  • or
  • X and R² together form a C₃-C₁₀-cycloalkyl ring, which optionally can contain one or more heteroatoms and optionally can be substituted in one or more places with hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy or halogen,
  • A and B, in each case independently of one another, stand for hydrogen, hydroxy, C₁-C₃-alkyl, C₁-C₆-alkoxy or for the group —SR⁷, —S(O)R⁷, —SO₂R⁷, —NHSO₂R⁷, —CH(OH)R⁷, —CR⁷(OH)—R⁷, C₁-C₆-alkylP(O)OR³OR⁴, —COR⁷ or for
  • A and B together form a C₃-C₁₀-cycloalkyl ring that optionally can be interrupted by one or more nitrogen, oxygen and/or sulfur atoms and/or can be interrupted by one or more ═C═O or ═SO₂ groups in the ring, and/or optionally one or more possible double bonds can be contained in the ring, and the C₃-C₁₀-cycloalkyl ring optionally can be substituted in one or more places, in the same way or differently, with hydroxy, halogen, C₁-C₆-alkoxy, C₁-C₆-alkylthio, amino, cyano, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₁₀-cycloalkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl, —NHC₁-C₆-alkyl, —N(C₁-C₆-alkyl)₂, —SO(C₁-C₆-alkyl), —SO₂R⁷, C₁-C₆-alkanoyl, —CONR³R⁴, —COR⁵, C₁-C₆-alkylOAc, phenyl, or with the group R⁶, whereby the phenyl itself optionally can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy, or with the group —CF₃ or —OCF₃,
  • R³ and R⁴, in each case independently of one another, stand for hydrogen, phenyl, benzyloxy, C₁-C₁₂-alkyl, C₁-C₆-alkoxy, C₂-C₄-alkenyloxy, C₃-C₆-cycloalkyl, hydroxy, hydroxy-C₁-C₆-alkyl, dihydroxy-C₁-C₆-alkyl, heteroaryl, heterocyclo-C₃-C₁₀-alkyl, heteroaryl-C₁-C₃-alkyl, C₃-C₆-cycloalkyl-C₁-C₃-alkyl that optionally is substituted with cyano, or for C₁-C₆-alkyl that optionally is substituted in one or more places, in the same way or differently, with phenyl, pyridyl, phenyloxy, C₃-C₆-cycloalkyl, C₁-C₆-alkyl or C₁-C₆-alkoxy, whereby the phenyl itself can be substituted in one or more places, in the same way or differently, with halogen, C₁-C₆-alkyl, C₁-C₆-alkoxy or with the group —SO₂NR³R⁴, or for the group —(CH₂)_nNR³R⁴, —CNHNH₂ or —NR³R⁴ or
  • R³ and R⁴ together form a C₃-C₁₀-cycloalkyl ring, which optionally can be interrupted by one or more nitrogen, oxygen and/or sulfur atoms, and/or can be interrupted by one or more ═C═O groups in the ring and/or optionally one or more possible double bonds can be contained in the ring,
  • R⁵ stands for hydroxy, phenyl, C₁-C₆-alkyl, C₃-C₆-cycloalkyl, benzoxy, C₁-C₆-alkylthio or C₁-C₆-alkoxy,
  • R⁶ stands for a heteroaryl or a C₃-C₁₀-cycloalkyl ring, whereby the ring has the above-indicated meaning,
  • R⁷ stands for halogen, hydroxy, phenyl, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkinyl, C₃-C₁₀-cycloalkyl with the above-indicated meaning, or for the group —NR³R⁴, or for C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl or C₃-C₁₀-cycloalkyl that is substituted in one or more places, in the same way or differently, with hydroxy, C₁-C₆-alkoxy, halogen, phenyl, —NR³R⁴ or phenyl, which itself can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy, halo-C₁-C₆-alkyl, or halo-C₁-C₆-alkoxy, or R⁷ stands for phenyl, which itself can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C₁-C₆-alkyl or C₁-C₆-alkoxy, halo-C₁-C₆-alkyl, or halo-C₁-C₆-alkoxy,
  • R⁸, R⁹ and
  • R¹⁰, in each case independently of one another, stand for hydrogen, hydroxy, C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl, C₃-C₁₀-cycloalkyl, aryl, heteroaryl or for C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl or C₃-C₁₀-cycloalkyl that is substituted in one or more places, in the same way or differently, with hydroxy, halogen, C₁-C₆-alkoxy, C₁-C₆-alkylthio, amino, cyano, C₁-C₆-alkyl, —NH—(CH₂)_n—C₃-C₁₀-cycloalkyl, C₃-C₁₀-cycloalkyl, C₁-C₆-hydroxyalkyl, C₂-C₆-alkenyl, C₂-C₆-alkinyl, C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy-C₁-C₆-alkoxy-C₁-C₆-alkyl, —NHC₁-C₆-alkyl, —N(C₁-C₆-alkyl)₂, —SO(C₁-C₆-alkyl), —SO₂(C₁-C₆-alkyl), C₁-C₆-alkanoyl, —CONR³R⁴, —COR⁵, C₁-C₆-alkylOAc, carboxy, aryl, heteroaryl, —(CH₂)_n-aryl, —(CH₂)_n-heteroaryl, phenyl-(CH₂)_n—R⁵, —(CH₂)_nPO₃(R⁵)₂ or with the group —R⁶ or —NR³R⁴, and the phenyl, C₃-C₁₀-cycloalkyl, aryl, heteroaryl, —(CH₂)_n-aryl and —(CH₂)_n-heteroaryl itself optionally can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy, or with the group —CF₃ or —OCF₃, and the ring of C₃-C₁₀-cycloalkyl and the C₁-C₁₀-alkyl optionally can be interrupted by one or more nitrogen, oxygen and/or sulfur atoms and/or can be interrupted by one or more ═C═O groups in the ring and/or optionally one or more possible double bonds can be contained in the ring, and
  • n stands for 0-6, as well as the isomers, diastereomers, enantiomers and salts thereof that inhibit the CDK II in vivo and in vitro and thus overcome the drawbacks of the known pharmaceutical agents and can be used for the production of a pharmaceutical agent for contraception.

Alkyl is defined in each case as a straight-chain or branched alkyl radical, such as, for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec.-butyl, tert.-butyl, pentyl, isopentyl, hexyl, heptyl, octyl, nonyl and decyl.

Alkoxy is defined in each case as a straight-chain or branched alkoxy radical, such as, for example, methyloxy, ethyloxy, propyloxy, isopropyloxy, butyloxy, isobutyloxy, sec.-butyloxy, tert.-butyloxy, pentyloxy, isopentyloxy or hexyloxy.

Alkylthio is defined in each case as a straight-chain or branched alkylthio radical, such as, for example, methylthio, ethylthio, propylthio, isopropyithio, butylthio, isobutylthio, sec.-butylthio, tert.-butylthio, pentylthio, isopentylthio or hexylthio.

In general, cycloalkyl is defined as monocyclic alkyl rings such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl or cyclodecyl, but also bicyclic rings or tricyclic rings, such as, for example, norbornyl, adamantanyl, etc.

Ring systems, in which optionally one or more possible double bonds can be contained in the ring, are defined as, for example, cycloalkenyls, such as cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclohexeny, or cycloheptenyl, whereby the linkage can be carried out both to the double bond and to the single bonds.

If A and B, R³ and R⁴, X and R², in each case independently of one another, together form a C₃-C₁₀-cycloalkyl ring, which optionally can be interrupted by one or more heteroatoms such as nitrogen atoms, oxygen atoms and/or sulfur atoms, and/or can be interrupted by one or more ═C═O groups in the ring, and/or optionally one or more possible double bonds can be contained in the ring, however, the above-mentioned definitions are also intended to include the heteroaryl radical or heterocycloalkyl and heterocycloalkenyl.

Halogen is defined in each case as fluorine, chlorine, bromine or iodine.

The alkenyl substituents in each case are straight-chain or branched, whereby, for example, the following radicals are meant: vinyl, propen-1-yl, propen-2-yl, but-1-en-1-yl, but-1-en-2-yl, but-2-en-1-yl, but-2-en-2-yl, 2-methyl-prop-2-en-1-yl, 2-methyl-prop-1-en-1-yl, but-1-en-3-yl, ethinyl, prop-1-in-1-yl, but-1-in-1-yl, but-2-in-1-yl, but-3-en-1-yl, or allyl.

Alkinyl is defined in each case as a straight-chain or branched alkinyl radical that contains 2-6 C atoms, preferably 2-4 C atoms. For example, the following radicals can be mentioned: acetylene, propin-1-yl, propin-3-yl, but-1-in-1-yl, but-1-in-4-yl, but-2-in-1-yl, but-1-in-3-yl, etc.

In each case, the aryl radical comprises 3-12 carbon atoms and can in each case be benzocondensed.

For example, there can be mentioned: cyclopropenyl, cyclopentadienyl, phenyl, tropyl, cyclooctadienyl, indenyl, naphthyl, azulenyl, biphenyl, fluorenyl, anthracenyl, etc.

In each case, the heteroaryl radical comprises 3-16 ring atoms and, instead of carbon, can contain one or more of the same or different heteroatoms, such as oxygen, nitrogen or sulfur in the ring and can be monocyclic, bicyclic or tricyclic, and can in each case in addition be benzocondensed.

For example, there can be mentioned:

Thienyl, furanyl, pyrrolyl, oxazolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl, etc., and benzo derivatives thereof, such as, e.g., benzofuranyl, benzothienyl, benzoxazolyl, benzimidazolyl, indazolyl, indolyl, isoindolyl, etc.; or pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, etc., and benzo derivatives thereof, such as, e.g., quinolyl, isoquinolyl, etc.; or azocinyl, indolizinyl, purinyl, etc., and benzo derivatives thereof; or quinolinyl, isoquinolinyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, naphthyridinyl, pteridinyl, carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, xanthenyl, oxepinyl, etc.

Heterocycloalkyl stands for an alkyl ring that comprises 3-12 carbon atoms, which instead of carbon contains one or more of the same or different heteroatoms, such as, e.g., oxygen, sulfur or nitrogen.

As heterocycloalkyls, e.g., there can be mentioned: oxiranyl, oxethanyl, aziridinyl, azetidinyl, tetrahydrofuranyl, pyrrolidinyl, dioxolanyl, imidazolidinyl, pyrazolidinyl, dioxanyl, piperidinyl, morpholinyl, dithianyl, thiomorpholinyl, piperazinyl, trithianyl, quinuclidinyl, etc.

Heterocycloalkenyl stands for an alkyl ring that comprises 3-12 carbon atoms, which instead of carbon contains one or more of the same or different heteroatoms, such as, e.g., oxygen, sulfur or nitrogen, and which is partially saturated.

As heterocycloalkenyls, e.g., pyran, thiln, dihydroacet, etc., can be mentioned.

If an acid group is included, the physiologically compatible salts of organic and inorganic bases, such as, for example, the readily soluble alkali salts and alkaline-earth salts, as well as N-methyl-glucamine, dimethyl-glucamine, ethyl-glucamine, lysine, 1,6-hexadiamine, ethanolamine, glucosamine, sarcosine, serinol, tris-hydroxy-methyl-amino-methane, aminopropanediol, Sovak base, and 1-amino-2,3,4-butanetriol, are suitable as salts.

If a basic group is included, the physiologically compatible salts of organic and inorganic acids are suitable, such as hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, tartaric acid, i.a.

Those compounds of general formula (I) are especially effective in which

• • R¹ stands for hydrogen, halogen, C₁-C₆-alkyl, nitro or for the group —COR⁵, —OCF₃, —(CH₂)_nR⁵, —S—CF₃ or —SO₂CF₃,
  • R² stands for C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl or C₃-C₁₀-cycloalkyl or for C₁-C₁₀-alkyl, C₂-C₁₀-alkenyl, C₂-C₁₀-alkinyl or C₃-C₁₀-cycloalkyl that is substituted in one or more places, in the same way or differently, with hydroxy, halogen, C₁-C₆-alkoxy, C₁-C₆-alkylthio, amino, cyano, C₁-C₆-alkyl, —NH—(CH₂)_n—C₃-C₁₀-cycloalkyl, C₃-C₁₀-cycloalkyl, C₁-C₆-hydroxyalkyl, C₂-C₆-alkenyl, C₂-C₆-alkinyl, C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy-C₁-C₆-alkoxy-C₁-C₆-alkyl, —NHC₁-C₆-alkyl, —N(C₁-C₆-alkyl)₂, —SO(C₁-C₆-alkyl), —SO₂(C₁-C₆-alkyl), C₁-C₆-alkanoyl, —CONR³R⁴, —COR⁵, C₁-C₆-alkylOAc, carboxy, aryl, heteroaryl, —(CH₂)_n-aryl, —(CH₂)_n-heteroaryl, phenyl-(CH₂)_n-R⁵, —(CH₂)_nPO₃(R⁵)₂ or with the group —R⁶ or —NR³R⁴, and the phenyl, C₃-C₁₀-cycloalkyl, aryl, heteroaryl, —(CH₂)_n-aryl and —(CH₂)_n-heteroaryl itself optionally can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy, heteroaryl, benzoxy or with the group —CF₃ or —OCF₃, and the ring of C₃-C₁₀-cycloalkyl and the C₁-C₁₀-alkyl optionally can be interrupted by one or more nitrogen, oxygen and/or sulfur atoms, and/or can be interrupted by one or more ═C═O groups in the ring and/or optionally one or more possible double bonds can be contained in the ring, or
  • R² stands for the group
  • X stands for oxygen or for the group —NH—, —N(C₁-C₃-alkyl) or for —OC₃—C₁₀-cycloalkyl that can be substituted in one or more places, in the same way or differently, with a heteroaromatic compound,
  • or
  • X and R² together form a C₃-C₁₀-cycloalkyl ring, which optionally can contain one or more heteroatoms and optionally can be substituted in one or more places with hydroxy, C₁-C₆-alkyl, C₁-C₆-alkoxy or halogen,
  • A and B, in each case independently of one another, stand for hydrogen, hydroxy, C₁-C₃-alkyl, C₁-C₆-alkoxy or for the group —S—CH₃, —SO₂—C₂H₄—OH, —CO—CH₃, —S—CHF₂, —S—(CH₂)_n—CH(OH)CH₂N—R³R⁴, —CH₂P(O)OR³OR⁴, —S—CF₃, —SO—CH₃, —SO₂CF₃, —SO₂—(CH₂)_n—N—R³R⁴, —SO₂—NR³R⁴, —SO₂R⁷, —CH—(OH)—CH₃ or for or
  • A and B together can form a group
  • R³ and R⁴, in each case, independently of one another, stand for hydrogen, phenyl, benzyloxy, C₁-C₁₂-alkyl, C₁-C₆-alkoxy, C₂-C₄-alkenyloxy, C₃-C₆-cycloalkyl, hydroxy, hydroxy-C₁-C₆-alkyl, dihydroxy-C₁-C₆-alkyl, heteroaryl, heterocyclo-C₃-C₁₀-alkyl, heteroaryl-C₁-C₃-alkyl, C₃-C₆-cycloalkyl-C₁-C₃-alkyl that optionally is substituted with cyano, or for C₁-C₆-alkyl that optionally is substituted in one or more places, in the same way or differently, with phenyl, pyridyl, phenyloxy, C₃-C₆-cycloalkyl, C₁-C₆-alkyl or C₁-C₆-alkoxy, whereby the phenyl itself can be substituted in one or more places in the same way or differently with halogen, trifluoromethyl, C₁-C₆-alkyl, C₁-C₆-alkoxy or with the group —SO₂NR³R⁴,
    • or for the group —(CH₂)_nNR³R⁴, —CNHNH₂ or —NR³R⁴ or for which optionally can be substituted with C₁-C₆-alkyl,
  • R⁵ stands for hydroxy, phenyl, C₁-C₆-alkyl, C₃-C₆-cycloalkyl, benzoxy, C₁-C₆-alkylthio or C₁-C₆-alkoxy,
  • R⁶ stands for the group
  • R⁷ stands for halogen, hydroxy, phenyl, C₁-C₆-alkyl, —C₂H₄OH, —NR³R⁴, or for the group
  • R⁸, R⁹ and
  • R¹⁰ in each case independently of one another, stand for hydrogen, hydroxy, C₁-C₆-alkyl, C₃-C₆-cycloalkyl or for the group and
  • n stands for 0-6, as well as the isomers, enantiomers, diastereomers and salts thereof.

Those compounds of general formula I, in which

• • R¹ stands for hydrogen, halogen, C₁-C₃-alkyl or for the group —(CH₂)_nR⁵ steht,
  • R² stands for —CH(CH₃)—(CH₂)_n—R⁵, —CH—(CH₂OH)₂, —(CH₂)_nR⁷, —CH(C₃H₇)—(CH₂)_n—R5, —CH(C₂H₅)—(CH₂)_n—R⁵, —CH₂—CN, —CH(CH₃)COCH₃, —CH(CH₃)—C(OH)(CH₃)₂, —CH(CH(OH)CH₃)OCH₃, —CH(C₂H₅)CO—R⁵, C₂-C₄-alkinyl, —(CH₂)_n—COR⁵, —(CH₂)_n—CO—C₁-C₆-alkyl, —(CH₂)_n—C(OH)(CH₃)-phenyl, —CH(CH₃)—C(CH₃)—R⁵, —CH(CH₃)—C(CH₃)(C₂H₅)—R⁵, —CH(OCH₃)—CH₂—R⁵, —CH₂—CH(OH)—R⁵, —CH(OCH₃)—CHR⁵—CH₃, —CH(CH₃)—CH(OH)—CH₂—CH═CH₂, —CH(C₂H₅)—CH(OH)—(CH₂)_n—CH₃, —CH(CH₃)—CH(OH)—(CH₂)_n—CH₃, —CH(CH₃)—CH(OH)—CH(CH₃)₂, (CH₂OAC)₂, —(CH₂)_n—R⁶, —(CH₂)_n—(CF₂)_n—CF₃, —CH(CH₂)_n—R⁵)₂, —CH(CH₃)—CO—NH₂, —CH(CH₂OH)-phenyl, —CH(CH₂OH)—CH(OH)—(CH₂)_nR⁵, —CH(CH₂OH)—CH(OH)-phenyl, —CH(CH₂OH)—C₂H₄—R⁵, —(CH₂)_n—C≡C—C(CH₃)═CH—COR⁵, —CH(Ph)—(CH₂)_n—R⁵, —(CH₂)_n—COR⁵, —(CH₂)_nPO₃(R⁵)₂, —(CH₂)_n—COR⁵, —CH((CH₂)_nOR⁵)CO—R⁵, —(CH₂)_nCONHCH((CH₂)_nR⁵)₂, —(CH₂)_nNH—COR⁵, —CH(CH₂)_nR⁵—(CH₂)_nC₃-C₁₀-cycloalkyl, —(CH₂)_n—C₃-C₁₀-cycloalkyl, C₃-C₁₀-cycloalkyl; C₁-C₆-alkyl that optionally is substituted in one or more places, in the same way or differently, with hydroxy, C₁-C₆-alkyl or the group —COONH(CH₂)_nCH₃ or —NR³R⁴; C₃-C₁₀-cycloalkyl, —(CH₂)_n—O—(CH₂)_n—R⁵, —(CH₂)_n—NR³R⁴, —CH(C₃H₇)—(CH₂)_n—OC(O)—(CH₂)_n—CH₃, —(CH₂)_n—R⁵, —C(CH₃)₂—(CH₂)_n—R⁵—C(CH₂)_n(CH₃)—(CH₂)_nR⁵, —C(CH₂)_n—(CH₂)_nR⁵, —CH(t-butyl)-(CH₂)_n—R⁵, —CCH₃(C₃H₇)—(CH₂)_nR⁵, —CH(C₃H₇)—(CH₂)_n—R⁵, —CH(C₃H₇)—COR⁵, —CH(C₃H₇)—(CH₂)_n—OC(O)—NH—Ph, —CH((CH₂)_n(C₃H₇))—(CH₂)_nR⁵, —CH(C₃H₇)—(CH₂)_n—OC(O)—NH—Ph(OR⁵)₃, —NR³R⁴, —NH—(CH₂)_n—NR³R⁴, R⁵—(CH₂)_n—C*H—CH(R⁵)—(CH₂)_n—R⁵, —(CH₂)_n—CO—NH—(CH₂)_n—CO—R⁵, —OC(O)NH—C₁-C₆-alkyl or —(CH₂)_n—CO—NH—(CH₂)_n—CH—((CH₂)_nR⁵)₂,
  • or for C₃-C₁₀-cycloalkyl, which is substituted with the group
  • or for the group
  • X stands for oxygen or for the group —NH, —N(C₁-C₃-alkyl) or
  • or
  • R² stands for the group
  • or
  • X and R² together form a group
  • A and B, in each case independently of one another, stand for hydrogen, hydroxy, C₁-C₃-alkyl, C₁-C₆-alkoxy or for the group —S—CH₃, —SO₂—C₂H₄—OH, —CO—CH₃, —S—CHF₂, —S—(CH₂)_nCH(OH)CH₂N—R³R⁴, —CH₂PO(OC₂H₅)₂, —S—CF₃, —SO—CH₃, —SO₂CF₃, —SO₂—(CH₂)_n—N—R³R⁴, —SO₂—NR³R⁴, —SO₂R⁷, —CH(OH)—CH₃, —COOH, —CH((CH₂)_nR⁵)₂, —(CH₂)_nR⁵, —COO—C₁-C₆-alkyl, —CONR³R⁴ or for
  • or
  • A and B together can forrn a group
  • R³ and R⁴, in each case independently of one another, stand for hydrogen, phenyl, benzyloxy, C₁-C₁₂-alkyl, C₁-C₆-alkoxy, C₂-C₄-alkenyloxy, C₃-C₆-cycloalkyl, hydroxy, hydroxy-C₁-C₆-alkyl, dihydroxy-C₁-C₆-alkyl, heteroaryl, heterocyclo-C₃-C₁₀-alkyl, heteroaryl-C₁-C₃-alkyl, C₃-C₆-cycloalkyl-C₁-C₃-alkyl that optionally is substituted with cyano, or for C₁-C₆-alkyl that optionally is substituted in one or more places, in the same way or differently, with phenyl, pyridyl, phenyloxy, C₃-C₆-cycloalkyl, C₁-C₆-alkyl or C₁-C₆-alkoxy, whereby the phenyl itself can be substituted in one or more places, in the same way or differently, with halogen, trifluoromethyl, C₁-C₆-alkyl, C₁-C₆-alkoxy or with the group —SO₂NR³R⁴,
  • or for the group —(CH₂)_nNR³R⁴, —CNHNH₂ or —NR³R⁴ or for which optionally can be substituted with C₁-C₆-alkyl,
  • R⁵ stands for hydroxy, phenyl, C₁-C₆-alkyl, C₃-C₆-cycloalkyl, benzoxy, C₁-C₆-alkylthio or C₁-C₆-alkoxy,
  • R⁶ stands for the group
  • R⁷ stands for halogen, hydroxy, phenyl, C₁-C₆-alkyl, —(CH₂)_nOH, —NR³R⁴ or the group
  • R⁸, R⁹ and
  • R¹⁰ stand for hydrogen, hydroxy, C₁-C₆-alkyl or for the group —(CH₂)_n—COOH, and
  • n stands for 0-6, as well as the isomers, diastereomers, enantiomers and salts thereof, have proven quite especially effective.

The compounds according to the invention inhibit CDK II and thus influence the maturation of male and female germ cells.

A method of birth control comprising the inhibition of CDK II is a subject of this invention.

The use of the compounds of general formulas I, II and IIa for the production of a pharmaceutical agent for the selective modulation of germ cell maturation is a subject of this invention.

The eukaryotic cell division cycle ensures the duplication-of the genome and its distribution to the daughter cells by passing through a coordinated and regulated sequence of events. The cell cycle is divided into four subsequent phases: the GI phase represents the time before the DNA replication in which the cell grows and is sensitive to external stimuli. In the S phase, the cell replicates its DNA, and in the G2 phase, preparations are made for entry into mitosis. In mitosis (M phase), the replicated DNA is separated, and the cell division is completed. (FIG. 1) The cyclin-dependent kinases (CDKs), a family of serine/threonine kinases, whose members require the binding of a cyclin (Cyc) as a regulatory subunit in order for them to activate, drive the cell through the cell cycle. For the progression through the S phase and its ending, the activity of the CDK2/CycE and CDK2/CycA complexes is necessary.

Changes in the cell cycle monitoring play a role in meiosis and thus in the maturation of gametes.

The use of the compounds of general formula I for the production of a pharmaceutical agent for contraception is a subject of this invention.

In this invention, it was also possible to show that the described inhibitors of the CDK II have an influence on the sperm concentration if the substances are administered over a period of 21 days (FIG. 2).

It was also possible to show that the weight of the epididymis is reduced when the substance is administered at a concentration of 25 mg/kg daily (FIG. 3). FIG. 4 shows microscopic images of mouse testes. While the testes of control animals (FIG. 4a) do not show any special morphological characteristics, the testes of animals treated with test substance (FIG. 4b) show a physiologically disturbed epithelium. FIG. 4c shows that in the tail of the epididymis of the animals of the test substance group, spermatides were released prematurely from testicular, tubular lumen and were collected in the tail of the epididymis. It was derived therefrom that CDK II is essential for the completion of the 1^st meiosis in male mice.

The use of the compounds of general formulas I, II and IIa for the production of a pharmaceutical agent for the reduction of sperm maturation is a subject of this invention.

In this invention, it was also possible to show that the administration of CDK II inhibitors causes the spontaneous meiotic maturation of mouse egg cells to be inhibited when the test substances are administered at a concentration of 1 μm (FIG. 5).

The use of the compounds of general formulas I, II and IIa for the production of a pharmaceutical agent for inhibiting the egg cell maturation in vitro and in vivo is a subject of this invention.

It was also possible to show that the described substances have an influence on the fertility of female rats. The implantation rate is reduced by substance administration (FIG. 6).

The use of the compounds of general formulas I, II and Ia for the production of a pharmaceutical agent [for] influencing the implantation rate is also a subject of this invention.

Pharmaceutical agents for contraception that contain at least one compound according to general formulas I, II and IIa, as well as pharmaceutical agents with suitable formulation substances and vehicles, are also subjects. ofthis invention.

To use the compounds according to the invention as pharmaceutical agents, the latter are brought into the form of a pharmaceutical preparation, which, in addition to the active ingredient for enteral or parenteral administration, contains suitable pharmaceutical, organic or inorganic inert carrier materials, such as, for example, water, gelatin, gum Arabic, lactose, starch, magnesium stearate, talc, vegetable oils, polylalkylene glycols, etc. The pharmaceutical preparations can be present in solid form, for example as tablets, coated tablets, suppositories, or capsules, or in liquid form, for example as solutions, suspensions or emulsions. Moreover, they optionally contain. adjuvants, such as preservatives, stabilizers, wetting agents, or emulsifiers; salts for changing the osmotic pressure, or buffers.

These pharmaceutical preparations are also subjects of this invention.

For parenteral use, in particular injection solutions or suspensions, in particular aqueous solutions of active compounds in polyhydroxyethoxylated castor oil, are suitable.

As vehicle systems, surface-active adjuvants such as salts of bile acids or animal or plant phospholipids, but also mixtures thereof as well as liposomes or their components can also be used.

For oral use, in particular tablets, coated tablets or capsules with talc and/or hydrocarbon vehicles or binders, such as, for example, lactose, corn or potato starch, are suitable. The use can also be carried out in liquid form, such as, for example, as a juice, to which optionally a sweetener is added.

The dosage of the active ingredients can vary depending on the method of administration, age and weight of the patient, type and severity of the disease to be treated and similar factors. The daily dose is 0.5-1000 mg, preferably 50-200 mg, whereby the dose can be given as a single dose to be administered once or divided into two or more daily doses.

If the production of the starting compounds is not described, the latter are known or can be produced analogously to known compounds or to processes that are described here. It is also possible to perform all reactions that are described here in parallel reactors or by means of combinatory operating techniques.

The isomer mixtures can be separated into enantiomers or E/Z isomers according to commonly used methods, such as, for example, crystallization, chromatography or salt formation.

The production of salts is carried out in the usual way by a solution of the compounds of formulas I, II and IIa being mixed with the equivalent amount or an excess of a base or acid, which optionally is in solution, and the precipitate being separated or the solution being worked up in the usual way.

Production of the Compounds According to the Invention

The examples below explain the production of the compounds according to the invention without limiting the scope of the claimed compounds to these examples.

The compounds of general formula I according to the invention can be produced according to the general procedural schemes below:

[Key to Diagram 3:]

geleche Metliode=Same Method

EXAMPLE 1

Production of 5-Bromo-N2-(4-difluoromethylthiophenyl)-N4-2-propynyl-2,4-pyrimidinediamine (carried out according to process diagram 1) (compound 23)

245 mg (1 mmol) of 2-chloro-4-2-propynylaminopyrimidine is dissolved in 2 ml of acetonitrile, and a suspension of 4-(difluoromethylthio)-aniline hydrochloride [produced from 352 mg (2 mmol) of 4-(difluoromethylthio)-aniline, 1 ml of acetonitrile and 0.5 ml of aqueous HCl (4 M in dioxane)] is added at room temperature. Then, the reaction mixture is refluxed overnight under an N₂ atmosphere. After cooling, the mixture is filtered, the remaining solid phase is washed with H₂O and dried. A yield of 328 mg (85%) of the product can be expected; the melting point is >235° C.

EXAMPLE 2

Production of 5-Bromo-N-(3-(oxiranylmethoxy)phenyl)-2-(2-propynyloxy)-2-pyrimidinamine (compound 51), carried out according to process diagram 2

1.55 g (4.9 mmol) of compound 20 is dissolved in 5.5 ml of epibromohydrin, and 1.38 g of K₂CO₃ and 65 mg of tetrabutylammonium bromide are added thereto. The reaction mixture is stirred for 1 hour at 100° C. under nitrogen atmosphere. After ethyl acetate is added, the resulting precipitate is collected and recrystallized from ethanol. The product yield is 1.15 g (62%) as a white powder; the melting point is approximately 173° C.

Substance 40 is produced analogously to Example 2:

Chromatography: Yield: Melting Point: H/EA 1:3 0.5% TEA 38% 140-141° C.

EXAMPLE 3

Production of 1-(4-((5-Bromo-4-(2-propynyloxy)-pyrimidin-2-yl)-amino)phenoxy)-3-(4-phenylpiperazin-1-yl)-2-propanol (Compound 41)

0.2 ml of a 0.5 M 4-phenylpipetazine solution in DMPU is added to a solution of 19 mg (0.05 mmol) of substance 51 in N,N′-dimethylpropylurea (DMPU). The reaction mixture is kept at a temperature of 80° C. for 18 hours. After cooling, 3.5 ml of tert-butyl methyl ether is added, and the organic phase is extracted 5 times with 1.5 ml of H₂O and then evaporated in a vacuum. The remaining residue is chromatographed on 1.7 g (15 pmol) of Lichrosphere Si60 (gradient: dichloromethane/hexane 1:1 to DCM and then dichloromethane/methanol 99:1 to 93:7). A product yield of 17 mg (64%) is achieved.

Similarly produced are also the following compounds:

28
30
32
33
41
57
58
59
62
65
66
68
72
73
75
76
79
83
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119

The following compounds were produced analogously to the described synthesis processes according to Diagram 1 or 2:

Ex.No.	37	38	39	5
Chromato-	EA + 0.5%	—	Crystallized	—
graphy	TEA		MeOH
Yield	10%	36%	73%	20%
Melting	231° C.	>235° C.	237° C.	157° C.
Point
Ex.No.	16	24	26	35
Chromato-	—	—	—	—
graphy
Yield	94%	86%	73%	69%
Melting	232-234° C.	160° C.	194° C.	143° C.
Point
Ex.No.	27	36	34	21
Chromato-	—	Crystallized	—	—
graphy		EtOH
Yield	69%	64%	87%	59%
Melting	144° C.	219° C.	220° C.	192.5-193.5° C.
Point
Ex.No.	31	25	23	11
Chromato-	BA	DCM/MeOH	—	Crystallized
graphy	Crystallized	95:5		DIPE/EtOH
	H/DIPE
Yield	23%	25%	85%	17%
Melting	198° C.	217-218° C.	>235° C.	>235° C.
Point
Ex.No.	44	45	4
Chromato-	Crystallized	Crystallized	—
graphy	EtOH	EtOH
Yield	27%	48%	52%
Melting	252° C.	235° C.	242-243° C.
Point
Ex.-No.	10	15	3	19
Chromato-	—	—	—	Crystallized
graphy				Water
Yield	43%	27%	76%	52%
Melting	252-253° C.	192-193° C.	257-258° C.	209-210° C.
Point
Ex.No.	9	14	55	50
Chromato-	Crystallized		Crystallized	—
graphy	MeOH		MeOH/DIPE
Yield	24%	91%	27%	56%
Melting	247-248° C.	233-234° C.	228-229° C.	241° C.
Point
Ex.-No.	46	13	52	53
Chromato-	H/EA 1:2	—	—	H/EA 1:2
graphy
Yield	20%	28%	53%	9%
Melting	256° C.	185-186° C.	183° C.	170° C.
Point
Ex.No.	1	54	12	60
Chromato-	Crystallized	Crystallized	Crystallized.
graphy	BA	DIPE/MeOH	BA
Yield	64%	52%	36%
Melting	165.5-166° C.	210° C.	91° C.	150-151° C.
Point
Ex.No.	7	17	2	18
Chromato-		—	—	Crystallized
graphy				MeOH
Yield	25%	10%	62%	50%
Melting	247° C.	201-202° C.	227.5-228.5° C.	245° C.
Point	Decomposi-			Decomposition
	tion
Ex. No.	8(D2O)	86	77
Chromato-	Crystallized	—	—
graphy	Water
Yield	25%	2%	74%
Melting	>275° C.	85° C.	132° C.
Point
Ex.No.	47	6	22	84
Chromato-	MeOH/DCM		—	—
graphy	1:9
Yield	4%	66%	8%	11%
Melting	186-187° C.	146° C.	165-166° C.	152° C.
Point
Ex.No.	40	20
Chromato-	H/EA 1:3	Crystallized
graphy	0.5% TEA	DuPE
Yield	38%	35%
Melting	140-141° C.	174° C.
Point
Ex.No.	49	48	29	42
Chromato-	—	Crystallized	DCM/EA 2:1	HIEA 1:2
graphy		MeOH/DIPE
Yield	9%	16%	26%	29%
Melting	262° C.	150-151° C.		163° C.
Point
Ex.No.	43	55	89	88
Chromato-	H/EA 1:2	Crystallized	—	—
graphy		MeOH/DIPE
Yield	35%	27%	74%	27%
Melting	168° C.	228° C.	248° C.	159° C.
Point			Decomposition	Decomposition
Ex.No.	87	92	91	96
Chromato-	—	H to H/EA 1:1	H to H/EA 1:1	—
graphy
Yield	16%	21%	7%	33%
Melting	210° C.	167-168° C.	105° C.	202° C.
Point
Ex.No.	97	98	90	85
Chromato-	—	—		—
graphy
Yield	23%	32%		53%
Melting	152° C.	172		184° C.
Point
Ex.No.	63	94	93	80
Yield	61%	24%	70%	51%
Melting	220° C.	168° C.	243° C.
Point
Mass	428 (EI)	462 (ES)	494 (ES)	427 (EI)
Ex.No.	120	121	122	123
Yield	49%	24%	80%	73%
Melting	252° C.
Point
Mass	445 (EI)	516 (EI)	334 (EI)	459 (EI)
Ex.No.	95	124	125	126
Yield	29%	25%	27%	46%
Melting	255° C.		218° C.
Point
Mass	425 (EI)	557 (ES)	471 (EI)	449 (EI)
Ex.No.	127	128	129	130
Yield	18%	94%	61%	58%
Melting	220° C.		259° C.	262° C.
Point
Mass	485 (EI)	531 (ES)	403 (EI)	443 (EI)
Ex.No.	131	132	133	134
Yield	9%	42%	25%	64%
Melting	229° C.	141° C.
Point
Mass	491 (EI)	443 (EI)	444 (FAB)
Ex.No.	135	136	137	138
Yield	34%	53%	59%	57%
Melting
Point
Mass	570 (ES)	460 (ES)	549 (ES)	488 (ES)
Ex.No.	139	140	141	142
Yield	20%	63%	23%	8%
Melting
Point
Mass	502 (ES)	382 (ES)	415 (EI)	443 (EI)
Ex.No.	143	144	145	78
Yield	13%	47%	42%	20%
Melting
Point
Mass	392 (EI)	428 (EI)	441 (EI)	541 (ES)
Ex.No.	146	147	148	149
Yield	86%	33%	79%	42%
Melting	225° C.	211° C.	232° C.	241° C.
Point
Mass	408 (EI)	428 (EI)	501 (EI)	411 (ES)
Ex.No.	150	151	152	153
Yield	27%	65%	85%	9%
Melting				231° C.
Point
Mass	420 (ES)	395 (ES)	468 (ES)	395 (ES)
Ex.No.	154	155	156	157
Yield	90%	48%	77%	21%
Melting	170° C.	181° C.	177° C.	196° C.
Point
Mass	381 (ES)	409 (ES)	394 (EI)	391 (EI)
Ex. No.	158	159 *	160 *	161 *
Yield	37%	21%	14%	8%
Melting			199° C.	>300° C.
Point
Mass	469 (EI)	468 (EI)468 (EI)	508 (EI)
Ex.No.	162	163*	164	165
Yield	16%	33%	14%	51%
Melting	195° C.			162-164° C.
Point
Mass	446 (ES)	480 (EI)	429 (ES)	462 (EI)
Ex.No.	166	167 *	168 *	169
Yield	6%	16%	58%	60%
Melting		256° C.	261° C.
Point
Mass	390 (ES)	512 (ES)	538 (ES)	484 (ES)
Ex.No.	170 *	171	172	173
Yield	64%	7%	65%	40%
Melting	226° C.	164° C.	206° C.	144° C.
Point
Mass	525 (ES)	488 (ES)	395 (ES)	397 (ES)
Ex.No.	174 *	175 *	176	177
Yield	95%	51%	3%	8%
Melting
Point
Mass	511 (ES)	511 (ES)	443 (EI)	456 (EI)
Ex.No.	178	179	180	181
Yield	17%	9%	27%	24%
Melting
Point
Mass	427 (EI)	428 (ED	472 (ES)	486 (ES)
Ex.No.	182	183	184	185
Yield	57%	78%	26%	76%
Melting
Point
Mass	639 (ES)	439 (EI)	348 (EI)	445 (EI)
Ex.No.	186	187	188	189
Yield	16%	7%	61%	35%
Melting
Point
Mass	440 (ES)	480 (ES)	443 (EI)	321 (EI)
Ex.No.	190	191 *	192 *	193
Yield	63%	15%	17%	57%
Melting
Point
Mass	437 (EI)	511 (ES)	511 (EI)	403 (EI)
Ex.No.	194	195	196	197
Yield	26%	56%	12%	61%
Melting
Point
Mass	476 (EI)	417 (EI)	450 (EI)	479 (EI)
Ex.No.	198	199	200	201
Yield	4%	4%	7%	2%
Melting
Point
Mass	439 (EI)	439 (EI)	515 (ES)	515 (ES)
Ex. No.	202	203 *	204 *	205
Yield	10%	2%	2%	16%
Melting
Point
Mass	483 (ES)	480 (EI)	480 (EI)	430 (ES)
Ex.No.	206	207	208	209
Yield	5%	55%	44%	77%
Melting	223° C.	248° C.	228° C.	231° C.
Point
Mass	446 (ES)	507 (EI)	514 (EI)
Ex.No.	210	211	212	71
Yield	86%	22%	41%	77%
Melting
Point
Mass	528 (CI)	429 (EI)	352 (EI)	437 (EI)
Ex.No.	213	61	214	215
Yield	49%	25%	2%	9%
Melting
Point
Mass	365 (EI)	379 (EI)	443 (ES)	444 (ES)
Ex.No.	216	217	218	219
Yield	65%	34%	58%	88%
Melting	239° C.	239° C.	238° C.	280° C.
Point
Mass	439(EI)	413(EI)	439(EI)	416(EI)
Ex.No.	74	56	220	221
Yield	7%	17%	65%	19%
Melting	285° C.	158° C.	166° C.
Point
Mass	457 (EI)	392 (EI)	354 (EI)	522 (ES)
Ex.No.	222	223	224	225
Yield	54%	23%	7%	43
Melting	300° C.	300° C.		243° C.
Point
Mass	501 (EI)	465 (EI)		434 (EI)
Ex.No.	226	227	228	229
Yield	47%	41%	88%	89%
Melting	229° C.	287° C.	259° C.	233° C.
Point
Mass	440 (CI)	434 (EI)	451 (EI)	463 (EI)
Ex. No.	230
Yield	58%
Melting	>300° C.
Point
Mass	466 (ES)
Ex.No.	231	232	233	234
Yield	85%	35%	33%	25%
Melting
Point
Mass	330 (EI)	288 (EI)	389 (CI)	448 (ESI)
Ex.No.	235	236	237	238
Melting
Point
Mass	486 (ES)	516 (ES)	504 (ES)	488 (ES)
Ex.No.	239	240	241	242
Melting
Point
Mass	536 (ES)	502 (ES)	484 (ES)	551 (ES)
Ex.No.	243	244	245
Melting
Point
Mass	516 (ES)	514 (ES)	433 (ES)
Ex.No.	246	247.	248	249
Melting			205° C.	>300° C.
Point
Mass	446 (ES)	415 (EI)	504 (ES)	431 (ES)
Ex.No.	250	251	252	253
Melting	113° C.	231° C.	187° C.
Point
Mass	488 (ES)	446 (ES)	433 (ES)
Ex.No.	254	255	256	257
Melting
Point
Mass	399 (ES)	444 (ES)	474 (ES)	486 (ES)
Compounds Nos. 159, 160, 161, 163, 167, 168, 170, 174, 175, 191, 192, 203 and 204 that are identified by * ) can be produced by the process variant described under Example No. 295.

EXAMPLE 258

Production of 4-(5-Bromo-4-morpholin-4-yl-pyrimidin-2-ylamino)-phenylsulfonamide

202 mg (0.60 mmol) of the compound of Example No. 122 is mixed with 1 ml of water as well as 0.2 g (1.2 mmol) of bromine and stirred at room temperature. After 24-hours, 0.2 g (1.2 mmol) of bromine is added again and stirred for another 24 hours at room temperature. The solvent is evaporated by means of underpressure, and the remaining residue is purified by chromatography (Flashmaster II, DCM/MeOH 7:3). The yield is 17 ing (0.04 mmol, 7%) of the product as a white solid.

Ex.No.	259	260	261	262
Melting		205-207° C.	202-203° C.
Point
Mass	MS (ES) 452, 454			428 (ES)
	(M + H, 100%)
Ex. No.	263	264	265
ESI-MS	434	434	477
Ex. No.	266	267	268
ESI-MS	477	552	552

Analogously to production example 1, the following compounds were also produced:

Ex.No.	272	273	274	275
Yield	61%	44%	42%	68%
Mass	EI:	EI:	ESI:	EI:
	M+ 463 (4%)	M+ 403 (24%)	MH+ 418 100%	M+ 401 (33%)
	277 (8%)	358 (100%)	416 (94%)	372 (100%)
	105 (100%)	277 (52%)	346 (8%)	344 (38%)
Ex. No.	276	277	278	279
Yield	81%	58%	20%	30%
Mass	EI:	ESI:	ESI:	ESI:
	M+ 431	MH+ 444	MH+ 494 (75%)	MH+ 418
	(5%)	(100%)	346 (18%)	(100%)
	372 (100%) 442 (97%)	214 (55%)	416 (97%)
	291 (46%)	115 (20%)		310 (27%)
Ex.No.	280	281	282	283
Yield	55%	43%	˜18%	35%
Mass	ESI:	ESI:	ESI:	ESI:
	MH+ 444	MH+ 446	MH+ 416	MH+ 446
	(100%)	(100%)	(100%)	(100%)
	442 (97%)	444 (95%)	414 (96%)	444 (90%)
	214 (12%)	346 (5%)	317 (4%)
Ex.No.	284	285	286	287
Yield	51%	46%	47%	61%
Mass	ESI:	ESI:	ESI.	ESI.
	MH+ 520	MH+ 520	MH+ 432	MIH+ 446
	(100%)	(100%)	(100%)	(100%)
	518 (97%)	518 (97%)	430 (95%)	444 (93%)
	115 (27%)	115 (23%)	346 (5%)	115 (13%)

According to subsequent production variants, the following compounds are also synthesized:

30 mg (0.0678 mmol) of compound No. 277 is dissolved in 1 ml of methanol/tetrahydrofuran 1:1. After 10 mg of sodium borohydride is added, it is stirred for 2 more hours. Then, it is quenched with 3-4 drops of glacial acetic acid while being cooled and concentrated by evaporation. The crude product is subsequently taken up with a little water, suctioned off, rewashed with acetonitrile and dried at 60° C. in a vacuum. Yield: 21 mg (70% of theory) of the desired compound.

Ex. No.	288	289
Yield	52%	70%
Mass	EI:	ESI:
	M+ 465 (5%)	MH+ 446 (100%)
	358 (40%)	444 (93%)
	207 (31%)	117 (20%)

EXAMPLE 290

Production of the Oxime Ether-Pyrimidine Compounds of General Formula I

The production of oxime ether is carried out according to the following general reaction diagram:

• • R⁸ and R⁹ have the meanings that are indicated in general formula I.

PRODUCTION OF EXAMPLE 290

50 mg (0.12 mmol) of compound No. 282, 34 mg of hydroxylammonium chloride and 150 mg of pulverized KOH are refluxed for 2 hours in 2 ml of ethanol. Then, it is poured into ice water and acidified with glacial acetic acid, extracted 3 times with dichloromethane/isopropanol 4:1, dried and concentrated by evaporation with magnesium sulfate. The residue is suspended with acetonitrile, suctioned off and dried at 60° C. Yield: 28 mg (54% of theory) of the desired compound.

Mass: ESI:

MH⁺ 429 (29%)

371 (61%)

289 (91%)

The following compounds were also produced analogously:

Ex.No.	291	292	293
Yield	34%	36%	40%
Mass	ESI:	ESI:	ESI:
	MH+ 443 (95%)	MH+ 485 (92%)	MH+ 487 (91%)
	445 (99%)	487 (99%)	489 (89%)
	373 (32%)		373 (32%)

EXAMPLE 294

Reductive Amination

50 mg (0.12 mmol) of compound No. 282 and 7.5 mg (0.132 mmol) of cyclopropylamine are dissolved in 2 ml of 1,2-dichloroethane. After 9.1 mg (0.144 mmol) of sodium cyanoborohydride is added, it is allowed to stir for 12 more hours. Then, it is diluted with dichloromethane/isopropanol 4:1, washed twice with water, dried with magnesium sulfate and then concentrated by evaporation. The residue is chromatographed on silica gel with dichloromethane/methanol 95:5. Yield: 18 mg (33% of theory) of the desired compound.

Yield 33%
Mass  ESI:
      MH+ 457
      (98%)
      455 (93%)
      249 (55%)

Similarly produced are also compounds Nos. 159, 160, 161, 163, 167, 168, 170, 174, 175, 191, 192, 203 and 204.

EXAMPLES 295 AND 296

Produced in a way similar to Example 1 are also the following two compounds:

Example	295	296
Yield	46%	47%
Mass	ESI:	ESI:
	MH+ 432 (30%)	MH+ 446 (45%)
	434 (31%)	448 (49%)
	123 (100%)	123 (90%)

Production of the Sulfonamides of General Formula I

0.2 mmol of sulfonic acid fluoride is introduced into a reactor of a synthesizer, and 1.0 ml of solvent, preferably 2-butanol, is added. 0.2 ml (0.2 mmol) of DMAP—dissolved in a solvent, for example DMSO or 2-butanol—and 0.2 ml (0.2 mmol) of amine, dissolved in 2-butanol, are added in succession via a pipette. The reaction mixture is then stirred for 20 hours at 80° C. After the reaction is completed, the crude product is pipetted off, and the reactor is rewashed with 1.0 ml of THF. The solution of the crude product is then concentrated by evaporation and purified by means of HPLC.

The following compounds were produced:

Ex.No.	297	298	299	300
Molecular	526.4968	562.5298	624.6006	501.4471
Weight
ESI-MS	526/528	562/564	624/626	501/503
Ex. No.	301	302	303	304
Molecular	538.4682	588.4465	528.5126	542.5394
Weight
ESI-MS	538/540	588/590	528/530	542/544
Ex.No.	305	306	307	308
Molecular	556.5662	570.593	510.4106	588.4465
Weight
ESI-MS	556/558	570/572	510/512	588/590
Ex.No.	309	310	311	312
Molecular	548.503	555.4949	500.459	514.4858
Weight
ESI-MS	548/550	555/557	500/502	514/516
Ex.No.	313	314	315	316
Molecular	515.4739	557.5543	470.3896	551.5069
Weight
ESI-MS	515/517	557/559	470/472	55 1/553
Ex.No.	317	318	319	320
Molecular	534.4762	568.9213	524.4374	543.4839
Weight
ESI-MS	534/536	568/570	524/526	543/545
Ex. No.	321	322	323	324
Molecular	488.4044	526.4776	564.502	527.4849
Weight
ESI-MS	488/490	526/528	564/566	527/529
Ex.No.	325	326	327	328
Molecular	541.5117	538.4395	541.5117	521.4375
Weight
ESI-MS	541/543	538/540	541/543	521/523
Ex.No.	329	330	331	332
Molecular	538.4395	521.4375	550.4752	550.4752
Weight
ESI-MS	538/540	521/523	550/552	550/552
Ex. No.	333	334	335	336
Molecular	613.5551	534.4762	512.47	548.503
Weight
ESI-MS	613/615	534/536	512/514	548/550
Ex. No.	337	338	339	340
Molecular	610.5738	487.4203	524.4414	574.4197
Weight
ESI-MS	610/612	487/489	524/526	574/576
Ex.No.	341	342	343	344
Molecular	514.4858	528.5126	542.5394	556.5662
Weight
ESI-MS	516/514	528/530	542/544	556/558
Ex.No.	345	346	347	348
Molecular	496.3838	574.4197	534.4762	541.4681
Weight
ESI-MS	496/498	574/576	534/536	541/543
Ex.No.	349	350	351	352
Molecular	486.4322	500.459	501.4471	543.5275
Weight
ESI-MS	486/488	500/502	501/503	543/545
Ex.No.	353	354	355	356
Molecular	456.3628	537.4801	520.4494	554.8945
Weight
ESI-MS	456/458	537/539	520/522	554/556
Ex.No.	357	358	359	360
Molecular	510.4106	529.4571	474.3776	512.4508
Weight
ESI-MS	510/512	529/531	474/476	541/514
Ex.No.	361	362	363	364
Molecular	550.4752	513.4581	527.4849	524.4127
Weight
ESI-MS	550/552	513/515	527/529	524/526
Ex.No.	365	366	367	368
Molecular	527.4849	507.4107	524.4127	507.4107
Weight
ESI-MS	527/529	507/509	524/526	507/509
Ex.No.	369	370	371	372
Molecular	536.4484	536.4484	599.5283	520.4494
Weight
ESI-MS	536/538	536/538	599/601	520/522
Ex.No.	373	374	375	376
Molecular	512.47	548.503	610.5738	524.4414
Weight
ESI-MS	512/514	548/550	610/612	524/526
Ex.No.	377	378	379	380
Molecular	574.4197	514.4858	528.5126	542.5394
Weight
ESI-MS	574/576	514/516	528/530	542/544
Ex.No.	381	382	383	384
Molecular	496.3838	574.4197	534.4762	541.4681
Weight
ESI-MS	496/498	574/576	534/536	541/543
Ex.No.	385	386	387	388
Molecular	486.4322	500.459	501.4471	543.5275
Weight
ESI-MS	486/488	500/502	501/503	543/545
Ex.No.	389	390	391	392
Molecular	537.4801	520.4494	554.8945	510.4106
Weight
ESI-MS	537/539	520/522	554/556	510/512
Ex.No.	393	394	395	396
Molecular	529.4571	474.3776	512.4508	513.4581
Weight
ESI-MS	529/531	474/476	512/514	513/515
Ex.No.	397	398	399	400
Molecular	527.4849	524.4127	527.4849	507.4107
Weight
ESI-MS	527/529	524/526	527/529	507/509
Ex.No.	401	402	403	404
Molecular	524.4127	507.4107	536.4484	536.4484
Weight
ESI-MS	524/526	507/509	526/538	536/538
Ex.No.	405	406	407	408
Molecular	599.5283	520.4494	529.4419	534.4762
Weight
ESI-MS	599/601	520/522	529/531	534/536
Ex.No.	409	410	411	412
Molecular	596.547	473.3935	510.4146	560.3929
Weight
ESI-MS	596/598	473/475	510/512	560/562
Ex.No.	413	414	415	416
Molecular	500.459	514.4858	528.5126	482.357
Weight
ESI-MS	500/502	514/516	528/530	482/484
Ex.No.	417	418	419	420
Molecular	560.3929	520.4494	527.4413	472.4054
Weight
ESI-MS	560/562	520/522	527/529	472/474
Ex.No.	421	422	423	424
Molecular	486.4322	487.4203	529.5007	523.4532
Weight
ESI-MS	486/488	487/489	529/531	523/525
Ex.No.	425	426	427	428
Molecular	506.4226	540.8677	496.3838	515.4303
Weight
ESI-MS	506/508	540/542	496/498	515/517
Ex.No.	429	430	431	432
Molecular	460.3508	498.424	499.4313	513.4581
Weight
ESI-MS	460/462	498/500	499/501	513/515
Ex.No.	433	434	435	436
Molecular	510.3859	513.4581	493.3839	510.3859
Weight
ESI-MS	510/512	513/515	493/495	510/512
Ex.No.	437	438	439	440
Molecular	493.3839	522.4216	522.4216	585.5015
Weight
ESI-MS	493/495	522/524	522/524	585/587
Ex.No.	441	442	443 *
Molecular	506.4226	515.4151	416.30
Weight
ESI-MS	506/508	515/517	416/418
* Produced according to the process that is described under “Sulfonamides”

Production of the Pyrimidine-Sulfonyl Fluorides of General Formula I

The production of pyrimidine-sulfonic acid fluorides is carried out analogously to the production of sulfonic acid amides.

Ex. No.	444	445	446	447
Molecular	405.25	419.27	419.27	433.30
Weight
ESI-MS	217-220	196-202	165-196	198-204
	405/407	419/421	419/421	433/435
Ex. No.	448	449	450	451
Molecular	433.30	447.33	405.25	419.27
Weight
ESI-MS	144-149	219-222	170-173	226-228
	433/435	447/449	405/407	419/421
Ex. No.	452	453	454	455
Molecular	433.30	447.33	433.30	419.27
Weight
ESI-MS	433/435	447/449	433/435	419/421

Produced similarly to the above-described examples were also the following para-compounds:

Ex. No.	456	457	458	459
Molec-	498.4432	534.4762	596.547	473.3935
ular
Weight
ESI-MS	498/500	534/536	596/598	473/475
Ex. No.	460	461	462	463
Molec-	510.4146	560.3929	500.459	514.4858
ular
Weight
ESI-MS	510/512	560/562	500/502	514/516
Ex. No.	464	465	466	467
Molec-	528.5126	542.5394	560.3929	520.4494
ular
Weight
ESI-MS	528/530	542/544	560/562	520/522
Ex. No.	468	469	470	471
Molec-	527.4413	472.4054	486.4322	529.5007
ular
Weight
ESI-MS	527/529	472/474	486/488	529/531
Ex. No.	472	473	474	475
Molec-	442.336	523.4532	506.4226	540.8677
ular
Weight
ESI-MS	442/444	523/525	506/508	540/542
Ex. No.	476	477	478	479
Molec-	496.3838	515.4303	460.3508	498.424
ular
Weight
ESI-MS	496/498	515/517	460/462	498/500
Ex. No.	480	481	482	483
Molec-	536.4484	499.4313	513.4581	510.3859
ular
Weight
ESI-MS	536/538	499/501	513/515	510/512
Ex. No.	484	485	486	487
Molec-	513.4581	493.3839	510.3859	493.3839
ular
Weight
ESI-MS	513/515	493/495	510/512	493/495
Ex. No.	488	489	490	491
Molec-	522.4216	522.4216	585.5015	506.4226
ular
Weight
ESI-MS	522/524	522/524	585/587	506/508
Ex. No.	492	493	494
Molec-	515.4151	512.47	548.503
ular
Weight
ESI-MS	515/517
Ex. No.	495	496
Molec-	610.5738	487.4203
ular
Weight
ESI-MS
Ex. No.	497	498
Molec-	524.4414	574.4197
ular
Weight
ESI-MS
Ex. No.	499	500
Molec-	514.4858	528.5126
ular
Weight
ESI-MS
Ex. No.	501	502
Molec-	542.5394	556.5662
ular
Weight
ESI-MS
Ex. No.	503	504
Molec-	496.3838	574.4197
ular
Weight
ESI-MS
Ex. No.	505	506
Molec-	534.4762	541.4681
ular
Weight
ESI-MS
Ex. No.	507	508
Molec-	486.4322	500.459
ular
Weight
ESI-MS
Ex. No.	509	510
Molec-	501.4471	543.5275
ular
Weight
ESI-MS
Ex. No.	511	512
Molec-	456.3628	537.4801
ular
Weight
ESI-MS
Ex. No.	513	514
Molec-	520.4494	566.4742
ular
Weight
ESI-MS
Ex. No.	515	516
Molec-	554.8945	510.4106
ular
Weight
ESI-MS
Ex. No.	517	518
Molec-	529.4571	474.3776
ular
Weight
ESI-MS
Ex. No.	519	520
Molec-	512.4508	550.4752
ular
Weight
ESI-MS
Ex. No.	521	522
Molec-	513.4581	527.4849
ular
Weight
ESI-MS
Ex. No.	523	524
Molec-	524.4127	527.4849
ular
Weight
ESI-MS
Ex. No.	525	526
Molec-	507.4107	524.4127
ular
Weight
ESI-MS
Ex. No.	527	528
Molec-	507.4107	536.4484
ular
Weight
ESI-MS
Ex. No.	529	530
Molec-	536.4484	599.5283
ular
Weight
ESI-MS
Ex. No.	531	532
Molec-	520.4494	529.4419
ular
Weight
ESI-MS

Separation of Diastereomer Mixtures of the Compounds According to the Invention

Separation in the Example of the Diastereomer Mixture of Compound No. 274

The diastereomer mixture was seperated into the two corresponding racemates (A and B) by means of HPLC.

Conditions

Column:          Kromasil C18 (5 μm) 150 × 4.6 mm
Eluant:          25% acetonitrile/water with 1 ml of NH3/1;
Flow:            1.0 ml/min
Detection:       PDA 300 nm
Retention Times: Racemate A - 11.6 minutes
                 Racemate B - 12.4 minutes

NMR	DMSO-d6:	DMSO-d6:
	9.68, s, 1H	9.68, s, 1H
	8.12, s, 1H	8.11, s, 1H
	7.87, d, 2H	7.85, d, 2H
	7.70, d, 2H	7.69, d, 2H
	7.14, s, 2H	7.16, s, 2H
	6.15, d, 1H	6.35, d, 1H
	5.01, d, 1H	4.90, d, 1H
	4.10, m, 1H	4.08, m, 1H
	3.80, m, 1H	3.80, m, 1H
	1.22, d, 3H	1.18, d, 3H
	1.1, d, 3H	1.12, d, 3H

Below, racemates A and B in each case were separated by means of chiral HPLC.

Conditions:

Column:          Chiralpak AD (10 μm) 250 × 4.6 mm
Eluant:          Hexane/Ethanol 80:20
Flow:            1.0 ml/min
Detection:       PDA 300 nm
Retention Times: Enantiomer A1 - 16.6 min
                 Enantiomer A2 - 19.6 min
                 Enantiomer B1 - 16.0 min
                 Enantiomer B2 - 17.8 min

The production of the intermediate stages that are preferably used for the synthesis of the compounds of general formula I according to the invention is described in WO 02/096888.

Another subject of this invention is also the use of compounds for birth control that fall under the industrial-property right DE 4029650 and whose action is in the fungicide range and that are described in the WO as CDK inhibitors. Use of this compound for birth control is not described to date.

No.	Structure	No.	Structure	No.	Structure
5		6		22		23
16		24		35		37
38		42		43		50
54		70		81		82

In addition, the invention thus relates to the use of pharmaceutical agents for birth control, comprising a compound of general formula I

• • in which
  • R¹ stands for halogen or C₁-C₃-alkyl,
  • X stands for oxygen or —NH,
  • A stands for hydrogen,
  • B stands for hydroxy, —CO-alkyl-R⁷, —S—CHF₂, —S—(CH₂)_nCH(OH)CH₂N—R³R⁴, —S—CF₃, or —CH—(OH)—CH₃, or
  • A and B, independently of one another, can form a group

R², R³, R⁴, R⁷ and R⁸ have the meanings that are indicated in general formula I, as well as the isomers, diastereomers, enantiomers and salts.

The compounds according to the invention can be used for the production of a pharmaceutical agent for non-hormonal contraception.

In addition, a subject of this invention is the use of those compounds for birth control that fall under the industrial-property right U.S. Pat. No. 6,515,004 and also under the scope of protection of the application WO 01/44242 and whose action as inhibitors of the CDK-dependent kinases, whose use as agents for the production of a pharmaceutical agent for contraception is not disclosed to date, however, is known.

EXAMPLE STRUCTURE

Example
Number Structure
533

In addition, the invention thus relates to the use comprising a compound of general formula II

• • in which
  • R¹ stands for a C₁-C₁₀-alkyl group,
  • R² stands for hydrogen or alkyl,
  • X stands for NR₂ or CHNR₂R₃,
  • R¹ and R², independently of one another, stand for hydrogen, alkyl, substituted alkyl, cycloalkyl or substituted cycloalkyl, and
  • n=0, 1, 2, 3, as well as the isomers, enantiomers, diastereomers and salts thereof for the production of a pharmaceutical agent for non-horrnonal contraception.

The compounds have proven to be especially effective in which

• • R¹ stands for a C₁-C₁₀-alkyl group,
  • R² stands for hydrogen,
  • X stands for NR2 or CHNR₂R₃,
  • R¹ and R², independently of one another, stand for hydrogen, alkyl, substituted alkyl, cycloalkyl or substituted cycloalkyl, and
  • n=2, as well as the isomers, enantiomers, diastereomers and salts thereof.

The compound of formula Ia as well as the isomers, enantiomers, diastereomers and salts thereof and in which R² stands for hydrogen, alkyl, substituted alkyl or cycloalkyl, have proven to be quite especially effective.

BIOLOGICAL EXAMPLES

1. Influence of CDK II Inhibitors on Spermatogenesis in Mice.

The tests are performed on adult male mice (mouse strain C57BL/6) with a weight of 25-30 g.

The animals are kept in Makrolon cages in spaces with controlled light (12 hours of darkness, 12 hours of light with a 30-minute twilight) and fed a standard diet (palletized SNIFF rat-mouse holder, 10 mm diameter) and supplied with as much tap water as they want.

1.1 Formulation and Administration of Test Substances:

The test substances are dissolved in dimethyl sulfoxide +0.9% common salt solution (1+1 v/v).

Altogether, 12 animals are treated with each test substance. In this case, they are divided into 3 groups that in each case obtained different amounts of test substances.

Group 1	Vehicle (0.9% common salt solution 1 + 1 v/v))	4 Animals
Group 2	10 mg/kg/d of test substance	4 Animals
Group 3	25 mg/kg/d of test substance	4 Animals

The test substance is administered subcutaneously over a period of 21 days via a mini-osmotic pump (Alza Company, Palo Alto, USA), Model 2002 (pump volume 0.5 μl/hour). The pumps must be changed after 14 days and replaced with new ones. They ensure a continuous administration of test substance.

1.2 Test Preparation:

The mini-osmotic pumps are stored for one day before the implantation with the test substances overnight in 0.9% common salt solution at 37° C. This step is important in order to ensure a uniform delivery.

The animals are anesthetized with diethyl ether. The fur on the back is disinfected with 70% ethanol and opened up with shears. With pointed forceps, a pocket is formed between the fur and back muscles in which the mini-osmotic pump is implanted. The wound is closed with Michel's sutures.

On day 21, the animals are anesthetized with diethyl ether, the abdominal cavity is opened up and exsanguinated by puncturing the body cavity veins. The blood serum that is obtained by centrifuging is used to determine LH, FSH and testosterone.

In the autopsy, thymus gland, spleen, testis, epididymis, seminal vesicles, prostate, liver and kidneys are prepared outside, and the weights are determined. The organs are worked up histologically (formalin-fixed and embedded in paraffin). Sections of the testes are stained with hematoxylin/eosin. In selected sections of the testes, in addition an apoptosis staining (protocol according to manufacturer information, Roche, Kit: In-Situ Cell Death Detection Kit AP, Cat. 1684809) is performed.

To determine sperm motility, the distal portion of an epididymis is removed (epididymis tail), cut, and transferred for 5 minutes in 500 μl of Dulbecco's PBS buffer that is 37° C. Then, the tissue portions are removed, and the motility of the sperm found in the buffer is examined by microscope.

To examine the sperm morphology, 20 μl of sperm suspension is dropped onto a slide and dried overnight. On the next day, an HE staining of the sperm smears is carried out.

To determine the sperm concentration, a 1:20 dilution (10 μl of parent suspension+190 μl of distilled water) is produced. To count the sperm in the Neugebauer counting chamber, 10 μl of suspension is used.

2. Inhibition of the Spontaneous Meiotic Maturation of Mouse Egg Cells

Egg cells are found before the so-called LH peak (luteinizing hormone), which injects the ovulation-triggering processes, in a state of meiotic arrest, which can be detected by the visibility of germinal vesicles (GV). If these egg cellsare isolated from the follicles that surround them, a spontaneous meiotic maturation sets in, since now inhibitory factors of the follicles are lacking, which otherwise maintain this meiotic arrest. Optically, the meiotic maturation is identified by the disappearance of the GV, the so-called germinal vesicle breakdown (GVB) and in the festering behavior in the ejection of the first polar body (PB).

In juvenile mice, the egg cell maturation is induced by injection of 10 IU of PMSG (Pregnant Mare Serum Gonadotrophin, i.p.). After 48 hours, the egg cells are isolated from antral follicles. In the medium, in which no specific inhibitors of meiosis are present, the spontaneous meiotic maturation to the GVB and PB stage begins. A measuring parameter is the influence of test substances on the spontaneous meiotic maturation.

3. Measurement of the Fertility of Female Rats:

Female rats have a stable four-day cycle. The cycle stages can be determined based on vaginal smears. If the animals in the estrus stage are bred with a male animal, in most cases a pregnancy results with 10-17 implantation sites.

Therefore, substances can be tested for their influence on fertility by the test substance being administered to female rats at different points in the cycle, being bred during estrus and e.g., the number of implantation sites being counted on day 16 after the breeding.

Test substances are administered once daily p.o., for example in a dose of 50 mg/kg over 4 days, beginning in the diestrus stage, whereby the animals in the estrus stage were bred. In this connection, there was a 35% reduction in the implantation sites, determined in the autopsy on day 16 after the breeding, relative to the vehicle control (8.2±6.1 versus 12.8±1.3; with n=6).

Test substances are administered twice daily, p.o., for example at a dose of 50 mg/kg over 2 days, beginning in the diestrus stage, whereby the animals in the estrus stage were bred. In this connection, there was a 56% reduction in the implantation sites, determined in the autopsy on day 16 after the breeding, relative to the vehicle control (6.0±3.5 versus 13.7±1.5; with n=6).

4. CDK 2/CycE Kinase Assay

Recombinant CDK2 and CycE-GST fusion proteins, purified from baculovirus-infected insect cells (Sf9), were obtained by Dr. Dieter Marmé, Klinik für Tumorbiologie [Clinic for Tumor Biology], Freiburg. Histone IIIS, which was used as a kinase substrate, was purchased from the Sigma Company.

CDK2/CycE (50 ng/measuring point) is incubated for 15 minutes at 22° C. in the presence of different concentrations of test substances (0 μm, as well as within the range of 0.01-100 μm) in assay buffer [50 mmol of tris/HCl, pH 8.0, 10 rnmol of MgCl2, 0.1 rnmol of Na ortho-vanadate, 1.0 mmol of dithiothreitol, 0.5 μm of adenosine trisphosphate (ATP), 10 μg/measuring point of histone IIIS, 0.2 μCi/measuring point of 33P-gamma ATP, 0.05% NP40, 1.25% dimethyl sulfoxide]. The reaction is halted by adding EDTA solution (250 mmol, pH 8.0, 14 μl/measuring point). From each reaction batch, 10 μl is applied to P30 filter strips (Wallac Company), and non-incorporated 33P-ATP was removed by subjecting the filter strips to three washing cycles for 10 minutes each in 0.5% phosphoric acid. After the filter strips are dried for 1 hour at 70° C., the filter strips are covered with scintillator strips (MeltiLex™ A, Wallac Company) and baked for 1 hour at 90° C. The amount of incorporated 33P (substrate phosphorylation) is determined by scintillation measurement in a gamma-radiation measuring device (Wallac).

FIGURES

FIG. 1 shows a graphic visualization of the sperm concentration.

FIG. 2 shows a graphic visualization of the organ weights.

FIG. 3 shows a microscopic image of the mouse testis:

• • a. Histological section through a mouse testis of a vehicle-treated mouse (₂₀th lens)
  • b. 25 mg/kg/d of test substance (20^th lens); stars indicate the testicular tubuli that have a physiologically disturbed epithelium. Postmeiotic sperm stages do not seem to be present.
  • c. Mouse cauda epididymis (tail of the epididymis), 25 mg/kg/d of test substance (10^th lens). The arrows point to the spermatides that were released prematurely by testicular tubular lumen and were collected in the tail of the epididymis.

FIG. 4 shows the influence of a test substance on the spontaneous maturation of the egg cells (mouse). In this connection, the test substance was administered at a concentration of 0.1, 1 and 10 mmol; ethanol was used as a vehicle, n=8-10.

FIG. 5 shows the influence of a test substance on the fertility of female rats, 50 m/kg was administered twice daily. HP-β-CD (pH 5) was used as a vehicle.

Claims

1. Method of birth control comprising the inhibition of CDK II.

2. Use of the compounds of general formula I

3. Use of the compounds according to claim 1in which R1 stands for hydrogen, halogen, C1-C6-alkyl, nitro or for the group —COR5, —OCF3, —(CH2)nR5, —S—CF3 or —SO2CF3, R2 stands for C1-C10-alkyl, C2-C10-alkenyl, C2-C]0-alkinyl or C3-C10-cycloalkyl or for C1-C10-alkyl, C2-C10-alkenyl, C2-C10-alkinyl or C3-C10-cycloalkyl that is substituted in one or more places, in the same way or differently, with hydroxy, halogen, C1-C6-alkoxy, C1-C6-alkylthio, amino, cyano, C1-C6-alkyl, —NH—(CH2)n—C3-C10-cycloalkyl, C3-C10-cycloalkyl, C1-C6-hydroxyalkyl, C2-C6-alkenyl, C2-C6-alkinyl, C1-C6-alkoxy-C1-C6-alkyl, C1-C6-alkoxy-C1-C6-alkoxy-C1-C6-alkyl, —NHC1-C6-alkyl, —N(C1-C6-alkyl)2, —SO(C1-C6-alkyl), —SO2(C1-C6-alkyl), C1-C6-alkanoyl, —CONR3R4, —COR5, C1-C6-alkylOAc, carboxy, aryl, heteroaryl, —(CH2)n-aryl, —(CH2)n-heteroaryl, phenyl-(CH2)n—R5, —(CH2)nPO3(R5)2 or with the group —R6 or —NR3R4, and the phenyl, C3-C10-cycloalkyl, aryl, heteroaryl, —(CH2)n-aryl and —(CH2)n-heteroaryl itself optionally can be substituted in one or more places, in the same way or differently, with halogen, hydroxy, C1-C6-alkyl, C1-C6-alkoxy, heteroaryl, benzoxy or with the group —CF3 or —OCF3, and the ring of C3-C10-cycloalkyl and C1-C10-alkyl optionally can be interrupted by one or more nitrogen, oxygen and/or sulfur atoms, and/or can be interrupted by one or more ═C═O groups in the ring and/or optionally one or more possible double bonds can be contained in the ring, or R2 stands for the group X stands for oxygen or for the group —NH—, —N(C1-C3-alkyl) or for —OC3-C10-cycloalkyl that can be substituted in one or more places, in the same way or differently, with a heteroaromatic compound, or X and R2 together form a C3-C10Icycloalkyl ring, which. optionally can contain one or more heteroatoms and optionally can be substituted in one or more places with hydroxy, C1-C6-alkyl, C1-C6-alkoxy or halogen, A and B, in each case independently of one another, stand for hydrogen, hydroxy, C1-C3-alkyl, C1-C6-alkoxy or for the group —S—CH3, —SO2—C2H4—OH, —CO—CH3, —S—CHF2, —S—(CH2)n—CH(OH)CH2N—R3R4, —CH2P(O)OR3OR4, —S—CF3, —SO—CH3, —SO2CF3, —SO2—(CH2)n—N—R3R4, —SO2—NR3R4, —SO2R7, —CH—(OH)—CH3 or for or A and B together can form a group R3 and R4, in each case, independently of one another, stand for hydrogen, phenyl, benzyloxy, C1-C12-alkyl, C1-C6-alkoxy, C2-C4-alkenyloxy, C3-C6-cycloalkyl, hydroxy, hydroxy-C1-C6-alkyl, dihydroxy-C1-C6-alkyl, heteroaryl, heterocyclo-C3-C10-alkyl, heteroaryl-C1-C3-alkyl, C3-C6-cycloalkyl-C1-C3-alkyl that optionally is substituted with cyano, or for C1-C6-alkyl that optionally is substituted in one or more places, in the same way or differently, with phenyl, pyridyl, phenyloxy, C3-C6-cycloalkyl, C1-C6-alkyl or C1-C6-alkoxy, whereby the phenyl itself can be substituted in one or more places in the same way or differently with halogen, trifluoromethyl, C1-C6-alkyl, C1-C6-alkoxy or with the group —SO2NR3R4, or for the group —(CH2)nNR3R4, —CNHNH2 or —NR3R4 or for which optionally can be substituted with C1-C6-alkyl, R5 stands for hydroxy, phenyl, C1-C6-alkyl, C3-C6-cycloalkyl, benzoxy, C1-C6-alkylthio or C1-C6-alkoxy, R6 stands for the group R7 stands for halogen, hydroxy, phenyl, C1-C6-alkyl, —C2H4OH, —NR3R4, or for the group R8, R9 and R10, in each case independently of one another, stand for hydrogen, hydroxy, C1-C6-alkyl, C3-C6-cycloalkyl or for the group and n stands for 0-6, as well as the isomers, enantiomers, diastereomers and salts thereof.

4. Use of the compounds according to claim 1, in which R1 stands for hydrogen, halogen, C1-C3-alkyl or for the group —(CH2)nR5 steht, R2 stands for —CH(CH3)—(CH2)n—R5, —CH—(CH2OH)2, —(CH2)nR7, —CH(C3H7)—(CH2)n—R5, —CH(C2H5)—(CH2)n—R5, —CH2—CN, —CH(CH3)COCH3, —CH(CH3)—C(OH)(CH3)2, —CH(CH(OH)CH3)OCH3, —CH(C2H5)CO—R5, C2-C4-alkinyl, —(CH2)n—COR5, —(CH2)n—CO—C1-C6-alkyl, —(CH2)n—C(OH)(CH3)-phenyl, —CH(CH3)—C(CH3)—R5, —CH(CH3)—C(CH3)(C2H5)—R5, —CH(OCH3)—CH2—R5, —CH2—CH(OH)—R5, —CH(OCH3)—CHR5—CH3, —CH(CH3)—CH(OH)—CH2—CH═CH2, —CH(C2H5)—CH(OH)—(CH2)n—CH3, —CH(CH3)—CH(OH)—(CH2)n—CH3, —CH(CH3)—CH(OH)—CH(CH3)2, (CH2OAC)2, —(CH2)n—R6, —(CH2)n—(CF2)n—CF3, —CH(CH2)n—R5)2, —CH(CH3)—CO—NH2, —CH(CH2OH)-phenyl, —CH(CH2OH)—CH(OH)—(CH2)nR5, —CH(CH2OH)—CH(OH)-phenyl, —CH(CH2OH)—C2H4—R5, —(CH2)n—C≡C—C(CH3)═CH—COR5, —CH(Ph)—(CH2)n—R5, —(CH2)n—COR5, —(CH2)nPO3(R5)2, —(CH2)n—COR5, —CH((CH2)nOR5)CO—R5, —(CH2)nCONHCH((CH2)nR5)2, —(CH2)nNH—COR5—CH(CH2)nR5—(CH2)nC3-C10-cycloalkyl, —(CH2)n—C3-C10-cycloalkyl, C3-C10-cycloalkyl; C1-C6-alkyl that optionally is substituted in one or more places, in the same way or differently, with hydroxy, C1-C6-alkyl or the group —COONH(CH2)nCH3 or —NR3R4; C3-C10-cycloalkyl, —(CH2)n—O—(CH2)n—R5, —(CH2)n—NR3R4, —CH(C3H7)—(CH2)n—OC(O)—(CH2)—n—CH3, —(CH2)—R5, —C(CH3)2—(CH2)n—R5—C(CH2)n(CH3)—(CH2)nR5, —C(CH2)n—(CH2)nR5, —CH(t-butyl)-(CH2)n—R5, —CCH3(C3H7)—(CH2)nR5, —CH(C3H7)—(CH2)n—R5, —CH(C3H7)—COR5, —CH(C3H7)—(CH2)n—OC(O)—NH—Ph, —CH((CH2)n(C3H7))—(CH2)nR5, —CH(C3H7)—(CH2)n—OC(O)—NH—Ph(OR5)3, —NR3R4, —NH—(CH2)n—NR3R4, R5—(CH2)n—C*H—CH(R5)—(CH2)n—R5, —(CH2)n—CO—NH—(CH2)n—CO—R5, —OC(O)NH—C1-C6-alkyl or —(CH2)n—CO—NH—(CH2)n—CH—((CH2)nR5)2, or for C3-C10-cycloalkyl, which is substituted with the group or for the group X stands for oxygen or for the group —NH, —N(C1-C3-alkyl) or or R2 stands for the group or X and R2 together form a group A and B, in each case independently of one another, stand for hydrogen, hydroxy, C1-C3-alkyl, C1-C6-alkoxy or for the group —S—CH3, —SO2—C2H4—OH, —CO—CH3, —S—CHF2, —S—(CH2)nCH(OH)CH2N—R3R4, —CH2PO(OC2H5)2, —S—CF3, —SO—CH3, —SO2CF3, —SO2—(CH2)n—N—R3R4, —SO2—NR3R4, —SO2R7, —CH(OH)—CH3, —COOH, —CH((CH2)nR5)2, —(CH2)nR5, —COO—C1C6-alkyl, —CONR3R4 or for A and B together can form a group R3 and R4, in each case independently of one another, stand for hydrogen, phenyl, benzyloxy, C1-C12-alkyl, C1-C6-alkoxy, C2-C4-alkenyloxy, C3-C6-cycloalkyl, hydroxy, hydroxy-C1-C6-alkyl, dihydroxy-C1-C6-alkl, heteroaryl, beterocyclo-C3-C10-alkyl, heteroaryl-C1-C3-alkyl, C3-C6-cycloalkyl-C1-C3-alkyl that optionally is substituted with cyano, or for C1-C6-alkyl that optionally is substituted in one or more places, in the same way or differently, with phenyl, pyridyl, phenyloxy, C3-C6-cycloalkyl, C1-C6-alkyl or C1-C6-alkoxy, whereby the phenyl itself can be substituted in one or more places, in the same way or differently, with halogen, trifluoromethyl, C1-C6-alkyl, C1-C6-alkoxy or with the group —SO2NR3R4, or for the group —(CH2)nNR3R4, —CNHNH2 or —NR3R4 or for which optionally can be substituted with C1-C6-alkyl, R5 stands for hydroxy, phenyl, C1-C6-alkyl, C3-C6-cycloalkyl, benzoxy, C1-C6-alkylthio or C1-C6-alkoxy, R6 stands for the group R7 stands for halogen, hydroxy, phenyl, C1-C6-alkyl, —(CH2)nOH, —NR3R4 or the group R8, R9 and R10 stand for hydrogen, hydroxy, C1-C6-alkyl or for the group —(CH2)n—COOH, and n stands for 0-6, as well as the isomers, diastereomers, enantiomers and salts thereof.

5. Use of the compounds comprising general formula I, in which R1 stands for halogen or C1-C3-alkyl, X stands for oxygen or —NH, A stands for hydrogen, B stands for hydroxy, —CO-alkyl-R7, —S—CHF2, —S—(CH2)nCH(OH)CH2N—R3R4, —S—CF3, or —CH—(OH)—CH3, or A and B, independently of one another, can form a group R2, R3, R4, R7 and R8 have the meanings that are indicated in general formula I, as well as the isomers, diastereomers, enantiomers and salts for the production of a pharmaceutical agent for contraception.

6. Use of the compounds comprising general formula II

7. Use of the compounds according to claim 5in which R1 stands for a C1-C10-alkyl group, R2 stands for hydrogen, X stands for NR2 or CHNR2R3, R1 and R2, independently of one another, stand for hydrogen, alkyl, substituted alkyl, cycloalkyl or substituted cycloalkyl, and n=2, as well as the isomers, enantiomers, diastereomers and salts thereof.

8. Use of compounds according to claim 6, comprising general formula IIa,

9. Use of the substances according to claim 1 for the production of a pharmaceutical agent for the selective modulation of germ cell maturation.

10. Use of the substances according to claim 8, wherein the germ cells are female germ cells.

11. Use of the substances according to claim 8, whereby the germ cells are male germ cells.

12. Use of the substances according to claim 1 for the production of a pharmaceutical agent for reduction of sperm maturation.

13. Use of the substances according to claim 1 for the production of a pharmaceutical agent for influencing the implantation.

14. Use of the substances according to claim 1 for the production of a pharmaceutical agent for influencing egg cell maturation in vivo.