Claims
- 1. A method for reducing the number of viable cells in a microbial population, the method comprising:
contacting said microbial population with an antimicrobial formulation comprising granulysin as an active agent for a period of time sufficient to reduce the number of viable cells.
- 2. A method according to claim 1, wherein the number of viable cells in said microbial population is reduced by at least 90%.
- 3. A method according to claim 1, wherein the number of viable cells in said microbial population is reduced by at least 99%.
- 4. A method according to claim 1, wherein said microbial population comprises bacteria.
- 5. A method according to claim 1, wherein said microbial population comprises a fungal pathogen.
- 6. A method according to claim 1, wherein said contacting comprises administration in vivo to an infected host.
- 7. A method according to claim 1, wherein said contacting comprises administration in vitro.
- 8. A method according to claim 1, wherein said granulysin is human granulysin.
- 9. A method according to claim 8, wherein said human granulysin comprises the amino acid sequence as set forth in SEQ ID NO:1.
- 10. A method according to claim 8, wherein said human granulysin comprises an amino acid sequence substantially similar to that set forth in SEQ ID NO:1.
- 11. A method according to claim 1, wherein said antimicrobial formulation further comprises a second antimicrobial agent.
- 12. A method according to claim 11, wherein said second antimicrobial agent is an antibiotic.
- 13. A method according to claim 11, wherein said second antimicrobial agent is perforin.
- 14. A method according to claim 13, wherein said microbial population comprises Mycobacterium tuberculae.
- 15. An antimicrobial pharmaceutical composition, comprising:
granulysin in an amount effective to reduce the number of viable cells in a microbial population; and a pharmaceutically acceptable carrier.
- 16. An antimicrobial pharmaceutical composition according to claim 15, wherein said amount is effective to reduce the number of viable cells in a microbial population by at least about 90%.
- 17. An antimicrobial pharmaceutical composition according to claim 15, wherein said amount is effective to reduce the number of viable cells in a microbial population by at least about 99%.
- 18. An antimicrobial pharmaceutical composition according to claim 15, further comprising a second antimicrobial agent.
- 19. An antimicrobial pharmaceutical composition according to claim 18, wherein said second antimicrobial agent is an antibiotic.
- 20. An antimicrobial pharmaceutical composition according to claim 18, wherein said second antimicrobial agent is perforin.
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
[0001] This invention was made with Government support under Grant no. DK35008, awarded by the National Institutes of Health. The Government may have certain rights in this invention.
Provisional Applications (1)
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Number |
Date |
Country |
|
60064265 |
Nov 1997 |
US |