Claims
- 1. A method of producing a reflection of DNA fragments, comprising:
(a) contacting a first collection of DNA fragments with a second collection of DNA fragments under conditions such that hybridization between the first collection of fragments and the second collection of fragments can occur, thereby forming heteroduplex double stranded DNA fragments having a first strand of DNA from the first collection of DNA fragments and a second strand of DNA from the second collection of DNA fragments; wherein the second collection of DNA fragments is biotinylated and has at least 10% of the thymidine residues present replaced with uracil; (b) contacting the hybridized biotinylated fragments of step (a) with immobilized streptavidin under conditions such that the biotinylated fragments are bound by the streptavidin; (c) rinsing away any DNA fragments not bound to the streptavidin, thereby purifying the biotinylated fragments; (d) releasing the biotinylated fragments from the streptavidin; (e) specifically degrading the strands of DNA from the second collection of fragments by contacting the product of step (d) with UDG followed by N,N′-Dimethylethylene-diamine; and (f) synthesizing double stranded DNA from the first strand using the first strand as template.
- 2. A reflection of DNA prepared according to the method of claim 1.
- 3. A method of hybridizing nucleic acids from one or more samples to an array of probe DNA immobilized on a surface of a solid phase comprising: contacting said array, containing or suspected of containing sequences complementary to nucleic acids from one or more samples, with nucleic acids from one or more samples, under conditions such that hybridization between the nucleic acids and probe DNA can occur, wherein the one or more sample nucleic acids are or are derived from a reflection prepared according to the method of claim 1.
Parent Case Info
[0001] This application claims priority of U.S. Provisional Application No. 60/301,193, filed Jun. 27, 2001, incorporated herein in its entirety.
Government Interests
[0002] This invention was made with Government support under Contract No. R33-CA81674-03 awarded by the National Institutes of Health. The Government has certain rights to this invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60301192 |
Jun 2001 |
US |